LOCUS       DAOQAN010000001         9287 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_8765_length_9287_cov_2.919302, whole genome shotgun sequence.
ACCESSION   DAOQAN010000001 DAOQAN010000000
VERSION     DAOQAN010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 9287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9287
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8765_length_9287_cov_2.919302"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /gene="secF"
                     /locus_tag="PKO10_00005"
     CDS             complement(<1..203)
                     /gene="secF"
                     /locus_tag="PKO10_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012225.1"
                     /note="forms a complex with SecD and YajC; SecDFyajC
                     stimulates the proton motive force-driven protein
                     translocation; seems to modulate the cycling of SecA by
                     stabilizing its membrane-inserted state and appears to be
                     required for the release of mature proteins from the
                     extracytoplasmic side of the membrane; in some organisms,
                     such as Bacillus subtilis, SecD is fused to SecF; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HNW65574.1"
                     /translation="MEIFNNNKTYDFMGKKVAFLTISGILIIASLVLLFTRGLNYGID
                     FVGGTIVQVKYEQAAPLDKIREV"
     gene            complement(203..1768)
                     /gene="secD"
                     /locus_tag="PKO10_00010"
     CDS             complement(203..1768)
                     /gene="secD"
                     /locus_tag="PKO10_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HNW65575.1"
                     /translation="MKIFNFKLTIFLISIIFGVFFAIPSLMQTDYGKKVNLGLDLQGG
                     LHMLLGVNTEEAVASKIKSIATAIKYFSDDEELLIDGLTVSDNSIYFSILDIDEMAKM
                     DKMLSEIKGLDIVKQDLEYKVSLNAEEIIKTKDYSVAQAVETIRNRLDQFGLSEPTVV
                     RQGDSDIVVQLPGIKTASDEKAARDLISKAAKLELMAVDEERMDRVYQMTSKEATAYG
                     DVILEDTNDSNKKYLVKEIPILDGSQVVDAQVAFSQSNQPIINFTLNSAGARIFGDFT
                     AKSVGKRLAIVLDGKVYSAPNINERIGGGSGQISGGFTVQEAGNVAIALRSGALLASV
                     TLLEKRSVGPSLGADSIKASMIALVSGTALIFIFMMFYYRRAGIIANIALIANVFILL
                     AVIALFGATLTLPGMAGIILTLGMAIDANVIINERIREVLRTGASVQKAIEDGYSNAI
                     RAIMDGNITTFLVAIVLYAYGSGAIKGFAVTISIGILTSMLTSIVGTHGIYQAIMPKI
                     AKDKNNKKWFGVA"
     gene            complement(1830..2096)
                     /gene="yajC"
                     /locus_tag="PKO10_00015"
     CDS             complement(1830..2096)
                     /gene="yajC"
                     /locus_tag="PKO10_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014473242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HNW65576.1"
                     /translation="MQGDLLTSLLPLVALFAIFYFLIIRPQQKQAKAHKEMIINLKKG
                     DKIVTNGGLMVEVVKVEDAFFVVKNSDNSEMKLAKEFVARLITE"
     gene            2125..3357
                     /locus_tag="PKO10_00020"
     CDS             2125..3357
                     /locus_tag="PKO10_00020"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008934.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HNW65577.1"
                     /translation="MFLLKTEYFNKNLIIKGLITACLLSAFIYLSYFGFEIKLINTLF
                     GLYGIYLLLTIPRVSLFYAGFFTGILWCYWMSVSLQYYDITYIAPFLLLGIGLVFGTI
                     FALFAVINKLSFRILMIFGFLFISPFGFNWLKLELIFIDSYLSTTKFAFFLILISLYL
                     VIKLKRLKVLAILPLLFAFHSEKGEFIDTPKAKIYMPQMYINQDLKWDKEYLKTLNDE
                     NFKQIFDAIDKGYTLVVLPETAFSVALNKYPSLNNMLLELSNKIDIVTGALYVEDNQI
                     FNASYFYSKNSVSIAKKVVLVPFGEEIPLPKFFVDLINDIFYNGATDYSKASSPTDFI
                     IQGEKYRNAICYEGTTDKIFENLGDTKYMIMISNNAWFTPSIEPTLQHLLLKYYSKKY
                     SVTIFHVVNGSENRIYRP"
     gene            complement(3370..4608)
                     /locus_tag="PKO10_00025"
     CDS             complement(3370..4608)
                     /locus_tag="PKO10_00025"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HNW65578.1"
                     /translation="MELQFHIQKMYINKSKINIPKLLIDILTTLQDSGYKPYLVGGCV
                     RDFLLNKAVKDFDIEVFGIENLENLKLILEKYTKVHDIGKSFGVLKINIDDFDIDFSI
                     PRVEKKVGKTHKSFEIKLLSNLNIKKAAKRRDFTINAIYYDYFKDSFIDPFDGIKDLK
                     KRKIRYIDKKSFVEDSLRVFRAFGFASRFDFKITKKTKQLLKTIIKSGELNNLSKERV
                     FEELKKLLLKSKKPSVGLKLLDEFQIFKISFLKKYKAIDKLSKIFENRDIDLKRALVL
                     YFVVLLKDEKEEDVFEFLSKITSDKKFINSVESLCKESLENDIISLKKQSLNIVLEDL
                     IFVEMAFGNKDIDLILQKCEESDILNKALKPHIMGVDLLDLGFTPSGDFRNMLNFAMD
                     LQIKENLSKDDLKQRLKKNF"
     gene            complement(4556..5431)
                     /locus_tag="PKO10_00030"
     CDS             complement(4556..5431)
                     /locus_tag="PKO10_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HNW65579.1"
                     /translation="MSIEKNIEKVVDQNVEQITRDFKYFIPDNIVEIAIGYSISFVFA
                     LLIFFIGKWISKSVVKILGKALRKVGGVDETLVKFLENIVYYALLTVVIIAALNKLGI
                     ATTSFLAILGAAGLAVGLALKDSLGNFASGVMIVLFKPFKAGDSVVAGGVSGTVTEVT
                     IFNTVFLTADNQKIIVPNSSITSGSITNVNANNTRRVDIVVAISYEDSIKNAKDVLTN
                     IINSNPKVLKDKGFGISVTDLAETSVKLGVNVWAKSSDYGSLKAELLEEIKIKFDEVG
                     ITIPYSKNVYQQIKN"
     gene            complement(5444..7147)
                     /locus_tag="PKO10_00035"
     CDS             complement(5444..7147)
                     /locus_tag="PKO10_00035"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HNW65580.1"
                     /translation="MKFSKLFIPTTKEMPSDASLPSHQFLVRGGFIAQTGAGIYDFMP
                     LGKIVLEKIRAIVKKEMDEAGANEVQFGFVTPLSLWSESGRALTMGSELLRFKDRKNG
                     EYVLSPTNEEAVVNMVKNRVTSYKDLPLHLYQINTKFRDEARPRFGLMRGREFLMKDG
                     YSFHSCEEDLVREFNLMEETYKKIYTKLGLDFRVVEADSGAIGGSGSKEFHVLASSGE
                     DTIVVCDGCNYAANIEAAKREAKKYEFKEGELKKVETPNCTTIEDVANFLSVSKEQTI
                     KAVIKKAIFKDESKIVVFFVRGSDELEDTKAQNSVDALELIDATLDEIRDAGVVAGYC
                     GLVDLPKDILVVVDSELENSQGMVCGGNEDNFHFINVDLKVIENIKYFDLIAVKEEDT
                     CACCGGKLSYTKGIEAGHIFQLGDKYSKAMNATFLDENGKAKPFIMGCYGIGVSRLVA
                     AVIEQNHDEKGCIWTKSTTPFMVDIIVSNSKNEDEKNMGEKIYEELKASNVEVILDDR
                     VNARFGFKIGDFELLGFPYAIIIGKKLLDGIVEIVDRKTLLKTEVKIEEVVSEILNLI
                     K"
     gene            complement(7185..8474)
                     /gene="hemA"
                     /locus_tag="PKO10_00040"
     CDS             complement(7185..8474)
                     /gene="hemA"
                     /locus_tag="PKO10_00040"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HNW65581.1"
                     /translation="MSYLVISFSHKNLDIKQREKLAFNSDEEKARFIRKLLEAQITSE
                     LVLLSTCNRVEIIAKSSNIKQSSKNIIENLASYSSLDFEFLYDRADIYDGDGAVHHLF
                     SVASALDSLVIGETQIVGQLKDAFRFSQSFGFCGEAMSKVMHYAFKCAAQVRTATSLG
                     TGSVSVASTAVAKAKELVGDSKGVNALVIGAGLMSELAVKHLISSGFDVTITSRDIKK
                     AQNLADSFEASIKVEPYSKLEELLEIMPVMITATSAPYPIITQENTPSSSISRYWFDI
                     AVPRDIDENISMFDLDIYSVDDLQEIVNSNMTQRSAQAKEAYSIVGRSTLEFFDWLKS
                     LDIEPIIKNLYLKGDSIIEKKVQNAIKKGFIPKEHEDNIKKLCQTIMTEYLHNPSKQL
                     KNISRNMECDLVVSSVQSMFTKQNSDTNFKCEHLSKN"
     gene            complement(8476..>9287)
                     /locus_tag="PKO10_00045"
     CDS             complement(8476..>9287)
                     /locus_tag="PKO10_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNW65582.1"
                     /translation="KMLRSKLILKIAGVNSETIKLCAVVEMIHAASLLHDDVIDEADT
                     RRGKPSINALYDNKTSIMFGDILYSRAFTELSQMDKKVAYHVSNAVTELSIGEMMDVD
                     LTQSFNSSYDKYLTMIYKKTASLIEASARSAAILIGCDDEKQERYASYGKNLGLAFQM
                     VDDILDITQSSQTLGKPAMLDFKEGKVTIPYLYLYERVEDKEYLKSLYKKELNQKELD
                     YLKEKLNSTNALSDAIKEAKNIGLEAINSIKDEKNSEDLINIMKAMIERDF"
BASE COUNT         3132 a         1653 c          970 g         3532 t
ORIGIN      
        1 aaaacttctc ttattttatc taaaggtgca gcttgttcat attttacttg aacaatagtt
       61 cctccaacaa aatcaatacc atagtttaaa cctcttgtaa atagtaacac aagtgaagct
      121 ataatcaaaa ttccagatat agttaaaaaa gcaacttttt tacccataaa atcataagtt
      181 ttattgttgt taaaaatctc cattatgcta ctccaaacca ttttttattg tttttatctt
      241 ttgcaatttt tggcattatt gcttgataga taccatgtgt tcctacaata gatgtaagca
      301 ttgaagttaa aatccctata gaaatagtta cagcaaaacc tttaatagct ccacttccat
      361 aagcatacaa tacaatagca actaaaaaag ttgtaatatt tccatccata atagctctaa
      421 tagcatttga ataaccatct tctatagctt tttgaacact tgcacccgtt cttaaaactt
      481 ctctaattct ctcatttata ataacatttg catcaatagc catacctaat gtaagaataa
      541 ttccagccat acctggtagt gttaaagttg caccaaaaag agcaattact gctaaaagaa
      601 taaatacatt tgcaattaaa gctatatttg caataattcc agctcttcta taataaaaca
      661 tcataaatat aaaaattaaa gctgttccag agactaaagc tatcattgaa gcttttatac
      721 tatcagcccc caaagatggt ccaacacttc ttttttccaa aagagttacg cttgccaaaa
      781 gtgctccact tcttaaagca atagcaacat ttccagcttc ttgtactgta aaccctccag
      841 aaatttgacc acttccacca ccaattctct catttatatt tggtgcagag taaacttttc
      901 catctaaaac aattgccaac ctttttccta cacttttagc agtaaaatca ccaaaaattc
      961 tagctcccgc actatttaaa gtgaaattta taattggttg atttgattga ctaaaagcaa
     1021 cttgagcatc tacaacttga cttccatcca aaattggtat ctcttttaca agatattttt
     1081 tatttgaatc atttgtatct tctaaaataa catctccata tgcagttgct tctttgcttg
     1141 tcatttggta aactctgtcc attctctctt catcaactgc cataagctca agttttgcag
     1201 cttttgagat taaatctctt gctgcttttt catcactagc tgttttaatt cctgggagtt
     1261 gaacaactat atcactatca ccttgtctta caactgttgg ttcactaagc ccaaattggt
     1321 caagtctatt tctaatagtc tcaacagctt gagcaacaga gtaatctttt gtttttatta
     1381 tctcttcggc atttagagaa actttatact ctaaatcttg tttaactata tctaaaccct
     1441 ttatttctga gagcatttta tccattttag ccatttcatc aatatctaaa attgagaaat
     1501 aaatagagtt atcactaaca gtcaatccgt caataagcaa ctcttcatca tcactaaaat
     1561 atttaatagc agttgcaatt gattttattt ttgaagctac cgcctcttca gtgtttacac
     1621 ccaaaagcat atgaagtcca ccttgaaggt caagtcctag atttactttt ttaccataat
     1681 ctgtttgcat taaagatgga atagcaaaaa aaacaccaaa tataatacta attaaaaaaa
     1741 ttgtgagttt aaaattaaaa attttcaata taaatcctta tttataggga gagaatttaa
     1801 gatatgaaaa tcatatctta aataataaat tattcagtaa taagtctagc aacaaactct
     1861 ttagccaatt tcatctcaga gttatcacta ttttttacta caaaaaatgc atcttcaact
     1921 tttactactt ctaccattaa gccaccattt gttacaatct tatcaccttt tttaaggttt
     1981 atgatcatct ctttatgagc ttttgcttgt ttttgttgtg gtctaattat taaaaaatag
     2041 aatattgcaa aaagtgcaac cagaggtagt aatgaagtca ataaatcgcc ttgcatcgaa
     2101 tttccttttt attgtgggtt tagtatgttt ttattaaaaa cggaatattt taacaaaaat
     2161 ttaataataa aaggcttgat tacagcttgc ctacttagtg cttttatata tttatcatat
     2221 tttggatttg aaattaaatt aatcaataca ctatttggac tttatggaat atatctttta
     2281 ctaacaattc ctagagtttc tctattttat gcaggattct ttacaggaat tttatggtgt
     2341 tattggatga gtgttagctt acaatactat gatattacct atatagctcc atttttactt
     2401 ttaggaattg gtcttgtttt tggaactatt tttgctcttt ttgcagttat aaataaactc
     2461 tcttttagaa ttttaatgat ttttggattt ttatttatct ctccatttgg ttttaattgg
     2521 ttaaaacttg agcttatatt tattgattca tatttaagca ctacaaaatt tgcatttttt
     2581 ttaattctta tatcgcttta tcttgtaata aaattaaaaa gattaaaagt actagctatt
     2641 ttaccactac tatttgcttt tcatagtgaa aaaggtgaat ttatagatac tccaaaagca
     2701 aaaatatata tgcctcaaat gtatataaat caagatttaa aatgggataa agagtattta
     2761 aaaactttaa atgatgaaaa tttcaaacaa atttttgatg ctattgacaa gggctatact
     2821 ttagttgttt tacctgaaac tgctttttct gttgcactaa ataaatatcc tagtttaaac
     2881 aatatgcttc ttgaattatc aaataaaata gatatagtaa ctggtgcttt atatgtagaa
     2941 gataaccaaa tttttaatgc ttcatatttt tatagtaaaa atagtgtatc tatagcaaaa
     3001 aaagttgttt tagtaccttt tggtgaagag atccctcttc ctaaattctt tgttgatttg
     3061 attaatgata ttttctataa tggagctact gattactcaa aagcaagctc tccaaccgat
     3121 tttataattc aaggagagaa atatagaaat gcaatttgtt atgaagggac aacagataaa
     3181 atatttgaaa atttaggcga tactaaatat atgattatga tttcaaataa tgcttggttt
     3241 actccttcaa tagagccaac tttacagcat ctacttttaa aatattactc aaaaaaatat
     3301 agcgttacaa tatttcatgt tgtaaatgga agtgaaaata gaatttatag accttaagag
     3361 gattttattt taaaaattct tcttaagtct ttgttttaaa tcatcttttg aaagattctc
     3421 ttttatttgt aaatccattg caaaattcaa catatttcta aaatcaccac taggagtaaa
     3481 acctaaatca agcaaatcta ctcccataat atgtggtttt aatgctttat ttaaaatatc
     3541 agactcttca catttttgta aaattaaatc aatatcttta tttccaaatg ccatctcaac
     3601 aaagattaaa tcttccaaaa ctatatttaa agattgtttt tttaaactta taatgtcatt
     3661 ttccaaactc tctttacata aagattctac agaatttata aactttttat cactggtaat
     3721 ttttgataaa aattcaaaaa catcttcttc tttttcatct tttagtaaaa caacaaaata
     3781 taaaactaat gcccttttta aatctatatc tctattttca aaaatttttg agagtttatc
     3841 tatagcttta tattttttta aaaaagatat tttaaatatt tgaaattcat ccaaaagttt
     3901 taaaccaaca cttggctttt ttgattttaa aagaagtttt tttaactctt caaaaactct
     3961 ctcttttgat aaattattca attcaccact ttttattata gtttttaaaa gttgttttgt
     4021 cttttttgtt attttaaaat caaatcttga agcaaaacca aaagctctaa aaactcgtaa
     4081 actatcctct acaaaacttt ttttatctat atatcttatt tttctttttt ttaaatcttt
     4141 tattccatca aaaggatcta taaaactatc tttaaaatag tcgtaatata tagcatttat
     4201 agtaaaatct cttctttttg cagctttttt tatatttaaa tttgaaagaa gttttatctc
     4261 aaaactttta tgagtttttc ctactttttt ctctactcta ggaatagaaa aatcaatatc
     4321 aaaatcatcg atatttattt ttaaaacacc aaaagatttt ccaatatcat gaacttttgt
     4381 atatttttct aaaattaatt ttaagttttc taagttttca ataccaaaaa cttcaatatc
     4441 aaaatcttta acagctttat ttaaaagaaa atctcgtaca caaccaccaa caagatatgg
     4501 cttatagcca ctatcttgta atgttgttaa aatatcaatg agtagttttg gtatattaat
     4561 ttttgatttg ttgatataca ttttttgaat atggaattgt aattccaacc tcatcaaact
     4621 ttattttaat ctcttctagt aattcagctt ttaaagaacc ataatcagat gattttgccc
     4681 aaacattaac acccaatttt acagaagttt cagctaaatc agttacagat attccaaatc
     4741 ctttatcttt taaaactttt ggatttgagt ttataatatt tgttaaaaca tcttttgcat
     4801 ttttaatact atcttcgtaa gagatagcca caacaatatc aactcttctt gtattatttg
     4861 catttacatt tgttatactt ccacttgtaa ttgaagagtt tggtacaatt attttttgat
     4921 tatcagcagt taaaaatact gtattaaaaa ttgtaacttc tgtaacagtt ccactaactc
     4981 ctccagcaac tacactatca cctgctttaa atggtttaaa taaaactatc ataacacctg
     5041 aagcaaaatt tcctaaagag tctttcaaag ctaaaccaac agctaaacca gcagctccta
     5101 aaatagctaa aaatgaagtt gtagcaattc ctaatttatt taaagcagca attataacaa
     5161 cagttaataa ggcataataa acaatgtttt ccaaaaactt aaccaaagtt tcatcaacac
     5221 caccaacttt tcttaaagct ttacctaaaa ttttaactac actttttgaa atccattttc
     5281 ctataaaaaa tattaaaagt gcaaaaacaa aagatataga gtatcctata gctatctcca
     5341 caatattatc tggaataaaa tatttaaaat ctcttgttat ttgctcaaca ttttgatcaa
     5401 caactttctc tatatttttt tcaatactca tatttccacc tttttatttg attaagttta
     5461 agatttcaga aacaacttct tctattttta cttctgtttt aagaagagtt tttctatcca
     5521 ctatttcaac tattccgtcc aataattttt taccaataat tatcgcatat ggaaaaccta
     5581 aaagctcaaa atctccaatt ttaaacccaa atctagcatt tactctatca tctaaaataa
     5641 cttctacatt tgaggctttt aactcttcat aaattttttc acccatattt ttttcatctt
     5701 cattttttga gtttgaaaca ataatatcta ccataaatgg agttgtactt tttgtccaaa
     5761 tacaaccttt ttcatcgtgg ttttgctcaa tcacagcagc aacaagtctt gatactccta
     5821 ttccataaca tcccataata aatggttttg cttttccatt ttcatctaaa aatgtagcat
     5881 tcatagcttt tgagtattta tctcccaact gaaaaatatg ccctgcttct attccttttg
     5941 tatatgatag ttttccacca caacaagcac aagtatcttc ttcttttaca gctatcaaat
     6001 caaagtattt aatattttca ataactttta aatcaacatt tataaaatgg aaattatctt
     6061 catttcctcc acaaaccata ccttgagagt tttctaactc actatctaca actaccaaaa
     6121 tatcttttgg taaatctact aatccacaat atccagcaac aactcctgca tctcttatct
     6181 catcaagagt tgcatcaata agttccaaag catctacact attttgtgct tttgtatctt
     6241 caagctcatc acttcctcgc acaaaaaaca ccacaatttt agattcatct ttaaaaattg
     6301 cttttttaat aactgctttt atagtttgct ctttgcttac acttaaaaaa tttgcaacat
     6361 cttcaattgt tgtacagttt ggagtctcta ctttttttaa ttcaccctct ttaaattcat
     6421 atttttttgc ttctcttttt gcagcttcaa tatttgcagc ataattacaa ccatcacaaa
     6481 caacaattgt atcctctcca ctacttgcca aaacgtgaaa ctctttactt ccacttccac
     6541 caattgcacc actatcagct tcaacaactc taaaatcaag acctaatttt gtatatattt
     6601 ttttgtaagt ctcttccata agattaaact ctcttactaa atcctcttca caagagtgaa
     6661 aagagtatcc atctttcatc aaaaactctc ttcctctcat aagtccaaat cgtggacgtg
     6721 cttcatctct aaactttgta tttatttgat aaagatgaag aggtaaatct ttgtatgaag
     6781 taactctatt ttttaccata tttacaacag cttcttcatt tgtaggactt aaaacatact
     6841 caccattttt tctatcttta aatcttaaaa gctcacttcc catagttaaa gctcgtccag
     6901 attcgctcca aagactaaga ggtgtcacaa aaccaaattg aacctcattt gctcctgctt
     6961 catccatctc tttttttaca atagctctga ttttttctaa aacaattttt cccaaaggca
     7021 taaaatcata aattccagct ccagtttgag caataaatcc accacgaact aaaaattgat
     7081 gagaaggcaa agatgcatca cttggcatct cttttgttgt tggaataaaa agtttactaa
     7141 atttcatata tttttcctat atttttaaaa taatttttga ttttctaatt ttttgataaa
     7201 tgttcacatt tgaagtttgt atcactattt tgttttgtaa acatactttg aacagaactt
     7261 acaaccaaat cacactccat atttcttgaa atatttttaa gctgttttga tggattatga
     7321 agatactcag tcattatagt ttggcaaagt tttttgatat tatcttcatg ctcttttggt
     7381 ataaaacctt tttttatagc attttgaact tttttctcaa ttatactatc accttttaga
     7441 tataaatttt taataattgg ttcaatatca aggctcttta accaatcaaa aaactctaaa
     7501 gtagatctac caactattga gtaagcctct ttagcctgag ctgatctttg tgtcatattt
     7561 gaatttacaa tctcttgtaa atcatctaca ctataaatat ctaaatcaaa cattgatata
     7621 ttctcatcaa tatctcttgg aactgcaata tcaaaccaat atctacttat actagaactt
     7681 ggagtattct cttgtgtaat tattggatat ggagcagatg ttgcagttat catcacaggc
     7741 ataatttcaa gtaactcttc aagtttagaa taaggctcta cttttataga agcttcaaaa
     7801 ctatctgcta gattttgagc ctttttaata tcacgactag ttattgttac atcaaatcca
     7861 gagcttatta ggtgtttaac tgccaattca ctcataagtc cagcacctat aactaaagca
     7921 tttacacctt ttgaatctcc tactaactct tttgcttttg caacagcagt agatgcaact
     7981 gaaacagaac ctgttcccaa acttgtagca gttctcacct gagcagcaca cttaaaagca
     8041 taatgcataa ctttactcat agcctcacca caaaagccaa aactttgaga aaatctaaaa
     8101 gcatctttta gttgtcctac aatttgagtt tcaccaataa ccaaactatc aagtgcagaa
     8161 gcaaccgaaa aaagatgatg cacagcacca tctccatcat aaatatcagc cctatcatat
     8221 aaaaattcaa aatccaaaga tgaataacta gctaaatttt caattatatt tttactactt
     8281 tgttttatat ttgaactttt tgcaataatt tcaactctat tacaagtaga aagtaaaacc
     8341 aattcacttg taatttgtgc ttccaaaagt tttcttataa atcttgcttt ctcttcatca
     8401 ctattaaatg ctaatttttc tctctgtttt atatctaaat ttttatgcga aaaactgatt
     8461 actaaataac tcatcttaaa aatctctttc tatcatagct ttcataatat ttattaaatc
     8521 ttcactattt ttttcatctt ttatagagtt tattgcttca agccctatat tttttgcctc
     8581 ttttatagca tcactcaaag catttgtact atttagtttc tcttttaaat aatcaagctc
     8641 tttttggttt aactcttttt tatacaaaga tttcaaatac tctttatctt caactctttc
     8701 atataaatat agatatggaa tagttacttt tccctcttta aaatcaagca tagcaggttt
     8761 tccaagagtt tggctacttt gtgtaatatc taaaatatca tcaaccatct gaaaagcaag
     8821 acctaaattt tttccataag aggcatatct ctcttgtttt tcatcatcgc aacctatcaa
     8881 aatagctgca cttctagcac tagcttctat taaagatgct gtttttttat atatcattgt
     8941 taaatattta tcatatgaac tattaaatga ttgtgttaaa tcaacatcca tcatctcacc
     9001 aatgctaagc tccgtaactg catttgaaac atgatatgca actttcttat ccatttgaga
     9061 taattctgta aaggctcttg agtataaaat atctccaaac attattgaag ttttattatc
     9121 atacaaagcg ttaatagatg gttttcctct tcttgtatct gcttcatcta taacatcatc
     9181 atgaagtagt gatgctgcat gaatcatctc cacaacagca caaagcttta tagtttcact
     9241 attaacccca gcaattttaa gtataagttt acttcttagc atctttc
//
LOCUS       DAOQAN010000002         7122 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_12828_length_7122_cov_3.263903, whole genome shotgun sequence.
ACCESSION   DAOQAN010000002 DAOQAN010000000
VERSION     DAOQAN010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 7122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7122
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12828_length_7122_cov_3.263903"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..756
                     /locus_tag="PKO10_00050"
     CDS             <1..756
                     /locus_tag="PKO10_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014469346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HNW65583.1"
                     /translation="INPIVKELNTYRTTLLLNLVEACSNNFKTGARSASFFEIGTIFD
                     ENRVESKKIAFVHSGASSLEDVSNSGKPKNIDFFSFAKKVLNTIGEFELEPMTNIDNK
                     FIHPYQSANILIDGKISGFISKLHPSVESDFDLSDTFFAQIDFDSLKNSLVKASSYSK
                     FQASRKDLSIIAPKTLEFKEIKKVINSLNNNLIKQYNLIDIYSDEKLGENESLTIRFT
                     LQSDDKTLEDEDINQVINSILDALKEKLNITLR"
     gene            766..2043
                     /gene="aroA"
                     /locus_tag="PKO10_00055"
     CDS             766..2043
                     /gene="aroA"
                     /locus_tag="PKO10_00055"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HNW65584.1"
                     /translation="MSSFNIKRLNKPFDVVIENIASDKSISHRCAMFSLFSNKTSYIK
                     NYLTAEDTLNTLKIVEQLGAKIKRDGSSVEITPVDNLSEPDDVLDCGNSGTAMRLFCG
                     LLASVDGSFVLSGDKYLRSRPMKRVADPLRNIGANIDGRENGNKAPLFIRGEKYLKPF
                     TYISPVDSAQVKSAMILAALRATNVSKYKENELTRDHTERMLKGMGAEIFTDNEGFIN
                     IKPLTSHLKPLNITVPADPSSGFFFAVAAAISKGSRVVIKNASLNPTRVEAYVVLKKM
                     GAIVNFIEKENVYEPIGDIEIIGNELNGVEVSQNISWLIDELPALSIAMSLAKGTSKV
                     KNAKELRVKESDRIKAVVDNLALCGVDFTEFEDGYEIRGGELKSATIDSYGDHRIAMS
                     FAIAGLNCDMKIDDVECINSSFPNFKEILDSLY"
     gene            2053..2892
                     /locus_tag="PKO10_00060"
     CDS             2053..2892
                     /locus_tag="PKO10_00060"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HNW65585.1"
                     /translation="MKIELASSYGFCFGVKRAIEIAQKYENSATMGPLIHNEDEINRL
                     KKDFNVGLYSQLSDVKPDDTVIIRTHGIPKNDLKELKKSVKKVINATCPFVTTPQNIV
                     KNMSKEGYSILIFGDIEHPEVKGVKSYADDLDDVHIILSVHDLKNIKFKNNKIACVAQ
                     TTKKKETYLEIVNALILKNKEVRVFNTICDATFENQDAARDISIKADIMIVIGGKSSS
                     NTKQLHSICLENCKDSYLIENEQELEISWFKDKEFCGITAGASTPDWVIKKVYEKIKS
                     FKP"
     gene            2957..4612
                     /locus_tag="PKO10_00065"
     CDS             2957..4612
                     /locus_tag="PKO10_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HNW65586.1"
                     /translation="MRMEDIDLGEDFDFEQMLNESFEQSENNSVVDGVIVDINAERVL
                     VDVGQKIEGLLSLSEITTNGEIKYKVGDTIPVMLMGNRGERPSISHKKVLQKEKFNNF
                     IAKHGENFEDVTIEAKIVSVKQRGGFTLEDADGCEYFMPLAQSYMKTIGAIGKTVKAK
                     VIKVNKNQNSIIVSRKKLIEEGKSEKDSKIAKILENKEPINGVIKKITSYGMFVDLGG
                     IDGLVNYNEISYKGPVNPANYYAEGDTVSVIVLSYDKNKQHLSLSIKAALSNPWEEIK
                     DRLEVGDTITVTVSNFESYGAFVDLGNDIEGLLHISELSWNKNIKNPKEILNIGDEIN
                     VEVIELNFEQKRLRVSLKNLQEKPFNKFVNSHKVGDVLKGKIATLTEFGAFVTIGDVD
                     GLLHNEESSWEPNSKCKNLFKKGDEVEVKIIKIDREKENISLSIKEIATSPAKDFQDK
                     YKIGDIVKGAVKDKKDFGLFIKLENNLDGLVRTEDFGPLNIDEVSIGDEIEAVVINID
                     TKKNRVSLSIRRLEQQQERDMLKSVNDDMSMTLGDAIKDQFKK"
     gene            4623..6209
                     /gene="serA"
                     /locus_tag="PKO10_00070"
     CDS             4623..6209
                     /gene="serA"
                     /locus_tag="PKO10_00070"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HNW65587.1"
                     /translation="MSKKTIVVCDHIHEAGLKILEKTEDINYVFAADVDKVKLLDIIK
                     DADVAITRSSTDVDEKFLNAAVNLKAIIRAGVGYDNVDIEGCSKRGIIAMNVPTANTI
                     AAVELTMAHMLSCMRKFPYAHNQLKIDRVWKREDWYGNELYGKKLGVIGFGNIGHRVA
                     LRAKAFEMDVITYDPYIPSTKATDLGITYTTNFDDILACDIITIHTPKNKETIDMISF
                     DEIQKMKDGVVLINCARGGLYNEEALVENLKNGKIAMAGIDVFKKEPATSHPLLDLPN
                     VTVTAHLGANTKESQKEISIQSANNAIESARGISYPNALNLPIDESKIPSFVKPYIDL
                     TQKMAFLLAQISKSEIRAIEVSAEGELSEYVDSLQTFASVGVLSVSSGSSVNYVSANF
                     IAKEKGIELSTKALTNSSGYKNKVTIKITTSKGVKNISGTVFGEDVQRVVDLDGFKID
                     VDPKGKMIIMKNKDIPGVVGKVGNILGENGINISDFRLSRGKEGTALAVILIDEKANS
                     KVISELDNLEASIAVAYAEI"
     gene            6219..6779
                     /gene="efp"
                     /locus_tag="PKO10_00075"
     CDS             6219..6779
                     /gene="efp"
                     /locus_tag="PKO10_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNW65588.1"
                     /translation="MAIGMSELKKGLKIEVEGIPYKIVEYQHVKPGKGAAFVRAKIKS
                     FLNGKTIEKTFHAGDKCETPNLQQKQMQFLYDDGELLQFMDTATYEQEGLTYEQVGDA
                     FDWIIDGMQVDMMYFNGKAITVEPPMVVELKIIDTPPNFKGDSQGGRKPATLESGAVV
                     QIPFHILEGDVIRVDTRTGEYLEKVK"
     gene            complement(6825..>7122)
                     /locus_tag="PKO10_00080"
     CDS             complement(6825..>7122)
                     /locus_tag="PKO10_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibD"
                     /protein_id="HNW65589.1"
                     /translation="IYSKNKVFSQNIPLFRVPNRKVIISDDLFKLLDYKFVMVEGVYN
                     LLDILKDKIDFFILIISPKIRNGVNALNELNIDFEILHENYLGDEKIVFLKKKS"
BASE COUNT         2780 a          799 c         1269 g         2274 t
ORIGIN      
        1 attaatccaa ttgtaaaaga gttgaatacg tatagaacta ccctactttt aaatcttgtt
       61 gaagcttgtt caaataattt taaaacaggt gcaagaagtg ctagtttttt tgaaattgga
      121 actatttttg atgaaaatag agttgaaagt aaaaaaattg cgtttgttca tagtggagct
      181 tcatctttag aagatgtaag caattcagga aaaccaaaaa atatagactt tttctcattt
      241 gcaaaaaaag ttttaaatac tattggagag tttgaacttg aacctatgac aaatattgat
      301 aataaattta ttcatcctta tcaaagtgca aatattttaa tagatggaaa aatatcagga
      361 tttatctcta agcttcatcc aagtgttgaa tctgatttcg atttaagtga cacttttttt
      421 gcgcaaatag attttgatag tttaaaaaac agtttagtaa aagcttcttc ttactctaag
      481 tttcaagctt ctagaaaaga tttaagcata attgctccaa aaactttaga gtttaaagag
      541 attaaaaagg ttataaattc gctaaataat aatctaatta agcaatataa cttaattgat
      601 atatatagtg atgaaaaatt aggagaaaat gagagtttaa ctattagatt tactcttcaa
      661 agtgatgata aaacacttga agatgaagat ataaatcaag ttataaactc aattttagat
      721 gctttaaaag agaaattaaa tattacttta agataaggga aaaatatgtc aagttttaat
      781 ataaaaagat taaataagcc ttttgatgtt gttatagaaa atattgcaag cgataaatca
      841 atatctcata gatgtgcaat gttttcacta ttttcaaata aaacttcgta tattaaaaac
      901 tatttaactg ctgaagatac tttaaatact ctaaaaattg tagaacaatt aggagcaaaa
      961 ataaaaagag acggaagcag tgttgaaata actcctgttg ataatttaag tgaacctgat
     1021 gatgtacttg attgtggaaa ctctggaact gctatgagac ttttttgtgg attattagca
     1081 agtgttgatg gctcttttgt tttaagtgga gataaatatt tacgaagtag accaatgaaa
     1141 agagttgcag accctttaag aaatatcgga gcaaatattg atggaagaga gaatgggaac
     1201 aaagcacccc tatttataag aggagaaaag tatttaaaac cttttactta catctctcca
     1261 gttgattcag ctcaagttaa atcagctatg attttagcag ctctaagagc cacaaatgtt
     1321 agtaaatata aagagaatga gcttacacgt gaccatactg agagaatgtt aaaaggtatg
     1381 ggagcagaaa tttttacaga taatgaaggt tttataaata taaaacctct aacatcacat
     1441 ctaaaacctt taaatataac tgttccagca gatccttcta gtggattttt ctttgcggtt
     1501 gcagctgcaa tttcaaaagg ctctagagtt gtaataaaaa atgcatcttt aaaccctaca
     1561 agagttgaag catatgtggt tttaaaaaaa atgggagcca tagtaaactt tattgaaaaa
     1621 gagaatgttt atgaacctat tggcgatatt gaaataattg gaaatgaatt aaatggtgtt
     1681 gaagtaagcc aaaatatatc ttggctaata gatgaacttc cagctcttag tattgcaatg
     1741 agtttagcaa aaggaacatc aaaagtaaaa aatgcaaaag agctaagagt aaaagagagc
     1801 gatagaataa aagcagttgt tgataattta gctctttgtg gtgttgattt cacagagttt
     1861 gaagatggat atgagataag aggtggggag ttaaaaagtg caactataga ctcttatgga
     1921 gatcacagaa ttgccatgag ttttgcaata gctggattaa attgtgatat gaaaatagat
     1981 gacgtagagt gtataaactc atcttttcca aactttaaag agattttaga ctctttgtat
     2041 taaggataaa ttttgaaaat agaactagca agtagctatg gtttttgttt tggtgtaaaa
     2101 agagccatag aaattgcaca aaagtatgaa aatagtgcaa ctatgggacc acttattcat
     2161 aatgaagatg agataaatag attaaaaaaa gattttaacg taggattata ttcacaatta
     2221 agtgatgtga aaccagatga tactgttatt attagaactc atggtatccc taaaaatgat
     2281 ttaaaagagc ttaaaaaaag tgttaaaaaa gttataaatg ctacttgtcc atttgtaaca
     2341 actcctcaaa atatagtaaa aaatatgtct aaagagggtt actctattct tatttttgga
     2401 gacattgagc atcctgaagt aaaaggtgta aaatcttatg ctgatgattt agatgatgtt
     2461 catataattt tatctgtcca tgatttaaaa aatataaaat ttaaaaataa taaaattgct
     2521 tgtgtcgcac aaactacaaa gaaaaaagag acatatcttg agattgtaaa tgctcttatc
     2581 ttaaaaaata aagaggttcg agtttttaat acaatttgtg atgcaacttt tgaaaaccaa
     2641 gatgcggcta gagatatttc aataaaagct gatattatga ttgtaattgg tggaaaaagt
     2701 tcatcaaata caaaacaact tcactctatt tgtcttgaaa attgtaaaga ttcatattta
     2761 attgaaaatg aacaagagct tgaaatttct tggtttaaag ataaggaatt ttgtggaatc
     2821 actgctggtg cgagtacgcc tgactgggtt attaaaaaag tctatgaaaa aataaagagt
     2881 tttaaaccat aatttaagcg atttttttgt acaatcttgc tattttaaat aaactggtaa
     2941 acaaagaagg tacaaaatgc gtatggaaga tatagattta ggtgaagact ttgactttga
     3001 gcaaatgtta aatgagtctt ttgagcaatc agagaataac tctgtggttg atggtgtaat
     3061 tgttgatata aatgctgaaa gagttttagt tgatgttgga caaaaaattg aaggtttatt
     3121 gtctctatca gaaattacaa caaatggtga gataaagtac aaagttggag atacaattcc
     3181 tgttatgctt atgggaaata gaggggaaag acctagtatt tcacataaaa aagtacttca
     3241 aaaagagaag tttaataact ttatagctaa acatggtgaa aactttgaag atgttactat
     3301 tgaagctaaa atagtatctg ttaaacaaag aggtggtttc actcttgaag atgcagatgg
     3361 ttgtgaatac tttatgcctc tagctcaatc ttacatgaaa actattggtg ctattggaaa
     3421 aacagtaaaa gcaaaagtta ttaaagtaaa taaaaatcaa aactcaatta ttgtttcaag
     3481 aaaaaaatta atagaagaag gtaaatctga aaaagatagc aaaattgcta aaattttaga
     3541 gaataaagag ccaataaatg gtgttattaa gaaaattact tcttatggaa tgtttgtaga
     3601 tttaggtgga attgatggtc ttgtaaacta caatgaaatc tcttacaaag gtcctgtaaa
     3661 tcctgcaaat tactacgctg agggagatac tgtttctgtt atcgtattat cttatgataa
     3721 aaataaacaa catttaagct tatcaattaa agctgcttta tcaaatccat gggaagagat
     3781 aaaagataga ttagaagttg gtgatactat tactgtaact gtttcaaatt ttgaatctta
     3841 tggtgctttt gttgatttag gaaatgacat agaaggactt ttacatattt cggaactttc
     3901 atggaataaa aatattaaaa atccaaaaga gattttaaac attggtgatg agataaatgt
     3961 tgaagtaatt gaactaaact ttgagcaaaa aagattaaga gttagtttaa aaaaccttca
     4021 agaaaaacca tttaataaat ttgtaaactc tcacaaagtt ggagatgttt taaaaggtaa
     4081 aattgcaact ttaactgaat ttggagcttt tgttactata ggtgatgttg atggattgct
     4141 tcataatgag gagtctagtt gggagccaaa tagtaaatgt aaaaaccttt tcaagaaagg
     4201 tgatgaagtt gaagttaaaa ttatcaaaat tgatagagaa aaagagaata tttcactttc
     4261 aatcaaagag atagctactt ctcctgcaaa agattttcaa gataaatata aaattggcga
     4321 tattgtaaaa ggtgctgtaa aagataaaaa agattttggt ctatttataa aacttgaaaa
     4381 taacctagat ggacttgtaa gaactgaaga ttttggtcca ttaaatattg atgaagtatc
     4441 aattggtgat gaaattgaag ctgttgttat aaatattgac actaaaaaaa atagagttag
     4501 tttatcaata agaagattag agcaacaaca agaaagagat atgttaaagt ctgtaaatga
     4561 tgatatgtct atgactttgg gtgatgctat aaaagatcaa ttcaaaaagt aggaatattt
     4621 taatgagtaa aaaaacaatt gtagtttgtg atcatattca cgaagctgga ttaaaaattt
     4681 tagaaaaaac tgaagatata aattatgtat ttgcagctga cgttgataaa gtaaaactat
     4741 tagatattat aaaggatgca gacgttgcaa ttacaagatc ttcaacagat gttgatgaaa
     4801 aatttctaaa tgcagcagtt aatttaaaag caattatacg agctggtgtt ggttatgata
     4861 atgttgatat tgaaggttgt agtaaaagag gaataattgc tatgaatgtt ccaactgcaa
     4921 acacaatagc agcagttgaa cttacaatgg cacatatgct atcttgtatg agaaaatttc
     4981 catatgctca taatcaatta aaaattgata gagtttggaa aagagaagat tggtatggaa
     5041 atgagcttta tggtaaaaaa ctaggtgtta ttggttttgg aaatattgga catagagttg
     5101 ctcttagagc aaaagcattt gagatggatg ttattactta tgacccttat attccaagca
     5161 ctaaagcaac agatttaggt attacttata ctacaaattt tgatgatatt ttggcttgtg
     5221 atattattac aattcacact ccaaaaaaca aagagactat cgatatgatt agttttgatg
     5281 agattcaaaa aatgaaagat ggagttgtat taataaactg tgctagaggt ggattatata
     5341 atgaagaagc attagtagaa aacctaaaaa atggaaaaat tgcaatggct ggaattgatg
     5401 tattcaaaaa agagcctgca acttctcatc cactattaga tttaccaaat gttacagtaa
     5461 ctgctcactt aggagcaaat acaaaagaga gtcaaaaaga gatctctatt caaagtgcaa
     5521 acaatgcaat agagagtgct agaggaatat cttatccaaa cgcattaaat cttccaatag
     5581 atgagagtaa aataccttca tttgtaaaac catatataga cttaactcaa aaaatggcat
     5641 ttttattagc acaaattagt aaaagtgaaa ttagagctat tgaagtaagt gctgagggtg
     5701 aactatctga atatgttgac tctttacaaa cttttgcaag tgttggagtg ttaagtgtta
     5761 gttctggaag tagcgtaaac tatgtaagtg caaactttat tgcaaaagag aaaggtattg
     5821 aactatctac taaagcttta acaaatagta gcggatataa aaataaagtt actattaaaa
     5881 taactacatc aaaaggtgta aaaaatatat ctggaactgt atttggtgaa gatgttcaaa
     5941 gagttgttga tcttgatgga ttcaaaattg atgttgatcc aaaaggtaaa atgattatta
     6001 tgaaaaacaa agatattcca ggtgttgttg gaaaagttgg aaatatctta ggtgaaaatg
     6061 gaattaatat ctctgatttt agactaagtc gtgggaaaga gggaactgct cttgcagtta
     6121 ttttaataga tgaaaaagca aactcaaaag taattagcga acttgataat ttagaagcta
     6181 gtattgctgt tgcttatgct gaaatataag gagtaaaaat ggcaattgga atgagcgaat
     6241 taaaaaaggg tcttaagatt gaagttgagg gaattccata taaaattgtt gaataccaac
     6301 atgtaaaacc tggtaaaggt gctgcatttg taagagcaaa aataaaatct tttttaaatg
     6361 gaaaaactat tgaaaaaact ttccatgctg gagataagtg tgaaactcca aatttacaac
     6421 aaaaacagat gcaatttttg tatgatgatg gtgaattatt acaatttatg gatactgcaa
     6481 cttatgagca agaaggatta acttatgagc aagttggaga tgcatttgat tggataatag
     6541 atggaatgca agtagatatg atgtatttca atggaaaagc tattacagtt gaacctccaa
     6601 tggttgttga gcttaaaatt atagatactc caccaaactt caaaggtgac tctcaaggtg
     6661 gtagaaaacc agctacttta gaatcaggtg ctgttgtaca aattcctttt catatacttg
     6721 aaggtgatgt tataagagta gatactagaa ctggtgaata tttagaaaaa gtaaaataat
     6781 taaaaagcta gatttattct agctttttat caaattttaa tatattaact cttctttttt
     6841 aaaaaaacta tcttttcatc tcccaaatag ttctcatgca atatttcaaa atctatattt
     6901 agttcattta aagcatttac cccatttcta atttttgggc ttataattaa aataaaaaag
     6961 tcgattttat cctttaaaat atcaagaaga ttataaacac cttcaaccat tacaaactta
     7021 taatcaagta gtttaaatag atcatcacta atgattactt ttctatttgg tactctaaaa
     7081 agtggtatat tttgagaaaa aaccttattt ttactataaa ta
//
LOCUS       DAOQAN010000003         6417 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_14837_length_6417_cov_3.807922, whole genome shotgun sequence.
ACCESSION   DAOQAN010000003 DAOQAN010000000
VERSION     DAOQAN010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 6417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6417
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14837_length_6417_cov_3.807922"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..780)
                     /locus_tag="PKO10_00085"
     CDS             complement(<1..780)
                     /locus_tag="PKO10_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW65590.1"
                     /translation="MSKLDDILKKSISGKTSDTYVRSGEIDTILEKSRRGTEAAVFNM
                     PKKIEPPAPVAEVEKPGIFTRVRRAAEDFIARPEDRRNAVELPQLPSNFSSLPLEEKK
                     RVSEQFQLQNDEWKKQNNFWHQLPGATAESLPFGTGAIVEFARDNPEAVANVTLEDVL
                     TGLQTTSVETFKGLASTALSIGRGPKGGVKINVPDLKFEAYVPGLGNQKVVIPIGEIS
                     NSQYRSAQRVANGESPAKVILEEGVANNIFGVLMLYGLGNEI"
     gene            complement(784..2202)
                     /locus_tag="PKO10_00090"
     CDS             complement(784..2202)
                     /locus_tag="PKO10_00090"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HNW65591.1"
                     /translation="MATTNTTIGKNISSVPTSTVQQVVGTTVDNKYGPQTTASVKAFQ
                     TANGLVADGIVGPKTSAAILAAQAKTNTGTGNTGTGGSSSDPTITSTDTRAGYDALGN
                     EITNKIKDNIANYDSEGYKSASEEIQKSLDKRISDLERIYQGDVNGIKTAYESASVIQ
                     ADRQNKDYAGRATGLITAGGGFLGATQSQQGVLQNLTETFQQEKNALMAKRDAALQEA
                     ANAFAENSFNIAQLKLKEAKDTEQELYNRQQDFNNQQLDLAKENRAQTEFEYGITDKR
                     AESYANLTDELYKAVTPEQKADIDKRYYPGYLDNLQEVKSKEAEIKTAKDALDLEATI
                     LDMRLKMPYGKSFNLGGVTYTGLKSETSGGTKEEREAATFAKIDELFSNKLNTEGGKL
                     YTIPEPVMINGKMETGVPFTDEQGYLTPEGWLYAVDNFNIDPTELIKRYAGKFNPNEN
                     YANLGNYRLSGEQKKLINKPEI"
     gene            complement(2234..3886)
                     /locus_tag="PKO10_00095"
     CDS             complement(2234..3886)
                     /locus_tag="PKO10_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW65592.1"
                     /translation="MSILFTSVPQKILKQSILGSSTTFSINNIKGWDGEDLEASDFGT
                     VGYGAFMSADKTRLEFFEWDASTIAASVINFNKRGLQFDGDLTTEVDANKLDWTANET
                     YVMLGADTSQHFQWLKEYIDGIAISGSPDMSTTNKGLAEEATQAEVDAKTAAGGTLAR
                     LAVNPSTLRATKYNDYVADAVGTDSYAITVDPVITAYAAGQEFTFKAGTANTGACTLN
                     VCGLGAKDIKKDVSVDLTTGDILQYQIVKVIYDGTNMQLVSIKSNLADSSSITVFTAS
                     GTYTKNSKAKILKVIAFGAGGNGTTGGVGSTRSAGGGGGGARIEKDLIASQVSSTVAV
                     NIAPGGAGSGGAPTSFGSYIKAYNGGNGGGSNSNSGTGGAGGGSAGNGQNGSGELDVL
                     GGLPASVAGAAGISGQGAGAGSGNGRNAECGGGSGGGYDNGDGGSSLYGGGGGGGAEN
                     GSTTGGAGGVSGSYVAGGGGTGGAGTTGGAGGAGQSRSGTGIAGDGGGGGGGVATGTG
                     GAGGAGGIPAGGGGGGGGASSSGAGGAGGAGARGEVIVIEYF"
     gene            complement(3912..5975)
                     /locus_tag="PKO10_00100"
     CDS             complement(3912..5975)
                     /locus_tag="PKO10_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW65593.1"
                     /translation="MAKTLTISQQDFKKGLQVLEDDSKAAFGSAREMLNCIITERGGI
                     APRPGTVLLGAYNDSLFPVRGLYNFKKSRGDNDILTKFYDDEMEFFHPTLKVWSRVKN
                     GFTPDQEFDATYSLVNTDNDDFMYYCNAIDKYSRWSGANTYVTEDLAGGETVIKVNSL
                     LTSQIIYSATATGSSATTLVVSTATYATDMWKNFYIYIPSTGKVRLITGNNGTTITFA
                     TLGSDPGTVAFQIRQLAFPASGTVIYDGTPSTYTSIDVYNQLITPAAHAVTAPVPLTI
                     VPTEYDAAPKGNRIDTLRGRVYVGRVRSGISRDSSGNLQGSSQASGVFVSKLIDPTTF
                     TFAASRVAGEGDLISVVYGGGQINDVAAFEDEIAIYKQDYIELVKYTEDTDDYAIRTP
                     LKPGYGSVARVIQGADDHYFMRPDKQYTSLGRVRTKDLTPQTENIGYVIKRLLDTYNH
                     TSFNGIEFNNRLLSSHKTTDDSPTNDVTLIFNKITKSFEGIWNIGVNNFESFKEEGET
                     SNELVYGEANGANIWKMFQSRKADVRGIDILPYTAKWQSNFFNVLPIKSNIQAVQSVA
                     IEGYISGGTTFTFKLLKNFETDSSLEFTFGGTEEEFQQGTGDIAKFFGSNPFGLVPIG
                     SLSGPESDGRRRFSFIVYFPFIYGQYFSTLFSSSGVDQNWEIIRLSLGLKEDVSVRQA
                     NTKAV"
     gene            complement(5977..>6417)
                     /locus_tag="PKO10_00105"
     CDS             complement(5977..>6417)
                     /locus_tag="PKO10_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW65594.1"
                     /translation="GWNLLKFDMTSLTTVGTPVDTACVYSALYMTKDVSKVSETGYKF
                     DDLVLHNGEISNIYYYSGYGWQTSGGTYIKNSTADSDLLNAGEEEYEIILCKCTELAA
                     DETDEDKVSEKQQKMYKDLSKTYKEGNPSEALIMVSTTVDFVKV"
BASE COUNT         1867 a         1338 c          893 g         2319 t
ORIGIN      
        1 aatttcatta ccaagtccat aaagcattaa tactccaaaa atattatttg caactccttc
       61 ttctaaaata acttttgctg gagattctcc atttgcaact ctttgagcag acctatattg
      121 actattactt atttcaccaa taggaataac aactttttgg tttccaagac caggaacata
      181 tgcttcaaat tttaaatctg gaacatttat ttttactcca cctttaggac ctcttccaat
      241 acttaatgct gttgaagcaa gacctttaaa tgtttctact gatgtagttt gtaaaccagt
      301 taatacatct tcaagtgtta catttgcaac tgcttctgga ttatcacgag caaattcaac
      361 tattgcacca gttccaaaag gaagtgattc tgctgttgct ccaggaagtt gatgccaaaa
      421 attgttttgc tttttccatt catcattttg taattgaaat tgttcactta ctcttttctt
      481 ttcctcaagt ggaagagaac taaaatttga aggaagttgt ggaagttcta cagcatttct
      541 tctgtcttct ggtcttgcaa taaaatcttc agcagctctt ctgacacgag taaagatacc
      601 tggcttttca acttcagcta caggtgctgg tggttctatc ttctttggca tattaaaaac
      661 tgcagcttca gttcctcgcc ttgatttttc taatattgta tctatttctc cagaacgaac
      721 atatgtgtca ctagtctttc cagagataga tttctttaag atgtcgtcta attttgacat
      781 atattaaatt tctggtttat ttattaattt cttttgttcc ccactcaacc tgtaatttcc
      841 taaattagca tagttttcat tagggttaaa tttaccagca tatcttttaa taagttcagt
      901 tggatcaata ttaaaattat ccacagcata taaccatcct tctggtgtta agtaaccttg
      961 ttcatctgtg aatggaacac ctgtttccat tttaccattt atcattactg gttctggaat
     1021 tgtgtataat ttaccacctt ctgtatttaa tttgtttgag aataattcat ctattttagc
     1081 aaaagttgct gcttctcttt cttcttttgt tcctccagaa gtttctgatt taagtcctgt
     1141 atatgttaca ccaccaagat taaacgactt accatatggc atctttaatc tcatatccaa
     1201 aatagtagct tctaaatcaa gagcatcttt tgctgtttta atttcagctt ctttagattt
     1261 aacttcctgt aagttatcta gataaccagg ataatatctt ttatcaatgt ctgctttttg
     1321 ttctggagta acagctttat aaagctcatc tgtaagattt gcataacttt cagctctttt
     1381 atcagtaatt ccatattcaa attctgtttg agctctattc tctttagcaa ggtctaattg
     1441 ttgattattg aaatcttgtt gtctattata aagttcttgc tctgtatctt tagcttcttt
     1501 gagttttagt tgggcgatat tgaaactatt ttctgcaaaa gcatttgcag cttcttgaag
     1561 tgcagcatct cttttagcca taagagcatt cttttcttgt tggaatgttt cagttagatt
     1621 ttgtagaact ccttgctgac tttgagtagc accaaggaaa ccaccaccgg cagttattaa
     1681 accggtagcc cttccagcat aatctttatt ttgtctgtca gcttgaatga cagatgcact
     1741 ctcgtaagct gttttaattc catttacatc accttgataa atcctttcaa gatcacttat
     1801 tcttttatct agtgattttt gtatttcttc acttgcactt ttatatcctt cactatcgta
     1861 attggcgata ttgtctttta ttttatttgt tatttcattt ccaagagcat catacccagc
     1921 acgagtatcg gtgcttgtta ttgttgggtc acttgagctt cctcctgttc ctgtattacc
     1981 tgttccagta tttgtttttg cttgtgcagc taaaatagca gctgaagttt ttggtccaac
     2041 aataccatca gcaacaagtc cattagctgt ttggaaagct ttaactgaag cagttgtttg
     2101 aggaccatat ttattatcaa cagtagttcc aacaacttgt tgaactgttg atgttgggac
     2161 tgacgatata ttttttccta ttgttgtgtt tgttgtagcc ataatgtttt ttatattaga
     2221 ttaattatta tggttaaaaa tattctatta ctattacctc tcctcttgct cctgctcctc
     2281 ctgctcctcc tgcaccagaa gatgaagctc ctcctccacc tcctcctcca cctgcgggaa
     2341 tacctccagc tccaccagca ccaccagtac cagttgctac tcctccacct cctccaccac
     2401 catcacctgc gattccagta ccacttcttg attgaccagc tccaccagca ccacctgttg
     2461 ttcctgcacc tccagtgcca ccaccacctg caacataaga acctgaaact ccaccagcac
     2521 caccagttgt tgatccattt tcagctcctc ctccaccacc tccaccgtat aatgaactac
     2581 ccccatcacc attatcataa ccaccaccac taccacctcc acattctgca tttcttccat
     2641 taccactacc agctccagca ccttgaccag aaataccagc agcaccagct actgaagcag
     2701 gtagacctcc taatacatct aattctccag atccattttg tccatttcct gcagaacctc
     2761 ctcctgctcc tcctgtacca ctatttgagt tactacctcc tccatttcct ccattgtatg
     2821 cttttatata agatccaaaa ctagtaggtg ctcctccact gcctgctcct cctggagcta
     2881 tattcacagc cacagttgaa gaaacttgac ttgctattaa atctttttca attctagctc
     2941 ctcctcctcc tccacctgca gatctagtac tcccaactcc acctgttgtt ccatttcctc
     3001 ctgcaccaaa tgctattact ttaagtattt tagctttaga attttttgta tacgtaccag
     3061 aagcagtaaa tacagttatt gaagatgaat cagctaaatt tgattttatt gaaactaatt
     3121 gcatatttgt tccatcataa ataactttta ctatttgata ttgtaaaata tctcctgtag
     3181 ttaaatcaac tgatacatct tttttaatat cttttgcacc taatccacat acatttaaag
     3241 tacaagcacc tgtatttgct gtccctgctt taaaagtaaa ttcttgtcct gctgcataag
     3301 cagtaattac tggatcaact gtaatggcat aactgtctgt tccaacagca tcagcaacat
     3361 agtcattata ttttgtagca cgaagtgttg aaggatttac tgcaagtcta gctaatgttc
     3421 caccagctgc tgtttttgca tcaacttctg cttgtgtagc ttcttctgca agtcctttat
     3481 ttgttgttga catatctgga gaaccagaaa ttgcaattcc atcaatgtat tcttttaacc
     3541 attgaaaatg ttgagatgtg tcagctccaa gcatcacata agtttcattt gcagtccagt
     3601 caagtttgtt tgcatcaact tcagtagtta aatctccatc aaattgaaga cctcttttat
     3661 taaaatttat aacagatgca gcaattgttg aagcatccca ttcaaaaaac tctagacgag
     3721 ttttatctgc actcataaaa gcaccataac caactgttcc aaagtctgat gcttctaaat
     3781 cttctccatc ccaaccttta atattgttta tagagaatgt tgttgacgac ccaagaatac
     3841 tttgtttgag aattttttgt ggaacacttg tgaataaaat tgacatatat tttaattaat
     3901 tactaataat attatactgc tttagtatta gcttgtctaa cactaacatc ttcttttaga
     3961 cctaatgata acctaataat ttcccaattc tgatcaacac cagaacttga aaataatgta
     4021 gaaaagtatt gaccataaat aaatgggaag taaacaataa atgaaaatct tcgtcttcca
     4081 tctgattctg gaccagataa acttccgata ggaactaacc caaaaggatt agacccaaag
     4141 aattttgcaa tatctccagt tccctgttga aattcttctt ctgttccacc aaaagtaaac
     4201 tctaatgatg aatctgtttc aaagtttttt agaagtttga atgtaaaagt tgttcctcca
     4261 gaaatatacc cctcaattgc tacactttga actgcttgaa tgtttgattt tattggtaag
     4321 acattaaaga aattactctg ccattttgct gtataaggaa gaatatctat tcctcttaca
     4381 tctgcttttc ttgactgaaa catcttccag atgtttgcac cattggcttc tccataaaca
     4441 agttcattag atgtttctcc ttcttctttg aatgattcaa aattatttac tccaatattc
     4501 caaataccct caaatgattt tgttatctta ttaaaaatta aagttacatc atttgttggt
     4561 gaatcatctg ttgttttatg tgaagacaat aatctattat taaattctat accattaaat
     4621 gaagtatgat tatatgtatc aagtaatctc tttattacat atccaatgtt ttctgtttgt
     4681 ggtgttaaat cttttgttct aacacgacca agagatgtat attgcttatc tggtcgcata
     4741 aagtaatggt catcagcacc ttgaataaca cgagctactg aaccataccc tggtttaagt
     4801 ggtgttcgta ttgcataatc atctgtatct tctgtatatt ttacaagttc aatatagtct
     4861 tgtttataaa ttgctatctc gtcttcaaaa gcagcaacat catttatttg tccaccacca
     4921 taaacaacag atattaaatc tccttctcct gctactcttg aagctgcaaa agtaaatgtt
     4981 gttggatcaa taagtttaga aacaaatact ccagaagctt gtgaagaacc ttgtaaatta
     5041 ccagaagaat ctcttgatat tccacttctt actctcccga cataaactct acctcgtaga
     5101 gtatctatac gatttccttt tggagcagca tcatattcag tcggaacaat agttaatgga
     5161 actggtgctg ttacagcatg tgctgctggt gtaattaatt gattataaac atcaatactt
     5221 gtatatgtag aaggtgttcc atcataaatt actgttccag aagcaggaaa agctaattgg
     5281 cgaatttgga aagcaactgt tcctgggtca cttccaagag tagcaaatgt tattgttgtt
     5341 ccattatttc ctgttataag gcgaactttt ccagttgaag gaatatatat ataaaaattc
     5401 ttccacatat ctgtagcata agttgcagta gaaacaacta aagttgtagc actactacca
     5461 gttgcagtag cagaataaat tatttgactt gtaagtaaag aattaacttt aattactgtt
     5521 tccccaccag caaggtcttc tgttacatat gtgtttgctc cagaccaacg agaatattta
     5581 tcaattgcat tacagtaata catgaaatca tcattatcag tattaaccaa agaataagtt
     5641 gcatcaaatt cttgatccgg tgtaaaacca ttcttaactc ttgaccatac tttaagtgtt
     5701 gggtgaaaga attccatttc atcatcataa aattttgtaa ggatgtcatt atctcctcta
     5761 gattttttaa agttataaag tcctcttacc ggaaaaagac tatcattata agcacccaat
     5821 aaaactgtcc caggtcttgg agctattcca cctcgttctg taataataca attaagcatt
     5881 tctctagcac taccaaaagc agctttacta tcatcttcga ggacttgtaa acctttttta
     5941 aaatcttgtt gtgaaattgt taatgttttt gccatactat acttttacaa aatctacagt
     6001 tgtagaaacc attattaatg cttcacttgg attaccttct ttatatgttt tacttaaatc
     6061 cttatacatt ttttgttgtt tttctgaaac tttatcttca tctgtttcgt ctgcagctaa
     6121 ttctgtacat ttacaaagta taatttcata ttcttcttct ccagcattta ataaatcaga
     6181 atctgctgtc gagtttttta tataagttcc accagatgtt tgccaaccat atccagaata
     6241 ataataaata ttacttattt ctccattatg aagaactaaa tcatcaaatt tatatccagt
     6301 ttcagaaacc ttacttacat ctttagtcat atacaaagca gaataaacac aagctgtatc
     6361 aactggtgtt ccaactgtgg taagtgaagt catatcaaat tttaataaat tccaacc
//
LOCUS       DAOQAN010000004         6272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_15351_length_6272_cov_3.656587, whole genome shotgun sequence.
ACCESSION   DAOQAN010000004 DAOQAN010000000
VERSION     DAOQAN010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 6272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6272
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15351_length_6272_cov_3.656587"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="PKO10_00110"
     CDS             <1..201
                     /locus_tag="PKO10_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009649874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HNW65595.1"
                     /translation="IQSSMMQLVIASVGALLFSAFILYDTQNIIKGNYDSPVEAALSL
                     YLDFFNLFISLLQILGIMKNNE"
     gene            237..641
                     /locus_tag="PKO10_00115"
     CDS             237..641
                     /locus_tag="PKO10_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013136842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate pyrophosphorylase"
                     /protein_id="HNW65596.1"
                     /translation="MNKNHLRLIDANLNRLREGIRVVEDIFRYIYNNKEISTKLKSLR
                     HIARTKNYYELLETRDVENDVLRESIKSEQNREDLNSILIANFKRAQESSRVLEELTK
                     LSSIEDSENFKYIRYELYNLEIVLTKITSNSK"
     gene            complement(593..1285)
                     /locus_tag="PKO10_00120"
     CDS             complement(593..1285)
                     /locus_tag="PKO10_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014469522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNW65597.1"
                     /translation="MELKNQFSKYAKEYKSYNIIQQICAKSLVRELKSKPKNILELGC
                     GSGQIFSNVDWEFDKYLAIDGSKQMCELHPKAKNLIVKCLNFDSNEFFNEIKNQKFDM
                     VISSSALQWSNDLKKIVEELSKITGEINVVLFTSNTFKTIQNITNQKSPILDENTIKD
                     AFSSYFLCEFETISYKLEFDNKKELFDYIKKSGVSGKSELNFEKAKKLYKEYNLNYLE
                     FEVIFVKTISKL"
     gene            1369..1722
                     /gene="secG"
                     /locus_tag="PKO10_00125"
     CDS             1369..1722
                     /gene="secG"
                     /locus_tag="PKO10_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HNW65598.1"
                     /translation="MTSTLLIVQFVLAVLIVIVVLLQKSSSIGLGAYSGSNESLFGAK
                     GPANFLSKATMALGLVFVLNTLYLGYTYNEERNKSAIDSVKIESLIPSSPSTQNSTTP
                     SAPEAPKAPASVPEK"
     gene            1740..2297
                     /gene="frr"
                     /locus_tag="PKO10_00130"
     CDS             1740..2297
                     /gene="frr"
                     /locus_tag="PKO10_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HNW65599.1"
                     /translation="MLEEVYSQTKEQMEKSIEALRRDYKTLRTGKVNVNILDNIKVDY
                     YGTMTDLSQVGSVLATDATTITINPWEKNLLGIIEKAIQTANIGVNPNNNGEVIKLFF
                     PPMTVEQRQETAKQAKVMTDNAKVAIRNIRQNSNTKIKNLLKDKLITEDDSKKAQDEV
                     QKITDGFVAKADETLKTKEKEILTV"
     gene            2300..2908
                     /gene="pyrE"
                     /locus_tag="PKO10_00135"
     CDS             2300..2908
                     /gene="pyrE"
                     /locus_tag="PKO10_00135"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008335468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HNW65600.1"
                     /translation="MNIEQIYKDANALLEGHFKLSSGNHSQFYLQSAKVLEDPKTAKL
                     LAEALAVEIKKSGIKIDAVCAPALGGLIAGFALATALDVRFIFAERADGEMTIRRGFE
                     VKPGEKYLVCEDIITTGGSALEAAREIVKGGGEVVAYAALANRGFCQRVGSELSRKDN
                     CKLPLDKPLFALADFAFEMYSPDNCPLCKDGSVAIKPGSRGN"
     gene            2908..3381
                     /locus_tag="PKO10_00140"
     CDS             2908..3381
                     /locus_tag="PKO10_00140"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HNW65601.1"
                     /translation="MSKWRDVKQNRVEKNIPKKVLHSSLNFVSIAKRFKAFLTDTFLI
                     TTPILYIVIYLIMGSGQEFADNRSLGWGIIFAVHFIIIAIFWLKNGQTPGLKAYDLKL
                     VDSKTETRVSILQAIIRYSATLFSIISIFFVFTPFVNKEKKTFQDIVSNTKIIEQ"
     gene            3382..4482
                     /locus_tag="PKO10_00145"
     CDS             3382..4482
                     /locus_tag="PKO10_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014469525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNW65602.1"
                     /translation="MLFFNISAFYFFYFAAVGVYVIFLPKVLNDIGYSAFDIGIILAV
                     APLMKFAMPFLFLKHINLNKSMFKKALFLTVLAVTLFYITIEHFYIFMLNNAFLAACL
                     SLILPYLEVTTVTVLGKERYGKARLFGSIGFMLIALVLAKFLTSPFIALHYYLAIVVL
                     TVIFALLLLKNDLEAKINQTGDEVFSFLKHWAFWASIFFMQVSFGGFYNFFTIYELEY
                     GLSLETISYLWAFGVICEILMLYFQAPILKNNLLTIIKFCVGITIFRWLLLYLYPDNL
                     EIVYFTQAIHAFSFALFHSAVVIYLYSLYDNKKLAQQFMFGVGYGLGGFVGAFLAGAT
                     YGEYLFLFSAIFALISFIFVWFLDKKDKVILK"
     gene            complement(4514..5479)
                     /locus_tag="PKO10_00150"
     CDS             complement(4514..5479)
                     /locus_tag="PKO10_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012148109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HNW65603.1"
                     /translation="MKPFQGFLIEKDGTAKELKYKDIESVDKDNKILWLHFDYTSKEA
                     NDWIRNSSNIDSVAVEALLTDETRPRTIVLNDALLIALRGVNLEPNSKPEKMISIRIF
                     ISSNVIISTSRKNILSVIEISENLKKGKGVKSSSEFLVELTYRMIDRMDNVIDKIEDR
                     ADFLEENIIESEHNTAFRTEILKIRRESIILKRYLTPQKEALVKLYNEKLSWIDDYQK
                     IELRETTDQLIRHIEELDTIRDKVILFQEELVNSLTEQMNKKMYILAILSAIFLPLTF
                     LTGLLGINVGGIPGANHENAFYIFIAILLCIVGFQFFIFKKNKWI"
     gene            complement(5489..6226)
                     /locus_tag="PKO10_00155"
     CDS             complement(5489..6226)
                     /locus_tag="PKO10_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014475465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaA family protein"
                     /protein_id="HNW65604.1"
                     /translation="MKILFSPSETKNSGGVEKIFDENSFIFPQLFNKRVEIINYYNEF
                     LQTASISQLEKLFGTKKSDVIEKYRQDIFKSPLLKAIQRYEGVAYDYLSYNNLEKSSQ
                     KYIDDNVLIFSNLFGVLKASDEIPDYKLKQGESFYDLKIDKFYNDNFSKELDKYLEND
                     DILDLRAGFYEKFYVIKKPYKTLKFIKDGKVVSHFAKAYRGEILKIIAQNDIKTFDDF
                     MNLELKNLKLEEIKEQKLKTEIVYSII"
BASE COUNT         2202 a          874 c          832 g         2364 t
ORIGIN      
        1 attcaatcat caatgatgca actagtaatt gcaagtgttg gagctttact tttctctgca
       61 tttattttgt acgatacaca aaatataatc aaaggaaatt atgactctcc agttgaagct
      121 gcactttcac tatatttaga tttctttaat ctatttatat cgcttcttca aattcttgga
      181 attatgaaaa acaacgaata atatataggg ttcacaccct atatatactc tacaatatga
      241 ataaaaatca cttaagacta attgacgcaa atctaaatcg acttagagag ggaattagag
      301 ttgtagaaga tatttttaga tatatctaca ataataaaga gatttctaca aaactaaaaa
      361 gtttaagaca cattgcacga actaaaaact attatgagct tcttgaaact agagatgtag
      421 aaaatgatgt tttaagagaa tctataaaaa gtgaacaaaa tagagaagat ttaaactcta
      481 ttttaatagc aaactttaaa agagctcaag agagttcaag agttttagag gagcttacaa
      541 aattatcatc aatagaagat agtgagaact ttaaatatat tagatatgaa ctctataact
      601 tagagattgt tttaacaaaa ataacttcaa actctaaata gtttaaatta tactctttat
      661 acaatttttt agccttttca aaatttaatt cactttttcc actaacacct gattttttaa
      721 tgtaatcaaa aagctctttt ttattatcaa actctaattt ataagaaata gtttcaaatt
      781 cacaaagaaa atagctagaa aaagcatctt ttattgtatt ttcatccaaa attggagatt
      841 tttgatttgt aatattttga atagttttaa aagtatttga agtaaataaa acaacattta
      901 tttcaccagt aattttactt agctcttcta ctattttttt taaatcattt gaccactgaa
      961 gagctgaaga tgatattacc atatcaaatt tttggttttt tatctcgtta aaaaattcat
     1021 tactatcaaa atttagacat tttactatta aattttttgc ttttggatga agttcacaca
     1081 tctgttttga accatcaatt gctaaatatt tatcaaactc ccaatctaca tttgaaaaaa
     1141 tttgtccact tccacaacct aattccaaaa tattttttgg tttagattta agctctcgaa
     1201 ctaaagattt tgcacagatt tgctgaataa tattgtatga tttatactct tttgcatatt
     1261 ttgagaattg atttttaagt tccatttatc tctttgaatt ttttttaaca ttattttaac
     1321 aagttttaga taaaatagac accatttttt aaaaaggaat tatagattat gacatctaca
     1381 cttttaatag ttcaatttgt tttagcagtg cttattgtaa ttgttgtatt acttcaaaaa
     1441 agctctagta ttggtctagg agcttatagc ggaagtaatg aatcactttt tggtgcaaaa
     1501 ggacctgcta attttttatc aaaagctaca atggctttag gacttgtttt cgtattaaac
     1561 actctttatc taggatatac atataatgaa gagagaaata aaagtgctat tgactctgta
     1621 aaaatcgagt ctttaatacc atcaagtcca tcaactcaaa actcaacaac accgtctgcg
     1681 ccagaagctc caaaagctcc agcttcagtg cctgagaaat aaatctagag gagataatta
     1741 tgttagaaga agtttattca caaacaaaag agcaaatgga gaaatctatt gaagccttaa
     1801 gaagagatta caaaactctt agaactggta aagtaaatgt aaatatttta gataatatca
     1861 aagttgatta ctatggaaca atgactgatt taagtcaagt tggttctgta ttagcaacag
     1921 atgctacaac tattacaata aatccttggg aaaaaaatct tttaggaata attgaaaaag
     1981 ctatacaaac agcaaatatt ggagtaaatc caaataacaa tggagaagta attaaactat
     2041 tcttccctcc tatgacagtt gaacaaagac aagagactgc aaagcaagca aaagttatga
     2101 cggacaatgc aaaagttgct attagaaata ttagacaaaa ctcaaataca aagataaaaa
     2161 atcttctaaa agataaactt ataacagaag atgatagtaa aaaagctcaa gatgaagtac
     2221 aaaaaataac tgatggtttt gttgcaaaag cagatgaaac tttaaaaaca aaagagaaag
     2281 agattttaac ggtttaatta tgaatataga acaaatatat aaagatgcta atgccttatt
     2341 agaagggcac tttaaattaa gtagcggtaa ccattcacaa ttttatcttc aaagtgcaaa
     2401 agttttagaa gatccaaaaa cagcaaaact tttagcagaa gctttagctg ttgaaattaa
     2461 aaaatcagga attaaaattg atgcagtttg cgctcctgca cttgggggat taatagctgg
     2521 atttgcactt gcaactgctt tggatgtaag atttatcttt gcagaaagag ctgatggtga
     2581 gatgactata cgaagaggtt ttgaagtaaa acctggtgaa aaatatcttg tttgtgaaga
     2641 tattattaca actggtggaa gtgctttaga agcagcaaga gaaatcgtaa aaggtggtgg
     2701 tgaagttgtg gcttatgcag ctttagcaaa tagaggtttt tgtcaaagag tgggaagcga
     2761 attatcaaga aaagataact gtaaactacc tttagataaa ccactttttg cattagcaga
     2821 ttttgcattt gagatgtata gtccagataa ctgtccactt tgcaaagatg gtagtgttgc
     2881 tattaaacca ggtagtagag gaaactaatg tcaaaatgga gagatgtaaa acaaaataga
     2941 gtagagaaaa atattccaaa aaaagtttta cactcatctt taaattttgt atcaatagca
     3001 aaaagattta aagccttttt gactgatact tttttaataa ctacccctat tttatatata
     3061 gttatatacc ttataatggg aagtggtcaa gagtttgcag ataatagaag cttaggttgg
     3121 ggtattattt ttgctgttca ctttatcatt attgctattt tttggctaaa aaatggtcaa
     3181 actccaggat taaaagctta tgatttaaaa cttgtggata gtaaaactga gactagagta
     3241 tctattttac aagcaattat tagatattca gcaacacttt tttctataat ctcaatattc
     3301 tttgtattta ctccctttgt aaacaaagag aaaaaaacat tccaagacat agtttcaaat
     3361 acaaaaataa tagaacaata aatgctattt tttaatattt cagcatttta ctttttttac
     3421 tttgctgctg ttggtgtata tgtaatattt ttaccaaaag tactaaatga tattggatat
     3481 agtgcttttg atattggtat tatattagct gttgctcctt taatgaagtt tgctatgcca
     3541 tttttatttt taaaacatat aaatttaaac aaatcaatgt ttaaaaaagc tctattttta
     3601 acagttttag ctgttactct attttatata acaattgaac atttttatat ttttatgtta
     3661 aacaatgcct ttttagctgc ttgtttatct ttgattttac catatttaga agtaaccact
     3721 gtaactgttt taggaaaaga gagatacgga aaagctagac tttttggctc tattgggttt
     3781 atgcttattg ccttagtttt agcaaaattc ttgacatctc catttatagc tcttcactat
     3841 tatttagcaa ttgtggtatt aactgtaata tttgcgctgc ttcttttgaa aaatgattta
     3901 gaggctaaaa ttaatcaaac aggagatgaa gttttttcat ttttaaaaca ttgggcattt
     3961 tgggctagta tattttttat gcaagttagc tttggtggat tttacaactt ttttactatc
     4021 tacgaactag agtatggatt atctttagag actatatctt atctttgggc ttttggtgta
     4081 atatgtgaga ttttaatgtt atattttcaa gcacctattt taaaaaataa tcttttaaca
     4141 atcataaagt tttgtgtagg aataactata tttaggtggt tacttttata tttataccct
     4201 gataatttag aaatagtata ttttactcaa gctattcatg cattttcatt tgcacttttt
     4261 catagtgctg ttgtaattta tctatactca ctttatgaca ataaaaaact agctcaacag
     4321 tttatgtttg gtgttggtta cggtttaggt ggatttgtag gtgcatttct tgcaggtgct
     4381 acttatggag agtatctatt tttatttagt gctatttttg ccttaatctc ttttatcttt
     4441 gtttggtttt tagataaaaa agataaagtt attttaaaat agttctaaat tataattaat
     4501 aaaatatatt tattcaaatc cacttatttt tcttaaaaat aaaaaactga aaacctacta
     4561 tgcaaagtaa aatagctata aaaatataaa atgcattttc atgatttgct ccaggaattc
     4621 caccaacatt tatacctaaa agtcctgtta aaaaagtaag tggcaagaaa attgcagata
     4681 aaatggctaa tatatacatc tttttattca tctgctctgt taaactattt acaagctctt
     4741 cttgaaaaag tataacttta tctcttatag tatcaagttc ttcaatatgc ctgattaact
     4801 gatccgttgt ctctcttaat tcaatttttt gataatcatc tatccaagat aatttttcat
     4861 tataaagttt aactaatgcc tctttttgcg gagttaaata cctttttaaa ataatactct
     4921 ctcttctaat ttttaatatt tctgttcgaa atgcagtatt atgttcagat tctataatat
     4981 tttcttctaa aaaatctgct ctatcttcta ttttatctat tacattgtcc attctatcaa
     5041 tcattctata agttagttca accaaaaatt cagaagagct ttttacacct tttccttttt
     5101 ttaaattttc acttatttct attacagata gaatattttt tctacttgtt gaaataatca
     5161 catttgaaga gataaaaata cgaatagata tcatcttttc tggttttgaa tttggttcta
     5221 aatttacacc tcttaaagca ataagtaacg catcatttaa aacaatagtt cttggtcttg
     5281 tttcatctgt taaaagtgct tcaactgcaa cagaatcaat attactacta tttctaatcc
     5341 aatcgtttgc ttctttactc gtatagtcaa aatgcaacca caaaatttta ttatctttat
     5401 ctacactctc tatatcttta tatttaagct cttttgctgt tccatctttt tctattaaaa
     5461 agccttgaaa tggtttcatt ttttaccttt atattatgct ataaacaatt tctgttttta
     5521 atttttgctc tttgatttct tcaagtttta gatttttcaa ctccaaattc ataaaatcat
     5581 caaaagtttt tatgtcattt tgtgcaataa tttttaaaat ttctccccta taagcctttg
     5641 caaaatgact tacaactttg ccatctttta taaacttcaa tgttttatat ggttttttta
     5701 taacataaaa cttctcataa aatccagctc ttaaatccaa aatatcatca ttttccaaat
     5761 atttatcaag ttctttacta aaattatcat tataaaattt atctattttt aaatcataaa
     5821 aactttcacc ttgctttagt ttgtaatctg gtatctcatc gcttgctttt agcactccaa
     5881 aaagatttga aaaaatcaaa acattatcat ctatatattt ttgtgaactt ttttctaaat
     5941 tattgtaaga caaataatca taagcaacac cttcatatct ttgtattgct tttaaaagtg
     6001 ggcttttaaa aatatcttgt ctatattttt ctattacatc gctttttttt gtaccaaaaa
     6061 gtttctctaa ttgcgatatt gaagcagttt gtaaaaactc gttataatag tttattattt
     6121 caactctttt attaaaaagc tgtggaaata taaagctatt ttcatcaaaa atcttttcaa
     6181 ctccaccact attttttgtc tcactaggag aaaataaaat tttcatcgct attccttata
     6241 ttaattgctt ataaaatttt gtattttttt at
//
LOCUS       DAOQAN010000005         6053 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_16178_length_6053_cov_3.449150, whole genome shotgun sequence.
ACCESSION   DAOQAN010000005 DAOQAN010000000
VERSION     DAOQAN010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 6053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6053
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16178_length_6053_cov_3.449150"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(25..525)
                     /locus_tag="PKO10_00160"
     CDS             complement(25..525)
                     /locus_tag="PKO10_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW65605.1"
                     /translation="MLVEIAQYIVINLSVAFILGLILGYVIGKGNKEKFNSIQCSKNN
                     DENTIKKNKFKINPIFNKSASLDFKPMVLSSSNKKDNLKKIKGIDEDIENELFKLGIY
                     HFEQISIWSSKNSEWIENFLNLPNYVRANQWVEQAKILKTGRETNYSQKLLDEENEDN
                     IDLTTK"
     gene            complement(530..1519)
                     /locus_tag="PKO10_00165"
     CDS             complement(530..1519)
                     /locus_tag="PKO10_00165"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNW65606.1"
                     /translation="MSIFMKIFILLILFISLNIVSIYTFSYEDYFTNKKEDIELKDKN
                     GIFQFFGKDFFKKVGYKPSEFVISKNNNLLSLDGTFSSNEVAKQVLDFLKINSIQNIK
                     IVENSEVSKDLLREISELTELLKDNFENGAKLSFDGNILILEGELKDNSHKNLITTVI
                     SKIDKYEIQTNIYEKKEHIVDENKDNVTKNENFEEIDKNNEQKEKILSKDDVQSLVNN
                     IFITDKISFERRSSEPTEKSKVLIENLSNILKQNPKFTIEVGGHTDSRGNKELNKKIS
                     QDRANRVKEILESFGVDKDRIKAVGYGNERPIAQDDENGLSEINRRVEFIIGD"
     gene            complement(1547..2569)
                     /locus_tag="PKO10_00170"
     CDS             complement(1547..2569)
                     /locus_tag="PKO10_00170"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002812741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HNW65607.1"
                     /translation="MERKIIYNPNSKESLNDRRVFGGNSDGMINFTKMKYQWALNLWD
                     TMEANTWFPREVQMTQDAKDYKYLTPAEKRMYDLVLSQLIFMDSLQTNNLMDNINPYI
                     TAPEINACLSRQSYEEANHSKSYAVMVESISDNTDLIYDMWKTDAKLKEKNTYIAEVY
                     KNLSQGELTDEKLLLALFANQILEGLYFYAGFAAIYALGKSGKMLGSSQMIRFIQRDE
                     VTHLLLFQNMINSVRKERPELFTSELEESVRSMFRKAVELEASWGSYITQGQILGFTD
                     AIITQYIQYLADRRLEAVGYKAEYSVKHPIPWVDGYASFNDQRTNFFEGNVVNYSKGS
                     IDFDDF"
     gene            complement(2601..4985)
                     /locus_tag="PKO10_00175"
     CDS             complement(2601..4985)
                     /locus_tag="PKO10_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HNW65608.1"
                     /translation="MTIMIKKRNGRKEVLDITKIQKMTIEATAGLEGVSQSELELDSH
                     IKFIDGMNSSDIQDALIKTAVEKIDIDVPNWTFVAARLFLYDLYHRVGIATYGVKGEA
                     YRHLKEYINFGIEAGRLIPNLANGYDLDDLNSYIDPSRDFLFNYLGIKTLYDRYLIKN
                     SKGEPIELPQQMFMAIAMFLAQNEEKKQEKAKEFYDVISKFEVMLATPTLSNARTNRH
                     QLSSCYIGSSPDNIEGIFDGYKEMALLSKYGGGIGWDWNQIRSLGGSIDGHKSAAGGT
                     VPFLKITNDIAIAVDQLGTRKGAIAVYLEPWHMDIMDFIDLKKNSGEERRRAHDLFPA
                     LWISDLFMERVLEDSHWTLFDPVEVKDLSEYFGEEFTAKYIAYENNDNITKNRIKAKD
                     LWKKILTSYFESGSPFLCFKDSANKANPNQHVGLIRSSNLCTEIFQNTNPNHYKIKVE
                     FEDGTIKTFEEEDLVIVDGGITKKANKVTALDSINGKKIFIVEKQKIDGDTAVCNLAS
                     INLSRINTSEDIKRVVPIAVNMLDNVIDLNFYPLRKVKATNLKSRAIGLGVMGEAEML
                     ASNKISWGSNEHFKKIDEIMEYISYNTILASSNLAIEKGSYPTFDGSNWSKGIMPHDH
                     TPQAVNAIVNKDLFDNSCDWDFLREKVKKDGMRNGYLMAIAPTSSISILVGTTQAIEP
                     VYKRKWFEENLSGLIPVVAPKLSPDTWSFYTPAFEIDQLQVVKAAAIRQKWIDQGQST
                     NIFMSLDKASGKYLHEIYTLAWKLGLKSTYYLRSQSPEAKNDVEDRSMECAGCQ"
     gene            complement(4999..>6053)
                     /gene="purB"
                     /locus_tag="PKO10_00180"
     CDS             complement(4999..>6053)
                     /gene="purB"
                     /locus_tag="PKO10_00180"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012084147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HNW65609.1"
                     /translation="TSSDAVDTGVALQMRDSLQIIIDDVKMLLESIKKRANEHKYTLM
                     VGRSHGIHGEPITFGLTLAVWYDEVKRHLKNLEETMEVIAVGQISGAMGNFAHAPLEL
                     EEYAMAELGLKPEPCSNQVIHRDRYARLATSLALLASSVEKFAVQVRHLQRTEVYEAE
                     EYFAAGQKGSSAMPHKRNPILTENITGLARIIRAYVTPALENVALWHERDISHSSTER
                     FWLPDAFITTDFMLHRMNSVIANLTVMPENMMKNLNLTGGLVFSQRVLLELPLQGVSR
                     EDAYKIVQRNAMKVWQEIQQGKPTTNEKGESLYLQYLLADEELRKSLSEDKIRECFNF
                     DYYTKNVDKIFNRVFN"
BASE COUNT         1865 a         1073 c          703 g         2412 t
ORIGIN      
        1 attttaagga gatttttgaa tttactattt tgtagttaaa tcgatattat cttcattttc
       61 ttcatctaaa agcttttggc tatagtttgt ttctcttcct gtttttagaa tttttgcttg
      121 ctctacccat tgattcgctc gtacatagtt tggaagattt aaaaagtttt ctatccactc
      181 cgaattttta ctagaccaaa ttgatatttg ttcaaaatga taaattccaa gtttaaataa
      241 ctcattttct atatcttcat ctatcccttt tatcttttta agattatctt ttttatttga
      301 tgaacttaaa accataggct taaaatctaa actagcactt ttattaaata tgggatttat
      361 tttaaacttg tttttcttaa tagtgttttc atcgttattt ttactacatt gtatagagtt
      421 aaatttctct ttatttcctt ttccaataac ataaccaaga attaaaccta aaataaatgc
      481 aactgataaa tttataacaa tatattgtgc aatctctact agcatattat taatccccta
      541 taataaattc tactcttctg tttatttctg acaatccatt ttcatcatct tgtgcaattg
      601 gtctttcatt tccatatcca actgctttaa ttctatcttt atcaacacca aagctctcta
      661 aaatctcttt tactctattt gctctatctt gagatatctt tttatttagc tctttatttc
      721 ctcttgaatc tgtgtgacca ccaacttcta tagtaaattt aggattttgt tttaaaatat
      781 tactaagatt ttctataaga acttttgatt tttctgttgg ttctgaactt cgtctttcaa
      841 aagaaatttt atctgttata aaaatattat taacaagaga ttgtacatca tcttttgata
      901 atattttctc tttttgttca ttatttttat ctatttcttc aaaattctca ttttttgtaa
      961 cattatcttt attttcatca actatatgct cttttttttc ataaatattt gtttgtatct
     1021 catatttatc tatttttgag ataacagttg ttattaaatt tttatggcta ttatctttta
     1081 actcaccttc taagattaat atatttccat caaaacttaa ttttgcacca ttttcaaagt
     1141 tatcttttaa aagttcagta agttctgaaa tttctcttaa taaatctttg ctaacttcac
     1201 tattttcgac aattttaata ttttggatac tatttatttt tagaaagtca agaacttgtt
     1261 ttgcaacttc attgcttgaa aatgtaccat caagagataa aagattgttg tttttagaaa
     1321 ttacaaattc agatggtttg taaccaactt ttttgaagaa atctttacca aaaaactgga
     1381 aaataccatt tttatctttt agttctatat cctctttttt atttgtaaaa taatcttcgt
     1441 aactaaaagt atatatagaa actatattta aagatataaa taaaatcagt aaaataaaaa
     1501 tcttcataaa aattgacatt tttgccccta tcttttgtgc taaattttag aaatcatcaa
     1561 aatctatact accttttgag tagtttacta catttccttc aaaaaagttt gttctttggt
     1621 cattaaagct tgcatatcca tctacccaag gaatagggtg ttttacactg tattctgctt
     1681 tatatccaac agcttcaagt cttctatctg ctaaatattg aatatattgt gtgattattg
     1741 catctgtaaa tcctaaaatt tgtccttgtg ttatgtaaga accccaagat gcttcaagtt
     1801 caactgcttt tctaaacata gatcttacac tctcttcaag ttctgaagta aatagctcag
     1861 gtctttcctt tctaacacta tttatcatat tttgaaatag taaaagatgt gtaacttcat
     1921 ctctttgaat aaatctaatc atttgtgaac ttccaagcat tttaccactt ttaccaagtg
     1981 cataaatagc tgcaaaaccc gcataaaagt atagaccttc aagaatttga ttagcaaata
     2041 gcgctaaaag aagtttttca tctgttaatt caccttgaga taagttttta taaacttctg
     2101 caatataagt atttttctcc tttaactttg catcagtttt ccacatatca tagattaaat
     2161 ctgtattatc agagattgat tcaaccatta cagcataaga tttactgtga tttgcctctt
     2221 cgtaagattg tctacttaga caagcattta tttcaggcgc agttatatat ggatttatat
     2281 tatccattaa attatttgtt tgtaaactat ccataaaaat caattgactt aatactaaat
     2341 catacattct tttttcagct ggtgtcagat acttataatc ttttgcatct tgtgtcattt
     2401 gaacttctct aggaaaccaa gtatttgctt ccatagtatc ccaaagattt aatgcccatt
     2461 ggtatttcat tttagtgaag ttaatcattc catcactatt tccaccaaaa actcttctat
     2521 cgtttaaaga ctcttttgaa tttgggttgt atataatttt tctttccatc tattttcctt
     2581 taaaaatgta aatggtgtct ttattgacaa ccagcacact ccatacttct atcttctaca
     2641 tcattttttg cttctggact ttgacttctt aaatagtatg ttgatttaag acctagtttc
     2701 caagctaaag tataaatttc atgtagatat tttccacttg ctttatctaa actcataaag
     2761 atatttgtac tttgtccttg gtcaatccat ttttgtctta ttgctgctgc ttttacaact
     2821 tgaagttgat ctatttcaaa tgcaggagtg taaaaagacc aagtatctgg acttaatttt
     2881 ggggctacta ctggaattaa accactcaag ttctcttcaa accattttct tttataaaca
     2941 ggctcaattg cttgagttgt tccaactaaa atagagatag atgatgttgg agcaatagcc
     3001 attaggtaac catttctcat accatctttt ttaacttttt ctcttaaaaa atcccagtca
     3061 caagagttat caaataaatc tttatttact atagcgttta cagcttgtgg agtgtgatca
     3121 tgaggcataa tacctttgct ccaatttgaa ccatcaaaag tagggtaaga acctttttct
     3181 attgctaaat ttgaacttgc taaaatagta ttatatgaaa tatactccat aatctcatct
     3241 attttcttaa agtgctcatt gcttccccaa gatattttat tgcttgctaa catctcagct
     3301 tcacccataa caccaagacc tattgctctt gattttaggt tagttgcttt tacttttcta
     3361 agtggataga agtttaaatc aattacatta tcaagcatat ttacagctat tggtacaact
     3421 ctttttatat cttcacttgt attgattctt gataaattta tacttgcaag gttacaaaca
     3481 gcagtatctc catctatttt ttgtttttca actataaata tttttttacc atttatacta
     3541 tcaagtgcag ttaccttatt tgcttttttt gtaattccac catcaactat tactaaatct
     3601 tcctcttcaa aagttttgat agtaccatct tcaaactcta cttttatttt atagtgattt
     3661 gggtttgtat tttggaaaat ctctgtacaa agatttgaac ttcttatgag tcctacatgt
     3721 tgatttggat ttgccttgtt tgcactatct ttgaaacata aaaacggact tccactttca
     3781 aaatatgaag ttaaaatctt tttccataaa tcttttgctt ttattctatt ttttgtgata
     3841 ttgtcattgt tttcataagc tatatatttt gcagtaaact cttcaccaaa atattcactt
     3901 aaatctttta cttcaacagg atcaaatagt gtccaatgag aatcctctaa aactctctcc
     3961 atgaatagat cactaatcca aagtgctgga aatagatcat gagctctacg tctttcttct
     4021 ccactatttt tctttaaatc aataaagtcc ataatatcca tatgccaagg ctcaagatat
     4081 acagcaattg caccttttct tgtacctaat tgatcaacag caattgctat atcatttgtg
     4141 atttttaaaa atggaactgt tcctccagca gcacttttat gtccatcaat agaaccacct
     4201 aaacttctaa tttggttcca atcccaacca attccaccac catatttaga taatagtgcc
     4261 atctctttgt aaccatcaaa gataccttca atattatcag gacttgaacc aatataacaa
     4321 gagcttaatt gatgtctatt tgttctagca ttacttaatg ttggagttgc aagcattact
     4381 tcaaatttag agataacatc ataaaactct tttgcttttt cttgtttttt ctcttcgttt
     4441 tgagctaaaa acatagcaat tgccataaac atttgttgtg gtaactctat tggctcacct
     4501 ttagagtttt taattaaata tctatcatat agagttttaa ttcctaaata gttaaataaa
     4561 aagtctctac ttgggtcaat atatgagttt aaatcatcta aatcataacc atttgctaaa
     4621 tttggtatta atcttccagc ctcaattcca aagttaatgt actcttttaa gtgtctatat
     4681 gcttcacctt ttactccata agttgcaatt ccaactctat ggtataaatc atataaaaaa
     4741 agtctagcag caacaaaagt ccagtttgga acatcaatat caatcttttc aacagctgtt
     4801 tttatcaaag catcttgaat atcgcttgaa ttcattccat caataaattt tatatgagag
     4861 tctagttcta attcactttg actaactcct tcaagaccag cagttgcttc tatagtcatt
     4921 ttttggattt ttgtaatatc caaaacctct tttcttccat ttcttttttt aatcattatt
     4981 gtcatttaaa atcccctatt aattaaaaac tctattaaaa atcttatcta catttttagt
     5041 gtaataatca aagttaaaac actctcttat tttatcttcg cttaaagatt ttcttaattc
     5101 ctcatcagct aaaagatatt gaagatataa agactcacct ttttcatttg tagttggttt
     5161 cccttgttgt atctcttgcc aaactttcat agcgtttctt tgaactatct tatatgcatc
     5221 ttctctacta actccttgaa gaggaagttc tagtaaaact ctttgagaaa atacaagtcc
     5281 accagttaaa tttagatttt tcatcatatt ttcaggcata actgtaagat ttgcaataac
     5341 gctattcatt ctatgaagca taaaatctgt tgtaataaat gcatctggta accagaatct
     5401 ctctgttgaa gagtgagaga tatctctttc gtgccataaa gcaacatttt ctaaagctgg
     5461 tgttacataa gctctaatta ttcttgcaag tccagttata ttttcagtta aaatagggtt
     5521 tcttttgtgt ggcatagctg aacttccctt ttgtccagct gcaaaatact cttcagcctc
     5581 ataaacttca gttctttgaa gatgtctaac ttgtactgca aatttttcaa cactagaagc
     5641 aagaagtgct aaagaggtag ccaatcttgc atatctatct ctatgaataa cttgattaga
     5701 acaaggttca ggtttaagac caagctcagc cattgcgtac tcttcaagct ctaaaggtgc
     5761 atgagcaaaa tttcccatag ctccagaaat ttgaccaact gcaataacct ccatagtctc
     5821 ttcaagattt tttagatgtc ttttaacttc atcataccaa acagctaaag taagaccaaa
     5881 agttattggc tctccatgaa taccgtggct tcttccaacc ataagagtat atttatgttc
     5941 atttgctctt tttttaattg attcaagtaa catttttaca tcatcaatta taatttgtaa
     6001 agagtctctc atttgtaaag caacacctgt atcaacggca tctgaagatg tca
//
LOCUS       DAOQAN010000006         5996 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_16396_length_5996_cov_2.407002, whole genome shotgun sequence.
ACCESSION   DAOQAN010000006 DAOQAN010000000
VERSION     DAOQAN010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 5996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5996
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16396_length_5996_cov_2.407002"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..135
                     /locus_tag="PKO10_00185"
     CDS             <1..135
                     /locus_tag="PKO10_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002849734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HNW65610.1"
                     /translation="HLPKSTLIMLVASFIGLEKTLELYNIAIKENYRFYSYGDGMLII
                     "
     gene            146..886
                     /locus_tag="PKO10_00190"
     CDS             146..886
                     /locus_tag="PKO10_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNW65611.1"
                     /translation="MAYYVLFDTETTGASQEDRVIQFGSLIVDQRGNIEVFDELCSSD
                     VPIKLEAMEVHNITPNLLENKPKAVDTKFYKRLQELNSSENFLIAHNINFDLDMIKKE
                     GFINNFQLIDTLRCAKHLFSELRYHRLQYLRYALELFKQEESEAKKYNITIKAHDAIG
                     DVLVMKLFLSKLVAKVKEIFPEVNPMKKLVELTSTPVFVQTFKFGKYKGENIEDVAKK
                     DANYLNWMIKNMELDEDMQYTLNMVLKN"
     gene            983..1738
                     /locus_tag="PKO10_00195"
     CDS             983..1738
                     /locus_tag="PKO10_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HNW65612.1"
                     /translation="MELIVLAVVIAILFIIGKNYKTEEFKNINLKQKEIFRGDLLNHE
                     AGLLVALMAKVAKADGKVGELEAEIIKHTFTDISTHFQNSQEVRENLKKLYDQEKDSF
                     ANLITICERLYSLTKTDYSKRLKYMEYLLNLSFIDGDFSKQEQEITEDIAQALKIEQK
                     DYINLISNFENFYKNRANEKALSLEKAYEILESNPNDDDSTLKKNYRNLVKKYHPDII
                     TGQGASQNIIDEATTKLQEINEAYEIIKKQRGI"
     gene            1738..1968
                     /locus_tag="PKO10_00200"
     CDS             1738..1968
                     /locus_tag="PKO10_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014474288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNW65613.1"
                     /translation="MARNFPHSFIVCDCKQVSLGELIYAIKEKGAKTLEDLEDLTDAG
                     SSCGCCKCGETDIGEQKMELYLDEILKKFNKE"
     gene            1972..2193
                     /locus_tag="PKO10_00205"
     CDS             1972..2193
                     /locus_tag="PKO10_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW65614.1"
                     /translation="MIDIKEELIKEIKELLKTDENENIDINPNYLNYFDEDELKDIVF
                     RLIEKKVEQKEGNSAYLDEIYQKTKKDDI"
     gene            2214..2966
                     /locus_tag="PKO10_00210"
     CDS             2214..2966
                     /locus_tag="PKO10_00210"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HNW65615.1"
                     /translation="MNKIKNIKQNLMSFLKEEVLKTGLKKVTLGLSGGLDSAVVAVLC
                     KEVFDDNLNCVLMPSQYSSKSSVEDAIELCKKFDIKHEIVSIEPMLSAYLKNMDENSL
                     RIGNFSARLRMSVLYDISARENSLVVGTSNKSELLLGYGTIFGDLACAINPIGDIYKS
                     DLFELAKYLGVNDSIVKKAPSADFYEGQSDESDLGYSYAKIDSLLKQMIDENRSKDEL
                     LALGFEDEFIETIKKRVKINEFKRRLPIVAKI"
     gene            2981..4117
                     /locus_tag="PKO10_00215"
     CDS             2981..4117
                     /locus_tag="PKO10_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNW65616.1"
                     /translation="MRNIAIYKATLDNEEIDQLKSVLQAKDDLSKVLEFEEKMVKYIG
                     AKYAIATATSTAALHLALSSMRLKRGDKVLMSVNSFVNLPETVRHFDAEPIFVDINLE
                     DMNLDIEKFEEAIIANKSKKLRAAIITFIGGLAPNLDKIYEIGKKYNILIIEDCRAAL
                     GTTYKGKKVGNLGADMTIFSTNPSPSRYAISRSGVLVTNNEELATRAKLLRNHAITTA
                     YDSYGNLDYVYDVDDIGHKFDISELDAAYAIAQLKKTDSFIKRRQEIAKLYEDRLKDI
                     KHITILPHKDEHIYTQFIIKISRNRDAFARALKEKGVSTALNYIPLHLLTYYKNKYSI
                     KITAFPNALNNYQQILSLPIYAGLTNEEVNYVCDQVVQIAQEWI"
     gene            4122..5060
                     /locus_tag="PKO10_00220"
     CDS             4122..5060
                     /locus_tag="PKO10_00220"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014474284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HNW65617.1"
                     /translation="MKQKIIFWIEDYLFFPNSFQKILSYLLLPFSFLYIFIIFIKRVT
                     SSKVDFGIPIISIGNLTVGGSGKTPITIELAKNIKDVCVILRGYGRASKGLFVVSLNG
                     KIKVDVNISGDEAMLLASSLKNATVIVSEDRVLGIKKAKELGSKVIFLDDGFSKYNIK
                     KFDILLKPKDEPTNNFCIPSGAYREPKSFYKKANLVLKEGIDFKREVEIKKDGEKSKL
                     PEKTILLTAISKPKRLLEFLPKNIETIFFEDHHTFTKDDIDTILKKYKDFGIVTTQKD
                     FVKLEKFHLKNIYIMDLKIKISEKLDLKELNTYLKI"
     gene            5121..>5996
                     /locus_tag="PKO10_00225"
     CDS             5121..>5996
                     /locus_tag="PKO10_00225"
                     /inference="COORDINATES: protein motif:HMM:NF016235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF475 domain-containing protein"
                     /protein_id="HNW65618.1"
                     /translation="MKQKPLISYFYSFFAVWFVASVLLFLYGGFFALYQGTILSVLEL
                     SLSFDNAVVNATILATMALVWRRRFLLWGMIIAVFGVRFVFPILIVYFSTSMGFIDSF
                     ELAIKNPQEYEKIIQSSHHVIMSFGGMFLLMLFLKFIFDENKDTHWVKYIESFATKLS
                     KVGDIKALFIMFLMLVITYVAPNEVVMGEQLVTVNKLEILVPMIIGVIAFYLLELLKG
                     VIELKTDSNDSKVTELSGGFISFMYLEVIDMSFSLDGVLGAFAITQNVVIIMLGLGIG
                     AMAVRSLTIFMVEREV"
BASE COUNT         2359 a          594 c          999 g         2044 t
ORIGIN      
        1 catcttccaa aatctacttt gattatgctt gttgcttctt ttattggatt agaaaaaact
       61 ttagagcttt ataatattgc tataaaagag aattatagat tctactctta tggtgatgga
      121 atgttaataa tataaaggat aataattggc ttattatgta ctatttgata cagaaacaac
      181 aggtgcttcg caagaagata gagttattca gtttggaagc ttaattgtag atcaaagagg
      241 aaatattgaa gtttttgatg aactttgtag tagtgatgta ccaataaaac ttgaagctat
      301 ggaagttcac aatatcacac caaatttact agaaaataag ccaaaagccg ttgatactaa
      361 attttataaa agattacaag aactaaatag tagtgaaaat tttttaatag cacacaatat
      421 aaattttgat ttagatatga taaaaaaaga gggttttatt aataattttc aattaataga
      481 tactctaaga tgcgctaaac atctttttag tgagcttcga tatcatagat tacagtatct
      541 aagatacgct ttggaacttt tcaagcaaga agagagtgaa gcaaaaaaat ataatataac
      601 tataaaagct catgatgcaa taggtgatgt attagttatg aagttatttt tatcaaaact
      661 tgtagcaaaa gtaaaagaaa tttttccaga ggtaaatcct atgaaaaaat tagttgaatt
      721 aacttctact ccagtttttg tacagacttt taaatttggg aaatataaag gtgaaaacat
      781 agaagatgtt gctaaaaaag atgcaaacta cctaaactgg atgattaaaa atatggaact
      841 tgatgaagat atgcaatata ctttaaatat ggttttaaaa aattaaatat taataaaaaa
      901 ataattaatt aagctcttta tactctcagt ctaaatttga taaaataaaa actcaaattt
      961 taaaatcaaa aataggatat taatggaatt aatagtttta gctgtcgtaa tagcgatttt
     1021 atttattata ggaaaaaatt ataaaacaga agagtttaaa aatattaatc taaaacaaaa
     1081 agagattttt agaggtgatt tattaaacca tgaagcaggg cttttggttg cacttatggc
     1141 aaaagttgca aaagctgatg gaaaagtagg agagcttgaa gctgagatta taaaacatac
     1201 atttacagat atttcaactc attttcaaaa ctcacaagaa gttcgagaaa atctaaaaaa
     1261 attatatgat caagagaaag atagctttgc aaatcttatt actatttgtg aaagattata
     1321 tagtttaaca aaaacagatt actctaaaag attaaaatat atggagtatt tattaaatct
     1381 ttcttttatt gatggtgatt tttcaaaaca agaacaagag ataactgaag atattgctca
     1441 agctttaaaa attgaacaaa aagattatat aaatcttata tcaaattttg agaattttta
     1501 taaaaataga gcaaatgaaa aagcactatc actagaaaaa gcttatgaaa ttttagaatc
     1561 taatccaaat gatgatgatt caactttaaa gaaaaattat agaaatttag ttaaaaaata
     1621 tcatccagat attattacag ggcagggtgc ttctcaaaat attattgatg aagctacaac
     1681 aaaacttcaa gagataaatg aagcttatga aattattaaa aaacaaagag gtatttaatg
     1741 gcaagaaatt ttcctcactc atttattgtt tgtgattgca aacaagtaag ccttggcgaa
     1801 ttaatatatg caataaagga aaaaggtgca aaaacacttg aagatttaga agatttaaca
     1861 gatgctggaa gttcatgtgg ctgttgtaag tgtggtgaaa ctgatattgg tgagcaaaaa
     1921 atggaactat atttagatga gattttaaaa aagtttaata aagagtaaga gttgatagat
     1981 ataaaagaag agttaataaa agagataaaa gagcttttaa aaacagatga aaatgagaat
     2041 atagatataa atccaaacta cttaaactac tttgatgaag atgaactaaa agatattgta
     2101 tttagattaa tagaaaaaaa agttgaacaa aaagagggaa attcggctta tttggatgag
     2161 atttatcaaa aaacaaagaa agatgatata taatcttaaa ctaaggttta aaaatgaata
     2221 aaataaaaaa tattaagcaa aatttaatga gttttttaaa agaagaagtt ttaaaaactg
     2281 gattaaaaaa agttacttta ggtctttctg gtggacttga ttcagctgtt gtagcagttt
     2341 tatgtaaaga ggtgtttgat gataatttaa attgcgtttt aatgccatca caatactcat
     2401 caaaatcatc tgttgaagat gcgatagagc tttgtaaaaa gtttgatata aagcatgaga
     2461 ttgtttcaat tgaaccaatg ttaagtgcat atctaaaaaa tatggacgaa aatagtctta
     2521 gaattggaaa ttttagtgca agattaagaa tgtcagtttt gtatgatatt agtgcaagag
     2581 aaaactcttt ggtagttgga acttcaaata agagtgaatt acttttagga tatggaacta
     2641 tttttggaga tttggcttgt gcaattaatc caataggaga tatttataaa agtgatctat
     2701 ttgagttagc aaaatattta ggtgtgaatg atagtatagt aaaaaaagca cctagtgctg
     2761 atttttatga aggtcaaagt gatgagtctg atttgggata tagttatgct aaaattgact
     2821 ctttattaaa gcagatgata gatgagaata gaagtaaaga tgagcttttg gctcttggtt
     2881 ttgaagatga atttatagag actataaaaa aaagagtaaa gataaatgag ttcaaaagaa
     2941 gattgccaat agttgctaaa atatagaaat tgaggtaaag atgagaaata ttgcaatata
     3001 taaagcaact ttagataacg aagagattga tcagttaaag tcagttcttc aagctaaaga
     3061 tgatttatca aaagttttag agtttgaaga gaagatggtt aaatatattg gagcaaagta
     3121 tgctattgca acagcaactt caacagctgc tcttcattta gctcttagtt ctatgagatt
     3181 aaaaagaggc gataaggttt taatgtctgt aaactctttt gtaaacttac cagaaactgt
     3241 aagacatttt gatgcagaac ctatatttgt agatataaat ttagaggata tgaatttaga
     3301 tatagagaag tttgaagagg caattattgc aaataaatca aaaaaattaa gagcagcgat
     3361 tatcactttt attggtggac ttgctccaaa tttagacaaa atttatgaaa taggtaaaaa
     3421 atataatatt ttaattattg aagattgtag agctgcgtta ggaactacat ataagggaaa
     3481 aaaagttgga aatttaggtg ctgatatgac aatattttca acaaatccat ctccatctag
     3541 gtatgcaatt agtcgttcag gagttttagt tacaaataat gaagagttag caactagagc
     3601 aaaactttta agaaaccatg caattacaac agcatatgat agttatggaa acttagatta
     3661 tgtttatgat gtagatgata taggtcataa atttgatatt tctgaacttg atgctgctta
     3721 tgcaatagct cagttgaaaa aaacggatag ttttataaaa agaagacaag agatagcaaa
     3781 actatatgaa gatagattaa aagatataaa acatattact atattgcctc ataaagatga
     3841 acatatatac actcaattta taataaaaat atctagaaat agagatgctt ttgcaagagc
     3901 tttaaaagaa aaaggtgttt ctacagcttt aaattatatt cctcttcatc ttttgactta
     3961 ttataaaaat aaatattcta taaaaataac agcatttcct aatgctttaa ataattacca
     4021 acaaatttta tctttaccta tatatgctgg gcttacaaat gaagaggtaa attatgtttg
     4081 tgaccaagtt gtgcaaattg cacaagagtg gatttgaaat tttgaagcaa aaaattattt
     4141 tttggattga agattatctc ttttttccaa attctttcca aaaaatatta tcttatttac
     4201 ttttaccttt tagttttttg tacattttta ttatttttat caaaagagtt acttcatcaa
     4261 aagttgattt tggtattcct ataatatcta ttgggaattt aacagttgga ggaagtggga
     4321 aaacaccaat tacaattgaa ttagcaaaaa atattaaaga tgtttgtgtc attttaagag
     4381 gttatggaag agcttcaaaa ggactttttg tagttagttt aaatggaaaa attaaagttg
     4441 atgtaaatat aagtggtgac gaagctatgc ttttagcatc atctttgaaa aatgcaacag
     4501 taatagttag tgaagataga gttttgggta taaaaaaagc taaagagtta ggttctaagg
     4561 ttattttttt agatgatgga ttttcaaaat acaatattaa aaaatttgat attttattaa
     4621 aaccaaaaga tgaaccaaca aataactttt gtattccaag cggagcctat agagagccaa
     4681 agagttttta taaaaaagcc aatttagttt taaaagaggg tattgatttt aaaagagagg
     4741 tagagataaa aaaagatggt gagaaatcaa aacttccaga aaaaactatt cttttaactg
     4801 caatttcaaa accaaaaagg cttttagaat ttttaccaaa aaatatagag actatctttt
     4861 ttgaagatca tcacactttc acaaaagatg atatagatac tattttaaaa aaatacaaag
     4921 attttggaat tgttacaaca caaaaagatt ttgttaaatt agaaaagttt catttgaaaa
     4981 atatttatat tatggattta aaaattaaaa tatctgaaaa attggatttg aaagaattaa
     5041 atacttattt aaaaatataa atagctaata tttattagat tttgatataa tcgcaaaaat
     5101 ttataaatca agggattata ttgaaacaaa aacctctaat atcttatttt tacagctttt
     5161 ttgctgtttg gtttgtagct tcagtgttac tatttttata tggtggcttt tttgcacttt
     5221 accaaggtac cattttatct gttttggagc ttagtttatc ttttgataat gcagttgtaa
     5281 atgctactat tttggcaaca atggctcttg tttggagaag aagatttctt ctttggggta
     5341 tgattattgc agtatttggg gttagatttg tatttcctat tttaatagta tatttttcaa
     5401 catctatggg atttattgat tcttttgaat tagctattaa aaatcctcaa gagtatgaaa
     5461 aaattattca aagttctcac cacgtaatta tgtcttttgg tggaatgttt ctattaatgt
     5521 tatttttaaa attcattttt gatgaaaaca aagatactca ctgggttaaa tacatagaat
     5581 cttttgctac aaaattatca aaagtaggtg atattaaagc tctatttata atgtttttaa
     5641 tgcttgtaat tacatatgtt gctccaaatg aagtagttat gggagagcaa ttagtaactg
     5701 taaataaatt agaaatttta gttccaatga ttattggtgt aattgctttt tatcttttag
     5761 agcttttgaa gggtgttata gaacttaaaa cagattctaa tgattctaaa gttacagaat
     5821 taagtggagg atttatatct tttatgtatc ttgaagtaat tgatatgagt ttctctttag
     5881 atggagtttt aggagcattt gctattactc aaaatgttgt gataattatg ttaggacttg
     5941 gtattggtgc aatggcagta agaagtctta ctatctttat ggttgaaaga gaggtt
//
LOCUS       DAOQAN010000007         5916 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_16698_length_5916_cov_2.881761, whole genome shotgun sequence.
ACCESSION   DAOQAN010000007 DAOQAN010000000
VERSION     DAOQAN010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 5916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5916
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16698_length_5916_cov_2.881761"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..1074
                     /locus_tag="PKO10_00230"
     CDS             1..1074
                     /locus_tag="PKO10_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008335669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HNW65619.1"
                     /translation="MALSLIVASSLFASEKIFVVERESSSIAVIENDKFKNRMENFRN
                     MNHGVVKFYKDEAYAISRDGYVIKFDPKKEIIEAEYKTSKSAIGFEISEYFVAVANYD
                     DKSVDVLTKDLKPLKKIVTNSRNVGIKLYKNYMIFSQMDSDIISVYKDLNEGKKEPNF
                     VLHKEFKNAGEMPFDAMLQEQSYIMGFFNSAFFGVVDLEKMEYKKIDVFLDEKKQQVL
                     KVPHFGFWSLSKDEIFIPAVGDNKVFVYDKKFKFIKSIDIKGMPVFTSLSPNKDFLAV
                     TFSGEDFPYLQILDTKTFKVIKEYKFDGKVLHVRWSNDKKELYVSVNDSNKIEVLDTI
                     TWKNIKTINDIKKPSGIFIFEER"
     gene            1078..1812
                     /gene="cobA"
                     /locus_tag="PKO10_00235"
     CDS             1078..1812
                     /gene="cobA"
                     /locus_tag="PKO10_00235"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HNW65620.1"
                     /translation="MKKVYLTGAGPGDIELMTLKAARVIKEADVIIYDKLANPEILEM
                     AKDGTEFIFVGKEFGKHLIPQDEINEIIYQASLKYDLVVRLKGGDPFVFGRGGEEALY
                     LKERGVKFEIIPGITSSISVPAYAGIPVTHRGITCSFRVVTGHEAPNKKDTQINWDSF
                     IADETIVFLMGIHNIKLISKKLIKIGKPSTTPCAVISKGTTNEQKVIVGTLEDIADKA
                     KEIPTPAIIVVGEVVKLREELKWFEE"
     gene            complement(1832..2212)
                     /locus_tag="PKO10_00240"
     CDS             complement(1832..2212)
                     /locus_tag="PKO10_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW65621.1"
                     /translation="MDKNKKNILLILLVAIFFVVGFIGFKDSLPENKNERVYGILQEY
                     LPYTIEKRTGGLAIVYKDGREKEKPKNSQIFHITDKLDKEWGKDNLRLDGNILIVLNK
                     DKQELKRVDLNSQELVWVKEFFGI"
     gene            complement(2199..2780)
                     /locus_tag="PKO10_00245"
     CDS             complement(2199..2780)
                     /locus_tag="PKO10_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008335853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HNW65622.1"
                     /translation="MAYFPAFLKFDDKTILIVGGGNIALEKLEHLLNFSSNITLISKN
                     FSEKIDDLIDKHSLKIFQKEYEKGDAKGYDIVIVAVDDFVLQEDIYFETREYKILCNC
                     VDLQQYCDFIFPSYIKRGDLTIAISTSGSSPAFAKNFKIFLSNLIPEGVEDFLKELKE
                     LRSTIPKGKERMRFFDNKVKDYINSWEKKSGQK"
     assembly_gap    2879..2888
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2889..3928
                     /locus_tag="PKO10_00250"
     CDS             <2889..3928
                     /locus_tag="PKO10_00250"
                     /inference="COORDINATES: protein motif:HMM:NF037131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW65623.1"
                     /translation="IGEKNMTQIPQLELKNEVLLRIQKNFPLAKKPFLNIANELHTTE
                     DRVLEILNEAKRDGIIRQTSAIFDTKKLGYKSSLVAFEIEEKDIHNAVKILNSHPGIS
                     HNYERNHSFNIWFTIAVAPNSNLGLEKSIEILAKKTNAKNYIILPTLKLFKISVKLDT
                     TNKEEKQEKLIQKSFTNLDLSESHYKFIKLLQQDIEFKSEPFKFIVDELNISYDELFT
                     TIQEFINSGVMRRFASILNHRKAGFSANAMVVWDIDEKKSQDIGEIAASFSAVSHCYL
                     RPQYPNWKYNLFTMIHGKTEDDTQKTIEDIANKIEYRDKMALYSSKEFKKVRIIYFSD
                     EFENWEKENNN"
     gene            3943..4338
                     /locus_tag="PKO10_00255"
     CDS             3943..4338
                     /locus_tag="PKO10_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW65624.1"
                     /translation="MKLFFELEIAYIFIAIFFLIVTAIVTTRKFSPPNSFKKVFPPVL
                     IFLIIAIFAHFKVTTSRMAEVESEFLKGETVLCENRTKREVSKSIMINDKLGWNLKDN
                     IFSNPEYFKNFHSARCVKMLENMKKEYIK"
     gene            complement(4372..4863)
                     /locus_tag="PKO10_00260"
     CDS             complement(4372..4863)
                     /locus_tag="PKO10_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014473012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW65625.1"
                     /translation="MDEKEKKSFIERVFFKVNSIDKVLESRVDTILLRVINMLKYIFI
                     VIMIIYLAVCLLSLGYKLIAFTIEQGALNFSSIKTILTDGLFTLIVIAIVKTLFIKNG
                     FDYSLTFLEIAFVVIIRKLILLETIPSETSLLVALGITSSIFFILIIYIHNLKRKWQK
                     DAS"
     gene            complement(4927..5862)
                     /gene="hemH"
                     /locus_tag="PKO10_00265"
     CDS             complement(4927..5862)
                     /gene="hemH"
                     /locus_tag="PKO10_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014473383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HNW65626.1"
                     /translation="MENKKRALILLNMGGARDKNELKMFLTNMFNDENILTIKSSFIR
                     KIVASLIVNKRLNEAWKNYELIGNCSPINPLTEKLVEKCNEKIKNFKTYQVMRYTPPF
                     AKDVIENLKKDGIEEVLLLPLYPQYSTTTTKSSLEDFIKFAKNSFKIKSIYDFYRNDK
                     FNSCIVDEIQRIQDGKDDFNLIFSAHGLPQKIVDNGDPYEKQMIEHVEILSQKLKDKN
                     INFKSINLAYQSKVGPLKWLEPSLEDMLKKFKDQKVIIYPLSFIVDNSETTFELDIEY
                     RHISQDLGILDYKVCKCVNYNDNFIDAIKDIIDKN"
BASE COUNT         2273 a          770 c          775 g         2088 t
ORIGIN      
        1 atagctttaa gtctaatagt agctagtagt ttatttgcta gtgaaaaaat atttgtagtt
       61 gaaagagaga gttctagcat agctgttatt gaaaatgata aattcaaaaa tagaatggag
      121 aactttagaa atatgaatca cggagttgtg aagttttata aagatgaagc ttatgcaatt
      181 tcaagagatg gatatgttat aaagtttgac ccaaaaaaag agattataga agctgaatac
      241 aaaactagta aaagtgcaat aggatttgaa atctcagaat actttgtagc tgttgcaaac
      301 tatgatgata aaagtgttga tgttttaaca aaagatttaa aacctctaaa aaaaatagtt
      361 acaaactcac gaaatgtagg gataaaacta tataaaaact atatgatttt ttctcaaatg
      421 gatagtgaca taatatctgt ttataaagat ttaaatgaag gaaaaaaaga gccaaatttt
      481 gttttacaca aagagtttaa aaatgcagga gaaatgcctt ttgatgctat gcttcaagaa
      541 caaagttata taatgggctt ttttaactct gctttttttg gagttgttga tttagagaaa
      601 atggagtaca aaaaaataga tgttttttta gatgaaaaga aacaacaagt tttaaaagta
      661 cctcattttg gtttttggag tttgagtaaa gatgagattt ttataccagc agttggagat
      721 aataaagttt ttgtttatga taaaaaattt aaattcataa aatcaataga tataaaagga
      781 atgcctgttt tcacaagtct tagcccaaat aaagattttt tagcagtaac cttttctgga
      841 gaagattttc catatttaca aattcttgat acaaaaactt ttaaagttat aaaagagtat
      901 aaatttgatg gtaaagtatt gcatgtaaga tggtcaaatg ataaaaaaga actatatgta
      961 agtgtaaatg atagtaataa aatagaggtt ttagacacaa ttacttggaa aaatattaaa
     1021 actataaatg atattaaaaa accttctggt atatttattt ttgaggagag atagagtatg
     1081 aaaaaagtat atttaactgg tgcaggacct ggagatattg agcttatgac tttaaaagca
     1141 gcaagagtta taaaagaggc agatgtgatt atatatgata aacttgcgaa tcctgagata
     1201 ttagagatgg caaaagatgg aacagaattt atatttgtag gtaaagagtt tggaaaacat
     1261 ttaataccac aagatgagat aaatgagata atatatcaag cttctcttaa atacgatctt
     1321 gtagtaagat taaaaggtgg tgaccctttt gtatttggta gaggtggaga agaagctctt
     1381 tatttaaaag agcgtggagt taagtttgaa ataattccag gtattacttc aagcattagc
     1441 gttcctgcat acgctggaat tcctgtaact catagaggaa ttacttgctc ttttagagta
     1501 gtaactggtc atgaagcacc aaacaaaaaa gatactcaaa taaattggga tagttttatt
     1561 gcagatgaaa caatagtatt cttaatggga atccataata taaaattaat atcaaaaaaa
     1621 ctaatcaaaa ttggaaaacc atctacaact ccttgtgctg ttatatcaaa aggtacaaca
     1681 aatgagcaaa aagtaattgt tggaacactt gaagatattg cagataaagc caaagagatt
     1741 ccaactcctg cgataatcgt tgttggagaa gttgtaaaat taagagaaga gttaaagtgg
     1801 tttgaagagt aggaaaccct acttttcaat tttaaattcc aaaaaactct ttaacccaaa
     1861 ctaactcttg actgtttaaa tctactcttt ttagctcttg tttatcttta tttaaaacta
     1921 ttaaaatatt tccatctagt cttaagttat cttttcccca ctctttatca agtttatcag
     1981 ttatatgaaa aatctgagag ttttttggtt tctctttttc tcttccatct ttatatacta
     2041 ttgctaatcc gcctgttctt ttctctattg tatatggaag atactcttgc aaaataccat
     2101 aaactctttc gtttttattt tcaggtaagg aatctttaaa gccaataaaa ccaactacaa
     2161 aaaatatagc aactaataat attaataaaa tatttttctt atttttgtcc actttttttc
     2221 tcccatgaat ttatataatc ttttacttta ttgtcaaaaa atcgcatcct ctcttttcct
     2281 tttggaattg tgcttcttaa ctcttttaac tcttttaaaa aatcttcaac tccttctgga
     2341 attaaattag aaagaaatat tttaaaattt tttgcaaatg caggagagct tccacttgta
     2401 gaaattgcaa tagttagatc ccctctttta atatatgagg ggaaaataaa atcacaatac
     2461 tgctgtaaat ctacacagtt acaaagaatc ttatactctc ttgtctcaaa atagatatcc
     2521 tcttgtagca caaaatcatc tacagcaaca ataactatat cataaccttt agcatcacct
     2581 ttttcatact ctttttgaaa aatcttaaga ctatgtttat ctattaaatc atctattttt
     2641 tcactaaaat tttttgaaat caaagtaata tttgaagaaa aattcaaaag atgttctaac
     2701 ttttctaaag ctatattacc accaccaact atcaaaatag ttttatcatc aaattttaaa
     2761 aaagctggaa aatatgccac taaaaatcct ttttttatat attttattct atcaaagctt
     2821 atgctattct atcttaattt aagtcaatct ataaaaatta ttattaagtt aaaattttnn
     2881 nnnnnnnnaa ataggagaga aaaatatgac acaaattccc caactagaac taaaaaatga
     2941 agttctactt agaatccaaa aaaattttcc acttgcaaaa aaaccttttc ttaatattgc
     3001 aaatgaactt catacaacag aagatagagt tttagaaatt ttaaatgaag caaaaagaga
     3061 tggtattata agacagacat ctgctatttt tgatacaaaa aagctagggt ataaatcatc
     3121 tttagtagca tttgaaattg aagagaaaga tatacataat gctgtaaaaa ttttaaactc
     3181 tcaccctgga atttctcata actatgaaag aaatcactct tttaatattt ggtttactat
     3241 tgctgtggca ccaaattcta atttgggact agaaaaaagt attgaaattt tagctaaaaa
     3301 aacaaatgca aaaaattata taatacttcc aactttaaaa ttatttaaaa tttcagtaaa
     3361 acttgatact acaaataaag aggaaaaaca agagaaatta attcaaaaga gttttacaaa
     3421 tcttgattta tcagagtctc actacaaatt tataaaactt ttacaacaag atatagagtt
     3481 taaaagtgag ccttttaaat ttatagttga tgaactaaat attagctatg atgaactttt
     3541 tacaactatt caagagttta taaactctgg tgttatgcga agatttgcct caattttaaa
     3601 tcatagaaaa gctggtttta gtgcaaatgc tatggttgtt tgggatattg atgaaaaaaa
     3661 atctcaagat ataggagaaa tagcagcatc ttttagtgcg gtaagtcact gttatttaag
     3721 acctcaatat ccaaactgga aatacaatct ttttacaatg attcatggta aaaccgaaga
     3781 tgatactcaa aaaacaatag aagatatagc aaataaaatt gaatatagag acaaaatggc
     3841 actatactca agtaaagagt ttaaaaaagt aagaattatt tactttagtg atgagtttga
     3901 aaactgggaa aaagagaata ataattaaaa atggagtata aaatgaaact tttttttgaa
     3961 cttgaaattg cctatatttt tatagctata ttttttctta tagtaacagc tattgtaacc
     4021 acaagaaaat tttctccacc aaatagcttt aaaaaagttt ttccacctgt tttaatattt
     4081 ctaattattg caatttttgc tcattttaaa gttacaactt caagaatggc tgaagttgaa
     4141 agtgagtttt taaaaggtga aacagttctt tgtgaaaata gaacaaaaag agaagtttca
     4201 aaatcaatta tgataaatga taaattaggt tggaatttaa aagataatat attttcaaat
     4261 cctgaatatt ttaaaaattt tcatagcgca agatgtgtaa aaatgcttga aaatatgaaa
     4321 aaagagtata taaaataagc aacagattgc ttattttatt aaaatgatat tttaactagc
     4381 atctttttgc cactttcttt ttagattatg aatataaatt attagaataa aaaagataga
     4441 ggacgttatt cccaaagcta caagtaaact agtttcacta ggtattgtct caagtaaaat
     4501 aagctttcta attataacaa caaaagctat ttccaaaaat gtcaaagagt aatcaaagcc
     4561 atttttaata aaaagagttt ttacaattgc aattacaatc aaagtaaaaa gaccatctgt
     4621 taatatagtc ttaatagaag agaaattcaa agctccttgc tctatagtaa aagctattaa
     4681 tttatatcct aaactcaaca aacaaactgc taaatatata atcattatta caataaaaat
     4741 atatttaagc atatttataa ctcttaaaag tatagtatct actctacttt ctagaacttt
     4801 atctatacta tttactttaa aaaaaactct ctctataaaa ctctttttct ctttttcatc
     4861 catcttagac cttttgaaca ctcacaaaat ttttttgcga atagtatcca aaaaaaacat
     4921 aaaaaattaa tttttatcta ttatatcttt tatggcatct ataaaattat cattataatt
     4981 tacacattta caaactttat aatctaatat tcctaaatct tgtgaaatat gtctatattc
     5041 aatatcaagt tcaaaagtag tttcagagtt atcaactata aaagatagtg gataaataat
     5101 aactttttga tctttaaatt tttttaacat atcttcaaga gatggctcaa gccacttcaa
     5161 aggaccaact tttgattgat atgcaagatt tattgatttg aagtttatat ttttatcttt
     5221 taatttttga cttaaaatct caacatgctc aatcatctgt ttttcataag gatcaccatt
     5281 atctacaatt ttttgaggta aaccatgggc tgagaatatt agattaaaat catcttttcc
     5341 atcttgaatt ctttggattt catctacaat acaactatta aatttatcat ttctataaaa
     5401 atcatatata gattttattt tgaaactatt ttttgcaaac ttaataaaat cttctaaaga
     5461 tgattttgta gttgttgtag agtattgagg atataaagga agaagtaaaa cttcttctat
     5521 accatctttt tttaaatttt caatcacatc ttttgcaaaa ggtggagtat atcgcataac
     5581 ctgatatgtt ttaaaatttt taattttttc attacatttt tctactaatt tttcagttaa
     5641 aggatttata ggagaacaat ttcctataag ctcgtagttt ttccaagctt catttaatct
     5701 tttatttaca atcaaagaag ctactatttt tcttataaat gaacttttta tagtcaaaat
     5761 attttcatca ttaaacatat ttgttaaaaa catttttaac tcatttttat ctctagctcc
     5821 acccatattt aaaagtatca aagctctttt tttattttcc attttccaac cttattattt
     5881 cattactaca ccaataagcc atatctatat cgtgag
//
LOCUS       DAOQAN010000008         5874 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_16872_length_5874_cov_4.275133, whole genome shotgun sequence.
ACCESSION   DAOQAN010000008 DAOQAN010000000
VERSION     DAOQAN010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 5874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5874
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16872_length_5874_cov_4.275133"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            179..397
                     /locus_tag="PKO10_00270"
     CDS             179..397
                     /locus_tag="PKO10_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW65627.1"
                     /translation="MYQTKKQSFIEANISTAIGFIVSYILSYTILPLYGVEQSHSVSF
                     QITLIYTIASVFRGYFVRRYFNNKIRFD"
     gene            446..664
                     /locus_tag="PKO10_00275"
     CDS             446..664
                     /locus_tag="PKO10_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW65628.1"
                     /translation="MTLEEQIIELENEISSYTTILINQSGYKRIVGAGNEGATTEFTD
                     PIKLKELRQEARDRLANLKLQKTQGYSR"
     gene            661..2136
                     /locus_tag="PKO10_00280"
     CDS             661..2136
                     /locus_tag="PKO10_00280"
                     /inference="COORDINATES: protein motif:HMM:NF016991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HNW65629.1"
                     /translation="MSVITNLWNLFVGSPKTTHNKAKPDGNVEDFSDFGRKTRTQIAY
                     HNLNGTDIPALIKTAQTGIIGSGISIQSRTKEKEIDNSFEDLIREHSKKRNFEVTERF
                     SRDGFLEMCISEVFRKGGVLVRHRYKSSWRIPYKLEAISIDMIDVSKNDNTTRVKNGL
                     RKNTDGAVVSYFIYKDSTKKESIEVKASEISAYMDYWVDISQYTAVSRISQILPELDE
                     LLDYQKKELEAATERSQSSAFWHTKLYDTVTDAINELYRNAKISNQVSKETFAELTEL
                     QREIMKKISLEGIVPAGGLKAIPADDKITQISSKTDSTYGLFTENLTTKLTASQNRSK
                     VLTYKDMRNTNWATINALASIDESENSAEMRRIVENILDDYLERLLVIGIQTNMINLK
                     WEDYLKNPFKYHNWEILRQSLSVRDEVKIANANRINLENNLITEEEIYAKRGQDYKTV
                     MLKKAQTDIELQQEILKMYKNANLPVPERYKTNQTEGENNA"
     gene            2129..3349
                     /locus_tag="PKO10_00285"
     CDS             2129..3349
                     /locus_tag="PKO10_00285"
                     /inference="COORDINATES: protein motif:HMM:NF013506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HNW65630.1"
                     /translation="MPRGSMIKADVAGQFFAICPMKMEAYMNILNGPEISKAEVGVNV
                     ANNNVAYSVVKNVAIIAIDGAMYKKSFSGACGETVVSYPDIIKAIDKAEADVSIDTIL
                     FRVDTPGGSIAGAEEVRYRIKNSSKKTVTFFENLGASGGMWIFTAAQEIYASPITRLG
                     SIGVVVAYEEEDPETKTKRIELVSKNAPNKRCSLSGDCKEKLQTMIDEIETDFFQALN
                     ENTGFAKEDLIQIFEKGGIIKAEIAKEKGFIKDIRHFSVLLSSLNSNTGVVGMPEANE
                     DNILVNGLAFSQDNFAMLIESKEKLNANILALNTQLTDVNASLEDLQNKLNAKEKTIA
                     GITVRVALANSHGISDANTISQVICASSDEEALKVALDYKEKNAPNINQDESQDQFAA
                     MNAYFEAKKQERGR"
     gene            3351..3698
                     /locus_tag="PKO10_00290"
     CDS             3351..3698
                     /locus_tag="PKO10_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW65631.1"
                     /translation="MPLKTDDLFMQVNDTKSVTLQSGVAYTKGMLVTLQSNGKYTNGT
                     VANPDDPTTFGRQVIGILVDDVNATSADRVGVIATDAVANLNKITFAPGQTLAHIAGT
                     LQAKNLKLEGWNK"
     gene            3698..4783
                     /locus_tag="PKO10_00295"
     CDS             3698..4783
                     /locus_tag="PKO10_00295"
                     /inference="COORDINATES: protein motif:HMM:NF015802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HNW65632.1"
                     /translation="MGATFPISAGLRRAFIGASEVAVSQPRFLLAFFGGFPNAMKSVE
                     TEYIESDLFKTKVRVAKFVNPDAVAKGTEKLTFVEQQIKLPTIKDKRSLTSKEMRSKG
                     FGETIYDSSSNQTRVAAAMDTELVDMDSTIMNRLELMAIESIFDGRMTIVGEGENRII
                     DYSRKTGLSINLGAGNYYGSNGGKPNEDFDDFIALLGENGYTATHVVGRLATMKVLAN
                     HTAIKSEIDNRRIENGNLRFQSMMKEKGAIYYGMYKNCELWGYDGNYVDNAGVSQKAV
                     PAKKIAFIAAETDNTIIPGYAGDMHIDSGALSTESMQVSITADKKVQKIHVGGDISAP
                     TLELYMGQTVAPIMPDADCAVVVQILA"
     gene            4793..5122
                     /locus_tag="PKO10_00300"
     CDS             4793..5122
                     /locus_tag="PKO10_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW65633.1"
                     /translation="MKKAILLETTKVNGAFVAGGTILEIGKDIEKEDFESLKKQGLAT
                     EDLGIEEVQSQVSEKDAKIAELEKQIEDIKTQSKVEQEEISKTISEKDAKIAELEKLV
                     ADKNPKK"
     gene            5215..5388
                     /locus_tag="PKO10_00305"
     CDS             5215..5388
                     /locus_tag="PKO10_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW65634.1"
                     /translation="MRVEFIKEQKVNDKLYKIGEEANFSSSVALEFIAKNIAKESKPK
                     IEKKKNDSKSTTV"
     gene            5363..5692
                     /locus_tag="PKO10_00310"
     CDS             5363..5692
                     /locus_tag="PKO10_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW65635.1"
                     /translation="MTANQLLFNSQKFFVESDLSPNDTIEYIYSDTNEKVTDLHVFIN
                     HITDNEKTTNTNLSKATLANVILANPPKQNDSVKLPNDSRWWKVQEWRRINDMYMIEI
                     TANRSRI"
     gene            5689..>5874
                     /locus_tag="PKO10_00315"
     CDS             5689..>5874
                     /locus_tag="PKO10_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW65636.1"
                     /translation="MKIEITLAGNPAAYLDEFGKYSKLALIEQSSKSGKLGMDSIRNE
                     FRNETTEWSKEYKDGKLT"
BASE COUNT         2518 a          720 c         1054 g         1582 t
ORIGIN      
        1 ataatgaatt ttgggaatgg tgtagagaaa agtcaaaaga aggtcatcaa gtatttatat
       61 ctgaatataa tgcacctgat gactttgaat gtatctggaa aatggaagtt acaaactcta
      121 tgaatacaac caaaacatat aaaccgactg aaaaactatt taagtataaa gaataataat
      181 gtatcaaact aaaaaacaaa gctttataga agcaaacata agtactgcaa taggttttat
      241 agtttcatat atactatctt atactattct acctctttat ggagtagagc agtcacatag
      301 tgtaagtttt caaataactt taatatatac aatagcttct gtttttagag gatatttcgt
      361 gagaagatat ttcaataaca aaataagatt tgactaattt ataaataaaa agctataata
      421 aaagcaaaaa gcaaaaggtc aaacaatgac actagaagag caaataattg aactagaaaa
      481 tgaaatatct tcatatacta ctatcttaat aaatcaaagt ggatataaaa gaatagttgg
      541 tgcaggaaat gaaggtgcaa caacagagtt taccgaccca ataaaactaa aagaactaag
      601 acaagaagca agagatagat tggcaaactt gaaacttcaa aaaacacaag gatatagcag
      661 atgagtgtaa ttacaaactt atggaatcta tttgttggtt caccaaaaac aacacacaac
      721 aaagcaaagc cagatggaaa tgtagaagac ttttctgatt ttggtagaaa aacaagaaca
      781 caaatagcat atcacaatct aaatggtact gatatacctg ctttaattaa aacagctcaa
      841 acaggtatta tcggttctgg aatatcaatc cagtcaagaa caaaagaaaa agaaatagac
      901 aactcttttg aagacctaat aagagaacat tcaaaaaaga gaaactttga agttacggaa
      961 agattttcaa gagatggttt tttggaaatg tgtatatcgg aagtttttag aaaaggtggt
     1021 gttttagtta gacatagata taagtcttct tggagaatac cctacaaact agaagcgata
     1081 agtatagaca tgattgatgt tagcaaaaat gacaacacca ctagagtaaa aaatggactt
     1141 agaaaaaata cagatggagc agttgtaagt tattttatat acaaagattc aacaaaaaaa
     1201 gaatcaatag aagtcaaagc aagtgaaata tcagcatata tggactattg ggtagatata
     1261 tctcaatata cagcagtatc aagaatatct caaatcctac cagagctaga tgagttgctg
     1321 gactatcaga aaaaagaatt agaagctgca acagaaagaa gtcaatcaag tgccttttgg
     1381 catacaaaac tatatgatac agttacagat gcaataaatg aattatatag aaatgcaaaa
     1441 atatcaaatc aagtgagtaa agaaacattt gcagagctaa cagaacttca aagagaaata
     1501 atgaaaaaaa tatctcttga aggaatagtt cctgctggtg gattaaaagc aattcctgct
     1561 gatgataaaa ttacacaaat atctagtaaa acagattcta catatggatt atttactgaa
     1621 aatctaacaa caaagctaac agcatctcaa aataggtcaa aagttttaac atataaagat
     1681 atgagaaata caaactgggc aacaataaat gctttagctt caatagatga gtcagagaat
     1741 tcagctgaaa tgagaagaat agttgaaaat atactagatg attatttgga aagactttta
     1801 gttattggta ttcaaacaaa tatgataaat cttaaatggg aagattatct taaaaatcca
     1861 ttcaaatatc ataattggga aatcttaaga cagtctttaa gtgtaagaga tgaagtgaaa
     1921 atagcaaatg caaatcgtat aaatcttgaa aataatctta taacagaaga agagatatat
     1981 gcaaaaagag gacaagacta taaaacagta atgctcaaaa aagcacaaac agatatagaa
     2041 ttgcaacaag aaatattaaa aatgtataaa aatgcaaatc ttccagttcc agaaagatat
     2101 aaaacaaatc aaacagaagg agaaaataat gcctagagga agtatgataa aagcagatgt
     2161 agcaggacaa ttttttgcta tatgcccaat gaaaatggaa gcatatatga atatattaaa
     2221 tggtccagaa atatcaaaag cagaagtggg tgtaaatgta gcaaataata atgttgcata
     2281 ttcagtagtt aaaaatgtag caattattgc tatagatgga gcaatgtata aaaaaagttt
     2341 tagtggtgct tgtggtgaaa ctgtagtatc ttatcctgat attataaaag caatagataa
     2401 ggcagaagct gatgtgagta ttgacactat cttatttaga gtagatactc caggtggaag
     2461 tattgcaggt gctgaagaag tgagatatag aatcaaaaac tcttctaaaa aaacagtaac
     2521 attttttgaa aatctaggtg cttctggtgg tatgtggatt tttacagcag cacaagagat
     2581 atatgcttct cctattacta gactagggtc gataggtgtt gtagtagcat acgaagaaga
     2641 agatccagaa acaaaaacaa aaagaataga attggtatct aagaatgcac caaacaaaag
     2701 atgctctttg agtggtgatt gtaaagaaaa attacaaaca atgattgatg aaattgaaac
     2761 agattttttc caagcattaa atgaaaatac tggttttgca aaagaagatt tgatacaaat
     2821 atttgaaaaa ggtggtataa taaaagcaga aattgcaaaa gaaaagggtt ttattaaaga
     2881 tataagacat ttttcggtat tattaagcag tttaaattca aacacaggag tagtaggaat
     2941 gccagaagca aacgaagata acattttagt aaatggtttg gctttttcgc aagataactt
     3001 tgcgatgcta atagagtcaa aagaaaagtt aaatgcaaat attttagcat taaatacaca
     3061 actaacagat gtaaatgcta gtttagaaga tttacaaaac aaattaaatg caaaagaaaa
     3121 aactatagct ggaatcacag taagagttgc actagcaaac tctcatggta tttctgatgc
     3181 aaatacaatt agtcaagtaa tttgtgctag ttcagatgaa gaagctttaa aagtagcttt
     3241 agactataaa gaaaaaaatg caccaaacat caatcaagat gaaagtcaag atcaatttgc
     3301 tgcaatgaat gcttactttg aagcaaaaaa acaagaaaga gggagataat atgccattaa
     3361 aaacagatga tttatttatg caagttaatg atactaaatc agtaacatta caatcaggtg
     3421 ttgcatacac taaaggtatg cttgtaactt tacaaagtaa tggaaaatat acaaatggaa
     3481 ctgtagctaa tccagatgat ccaactacat ttggtagaca agtaattggt attttagttg
     3541 atgatgtaaa tgcaacaagt gcagacaggg ttggagttat tgcaacagat gcagtagcaa
     3601 atctaaacaa aattacattt gctcctggac aaactttagc acatattgca ggtactttac
     3661 aagcaaaaaa tttaaaatta gaaggatgga ataaataatg ggagcaacat ttccaatatc
     3721 agcaggttta agaagagcat ttattggtgc ttcagaagta gcagtatctc aaccaagatt
     3781 tttattagca ttttttggtg gttttccaaa tgcaatgaaa tcagtagaaa ctgaatacat
     3841 agaatcagat ttattcaaaa caaaagtaag agttgcaaaa tttgttaatc cagatgcagt
     3901 tgcaaaagga acagaaaaat taacatttgt tgaacaacaa attaaattac caacaatcaa
     3961 agataagaga tcattaactt caaaagagat gagaagcaaa ggttttggtg aaacaatcta
     4021 tgattcaagt tcaaatcaaa caagagttgc agctgcaatg gatacagagc tagttgatat
     4081 ggattcaact atcatgaata gattagagct aatggcaata gaatcaattt ttgatggtag
     4141 aatgacaatc gttggagagg gtgaaaatag aatcattgat tatagtagaa aaacaggact
     4201 atctattaat ttaggagctg gaaattatta tggttcaaat ggtggaaaac ctaacgaaga
     4261 ttttgatgat tttatagcac tgttaggaga aaatggatac acagcaactc atgttgttgg
     4321 aagattagct actatgaaag ttcttgcaaa tcatactgca attaaatctg aaattgacaa
     4381 tagaagaatt gaaaatggaa atttaagatt tcaaagcatg atgaaagaga aaggtgcaat
     4441 ttattatgga atgtataaaa actgcgaact ttggggatat gatggtaact atgtagataa
     4501 tgcaggtgtt agtcaaaaag cagttcctgc taaaaaaata gcatttattg ctgctgaaac
     4561 agataatact ataatccctg gatatgctgg agatatgcac attgattcag gtgctttaag
     4621 tacagagtct atgcaagtat caattactgc ggataaaaaa gttcaaaaaa ttcatgtagg
     4681 tggagatatt tcagcaccaa ctttagagct atatatgggt caaacagttg caccaattat
     4741 gccagatgca gattgtgcag ttgtagttca aatcttagca taaggaaata aaatgaaaaa
     4801 agctatatta ttagaaacaa caaaagttaa tggagctttt gttgctggtg gaacaatttt
     4861 ggaaatcgga aaagacatag aaaaagaaga ttttgaaagt ttaaaaaaac aaggactagc
     4921 tacagaagac cttggaattg aagaggtgca atcgcaagtt tctgaaaaag atgcaaaaat
     4981 tgcagagctt gaaaagcaaa tcgaagatat taaaacgcaa tcaaaagtag agcaagaaga
     5041 aatttcaaaa acaatttctg aaaaagatgc aaaaattgca gagcttgaaa aacttgtagc
     5101 agataaaaat ccaaaaaaat aaaatcaata agatttaaac aaaggctatg tagttttcac
     5161 tatgtagcct tttttagttt atgatataat acataaaaat aaaaaaggaa tactatgaga
     5221 gtagaattta taaaagaaca aaaagtaaat gataagcttt ataaaatagg ggaagaagca
     5281 aatttttcta gttctgtagc cttagaattt attgcaaaaa atattgcaaa agaatctaaa
     5341 ccaaaaatag aaaagaaaaa aaatgacagc aaatcaacta ctgtttaatt ctcaaaagtt
     5401 ttttgtagag agtgatttaa gtccaaatga tacgatagag tatatatatt ctgatacaaa
     5461 cgaaaaagta acagaccttc atgtatttat taatcatata acggataatg aaaaaactac
     5521 aaatacaaac ttatcaaaag caacactagc aaatgtaata ttagcaaatc ctccaaaaca
     5581 aaacgattca gtaaagctac caaatgatag tagatggtgg aaagtacaag aatggcgtag
     5641 aataaatgat atgtatatga tagaaataac tgcaaatagg agtagaatat gaaaatagaa
     5701 ataactctag caggtaatcc agcagcatat ttagatgagt ttggaaaata ctcaaaacta
     5761 gctttaatag aacaatcatc aaaatctggt aagttgggta tggattcaat cagaaatgaa
     5821 tttagaaacg aaactacgga atggtcaaaa gaatataaag atggaaaatt gact
//
LOCUS       DAOQAN010000009         5789 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_17244_length_5789_cov_3.140565, whole genome shotgun sequence.
ACCESSION   DAOQAN010000009 DAOQAN010000000
VERSION     DAOQAN010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 5789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5789
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17244_length_5789_cov_3.140565"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="PKO10_00320"
     CDS             complement(<1..602)
                     /locus_tag="PKO10_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014474991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HNW65637.1"
                     /translation="MFTILPIKGYIDQIDGFFCDGIHAGLKPNGNNDLGFIYTKEACT
                     VAAIFTENRFQAAPLKHFLAYEEGFKTNFVLINSKNANALTGRKGIEDINTLFSKLNF
                     DLINPVMSSTGVIGNRLPIEKLVTGALKFDLTAKSGENLSRAIMTTDAYPKTCLYEVK
                     LEDGSSFKIGAVAKGAGMINPNLATMLCFICTDADVPYKD"
     gene            complement(605..1732)
                     /locus_tag="PKO10_00325"
     CDS             complement(605..1732)
                     /locus_tag="PKO10_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HNW65638.1"
                     /translation="MSIFTKFKKYFKWEYNSNVPQYDLNPIIYSKLKPIRSPLILIQI
                     LMMVGTLAYVYLEDYTIMQAIFQTSYTITNTGFGALNESNFKNETILFTVFLMLAGFM
                     SLIFAVGVVIDVFTNGNLRELLRERRMLYKIARLRRHFVLCYHNEYTAQVAKQFRENQ
                     IPFVVVDSSDDIEAIAKEHGYPYFVKEEPYKEEAFLKSHLSSAKGVITLSKNISDNIT
                     LIASVRLYEKELERNPYLIIANAETHNEKVRLKKLGANKVVATPSLMAKRVSAMAISP
                     DMENILDEFLYKKDSPITMEDILIKENSWIVGKELRELNLRDTLRISIIGITESSGAF
                     VQLPNGDKVINKNSKLLIVGSQKGLLKAKRVLNLTNQPKEL"
     gene            complement(1757..1945)
                     /gene="rpmB"
                     /locus_tag="PKO10_00330"
     CDS             complement(1757..1945)
                     /gene="rpmB"
                     /locus_tag="PKO10_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014474993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HNW65639.1"
                     /translation="MARRCAISGKGPMVGNNVSHAKNRTKRRFLPNIRTVRVTLEDGT
                     TTKLKISAKELRTLKKHS"
     gene            complement(2011..2883)
                     /locus_tag="PKO10_00335"
     CDS             complement(2011..2883)
                     /locus_tag="PKO10_00335"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNW65640.1"
                     /translation="MKQTTVYYKISNTLINNLKHRDDIKILKKPSFLTKLFSKKRYPD
                     IYFHSGELDENSIEFIKNSKFVVTNSFSNLNLILAKTKISHEKIKVIYPSVDIKYQKP
                     KELKEKYKDRFSLTENTKIIFFTAKNFKTSGVKEFLQIVSNLSFIDFKVIIAGAKQQL
                     AALEFTLPKYSKLEPKIILLDESKEKLDELYLISDVFLLPSYNKNIASSVIKAMFCKC
                     VVFSTMNNDAKEIIDVYATMENPNDPSTAFKIDAILFDENELKKIKKQNRNIALEMSL
                     DKNIEKFNDILTKI"
     gene            2971..3486
                     /gene="gmhB"
                     /locus_tag="PKO10_00340"
     CDS             2971..3486
                     /gene="gmhB"
                     /locus_tag="PKO10_00340"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016646806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HNW65641.1"
                     /translation="MQKKIIYLDRDGVINEDFGYVSKIENFKFVNGVFEACKEFLALG
                     YEIIVVTNQSGIGRNYYSEDEFLELTKYMKNEFKKKDIDILNVYYCPHNPDDNCSCRK
                     PKAGMILQSLNDFEINLNNSWLIGDKMSDIECAKNGNIPNRILISEDVVDGKDFLLAK
                     SLLDSVKYIKK"
     gene            3493..4500
                     /gene="rfaD"
                     /locus_tag="PKO10_00345"
     CDS             3493..4500
                     /gene="rfaD"
                     /locus_tag="PKO10_00345"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HNW65642.1"
                     /translation="MKYNNINFNKKTILITGGAGFIGSNLAFYFQKNFPESKIVVLDC
                     FRSGETFSNGNLKSFGHFKNLLGFNGVVISGDINDKKLLKSLENNYKFDYIFHQAAIS
                     DTTAIEQDIMIKTNVNAYEDLLKIAIKHRANMIYASSAATYGDSDRFEVGFEKPNNVY
                     GFSKVMMDNITYEYLKKDLPISIVGLKYFNVYGPKEFFKNKTASMVVQFGHQILKGLT
                     PKLFEGSDKILRDFIYIEDIIQANIKACKPKKSGVYNVGTGNARSFEDIVNILQKELE
                     IDNGKEYIPNPYVGSYQFFTEANILTTKKFLDYEPRFSLEDGIKAYIPEIKRLFAEEV
                     K"
     gene            4497..>5789
                     /gene="rfaE1"
                     /locus_tag="PKO10_00350"
     CDS             4497..>5789
                     /gene="rfaE1"
                     /locus_tag="PKO10_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014469174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HNW65643.1"
                     /translation="MIKLNKKPNILVIGDLMIDHYLWGSCDRISPEAPVQVVNVKKES
                     SVLGGAGNVINNLVTLGSVVDVISVIGNDSVANELKSLLEKIDVPTSNLVVENNRKTS
                     KKSRLIASQQQVLRYDMESIDDINENSHKQIIQTLEKNIDKYSSIILSDYGKGVLTTN
                     LTKEIIKIANKNSIKVLVDPKGKDYSKYKGSYTLTPNKKEAMEATNIDIKDESSLIEA
                     LKSLKTQCELEVSLITLSEQGIAIFDDELTIKPTVAREVYDVTGAGDTVIASIAFALG
                     NNLDIKDSIYFANLAAGVVVGKIGSATTTLDEIYEYEYSLHKSNSTSHIKTFDEIKTL
                     ASKLHSQGKKIVFTNGCFDILHVGHVKYLEVAKSYGDVLILGLNADSSVRKLKGPTRP
                     INTQDDRAYILASLESVDYVVIFEEETPYELIKLIKPHV"
BASE COUNT         2103 a          710 c          822 g         2154 t
ORIGIN      
        1 atatctttat aaggtacatc tgcatctgta cagataaaac aaagcattgt tgctagattt
       61 ggatttatca ttccagcacc ttttgcaact gcccctattt taaatgagct tccatcttca
      121 agtttcactt cataaagaca agttttaggg tatgcatctg ttgtcataat agcacgagat
      181 aaattttctc cactttttgc tgttaaatca aactttaaag ctcctgtaac taatttttca
      241 ataggaagtc tgttaccaat aactcctgtg ctactcataa ctggatttat taaatcaaag
      301 tttaatttag aaaaaagagt atttatatct tcaattcctt ttcttccagt caaagcattt
      361 gcatttttcg agttaattaa cacaaaattt gtcttaaacc cctcttcata agctaaaaaa
      421 tgttttaatg gagctgcttg aaatctattt tctgtaaata ttgcagcaac agtacaagcc
      481 tcttttgtat atataaatcc taaatcatta tttccatttg gtttaagtcc agcatgaatt
      541 ccatcacaaa aaaatccatc tatttggtct atataacctt ttattggtaa aattgtaaac
      601 attattataa ttcctttggt tgatttgtta aatttaatac tctttttgct tttaaaagac
      661 ctttttgtga accaacaatt aaaagtttag agtttttatt gataacttta tctccatttg
      721 gaagctgaac aaaagctcca cttgactcag ttattccaat aatagatatt ctaagagtat
      781 ctcttaagtt taattctcgt aactcttttc caacaatcca agagttctct tttattaaaa
      841 tatcttccat tgttattgga ctatcttttt tatataaaaa ctcatctaaa atattttcca
      901 tatcagggct tattgccata gcacttactc tttttgccat taaagatgga gtcgcaacaa
      961 ctttatttgc tcctagtttt ttaagtctta ctttttcatt gtgagtttca gcatttgcaa
     1021 taattaaata tggatttctc tctaactctt tttcataaag tcttacagat gctataagtg
     1081 taatattatc agagatattt tttgataaag taataactcc tttggcagag cttaagtgag
     1141 attttaaaaa agcttcttct ttatatggtt cctcttttac aaaatatgga tatccatgct
     1201 cttttgcaat agcttcaata tcatcacttg aatcaacaac cacaaaagga atttgatttt
     1261 ctctaaactg ttttgcaact tgtgctgtat attcattgtg ataacacaaa acaaagtgtc
     1321 ttcttaatct tgctattttg taaagcattc ttctctccct taaaagttct cttaaattac
     1381 catttgtaaa aacatcaatt acaactccaa ctgcaaagat taaactcata aatccagcta
     1441 acattaaaaa tacagtaaat aatattgtct cgtttttaaa atttgattcg tttaaagcac
     1501 caaatccagt atttgtaata gtataagatg tttgaaaaat ggcttgcatg atagtatagt
     1561 cttctaaata gacataagct aaagtaccaa ccatcataag aatctgaatt agaatcaaag
     1621 gtgatctaat tggttttaat tttgaatata taataggatt taaatcatat tgagggacat
     1681 tagagttata ttcccatttg aaatattttt tgaattttgt gaaaatgctc atataagcac
     1741 ttattctagt gcttaattat gagtgttttt taagagttct taactctttt gcagaaattt
     1801 ttaacttagt tgttgttcca tcttctaatg taactctaac tgttctaata tttggtaaaa
     1861 atcttctttt agttctgttt ttagcgtgac ttacgttgtt tccaaccatt ggtccttttc
     1921 cagaaattgc acatcttctt gccattttgg tattcctttt ttatagtgaa aaataattca
     1981 gaattctatc ttaaaattct taagttagag ttaaattttt gttaaaatgt cattaaattt
     2041 ttcaatattt ttatccaaac tcatttcaag tgcaatattt ctattttgtt tttttatttt
     2101 tttaagttca ttttcatcaa ataaaatagc atctatctta aaagctgtgc tagggtcatt
     2161 tggattttcc attgtagcat aaacatctat tatctctttt gcatcattat tcatagtaga
     2221 aaaaactaca catttgcaaa acatagcttt tataacactg cttgcaatat ttttgttgta
     2281 acttggaagt aaaaaaacat cactgataag atatagttca tctaatttct ctttgctttc
     2341 atctaataat ataatttttg gttccaactt tgaatatttt ggtaatgtaa attccaaagc
     2401 tgccaactgt tgctttgctc ctgcaataat aactttaaaa tctataaatg aaagatttga
     2461 aactatttgt aaaaactctt ttactccact tgttttgaaa ttttttgctg taaaaaagat
     2521 aatttttgta ttttctgtaa gagaaaatct atctttatat ttttctttta actcttttgg
     2581 tttttgatat tttatatcaa cacttggata aataacttta attttttcat gacttatttt
     2641 tgtttttgct aaaattagat ttagatttga aaatgagttt gtaacaacga atttagagtt
     2701 ttttataaac tctattgaat tttcatctaa ttctccacta tgaaaatata tatctggata
     2761 tctttttttt gaaaataact ttgtgagaaa agatggtttt tttaaaattt ttatatcatc
     2821 tctatgtttt aagttgttta tcaaagtatt gcttatttta tagtatacag ttgtttgttt
     2881 cataatttag ttttcctatt tttaaagttg taattatagt taaaagtgct ttatgatatt
     2941 atttaaactt aaattgtaag gataagttat atgcaaaaaa agattatcta tcttgataga
     3001 gatggagtta taaatgaaga ttttggatat gttagtaaaa tcgagaattt taaatttgta
     3061 aatggagttt ttgaagcttg caaagagttt ttagctcttg gatatgagat tattgttgtt
     3121 acaaatcaat ctggaattgg aagaaactat tatagtgaag atgagttttt agagcttact
     3181 aaatatatga aaaatgagtt taaaaaaaaa gatattgata ttttaaatgt atattattgt
     3241 ccacataatc cagatgataa ttgttcttgc agaaaaccaa aagctggaat gattttacaa
     3301 tctttaaacg attttgaaat aaacttaaat aattcatggc taattggtga taaaatgagt
     3361 gatattgagt gtgcaaaaaa tggtaatata ccaaatagaa ttttaataag tgaagatgta
     3421 gtagatggta aagatttttt gcttgcaaaa tcattactag attcagtaaa atatataaaa
     3481 aagtaggaaa atatgaaata taacaatata aattttaata aaaaaacaat tttaataact
     3541 ggtggagctg gatttatagg ttcaaattta gctttttatt ttcaaaaaaa ctttccagaa
     3601 tcaaaaatag tggttttaga ttgttttaga agtggtgaaa ctttttcaaa tggaaattta
     3661 aaaagttttg gtcattttaa aaatctttta ggatttaatg gagttgttat tagtggagat
     3721 ataaatgata agaaattatt aaaatctcta gaaaataatt ataaatttga ttatattttc
     3781 catcaagcag caatttctga tacaactgca attgagcaag atattatgat taaaacaaat
     3841 gttaatgctt atgaagattt actaaaaatt gcaataaaac atagagcaaa tatgatttat
     3901 gcttcaagtg ctgcaacata tggagacagt gatagatttg aagttggttt tgaaaaacca
     3961 aataatgttt acggtttttc aaaagttatg atggataaca taacttacga atatttaaaa
     4021 aaagatttac caatatctat agttggatta aaatatttta atgtttatgg tccaaaagag
     4081 ttttttaaaa ataaaacagc atcgatggta gtacaatttg gtcaccaaat tctaaaagga
     4141 ctcactccaa aactttttga aggaagtgat aagatattaa gagattttat ttacattgaa
     4201 gatattattc aagcaaatat aaaagcttgt aaacctaaaa aatctggtgt ttataatgtt
     4261 ggaactggaa atgctagaag ttttgaggat attgtaaata ttttacaaaa agagcttgaa
     4321 atagataatg gaaaagagta cataccaaat ccttatgttg gttcttacca attttttaca
     4381 gaagcaaata ttttaacgac taaaaaattt ttagattatg aaccaagatt tagtttagaa
     4441 gatggaataa aagcttatat acctgaaata aaaagattat ttgcagaaga ggtaaaatga
     4501 taaaactaaa taaaaaacca aatattcttg taattggtga tttaatgatt gatcactatt
     4561 tatggggaag ttgcgataga atctctcctg aagctccagt tcaagttgta aatgtaaaaa
     4621 aagagagctc tgttttaggt ggcgctggaa atgttattaa taatttagtg acactaggaa
     4681 gcgtagttga tgtaattagt gtaattggga atgactctgt tgcaaatgaa ttaaaaagtt
     4741 tattagaaaa aattgatgtt ccaacatcaa acttagtggt tgagaataat agaaaaactt
     4801 ctaaaaaaag tcgtcttata gcatctcaac aacaagttct aagatatgat atggaaagta
     4861 ttgatgatat taatgaaaat agtcataaac agattattca aactttagag aaaaatatag
     4921 ataaatatag ttcaataatt ttatcagatt atggaaaagg tgttttaact acaaatttaa
     4981 caaaagagat tataaaaatc gcaaataaaa acagtataaa agttttagtt gatccaaaag
     5041 gaaaagatta cagtaaatat aaaggctcat acactctaac tccaaataaa aaagaggcta
     5101 tggaagctac aaatattgat ataaaagatg aaagttcttt aatagaagct ttaaaaagtt
     5161 taaaaacaca atgtgaatta gaagtctcac taataacttt aagcgaacaa ggtattgcaa
     5221 tttttgatga tgaactaact attaaaccaa cagttgcaag agaagtttat gatgtaactg
     5281 gtgctggaga tacagtaatt gcttcaattg catttgcttt aggaaacaat ttagatataa
     5341 aagattctat atattttgca aatttagcag ctggagttgt tgtaggaaaa attggaagtg
     5401 ctacaacaac acttgatgag atttatgaat atgaatacag tttgcataaa tcaaattcaa
     5461 cttcacatat aaaaacattt gatgagatta aaactttagc ttcaaaacta catagtcaag
     5521 gtaaaaaaat agtatttaca aatggttgct ttgatatttt acatgttgga catgttaaat
     5581 atttagaagt agcaaaaagc tatggtgatg ttttaatttt aggattaaat gcagatagta
     5641 gtgtaagaaa attaaaaggt ccaacaagac caataaatac tcaagatgat agagcttata
     5701 ttttagcttc acttgaaagt gttgattatg tggtgatttt tgaagaagag actccttatg
     5761 aattaataaa actaataaaa cctcatgtt
//
LOCUS       DAOQAN010000010         5766 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_17358_length_5766_cov_3.209420, whole genome shotgun sequence.
ACCESSION   DAOQAN010000010 DAOQAN010000000
VERSION     DAOQAN010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 5766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5766
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17358_length_5766_cov_3.209420"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /gene="argB"
                     /locus_tag="PKO10_00355"
     CDS             complement(<1..413)
                     /gene="argB"
                     /locus_tag="PKO10_00355"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HNW65644.1"
                     /translation="MTKTNPKVQTLIDAIPYFKKFYGKTIVIKYGGSAQTSDDLKEKF
                     AQDIVLLTLLGIKPIVVHGGGARITELLTKLEIPSHFVDGYRVTCKESMRVVEMVLSG
                     EINKNLASLLNHHGAKAIGISGKDSGIIKAVPKDSG"
     gene            493..3222
                     /locus_tag="PKO10_00360"
     CDS             493..3222
                     /locus_tag="PKO10_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RecB-like helicase"
                     /protein_id="HNW65645.1"
                     /translation="MEKYLALKASAGSGKTFALTVRYISLLLKGAKPSEILTLTFTNK
                     AAAEMSQRVFNTLQTLGDDEAYLNEIIKVSNFTKEQILSKKSLLIKLFINDSSSIFTI
                     DKFVNKILREFCGYIGISDDFKIANDDMEVLSYKFLQSLDENGFKELVEFSLYEKKKF
                     NSIFELFKILIEKNENINPVEIDAKLINLIKDDILQKAFKIKEHILNCPNASNSAIKA
                     VSFTNFDELFANTWIEKESMYDYSYFKKCANEEINIVFLELRDSLLNYYKLRTSYSLN
                     KIFKLYINFKEFKKSFNIEKNYFEFNDISNLVYELLSNKINKDFLYFRLDSNYNHILI
                     DEFQDTSILQYKILKPLIDEVIAGDSEKFKTFFYVGDPKQSIYRFRGGKRELFDFVLS
                     ENSNIKLEDLNTNYRSSKNIIDFVNDTFLNLSNYDYLAQKSIRKDGFVEVIEDPNLQS
                     DEKFISIFNKINELLNSGINENDIAILCYTNSDVLELFYYLKENLPNLKIRTDMSSKL
                     INQQNVKALINAIKYIYFKEDIYRENFNALVGKDINSEFLLDIDLNELSVEKVIYFIA
                     TYFDIMDENIIKLIEQSNTYFNIVDFIYEIDKLDISIENSENSGLQILTIFKSKGLEF
                     HTTILIDRIKRKNHDKSSLLFDYENINLENIYYKVSGYENFDENYKKALEKEKNLNLD
                     DEKNVLYVALTRAKNNLIVFKKEKSSVFDILNIKAFKFGNLILSEAIQIKNSNKKIAY
                     EALNLGKQDIKSSKDNIVNSDILKSKYFGLATHYTLEMMNNFDEKALNHSLNLSKAKY
                     FNYLDELDFVSIKKIIQNLIRNDKFQNLIKDSQIVQEQALIYNEEIKVLDLLLYKNDR
                     FIIVDYKTTTEVLYSHKTQVEYYKKAVSEIFNIKNVDGYLVYLKEDSIEFFEV"
     gene            complement(3219..3782)
                     /locus_tag="PKO10_00365"
     CDS             complement(3219..3782)
                     /locus_tag="PKO10_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014469334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HNW65646.1"
                     /translation="MRNIKSYLITDPNYFSDNKEIFKEKLIAVLKNHKIDFACFRDKS
                     SKNIEELAKIFLQICKEFKIENILINSNISLAIKLGFDGVHLNSQQFDQIENAKKSNL
                     ITIISCHNLEELDKAMNKNIDFVTYSPIFDTPNKGQSKGVDKLNEVIEKFPNLKIFAL
                     GGIIQNEQIKEIEKTKAYGFASIRYFI"
     gene            complement(3855..4541)
                     /locus_tag="PKO10_00370"
     CDS             complement(3855..4541)
                     /locus_tag="PKO10_00370"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HNW65647.1"
                     /translation="MEGRLFTFLGTIGGHGQEWIILSHFVLVIGIIFLVSRMATRKMQ
                     LVPTGSQNVMEAFVGGVIAMGSDTMGEKNARIYMPLIGSLALVIFVSNMIGVIPGFEA
                     PTSNINFTASLAIIVFVYYNYLGIKKNGFVNYFKHFMGPMPVLAPLMFPIEIISHFSR
                     IISLSFRLFGSIRGDDMFLMVLLMLVPWILPLPGFFLLTAFGVLQAFIFSILTYVYIA
                     GSIMMEEEHH"
     gene            complement(4632..>5766)
                     /gene="ilvA"
                     /locus_tag="PKO10_00375"
     CDS             complement(4632..>5766)
                     /gene="ilvA"
                     /locus_tag="PKO10_00375"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006549 - isoleucine metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HNW65648.1"
                     /translation="KAPILSRELNAEIYLKKENLQLTGSFKLRGAFNRVANLKENQKK
                     NGVVAASAGNHAQGLAFAAQYFKCEATIFMPEATPLTKVLGVKSYGANVVLTGENFDE
                     AYASAIKFAKDNNKEFVHPFADDEVIAGQGTIALEILEKIEDIDQIIVPIGGGGLISG
                     IAIAAKSINPNIKITGVVASGAKGMKESFEARMPIDSSSVRTIADGIAVRDVNPKLLD
                     IILEYVDEIVEVSDNETANAILFLLEKHKLMVEGAGAVSVAAIMHDKVSIENKKVCAV
                     VSGGNIDVTMLSIIIEKGLIKSHRKMNLIVTLMDKPGALMHLTDIFTQCSANIVEIDF
                     DRNSVKLEFGEAYVTIALATKGEEHQEQIRENLKQNGYRFKQI"
BASE COUNT         2155 a          756 c          719 g         2136 t
ORIGIN      
        1 ccactatctt ttggaactgc tttaattatt ccactatctt ttccagatat tccaatagct
       61 tttgcaccat gatgatttaa tagtgaagca agatttttat ttatctctcc acttagaacc
      121 atctcaacaa ctctcatgct ctctttacaa gtaactctgt aaccatctac aaaatgtgaa
      181 ggaatttcaa gttttgttaa aagttctgta attcttgctc ctccaccatg aacaactata
      241 ggttttattc ctaaaagtgt tagcaaaact atatcttgag caaatttttc ttttaaatca
      301 tcgcttgttt gtgcacttcc accatatttt ataactattg tttttccata aaatttttta
      361 aaatatggaa tagcatctat tagagtttga acttttggat ttgtttttgt cataaaaaat
      421 atccttgtaa attttaaagt cttgattgta tctaaaaatc tcttttaaaa tgaaatatag
      481 taaaatgaat ttatggaaaa atatttagca ttaaaagcca gtgcagggag tggaaaaact
      541 tttgcattaa cggtaagata tattagttta cttctaaaag gtgcaaaacc tagcgaaatc
      601 ttaactctaa cttttacaaa taaagcagca gccgagatga gtcaaagagt ttttaataca
      661 cttcaaacat taggtgatga cgaagcatat ttaaatgaga ttataaaagt atcaaatttt
      721 acaaaagagc aaatcttatc taaaaaatca ctacttataa aactatttat aaatgatagt
      781 tcaagtattt ttactattga taagttcgta aataagattt tacgagagtt ttgtggatat
      841 attggaataa gtgatgattt taaaattgca aatgatgata tggaagtttt gagttataaa
      901 tttttacaaa gtttagatga gaatggcttc aaagagttag ttgagttctc tttatatgag
      961 aagaaaaaat ttaactctat ttttgaacta tttaaaatat taattgaaaa aaatgaaaat
     1021 ataaatcctg tagaaataga tgcaaaatta attaatctta taaaagatga tattttgcaa
     1081 aaagctttta agataaaaga acatatttta aactgtccaa atgctagtaa cagcgctata
     1141 aaagctgtat cttttacaaa ttttgatgaa ctttttgcaa atacttggat agaaaaagag
     1201 agtatgtatg attattcata ttttaaaaaa tgtgcaaatg aagagataaa tattgtattt
     1261 ttagaactta gagatagttt gttaaactac tataaattaa gaacttcata tagcttaaac
     1321 aaaatattta aattgtatat aaattttaaa gagtttaaaa aaagttttaa tattgaaaaa
     1381 aactattttg agtttaatga tatttcaaat ttagtttacg aacttttaag taataaaatc
     1441 aataaagatt ttttatattt tagattagat agtaactata accatatttt aatagatgag
     1501 tttcaagata cttcaatttt acaatataaa attcttaaac ccttaattga tgaggtgatt
     1561 gctggagata gtgaaaaatt taaaacattt ttttatgttg gagatccaaa acagagtatt
     1621 tatagatttc gtggaggtaa aagagagctt tttgattttg ttttgagtga aaatagtaat
     1681 ataaaacttg aagatctaaa tacaaactat agatcttcta aaaatattat tgattttgta
     1741 aatgatactt ttttaaatct ttcaaactat gattatttag ctcaaaaaag tattagaaaa
     1801 gatggatttg tagaggttat agaagatcca aatttacaaa gtgatgagaa gtttatatct
     1861 atttttaata aaataaatga gctattaaat agtggtatta atgaaaatga tatcgcaatt
     1921 ttgtgctata caaatagtga tgttttagaa cttttttact atttaaaaga aaacttacca
     1981 aatctaaaaa ttagaacaga tatgagttct aaattaatta atcaacaaaa tgtaaaagca
     2041 cttataaatg ctataaaata tatatatttt aaagaggata tttatagaga aaattttaat
     2101 gctcttgtag gaaaagatat aaatagtgaa tttttgttag atattgattt aaatgagtta
     2161 agtgtagaaa aagttatcta ttttattgca acttattttg atattatgga tgaaaatatt
     2221 ataaaactaa tagagcaatc aaatacttat tttaatatag ttgattttat ttatgagatt
     2281 gataaattag atattagtat tgaaaatagt gagaactctg gcttacaaat acttacaatt
     2341 tttaaatcaa aaggattaga gtttcataca actattttaa ttgatagaat aaaaagaaaa
     2401 aatcatgata aaagttcact tttgtttgat tatgaaaata ttaatttaga aaatatatat
     2461 tataaggttt ctggttatga aaattttgat gagaactata aaaaagcttt ggaaaaagag
     2521 aaaaatctaa atcttgatga tgagaaaaat gttctttatg ttgcgctgac tcgtgcaaaa
     2581 aataatctaa ttgtatttaa aaaagagaaa agtagtgtat ttgatatttt gaatataaaa
     2641 gcttttaaat ttggaaattt aatcttaagt gaagcaatcc agattaaaaa tagtaataaa
     2701 aagattgctt atgaagcatt aaatttaggt aaacaagata taaaatcttc aaaagataat
     2761 attgtaaata gtgatatatt aaaatcaaaa tattttgggc tagctactca ctatacttta
     2821 gaaatgatga ataactttga tgaaaaagct ttaaaccata gtttgaattt atcaaaagca
     2881 aaatatttca actatttaga cgaacttgat tttgtatcca taaaaaagat tattcaaaat
     2941 ttaattagaa atgataaatt tcaaaattta atcaaagatt cacaaatagt tcaagaacaa
     3001 gctttgattt acaatgaaga gataaaagtt ttagacctac ttttatataa aaatgataga
     3061 tttattattg ttgattataa aacgacaaca gaagttttat attctcataa aacacaagtt
     3121 gagtattata aaaaagctgt tagtgaaatt tttaatatta aaaatgtgga tggatattta
     3181 gtttatttaa aagaagatag tatagaattt tttgaagttt aaataaaata tctaatagat
     3241 gcaaaaccgt aagctttagt tttttctatc tcttttatct gctcattttg tattattcca
     3301 ccaagtgcaa atatttttag atttggaaat ttttctataa cttcatttaa tttatctact
     3361 ccttttgatt gacctttgtt tggtgtatca aaaataggtg agtatgttac aaaatctata
     3421 tttttattca ttgccttgtc taactcttct aaattatgac aagatataat tgtgataaga
     3481 tttgattttt ttgcattttc gatttgatca aactgttgtg aatttagatg aactccatca
     3541 aaacctaatt tgattgctaa tgatatattt gagttaatta aaatattttc tattttaaac
     3601 tctttacaaa tttgtaaaaa tatttttgct aactcttcaa tatttttaga acttttatct
     3661 ctaaaacaag cgaaatcaat tttatgattt tttaaaacag ctattaattt ttctttaaaa
     3721 atttctttat tatcagaaaa atagtttgga tctgttatta gataagattt tatatttctc
     3781 atattttctt ccaaataaaa aagggagtaa gcaaaactca ctcccttttt tgatttattt
     3841 atactaagaa cttcttagtg atgttcttct tccatcataa tagatccagc aatataaaca
     3901 tatgttagga tactaaaaat aaatgcttgt aaaacaccaa atgcagttaa taggaaaaac
     3961 cctggtagtg gtaaaatcca tggaactaac attaaaagta ccattaagaa catatcatca
     4021 cctctaattg aaccaaataa cctaaaagat agagatatta ttcttgaaaa gtgtgagata
     4081 atctcaattg ggaacattaa aggagctaac actggcattg gacccataaa atgtttaaaa
     4141 tagtttacaa aaccattttt cttgattcct aaatagttgt aatatacaaa aacaatgata
     4201 gctaaagatg cagtaaagtt aatattactc gtaggtgctt caaaacctgg aattactcct
     4261 atcatgttac taacaaatat aactaaagct aaagaaccaa tcaatggcat ataaattcta
     4321 gcattttttt cacccatagt atcgctaccc atagcaataa caccacctac aaatgcttcc
     4381 ataacatttt gacttcctgt tggaactagt tgcatttttc ttgtagccat tctagaaact
     4441 agaaaaataa ttccaattac taaaacaaag tgagataaaa ttatccactc ttgtccgtga
     4501 ccaccaatag ttcctaagaa tgtaaacaat cttccttcca ttttcttcct tttttctttt
     4561 ctactattac taaattatgc gctgattata atatttgttt tctaaaatct ttataaatat
     4621 aaaaaattaa tctaaatttg tttaaatcta tatccatttt gtttcaaatt ttctcttatt
     4681 tgctcttgat gctcttcacc ctttgttgcc aaagcaatag ttacataagc ttctccaaac
     4741 tctaatttta cagaatttct atcaaaatca atctctacaa tatttgctga acattgagtg
     4801 aaaatatctg ttaagtgcat taaagcacct ggtttatcca ttaaagttac tattaaattc
     4861 atttttctat gagattttat taaacctttt tctattatta ttgaaagcat agtaacatct
     4921 atatttccac cacttacaac cgcacatact tttttatttt caatacttac tttatcgtgc
     4981 ataatagctg caacactaac agcaccagca ccttcaacca tcagtttatg tttttctagt
     5041 aaaaaaagaa ttgcatttgc tgtttcatta tcactaactt caacaatttc atcaacatac
     5101 tccaaaataa tatctagtaa ctttggatta acatcacgaa cggcaattcc atctgcaata
     5161 gttctaacac ttgatgagtc tattggcatt ctcgcttcaa aactctcttt catacccttt
     5221 gcaccactag caactacacc tgtgattttt atatttggat ttatactttt tgcagcaatt
     5281 gcaattcctg agattaatcc tccaccacca attggtacaa ttatctggtc aatatcttca
     5341 attttttcta aaatttctaa agcaatagtt ccttgtcctg ctattacttc atcatctgca
     5401 aatggatgaa caaactcttt gttgttatct tttgcaaatt ttattgcact agcatatgct
     5461 tcatcaaaat tttcaccagt taaaacaacg tttgcaccgt aagatttaac tcctaaaact
     5521 tttgtaagtg gagttgcttc tggcataaaa atagttgctt cacatttaaa atattgagca
     5581 gcgaaggcta aaccttgagc atgatttcca gcacttgcag ccacaacacc atttttcttt
     5641 tgattctctt ttaaatttgc aactctatta aatgctcctc ttaatttaaa acttccagtt
     5701 aattgtaaat tctctttttt cagataaatt tctgcattta actctctact taatatggga
     5761 gctttt
//
LOCUS       DAOQAN010000011         5735 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_17500_length_5735_cov_3.201056, whole genome shotgun sequence.
ACCESSION   DAOQAN010000011 DAOQAN010000000
VERSION     DAOQAN010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 5735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5735
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17500_length_5735_cov_3.201056"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="PKO10_00380"
     CDS             <1..276
                     /locus_tag="PKO10_00380"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510291.1"
                     /note="catalyzes the formation of
                     (R)-2,3-dihydroxy-3-methylbutanoate from
                     (S)-2-hydroxy-2-methyl-3-oxobutanoate in valine and
                     isoleucine biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HNW65649.1"
                     /translation="YSISNTAEYGDYVSGPRVINDESKKAMRQILKEIQNGVFAKDFI
                     LEGQAGYPRMNAERAYTRASLLEQTGVKLRNMMPWIASKKIVNQETN"
     gene            316..1488
                     /locus_tag="PKO10_00385"
     CDS             316..1488
                     /locus_tag="PKO10_00385"
                     /inference="COORDINATES: protein motif:HMM:NF016623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HNW65650.1"
                     /translation="MAKRTRKKRKISPIKKTLRKRNLARNFIVLLALIFILLLFAYFF
                     LSKNNQAVKSSSNNNILQSQKLYTNDEIMEEIIQNLNPDIKNNILEKDLEEEKTKKES
                     FEEELKKDISKEVFERQKEIEKSKIEQKIETKQSKEIEDEIIEEKSQIEKPITKQKKD
                     LITKKDNYKYDLKTKPKLVIIIDDVVSKTQKDKILNIGYPITMAFLPPISGHKESAII
                     AQNLPFHMIHFPMQASKNFKNIEVNTLNISDSYETIEARVKQLRVLYPNATYTNNHTS
                     SVFTENYEAMDKLFRALKKYNFIFVDSRTTPNSVAKELSIKYQMPYIVRDTFLDNDRS
                     FTAIQNQLKDAIRVAKKQGFAIAIGHPYEVTFQVLKESKHLLNDVEPIFLNKLPYL"
     gene            1531..2313
                     /locus_tag="PKO10_00390"
     CDS             1531..2313
                     /locus_tag="PKO10_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013327505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990814 - DNA/DNA annealing activity
                     [Evidence IEA];
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HNW65651.1"
                     /translation="MINQFDFKIKELENMKKYPKELYFIGNTQLLKRKKISIVGTRRP
                     SNYTKEFTYKLASKLSQNGICIVSGAAMGVDSISHSGAGLNNTIAVVANGLDIRYPSV
                     NKNQIIEIEKNGLIISSFKEGEKARLYSFVQRNEIVVALGDKLIVTQADLNSGSMTSV
                     EFAIKQNKEIFVLPHRINESLATNELIKKGLAKAIYDIDEFIEDIVGVGLFGFENSFD
                     EILEYCKSNPIYDEVLLKYPDKILEYELDGKIAIKDGKVFVL"
     gene            complement(2344..3648)
                     /gene="murC"
                     /locus_tag="PKO10_00395"
     CDS             complement(2344..3648)
                     /gene="murC"
                     /locus_tag="PKO10_00395"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014473084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HNW65652.1"
                     /translation="MRVHFIGIGGIGLSALARFLNFDGHSVSGSDMKSSSITKELEKE
                     GIKVSCPQEAKNISDDLDLVIYSAAVTDENPELIEARLKQIRTLSRKEALPIILGDKK
                     NYCVAGAHGKSTTTAILATILQSSALIGAISKEFGSNFRYVDKTVAFEADESDASFLL
                     SNPYCAIVTNAEPEHMEYYHYDYDKFYESYETFLSLAKKRVVNGEDKDIQKLKIEDAT
                     YLYPSKDIKNLCYTLKDGQPCTKFDLKDYGTFEVWGFGFHIASNASLAIMAALNELDI
                     ETIRKNLLNYKGIKKRFDIVQSNEKFVVIDDYAHHPTEIEATMKSVELYDNLTNFNNR
                     IVLWQPHKYSRTSDNLEGFKKCFRRCDELIILPLWTVAGEKKIDIDFEKEFASYNPIF
                     ADRVVSYKGKIELIKDDKIIKTYDEGIFLGVGAGDITYQLRY"
     gene            complement(3650..4237)
                     /locus_tag="PKO10_00400"
     CDS             complement(3650..4237)
                     /locus_tag="PKO10_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW65653.1"
                     /translation="MNKNDLNEARTNPDFLIYLEAAMQNSIKNQNIEMMYEILDTMLV
                     LDLEEEKTNKIYEEILKVATLNLEEKLEKNELLKLENIDFLTIRAFYELAIEKWSNSD
                     FELAKALFFTLANSINDDFLKKNMEVLIVNLSKELDFEDFYEEFVDKDELESKEEYGY
                     FITTFNFDVDDFLKNHQEFLQKEYENLKHLIEKRK"
     gene            complement(4234..5673)
                     /locus_tag="PKO10_00405"
     CDS             complement(4234..5673)
                     /locus_tag="PKO10_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014473081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HNW65654.1"
                     /translation="MKKILIILDGIVAKKLLQRIVESNTGDNNYDVVYMNDVILPIQK
                     PSNFTFYKFDPTSNSKLSMVLDKNIHSEVLMALSSKDEMMSVIKNIREYKKNLQITIL
                     DYWGINIKDPMVSIYKGIDVLANGMVERLPNVPVLAQNIGLKQGEIMEIKIPFGSSYA
                     YRSIDSIEQKDWRIFGLYRNQKLMNLKSSLVLKPNDLILVIGKPTVLMNIYNVIGKTQ
                     GQFPMPFGSKIYLYLDLYLEDEKSVKKALDEAKYLNEKLKNSLLIVKVTRPTTVAIME
                     LIKNELKYFPTIILQIDYANLGFKEIIKEDSRKYNIGLLVLTKSMFKDLYKLNFLLEL
                     KIPIFKMGEESLKASKNVGVVLNDSGSYEQISPIVFDVATQLKLKTKIFDYDPIGQKD
                     NQVSLLDHYENLAKIFNEKLEIIKGVENPIRELKKQKEMLQILPLKQKMFKKRSFIKF
                     FYTNSDLVAFDMNKFNQILIPISEEQVQK"
BASE COUNT         2137 a          825 c          698 g         2075 t
ORIGIN      
        1 tactctattt caaatacagc tgaatatggt gattatgttt ctggacctag agttattaat
       61 gatgagtcta aaaaagctat gagacaaatt cttaaagaga ttcaaaatgg tgtatttgct
      121 aaagatttca tacttgaagg tcaagctgga tatccaagaa tgaatgctga aagagcatat
      181 acaagagctt ctttacttga gcaaacaggt gttaaattaa gaaatatgat gccttggatt
      241 gcatctaaaa aaatagtaaa tcaagagact aactaattat tttaagaggt tctcctctta
      301 aaataaaaat atcttatggc aaaaagaaca agaaaaaaaa gaaaaatttc acctataaaa
      361 aaaaccttaa ggaaaagaaa tcttgcaaga aattttatag tacttttagc tcttattttt
      421 atacttctac tatttgctta ttttttcttg agtaaaaata atcaagcagt aaaatcttct
      481 tctaacaata acatcttaca atctcaaaaa ctatatacaa atgatgaaat tatggaagaa
      541 attattcaaa atctaaatcc tgatataaaa aataatattt tagaaaaaga tttagaagaa
      601 gaaaaaacaa aaaaagagag ttttgaagaa gagctaaaaa aagatatttc caaagaggtt
      661 tttgaaagac aaaaagagat tgaaaaatca aaaattgaac aaaaaatcga aacaaaacag
      721 tcaaaagaga tagaagatga gataattgaa gaaaaatctc aaatagaaaa gcctataaca
      781 aaacaaaaaa aagatttaat aacaaaaaaa gataactaca aatatgattt aaaaactaaa
      841 ccaaaactag tgattataat cgatgatgtt gtatcaaaaa cacaaaaaga taaaatctta
      901 aatattggat atcctataac tatggcattt ttaccaccta ttagtggtca taaagagtct
      961 gcaattattg cccaaaattt accttttcat atgatacact ttccaatgca agcttctaag
     1021 aactttaaaa atattgaagt taatacttta aatatatctg atagttatga aacaatagaa
     1081 gcaagagtaa aacaacttag agttttatat ccaaatgcta cttatacaaa taaccatacg
     1141 agttcagttt ttacagaaaa ttatgaagct atggacaaac tttttcgtgc attaaaaaaa
     1201 tataatttta tatttgtaga tagtagaaca actccaaact ctgtagcaaa agagttatcc
     1261 ataaagtacc aaatgcctta tattgtaaga gatacttttt tggacaatga tagaagcttt
     1321 acagctattc aaaatcagct aaaagatgcg ataagagttg cgaaaaaaca aggttttgca
     1381 atagctattg gacaccctta tgaagttact ttccaagttt taaaagagtc aaaacatctt
     1441 ttaaatgatg ttgagccaat atttttaaac aaacttccat atttataaaa atttagttaa
     1501 aataaatata aaaattatca aggggtgatt atgataaatc aatttgattt taaaatcaaa
     1561 gagcttgaaa atatgaaaaa atacccgaaa gaactatatt ttataggaaa tactcaactt
     1621 ttaaaaagaa aaaaaatctc tatcgtaggt acaagaagac cttcaaatta tacaaaagag
     1681 tttacatata aactagcttc aaaactatct caaaatggaa tttgtatagt aagtggtgca
     1741 gcaatgggag ttgacagcat ttcacatagt ggggctggat taaacaacac aatagcggtt
     1801 gtagcaaatg gtttggatat aagatatcct agtgtaaata aaaatcaaat tattgagatt
     1861 gaaaaaaatg gtttaattat ctcaagtttt aaagagggtg aaaaagcaag attatatagc
     1921 tttgtacaaa gaaatgagat agttgtagct ttaggtgata aattaatagt tactcaagct
     1981 gatttaaact ctggaagtat gacatctgta gagtttgcaa ttaaacaaaa caaagagatt
     2041 tttgttttac cacatagaat aaatgagagt ttagctacaa acgaactaat caaaaaaggc
     2101 ttagcaaaag caatttacga tattgatgag tttatagaag atattgttgg agttgggctt
     2161 tttggatttg aaaacagttt tgatgagatt ttagagtatt gcaaaagtaa cccaatttat
     2221 gatgaagtac ttctaaaata tccagataaa atattagagt atgagttaga tggtaaaata
     2281 gctatcaaag atggaaaagt ttttgtgctt tagtataaat attaatagat ataaatctat
     2341 ctattaatat cttaattgat aagttatatc tccagctcca acacctaaaa atataccctc
     2401 atcataagtt tttatgattt tatcatcttt tataagctct attttacctt tgtatgaaac
     2461 tactctatca gcaaaaattg gattataaga ggcaaactct ttttcaaaat caatatctat
     2521 ttttttctca cctgcaactg tccaaagtgg aagaataatt aactcatcac atcttctaaa
     2581 acacttttta aaaccttcaa gattatcact agttctacta tatttatgtg gttgccaaag
     2641 aactattcta ttgttaaagt ttgtaagatt atcatataac tcaactgatt tcatagttgc
     2701 ttcaatctct gttggatgat gtgcataatc atcaattaca acaaattttt catttgattg
     2761 aacaatatca aatctctttt taataccttt atagtttaaa aggttttttc taatagtctc
     2821 aatatcaagt tcgtttaaag cggccattat tgccaaagag gcatttgatg ctatatgaaa
     2881 accaaatccc caaacttcaa aagtaccata atcttttaaa tcaaattttg tacaaggttg
     2941 cccatctttt agagtgtaac aaagattttt tatatctttt gatggataca gatatgttgc
     3001 atcctcaatt tttaattttt gaatatcttt atcttcacca tttacaactc tcttttttgc
     3061 caaacttaaa aaagtttcat atgattcata aaatttatca taatcatagt gataatactc
     3121 catatgctca ggctcagcat ttgtaactat tgcacaatat ggatttgaaa gtaaaaaaga
     3181 tgcatcgctt tcatcagctt caaaggcaac tgttttatct acatatctaa aattacttcc
     3241 aaactctttt gaaatagcac caattaatgc actactttgt aaaatagttg ctaatattgc
     3301 tgttgtagta gatttaccat gagcacctgc aacacaataa tttttcttat ctcctaaaat
     3361 tataggtagt gcctcttttc ttgaaagagt tctgatttgt ttaagtcttg cttctattaa
     3421 ctcaggattc tcatctgtaa ctgctgctga ataaattact aaatctaaat catcacttat
     3481 attttttgcc tcttgcggac aagatacttt tattccctct ttttctaact cttttgtaat
     3541 tgaagaactt ttcatatcgc ttccacttac gctatgccca tcaaaattca aaaatctagc
     3601 tagtgcagaa agccctattc ctccaattcc tataaaatga actctcaaat tatttccttt
     3661 tttcgattaa atgttttaaa ttttcatact ctttttgtaa aaactcttgg tgatttttca
     3721 aaaaatcatc tacatcaaaa ttaaaagttg taataaaata cccatactct tctttagatt
     3781 caagctcatc tttatcaaca aactcttcat aaaaatcttc aaaatcaagc tcttttgata
     3841 aatttacaat taaaacttcc atattttttt tcaaaaaatc atcattaata ctatttgcaa
     3901 gagtaaaaaa aagtgctttt gctaattcaa aatcactatt tgaccatttc tcaatagcaa
     3961 gttcataaaa agctctgata gttaaaaaat caatattttc tagttttaat aactcatttt
     4021 tttctaattt ttcttcaaga ttaagagtag caacttttaa aatctcttca taaattttgt
     4081 ttgttttttc ttcttctaaa tctaaaacta gcatagtgtc taaaatttca tacatcatct
     4141 ctatattttg attttttatt gaattttgca ttgctgcttc taaatatatt aaaaaatctg
     4201 gatttgttct agcttcgttt aaatcgtttt tattcatttt tgtacctgtt cttcagaaat
     4261 tggaattaag atttgattaa atttattcat atcaaaagca actaaatcac tatttgtata
     4321 gaagaactta ataaatgacc tttttttaaa cattttttgt tttagaggta agatttgtaa
     4381 catctctttt tgttttttta actctcgtat tggattttca acacctttta taatctctag
     4441 tttttcatta aagatttttg ccaaattttc ataatggtct agtaaactta cttgattatc
     4501 tttttgtcct attggatcat aatcaaatat ttttgttttt aattttaact gagtcgcaac
     4561 atcaaaaact attggagaaa tctgttcata acttccacta tcatttagaa caactccaac
     4621 attttttgaa gcttttaaac tctcttctcc cattttaaaa attggaattt ttagctctaa
     4681 aagaaaattt aatttatata aatctttgaa catagatttt gtcaaaacaa gtaaaccaat
     4741 attatatttt ctgctatctt ctttaattat ctctttaaat cctaaattcg cataatctat
     4801 ttgcaaaatt attgtaggaa aatattttaa ttcatttttg ataagttcca taattgctac
     4861 agttgtaggt cttgtaactt ttacaataag taaagagttt tttaattttt catttagata
     4921 ttttgcttca tcaagagctt tttttacact cttttcatct tctaaatata gatccaaata
     4981 taagtaaatt ttacttccaa atggcatagg aaattgtcct tgagtttttc ctataacatt
     5041 ataaatattc atcaaaacag ttggttttcc aataaccaaa atcaaatcat ttggttttaa
     5101 aactaaagat gattttagat tcattaattt ttgatttcta taaagaccaa aaattctcca
     5161 atctttttgt tcaattgagt caatagaacg ataagcataa gagcttccaa atggaatttt
     5221 tatctccatt atttcacctt gctttaatcc aatattttga gctaaaacag gtacatttgg
     5281 taatctttca accattccat ttgctaaaac atcaattcct ttgtaaatac taaccattgg
     5341 gtcttttata tttattcccc aataatctaa aatagtaatt tgaagatttt ttttgtattc
     5401 tctaatattt ttaataacac tcatcatctc atcttttgaa ctaagtgcca ttaaaacttc
     5461 actatggata tttttgtcaa gaaccattga tagttttgag tttgaagtag gatcaaattt
     5521 atagaatgta aaatttgacg gtttttgaat aggtaaaatc acatcattca tatatacaac
     5581 atcatagttg ttatcacctg tatttgactc tacaattctt tgcaaaagct tctttgcaac
     5641 tattccatca agtataatta atatcttttt catgggcgaa attatatctt aaaaagttta
     5701 atagtagatt ttagatagaa tatagtctta attaa
//
LOCUS       DAOQAN010000012         5337 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_19419_length_5337_cov_3.301590, whole genome shotgun sequence.
ACCESSION   DAOQAN010000012 DAOQAN010000000
VERSION     DAOQAN010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 5337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5337
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19419_length_5337_cov_3.301590"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1217)
                     /locus_tag="PKO10_00410"
     CDS             complement(<1..>1217)
                     /locus_tag="PKO10_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014469241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNW65655.1"
                     /translation="TPRFLDNLKNLGFKYATYAGMSISIDDIIIPESKVGHITKSKKD
                     VIEVQKQFSQGLLTEQERYNKTIDIWTETNNKLGSEMMELVKTDKNGFNSIFMMADSG
                     ARGSATQIRQLSGMRGLMAKPDGSIIETPIISNFKEGLNVLEYFISTHGARKGLADTA
                     LKTANAGYLTRKLIDVSQNVRITDEDCGTHEGIEITDITSGNELIESLEERITGRVIA
                     EDIIDPISNEILFTEGTLITEEFARVVKDSEVKSVVIRTPLTCKTENGLCSKCYGLNL
                     GEQRKAKPGEAVGVVAAQSIGEPGTQLTLRTFHVGGTASATQTERELKADKEGFIRYY
                     NIKKYDTQDGKSIVANRRNAGVLLVEPKINAPFKGKVTIEAIHEEVVLTISNGKDKKQ
                     YFLRKSDVAKVNEL"
     assembly_gap    1218..1227
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1228..3103)
                     /locus_tag="PKO10_00415"
     CDS             complement(<1228..3103)
                     /locus_tag="PKO10_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNW65656.1"
                     /translation="MSNNEKVLSPIDIKELERPQDFAAFQLKLASPEKILSWSCGEVK
                     KPETINYRTLKPERDGLFCAKIFGPVKDYECLCGKYKKMRYKGVVCEKCGVEVTSSKV
                     RRHRMGHIELVSPVAHIWMVSSLPSRIGTILGVKLKDLERVLYYEAYIVINSGEAFYD
                     GEKTKKINKYDILNEEQYRTVSDLFEHTGFEAKMGGETIRELLENLDLFELLTLLKDE
                     MQTTKSEAKRKTIIKRLKVVENFINSGNRPEWMMLTQLPVLPPDLRPLVSLDGGKFAV
                     SDVNDLYRRVINRNNRLKRLTELDAPEIIIRNEKRMLQEAVDALFDNGKTANAVKGAN
                     KRPLKSLSEIIKGKQGRFRQNLLGKRVDFSGRSVIVVGPNLNMDQCGIPKKMALELFK
                     PHLMAKLEEKGYATTLKAAKRLIESETNEVWECLNEIVDEYPILLNRAPTLHKLSIQA
                     FHPVLIDGKAIRLHPLVCAAFNADFDGDQMAVHVPLSQEAVAEAKVLMMSSMNILLPA
                     SGRAIAVPSQDMILGIYYLSLVKDGVKGEHKLFTDVNEVKIALDMGQIDLHAKIRTKL
                     EGRVISTTVGRLIIHEILPSFVPMNLWNKVLKKKDIGALVDYIYKEAGSSDSASFFIT
                     I"
     gene            complement(3096..>5337)
                     /gene="rpoB"
                     /locus_tag="PKO10_00420"
     CDS             complement(3096..>5337)
                     /gene="rpoB"
                     /locus_tag="PKO10_00420"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HNW65657.1"
                     /translation="PGSTKVDENGKIIEPLIEVRLNGEIILMDKNKVDLIDISSQMVM
                     GVAASLIPFLEHNDANRALMGSNMMRQAVPLIKPTAPIVGTGLEKTVARDAWEAIKAN
                     RGGVVEKADSKNIYISGEDENGPFIDYYDVSKNVKTNNNTSFGQRVAIKEGDIIEKGQ
                     VIADGPSMDKGELAVGINAMVAFMPWNGYNYEDAIILSERLIKKDAFTSVHIYEKEIE
                     CRELKHGNEEITRDLPGVKEENLSHLDSSGIVKVGTYVTPGMILVGKVTPKGEIKPTP
                     EERLLRAIFGDKAGHVINKSLYCPTSMEGTVVDVKVFTKKGYEKCERAKAEIELEKNE
                     LNQKHLDKLLMLDREEALKINNLLTKSKLDKELELDGVVYKKGDTLTLETLLSVNRFA
                     MKKVVSSYAKEVEKAYNDTKEYFIRQKAELRKDHEEKLNVLEHDDILSSGVIKQVKVY
                     IATKRKIKVGDKMAGRHGNKGIVSNIVPKVDMPYLEDGTTVDIILNPLGVPSRMNIGQ
                     IMEVHLGLVGRRLGKQIQQIYDAKKGEYIADLRAKMIEIAGVAKLMNAKAFIEKLSDD
                     ELLDYARDWSKGVKFATQIFDGVEAYDFEKLFEMAKIDSDGKSVLYDGKTGEKMKERV
                     NVGYMYMLKLHHLVDEKVHARSTGPYSLVTQQPVGGKALFGGQRFGEMEVWALEAYGA
                     TAVLKEMLTTKSDDVDGRTRAYRAIANGENVPSSGVPETFFVLTKELKALALDVEIFE
                     EVENNE"
BASE COUNT         1596 a         1073 c          624 g         2034 t
ORIGIN      
        1 agttcattta cttttgcaac atcacttttt cttaagaaat attgtttttt atcttttcca
       61 ttagaaatag ttaaaacaac ctcttcgtga atagcttcaa ttgttacttt tcctttaaat
      121 ggtgcattta tttttggctc aactaataat actccagcat ttcttctatt tgctacaata
      181 gattttccat cttgtgtatc atatttttta atattatagt atctaataaa cccttcttta
      241 tcagctttta actctctttc agtttgagtt gcacttgcag ttcccccaac gtggaaagtt
      301 ctaagcgtta gctgagttcc tggctcacca attgattgag ctgcaacaac tcctacagct
      361 tctcctggct tagcttttct ttgctcacct agatttaatc cataacattt tgaacaaagt
      421 ccattttcag ttttacaagt aagtggtgtt ctaataacaa cagatttaac ttcactatct
      481 tttacaactc ttgcaaactc ttcagttatt aaagtacctt cagtaaatag tatctcattt
      541 gaaattgggt caattatatc ttcagcaata actcttcctg taattctctc ttctaaagac
      601 tcgattaact catttccaga agtaatatct gtaatttcta taccttcgtg agttccacaa
      661 tcttcatcag taattcttac attttgagaa acatcgatta gttttcttgt caaatatcca
      721 gcatttgctg ttttaagcgc tgtatctgca agaccttttc tagctccgtg agtagaaata
      781 aagtactcta gaacgttaag accctcttta aagttagata taattggtgt ttcaataata
      841 cttccatcag gtttagccat aagacccctc ataccagaca actgtctaat ttgagttgca
      901 ctacctcttg ctcctgaatc tgccatcata aaaattgagt taaatccatt tttatctgtt
      961 ttaactagct ccatcatctc agaacccaat ttattatttg tttctgtcca aatatcaata
     1021 gtcttgttgt atctctcttg ctcagttaaa agtccttgag agaactgttt ttgaacttca
     1081 ataacatctt ttttagattt tgtaatatgc cctactttac tttctggaat aataatatca
     1141 tcaatagata tagacattcc tgcataagtt gcatatttaa aacctaaatt tttaaggtta
     1201 tctaagaatc taggagtnnn nnnnnnngaa tagtaataaa aaaagaagca gaatctgatg
     1261 atcctgcttc tttataaata taatctacca gtgcaccaat atctttcttc ttaagaactt
     1321 tattccataa attcattggt acaaaagatg gaagtatctc atgtatgatt aatcttccaa
     1381 cagttgtaga tataactctt ccttcaagtt ttgttctaat ttttgcatgt aaatcaatct
     1441 gtcccatatc aagagcaatt tttacttcat ttacatcagt gaaaagttta tgttcacctt
     1501 ttacaccatc ttttactaaa gatagataat aaattcctaa aatcatatct tgagaaggaa
     1561 cagcgattgc tctaccactt gctggtaata aaatattcat agaactcatc attaaaactt
     1621 ttgcttcagc aactgcttct tgagaaagtg gtacgtgaac agccatttga tccccatcga
     1681 aatcggcatt aaatgcagca caaacaagag gatgaagtct tattgctttt ccatcaatta
     1741 acacagggtg aaatgcctga atagacaatt tatgaagagt tggtgctcta tttaataaaa
     1801 taggatactc atcaacaatc tcatttaaac actcccaaac ttcatttgtt tcagattcaa
     1861 ttaatctttt tgcagctttt agagttgttg cataaccttt ctcttcaagt tttgccatta
     1921 aatgtggttt aaatagttca agtgccattt ttttaggaat accacattga tccatattta
     1981 aattaggtcc aacaacaata acagatcttc ctgagaagtc aacccttttt ccaagtaagt
     2041 tttgtctaaa tcgtccttgc ttacctttaa taatctcact taaagattta agaggtcttt
     2101 tatttgcacc tttaactgca tttgctgttt ttccattatc aaataatgca tcaacagctt
     2161 cttgaagcat tcttttttca tttctaataa taatttcagg agcatcaagt tcagttaatc
     2221 tttttaatct attatttcta tttataactc ttctataaag gtcatttacg tcagaaacag
     2281 caaattttcc accatcaagt gaaacaagag gtcttaaatc aggtggaaga actggaagtt
     2341 gtgtaagcat catccattca ggtctatttc cactatttat aaagttttca actaccttta
     2401 atctttttat tatagtcttt ctttttgctt cactcttagt tgtttgcatc tcatctttta
     2461 aaagtgttaa aagctcaaat aaatcaagat tttctaaaag ctctctaata gtctctccac
     2521 ccatttttgc ttcaaaacct gtatgctcaa ataaatcaga aactgttcta tactgctctt
     2581 catttaaaat atcatatttg tttatttttt tagttttttc accatcataa aaagcttctc
     2641 cagagttaat tactatatat gcttcataat ataaaactct ctctaaatct tttaatttaa
     2701 cacctaaaat agttccaatt cttgatggaa gagaagatac catccaaata tgagcaactg
     2761 gagatactaa ctcaatatga cccattctgt gtcgtcgcac ctttgaagaa gttacttcaa
     2821 ctccacattt ttcacaaaca acacctttgt atctcatctt tttatattta ccacaaagac
     2881 actcataatc ttttactggt ccaaagattt ttgcacaaaa cagtccatct ctttcaggtt
     2941 ttagtgttct atagtttata gtttcaggtt ttttaacctc accacaagac caagaaagta
     3001 ttttttcagg acttgctaat tttaattgga aagcagcaaa atcttgtggt ctttcaagct
     3061 cttttatatc aattggtgat agtacttttt cattattact cattgttttc tacctcttca
     3121 aaaatctcta catctaaagc taaggctttt aactcttttg ttaaaacaaa gaatgtctct
     3181 ggaactcctg aacttggtac attttcacca tttgcaatag ctctatatgc tcttgttctt
     3241 ccatcaacgt catctgattt agttgttagc atctctttta gtacagcagt tgcaccataa
     3301 gcttctaatg cccaaacctc catctctcca aatctttgtc caccaaatag agcttttcct
     3361 ccaactggtt gttgtgttac aagtgaatat ggacctgtac ttcttgcgtg aactttttca
     3421 tcaactaagt ggtgaagttt cagcatatac atatacccaa cgtttactct ctctttcatc
     3481 ttttcacctg tttttccatc gtataaaaca gatttaccat cgctatctat ttttgccatt
     3541 tcaaacagtt tttcaaagtc ataagcttca acaccatcaa aaatttgtgt tgcaaattta
     3601 acaccttttg accaatctct agcataatca agaagttcat catcacttaa tttttcaata
     3661 aatgcttttg cattcataag ttttgcaact cctgcaattt caatcatttt tgctcttaaa
     3721 tcagcgatat actcaccttt ttttgcatca tagatttgtt gaatttgttt accaagtctt
     3781 ctacctacaa gccctaagtg aacctccata atttgtccaa tattcatcct tgaaggaacc
     3841 ccaagtggat ttagaatgat atcaactgtt gttccatctt ctaaatatgg catatcaact
     3901 tttggaacga tatttgaaac aatacctttg tttccatgtc ttccagccat tttatcacca
     3961 actttaattt ttctcttagt tgcaatataa actttaactt gtttgataac tccacttgat
     4021 aaaatatcat cgtgctctag aacatttaac ttctcttcat gatcttttct taattcagct
     4081 ttttgtctaa taaaatactc ttttgtatca ttataagctt tttcaacctc ttttgcataa
     4141 gaagatacta cttttttcat agcaaatcta tttacactta aaagagtctc aagagttaat
     4201 gtatcacctt ttttgtacac aactccatct aactcaagct ctttatcaag cttcgatttt
     4261 gttagaagat tatttatttt taaagcctct tctctatcta gcattaaaag cttatcaaga
     4321 tgtttttgat ttaactcatt tttttctaat tcaatttcag ccttagctct ttcacacttc
     4381 tcataacctt ttttagtgaa aactttaaca tcaacaactg ttccttccat tgatgttgga
     4441 caatataaag atttatttat aacatgtcct gctttatcac caaaaattgc tctaagtaat
     4501 ctctcttctg gagttggttt aatctcacct tttggtgtta cttttccaac taaaatcatt
     4561 ccaggagtta cataagttcc aacttttaca attcccgagc tatcaagatg tgataaattc
     4621 tcttctttaa cacctggtaa atccctagtt atctcttcat taccatgttt taactctcta
     4681 cactcaatct ctttttcata aatatgtaca gatgtaaatg catctttttt aataagtctt
     4741 tcacttagaa taatagcatc ctcatagttg tatccattcc atggcataaa tgcaaccata
     4801 gcattaattc caactgcaag ctcaccttta tccattgatg gaccatcagc aattacttga
     4861 cctttttcaa taatatcacc ctcttttata gcaacccttt gcccaaaaga tgtattatta
     4921 tttgttttta catttttgct tacatcataa tagtcaataa atggtccatt ttcatcttca
     4981 ccactaatat agatgttttt agaatctgct ttttcaacaa ctccacctct attagcttta
     5041 attgcttccc aagcatctct tgcaactgtt ttttctaaac cagttccaac tattggagct
     5101 gttggtttaa tcaatggaac agcttgtctc atcatatttg aacccattaa tgctctattt
     5161 gcatcattgt gctctaaaaa tggaattaaa gaggctgcaa cacccattac catttgtgat
     5221 gatatatcta ttaaatcaac tttattttta tccatcaaaa taatttcacc attaagtcta
     5281 acttcaatta atggttcaat aatttttcca ttttcatcaa cttttgttga accagga
//
LOCUS       DAOQAN010000013         5336 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_19426_length_5336_cov_3.247680, whole genome shotgun sequence.
ACCESSION   DAOQAN010000013 DAOQAN010000000
VERSION     DAOQAN010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 5336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5336
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19426_length_5336_cov_3.247680"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1600)
                     /locus_tag="PKO10_00425"
     CDS             complement(<1..1600)
                     /locus_tag="PKO10_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HNW65658.1"
                     /translation="MSDYGASNIKVLKGLEAVRKRPGMYIGDTNINGLHHLVYEVVDN
                     SIDEAMAGFCRNIKVTLTKDGWARIEDDGRGIPTAIHPTEGISAATVALTVLHAGGKF
                     DKDTYKVSGGLHGVGVSVVNALSKHLKMTVYREGKIHYQEFKEGIPQGALEVIGESPR
                     KTGTTIEFLVDDSIFEVTKYEFNILKKRFKEVAYLNPIISITLEDELAKIKEVYHFEG
                     GIKQFVADLNKETALCEVMHFSDKVDGVEVDIAMMYNDTYIEKTLSFVNNIRTIDGGT
                     HEAGFKAGLTRSISKYLSENAAAREKDAKITGDDVREGLIAVVSVKVPEPQFEGQTKG
                     KLGSSYVRPIAQKLTSDNLDKYFEENPTHARAVMEKALMAARGREAAKKARELTRKKD
                     SMSVGTLPGKLADCQSKDPTIRELYLVEGDSAGGSAKQGRDRVYQAILPLKGKILNVE
                     KSRLDKILKSDEIRNIITALGCGIGEDFNDEKVRYHKIIVMTDADVDGSHIQTLLLTF
                     FFRFLRPIVEKGYLYIAQPPLYRYK"
     gene            complement(1613..2683)
                     /gene="dnaN"
                     /locus_tag="PKO10_00430"
     CDS             complement(1613..2683)
                     /gene="dnaN"
                     /locus_tag="PKO10_00430"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HNW65659.1"
                     /translation="MKLVINKSVFENVVSQMQPFLEKKDSSAITSHLYLEIANSKMTI
                     KATDYEIGLEVIIDTISNFEDGKTTVNGNNLLGFIKRLKNEDITLETSSNNLIIKQGK
                     SIFKLPSYDPNEYPTINRYENLKDLTISTINFINSIKKISPAIDNNNPKFELNGALLD
                     IKSQKINFCATDTRRLAMNSLENMSNEEVQLIIPKKAIFEIQKLFLDNAKISYDNTNL
                     VISNENTTFFTKLINGKYPDYERIIPSNLKNNLYIPKNIFIESIKLITSLNSNIKITF
                     TKQAIIFESLDTDSVANTQVDIDLNIPNDFYIAVNAKYILDFLSMTTSDKIKIGFNES
                     NLPFYLEDNKFITIVMPIVLEK"
     gene            complement(2831..4144)
                     /gene="dnaA"
                     /locus_tag="PKO10_00435"
     CDS             complement(2831..4144)
                     /gene="dnaA"
                     /locus_tag="PKO10_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014472717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HNW65660.1"
                     /translation="MTNKEFLSLIQKETSKNDFERYLKQLVLKKLSIEENIAIFEVGN
                     KYIASWIKSKYTNLIQNCFEKNINIKPDIEIRLIGEKKTKKETQNIQIQNHTPESTIL
                     NPSYTFDSFIVGPSNQMAYNAALAVASKPGVQYNPLFIYGGTGLGKTHILQAIGNHAL
                     ENDKTVIYVTIEQFMNDFIFSINKKNMEHFREKYRNCDLLLIDDVQFLSGKESTQEEF
                     FHTFNELHNAKKQIVMTSDRLPSQIAGLVDRLKSRFEWGLTTDVQIPKLETKIAIIEK
                     KSELNGINLSRDIVSFIATTLDSSIREIEGVLIRINASASLLNLEINLQMVQNLLKDQ
                     IKENKENIKLPDIISLVANELNIKPSDIKSKKRTSSVANARRIVIYLARELTHNSMPD
                     IAKFLEMKDHSSISHNVKKTTELIEKDENFKLIIQNLKNKLINKE"
     gene            4377..4847
                     /gene="ruvC"
                     /locus_tag="PKO10_00440"
     CDS             4377..4847
                     /gene="ruvC"
                     /locus_tag="PKO10_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006802318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HNW65661.1"
                     /translation="MKILGIDPGTKNCGYAVIEKIGRDLKLIEAGLIKIKTTVLQEQI
                     VEMTEGIDLIFKTHKIDEVSIEDMFYAFNPKTVIKLAQFRGAISLKILQNFGNFSEYT
                     PLQVKQAVTGNGKATKEQVSFMVKRLLGIKKEIKPLDITDAIAIALTHSQRIKG"
     gene            4854..>5336
                     /locus_tag="PKO10_00445"
     CDS             4854..>5336
                     /locus_tag="PKO10_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014475546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HNW65662.1"
                     /translation="MKKIGILASYNGSGFETIQKAIEDKILDAKVVVIITNNSSAGVL
                     EKAKKYEIDSFIINSKLFPNENLDLKITNILKEYGCDYIFLSGFMKKIEDNMLKTFEN
                     KIINTHPAILPSIYGGAGMYGHFVHEAVVKNGEKKSGVTIHYVNENYDEGEKILIKEL
                     F"
BASE COUNT         1820 a          750 c          640 g         2126 t
ORIGIN      
        1 ttttatatct gtaaagtggt ggttgtgcaa tatataaata acctttttca acaattggtc
       61 ttaaaaatct aaagaagaaa gttaaaagta aagtttgaat atggctacca tcaacatcgg
      121 catccgtcat aactattatt ttatggtatc ttactttttc atcattaaaa tcttcaccaa
      181 ttccacatcc tagtgctgta ataatatttc taatctcatc agattttaaa attttatcca
      241 gtctagattt ttcaacattt agaatttttc cttttaaagg taaaattgct tgataaactc
      301 tatctcttcc ttgttttgca cttcctccag cagagtcacc ttccactaga tataactctc
      361 ttatagttgg atctttactt tgacaatcag caagttttcc aggaagtgtt cctacactca
      421 tagagtcttt ttttctagtt aattctcttg cttttttagc agcttctcta ccacgtgcag
      481 ccattaaagc tttttccatt acagctcttg catgtgttgg attttcttcg aaatatttat
      541 ctaaattatc acttgtaagt ttttgtgcaa ttggtcttac ataagaactt cctaattttc
      601 cttttgtttg accttcaaat tgtggttctg gaacttttac agaaacaact gcaataagac
      661 cttctcttac atcatctcca gttatttttg catctttttc tcttgcagct gcattttcac
      721 ttaaatattt agagatactt cttgtaagtc cagctttaaa accagcttca tgagttcccc
      781 catcaattgt tctaatatta tttacaaaag atagagtttt ttcaatgtat gtatcgttat
      841 acatcatagc aatatcaact tctacaccat caactttatc actaaaatgc ataacttcac
      901 atagtgctgt ctctttattt aaatcagcaa caaattgttt aataccacct tcaaaatggt
      961 aaacttcttt aatttttgct aattcatctt ctaaagttat agatataatt ggatttaaat
     1021 aagcaacctc tttaaatctt tttttaagaa tattaaactc atattttgta acttcaaaaa
     1081 ttgaatcatc tactaaaaat tcaatagttg ttccagtttt tctaggactt tctcctataa
     1141 cttctaaagc tccttgagga attccctctt tgaactcttg atagtgaatc ttcccttctc
     1201 tataaactgt cattttaaga tgttttgata aagcatttac aactgaaacc ccaactccgt
     1261 gaagtccacc tgaaacttta tatgtatctt tatcaaattt tccaccagca tgtagaactg
     1321 ttaaagcaac agttgcagca cttattcctt cagttggatg aattgcagtt ggaattcctc
     1381 ttccatcatc ttcaattcta gcccatccat cttttgttaa agttactttt atatttctac
     1441 aaaatccagc cattgcctca tctattgagt tatcaacaac ttcataaact aagtgatgaa
     1501 gaccatttat atttgtatca ccaatataca ttcctggtct ttttctaaca gcttcaagac
     1561 cttttaaaac tttaatatta ctagcaccat aatctgacat tttattttcc ttttattttt
     1621 ctaaaactat tggcattaca attgttataa atttattatc ttccaaataa aatggaagat
     1681 ttgattcatt aaatcctatt ttaatcttat cacttgttgt catgcttaaa aaatctaaaa
     1741 tatatttagc atttactgct atataaaaat cattagggat atttaaatct atatcaactt
     1801 gtgtatttgc aacactatct gtatccaaag attcaaaaat aatagcttgt tttgtaaatg
     1861 taatttttat atttgaattt aaagatgtaa taagttttat agattcaata aatatatttt
     1921 ttggaatata tagattattt ttaagatttg atggaataat tctttcataa tctggatatt
     1981 ttccatttat aagttttgta aagaaagttg tattttcatt tgaaattact aaatttgtat
     2041 tatcatatga aatttttgca ttatctaaaa atagtttttg aatttcaaaa atagcttttt
     2101 ttggaataat taattgaact tcttcatttg acatattttc taaactattc attgctaatc
     2161 ttctagtatc tgtagcacaa aaatttattt tttgactttt tatatctaat aatgctccat
     2221 ttaattcaaa ctttggattg ttgttatcta ttgcaggaga tattttttta attgaattta
     2281 taaaattaat tgtagatata gttaaatctt ttaaattttc atatctattt atagttggat
     2341 attcatttgg atcataactt ggtaatttga atattgattt accttgtttt attattaagt
     2401 tatttgaact agtttctaaa gtaatatctt cattttttaa tcttttaata aatcctaata
     2461 gattatttcc atttactgtt gtttttccat cttcaaaatt acttattgta tcaattataa
     2521 cttcaagacc aatttcataa tcagtagctt ttatagtcat tttgctattt gcaatttcta
     2581 aatataaatg agatgtaatt gcacttgaat cttttttttc taaaaatggt tgcatttgac
     2641 ttacaacatt ttcaaaaaca gatttgttta ttactagctt catttctttc tcctattttt
     2701 tatttatttt caagtattag tagtagtcta tgtgaaaaga tgtaaatact gttttaagca
     2761 ccaaaatatc gatgttttat atagtgatta aaatcaaata tatattcaca tcttttcaca
     2821 catctaacta ttactccttg tttattaatt tattttttaa attttgaatt attaatttaa
     2881 agttttcatc tttttcaatt agctcagttg tttttttaac attatgagaa attgaactat
     2941 gatctttcat ctctaaaaac tttgcaatat caggcattga attgtgagtt aattcacgag
     3001 caagatatat aactattctt cttgcatttg ctacactaga agttcttttt ttagatttta
     3061 tatcgcttgg tttaatattt aactcatttg caactaaact tataatatct ggaagtttta
     3121 tattctcttt attctctttt atttgatctt ttaaaagatt ttgaaccatt tgtaaattta
     3181 tttcaagatt aagtaatgat gcacttgcat ttattcttat taatacaccc tcaatctctc
     3241 taattgaact atcaagagtt gttgcaataa acgaaacaat atctcgactc aaatttatcc
     3301 catttagttc tgattttttt tcaataattg caattttagt ctctagtttt ggaatttgaa
     3361 catctgttgt aagtccccat tcaaaccttg attttaatct atcaacaagt ccagctattt
     3421 gagatggaag tctatcactt gtcattacaa tttgtttttt tgcattgtgt agttcgttga
     3481 aagtgtggaa aaattcctct tgtgtactct cttttccact caaaaactgt acatcatcta
     3541 ttaaaagtaa atcacaattt ctatattttt ctctaaaatg ttccatattt tttttattta
     3601 tagaaaatat aaaatcgttc ataaactgct caattgttac atatattaca gttttatcat
     3661 tttctaaagc atgatttcca atagcttgta aaatatgtgt tttacccaaa ccagttccac
     3721 catatatgaa aagtggattg tactgaacac ctggttttga agctactgct aaagctgcat
     3781 tataagccat ttgatttgaa ggaccaacta taaatgaatc aaaagtatat gaaggattta
     3841 gtatcgtact ttctggagta tgattttgaa tttgaatatt ttgtgtctct ttttttgtct
     3901 ttttttcacc aattaatcta atttcaatat ctggtttaat attaatattt ttttcaaagc
     3961 agttttgaat aagatttgta tatttacttt ttatccatga agctatatat ttatttccta
     4021 cttcaaatat tgcaatattc tcttcaatag ataatttttt taaaacaagt tgttttaaat
     4081 atctttcgaa gtcatttttg cttgtctctt tttgaataag tgataaaaac tctttatttg
     4141 tcataataac ctaaaaaatc aaatttaatt aagcaattat tatatctaaa tattttataa
     4201 aatagcttca atggaaaaac tataaaaaat agtttaaaat gacaaaataa tttatttgta
     4261 cacaatgtga ataagtagtg aataattttt taattttata aataaaaaca tagttaaaat
     4321 acgatacaat cgaatattta aaaatgtgaa aataaaaaac aaaaaagagt gaaatagtga
     4381 aaatactagg aattgatcca ggtactaaaa attgtggata tgcagttatt gaaaaaattg
     4441 gaagagattt aaaattaata gaagcaggat taattaaaat aaaaactaca gttttacaag
     4501 agcaaattgt tgagatgaca gagggaattg atttgatatt taaaactcat aaaattgatg
     4561 aagtatctat tgaagatatg ttttatgctt ttaatccaaa aactgttata aaactagctc
     4621 aatttagagg agcaattagt cttaaaatac ttcaaaattt tggaaatttt tctgaatata
     4681 cacctcttca agtaaaacaa gcagttactg gaaatggaaa agctacaaaa gagcaggtat
     4741 cttttatggt taagcgactt ttaggaataa aaaaagagat aaaacctctt gatataacag
     4801 atgcaattgc aattgcatta acacactctc aaagaataaa aggatagaaa tttatgaaaa
     4861 aaataggaat tttggcatct tataatggaa gtggttttga gacaattcaa aaagcgattg
     4921 aagataaaat tttagatgca aaagttgttg taataattac aaataatagt agtgcaggag
     4981 ttttggaaaa ggcaaaaaag tatgaaattg atagttttat tataaattca aaactttttc
     5041 caaatgagaa tctggattta aaaataacta atattttaaa agagtatgga tgtgattata
     5101 tatttttatc aggtttcatg aaaaaaattg aagataatat gcttaaaaca tttgaaaaca
     5161 aaattataaa cacacatcca gcaattttac catcaattta tggaggagct ggaatgtatg
     5221 gacattttgt tcatgaagct gtagtaaaaa atggtgagaa aaaatcaggt gttacaattc
     5281 actatgtaaa tgagaattat gatgagggtg agaaaatttt aataaaagag ctattt
//
LOCUS       DAOQAN010000014         5263 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_19812_length_5263_cov_2.795699, whole genome shotgun sequence.
ACCESSION   DAOQAN010000014 DAOQAN010000000
VERSION     DAOQAN010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 5263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5263
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19812_length_5263_cov_2.795699"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            40..1392
                     /locus_tag="PKO10_00450"
     CDS             40..1392
                     /locus_tag="PKO10_00450"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HNW65663.1"
                     /translation="MKTWSPSSWRDFPIKQQPTYNDLVTLKQVEKELASYPPLIFAGE
                     ALNLKKQLAKVVNGEAFLLQGGDCAESFNVFNATNIKDLFKVMMQMAVVLTFSGGCPV
                     VKVGRVAGQFAKPRSSDFEDIDGLSLPSYRGDIINDIDFDIGSREPKAKKLLKAYNQS
                     AATMNLLRAFARGGMADLNQVHLWNLDFVKDNTLGAKYEEIANKISESLAFMKACGIT
                     SENTPQLNQTTLFTSHEALLLNYEEALTRRDSITKDWFNCSAHMLWIGDRTRDLDGAH
                     IEYFRGIKNPIGCKVGPSMKEDELIKLIDALNPENEAGRLNLIVRMGNEKIAEYFPKL
                     LARVESEGKKVLWSSDPMHGNTIKAENGYKTRDFEAILGEVKQFFQIHKAQGSYAGGI
                     HLEMTGQNVTECTGSKSAAITQSDLASRYHTQCDPRLNADQALELSFMIANTLKEARK
                     "
     gene            complement(1420..2346)
                     /locus_tag="PKO10_00455"
     CDS             complement(1420..2346)
                     /locus_tag="PKO10_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004318018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HNW65664.1"
                     /translation="MKIVILDRKTLGYDIDVSVFEKFGSVISFDVTKPDETKERIKDA
                     DIILTNKVYVGKDELEESKVKLICITATGTNNVDLIYAKEANIDVKNVAGYSTSSVVQ
                     LGFSMIFYFVQKLDYYKKYVDNRNWQKSDIFTHIDMPFFELDGKKVGIIGLGEIGRDF
                     AKKAKAFSCEVVYYSTSGNNQNSEYQSVSLEELLKTCDIISVHAPLNEKTKDLLGYKE
                     LNLLKDGAILLNLGRGGIINETELAKILDEKEIYCGLDVVTKEPIEQNNPLLSVKNKQ
                     RLLLTPHIAWASIEARKRLIEKVAKNIEDFLK"
     gene            complement(2399..3376)
                     /locus_tag="PKO10_00460"
     CDS             complement(2399..3376)
                     /locus_tag="PKO10_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW65665.1"
                     /translation="MDFKNSIEKFIEIFNRSNLSISKFASLIDKDRRTITSWIDRVSN
                     VEISNDIKTKICKEFRYPEYIWEDACSGDEFLKSITSIPQKEVRIIDEDYKGRLQYII
                     EHEKNRRFVIQAQFPGPMYRDSAVRKVYKTTNSSEIEELKQERINQMLRYDYDTTEWY
                     SIKSVLSFCFASIGNFFTREEKIKVLELMHELFNNNYNKKLFLFDSFSRKIYGMETTY
                     ISINVKNKILFFKSPIESVFIEIRNKSLVERMHKYYSSSIEAPSHVNFLDSVKILKIL
                     QDAVKYNNTITQAYETINRETNYGELFYNNLSIDLQKEVTPPRIAHRRD"
     gene            3550..4437
                     /gene="prpB"
                     /locus_tag="PKO10_00465"
     CDS             3550..4437
                     /gene="prpB"
                     /locus_tag="PKO10_00465"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HNW65666.1"
                     /translation="MTSAGKKFREALKASSPLQIVGTINAYQALQATKVGHKAIYLSG
                     GGIANASYGLPDLGMTMIEDVCIDIRRVTSICDTPLIVDADTGWGHAFNVARTVKEFI
                     RSGAAGMHIEDQVAAKRCGHRPNKELVSTEEMCDRIRAAVDAKKELDPDFYIIARTDA
                     HASEGQAAAIARAKAYVEAGADAIFAEAVHTLKEYKEFTDAMSVPVLANITEFGATPL
                     FTTEELASVGIAMVLYPLSAFRAMNKAALNVYQELKDKGTQEGVISTMQTRMELYDML
                     NYHAYEQKMDELFSKGKAK"
     gene            4486..>5263
                     /locus_tag="PKO10_00470"
     CDS             4486..>5263
                     /locus_tag="PKO10_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HNW65667.1"
                     /translation="MSTMSTEFKPKKSVALSGVAAGSTALCTVGNTGNDLYYRGYDIL
                     EFAEKAEFEEIAYLIVHGELPNKEQLKAYKAKLRSFRDIPEGVKIALEQLPKTCHPMD
                     VMRTGCSVLGALNQEDESHPKAEAQDIINKLMASFGSMLLYWYHFAYNGKRINVVTDD
                     DTIGGHFLHLLHGEKPRESWVKAMHVSLILYAEHEFNASTFTSRVIAGTNSDMYSCIT
                     GSIGALRGPKHGGANEVAFRIQERYSSADEAEKDIKERIAK"
BASE COUNT         1871 a          737 c          834 g         1821 t
ORIGIN      
        1 taaaattttt ccaataataa atataaaaaa ggtaaagata tgaaaacatg gagtccaagt
       61 agttggagag attttccaat aaaacagcaa ccaacttata atgatttagt aactttaaaa
      121 caagtagaaa aagagttagc atcttatcct ccacttattt ttgcaggaga ggctttaaat
      181 cttaaaaaac aacttgctaa agttgtaaat ggtgaagctt ttttacttca aggtggagat
      241 tgtgctgaat cgtttaatgt atttaatgcg acaaatataa aagatctgtt taaagtaatg
      301 atgcaaatgg cagtagtttt aactttttca ggaggttgtc ctgttgtaaa agtaggaaga
      361 gttgcaggtc agtttgcaaa accaagaagc tctgattttg aagatataga tggattaagt
      421 ctcccttcat acagaggaga tattataaat gatatagatt ttgatattgg ttcaagagag
      481 ccaaaagcaa aaaaacttct aaaagcatat aatcaaagtg cagcaactat gaatctttta
      541 agagcatttg caagaggtgg aatggctgat ttaaatcaag ttcatctatg gaatttagac
      601 tttgtaaaag ataatacttt aggtgctaaa tatgaagaga tagcaaataa aattagtgaa
      661 agtttagcat ttatgaaagc atgtggaatt acaagtgaaa atactccaca gctaaaccaa
      721 acaactctat ttacatctca tgaagcactt ttactaaatt atgaagaggc acttacaaga
      781 agagattcaa tcacaaaaga ttggtttaat tgttcggctc atatgctttg gattggagat
      841 agaactagag atttagatgg tgctcatatt gaatatttta gaggtattaa aaatccaatt
      901 ggttgtaaag ttggtccatc tatgaaagag gatgagttaa tcaaactaat tgatgcttta
      961 aatcctgaaa atgaagctgg aagattaaat ttaattgtaa gaatgggtaa cgaaaaaatt
     1021 gctgaatatt tcccaaaact tttagcacgt gttgagtctg aaggtaaaaa agttttatgg
     1081 tcaagtgatc caatgcatgg aaatactata aaagctgaaa atggttataa aactagagat
     1141 tttgaagcta ttttaggtga agtaaaacag ttcttccaaa ttcataaagc tcaaggttca
     1201 tatgctggtg gaattcacct tgaaatgact ggacaaaatg taacagagtg tacaggaagt
     1261 aaatcagcag ctattacaca aagtgattta gctagtagat atcatacaca atgcgatcca
     1321 agattaaatg ctgatcaagc tttagaacta tcatttatga tagcaaatac tttaaaagaa
     1381 gcgagaaaat aggtaaaaaa gtagatttta tctacttttt tactttaaaa aatcttctat
     1441 atttttagca actttttcga ttaatctttt tctagcttca atactagccc atgcaatatg
     1501 tggagttaaa agaagtcttt gcttattttt tacacttaaa agtggattat tttgttcaat
     1561 tggctctttt gtaacaacat caagaccaca ataaatctct ttttcatcta aaatttttgc
     1621 caactctgtt tcgtttataa ttccaccacg accaagatta agtaaaatag caccatcttt
     1681 taaaagattt agctctttgt atcctaataa atcttttgtt ttttcattta aaggtgcatg
     1741 aacagatata atatcgcaag tttttaataa ctcttctaag ctaacacttt ggtattcgct
     1801 attttgatta ttcccacttg tagagtagta aacaacttca caagaaaagg ctttagcttt
     1861 ttttgcaaaa tctcttccta tttctccaag accaataatt ccaacttttt ttccatcaag
     1921 ttcaaaaaaa ggcatatcta tatgagtaaa aatatcactt ttttgccaat tacggttatc
     1981 tacatatttt ttataatagt ctagtttttg aacaaagtaa aaaatcattg aaaaccctaa
     2041 ctgcacaaca cttgaagttg aataacctgc cacatttttt acatcaatat ttgcctcttt
     2101 tgcataaatt aaatctacat tatttgtacc tgttgcagtt atacaaatta gtttaacttt
     2161 tgactcttct agctcatctt ttcctacata aaccttattt gttaaaataa tatctgcatc
     2221 ttttattctc tcttttgttt catctggttt tgttacatca aaagagatta cactaccaaa
     2281 tttttcaaaa actgatacat ctatatcata acccaatgtt tttctatcta aaattactat
     2341 tttcattctt tatcctttag tttgtatatt cctaataaat taaaaggaat ttttcatatt
     2401 aatctcgtct atgagcaatt cgtggaggag ttacctcttt ttgcaaatca atactaagat
     2461 tattgtaaaa aagctctcca tagtttgttt cacgatttat tgtttcatag gcttgagtta
     2521 ttgtgttatt gtattttaca gcatcttgaa gtatctttaa aatctttaca gaatccaaaa
     2581 aatttacatg agaaggtgct tctattgatg aactataata tttgtgcatt ctttcaacta
     2641 agcttttatt tcttatctct ataaaaacag actcaattgg ggatttaaaa aatagtattt
     2701 tattttttac atttatagat atatatgttg tctccattcc atatattttt cttgagaatg
     2761 agtcaaataa aaatagtttt ttattgtagt tattgttaaa aagttcgtgc ataagttcta
     2821 atacttttat tttttcttct cttgtaaaaa agtttccaat acttgcaaaa caaaaactaa
     2881 ggactgattt tatagagtac cactcagttg tatcgtagtc atatcttagc atttggttaa
     2941 ttctttcttg ttttaactct tctatttcag aactatttgt tgttttgtac acttttcgta
     3001 cagcactatc tctatacatt ggtcctggaa actgtgcttg aattacaaat cttctatttt
     3061 tttcatgttc aattatatat tgaagtcttc ctttataatc ttcatcaata attctaacct
     3121 ctttttgagg tatagatgta atagatttta aaaattcatc accactacaa gcatcttccc
     3181 aaatatactc aggatatcta aactctttgc agattttagt ttttatatcg ttacttattt
     3241 ctacattact aactctatca atccacgatg ttatagttct tctatcctta tcaattaagc
     3301 tagcaaattt tgaaatactt aagtttgaac gattaaatat ctctataaat ttttctattg
     3361 aatttttaaa atccataagc ttcctttaat aaaattttcg tatattgtac aaaaataata
     3421 cataaaaaca aattatgttc aaataataaa aaatgtataa aaattgtaaa ttcttaaaac
     3481 aaaatagtac atacaaaaaa aaacaaaatt gatataattt tctctacaat atttttaaag
     3541 gatataaaga tgacaagtgc aggaaaaaaa tttagagaag ccctaaaagc ttcaagtcca
     3601 ctacaaattg ttggaacaat taatgcatac caagcattac aagcaacaaa agtaggtcac
     3661 aaagctattt acttaagtgg tggaggaatt gcaaatgctt cttatggttt accagattta
     3721 ggtatgacaa tgattgaaga tgtttgtatt gatattagaa gagttacttc tatttgtgat
     3781 actccattga tcgttgatgc tgatacaggt tggggacatg catttaatgt tgctagaact
     3841 gttaaagagt ttattagaag tggtgctgct ggaatgcaca ttgaagatca agttgctgca
     3901 aaaagatgtg gacacagacc aaataaagag ttagtatcta ctgaagagat gtgtgataga
     3961 atcagagctg ctgttgatgc taaaaaagag ttagatcctg atttctacat tattgcaaga
     4021 actgacgcac atgcaagtga aggacaagct gctgcaattg ctagagctaa agcttatgtt
     4081 gaagctggag ctgatgctat ttttgctgaa gctgttcaca ctttaaaaga gtataaagag
     4141 tttacagatg ctatgagtgt accagtttta gcaaatatta ctgagtttgg agcaactcca
     4201 ctatttacaa ctgaagagtt agcaagtgtt ggaattgcaa tggttcttta cccactttca
     4261 gcatttagag caatgaacaa agctgcatta aatgtttatc aagagttaaa agataaagga
     4321 actcaagaag gtgttattag tacaatgcaa acaagaatgg agttatacga tatgttaaac
     4381 taccacgctt atgagcaaaa aatggatgaa ttattttcta aaggtaaagc taagtaatta
     4441 gctttgaaca ttttatttaa aattaaatca aactaaggag aaaatatgag tactatgagt
     4501 acagagttta aaccaaaaaa atctgttgca ctttcaggtg tagcagctgg aagtacagca
     4561 ttatgtacag ttggaaatac aggaaatgat ttatattata gaggatatga tattttagag
     4621 tttgctgaaa aagctgaatt tgaagagatt gcatatttaa tcgttcatgg tgaattacca
     4681 aataaagagc agttaaaagc atataaagca aaacttagat cttttagaga tattccagaa
     4741 ggtgtaaaaa ttgcattaga gcaattacca aaaacttgtc atccaatgga tgttatgaga
     4801 acaggttgtt cagttcttgg agcattaaat caagaagatg agtctcatcc aaaagctgaa
     4861 gctcaagata ttattaataa attaatggca tcttttggtt ctatgctttt atactggtat
     4921 cattttgcat acaatggaaa aagaattaat gttgtaactg atgatgatac aattggtgga
     4981 catttcttac acttattaca tggtgaaaaa ccaagagaat cttgggttaa agctatgcat
     5041 gtatctttaa ttttatatgc tgagcatgaa tttaatgctt caacatttac ttcaagagta
     5101 attgctggaa caaattcaga tatgtattct tgtattactg gttctattgg tgctttaaga
     5161 ggtccaaaac acggtggagc taatgaagtt gcatttagaa ttcaagaaag atactcatca
     5221 gctgatgaag cggaaaaaga tattaaagaa agaattgcta aaa
//
LOCUS       DAOQAN010000015         5229 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_19993_length_5229_cov_3.264399, whole genome shotgun sequence.
ACCESSION   DAOQAN010000015 DAOQAN010000000
VERSION     DAOQAN010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 5229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5229
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19993_length_5229_cov_3.264399"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..1339
                     /locus_tag="PKO10_00475"
     CDS             149..1339
                     /locus_tag="PKO10_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HNW65668.1"
                     /translation="MKDIEKLDKDYVLHTYARNYVNFKKGVNATLYDDENKDYIDFTS
                     GIGVTSVGHGNARVAKRIFEQVSNLTHTSNLYAIEPQALLAKKIKELSGYDVRTFFSN
                     SGAEANEGAIKIARTYGELNFEKKRYKIITLENSFHGRTITTVKATGQSSFHQSKFAP
                     YPDGFSFNAIDDVYNAIDDETVAVMIELVQGEGGVFPFDKEKIKELAKFLKENDILLI
                     IDEVQTGVFRTGEFLASNLYDIEPDIITLAKGIGGGVPMGAVMTKHKDIWTAGDHGST
                     FGGNYLVTAAALEVLDILENLKDSGTLDETIIYFTKKLNDIFEANRDIFITHVGLGLM
                     RGLRVKDADTLASLIKTAFEFGVMVLKSGNNTLRFLPALTISKSEIDEGFIRLQKAIT
                     KIKS"
     gene            complement(1349..1807)
                     /gene="lspA"
                     /locus_tag="PKO10_00480"
     CDS             complement(1349..1807)
                     /gene="lspA"
                     /locus_tag="PKO10_00480"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014472977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HNW65669.1"
                     /translation="MKKELKIALFIFATIFIVDQVVKFGFASLGWGATGSFMNLELAY
                     NYGVAFSMLEFLAQYLKYIQLFIVVLGTIYLIKNRDVFYKYYIPIALLYAGGLSNILD
                     RFTYGAVVDYFAWHYMFEFAIFNFADVMIDLAVVIIIVMQIKESRKQKVE"
     gene            complement(1822..3156)
                     /gene="glmM"
                     /locus_tag="PKO10_00485"
     CDS             complement(1822..3156)
                     /gene="glmM"
                     /locus_tag="PKO10_00485"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012011986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HNW65670.1"
                     /translation="MKLFGTDGVRGKAGEFLDVITVIKLAQAAGIYFRKHSTTNKILV
                     GKDTRRSGYMIENALVSGLTSVGYNVIQIGPMPTPAIAYLTESMRCDAGIMISASHNP
                     FEDNGIKFFDNHGNKLSVESEKEIEKIFNDDDLLQQNQAVEKEIGSSKRIDDVIGRYI
                     VVIKSSFPKDLTLKGLRIVLDCANGAAYKVAPTILEELGADVITINNKPNGFNINDNC
                     GAMHPENVSNLVREYRADIGLALDGDADRLVVIDENGDIVDGDNLLGALSVYLKEENL
                     LEGDACVATVMSNKALEDYLEKHKIKLLRSDVGDKYVLEVMKKNGVNFGGEQSGHIIF
                     SDAAKTGDGLASALQVLALIIKSKKKASVALNPFSLYPQILENLKVSEKIPLKDIKGL
                     DEILKPIREKGIRDLIRYSGTENKIRLLLEGKNKKDVESSMKNLVDFFKKTL"
     gene            3240..3500
                     /gene="rpsT"
                     /locus_tag="PKO10_00490"
     CDS             3240..3500
                     /gene="rpsT"
                     /locus_tag="PKO10_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HNW65671.1"
                     /translation="MANHKSSEKRARQTLVKTIRNRFYKTRIKNITKDVIAAVESADK
                     EKAVVAMKVANKYFHHCVSKGILAKGTASRKVSRLQLKVNAI"
     gene            3504..4571
                     /gene="prfA"
                     /locus_tag="PKO10_00495"
     CDS             3504..4571
                     /gene="prfA"
                     /locus_tag="PKO10_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002888519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HNW65672.1"
                     /translation="MLKDKLQPFITRSEEITNLLMSPDITSDIKRMTTLSKEQSSIEP
                     IVRKAKEYIKVLEDIEENKSMLDDSELGDLAKEELKELEQKKPILEDEIKLLMIPKDP
                     NDDRNIYLELRAGTGGDEAALFVGDLFRGYLRYAENNNWKVEIMSSSDSEAGGYKEIV
                     ILVKGDHVYSKLKFEGGTHRVQRVPATESQGRVHTSAITVAVMPEVDDVEVEINESDL
                     KIDVMRASGNGGQSVNTTDSAVRITHIPSGIVVTNQDQKSQHKNKERAMKVLKAKLFE
                     IEMEKKMEAEGANRKEQVGTGDRSGRIRTYNFPQNRLSDHRINLTLYRLDYILQDGLF
                     DEVIDPLIADHQSRLIEANGL"
     gene            complement(4608..5084)
                     /locus_tag="PKO10_00500"
     CDS             complement(4608..5084)
                     /locus_tag="PKO10_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014472919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HNW65673.1"
                     /translation="MNFRKFFLTVGFSGLSPKAPGTVGSFVSLILGVILLEFLHTSSL
                     FLLSILITIIAIKQIDLYEKEIGTHDSSEIVIDELAGMWIALAICGINSENVFILAPL
                     AFIFFRIFDIWKPSIIGKIDREVKGGLGVMGDDVVAGIFAGICTAGVWQLIEKFVI"
     gene            complement(5087..>5229)
                     /locus_tag="PKO10_00505"
     CDS             complement(5087..>5229)
                     /locus_tag="PKO10_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002909858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNW65674.1"
                     /translation="SINDYIKTIVINHQNRYPDTELSKRLGISRKSLWEKRKKLEIDK
                     KK"
BASE COUNT         1930 a          788 c          772 g         1739 t
ORIGIN      
        1 taatgagttt aaaaaatcaa atcctttgga gtatggattg tttgatttaa ataggtatta
       61 ttcagcaaca agaaatgatg ataactagta agatttaacc atttatagtt ataattcata
      121 aaaaattatt aattaattag gaagagtaat gaaagatata gaaaaattag ataaagatta
      181 tgtacttcat acatatgcaa gaaattatgt aaacttcaaa aaaggtgtaa atgcaacact
      241 ttatgatgat gaaaacaaag attatataga ttttacttca ggtattggag ttacaagtgt
      301 aggacatgga aatgctagag ttgcaaaaag aatttttgaa caagtttcaa atcttacgca
      361 tacttcaaat ttgtatgcaa tagaacctca agcacttttg gctaaaaaaa taaaagagct
      421 tagtggatac gatgtaagaa cttttttttc aaatagtgga gctgaagcaa atgaaggagc
      481 tataaaaata gcaagaactt atggagagtt aaattttgag aaaaaaagat acaaaattat
      541 aactttggaa aactcttttc atggaagaac aatcacaact gtaaaagcta caggtcaaag
      601 ctcttttcat caaagtaagt ttgctccata cccagatgga ttttcattta atgcgataga
      661 tgatgtttat aatgcaatcg atgatgaaac agttgctgtt atgatagagc ttgtgcaagg
      721 tgagggtgga gtttttccat ttgataaaga aaaaattaaa gagttggcaa aattcctaaa
      781 agaaaatgat attttattaa taattgatga agttcaaaca ggagtattta gaactggaga
      841 gtttttagct tcaaatttat atgatataga gccagatatt ataactttgg caaaaggtat
      901 aggaggcggt gttcctatgg gagctgttat gactaaacat aaagatattt ggacagctgg
      961 tgaccatgga agtacttttg gtggtaacta tttggtaacc gcagctgctt tagaagtttt
     1021 ggatatttta gaaaatttaa aagatagcgg aactttggat gaaactatta tatattttac
     1081 aaaaaaatta aatgatattt ttgaagcaaa tagagatatt tttataactc atgttggatt
     1141 aggtcttatg agaggattaa gagtaaaaga tgctgatact ttagcaagtt taataaaaac
     1201 tgcttttgaa tttggagtta tggttcttaa atcaggaaat aatactttaa gatttttacc
     1261 agcactaaca atctctaaaa gtgaaataga tgaaggattt ataagacttc aaaaagctat
     1321 tactaagata aaatcataaa aaagtggatt actccacttt ttgttttctg ctctctttta
     1381 tttgcataac tataattata acaaccgcta aatctatcat aacatcagca aaattaaata
     1441 ttgcaaactc aaacatatag tgccatgcaa aataatctac aaccgcacca taagtaaatc
     1501 tatctaaaat atttgataaa ccaccagcgt ataaaagtgc aataggaata tagtatttat
     1561 aaaaaacatc tctattttta ataagataaa tagttcctaa aactactata aaaagttgta
     1621 tatattttaa atattgcgcc aaaaattcta gcatagaaaa tgctacgcca taattatatg
     1681 caagctctag attcataaag cttccagtag ctccccatcc taaagaggca aaaccaaatt
     1741 ttacaacttg atctacaata aaaatagttg caaaaataaa caatgcaatt tttaactctt
     1801 ttttcaaatt atttcctatc tttatagtgt ttttttgaaa aaatctacta aatttttcat
     1861 agatgattca acatcttttt tatttttacc ttcaagtaaa agtctaattt tattttcagt
     1921 tcctgaatat cttatcaaat ctcttatacc tttttctcta attggtttta aaatctcatc
     1981 taaacctttt atatctttta atggtatttt ttcactaact tttaaatttt ctaatatttg
     2041 aggatataaa ctaaatggat ttaaagcaac acttgctttt ttctttgatt ttattatcaa
     2101 agctaagact tgaagtgctg aagctaaacc atctcctgtt tttgcagcat ctgaaaatat
     2161 gatatgtcca ctttgctcac caccaaaatt aacaccattt tttttcataa cttcaagtac
     2221 atatttatca ccaacatcac ttcttaaaag tttgatttta tgtttttcta aataatcttc
     2281 taatgcttta tttgacataa ctgtagcaac acaagcatct ccttctaaaa ggttttcctc
     2341 ttttagatat acacttaaag ctcctaaaag attgtcacca tcaacgatat ctccattttc
     2401 atcaataaca accaatctat cagcatcgcc atcaagtgct agaccaatat ctgctctata
     2461 ttcacgaact aaatttgaaa cattttcagg atgcatagct ccacaattat cgttaatatt
     2521 aaaaccattt ggtttattat ttattgtaat tacgtcagct cctaactctt ccaaaattgt
     2581 aggagcaact ttataagcag caccatttgc acaatcaagg acaattctta atccttttag
     2641 agttaaatct tttgggaaag aagattttat aactacaatg tatcttccaa taacatcatc
     2701 tattcttttt gaagaaccaa tctctttttc aacagcttga ttttgttgta gtaaatcatc
     2761 atcattaaaa attttctcaa tctctttttc actttcaaca ctaagcttgt taccatgatt
     2821 atcgaaaaat tttattccat tatcttcaaa aggattatga gaagcgctaa tcataattcc
     2881 agcatcacat ctcatgcttt ctgtaagata tgcaattgca ggtgttggca taggtcctat
     2941 ttgaattaca ttgtaaccaa cagaagttaa tccactaaca agtgcatttt caatcatata
     3001 cccacttctt cttgtatctt tacctacaag aattttattt gttgttgagt gcttcctaaa
     3061 ataaattcca gctgcttgcg ctaattttat aactgtaata acatctaaaa actctccagc
     3121 ctttcctcta actccgtctg ttccaaatag tttcattaaa tcttcctaaa attaaactct
     3181 atttaagtta ttttggatag tattctatcc tttaaaaatt tataaagagg ttaaataaaa
     3241 tggcaaatca taaatcttct gagaagagag ctagacaaac attagtaaaa actataagaa
     3301 atagatttta caaaacaaga attaaaaaca tcactaaaga tgttattgca gcagtagaaa
     3361 gtgctgataa agaaaaagca gtagttgcta tgaaagttgc aaataaatac ttccaccact
     3421 gcgtttcaaa aggtatttta gcaaaaggta cagcttctag aaaagtaagc agacttcaac
     3481 taaaagttaa tgctatataa tttatgctaa aagacaaact ccaacctttt attactagat
     3541 cagaggagat tacaaattta ttgatgtctc ctgacataac tagtgatata aaaagaatga
     3601 caactctgtc aaaagagcaa tcaagtatag aaccaattgt tagaaaagca aaagagtaca
     3661 taaaagttct tgaagatatt gaagagaaca aaagtatgct tgatgattct gagctgggtg
     3721 atttagcaaa agaagagtta aaagagcttg agcagaaaaa acctattttg gaagatgaga
     3781 taaaacttct tatgattcca aaagacccaa atgatgatag aaatatatat ttggagctta
     3841 gggctggaac aggtggagat gaagctgcac tttttgttgg agatttgttt cgtggttatt
     3901 tgcgatatgc agaaaacaat aactggaaag ttgaaattat gagttcaagt gatagtgaag
     3961 ctggtggtta taaagagatt gtaatactcg taaaaggtga ccacgtttat tctaagctaa
     4021 aatttgaagg tggaactcac agagttcaaa gagttcctgc aacagagtct caaggaagag
     4081 ttcacacatc tgctattaca gttgctgtta tgccagaggt tgatgatgtt gaagttgaga
     4141 ttaacgaaag cgatttaaaa atcgatgtaa tgcgtgcaag tggaaatggt ggacaatctg
     4201 tgaatactac tgattctgct gtaagaatta ctcatattcc atctggtatt gttgtaacaa
     4261 atcaagatca aaaatctcag cataaaaata aagagagagc tatgaaagtt ctaaaagcta
     4321 aactttttga aattgagatg gaaaaaaaga tggaagctga gggtgcaaat agaaaagagc
     4381 aagtaggaac tggagataga agtggaagaa ttagaactta taatttccca caaaatcgtc
     4441 taagtgacca cagaataaat ctaactttat atcgacttga ctatattctt caagatggtc
     4501 tgtttgatga ggtaattgat cctcttattg ctgatcatca atcaagactt atcgaagcaa
     4561 atgggctata aaagcccttt ttgttttaat ctacaaatta tatttttcta aataacaaat
     4621 ttttcaatta gttgccaaac tccagctgta caaattccag caaaaattcc agcaacaaca
     4681 tcatcgccca ttactcctaa tccacctttt acttccctat caatttttcc aataatagat
     4741 ggtttccaaa tatcaaagat tctaaaaaat ataaaagcaa gaggagctaa aatgaaaaca
     4801 ttttcactat ttattccaca aatagctaaa gctatccaca taccagctaa ttcatctatt
     4861 acaatttcac tactatcgtg agtaccaatc tctttttcat aaagatctat ttgttttata
     4921 gctattatag ttattaaaat agatagtaga aacagagatg atgtgtgtaa aaactcaagt
     4981 aaaataactc ctaaaattaa agatacaaaa cttccaacag ttccaggtgc ttttggactt
     5041 aatccactaa atccaacagt taaaaaaaat tttctaaaat tcatagttac ttctttttat
     5101 caatttcgag tttttttctc ttttcccaaa gagattttct agatattcct aacctttttg
     5161 aaagttctgt atcagggtat ctattttgat gatttatcac tattgttttt atataatcgt
     5221 ttattgaca
//
LOCUS       DAOQAN010000016         5126 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_20551_length_5126_cov_2.978505, whole genome shotgun sequence.
ACCESSION   DAOQAN010000016 DAOQAN010000000
VERSION     DAOQAN010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 5126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5126
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20551_length_5126_cov_2.978505"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="PKO10_00510"
     CDS             complement(<1..335)
                     /locus_tag="PKO10_00510"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014474782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HNW65675.1"
                     /translation="MKDLVAKRYVKALVDGRDLNSVALISEKLNEVSKAFKSDKFNSI
                     LSSSDINEVEKTKFILSMIDKADKSLENFIKLLGEKRRLNIIPEIAFELKTQIAKMNS
                     SYVGTVYTN"
     gene            complement(335..853)
                     /locus_tag="PKO10_00515"
     CDS             complement(335..853)
                     /locus_tag="PKO10_00515"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF006292.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HNW65676.1"
                     /translation="MKRVLLLLAMAMVPVALFASEGAETNYDIVQRTVNFIIFAAILW
                     YLLADKIKAFFANRTLGIQSELDKVQDSLKASKDRVAEAQKKLDDAKKISAEIIESAK
                     SDIENIKQKVSASVDADIANLSKNLDEMIKVEISKAKKQVVAEILDELLNSENIKLSQ
                     DELVNIVLKRVA"
     gene            complement(863..1285)
                     /locus_tag="PKO10_00520"
     CDS             complement(863..1285)
                     /locus_tag="PKO10_00520"
                     /inference="COORDINATES: protein motif:HMM:NF006293.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B'"
                     /protein_id="HNW65677.1"
                     /translation="MLDISPVLLLSSGIIFLLVVARLNSCLFKPILQHMDERSAQIKK
                     DLEDSKSNSADVDGFLAEANDLLSKAKREAAAIREQAYKEAKDSADVKLASAKLNLEA
                     KSAEFAKSLQDETKALKASLLSSMPQFNESLKSKLSSI"
     gene            complement(1392..2246)
                     /locus_tag="PKO10_00525"
     CDS             complement(1392..2246)
                     /locus_tag="PKO10_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNW65678.1"
                     /translation="MALGRGLGELLGEVETAYGKSSGNSNSGVNKIEVSLIKPNPNQP
                     RKIFDEEKLQELSASIKEHGLLQPIVVVEDEDGTYTLIAGERRLRAHKLANIEEIKAI
                     IVDKDEFKLRELALIENIQRDDLNIIELAFCYAQLLNEHNITHEELSKKVFKSRTSIT
                     NTLRLLQLSSYVQQFLATDKISAGHAKMMIGLTTEDQKKICDTIIGQKLSVRETEKLI
                     KDLKEKDTPKPKKEKVTNSYNISNLKSFAEFLKNDKIKAKIDKNSIKIEFNSQEDIDK
                     ISSYFKIQ"
     gene            complement(2269..3045)
                     /locus_tag="PKO10_00530"
     CDS             complement(2269..3045)
                     /locus_tag="PKO10_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNW65679.1"
                     /translation="MTEIISIANQKGGVGKTTTAVNLSAALALDGKKVLLIDADPQAN
                     ATTSLGFHRDTYEYNIYHVMLGTKELSEIILDSEIENLKVAPSNIGLVGIEKEFYKNT
                     KERELVLKRKIDTVKADYDYIIIDSPPALGPITINTLGASTSVLIPIQCEFFALEGLA
                     QLLNTIKLVKQTINKNLQIKGFLPTMYSAQNNLSKQVFADLAQHFENKLFKVDDDSYV
                     VIPRNIKLAESPSFGKPIMLYDTTSIGTKAYTNLAKAIIG"
     gene            complement(3042..3677)
                     /locus_tag="PKO10_00535"
     CDS             complement(3042..3677)
                     /locus_tag="PKO10_00535"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HNW65680.1"
                     /translation="MKIIRLNEVNSTQSYLKEYIQKNGYNNPLCVTSKIQTAGVGSRG
                     NSWIGKEGNLFFSFVVSKDNLPKDLPLQSASIYFTYILKDILQKSGSKLFLKWPNDFY
                     IKNRKIGGAITSTNKELLFCGIGLNLIEVDDSFGKLDISIDIDEILNLYFIEIEKKIP
                     WKQIFSLFKIEFMHSKNFQATVDGVKVSLENAILNSDGSIQIENKKVFSLR"
     gene            3752..4645
                     /locus_tag="PKO10_00540"
     CDS             3752..4645
                     /locus_tag="PKO10_00540"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HNW65681.1"
                     /translation="MLDPVLPIAIYLLFGYLFKIIFQDNSKQLVDFIIYFSLPAIVFS
                     KIYPLQLDTKILWLILMFMAIIFFNLFLSYCVGKIMRLNRVTLATFMIMGTFGNTSFI
                     GFSYIDAFYGQDYIVYGVIYDIFGSFLLLVSVGMIIITWGSGRKNSILNISKSIFLFP
                     PMIIFFITIFAKNFEVPKFIIYTSQNLGSTLVPIAMIAIGMKLELKHIFSRLHIVTVA
                     VVLKMLIVPIIILFTFKYFYGVDETWVKVTLIEVAMPPMTMAAVLAIKGGLDEKIAIN
                     SLVLGVIVSLFTITLFTSYLA"
     gene            complement(4663..5064)
                     /locus_tag="PKO10_00545"
     CDS             complement(4663..5064)
                     /locus_tag="PKO10_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014474920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNW65682.1"
                     /translation="MSKEIVLDDYAFLVSETDEKGIIIFANDDFCKIAGYGIDELIGK
                     PHNTVRHPDMPKAAFKDLWETVKKGNIWTGYVKNATKDGDYYWVFATVYPTITSEGTK
                     GYLSCRRKASIEEIKTHENLYKQLKMNEKAA"
BASE COUNT         1624 a          814 c          592 g         2096 t
ORIGIN      
        1 ttatttgtat aaacagttcc aacataacta ctattcatct ttgcaatttg agtttttaac
       61 tcaaaagcta tttcaggaat aatatttaat cttctttttt caccaagtaa tttaataaag
      121 ttttccaaac ttttatctgc tttatcaatc atagataaaa taaatttagt tttttcaact
      181 tcatttatat cagaagatga caatatagag ttaaatttat cacttttgaa agctttcgaa
      241 acttcgttta atttctcaga aatcaaagca acgctattta aatctcttcc atcaaccaaa
      301 gctttcacat atcgttttgc tactaaatct ttcattatgc taccctttta agaacaatat
      361 ttactaactc atcttgagat agtttaatat tttctgaatt caacagctca tcaagtattt
      421 cagcaacaac ttgtttctta gctttagaaa tttcaacttt tatcatctca tctaaatttt
      481 tacttaaatt tgcgatatct gcatctacag atgctgaaac tttttgttta atattttcaa
      541 tatcagactt tgcactttcg attatttcag cagaaatctt ttttgcatca tctaattttt
      601 tttgagcttc agcaactcta tctttagaag cttttaaact atcttgaact ttatctagtt
      661 cagattgaat tcccaaagtt ctattagcaa aaaatgcttt aattttgtca gcaagtaaat
      721 accataaaat cgcagcaaat ataataaaat taacggttct ttgaactata tcgtagttcg
      781 tttccgcacc ttcgcttgca aataatgcaa ctggaaccat agccatagct aatagtaata
      841 gtactctttt cattatcact tcctatattg aactaagctt agattttaag ctttcgttaa
      901 attgtggcat tgatgacaat aaagaggctt ttaatgcttt cgtctcatct tgtaaacttt
      961 tagcaaattc agcagatttt gcttctaaat ttaatttagc acttgcaagt ttaacatcag
     1021 cactatcttt tgcttcctta taagcttgct ctctaattgc agctgcttct cttttagctt
     1081 ttgaaagcaa atcatttgct tctgctaaaa aaccatctac atcagcacta tttgattttg
     1141 aatcttccaa atcttttttg atttgagcag atctttcatc catatgttga agtataggtt
     1201 tgaaaagaca actgtttagc ctagcaacca ctaaaagaaa gatgatacca gaactaagca
     1261 atagtacagg acttatgtct aacattcatt cctccatatt tttacagttt agtttaacta
     1321 aaacgttttt attttatcaa atttatctat aaatttatat taaaatagtt aagaatatgg
     1381 atttttttat tttattgtat cttaaagtaa ctacttatct tatctatatc ttcttgagag
     1441 ttgaattcaa tttttattga atttttatct attttagctt tgattttatc attttttaaa
     1501 aactcagcaa atgattttaa attactaatg ttgtaactat ttgtaacttt ttcttttttt
     1561 ggttttggag tatctttctc ttttaaatct tttattaatt tttctgtctc tcttacagaa
     1621 agtttttgcc caataatagt atcgcaaatc tttttttgat cttcagttgt taatcctatc
     1681 atcatttttg catgacctgc actaatttta tctgtagcca aaaattgttg aacatatgag
     1741 cttaattgta gtaatcttaa agtatttgta atagaagttc gacttttaaa aacttttttt
     1801 gatagctctt catgagtgat attgtgttca tttagtagtt gtgcataaca aaaagctaac
     1861 tctataatat ttaaatcatc tctttgaata ttttctatta aagccaactc tcttagctta
     1921 aactcatctt tatctactat aattgctttt atctcttcaa tatttgcaag cttatgagct
     1981 cttaatcttc tctctccagc aattaaagta taagttccat cttcatcttc aacaacaaca
     2041 attggttgca aaagaccatg ctcttttata gaagcgctta gctcttgaag cttctcttca
     2101 tcaaagattt ttcttggctg atttggattt ggtttaatca aagatacttc aattttatta
     2161 actccactat ttgagtttcc actagattta ccataagctg tctcaacttc acctaaaagt
     2221 tctcctaatc ctcttcctaa tgccatctta ttttcctctt aaaaaaattt aaccaataat
     2281 tgcttttgct aagtttgtat atgcttttgt accaatagat gtagtatcat aaagcataat
     2341 tggtttacca aaactaggac tctccgctaa tttaatattt cttggaatta caacatacga
     2401 gtcatcatct actttaaata gcttgttttc aaaatgttgt gctaaatctg caaaaacttg
     2461 ttttgataag ttgttttgag cagaatacat agttggtaaa aaacctttta tttgaagatt
     2521 tttatttatc gtttgcttta caagttttat tgtatttaaa agctgtgcta aaccttctaa
     2581 agcaaaaaat tcacactgaa ttggtattaa aacagaagtt gaagcaccca aagtatttat
     2641 tgtaattggt cctaaagctg gaggtgaatc aattattatg taatcataat cggctttaac
     2701 tgtatcgatt ttccttttta atacaagttc acgctcttta gtgtttttgt aaaactcttt
     2761 ttctattcct acaagaccta tatttgaagg tgcaactttt aaattttcta tttcagagtc
     2821 caaaataatc tctgaaagct cttttgttcc aagcattaca tgataaatat tatattcata
     2881 tgtatctcta tgaaaaccta aagaagttgt agcatttgct tgtggatcag catctattaa
     2941 aagaactttt ttcccatcta gagcaagggc tgcactaaga tttacagcag ttgtagtttt
     3001 tcctactccg cctttttgat ttgctattga aataatttct gtcatcttaa actaaatacc
     3061 tttttgtttt caatttgtat agaaccatcg ctatttaaaa tagcattttc aagtgaaact
     3121 ttaacaccat caacagttgc ttgaaaattt ttagaatgca taaattctat cttaaaaaga
     3181 ctaaaaattt gcttccacgg aatttttttt tctatctcta taaaatataa atttaaaatt
     3241 tcatcaatat ctatgcttat atcaagtttt ccaaaactat catctacttc aataagattt
     3301 aaacctatac cacaaaatag taactcttta tttgtagaag ttattgctcc accaattttt
     3361 ctatttttta tataaaaatc atttggccat tttaaaaata gttttgaacc actcttttgt
     3421 aaaatatcct ttaaaatata tgtaaaatat atactagcac tttgaagtgg taaatctttg
     3481 ggtaaattat ctttacttac aacaaaagag aaaaagagat ttccctcttt tccaatccaa
     3541 gagtttcccc tgcttccaac tcctgcagtt tgtatttttg aagttacaca taatggattg
     3601 ttataaccat ttttttgtat atactctttt aaatagcttt gtgttgaatt aacttcattt
     3661 aatcttataa ttttcatttg aagattatat ataattaaag tagaagatta tattaattat
     3721 aatgaaatcc ttattttcat aaaggcagaa gatgttagac ccagttttac caatagctat
     3781 atacctactt tttggttatt tatttaaaat tatttttcaa gataattcaa aacaacttgt
     3841 agattttatt atatatttct cccttccagc aatagttttt tcaaaaattt atccactaca
     3901 gctagataca aaaattttat ggcttattct tatgtttatg gcaataatat tttttaatct
     3961 atttttatca tactgtgttg gaaaaattat gagactaaac agagttactc tagccacttt
     4021 tatgattatg ggaacttttg gaaatacctc ttttataggt ttttcttata tagatgcttt
     4081 ttatggtcaa gattatattg tttatggagt tatttatgat atttttggat ctttcttact
     4141 tttagtatca gttggaatga ttataataac ttggggaagt ggaagaaaaa actctatttt
     4201 gaatatttca aaatcaatat ttctattccc accaatgatt atctttttta ttacaatatt
     4261 tgcaaaaaac tttgaagttc caaaatttat aatctacact tcacaaaatc ttggctcaac
     4321 tttagttcct attgcaatga ttgctattgg gatgaaactt gaattaaaac atatattttc
     4381 aaggttacat attgtaacag ttgctgttgt tttaaaaatg ctcattgtac caattataat
     4441 tttatttaca tttaaatatt tttatggagt agatgaaaca tgggtaaaag taacattaat
     4501 agaagttgca atgccaccaa tgactatggc tgctgtttta gctataaaag gtggattaga
     4561 tgaaaaaatt gctataaatt ctctagtttt aggtgttatt gtaagtctat ttacgataac
     4621 tttgtttact tcatatttag cttaagattt attatttaaa tcttaagctg ctttttcgtt
     4681 catttttaat tgtttatata aattctcatg agtttttatc tcttcaatgc tagcttttct
     4741 tctacaagat aaatatccct ttgttccttc acttgtaatt gttggataaa ctgttgcaaa
     4801 tacccaatag taatctccat ctttagttgc atttttaacg tatcctgtcc agatattacc
     4861 tttttttaca gtttcccaca aatctttaaa tgcagctttt ggcatatctg gatgtcttac
     4921 agtattgtga ggtttaccta ttaactcatc aattccatat cctgcgattt tacaaaaatc
     4981 atcatttgca aaaataatta ttcctttttc atctgtttca cttactaaaa aagcataatc
     5041 atccaaaact atctcttttg acataatatt tttcctttaa ttgttttaat aaaatttaat
     5101 tttttaattg atttactcta aattta
//
LOCUS       DAOQAN010000017         5075 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_20845_length_5075_cov_3.005378, whole genome shotgun sequence.
ACCESSION   DAOQAN010000017 DAOQAN010000000
VERSION     DAOQAN010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 5075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5075
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20845_length_5075_cov_3.005378"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..748)
                     /locus_tag="PKO10_00550"
     CDS             complement(<1..748)
                     /locus_tag="PKO10_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNW65683.1"
                     /translation="MQYDIIVVGAGHAGIEAALAGARLGKKTLLITMLVEQIGAASCN
                     PAIGGLAKGHLVRELDAIGGEMGLCTDATGIQFRILNDSKGAAVQGTRAQIDMDEYKE
                     YMRKVCHNTANLDIYQDEVSYLIVENDEVLGVKTKLGEEFKSKKTILTTGTFMRGLIH
                     IGENTYEAGRAWELPSTTLSIQLKELGLNVGRLKTGTPSRLDANSIDFSVMETHGGDI
                     KPTPFSFRTPRKTFNPTQYPCYVTYTNLDTH"
     gene            854..1462
                     /gene="ribE"
                     /locus_tag="PKO10_00555"
     CDS             854..1462
                     /gene="ribE"
                     /locus_tag="PKO10_00555"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014469474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HNW65684.1"
                     /translation="MFTGLIRELAKVESFSNNILTLKAEYIPKIGDSIAVNGACLTVI
                     KISNNSFSVELSPESTSLLALENYKNEVHIEPAMQMGDRFEGHIVQGHVDCIGVVEKI
                     EKNGNSTDFYISLPKEFIKFVIPKGSITIDGVSLTVNDVFETSFRLTIIPHTIQNTIF
                     RNYKSGSKVNIETDMFARYIYNMFKKDNKLSWGDVDRIMARY"
     gene            complement(1482..2321)
                     /gene="mreC"
                     /locus_tag="PKO10_00560"
     CDS             complement(1482..2321)
                     /gene="mreC"
                     /locus_tag="PKO10_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012148062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HNW65685.1"
                     /translation="MKRFLFLLLIVFAGLSYLFELDEFLAKHFKVFGNLKESYINTYI
                     SVSQKFANHFEHERLIRELQNENLELKEYKALYNTTVSAIEDLRNLIQNIEIPEINSK
                     IEVIRVLSYVNFDDFTSVWLDKAPTDNKILGLIADNYAAGISMTKNGKRIGLLNGNKD
                     CTYAVFIGNERNPGIATSNDESSDLLSVKFIPVWADINVGDEVITSGMDNIFYEGLRV
                     GKVVEIKDFPEMKIATIQPYAKPLKKRFFYTYEANNNIDNQEKSILENLDESKKVENK
                     EQK"
     gene            complement(2334..3374)
                     /locus_tag="PKO10_00565"
     CDS             complement(2334..3374)
                     /locus_tag="PKO10_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014469476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNW65686.1"
                     /translation="MILDKFIGVFSNDMAIDLGTANTIVSVKGKGIIINEPSVVAVQR
                     DRQGKDKILAVGQEAKRMLGKTPINIQAVRPMQDGVIADFEMTERMIRYFIEKAHSRK
                     SFIRPRVIICIPYGITQVERKAVEESALSAGAREVFLVEEPMAAAIGAGIPVSDPEGH
                     VVVDIGGGTTEIGVTSLGGLVLCKSVKVGGDKIDRSIIEYVRQNYNLFIGERTAETVK
                     IEVGAAIKLDPELKIQIKGKDHSGYLSTIELGSEGIRIAIKEPLKEIASAIRSVLENM
                     PPDLAGDVVDNGIIITGGGALIRGLDKYLTEIVKIPVRIADEPLLSVAYGTSQVLDEP
                     GLLKIIEDMKNS"
     gene            complement(3380..4600)
                     /gene="clpX"
                     /locus_tag="PKO10_00570"
     CDS             complement(3380..4600)
                     /gene="clpX"
                     /locus_tag="PKO10_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012148063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HNW65687.1"
                     /translation="MSKLICDFCGASDTKANPIITGDNACICKACVGAAYDIMSGDFV
                     DGETKSKPEKVENIKIKTPAELKKILDEYVIGQERAKKVLSVAVYNHYKRIFRKDELQ
                     GDIELNKSNVLLIGPTGSGKTLLAQTISKYLDVPLAIADATSLTEAGYVGDDVENVVT
                     RLVQAAGGDIEKAQRGIIFIDEIDKIARMSENRSITRDVSGEGVQQALLKIIEGAVVN
                     VPPKGGRKHPGQDSLQVDTTNILFICGGAFDGLEDIIKRKQGSNVLGFNQDKKSKVEQ
                     DKIISNVETDDLVKYGLIPELIGRLHMIATLNEITKEDMVHILTEPKNALIKQYIKLF
                     EMDNVTLEFEKDALLELANLAIIRKTGARGLRSILEDIMLDIMFDLPKYKSKKIVITK
                     DVVLKTEEPKVIKG"
     gene            complement(4593..>5075)
                     /locus_tag="PKO10_00575"
     CDS             complement(4593..>5075)
                     /locus_tag="PKO10_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008242906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein]--UDP-N-
                     acetylglucosamine O-acyltransferase"
                     /protein_id="HNW65688.1"
                     /translation="IGNCNLLMNYVHVAHDCVIGNNSIFANGVTLAGHVECGDFVVIG
                     GLTPIHQFCKIGTGVMVGGASAVAQDIPPFCLAEGNKALVKGLNLVGLRRRLENREDV
                     DALKHAYRELFNMRRPLQEAALDLYENDKNPYVKEMANFVLNTKRGIPFERKIKSENE
                     "
BASE COUNT         1614 a          939 c          600 g         1922 t
ORIGIN      
        1 cgtgagtatc aagatttgta tatgttacat aacaaggata ttgtgttggg ttaaaagttt
       61 ttctaggtgt tctaaaagaa aaaggtgttg gttttatatc tccaccatga gtctccataa
      121 ctgaaaaatc aatactattt gcatcaagtc ttgaaggtgt tccagtttta agtcttccta
      181 catttaatcc aagctcttta agttgaattg aaagagtagt agatggcaac tcccaagcac
      241 gtcctgcttc ataagtattc tctccaatat gaattaaacc tctcataaaa gttccagttg
      301 taagaatagt ttttttagat ttaaactctt ctccaagttt tgtttttact cctaagactt
      361 catcattttc aacgattaaa taactaacct catcttgata aatatctaaa ttagcagtat
      421 tgtgacaaac ttttcgcata tactccttgt attcatccat atcaatttga gcacgtgtac
      481 cttgaacagc tgcacctttt gaatcattta atattctaaa ctgaattcca gtagcatctg
      541 tacaaagtcc catctctcca ccaattgcat ctagttctct tactaaatgt ccttttgcta
      601 aacctccaat agcagggtta caactagcag ctcctatttg ttcaacaagc attgtgatta
      661 ataaagtttt ttttccaagt cttgcacctg ctagtgctgc ttcaatacca gcatgtcctg
      721 caccaacaac gataatatcg tattgcatta aattttcctt tttaaagagt atcagatttt
      781 taaatattag taaaaattag cttcttatga atttttggct attataacca aattttatct
      841 aaaggttttt taagtgttta cagggcttat aagagaatta gcaaaagtag aaagcttttc
      901 aaacaatatt ttaactctaa aagcagaata tatacctaaa attggagatt ctattgctgt
      961 aaatggagct tgtttaactg ttattaaaat ttcaaataat agttttagtg tagagttatc
     1021 tcctgaaagc acatctcttt tagctttaga aaactacaaa aacgaagttc atatagaacc
     1081 tgctatgcaa atgggcgata gatttgaagg tcatattgtc caaggacatg ttgattgtat
     1141 tggtgtagtt gaaaaaatag agaaaaatgg aaactctact gatttttata tatctttacc
     1201 aaaagagttt attaaatttg ttattccaaa aggaagtatt actattgatg gagtttcact
     1261 tacagttaat gatgtttttg aaacttcttt tagattaaca ataattccac atactataca
     1321 aaatacaatt tttagaaatt ataaatcagg aagtaaagtt aatatagaaa ctgatatgtt
     1381 cgctagatat atttataata tgtttaaaaa ggataataaa ttatcttggg gagatgttga
     1441 tagaataatg gctagatact aattttatat attaaaaatt actatttttg ctctttattc
     1501 tctacttttt ttgactcatc taaattttct aaaatactct tctcttgatt gtcaatgttg
     1561 ttatttgctt cataagtgta aaagaatctc ttttttagag gttttgcata tggttgtatt
     1621 gttgcaattt tcatctctgg aaaatctttt atctctacaa ctttaccaac tctaagtccc
     1681 tcataaaaaa tattatccat tcctgaagtt ataacttcat ctccaacatt tatatcagcc
     1741 caaacgggaa taaattttac agataataaa tctgatgatt catcatttga agttgcaatt
     1801 ccaggatttc tctcatttcc tataaaaaca gcataagtac aatctttatt tccatttaga
     1861 agtcctattc ttttaccatt tttagtcata gagattccag cagcataatt gtcagctatt
     1921 aatcctaata ttttattatc agtaggagct ttatctaacc aaacacttgt gaaatcatca
     1981 aaatttacat atgataaaac tctaataact tcaattttag agttaatttc tggaatctca
     2041 atattttgta ttaaatttct taaatcttct attgctgaca ctgtagtatt atatagtgct
     2101 ttatactctt ttagttctaa attctcattt tgaagttctc ttattagtct ttcatgctca
     2161 aagtggtttg caaacttttg ggatacactt atgtatgtat ttatatatga ctcttttaaa
     2221 tttccaaata ctttaaagtg ttttgctaga aactcatcta attcaaatag ataagatagt
     2281 ccagcaaata caattagtag taaaaataaa aatctcttca ttttttaaat aatttaagag
     2341 ttcttcatat cttcaatgat ttttaaaagt cctggctcat ctaaaacttg acttgttcca
     2401 taagcaacag ataaaagtgg ctcatctgca attcttacag ggatttttac aatttcagtt
     2461 agatatttat ctaatcctct aattaaagct cctccacctg ttattataat tccattatca
     2521 acaacatctc ctgctaaatc tggtggcata ttttctaaaa cacttctaat tgcagaagct
     2581 atctctttta gaggctcttt tattgcaatt ctaattcctt cacttccaag ctcaattgta
     2641 gataagtatc cagagtgatc tttaccttta atttgtattt ttaattcagg atcaagttta
     2701 attgcagctc caacctctat ttttacagtt tcagctgttc tctctcctat aaataaatta
     2761 tagttttgtc ttacatattc aataattgat ctatcaattt tatctccacc aactttaact
     2821 gatttacata aaactaaacc acccaaagat gtaacaccaa tctctgttgt acctccacca
     2881 atatcaacaa caacatgacc ttctggatca cttactggaa taccagctcc aattgctgct
     2941 gccattggct cttccacaag gaaaacttct ctagcacctg cacttaaagc tgactcttca
     3001 actgcttttc tctcaacttg agttatacca taaggaatac aaataattac tctaggtctt
     3061 ataaatgatt ttcttgagtg tgctttttca ataaaatatc taatcattct ttcagtcatc
     3121 tcaaaatcag caataactcc atcttgcata ggacgaactg cttgaatatt tattggagtt
     3181 tttcctaaca ttctttttgc ttcttgacca actgccaaaa ttttatcttt accttgtcta
     3241 tctctttgta ctgctacaac agaaggttca tttataataa tacctttacc ttttacagat
     3301 acgattgtat ttgctgtacc caaatctatt gccatatcgt ttgaaaaaac gcctataaat
     3361 ttatctaaaa tcatattatt tatcctttta taacttttgg ttcttctgtt tttaaaacta
     3421 catcttttgt aatcactatt tttttactct tatattttgg aagatcaaac ataatatcaa
     3481 gcataatatc ttccaaaatc gacctcaaac ctcttgcccc tgtttttcta ataattgcta
     3541 aatttgctaa ctctaaaagg gcatcttttt caaattctaa agttacatta tccatctcaa
     3601 ataattttat atattgtttg attaaggcat tttttggctc tgttaaaata tgaaccatat
     3661 cttcttttgt gatctcattt aaagttgcaa tcatatgaag ccttccaata agttcaggaa
     3721 taagcccata ttttactaaa tcatctgttt caacatttga gataatctta tcttgctcaa
     3781 cttttgattt tttatcttga ttaaatccta aaacatttga accttgtttt ctttttatta
     3841 tatcttcaag cccatcaaaa gctccaccac aaataaacaa aatatttgta gtatctactt
     3901 gaagtgaatc ttgtccagga tgttttcttc caccttttgg tggaacattt acaacagcac
     3961 cctctataat tttaagaagt gcttgctgaa ctccctctcc tgaaacatct cttgtgatgc
     4021 ttctattttc actcattcta gcaattttat caatctcatc aataaaaatt attcctcttt
     4081 gagctttttc tatatctcca ccagcagctt gaacaagtct tgttacaaca ttttcaacat
     4141 catcaccaac atatccagct tctgttaaac ttgttgcatc tgcaatagca agaggaacat
     4201 caagatattt tgatattgtt tgagccaaaa gagtttttcc gcttccagtt ggtccaatta
     4261 aaagtacatt tgatttattt aattcaatat caccttgtaa ttcatctttt ctaaaaattc
     4321 ttttataatg gttgtaaaca gcaacagaaa gaaccttttt agctctttct tgccctataa
     4381 catactcatc taaaatcttt tttaactcag caggagtttt tatttttata ttttctactt
     4441 tttcaggttt agattttgtt tcaccatcaa caaaatctcc actcataata tcatacgcag
     4501 caccaacaca agctttacat atacaagcat tatctcctgt tattatagga tttgcttttg
     4561 tgtcagaagc tccacaaaaa tcacatatta atttactcat tttcgctctt tatttttctt
     4621 tcaaaaggaa tacctctttt tgtatttaat acaaaatttg ccatctcttt tacataagga
     4681 ttcttatcat tttcatataa atcaagagca gcttcttgaa gaggtcttct catgttaaat
     4741 agttctctat atgcatgttt taaagcatct acatcttctc tattttctag tcttcttcta
     4801 agacctacaa gatttaatcc ttttactaaa gctttattcc cttcagctaa acaaaatggt
     4861 ggaatatctt gtgcaactgc gctagctcct cctaccataa ctcctgttcc aattttacaa
     4921 aattggtgaa ttggagtaag acctccaata actacaaaat ctccacactc aacatgacca
     4981 gccaaagtta caccatttgc aaaaattgag ttattcccaa taacacaatc atgagccaca
     5041 tgaacgtaat tcattaacaa attacagttt cctat
//
LOCUS       DAOQAN010000018         5018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_21160_length_5018_cov_3.280073, whole genome shotgun sequence.
ACCESSION   DAOQAN010000018 DAOQAN010000000
VERSION     DAOQAN010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 5018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5018
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21160_length_5018_cov_3.280073"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(55..1259)
                     /gene="trpB"
                     /locus_tag="PKO10_00580"
                     /pseudo
     CDS             complement(55..1259)
                     /gene="trpB"
                     /locus_tag="PKO10_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014769921.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
     gene            1323..2615
                     /locus_tag="PKO10_00585"
     CDS             1323..2615
                     /locus_tag="PKO10_00585"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HNW65689.1"
                     /translation="MKIVFFGVGAVCSVMATLLDELSNKSKQKDINFVFVVRNIKKAQ
                     GHFFKNSNILNKSDFLEIKHFEEIFEDSKKYEKYLQNSDIFINSSTPSFNLPIMNLAL
                     KYNTNYADLASDIYKDEIISTLKFDQQILENEFKSQNLFALINLGISPGITNFLIGEK
                     IHSLKNLPYEVKIKKLEINLLEEIQSKKLIFSWSPKVAIDELAFPPVFYKKNRPKTIE
                     PFSKSKVYKFPYFRNIVDIYPVFQEELISLKQSFPQIENLKLFVGGSEIELMKNLYQL
                     NLFSNKYCYENSDSKISINTIIKNIVPKMKTPEIVEDYIKKKTIKYAEFSAVADIYLE
                     ILYPQNDKRISTIESVGLSFNKYTELVKTAYSGSTYVSYPTGVAAGILIYYSLLNKKR
                     LSGVILSENLPELFEHTVNDIIKRELGNYKINLINQIK"
     gene            2686..4002
                     /locus_tag="PKO10_00590"
     CDS             2686..4002
                     /locus_tag="PKO10_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013459920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNW65690.1"
                     /translation="MQKDIFRPFLNENSDKLNFLRYNTIGKNKDKYFDFTASGLAFRQ
                     IENRIHEVLETYANTHSKESSNAKITTKYYEDARQTLQKSLEIDDSFTILPCGSGATG
                     AIKKLQEILGVYIPPKTKERLKEKGLKIDKKSMPLVIVGPYEHHSNEVSFRESLAQVK
                     RVKLRKDGLIDLEHLENILEKNKNREIIGSFTIASNVSGIITCYKKISNLLRKYKATV
                     CFDAAASSSYMNISSSYYDALFLSPHKLLGGVGSCGILVVKKYLVDTSLPPSFAGGGT
                     VKYVNKSYQIYEEQIEVREDAGTPAITQFIRAALAYKLRNEIGFEFIENRKKELYLYL
                     LEELKSIQNLLIYGNKKSHNIGIVSFNIKDFNPYTLCEKLSNSGFQTRAGCSCAGPYG
                     HDLLGLKKVSMKNRPGWLRVGVHYIQTKDEIKALVDEIKNSLKTIL"
     gene            complement(3992..4324)
                     /locus_tag="PKO10_00595"
     CDS             complement(3992..4324)
                     /locus_tag="PKO10_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018060786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HNW65691.1"
                     /translation="MVNYIPSKGDIVTLDFDPQKGHEQKGRRPALILSNKTFNKALGL
                     AFACPITSTKRDFPFHVKIQSQKISGFIMAEQLKSIDYNSRNIKFVEKVDKEILEEIE
                     AIVDAIIK"
     gene            complement(4318..4560)
                     /locus_tag="PKO10_00600"
     CDS             complement(4318..4560)
                     /locus_tag="PKO10_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018060785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HNW65692.1"
                     /translation="MTVTISKWGNSQGFRVPKDILDKLNLAVGDLVNISQKDNKLIIE
                     PAKKEKKYSIDELVNNLPKGYKASEEFSSKSGSEIW"
     gene            complement(4715..>5018)
                     /locus_tag="PKO10_00605"
     CDS             complement(4715..>5018)
                     /locus_tag="PKO10_00605"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNW65693.1"
                     /translation="DYLIPLNDKNKLYVGAHAGVSSFKGKDEISDYSMSGFAYGAQAG
                     YIYDITKNIEFEFGLNYTKHDLDKTGTMRANNGNPVSVKLEMKDSISTFAGINYKF"
BASE COUNT         1857 a          729 c          663 g         1769 t
ORIGIN      
        1 tgaagctaaa aaacaataaa taaatacaaa gtaggattaa ccctactttg tattctaatc
       61 tcttggatag ttatcgatta taaaatctat atccttatcc cctcttccac taagatttac
      121 caaaattgat tttccactat tatcttttgc ccatttcata gcaaaagaaa cagcatgaga
      181 agattcaagt gctggaataa ttccttcaca tcttgaaagt gtataaaatg cttcaacagc
      241 ctcttcatca ctacaaagtc ccactttagt tcttccaatg ctatttaaat atgcatgttc
      301 aggaccaaca gatggataat ctatcccact tgctattgag tgaacaggag caggttctcc
      361 attttcatca attaacataa tagagttaaa tccatgcata actccctctt ttccataagt
      421 aatacttgca ctatgttcac ctaatttaga gccttttcca gctggttcta ttgcatataa
      481 atcaacacta tcttcaataa aagcactaaa tattcccata gcattacttc cacctccaac
      541 acaagcagct attgcatcag gaagttttcc agtcatctca aaaaattgct cttttgattc
      601 aataccaata atactttgaa aatctctaac catttttgga aatggatgag gaccaaccac
      661 acttcctatt gcaaacattt gagtttttgt atcttttaaa taactctcaa aagcactatc
      721 aactgcctct tttaaagttt ttaatccttt tgtaacaggt attatttagc acctaagatt
      781 ttcattttta caacatttgg atgctctttt ttaatatcaa cttcacccat atgtatttca
      841 cactctaatc caaaataagc agccgcagtt gctaacgcaa caccatgttg ccctgctcct
      901 gtttcagcta taactctttt tttacccaaa tattttgcaa gtaaaacctc tgccatacag
      961 tgatttagtt tatgagctcc tgtatgattc aaatcctctc tttttagata gattcttgca
     1021 ccccctacta aatttgataa attattacaa tatgtaattg gagttggtct tccttgataa
     1081 tattttctaa ttttttttag ctcatctata aattgaaaat catttgataa tttattataa
     1141 gcttcattta tctcattaaa tggaagttca agttgtggtg gaataaaaga tcctccaaat
     1201 ttaccaaaat atccattttc atctggaaaa ctttgcaaat atggcttttg tttctgcatt
     1261 taagtacctt taaaataaaa tacaaaaata ttatcatatt ttaaataatt ttaggggtac
     1321 atatgaagat agtttttttt ggtgttggcg ctgtttgtag tgttatggca actttgttag
     1381 atgaactttc taataaaagc aaacaaaaag atatcaattt tgtatttgtt gtaagaaata
     1441 taaagaaagc tcaaggtcat ttttttaaaa atagtaatat tttaaataaa tcagattttt
     1501 tggagataaa acattttgaa gaaatttttg aagatagtaa aaaatatgaa aaatatctac
     1561 aaaatagtga tatttttata aatagttcca ctccatcttt taatctacct attatgaatt
     1621 tagctttgaa atacaataca aattatgctg atttggcaag tgatatttat aaagatgaga
     1681 taatttcgac acttaaattt gatcaacaaa ttttagaaaa tgagtttaaa tcgcaaaatt
     1741 tatttgcttt aataaatctt gggatttctc ctggaattac aaacttttta atcggtgaaa
     1801 aaatacactc tttaaaaaat cttccttatg aagttaagat taaaaaatta gaaatcaacc
     1861 ttttagaaga gattcaatca aaaaaattaa tcttttcgtg gtcaccaaaa gtagctattg
     1921 atgaacttgc atttcctcca gttttttata aaaagaatag accaaaaact attgaaccat
     1981 tttcaaaatc aaaagtatat aaattcccat attttagaaa tattgtcgat atttatccag
     2041 tttttcaaga ggagttaata tcactaaaac aaagttttcc tcaaattgaa aatttaaaac
     2101 tctttgttgg tggaagtgaa attgaattaa tgaaaaattt atatcagcta aatttattct
     2161 caaataaata ttgctatgaa aacagtgatt caaaaatatc gattaatact attataaaaa
     2221 atatagtccc aaaaatgaaa actcctgaaa ttgttgaaga ttatataaag aaaaaaacaa
     2281 tcaaatatgc tgaattttca gcagttgctg atatatattt agaaatttta tatcctcaaa
     2341 atgataaaag aataagtact atagaatcag ttggtctatc ttttaacaaa tatactgaac
     2401 ttgtaaaaac agcatattca ggttcaactt atgtatctta tccaacagga gttgcagctg
     2461 gaattttaat ttactactca ttattaaata aaaaaaggct aagcggagta attttaagtg
     2521 aaaaccttcc agaacttttt gaacatactg taaatgatat tataaaaaga gagttaggaa
     2581 actataaaat aaatctaata aaccaaatta aatagttcta aaatataaat aagataaaat
     2641 tatggtttat ataaatatta taaataattt taaaagaaga gttttatgca aaaagatatt
     2701 tttagaccat ttttaaatga aaatagtgat aaacttaatt ttcttagata taacacaata
     2761 ggtaaaaaca aagataaata ttttgatttt acagcctcag gtcttgcttt tagacaaatt
     2821 gaaaatagaa tacatgaagt tttagaaact tatgcaaata cacactcaaa agagagtagt
     2881 aatgcaaaaa ttactactaa atattatgag gatgctagac aaactttaca aaaaagttta
     2941 gaaattgatg atagctttac tattttacct tgtggttcag gagcaactgg tgctataaaa
     3001 aaactacaag agattttagg agtttatatt ccaccaaaaa caaaagagag gctaaaagaa
     3061 aaaggtctaa aaatagataa aaagtctatg cctttggtaa ttgttggacc ctatgaacat
     3121 cactcaaatg aggttagttt tagagagagt ttggcacaag ttaaaagagt gaaacttaga
     3181 aaagatggtc taatagacct tgaacactta gaaaatatat tagaaaaaaa taaaaatcgt
     3241 gagattatag gctcttttac aatagcttca aatgtaagtg gaataataac ttgctacaaa
     3301 aaaatatcaa atcttttgag aaaatataag gcaactgtct gttttgatgc agctgcaagt
     3361 agttcgtaca tgaatatttc atcatcatat tatgatgcac tatttttatc tcctcataaa
     3421 cttttaggag gtgttggatc ttgtggaatt ttagtagtta aaaaatattt agtagataca
     3481 tctttaccac caagttttgc aggtggtgga acggtaaaat atgtaaataa aagttatcaa
     3541 atatatgaag aacaaataga agtaagagaa gatgctggaa ctccagcaat tacacaattc
     3601 ataagagctg ctttggcata taagttaaga aatgaaatag gatttgaatt tatagaaaat
     3661 agaaagaaag agttatattt atatctatta gaagagctaa agagtattca aaatctactt
     3721 atttatggaa ataaaaagag ccataatatt ggtattgtat cttttaatat aaaagatttt
     3781 aatccttaca cattgtgtga aaaactttca aatagtggtt tccaaactag agctggttgt
     3841 tcttgtgctg gaccttatgg gcatgattta ctaggactta aaaaagtatc tatgaaaaat
     3901 cgaccaggtt ggcttagagt tggagttcac tatattcaaa caaaagatga aattaaagct
     3961 ttggttgatg agattaaaaa tagtttaaaa actattttat aattgcatct actatagctt
     4021 caatctcttc tagtatctcc ttatctactt tttcaacaaa ttttatattt cttgagttat
     4081 aatcaataga ttttaactgt tcagccataa taaaaccaga aattttttgg ctttggattt
     4141 ttacatgaaa agggaaatcc ctttttgtac ttgtaattgg acaagcaaat gctaaaccta
     4201 aagctttatt aaaagtttta ttacttaaaa tcaaagctgg tcttcttcct ttttgctcgt
     4261 gtcccttttg tggatcaaaa tctaatgtta caatatcacc tttgcttgga atataattta
     4321 ccatatttca gaaccactct tagagctaaa ctcttcacta gctttgtaac cttttggtag
     4381 attattcaca agctcatcta tactatattt tttctctttt tttgctggtt ctattattaa
     4441 tttattatct ttttgagaga tatttaccaa atcaccaact gctaaattta acttatctaa
     4501 tatatctttt ggaactctaa agccttgaga gtttccccat tttgaaatag ttactgtcat
     4561 aaaaaacctt tttgaaaata tttttttgta tatacgaagt ttagccaaag agggttgaaa
     4621 aagtcaatat atttttgaac ttaaaaataa atcatttaaa agataagcaa gagagtgctt
     4681 atcttttttt tcgaaatttt aaagaagatt aaaattagaa tttgtagtta attcccgcaa
     4741 atgtagatat tgaatctttc atttcaagtt ttactgatac aggattacca ttgttagctc
     4801 tcattgttcc agttttatct aaatcatgtt ttgtataatt taaaccaaat tcaaactcta
     4861 tattttttgt aatatcataa atataaccag cttgtgctcc atatgcaaat cctgacatac
     4921 tataatcact tatttcatct ttacctttga agctagaaac accagcatga gcacctacat
     4981 ataatttatt tttatcattt aatggaatta aataatca
//
LOCUS       DAOQAN010000019         4934 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_21722_length_4934_cov_3.744620, whole genome shotgun sequence.
ACCESSION   DAOQAN010000019 DAOQAN010000000
VERSION     DAOQAN010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 4934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4934
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21722_length_4934_cov_3.744620"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>4934)
                     /locus_tag="PKO10_00610"
     CDS             complement(<1..>4934)
                     /locus_tag="PKO10_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW65694.1"
                     /translation="NGNLVATETGPKSFRLYEPPTKNLPINNEMVQQIASQEGITLPK
                     NYDPSLPTYFKTIKNIKGNYDVSLMQIKPSYLRTWANYLKVKTTPAYDYVIKDLGSSG
                     RDFNRAIESGDKPVTDIYKNTEGVLQPNVAKQTVNDLALKLDNVKPGLGEQFKNSVKL
                     DGATPTSLIEQATAIYDKSLGINLPPDKVIFNPIISGEIKVPDIKSKVDLIPETTQTK
                     QNLIEKTNQLITKLPAAVANKDAQVVSLAQQYALETGDAELINKVNQIIGENQKTNKG
                     IEQMMEQSVQTPEKQLTQPAFTPYKSARKPTINSVYGNTPSDKFIVGTYDGKPYTTNA
                     YILEFNSDVTPPAKALLFNEEVGANKVPDETAIEKLIPKETAPVELVKVYGEEGGKME
                     LATLEGDNGTLNIQRKYYDYFNNKYKNPQFFAGKQGPILVMKGDQKVGLIMPMANDIS
                     IKKPVTVWEKPVTAQPELKQQEVVEKKTKEKEKAIVPNADKPLSKEELEALPKEDRKD
                     YELYLKDKIEALPKLKEGEITVIFGGNAKSQYVDTNIQIALNRGVNANTNVMNVLEED
                     LKIPNAERAERGERIFEMKEGANKTPLEKAKDARTLAVLQERVMFEGKMMSKEDIFSK
                     LMAEGYETKMEVTKDFKKIQKLQDQYDDASNVAYGIKGRSPREKLKAELDEAKAKEIT
                     TYKLTNGKQSYPLKKLEYDALQIKEWEVGGSKYEKLRNEMNTPEDIALHKKAGLEETG
                     IEFVGPIRWNGTQEQWSKYAELKAKANTKQVTESLSEDGLGSPVGSKTIRRKVEEQNY
                     KLNMGEVQEVPEDFKISQRAKDILAEFGVVINEKQVPNKYLGFYKYITKKVRVQALYD
                     VTTVTHEAVHAIDAQISFSKKLIEDSGRGADVRKQLTDIYEDIYPGAKREHPLDKRIK
                     EGLAVLFENYFYDPASISVEYPDLVNSFIKPEGKYYDPLFTKLLERMNQLVDDYARLT
                     PEQRIGSRIRTGKEVVDNDTGFTWKQRLEYEIFNKYEPLKRYGKMAGVEGTWNDPLIQ
                     SFNMMNKNAIAASWVKGDITPVLMANGNFKLEKGTVKDYLEIIKKLDLFDEGQPIPKE
                     ATDTMTGAKGVFKAAVKKISDVHGYSPFKEYNLKTYRSYLVARRVVADNNKVRMLENT
                     ARELEKFVSGFMGEDFAEIAGELDEIKALNSQIAKLKEIISRDDFSLQDASAVVEKYA
                     DKFKEADAIYDGINRKMIEIAVQNELIPQATADEMIAENGYTSFRRYIDEDLNAVGTI
                     RTSSKSKVSAFKERSGSQLDIVDPIYSQMQSINEIIGKSMENRLWLKVADLTRGNPDI
                     ARRFEKIAAVPSIAEDGTISYPQEKQEGVIRVFRNGKREFYKVGAEFQAVAKTMRPKE
                     LDLFSKIIRIPSSLFTRLTTSANPLFAVGNLSVDQFSALSQTKTGFKPIVDPAKSFVD
                     YIKGTDGINAYIALGGKRQTLASFYDLSPEQIEHKLTGGLSTYEKAAGIVDYGISILE
                     MPSNTSEIMTRYSEFKRAKDQGDSDSVAMYKASEVTTPFQLMGNLGGQFGQAYIKSIP
                     YLNAQLQVLYKWGRASKDNPKRMGTVMAGLLTAGLTAAILAMKYGTEEQKRAIGEQPV
                     RNMSRYIYIPAPNGKDLIKFRIPEIMGVFTGMGY"
BASE COUNT         1292 a          919 c          707 g         2016 t
ORIGIN      
        1 ataacccatt ccagtaaaga cacccattat ttctggtatt ctaaacttta ttaaatcttt
       61 accatttgga gctggaatat atatgtatcg tgacatattt ctaactggtt gttctccaat
      121 agctcttttt tgttcttctg ttccatactt catggctaaa atagcagcag ttaatcctgc
      181 agtaagtaac cctgccataa ctgttcccat tctctttgga ttatctttac ttgctcttcc
      241 ccacttatat agaacttgta attgagcatt taagtaagga atactcttta tatatgcctg
      301 tccaaattga cctccaagat tacccataag ttggaatgga gttgtaacct ctgaagcttt
      361 atacatagct acagaatctg aatctccttg atcctttgct cttttaaact ctgaatatct
      421 agtcattatt tctgaagtat ttgaaggcat ttccagaata ctaataccat aatcaacaat
      481 tcctgcagct ttttcatatg ttgaaagacc accagtaagt ttgtgttcta tttgttctgg
      541 tgataaatcg tagaatgaag caagtgtttg ccttttacca ccaagagcta tataagcatt
      601 tattccatct gttcctttaa tataatcaac aaagctttta gctgggtcaa ctattggttt
      661 aaaacctgtc tttgtttgag atagagcaga gaattggtca actgataagt ttccaactgc
      721 gaatagtgga tttgctgaag tcgttaaacg agtaaataat gatgatggta ttcttattat
      781 tttagagaat aagtctaatt cctttggtcg cattgtcttt gcaactgctt ggaattcagc
      841 acctacttta tagaactctc tctttccatt tctaaatact ctgattactc cttcttgttt
      901 ttcttgtgga tatgagatag tgccatcttc agctattgat ggaactgcag caattttttc
      961 gaatcgtctt gcaatatctg gattacctct tgttaaatca gcaactttta accatagtct
     1021 attctccata gacttaccta taatttcatt tattgactgc atttgtgagt aaattggatc
     1081 aacaatgtcc aattgactac cacttctttc cttgaatgca cttactttgc ttttacttga
     1141 agtccttatt gttcctacag cattaaggtc ctcgtcaata tatcttctga atgaggtata
     1201 accattctct gctatcattt catctgctgt ggcttgtgga ataagttcat tttgaactgc
     1261 tatttctatc atcttcctat ttattccatc atatatagca tcagcttcct tgaacttgtc
     1321 tgcatatttt tccacaactg cacttgcatc ttgaagagag aaatcatctc gtgaaataat
     1381 ttctttaagt ttagctattt gtgaatttag tgcttttatt tcatctagtt ctcctgctat
     1441 ttctgcaaaa tcttcaccca taaatccact aacaaatttt tcaagttctc gtgctgtatt
     1501 ctcgagcatt ctaactttgt tattatcagc aacaactctt cgagctacaa gataacttct
     1561 atatgttttt aaattatatt ccttaaatgg agaataacca tgaacatctg aaatcttttt
     1621 tactgctgct ttaaatacac cctttgcacc ggtcattgta tcagtagctt cttttggtat
     1681 aggttgtcct tcatcaaaca aatcaagctt cttaattatt tccaagtagt ctttaactgt
     1741 tcctttctca agtttaaagt taccatttgc cataagaact ggagttatgt cacccttaac
     1801 ccaacttgca gcaatagcat ttttgttcat catattaaat gactggataa gtgggtcatt
     1861 ccaagttcct tcaactcctg ccatttttcc atatctcttc aatggttcat atttattgaa
     1921 tatctcatac tctaatcttt gcttccaagt aaaaccagta tcattatcaa cgacttcttt
     1981 accagttcta attcttgaac ctattctttg ttctggtgta agtcttgcat agtcgtcaac
     2041 taattggttc attctttcca aaagtttagt gaatagtgga tcatagtatt ttccttctgg
     2101 tttaatgaaa ctattaacta ggtctgggta ttcaacacta attgaagctg ggtcgtagaa
     2161 gtagttttca aatagaactg ctagtccttc tttaattctt ttatctagtg ggtgttccct
     2221 tttagctcca ggatagatgt cttcataaat atctgtaagt tgctttctga catctgcacc
     2281 tcttccagaa tcttctatca attttttaga gaaacttatc tgtgcatcaa tagcatggac
     2341 tgcttcatga gttactgtag tgacatcata taaagcttga actctaactt ttttagttat
     2401 gtatttgtaa aaaccaagat atttattagg aacttgtttt tcatttatta caaccccaaa
     2461 ttctgcaagg atgtctttgg ctctctgtga tattttaaag tcctctggaa cttcttgaac
     2521 ttcacccata ttgagtttat agttctgttc ctcaactttt ctacgaatag ttttacttcc
     2581 gactggtgaa ccaagaccat cttcggaaag agattcagtt acctgtttag tgtttgcttt
     2641 tgcttttaat tcagcatatt tagaccattg ttcttgagtt ccattccatc ttattggtcc
     2701 aacaaactca atacctgttt cttctaatcc agctttttta tgtaatgcaa tatcttctgg
     2761 agtattcatt tcatttctta atttttcgta ttttgaacct cctacttccc attcttttat
     2821 ttgtagtgca tcatattcta atttctttaa tgggtatgat tgtttaccat tagtaagttt
     2881 atatgtagtt atttcttttg ctttagcttc atcaagttct gctttcaatt tttctcttgg
     2941 tgatcttcct ttaattccat aagcgacatt tgaagcatca tcatactggt cttgaagctt
     3001 ttgtattttc ttaaaatctt tagtaacttc catttttgtt tcataccctt ctgccataag
     3061 tttactaaaa atatcttctt tggacatcat ctttccttca aacattactc tttcctgtaa
     3121 tacagctaat gttctagcat ctttggcttt ttctaatggt gttttattag ctccttcttt
     3181 catctcaaat attctttctc ctctttcagc tcgttcagca tttggaatct tcaaatcttc
     3241 ttctaggaca ttcattacat tcgtattcgc atttactcca cgattaagag caatctggat
     3301 attagtatca acatactggc tcttggcatt tccaccaaaa ataacagtaa tctccccttc
     3361 ttttaattta ggtagtgctt cgatcttgtc cttaagataa agttcataat cttttctgtc
     3421 ttcttttggt aatgcttcaa gttcttcttt agataatggt ttatcagcat tcggaacgat
     3481 agctttctct ttctctttcg tctttttctc tacaacttct tgttgtttta gttctggttg
     3541 tgcagttact ggtttctccc aaacagttac tggttttttg atacttatat catttgccat
     3601 tggcattata agtccaacct tttgatcacc tttcattacc aaaataggtc cttgctttcc
     3661 tgcaaagaat tgtggatttt tatatttatt attgaaatag tcgtagtatt ttctttgaat
     3721 atttagagtt ccattatctc cttcaagtgt agcaagttcc atttttccac cttcttcacc
     3781 atatactttt acaagttcta ctggtgctgt ttcttttgga ataagttttt caatagctgt
     3841 ttcatctgga actttatttg ctccaacttc ttcattaaat agaagtgctt ttgctggtgg
     3901 agttacatca ctattgaatt ccaagatgta agcattagtt gtatatggtt ttccatcata
     3961 agttccaacg ataaatttat cgcttggagt attcccataa acagaattaa ttgttggttt
     4021 tcttgctgac ttatatggtg taaatgctgg ttgagtaagt tgcttttctg gtgtctgaac
     4081 tgactgttcc atcatttgtt ctataccttt atttgtcttt tgattttcac ctattatctg
     4141 atttactttg tttattagtt ctgcatcacc agtttccaaa gcatattgtt gggcaagtga
     4201 aacaacctgt gcatctttat ttgcaactgc tgctggaagt tttgtaatga gttgatttgt
     4261 cttttctatt aaattctgtt ttgtttgagt tgtttctggg atcaaatcaa ctttactttt
     4321 tatatctgga actttaattt caccactaat aattggatta aatattactt tatctggtgg
     4381 taaattaata ccaagagatt tatcataaat tgctgtggct tgttcaataa gacttgttgg
     4441 tgtagcacca tcaagtttta ctgaattttt aaattgttca ccaagtcctg gtttaacatt
     4501 atcaagtttt agagctaaat catttacagt ttgttttgct acatttggtt gtaatactcc
     4561 ttctgtattt ttataaatat cagttactgg tttatctcca gattcaatag ctctattaaa
     4621 atctcttcca gaagaaccta aatcttttat tacataatca tatgctggtg tagtttttac
     4681 ttttagataa ttagcccaag ttcgtagata tgatggttta atttgcatta aactaacatc
     4741 ataatttcct tttatgtttt ttatagtttt aaaatatgta ggtaaagaag gatcataatt
     4801 tttgggtaaa gtaattcctt cttgacttgc tatttgttga accatttcat tatttatagg
     4861 taaatttttt gttggtggtt catataatct aaaactttta ggacctgttt ctgttgcaac
     4921 taaatttcca ttaa
//
LOCUS       DAOQAN010000020         4899 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_21942_length_4899_cov_2.658960, whole genome shotgun sequence.
ACCESSION   DAOQAN010000020 DAOQAN010000000
VERSION     DAOQAN010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 4899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4899
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21942_length_4899_cov_2.658960"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..860)
                     /locus_tag="PKO10_00615"
     CDS             complement(<1..860)
                     /locus_tag="PKO10_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014516708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNW65695.1"
                     /translation="MARKVPLNRVRNIGIAAHIDAGKTTSTERILFYTGVSHKIGEVH
                     EGAATMDWMEQEQERGITITSAATTCFWTHPKTNEQLQINIIDTPGHVDFTIEVERSM
                     RVLDGAVAVFCSVGGVQPQSETVWRQANKYGVPRIIYVNKMDRTGANFFNVMNQVRDR
                     LKANPVPLQIPIGAEDQFRGMVDLVKMKAYTYNLDAQAGEMYKIEDIPADLVDTANEY
                     REKLVEAAAESSDELMDKYLGGEELTEEEITSGIKKRCLAMEVTPMVCGTSFKNKGIQ
                     PLLDAVAMYLP"
     gene            complement(872..1339)
                     /gene="rpsG"
                     /locus_tag="PKO10_00620"
     CDS             complement(872..1339)
                     /gene="rpsG"
                     /locus_tag="PKO10_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HNW65696.1"
                     /translation="MRRRKAPVREIMADPIYNSKVITKFVNAIMLDGKKSVAEKILYG
                     AIDNLDKRGEEKGFDLFEKAVENVKPLLEVRSRRVGGATYQVPVEVRAVRRQTLALRW
                     LIDASRKRNERTMVERLANELFEAANERGASFKKKEDVHRMAEANKAFAHYRW"
     gene            complement(1355..1735)
                     /gene="rpsL"
                     /locus_tag="PKO10_00625"
     CDS             complement(1355..1735)
                     /gene="rpsL"
                     /locus_tag="PKO10_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011371990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HNW65697.1"
                     /translation="MPTIQQLVRKERKKVVEKSKSPALKKCPQRRGVCTRVYTTTPKK
                     PNSALRKVAKVRLTTGFEVISYIGGEGHNLQEHSIVLVRGGRVKDLPGVKYHIVRGAL
                     DSAGVNNRTVSRSKYGTKRPKAKK"
     gene            1944..2186
                     /locus_tag="PKO10_00630"
     CDS             1944..2186
                     /locus_tag="PKO10_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNW65698.1"
                     /translation="MTILNYKKFGNGEKKLIFLHELMGDCTNYENCLIYFDKNLFTIF
                     MVDLRGYGLSKNILGKYNLDEAVNDVINLVTNFKID"
     gene            2179..2709
                     /locus_tag="PKO10_00635"
     CDS             2179..2709
                     /locus_tag="PKO10_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNW65699.1"
                     /translation="MTDYVLVAHSMSSMIAQHITSKDKRVKKLILLTPISYKGVKSTQ
                     KAKDNLLTQMEKNSGKIEDIVEQSSKRYNQIWKDYRINLAYNSSLLEARISYMNMYLN
                     IDYESNFEKLEINIPIKIITGKYDFPVFSKNEVAKYFEEFNDVEIIEFEEAGHYPMIE
                     SPVLFASKIEFWVKNL"
     gene            2706..3860
                     /locus_tag="PKO10_00640"
     CDS             2706..3860
                     /locus_tag="PKO10_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014475128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNW65700.1"
                     /translation="MTKQIFPLALGGLAIGTTEFIIMGLLPDVAKDLGVSIPIAGHLI
                     SAYAFGVVVGAPILVALSSKFPPKNILIVYMILFTLFNALSIIAPDYNTLLMSRFLAG
                     LPHGAFFGVGTIVAIKLSKKGKEAQAVSSMFTGLTLAILLLVPLLTYIGHNTSYKWAF
                     LIVSLLGIFTILALIKFMPKLKSLKTVTFKEEIEFFLTFKAWHIILIVAIGFGGLFAW
                     FSYIAPLLINVSKFPPSSISYLMLVAGVGMVVGNILGGYLADKKDPIKVAIYLLSFMV
                     IFLILVFFLSENKILSIILTFICGVFAMSVGTPINMVMVKSAKNSEMLGAAFMQAAFN
                     VANSLGALFGGIPLLYGLGFEYPALFGAFMAFLGLLLCMLFYIKYSKEKI"
     gene            complement(3893..4633)
                     /locus_tag="PKO10_00645"
     CDS             complement(3893..4633)
                     /locus_tag="PKO10_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012148082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HNW65701.1"
                     /translation="MKKVILGISIIFCVTLSGRDNPFAKYEEETGKMYELNETPKTVE
                     EIQEAQYIQKIQNQINSQSEPVTSAPKANVQKAPEKTYTKKELEAIVQKANKQNEQKT
                     KEIVKKELANVKKEPEQVIYVKPRADVDSDKTSTTVNPSSNSLKQKNILPFLNIENSD
                     NELIIRSEHKMFKKFSIDKENKLAFDYKAKVNFNTKKEILAGNNFKNITVGNHQKGGY
                     YRVAVELGSKVSKYSVDVNDKEIVIKLK"
     gene            complement(4630..4839)
                     /locus_tag="PKO10_00650"
     CDS             complement(4630..4839)
                     /locus_tag="PKO10_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014475394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator"
                     /protein_id="HNW65702.1"
                     /translation="MLVVIFSLLATVYFAYHWVNLLFGDNSIQVYNSLKHKKEYLEDE
                     ISRLQKENAYLQKEYFELKNLEPEE"
BASE COUNT         1487 a          801 c          661 g         1950 t
ORIGIN      
        1 ggtaaataca tagcaacagc atcaagtaat ggttgaatac ctttattttt aaatgatgtt
       61 ccacaaacca ttggagttac ttccatagct aaacatcttt ttttgattcc agaagtaatc
      121 tcttcttcag ttaattcctc accacctaag tatttatcca ttaactcatc acttgactca
      181 gcagcagctt caactaattt ttctctatac tcattagcag tatcaactaa atcagcagga
      241 atatcttcaa ttttatacat ctctccagct tgtgcatcca gattatatgt ataagctttc
      301 atttttacta aatcaaccat tcctctaaat tggtcttctg caccaattgg aatttgaagt
      361 ggaactggat tagcttttaa tctatctctt acttgattca taacattaaa gaagtttgca
      421 cctgttctat ccattttatt tacatagata attcttggaa ctccatactt atttgcttgt
      481 ctccaaacag tttcagattg tggttgaact cctccaaccg aacaaaatac tgcaacagca
      541 ccatcaagaa ccctcataga tctctcaact tcaattgtaa agtcaacgtg acctggagtg
      601 tcaatgatat ttatttgtag ttgctcattt gtttttgggt gtgtccagaa acaagtagta
      661 gcagcagaag taatagtaat acctctttct tgctcttgct ccatccagtc cattgttgca
      721 gcaccttcat gaacttctcc aattttgtga gaaactcctg tatagaataa aattctctct
      781 gtacttgtag tttttcctgc atcaatgtgt gcagcaatac caatatttct aactctgttt
      841 aaaggtactt ttcttgccat aataatttat cctaccatct gtagtgtgca aatgctttgt
      901 tagcctctgc cattctatgt acatcttctt ttttcttaaa agatgctcct ctttcgtttg
      961 ctgcttcgaa tagctcattt gctaatctct caaccattgt tctttcattt ctttttcttg
     1021 aagcatcaat aagccatcta agtgctaaag tttgtcttct tacagctcta acttcaacag
     1081 gaacttgata tgtagctcct ccaactcttc tacttcttac ttctaaaagt ggtttaacat
     1141 tttcaactgc tttttcaaac aggtcaaaac ctttttcttc acctctttta tcaaggttat
     1201 ctattgcacc ataaaggatt ttttcagcaa cagatttttt accatctagc ataattgcat
     1261 taacaaattt tgtgatcact ttactattgt agataggatc agccattatc tctctaactg
     1321 gagcttttct tcttctcatt ttctatcctt ctacttattt tttagctttt ggtctttttg
     1381 taccatactt agatctagat acagttctgt tatttacacc agctgaatct aacgcacctc
     1441 ttacaatgtg gtatttaacc ccaggtaaat cttttactct tccccctctt actaacacaa
     1501 ttgagtgttc ttgaaggtta tgtccctctc caccgatgta tgaaataact tcaaatccag
     1561 ttgttaatct aacttttgca acttttctta aagccgagtt aggttttttt ggagttgttg
     1621 tatatactct tgtacatact cctcttcttt gtggacattt tttaagtgct ggagattttg
     1681 atttctcaac cacttttttt cgctcttttc ttacaagctg ttgtattgta ggcatttctt
     1741 tcctttataa tatttggttt gagtagttat agccccactc atgcttctcg taatcttcaa
     1801 aatactttga agcgatacta ccattctagg aaactgttgt agtgttttct aaaaaagttt
     1861 tgcattatac ctaaatgctc ttaaaatttt ctttattaca ttattatcaa ttttataata
     1921 gaattttttt aaaggcaaaa ataatgacaa tattaaacta caaaaaattt ggaaatggtg
     1981 aaaaaaaact tattttctta catgaattaa tgggcgattg tacaaattat gaaaattgtt
     2041 taatctactt tgataaaaat ctttttacaa ttttcatggt tgatttaaga ggatatggat
     2101 tatcaaaaaa tattttagga aaatataatt tagatgaagc tgtaaacgat gtaataaatt
     2161 tagtaacaaa ttttaaaatt gactgattat gttttagtcg ctcactctat gtcttcaatg
     2221 atagctcaac atataacttc aaaagataaa agagttaaaa aactcatttt actaactcca
     2281 atatcgtata aaggagtaaa aagtacacaa aaagctaaag ataatttact aactcaaatg
     2341 gaaaaaaata gtggtaaaat agaagatata gtagaacaat ctagcaaaag atataatcaa
     2401 atttggaaag attatcgtat aaatttagct tacaactctt ctcttttgga agctagaatt
     2461 tcttatatga atatgtattt aaatatagat tatgaaagta attttgaaaa gcttgaaatt
     2521 aatatcccta ttaaaatcat cactggaaaa tatgattttc cagttttctc aaaaaatgaa
     2581 gtagcaaaat attttgaaga gtttaacgat gttgagatta tagaatttga agaggcggga
     2641 cactatccta tgattgagtc tccagttctt tttgcttcaa aaattgaatt ttgggtaaag
     2701 aatctatgac aaaacaaatc tttcctcttg ctttaggtgg attagcaatt ggaacaactg
     2761 aatttattat tatgggactt ttaccagatg tcgcaaaaga tttaggagtt tctattccaa
     2821 ttgctggaca tttaatatct gcttatgctt ttggtgttgt agttggagct ccaatcttag
     2881 tagctttaag ttcaaaattt ccaccaaaaa atattttaat agtttatatg attttattta
     2941 ctctatttaa tgctttatct ataattgctc ctgattacaa tactctttta atgtcaagat
     3001 ttcttgcagg tcttcctcat ggtgcttttt ttggagttgg aacaattgtt gcaataaagt
     3061 tatcaaaaaa gggtaaagaa gctcaagctg tctcttctat gtttacaggt cttacattag
     3121 caattttatt attggtacca cttttaacat atataggaca caatactagc tacaaatggg
     3181 cttttttaat tgtttcactt ttaggtatat ttactatttt agctctaatc aaatttatgc
     3241 caaaactaaa atcacttaaa acagtaactt ttaaagagga gatagagttt tttctcactt
     3301 ttaaagcttg gcatattatt ttaatagttg ctattggatt tggaggactt tttgcatggt
     3361 ttagttatat tgcacctctt ttaatcaatg tttcaaaatt tcctcctagt tcaatttctt
     3421 atttgatgct tgttgctgga gttggaatgg ttgttggaaa tattttaggt ggatatttag
     3481 cagataaaaa agacccaata aaagtagcta tttatctttt aagttttatg gtaatttttt
     3541 taatcttggt atttttctta tctgaaaata agattttatc tattatttta acttttattt
     3601 gtggtgtttt tgctatgagt gtggggactc caataaatat ggtaatggtt aaaagtgcaa
     3661 aaaactctga aatgctagga gctgctttta tgcaagctgc ttttaatgta gcaaactctt
     3721 taggagcttt atttggagga attccacttt tgtatggatt aggttttgaa tatcctgcac
     3781 tatttggtgc ttttatggca tttcttggac ttttgttatg tatgcttttt tatataaaat
     3841 attcaaaaga gaagatataa tcttctcttt taataaatat aaattaaaaa aactatttta
     3901 atttaattac aatctcttta tcattaacat caacactata ctttgatact ttagaaccaa
     3961 gctcaacagc aactctataa taaccacctt tttgatgatt tccaactgta atattcttaa
     4021 aattatttcc tgctaaaatc tctttttttg tattaaaatt tactttagct ttataatcga
     4081 aagctaattt attctcttta tctatagaaa attttttaaa cattttatgt tcacttcgta
     4141 taatcaactc attatcacta ttttcaatat ttaaaaatgg caaaatgttt ttttgtttta
     4201 atgaatttga actaggattt acagtagtac ttgttttatc gctatctaca tcagctcttg
     4261 gctttacgta aattacctgc tctggctctt ttttaacatt tgcaagctct ttttttacaa
     4321 tctctttagt tttttgttca ttttgtttat ttgctttttg aacaatagct tctaactctt
     4381 ttttagtata agttttttct ggtgcttttt gtacattagc tttaggtgca gatgttactg
     4441 gttcactttg agaattgatt tgattttgta ttttttgaat atactgtgcc tcttgaatct
     4501 cttcaacagt ttttggagtt tcatttaact catacatttt gcctgtctct tcttcgtatt
     4561 ttgcaaatgg gttatctcta ccactaagtg ttacacaaaa tattatagat attcctaaaa
     4621 ttactttttt cattcttctg gctccaaatt ttttaattca aaatactctt tttgcaaata
     4681 agcattctct ttttgcaatc tcgaaatttc atcttctaag tactcttttt tatgctttaa
     4741 agagttataa acttgtattg aattatctcc aaaaagaaga ttcacccaat gataagcaaa
     4801 ataaacagta gctagtagtg aaaaaatcac tactagcata aatccaaaaa tattactttt
     4861 tttattagac attatttttt agagaaaggt tgtttccct
//
LOCUS       DAOQAN010000021         4837 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_22345_length_4837_cov_2.690506, whole genome shotgun sequence.
ACCESSION   DAOQAN010000021 DAOQAN010000000
VERSION     DAOQAN010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 4837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4837
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22345_length_4837_cov_2.690506"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="PKO10_00655"
     CDS             complement(<1..479)
                     /locus_tag="PKO10_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014475205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HNW65703.1"
                     /translation="MAQLSELELLKASRNPLRVIDDLYKEALEGIPLKDEYIGLLKWY
                     GMYPHINKDGLEDKKYFMKRIKLVDARMNLEQLRVMGEIGQKYAQGLVDFTTRQNVQF
                     HYIEIKDIPAIFDLLNSVNLTSRMASGDGPRPIMTCPVGGVDKNEIFDARELVKSVDA
                     "
     gene            676..1764
                     /locus_tag="PKO10_00660"
     CDS             676..1764
                     /locus_tag="PKO10_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014475206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M42"
                     /protein_id="HNW65704.1"
                     /translation="MSASFSNEPKDFTLFLDLLKQLIRVPSVTGAEHSFLLYLKRELE
                     EIGIKTEHYDGLLVASGKDPSNGILSAHIDRHGVISTGPNEFQFAAFLAKNRSDLRGN
                     SLSEQTFQQISQRYINQNVHAYEPFSGGYLGIGKITDAFLKEEINNLIFKIDGLNHLL
                     PGTPIAFVDKLKQNGNLISAQLDNVLSAAIILYLYQHGFAGTAFFTAQEEAGKSWRFV
                     HEWFKKNKISTDKLVVLDTSPYESREEADKQLIVLRNKDVNARFKSPLLKELKQFCKK
                     NKISFSCKDKFIIEKNKLREKDGLKPLSIGSTELGRIVMESNGQIQGATLQIPTTGYH
                     TVDETACVESIKAIIFILSSFYIKTVNI"
     gene            1785..2012
                     /locus_tag="PKO10_00665"
     CDS             1785..2012
                     /locus_tag="PKO10_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW65705.1"
                     /translation="MNLAIIYELRAADVKEEHIEQIMDKVKNRLSEENIDKELVKLGY
                     PKIFTVDYSEYDSFDYNDSFTPTSKASLKED"
     gene            2101..4215
                     /locus_tag="PKO10_00670"
     CDS             2101..4215
                     /locus_tag="PKO10_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tex-like N-terminal domain-containing protein"
                     /protein_id="HNW65706.1"
                     /translation="MNLIQLLQNKTNLPINIIENIIKLLDEGCTIPFIARYRKEFTNG
                     ASDSELRVFEEVFEYSKKLIQRKDEIKNLLKEKNFLDEKLIKFLDEATSLQALEDIYS
                     PFKDKKSSRTTTALENGLEPLANIIQSMRYTKEECEQKANQFLNENIKTINEAINGAK
                     DIIAQRYADDFKSKEVLRNLIQNWGVLEVTPTKEFDKNGLYSNFANINEKIKYIKSHR
                     VLAILRAVNEKQLSIKVEIDENHILENIKKYKIPTNASSSKELVFDGFKDGLKRLLLP
                     TLKREAISNLKEKAGSEAIELFGKNLKELLLTAPLVNQVILGIDPGFKTGCKLAVIDE
                     NGIFLDSAVIYPTKPKEDFINSSKIVLELIKKHKITAIAIGNGTASRETANFIDELLK
                     NEELRKENFEIKYAIVSEIGASVYSASKIASQEYPNLDVTIRGAISIAQRLRDPMAAL
                     VKIDPKSLGIGQYQHDVNQKELEKKLENITIDLVNKVGVDLNSASYKLLSFISGISEK
                     LAQNIVEHREKIKKFSSKNQLLEVKGLGEKAFIQSVGFLRIKDGLSILDNTAIHPEDY
                     ELTKKLQQNYKIEEIKDFEKTAIELNTTTLKLKDIINELLKPGYDVRTEFNQVKFSND
                     ILDINDLKEGFILNGIVRNITDFGAFVDIGLKNDALLHISQISQKRVSHPSEILSINQ
                     NLENLKVISVDLEKQRVGISLK"
     gene            complement(4277..4690)
                     /locus_tag="PKO10_00675"
     CDS             complement(4277..4690)
                     /locus_tag="PKO10_00675"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HNW65707.1"
                     /translation="MKNISSDIKGLKQYYKDTKNPICVISVEKTRSQKYLFEELKALF
                     SHIKILVIVDEKANDLENPYMLIWRVTNNMDSSRDLYIEGNTICLDGTNKNSLDGFTR
                     RWPGDVNCTKSVIDSLRERNIIDIDDEFIKKPEVY"
BASE COUNT         1900 a          624 c          691 g         1622 t
ORIGIN      
        1 gcatctacac tttttacaag ttctcttgca tcaaaaatct catttttatc aactccacca
       61 acaggacatg tcataatagg tctaggacca tcaccagatg ccattcttga agttaaattt
      121 acactattta gtaaatcaaa aatagctgga atatctttta tttcaatata gtgaaactga
      181 acattttgtc ttgtcgtaaa atcaaccaat ccttgagcat atttttgtcc aatctctccc
      241 ataactctta gttgttctaa attcattcta gcatcaacta atttgattct tttcataaaa
      301 tattttttat cttctaaacc atctttattt atgtgaggat acattccgta ccattttaaa
      361 agcccaatat actcatcttt caaaggaatt ccttctaatg cttctttgta taaatcgtct
      421 attactctaa gcggatttct tgaagctttt aaaagttcaa gttctgataa ttgtgccata
      481 ctattcctta ttataattgt aatttttgtg taattatatc tcgaaaagtt taaatacaaa
      541 ttaaaataat cttgattaaa taactatagt aaattataga taaatttaag ctatttttta
      601 gattaatatt taaaatatag tttataattc caccacagaa taaactttta ttctataaaa
      661 ctttaggaga atagtttgag tgcttcattt tcaaatgagc caaaagattt tactctattt
      721 ttagatttgc taaaacaact tattcgagtt ccttctgtta caggagctga acactctttt
      781 ttactttact taaaaagaga acttgaagag attggtataa aaacagagca ttatgatgga
      841 cttttagttg ctagtggaaa agatccatca aatggtatct taagtgcaca catagataga
      901 catggtgtta ttagtaccgg tccaaatgag ttccagtttg cagctttttt agcaaaaaat
      961 cgctctgatt taagaggaaa ttcactttca gaacaaactt ttcaacaaat ttcacaaaga
     1021 tatataaatc aaaatgttca tgcttacgag ccatttagtg gaggctattt aggtattgga
     1081 aaaattacag atgctttttt aaaagaagag ataaataatc taatatttaa aatagatggg
     1141 ttaaatcacc tacttcctgg tactccaatt gcctttgtag ataagcttaa acaaaatgga
     1201 aatttaatct ctgcacagct tgataatgtt ttaagtgcag caattatttt atatctttat
     1261 cagcatggat ttgctggaac tgcatttttt acagcccaag aagaagctgg taaaagttgg
     1321 aggtttgttc atgaatggtt taaaaaaaat aaaatatcaa cagataaact agttgttctt
     1381 gatactagtc cttatgaaag cagagaagaa gcagataagc aacttatagt tttaagaaat
     1441 aaggatgtaa atgcaagatt taaatcacct cttttaaagg agcttaaaca attttgtaaa
     1501 aaaaataaaa tatcattctc ttgtaaagac aaatttataa ttgaaaaaaa taaactaaga
     1561 gaaaaagatg ggttaaagcc tcttagtatt ggaagtacag agttaggaag aattgttatg
     1621 gagtcgaatg ggcaaattca aggcgcaact ttacaaattc caactactgg ttatcataca
     1681 gttgatgaga cagcttgtgt tgaatctatt aaagcaatta tttttatttt gagtagtttt
     1741 tatataaaaa ctgttaatat ttaattttaa aaaagaaggg aaaaatgaat ttagctatta
     1801 tttatgaatt aagagccgct gatgtaaaag aagaacatat tgagcaaatt atggataaag
     1861 taaaaaatag attaagtgaa gagaatatcg ataaagagct tgtaaagttg gggtatccta
     1921 aaatttttac tgttgattat agtgaatatg atagttttga ttataacgat agctttacac
     1981 caactagtaa agcctcttta aaagaagatt gaaaaactaa aaaaccttaa attttaaatt
     2041 cataagcttc ttgcttgtga atttagctac aatttccaaa aaaataagaa aagaaaattt
     2101 atgaatttaa tacaactact acaaaataaa acaaatctac ctataaatat tattgaaaat
     2161 ataataaaac ttcttgatga aggatgcact attcctttta ttgcaagata tagaaaagag
     2221 tttacaaatg gtgcaagcga tagtgagctt agagtatttg aagaagtttt tgaatattca
     2281 aaaaaattaa ttcaaagaaa agatgagata aaaaatctat taaaagagaa aaacttttta
     2341 gatgaaaaac taataaaatt tttagatgaa gctacctctt tacaagcttt ggaagatatt
     2401 tattcaccat ttaaagataa aaaatcttca agaactacaa ctgctttaga aaatggtctt
     2461 gaaccattgg caaatattat tcaaagtatg agatatacaa aagaggagtg tgaacaaaaa
     2521 gcaaatcaat ttttaaatga aaatataaaa actatcaatg aagcaataaa tggagcaaaa
     2581 gatataatag ctcaaagata tgcggatgat tttaaatcaa aagaagtttt aagaaattta
     2641 atacaaaatt ggggagtttt agaagtaact cctacaaaag agtttgataa aaatggtctt
     2701 tattcaaatt ttgcaaatat aaatgaaaaa ataaaatata taaaatctca tagagttttg
     2761 gcaattttaa gggctgtaaa tgaaaagcaa ctatcaatta aagttgaaat tgatgaaaat
     2821 catattttag aaaatattaa aaaatacaaa attccaacaa atgcttctag ctcaaaagag
     2881 ctagtatttg acggctttaa agatggttta aaaagacttc ttcttccaac tttaaaaaga
     2941 gaagctattt caaacttaaa agaaaaagct ggaagtgaag ctatagaact ttttggaaaa
     3001 aatctaaaag agttactcct tacagctcca cttgtaaatc aagtaatttt aggaattgac
     3061 cctggattta aaactggttg taagttagca gttattgatg aaaatgggat ttttttagat
     3121 agtgcagtta tatatcctac aaaacccaaa gaggatttca taaattcatc aaaaattgtg
     3181 ttagaactta ttaaaaaaca taagattact gctattgcta ttggaaatgg tacagcttca
     3241 agagaaactg caaattttat tgatgagtta ttaaaaaatg aagagttaag aaaagagaat
     3301 tttgaaataa aatatgcaat agttagtgaa attggagcta gtgtatattc ggcttcaaaa
     3361 atagcttcgc aagagtatcc aaatcttgat gtaacaataa gaggagctat ttctatagca
     3421 caaagactaa gagacccaat ggcagctttg gttaaaattg atccaaaatc attaggaatt
     3481 ggtcaatacc aacacgatgt aaatcaaaaa gagttagaga aaaaactaga aaatataact
     3541 atagatttag taaacaaagt tggagttgat ttaaactctg cttcatataa gcttctatct
     3601 tttatatctg gaattagtga gaaattagct caaaatatag tagaacatag agaaaaaatt
     3661 aaaaagttta gttcaaaaaa tcaactttta gaagtaaaag gtttgggaga aaaagcattt
     3721 attcaaagtg ttggattttt aagaataaaa gatggcttat ctattttaga caatactgca
     3781 attcatcctg aagattatga gctaacaaaa aaattacaac aaaactacaa aattgaagag
     3841 ataaaagatt ttgaaaaaac tgctattgaa ctaaatacaa ctactctaaa attaaaagat
     3901 attataaatg aacttttaaa accaggatat gatgtgagaa cagagtttaa tcaagtaaaa
     3961 ttttcaaatg atattttgga tataaatgac ttaaaagagg gatttatttt aaatggaatt
     4021 gttcgtaata taactgattt tggagcattt gtagatattg ggctaaaaaa tgatgcccta
     4081 cttcatattt cacaaattag tcaaaaaaga gtatctcatc caagcgaaat tttaagtata
     4141 aatcaaaatc ttgaaaatct aaaagtaata agtgtagatt tggaaaaaca aagagttggt
     4201 attagtttaa aatagatatt taattaattt tgataaaaaa agctatttta ctagctcttt
     4261 ttatcttttt ctgttcttaa tacacttcag gtttttttat aaactcatca tcaatatcaa
     4321 taatatttct ctctcttaaa ctatcaataa cactttttgt acaatttaca tctcctggcc
     4381 aacgtctagt aaatccatct aaagagtttt tgtttgttcc atcaagacag atagtatttc
     4441 cctctatata taaatctcta cttgaatcca tattatttgt aactctccaa attaacatat
     4501 atggattttc taaatcattt gctttttcat caacaattac taaaattttt atatgagaaa
     4561 atagagcttt taactcttca aaaagatatt tttggcttct tgttttttct acacttataa
     4621 cacaaattgg attttttgta tctttgtagt actgttttaa accttttata tcacttgaaa
     4681 tatttttcat tttacttaaa agctcactat cttctaaaag agtaattcct aactcatcta
     4741 tctcatcacc tttaaaatca agtccaagtt tgcaccaact gcaaatttag gagatgcatg
     4801 atcaagatga tctgtagcac ctcttgaaac taaaata
//
LOCUS       DAOQAN010000022         4815 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_22490_length_4815_cov_2.432143, whole genome shotgun sequence.
ACCESSION   DAOQAN010000022 DAOQAN010000000
VERSION     DAOQAN010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 4815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4815
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22490_length_4815_cov_2.432143"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="PKO10_00680"
     CDS             complement(<1..245)
                     /locus_tag="PKO10_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HNW65708.1"
                     /translation="MPNTNNQILKNTNSKLLDMFNASIMFDKKLYSQDIKGSIAHSKM
                     LALQNIITNDEQKSIEKGLLQVLEEIKSGEFEFSLEY"
     gene            322..1572
                     /locus_tag="PKO10_00685"
     CDS             322..1572
                     /locus_tag="PKO10_00685"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNW65709.1"
                     /translation="MNENLKEITKNTLEKLRQKNLPTTPENYFLEFKEQAKIFNTEYS
                     DVNILNEALKNLTNDEKKVLSSESIFEIIPILSKRANNDELKSLIGTFSDLLAPSVNF
                     ELKEEIEDFILDCLRNPIQITTKESILKIKNFAKNRIEADRRVLKEKTNDIIKLTSLM
                     SRYYDKTLSDSNSSNEDIKKIKEDLESLEISDFSKRELTAVQKRLINTIYKLEESLLE
                     NNRILSSNIDRFKQINKQIEQLERELQVTKEENLYDFLTSVLNRRAYEVEAKKTEKQF
                     FMFETNFAIVFFDIDHFKKINDKYGHACGDEILKSFAKILKELIRKEDIVARYGGEEF
                     VALINFRDKIEVIRYIKRVKNAFLNSTFIYKEHKINITFSAGVTFRNNYNSIVDAQTK
                     ADELLYEAKNRGRDKVIFDDGTIL"
     gene            complement(1590..2213)
                     /locus_tag="PKO10_00690"
     CDS             complement(1590..2213)
                     /locus_tag="PKO10_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNW65710.1"
                     /translation="MFAIYNNGTVGFRSTSDNLYALKNVEELESARFEPKEGLIQNFS
                     NELNKEKKDQFLNSYKKVATLDTLEPVYKARDIMTHDVIYARNDLTIVDIYYLIKDKK
                     VSQIPITGFGKKIIGIVNKKIILSLLMNHLDDTQAILKRKIDDVYLPEVLTADPDSDV
                     RDVVKVMLDLKLDAIPIVDDSDVLMGIISKTDILKAVANLPKLQLWS"
     gene            complement(2267..3199)
                     /locus_tag="PKO10_00695"
     CDS             complement(2267..3199)
                     /locus_tag="PKO10_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002850779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HNW65711.1"
                     /translation="MKNYSKKIEERINNPKNFGEITKEEARSLGCKLVVADFESNGND
                     TLRVYFAITKDKIVFNAKFKSFATGLIVALNDMMIELCIGKSIEKVANLFKTDVEFAL
                     RDEPQIPALSIEELHNSFLNFVILKKVALNSEKRDMNDFDDDYIVCDCARTNLKTIKD
                     AIKNFDLLTIEDIGNVTKAGIFCKSCQKEGGLEEKEIYLSDILEQTRAQIENEKQKDE
                     PSYINTSFEVMTKEQKIELIEDVLDDDVRPMLVMDGGNMEILDIVESPPHHDLYIRYL
                     GACSGCSSGSMGTLYAIESILQSKVNENIRVLPI"
     gene            3439..>4815
                     /gene="sufB"
                     /locus_tag="PKO10_00700"
     CDS             3439..>4815
                     /gene="sufB"
                     /locus_tag="PKO10_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HNW65712.1"
                     /translation="MSENQQIHDIINTDYKLGFETLVQSDTFAKGLNEDVIRAISAKK
                     EEPQFLLDFRLKAYEKWLKMEEPTWTNLEYPKIDYQDYAYYSAPKKALNSLDEVDPEI
                     LKTYEKLGIPLEEQKMLAGVAVDAVFDSVSIKTTYQDELEKLGIIFCSISEAAHRFPE
                     LVKEYLASVVPVTDNYFACLNSAVFTDGSFVYIPKNTRCPMELSTYFRINALNTGQFE
                     RTLIICDEGSYVSYNEGCSAPSRDDRQLHAAVVELVALKNSHIKYSTIQNWYPGDETG
                     KGGILNFVTKRALCKGDNSKVSWTQVETGSSITWKYPSCVLQGDNSVGEFYSVALTSR
                     AQQADTGTKMIHLGKNTKSTIISKGISAMKGINAYRGLVRVGKNAVNARNISECDSLL
                     IGHKCQAHTYPYHEIRNSSANIEHEATTSKISDEQLFYLNQRGIDEEDAIAMIVNGFC
                     KEVLKELPM"
BASE COUNT         1820 a          655 c          630 g         1710 t
ORIGIN      
        1 tcatactcta aagagaattc aaattcacca ctttttatct cttctaaaac ttgtaaaaga
       61 cctttttcga tacttttttg ctcatcattt gtaataatat tttgtaaagc caacatttta
      121 gagtgcgcaa ttgagccttt aatatcttga gaatatagtt ttttatcaaa cattatagaa
      181 gcattaaaca tatctaatag ttttgaattt gtatttttta aaatctgatt atttgtattt
      241 ggcataaaat tttcctttaa agattaagta aatattatat cataagtgat ataatatttt
      301 caaaaaactt aaggcacttt gatgaatgaa aatctaaaag agattactaa aaatacttta
      361 gagaaattaa gacaaaagaa cctaccaaca actcctgaaa actacttttt agagtttaaa
      421 gaacaagcaa aaattttcaa cactgaatat agtgatgtaa acatactaaa cgaagctcta
      481 aaaaatctaa caaatgatga aaaaaaagtt ctaagtagtg agtcaatttt tgaaataata
      541 cctattttat caaaaagagc aaataatgat gaattaaaat cactaatagg tacttttagt
      601 gatcttttag ctccttcagt aaattttgaa ttaaaagagg agattgaaga ttttattctt
      661 gattgtctta gaaatccaat acaaataaca acaaaagagt ctattttaaa aataaaaaat
      721 tttgcaaaaa ataggataga agccgataga agagttttaa aagagaaaac aaatgatatt
      781 ataaaactaa catctttaat gagtagatac tacgataaaa cactaagtga tagcaatagt
      841 tcaaatgaag atataaaaaa aataaaagaa gatttagaat ctttagaaat ttctgatttt
      901 tcaaaaagag agctaacagc tgttcaaaaa agattgatta atactattta taaacttgaa
      961 gaatctttac ttgaaaacaa tagaatttta tcatcaaata ttgatagatt taaacaaata
     1021 aacaaacaaa ttgaacagct tgaaagagaa cttcaagtta caaaagagga gaatttatat
     1081 gactttttaa cttctgtttt aaatagaaga gcctatgaag ttgaagcaaa aaaaacagaa
     1141 aaacagtttt ttatgtttga aacaaatttt gcgattgtat tttttgatat tgaccacttt
     1201 aaaaaaataa atgataaata tggtcatgct tgtggtgatg aaattttaaa atcttttgca
     1261 aaaatattaa aagaacttat aagaaaagag gatattgtag ctcgttatgg cggagaagag
     1321 tttgttgcat taataaactt tagggataaa atagaagtta taagatatat aaaaagagtt
     1381 aaaaatgctt ttttaaatag tacttttata tataaagagc ataaaataaa tattactttt
     1441 tcagctggag ttacttttag aaataattat aactctatag ttgatgctca aacaaaggct
     1501 gatgaactac tttatgaagc aaaaaataga ggtagagata aagttatttt tgatgatgga
     1561 acaattttat aaataaaaat ctaacttaac taagaccaaa gctgaagttt tggtaagttt
     1621 gcaacagctt ttaaaatatc tgtttttgat ataattccca ttaaaacatc actatcatca
     1681 actattggaa ttgcatctag ttttaaatca agcattactt taacaacatc tctaacatcc
     1741 gaatctggat cagctgttaa aacttctggc aaataaacat catcaatttt tctttttaaa
     1801 atagcttgtg tatcatctaa atgattcata agtaaagata aaattatctt cttatttact
     1861 ataccaataa tttttttacc aaaaccagta attggaattt gtgatacttt cttatctttt
     1921 attaaatagt agatatctac aattgttaag tcatttctag catatattac atcatgtgtc
     1981 ataatatctc ttgctttata aactggctcc agagtatcta aagtagcaac ttttttataa
     2041 gagtttagga attgatcttt tttctcttta tttaattcat ttgaaaaatt ttgaatcaat
     2101 ccctcttttg gttcaaacct agcagattct aactcttcca catttttcaa agcatataaa
     2161 ttgtcactag tacttctaaa tcctactgtt ccattgttat atatagcaaa catcaatatt
     2221 cctaaaaaag tttattttat tgtatcaaaa taactaatct tttatcttaa attggtaaaa
     2281 ctctaatatt ttcatttact ttactttgta aaatagactc aattgcatat aaagttccca
     2341 tgcttccaga actacaccca ctacaagcac ctaaatatct aatatataaa tcatgatgag
     2401 gaggtgactc tacaatatct aaaatctcca tatttccacc atccataaca agcattggtc
     2461 ttacatcatc atctaaaaca tcttctatca actctatttt ttgctctttt gtcataactt
     2521 caaaacttgt attaatatat gatggctcat ctttttgttt ttcattttct atttgcgctc
     2581 ttgtttgttc caaaatatca cttaaatata tctctttttc ttcaagtcca ccctcttttt
     2641 gacaagattt acaaaatatt ccagcttttg taacatttcc tatatcttca atagttaata
     2701 aatcaaaatt tttgatagca tcttttatag tttttaaatt tgttctagca caatcacaaa
     2761 ctatataatc atcatcaaaa tcgttcatat ctcttttttc agaatttaat gcaacttttt
     2821 ttagtatcac aaaatttaaa aaactattgt gtagctcttc aatactaaga gctggtattt
     2881 gtggctcatc tcttaaagca aattcaacat cagttttaaa caaatttgct actttttcaa
     2941 tactctttcc aatacataac tcaatcatca tatcatttag agcaactatt aaacctgttg
     3001 caaatgattt aaattttgca ttaaaaacta ttttatcttt tgtaattgca aaatatactc
     3061 ttaacgtatc atttccattt gattcaaagt ctgcaacaac tagtttacag cctaaacttc
     3121 gtgcctcttc ttttgttatc tctccaaaat tctttggatt gttaattctt tcctctattt
     3181 tttttgaata atttttcatt ttcattccat actttataat atttttttat ttttataata
     3241 atttttttgc attttattta aaataggata aattttatct taattttaaa tacgatttaa
     3301 taggcaataa attacttttt ttactaattc cttttacttt attagtattt atgtctttac
     3361 attatatact tattttgata ttattcaaat cggacaatat tagtcttaat taaaacaaat
     3421 aaattttatg gagaagtcat gagtgaaaat caacaaatac atgacattat aaacacagat
     3481 tataaattag gttttgaaac tttagttcaa agtgatacat ttgcaaaagg tttgaatgaa
     3541 gatgttataa gagcaattag tgcaaaaaaa gaagaacctc aatttttact agattttaga
     3601 ttaaaagcat atgaaaaatg gttaaagatg gaagaaccaa catggacaaa tttagaatat
     3661 ccaaaaatag attatcaaga ttatgcatac tattcagcac caaaaaaagc tttaaactct
     3721 ctggatgaag ttgatcctga gattttaaaa acttatgaaa agctaggaat tccacttgaa
     3781 gagcaaaaaa tgcttgcagg tgttgctgtt gatgctgttt ttgattcagt ttctattaaa
     3841 acaacttatc aagatgagct tgaaaaacta ggaattatat tttgctcaat tagtgaagca
     3901 gcacatagat ttccagaact tgtaaaagag tatttagcaa gcgttgttcc tgtaactgat
     3961 aactattttg cctgtttaaa tagtgctgtt tttacagatg gaagttttgt ttatattcca
     4021 aaaaatacaa gatgcccaat ggagttatca acatatttta gaataaatgc tttaaataca
     4081 ggacagtttg aaagaacttt aatcatttgt gatgaaggaa gctatgtttc atataatgaa
     4141 ggctgttctg ctcctagcag ggacgataga caacttcacg cagcagttgt tgaattagtt
     4201 gcacttaaaa atagtcatat aaaatattca actattcaaa actggtatcc aggtgatgaa
     4261 actggaaaag gtggaatctt aaattttgtt acaaaaagag ctttatgtaa aggtgataac
     4321 tcaaaagtat cttggacaca agtagaaaca ggttcaagta tcacttggaa atatccatca
     4381 tgtgttttac aaggtgataa tagtgtagga gagttttact ctgttgcatt aacatcaaga
     4441 gctcaacaag cagatactgg tacaaaaatg atacatttgg gtaaaaatac aaaatcaaca
     4501 attatatcaa aaggtatctc tgctatgaaa ggtataaatg cctacagagg attagttaga
     4561 gttggaaaga atgctgtaaa tgcaagaaat atttcggagt gtgactcttt attgattggt
     4621 cataaatgtc aagcgcatac gtatccatat catgagatta gaaatagtag tgcaaacata
     4681 gagcacgaag caacaacttc aaaaatttca gatgaacaac tattttatct aaatcaaaga
     4741 ggaatagatg aagaagatgc aattgctatg attgtaaatg gtttttgtaa agaggtttta
     4801 aaagagttac caatg
//
LOCUS       DAOQAN010000023         4728 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_23123_length_4728_cov_4.589129, whole genome shotgun sequence.
ACCESSION   DAOQAN010000023 DAOQAN010000000
VERSION     DAOQAN010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 4728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4728
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23123_length_4728_cov_4.589129"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="PKO10_00705"
     CDS             complement(<1..423)
                     /locus_tag="PKO10_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW65713.1"
                     /translation="MKTKPCLNCGKTIIKPQNESLKNWEYRHKFCSKSCSAIYRKVLV
                     KYQFKNGQKALHPIKKGQHLSKQTQFKKNQVPWNKNTKGLMNTWNKGKRFTKISGSLH
                     WNWKNGISKLNHLIRNLPEMKIWINYIFKRDNYTCKKCG"
     gene            complement(474..1481)
                     /locus_tag="PKO10_00710"
     CDS             complement(474..1481)
                     /locus_tag="PKO10_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW65714.1"
                     /translation="MELNIATLSDFVKNAQILFLKGLDSIKMETRNSGIFRVDNIPDY
                     SGNTKEYSEIDLEEYAKAKPEGDQTVRAKVQQGYSKIGTLKRIALDIGITYEMRHYNK
                     YQEVVSRLTNLGRVVGNRMELDLAHRITFATVTSYTDMDGATVDTSCGDTLQWAYTAH
                     TLKGTSATFRNRLANNPVFAKGSLEGMEQMIIENSVNQFGEKITIPYDILWTTDDPNT
                     VNTVREYLKSTASPDAINSGVVNVYSGKYRHVVLPRVATTATGAVDSTKAKYWGLASS
                     MYAQAVLAINEEPRLKVSSETGNSTDFSTEDYNFGAAAGYFIVTPGARGFTLSTGDAT
                     S"
     gene            complement(1514..1951)
                     /locus_tag="PKO10_00715"
     CDS             complement(1514..1951)
                     /locus_tag="PKO10_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW65715.1"
                     /translation="MAFKLYKGKTKTIWLNVKPSTAFAKDSIVAMGTSGDVGLIIPAT
                     SATAALSHIGVIPRAIASTDADYAATRKVPVLVPVEKNVEWLGDVTSGLVAADKGLLV
                     DLTDASTINRSASTIDAALVKSVISSTKGTFILNLQGSTGQAE"
     gene            complement(2161..2304)
                     /locus_tag="PKO10_00720"
     CDS             complement(2161..2304)
                     /locus_tag="PKO10_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW65716.1"
                     /translation="MEVHQAEFELLKRIRERYQYGEITIVIKDGLPTDIVREYHKDKI
                     WFG"
     gene            complement(2327..2689)
                     /locus_tag="PKO10_00725"
     CDS             complement(2327..2689)
                     /locus_tag="PKO10_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW65717.1"
                     /translation="MEEKTPMGEIFVEVSFEDGSKEVMPKKRLEMISTEAISSDSLVR
                     EVLRKQVGSILFGVMHEYGIKIDEVDPCIDTVVELVNNSSAKSYEILFGYEKTKIPLN
                     AINKILMANLKDNGDKTS"
     gene            complement(2736..3323)
                     /locus_tag="PKO10_00730"
     CDS             complement(2736..3323)
                     /locus_tag="PKO10_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW65718.1"
                     /translation="MAKKDIIKEKGASTGGEEIINTANTDSSVTGENSPAGNAPEKKE
                     EMVQVRREDLNEILGRLEKQSKEIDLLMKASDKERMARAQSKDGKILVHSVKIAKWDD
                     TDNYVIGWKAATNKAEVINGRYIEDLTTNVFFEEAESIVVPMLEFYRKTIRKDSADVI
                     GKEVKVDDKGNKVELLKIRFENGKELVINSNFVNL"
     gene            complement(3327..3947)
                     /locus_tag="PKO10_00735"
     CDS             complement(3327..3947)
                     /locus_tag="PKO10_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW65719.1"
                     /translation="MSKIKIINDKGEEEEIDAFTEAEVTAKVKEKEEEFTKVIGEKDE
                     TLKNLTKEKDELVKKAGDIKQDNPNFAILKSALDKKDKEIEDIKISLEKDKEERKISG
                     LITKFTRGNEELEKKIRFHLENSVAGMKSDTPENLTKKIDAAIKLSQDFGSEGIFDGG
                     VGGGGKGGDIKGGDNGGVEFTAKEKALGAKLGISEEDYKKYGPKLK"
     gene            complement(3940..4572)
                     /locus_tag="PKO10_00740"
     CDS             complement(3940..4572)
                     /locus_tag="PKO10_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUMOD3 domain-containing DNA-binding protein"
                     /protein_id="HNW65720.1"
                     /translation="MPKGVYKRTEETKQKLRLCNLGKKHTEEWKKNMSNFMLGNKYSL
                     GFKVKKETKLKISQKLKGKKKSKQHCINIGKAKIGNKNMLGKFHSKKTKQKLSKFHSK
                     LIGELNPRYIKDRTKLKKRRDGFHGNDSACNNFRQKVKERDKKRCLMRNKECNGQLEV
                     HHILSWRKYPKLRYDINNGITLCHFHHPRGRVVEEKMVEYLKELINKNNE"
BASE COUNT         1341 a          915 c          566 g         1906 t
ORIGIN      
        1 accacatttt ttacaagtat aattatctct tttaaaaata taatttatcc atattttcat
       61 ttctggtaaa tttcttatta aatgatttaa tttacttata ccatttttcc aattccaatg
      121 taatgaacct gaaatttttg taaatctttt acctttattc caagtattca ttaaaccttt
      181 tgtattctta ttccaaggaa cttgattctt tttaaattga gtttgtttag ataaatgttg
      241 tccttttttt attggatgta acgctttttg tccattttta aattgatatt taacaaggac
      301 ttttctataa attgcactac aacttttaga acaaaattta tgtctatatt cccaattttt
      361 taaagattca ttttgaggtt ttattattgt ctttccacaa ttaagacatg gttttgtttt
      421 catataattt aagtataaac caaatactta aattatgcaa tagcatcacc tgtttacgaa
      481 gttgcatctc ctgtggacag tgtaaaccct ctagcacctg gtgtaacaat gaagtaacca
      541 gctgctgcac caaagttgta atcttcagtt gagaagtctg tgctatttcc tgtttcactt
      601 gataccttca aacgaggttc ttcgttgata gctaatactg cttgagcata cattgaagat
      661 gctagacccc agtattttgc ttttgtagaa tcaactgcac ctgtagctgt tgtagctaca
      721 cgaggaagca caacatgacg atatttacca gagtaaacat tgactactcc agagttaata
      781 gcatctggtg atgctgttga ctttaagtat tcacgaactg tgtttactgt gtttggatca
      841 tcagttgtcc aaagaatgtc ataaggaata gtgattttct caccaaattg gttgacacta
      901 ttctctataa tcatttgttc cataccttct aatgaaccct ttgcaaatac agggttatta
      961 gctaaacgat ttctgaaagt tgcacttgtt cccttcaaag tgtgagctgt gtaagcccat
     1021 tgtagggtat ctccacaaga tgtatcaact gttgcaccat ccatatctgt ataagatgtg
     1081 actgttgcga aagtgattct gtgagcaagg tcaagttcca tacgatttcc aacaacacga
     1141 ccaagatttg ttaaacgaga aacaacttcc tggtatttat tgtagtgtcg catttcgtaa
     1201 gtaataccaa tgtcgagagc aattctcttc aatgttccta tcttactgta accttgctga
     1261 acctttgcac gaactgtttg gtcgccctct ggtttagctt ttgcatattc ctcaagatca
     1321 atttcggaat attcttttgt gtttccagaa taatctggaa tattatctac tctgaaaatc
     1381 ccagaatttc ttgtttccat cttgatacta tcaagacctt ttaagaaaag gatttgagca
     1441 tttttcacga agtcagataa tgttgctata ttaagttcca tatatttttt ataaaattaa
     1501 ttgataatga taattactct gcttgaccag ttgaaccttg aagattcaag atgaatgttc
     1561 cttttgttga agagataaca ctcttaacta aagcagcatc aatagttgaa gcacttctgt
     1621 taattgttga agcatctgtc aaatcgacca ataaaccttt atctgctgct acaagaccac
     1681 ttgtaacatc tcctaaccac tctacatttt tttctactgg gactagaaca ggaacttttc
     1741 ttgttgctgc atagtctgca tcagttgatg caattgctct tggtataact ccaatgtgag
     1801 atagagctgc agttgctgaa gttgcaggaa taattaatcc tacatcacca cttgttccca
     1861 tagctacaat ggaatctttt gcaaaggcag ttgatggctt tacattaagc caaattgtct
     1921 ttgtcttacc tttatataat ttgaatgcca tatatatttt aaagtttaag tttataataa
     1981 atttttttgt ttggacactc tatgttttaa acaacttttc atgttgtgat gtcttccgac
     2041 agaatgcttt cctcccaaca aaaaaacaga ggaaaaagaa taatcttttt gtccctctgt
     2101 ttttcagcta gacatattta tattataatt ataatcccta tataaataca agtcaagtaa
     2161 ttatccaaac caaatcttat ctttatgata ttctctgact atatctgttg gaagtccatc
     2221 ttttataact atggtaatct caccatactg gtatctctct cgaattctct tcaaaagttc
     2281 gaattcagct tggtgaactt ccatttgaaa cttcctatct tcttttttaa gaggttttat
     2341 ctccattgtc ttttaaatta gccattaata tcttattaat tgcatttaat ggaatctttg
     2401 tcttctcata accaaaaaga atctcataac ttttagctga agagttatta acaagctcta
     2461 caactgtatc aatacatgga tcaacttcat caattttaat tccatactcg tgcataacac
     2521 caaaaagtat tgaaccaacc tgtttcctca atacttctct tactagacta tcactagaaa
     2581 ttgcttcagt agaaatcatt tctaatctct tttttggcat tacttcttta ctcccatctt
     2641 caaatgaaac ttctacaaat atttctccca ttggtgtctt ctcttccatt tctgtgacca
     2701 ttaatatttt tttatcagct atgtattttt ccatattata aatttacaaa attactatta
     2761 ataactaatt ccttaccatt ctcaaatcga attttaagaa gttcaacctt atttcccttg
     2821 tcatcaactt ttacttcctt accaataaca tctgctgaat ccttacgaat tgttttgcga
     2881 taaaattcta gcataggaac aacaattgat tctgcttctt caaagaaaac atttgttgtt
     2941 aaatcttcaa tatatcttcc attaataact tctgctttat ttgttgcagc tttccaacca
     3001 ataacatagt tatcagtatc atcccacttt gcaattttaa cactatgaac aagaatcttc
     3061 ccatccttac tttgagctcg tgccattctt tccttgtcag aagctttcat aagaaggtca
     3121 atttctttcg actgtttttc taatcttcca agaatttcat tcaagtcttc tcttcgaact
     3181 tgaaccattt cttctttttt ttctggagca tttccagctg gtgaattttc accagttaca
     3241 gaactatcag tatttgcagt atttattatt tcttcaccac ctgttgaagc acctttttct
     3301 tttattatat cttttttagc catatattat tttaattttg gaccatattt cttataatct
     3361 tcttcactaa ttccaagctt tgcaccgaga gctttttctt tagctgtgaa ttcgacccca
     3421 ccattatcac ctcctttaat atcaccaccc ttaccacctc caccaacacc accatcaaat
     3481 attccctctg aaccaaaatc ttgtgacaac tttatcgcag catcaatttt ctttgttaaa
     3541 ttttctggtg tatctgactt cattccggca acactatttt ctaaatgaaa tcttattttc
     3601 ttttctaact cttcattacc tcttgtgaac ttggtaatta aaccactaat ttttctttct
     3661 tctttgtctt tttcaagact tattttaatg tcttcaattt ccttgtcctt tttatctaga
     3721 gcacttttta atatggcgaa attaggatta tcttgcttaa tatctccagc tttttttact
     3781 aattcatctt tttcttttgt aagattttta agtgtttcgt ctttttcacc aataaccttt
     3841 gtgaactctt cttctttttc ttttaccttt gctgtaactt cagcttcagt aaaagcatca
     3901 atttcttctt cctcgccctt atcatttatg atttttattt tactcattgt ttttattaat
     3961 taattctttt aaatattcaa ccattttttc ttctaccact cgaccccttg gatgatgaaa
     4021 atgacataaa gtaattccat tattaatatc gtatcttaat ttaggatatt ttctccaaga
     4081 taaaatatgg tgcacttcta actgaccatt acattcttta ttcctcatca aacatctttt
     4141 tttatccctt tccttaactt tttgtctaaa attattacaa gccgaatcat ttccatgaaa
     4201 accatctctt ctttttttaa gttttgttct atctttaata tatctagggt ttaattcgcc
     4261 aattagtttt gaatgaaatt tacttaattt ttgtttagtt tttttagaat gaaattttcc
     4321 aagcatattt ttattcccaa tttttgcttt cccaatatta atacaatgtt gtttagattt
     4381 tttctttcct ttcaattttt ggctaatctt taatttagtt tcttttttta ctttaaatcc
     4441 taatgaatat ttattaccaa gcataaaatt actcatattt tttttccatt cttcagtgtg
     4501 ttttttacct aaattacata atcgtaattt ttgtttagtt tcttctgttc ttttataaac
     4561 tcctttaggc atatatttta gttttctcca tatatagttt taaatttatc tataaactgt
     4621 ttatcgtcag aaaatttact acgaagttcc tcaagattcc ccaaatgttc atttgtcctc
     4681 gtcagcatcc attcattgat taaccggaat acctcaatac ttgctctg
//
LOCUS       DAOQAN010000024         4684 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_23410_length_4684_cov_2.730611, whole genome shotgun sequence.
ACCESSION   DAOQAN010000024 DAOQAN010000000
VERSION     DAOQAN010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 4684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4684
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23410_length_4684_cov_2.730611"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..795)
                     /locus_tag="PKO10_00745"
     CDS             complement(<1..795)
                     /locus_tag="PKO10_00745"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HNW65721.1"
                     /translation="MELWIKKIESASTLEELESLRVDTLGKKGVLTIEFAKMKDIPAD
                     DKKSFAENLNRQKDEITRALDSKKIELEAIALDKKLQSEKIDVTKFNNELSCGAAHPV
                     AETMNRIINYFLNLNFTVEEGPLVEDDFHNFQALNLPEYHPARDMADTFYNKDYTLLR
                     THTSPVQIRTMLSQKAPIRMIAPGTVFRRDFDITHTPMFHQIEALVVDEADKISFANL
                     KHVLVEFLQHMFGEVEVRFRPSYFPFTEPSAEVDISCVFCKGSGCRV"
     gene            922..1266
                     /locus_tag="PKO10_00750"
     CDS             922..1266
                     /locus_tag="PKO10_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HNW65722.1"
                     /translation="MCIFCKIVNKEIPSNIVLEDDNFLAFHDINPTRKIHVLVIPKSH
                     YDSFEDTPSDIMSDLSSFIKKVTKELKIDKSGYRMITNIGNDGGQEVPHLHFHLIGGE
                     KVGRLVRDKNDL"
     gene            complement(1294..2229)
                     /gene="accA"
                     /locus_tag="PKO10_00755"
     CDS             complement(1294..2229)
                     /gene="accA"
                     /locus_tag="PKO10_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HNW65723.1"
                     /translation="MATYLEFEEKIKKIEEDIIIAKTKSDEAAVEILNKKLEKEVEKT
                     FKNLNDYQKLQLARHPDRPYALDYINAILSNAYEIHGDRHFVDDNAIVCYLGFIGTQK
                     TLVIGEQKGRGTKEKLKRNFGMPSPEGYRKALRAAQIAEKFQIPILMLVDTPGAYPGL
                     GAEERNQSEAIAKNLYEFANFTTPTVSVVIGEGGSGGALAISVADKLAMMRYSVYAVI
                     SPEGCSAILWNDPSKVETAAQALKITAENLKQQNLIDDVINEPLIGAHRNKDEAYMAL
                     KEYFLNSVEELKKLTPQERQEKKYQKIMNLGSFSE"
     gene            complement(2277..3536)
                     /locus_tag="PKO10_00760"
     CDS             complement(2277..3536)
                     /locus_tag="PKO10_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HNW65724.1"
                     /translation="MKRVVITGLGTINSTGHSVNESFDAVVNGVCGIDKITLFDTTDF
                     SVQIAGEVKNFDPETVMDKKEVKKADRFIQLGIKAALEAMIDSGFVTSENKKVDNSIA
                     NRFGIISGSGIGGLSTIQKNSTICDTKGSRKISPFFIPSSLANMLSGFISIEHNLKGP
                     SLSHVTACAASTHALNDAMKTIVLGGADRILVVGAESAICGAGVGGFAAMKALSTRND
                     DPKKSSRPFDIDRDGFVMGEGAGALVLETLDSAIARKAKIYCEIIGFGESGDANHITS
                     PVIDGPLRAMRAAFEMAKELTGEYPKIDYINTHGTSTPVGDKNETAAIKELFNGKDNC
                     PPVSSTKGQIGHCLGAAGAIEAIMAIKALNEGVIPPTINVENQDPECDLDVVPNVARK
                     VDLTTVMSNNFGFGGTNGSVIFKKFTK"
     gene            complement(3646..3876)
                     /gene="acpP"
                     /locus_tag="PKO10_00765"
     CDS             complement(3646..3876)
                     /gene="acpP"
                     /locus_tag="PKO10_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012857599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNW65725.1"
                     /translation="MALLDDVKAVVVDQLDCDPAEVKEDSKFIEDLGADSLDVVELVM
                     ALEEKFDIEIPDEEAEKILTVGDAISYIENNK"
     gene            complement(3968..>4684)
                     /gene="fabG"
                     /locus_tag="PKO10_00770"
     CDS             complement(3968..>4684)
                     /gene="fabG"
                     /locus_tag="PKO10_00770"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002790539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HNW65726.1"
                     /translation="ITGASKGIGAEIAKNLASYGLKVWINYRSKIEEAEAIKEQIEKA
                     GGTAAIIKADVSIEEQFVAAIKTIIESDGELAYLVNNAGITKDKLALKMSVEDFTSVI
                     DANLTSAFIGCREFFKTRGKKGFGAVVNISSIVGEMGNPGQANYSASKGGLNAMTKSF
                     AKEAASRNIRYNAITPGFIQTDMTDLLKAEVKADYEKNIPLSRFGNPSEIADAVAFLL
                     SDHSSYITGEILKVNGGLYV"
BASE COUNT         1514 a          836 c          587 g         1747 t
ORIGIN      
        1 tacacggcaa ccacttcctt tgcaaaaaac acatgaaata tcaacttcag cagaaggttc
       61 tgtaaatggg aaatatgaag gtctaaatct aacttcaact tctccaaaca tgtgttgtaa
      121 aaattcaact aaaacatgtt ttaaatttgc aaatgagatt ttatctgctt catctacaac
      181 taaagcttca atttgatgaa acattggagt gtgagtgata tcaaaatctc ttctaaaaac
      241 agttcctgga gcaatcattc ttataggagc tttttgactt agcattgttc ttatttgaac
      301 aggagaagta tgtgttctta aaagtgtata atctttgttg taaaaagtat ctgccatatc
      361 tcgcgctgga tgatattcag gtaggtttag tgcttgaaaa ttatgaaaat catcttctac
      421 tagtggacct tcttcaaccg taaaattaag gtttaaaaag tagttaataa ttctattcat
      481 agtctctgca actgggtgag cagcaccaca actaagttca ttattaaact ttgtaacatc
      541 aattttttca gattgtaatt ttttatctaa agcgattgct tctagttcta tttttttgct
      601 atctaaagct cttgttattt catctttttg tctatttaga ttttctgcaa atgatttttt
      661 atcatcagct ggaatatcct tcatttttgc aaattctata gtcaaaactc ccttttttcc
      721 aagggtatca actcttaaac tctccagttc ttcaagtgtt gaagcagatt cgattttctt
      781 tatccaaagt tccaattttt attcctaatc tctaatttat tttttacaaa atatttattt
      841 ataatctctc aaagttgcga ttatacctaa ttttttatta gagtttggtt ataatttatt
      901 ataaaaaatc aaggagaaaa tatgtgcatt ttttgcaaaa ttgtaaataa agagataccc
      961 agcaatattg tattagaaga tgataatttt ttagcttttc atgatataaa tcctacaaga
     1021 aagattcatg ttttagtgat tccaaagtct cattatgatt catttgaaga tactccatct
     1081 gatattatgt ctgatttaag tagttttata aaaaaagtta caaaagagtt aaaaatagat
     1141 aaaagtggat atagaatgat tacaaatatt ggaaatgatg gaggacaaga ggttccacac
     1201 ttacattttc atttaattgg tggtgaaaaa gttggaagat tagttagaga taaaaatgat
     1261 ttataagata aactaaaaag tttatcttat ttattactca gagaaagatc ccaaattcat
     1321 aattttttga tattttttct cttgtctctc ttgtggagtt agttttttaa gctcttcaac
     1381 cgagtttaaa aaatactctt ttaaagccat ataagcttca tctttatttc tatgagcacc
     1441 aattagaggc tcatttataa catcatctat tagattttgt tgctttagat tttcagctgt
     1501 aattttaagt gcttgtgctg ctgtttcaac ttttgatggg tcattccata aaatagcact
     1561 acaaccctca ggtgatataa cagcatatac agaatatctc atcatagcaa gcttatcagc
     1621 aacagatata gctaaagctc caccactacc accttctcca attacaactg aaactgttgg
     1681 agttgtaaaa tttgcaaact cataaagatt ttttgcaatt gcttcacttt gatttctctc
     1741 ttcagctcca agccctggat atgcacctgg agtatcaact aacataagaa tcggaatttg
     1801 aaatttttct gctatttgtg cagctcttaa agcttttcta taaccttcag gactaggcat
     1861 tccaaagttt ctttttagtt tctcttttgt tccccgacct ttttgttcac caattactaa
     1921 agttttttga gttccaataa aacctaaata acaaacaatt gcattatcat ctacaaagtg
     1981 tctatctcca tgaatttcgt aagcatttga taaaatagca tttatataat caagtgcata
     2041 aggtctgtct ggatgacgtg caagttgaag tttttgataa tcatttaaat ttttaaaagt
     2101 tttttcaacc tctttttcaa gttttttatt taatatttca acagcagctt catccgattt
     2161 tgtttttgca attattatat cttcttcaat tttctttatt ttttcttcaa attctaagta
     2221 agtagccaat atttttcctt aataaaaagg gtagcaatac cctttaaggt ttagatttat
     2281 tttgtaaatt ttttaaaaat tactgaaccg tttgttccac caaaaccaaa gttatttgac
     2341 ataacagttg ttaaatcaac ttttctagca acatttggaa caacatccaa atcacattct
     2401 ggatcttggt tttcaacatt tattgtagga ggaataacac cttcatttaa agctttaata
     2461 gccataatag cttcaatagc tccagcagca cctaaacagt gaccaatttg cccctttgta
     2521 gatgatacag gagggcagtt atcttttcca ttaaaaagtt ctttaatagc agctgtctca
     2581 tttttatctc caactggagt tgaagttcca tgagtattta tatagtcaat ttttggatac
     2641 tctccagtta actcttttgc catttcaaat gcagctctca tagctcttaa aggaccatct
     2701 attacaggtg aagtgatatg atttgcatca ccactctctc caaaaccaat aatctcacaa
     2761 tatatttttg cttttctagc tattgcacta tcaagagttt caagaactaa agcaccagct
     2821 ccttcaccca ttacaaatcc atctctatct atatcaaaag gtcttgaaga ttttttaggg
     2881 tcatcatttc tagtagataa tgctttcata gcagcaaatc caccaacacc agctccacaa
     2941 atagcacttt cagccccaac aactaaaatt ctatcagctc cacctaaaac aattgttttc
     3001 atagcatcat ttaatgcatg agttgaagca gcacaagcag taacatggct taaagatgga
     3061 ccttttaaat tatgttcaat agagataaac ccacttagca tatttgctaa agatgatggt
     3121 ataaaaaatg gagatatctt tcttgaacct tttgtgtcac aaattgtaga atttttttga
     3181 attgtactta atccaccaat tccagaacct gaaattattc caaatctatt tgctattgaa
     3241 ttatctactt ttttattttc acttgtaaca aaaccactat caatcatagc ttctaaagct
     3301 gcttttatac ctaattgaat aaatctatca gcttttttaa cctctttttt atccattaca
     3361 gtttcaggat caaaattttt tacttcacct gcaatttgaa ctgaaaaatc agttgtatca
     3421 aacagtgtaa ttttatcaat tccgcaaaca ccgtttacaa cggcatcaaa agattcgttt
     3481 acactatgtc cagtagaatt tatagtacct aatcctgtaa taacaactct tttcatctaa
     3541 taatgctcca atattttatt taaactattc aatttttaaa ttaaaaaact caataataaa
     3601 aacaatagat aaaagagtaa ctcttttatc taaaaaaaga aaaaactatt tgttgttttc
     3661 aatgtatgat attgcatcac caacagttaa tattttttca gcttcttcat cagggatttc
     3721 gatatcaaat ttctcttcta aagccattac aagctccaca acatctagtg aatctgctcc
     3781 taagtcttca atgaactttg aatcttcttt tacttcagct ggatcgcaat ctaattgatc
     3841 aacaacaaca gcttttacat catctaataa tgccatttta attcctttaa taaaattgtc
     3901 ccaaaattat agcaaaaaag ttttaaaaac tttttaattt aaataaatta tagtttaaag
     3961 ataaacttta aacgtataat ccaccattta cttttagaat ttcacctgtg atatatgatg
     4021 agtgatcgct tagtaaaaat gctactgcat ctgcaatttc acttggattt ccaaatcttg
     4081 aaagagggat atttttttca taatcagctt ttacttcagc ttttaataaa tctgtcatat
     4141 ctgtttgaat aaatcctgga gttattgcat tgtatctaat atttctacta gccgcttctt
     4201 ttgcaaatga ttttgtcata gcatttaatc caccttttga agctgaatag tttgcttgac
     4261 caggatttcc catctctcct acaattgaag atatatttac aacagctcca aaaccttttt
     4321 tacctcttgt tttaaaaaac tctctacaac ctataaatgc tgaagtaaga tttgcatcaa
     4381 ttacagatgt aaaatcttca acactcattt taagagctag tttatctttt gttattccag
     4441 cattatttac caaatatgcc aactctccat cactttcaat aattgtttta atagcagcta
     4501 cgaactgttc ttctattgat acatcagctt ttataattgc tgctgttcct ccagcttttt
     4561 caatttgttc ttttatagct tctgcttctt caatcttact tctataattt atccaaactt
     4621 ttaaaccata gcttgctaaa ttctttgcaa tttcagcacc aatacctttg cttgcacctg
     4681 ttat
//
LOCUS       DAOQAN010000025         4665 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_23535_length_4665_cov_3.095228, whole genome shotgun sequence.
ACCESSION   DAOQAN010000025 DAOQAN010000000
VERSION     DAOQAN010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 4665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4665
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23535_length_4665_cov_3.095228"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..177)
                     /locus_tag="PKO10_00775"
     CDS             complement(<1..177)
                     /locus_tag="PKO10_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002861092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HNW65727.1"
                     /translation="MLEGIVRDSITKPAVKALRKDGYLIANIYGKGMENISAAFKRNE
                     YIKYLKNKTTLAFDI"
     gene            363..1223
                     /gene="folD"
                     /locus_tag="PKO10_00780"
     CDS             363..1223
                     /gene="folD"
                     /locus_tag="PKO10_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014473493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HNW65728.1"
                     /translation="MVLLDGKILSAKIKEEVKVEVTQIVKEKNITPGLAVILVGNDAA
                     SATYVASKAKACKDAGIYSVVHEMPESITQEELLETINMMNNNPKLDGILVQLPLPKH
                     IDTTTVLEAINPLKDVDGFHPYNVGRMVSNLDSFLPATPFGVMRMFEEHNIELSGKDV
                     VVIGSSDIVGKPMASLLINKKATVTVCNSRTKDLKAHTSKADIVIIAVGVPYLLKEDM
                     VKDGAVVIDVGINRLESGKLVGDADFEGLKNKCSFLTPVPGGVGPMTIAMLLKNTIKA
                     SKLRDKREIK"
     gene            1224..2005
                     /gene="lepB"
                     /locus_tag="PKO10_00785"
                     /pseudo
     CDS             1224..2005
                     /gene="lepB"
                     /locus_tag="PKO10_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510692.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
     gene            2005..2460
                     /gene="rpiB"
                     /locus_tag="PKO10_00790"
     CDS             2005..2460
                     /gene="rpiB"
                     /locus_tag="PKO10_00790"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HNW65729.1"
                     /translation="MKYFIGADHAGIEIKAFVKDLFEQRGHEVEDLGPYNKDRVDYPD
                     FAAKVCKKVLENSGSMGILICGSGIGMSMAANKFDGIRAALCHNEYSARMARKHNDAN
                     VLCLGERVSGEGMIEAIIKAWDKAEFEEGRHTQRVEKINALSKIGNCRA"
     gene            2488..2619
                     /locus_tag="PKO10_00795"
     CDS             2488..2619
                     /locus_tag="PKO10_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW65730.1"
                     /translation="MADWQVYLMMTAIVAAMLIGKKLMFFDSNKKKSDDKKINKDEN"
     gene            2616..3845
                     /locus_tag="PKO10_00800"
     CDS             2616..3845
                     /locus_tag="PKO10_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNW65731.1"
                     /translation="MINPKIIEYIFSSASIQRWNDYPRMVDLVELDKQAHKFIIAYFI
                     AKLEDDINFTHLIEAGIFEFLRRVVVTDIRPDVFRKALQQKSKEINTWVVGKLKSSIE
                     NIENGNFLQKFEEFLNDPNMYKKERFILKAASYLSTKWEFSIVYQTSQFLSDIEEVKK
                     AVDAEIEDYYELIGVRKIALNKKLAKIVDLSGRLRFQKRWAQTPRIPETSVLGHMLTV
                     ALFSYFYSLEVKACDKRLQNNFFTALFHDLPEALTRDIISPVKYSVDELSEIIAEYEV
                     AKIEDDILPNIPINIQEEFSYILGISKGIKEEFANKVKIDGNITEVDDINKYNLDKYE
                     AIDGLALKQCDKLSAFVEASLSISHGIKSKELISGKKEILKGLKEINGVDFKQIALDI
                     DSEFGSNGQIQTTLDFE"
     gene            3874..>4665
                     /locus_tag="PKO10_00805"
     CDS             3874..>4665
                     /locus_tag="PKO10_00805"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNW65732.1"
                     /translation="MLLTAEQEEIVNSSLDSFKINAVAGSGKTTTLLEYAKKNSNLKI
                     LYLAYNKSLQIALNEKLKDYHLPNLHISTIHSLAYNKTEAYKYKLTPELKTNILEKLI
                     INHEFQDNKKSYYPSLEYTTILKNFINFYCNSNLIELDLKLLEEFKKQNDFGVKILDI
                     LNKKEKKLLEHLKLTLSSMKLGKIDAIHDFYLKMFYLNKKISSNLNYDLILIDEAQDI
                     SDVMIGIIENQKCRKIYVGDSFQQIYSFRYASNALNKIDLPSYELT"
BASE COUNT         1854 a          520 c          783 g         1508 t
ORIGIN      
        1 tatatcaaaa gcaagagtag ttttattttt taaatatttg atatactcat ttcttttaaa
       61 tgctgcagat atgttctcca taccttttcc atagatattt gcaattaaat atccatcttt
      121 tcttaaagct tttacagctg gtttagttat actatctctt acgataccct ctaacatttt
      181 agtcctttgt tttaatttta aattgaacgc ggattatatt taaaatttac taaatattag
      241 gttaaattat aagttatatt tgtaagcaac aagatatctc aatatgctaa aaaatatttt
      301 aattcaattt tagatacaat caaatctaaa aaatttaaat aacttaatta taaggtaaaa
      361 aaatggtact actagatggg aaaattttat cagcaaagat aaaagaagag gtaaaagttg
      421 aagttactca aattgtaaaa gagaaaaata taactccagg acttgcagtt attcttgtag
      481 gaaatgatgc agcaagtgca acttatgttg caagtaaagc aaaggcttgt aaagatgctg
      541 gaatttattc agttgtgcat gaaatgccag agagtataac tcaagaagag ttacttgaaa
      601 ctataaatat gatgaacaat aatccaaaac ttgatggtat tttagtacaa cttccacttc
      661 caaaacatat agatacgaca actgttttag aagctataaa tccattaaaa gatgttgatg
      721 gtttccatcc atacaatgtt ggaagaatgg tttctaacct tgattctttc ttaccagcaa
      781 ctccatttgg tgttatgaga atgtttgaag agcataatat tgaattaagt ggaaaagatg
      841 ttgtagttat tggaagtagc gatattgttg gaaaacctat ggcatcactt ttaataaata
      901 aaaaagcaac agttacagtt tgtaatagta gaacaaaaga tttaaaagct cacacttcaa
      961 aagctgatat tgtaattatt gctgttggtg ttccatatct actaaaagaa gatatggtta
     1021 aagatggtgc tgttgttatt gatgtaggaa ttaatagact tgagagcggt aaacttgttg
     1081 gtgatgctga ttttgaagga cttaaaaaca aatgttcttt tttaactcca gttccaggtg
     1141 gagtaggacc aatgacaatt gcaatgcttt taaaaaatac aataaaagct tctaaactaa
     1201 gagataaaag agaaataaaa tagatgttaa aaaagattta taactggtca tcatcttgga
     1261 ctggtacaat agtaattgta cttacaatta tattttttgt tgctcaagcc tttgtaattc
     1321 ctagcggaag tatgaaaaat acacttttaa taggtgatat gctttttgtt aaaaaattcg
     1381 catatggagt tccaactcct acaattcctt ggattgaagt aaaagtttta ccagatttta
     1441 aggaaatgga catttaatag aaggtgagag accaaaaaga ggtgatatcg tagtatttag
     1501 atatccacat gatccagata ttcactatgt aaaaagagca gtagctacaa gtggagacac
     1561 tatattttta aaaaataaaa ttttatatct tcaccctaaa gagggaaatg attttgtaaa
     1621 aactgaattt aaagattatg aaactattga tatagatgga aaactttttg ttgtaagtcc
     1681 atacgaaaaa gagcataaag gaattcataa cgatcctaat gtcactagaa atctaatagc
     1741 tccaaaagaa ctatttgata tgcaagagat tgttatacca gaagatgaaa catttatgat
     1801 gggagacaat cgtgaccact caaatgattc aaggttttgg ggaagtgtac cttataaata
     1861 tatagttgga aaaccttggt ttatctattt ttcttgggat gatgattaca aaattagatg
     1921 ggatagagtt tttagaactg ttgaaagttt agaaaaagat attgatacaa cagctgaaat
     1981 taagcatgag gaaggaattt attaatgaaa tattttatcg gtgcagatca tgctggaatt
     2041 gaaataaaag catttgtaaa agatttgttt gagcaaagag gacatgaagt tgaagattta
     2101 ggtccatata ataaagatag agttgattat cctgattttg cagcaaaagt ttgcaaaaaa
     2161 gttttagaaa atagtggttc aatgggtatt ttaatttgtg gaagtggaat tggaatgagt
     2221 atggctgcaa ataaatttga tggaataaga gcagctcttt gtcacaatga atacagtgca
     2281 agaatggcta gaaaacacaa tgatgcaaat gtactttgtt taggtgaaag agtaagcggt
     2341 gaaggtatga ttgaagctat tataaaagct tgggataaag cagaatttga agagggacga
     2401 catacacaaa gggttgaaaa aataaacgct ttaagtaaaa ttggaaattg tagagcataa
     2461 tttaatttta tatacaagga aaatattatg gctgattggc aagtttattt gatgatgact
     2521 gcaattgttg cagctatgtt gattgggaaa aaacttatgt tttttgatag taacaaaaaa
     2581 aagagtgatg ataaaaaaat caataaggat gaaaattgat aaatccaaaa attatcgaat
     2641 atatattttc aagtgcatct attcaaaggt ggaatgatta tccaagaatg gttgatttag
     2701 tagaacttga caaacaagca cataaattta taattgctta ttttatagca aaacttgaag
     2761 atgatataaa ttttacacat ctaatagaag ctggaatttt tgagttttta agaagagttg
     2821 ttgttacaga tattagacct gatgttttta gaaaagcttt acaacaaaaa tcaaaagaga
     2881 ttaacacttg ggttgtaggt aaactaaaaa gctctataga aaatattgag aatggaaact
     2941 ttttacaaaa atttgaagaa tttttaaatg atccaaatat gtataaaaaa gagagattta
     3001 tattaaaggc agcttcatat ttatctacaa aatgggaatt ttcaatagtt tatcaaacaa
     3061 gtcaattctt aagcgatata gaggaagtta aaaaagctgt tgatgctgaa attgaagatt
     3121 attatgagct aattggagta agaaaaatag ctctaaataa aaaattagca aaaatagtag
     3181 atttaagtgg aagattaaga tttcaaaaaa gatgggctca aactccaaga attcctgaaa
     3241 cttcagtttt aggacatatg ctaacagttg ccctattttc atatttttac tctttagaag
     3301 taaaagcttg tgacaaaaga ttacaaaaca atttttttac agcgctattt catgatttac
     3361 cagaagcttt aacaagagat attataagcc ctgtaaaata tagtgtagat gaactttcag
     3421 agataattgc tgaatatgaa gttgcaaaaa tagaagatga tattttgcca aatattccta
     3481 taaatatcca agaagagttc tcttatattc tagggatttc aaaaggtata aaagaggagt
     3541 ttgcaaataa agtaaaaata gatgggaata tcacagaagt tgatgatata aataagtata
     3601 atcttgataa atatgaagcc atagatggtt tagctcttaa acagtgtgat aaattatctg
     3661 cttttgttga agcatcttta tctatatctc atggaataaa aagtaaagag ctaatcagtg
     3721 gtaaaaaaga gattttaaaa ggcttaaaag agataaatgg ggttgatttt aaacaaatag
     3781 ctcttgatat tgacagtgag tttggaagta acggacagat tcaaacaact ttagattttg
     3841 aataatcttt tattttatag agtatttata taagtgttat taactgcaga gcaagaagag
     3901 attgtaaata gttcattaga ctcatttaaa ataaatgcag ttgcaggaag tggtaaaaca
     3961 acaactcttc ttgagtatgc aaaaaaaaat tcaaacttaa aaattttata tttagcttat
     4021 aataaatctt tacaaattgc tctaaatgaa aaattaaaag attatcattt accaaatctt
     4081 catataagca caattcactc tttagcatac aataaaacag aagcttacaa atacaaacta
     4141 acacctgaat taaaaacaaa tatcctagaa aaactaatca taaatcatga atttcaagat
     4201 aataaaaaga gctactaccc tagtttagaa tatacaacta ttttaaaaaa ctttataaat
     4261 ttttactgta attcaaattt aatagagctt gatttaaaac tacttgaaga gtttaaaaag
     4321 caaaatgatt ttggggtaaa aatcttagat attttaaata aaaaagagaa aaaactatta
     4381 gaacatttaa aactaactct tagctctatg aagttaggaa aaatagatgc tattcatgac
     4441 ttttatctaa aaatgttcta tcttaataaa aaaatatctt caaatctcaa ttatgattta
     4501 attttaattg atgaagcaca agatataagc gatgtgatga taggaataat tgagaatcaa
     4561 aaatgtagaa aaatatatgt tggagatagt tttcaacaaa tttatagttt cagatatgct
     4621 tcaaatgcac ttaataaaat agatttacca tcttatgaat taact
//
LOCUS       DAOQAN010000026         4513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_24752_length_4513_cov_2.587035, whole genome shotgun sequence.
ACCESSION   DAOQAN010000026 DAOQAN010000000
VERSION     DAOQAN010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 4513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4513
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24752_length_4513_cov_2.587035"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="PKO10_00810"
     CDS             <1..373
                     /locus_tag="PKO10_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HNW65733.1"
                     /translation="LYQEARSYINILYPNDDMVVFYDFDNIHHLLNLAYCLTIHKTQG
                     MEYENALIPMSFSHYIMHNTKLLYTAITRAKSMCFIVGEEEAFKSACKKIEVTIRESV
                     INDLLCNKDKKIETNSLSISV"
     gene            434..1900
                     /locus_tag="PKO10_00815"
     CDS             434..1900
                     /locus_tag="PKO10_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012013042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HNW65734.1"
                     /translation="MITWMQRHKKWLVITIWISTIAFVGAGFVGWGSYDYGSKGGVVA
                     TVGNKEISVEEYQQEYSNLYNQYSQIFGASFNKELAEQLRLKDVAFSQLLQKNLLMSY
                     GNSLGLMVTDEEIAKELINYEEFKVDGKFNKNQYVNVLQQNRMSPQEFETSLKNSILF
                     QKVQALFSIEPTPNEIENISKLLFLEDDITYKILTLNDVDVNSVDENELKKYFDENQN
                     AYKSEVFYDLEIKELPISSSNSTQEDIKNHFDKFKSDYKFEDGKLKSFEEAKEEVIKD
                     LDENFSKKEALTLYLKLKKSEDNFDKKATYSENKIPFSAENVQKIKELKKEEILKPFF
                     ENNRFYIVKLAKINPSVNLTFEQAKAKVIEDFKLNLKAKKLDELAQNSLKDFKGNEAT
                     AVNRASASKIKGLNEQEAIEFLAQLFASSTKEGIAKVGSKVVLYKIIDSRMASYDKAK
                     DSFVRDEIKQVQNSDLLSNLLKKLENSIVIKTFMQAKE"
     gene            1903..3285
                     /gene="ftsA"
                     /locus_tag="PKO10_00820"
     CDS             1903..3285
                     /gene="ftsA"
                     /locus_tag="PKO10_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HNW65735.1"
                     /translation="MNNTNDIFLTINIGSSVTSAVISKPKYDLDNSIEILGFGVQDSV
                     GVNKGLITNIEDVSRTIKDAILQAKESVTETIGTTVVSISGNYTRGIKATGYVTIPNG
                     LVSETDINQAMQMALSNSIILPDYDVVHVIPLFFKVDDQEVENPFNMNGSKLEASVYI
                     VTAKRNALINIKSALRIFGIDDVRFVLDSYAASLAVLDEQQKRIGAVVINLGASTTEF
                     VYHKGNSIIYNGFIPVGSKNITNDLSLMLNTPLRTAEKIKIEYGSLIKDYFGNGEVGP
                     SNVGVSQINDEEYFKEFPLGHIQTIIHARVEELLVLVKNELKKNALLDNIGSGIVLTG
                     GMSQLGGIKELTKNIFEKIPVTIATPKNIPNSFRVSFDEPHMATVVGLIMFALGMNRG
                     YQLDSSKKLIRPIRKGQVNDKFIAPQTPHIGQAGPDMRIRTNDTILDPLPKDKKKKGL
                     GLWKKIEEWF"
     gene            3285..4442
                     /gene="ftsZ"
                     /locus_tag="PKO10_00825"
     CDS             3285..4442
                     /gene="ftsZ"
                     /locus_tag="PKO10_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HNW65736.1"
                     /translation="MENSSSHNLFRVDDIMIKEDMPTKALSNNLPKIAVIGVGGGGCN
                     MINYMIEEGTHMIDLIVANTDLKVLHISKAPKKIELGPMLTNGFGAGMDPEVGRNSAL
                     ESYDEIKETLKGADIVFVAAGLGGGTGTGAAPIIAKAAREVGALTVSVVTKPFGFEGR
                     MRAALANLGLEELKKVSDSLIVIANDKLREAVDETIGIKNAFKVTDNILYQAVNGMSQ
                     VILNPGSGNDINADFADVKTIMKHKGIALMGIGKAKGDEATSRALDNAINSPLLEKVP
                     LDGAKGILIHFTISPEISLFAIEDVMNNINQRVDINAQIIFGTTTDTDFERDEVKITI
                     IATGFEAKNEIKEEQKESDENEIEAIKVEAVESTLDTPPLMRGYTVEYPLH"
BASE COUNT         1766 a          496 c          800 g         1451 t
ORIGIN      
        1 tttataccag gaagccagat cttatattaa tattttatat ccaaatgatg atatggtagt
       61 attttatgac tttgataata ttcatcatct tttaaattta gcatattgtc taacaattca
      121 taaaacacaa ggaatggagt atgaaaatgc tttaattcct atgagctttt cacactatat
      181 aatgcataat acaaaactac tttatacagc aataacaaga gcaaaaagta tgtgttttat
      241 tgttggagaa gaagaagctt ttaaaagtgc ttgtaaaaaa atagaagtga caataagaga
      301 gagtgttata aatgatttat tgtgcaataa agataaaaaa atagaaacaa atagtttatc
      361 tataagcgtt taaataacat acaagcaaga tttttgtatt ataatctcta aatttttttt
      421 aagggtttat tttatgataa cttggatgca aagacataaa aagtggttgg ttattacgat
      481 ttggattagt actattgcct ttgtaggtgc tggatttgta ggatggggat catatgacta
      541 tggtagtaaa ggtggcgttg ttgcaactgt tggaaacaaa gagattagtg tagaagagta
      601 ccaacaagag tattcaaatc tttataatca atattcacaa atttttggag catcttttaa
      661 taaagagtta gcagagcaat taagattaaa agatgttgca ttttctcaac ttttacaaaa
      721 aaatcttctt atgtcttatg gtaactcttt aggtttgatg gtaactgatg aagagattgc
      781 aaaagagttg attaattatg aagagtttaa agtagatggt aaattcaata aaaatcaata
      841 tgtaaatgtt ttacaacaaa atagaatgtc acctcaagag tttgaaacta gtcttaaaaa
      901 ttcaatactt tttcaaaaag ttcaagctct ttttagtata gaaccaactc caaatgagat
      961 agaaaatatt agtaaactac tatttttaga agatgatatt acttataaaa tattaacttt
     1021 gaatgatgtt gatgtaaata gtgttgatga aaatgaactt aaaaaatatt ttgatgagaa
     1081 tcaaaatgca tataagagtg aagtttttta tgatttagag ataaaagagc ttcctatttc
     1141 ttcttctaac tctacacaag aagatataaa aaatcatttt gacaagttta aaagtgatta
     1201 taaattcgaa gatggaaagt taaaatcttt tgaagaagca aaagaagaag taataaaaga
     1261 tttagatgag aattttagta aaaaagaggc tcttactctt tacttaaaac ttaaaaaatc
     1321 tgaagataac tttgacaaaa aagcaactta ttctgaaaat aaaattccat tttcagctga
     1381 aaatgttcaa aaaattaaag agttaaaaaa agaagagatt ttaaaaccat tttttgaaaa
     1441 taatagattt tatatagtaa aactagcaaa aatcaaccct tctgttaatt taacttttga
     1501 gcaagcaaaa gcaaaggtaa ttgaagattt taaacttaat ttgaaagcaa aaaaattaga
     1561 tgaattagca caaaatagtt taaaagattt taaaggaaat gaagcaacag cagtaaatag
     1621 agcaagtgct tctaaaatta aaggattaaa tgagcaagaa gcaatagagt ttttagcaca
     1681 actttttgct agttctacaa aagagggaat tgcaaaagtt ggttcaaaag ttgttttata
     1741 taaaataatt gactcaagaa tggcttctta cgacaaagca aaagatagtt ttgtaagaga
     1801 tgagataaaa caggttcaaa attctgattt attgtcaaat ttacttaaaa aacttgaaaa
     1861 tagtattgta ataaaaacat ttatgcaggc aaaggagtag ttttgaataa tacaaatgat
     1921 atttttctta caataaatat tggttctagt gttacaagtg cagtaatatc taaaccaaaa
     1981 tatgatttag ataatagtat tgaaatttta ggttttggag ttcaagatag tgttggagta
     2041 aataaaggac ttataacaaa tattgaagat gtttcaagaa ctataaaaga tgcaatactt
     2101 caagcaaaag agagtgtaac agagactatt ggaacgactg ttgtatctat ttctggaaat
     2161 tatacaagag gaatcaaagc aactggatat gtaactattc caaatggact tgtttctgaa
     2221 acagatataa atcaagcaat gcaaatggct ctttcaaact caataatttt acctgattat
     2281 gatgtagttc acgtaatacc actatttttt aaagtagatg atcaagaagt tgaaaaccca
     2341 tttaatatga atggttcaaa gcttgaagca tctgtttaca ttgtaactgc aaaaagaaat
     2401 gcactaatta atattaaatc tgcactaaga atttttggta tagatgatgt tagatttgtg
     2461 cttgactctt atgctgcttc tttggcagtt ttagatgagc aacaaaaaag aataggtgct
     2521 gttgttatta acttaggtgc ttctacaact gagtttgtat atcacaaagg aaactctata
     2581 atttataatg gatttattcc tgttggttca aaaaatatta caaatgatct atctttgatg
     2641 ttaaatacac ctcttagaac agctgaaaag ataaaaattg agtatggttc tttaatcaaa
     2701 gattactttg gaaatggaga agttggacct tcaaatgttg gagtttcaca aataaacgat
     2761 gaagagtatt ttaaagagtt tcctcttggt catattcaaa caattattca tgcaagagta
     2821 gaagagcttt tagttttagt taaaaatgag ttaaaaaaga atgctttact tgataatatt
     2881 ggttcaggaa tagtattaac aggtggaatg agtcaattag gtggaataaa agagctgaca
     2941 aaaaatattt ttgagaagat tccagtaaca attgctactc caaaaaacat tccaaattca
     3001 tttagagtta gttttgatga gccacatatg gcaacagttg ttggattgat tatgtttgct
     3061 ttaggaatga atagaggtta tcaattagat tctagtaaaa aattaataag acctattaga
     3121 aaaggtcaag ttaatgataa gtttattgca ccacaaactc cacatattgg acaagctgga
     3181 cctgatatga gaataagaac gaatgataca atcttagacc ctttaccaaa agataaaaag
     3241 aaaaaaggtc ttggactttg gaaaaaaatt gaggagtggt tttaatggaa aatagtagca
     3301 gtcacaatct atttagagta gatgatataa tgataaaaga ggatatgcct acaaaagcac
     3361 tatcaaataa tcttccaaaa attgcagtaa ttggtgttgg aggtggtggt tgtaatatga
     3421 ttaattatat gattgaagaa ggtactcata tgattgattt aatagttgca aatacagatt
     3481 taaaagttct tcatatttca aaagctccaa aaaagattga attaggacct atgcttacaa
     3541 atgggtttgg tgctggaatg gatccagaag ttggaagaaa ctcagcgctt gaaagttatg
     3601 atgagataaa agagacttta aaaggtgctg atattgtttt tgttgctgct ggattaggtg
     3661 gaggtacagg aacaggagct gctcctatta ttgcaaaagc agccagagaa gttggagctt
     3721 tgactgtttc tgttgttact aaaccttttg gttttgaagg aagaatgaga gctgctttgg
     3781 ctaatttagg tcttgaagag cttaaaaaag ttagtgattc tttaattgtt attgcaaatg
     3841 ataaactaag agaagctgtt gatgagacaa ttggtataaa aaatgctttt aaagttacag
     3901 ataatatttt atatcaagct gtaaatggaa tgagccaagt tatattaaat ccaggtagtg
     3961 gaaatgatat aaatgccgat tttgcagatg ttaaaactat tatgaagcat aaaggtatcg
     4021 ctttaatggg aattggaaaa gcaaaaggag atgaggctac aagtagagct ttagataatg
     4081 ctataaactc tcctttacta gaaaaagtac ctttagatgg tgcaaaagga attttaattc
     4141 actttacaat aagtccagaa atatcacttt ttgctataga agatgttatg aataacataa
     4201 accaaagagt tgatataaat gctcaaatta tttttggaac tacaacagat acagattttg
     4261 aaagagatga agtaaaaatt actatcatag caactggatt tgaagctaaa aatgagataa
     4321 aagaggaaca aaaagagagt gatgaaaatg agattgaagc tataaaagtt gaagcagttg
     4381 aaagcactct tgatactcca cctcttatga gaggttatac agtagagtat ccacttcact
     4441 aaaattattt aataaaatat ctaaggatac aaactcaaat atttgagttt gtatcctttt
     4501 tttttatatt tcg
//
LOCUS       DAOQAN010000027         4421 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_25505_length_4421_cov_2.863949, whole genome shotgun sequence.
ACCESSION   DAOQAN010000027 DAOQAN010000000
VERSION     DAOQAN010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 4421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4421
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25505_length_4421_cov_2.863949"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2204)
                     /gene="leuS"
                     /locus_tag="PKO10_00830"
     CDS             complement(<1..2204)
                     /gene="leuS"
                     /locus_tag="PKO10_00830"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HNW65737.1"
                     /translation="MEYLAKDIEKKWQKFWIDNKSFEPSDDFSKPKKYILSMFPYPSG
                     RIHMGHVRNYCLGDAFARHFRKNDFNVLHPIGWDSFGMPAENAAIKNKLHPKKWTYEN
                     IDYMRNELRSLGLSFSENQEFATSDELYTKYEQEFIIKMYEAGVLYRKSATVNWCNDC
                     QTVLANEQVVEGCCWRCDNEIVQKEMPGYYVAITKYAQTLLDDLKTLENSWPSQVLTM
                     QENWIGRSEGLEFKFDVTKETRAKLDKMFANFSVFTTRPDTIYGVSYTALSPEHPIVK
                     YILEKELLPKNKLNAIKNMQKVPQKDRAIQEKEGIDLEIEAIHPLTGQKVPVWVANFV
                     LSSYGEGAVMAVPAHDQRDFEFAKKYDLPIKQVIVGDDGLIEKQTAAYTGDGVLINSE
                     SFTGLKNSDAKNAIMYHFEQNSNGSKKVNFKLRDWGVSRQRYWGAPIPFVHCKTCGLV
                     PEKLENLPVALPDDVEITGEGNPLDLHPTWKHCSCPKCGQSAIRETDTLDTFVQSSWY
                     FLRYATNHKKWQTEGISKEDSDYWMDVDQYIGGIEHAILHLLYARFFTKVLKDLGYTN
                     SNEPFKRLLTQGMVLKDGAKMSKSKGNVVDPDILIEKYGADTARMFILFAAPPTKELE
                     WNDSAVEGAYKFIKRFYERAINITSDGLKEFKNISQDSLSKDEKEARKKVYEALNKSN
                     EVLTKTYAFNTLIASCMEAMNYLSSQKNEAIWAEAYYILTNILEPIIPHACW"
     gene            complement(2204..2548)
                     /locus_tag="PKO10_00835"
     CDS             complement(2204..2548)
                     /locus_tag="PKO10_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6394 family protein"
                     /protein_id="HNW65738.1"
                     /translation="MDWGKVTYIFLTLMSLTTTAGFLYEPNIIALFIAGGVNVISTIL
                     KIGVKNLLAAELLACSLVADLHLIPAFLVFVFTGNMKLVIALAIGAVIANIVSMILSM
                     VESAKNKEKEDY"
     gene            2703..3455
                     /gene="motA"
                     /locus_tag="PKO10_00840"
     CDS             2703..3455
                     /gene="motA"
                     /locus_tag="PKO10_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HNW65739.1"
                     /translation="MDLSVLLGLIGAITSISVGVILEGGNPAGVLHISSFIIVIPTAM
                     LAAVVATDSKYVKAAFKEFKNIFKKSPVNFEARIDELVEYAITVKKQGVLALEKDVQC
                     LNHQFLKEALSMVVDGSKEEQIEEQLEPVIEATEEYYHGASHFWLHAGETSPTIGLVG
                     AVFGLILALQKLDDPPAMAAGIAGAFTATVMGIAGSYIFLGPWGVKLKAKGHLVVKEQ
                     YLILAACKGMARGDAPGELKLKLSKMVTPMPL"
     gene            3467..4210
                     /gene="motB"
                     /locus_tag="PKO10_00845"
     CDS             3467..4210
                     /gene="motB"
                     /locus_tag="PKO10_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HNW65740.1"
                     /translation="MSKKKKCECPAGEKWAVPYADFLSLLLALFIALYALASVNMEKQ
                     KALKEEFIKIYKFPSANIVEEQTKQEKAMTDEPSDDNVEGKKVIVHTLENKDEQEQDQ
                     NKGANLIELPNGSLMSVPAHLAFESGKSEITSVFANDFLSNLAKLINAMPEDTEINVK
                     GYAQDSEVKNSRHKDALELSTARANNVIRELVKYNVKPSRLYSSGFGSNKESTLKDKS
                     VVVFELHSNGQIQSEEDLNLETIFNKMKE"
     gene            4210..>4421
                     /locus_tag="PKO10_00850"
     CDS             4210..>4421
                     /locus_tag="PKO10_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW65741.1"
                     /translation="MQSKKKFNTLLPTLKKRCCFLKFIDFEPIVKYGSIFIILVSIFI
                     QSSNIIQNKNLLLSIFSLFFNLFFLL"
BASE COUNT         1478 a          717 c          637 g         1589 t
ORIGIN      
        1 tcccaacatg catgagggat aattggctct aaaatatttg ttaaaatata gtaagcttct
       61 gcccaaatag cttcattttt ttgtgatgat aaatagttca tagcctccat acaagaagca
      121 attaaagtat taaaagcata tgtttttgtt aaaacttcat ttgacttatt taaggcttca
      181 taaacttttt ttctagcctc tttttcatct ttacttaaac tatcttgaga tatgttttta
      241 aactctttta aaccatctga agttatatta atagctcttt cataaaatct ttttataaat
      301 ttatatgcac cttcaactgc actatcattc cactctaact cttttgttgg tggtgctgca
      361 aaaagtataa acattcttgc tgtatctgct ccatattttt caattaaaat atcaggatct
      421 acaacatttc ctttactttt actcattttt gcaccatctt ttaaaaccat accttgtgtt
      481 aaaagtcttt taaatggttc attggaattt gtataaccta aatcttttaa aacttttgtg
      541 aaaaatcttg catataaaag gtgtaaaata gcatgttcta ttccaccaat atattgatca
      601 acatccatcc aataatcact atcttctttt gaaattcctt ctgtttgcca tttcttatga
      661 tttgttgcat atcttaaaaa gtaccaagat gattgcacaa aagtatctaa tgtatctgtt
      721 tctcttatag cactttgacc acattttggg caagagcagt gtttccaagt aggatgaaga
      781 tcaagaggat ttccctctcc tgtaatttct acatcatcag gaagagctac aggaagattt
      841 tctaattttt caggaaccaa gccacaagtt ttacagtgaa caaatggaat aggagcaccc
      901 caatatctct gtcttgaaac tccccaatct ctaagtttaa aatttacttt tttacttcca
      961 tttgaatttt gttcaaagtg atacattatt gcattttttg catcactatt ttttaagcca
     1021 gtaaaacttt cactatttat taaaactcca tctcctgtat aagcagctgt ttgtttttct
     1081 attagtccat catctccaac aataacttgt tttattggta aatcatactt tttagcaaat
     1141 tcaaaatctc tttggtcatg tgcaggaact gccataacag caccttcacc ataagagctt
     1201 aatacaaaat ttgcaaccca aactggtact ttttgtccag ttaatggatg aattgcttca
     1261 atttctaaat caattccctc tttttcttgt attgctctat ctttttgagg tactttttgc
     1321 atatttttaa tagcatttaa tttatttttt ggcaacaact ctttttctaa tatgtatttt
     1381 acaattggat gctctggaga aagtgctgta tatgaaactc cataaatagt atcaggtctt
     1441 gtagtaaata cactaaaatt tgcaaacatt ttatcaagtt ttgctcttgt ctcttttgtt
     1501 acatcaaatt taaactctaa tccttcgctt cttccaatcc aattctcttg cattgttaaa
     1561 acttgtgaag gccatgaatt ttcaagtgtt ttcaaatcat caagcaaagt ttgtgcatat
     1621 tttgtaattg ctacatagta tccaggcatc tctttttgaa caatttcatt gtcacatctc
     1681 cagcaacacc cctcaacaac ttgctcatta gctaaaactg tttgacaatc attacaccaa
     1741 tttacagttg cactttttct atataaaact ccagcttcat acattttaat tataaactct
     1801 tgctcatatt ttgtataaag ctcatcacta gttgcaaact cttgattttc actaaaagat
     1861 agacctaaac ttcttagctc atttctcata taatctatat tttcataagt ccattttttt
     1921 ggatgaagtt tgtttttaat agcagcattt tcagctggca ttccaaaact atcccaacca
     1981 attggatgaa gtacattaaa atcatttttt ctaaaatgtc tggcaaaagc atcacctaaa
     2041 cagtaatttc taacatgtcc catgtgaatt cttccactag ggtatggaaa catacttaat
     2101 atatattttt ttggttttga aaaatcatca cttggctcaa aagatttatt atcaatccaa
     2161 aatttttgcc actttttttc aatatccttc gccaaatact ccattaataa tcttcctttt
     2221 ctttattttt tgcactctct accatagaaa gtatcattga tacaatattt gcaattacag
     2281 caccaattgc aagagcaata actaatttca tatttcctgt aaaaacaaat accaaaaatg
     2341 caggaatcaa gtgtaaatca gcaactaatg aacaagccaa aagctcagct gcaagaagat
     2401 tttttacacc aatttttaaa attgtagata ttacattaac tccacctgct ataaaaagag
     2461 ctattatatt tggttcatac aaaaaaccag ccgttgttgt taaagacatt aatgttaaga
     2521 aaatataggt taccttaccc cagtccattt ttttcctttt aactatttaa aataaagtat
     2581 gtatattagc taaaattgta taaaaaacta attttaacta tattgcaaac tttatttata
     2641 gattacagca cttttttaat ttactttaga tattataaat taattttttt ttaggaaaac
     2701 aaatggattt atctgtactg ttaggattaa taggtgctat aacatctatt tccgtgggag
     2761 ttattttaga aggtggaaat cccgctggag ttcttcatat ctcctcattt attatagtta
     2821 tacccactgc catgctagcg gctgttgttg caactgattc caaatatgta aaagcggctt
     2881 ttaaagagtt taaaaatata ttcaaaaagt cacctgtgaa ttttgaagca agaattgatg
     2941 agcttgttga gtatgcaata acggttaaga aacagggggt tttagcctta gaaaaagatg
     3001 ttcagtgttt aaatcatcaa ttcttaaaag aggctttaag tatggttgtt gatggaagca
     3061 aagaggagca aattgaagaa caattagaac ctgtaattga agcaacagaa gagtactatc
     3121 acggagctag tcacttttgg ttgcatgcgg gggaaacatc tcctactatt gggcttgttg
     3181 gtgctgtttt tggtctaatt ttagctttac aaaaacttga tgatcctcct gctatggcag
     3241 ctggaattgc tggagcattt acagcaaccg ttatgggaat tgctggttcg tatatttttt
     3301 taggaccttg gggagttaaa ttaaaagcta aagggcattt agttgtaaaa gagcaatact
     3361 taatacttgc tgcttgtaaa ggaatggcac gaggtgatgc accaggtgaa cttaaattga
     3421 agttatctaa aatggtaact ccaatgcctt tataggattt ttttaaatgt ctaaaaaaaa
     3481 gaagtgtgag tgcccagctg gtgagaagtg ggcagttcca tatgcagatt ttttaagtct
     3541 tctactagct ctttttattg ctttatacgc tttggcttct gtaaatatgg agaaacaaaa
     3601 agcattaaaa gaggagttta taaaaattta taaatttcca tctgcaaata tagttgaaga
     3661 gcaaacaaaa caagaaaaag ctatgacaga tgaaccttct gatgataatg tcgagggaaa
     3721 aaaagttata gttcatactt tagagaataa agatgaacaa gaacaagatc aaaataaagg
     3781 tgcaaattta atagagcttc caaatggttc tttaatgagt gttccagcac atttagcttt
     3841 tgagagtgga aaatcagaga ttacatctgt ttttgcaaat gattttttga gtaacttagc
     3901 taaattaata aatgcaatgc ctgaagatac tgaaataaat gtaaaaggtt atgcacaaga
     3961 tagtgaggtt aaaaactcta gacacaaaga tgctttagag ctttcaacag caagagcaaa
     4021 taatgtaata agagagttag taaaatacaa tgtaaaacct tcaaggctat actcaagtgg
     4081 ttttggaagt aataaagagt caactctaaa agataaaagt gtagttgtgt ttgaacttca
     4141 ttcaaatggt caaattcaaa gtgaagaaga tttaaattta gagacaattt ttaataaaat
     4201 gaaagagtaa tgcaaagtaa aaaaaagttt aatactcttc ttcctactct aaaaaagagg
     4261 tgctgttttt taaagtttat agattttgaa ccaatagtaa aatatggttc aatctttata
     4321 atcttagtat ctatttttat tcaatcttca aatataattc aaaataaaaa cttactatta
     4381 agtatttttt cactcttttt taatctattt tttctattaa t
//
LOCUS       DAOQAN010000028         4346 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_26166_length_4346_cov_6.099511, whole genome shotgun sequence.
ACCESSION   DAOQAN010000028 DAOQAN010000000
VERSION     DAOQAN010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 4346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4346
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26166_length_4346_cov_6.099511"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(189..1370)
                     /locus_tag="PKO10_00855"
     CDS             complement(189..1370)
                     /locus_tag="PKO10_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HNW65742.1"
                     /translation="MSRKMTQYSAEFKTKIVLEVLKGDKTINEIASEYNIIPKNIQNW
                     KNIFLANAVVAMEPAKAVKEYKDEIEKLKAKNDEYAKIVGKLTVENNWMSGKLKSLDS
                     KKRQDIVEFKLSDISITRQSELVGVARSGLYYLPKINEEELAIKQQIQAIYNEIPIYG
                     YLKVHKKLLEDGFIISPNTVQKYRKDLGLKAVLAVRAPYTTKGHKEHPIYSYKLKGLE
                     ITRANQVWSTDITYIRIKGGFVYLSAVIDWYSKAVLAWRISNTMDTDLVMSVLNEALS
                     LYPKPEIFNTDQGSQYTSYIHTDTLKAHGITISMNGAGRSVDNICIERFWRSAKVEKI
                     YLNEYDRVSVLKEDVKDYINFYNYKRFHESLQYKKPIEVYFNSMKINEQNYNSKQISV
                     A"
     gene            1843..3387
                     /gene="istA"
                     /locus_tag="PKO10_00860"
     CDS             1843..3387
                     /gene="istA"
                     /locus_tag="PKO10_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008340375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HNW65743.1"
                     /translation="MEKIAEVIRLHFKLDLSVRQTSSALNIARSSVGDYCQKFKSLNL
                     DIDNFLKLSVEKQEELLFAKTSNIIPLDNKKVLPDCNYLHNELKKRKKTGVTLALLHE
                     EYKESNPNGYYSYTQFREHYKRYLNTINPSMKQIHLVAEKMFIDYSGVTVPIVNKKTG
                     EIVKAEIFVAVLGASGYTFVDASYSQKQKDFINSNVKALEFFQGCPRVIVCDNLKSAV
                     IKHSKKDGIVLNESYAAFAKYYNMVIEPARPYKPKDKAKVEQGVQAIQRWILATIRYQ
                     RFFSIDELNDAISPLLDKYNAKIVKRFNKSRLELFNELDLPNLQSLPKQRYIYKEHKE
                     ATVNVGYHISLDNCEYSVPFEYLSKKVQLRYSSSSVEIYYKDNLIATHPKLHFAGSNS
                     TKVEHMPKSHQYQTLKTNPGSFLNWANNIGVNTVYWVKKELKSVKHPPNVYNKLNAVL
                     SMSKIYGKKELDLALQYALQNGLSRTSSIRSILDKKLYLQKEISDMQSYAIINNHENL
                     RGNIYK"
     gene            3432..4190
                     /gene="istB"
                     /locus_tag="PKO10_00865"
     CDS             3432..4190
                     /gene="istB"
                     /locus_tag="PKO10_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008338275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HNW65744.1"
                     /translation="MNSSIVINKILELNYKGFKEAYIRQIEDINYNQLSFDERLYDLL
                     DSEYLYLKNKRVEMNFKLSRIKDKQAVIEEIDYLPQRKLNKSLILSLASMNFLKAKQN
                     IIINGKTGTGKSFLAQAIGNRAIHEGFTVYYIRTSTLLEEIKLSRIDGSYTNLVKRFA
                     RYKLLILDDFGVSPISVDDATNLFEIIETRNQLSSTIITSQLPVKDWYGYLQNNTVAD
                     AILDRVVHSSHRIEIEGDSLRPKYSNLNQKFEEN"
BASE COUNT         1660 a          627 c          562 g         1497 t
ORIGIN      
        1 ttattttaca ttctactgag acacaagttt cagtatatga aatagacgat aataatcaaa
       61 tttctatttc atcaaatcac attaaattta aaagtattca aattagagaa agtgaggatg
      121 tatttataat accccaaaat gtcaagacaa ctttttctaa aatcttattc tcttaagtac
      181 ctgttactct atgctacact tatctgtttt gaattataat tttgttcatt tattttcata
      241 ctattaaaat atacctctat aggtttttta tattgtaaac tttcatgaaa tcttttataa
      301 ttataaaagt ttatataatc ttttacatcc tcttttagaa ctgatactct atcatactca
      361 ttaagatata ttttttctac tttagcactc ctccaaaatc tttcaataca aatattatca
      421 acacttctac cagctccatt cattgaaata gtgataccat gtgcttttag tgtatctgta
      481 tggatatagc ttgtatattg actaccttga tcggtattaa atatttcagg cttaggataa
      541 agtgataatg cttcgtttaa aacactcata actaaatctg tatccatagt gtttgatatc
      601 ctccatgcca acactgcttt actataccaa tcaattactg cagataaata tacaaatcca
      661 cccttgatac ggatataggt aatatcagta gaccaaactt gattagctct tgtaatctct
      721 aagcctttta atttataaga gtatattgga tgttctttat gccctttagt tgtatacggt
      781 gcacgaacag ctaatacagc ttttaaccct aaatcttttc gatacttttg cactgtattt
      841 ggtgatatta taaaaccatc ttctaaaagc ttcttatgaa cctttaggta tccatatatt
      901 ggtatctcat tatagatagc ttgtatttgc tgttttatag ccaattcttc ttcatttatt
      961 tttggaagat aatacaaacc actccttgca acacctacta gttcactttg tcttgttata
     1021 gaaatatcac ttagcttgaa ctcaacaata tcttgtcttt ttttagagtc caagctcttt
     1081 aactttcccg acatccaatt attctccact gttaatttac caactatttt tgcatactca
     1141 tcatttttgg cttttagctt ctctatctca tctttatact ctttgactgc ctttgctggt
     1201 tccatagcta caacagcatt tgctaaaaat atatttttcc aattttgaat attctttgga
     1261 ataatattat attcacttgc aatctcatta atagtcttat caccttttaa aacttccaat
     1321 actattttgg ttttaaattc tgcactatat tgtgtcattt ttctactcat ttttgaccct
     1381 tctattttat ctttaaattt tactctaaga gaataaaact ttacaaaaaa ttgttcaatt
     1441 ttcttggggt attataattt tcatctttat ttataaataa aatatcatct tttaatttaa
     1501 tctttgaaaa tattgtatta ttatcattat ataactcaaa ttgtacttct aactcattct
     1561 ttgacaaaag tttaatattc atattatcat tcaataattt atgtattata tatacttcaa
     1621 actgtagtat atcaatcttt gcaaaaatcg gtctaaaccg gacaacgatg caagagttag
     1681 accggacgca aaaacggagt ttaaccggac agtaaaacgg tgtagcaccg gacactaaaa
     1741 ggtaaaaacc gataattaag attaaattca gcaaaaggga ttttctaaat ttatatactt
     1801 caaaaatatt aaatttttgg agtagaaatg aggaaaataa aaatggaaaa aattgcagag
     1861 gtaataagac tgcattttaa attagatttg tcagtaagac agacttctag tgcattaaat
     1921 atagctagga gtagtgttgg agattattgt caaaagttta aatctttaaa tttagatatt
     1981 gataattttc taaaattaag tgttgaaaaa caagaagaac ttctctttgc taaaacttca
     2041 aatatcatac cactagataa taaaaaagta cttccagatt gtaattactt gcataatgaa
     2101 ttaaaaaagc gtaaaaaaac gggtgtaacc ttagctttgc ttcatgaaga gtataaagag
     2161 tcaaatccaa atggatatta tagttatact caatttagag aacattataa aagataccta
     2221 aatacaataa atccttcaat gaaacagatt catcttgtag cagagaagat gtttattgat
     2281 tatagtggag ttactgttcc tattgtaaat aaaaaaacag gtgagattgt aaaagcagaa
     2341 atatttgtag cagttcttgg agctagtgga tatacctttg tagatgcttc atattcacaa
     2401 aaacaaaaag atttcataaa ctctaatgta aaagctttag agttttttca aggatgccca
     2461 agagtaattg tttgtgataa tttaaaaagt gctgttataa aacactcaaa aaaagatggg
     2521 attgtattaa atgaaagtta tgccgcattt gcaaaatatt ataatatggt aatagaacca
     2581 gcacgtccat acaaacccaa agataaagca aaagtagaac aaggagttca agctattcaa
     2641 agatggatat tagcaaccat taggtaccaa agattttttt caattgatga attaaatgat
     2701 gccatttcac ctttattaga taaatataac gcaaaaattg taaaaagatt taataaaagt
     2761 agattagaac tttttaatga attagatctt ccaaatttac aatcacttcc aaaacaaaga
     2821 tatatttata aagagcataa agaagctact gtaaatgttg gatatcatat aagcttagat
     2881 aattgtgaat actctgttcc atttgaatat ttatcaaaaa aagttcaact tagatactca
     2941 agctcatctg ttgagattta ttataaagat aatttaatag caacacatcc caaactacac
     3001 tttgcaggat caaattccac aaaagttgaa catatgccaa aatcacatca atatcaaact
     3061 ctaaaaacaa atccaggaag ctttttaaat tgggcaaata atataggagt aaatacagtt
     3121 tattgggtaa aaaaagaact aaagagtgta aaacatccac caaatgtcta taataaacta
     3181 aatgctgttt tatctatgtc aaaaatatat ggaaaaaaag agttagattt agcacttcaa
     3241 tatgcattac aaaatggttt aagtagaaca tcatctatta gatcaattct tgataagaag
     3301 ctttatttac aaaaagaaat ttcagatatg caatcttatg caattataaa taatcatgaa
     3361 aatcttcgag gaaatatata caaataaaaa tattctgttt ttacagataa aaattaaaag
     3421 gaaaaattaa aatgaatagt tcaatagtta taaataaaat attagaatta aactataaag
     3481 gttttaaaga agcctatata aggcaaatag aagatataaa ttataatcaa ctaagctttg
     3541 atgaaagatt atatgatctg cttgatagtg aatatttgta cttaaaaaat aaaagagtgg
     3601 aaatgaattt taaactctca agaataaaag ataagcaagc tgttattgaa gagatagatt
     3661 atttaccaca aagaaaacta aataaatcac taatactttc acttgcttct atgaattttt
     3721 taaaagctaa acaaaatatt attataaatg gaaaaacagg tactggaaaa tcttttttag
     3781 cgcaagctat aggcaataga gcaatacatg aaggatttac agtttattat atcagaactt
     3841 caactttact tgaagagata aaactttcaa gaatagatgg tagttataca aatttagtaa
     3901 aaagatttgc aagatataaa cttctaatac ttgatgactt tggtgtatca cctatatctg
     3961 ttgatgatgc aacaaattta tttgaaataa ttgaaacaag aaatcaatta tcatcaacaa
     4021 tcataacttc acaacttcca gttaaagatt ggtatggtta tttgcaaaat aatactgttg
     4081 cagatgcaat acttgataga gtagtacact catctcatag aattgaaatt gaaggtgatt
     4141 ctttaagacc aaaatactca aatttaaatc aaaaatttga agaaaattaa ttctaccgga
     4201 cacaatatgt tataattaca ctctagaaaa tctcattgca aaagtgtccg gtacaactcc
     4261 ggcatcactg tccggtagct ccgatatatg cattcaaaga aaagatgaga taaaaaatct
     4321 attaaaagag aaaaactttt tagatg
//
LOCUS       DAOQAN010000029         4317 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_26472_length_4317_cov_3.430080, whole genome shotgun sequence.
ACCESSION   DAOQAN010000029 DAOQAN010000000
VERSION     DAOQAN010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 4317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4317
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26472_length_4317_cov_3.430080"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..748)
                     /gene="aspS"
                     /locus_tag="PKO10_00870"
     CDS             complement(<1..748)
                     /gene="aspS"
                     /locus_tag="PKO10_00870"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HNW65745.1"
                     /translation="MRTHYNTSVKENLIGQKVTVAGWVNSRRDHGGIIFIDLRDKSGL
                     VQLVADPQDCKDALVVAETVRDEYVLIATGTVRARGKGLENPNLETGKIEIILENLVI
                     ENRSKAMPFDINDEKVNDEIKLRNRFLELRSKKSFDIFQLRSKATIQARNTLDELGFL
                     DVETPILTKSTPEGARDYLVPSRVHAGEFYALPQSPQLFKQLLMVAGFDRYFQIAKCF
                     RDEDLRADRQPEFTQIDMELSFVDVDDVLEV"
     gene            complement(793..1971)
                     /locus_tag="PKO10_00875"
     CDS             complement(793..1971)
                     /locus_tag="PKO10_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW65746.1"
                     /translation="MKHLLILIFMAFLVAGCTQKVVKLKMPNQNVPVKKVEEDKKTVV
                     EEFISNDSVIQEEIINNNGSKNEITQEENSEVLASSEPEIKFDSTKANLKIAFVYPSS
                     LVSKYAKNSINTVAAYLTFLNANYDLVVIDSQNESADSINNAFNKVKEEGITKVIALY
                     TPNAINNLNTMSLNDIMVYLPLIEKKDSLSQNDNLIFGSISYEDQLKKLSYYSDGPNV
                     LFYQNTYLGNKLKNSYENVVSYTTARKEIKSGETNFKNIVVDSRLRNSSIFLNVDIVK
                     SSLIMSQLRANDIYPKFIFSTQINFDPMLMTLTQDKDREKMVLANSIEKIDNKLRDEI
                     LNFGGNINFEWVDYSTLVGANYLVNGNNNLIPTKIVENQAVYTPRLFKSTEYGFVEIK
                     "
     gene            complement(1973..2638)
                     /locus_tag="PKO10_00880"
     CDS             complement(1973..2638)
                     /locus_tag="PKO10_00880"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNW65747.1"
                     /translation="MTNKVKNQVLKYLYNQKLFGIKYHSDLNINFYSSCDFALPNSLE
                     ELKKSVTNCYLCDLSKRRKHTLFGYGSQNSKIMFICDEPSKSEDDLGSFFVGNSGDML
                     AKMIEGSLKIKKEEVYTTNLVKCRGTKEASFQNFESCNCYLLKQIEVIKPKIIVAVGE
                     RVHDYLLKTSGEFLKNRGKVLSFNSAILVPIFSPLYLLKNPSLKKDTYLDMLKIKNLY
                     EES"
     gene            complement(2718..2936)
                     /gene="infA"
                     /locus_tag="PKO10_00885"
     CDS             complement(2718..2936)
                     /gene="infA"
                     /locus_tag="PKO10_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018060652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HNW65748.1"
                     /translation="MAKDDVIVVDGKVVEALPNAMFRVQLENGHIVLCHISGKMRMHY
                     IKILPNDTVTVEITPYSLDKGRIVHRKK"
     gene            complement(2946..3710)
                     /gene="map"
                     /locus_tag="PKO10_00890"
     CDS             complement(2946..3710)
                     /gene="map"
                     /locus_tag="PKO10_00890"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HNW65749.1"
                     /translation="MSIALRKPEEIEKLFVANQAVAKTLKYLEENVKAGMTLKEVDAM
                     GEKFILSLGARPAFKGLYGFPNAVCTSLNEVIIHGIPSDTVLKEGDILGLDIGTEVDG
                     WYGDSAITMPIGDISKKDEELIACAKDALYYAIDIIEDGMRFKELSKAIEDFITSRGY
                     QPLVRFCGHGIGRKPHEEPEIPNYLEHGGTKSGPKIKNGMVFCIEPMICQKDRNPVIL
                     KNGWDVVSADGLRGSHYEHTVAVVDGRAVILSNREN"
     gene            complement(3712..>4317)
                     /locus_tag="PKO10_00895"
     CDS             complement(3712..>4317)
                     /locus_tag="PKO10_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014473704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HNW65750.1"
                     /translation="AIIYVELGERRVPVSYSRKVIMQNQSKRVMNYIPIKVNLSGVIP
                     AIFASAILMFPATVLQGSQNKYLVMIADYMNPSSYTFNLFMFLFVVFFAFFYASITFN
                     AKDISENLKRQGGFIPGVRPGASTAEFLNTVASRLTFWGAIYMGLISTLPWLIVKAMG
                     VPFYFGGVSVLIVVQVAIDTMRKIEAQQYSNKYQTLSAVGL"
BASE COUNT         1436 a          779 c          466 g         1636 t
ORIGIN      
        1 ttacttcaag aacatcgtca acatctacaa aagataattc catgtcgatt tgtgtaaatt
       61 ctggttgtct atctgctctt aaatcttcat ctctaaaaca ttttgcaatt tgaaaatatc
      121 tatcaaatcc agcaaccatt aaaagttgtt tgaatagttg aggagattga ggaagtgcat
      181 aaaactcacc tgcatgaact cttgatggaa ctaaataatc tcttgctcct tctggagttg
      241 attttgttaa aattggagtt tcaacatcta ggaaacctaa ttcatcaagt gtatttctag
      301 cttgaatagt agctttactt cttagttgga aaatatcaaa agatttttta cttctaagct
      361 ctaaaaatct atttcttagt tttatctcat catttacttt ctcatcattt atatcaaaag
      421 gcatagcttt gcttctattt tcaataacaa gattctctaa aataatctca atttttccag
      481 tctctaaatt tggattttct aaccctttac ctctagctcg cacagttcca gttgcaatta
      541 aaacatactc atctcttaca gtttcagcaa caactaaggc atctttacaa tcttgtggat
      601 cagcaacaag ctgaactaaa ccacttttat ctcttaaatc tataaaaata attccaccgt
      661 gatctcgtct gctattaacc caaccagcaa cagtaacttt ttgacctatt aaattctctt
      721 ttacacttgt attataatgt gttctcaaaa ttaaactcca ttttctaaat aaggggtgat
      781 tttatccaaa ttttacttaa tctcaacaaa tccatactct gtacttttaa atagtcttgg
      841 agtataaaca gcttgatttt ctactatttt agttggtatt aaattattgt ttccatttac
      901 taaatagttt gcaccaacca aagttgagta atcaacccat tcaaaattta tatttccacc
      961 aaaatttaaa atctcatctc ttaatttgtt atcaattttt tcaatagagt ttgcaagaac
     1021 cattttttct ctatctttat cttgagttag cgtcattaac ataggatcaa aatttatttg
     1081 agtagaaaat ataaactttg gataaatatc atttgctctt agttgagaca ttattagtga
     1141 actttttaca atatctacat ttaaaaatat tgagctattt cttaatcttg aatctacaac
     1201 aatattttta aagtttgttt caccactttt tatctctttt cttgctgttg tataactaac
     1261 aacattttca taactatttt ttaacttatt tcccaaatat gtattttgat aaaaaagtac
     1321 atttggacca tcagagtaat aacttaattt tttaagttga tcttcataag agatagaacc
     1381 aaaaattaaa ttatcgtttt gagataaact atcttttttt tcaattagtg gaagataaac
     1441 cataatatca tttagtgaca ttgtatttaa attatttatt gcatttggtg tataaagtgc
     1501 aattactttt gtaattccct cttctttaac tttattaaat gcattattta tactatcagc
     1561 actctcattt tgactatcaa ttacaactaa atcataattt gcatttaaaa atgtcaaata
     1621 tgcagcaact gtatttatag aattttttgc atatttagat actaaagagg atggataaac
     1681 aaaggcaatc ttaagatttg cctttgtgct atcaaatttt atttcaggtt cactagatgc
     1741 taaaacttca ctattctctt cttgtgttat ctcattttta gaaccattgt tgtttataat
     1801 ctcttcttga attacactat cgtttgagat aaactcttca acaactgttt ttttatcttc
     1861 ctcaactttt tttactggaa cattctgatt tggcattttt agctttacaa ctttttgtgt
     1921 acaacccgcc actaaaaaag ccataaaaat taaaataagt agatgcttca atttaactct
     1981 cctcatatag atttttgatt tttaacatat caagataagt atcttttttc aaagatggat
     2041 tttttagtaa ataaagagga gaaaagattg gtactaaaat agcagaatta aagcttaaaa
     2101 cttttcctct attttttaaa aattcaccac tagtttttaa caaatagtca tgaactctct
     2161 ctcctacagc aacaataatt tttggtttta taacctcaat ttgctttaac aaataacaat
     2221 tacaactctc aaaattttga aaacttgcct cttttgttcc tctacatttt actaagtttg
     2281 tagtgtaaac ttcctctttt tttattttta aactaccttc aatcattttt gctaacatat
     2341 caccagaatt tccaacaaaa aaagaaccca aatcatcttc actttttgat ggctcatcgc
     2401 aaataaacat aatttttgaa ttctgacttc catatccaaa aagtgtatgt tttcttcttt
     2461 ttgacaaatc acacaaatag cagtttgtaa ctgatttttt aagctcttct aaactatttg
     2521 gcaacgcaaa atcacaacta ctataaaagt ttatatttaa gtcactatga tatttaattc
     2581 caaaaagttt ttgattataa aggtatttta aaacttgatt cttaacttta tttgtcatac
     2641 ttcattttac aaaaaatatg ctttagtatc aatttttaag aagatttatt aaatagagaa
     2701 acttctctat ttaataacta tttttttcta tgaacaattc ttcccttatc aagtgaataa
     2761 ggtgttatct caactgttac agtatcattt ggtaatattt ttatatagtg cattctcatt
     2821 tttccagata tatgacacaa tacaatatga ccattttcca actgaactct aaacattgca
     2881 tttggtaaag cttcaactac ttttccatca acaacaatta catcatcttt tgccacatta
     2941 gccccttaat tttctctatt acttaaaata actgctcgtc catcaactac agcaaccgta
     3001 tgctcataat gactccctct taaaccatca gctgaaacaa catcccaacc atttttaaga
     3061 ataacagggt ttctatcttt ttgacatatc atgggttcta tacaaaacac cattccattt
     3121 tttatttttg gtccagattt tgtaccacca tgttctaagt agtttggaat ttcaggttct
     3181 tcatgtggtt ttcttccaat tccatgtcca caaaatctaa ctaatggctg ataacctctt
     3241 gaagtaataa aatcttcaat agcttttgat aactctttaa atctcatacc atcttcaatt
     3301 atgtcaatag cataatacaa agcatctttt gcacaagcta tcagctcttc atctttttta
     3361 gagatatctc caattggcat tgtaatagca ctatcaccat accaaccgtc aacttcagtt
     3421 cctatatcaa gacctaaaat atcaccctct tttaaaactg tatcgcttgg aattccatga
     3481 attataactt catttagtga tgtacaaaca gcatttggaa atccatataa acctttaaaa
     3541 gcaggtctag ctcctaggct taaaataaat ttttcgccca tagcatcaac ttcttttaaa
     3601 gtcattccag cttttacatt ctcttctaga tattttagtg tttttgcaac agcttgattt
     3661 gcaacaaaaa gtttttcaat ctcttcaggt tttctaagcg caatagacat tttatagtcc
     3721 aactgcactt aaagtttgat atttatttga atattgttga gcctctattt ttctcatagt
     3781 atcaatagca acttgaacta caattaaaac tgaaacccct ccaaaataaa aaggaacacc
     3841 cattgctttt acaattaacc aaggaagtgt agagattaat cccatataaa tagctcccca
     3901 gaaagttaat ctactagcaa ctgtatttaa aaactcggct gtactagctc ctggtctaac
     3961 accaggtata aatccacctt gtctttttaa gttttcactt atatcttttg cattaaatgt
     4021 aattgatgca taaaaaaatg caaagaaaac tacaaataaa aacataaata agttaaatgt
     4081 atatgaacta ggattcatat aatcagctat catcactaaa tatttatttt gacttccttg
     4141 taaaactgtt gctggaaaca tcaaaatagc acttgcaaaa atagctggga taactccact
     4201 taaatttact ttaattggaa tgtaattcat aactctttta ctttggtttt gcataattac
     4261 ttttcttgag tatgaaacag gaactcttct ctctcctaac tcaacataaa taatagc
//
LOCUS       DAOQAN010000030         4227 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_27307_length_4227_cov_2.705896, whole genome shotgun sequence.
ACCESSION   DAOQAN010000030 DAOQAN010000000
VERSION     DAOQAN010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 4227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4227
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27307_length_4227_cov_2.705896"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1030)
                     /locus_tag="PKO10_00900"
     CDS             complement(<1..1030)
                     /locus_tag="PKO10_00900"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNW65751.1"
                     /translation="MKKYRVKYQENGKIKVDIVDDDKLLFIKKSKNILEIKEINKIFN
                     IFKKEIRIDNKKLAQLFYELNLMLKSNINISDALQILIKNRKEKKIVEFLKTINYAFS
                     NSKPIKDELKRFKIDDTVKSFLQISQNSANLDLNIEAISTLLNETKDIKKSFYKAISY
                     PIFLLISFLISIFIIFLFVVPNFKTIFAQSQNNLPIATKILLAMEDFFVNHTLFIIII
                     PFLVLFFIWIFYKISKSFSSFLDKFLFKTLPISSKIYQNFEFYKLFLMIDIMQKSKYE
                     FHKAFETSRLLVKNKYLLDKIQIIDNLLQNGKTISYSFKNVDIFDDIVLNLLNTGEVS
                     NSLELTIYE"
     gene            complement(1030..2385)
                     /locus_tag="PKO10_00905"
     CDS             complement(1030..2385)
                     /locus_tag="PKO10_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HNW65752.1"
                     /translation="MSILKTKIDYTLFEKYDKEYFIENKIVPIYEDSISLKIAICPSS
                     NIDKIKDNFIKIINFIEEKEHDILFILAHIEKRVNLHKSALKSISSNDDEKFTSYFLD
                     ELILYSIEQRASDIHIEKYQDLCLFKFRVDGRLRIFFSFDEELFRVFSSFVKLISNLD
                     MTQIRLALDGRFSRNINDKKYDFRLSTMPTIQAESIVLRILDNKNIDKRLDDLGLSQN
                     IYEDLKDVLKFTQGLILISGPTGSGKTTTLYSILKELNCDDKKIITVEDPIEYKIDSI
                     NQVPINNKVGLSFELVLKNILRQDPDIIFIGEIRDKFSLDIALQASLTGHLVLASIHS
                     SSSVETITRLIDLKADPFLISTTLKAVMAQRLVLAHCKSCENGCKECNFTKFYDRTSI
                     AEILKIDEQISSLIFNKASFNELKEYLKNINFKTILDDGKQKVKNGLTTLEEVYKVVN
                     Y"
     gene            complement(2360..3073)
                     /locus_tag="PKO10_00910"
     CDS             complement(2360..3073)
                     /locus_tag="PKO10_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW65753.1"
                     /translation="MLANFKNSFENSSLKTKIELYLLPILLLFLLYVLFYNEKIEENQ
                     NVQNSELLNIENKKFTDSILELSNKIEDIAKSENLIIQKTQSSKEQIIIQLKGKRDYL
                     LNFLQKVEEINSFTKIDFLSLKKFENDIYLIDVRVDVSKYYLKNKKVKDIINIEQEDI
                     NGTKEDEYKEEIIVRPDFKINAIVGNNTFINGTWFELNDEVLGYKIETIANDYVILQN
                     NKDIIKLEVNSIEYFKNKN"
     gene            complement(3057..3950)
                     /locus_tag="PKO10_00915"
     CDS             complement(3057..3950)
                     /locus_tag="PKO10_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW65754.1"
                     /translation="MVFFANSNDIIVVDIEVTEKIDDRYLKSFVLSNLKLKNISLENC
                     DKLYVNYLEYPKEYQVFVVNSQFIFFDFEAFYSYYENRDFEGFELLIYSNFFLIFKDK
                     KFFYYQKINQDLNQDDFIKFLNKKFNINISNIKLVSKDEFEKLKKDFIQKNQKINHKK
                     NINKDGLKYIDLKSNFSFYIYIFYLLSILFIGYYFYNTYFNIVEKKEEIIDFESIKSK
                     ITFNSFEEKFYVISKNIDKNRLVLNSFDYRHDTAKIVVSSSNKENIDKFLESCENVIS
                     SSTHFNEESKIFEATIDVGKL"
     gene            complement(3989..>4227)
                     /locus_tag="PKO10_00920"
     CDS             complement(3989..>4227)
                     /locus_tag="PKO10_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HNW65755.1"
                     /translation="EKPNVDVITATIIVQKDSQKGMIIGQQATAIKRIGKAARIKIEK
                     LSGKKCFLELFVSVKKNWTKNKDALKSMGYDIDI"
BASE COUNT         1528 a          570 c          359 g         1770 t
ORIGIN      
        1 cttcataaat tgtaagttcc aaagagtttg aaacttcacc tgtatttaaa aggttcaaaa
       61 ctatatcatc aaaaatatct acgtttttaa atgagtaact aattgtttta ccattttgta
      121 ataaattatc aataatttgt attttatcca acaaatattt gttttttact aaaagccttg
      181 aagtttcaaa tgctttatga aactcatact ttgatttttg cattatatca atcattaaaa
      241 atagtttata aaattcaaaa ttttggtata ttttactact tattggtaga gttttaaaca
      301 aaaacttatc taaaaaagag ctaaaactct tgctaatttt ataaaaaatc caaataaaaa
      361 aaagaactaa aaaagggata atgattataa aaagtgtatg atttacaaaa aaatcttcca
      421 tagctaataa aatttttgta gcaatgggta ggttattttg tgattgtgca aatattgttt
      481 taaaatttgg aacaacaaat aaaaatatta tgaaaattga tattaaaaaa ctaattagta
      541 aaaatattgg gtaagaaata gctttataaa aactcttttt tatatctttt gtttcattca
      601 aaagagtaga aattgcctca atattcaaat ccaaatttgc actattttga gaaatctgta
      661 aaaatgattt aacagtatca tctattttaa acctctttaa ctcatctttt attggtttag
      721 agtttgaaaa tgcatagttt atagttttta aaaactccac aatttttttc tcttttctat
      781 tttttatcaa aatctgtaaa gcatcactta tatttatatt tgattttaac atcaaattta
      841 actcataaaa tagttgtgct aattttttat tgtcaattct aatctctttt ttgaatatat
      901 taaatatctt atttatctct tttatttcta aaatattttt ggatttttta ataaataaaa
      961 gtttatcatc atctacaata tctactttta ttttcccatt ttcctgatat ttaactctat
     1021 attttttcat caataattta ccactttata aacctcttct aaagtggtta aaccattttt
     1081 aactttttgt ttcccatcat ctaaaattgt tttaaaattt atatttttta aatactcttt
     1141 taactcatta aaactagctt tgttaaatat caaagaggat atctgctcat caatttttaa
     1201 aatttcagca atagaagttc tatcataaaa ttttgtaaag ttacactctt tacaaccatt
     1261 ttcacaactc ttacagtgag ccaaaaccaa tctttgtgcc ataactgctt taagagttgt
     1321 tgagattaaa aatgggtcag cttttaaatc tatcaatctt gtaatagttt caacagaact
     1381 tgaagagtga atacttgcaa gtactaaatg tcctgttaat gatgcttgaa gagcaatatc
     1441 aagtgaaaat ttatctctta tttcgccaat aaaaataata tctgggtctt gtcttaaaat
     1501 attttttagt accagttcaa aactaagtcc tactttgtta tttataggaa cttgatttat
     1561 actatcaatt ttgtattcaa taggatcttc aactgttatt atttttttat catcacaatt
     1621 tagctctttt aaaattgagt aaagagttgt agtttttcca cttcctgttg gtccactaat
     1681 taaaattaaa ccttgagtga attttagtac atcttttaga tcttcataaa tattttgact
     1741 aagacctaaa tcatctaatc ttttatctat atttttatta tctaatatcc ttaaaacaat
     1801 tgattctgct tgaattgttg gcattgttga aagtctaaaa tcatactttt tatcatttat
     1861 atttcttgag aatcttccat ctagtgccaa acgaatttgt gtcatatcta gatttgaaat
     1921 taattttaca aaggatgaaa aaactctaaa caactcctca tcaaaactaa aaaagattct
     1981 taatcttcca tcaactctaa atttaaacaa acacaaatct tgatactttt caatgtgaat
     2041 atcacttgct cgttgctcaa ttgagtaaag aattaactca tctaaaaaat atgatgtaaa
     2101 tttctcatca tcatttgaag agatagattt taatgcagat ttatgcaagt ttactctttt
     2161 ttcaatatga gctaaaataa aaagaatatc atgctctttt tcttctataa aatttataat
     2221 ttttataaaa ttatctttta ttttatctat atttgaacta ggacaaatag ctatttttaa
     2281 actaatacta tcttcatata ttggcacaat tttattttct ataaaatact ctttgtcgta
     2341 tttttcaaat agagtgtaat caatttttgt ttttaaaata ctcaatacta tttacctcca
     2401 attttataat atctttatta ttttgtagta tcacataatc attagcaata gtttctatct
     2461 tataacccaa aacttcatca tttaattcaa accaagtacc atttatgaag gtattattcc
     2521 ctacaattgc atttatttta aaatctggtc ttacaataat ttcttcttta tattcatctt
     2581 cttttgttcc atttatatct tcttgctcta tatttattat atctttaact tttttatttt
     2641 ttaaataata ttttgataca tcaactctta catctattaa ataaatatca ttttcaaatt
     2701 ttttcaaact caaaaaatca atttttgtaa aactatttat ctcttcaact ttttgtaaaa
     2761 aatttaataa ataatctctt tttcctttta gttgaattat aatttgctct tttgaacttt
     2821 gagttttttg aataattaaa ttttcacttt ttgcaatatc ttcaatttta tttgatagtt
     2881 ccaaaataga atctgtgaat tttttattct ctatatttag caattcacta ttttgaacat
     2941 tttgattctc ttcaattttt tcattataaa atagtacata taataaaaat aagagaagaa
     3001 taggtaataa ataaagctct atttttgttt ttaaagagct attttcaaaa ctatttttaa
     3061 agtttgccaa catcaattgt agcctcaaaa attttactct cttcattaaa atgagtagaa
     3121 gatgatatta cattttcaca actttctaaa aatttatcaa tattttcttt atttgaagag
     3181 cttacaacaa tttttgcagt atcgtgtcta taatcaaaag agttcaaaac taatctattt
     3241 ttatcaatat tttttgatat aacatagaat ttttcttcaa aactattaaa agtaatttta
     3301 gattttattg attcaaaatc tataatttcc tctttttttt caacaatatt aaaataagta
     3361 ttgtaaaaat agtatccaat aaataaaata gacaaaagat aaaatatata gatgtaaaat
     3421 gaaaaattag attttaaatc aatatattta agtccatctt tgttgatatt ttttttatga
     3481 ttaatttttt gatttttttg aataaaatct ttttttaact tttcaaattc atcttttgaa
     3541 actaatttta tgttagatat attaatatta aatttcttat ttaaaaattt tataaaatca
     3601 tcttggttta aatcctgatt tattttttga taataaaaaa atttcttatc tttgaatatt
     3661 agaaaaaaat tactataaat taaaagttca aaaccttcaa aatctctatt ttcataataa
     3721 gagtaaaatg cttcaaaatc aaaaaaaata aattgtgaat ttactacaaa tacttgatac
     3781 tcctttggat attcaagata atttacatat aatttatcac aattttctaa agatatattt
     3841 tttaatttta agtttgataa aacaaaactc tttaaatatc tatcatctat tttttcagta
     3901 acttcaatat caacaacaat aatatcatta ctattagcaa agaaaaccat attttcccct
     3961 aaaaaataga tttaaaaaca ttctaaaact aaatatctat atcatacccc atagatttta
     4021 aagcatcttt attcttagtc caattcttct ttacactaac aaataactca agaaaacact
     4081 ttttaccact aagtttttct atcttaattc ttgcagcttt tccaattctt ttaatcgcag
     4141 tagcctgctg accaataatc attccctttt gactatcttt ttgaacaatt atagtggctg
     4201 ttattacatc aacatttggt ttctctt
//
LOCUS       DAOQAN010000031         4180 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_27757_length_4180_cov_2.908364, whole genome shotgun sequence.
ACCESSION   DAOQAN010000031 DAOQAN010000000
VERSION     DAOQAN010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 4180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4180
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27757_length_4180_cov_2.908364"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1205
                     /locus_tag="PKO10_00925"
     CDS             <1..1205
                     /locus_tag="PKO10_00925"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014473315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HNW65756.1"
                     /translation="DEVIKRAVHAVEYARTFVDDVEFSLEDAGRSEIPFMKEVMDAVI
                     SAGARTINLPDTVGYRLPTELGAMVKELSEFAGNRAIISVHNHNDLGLATANTLAAVV
                     NGARQIEVTINGLGERAGNSALEEAVMAIKVRKDVFGDLYTSINTPEIYATSRLVATI
                     TGVEPQQNKAIVGKNAFAHESGIHQDGVLKHQETYEIMRPEDVGVIKDSTLILGKHSG
                     RAAFKDKITQLGFDSVSDDELNAAFERFKTLADKKKDITDDDVRMLITDEALNHDKIY
                     ELVGLQISDCSNGMPMAAVSIKYKDEIIKDAALGDGTMDAIFKTIDRITGYSGELKDY
                     KVISVSEGKDSLAKVTTRVCFENSISFVGHGLSIDTMQATANAYLGALNSYLSQKNRL
                     TKNCGHQV"
     gene            1238..1564
                     /locus_tag="PKO10_00930"
     CDS             1238..1564
                     /locus_tag="PKO10_00930"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNW65757.1"
                     /translation="MIEINSKEEILDILKNSKAVMLYFGATNCSVCQVLRPKVEMNIS
                     LNFSKMKQYYINSNQNLDITSHFNIFSSPTILVFFEGKEFKRYGRNISLDIFNSELKR
                     LYEMVF"
     gene            1567..1986
                     /locus_tag="PKO10_00935"
     CDS             1567..1986
                     /locus_tag="PKO10_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding domain-containing protein"
                     /protein_id="HNW65758.1"
                     /translation="MKRALIICFAVFLLMQFIRPQKVEYSEDETYEIVLPPDIKEIFV
                     RACYDCHSNKINYPWYSNVAPFSWVVTNHTNEGVNALNFSNWEKYTQTQKNEKLKAIY
                     RTAHSSMPLPAYTWAHIEAELSKEDREKIRDWTGVRK"
     gene            1986..>4180
                     /locus_tag="PKO10_00940"
     CDS             1986..>4180
                     /locus_tag="PKO10_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein"
                     /protein_id="HNW65759.1"
                     /translation="MREEVTALLENRNELLTITYFKIQELFDKKYGENALVLMEIGTF
                     FEVYEVNNEKEKIGKAKEIAELLNIQLTRKNKSILENSKENPIMAGVPAISFEKHLAR
                     IIAEQKYTIAIIRQKGTPPNVSRYLDVVVSPGTNFDFVVEQDDNFITSIVIDQIRGNY
                     LIGYSAIDVTTAKCYYNEVFGTNEDKFFALDEVFNYMNMHKTSEIIVTLADKNINQKE
                     IIDYLELNLKSFHLRNFRPKITYQNELFKNVFSIESLLTPIEHLDMERSPLSTESLAI
                     LIDFVIAHDSSIIQKLSNPIKLDVSRYIYIGNNALEQLNIIDTTHNLSLIRLINNTST
                     AMGKRLLKERITNPIKDSKELLRRYNLSKELYDFHSPIENELASIYDIERLTRRIKLS
                     RLHPFELNYLYDSLVSIKEIVKFMESYKFITPPCSSDEINSFLASINSTFDLSISGRF
                     MLKDVDENMITSGVNLQIDDLNRENKLLLDKLEILKNHILSFFKSDEKSFVTINRLDK
                     EGFYISITKNRYNLVKDELLKSHLIIDDKLLLFKDFSIKTQTNSVKISCALTDDISDK
                     YVHNLKKIVEINKLVFKEKLIEFEKKFSTLLSELVVFIAEVDLTVSNIKTSKKYNYTC
                     PKIVKTKDDENFLELIELRHPIIEANEDRGVYVTNDIVLGELNLVSKEYEDNIIVKNS
                     NPTNLQSNKMHGILLFGINSSGKSSLMKAIGISVILAQAGFFVPCKSMR"
BASE COUNT         1661 a          453 c          709 g         1357 t
ORIGIN      
        1 cagatgaagt tataaaaaga gctgttcatg cagtagagta tgctagaact tttgtagatg
       61 atgttgagtt ttctttagaa gatgcaggaa gaagtgaaat accttttatg aaagaagtta
      121 tggatgcagt tataagtgca ggtgctagaa caattaactt accagatact gttggatata
      181 gattaccaac agagttaggt gcaatggtta aagagttaag tgaatttgca ggtaatagag
      241 caataatatc agttcacaat cacaacgatt taggattagc aactgcaaat actttagctg
      301 ctgtagtaaa tggagctaga caaattgaag ttacaataaa tggtttagga gaacgtgctg
      361 gaaactctgc tttagaagaa gctgttatgg ctataaaggt gcgaaaagat gtatttggtg
      421 atttatatac atctattaat actcctgaaa tttatgcaac ttcgagatta gttgcaacta
      481 ttacaggtgt tgaaccacaa caaaataaag caatagttgg aaaaaatgca tttgcacatg
      541 agagtggaat tcaccaagat ggtgttttaa aacatcaaga gacttatgaa attatgagac
      601 cagaggatgt tggagttatt aaagatagta cactgatttt aggaaaacat tcaggtcgag
      661 cagcatttaa agataaaatt actcaattag ggtttgatag tgtaagtgat gatgagttaa
      721 atgcagcatt tgaaagattt aaaactttag cagataagaa aaaagatata acagatgatg
      781 atgttagaat gttaatcact gatgaggctt taaaccacga taaaatatat gaattagtag
      841 gattgcaaat aagtgattgt tctaatggga tgccaatggc tgctgtatct ataaaatata
      901 aagatgaaat tataaaagat gcagctttgg gtgatggaac tatggatgct atttttaaaa
      961 cgatagatag aatcacagga tactctggtg aattaaaaga ttataaagtt atatctgtaa
     1021 gtgaaggaaa agattcttta gcaaaagtaa caactagagt atgttttgaa aacagtatat
     1081 cttttgttgg tcatggtttg agtattgata caatgcaagc aactgcaaat gcatatttgg
     1141 gtgctttaaa ttcatatcta tctcaaaaaa atagacttac aaaaaattgt gggcatcagg
     1201 tataaaaaga taggattttt cctatctttt tttaattatg atagaaataa acagtaaaga
     1261 agaaatttta gatattttaa aaaattctaa agctgtaatg ctatattttg gagctacaaa
     1321 ctgtagtgtt tgtcaagttt taagaccaaa agtagagatg aatatttctc ttaatttttc
     1381 taaaatgaaa caatattata ttaatagtaa tcaaaattta gatattactt ctcattttaa
     1441 tattttttca agtccaacta ttttggtttt ttttgaagga aaagagttta aaagatatgg
     1501 tagaaatatt agtttagata tttttaatag tgaattaaaa agattatatg agatggtatt
     1561 ttaaaaatga aaagagcttt gataatttgt tttgcagttt ttctattgat gcagtttata
     1621 agaccccaaa aagtagaata tagtgaagat gaaacatatg agatagtttt acctcctgat
     1681 ataaaagaga tatttgtaag agcttgttat gattgccact caaataagat taattaccct
     1741 tggtattcaa atgttgcacc tttttcttgg gttgttacaa atcatacaaa tgagggggta
     1801 aatgctttga atttttcaaa ttgggaaaaa tatactcaaa cacaaaaaaa tgaaaaatta
     1861 aaagcaatct atagaacagc tcactcatca atgcctcttc cagcttatac gtgggctcac
     1921 attgaagcag aactttcaaa agaagataga gaaaaaatta gagattggac aggagtgcga
     1981 aaatagtgcg agaagaagta acagctcttt tagaaaatag aaatgaactt ctaactatca
     2041 catattttaa gatacaagag ttatttgata aaaagtatgg cgagaatgct ctagtactta
     2101 tggaaattgg aacttttttt gaagtttatg aagtaaataa tgaaaaagag aagataggaa
     2161 aagcaaaaga gatagcagaa cttttaaata ttcaattaac aagaaaaaat aaatcaatac
     2221 ttgaaaactc aaaagagaat ccaattatgg ctggagttcc agcaatttca tttgaaaaac
     2281 atctagcacg aattattgct gagcaaaaat atacaatagc aataataaga caaaaaggaa
     2341 ctcctccaaa tgtgagtaga tatttggatg ttgttgtaag tcctggtaca aactttgatt
     2401 ttgtagtaga acaagatgat aattttataa cttcaattgt aattgatcaa ataagaggaa
     2461 attatctaat aggttatagt gctattgatg taacaacagc aaagtgttat tacaacgaag
     2521 tttttggaac gaatgaagat aaattttttg cacttgatga agtctttaat tatatgaata
     2581 tgcacaaaac tagcgaaatt atagttactc tagcagataa aaatattaat caaaaagaga
     2641 taattgacta tttggagtta aatttaaaaa gttttcattt aagaaacttt agaccaaaaa
     2701 ttacatatca aaatgaactt tttaaaaatg tttttagcat tgagtcactt ctaactccaa
     2761 tagagcatct tgatatggag agatctcctc tttcaacaga atcattggca attctaatag
     2821 actttgtaat agctcatgat agttcaatta ttcaaaaact atcaaaccca ataaaattag
     2881 atgttagtag atacatatat attggaaaca atgctttaga gcagttaaat attatagata
     2941 caacgcataa tttaagtcta ataagattaa taaataatac ttcaactgct atgggtaaaa
     3001 gacttttaaa agagaggatt acaaatccaa taaaagactc aaaagagctt ttaagaagat
     3061 acaatttatc taaagaatta tatgattttc actcaccaat agagaatgaa ttagcaagta
     3121 tttacgatat agaaaggctt acaagaagaa taaagttatc aagactgcac ccttttgagt
     3181 tgaactattt gtatgactct ttggtaagta taaaagagat tgtaaaattt atggagagtt
     3241 ataaatttat aacacctcct tgtagtagtg atgagataaa ctctttttta gcatctatta
     3301 actctacttt tgatttaagt ataagtggaa gatttatgct aaaagatgta gatgaaaata
     3361 tgataacaag tggagtaaat cttcaaattg atgatttaaa tagagaaaat aaactacttt
     3421 tggataaatt agaaatttta aaaaatcata ttttatcttt ttttaaatca gatgaaaaaa
     3481 gttttgtaac aattaatagg cttgataaag aaggatttta tatctcaata acaaaaaata
     3541 gatataacct tgtaaaagat gaacttttaa aatctcatct tataattgat gataaacttc
     3601 tactttttaa agatttttct ataaaaactc aaacaaatag tgtaaaaata tcttgtgctt
     3661 taactgatga tatttcagat aaatatgttc ataatcttaa aaaaatagtt gagataaata
     3721 aacttgtatt taaagagaaa cttattgagt ttgaaaaaaa gttttcaaca cttttaagtg
     3781 aattagttgt atttatagct gaagttgatt taactgtttc aaatataaaa actagtaaaa
     3841 aatataacta cacttgccca aaaattgtaa aaaccaaaga tgatgagaat tttttagaac
     3901 taatagaact taggcatcct ataattgaag caaatgagga taggggggtt tatgttacaa
     3961 atgatattgt tttaggtgaa ttaaatttag ttagtaaaga gtatgaagat aatattatag
     4021 taaaaaactc aaatcctaca aatcttcaaa gtaataaaat gcatggaata cttctttttg
     4081 gaataaatag ctctggaaaa tcttctttaa tgaaagctat tggaataagt gttattttag
     4141 cacaagcagg tttttttgta ccttgtaaat ctatgagatt
//
LOCUS       DAOQAN010000032         4143 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_28075_length_4143_cov_2.744129, whole genome shotgun sequence.
ACCESSION   DAOQAN010000032 DAOQAN010000000
VERSION     DAOQAN010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 4143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4143
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28075_length_4143_cov_2.744129"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..172)
                     /locus_tag="PKO10_00945"
     CDS             complement(<1..172)
                     /locus_tag="PKO10_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HNW65760.1"
                     /translation="MSNEDGLLELREQLDSIDNKILDLLNDRMKLVHKVGALKAKSGG
                     AIYRPDREKAIID"
     gene            complement(183..1391)
                     /gene="lysA"
                     /locus_tag="PKO10_00950"
     CDS             complement(183..1391)
                     /gene="lysA"
                     /locus_tag="PKO10_00950"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HNW65761.1"
                     /translation="MNIDFKELANKYQTPYYVYDFDHITAQYTQLKESFRARKSLIAY
                     AVKANSNLSVIKHLANLGAGADCVSIGEVRRALKAGIPSYKIIFSGVGKSDEEIKEAL
                     NLDILLINVESAEELNRVETISKELNKIARISIRVNPNIDPKTHPYISTGLHENKFGV
                     DIDTAKRMYIQCKNSESLEPTGIHCHIGSQLTQLQPIKDAVKIVADLVRNLKAIKIEL
                     SFMDVGGGLGIVYKDETLIDTYEYTQSILDVMFGLDLTVICEPGRFIVGNSGVFVTKV
                     LYEKVNGNKRFIIVDGAMNDLIRPALYNAYHRIEVLNDNKEFSDCNLVGPVCESGDFF
                     AKNIELPKTNHNDLIAIYSAGAYGFTMASNYNTRERVAEIAVENGVDRLIRRRETFED
                     LIALEEEFIK"
     gene            complement(1496..2326)
                     /gene="fabI"
                     /locus_tag="PKO10_00955"
     CDS             complement(1496..2326)
                     /gene="fabI"
                     /locus_tag="PKO10_00955"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013134235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HNW65762.1"
                     /translation="MIMKGKKGVILGVANDKSIAYGIAKACAAQGAQIAFTYLNDALK
                     KRVEPIAAEFGSSNLVYPCDVSKPEEIVALRESLKKDLGEIDFIVHSIAFAPKEGLSG
                     RFHNISKEAFDIAMDVSVFSLIEVTRELKPLLSSNSSILTLTYYGGAKYIPNYNLMGV
                     AKAALEMTTKYLAEDLGKDGTRVNAISAGPIKTLAAAGIGDFRFMLKWNEAHSPLKKN
                     VSIDEVGNSGMYLLSDLSSAVTGEIHYVDSGFNIMGMPAVEFDEDGGKPKIAWNGTDK
                     "
     gene            complement(2323..>2933)
                     /locus_tag="PKO10_00960"
     CDS             complement(2323..>2933)
                     /locus_tag="PKO10_00960"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HNW65763.1"
                     /translation="EIYIFPTSTSLDSFELNHKLFIGAQNAYHTKNGSFTGEIGLEQL
                     EEFNIKTILIGHSERRHILGETQEEIAKKYEFYKNLGFKIIYCIGEPLEVKKAGLEKT
                     LEYIFEQFIGIDTNYENLILAYEPVWAIGTGITATNEDIKQVHNAIKSKINKPLLYGG
                     SVKVENVKEICEIENVDGALIGTASWKIEDFIKILENTKDIR"
     assembly_gap    2934..2943
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2944..3044)
                     /locus_tag="PKO10_00965"
     CDS             complement(<2944..3044)
                     /locus_tag="PKO10_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HNW65764.1"
                     /translation="MPIIASNFKTNHTRKSTSLFVEKLNEYLKSNEA"
     gene            complement(3045..>4143)
                     /locus_tag="PKO10_00970"
     CDS             complement(3045..>4143)
                     /locus_tag="PKO10_00970"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012661157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNW65765.1"
                     /translation="RRIRSALNTIRYCIDNDCSVILASHFGRPKGGFEEEFSLVPIAR
                     RLHTLLKQEIKMAPGIICDETINMAKELQSGEILLLENMRFEAGETKNDEELSKKLAS
                     MADVYINDAFGVSHRAHSSVEGIAKYFDMNHKAAGFLLAKEIKFFHHIVENPKRPFVS
                     IVGGSKVSGKLEALYNLVPKVDKIVIGGGMAFTFLKAQGYEIGKSLVEEDLIPEALKI
                     MELAKQKGVKLYLPVDIVAAEAFDAEAIAKIVPVQEIPKSWMGLDIGPATALLFSEVL
                     SDANTILWNGPMGVYEMEKFAKGSTKISHAVASSYATTVVGGGDTADLVRITGDEDDM
                     TFISTGGGASLELIEGKILPGVKALVIEDDK"
BASE COUNT         1341 a          765 c          445 g         1582 t
ORIGIN      
        1 ggtcaataat tgctttttct ctatctggcc tataaatagc tccaccactt tttgctttta
       61 aagctcctac tttatgaact aatttcattc tatcatttag aaggtctaga attttattat
      121 ctatactatc taattgttct cttagttcta ataatccatc ttcatttgac ataaaattta
      181 ccttatttta taaactcttc ttctaaagca attaaatctt caaaagtttc tcttctcctg
      241 attaatctat caactccatt ttcaacagca atttcagcaa ctctttctct tgtattgtag
      301 ttacttgcca ttgtaaaacc ataagctcca gcactataaa ttgcaattaa atcattatga
      361 ttagtttttg gtagttctat atttttcgca aaaaaatctc cactttcgca aacaggacct
      421 accagattac aatcactaaa ctctttattg tcatttaaaa cttcaattct atgataagca
      481 ttgtataaag caggtcttat taaatcattc atagcaccat caactataat aaatctttta
      541 tttccattta ctttttcata taaaactttt gttacaaaaa ctccagaatt tccaactata
      601 aatcttccag gttcacaaat aactgttaaa tcaagtccaa acataacatc taaaattgat
      661 tgagtatatt catatgtatc aattaatgtt tcatctttat atactattcc taatcctcca
      721 ccaacgtcca tgaaagatag ttcaattttt atagctttta aatttctaac taaatcagca
      781 actattttta cagcatcttt tattggttga agttgagtaa gttgagatcc aatatgacaa
      841 tgaattccag ttggttctaa agattcacta tttttacatt ggatatacat tctttttgct
      901 gtatcaatat ctactccaaa tttattttca tgaagtcctg ttgaaatata aggatgagtt
      961 tttggatcaa tatttggatt tacccttata gagattcttg caattttatt taattctttt
     1021 gaaatagttt caactctatt tagttcttca gcactctcta catttattag taaaatatca
     1081 agatttaaag cctcttttat ttcttcatca ctttttccaa cacctgaaaa tattattttg
     1141 tatgaaggaa ttcctgcttt taaggctctt cttacctctc caatactaac gcaatcagca
     1201 ccagcaccta ggtttgctaa atgttttatt acacttaaat ttgaatttgc tttaactgca
     1261 tatgcaatta aagattttct tgctctaaaa gactctttta attgtgtgta ttgagctgta
     1321 atatgatcaa aatcataaac ataatatggt gtttgatatt tatttgccag ttctttaaaa
     1381 tctatattca tataataatc ctattgttaa aattttaaat ctaaactttt caaagtttag
     1441 atttaaaatt ttgatataaa aaaagataag aaaaaaattc ttatcttttc aaaatttatt
     1501 tatcagttcc attccaagca atttttggtt ttccaccatc ttcatcaaac tcaactgctg
     1561 gcattcccat gatattaaat ccactatcaa catagtgaat ttcaccagtt actgcactac
     1621 ttaaatcact aagtaagtac attccagagt ttccaacttc atcaatagat acatttttct
     1681 ttaaaggtga gtgagcttca ttccatttta gcataaatct aaaatcacca attccagcag
     1741 ctgctaaagt tttaattggt cctgcactaa ttgcatttac tctagttcca tcttttccca
     1801 aatcttcagc taaatatttt gtagtcatct ctaaagcagc ttttgctaca cccattagat
     1861 tataatttgg aatatatttt gctccaccat aataagttag agttaaaatt gaagagttag
     1921 aagataaaag tggttttaat tcacgagtaa cttctattaa agagaataca gatacatcca
     1981 ttgctatatc aaatgcctct tttgatatat tgtgaaatct tccactaagt ccctcttttg
     2041 gagcaaatgc aattgagtga acaataaaat caatctctcc taaatctttt tttaaagact
     2101 ctcttaaagc aacgatctct tcaggtttag atacatcaca aggataaact aaattagagc
     2161 ttccaaactc agcagctatt ggctcaactc tttttttaag agcatcattt aaatatgtaa
     2221 atgcaatttg agcaccttgt gcagcacatg cttttgcaat tccataagca attgatttat
     2281 catttgcaac acctaaaata acaccttttt tacccttcat tatcatctta tatcctttgt
     2341 attttctaaa atttttataa aatcttctat tttccatgaa gctgttccaa ttaaagctcc
     2401 atcaacattt tctatttcgc aaatctcttt tacattttca acttttacac ttccaccgta
     2461 aagtaaaggt ttatttattt tagattttat tgcattatgt acttgtttta tatcttcgtt
     2521 tgttgctgta ataccagttc ctatagccca aactggttca tatgctaaaa ttagattttc
     2581 ataatttgta tcaattccaa taaattgttc aaaaatatac tctaaagtct tttctaaacc
     2641 agcttttttt acttctaaag gttctccaat acaataaata attttaaatc ctaaattttt
     2701 ataaaactca tatttttttg caatctcttc ttgagtttca cctaaaatat gtcttctttc
     2761 actatgacct attaaaatag tttttatatt aaactcttca agttgctcta agccaatttc
     2821 accagtaaaa gatccatttt tagtatgata tgcattttgt gcaccaataa aaagcttatg
     2881 atttaattca aatgaatcta aagatgtcga agtagggaag atgtaaattt catnnnnnnn
     2941 nnnaaagctt catttgattt taaatattca tttaattttt caacaaataa acttgtactt
     3001 tttctagtat gatttgtttt aaaattactt gcaattatag gcatttattt atcatcctca
     3061 ataactaatg ctttaactcc aggtaatatt tttccctcta ttaactctaa agaagctcca
     3121 ccaccagtag atataaatgt catatcatct tcatcaccag taattcttac taaatcagct
     3181 gtatctcctc caccaacaac agttgttgca tatgagcttg ctactgcatg agatattttc
     3241 gtacttcctt ttgcaaattt ttccatttca taaacaccca ttggtccatt ccataaaata
     3301 gtatttgcat cgcttaaaac ttcactaaat aaaagagcag ttgcaggtcc aatatcaagc
     3361 cccatccaag attttggtat ctcttgaact ggaacaattt ttgctattgc ttcagcatca
     3421 aaagcttcag ctgcaacaat atcaactggt aaataaagtt ttacaccttt ttgttttgct
     3481 aattccataa tttttaaagc ttcaggaatt aaatcttctt caaccaaaga ttttccaatt
     3541 tcataacctt gtgcttttag gaatgtaaat gccattcctc caccaattac aattttatca
     3601 acttttggaa caagattata aagggcttca agttttccag atacttttga accaccaact
     3661 attgaaacaa atggacgttt aggattttca acaatatggt ggaaaaattt aatctctttt
     3721 gctagtaaaa atccagcagc tttatgattc atatcaaaat attttgcaat tccttcaaca
     3781 gaagagtgag ctctgtgaga aactccaaat gcatcattta tataaacatc tgccatggaa
     3841 gctaattttt tactaagctc ttcatcattt tttgtctcac cagcttcaaa tctcatattt
     3901 tctaaaagta atatttcacc tgattgaagc tcttttgcca tattaatagt ttcatcacaa
     3961 ataatacctg gagccatttt tatctcttgt tttaaaagag tgtgtaatct tcttgcaatt
     4021 ggaactagtg aaaactcttc ttcaaatcca ccctttggtc ttccaaaatg agatgctaat
     4081 ataacagagc aatcattatc aatacaatat ctaattgtgt ttaaagcact tctaattctt
     4141 cta
//
LOCUS       DAOQAN010000033         4030 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_29260_length_4030_cov_3.095094, whole genome shotgun sequence.
ACCESSION   DAOQAN010000033 DAOQAN010000000
VERSION     DAOQAN010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 4030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4030
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29260_length_4030_cov_3.095094"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2910)
                     /gene="mfd"
                     /locus_tag="PKO10_00975"
     CDS             complement(<1..2910)
                     /gene="mfd"
                     /locus_tag="PKO10_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HNW65766.1"
                     /translation="MKNIYEFLKNLKDEKSLKKCQLLIVNDDSQAQIASNIVSFLGFK
                     PFVLADFRANFKDDLLSFSDELQDITKSLQDFYSYKKEDKILISPIRTISFPMPKKEC
                     FAELKISFADNLDLSEFKDKLYSWGYYFVDIVTSSGEVSLRGDIFDIAPLGSDFGYRV
                     SLFDNEVESIRKFDIEDQKSQKEELDSFTITPAFLALSQDNFEELSSKIELSSSDAFV
                     KDIHSLGFWYLDDLAHYLPQNMDSFITQEALNELDEAYIFEEKRLNKDKFLTIPQIYS
                     SKEYKEISPSNIKEFISFHKDKKITIITTSEARVKALDLPLDDKAIKYYFGDEIINLL
                     GSNELIISLNKEIKKKRKKRVKLVVDELVLNDFVVHEKHGIGIYKGIEPVTIMGAKRD
                     FVVINYQGEDRLLIPVENIDTIDRYVADGESYAIVDKLGRGSFAKLKEKVKDRLFEIA
                     NDIIRLAAARELVNGIKIDIDDYLIKDFQLSAGFKYTKDQTRSINEILADLSSGKVMD
                     RLLSGDVGFGKTEVAMNALLAVINSGYQTVFVCPTTLLATQHYHSISKRFKEFGIDVY
                     KLDGKTSTKEKNIIKSGLEDGSIKLVIGTHSLLDVKTSNLALVIIDEEHKFGVKQKEK
                     LKALREDVHIFSMSATPIPRTLNLALSKLKGMSTLLTPPSERLGVRTFVKEYSDALIK
                     EVILREKRRGGQLFYVHNNIASIEAKKKDLLQIVPNIKIEIIHSQIKADATEKIIEAF
                     ENKEFDILLATSIVESGIHLPNANSIIIDGADRFGIADLHQLRGRVGRSNKEGFCYYV
                     VEDKSKITPEAIKRLVALESNSYLGSGTALAHQDLEIRGGGNIIGAEQSGHIKQIGYG
                     LYLKMLEDTLAVLSGETKTSEQNIDIKLAISAYISEDYISEDRIRLELYRRLSKAVSK
                     ETLYEIEEEMEDRFGKLDIPTKQFLELILIKILAISKGINQISSYEMNITFVKADG"
     gene            complement(2913..3215)
                     /locus_tag="PKO10_00980"
     CDS             complement(2913..3215)
                     /locus_tag="PKO10_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013134477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW65767.1"
                     /translation="MITIDVLVLLDVVGLEDRDKFEKHVKKEGFIKVENEDFVYTGNS
                     TTTTFATKAYILEVFKKGLQKSGFETASLVFLLNETPYPPYIYDKNTNDFELSEVK"
     gene            3336..3746
                     /locus_tag="PKO10_00985"
     CDS             3336..3746
                     /locus_tag="PKO10_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW65768.1"
                     /translation="MNYELLSRLAMQDSERLKSENLILVKKIVLLEQENKNLKDFNNI
                     IEKERNAYKERMGQIKNEANGLIIEKNLLLDEIIILENKIKKLEEKLLAKEEERQETN
                     NLRQKIRIDEDGEIIKNKDQIRAKNISKSVEFYS"
     gene            complement(3788..>4030)
                     /locus_tag="PKO10_00990"
     CDS             complement(3788..>4030)
                     /locus_tag="PKO10_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW65769.1"
                     /translation="VLDDDEKFVKDKLIVVCNTASRAAIEAQAFKQMGFKTVKIYEIN
                     KWIDECNPVITKYSAGEHKDGTKTKFGNYYAEHCKK"
BASE COUNT         1368 a          684 c          445 g         1533 t
ORIGIN      
        1 tccatcagct tttacaaaag ttatattcat ctcataagag cttatttgat ttattccttt
       61 agaaatagct aaaattttta ttaaaattaa ctctaaaaac tgttttgttg gaatatctag
      121 ttttccaaat ctatcttcca tctcttcttc aatctcatat aaagtctctt tactcacagc
      181 tttacttaac cttctataaa gttcaagtct tattctatct tcacttatat aatcttcact
      241 aatatatgca gaaattgcta gttttatatc aatattttgt tcagaggttt ttgtttctcc
      301 acttaaaact gccaaagtat cttcaagcat tttaagatac agaccataac caatctgttt
      361 tatatgtcca ctttgctcag caccaataat atttccacca cctcttattt ccaaatcttg
      421 atgagcaagt gctgttccac ttcctaaata tgagttagat tccaaagcaa ctaatctttt
      481 tatagcttca ggtgttattt tgctcttatc ttcaactaca tagtaacaaa atccctcttt
      541 gttacttcgt ccaactcttc ctcttagttg gtgtaaatca gcaattccaa acctatctgc
      601 tccatcaatt attattgagt ttgcatttgg taggtgaatt ccactttcta caatagaggt
      661 tgcaagtaaa atatcaaact ctttgttttc aaaagcttca attatttttt ctgtagcatc
      721 tgcttttatt tgagagtgaa taatctctat ttttatattt gggacaattt ggagtaaatc
      781 ttttttctta gcttcaatag aggctatgtt attgtgaaca taaaaaagtt gtccacctct
      841 tcttttttct cttaatataa cctctttgat taaagcatca ctatactctt ttacaaaagt
      901 tctcacccct aatctctcac ttggaggtgt aagaagagta ctcattcctt ttagttttga
      961 tagtgcaaga tttaaagttc ttggaattgg tgttgcgctc atagaaaaaa tatgtacatc
     1021 ttctcttaaa gcttttagtt tttctttttg ttttacacca aatttatgct cttcatcaat
     1081 tatcacaagt gcaagattag aagtttttac atcaagcaaa gagtgcgttc caattaccag
     1141 ttttatagag ccatcttcta atccactttt tataatattt ttctcttttg tactagtttt
     1201 tccatctagc ttataaacat ctattccaaa ctctttaaat ctttttgaaa ttgagtgata
     1261 gtgttgcgtt gccaaaagtg tagttggaca tacaaaaact gtttgatatc cactatttat
     1321 aacagcaagt aaggcattca tagcaacctc agttttccca aaaccaacat caccacttaa
     1381 aagcctatcc ataacttttc cactacttaa atcagctaaa atctcattta tacttctagt
     1441 ttgatccttt gtatatttaa acccagcaga taattgaaaa tcttttatta aataatcatc
     1501 tatatctatt ttaataccat ttacaagttc acgtgctgct gccagtctta taatatcatt
     1561 tgctatttca aaaagtctat cttttacctt ctcttttaat tttgcaaaac ttccacgacc
     1621 aagtttatca actattgcat aactttcacc atctgcaaca tatctatcaa tagtatcaat
     1681 attttcaaca ggaattaaaa gtctatcttc accttgataa tttattacta caaaatctct
     1741 ttttgcaccc atgatagtaa ctggttcaat tcctttgtaa attccaattc catgtttttc
     1801 atgaactaca aaatcattaa gaaccaactc atcaacaact agttttactc ttttttttct
     1861 ctttttcttt atctctttat ttaaagagat aattaattca ttgctaccta aaagatttat
     1921 tatctcatca ccaaaataat actttattgc tttatcatca agtggtaaat caagagcttt
     1981 tactcttgct tcacttgtag ttataatcgt aattttttta tctttatgaa aagagataaa
     2041 ctcttttata tttgatgggc ttatctcttt atactcttta cttgaatata tttgaggaat
     2101 agttaaaaac ttatctttat ttagcctttt ttcttcaaaa atatatgctt catctagttc
     2161 atttagagct tcttgtgtaa taaatgaatc catattttgt ggtaaatagt gtgctaaatc
     2221 atctaaatac caaaatccca aagagtgaat atcttttaca aaagcatcac ttgaagagag
     2281 ttctatttta gagcttaact cttcaaaatt atcttgactt aaagctaaaa atgcaggagt
     2341 tattgtaaaa ctatcaagct cctctttttg tgatttttga tcttctatat caaattttct
     2401 aatactttct acttcattgt caaacaaact tactctatat ccaaaatcac ttccaagtgg
     2461 tgcaatatca aaaatatctc ctcttaaaga gacttctcca gagcttgtaa caatatctac
     2521 aaaatagtat ccccaactat aaagtttgtc tttaaactct gataaatcaa gattatcagc
     2581 aaaactaatt tttaactcag caaaacactc ttttttgggc attggaaaag atattgttct
     2641 aataggagaa ataagtattt tatcctcttt tttatatgag tagaaatctt gtaaagattt
     2701 tgtaatatct tgtaactcat cgctaaaaga gagtaaatca tctttaaaat ttgctctaaa
     2761 atcagctaaa acaaatggtt tgaaacctaa aaaagagact atatttgaag ctatttgagc
     2821 ttgagaatca tcatttacta ttagaagctg acactttttt aaactttttt catcttttag
     2881 attttttaaa aattcatata tatttttcac tactacttaa cttcacttag ttcaaaatca
     2941 tttgtatttt tatcataaat atatggtgga tatggtgttt catttaataa aaaaaccaaa
     3001 gaagcagttt caaaaccact tttttgtaaa ccttttttaa aaacttctaa aatataagct
     3061 tttgtagcaa aagttgtagt tgtagagttt cctgtataga caaaatcttc attttctact
     3121 tttataaaac cctctttttt tacatgcttt tcaaacttat ctctatcttc taaaccaaca
     3181 acatctaaca aaactaaaac atcaattgta atcatcataa accttgaaaa aaaattgcaa
     3241 gattttagca taatatagtt ttatgctaaa ttacctattt ttttgatatg attatgtcaa
     3301 acaatataat attaatcttt attaaaggat ttttgatgaa ctatgaactt ctaagccgtc
     3361 ttgctatgca agattcagaa aggctaaaaa gtgaaaatct tattttagta aaaaagattg
     3421 ttcttcttga acaagagaac aaaaatttaa aagattttaa caatattatt gaaaaagaga
     3481 gaaatgccta taaagagcgt atggggcaaa tcaaaaatga agcaaatggc ttgattatag
     3541 aaaaaaatct tcttttagat gagattatca tacttgaaaa taaaataaaa aaactagaag
     3601 aaaaattatt agcaaaagaa gaagagagac aagaaacaaa caatctaaga caaaaaataa
     3661 gaattgatga agatggagaa attataaaaa acaaagatca aataagagct aaaaacatat
     3721 caaaatctgt tgaattttat tcttaacttc tctatagatg aagtagagat ttattctact
     3781 tctttatcta ttttttgcaa tgttctgcat aatagttacc aaattttgtt ttagttccat
     3841 ctttgtgttc accagcacta tattttgtaa tcacaggatt acactcatca atccacttat
     3901 ttatctcata aatttttaca gttttaaatc ccatttgttt aaaagcttgt gcttcaatag
     3961 cagctcttga ggcagtatta caaactacaa ttaatttatc ttttacaaac ttttcatcat
     4021 catccaaaac
//
LOCUS       DAOQAN010000034         4007 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_29511_length_4007_cov_3.158907, whole genome shotgun sequence.
ACCESSION   DAOQAN010000034 DAOQAN010000000
VERSION     DAOQAN010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 4007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4007
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29511_length_4007_cov_3.158907"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="PKO10_00995"
     CDS             complement(<1..395)
                     /locus_tag="PKO10_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HNW65770.1"
                     /translation="MKIFIIISLLICSLFSQTIKDISNVIGIRDNQLIGYGLIVGLPG
                     TGDKSKFTMQSLQNLLTNSYIKIPAGSINSKNIAAVMVTADLPAFSRQGDKIKVTVST
                     IGDAKSIDFGELLMTQLKGVDGNVYAVAQG"
     gene            495..1844
                     /locus_tag="PKO10_01000"
     CDS             495..1844
                     /locus_tag="PKO10_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW65771.1"
                     /translation="MNIFIYGNQNFKNEVHKILLNSKIETILEDVKIEDISNIDTLKD
                     NIAKNPDDIYLIDEDKIIKKSRFKFLKQKDGIEEDFLLQYGVNDLSIDSLEEIPNYII
                     RKYERIAFKEKDKSEEIKNEDLLLDNELANLLESKEQENSQNLTSSILEEPVGININE
                     LDNLIELESSDKEKLDTNYISMEDFDENFGLNNVSFDYDDDSTFNQDLKSDEDLLQDI
                     LNNSIVDEDDYEFVGETFEDVNFLDEIFPNSTIFDMDENSTKSINIVLQEKDKEETNE
                     DELKKDELENIENFQFNEVFQNIENKKDEDIKQIDKNIDIIENIEEKNEEEKTPEYEN
                     IEIDELNDLNLEDFSFADLDLQNKKSEDVSKKGEDMSDDFFELDSLNEQDLIEALSGS
                     GISNVAQTDTQSRQEPQKMEISNNNIDIGSSNINDIASLISKLLNNKTLEITIKIKE"
     gene            1846..2106
                     /locus_tag="PKO10_01005"
     CDS             1846..2106
                     /locus_tag="PKO10_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliQ"
                     /protein_id="HNW65772.1"
                     /translation="MDLLAIAQNTVKIILLVGMPSLMISMIIGLIISIFSAVTQVNDA
                     ALSFVPKMIFVSAFILFTLPWVGEHIETFTIELWNMILIFGK"
     gene            2107..2517
                     /locus_tag="PKO10_01010"
     CDS             2107..2517
                     /locus_tag="PKO10_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW65773.1"
                     /translation="MIQKLYNLKKTQTEQKLIEKSSLEQEVYKIDEEVGDLNHRINTA
                     TVEKLGSISDFMILAMHKDSLRFEVKKLLSKKNQLLKKIDDIFVEIIELQKESEQYKY
                     ILDEEREQKRKDALHFEILESEEFIQSKYIKGKS"
     gene            2514..3005
                     /locus_tag="PKO10_01015"
     CDS             2514..3005
                     /locus_tag="PKO10_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW65774.1"
                     /translation="MINKVLIVIVFSLSLNAAVETSSSLTKQKMEVLELKNELNNFYN
                     EKEKEYQRQKKELEDILTKIEQEKAEIKRLYEKNDEILKEIRSEVVKKSIRVFELMKA
                     KVAAEIFDQMILEGKIDDVFDIIVRLKEANVSNIMKTMSLENSSRLIQKLERYNKDKQ
                     KRD"
     gene            3008..3538
                     /locus_tag="PKO10_01020"
     CDS             3008..3538
                     /locus_tag="PKO10_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HNW65775.1"
                     /translation="MADENEVVKKTSDGKGLLIVLLVLVIVLIMAVAGGTYFLLSKIS
                     NSNQGSTNNTEEVAKASSSSASGSEAKFKADVNELVLNLTDSKGREKIMKLSFSIRSS
                     DPLIATIVQDYLAEITDVVITQISSRSSEELLTVGGKNLLKDELISEINNVINFVTKS
                     NSNVVSNILFTTFVIK"
     gene            3545..>4007
                     /locus_tag="PKO10_01025"
     CDS             3545..>4007
                     /locus_tag="PKO10_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW65776.1"
                     /translation="MIVKVRKKSSSSKILKLIIIAGLFFGIVYISLLIKEENLLSIEL
                     EKAREDEKIAIQIEQEKKEKEKLDAQRIILIEVEKVVDLIGQNNINDIKIVKNKVVYI
                     LNPNTNIDAINIRYGAMALIKKSFKEIVVVVDLEHILKGELGAKRYLYSDLS"
BASE COUNT         1741 a          359 c          660 g         1247 t
ORIGIN      
        1 ccttgagcaa cagcataaac atttccatca acacctttta gttgagtcat taaaagctca
       61 ccaaaatcta ttgattttgc atcaccaata gtagaaactg taacttttat tttatcgcct
      121 tgtcttgaaa atgctggtaa atcagcagtt accataacag cagcaatatt ttttgaatta
      181 atagaaccag ctggaatttt tatatatgag tttgtaagta agttttgtaa gctttgcata
      241 gtaaacttag atttatcacc agttcctggt aatccaacaa taagaccata accaataagt
      301 tgattgtctc ttattccaat aacatttgaa atatctttta tagtttgaga aaacaaagag
      361 caaataagta aagagatgat aataaaaatt ttcaaaaaaa tccttcataa atttttagtt
      421 tttattttat cgaaatttta ataaaaaaag gtataatcaa agcaaaattt caaactataa
      481 tagggcaaaa gatattgaat attttcatct acggaaatca aaactttaaa aatgaagtac
      541 acaaaatttt attaaattct aaaatagaga caattttaga agatgtaaaa attgaagata
      601 tatcaaatat tgatacctta aaagataata tagctaaaaa cccagacgat atttatttga
      661 ttgatgaaga taaaataata aaaaaaagca gatttaagtt tttaaaacaa aaagatggaa
      721 tagaagagga ttttttactt cagtatggtg ttaatgattt atcaatagat tcattagaag
      781 agataccaaa ttatataatt agaaaatatg aaagaattgc tttcaaagaa aaagataaaa
      841 gtgaagagat aaaaaatgaa gatttacttt tagataatga attagcaaat cttttagaaa
      901 gtaaagaaca agaaaatagt cagaatttga ctagctcaat tttagaagaa ccagttggaa
      961 tcaatataaa tgaacttgat aatttgattg aacttgagag ttcagataaa gaaaaattag
     1021 atacaaatta tattagtatg gaagattttg atgaaaattt tggtttaaac aatgtctctt
     1081 ttgattatga tgatgattca acatttaatc aagatttaaa gagtgatgaa gatttactac
     1141 aagatatttt aaataatagt atagtcgatg aagatgatta tgagtttgtt ggtgagactt
     1201 ttgaagatgt aaactttttg gatgaaattt ttccaaatag tacaattttt gatatggacg
     1261 aaaatagtac taaatccata aacatagttt tgcaagaaaa agataaagaa gagacgaatg
     1321 aagatgaact gaaaaaagat gaattagaaa acattgaaaa cttccaattt aatgaagttt
     1381 ttcaaaatat agaaaataaa aaagatgaag atattaaaca aatagataaa aatatagata
     1441 taattgaaaa tattgaagaa aaaaatgaag aagaaaaaac accagaatat gaaaatattg
     1501 aaatagatga actaaatgat ttaaatttag aagattttag ttttgcagac ctagacctac
     1561 aaaataaaaa atcagaagat gtaagcaaaa aaggagaaga tatgagtgat gatttttttg
     1621 aattagatag tttaaatgag caagatttaa ttgaagcttt aagcggttca ggtatatcaa
     1681 atgttgcaca aactgataca caaagtagac aagagccaca aaaaatggaa atttcaaata
     1741 acaatattga tataggaagt tcaaatataa atgatatagc tagtttaata tcaaaattgt
     1801 taaataataa aactttagaa attacaataa aaataaaaga gtaatatgga tctgttggca
     1861 attgctcaaa atacagttaa aataattttg ctagttggta tgccatcatt gatgattagt
     1921 atgattattg gacttataat atcaatattt tctgctgtta cacaagtaaa tgatgcagca
     1981 ctttcgtttg taccaaaaat gatttttgta tcagcattta ttttatttac acttccttgg
     2041 gttggtgaac atattgaaac atttacaatt gagttatgga atatgatttt aatatttggt
     2101 aagtagatga tacaaaaatt atataatctt aaaaaaacac aaactgaaca aaaactaata
     2161 gaaaaatcaa gtttagagca agaagtttat aagatagatg aagaagttgg agatttaaat
     2221 catagaataa acacagcaac tgttgaaaag ttgggttcaa tttcagattt tatgatttta
     2281 gctatgcaca aagatagttt aagatttgag gtaaaaaaat tattaagtaa aaagaatcaa
     2341 ctcttaaaaa aaatagatga tatatttgta gagattatag agttgcaaaa agagagtgaa
     2401 caatataaat atattttgga tgaagagaga gaacaaaaaa gaaaagatgc tttgcatttt
     2461 gaaattctag agagtgaaga gtttattcaa agtaaatata ttaaaggaaa aagttgataa
     2521 ataaagtttt aatagtaata gttttttcat tatctctaaa tgcggctgtt gaaacaagta
     2581 gctcattaac taagcagaaa atggaagttc tagagttaaa aaatgagtta aataattttt
     2641 ataatgaaaa agagaaagag tatcaaagac aaaaaaaaga gcttgaagat atcttaacaa
     2701 aaatagagca agaaaaagca gaaattaaaa gattatatga aaaaaatgat gagatattaa
     2761 aagagataag gtcagaggtt gttaaaaaat caataagagt ttttgagctt atgaaagcaa
     2821 aagttgctgc ggagattttc gatcaaatga tactagaagg taaaattgat gatgtttttg
     2881 atataatcgt aagattaaag gaagcaaatg tatcaaatat tatgaaaaca atgagtcttg
     2941 aaaattcatc aagattgatt caaaaattag aaagatacaa taaagataag caaaagaggg
     3001 actaaaaatg gcagatgaaa acgaagtggt aaaaaaaaca tcagatggaa aaggcttact
     3061 aatagtttta ttggtgcttg taattgtttt aattatggca gttgcaggtg ggacatattt
     3121 tttattgagt aaaatttcaa atagtaatca aggttcaact aacaatacag aagaggttgc
     3181 taaagcctct tctagttcag catctggtag tgaagcaaag tttaaagcag atgtaaatga
     3241 attagtttta aatctaacag attcaaaggg tagagagaag attatgaaat tatctttttc
     3301 tataagaagt agtgaccctc ttattgcaac aatagttcaa gattatttag cagaaattac
     3361 agatgttgtt attactcaaa taagttcaag aagctcagaa gagttactaa ctgttggtgg
     3421 aaagaatcta ttaaaagatg agttaatatc agaaataaat aatgtaatta attttgttac
     3481 aaagagtaat tctaacgttg ttagtaatat tctttttaca acttttgtaa taaaataaga
     3541 gaaaatgata gtaaaagtta gaaaaaagag tagcagctca aagattttaa aattaattat
     3601 tatagctgga ctttttttcg gaatagtata tatttctctt ttaataaaag aggaaaacct
     3661 tttatcaatt gagcttgaaa aagcaaggga agatgaaaaa atagctattc aaattgagca
     3721 ggaaaaaaaa gaaaaagaga aattagatgc tcagagaatt attttgattg aagttgagaa
     3781 agttgttgat ttaattggac aaaataatat aaatgatata aaaattgtta agaataaagt
     3841 tgtttatatt ttaaatccaa atacaaatat agatgctata aatattagat atggagctat
     3901 ggcacttata aaaaaaagtt tcaaagagat tgtagtagta gttgatttgg aacatatttt
     3961 aaaaggtgaa ttaggagcaa aaagatactt gtacagtgat ttgagct
//
LOCUS       DAOQAN010000035         3951 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_30148_length_3951_cov_3.963296, whole genome shotgun sequence.
ACCESSION   DAOQAN010000035 DAOQAN010000000
VERSION     DAOQAN010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 3951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3951
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30148_length_3951_cov_3.963296"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            55..1263
                     /locus_tag="PKO10_01030"
     CDS             55..1263
                     /locus_tag="PKO10_01030"
                     /inference="COORDINATES: protein motif:HMM:NF039819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HNW65777.1"
                     /translation="MANESNYSLKEVAFIKETTPGVFQTTPKLYKLGVMGFALAPTQK
                     TEQNTELGQDGQATAMDTGSLDIAGNLNMKSKTGLMPILLHSVVGKSTKVDATTDDWT
                     AATVYIGPADKFSAGQMVKHSDGTHTLVVKSVAGTGTSGATEPDLTAINPATGQVYKE
                     YDTIVDNPGANQITWIVRKKLYKHTGTSDIDMQSLGVYAKSNTAQGGGVNFEQYFTGI
                     FLNSISFAKASGTIVYKQDVPTVGLNYRDNTQLDWQPLAPSGTVEIEDKSFSFNDMKV
                     TIAGVEPEKASEFNLTLNRNVTVEDEVKQGSKDYNVPVMTMDGNLKLKFTKEKWAEVY
                     DNPTKELVITYANRSGDKISLTFPNVKTMLGTKETTTDKYDYITVPLSASGSASQKTL
                     SYETITSTDW"
     gene            1337..1936
                     /locus_tag="PKO10_01035"
     CDS             1337..1936
                     /locus_tag="PKO10_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW65778.1"
                     /translation="MEREQVFQNIIRQIGNGHQEDYENLIDEIDRREEKKLNEEYQSK
                     IDSLGDNPIDEPEIAKIIEESTKGERSQFDIAAHILGKDFIKATDMEQIALLIAKAIE
                     KVISYKDKDGVLKKTKSFESYEPMKPVDRMNYENLILKAKKTNNEKDLKDLEAKRDKF
                     IAEKTNIDVENLTSWEKNLLQEILAQEAKSISMIFLGKK"
     gene            1858..2301
                     /locus_tag="PKO10_01040"
     CDS             1858..2301
                     /locus_tag="PKO10_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW65779.1"
                     /translation="MGKKSIARNISTRSKKHFYDFFGKEIISLIDWEFDFKEERREAF
                     AWEETIEDILDSENESWIKAVIPPKATQVAYRVFNFLRTNRDEAEMLASGMRSFVKGF
                     DKLYLYQLADTQGIIDMRRYDFAIEYYKNKILKEQAESFQKEDNS"
     gene            2321..>3951
                     /locus_tag="PKO10_01045"
     CDS             2321..>3951
                     /locus_tag="PKO10_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW65780.1"
                     /translation="MASKAGITIAIKGEIDDLEAKISQSKEIINDATKSIISENNKLI
                     ASQEALNRIQKTYSKEIDNANNEIKKYLELKSQNGKLTKEEQQNLAAARAELALYKTK
                     TKELTDVERERQKEIKRNIAITRDMQNLQRASINDARTEIETKRKVIELEKQSIASKN
                     NLEKSTSNLANTTIRYLRWAGTIAGAVYAGKRAWDATIGSGIAVNKIIENNTYGIAAL
                     ISANTQMVDSLGNTLTPLQKFVMGQEDAKKVLAELRKESVKTAATFPQLIEIFQQGIG
                     KTLSMGDSFGATTEEIEKNTIKLSARMSNFANAIGMPMDRVKEEMRSLVSGNASTDSL
                     ISTIIFGSPGEANSAIREAEKKVNGVAELLDKKFKPFDILADTKTFDKSILSIQDAWS
                     RAMGDMVDKSGMFRDITNLFYGMSEQIISNTDGIIKSFDSLYNSVKNIVSIFGDIAIA
                     GAIFFIGSKLDKGLVSAARLVAGFGAAAGGATGLMLQLTLGVNALGLALKGLAMANPF
                     TALAVAGTGLFTVFSAINDEIRKMQLNGKTVEEYE"
BASE COUNT         1678 a          517 c          719 g         1037 t
ORIGIN      
        1 tggaatgtat catttaatcg agcttgaatt agtatcatat aaggagatat aagtatggca
       61 aatgaatcaa attatagttt aaaagaggtt gctttcatta aagaaactac accaggagtt
      121 tttcaaacaa ctccaaaact atacaaatta ggagttatgg gttttgcatt agcaccaaca
      181 caaaaaacag agcaaaacac agagctaggt caagatggac aagcaacagc aatggacaca
      241 ggttcattag atattgctgg aaacttaaat atgaaatcaa aaacaggatt gatgcctatt
      301 ttgcttcatt cagtagttgg aaaatcaaca aaagttgatg ctacaacaga tgattggaca
      361 gctgcaacag tttatattgg accagcagac aagttttcag caggacagat ggtaaaacat
      421 tcagatggaa cacatacttt agttgtaaaa tctgtagctg gaacaggaac atcaggtgca
      481 acagaaccag atttaacagc aatcaatcca gcaacaggtc aagtatacaa agaatatgac
      541 acaatcgttg ataatcctgg ggctaatcaa ataacatgga ttgttagaaa aaaactatat
      601 aaacacacag gtacttctga tattgatatg caatctttag gtgtatatgc aaaatcaaac
      661 acagcacaag gtggtggtgt taattttgaa caatatttta ctggtatatt tttaaactca
      721 atctcttttg caaaagcatc aggaactatt gtttataaac aagatgttcc aactgtagga
      781 ttaaactata gagataatac tcaattagat tggcaaccat tagcaccatc tggaacagtt
      841 gaaattgaag acaaatcatt ttcatttaac gatatgaaag ttactatagc tggtgtagag
      901 ccagagaaag cttctgaatt taacttaaca ttaaatagaa atgttacagt agaagatgaa
      961 gtaaagcaag gctcaaaaga ctacaatgtt ccagttatga ctatggatgg aaatttaaag
     1021 cttaaattta caaaagaaaa atgggcagaa gtatatgata atccaacaaa agaattggta
     1081 atcacttatg caaatagatc aggtgataaa ataagtttaa ctttcccaaa tgtaaaaact
     1141 atgctaggaa caaaagaaac tacaactgat aagtatgact atattacagt tccactttca
     1201 gcatctggtt cagcaagtca aaaaacatta agctacgaaa caatcacatc aactgattgg
     1261 tagtaaaaaa ggggtttatc cgatttatcc ccttgctttg cagaaaaaat tggaaaataa
     1321 aaaaacggag aaaaaaatgg aaagagaaca agtatttcaa aatataatta gacaaatcgg
     1381 aaatggacat caagaggatt atgaaaattt aattgatgaa attgatagaa gagaagaaaa
     1441 aaaattaaat gaagaatatc aatcaaaaat tgattcttta ggtgacaatc caatagatga
     1501 gccagagata gcaaaaatta ttgaagaatc aacaaaaggc gaaaggtcgc aatttgatat
     1561 tgcagcacat atcttaggaa aagattttat aaaagcaaca gatatggagc agatagcttt
     1621 acttattgca aaagcaatag aaaaagtaat aagctataaa gacaaagatg gagttcttaa
     1681 gaaaacaaaa tcttttgaga gctatgagcc aatgaaacca gttgatagaa tgaactatga
     1741 aaacttaatc ttaaaagcta aaaaaacaaa caatgaaaaa gatttaaaag acttggaagc
     1801 aaaaagagat aagtttatcg ctgaaaaaac aaacatagat gttgaaaatt taacatcttg
     1861 ggaaaaaaat ctattgcaag aaatattagc acaagaagca aaaagcattt ctatgatttt
     1921 tttgggaaag aaataatctc cctaattgac tgggagtttg actttaaaga ggagaggaga
     1981 gaagctttcg cttgggaaga aactatcgaa gatatattag atagcgaaaa tgaatcttgg
     2041 ataaaagcag ttattcctcc caaagctaca caagtagcat atagagtctt taatttttta
     2101 agaacaaata gagatgaagc tgaaatgtta gcatctggaa tgagaagctt tgtaaaaggt
     2161 tttgataagt tatatttata tcagttagcc gatacacaag ggattataga catgagaaga
     2221 tatgattttg caatagagta ttataaaaat aaaattttaa aagaacaggc ggagagtttt
     2281 caaaaagaag ataactctta gccttcttat ggagtaaaaa atggcatcaa aagcaggaat
     2341 aacaattgca ataaaaggcg aaattgatga tttagaagca aaaatatctc aaagcaaaga
     2401 aataataaac gatgcaacaa aatcaataat atctgaaaat aacaaactta tagcttcaca
     2461 agaagcacta aatagaatcc aaaaaacata ttcaaaagaa atagataatg caaataatga
     2521 aataaaaaaa tatttagaat taaaaagtca aaacggaaaa cttacaaaag aagaacagca
     2581 aaatctagca gctgcaagag cagaactagc actttataaa acaaaaacaa aagaattaac
     2641 tgatgttgag agagaaagac aaaaagagat aaaaagaaat attgctatca caagagatat
     2701 gcaaaatctt caaagagctt caataaatga tgcaagaact gaaatagaaa ctaaaagaaa
     2761 agtaatagag ttagaaaagc aaagcatagc ttctaaaaat aatttagaaa aatcaacttc
     2821 aaatttagca aatactacaa tcagatattt aagatgggca ggtacaatag ctggagcagt
     2881 ttatgcaggt aaaagagctt gggatgcaac tattggaagt ggtatagcag taaataaaat
     2941 aatcgaaaat aacacttatg gtatagctgc attaatatca gcaaatactc aaatggtaga
     3001 ttctttaggt aatacactta cacctcttca aaaatttgtt atggggcaag aagatgctaa
     3061 aaaggtatta gcagagctta gaaaagagtc agtaaaaaca gctgcaacat tcccacaatt
     3121 aattgaaatt ttccaacaag gtataggtaa aactttatca atgggagatt cttttggtgc
     3181 aacaacagaa gagatagaaa aaaacacaat taagttatca gcaagaatgt caaactttgc
     3241 aaatgctatt ggtatgccaa tggacagagt aaaagaagaa atgagatcat tagtatctgg
     3301 taatgcttca acagattctc taatctctac tatcattttt ggaagtccag gtgaagcaaa
     3361 tagtgcaata agagaagcag aaaaaaaagt aaatggtgta gctgaattat tagataaaaa
     3421 attcaaacca tttgatattc tagcagatac aaaaactttt gataaatcta ttttatctat
     3481 acaagatgct tggtcaagag caatgggaga tatggtagat aaatctggaa tgtttagaga
     3541 tattacaaat ttattctatg gaatgtcaga acaaataata agcaacacag atggaataat
     3601 aaaatctttt gattcactat ataattctgt aaaaaatata gtaagtattt ttggcgatat
     3661 tgcaatagct ggagcaatct tttttatagg aagtaagctt gataaaggtt tggtttcagc
     3721 agcaagactt gtagcaggat ttggagcagc agctggtggt gcgactggct tgatgttgca
     3781 attaactttg ggtgtaaatg ctttaggatt agcactcaaa ggtttagcta tggcaaatcc
     3841 ttttacagct cttgcagttg caggtacagg cttatttaca gtatttagtg caataaatga
     3901 tgaaatcaga aaaatgcaac taaatggtaa aacagtagaa gagtacgaaa a
//
LOCUS       DAOQAN010000036         3938 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_30279_length_3938_cov_10.327582, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000036 DAOQAN010000000
VERSION     DAOQAN010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 3938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3938
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30279_length_3938_cov_10.327582"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(90..596)
                     /locus_tag="PKO10_01050"
     CDS             complement(90..596)
                     /locus_tag="PKO10_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW65781.1"
                     /translation="MNNQIFEEVKSIAKHYNIVFSDENDMEQCKQEFADKVDWEWISE
                     YQKLSEGFIREFAEVDWYYISEYQKLSEGFIREFADKVDWYYISIYQKLSEGFIREFA
                     DKVDWYYISMYQKLSEGFIREFSDRVDWHCISRRQKLSEEFIREFSNKVNWDYISKYQ
                     KLSEEFIR"
     gene            complement(612..1661)
                     /locus_tag="PKO10_01055"
     CDS             complement(612..1661)
                     /locus_tag="PKO10_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW65782.1"
                     /translation="MNNQVFEEMKSIAKDYYIVFLDENDIEKCKYELVNVNNVNWYNI
                     SQNQKLSEEFIREFADEVDWCYISIYQKLSEEFIREFASKVDWHHISINQKLSEGFIR
                     EFADKVNWWYISACQKLSEEFIREFSSKVYWINISINQKLSKGFIREFASNVNWICIS
                     REQKLSEGFIREFADKVDWTCISVYQKLSEEFIRESSNKVNWDCISKYQKLSKSFIKE
                     NNLTISKDNWLYKTKKEKLSYLKKHCKDIYEIVDDKYIIAYKSTKFDGYSTYNFQYQY
                     KENEVYETHCDCNIDNNASFGLSVWTKEKALEHYKLGKLFKVHINIEDIGAIVHNNKK
                     IRCSKLTVVSEESFE"
     gene            complement(1673..2512)
                     /locus_tag="PKO10_01060"
     CDS             complement(1673..2512)
                     /locus_tag="PKO10_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW65783.1"
                     /translation="MNNQVFEEVKLIAKKYDIVFSDENDIEKCRQKFVDKVSWNNISV
                     YQKLSEEFIREFANRLRWINISTYQNLSEEFIKEFADRVYWHHNISAYQKLSEEFIRE
                     FADKVCWDCISFYQKLSEEFIREFASKIDWKYISRYQKLSKEFIQEHKLIISKDNWLY
                     KTKEDKLSYLKENCKDIYEIVDDNYIIAYKSTKIDGYSTYNFQYQYKVDETYESHCDC
                     NIDNENSFGLSAWTKEKALEYYNEGKLFKVHINIEDIGAIIHDNNKIRCSKLTVISEE
                     TFK"
     gene            complement(2524..3414)
                     /locus_tag="PKO10_01065"
     CDS             complement(2524..3414)
                     /locus_tag="PKO10_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW65784.1"
                     /translation="MNNQVFEEMKSIAKDYNIVFSDENDIKICKKIKWDCISKYQKLS
                     EEFIREFADKVDWYNVSAYQKLSESFIREFANKVYWRDISYKQKLSEEFIREFSDKVD
                     WYWISFYQKLSEEFIREFADRIDWHHISMYQKLSEEFIREFADKVDWNNISRYQKLSE
                     SFIKEYKLTISKYNWLYKTKEDKLSYLKENCNSIYEIVDDDYIVAYKSTLLNGYSTYN
                     FQYQYKVNGIYESHCDCNINNENSFGLSAWTKEKALKHYNKGKLFKVHINIEDIGAIV
                     CNNKKIRCSKLTIVSEESFK"
     gene            complement(3431..>3938)
                     /locus_tag="PKO10_01070"
     CDS             complement(3431..>3938)
                     /locus_tag="PKO10_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW65785.1"
                     /translation="SKYQKLSEGFIREFSDKVNLYWNCISSNQKLSESFIKEYNLTIP
                     KYNWLYKTKEEKLSYLKKHCSNIYKIVDNNYIVAYKSTKIEGYSIYNFQYQYKVGEIY
                     ESHCDCNIDNENSFGLSAWTKEKALEYYDEGKLFKVHINIEDIGAIVHDNMKIRCSKL
                     TVISEESF"
BASE COUNT         1319 a          607 c          349 g         1663 t
ORIGIN      
        1 cttgaaattg ttaaattgta ttctttaata aattcttttg atagtttttg atattctgaa
       61 atatattccc aatatatttt atcagaaaat tatcgaataa attcttctga taatttttga
      121 tactttgaaa tataatccca atttacttta ttggaaaatt ctcgaataaa ttcctctgat
      181 aatttttgac gtcttgaaat acaatgccaa tctactctat cagaaaattc tcgaataaat
      241 ccttctgata atttttgata cattgaaata taataccaat ctactttatc agcaaattct
      301 cgaataaacc cttctgataa tttttgatat attgaaatat aataccaatc tactttatca
      361 gcaaattctc gaataaaccc ttctgatagt ttttgatatt ctgaaatata ataccaatct
      421 acttcagcaa attctcgaat aaacccttct gatagttttt gatattctga aatccattcc
      481 caatctactt tatcagcaaa ttcttgtttg cattgttcca tatcattttc atctgagaaa
      541 acaatattat aatgttttgc aattgatttt acttcttcaa aaatttgatt gttcattagc
      601 attctttctt tctattcaaa agattcttca gacacaacag tcaacttact gcatcgaatt
      661 ttcttattgt tatgaacaat cgccccaata tcctctatat taatatgaac tttgaatagt
      721 tttcctaatt tataatgttc caatgctttt tcttttgtcc atactgataa cccaaatgaa
      781 gcattattat ctatattaca atcacaatga gtttcatata cctcgttttc tttatattga
      841 tattgaaaat tatatgtaga atatccatca aattttgttg atttatatgc aataatatat
      901 ttatcatcta caatttcata aatatcttta caatgctttt tcaaataaga tagcttttct
      961 ttctttgttt tgtataacca attatctttt gatattgtta aattgttttc tttaataaaa
     1021 gattttgata gtttttgata tttcgaaata caatcccaat ttactttatt agaagattct
     1081 cgaataaatt cttctgataa tttttgataa actgaaatac aagtccaatc tactttatca
     1141 gcaaattctc taataaatcc ttctgataac ttttgctctc gtgaaataca aatccaattt
     1201 acattactag caaattctcg aataaatcct tttgatagtt tttgattaat tgaaatatta
     1261 atccaatata ctttactaga aaattctcga ataaattcct ccgatagttt ttgacaagct
     1321 gaaatatacc accaatttac tttatcagca aattctctaa taaatccttc tgatagtttt
     1381 tgattaattg aaatatgatg ccaatctact ttactagcaa attctctaat gaattcttct
     1441 gatagctttt gatatattga aatataacac caatctactt catcagcaaa ttctctaata
     1501 aattcttcag ataacttttg attttgtgaa atattatacc aatttacatt attaacatta
     1561 acaagttcat atttacattt ttcaatatca ttttcatcca agaaaacaat atagtaatct
     1621 tttgcaattg atttcatttc ttcaaaaact tgattattca ttatatttct ttctatttaa
     1681 aagtctcttc agaaataaca gttaatttgc tgcatcgaat tttgttatta tcatgaataa
     1741 tggccccaat atcttctata ttaatatgaa ctttaaacaa ttttccttca ttataatatt
     1801 ccaatgcttt ttcttttgtc cacgctgata atccaaatga attctcatta tctatattac
     1861 aatcacaatg agattcatat gtttcatcta ccttatattg atattgaaaa ttgtatgtag
     1921 aatatccatc aatttttgtt gacttgtacg ctattatata attatcatct actatctcat
     1981 aaatatcttt acaattttct ttcaaataag atagcttatc ttcttttgtt ttgtataacc
     2041 aattatcttt tgatattatt aatttatgtt cttgaataaa ttcttttgat agtttttgat
     2101 atcttgaaat atatttccaa tctattttac tagcaaattc tcgaataaat tcttctgata
     2161 atttttgata gaatgaaata caatcccaac atactttatc agcaaactct cgaataaatt
     2221 cctcagatag tttttgatag gctgaaatat tatgatgcca atatacccta tcagcaaatt
     2281 ctttgataaa ttcttctgat agattttgat aagttgaaat attaatccat cttaatctat
     2341 tagcaaattc tcgaataaat tcttctgata atttttgata tactgaaata ttattccaac
     2401 ttactttatc aacaaatttt tgtctacatt tttcaatatc attttcatct gagaaaacaa
     2461 tatcatactt ttttgcaatt aatttaactt cttcgaaaac ttgattgttc attatatcta
     2521 ttcctattta aaagattctt cagaaacaat tgtcaactta ctacaacgaa ttttcttatt
     2581 attacaaaca attgctccaa tatcctctat attaatatga actttgaata gtttcccttt
     2641 attataatgt tttaatgctt tttctttagt ccaggctgac aatccgaatg aattttcatt
     2701 atttatattg caatcacaat gagattcata tattccattt accttatatt gatattgaaa
     2761 attatatgtg gaatatccgt tgagtagtgt tgatttatat gctactatat aatcatcatc
     2821 tactatttca taaatgctat tacaattctc ttttaaataa gatagcttat cttcttttgt
     2881 tttatacaac caattatatt ttgaaattgt taatttgtat tctttaataa aagattctga
     2941 tagtttttga tatcttgaga tattattcca atctacttta tcagcaaatt ctctaataaa
     3001 ttcttctgat agtttttgat acattgaaat atggtgccaa tctattctat cagcaaattc
     3061 tctaataaat tcttctgata gtttttgata aaatgaaatc caataccaat ctactttatc
     3121 agaaaattct cgaataaatt cttctgatag tttttgtttg tatgaaatat cacgccaata
     3181 tactttatta gcaaattctc gaataaaaga ttctgatagt ttttgataag ctgaaacatt
     3241 ataccaatct actttatcag caaattctct aataaattct tctgatagtt tttgatattt
     3301 tgaaatacag tcccatttta ttttcttaca tattttaata tcattttcat ctgaaaaaac
     3361 aatattataa tcttttgcaa ttgatttcat ttcttcgaaa acttgattgt tcatctatat
     3421 ctcttcttaa tcaaaaagat tcttcagaaa taacagttaa tttactacaa cgaattttca
     3481 tattgtcatg aacaatagct ccaatgtctt ctatattaat atgtaccttg aatagttttc
     3541 cttcatcata atattctaat gctttttctt tagtccaggc agataatcca aaagaattct
     3601 cattatctat attacagtca caatgagatt catatatctc acctacttta tattgatact
     3661 gaaaattata tatggaatat ccttcaattt ttgttgattt atatgctact atataattat
     3721 tatctacaat tttataaatg ttgctacaat gttttttcaa ataagataac ttttcttctt
     3781 ttgttttgta taaccaattg tattttggta ttgttaaatt gtattcttta ataaaagatt
     3841 ctgatagttt ttgattactt gaaatacaat tccaatacaa atttacttta tcagaaaatt
     3901 ctctaataaa tccttctgat aatttttgat attttgaa
//
LOCUS       DAOQAN010000037         3812 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_31771_length_3812_cov_3.300772, whole genome shotgun sequence.
ACCESSION   DAOQAN010000037 DAOQAN010000000
VERSION     DAOQAN010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 3812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3812
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31771_length_3812_cov_3.300772"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1761)
                     /gene="dxs"
                     /locus_tag="PKO10_01075"
     CDS             complement(<1..1761)
                     /gene="dxs"
                     /locus_tag="PKO10_01075"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HNW65786.1"
                     /translation="MQIKGKSLKELDELSSKIRERIIDVVSRKGGHFSSTLGAVELTI
                     AMHYVFNVENDPFIFDVSHQCYPHKLLTQRWEEFETIRQFGGLSGFTKPNENKADYFV
                     AGHSSTSISLAVGAAKAIKLKGENRIPVVMIGDGSMSAGMVYEALNELGDLKLPVVII
                     LNDNEMSIAKPIGAISKYLSKLLAGKFYQSFKTSVDKFIRNNMPEGTTYLAKRVENSF
                     KLITPGILFEEMGIDYIGPIDGHDIEEIIEVLEIAKSMNKPVIVHARTVKGKGYKIAE
                     GQHEHWHGVGPFNVEDGEFIKKGVEKSATAVFSDALFNLASKYENIVGVTAAMPSGTG
                     MDKLIKEFPSRFWDVAIAEQHAITSMAAMAKEGFKPFVTIYSTFLQRGFDQIIHDVCI
                     MNLPVIFAMDRAGIVGNDGETHQGAFDISFLRFIPNMVLFAPRDNETLEYSLEFAYTL
                     NSPCAIRYPRGAFSKLDFKASKFELGKSELLKNGTSNKLFIGYGSGVAKAIEVEKLHK
                     EDIAILDLRFVKPLDIDTLVLLSKKYDSWYIFSDSCKQGGVLSAILEALSEKNITNIS
                     LKSFEYEDSFIEHGDTKRVEE"
     gene            complement(1771..2874)
                     /gene="hisC"
                     /locus_tag="PKO10_01080"
     CDS             complement(1771..2874)
                     /gene="hisC"
                     /locus_tag="PKO10_01080"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014475306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HNW65787.1"
                     /translation="MKFNEVLKNLSTYEAGKPIELVVREYGIDPKEVVKLASNENPYG
                     TSPKVVAKIETLAKNMYLYPDDSMFELKEALANKFKLESKNVIIGSGSDQILEFCIHA
                     KCKDKSNVLMAKTTFAMYEIYAKQVGANIIKTESSQHNLKEFSDLYKKHGADVIFLCI
                     PNNPLGECLDKDEVYDFLKTIDEDTLVVVDGAYQEYASFKDEKKRICPKDLIEKFLNT
                     VYLGTFSKAYALGGMRVGYGLAKEEIISTFYKIRAPFNITTLSLAAAIEALKDEEFVK
                     DCIEKNFIEMKRYEKYCQDNGFEYIPSYTNFITILLKNFISKTVAQKLLEKGMIVRDL
                     TGYGLNAIRITIGTNKQNTKLFKLLDEVLEELK"
     gene            complement(2888..>3812)
                     /locus_tag="PKO10_01085"
     CDS             complement(2888..>3812)
                     /locus_tag="PKO10_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HNW65788.1"
                     /translation="KAIIDRLEKINQDKNGFLNRSAIEALFLEIFAISRNIELPENIG
                     YLGPQGSFTHQAAESRFGAMSSYVSISSIKGIFKELESKKIKFGVVPIENSSNGIVND
                     TINGFTNFNSKIVAEVILNIHHTLATTCDKISDIKKIYSKDIAFDQCRKFLTNFGLDE
                     VELIPVESTTKAAKLAANEPNSAAICPHVAAKLYNLPILFENIEDKDNNKTRFFILSD
                     FENSPSGNDKTSILVNLPDEQGVLVRFLNDFNNAGINLTKIKSHIVEGNSIFFIDFDG
                     HKDDENVKKVLEKHKSSIKVLGSYVKEIKDI"
BASE COUNT         1251 a          651 c          423 g         1487 t
ORIGIN      
        1 ttcttcaact ctttttgtat ccccatgttc tataaaacta tcttcatatt caaaagattt
       61 taaagatata tttgtaatgt ttttttcact taatgcttct agtatagcac ttaaaactcc
      121 accttgttta catgagtcac taaaaatata ccaagagtcg tatttttttg ataaaagtac
      181 taaagtatca atatctaatg gttttacaaa tcttaaatct aaaatagcaa tatcttcttt
      241 atgtaatttt tcaacttcaa ttgcttttgc tacaccactt ccatatccaa taaagagttt
      301 atttgaagta ccatttttaa gtaattctga ttttcctaat tcaaattttg aagctttaaa
      361 atcaagttta gaaaatgcac ctcttggata tctaatagcg caaggactat ttaaagtata
      421 agcaaactct aaagagtatt ccaatgtttc attatctctt ggagcaaata aaaccatatt
      481 tggaataaat cttaaaaaac taatatcaaa agctccttga tgtgtttcac catcatttcc
      541 aacaattcca gctctatcca tagcaaatat aacaggtaga ttcataatac aaacatcatg
      601 aataatctgg tcaaaacctc tttgtaaaaa tgtagagtat attgtaacaa atggtttaaa
      661 tccttctttt gccattgcag ccattgaagt aattgcatgt tgttcggcaa ttgctacatc
      721 ccaaaaacga gaaggaaact cttttattaa tttatccata ccagttccac ttggcatggc
      781 agcagttaca ccaacaatat tttcatattt agaagctaaa ttaaaaagtg catctgaaaa
      841 aactgctgta gcactttttt ctacaccttt ttttataaat tcaccatctt caacgttaaa
      901 aggtccaact ccatgccaat gctcatgttg tccttcagca attttatacc cctttccttt
      961 tactgttctt gcatgaacaa ttacaggttt attcatagat ttggcaattt ctaaaacttc
     1021 aataatctct tcaatatcat gcccatcaat aggaccaata taatcaattc ccatctcttc
     1081 aaataaaatt ccaggagtta ttaatttaaa agagttttcg actctttttg ctagatatgt
     1141 tgtaccttct ggcatattgt tacgtataaa tttatcaaca cttgttttaa aactttgata
     1201 aaattttcca gctaaaagtt ttgatagata ttttgaaatc gcacctattg gttttgctat
     1261 actcatttca ttatcattta gaattattac aactggaagt tttaaatctc ctaattcgtt
     1321 tagagcttca taaaccattc cagcactcat agaaccatca cctatcataa caactgggat
     1381 tctattttca ccttttagtt ttatagcttt tgctgctcca acagcaagag aaattgaagt
     1441 tgaactatgt ccagctacga aataatcagc tttattctca tttggttttg taaaaccact
     1501 taaaccacca aattgtctaa tagtttcaaa ctcttcccat ctttgtgtta gaagtttatg
     1561 aggataacat tgatgtgaaa catcaaaaat aaatgggtca ttttctacat taaaaacata
     1621 atgcattgca atagttaatt caactgcacc taaagttgaa gaaaaatgtc caccttttct
     1681 tgaaacaaca tcaataattc tctctcgtat tttagaagat aactcatcta actcttttaa
     1741 tgattttcct tttatttgca tcactaaact ctattttaac tcttctaaaa cttcatccaa
     1801 aagtttaaat agttttgtat tttgtttatt tgttccaata gttattctaa ttgcatttaa
     1861 tccatagcca gttaaatctc gaacaatcat acctttttca agaagttttt gagctacagt
     1921 ttttgatata aaatttttca ataaaattgt aataaaattt gtatatgaag gaatatattc
     1981 aaaaccatta tcttgacaat atttttcata tcttttcatt tcaataaagt ttttttctat
     2041 acaatctttt acaaactctt catcttttaa agcttcaata gctgctgcta aacttaatgt
     2101 tgtgatatta aaaggagctc taattttata aaaagttgaa ataatttctt ctttagctaa
     2161 accatatcca actctcattc ctccaagggc ataagctttt gaaaaagtac ctaaatatac
     2221 tgtatttagg aatttttcaa ttaaatcttt tggacaaatt ctttttttct catctttaaa
     2281 acttgcatac tcttgataag caccgtcaac tacaactaat gtatcttcat caatagtttt
     2341 taaaaaatca taaacttcat ctttgtctaa gcactctcct aaaggattgt ttggaatgca
     2401 aagaaaaatt acatcagcac catgtttttt ataaaggtca gaaaactctt ttaaattgtg
     2461 ttggctactt tcagttttta taatatttgc accaacttgc tttgcataaa tttcatacat
     2521 agcaaaagtt gttttagcca ttaatacatt tgatttatct ttacattttg catgaataca
     2581 aaattctaaa atttgatcac ttcctgaacc aataattaca tttttacttt caagtttaaa
     2641 tttattcgct aatgcttctt ttaattcaaa catagaatca tctggatata aatacatatt
     2701 ttttgcaaga gtctcaattt ttgcaacaac ttttggactt gttccatatg gattttcatt
     2761 cgaagctagt tttacaacct cttttggatc tattccatac tctcttacaa ctaattctat
     2821 tggtttccca gcttcataag ttgataaatt ttttaatact tcattgaatt tcatttttat
     2881 tttcctttta aatatctttt atctctttta cataagagcc taaaacttta atactagatt
     2941 tatgtttttc aagaactttt ttaacatttt catcatcttt gtgtccatca aagtctatga
     3001 aaaatataga gtttccttca acaatatgtg attttatttt tgttaaattt attcctgcat
     3061 tattaaaatc atttaaaaat cttaccaaaa caccttgttc atcaggaaga tttactaaaa
     3121 ttgaagtttt atcatttcca cttggactat tttcaaaatc acttaagata aaaaatcttg
     3181 ttttattgtt gtctttatct tctatatttt caaataagat tggaagatta taaagttttg
     3241 cagcaacatg tggacaaatt gctgcactat ttggttcatt tgctgcaagt tttgcagctt
     3301 ttgttgttga ttctacagga ataagttcaa cttcgtcaag tccaaaattt gttaaaaact
     3361 ttctacattg atcaaatgct atatcttttg aatatatttt ctttatatca gaaattttat
     3421 cacaagttgt tgcaagtgtg tgatgaatat ttaatataac ttcagcaact atttttgagt
     3481 taaaatttgt aaaaccattt attgtatcgt ttactatacc atttgaagag ttttctattg
     3541 gtacaactcc aaattttatt tttttagact ctaactcttt gaaaattcct tttatagaac
     3601 taatagaaac ataagagctc attgctccaa atctgctctc agcagcttga tgtgtgaagc
     3661 ttccttgagg acctaaatag ccaatatttt caggtaactc tatatttctt gatattgcaa
     3721 aaatctctaa aaaaagagct tcaattgcac ttctatttaa gaaaccattt ttgtcttgat
     3781 ttattttttc aagtcggtca ataattgctt tt
//
LOCUS       DAOQAN010000038         3795 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_31975_length_3795_cov_4.304011, whole genome shotgun sequence.
ACCESSION   DAOQAN010000038 DAOQAN010000000
VERSION     DAOQAN010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3795
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31975_length_3795_cov_4.304011"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="PKO10_01090"
     CDS             <1..208
                     /locus_tag="PKO10_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW65789.1"
                     /translation="CKKCGQYGGKLHAHHKKPFSIILKQNNITTIKEALNCKELWKKS
                     NGVTLCIKCHKKTDSYLWKNNVNS"
     gene            278..997
                     /locus_tag="PKO10_01095"
     CDS             278..997
                     /locus_tag="PKO10_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW65790.1"
                     /translation="MRYSKDSGYGQMFGKINAGKVFVLAATTDKNIQRLQELFGYDAD
                     GVARLYSTWATAIAATVAGRGDKILVSSNFTTAPTLAELATMNTNGVLAEPLGGKQGN
                     AYVATRAAATLPATAATPYFTVTGKVRILDIIGEVTTVVQTQTCNAKLIANPTVGADV
                     DMCAVLDITAAAVGSLFSITGTLANAMVKTVSACGVAQAAPLIVTAGTIDFSTSATNT
                     GATKWIVRYEPIDQGAMVLPA"
     gene            1072..1467
                     /locus_tag="PKO10_01100"
     CDS             1072..1467
                     /locus_tag="PKO10_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW65791.1"
                     /translation="MRNFQQELTIFTAKGATGTGLAHKVEDFQHVLLTLSSASNANFT
                     IKFQASNSDTCPDFSSAQSVTNRWDYIQVKDLQSGSAIGGDTGVAFAGTDDVRLFEVN
                     SSGQTWICATITARSAGTVTLTAKPFSNG"
     gene            1473..2063
                     /locus_tag="PKO10_01105"
     CDS             1473..2063
                     /locus_tag="PKO10_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW65792.1"
                     /translation="MRNTSNEQYILKARTQYLTYNTLTDAFTVGDTITGTSSGKTAVV
                     LADDGVSVLTVGSVSGAFTADEAITGALSGAAKYESISSLTGNILQVEDWKFILFTLS
                     SSGTGDFTIKFQASMSDDCPDFSAAKSATNRWDYIQVKDMQSGSAVDGDTGVTWTADD
                     VVRYETIFSGYKWVCATLTTYNAGLITLTAKLCDNK"
     gene            2065..2928
                     /locus_tag="PKO10_01110"
     CDS             2065..2928
                     /locus_tag="PKO10_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW65793.1"
                     /translation="MTKISQDELEKIEKQIIEAQNKLSDIMTAINRERLVEEKITKSN
                     QKSKDEIKKIEEELEKITNKFNFETGEIQKKEDELDVIISGLEKEINEKNTSLNNIKK
                     MVQTEEDNLISIKDKIFKEISSGKKIQEDNEKKGKEILDGLKHEIYMNQEKLDKLKHE
                     ISTNEIVLDGINKKIESLNIELGIREVELESVHSIITNFGVEKEKYIEKIDELKDTII
                     ELGEGIKGYEFELSEIKKQTDIAKSELFDIEKEKSDFIKAKFDFARQKEDLQMKEEFI
                     KEKYQQAGISY"
     gene            2935..>3795
                     /locus_tag="PKO10_01115"
     CDS             2935..>3795
                     /locus_tag="PKO10_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW65794.1"
                     /translation="MRRDELWNLRAGGYIEPLYPGYNILVRGSNKYVNFGVTVGDSGY
                     GLRDNSGTMQFKNNSGSWTDIGSGSTGSDEKVKLNAEDPTAGYLDDKIIVYGFVTGTP
                     WTLEGYITDISGQDLSLADNSTSQFITIDDVPTYQGDGITITGTGTALDPFVAHSAPT
                     PTTWDAIGGDQTDVSLSGFTNDLDTGDFTNNAGFIIGADVPSNETDPVYESEKANYEL
                     VANKSTNVVTDAASDTKYPSVKAIKEYADSKVEDSITDGVTTKAPSQNAVFDALAGKA
                     PTDSPTFTGNT"
BASE COUNT         1451 a          482 c          726 g         1136 t
ORIGIN      
        1 ttgtaaaaaa tgtggtcagt atggtggaaa acttcatgct catcataaaa aaccatttag
       61 tattatttta aaacaaaata atataactac aattaaagaa gcattgaatt gtaaagaatt
      121 gtggaaaaaa tctaatggtg taactctttg tataaaatgt cataaaaaaa cagattctta
      181 tttatggaaa aataatgtta atagttaatt aatttagggt tatgagagag taaatctctt
      241 taggtcggtg gtggacttct aaattgaata aaaaaatatg agatattcaa aagattctgg
      301 atatggtcaa atgtttggta agataaatgc tggtaaagta tttgttctag ctgcaacaac
      361 tgacaaaaat attcaaagat tacaagaatt gtttggctat gatgcagatg gtgtagctcg
      421 tctatactca acttgggcaa cagctattgc tgctacagtt gctggtcgtg gtgataaaat
      481 tcttgtttca tctaacttca caacagctcc aacattagct gaattggcaa caatgaatac
      541 taatggagtt cttgcagaac ctcttggtgg taagcaagga aatgcttatg tagcaactcg
      601 tgcagcagca acacttccag caacagcagc aactccttat tttactgtta caggtaaagt
      661 aagaatatta gatattattg gagaagttac aacagttgtt caaacacaaa cttgtaatgc
      721 aaaattaatt gcaaatccaa cagttggagc agatgttgat atgtgtgctg tattagatat
      781 tacagcagca gcagtagggt cactattttc aattactgga acattagcaa atgctatggt
      841 taaaacagta agtgcatgtg gtgtagctca agcagcacct ttaattgtta cagcaggcac
      901 tattgatttt agtacatcag caacaaatac cggtgctaca aaatggatag ttcgttatga
      961 acctatcgat caaggtgcta tggtattacc agcttaaatt tgtgttttcc cccagcagct
     1021 tatgctgttg ggttgagaac agaaatatta ctattaattt aaaaaaatat tatgagaaac
     1081 tttcaacagg aattaactat atttacagcg aaaggagcaa ctggaactgg attagcacat
     1141 aaagttgagg attttcaaca tgtgctttta actctttcat cagcttcaaa tgctaatttt
     1201 actattaaat ttcaagcatc taattctgac acttgtccag attttagttc agctcaaagt
     1261 gtaactaata gatgggatta tatacaagta aaagaccttc agagtggatc agcaattggt
     1321 ggagatactg gagtagcatt tgcagggaca gatgatgttc gtttatttga ggtaaactca
     1381 agtgggcaaa catggatttg tgcaactatt acagcaaggt cagctggaac agttacttta
     1441 acagcaaaac catttagtaa tggataaatt ttatgagaaa tacatcaaac gaacagtata
     1501 tattgaaagc cagaactcaa tatttaactt ataatacttt aactgatgct tttactgttg
     1561 gagatacaat tacaggaact agttcaggga aaacagcagt tgttttggca gatgatggag
     1621 ttagtgtttt aacagtcggt tcagtttctg gtgcatttac tgcagacgaa gcaataactg
     1681 gagcattaag tggagcagca aaatatgaat caataagtag tttaacagga aatattttac
     1741 aagtagaaga ctggaaattt atattattta ctttatcctc ttctgggaca ggtgatttta
     1801 ctattaaatt tcaagcttca atgtcagatg attgtcctga tttttcggca gctaaatcag
     1861 caacaaatag atgggattat attcaagtaa aagatatgca gtccggatca gctgttgatg
     1921 gtgatactgg ggtcacttgg actgcagatg atgtagttag atacgaaaca atatttagtg
     1981 gctataaatg ggtatgtgcg acattaacta cctataatgc tgggttgatt acattaacag
     2041 ctaaattatg tgacaacaaa taatatgact aaaatatcac aagatgaact agaaaaaata
     2101 gagaaacaaa taattgaagc tcaaaataaa ttgagtgata ttatgactgc tattaataga
     2161 gaaagattag tagaagaaaa aattactaaa agtaatcaaa aatcaaaaga cgagattaaa
     2221 aaaattgaag aagaacttga aaaaattaca aataaattta attttgaaac aggagaaatc
     2281 caaaagaaag aggatgaatt agatgttatt atttctggat tagagaaaga gattaatgaa
     2341 aagaatactt ctttaaataa tataaaaaag atggttcaga ctgaagaaga taatctaata
     2401 tctattaaag ataaaatttt taaagaaata agttctggta aaaaaattca agaagataat
     2461 gagaaaaaag gtaaagaaat tcttgatgga ttaaagcatg agatttatat gaatcaagaa
     2521 aaattagata aattaaaaca tgaaatttct actaatgaaa ttgttcttga tggaataaat
     2581 aaaaaaattg aatcattaaa tattgaactt ggaattagag aagttgagct tgagagtgtc
     2641 catagtatta taactaattt tggagttgaa aaagaaaagt atatagaaaa aattgatgag
     2701 cttaaagata ccataatcga acttggagaa ggaattaaag gttatgagtt tgaattgtct
     2761 gaaattaaaa aacaaactga tatagctaaa tcagaactct ttgatattga aaaagaaaaa
     2821 tctgatttta ttaaagcaaa gtttgatttt gcaagacaaa aagaagacct tcaaatgaaa
     2881 gaagaattta tcaaagaaaa atatcaacaa gctggtatca gctactaaaa cattatgaga
     2941 agagatgaac tatggaattt aagagcagga ggatatatag agccacttta tcctggttat
     3001 aatattcttg ttcgtggttc taataaatat gtaaactttg gagttactgt tggtgattct
     3061 ggttatggac ttcgtgataa ttctggaact atgcaattta aaaacaatag tggttcttgg
     3121 acagatattg gttctggttc aactggttca gatgaaaaag tgaaattaaa tgcagaagat
     3181 ccaacggcag gatatttaga tgataaaatt atagtatatg gttttgtaac aggaacacct
     3241 tggactttag agggatatat tactgatata agtggccaag atttaagttt agctgacaat
     3301 agtacaagtc agtttattac tattgatgat gtacctactt atcaaggaga tggaataact
     3361 attacaggaa ctggtacagc attagatcct ttcgtggcac attctgcacc tacacctaca
     3421 acttgggatg caataggtgg agatcagaca gatgtttctt tgagtggttt tacaaatgat
     3481 ttagatacag gagattttac aaataatgca ggttttatta ttggtgcaga tgtaccatca
     3541 aatgaaacag atccagtata tgaaagtgaa aaagcaaatt atgaattagt tgcaaataaa
     3601 tcaacaaatg tagtaacgga tgcagccagt gatacaaaat acccaagtgt taaagcaata
     3661 aaagaatacg ctgattcaaa agtagaagac agtataacag atggagtaac aacaaaagct
     3721 ccaagtcaaa atgcagtatt tgatgcgttg gctggaaaag ctccaacaga ttctcctaca
     3781 tttacaggaa atact
//
LOCUS       DAOQAN010000039         3772 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_32273_length_3772_cov_2.529997, whole genome shotgun sequence.
ACCESSION   DAOQAN010000039 DAOQAN010000000
VERSION     DAOQAN010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 3772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3772
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32273_length_3772_cov_2.529997"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1453
                     /locus_tag="PKO10_01120"
     CDS             <1..1453
                     /locus_tag="PKO10_01120"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014472980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HNW65795.1"
                     /translation="KKALEVVDSELKKAGISRRDAFKLAGLSGAAFLAGGTEVKASTV
                     AKASEAKGKILIIGGGLAGISTAARLSNTLSEPDITIIEPNPKSVSYQAGTTLLASGI
                     YSSKDELVYETKDFLPKGVTLIKDKAVDFNPEANRVTLASGDILDYDFMIVAAGVVLD
                     FGAIKGLEEIGEAYTNGDASKILKVFGNSGITSVYNIDSAEDMWKQTQTFIERAKSGE
                     KLKAIFTAPNTAVKCGGAPKKVMYLVNSRLNEANARGNVDLTYYDNSDKLFSVKEYAD
                     AIEKQFIARDMKWNFNHNLVGVDISKKIAIFNKHWEEKGEYDKDLEEHEIVKKNQKVE
                     VPFDFLHITPPQKAPDEIGKSEIGSDKGWIPVNKETLQHVKYSNIFALGDIAAVPMGK
                     TGGTVRKQYKVLVDNLISVMEGKEPKEYFGGYTVCPLITDIGKVMLAEFDWTAKPTPS
                     FPLDPTQERWIWWLMKVYMLKPMTMHGMLSGKA"
     gene            1480..2661
                     /locus_tag="PKO10_01125"
     CDS             1480..2661
                     /locus_tag="PKO10_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012011980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNW65796.1"
                     /translation="MKKLLAIFAMVLMFLQLNAEEVVDLKEPSSEVKELMKKHKLKEV
                     DFDYVKKMIGVGNRGAVEAILIDARPQVKYQRGTIPSSLNIADTDFEVGYKQIADVSK
                     DKEIIVFCGGYACTKSAIVADLLMKKGHKNVKVYNAGEPEWSKKDYLEVDTLVVKAYF
                     ENNSALLVDARPHVKYLQETILGSISIPDTNFDKLVGRFPIDKDEKIVVFCAGYDCEK
                     SNIVADKLYKLGYKNVVVYAGGLPEWKKQSLPTTAGAKKVDAVKKEQKPEFSKNGAKL
                     GKDEGSIDGEWLKALIIDNKVPEYIQIVNVLPAKEFKKGNIKGSINIETDKLSAKEIV
                     SKLPKNKTIIFNCSAGARSIEAWMKLKKDGIDVSEIFYFDANINCKDNNCKIEVNEPL
                     E"
     gene            complement(2738..3094)
                     /gene="rplT"
                     /locus_tag="PKO10_01130"
     CDS             complement(2738..3094)
                     /gene="rplT"
                     /locus_tag="PKO10_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HNW65797.1"
                     /translation="MPRVKTGVVRRRRHKKVLKLARGFFGGRRKHFRKAKEQLERSLV
                     YAFRDRRQKKRDIRKLWIIRINAACRLNDINYSRFINGLRLSGIELDRKILANLAMND
                     SAAFASLVVSAKAALK"
     gene            complement(3192..3389)
                     /gene="rpmI"
                     /locus_tag="PKO10_01135"
     CDS             complement(3192..3389)
                     /gene="rpmI"
                     /locus_tag="PKO10_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HNW65798.1"
                     /translation="MPKMKSVKGAVKRFKVKKNGTIKRGSAFRSHILTKKTQKRKRNL
                     RGPQTVHSTNLNGVLSTLCLA"
     gene            complement(3543..>3772)
                     /gene="infC"
                     /locus_tag="PKO10_01140"
     CDS             complement(3543..>3772)
                     /gene="infC"
                     /locus_tag="PKO10_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HNW65799.1"
                     /translation="DNDINYKVKHAIEFLEEGNHVKFRVFLKGREMANPEAGIEVLRR
                     VWAMIENIAIMDKEPKLEGRYVNLLVTPKKD"
BASE COUNT         1382 a          455 c          670 g         1265 t
ORIGIN      
        1 gaaaaaagca ttagaagtag tagatagcga attaaaaaaa gctggaatct caagaagaga
       61 tgcttttaag cttgcaggtc ttagtggagc tgctttttta gctggaggaa cagaggttaa
      121 agcttcaact gttgcaaaag ctagtgaagc aaaaggaaaa atactaatta ttggtggagg
      181 tctagctgga atttcaacag cagctagatt gtcaaatact ttaagtgaac ctgatattac
      241 aataattgag ccaaatccaa aatctgtatc atatcaagct ggaactactt tgttggcaag
      301 tggaatttac tcttcaaaag atgagttagt ttatgaaaca aaagattttt taccaaaggg
      361 agtaacttta ataaaggata aagcagtaga ttttaatcct gaagcaaata gagttacttt
      421 ggcttctgga gatattttag attatgattt tatgatagtt gcagctggag ttgttttaga
      481 ctttggtgct ataaaaggtc ttgaggaaat aggagaagca tatacaaatg gcgatgcatc
      541 taaaatttta aaagtttttg gaaatagtgg aataacatct gtttataata ttgatagtgc
      601 agaggatatg tggaaacaga ctcaaacatt tatagaaaga gcaaaatcag gtgaaaaatt
      661 aaaagctatt tttacagctc caaatacagc tgtaaaatgt ggtggagcac caaaaaaagt
      721 tatgtatttg gtaaattcaa gactaaatga agcgaatgct agaggaaatg ttgatttaac
      781 atattatgat aatagcgata aactttttag tgtaaaagag tatgcagatg caattgaaaa
      841 acagtttatt gcaagagata tgaagtggaa ttttaatcat aatttagttg gagttgatat
      901 ttcaaaaaaa atagcaatat ttaataaaca ctgggaagaa aaaggtgaat atgataaaga
      961 tttagaagag catgaaattg taaagaaaaa tcaaaaagtt gaagtacctt ttgacttttt
     1021 acatataaca ccacctcaaa aagcaccaga tgagattgga aagtctgaaa taggttcaga
     1081 taaaggatgg attcctgtaa ataaagagac actacaacac gtaaaatata gcaatatttt
     1141 tgctcttgga gatatagcag ctgttcctat gggaaaaaca ggtggaactg taagaaaaca
     1201 gtataaagta ttggttgata atttgatttc agttatggaa ggaaaagaac caaaagagta
     1261 ttttggagga tatacagttt gtccacttat tacagatata ggaaaagtaa tgttagcaga
     1321 gtttgactgg acggcaaaac caactccgtc atttcctttg gacccaacac aagagagatg
     1381 gatttggtgg ttaatgaaag tgtatatgtt aaaacctatg actatgcatg gaatgctaag
     1441 tggtaaagca taattaaaat ttaaataaag gggtataaaa tgaaaaagtt attagcaatt
     1501 ttcgctatgg ttttgatgtt tcttcaatta aatgcagaag aagtggttga tttaaaagaa
     1561 ccttcaagtg aagtaaaaga gcttatgaaa aagcataaat taaaagaggt tgatttcgat
     1621 tatgttaaaa aaatgattgg agttggaaat aggggagctg tagaagcaat tttaatagat
     1681 gcaagacctc aagttaaata tcaaagagga actattcctt caagtttgaa tattgcagat
     1741 actgattttg aagttggtta taaacaaata gctgatgttt caaaagataa agagattata
     1801 gtattttgtg gtggttatgc ttgcacaaaa agtgctatag ttgcagattt attgatgaaa
     1861 aaaggtcata aaaatgttaa agtttataac gctggtgagc ctgaatggtc taaaaaagat
     1921 tacttagaag ttgatacatt ggttgttaaa gcttattttg aaaataacag tgcgttatta
     1981 gtagatgcaa gaccacatgt taaatattta caagagacaa ttttaggaag tatttcaatt
     2041 cctgatacaa attttgataa attagtagga agatttccta tagataaaga tgaaaaaatt
     2101 gtagtttttt gtgcaggtta tgattgtgaa aaatcaaata tagttgcaga taaattatat
     2161 aaattaggtt ataaaaatgt tgttgtttat gcaggaggtc ttcctgaatg gaaaaaacaa
     2221 tctcttccaa caacagctgg agctaaaaaa gttgatgcag taaaaaaaga acaaaaacca
     2281 gaatttagta aaaatggtgc aaaacttgga aaagatgaag gaagtattga tggtgagtgg
     2341 ttaaaagctc tgattattga taataaagtt ccagaatata tacaaattgt aaatgtatta
     2401 ccagcaaaag agtttaaaaa gggaaatatc aagggttcta ttaatattga aactgataag
     2461 ctttcagcaa aagagattgt ttcaaaatta cctaaaaata aaactattat ttttaactgt
     2521 agtgcaggag ctagatctat tgaagcttgg atgaaactta aaaaagatgg tattgacgtt
     2581 agcgagattt tctattttga tgcaaatata aattgtaaag ataataattg taaaattgaa
     2641 gtaaatgagc ctttagaata aaaggcttat ctattttttg atataaaaaa agggaaggtt
     2701 tttaaacctt ccctttttta ttttgtatgc aaatttatta tttaagtgct gctttagcag
     2761 atactactaa agatgcaaat gcagcagagt cattcatagc aagatttgct aagatttttc
     2821 tatcaagttc aatacctgat aatcttaaac catttatgaa tcttgagtag ttaatatcat
     2881 ttaatctaca agctgcattg attctgataa tccatagttt tctaatatct ctttttttct
     2941 gtcttctatc tctaaatgcg tatacaagac ttctttctaa ttgttcttta gcttttctaa
     3001 agtgttttct tcttccacca aagaaccctc tagctagttt taatactttc ttgtgtcttc
     3061 ttcttcttac aacaccagtt tttactcttg gcatattttt cctttcttta ccgttacttt
     3121 taataaggtg tcggatctat ccgaacttat ccatataaat atggagggac aaaattacta
     3181 tattttagta attacgctaa acataaagtt gaaagaacac catttaagtt tgtactatgt
     3241 acagtttgtg gtcctctcaa atttcttttt cttttttgcg tttttttagt taaaatgtgg
     3301 cttctaaaag cagaacctct tttgatagtt ccatttttct ttactttgaa tcttttaaca
     3361 gcacctttaa cgcttttcat ttttggcata aagaaatcct ttcatataaa tttgcatttt
     3421 tcatattgaa aaagttttgg attatactca aaaaaatatt aataaatgat taaaagatat
     3481 aaaaaatatt gactagagta tctaatattt tataaagata gtttaaaaaa gattagtttt
     3541 aactaatctt ttttaggagt tacaagaaga tttacgtatc ttccttctaa ttttggttct
     3601 ttgtccataa ttgcaatatt ttcaatcata gcccaaactc ttcttaaaac ttcaatacca
     3661 gcttctggat ttgccatctc tctacctttt agaaaaaccc taaattttac atggtttcct
     3721 tcttctaaaa attcaattgc atgttttact ttgtagttaa tgtcattgtc gg
//
LOCUS       DAOQAN010000040         3727 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_32866_length_3727_cov_3.294935, whole genome shotgun sequence.
ACCESSION   DAOQAN010000040 DAOQAN010000000
VERSION     DAOQAN010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 3727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3727
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32866_length_3727_cov_3.294935"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1018
                     /locus_tag="PKO10_01145"
     CDS             <1..1018
                     /locus_tag="PKO10_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HNW65800.1"
                     /translation="KLANYLKVRNKKVLVVACDLQRLAAVEQLKQIAASIDVDIYFDD
                     TEKNPIKIAKSAIEKAKKEHYDVVLLDTAGRLAIDEELMNELKEIKNAVNPNEIFYVA
                     DSLTGHDATKTAITFKEKIGIDGVILSKYDGDTKGGVALSIASQVQVPLRFIGIGEKM
                     PDLEVFIPDRIVSRLLGLGDIAGLAEKTSTIFDEKKAKEVSKKIKKGEFNFNDFLEQL
                     QMMSKLGSLKSIIGMIPGLSSALPALKDMDFENSVEIKRIKALIGSMTPKERENPDLL
                     NPSRKKRISTGAGLSEVQVNKILKQFKSASKMAKQLSSKGGMKGLHNMLSQVGANGMP
                     KIPR"
     gene            1097..1324
                     /gene="rpsP"
                     /locus_tag="PKO10_01150"
     CDS             1097..1324
                     /gene="rpsP"
                     /locus_tag="PKO10_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002852274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HNW65801.1"
                     /translation="MTVIRLTRMGRNKKPFYRIVVTDSRKRRDSGWIESIGYFNPVVE
                     PQVLKIDEERYNYWLSVGAKPSEKVKKLASR"
     gene            1339..1578
                     /locus_tag="PKO10_01155"
     CDS             1339..1578
                     /locus_tag="PKO10_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HNW65802.1"
                     /translation="MIINFIENYAKLVVAVPEDVVVTKEQIDENFIEITISVNSVDIG
                     KLIGKNGNMINALKTIANGCKAKDGISYKIQVVTK"
     gene            1579..2106
                     /gene="rimM"
                     /locus_tag="PKO10_01160"
     CDS             1579..2106
                     /gene="rimM"
                     /locus_tag="PKO10_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002951210.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HNW65803.1"
                     /translation="MNKVYVAKLGKTVGLQGHLRLFIDSDFPEQFKKGATFTTNRNLI
                     LKVIECNLNKDLIKFENYEDVDLAKKLTNSELFTSQEATKEYCKLKENEFFWFDLISC
                     EIFENGLLLGKVKDIHRYPLNDYLEVETSSELISKQLPKVFLIPHIFDKFIEKVDIEN
                     KKIFVKNAFDILENS"
     gene            complement(2103..3020)
                     /locus_tag="PKO10_01165"
     CDS             complement(2103..3020)
                     /locus_tag="PKO10_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW65804.1"
                     /translation="METTNTNPENQISENQEQKEDDKKDKTLLIKRVLFITIGVLLLF
                     LIILATIFIFKSEESKEPAESSPPIESSAKTNENNTENVPSEPEIKETKFDFNNLEPE
                     KLNEQLELLTNKNMEYQKEKEQKILEEEKKLSPVFNLVNNLKDKEEIKKEENSIVSSE
                     DNKKEEISKIEEIKEDKKEEIISEKKDIATSTNNETKTQNETSNINSNSKKEETTKIT
                     ESLTFVKLINVAKIKGDLNKKYFDKVIEVNANVLLCRDDENIIELYYGPFLEDEIRDN
                     LLSKLIKNGFKEAYSLEMTKEEFDKRCNY"
     gene            complement(3007..3393)
                     /locus_tag="PKO10_01170"
     CDS             complement(3007..3393)
                     /locus_tag="PKO10_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013136302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW65805.1"
                     /translation="MNLEELKKLREKNFSKHKEKKRAYYLKSKIKKEIDYEDELKDDG
                     FLKKMQEIVKSQRLYMDSRQETIKKKIEEYQILKRDYYEQNKEKRLEYDREYRERKKE
                     QLKAYRQKYYEKQKEKKLEEEKNGNN"
     gene            complement(3413..>3727)
                     /locus_tag="PKO10_01175"
     CDS             complement(3413..>3727)
                     /locus_tag="PKO10_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014469290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HNW65806.1"
                     /translation="ARDPQTGDTIELGQITLADFINPAGLAPLGDSLFMQTDASGDVV
                     QGDPTTEQFGGLRQGMVESSNVKLVNEMVDLITAQRAYEANSKAITTADSMLDTVNRL
                     KN"
BASE COUNT         1281 a          472 c          544 g         1430 t
ORIGIN      
        1 aaagcttgca aattatctta aagtaagaaa taaaaaagtt ttagttgttg cttgtgattt
       61 acaaaggctt gcagctgttg agcaattaaa acaaatcgca gcttctattg atgttgatat
      121 atattttgat gatactgaaa aaaatcctat aaaaattgca aaaagtgcta ttgaaaaagc
      181 taaaaaagag cattatgatg ttgttttatt agatacagct ggaagacttg ctattgatga
      241 agagcttatg aatgagctaa aagagattaa gaatgcagta aatccaaatg agatttttta
      301 tgttgcagat tcattaacgg gtcatgatgc aactaaaaca gctattacat ttaaagagaa
      361 aattggaata gatggagtta ttttatcaaa atatgatggt gatacaaaag gtggtgttgc
      421 tttaagtatt gctagtcaag ttcaagttcc tttaagattt attggtattg gtgaaaagat
      481 gccagatttg gaagttttta ttccagatag aatagtttca agacttttag gacttggtga
      541 tattgctggg cttgctgaaa aaacatcaac aatttttgat gagaaaaaag caaaagaagt
      601 tagtaaaaaa atcaaaaagg gtgaatttaa ctttaatgac tttttagagc agttacaaat
      661 gatgagtaag ttaggtagtt taaaatcaat tataggaatg attccaggtt taagtagtgc
      721 attgcctgct ttaaaagata tggattttga aaactctgta gagataaaaa gaattaaggc
      781 tttaattggt tctatgactc caaaagagag agaaaatcca gatttattaa atcctagcag
      841 aaagaaaaga atttcaactg gtgctggttt aagtgaagtt caagtaaata aaatattaaa
      901 acagtttaag agtgcttcaa agatggcaaa acagctttct agcaaaggtg gaatgaaagg
      961 tttacataat atgctctctc aagttggagc aaatggaatg cctaaaattc caagataatt
     1021 ttaaataaaa tagtgttgag ctataaactt tgttggagtt taaagctcaa tactataaat
     1081 aaaaaaagga taaaacatga cagtgattag attaacaaga atgggaagaa ataaaaaacc
     1141 attttataga atagttgtaa cagactcaag aaaaagaaga gattcaggtt ggattgaatc
     1201 aattggttat tttaacccag ttgtagagcc tcaagttctt aaaattgatg aagagagata
     1261 taactattgg ttaagtgttg gtgcaaaacc atcagaaaaa gttaaaaaat tagcttcaag
     1321 ataatagttt aagctaaaat gataataaat tttatagaaa actatgcaaa actagttgtt
     1381 gcagttcctg aggatgttgt tgtaacaaaa gagcaaattg atgaaaattt cattgaaatt
     1441 acaataagtg taaatagtgt tgatatagga aaacttattg gaaaaaatgg aaatatgata
     1501 aatgctttaa aaacaattgc aaatggttgc aaagcaaaag atggaatatc ttataaaata
     1561 caagtagtta caaaatagat gaataaagtt tatgttgcaa aattaggaaa aacagttggt
     1621 ttacaaggtc atttaagact ttttatagac tctgattttc ctgagcaatt taaaaaaggt
     1681 gctactttta caacaaatag aaacttgatt ttaaaagtta ttgagtgtaa tttaaataaa
     1741 gacttaataa aatttgaaaa ttatgaggat gtagatttag caaaaaaact tacaaatagt
     1801 gagcttttta caagccaaga agctacaaaa gagtattgta aattgaaaga aaatgaattt
     1861 ttctggtttg atttaatctc ttgtgaaatt tttgaaaatg gtttactttt aggaaaagta
     1921 aaagatattc atagataccc tttaaacgac tatttagaag ttgaaacttc tagtgagctt
     1981 atttcaaaac aacttccaaa agtattttta ataccacata tatttgataa gtttatagaa
     2041 aaagtagata tagaaaacaa aaaaatattt gtaaaaaatg catttgatat tttagaaaac
     2101 tcttagtagt tgcatctttt atcaaactct tcttttgtca tctctaaaga gtaagcctct
     2161 ttaaaaccat ttttaattaa tttagagagt aaattatctc ttatttcatc ttctaagaat
     2221 ggaccataat ataactctat tatattttca tcatctctac aaagcaaaac atttgcattt
     2281 acttcaatca ctttatcaaa atatttttta tttaaatcac cttttatttt agcaacattt
     2341 ataagtttta caaatgttaa agactctgtt attttagtag tttcctcttt tttagagtta
     2401 gaattgatat ttgaagtttc attttgagtt ttagtttcat tatttgtact tgttgctata
     2461 tctttttttt cagagataat ttcttctttt ttatcttctt ttatctcttc aatttttgat
     2521 atttcttctt ttttattatc ttctgaactc actatactgt tttcttcttt tttaatctct
     2581 tctttatctt ttaaattgtt tacaagatta aaaacaggag atagtttttt ctcttcttca
     2641 agaatttttt gttctttctc tttttgatac tccatatttt tatttgttaa taactctaac
     2701 tgttcattta gcttttctgg ttctaggttg ttgaagtcaa attttgtctc ttttatttct
     2761 ggttcagaag gaacattttc tgtattattt tcatttgttt tagcagaact ttcaatagga
     2821 ggagaagatt cagcaggctc tttactctct tcacttttaa aaataaatat agtagctaag
     2881 attattaaaa aaagtagtaa aacaccaatt gtaataaata aaactctttt tataagaaga
     2941 gttttatctt ttttatcatc ttctttttgt tcttgatttt cacttatttg attctctgga
     3001 tttgtattag ttgtttccat ttttctcttc ttctaatttt ttctcttttt gcttctcata
     3061 atatttttgt ctatatgctt ttaattgctc tttttttctc tctctatact ctctatcata
     3121 ttcaagccgt ttttctttat tttgttcata gtaatctctt tttaaaatct gatactcttc
     3181 tattttcttc ttaattgtct cttgcctact atccatatag agtctttgac tttttacaat
     3241 ctcttgcatt ttttttaaaa agccatcatc ttttaattca tcttcgtaat ctatctcttt
     3301 ttttatttta ctttttagat agtaggctct ttttttctct ttgtgttttg agaaattttt
     3361 ttctcttaat ttttttaact cttctaaatt cataattgat taaaagctat aattaatttt
     3421 ttaacctatt tacagtatca agcatactat cagctgttgt gattgctttt gagtttgctt
     3481 cataagctct ttgagcagta attaaatcaa ccatctcatt tacaagctta acattggaac
     3541 tttcaaccat tccttgtctt aatccaccaa actgctctgt agttgggtct ccttgtacaa
     3601 catcaccact agcatctgtt tgcataaata aagagtcacc taaaggtgct aatccagctg
     3661 gatttataaa atctgctaaa gtaatttgac ctaactctat tgtatcacca gtttgtggat
     3721 ctcttgc
//
LOCUS       DAOQAN010000041         3693 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_33293_length_3693_cov_2.973337, whole genome shotgun sequence.
ACCESSION   DAOQAN010000041 DAOQAN010000000
VERSION     DAOQAN010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 3693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3693
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33293_length_3693_cov_2.973337"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            144..686
                     /locus_tag="PKO10_01180"
     CDS             144..686
                     /locus_tag="PKO10_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014469517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HNW65807.1"
                     /translation="MAKILVPIANGFEEIEAISVIDICRRAGIEVVTAGVEDKVLIGA
                     HNIKIEMDKKIDEVKSSDFDMIVLPGGLPNAFTLAEDKDVQRLLKEFEEKNKKIGAIC
                     AAPYALHKAGVLNQNYTCYPSFEKKIKDDGYQDDKNVVTDNNVITSRGPATAIDFALE
                     IVKTLKGNDIYHQIKTGLLA"
     gene            789..>3693
                     /gene="dnaE"
                     /locus_tag="PKO10_01185"
     CDS             789..>3693
                     /gene="dnaE"
                     /locus_tag="PKO10_01185"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNW65808.1"
                     /translation="MSQIPQFTHLHLHTEYSLLDGANKIKPLAKKLKEFGMKSVAMTD
                     HGNMFGAIDFYNAMKNEGIKPIIGMEAYIHNSEDLGDKTNRQRFHLCLYAKDEIGYKN
                     LMYLSSQAYINGFYYYPRINKELLRNHSEGLVCSAACLQGEVNWHLNTQNERNVKNGA
                     KGYEEAKKVALEYKEIFGDDFYLEIMRHGIGDQHFVDDQILRISAETGIKVVATNDTH
                     YLEQKDADAHEAFMCIAMNKLYDDPNRLRHSVHEFYLKSQEQIYKLYADIPEAIEATQ
                     EIADKCNLTIKLGNPTPPNFKFTRDKLKELNIDIPEPQNEYSLENDKVLFAFECRKGL
                     EDRLKLVPQNKHQEYKDRLETEIEIINNMKFPGYMLIVWDFVKVAKDMQIPVGPGRGS
                     AAGSLVAYSLKITDIDPIPYGLLFERFLNPERVSMPDIDMDFCQSRRGEIIDYVVNQY
                     GRANVAQIITFGKLLAKGVIRDVARVLDMPYAKADAMAKLIPDELGIDLKNSWEKEPK
                     IKELCETDPLAARVWEYALALEGLNRNAGTHAAGVVISNEPLWNKTPLFKPSGLDTLA
                     TQYNGKYIEDVDLIKFDFLGLKTLTVIDEALKLIEQRHGKKIDFATVDVNDKGVYDLI
                     QTGDTLGLFQIESDGMQDLCKRLKPSNFEDIVAVLALYRPGPMESGMLNDFIERKHGR
                     AKIDYFHDELEIALKPILENTYGVIVYQEQVMQIVQSVGGFSLGGADLVRRAMGKKIK
                     EEMDKLKGQFASGAEQKGFTKAYAEELFDLIVKFAGYGFNKSHSAAYALITFYTSYLK
                     CYYPAEFMAALLTLEKDNTDKVVRYVDEVKRLGLELFAPDINKSDLVFSARVVDNREV
                     VMFGMGAIKGAGDVAINSILNAREDGDFKNLSDFVSRIDASKVNKRVIESLAKAGVFD
                     SFGYSRNALLSQIEKIVDGASKASNAKKMSSGSLFGDSTELTSIEIELDNLPEY"
BASE COUNT         1401 a          465 c          687 g         1140 t
ORIGIN      
        1 aataaacttc aattttgagt taagcttcat tcttgaagcc ctttaggatt tgctacttta
       61 acaatttttt ataaattgtg ttaaaaatag ttaaaaaata ttagttcctt tctaaatagg
      121 aactatttaa aaaaggataa tttatggcaa aaattttagt tccaatagca aatggatttg
      181 aagagataga ggcaatttca gttattgata tttgcagaag agcaggaata gaagttgtaa
      241 ctgctggagt tgaagataaa gttttaattg gtgctcataa tataaaaatt gagatggata
      301 agaaaattga tgaagtaaaa tcaagtgatt ttgatatgat agttcttcca ggtggtcttc
      361 caaatgcttt tactttggct gaagataaag atgttcaaag acttttaaaa gagtttgaag
      421 agaaaaacaa aaaaattgga gctatttgtg cagctcctta tgcacttcac aaagcaggag
      481 ttttaaatca aaactatact tgctatccaa gttttgaaaa aaagataaaa gatgatggct
      541 accaagatga taaaaatgtg gttactgata ataatgtaat cacatcaaga ggaccagcaa
      601 ctgctattga ttttgcactt gaaattgtaa aaactttaaa aggtaatgat atttatcacc
      661 aaattaaaac aggtcttcta gcataagttt gtaagccttt atttaaggct tacaaacttt
      721 taattacttc aaaaacaaat tttagataga atctttagtt aaaaaataaa atagaagatt
      781 tataaaatat gtcacaaata ccacaattta cacatttaca tttacataca gaatattcac
      841 tacttgatgg tgcaaacaaa ataaaacctc ttgcaaaaaa actaaaagag tttggcatga
      901 aaagtgttgc tatgactgac catggaaata tgtttggagc aatagacttt tataatgcaa
      961 tgaaaaatga agggataaaa ccaatcattg gaatggaagc atatattcac aatagcgaag
     1021 atttaggaga taaaacaaat agacaaagat ttcacttatg cttatatgca aaagatgaga
     1081 taggttataa aaatcttatg tatcttagtt ctcaagccta tataaatggg ttttactact
     1141 atcctagaat aaataaagaa cttctaagaa accattctga aggtcttgtt tgtagtgctg
     1201 cttgtttaca aggagaggta aactggcatc taaatactca aaatgaacga aatgttaaaa
     1261 atggtgcaaa aggttatgaa gaggcaaaaa aagtagcact tgaatacaaa gagatatttg
     1321 gtgatgattt ttatttagaa attatgaggc atggtattgg tgaccagcat tttgtagatg
     1381 accagatttt aagaatctct gctgaaacag gaataaaagt tgttgcgaca aatgatactc
     1441 actatttaga acaaaaagat gctgatgcac atgaagcttt tatgtgtatt gctatgaata
     1501 aactctatga tgatccaaat agattaagac atagtgttca tgagttttat ttaaaatctc
     1561 aagaacaaat ttataagctc tacgcagata ttcctgaagc tatagaagct actcaagaaa
     1621 tagctgataa atgtaatctt acaatcaagc taggaaatcc aacaccacca aactttaaat
     1681 ttacaagaga taaattaaaa gagttaaata tagatattcc tgaaccacaa aatgagtact
     1741 ctttagaaaa tgataaggtt ttatttgcat ttgagtgcag aaaaggttta gaagatagat
     1801 taaaactagt tccacaaaat aaacatcaag agtataaaga tagattagaa acagaaatcg
     1861 aaataattaa taatatgaaa ttcccaggat atatgttaat cgtttgggat tttgtgaaag
     1921 ttgcaaaaga tatgcaaatt cctgtaggtc cagggcgagg aagtgcagct ggaagtttag
     1981 ttgcatatag tcttaaaatt acggatattg accctattcc ttatggttta ctttttgaga
     2041 gatttttaaa tccagagcgg gtaagtatgc ccgatattga tatggacttt tgccaaagcc
     2101 gtcgtggtga gattatcgat tatgttgtaa atcaatatgg acgagcaaac gttgcacaaa
     2161 ttataacttt tggtaaactt ttggcaaaag gagttatacg agatgttgca agagttttag
     2221 atatgcctta tgcaaaagct gatgctatgg caaaactaat tcctgatgag ttaggaattg
     2281 atttaaaaaa ctcttgggaa aaagagccaa aaataaaaga actttgcgaa acagatcccc
     2341 ttgcagctag agtttgggaa tatgctcttg ctttagaggg attaaataga aatgctggaa
     2401 ctcatgcagc tggagtggtt atctcaaatg aaccactttg gaataaaact ccacttttta
     2461 aaccaagcgg gcttgatact cttgcaactc aatacaatgg aaaatatatt gaagatgtag
     2521 acttaattaa attcgacttt cttggactta aaacccttac agttattgat gaagctttaa
     2581 aactaataga acaaaggcat ggtaaaaaaa tagattttgc aactgttgat gtaaatgata
     2641 aaggtgttta tgatttaatc caaacaggag atactttagg actatttcaa atagaatctg
     2701 atggtatgca agatttatgt aaaagattaa aaccatcaaa ttttgaggat attgtagccg
     2761 ttcttgcact ttatagacca ggacctatgg aatctggaat gctaaatgac tttatagaga
     2821 gaaaacacgg gcgtgcaaaa attgattact tccatgatga actagaaatt gcactaaaac
     2881 ctattttaga aaatacttat ggagttattg tttatcaaga gcaagttatg caaattgttc
     2941 aaagtgttgg aggtttttcg cttggaggag ccgatttagt aagacgagca atgggtaaaa
     3001 agattaaaga agagatggat aagctaaaag gtcaatttgc ttctggtgca gagcaaaaag
     3061 gatttacaaa agcctatgca gaagagcttt ttgatttgat tgtaaaattt gctggatatg
     3121 gatttaataa atctcactca gcagcatatg ctctaataac attctataca tcatacttaa
     3181 aatgttacta tccagcagaa tttatggctg ctttattaac tttggaaaaa gataatacag
     3241 ataaagttgt aaggtatgtt gatgaagtaa aaagattagg acttgagcta tttgctcctg
     3301 atataaataa atctgattta gtattttcag caagagttgt agataataga gaagttgtta
     3361 tgtttggaat gggcgcaatt aaaggtgctg gtgatgttgc tataaactca atattaaatg
     3421 caagagaaga tggcgatttt aaaaatctat cagactttgt atcaagaata gatgctagta
     3481 aagttaataa aagagttata gaatctttag caaaagcagg agtttttgat agttttggat
     3541 actcaagaaa tgcacttcta agtcaaatag aaaaaatagt tgatggcgct tcaaaagctt
     3601 caaatgccaa aaaaatgtca tctggttctt tgtttggaga tagtacagag cttacaagca
     3661 tagaaattga acttgataat ttacctgaat att
//
LOCUS       DAOQAN010000042         3679 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_33475_length_3679_cov_2.890177, whole genome shotgun sequence.
ACCESSION   DAOQAN010000042 DAOQAN010000000
VERSION     DAOQAN010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 3679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3679
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33475_length_3679_cov_2.890177"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="PKO10_01190"
     CDS             complement(<1..339)
                     /locus_tag="PKO10_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNW65809.1"
                     /translation="MNYKEIVKEVLLTEAKELEIASSSISFDIENIVNLIINSKGKLI
                     VTGVGKSGLVGAKIAATLASTGTSSFFLHPTEAMHGDLGMIGKDDIVLAISYSGESEE
                     LIQILPHIKRF"
     gene            complement(350..2371)
                     /locus_tag="PKO10_01195"
     CDS             complement(350..2371)
                     /locus_tag="PKO10_01195"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009295182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HNW65810.1"
                     /translation="MEEINKEEISKNSEIKTTEIREESSTNTGSEKREFRPRNPRPPR
                     AQNSEQNQENRNNSEQNQEKRAPFKKKKPNNKPRFINKDIPTAGDGWTNDLKKAYMIN
                     EKIHKDRLNPHYKLNLNTNAKLRITPLGGLNEIGGNMMVVETENEAIIVDVGMSFPDG
                     DMHGVDILIPDFTYLREIKDKIVAVIITHGHEDHIGAMPYLFKEMQFPIYGSPLPLEM
                     IGSKFDEHKMREHRALFRAISKRTPIKIGNDFEIEWMHVTHSIIDSSCIAIKTAAGTM
                     IHTGDFKIDHTPYDGFPTDIHRLAHYGEEGVLVMTSDSTNSHSPGFTKTEKAVGPTFE
                     RIFATATGRVIMSTFSSNIHRVAQAIEKALKYGRKICVIGRSMEKNLDISMTLGYIKF
                     PKDQFIEAHEINKYTDKEIMIVTTGSQGESMSALYRMAIHEHRHVKIKPEDQIILSAK
                     AIPGNEGSVSEIINHLLKAGAKVAYQDFADIHVSGHAAQEEQKLMLRLVKPKFFLPVH
                     GEYNHALKHGETGVDCGVLERNVYVMADGEQIEVNPKYLKKVKTVKSGKVYIDNQLNH
                     KISDDVVIDRQTMAKEGIVMIVAQINENDRTLASKPKVATFGFISDKQDRFFTNEIEE
                     ILNTFLVNAKPGIFKNSRILEDELRKVVRKHCVRKYKKYPMIVPTIFVQ"
     gene            complement(2355..3152)
                     /gene="rsmA"
                     /locus_tag="PKO10_01200"
     CDS             complement(2355..3152)
                     /gene="rsmA"
                     /locus_tag="PKO10_01200"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HNW65811.1"
                     /translation="MEKVKAKKEFGQNFLKDSSILDKIIQSMPHNNNHIVEIGPGLGD
                     LTKNLVKYKDVTAYEVDTDLIGILKSKFANEIESKKFTLKHIDVLRAWDELKFLHDGK
                     YDLIANLPYYIATNIILRAFEDELCEHIIVMVQKEVAQKFTAKVNDKEYSSLGIMTEL
                     ISKESKILFDVPAEAFDPMPKVTSSVLYIKKDLSLKFDKDFNRFLKACFVQPRKKLSK
                     NLSSILDKSKILALYEELELNDNLRPHEVSSSLYSQMHTKVKNGRDK"
     gene            3223..>3679
                     /locus_tag="PKO10_01205"
     CDS             3223..>3679
                     /locus_tag="PKO10_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002847885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HNW65812.1"
                     /translation="MNNLTKRIIPCLDVNNGRVVKGVNFVGLKDAGDPVEIAKRYNDE
                     GADELTFLDISASVENRGTIVDIVKNVAKEVFIPLTVGGGIRNLDDIYALLNVGCDKI
                     SVNSSAVKTPNLIDDGAKRFGSQCIVVAIDVKKVSDGSYHVFVKGGREDT"
BASE COUNT         1144 a          614 c          515 g         1406 t
ORIGIN      
        1 aaatcttttt atatgtggaa gaatttgaat aagctcctcg ctctctccac tatacgaaat
       61 agccaaaact atatcatctt ttccaatcat tcccaaatca ccatgcatcg cttcagttgg
      121 atgaagaaaa aatgaactag ttcccgtact tgcaagtgtt gctgcaattt ttgctcctac
      181 aagcccagat tttccaactc ctgtgacaat aagtttacct tttgaattta taataagatt
      241 tacaatattt tcaatatcaa aagagataga gcttgaagca atttcaagct cttttgcttc
      301 agtaagtaaa acttctttta ctatctcttt gtagttcata aagaattcct tattgtacaa
      361 atatagttgg aacaatcatt ggatattttt tatattttct tacacaatgt tttcttacaa
      421 cttttctaag ttcatcttct aaaattctac tatttttaaa tattccaggt tttgcattaa
      481 caaggaaagt attcaaaatc tcttctatct catttgtaaa gaatctatct tgtttgtctg
      541 atataaaccc aaatgtggca acttttggtt ttgatgcaag agttctatca ttttcattaa
      601 tttgagcaac tatcataaca ataccctctt ttgccatagt ttgtctatct ataacaacat
      661 cgtcagatat tttatgattt aactgattat cgatataaac tttcccactt ttaactgttt
      721 ttactttttt aagatactta ggatttactt caatttgttc accatcagcc ataacataaa
      781 catttctctc taaaacacca caatcaacac cagtctcacc atgttttaaa gcatgattat
      841 actctccatg cactggcaag aagaatttag gcttaactag tcttagcata agtttttgct
      901 cttcttgagc agcatgtcca gatacgtgaa tatcagcaaa atcttgataa gcaacttttg
      961 ctccagcttt taaaagatgg ttaataatct cagaaacact tccttcattt cctggaattg
     1021 cttttgctga taaaattatt tgatcctcag gtttaatttt tacatgtcta tgctcatgaa
     1081 ttgccattct atataaagca ctcattgact caccttgact tcctgttgtt acaatcataa
     1141 tctctttatc agtatattta tttatttcat gagcttctat aaattgatct tttggaaatt
     1201 ttatatatcc taaagtcata gagatatcta agtttttctc catagaacga ccaattacac
     1261 agatttttct tccatatttt agagcttttt caatagcttg tgcaactcta tgaatatttg
     1321 aagagaatgt actcataata actctacctg ttgctgtagc aaatattctt tcaaaagttg
     1381 gaccaacagc tttttcagtt tttgtaaatc caggagagtg agagttagtt gaatcagaag
     1441 tcataactaa aacaccctct tctccataat gtgcaagtct atgaatatct gttgggaaac
     1501 catcatatgg agtatgatct attttaaaat ctcctgtatg aatcatagtt ccagcagcag
     1561 ttttaatagc gatacaagat gaatcaataa ttgagtgagt tacgtgcatc cactcaattt
     1621 caaaatcatt tccaatttta attggtgttc tttttgaaat agctctaaaa agtgctctgt
     1681 gttctctcat tttatgttca tcaaacttac ttccaatcat ctccaaagga agtggtgaac
     1741 cataaattgg aaattgcatc tctttaaata gatatggcat agctccaata tgatcttcat
     1801 gaccatgagt aataattact gcaacaattt tatcttttat ctctctaaga tatgtaaaat
     1861 ctgggattaa gatatcaact ccatgcatat caccatctgg aaaactcata ccaacatcta
     1921 caatgattgc ttcgttctca gtctctacaa ccatcatatt cccaccaatt tcatttaatc
     1981 caccaagtgg agtgattcta agttttgcat ttgtatttag atttaattta taatgtgggt
     2041 ttaatctatc tttatgaatt ttttcattta tcatatatgc ttttttaaga tcattagtcc
     2101 aaccatcacc agctgtaggt atatctttat ttataaatct tggtttattg tttggttttt
     2161 tcttcttaaa aggtgctctt ttttcttgat tttgttcgct attatttcta ttttcttgat
     2221 tttgttcaga attttgagct cttggaggtc taggatttct tggtctgaac tctctttttt
     2281 cagaacctgt attagttgat gactcttctc ttatttcagt tgtttttatt tcactatttt
     2341 ttgaaatttc ttctttattt atctcttcca ttttttacct ttgtatgcat ttggctatac
     2401 aaagatgaac taacttcatg aggtcttaag ttgtcgttta actctaactc ttcataaaga
     2461 gctaaaatct tagatttatc aagaattgag ctaagatttt ttgatagttt ttttcttggt
     2521 tgcacaaaac aagctttcaa aaacctatta aaatctttat caaattttag cgataaatct
     2581 ttttttatat aaagaacaga agatgtaact ttaggcattg ggtcgaatgc ctcagctggt
     2641 acatcaaaaa gtatttttga ctctttagag ataagttctg tcattatccc caaagatgaa
     2701 tactctttat catttacttt tgcagtaaat ttttgagcta cctctttttg aaccattaca
     2761 ataatgtgtt cacaaagttc atcttcaaaa gctcttaata taatatttgt tgcaatgtag
     2821 tatggtaagt ttgctattaa atcatattta ccatcatgta aaaatttcag ctcatcccaa
     2881 gctcttaaaa catctatgtg ctttagtgtg aattttttac tctctatttc atttgcaaat
     2941 ttcgacttta agataccaat taaatcggta tcaacctcat aagctgtcac atctttgtac
     3001 ttgactaaat ttttagtcaa atcacctaag ccaggcccga tttctacaat atgattattg
     3061 ttatggggca tcgattggat gattttatct aatattgaac tatcttttaa aaaattttgt
     3121 ccgaactctt tttttgcttt tactttttcc attaggcaag agtatatctt tttttaactt
     3181 acatttaggt aatatcttta actaattttt aaggaattac aattgaataa tctaacaaaa
     3241 agaataatcc catgcctaga tgtaaacaat ggaagagttg taaaaggcgt aaactttgta
     3301 ggtctaaaag atgcaggaga tcctgttgaa atagcaaaaa gatacaacga tgaaggtgca
     3361 gatgagttaa catttttaga tattagtgca agtgtagaaa atagaggaac tattgtagat
     3421 attgttaaaa atgttgctaa agaggtattt atcccactaa ctgttggtgg aggaatacga
     3481 aatttagatg atatttatgc tttattaaat gttggatgcg acaaaatttc tgtaaattca
     3541 agtgctgtta aaacaccaaa cttaatagat gatggagcaa aaagatttgg aagtcaatgt
     3601 attgttgtgg caattgatgt taaaaaagta tcagatggct catatcatgt ttttgttaaa
     3661 ggtgggagag aagatacag
//
LOCUS       DAOQAN010000043         3676 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_33513_length_3676_cov_2.597901, whole genome shotgun sequence.
ACCESSION   DAOQAN010000043 DAOQAN010000000
VERSION     DAOQAN010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 3676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3676
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33513_length_3676_cov_2.597901"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..887
                     /gene="glnA"
                     /locus_tag="PKO10_01210"
     CDS             <1..887
                     /gene="glnA"
                     /locus_tag="PKO10_01210"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HNW65813.1"
                     /translation="KGGYFPVAPIDNGVDIRAEMMQVLEQVGLEVTLGHHEVAQGQHE
                     IGIVFGDLIEASDNVQKLKYVIKMVAHLNGKSATFMPKPLYGDNGSGMHVHQSIWKNG
                     KNLFYKQGEYGNLSDIARWYIGGIFKHARALAAFTNPSTNSYKRLIPGFEAPSILTYS
                     SQNRSASCRIPYGAGEKAVRVEMRFPDSTSCPYLAFSAMLMAGLDGIKNKYEPIGPMD
                     DDLFELSLDEIRERDIPQMPHTLRGSLESLIRNNDFLRPVFTKKMIDTYQHYKFSTQV
                     WPYEARPTPFEFKTMYSC"
     gene            1068..1538
                     /gene="ribH"
                     /locus_tag="PKO10_01215"
     CDS             1068..1538
                     /gene="ribH"
                     /locus_tag="PKO10_01215"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HNW65814.1"
                     /translation="MRVIEGSLRLKGNEKVAIINGRFNHIITDRLVEGAKDSFKRHGG
                     NEENLDLILVPGAFEIPFALEKALSSGKYDAVCCVGAVIRGATPHFDYISAEATKGIA
                     TVALKYGKPVSNGVLTTDTIEQAIERAGSKVGNKGAEAMVTIIEMLDLYSEMGK"
     gene            1540..1938
                     /gene="nusB"
                     /locus_tag="PKO10_01220"
     CDS             1540..1938
                     /gene="nusB"
                     /locus_tag="PKO10_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013459178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HNW65815.1"
                     /translation="MATRTQARESVIGLLYAYDLGNEEIAKFVDDILEEKKIRNSQKE
                     FALNLFNGTIKHISKIDEEIIKHLTQGTIEDVGSVEKSILRLAIYEILFENLSRAIVI
                     NEAIELSKKLASDGAPKFINAILDQIKIEK"
     gene            1950..2648
                     /gene="pyrF"
                     /locus_tag="PKO10_01225"
     CDS             1950..2648
                     /gene="pyrF"
                     /locus_tag="PKO10_01225"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HNW65816.1"
                     /translation="MKTNMKLCVSLDLESSKENLALVEKIKDFDIWLKVGFRTYLRDG
                     KKFLEDLKSINPNFKIFLDLKLYDIPNTMADAAEDISNFGLVDMFNVHASAGKFAMQT
                     VMGRIKNIPNRPLVLAVTALTSFDNSSFKEIYGKDIDTKAREFAKDTFEAGLDGVVCS
                     AFESIDIKNNTNKEFITLCPGIRPFGEDSGDQKRVADINFSKENLVDFIVVGRPIYKN
                     ENPNDIVKKILQNI"
     gene            2729..2819
                     /locus_tag="PKO10_01230"
     tRNA            2729..2819
                     /locus_tag="PKO10_01230"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2764..2766,aa:Ser,seq:gct)
     gene            2822..2898
                     /locus_tag="PKO10_01235"
     tRNA            2822..2898
                     /locus_tag="PKO10_01235"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2856..2858,aa:Ile,seq:cat)
     gene            complement(3187..>3676)
                     /locus_tag="PKO10_01240"
     CDS             complement(3187..>3676)
                     /locus_tag="PKO10_01240"
                     /inference="COORDINATES: protein motif:HMM:NF023540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative metalloprotease CJM1_0395 family
                     protein"
                     /protein_id="HNW65817.1"
                     /translation="KSKYEENQEKKENPTKLTSDEKAEVFKLKAIDSKIKAHEMAHKS
                     GPAASGGASFSYTKGPDGLMYAIAGEVPVEIKTGDTPQETISNMHDVIATALAPSDPS
                     PQDLSIASKARVIMMKAQQEFTKEIHEQITNSNEYTKNAKSEYEKNSNMQSLDKNSNI
                     KI"
BASE COUNT         1330 a          490 c          634 g         1222 t
ORIGIN      
        1 ttaaaggtgg atattttcca gtagctccta ttgataatgg tgttgatata agagctgaaa
       61 tgatgcaagt tttagagcaa gttggtcttg aagtaacact aggtcaccac gaagttgctc
      121 aagggcaaca cgagattggt attgtttttg gagatttaat tgaagctagt gataatgttc
      181 aaaaattaaa atatgttatt aaaatggttg ctcacttaaa tggaaaatct gcaacattta
      241 tgccaaaacc actttatggt gataatggaa gcggaatgca tgttcatcaa agtatctgga
      301 aaaatggaaa aaatctattc tacaaacagg gtgaatatgg aaatttaagt gatattgcaa
      361 gatggtatat tggtggaatt tttaaacacg cacgagcgct tgctgcattt acaaatccat
      421 ctacaaactc ttacaaaaga ttaattcctg gatttgaagc accttcaatt ttaacttatt
      481 cttcacaaaa tagatcagca tcttgtagaa ttccttatgg agctggtgag aaagcagtta
      541 gagttgaaat gagattccca gattcaactt cttgtccata tttagcattt tcagctatgt
      601 taatggctgg acttgatgga attaaaaata aatatgaacc aattggtcct atggatgatg
      661 atctatttga gttatcttta gatgagatta gagaaagaga tattcctcaa atgcctcata
      721 ctttaagagg ttctttagaa tctcttatta gaaacaatga ttttttaaga ccagttttca
      781 ctaaaaaaat gattgatact tatcaacact acaaattttc aactcaagtt tggccttacg
      841 aagcaagacc tacaccattt gagttcaaaa ctatgtactc ttgctaaatt tcttgagata
      901 agagttttac tcttatctca atttttatta cttttactaa atctataccc tttttaataa
      961 attcaataat caaaatatct tataaatagt tcataaacta ttgctttcag aaattaagta
     1021 ctatttttgt ataatgtagg ttatttataa agattttgga gtttaaaatg agagtaatag
     1081 aaggaagttt gagactaaaa ggaaatgaaa aagtagccat cataaatgga agatttaacc
     1141 acataataac tgatagatta gtagaaggtg caaaagattc attcaaaaga catggtggaa
     1201 atgaagaaaa tttagattta attttagttc caggagcttt tgaaatacca tttgctcttg
     1261 aaaaagcact atcaagtgga aaatatgatg ctgtttgttg tgttggagca gttataagag
     1321 gagcaactcc tcattttgac tatattagtg cagaagcaac aaaaggaata gcaacagttg
     1381 ctttaaaata tggaaaacct gtatcaaatg gtgttttaac aacagatacg attgagcaag
     1441 caattgaaag agctggttca aaagttggaa acaaaggtgc ggaagctatg gttacaataa
     1501 tagagatgct agatttatat agcgaaatgg gaaaataatt tggcaacaag aacacaagca
     1561 agagagtctg taattggact tctatatgca tatgatttag gtaatgaaga aatagcaaaa
     1621 tttgttgatg atattttaga agaaaaaaag ataagaaata gtcaaaaaga gttcgcttta
     1681 aatctattta atggaactat caaacatatt tcaaaaattg atgaagagat tataaaacat
     1741 cttactcaag gtacaataga agatgtagga agtgttgaaa aatcaatatt aagacttgca
     1801 atatatgaga tattatttga gaacttatca agagcaatag ttataaatga agcaattgaa
     1861 ctatcaaaaa aattagcaag tgatggtgct ccaaagttta taaacgctat tttagaccaa
     1921 ataaaaatag agaaataaaa ggtcaattta tgaaaacaaa tatgaaactt tgtgtatctt
     1981 tagatttaga aagcagtaaa gagaatcttg ctttagttga aaaaataaaa gattttgata
     2041 tttggttaaa agttggattt agaacatatt taagagatgg aaagaaattt ctagaagatt
     2101 taaagtctat aaatccaaac ttcaaaatat ttttggatct taaattgtat gatataccaa
     2161 atacaatggc agatgcagca gaagatattt caaactttgg acttgttgat atgtttaatg
     2221 ttcatgcaag tgcaggaaaa tttgcgatgc aaacagttat gggaagaata aaaaatattc
     2281 caaatagacc tttagtttta gctgttactg cccttacttc atttgataat agtagtttta
     2341 aagaaattta tggaaaagat atagatacaa aagctagaga gtttgcaaaa gatacatttg
     2401 aagcaggtct agatggtgtt gtttgttctg cttttgaaag tattgatata aaaaataata
     2461 ctaataaaga gttcattaca ttatgtcctg gaattcgtcc ttttggagaa gatagtggag
     2521 atcaaaaaag agttgctgat ataaactttt caaaagaaaa cttggtagat ttcattgttg
     2581 taggtcgtcc tatatataaa aatgaaaatc caaatgatat agtaaagaaa atattacaaa
     2641 atatataaaa aaacaaatgg ttaagggatt tttaagagat tttctcttat aattcccgtc
     2701 ctgattttat gaacttaaaa ttagaaacgg acagttgggt gagttggctg aaaccacctc
     2761 cctgctaagg agacgtactg gcaacggtac cgagggttca aatccctcac tgtccgccac
     2821 tgggtcgtta gctcagctgg ttagagcact cggctcataa ccgagtggtc gaaggttcga
     2881 gtccttcacg acccaccagc tattaatttc actaatttcc aaaaagtttc aaaactccct
     2941 ttttatagta ttttgtttgt tccattatat tctgtttact tacattaagt ttacttatag
     3001 gaaaaaaatt tgcggtacaa ttggcggtac aaaattaaaa tggaatttgt accataataa
     3061 tattgctaaa tacttaaaaa taatcgctta tacagaagta aaaagtttaa aacctaaaga
     3121 ttaaaagata agtgatgaaa acaataaact tgtatttaat atacataaaa taagtacttg
     3181 taagtactaa attttaatat tagagttctt atctaaagat tgcatattac tatttttttc
     3241 atactcactt tttgcatttt ttgtatattc atttgagtta gttatttgct catgtatctc
     3301 ttttgtaaac tcttgttgag ctttcatcat aataactcta gccttagaag caatagataa
     3361 atcctgtgga ctaggatctg aaggagctaa tgccgttgca ataacatcat gcatatttga
     3421 tattgtctct tgtggagtat ctcctgtttt tatctcaaca ggaacttctc cagctattgc
     3481 atacattaaa ccatcaggac cttttgtata actaaaagat gctccaccac ttgcagctgg
     3541 accactttta tgtgccattt catgtgcttt tatttttgaa tctatggctt taagtttaaa
     3601 aacttctgct ttctcatcac tggttaattt tgtaggattc tctttttttt cttgattttc
     3661 ttcatattta gatttt
//
LOCUS       DAOQAN010000044         3660 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_33749_length_3660_cov_2.618585, whole genome shotgun sequence.
ACCESSION   DAOQAN010000044 DAOQAN010000000
VERSION     DAOQAN010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 3660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3660
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33749_length_3660_cov_2.618585"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            132..1739
                     /locus_tag="PKO10_01245"
     CDS             132..1739
                     /locus_tag="PKO10_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014475529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine--lipid A transferase"
                     /protein_id="HNW65818.1"
                     /translation="MLRTSQNRLIVLSSIFLTLFYNYKFFKELILTYGFITSNIFYFL
                     SVTVVLTLLIIFLLTLFSSKYTTKPILITIFISSAFTAYFMDSYSVVIDSEMIRNSLQ
                     TSFKESVDLFSFRLVLYVVFLAIIPSYIVYKTKINYKSLKGEIVSKLKTITLSLVIIF
                     IIIFSFSKFYTSFFREHKPLRYNVNPIYWIYSIGNYVHKTLDTAPSEIKDIGLDSKVV
                     EDENEPKELIILVVGETARADRFSLNGYSKETNPLLKKEDLVSFTNMSSCGTSTAHSV
                     PCMFSIFPKDDYSYKKGASTKNVLDVLNNTQDVAILWRDNNSDSKGVALRVDYEDFQT
                     PKTNSICDEECRDEGMLVGLEDYIEKNRGRDILIVLHQMGNHGPAYYKRYPKEFEKFT
                     PVCKTNQLENCTQEEISNAYDNAILYTDYFLSKAINFLKKYSKTHEAGLLYVSDHGES
                     LGENGIYLHGMPYAIAPKEQTRVASLIWLDDGKMAHEYDKSQIKENRDKEYSHDNLFS
                     TILGLFEVKTEVYKKELDILNEARKED"
     gene            1717..2421
                     /locus_tag="PKO10_01250"
     CDS             1717..2421
                     /locus_tag="PKO10_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014469572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNW65819.1"
                     /translation="MKLEKKTKQIIFTAFLLWAVILIFQFTDFDIRFQSLFYNFETKS
                     WILSKDNYLADLIFYSGFKKIFIIFASIILLLSVLSFFKKIKILEKYKKGLLIVCLST
                     ILVPSLASLKSVTNVPCPVDIIEFGGDSIDVKILESYPKDYIQEKKQRCWPAGHATMG
                     FSLMSLYFLFKKPRNQKIALAFGVTIGVLTGGYKILIGDHFLSHTIVTMILAWLVILI
                     IHKLIIKENFEKSTKI"
     gene            2399..2761
                     /locus_tag="PKO10_01255"
     CDS             2399..2761
                     /locus_tag="PKO10_01255"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014469573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - diacylglycerol kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HNW65820.1"
                     /translation="MRNQPKYNFFKNTSYALKGLIDLIKNETSFKIELIVTIILIPVI
                     IFIDTNLTNKAILFITLMGMILAEAINSAIERVVDLVTLEHNPMAGRAKDVGSTIVFI
                     SISIFVITWVIILFDICQ"
     gene            2840..3241
                     /locus_tag="PKO10_01260"
     CDS             2840..3241
                     /locus_tag="PKO10_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014475532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1924 domain-containing protein"
                     /protein_id="HNW65821.1"
                     /translation="MKAFLIATLLATLSFSATVDDCLESLKQEVLKTDSSFKGFDYKR
                     GEEIFLSKHVGKKGELISCASCHGTDLNKSNQNYFTGKEIDALSPKVNPKRFTDKTEI
                     EKWLKRNFNDVYNREGTPLEKGDVITYIINK"
     gene            3257..>3660
                     /locus_tag="PKO10_01265"
     CDS             3257..>3660
                     /locus_tag="PKO10_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012148142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HNW65822.1"
                     /translation="MKKLILLGLTSSFLFAGGISVSVKPVDNTIYEKECGSCHFAYPA
                     GLLPSNSWNKMISNLSDHFGDDATVDEKTFQTISSYLNENSAEKSMQYKRSRKIVENL
                     NGTIPDSISKMPYMKKKHEDIKENLITQKEVKG"
BASE COUNT         1402 a          407 c          535 g         1316 t
ORIGIN      
        1 tattagatat ataattcaaa aataaaagta gcattttgct acttttaaat ctttacactt
       61 tcttgacatt ttattatgga tttattaatt ataaaaagat acaatttcac aaattatcgc
      121 aaggattttt cgtgttacga acttctcaaa ataggctaat agtacttagt tctatatttt
      181 tgactctatt ttacaattat aaatttttta aagaacttat tcttacatat ggatttataa
      241 cttctaatat tttctatttt ttatctgtaa ctgttgtttt aactctttta attatatttt
      301 tattaactct gtttagttca aaatatacaa caaaacctat tctaataaca atttttataa
      361 gttctgcatt tacagcatat tttatggata gttatagtgt tgttattgat tcagagatga
      421 ttagaaatag tttacaaaca agttttaaag agtcggttga tttatttagt tttagattag
      481 tcctttatgt tgtttttttg gcaattattc cctcttatat tgtatataaa actaaaataa
      541 attataaatc attaaaaggt gaaatagttt caaaattaaa aactataaca ttatctttag
      601 ttattatctt tataataatt tttagttttt ctaaattcta cacatctttt tttagagagc
      661 ataaacctct aagatacaat gtaaatccaa tttattggat atatagtatt ggaaattatg
      721 ttcataaaac tttagataca gcaccaagcg aaattaaaga tattggttta gattcaaaag
      781 tagttgaaga tgaaaatgaa cctaaagagt tgattatttt agttgttggt gagactgcaa
      841 gagcagatag attttcatta aatggttact ctaaagagac aaatccttta ttaaaaaaag
      901 aagaccttgt aagtttcaca aacatgtctt catgtggaac ttcaacagca cattcagttc
      961 cttgtatgtt ctctattttt ccaaaagatg actacagcta taaaaaaggt gcttctacaa
     1021 aaaatgtctt ggatgtttta aataatactc aagatgttgc aattttatgg agagataata
     1081 actctgattc aaaaggagta gcacttagag ttgattatga agattttcaa actcctaaaa
     1141 caaatagtat ttgcgatgaa gagtgtagag atgaaggaat gttagttggt cttgaagatt
     1201 atatagaaaa aaacagaggt agagatatat taatagttct tcaccaaatg ggaaatcacg
     1261 gtcctgcata ttataaaaga tatccaaaag agtttgaaaa atttacacct gtatgtaaaa
     1321 caaaccagct agaaaattgt acacaagaag agataagtaa tgcatatgat aatgcaattt
     1381 tatatactga ctatttttta tcaaaagcta taaatttctt aaaaaaatat tcaaaaactc
     1441 atgaggcggg attattgtat gtatctgatc atggagaaag tcttggagaa aatggaatat
     1501 atcttcatgg tatgccttat gcaatagctc caaaagagca aacaagagtt gcatctctta
     1561 tttggttaga tgatggaaaa atggctcatg agtatgataa aagtcaaatc aaagaaaata
     1621 gagataaaga gtactctcat gataacttat ttagtactat tttgggactt tttgaagtaa
     1681 aaacagaagt atataaaaaa gagttagata tattaaatga agctagaaaa gaagactaaa
     1741 caaattatat ttacagcttt tttactttgg gctgtaattt taatatttca atttacagat
     1801 tttgatataa gatttcaatc tcttttttat aattttgaaa caaaatcttg gattttatca
     1861 aaagataatt atttagcaga tttgattttt tatagtggat ttaaaaaaat atttataata
     1921 tttgcatcta ttattttact tctatcagtt ttgtcttttt ttaaaaaaat taaaatatta
     1981 gaaaagtaca aaaaaggttt attaatagtc tgcttaagca ctattttagt accttcttta
     2041 gcttctttaa aaagtgttac aaatgttcct tgtcctgtag atattataga atttggtggg
     2101 gattcaattg atgtaaaaat tcttgaatca tatccaaaag attatattca agaaaaaaaa
     2161 caaagatgtt ggccagctgg tcatgcaaca atgggtttct ctttgatgtc tctatatttt
     2221 ttatttaaaa aacctagaaa tcaaaaaata gctctagctt ttggtgtaac aataggtgta
     2281 ttaactggag gatataaaat attaattggt gaccactttt taagtcatac aatagttact
     2341 atgatattag cttggttagt aattttaatt attcataaat taataataaa ggaaaacttt
     2401 gagaaatcaa ccaaaatata acttttttaa aaatacatct tatgcattaa aaggtttaat
     2461 tgacttaata aaaaatgaaa catcatttaa aattgagcta atagtaacaa ttattctaat
     2521 tcctgtaatt atttttattg atacaaactt aacaaataaa gctattttat ttataacttt
     2581 aatgggaatg attttagctg aagcaataaa tagtgcaata gagagagttg tagatttagt
     2641 aactcttgaa cataatccta tggctggtcg agcaaaagat gtaggaagta ctattgtatt
     2701 tattagtata tctatttttg taattacttg ggtaattata ttatttgata tttgtcaata
     2761 aattgtcaag taaaaaaaca taatatcttt aaactattct tttacaattc taaatataaa
     2821 aaatatttag gagtaaatta tgaaagcatt tttaatagca actttacttg caactttaag
     2881 ttttagtgct actgttgatg attgtttaga gtccttaaaa caagaagttt taaaaacaga
     2941 ttcatctttt aaaggttttg attataaaag aggtgaagag atctttttat caaaacatgt
     3001 tggaaagaaa ggtgagttaa tctcttgtgc atcttgccat ggtacagatt taaataaatc
     3061 aaatcaaaat tattttacag gtaaagaaat agatgctctt tctccaaaag taaatccaaa
     3121 aagatttaca gataaaacag agattgaaaa atggttaaaa agaaacttca atgatgttta
     3181 taatcgtgaa ggaacaccgc ttgaaaaagg tgatgtaata acttacatca taaataagta
     3241 ataaaggagc aaaaagatga agaaattaat tcttttagga cttactagct ctttcttatt
     3301 tgctggagga attagcgtat ctgttaaacc agttgataat acaatttacg aaaaagagtg
     3361 tggtagttgt cattttgcat atccagcagg actacttcct agtaactctt ggaataaaat
     3421 gatctctaat ttaagtgacc actttggtga tgatgcaact gtagatgaaa aaacttttca
     3481 aactatttca agctatttaa atgagaatag tgctgaaaaa agtatgcaat ataaaagaag
     3541 tagaaaaata gttgagaatt taaatggcac tattcccgat tctatttcaa aaatgcctta
     3601 tatgaagaaa aaacatgaag atattaaaga gaatttaatt actcaaaaag aggttaaagg
//
LOCUS       DAOQAN010000045         3590 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_34724_length_3590_cov_3.514003, whole genome shotgun sequence.
ACCESSION   DAOQAN010000045 DAOQAN010000000
VERSION     DAOQAN010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3590
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34724_length_3590_cov_3.514003"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="PKO10_01270"
     CDS             <1..187
                     /locus_tag="PKO10_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine
                     deacetylase"
                     /protein_id="HNW65823.1"
                     /translation="ALLEYTMIGEYEAVAGSHHLNHLLTKKLYEDKENYEIIDLEEAS
                     SEAEVFELAFAKQLSAE"
     gene            192..644
                     /locus_tag="PKO10_01275"
     CDS             192..644
                     /locus_tag="PKO10_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014469340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW65824.1"
                     /translation="MIEILVITISKPLLIGVYKDKQLLRKIEQNGLTSDELPILFKDL
                     LEEFDVKTIIYVNTPGSFMSIKIAYIFLKTITMIKGIEFLAANGFLFNQNSPIKALGK
                     KYFINENEEIKVDFLPNMCKISDFELPKSLENIDFSKDTLPIYNLPAV"
     gene            909..1790
                     /gene="thrB"
                     /locus_tag="PKO10_01280"
     CDS             909..1790
                     /gene="thrB"
                     /locus_tag="PKO10_01280"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HNW65825.1"
                     /translation="MRISVPATSANLGPGFDCLGVALNLKNQVIIRPSKFHSVSLKGE
                     GSNNPALKDNNMFISIFNDFYYNLTQKKRHFRFEFFNEIPLSRGLGSSSAVIVSAIAS
                     AYAIEGIKVEKERLLNLSLAYESHPDNITPAVMGGFNVASVHENEVKFINKSIPRSLK
                     AVVVVPNRPISTTLSRKTLPYKYSKEDTIFNISHSSLLTAAFMSENWEMLKYASADMV
                     HQQFRMKHMPELFEVQKTALKNGALMSTLSGSGSTFFSIAYSEDSQKLELALKEKFPH
                     FRVMSVDFDNNGVKIEI"
     gene            1828..2094
                     /locus_tag="PKO10_01285"
     CDS             1828..2094
                     /locus_tag="PKO10_01285"
                     /inference="COORDINATES: protein motif:HMM:NF016204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF448 domain-containing protein"
                     /protein_id="HNW65826.1"
                     /translation="MANLKTILRTCIFCRKKLEQKELLRFKCSNKQLSIYDNIGRSFY
                     ICKPCKVDFLDMKDIKRYEKALCRECKNKDKYVIQLEEILLDVR"
     gene            2084..>3590
                     /locus_tag="PKO10_01290"
     CDS             2084..>3590
                     /locus_tag="PKO10_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HNW65827.1"
                     /translation="MSDKVRVFEIAEEAASTSAEVMQKAKELGIDLKTAQATVSYEDA
                     EEITQYIMTGKSSRIKEPVQEKVAKPKKEVEVKEESEVKVPKINEQDKVIGNSIKTEI
                     KKPTISKPILKTEENVTSLNDEDNMINPTKVVPKRKGLVIIKKKRPEEQEVKIVKEDF
                     EVKKTQKSLSDIFSENEEKNFEKNNRVREDNQRNQKVKKEKKKTPIKAQDHGRKIEVS
                     HTSEEFKSSDDSLLGDEVVLLDMDLQDNFKIFDEPKPVNTANHSRSSKPAAFGNTPTG
                     LKRGKRKKRVVRTQEKEDITSIVIPEDIRVYEFAEACGKTPAEVITVLFHLGMMVTKN
                     DFLKQDELEILGEEFGIEVTVKDALEDVNYEESYDEEIDTKSFVTRPPVVTIMGHVDH
                     GKTSLLDKIRSSKVASGEAGGITQHINSYTITKNGQKITFVDTPGHEAFSAMRTRGAN
                     VTDIIIIVVAADDGVKAQTEEVISHAKASGCPIIVAMNKMDKESANPDMVKA"
BASE COUNT         1430 a          408 c          643 g         1109 t
ORIGIN      
        1 ggcgctttta gagtatacaa tgattggtga gtatgaggct gttgctggaa gtcaccattt
       61 aaatcatctt ttgacaaaaa aactttatga agataaagaa aattatgaaa taattgattt
      121 agaagaggca agtagtgaag ctgaggtttt tgaacttgca tttgcaaaac agttgagtgc
      181 agagtaaaaa tttgatagaa attttagtta taactatttc aaaaccactc ttaattggtg
      241 tttataaaga taaacaactt ttaagaaaaa tagaacaaaa tggtttaaca tctgatgagt
      301 tgccaatttt atttaaagat ttattagaag agtttgatgt aaaaactata atttatgtaa
      361 atacacctgg ttcatttatg tctataaaaa ttgcttatat ttttttaaaa actataacta
      421 tgataaaagg tattgagttt ttagcagcta atggctttct ttttaatcaa aattctccaa
      481 taaaagcatt aggaaaaaaa tattttataa atgaaaatga agagattaaa gtagattttt
      541 tgccaaatat gtgtaaaatt tcggattttg aattaccaaa gagtttagaa aatatagatt
      601 tttctaaaga tactttgcca atatataatt taccggctgt ataaaggctt cattttgaag
      661 ctctttagga ttttctcaca gagagtttaa caacaggcta ctttttagga gatttttaat
      721 ctccgctata aaaagtagtt atagaagata aggttccttt ttaaatggga accatttaag
      781 acaggcttca ttcttgaagc actttaggtt tttgctactt tttaggagat ttttaatctc
      841 cgctataaaa agtagttata gaagataagg ttccttttta aatgggaacc atttaaaaag
      901 gagagtattt gagaattagt gttccagcaa ctagtgccaa tttagggcct ggatttgact
      961 gtttaggagt agctttaaat ttaaaaaatc aagtaataat tagaccatca aaatttcata
     1021 gtgtatcttt aaaaggtgag ggttcaaata atcctgcatt aaaagacaat aatatgttta
     1081 tatctatttt taatgatttt tactacaatt tgacgcaaaa aaagagacat tttagatttg
     1141 aattttttaa tgaaatccca ctttcaagag gtttaggaag ctcttcggct gttattgttt
     1201 ctgcaattgc tagtgcttat gcaattgaag gaataaaagt tgaaaaagag aggcttttaa
     1261 atctatcttt ggcatatgaa agtcatcctg ataatataac accagctgtt atgggtggat
     1321 ttaatgtagc aagtgtgcat gaaaatgaag ttaaatttat aaataaatca attcctagaa
     1381 gtttaaaagc agttgttgtt gtaccaaata gaccaatttc aactacacta tcaagaaaaa
     1441 cacttccata taaatattca aaagaggata caatttttaa tatatctcac tcatcacttt
     1501 taacagctgc atttatgagt gaaaattggg aaatgttaaa gtatgcatca gctgatatgg
     1561 ttcatcaaca atttagaatg aaacatatgc cagagctatt tgaagtacaa aaaacagctc
     1621 ttaaaaatgg cgctttaatg agtacacttt ctggttcagg ttcaacattt ttctcaatag
     1681 catatagtga agatagtcaa aagcttgaac tagctttaaa agagaaattt cctcacttta
     1741 gagtaatgag tgtagatttt gataacaatg gtgtaaaaat tgaaatttaa ttattttata
     1801 ttattaagaa aaatttaggt ataatagttg gctaatttga aaacaatctt aagaacttgc
     1861 atattttgta ggaaaaagct tgaacaaaaa gagcttttaa gatttaaatg ttcaaataaa
     1921 cagttatcta tttatgataa cattggaaga agcttttata tttgcaagcc atgtaaagtt
     1981 gattttctgg atatgaaaga tataaagaga tatgaaaaag cactttgtag agagtgtaaa
     2041 aataaagata agtatgttat acaacttgag gagattttat tagatgtcag ataaagtaag
     2101 agtttttgaa attgcagaag aagccgcatc aacaagtgct gaggttatgc aaaaagcaaa
     2161 agagttaggg atagatttaa aaacagcaca agctacagtt tcttatgaag atgcagaaga
     2221 gattacgcaa tatataatga ctggaaagag ttctagaatt aaagagccag tacaagaaaa
     2281 agttgcaaaa cctaaaaaag aggtagaagt aaaagaggaa tcggaagtaa aagttccaaa
     2341 aataaatgaa caagataaag taattggcaa ctcaataaaa actgaaatca aaaaaccaac
     2401 tatttcaaaa cctattttaa aaactgaaga aaatgttact agtctaaatg atgaagataa
     2461 tatgataaat ccaacaaaag ttgttccaaa aagaaaaggt cttgtaatta ttaagaaaaa
     2521 aagacctgaa gagcaagagg taaaaattgt aaaagaagat tttgaagtta agaaaactca
     2581 aaaatcttta agtgatattt ttagtgaaaa tgaagagaaa aactttgaaa agaataatag
     2641 agtaagagaa gataatcaaa gaaatcaaaa agtaaaaaaa gagaagaaaa aaactcctat
     2701 aaaagctcaa gatcatggaa gaaaaataga agtttcacat actagtgaag agtttaaaag
     2761 tagtgatgat tctcttttag gtgatgaagt tgttcttctt gatatggatt tacaagacaa
     2821 ttttaaaatc tttgatgagc caaaaccagt aaatactgca aatcactcta gaagttcaaa
     2881 acctgcagct tttggaaata ctccaacagg acttaaaaga ggaaaaagaa agaaaagagt
     2941 tgttagaact caagaaaaag aggatattac ttcgattgta attcctgagg atattagagt
     3001 ttatgagttt gcagaagctt gtggaaaaac tccagctgaa gttattactg tactatttca
     3061 cttaggaatg atggttacaa aaaatgactt tttaaaacaa gatgagttag aaatacttgg
     3121 tgaagagttt ggtattgaag taactgtaaa agatgcacta gaagatgtaa attatgaaga
     3181 gagttatgat gaggagattg atactaaatc atttgtaact agacctccag ttgtaacaat
     3241 catgggacat gttgatcatg gtaaaacatc tttattagat aaaattagaa gctcaaaagt
     3301 ggctagcggt gaagctggtg gaattacaca acatataaac tcatatacaa ttacaaaaaa
     3361 tggacaaaaa attacttttg ttgatactcc tggtcacgaa gcatttagtg caatgagaac
     3421 aagaggagca aatgttactg atattattat tatagttgtt gctgctgatg atggagtaaa
     3481 agctcaaaca gaagaggtaa tttctcatgc aaaagcaagt ggttgcccaa ttatagtagc
     3541 tatgaataaa atggataaag agtcggcaaa tccagatatg gtaaaagctc
//
LOCUS       DAOQAN010000046         3570 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_35008_length_3570_cov_3.448933, whole genome shotgun sequence.
ACCESSION   DAOQAN010000046 DAOQAN010000000
VERSION     DAOQAN010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 3570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3570
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35008_length_3570_cov_3.448933"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(120..1382)
                     /locus_tag="PKO10_01295"
     CDS             complement(120..1382)
                     /locus_tag="PKO10_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNW65828.1"
                     /translation="MESRSIYKQFYLKLIFATSLFLIILSFIFFEYTKNSFYDNIQDN
                     LLFQAKQIEKGYISFDKFQNVISSSQIIELIENNKNTTKQIEFSKFSKGDKSFIRLEF
                     LMHDNAILQITKDISLEKDILYLIIFKNFFALAIPGFIFMLIYALIVAKSLLKPIVQI
                     NKKLSNMDENSLSQIDKKDLPEEFLTLANSINSLTNRIGTYLKFKKELFIGIAHELKT
                     PLAVMKLKNELMLKKTREISQYEDAIRLTIKEIDGMNVMISSILDIGRTEGAQFEQAK
                     NIDLVEFIKYKTNDYNMLAAKKSITIKFSSNVSSLQMMIQETLFNQILQNFVQNAIKF
                     TPNGKTIKIKLKKIDDKVTLNVIDEGIGIDENIDVFAPFKKVGKENGVGLGLYLAKIA
                     SDALNAKISIKNREDGKNGAIAKLELNL"
     gene            complement(1400..2080)
                     /gene="hsrA"
                     /locus_tag="PKO10_01300"
     CDS             complement(1400..2080)
                     /gene="hsrA"
                     /locus_tag="PKO10_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homeostatic response regulator transcription
                     factor HsrA"
                     /protein_id="HNW65829.1"
                     /translation="MRILIIEDEITLNRTLQEGLIDFGYQVDTAENYKDAEYFIDIRN
                     YDLVLTDWMLPDGDGIELCKIVKNRSSRTAVVIISARDDKESEIEALKSGADDFIKKP
                     FDFDILLARIEARLRFGGTNIIEIDDLIINPDEEKIEYAGEEIELKGKPFEVLTHLAR
                     HRDQIVSKEQLLDAIWEEPELVTPNVIEVAINQIRQKMDKPLNISTIETIRRRGYRFC
                     YPNQVEEK"
     gene            2274..3086
                     /locus_tag="PKO10_01305"
     CDS             2274..3086
                     /locus_tag="PKO10_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNW65830.1"
                     /translation="MKKIVMSFIASIALISTLNAADFYATVDGDKITKDDINVLLQDP
                     RVDFDKLPQEAKSQILEGAINRKLIAKKAIDDGIEKDPQYKEAIAKIKEDLALQVWQK
                     NEIDKIKFSDTEKRAFYDTNKSKFNIPETFEAFHILVNSEAEANAVIKDLEKAAVNSR
                     ETKFKELANSKSKDATAKNGGYLGKFAAEQMVPEFAMAVKALPKGGYSKSPTKTQFGY
                     HVIFVKEIFPAKQLTFDEVKDNINQAMIAEKFNKKIDELTKELRKDAKIVIK"
     gene            3096..>3570
                     /locus_tag="PKO10_01310"
     CDS             3096..>3570
                     /locus_tag="PKO10_01310"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HNW65831.1"
                     /translation="MGVLDVVNAGVLTGSEAKKLFAYAKKEGFAIPAVNVVGTDSINA
                     VLEAASKVKSPVIVQFSNGGAGFYAGKGLKTSDAAILGAISGANHVHTMAKAYGIPVI
                     LHTDHAAKKLLPWIDGLLDAGKEHFAKTGRPLFTSHMLDLSEESLEENIEICVEYF"
BASE COUNT         1297 a          497 c          470 g         1306 t
ORIGIN      
        1 ctgatttttt tgatatgaaa ggtttatggt catttaaacc aaatttacaa tttgccataa
       61 tttttcctac ttttatatat ttttataata ttaaatagat tatatattaa aattaaatat
      121 tacaaattta attcaagttt tgcaatagca ccatttttcc catcttctct attttttata
      181 gaaattttag catttagtgc atcacttgca atttttgcta aatatagtcc caaacctaca
      241 ccattttctt ttccaacttt tttaaatgga gcaaaaacat ctatattttc atctatacca
      301 ataccttcat caattacatt tagtgtaact ttatcatcta ttttttttag cttaattttt
      361 attgttttac catttggagt aaattttatt gcattttgta caaaattttg taaaatctga
      421 ttaaaaagcg tctcttgaat catcatttgt aaactagata catttgaact aaattttatt
      481 gtaatacttt ttttagctgc aagcatattg taatcatttg ttttatattt tataaactca
      541 actaaatcaa tattttttgc ttgttcaaat tgtgccccct ctgttcttcc aatatctaaa
      601 atagagctaa tcataacatt cataccatct atctctttaa ttgtaagcct aatcgcatct
      661 tcatattgag aaatttctct tgtttttttt aacattaact cattttttaa cttcataaca
      721 gcaagtggcg ttttaagctc atgagctatt ccaataaaaa gctctttttt aaactttaaa
      781 taagtcccta ttctatttgt aagcgagttt atagagtttg ccaaagttaa aaactcttct
      841 ggtaaatctt ttttatcaat ttgagatagt gaattttcat ccatatttga tagtttttta
      901 tttatttgaa ctattggttt taaaagtgat tttgcaacta ttaatgcata aattaacata
      961 aaaataaaac caggaattgc aagtgcaaaa aagtttttaa atattattaa ataaagaata
     1021 tctttttcca aacttatatc ttttgtaatt tgtaaaattg cattatcatg cattaaaaac
     1081 tctaatctaa taaaagattt atcacctttt gaaaattttg aaaattctat ctgttttgtt
     1141 gtgtttttat tattttctat taattcaata atttgagaag aggatattac attttggaac
     1201 ttatcaaaac ttatgtaacc tttttctatc tgttttgctt gaaagagcaa attgtcttga
     1261 atgttgtcat aaaatgagtt ttttgtatat tcaaaaaaaa taaaagagag aataataaga
     1321 aaaagagaag ttgcaaatat aagctttaga taaaactgct tataaatact tctactctcc
     1381 ataataaatc agctaaaaat tacttttctt ctacctgatt tgggtaacaa aatctataac
     1441 ctcttcttct aatagtctca atagttgaga tatttaaagg tttatccatt ttttgtctaa
     1501 tttgatttat tgcaacctca ataacatttg gagttacaag ctctggttct tcccaaatag
     1561 catcaagaag ttgctctttt gaaacaattt gatctctgtg acgtgcaagg tgagttaaaa
     1621 cttcaaaagg tttacctttt aactctatct cttcaccagc gtactctatt ttttcttcat
     1681 caggattaat aattaaatca tcaatctcta taatatttgt tccaccaaat cttaatcttg
     1741 cttcaattct tgcaagtaaa atatcaaaat caaatggttt tttaataaaa tcatccgctc
     1801 ctgatttaag tgcttcaatt tcactctctt tatcatctct agcagagata ataacaacag
     1861 cagttcttga acttctattt tttacaattt tgcaaagttc aattccatca ccatctggta
     1921 acatccaatc tgttaaaacc aaatcatagt ttctaatatc gataaaatac tcagcatctt
     1981 tatagttttc agcagtatca acttgataac caaaatctat taatccctct tgtagtgttc
     2041 tgtttaatgt aatttcatct tcaataatta aaattctcat aaaaatcctt aagttaaatt
     2101 taatcttaaa attgcttgca agtatatcac aaaattttca aaatttaaag cttttttaag
     2161 gtaaatgtta aaaatcgtaa atctttttta aaataattat aaaattatat aaaaataaac
     2221 tatttattaa ctcgaaatgg ataaaatgtc gcaatttaat tttaaggaaa aaaatgaaaa
     2281 aaatagttat gagctttata gcttcaattg ctttaatttc aacattaaat gcagctgatt
     2341 tttacgctac tgttgatggg gataaaatta caaaagatga tatcaatgtt cttttacaag
     2401 atccaagagt tgattttgac aaacttccac aagaagcaaa gtctcaaatt cttgaaggtg
     2461 caataaatag aaaactaatt gcaaaaaaag caatagatga tggtattgaa aaagatcctc
     2521 aatataaaga ggctatagca aaaatcaaag aagatttagc tcttcaagtt tggcaaaaaa
     2581 atgagattga taaaatcaaa tttagtgata cagaaaaaag agctttttat gatacaaaca
     2641 aatcaaaatt caatattcca gagacatttg aagcatttca tattttagta aatagtgaag
     2701 ctgaagcaaa cgctgttata aaagatttag aaaaagctgc tgtaaattct agagaaacaa
     2761 aatttaaaga gttagcaaat tcaaaatcaa aagatgcaac tgctaaaaat ggtggatatt
     2821 taggaaaatt tgctgctgaa caaatggttc cagagtttgc aatggctgta aaagctcttc
     2881 caaaaggtgg atattctaaa agtcctacaa aaactcaatt tggttaccat gtaatatttg
     2941 ttaaagagat tttccctgca aaacaattaa cttttgatga agtaaaagat aatataaatc
     3001 aagcaatgat agctgagaaa ttcaataaaa aaattgatga gctaacaaaa gagttaagaa
     3061 aagacgcaaa aatcgtaatt aaataaggag attagatggg tgttttagat gttgtaaatg
     3121 ctggagtatt aactggaagt gaagctaaaa aactttttgc ttatgcaaaa aaagagggtt
     3181 ttgcaattcc tgctgtaaat gttgttggaa cagattctat aaatgctgtt ttggaagctg
     3241 caagcaaagt taaatctcca gttatagtac aattttcaaa tggaggagct ggattttatg
     3301 caggaaaagg tttgaaaact agtgatgctg ctattttagg agcaattagt ggagcaaatc
     3361 atgttcacac tatggcaaaa gcttatggaa ttccagtaat tttacataca gatcatgctg
     3421 ctaaaaaact tcttccttgg attgatggtt tacttgatgc aggaaaagag cattttgcaa
     3481 aaacaggaag accactgttt acatctcata tgctagatct tagtgaagag agcttagaag
     3541 agaatattga aatttgtgtt gagtatttta
//
LOCUS       DAOQAN010000047         3565 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_35085_length_3565_cov_2.025926, whole genome shotgun sequence.
ACCESSION   DAOQAN010000047 DAOQAN010000000
VERSION     DAOQAN010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 3565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3565
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35085_length_3565_cov_2.025926"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(176..442)
                     /gene="rpsR"
                     /locus_tag="PKO10_01315"
     CDS             complement(176..442)
                     /gene="rpsR"
                     /locus_tag="PKO10_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014473094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HNW65832.1"
                     /translation="MAERRKYGKKYCKYTEMKVDFIDYKNTELLKLSMSERGKIMPRR
                     LTGNSKNSQEMVEKAIKRARHMALVPYIVDTKNITDSAYARSFY"
     gene            complement(460..951)
                     /gene="ssb"
                     /locus_tag="PKO10_01320"
     CDS             complement(460..951)
                     /gene="ssb"
                     /locus_tag="PKO10_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNW65833.1"
                     /translation="MYNKTIMVGNLTRDIELKYLPSGSAVAKSSIASSYRYKSQTGEQ
                     KEEVCFLEFNMFGRSAEVANQYLKKGSKVLLEGRLVLEQWTAQDGSSRSRHSLRVEEM
                     KMLDSKGSNEGSSYGNNSYNQVPKEQYNEPSYNPNQGAGRVAQSRVEQNIPEIDIDDE
                     IPF"
     gene            complement(975..1331)
                     /gene="rpsF"
                     /locus_tag="PKO10_01325"
     CDS             complement(975..1331)
                     /gene="rpsF"
                     /locus_tag="PKO10_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HNW65834.1"
                     /translation="MSKLKHYETMFILKPTLTEEETVAQLDGVKALFEKNGAEIVATE
                     NIGIKELAYEIEKCKRGYYYVIYFKAPANSIAEIERNYRNNEELIRFMFIKYETKKEI
                     SSWTKMSEEAIKRASK"
     gene            complement(1413..2192)
                     /locus_tag="PKO10_01330"
     CDS             complement(1413..2192)
                     /locus_tag="PKO10_01330"
                     /inference="COORDINATES: protein motif:HMM:NF017911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW65835.1"
                     /translation="MFSSNNVVLIKIDKKIPKKELDALVESANINKDSTLILACIGDA
                     DFKSMESSFTAKQNACAVRFFVLMPNEAIGFLQNEAKRLKIDYDENALSHLYFMHRQN
                     ISLSTNDLSKLALLNERVTTKLVDLHCFGVGSVNFDDFLHDLIASKDISNDLSLLLEE
                     GLNEVFILNQISSFIQQLFMISSHARIYAVVNPIEVLGYNPPKNIWEKKSKLAINIKP
                     KQFLEMLDYILNLELSIKTEKIDNINLYLQASLRKFIVLFR"
     gene            complement(2208..2393)
                     /locus_tag="PKO10_01335"
     CDS             complement(2208..2393)
                     /locus_tag="PKO10_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW65836.1"
                     /translation="MYKSEFDKYLKANKRFSSYMFYGQSTFLVEQYAYLVSKIIAKDE
                     EIEKLYFEEYNFKYAKD"
     gene            2463..2837
                     /gene="fliW"
                     /locus_tag="PKO10_01340"
     CDS             2463..2837
                     /gene="fliW"
                     /locus_tag="PKO10_01340"
                     /inference="COORDINATES: protein motif:HMM:NF014660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HNW65837.1"
                     /translation="MYKVVLPILGFESFSKIDVEKLDEFVSFLKFEDGSKISIININV
                     LNKVSFDFKIDDKALEALKIKSKDDFSTYFILVSQEPVEHSIINLVAPIFINEKEKLV
                     GQYVTSEKVEPYLASLNKCINL"
     gene            complement(2832..>3565)
                     /locus_tag="PKO10_01345"
     CDS             complement(2832..>3565)
                     /locus_tag="PKO10_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HNW65838.1"
                     /translation="LKNSLLENIEVEKSTASHQLIEECMLLANIEASKKVGALGIFRI
                     HEEPNFKAISKLIDDVNILGVKAKLKDNVHETISHIQEESNKLFLRDEIDELVIQSLT
                     QAKYSSKNLGHFGLGFKSYSHFTSPIRRYSDLVIHRILKTKKLPKNIDDICTHISEQE
                     RKIDKLVWDFEDRKYARWAKDNIGGEVKVKIVDMERAKGVCYENMIGLKVTIENYKGQ
                     KLFSKHKVKIVSSNLATKLIVAKLL"
BASE COUNT         1135 a          579 c          392 g         1459 t
ORIGIN      
        1 catgttcttt atacttttca tgccaaatat tttctttgcc ttcataagta ttataatatt
       61 caaatcgtaa tcgtaatgct gtttttaatt gattttccat aaatttctct ttttaataga
      121 aataaaaaaa gggaagaagt aaaacttctt ccctttttat ttaccaaaat taatcttagt
      181 aaaatgatct tgcgtatgca gaatcagtaa tgtttttagt atcaacaatg taaggaacta
      241 atgccatgtg tctagctctt ttgattgctt tttctaccat ttcttgtgaa tttttagagt
      301 taccagtaag tcttctaggc ataattttac ctctttcact catagataat tttaataact
      361 cagtgttttt ataatcaatg aaatcaactt tcatctcagt atatttacaa tatttttttc
      421 cgtattttct tctttcagcc attgttaact ccttattttc taaaacggta tttcgtcgtc
      481 gatatcgatt tcaggaatat tttgctcaac tcttgattga gctactcttc ctgcgccttg
      541 attcggattg tatgaaggtt cattatattg ctcttttgga acttgattat aagagttgtt
      601 tccgtaagaa ctaccctcat ttgaaccttt actatccaac attttcatct cttcaactct
      661 taaagagtgt ctgcttctag aacttccatc ttgtgcagtc cactgttcaa gaaccaatct
      721 tccttctaat aaaactttag aacctttttt taagtattgg ttagcaactt cagcacttct
      781 tccaaacata ttaaactcta gaaaacaaac ttcttctttt tgctctcctg tttgcgattt
      841 atatctgtaa gatgaagcaa ttgaactttt agcaactgct gaaccactag gtagatattt
      901 taattcaata tctctagtta gattcccaac cataattgtt ttattataca taaaaggctc
      961 cttattttaa agcgttactt tgaagctctt tttatagctt cttcactcat tttagtccat
     1021 gaagaaatct cttttttagt ttcatattta ataaacataa atctaataag ctcttcattg
     1081 tttctataat ttctttcaat ctctgcgatt gaatttgctg gcgctttaaa atagattaca
     1141 taatagtaac ctcttttaca tttttcgatt tcgtatgcta actctttaat accaatattt
     1201 tcagttgcaa ctatctctgc accatttttt tcaaatagag ccttaactcc atcaagttgt
     1261 gctactgtct cttcttcagt taaagtaggc tttaaaataa acattgtttc ataatgtttt
     1321 aattttgaca ttcgttctcc ttatagattg tgccgatttt ctttacattc ccatttcatc
     1381 agcaaggata tttttttgta aagtttcgga ttttatctaa acaaaactat aaattttctt
     1441 aaacttgctt gtaaatataa atttatatta tcaattttct ctgtttttat actcaattcc
     1501 aaatttaaaa tataatctaa catctctaaa aattgtttgg gttttatatt tattgctagt
     1561 tttgattttt tttcccaaat attttttggg ggattataac ctaaaacttc aataggattt
     1621 acaactgcat aaattctagc atgtgaacta atcataaaaa gttgttgaat aaatgaacta
     1681 atttgattta aaataaaaac ctcatttaac ccctcttcta aaagaagtga taaatcattt
     1741 gatatatctt ttgaagctat caaatcgtgt aaaaaatcat caaaattgac acttcccaca
     1801 ccaaaacagt gtaaatctac taactttgta gttactcttt catttagaag tgctaattta
     1861 cttaaatcat ttgtactaag actaatattt tgcctatgca taaaatatag atggctcaaa
     1921 gcattttcat cataatcgat ttttaatctt tttgcctcat tttgtaaaaa acctatagct
     1981 tcatttggca ttaatacaaa aaatctaact gcacaagcat tttgtttagc agtaaaacta
     2041 ctctccatag atttaaaatc agcatctcca atacaagcta atataagtgt gctatctttg
     2101 tttatatttg cagattcaac aagagcatct aactcttttt ttggaatttt tttatctatt
     2161 tttatcaaaa ctacattgtt tgatgaaaac aaagatgatt gaagaagtca atctttggca
     2221 tatttaaaat tatactcttc aaaatatagt ttttctatct cttcatcttt tgctattatt
     2281 tttgagacta aataggcata ttgctcaacc aaaaacgttg attgaccata aaacatataa
     2341 gaactaaatc ttttgtttgc ttttaaatat ttatcaaatt cacttttata catggttaaa
     2401 atattaccct aagtttactt ttattataat ttttcttatt ttaattacat aaggggttta
     2461 tcatgtataa agttgttctt cctatcttag gatttgaaag tttttcaaaa atagatgtag
     2521 agaaattaga cgagttcgtt tcttttttaa agtttgaaga tggttctaaa atatctatca
     2581 taaacataaa tgttttaaac aaagtttcat ttgattttaa aatcgatgat aaagctttag
     2641 aggctttaaa aataaaatca aaagatgact tctctactta ttttatttta gtttctcaag
     2701 agccagttga acactcaatt attaacttag ttgccccgat tttcataaat gaaaaagaga
     2761 aactagttgg tcaatatgtt acaagcgaaa aagttgaacc atatttagca tctttaaaca
     2821 agtgtataaa cttataataa tttagcaact attaatttag ttgctaagtt gctagaaact
     2881 atttttactt tatgttttga aaatagtttt tgtcctttat aattttctat agttactttt
     2941 aatccaatca tattttcata acaaacaccc tttgctcttt ccatatcaac tatttttact
     3001 tttacttcac caccaatatt atcttttgcc catctagcat attttctatc ttcaaaatcc
     3061 caaacaagtt tatctatttt tctctcttgt tctgaaatat gagtacaaat atcatctata
     3121 ttttttggta attttttggt ttttaaaatt ctatgaatta ccaaatcaga gtatcttctt
     3181 ataggacttg taaagtgtga atatgattta aaacccaaac caaagtgccc taaatttttt
     3241 gagctatatt ttgcttgagt taaactttga ataacaagtt catctatctc atctcttaaa
     3301 aatagtttat ttgattcttc ttgaatatga gaaattgttt catgtacatt atcttttaat
     3361 tttgccttta ctcccaaaat attaacatca tcaattaatt ttgatatagc cttaaaattt
     3421 ggctcttcat gaattctaaa aattcccaaa gctccaactt ttttactagc ttcaatattt
     3481 gctaaaagca tacactcttc tattaattga tgagaagctg ttgatttttc tacttcaata
     3541 ttttctaata aactgttttt tagtt
//
LOCUS       DAOQAN010000048         3518 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_35749_length_3518_cov_2.682934, whole genome shotgun sequence.
ACCESSION   DAOQAN010000048 DAOQAN010000000
VERSION     DAOQAN010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 3518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3518
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35749_length_3518_cov_2.682934"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="PKO10_01350"
     CDS             complement(<1..577)
                     /locus_tag="PKO10_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014472837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNW65839.1"
                     /translation="MKYFLLFSILFFNILYASQNKEVLLIHSYHKGYAWTDDISKAID
                     KNFSEHKDIELTAVYMDTKRIDDSSYLEDLSKLYKQQFSNRKFDLVILSDNNAFDFVS
                     KYYDYLFKDAPILFCGINNYNKTILDKLPLKEVSGVAEEVDIEKNFELISKLHPNLKN
                     LLIINDKSITGLAVKKDLNPIISRTSRMVFAA"
     gene            complement(602..1189)
                     /locus_tag="PKO10_01355"
     CDS             complement(602..1189)
                     /locus_tag="PKO10_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HNW65840.1"
                     /translation="MTSKEKILNSIRENNIVKDVKLPSYENFGIKFDNKFETFSTMLE
                     SVGGKALVIEKEDLDKTIKELYPDEKIIASNVDFCSLGNFDSNSQDDTHNLKDIDLAI
                     VKGNFAVAENGAIWMKDESNRHRSLYFIAQNIVIVINENEIVNNMHEAYEKLSFEKAG
                     FGVFISGPSKTADIEQSLVIGAHGPKSGYVIFIKS"
     gene            complement(1182..2561)
                     /locus_tag="PKO10_01360"
     CDS             complement(1182..2561)
                     /locus_tag="PKO10_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012011953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein B"
                     /protein_id="HNW65841.1"
                     /translation="MSTNHNHSDNATKFVANDERMHWHDQALWFVREKRDRASKSIPE
                     WENLREYANQIKTHTMANLDKYLLEFEKNATEKGITVHFACDAKEHNEIVHKILSENN
                     VKKLVKSKSMLTEECHLNPYLESRGIEVIDTDLGERIVQLRHEPPSHIVLPAIHLKKS
                     DVSDTFHEKLGTEKGNYDPTYLTRAARAALREDFLTADAGLTGVNFAIAQTGGVVVCT
                     NEGNADMGASVPKLHIASMGIEKIIPRLEDLSVFTRLLARSATGQPITSYTSHFHGSV
                     EGGKMHVIIVDNKRSQFLSSQKNKKALNCIRCGACMNTCPVYRRSGGHSYEYVIPGPI
                     GSILGSARHPEAHNSLPFACTLCGSCTNVCPVKIDLDSQLYSLRQDLGEADLIDSKKK
                     MAMKVTTWLMSKPILFDFLGKTARFIVPKLPNFIVYNKANAWGKQRDLPKFPKKSFKE
                     MFKDGDLDD"
     gene            complement(2558..3307)
                     /locus_tag="PKO10_01365"
     CDS             complement(2558..3307)
                     /locus_tag="PKO10_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNW65842.1"
                     /translation="MKIGLFIPCFMNELYPDICKATYKLLKNQGLNIDYPLNQTCCGQ
                     PMANSGCAKDVKVLAINFVNNFKDYDYIVAPSGSCVTMVKEHYAEFFNDDKDYNKVKA
                     SIYEVCEFLHDIIKIENIKFDHSFPYKVGVHNSCHGHRVLKLATASELNIPYDSKLKN
                     LLNKVNNIELVTLKREDECCGFGGTFSVAEEAISVAMGKDRIKDHLDSNAQIITGADM
                     SCLMHMDGIINRDKNPIKVMHIVEILAGVRP"
BASE COUNT         1126 a          580 c          422 g         1390 t
ORIGIN      
        1 gcgctgcgaa aaccattcgt gaggtacgtg agattattgg atttaaatct ttttttacag
       61 ctaaaccagt tatagattta tcatttataa ttaaaagatt ttttaaattt ggatgtaatt
      121 ttgaaattag ttcaaagttc ttttctatat ctacctcttc tgcaactccc gaaacttctt
      181 taagaggtaa tttatctaat atcgttttat tatagttatt tattccacaa aatagaatag
      241 gagcatcttt gaataaatag tcataatatt tagatacaaa atcaaaagca ttattatcgc
      301 ttagtattac taaatcaaat ttacgatttg aaaattgttg tttatataat tttgatagat
      361 cctctaagta agatgagtca tctattcttt tggtatccat atatacagct gtaagctcaa
      421 tatctttatg ttctgaaaag tttttgtcaa ttgcttttga tatatcatct gtccatgcat
      481 atcctttatg atatgaatga attaataata cttctttatt ttgacttgca tataatatat
      541 taaaaaaaag tatgctaaat agtaaaaaat acttcattat ttctcctatt tagcaattat
      601 atcaagattt gataaaaatt acatatcctg attttggacc gtgtgctcca ataacaagcg
      661 attgttcaat atcagcagtt tttgaaggtc cagagataaa tacaccaaaa ccagcttttt
      721 caaaactaag tttttcataa gcttcatgca tattgtttac tatctcattt tcatttatta
      781 caataacaat attttgtgca ataaaatata aagacctatg tctatttgat tcatctttca
      841 tccaaattgc accattttct gcaactgcaa agttaccttt tacaatagct aaatcaatat
      901 cttttagatt atgagtatca tcttgtgaat ttgaatcaaa atttcctaaa ctacaaaaat
      961 ctacatttga agcaataatt ttttcatctg gatataactc ttttattgtt ttatctaaat
     1021 cttctttttc aataactaaa gctttaccac caacactctc aagcattgta gaaaatgttt
     1081 caaatttatt atcaaatttt attccaaaat tttcataaga aggaagtttt acatctttta
     1141 caatattatt ttctctaata gaatttaaaa ttttttcttt actagtcatc taaatctcca
     1201 tctttaaaca tctctttgaa acttttttta gggaattttg gtaagtctct ttgttttccc
     1261 cacgcatttg ctttattata aactataaaa tttggaagtt ttggaacaat aaatcttgca
     1321 gtttttccaa gaaaatcaaa taatataggt ttactcataa gccaagtggt tactttcata
     1381 gccattttct ttttgctatc aattaaatca gcttctccta aatcttgtct taaactataa
     1441 agttgtgaat ctaaatctat tttaactgga caaacatttg tacaagaccc gcataatgta
     1501 caggcaaatg gtaaactatt gtgagcttct ggatgtctag cacttcctaa aatagaacca
     1561 attggtcctg gtattacata ctcataagag tgaccaccac ttcttctata aactggacaa
     1621 gtattcatac aagcaccaca tctaatacaa tttaatgctt ttttattttt ttgagaactc
     1681 aaaaattgac ttcttttatt atcaacgata ataacatgca tctttccacc ttcaacactt
     1741 ccatgaaaat gtgaagtata tgaagtaatt ggttgaccag ttgcactacg tgctaacaat
     1801 cttgtaaata cacttaaatc ttcaagtcta ggaataattt tttcaattcc cattgaagca
     1861 atatgaagtt taggaacact agctcccata tctgcatttc cttcatttgt acaaactaca
     1921 accccacctg tttgtgcaat tgcaaaatta actcctgtta atcctgcatc tgctgttaaa
     1981 aagtcttctc taagtgcagc ccttgcagca cgtgtaagat atgttgggtc ataatttcct
     2041 ttttcagttc ccaatttttc atggaaagta tctgaaacat cagatttttt taagtgaatt
     2101 gctggaagaa ctatatgaga aggcggttcg tgtcttagtt gaactattct ttcacctaag
     2161 tctgtatcaa taacttcaat tcccctactt tctaaataag gattcaagtg acactcttct
     2221 gttaacattg attttgattt aacaagtttt tttacgttat tttcacttaa aattttatgt
     2281 actatttcat tgtgctcttt tgcatcacaa gcaaagtgaa ctgttattcc tttttctgtt
     2341 gcattttttt caaattctaa tagatattta tctaagtttg ccatcgtatg agttttgatt
     2401 tgatttgcat actctcgtaa attttcccat tcaggaatag atttactagc tctatctctt
     2461 ttttctctta caaaccataa tgcttggtca tgccaatgca ttctttcgtc atttgctaca
     2521 aattttgttg cattatctga gtggttgtga tttgtgctca tggtctaact cctgctagaa
     2581 tttcaactat atgcataact tttattggat ttttatctct atttatgatt ccatccatat
     2641 gcataagaca tgacatatca gcacctgtta ttatttgtgc attagaatct aaatggtctt
     2701 ttattctatc ttttcccatt gcaactgaaa ttgcttcttc tgcaacacta aatgtcccac
     2761 caaatccaca gcactcatct tctcttttta aagttactaa ttcaatgtta tttactttat
     2821 tcaaaagatt ttttaatttt gaatcatatg gaatatttaa ctcactagca gttgcaagtt
     2881 ttaaaactct atgtccatga catgaattat gtactccaac tttgtaaggg aaagagtggt
     2941 caaattttat attttcaatt tttataatat cgtgtaaaaa ttcacatact tcataaatag
     3001 aagcttttac tttattgtaa tctttgtcat cattaaaaaa ttctgcatag tgttctttta
     3061 ccatagtaac acatgaacca ctaggtgcaa caatataatc ataatctttg aaattattaa
     3121 caaaatttat tgctagaact tttacatctt ttgcacaacc agagtttgcc ataggttgtc
     3181 cacagcaagt ttgatttaat gggtaatcaa tatttaaacc ttgatttttt agtagtttat
     3241 atgtagcttt acaaatatcg ggataaagtt cattcataaa acatggtata aaaagaccaa
     3301 ttttcatatt ttctccttct tgttattaaa gtaattttat acataaaata tgaaaaactt
     3361 atgatatttt atttttttat gaagaaaaaa tcctttttat taagaaatag caggaatttg
     3421 ttctattttt actccattat cttctaaaaa ttttgagatg atttgagagt ttgtatgttc
     3481 ataaaatttc tcatatacta ttctttttat tccacttg
//
LOCUS       DAOQAN010000049         3508 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_35902_length_3508_cov_2.768317, whole genome shotgun sequence.
ACCESSION   DAOQAN010000049 DAOQAN010000000
VERSION     DAOQAN010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 3508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3508
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35902_length_3508_cov_2.768317"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="PKO10_01370"
     CDS             <1..245
                     /locus_tag="PKO10_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HNW65843.1"
                     /translation="KSMKYKRNRKIIENLNGVIPDSISKMPYMKKKHQDIKEHLITQK
                     EVKGLFNCTACHQNAKKGIFSDDDVKIPNYGRWEKN"
     gene            247..1032
                     /locus_tag="PKO10_01375"
     CDS             247..1032
                     /locus_tag="PKO10_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014475534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HNW65844.1"
                     /translation="MQKSYIWSLPTRVFHLLFALFILIAFLTDDDKLLKFHAIAGYSI
                     LILLFFRVYWGYFGPKYSLFRDFPANKSEAKEFFKNIFDGSFKNEQKYIGHNPLASYV
                     MIAMLIVTFIVIVTGVLAYGIQEGKGLASFLNDSFFKNMKLFKEIHEFFANFLIFLIV
                     AHLTGVAFDRVFHKKHETLNSIATGYKMTSEDESIKLSFFQKLFAIVMFVAFIAFLIF
                     NLYKPDNALVASKFEPIDYKTQNVAFVNECGSCHTLYPTKLIA"
     gene            1052..1375
                     /locus_tag="PKO10_01380"
     CDS             1052..1375
                     /locus_tag="PKO10_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013136929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HNW65845.1"
                     /translation="MGDLENHFGDDASLDEDVNKNILTFLLKNSAETSTMQASWNFLN
                     SIGDKDIIALSKTTYWERKHKKIPKEVFKNEKVKSVANCKACHSDIEKGLIENENIKD
                     ISDFM"
     gene            1341..1730
                     /locus_tag="PKO10_01385"
     CDS             1341..1730
                     /locus_tag="PKO10_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014469577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW65846.1"
                     /translation="MKILKIFLILCSIFLFLNGDDDYKKYKHSYKNLDYLNLDEKQVK
                     AIKNILLELKDEYKEFYEFKDDIEDDIEDLIEESNFDENLYIQKSMEIKKKATILEAK
                     RIKKILEILNEKQRDKFADHFKEWIIE"
     gene            1723..2388
                     /locus_tag="PKO10_01390"
     CDS             1723..2388
                     /locus_tag="PKO10_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013136931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNW65847.1"
                     /translation="MNKKVLLVEDDLQMQSLIVDYLKDYGFIVTAFDNPKDVLEDFKL
                     NSDYSIIILDLMLPFMDGFDLFNKLKEIKNIPIIISTARGDIGNKIHGFELGADDYLA
                     KPYEPRELVLRIESILKRNLTKSFKIGDFTIDKDNRTVLIDDYAIDFTKIEFEIFIYL
                     IENKNKISSREQILNATSLDFNTKNRTIDMHISNIRAKIGDDSKNPKYIKSVWGIGYK
                     FVA"
     gene            2441..>3508
                     /locus_tag="PKO10_01395"
     CDS             2441..>3508
                     /locus_tag="PKO10_01395"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HNW65848.1"
                     /translation="MTVIGLWIDNINLKRVDNFAKEKYLKVIDDIFKNIENKNYLDSL
                     ISKNSLEKVNFLDENNLETIYSQNSAFTDISILKYKSSNIYILKIKYLDESYIFKALD
                     EESFNDKTILNVLVFLDIFALLIMFLFIIKLLSPLKTITKELKAFANGNLSTRIDIKS
                     NDEIGTLAKSFNNMASSLENSIKTREELLRDIGHELRTPIAKGKFAIEKIDDFSQKEL
                     LKKIFQDLEKLTNELIELEKLNISKLNLTIFSAETLVLESLGKLYLEDESKVDIEIFE
                     DFKIEADLDYLSIALKNLIDNALKYAISFPIIIKIDKNQISILNSGEKLSKEFEYYLK
                     PFTQELIQRDGFGLGLSIVKKI"
BASE COUNT         1417 a          336 c          517 g         1238 t
ORIGIN      
        1 aaaaaagtat gaaatataaa agaaatagaa aaataattga gaatttaaat ggggttattc
       61 ccgattctat ttcaaaaatg ccatatatga aaaagaaaca tcaagatata aaagaacatt
      121 taattactca aaaagaggtt aaaggtttat ttaactgtac agcttgccat caaaatgcaa
      181 aaaaaggtat atttagtgat gatgatgtaa aaattccaaa ctatggaaga tgggagaaaa
      241 attaatatgc aaaaatcata tatttggtca ctgccaacaa gggtttttca tctcttgttt
      301 gctttgttta ttctaattgc ttttttaaca gacgatgata agcttctaaa atttcatgca
      361 attgctggtt actctatttt gattttatta ttttttagag tttattgggg atattttggt
      421 ccaaaatact ctttatttag agattttcca gcaaataaga gtgaagctaa agagtttttt
      481 aaaaatatct tcgatggctc ttttaaaaat gagcaaaaat acataggtca caatccatta
      541 gcttcttatg tgatgatagc tatgctaatt gttactttta ttgtaattgt tacaggtgta
      601 ttagcttatg gaatacaaga aggaaaggga ttagcatctt ttttaaatga ctcttttttt
      661 aaaaatatga aactttttaa agagattcat gaattctttg caaattttct tattttttta
      721 attgttgcac acttaactgg agttgccttt gatagagtgt ttcataaaaa gcatgaaact
      781 ctaaactcaa tagcaactgg atataaaatg acttctgaag atgagagtat aaaactatca
      841 ttttttcaaa aattatttgc tattgttatg tttgtagctt ttatagcttt tttgattttt
      901 aatctttata aaccagataa tgctttagtt gcttcaaaat ttgagccaat tgattataaa
      961 acacaaaatg tagcttttgt aaacgagtgt ggaagttgcc atactcttta tcccaccaaa
     1021 cttattgcct aaaaaatctt gggaattaat tatgggtgat ttggaaaatc attttggtga
     1081 tgatgcaagt ttagatgaag atgtaaataa aaatatttta acttttttat taaaaaatag
     1141 tgcagaaact tcaactatgc aagctagctg gaattttcta aattcaatag gagataaaga
     1201 tataattgct ttatcaaaaa ctacttattg ggaaagaaaa cacaaaaaaa ttccaaaaga
     1261 agtatttaaa aatgaaaaag taaaaagtgt tgcaaactgt aaagcttgtc atagtgatat
     1321 tgaaaaagga ttaattgaaa atgaaaatat taaagatatt tctgatttta tgtagtatat
     1381 ttttgttttt aaatggtgac gatgattaca aaaaatataa gcactcttac aagaatttag
     1441 attatttaaa tttagatgaa aagcaagtaa aagctataaa aaatattctt ttagagctaa
     1501 aagatgagta taaagagttt tatgaattta aagatgatat tgaagatgat attgaagatt
     1561 taatagaaga gtcaaatttt gatgagaatt tatatattca aaaaagtatg gaaattaaaa
     1621 aaaaggctac aattttagaa gctaaaagaa taaaaaagat attagaaatt ttaaatgaaa
     1681 agcaaagaga taaatttgca gatcatttca aggagtggat aattgaataa aaaggttttg
     1741 ttagttgaag atgatttaca aatgcaatct ttaatagttg attatctaaa agattatgga
     1801 tttatagtta ctgcttttga taatccaaaa gatgtattag aagattttaa actaaacagt
     1861 gactactcta ttataatttt agatttgatg cttccattta tggatggatt tgatctattt
     1921 aataagctaa aagagattaa aaatatacct ataattatct caactgcaag aggtgatatt
     1981 ggaaacaaaa tacatggatt tgagcttgga gctgatgatt atttagcaaa accttatgag
     2041 ccaagagagt tggttttaag aatagagtct attttaaaaa gaaacttaac taaatctttt
     2101 aaaattggtg attttacaat agataaagat aaccgaactg ttttaataga tgattatgca
     2161 attgatttta cgaaaataga gtttgaaatt tttatctatt taattgaaaa taaaaataaa
     2221 atatcttcaa gagagcagat tttaaatgct acttctttag attttaatac aaaaaacaga
     2281 actattgata tgcatatttc aaatattaga gcaaaaattg gtgatgattc aaaaaaccct
     2341 aaatatataa aatctgtttg gggaattggt tataagtttg tagcttagaa tgtcaatttt
     2401 caaaaaatta actatccttt ttattattag ttttatttta atgactgtaa ttggattgtg
     2461 gattgataat ataaatttaa aaagagttga taattttgca aaagagaaat acctaaaagt
     2521 tattgacgat atttttaaaa atatagaaaa taaaaattat ttagattctt tgatttcaaa
     2581 aaatagttta gaaaaagtaa attttttaga tgaaaataat ttagaaacta tatattctca
     2641 aaattctgca tttacagata tatctatttt gaaatataaa tcatcaaata tctacattct
     2701 aaaaataaaa tatttagatg agtcatatat tttcaaagca ttagatgaag agagttttaa
     2761 tgataaaact attttaaatg tactagtatt tttagatatt tttgcacttt taataatgtt
     2821 tttatttata attaaacttc taagtccact aaaaactata actaaagagt taaaagcttt
     2881 tgcaaatggt aatttatcaa caagaataga tataaaatcc aacgatgaaa ttggaacttt
     2941 agcaaagagt tttaataata tggcaagttc tttagaaaac tctataaaaa caagagagga
     3001 gttacttcgt gatattgggc atgagttaag aactccaata gcaaaaggaa aatttgctat
     3061 tgaaaaaata gatgattttt cacaaaaaga gctacttaaa aagatttttc aagatttaga
     3121 aaaacttaca aatgaactaa ttgagcttga aaaactaaat atttcaaaat taaatttaac
     3181 aatatttagt gcagaaactt tagtgttaga atctttggga aaactatatt tagaagatga
     3241 aagtaaagta gatatagaaa tttttgaaga ttttaagata gaagctgatt tagattatct
     3301 atcaattgca cttaaaaatc taattgataa cgctttaaaa tacgctattt catttccaat
     3361 aatcataaaa atagataaaa atcaaatctc tattttaaat agtggtgaaa aactatcaaa
     3421 agagtttgag tattatctaa aaccttttac tcaagaactt attcaaagag atggctttgg
     3481 tttaggttta agcattgtaa aaaagatt
//
LOCUS       DAOQAN010000050         3497 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_36062_length_3497_cov_2.540383, whole genome shotgun sequence.
ACCESSION   DAOQAN010000050 DAOQAN010000000
VERSION     DAOQAN010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 3497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3497
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36062_length_3497_cov_2.540383"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="PKO10_01400"
     CDS             complement(<1..366)
                     /locus_tag="PKO10_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002852612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HNW65849.1"
                     /translation="MSLWSQIKEDFSVPKLNDPAFNSNFEIFFNYPGVWAIINHRVAH
                     ILYKKGFEKLSRAISGISSFFTKTDIHPAATIGRRVFLDHAIGVVIGSTSIIEDDVLI
                     YQGVTLGGVSLNKGKRHPTI"
     gene            complement(389..2203)
                     /gene="speA"
                     /locus_tag="PKO10_01405"
     CDS             complement(389..2203)
                     /gene="speA"
                     /locus_tag="PKO10_01405"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HNW65850.1"
                     /translation="MKNRYGINNLSEYNFFIEDGVVKVNYKPYPSLIKIVKDVRKQGF
                     KGPLLLRFPHITKKQVKTLFNTFNASIKEYDYKGKFNAVFPLKVNQLPNFIHPLVSYG
                     KKYNYGLEAGSKAELIIAMTYNNMGSPITINGFKDKEMIHLCFIAKSMGHDITVIIEG
                     LNELEMILEVLKETKMEAPNVGLRVRLHSGGSGVWAKSGGIDSKFGLTSTEILEAFEL
                     MQENELEDLLTMIHFHIGSAMNTIKPLKKALRESGHIYAELKNLGAKKLNSINIGGGL
                     SVEYSAYEQSRFYSLAEFASDVVFTLKDIAKQKGVEEPNIFTESGRFISAASTVLITP
                     VLELFSAEYELDHLKLKETNPPLIEELRELFKDMTKKKAYEFMHDSIDHLESLLTLFD
                     LGYIDLQDRSNAEILTHQIIKKAISLLQVDDYEELKRFDKNIQEKYLLNFSLFQSLPD
                     YWGIDQEFPIMPITHLDKKPTRSASLWDITCDSDGEIPFDMKKPLYLHDVNLNKEDYF
                     LGFFNVGAYQDTLGMKHNLFSHPTEVNVVFKDEEVILEKLLESQKIIDILDDIDYDTQ
                     EMQTILSRNLPSETYEILKKYLDDNSYLKTIWSYYDDE"
     assembly_gap    2248..2257
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2258..>3497)
                     /gene="hisS"
                     /locus_tag="PKO10_01410"
     CDS             complement(<2258..>3497)
                     /gene="hisS"
                     /locus_tag="PKO10_01410"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014473929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HNW65851.1"
                     /translation="QSLRGMKDIVGSDSELFIYFVENASKIAQKYGFSYIETPLLEET
                     ALFKRSVGESSDIVNKEMYQFIDKGQNDVCLRPEGTAGVVRHFVEKKLDRAGGTYKWY
                     YYGPMFRYERPQKGRLREFHQFGCEVFGISNVYEDANIIMLIREILEYFGIGFTLKLN
                     SLGCVECMPQYKNNLVNHLNSFKSELCEDCNRRVDTNPIRVLDCKNEKCQTLLKDAPK
                     ITNSLCSSCDSDFEKLKEILDFNKINYEIDSNLVRGLDYYNKTAFEFVSNEIGAQSAI
                     AGGGRYDRLVEYLGGKSTAGIGFAIGIERLLELVKMNKEQKEFLYIGVLDEISLNKAF
                     EIAIKKRKTTKTYIEYTPRGFAKHFGIAEKLGCNIVALIGEKELENGIIYTKNLNTKE
                     ENNIIVRECIHSIISNNMPL"
BASE COUNT         1128 a          601 c          389 g         1369 t
ORIGIN      
        1 aattgtaggg tgtcgttttc ctttattaag actaactcca cctaaagtta caccttgata
       61 gattaaaaca tcatcttcta taatagaagt tgaaccaata acaactccaa ttgcatggtc
      121 taaaaatact cttcttccaa ttgttgcagc tggatgaata tctgtttttg taaaaaaaga
      181 tgatattcca gaaatagctc ttgaaagttt ttcaaaacct tttttatata aaatatgagc
      241 aactctatga tttatgattg cccaaactcc agggtaatta aaaaatatct caaagtttga
      301 gttaaaagct ggatcattta gttttggaac agaaaaatct tctttaattt gactccataa
      361 agatattttt tcttcttcgt tttttatctc attcgtcatc ataataactc catatagttt
      421 ttaaataact attatcatct aaatattttt ttaatatctc ataagtttca cttggaagat
      481 ttctacttaa aattgtttgc atctcttgtg tgtcatagtc aatatcatct aaaatatcta
      541 taattttttg agattctaaa agtttttcta aaattacctc ttcatcttta aatacaacat
      601 ttacttctgt tggatgggag aaaagattgt gtttcatccc taaagtatct tgatatgctc
      661 caacattgaa aaatcctaag aaataatcct ctttatttag atttacatca tgtaaatata
      721 gaggtttttt catatcaaaa ggaatttctc catcactatc acaagttata tcccataaac
      781 tagcacttct tgttggtttt ttatccaaat gagttattgg cataattgga aactcttggt
      841 caattcccca ataatcaggt aaagattgga aaagtgagaa atttagtaga tatttttctt
      901 gaatattttt atcaaatctt ttaagctctt cataatcatc aacttgaaga agtgaaatag
      961 cttttttgat aatttgatga gttaatattt cagcattaga tctatcttgt aaatcaatat
     1021 aacctaaatc aaaaagagtt aaaagtgatt ctaaatggtc tatactatcg tgcataaact
     1081 catatgcttt tttctttgtc atatctttga aaagttctct taactcttct attaaaggag
     1141 gatttgtctc ttttagtttt aagtgatcaa gttcatattc agctgaaaat agctctaaaa
     1201 caggagttat aagaacagtt gaagctgcac ttataaatct tcctgattct gtaaatatat
     1261 ttggctcttc aactcctttt tgttttgcaa tatcttttaa agtaaataca acatcacttg
     1321 caaattcagc aagagagtaa aatctagatt gttcgtaagc actatattct acacttaatc
     1381 ccccaccaat atttatagag ttaagttttt ttgcacctag atttttaagt tcagcataaa
     1441 tatgacctga ctctctaaga gcttttttca aaggtttaat agtattcata gcagaaccta
     1501 tatgaaaatg aatcattgtt aataaatctt caagttcatt ttcttgcata agctcaaaag
     1561 cttctaaaat ttctgttgaa gtaagtccaa atttagagtc aattcctcca ctttttgccc
     1621 aaacaccact tccaccgcta tgaagtctta ctcttaaacc tacatttgga gcttccattt
     1681 ttgtctcttt tagaacttct aaaatcatct ctagctcatt taaaccttca ataataactg
     1741 taatatcatg tcccatagat tttgcaataa agcatagatg tatcatctct ttatctttaa
     1801 aaccatttat agttattggg cttcccatat tgttgtaagt catagcaata attaattcag
     1861 ctttacttcc agcttcaaga ccataattat attttttccc gtaacttact aaaggatgaa
     1921 tgaagtttgg taactgattt acttttagag ggaaaacagc attaaatttt cctttataat
     1981 catactcttt tatacttgca ttgaaagtgt taaaaagtgt tttaacttgt tttttcgtaa
     2041 tatgagggaa tcttaaaagt aggggtcctt taaatccttg ttttcttaca tcttttacaa
     2101 ttttgattaa agatggatat ggtttgtagt ttacttttac aactccatct tcaataaaaa
     2161 aattatattc actcaaatta tttattccat atctgttttt aattttaaaa ttcttttaaa
     2221 attatattat tttcttcttt tttatttnnn nnnnnnnttc aatggcatgt tatttgatat
     2281 tatactgtga atgcactctc ttacaattat attattttct tcttttgtat ttaagttttt
     2341 tgtataaatt attccatttt ctagctcttt ttctccaatt agtgcaacaa tattacatcc
     2401 tagtttttca gctattccaa agtgctttgc aaaacctctt ggagtatatt caatataagt
     2461 ttttgttgtt tttctttttt taattgctat ttcaaaagct ttatttaaac taatttcatc
     2521 taaaacacca atatataaaa actctttttg ttctttattc atttttacaa gctctagcaa
     2581 tctttctatt ccaattgcaa aaccaattcc agctgtactc tttccaccaa gatattcaac
     2641 caatctatca tatcttccac caccagcaat tgcactttga gcaccaattt cattgcttac
     2701 aaactcaaat gcagttttgt tgtagtaatc aagcccacga accaaatttg agtcaatttc
     2761 ataatttatt ttattaaaat ccaaaatctc ttttaatttt tcaaaatcac tatcgcaaga
     2821 gctacataaa ctatttgtta tttttggagc atcttttaaa agagtttgac acttttcgtt
     2881 tttacaatct aatactctaa ttggatttgt atcaactctt ctattacaat cttcacacaa
     2941 ctcagattta aaactattta gatgatttac tagattattt ttatattgtg gcatacactc
     3001 aacgcaaccc aaagagttta gttttaaagt aaatccaata ccaaaatatt caagaatttc
     3061 acgaataagc ataataatat ttgcatcttc ataaacattt gatattccaa aaacttcaca
     3121 accaaattgg tgaaactctc ttaatcttcc tttttgtggt ctttcatatc taaacatagg
     3181 accatagtag taccacttgt aagttccacc agcacgatca agttttttct ctacaaaatg
     3241 tcttacaact ccagctgttc cttcaggtct tagacataca tcgttttgac ctttatcaat
     3301 aaattgatac atctctttat ttacaatatc gctactttct ccaacacttc ttttaaaaag
     3361 tgctgtctct tctaaaagag gagtctctat ataagaaaaa ccatattttt gtgctatttt
     3421 tgaggcattt tctacaaaat atataaaaag ttcactatca cttcctacaa tatccttcat
     3481 tcctcttaaa ctttgaa
//
LOCUS       DAOQAN010000051         3494 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_36104_length_3494_cov_3.266938, whole genome shotgun sequence.
ACCESSION   DAOQAN010000051 DAOQAN010000000
VERSION     DAOQAN010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 3494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3494
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36104_length_3494_cov_3.266938"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..832
                     /locus_tag="PKO10_01415"
     CDS             <1..832
                     /locus_tag="PKO10_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HNW65852.1"
                     /translation="GSAFTPAILNYIPQYTISLLLVIPFTILVIFFGTYYYYKVLKYD
                     LKTSYLGSMPGGVIEMVIIGQELKANTAQITLMQSSRLFFVVVSLPFIIQYIFQIDIR
                     GNQLLTTPIKDIIMFEFLYIYVLAVLAALFAKKIKLTAAYLIGPMIISIILYSGGFVT
                     THIPDELLKFIQVVFGSIIGFTFKNVDLKTIFNTLVATLGHFVILIILCGIFIFIIHH
                     FFGFATLDILLAFGPGGQTEINLIALLVGANLPYITLHHIVRLFLVMNIAPIIAKRLK
                     "
     gene            complement(854..1450)
                     /locus_tag="PKO10_01420"
     CDS             complement(854..1450)
                     /locus_tag="PKO10_01420"
                     /inference="COORDINATES: protein motif:HMM:NF015823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HNW65853.1"
                     /translation="MKQNKILFVSKSTFLVFLIFFIFSGCGLKQESLSINHYGIDFKT
                     QKKAEVSKLKSILIEEVNVNRSFNLTSIFYSTKPYLFEEYAQNRWINLPSNMIYNQLT
                     DSFITSNIFENVVLKDKKIEHEYSLKTEVIKLYQTFEEDKSYAVLKLKFDLVKDEKIV
                     KSFNFDKKVLCKSNNAYGFVQATNKAFEESINSLLSSF"
     gene            complement(1437..2357)
                     /locus_tag="PKO10_01425"
     CDS             complement(1437..2357)
                     /locus_tag="PKO10_01425"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNW65854.1"
                     /translation="MDTRINFFRIGLFVSVISFLLILAIFWLGKYGLEDKKYDTYYIY
                     FDESISGLNIGSPIKYKGFDVGLVKYIKISPHNSEKIEVEIQIQKGTPIKDDNYAILG
                     NLGITGLKYVELKGGSSEAKPLEENKYGLKIINSKKSALSNLVDSTEDITKELTFLLH
                     SFRDVLNEDNLKNFSILLENSQKSMANIEKFSDYLVKNQSNIDELLLSMKNFATIGSS
                     SFISVKTSADNFKALTTKMKEDFDKGNFDIKGMAQESINNLETVLKNLDNTLNQTQDL
                     MNNLNESPSDLIFKQKAIKYGPGEKDNETK"
     gene            complement(2357..3094)
                     /locus_tag="PKO10_01430"
     CDS             complement(2357..3094)
                     /locus_tag="PKO10_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014472952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNW65855.1"
                     /translation="MEIIKVSNLHTAFGDNIVHNDISFSVNEGEIFGVLGGSGSGKSV
                     LVKQIVMLNQIQKGKIEIFNKDISKLGIDDLESLKLNFSYLFQFGALYSFLNVIENIS
                     VMLKEYTNLPNDLIEKIAYTNLDIVGLPKHTAKLYPSELSGGMKKRVALARSLAMQPK
                     LLFLDEPTSGLDPASTQSVNELLLYLKKNLNITTVIITHDLETIKTVLDRFIIIRKEK
                     IFDGNITEAMSSKDEFIKEFLTHKKAD"
     gene            complement(3096..>3494)
                     /locus_tag="PKO10_01435"
     CDS             complement(3096..>3494)
                     /locus_tag="PKO10_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNW65856.1"
                     /translation="DAMRTMNFEPIIFLTIPRIFALCISLPLLVFFADIVGVMGGMVI
                     AYTSLDITYVEFINRLQNEVPVKHLLLGVFKALFFGFSIAIIGCFRGFQVENNTTSIG
                     KYTTISVVNAIFVVILLDAVFSVIFTQMGI"
BASE COUNT         1193 a          533 c          366 g         1402 t
ORIGIN      
        1 tggaagtgct tttactccag caattttaaa ctatattcca caatacacta ttagtttact
       61 tttagttatt ccatttacaa ttttagttat attttttgga acatactatt actataaagt
      121 tttaaaatat gatttaaaaa cctcttattt aggctcaatg ccaggtggtg ttattgagat
      181 ggttataatt ggacaagagc taaaagcaaa tacagcacag ataactttaa tgcaaagttc
      241 aagactattt tttgtagttg tatctttacc ttttattatt caatatattt ttcaaattga
      301 tataagaggt aaccagcttt taacaacacc aataaaagat ataattatgt ttgagttttt
      361 atatatctat gttttagcag ttttagcagc actgtttgca aaaaagataa aactaacagc
      421 tgcatatcta attggtccta tgattataag tattatttta tattcaggcg gatttgtaac
      481 tacacacata ccagatgagt tacttaaatt tattcaagtt gtttttggct caattattgg
      541 atttacattt aaaaatgttg atttaaaaac tatttttaat actttagttg ctactttggg
      601 acattttgtt attttaataa tcttgtgtgg aatttttatt tttattattc accacttttt
      661 tggttttgct actttagata tattacttgc ttttggacct ggtggtcaaa ctgagataaa
      721 tttaattgct cttttagttg gtgcaaattt accttatata actttgcacc atattgtaag
      781 actatttttg gttatgaata tcgcaccaat tattgctaaa aggttaaagt aattaaattt
      841 ttataaataa aaattaaaaa ctactcaata agctatttat actctcttca aaagctttat
      901 ttgtagcttg tacaaaacca taagcattgt tcgatttgca taatactttt ttatcaaaat
      961 taaaagattt tactatcttt tcatctttta ctaaatcaaa tttaagtttt aaaactgcat
     1021 aagatttatc ttcttcaaaa gtttgataaa gttttataac ttcagttttt aaactatatt
     1081 catgttcgat ttttttatct tttaaaacta cattttcaaa aatattactt gtaataaaag
     1141 aatctgtcaa ttgattataa atcatatttg aaggcaaatt tatccatcta ttttgtgcat
     1201 actcttcaaa taaatatggt tttgtactat agaaaataga tgttagatta aaacttctat
     1261 ttacatttac ctcttcaatt aaaattgatt ttaattttga aacctcagct tttttttgag
     1321 ttttaaaatc tattccataa tgatttatag ataaactctc ttgttttaat ccacatccac
     1381 taaatataaa aaatattaaa aaaactaaaa aagtactttt acttacaaat aagattttat
     1441 tttgtttcat tatctttttc tcctggtcca tattttattg ccttttgttt aaatatcaaa
     1501 tcacttgggc tttcatttag gttattcatt aagtcttgag tttgatttaa agtattatcc
     1561 aaatttttta aaactgtttc taaattatta atactctctt gagccatccc ttttatatca
     1621 aaatttcctt tgtcaaaatc ctctttcatc tttgttgtta atgctttaaa attatcagct
     1681 gaagttttta cactaataaa agatgagctt ccaatagtag caaagttttt catactaagt
     1741 aaaagttcat caatattact ttgatttttt actaaataat cagaaaactt ttcaatattt
     1801 gccatacttt tttggctatt ttcaagaagt attgagaagt tttttaaatt atcttcattt
     1861 aaaacatctc taaaagagtg aagtaaaaaa gtaagctctt ttgtaatatc ttcagtcgaa
     1921 tcaacaagat tactcaaagc tgattttttt gaatttatta tttttagacc atatttattt
     1981 tcttccaaag gttttgcttc actacttcca ccttttaact caacatattt aagtccagtt
     2041 atcccaagat tccccaaaat tgcatagtta tcatctttta ttggagttcc tttttgaatc
     2101 tgtatttcta cttcaatttt ttctgaatta tgaggactta tttttatata ctttacaagt
     2161 cctacatcaa aacctttata ttttataggt gaacctatat ttaatccaga aatagactca
     2221 tcaaaataga tataataagt atcatatttt ttatcttcta aaccatattt tcccaaccaa
     2281 aatattgcaa gaattagtaa aaaagatatt acacttacaa ataaacctat tctaaaaaaa
     2341 ttaattcttg tatccattaa tctgcctttt tatgtgttaa aaattctttt ataaactcat
     2401 ctttggaact catagcttca gttatattac catcaaatat cttctctttt cttataatta
     2461 taaacctatc aagaactgtt tttattgtct ctaaatcgtg agttattata actgttgtaa
     2521 tatttagatt tttctttaaa tatagtaaaa gctcatttac actttgtgta ctagcaggat
     2581 ctaatccact tgttggctca tccaaaaata gaagttttgg ttgcattgca agacttcgtg
     2641 caagggcaac tctttttttc attccaccac taagctcact tggatatagt tttgctgtat
     2701 gttttggaag acccactata tccaaatttg tgtaagcaat tttttctatc aaatcgtttg
     2761 gtaaatttgt gtactctttt aacatcacac taatattttc aataacattt aaaaaagagt
     2821 acaaagcacc aaattgaaaa agataagaaa aatttagctt caagctctct aaatcatcta
     2881 ttcctaattt tgaaatatct ttattaaaaa tctcaatttt acctttttgt atctgattta
     2941 gcataactat ttgttttacc aaaacactct ttccacttcc acttccacct aaaactccaa
     3001 atatttcacc ctcatttaca ctaaaagata tatcattgtg tacaatatta tctccaaaag
     3061 ctgtgtgaag atttgaaact tttattattt ccatcttata ttcccatttg agtaaatatt
     3121 acagagaaaa ctgcatctag taaaataaca acaaatattg catttacaac acttattgtt
     3181 gtatattttc caatacttgt agtattattt tcaacttgaa aacctctaaa acaaccaata
     3241 attgctatag aaaatccaaa aaatagagct ttaaaaaccc ctagtagtaa atgttttaca
     3301 ggaacttcat tttgaagcct atttataaac tctacatatg tgatatctaa acttgtatat
     3361 gctattacca ttccacccat tactccaaca atatctgcaa aaaataccaa aagtggcaaa
     3421 gatatacaca aagcaaaaat tctaggaatt gttaaaaata ttattggctc aaagttcata
     3481 gttctcatag catc
//
LOCUS       DAOQAN010000052         3485 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_36254_length_3485_cov_2.808746, whole genome shotgun sequence.
ACCESSION   DAOQAN010000052 DAOQAN010000000
VERSION     DAOQAN010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36254_length_3485_cov_2.808746"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="PKO10_01440"
     CDS             <1..367
                     /locus_tag="PKO10_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008242125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RimK/LysX family protein"
                     /protein_id="HNW65857.1"
                     /translation="LFDLDAKIDTGAYSNSLHCDDIFVDEDNFVHFKLLDKIHPSYHG
                     KKLKIPLYKTKSVKSSNGLVQTRASIKVQVKFAGKVYQTVVSLTNRSDMKYPMLIGRK
                     FLKDRFLVDVSKKNLTKGK"
     gene            369..1301
                     /gene="rimK"
                     /locus_tag="PKO10_01445"
     CDS             369..1301
                     /gene="rimK"
                     /locus_tag="PKO10_01445"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HNW65858.1"
                     /translation="MLVYILSRNENLYSTKRLFEEALNKGWEVRVIDYLKCTIEIMKN
                     ELVVNYEGEILKVPDAIIPRIGASRTFFGAAIVRHFEMQDVFSTTGNLALTRSRDKLR
                     SLQVLSKNGVDLPRTVFASNKSNAKDVIALSGGTPLVLKILEGTQGVGVVLVDSKKAA
                     KSVLDAFYGMDVNLLVQEYIEEASGSDIRVFVVNGEVVAAMKRQGSEGDFRSNLHQGG
                     SASVYKLNRKEKSIALAAAKSMGLGVCGVDMISSKRGPLVMEVNSSPGLEGIEKSTNI
                     NVALKIMDYIEQNIKPNCPTNPIKRKIKKDSIGA"
     gene            1391..1750
                     /locus_tag="PKO10_01450"
     CDS             1391..1750
                     /locus_tag="PKO10_01450"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNW65859.1"
                     /translation="MRKVIVFLIFTNFLFSYEELTVDNFDEKIKGKNAIIDFYASWCP
                     PCKILANNLEDFEIIKPDGVEIFKVNIEDQLVLAKKYGVKKLPTLIYFKDGKPIKEYV
                     GIQTSQELLDSSKKDFK"
     gene            1760..>3485
                     /gene="dsbD"
                     /locus_tag="PKO10_01455"
     CDS             1760..>3485
                     /gene="dsbD"
                     /locus_tag="PKO10_01455"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014469440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047134 - protein-disulfide reductase
                     (NAD(P)) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HNW65860.1"
                     /translation="MKKIVIFLMLFIYSFSLEIGNKVLEPEEAFKVDFIKNENSLNIK
                     IELGKDIYLYDDKLQVNITKPKKVELLKDLKLPKPVPYDGFIVHFDDLNIEIPYSLLK
                     SKLDSNKYEIEFKFQGCSKAGLCYAPMVEKQVIFFEADLKEEVKEDIKQKDEAISSLK
                     QDNENLSETDNIAATFKDSSFLLVVATFFGFGLLLAFTPCVFPMIPILSSIIVKASQN
                     ESMSAKKGFLMSFVYVLAMSFAYSLAGIIAGIFGANLQASLQNPYVLVVFAFVFIALA
                     FSMFGYFEIRLPASLQTKINKTTDGKEKQGMLGIAIMGFLSALIVGPCVAPPLAGALV
                     YIGQTGDALLGGVALFVMSLGMGVPLLLIGLGAGKFMPKPGGWMEGVTKIFGIIMLGV
                     AIWLLDRVINPTVTMYLWAFLFISFGVYLKIYTHIIVKSISKVLTILGAILLVGAVSG
                     ATNPLKPLDKFTSKVSLSKQDELKFIKIQNIAQLEDAILKSSKPIILDFWANWCVSCK
                     ELDEITFKNDEVIKRLQNFTLLKVDVTQNSEDDKAIQKKFGVVGPPALIFWDENKQEV
                     KSAKIVGYK"
BASE COUNT         1293 a          366 c          626 g         1200 t
ORIGIN      
        1 gctttttgat ttagatgcaa aaattgatac aggagcttac tcaaattctc ttcattgtga
       61 tgatatattt gtggacgaag ataactttgt acattttaaa ttattagata agattcatcc
      121 ttcataccat ggaaaaaaat taaaaatacc actgtataaa acaaagagtg taaaaagctc
      181 aaatggtctt gttcaaacta gagcatccat aaaagttcaa gtaaaatttg ctggaaaagt
      241 ctatcaaact gtagtttcat taacaaatcg ttctgatatg aaatatccta tgttaatagg
      301 aagaaagttt ttaaaagata gatttttggt tgatgtttca aaaaagaatt taacaaaagg
      361 aaaatagtat gttggtttat attttatcaa gaaatgaaaa tttatattcg acaaaaagat
      421 tatttgaaga ggctttaaat aagggttggg aagtacgagt tattgattat ttaaaatgta
      481 caatagagat tatgaaaaat gagcttgttg taaattatga gggggaaatt ttaaaagttc
      541 cagatgcaat aattccacga attggagcaa gtagaacatt ttttggtgca gcaatagtaa
      601 gacactttga gatgcaagat gtattctcaa cgactggaaa tttggcactt acaagaagta
      661 gagataagtt aagaagtctt caagttctat caaaaaatgg tgttgattta ccaagaacag
      721 tttttgcctc aaataaatca aatgcaaaag atgtaatagc gttaagtggt ggaacgcctt
      781 tggttttaaa aattttagaa ggaactcaag gtgttggagt tgttttagtt gatagtaaaa
      841 aagcagcaaa atctgttctt gatgcttttt atgggatgga tgtaaatttg cttgttcaag
      901 agtatattga agaggctagt ggaagcgata taagagtttt tgtggttaat ggtgaagtag
      961 ttgctgctat gaagagacaa ggttcagaag gtgattttag atcaaacctt catcaaggtg
     1021 gaagtgctag tgtatataaa ttaaatagaa aagagaaaag tatagctctt gctgctgcta
     1081 aatctatggg acttggagtt tgtggagttg atatgataag ttcaaaaaga ggacctttgg
     1141 ttatggaagt aaactcaagt cctgggttag aaggaattga aaaatcaaca aatataaatg
     1201 tagcattaaa aataatggac tatatagagc aaaatataaa accaaattgt ccaacaaatc
     1261 caataaaaag aaaaattaaa aaagatagta taggcgcata ataaaataaa tattataagt
     1321 aaatataata tttaatttaa actattcatt tactaatcta atatataatc taatttttta
     1381 aaggatatat atgcgaaaag ttatagtttt tttaatattt acaaactttt tattttctta
     1441 tgaagagtta acagttgata attttgatga aaagattaaa ggtaaaaatg caattataga
     1501 tttttatgca tcttggtgtc caccttgtaa gattttggca aataatttag aagatttcga
     1561 gattataaaa cctgatggtg ttgaaatttt taaagtaaat attgaagatc aattagtttt
     1621 agcaaaaaaa tatggtgtaa aaaagttacc aactttaatc tattttaaag atggaaaacc
     1681 aattaaagag tatgtaggaa tacaaacatc tcaagagctt ttagatagtt caaaaaaaga
     1741 ttttaaatag gggatttata tgaaaaagat agtaattttt ttaatgcttt ttatttactc
     1801 attttcttta gaaattggaa ataaagtttt agaaccagaa gaggcattta aagtagattt
     1861 tataaaaaat gaaaatagtt taaatataaa aatagagcta ggcaaagata tctatttata
     1921 tgatgataag cttcaagtta atattacaaa accaaaaaaa gtagaacttt taaaagattt
     1981 gaaacttcca aaaccagttc cttatgatgg atttattgtt cattttgatg atttaaatat
     2041 tgaaattcca tatagtttat taaaatcaaa actagattca aataagtatg aaattgagtt
     2101 taaattccaa ggttgttcaa aagcagggct ttgttatgct ccaatggttg aaaaacaagt
     2161 tatatttttt gaagctgatt taaaagaaga agttaaagaa gatataaaac aaaaagatga
     2221 agcaatatct tctttaaaac aagataacga aaatttaagt gaaacagata atatagctgc
     2281 aacctttaaa gatagtagtt ttttattagt agttgcaaca ttttttgggt ttggtctttt
     2341 attagccttt acaccttgtg tctttcctat gattccaatt ttgtcttcta ttattgttaa
     2401 agcttcacaa aacgagagta tgagtgctaa aaaaggcttc ttaatgtctt ttgtttatgt
     2461 tttagctatg agttttgcat attcattagc aggaattatt gcaggaattt ttggtgcaaa
     2521 tttgcaagct tctttacaaa acccttatgt attggttgtt tttgcatttg tatttattgc
     2581 tcttgctttt tcaatgtttg gatattttga aataagactt ccagcatctt tacaaacaaa
     2641 gataaataaa acaactgatg gtaaagagaa gcaaggaatg ttgggaattg caattatggg
     2701 atttttatcc gctttaattg ttggtccttg tgttgctcct cctcttgctg gtgctttagt
     2761 ttatatagga caaacaggag atgcacttct tggtggagtt gcgctttttg taatgagttt
     2821 aggaatggga gttccacttt tacttatagg attaggggct ggaaagttta tgccaaagcc
     2881 aggtggctgg atggaaggtg ttacaaaaat atttggaata attatgctag gtgttgctat
     2941 ttggctactt gatagagtaa ttaatccaac agtaactatg tatttatggg catttttatt
     3001 tatctctttt ggagtttatt taaagatata tactcatatt attgtaaaaa gtatttcaaa
     3061 agttttaacg attttaggag ctattttact tgttggtgct gtaagtggag caacaaatcc
     3121 tctaaaacca cttgataaat ttacaagtaa agtttccctt tcaaaacaag atgaattaaa
     3181 gtttattaaa attcaaaata ttgcacaact tgaggatgct attttaaaat cttcaaaacc
     3241 tataattcta gatttttggg caaattggtg tgtctcatgc aaagagttag atgagattac
     3301 ttttaaaaat gatgaggtta ttaaaagact tcaaaatttt acacttttaa aagtagatgt
     3361 tactcaaaat agtgaagatg ataaggcaat acaaaagaaa tttggagttg taggacctcc
     3421 tgcacttatt ttttgggatg aaaataaaca agaggtaaaa agtgcaaaaa tagttggata
     3481 taaaa
//
LOCUS       DAOQAN010000053         3469 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_36530_length_3469_cov_2.632103, whole genome shotgun sequence.
ACCESSION   DAOQAN010000053 DAOQAN010000000
VERSION     DAOQAN010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36530_length_3469_cov_2.632103"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="PKO10_01460"
     CDS             complement(<1..298)
                     /locus_tag="PKO10_01460"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNW65861.1"
                     /translation="MIKNFAKYIQNLPTLPEIIIDLDSFRKSKNRNFKQLAMIIKKDK
                     SLHLDILKIMDFQIFDFFNKPKNIQNFISITSLEFVCALATSLCFCRSIKTNLFS"
     regulatory      430..527
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            654..>925
                     /locus_tag="PKO10_01465"
     CDS             654..>925
                     /locus_tag="PKO10_01465"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013134058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HNW65862.1"
                     /translation="MQKWLEEHKNDKVRTQMYYAKQGIITPHMEYVAKVENLDAEFIR
                     SEIARGRLIIPANVNHTNQIPMAIGIASSCKINANIGSSALASDVS"
     assembly_gap    926..935
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <936..2043
                     /gene="thiC"
                     /locus_tag="PKO10_01470"
     CDS             <936..2043
                     /gene="thiC"
                     /locus_tag="PKO10_01470"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014472924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HNW65863.1"
                     /translation="VDCFINVVHRFEKVDVCLKYGADTIMDLSTGGDLDMIRKAVIAH
                     STVPIGTVPIYQILHDVKDKIEDLSIEKMLEVIERQAQQGVSYFTIHAGFLLEFMPHI
                     AKRKMGIVSRGGSLMAAWMMHYHKENPFNTAFDKILDICRKYDVSLSLGDSLRPGCLA
                     DASDEAQLRELKVLGDLTLRAWEKDVQVMIEGPGHVPLNQIERNMKIEKEYCHEAPFY
                     ILGPLTTDIAAGYDHISSAIGAAVGGWHGASMLCYVTPKEHLGLPNAEDVRNGIIAYK
                     IAAHSADIARGRKGARDIDDEMSDARYAFDWNRQFELCLDPDRAREYHDETLPQDVFK
                     EAEFCSMCGPKFCSYKITQKIVKEHGEAIDNMVG"
     gene            2255..3427
                     /locus_tag="PKO10_01475"
     CDS             2255..3427
                     /locus_tag="PKO10_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008245228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HNW65864.1"
                     /translation="MSIEDIKKALQSVKYPGFSKSIIDFGFVKDIKLDANSCTILLDI
                     TSTAKEVEDELRREIPKALPNLNINLVFNKPKEEQQKSNSVSGQNIAPQIKDIVMVSS
                     GKGGVGKSTTTVNLAIAAAMQGKKVGILDADIYGPNIPRMMGVNGKEVEVIGNKAKPL
                     NAYGVDIMSMGILMKEGEAVIWRGAMIMKAIQQLLRDILWEELDILFIDMPPGTGDAQ
                     LTLAQSVPVSAGINVTTPQHVALDDSRRSLDMFSKLHIPVAGIVENMSGFICPSCNTE
                     SDIFGTGTCDELAKQYNTQVLAHLPIEPAIRVGGDSGKPIVYFEPESISAKRYMMAAS
                     KLISMLENQGEVSNEAIQPIMPAGVSACSPEGQKIKAEHDAKKSSGGSCGTGCGCH"
BASE COUNT         1258 a          402 c          673 g         1126 t
ORIGIN      
        1 atgaaaagag atttgttttt atacttctac aaaagcataa agatgttgcc aatgcacaaa
       61 caaactctaa actagtaatt gagataaaat tttgtatatt ttttggttta ttaaaaaagt
      121 caaatatttg aaaatccata atttttaaaa tatctaaatg aagactctta tcctttttta
      181 taatcatagc aagttgttta aaatttctat tttttgattt tctaaaacta tctaaatcta
      241 ttatgatttc tggtaaagtt ggtaagtttt gtatatattt tgcaaaattt tttatcattt
      301 ttcctaaaaa cctaatatga aataaaaatg attatataaa attgtaactt atattatatc
      361 tactaaagga taattgagct ttaaaagaga attaatatgt ttttaatcat tctttatgct
      421 agaatagttt ttttattgcg gggggacgct ttggcgttga gaaggtaaaa cctgaccctt
      481 tgaacctgat ggttaagacc agcgtaggaa gtaaatttaa aaattaaatt tcatagttta
      541 aaatggataa aaattaataa aaagtacatt cttcggaaag tatttttttt ttgctcaaaa
      601 tttgtcttag caataaagaa aataatatta atttaggagc aaaaatgcaa aatatacaaa
      661 aatggttaga agaacataaa aacgataaag taagaactca aatgtattat gcaaaacaag
      721 gcattattac tcctcatatg gagtatgttg caaaagttga aaatcttgat gctgaattta
      781 taagaagtga aattgcacgt ggacgactta taattcctgc aaatgtaaat catacaaatc
      841 aaattcctat ggctattgga attgcaagtt cttgtaaaat aaatgcaaat attggaagtt
      901 cagcactagc aagtgatgtt agcaannnnn nnnnnggttg actgttttat aaatgtagtt
      961 catcgttttg agaaagttga tgtttgttta aagtatggtg ctgatactat tatggattta
     1021 agtacaggtg gagatttgga tatgattaga aaggctgtta tagctcactc tacagttcca
     1081 attggtacag ttcctattta tcaaatttta catgatgtaa aggataaaat agaggattta
     1141 agtattgaaa aaatgcttga agttattgaa agacaagcac aacaaggtgt gagttatttc
     1201 acaattcatg ctggattttt acttgagttt atgcctcata ttgcaaaaag aaagatggga
     1261 atagtttcaa gaggtggaag tttgatggca gcttggatga tgcactatca taaagagaat
     1321 ccatttaata cagcttttga taaaatatta gatatttgta gaaagtatga tgtatcttta
     1381 tctttgggtg atagtttaag accaggatgt ttagctgatg catctgatga agcacaacta
     1441 agagagctaa aagttcttgg agatttgact ttaagagctt gggaaaaaga tgtacaagtt
     1501 atgattgaag gacctggaca tgtgccttta aatcaaattg agcgaaatat gaagatagaa
     1561 aaagagtatt gtcatgaagc tcctttttat attttaggac ctcttacaac tgatattgct
     1621 gctgggtatg atcatatttc aagtgcaatt ggtgctgctg ttggtggatg gcatggagct
     1681 agtatgcttt gttatgttac tccaaaagag catttaggat taccaaatgc tgaagatgtt
     1741 agaaatggaa ttattgcata taaaattgca gctcatagtg ccgatatagc aagaggaaga
     1801 aaaggtgcaa gagatattga tgatgagatg agtgatgcaa gatatgcatt tgattggaat
     1861 agacagtttg aactttgttt agacccagat agagcaagag agtatcacga tgaaactttg
     1921 cctcaagatg tatttaaaga agcagagttt tgctcaatgt gtggaccaaa attttgttca
     1981 tacaagataa ctcaaaaaat tgtaaaagaa catggtgaag caatagataa tatggtgggg
     2041 taatttaaga caaaaattat cctaattaaa atataataac ttctcgattt atgaggaggt
     2101 tctttttttg tatgggaacc tatgatataa ggcttcgttc tcgaagccct ttagaatttt
     2161 gctactttta ggaatttttt ataaattccg ttaaaaagta gtttatatta gatggttcct
     2221 tttttatatg ggaacctata aaaaaggatt aaaaatgagt atagaagata ttaaaaaagc
     2281 tttacaaagt gttaaatacc caggtttttc aaaatcaatt attgattttg gatttgtaaa
     2341 agatattaaa ttagatgcaa actcttgcac aatactatta gatattactt caacagcaaa
     2401 agaggttgaa gatgagttaa gaagagagat tccaaaagct cttccaaatt taaatattaa
     2461 tttggttttt aataaaccaa aagaggagca acaaaaaagc aatagcgtaa gtggtcaaaa
     2521 tattgcacca caaataaaag atattgtaat ggtaagctca ggaaaaggtg gagttggaaa
     2581 atcaactaca actgtaaatc ttgcaattgc agcagctatg caaggtaaaa aagttggtat
     2641 tttagatgct gatatttatg gaccaaatat tccaagaatg atgggagtaa atggaaaaga
     2701 ggttgaagta ataggaaata aagcaaaacc tttaaatgct tatggcgtag atattatgtc
     2761 tatgggaatt ttaatgaaag agggagaggc tgttatttgg agaggtgcta tgattatgaa
     2821 ggcaatccaa cagcttttaa gagatatttt atgggaagag ttagatattt tatttattga
     2881 tatgcctcca ggaacaggag atgcacaatt aactcttgct caaagtgttc ctgtaagtgc
     2941 tggaataaat gtaacaactc ctcaacatgt agctcttgat gatagtagaa gatctttaga
     3001 tatgttctct aagcttcata ttccagttgc tggaatagtt gaaaatatga gtggatttat
     3061 atgtccatca tgtaacacag aatctgatat ttttggaaca ggaacatgtg atgagttagc
     3121 taaacagtac aatactcaag ttttagctca tcttccaatt gaacctgcta ttagagttgg
     3181 tggggatagt ggaaaaccaa tagtttattt tgaacctgaa tcaataagtg caaaaagata
     3241 tatgatggct gcaagtaaac ttatatctat gcttgaaaat caaggtgaag tttcaaatga
     3301 ggctattcag ccaattatgc cagcaggggt tagtgcttgt tctcctgaag gacaaaaaat
     3361 aaaagctgaa catgatgcta aaaaaagtag tggtggaagt tgcggaactg gatgtggctg
     3421 tcactaaaag aatgcttgga taggtagaat acctatccaa atttataat
//
LOCUS       DAOQAN010000054         3464 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_36604_length_3464_cov_2.682605, whole genome shotgun sequence.
ACCESSION   DAOQAN010000054 DAOQAN010000000
VERSION     DAOQAN010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36604_length_3464_cov_2.682605"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="PKO10_01480"
     CDS             complement(<1..392)
                     /locus_tag="PKO10_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HNW65865.1"
                     /translation="MNFTFNAYYTLIAAVIVLLIGKFLVNRIEFLRKYNIPEPVAGGL
                     VAATISTLVYNFWGYSITTSSELQTSFMLIFFISIGLSANFAKLKEGGKSLFIFLLVV
                     SGFIIIQNFVGISLATALGIEPLIGLIAG"
     gene            complement(414..803)
                     /gene="ruvX"
                     /locus_tag="PKO10_01485"
     CDS             complement(414..803)
                     /gene="ruvX"
                     /locus_tag="PKO10_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014473071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HNW65866.1"
                     /translation="MKLASIDIGLKRIGVAISLMNDIVTPQNAIIRKNRNQAALEVNN
                     FLKEWQIEKLIIGLPSSSEDTQNRIKHFTNLLELKIPYEFCEENLSSIEAKELMKGEV
                     KQVRDGRIDSIAAKIILERYINLIKKN"
     gene            complement(800..1714)
                     /locus_tag="PKO10_01490"
     CDS             complement(800..1714)
                     /locus_tag="PKO10_01490"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HNW65867.1"
                     /translation="MFETLISKLQEDKYLTLETTPQHEPSMHNIVEKIKKFKIDSKVD
                     GFTCTDNPLAKLKYNSLFAALKLQQEFKKPVIATMTMRDRNKIALQSDLLGANDFDIR
                     AILALTGDPANMSDQPNSKGVFEANSLMLLKMIKSFNYGMDFAGRPFKIEPKQIFPFA
                     VVNAYAKNFGSLEKKMHLKIQNGAVGVITQPVFDIENVKKLLENFENAKENVEGDKRK
                     AQLIFGVFPITKLRTALFLSAQVPGIHVPQFWIDELEKAHNISEEEEYKVGMQLSKNL
                     FDEINRIHPKIHLMTANNKFEVASEILA"
     gene            1795..2421
                     /gene="serB"
                     /locus_tag="PKO10_01495"
     CDS             1795..2421
                     /gene="serB"
                     /locus_tag="PKO10_01495"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HNW65868.1"
                     /translation="MKLAVFDFDSTLMDGETIDILAFEFGLGDEVKKITEEAMSGRLD
                     FFESLIQRVALLKGMEHKKVVEICANLPLMKGSYEIVKELKKMDYKVVCFSGGFRVGT
                     SPAREKLGLDADFSNILHEKDGILTGLVGGDMMFGYSKGDMIRRLQNLLKISKENTLV
                     CGDGANDLSMFAEANTRVAFCAKDVLKKEANIIVDTKDLTKILEHIKA"
     gene            2425..3417
                     /locus_tag="PKO10_01500"
     CDS             2425..3417
                     /locus_tag="PKO10_01500"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HNW65869.1"
                     /translation="MKEFEDINYSIWCDFIERDFLENRFQEIINDEVIKGATSNPAIF
                     EASITGSVAYKQQLDMLQANDQKKIYEELAFKDIRRAAELLEPLYKIDTNNGFISIEV
                     DPLLCDDAAGTIEEGLRIYKSLNADNVMIKIPATDAGYIAMRELSSRGIHINATLIFS
                     PQQARNCAIALDDGIKASNKDTKGVVSVFVSRFDRLMDTTLGAKGLATSKLGIVNATK
                     CYYEVNKLENSNIRTLFASTGVKGNELSPTYYIDNLIYPNSVNTAPLGTIEDWLKDGK
                     KEQSVIISEDECDKYFKSLEQNGIKLKDVYEKLLSEGLDAFKVSFKELLSKLKH"
BASE COUNT         1231 a          444 c          541 g         1248 t
ORIGIN      
        1 ccagcaatta gaccaattaa aggctcaatt cctaaagctg tagcaagaga tattcctaca
       61 aagttttgaa ttataataaa accagaaact acaagaagaa aaataaaaag agattttcct
      121 ccctctttta gttttgcaaa atttgcacta agaccaattg agataaaaaa tataagcata
      181 aaacttgttt gaagttcact actcgttgtt atactatatc cccaaaaatt atatactaaa
      241 gtagaaatag tagctgccac aagaccacca gcaacaggtt ctggaatatt atactttctt
      301 aaaaattcaa ttctatttac taaaaattta ccaataagta aaactattac tgctgcgatt
      361 aatgtatagt aggcattaaa tgtaaaattc ataaactttc ctttttcaaa tatttaattc
      421 ttttttatta aatttatata tctttctaaa attatttttg cagcaattga gtcaattctt
      481 ccatctctta cttgttttac ttcacctttc ataagctctt ttgcctctat tgagctaaga
      541 ttttcttcac aaaattcata aggaatttta agttcaagta aatttgtaaa gtgttttatt
      601 ctattttgag tatcttcact tgagcttgga agtccaatta ttagtttttc tatttgccac
      661 tcttttaaaa aattatttac ttcaagagca gcttgatttc tattttttct aataattgca
      721 ttttgtggag ttacaatatc attcataaga gaaatagcaa ctccaattct ttttaatcca
      781 atatctatag aagctaactt caagctaaaa tctcacttgc aacttcaaat ttgttatttg
      841 cagtcattag gtgaattttt gggtgaattc tatttatttc atcaaataaa tttttgctta
      901 actgcattcc tactttatac tcctcttcct cacttatgtt atgagctttt tccaattcat
      961 ctatccaaaa ttgaggtaca tgaatacctg gaacttgtgc agataaaaat agggcagttc
     1021 ttagttttgt aataggaaaa actccaaaaa ttaattgagc ttttctttta tcaccttcta
     1081 cattctcttt tgcattttca aaattttcta aaagtttttt tacattttca atatcaaaaa
     1141 caggttgagt aataacccca acagcaccgt tttgtatctt tagatgcatt tttttctcta
     1201 atgaaccaaa atttttggca taggcattta caactgcaaa tggaaaaatt tgtttaggct
     1261 caattttaaa agggcgacct gcaaaatcca ttccatagtt gaaagattta atcattttta
     1321 aaagcattaa agagtttgct tcaaaaactc ctttactatt tggttgatca ctcatatttg
     1381 ctggatctcc tgtaagtgct aaaattgctc ttatatcaaa atcatttgca ccaagcaaat
     1441 ctgattgtaa agctatttta tttctatctc tcattgtcat tgttgcaatt actggttttt
     1501 taaactcttg ttgaagtttt agtgctgcaa aaagtgagtt gtatttaagt ttcgctagtg
     1561 gattgtctgt acaagtaaaa ccatctactt ttgaatcaat tttaaatttt ttgattttct
     1621 caacaatatt atgcattgat ggttcatgct gaggtgttgt ttcaagtgtt aaatatttat
     1681 cctcttgaag tttagaaata agtgtttcaa acattttaaa tatattcctt ttaaactatt
     1741 ttagttaatt ttggttaata ttataccttt taatatttaa tatttaggat atttatgaaa
     1801 ttagcagttt ttgattttga ctcaacactt atggatgggg aaacaattga tattttagct
     1861 tttgagtttg gtttgggtga tgaagttaaa aaaattacag aagaggctat gagtggaaga
     1921 cttgattttt ttgaatcttt aattcaaaga gttgcacttt taaaaggaat ggaacacaaa
     1981 aaagttgtgg aaatttgtgc aaatttacct ttaatgaaag gttcttatga gattgtaaaa
     2041 gagttgaaaa agatggatta taaagttgtt tgtttcagtg gtggttttag agttggaaca
     2101 agcccagcaa gggaaaaact aggacttgat gctgattttt caaatatctt acatgaaaaa
     2161 gatggaattt taacaggact tgttggtgga gacatgatgt ttggatactc aaaaggagat
     2221 atgataagaa gattacaaaa tcttttaaaa atctcaaaag agaatacttt ggtttgtgga
     2281 gatggagcaa atgatttatc tatgtttgca gaagctaata caagagttgc tttttgtgca
     2341 aaagatgtac ttaaaaaaga ggcaaatatt atagtagata caaaagattt aacaaaaatt
     2401 ttagaacata taaaggctta aaagatgaaa gagtttgaag atataaacta ctcaatttgg
     2461 tgtgatttta ttgaaagaga tttcttagag aatagattcc aagagataat taatgacgaa
     2521 gttataaaag gtgcaacatc aaatcctgct atttttgaag cttcaattac aggttctgtt
     2581 gcttacaaac agcaacttga tatgttacaa gcaaatgatc aaaaaaagat atatgaagag
     2641 ttggcattta aagatataag aagagcggct gaacttttag aaccacttta taaaattgat
     2701 acaaataatg gatttatatc tattgaagtt gaccctcttt tatgtgatga tgcagctgga
     2761 actatagaag aaggacttag aatttacaaa agtttaaatg ctgataatgt aatgataaaa
     2821 attccagcaa ctgatgctgg atatattgct atgagagagt taagtagtag aggaattcat
     2881 ataaatgcaa ctttaatttt ttcaccacaa caagctagaa attgtgcaat tgcacttgat
     2941 gatggaatta aagcttcaaa caaagataca aaaggtgttg tatctgtttt tgtttcaaga
     3001 tttgatagat taatggatac aactttaggt gctaaaggtc ttgctacatc taaattaggt
     3061 attgtaaatg ctacaaaatg ttactatgaa gtaaataaat tagagaactc aaatataaga
     3121 acactttttg caagtactgg agtaaaaggg aatgagttaa gtcctacata ttatatagat
     3181 aatttgatat atccaaattc tgtaaataca gcacctcttg gaactattga agattggtta
     3241 aaagatggga aaaaagagca aagtgttatt attagtgaag atgagtgtga taaatacttt
     3301 aaaagtttag agcaaaatgg gattaaatta aaagatgttt atgaaaagct tttaagcgaa
     3361 ggtcttgatg catttaaagt atcatttaaa gagttattat caaaattaaa acattaattt
     3421 tgatttaaga tatattggat aaaatttttc caataataaa tata
//
LOCUS       DAOQAN010000055         3450 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_36821_length_3450_cov_3.508984, whole genome shotgun sequence.
ACCESSION   DAOQAN010000055 DAOQAN010000000
VERSION     DAOQAN010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 3450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3450
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36821_length_3450_cov_3.508984"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="PKO10_01505"
     CDS             complement(<1..519)
                     /locus_tag="PKO10_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW65870.1"
                     /translation="MAQKYMSYNKVLLTFTVPTDRKDKSIKNGRYTKLRTLIEIRAYI
                     AKLLNNSSDVKYFMNIELGEHYSNPHLHLQLWYKTTSSLTTNIFEKTISKFNLNKNRC
                     YITEPTHAVPIYNYVIKDYAKDLSDKQIWDLETQKKRFRKVMGSKVRFYSKSRDEFGK
                     KIYRKIYYGMGIL"
     gene            complement(791..1351)
                     /locus_tag="PKO10_01510"
     CDS             complement(791..1351)
                     /locus_tag="PKO10_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW65871.1"
                     /translation="MCKKLDPTCTECKKPFKPFHKNQLTCGKPECKKLRTNRIRKKEE
                     REFNCQVCGELSWSSNPTAVLCGKIRCRKTYQNNVNMAYGYKKKFLEKIKSGATVTGK
                     YSDKEIETLIEMKLRGHTHTDIAIALKRVFPNVDNKVRFVFSDDRFIDTIEAIRFNIE
                     AETRKANRRNVSYWNEKLNECWKNYF"
     gene            complement(1389..2036)
                     /locus_tag="PKO10_01515"
     CDS             complement(1389..2036)
                     /locus_tag="PKO10_01515"
                     /inference="COORDINATES: protein motif:HMM:NF022027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HNW65872.1"
                     /translation="MDSLIQISKNTINGVEINSVNARELYNNLGLAKGQYSRWIQNNL
                     IDQFLYNDDYIGVRQIVEGNEVVSHIVTLDTAKHLCMMAKTENAMKFRKYFIEFEKQG
                     KNLINQQSQEIQLLQGMLNTISKMDNRVTELEQTRRLENWQELALIEAKNKKVYSIAE
                     KHDLSNDKEMIRKLHSRVWKCLKKRFNVPRYNEIPAIYFNEAKTTISNLSFKDLL"
     gene            complement(2243..2584)
                     /locus_tag="PKO10_01520"
     CDS             complement(2243..2584)
                     /locus_tag="PKO10_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW65873.1"
                     /translation="MNDIIGKKTIINLPEDFICYVGDDGAISFEKFEATFSEIEGYLV
                     FSVCVDDFLWIDKNYKLTDDEEKKAFFGQIARGKNGNINYSSIHARVAAVYSALCEGK
                     IKIYELNRIED"
     gene            complement(2625..2936)
                     /locus_tag="PKO10_01525"
     CDS             complement(2625..2936)
                     /locus_tag="PKO10_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW65874.1"
                     /translation="MSILKNLKKQKNNGFILKSGIVLLLDKQEQFNKLASLFDLQEDS
                     VCIDGVYVAYMDSYPALEVCPIQDICGEIDNALEAFEHKNLKPIDFDKWYKNINAKIK
                     Y"
     gene            complement(3039..>3450)
                     /gene="ssb"
                     /locus_tag="PKO10_01530"
     CDS             complement(3039..>3450)
                     /gene="ssb"
                     /locus_tag="PKO10_01530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNW65875.1"
                     /translation="YIPSGTAVAKCSIAYTDGYGDKKTSNFMDIILWGKTAEAFNKYC
                     KIGSKVMVSGFPVFETWVDKYQQPRSRHLLRVETMKMLDSKNSDSTPPGHQYQTVRES
                     SSPRENQSTYTRKPSMDATVPEIDIDDEIPFNKG"
BASE COUNT         1121 a          579 c          389 g         1361 t
ORIGIN      
        1 taatattccc attccgtagt aaattttacg atatatcttc ttgccaaact catctctact
       61 tttagaatag aatctaacct tacttcccat aacttttcta aatctctttt tttgagtttc
      121 taaatcccat atctgtttat cactcaaatc ttttgcataa tcttttatta cataattata
      181 tattggtact gcatgagttg gttctgttat gtagcatcta tttttgttga ggttaaattt
      241 agagattgtt ttttcaaaga tatttgtagt tagtgatgag gtagttttgt accacagctg
      301 aaggtgtaga tgtggattgc tataatgctc tcctagctca atattcataa aatacttaac
      361 atcactacta ttattcaata actttgctat ataagctctt atttcgataa gagtccttag
      421 ttttgtatat cttccattct tgatgctttt atcttttcta tcagttggta ctgtaaatgt
      481 tagcaatact ttattatagc tcatatattt ttgagccata acaaaatatt catgtcttat
      541 tgaagagtat tttttacgat atgataagat tttttcagct ttagtataaa aatacttgct
      601 tttattaata gaatttgtta taatttcctc gctcaatcaa acatcttatt taaagctaga
      661 acttttcgct tggttctagc tttttttatg cctttttatt ttaaaaataa ttttttatca
      721 aaaaatacaa agtacgataa aaacggactt tggtttaaaa tatccctata taggaataaa
      781 agaaaaaaaa ttaaaaataa tttttccaac attcatttaa cttctcattc cagtaagata
      841 catttcttct attcgccttt ctagtttcag cttctatatt aaacctaata gcttcaatag
      901 tatctataaa tctatcatca gaaaaaacaa atctaacttt attatctaca tttggaaaaa
      961 ctctttttaa agcaatagca atatctgtat gagtatgccc tcttaatttc atctctataa
     1021 gagtttcaat ctctttgtct gaatatttgc cagtaacagt agctccagat ttaattttct
     1081 ctaaaaattt ctttttatat ccataagcca tatttacatt attttgatat gtttttctac
     1141 atctaatttt tccacataga acagcagtag gattactaga ccaagatagt tcgccacaaa
     1201 cttgacaatt aaactctctt tcctcttttt ttctaatcct atttgttctt agctttttac
     1261 attctggctt cccacaagta agttgatttt tatgaaaagg tttaaaaggt tttttacatt
     1321 cagtacaagt agggtctaat tttttacaca ttatgaatcc tctcaaatta atatgtaaat
     1381 atttaggatc atagcaaatc cttaaatgat aaattgctaa tagttgtttt tgcttcatta
     1441 aaataaatag ctggtatttc attatatctt ggaacattaa accttttttt aagacatttc
     1501 caaactctgc tatgtaattt tctaatcatt tctttatcgt ttgataaatc gtgcttttct
     1561 gcaatagaat aaaccttttt attttttgct tcaattaatg ccagttcttg ccagttttct
     1621 aatcttcttg tttgttcaag ctctgtaact ctattatcca ttttagaaat agtatttagc
     1681 attccttgta acagttgaat ttcttgtgat tgttgattta ttagattttt gccttgcttt
     1741 tcaaactcaa taaaatactt tctaaatttc attgcatttt cagtctttgc catcatacat
     1801 aaatgtttag cagtatcaag tgtaacaata tgtgaaacca cttcattacc ctcgacaatt
     1861 tgtcgaaccc ctatataatc atcattatat aaaaattggt caattagatt attctgaatc
     1921 catcttgaat attgaccttt agcaagtcct aagttattgt ataactctct tgcattaact
     1981 gaattaattt caacaccatt aatcgtattt tttgaaattt gaattaaaga atccatttta
     2041 tactttcaga caattaaaat taaggggaga aatttgaagt gtccagtttc actttctcgg
     2101 ctaaagccct ttatgaagca ggtagaaact ggacgaaacg acctgcttta taaaagactt
     2161 aaaaagaaag gggaaagttc ccctagctca aatttataac aacaatataa taaagaagaa
     2221 tcatatcagt acaaagctta aattaatctt caattctatt taattcataa attttaattt
     2281 ttccttcaca taatgcacta tatacagctg caactcttgc gtgaatgcta ctgtaattta
     2341 tgttgccatt tttacctcta gcaatttgac caaaaaaagc ctttttttct tcatcatctg
     2401 taagtttata gtttttatca atccataaaa aatcatcaac acaaactgaa aaaaccaaat
     2461 agccttcaat ttcactaaaa gtagcttcaa atttttcaaa actaattgca ccatcatctc
     2521 caacataaca aataaaatct tctggaagat ttataatagt ttttttacca attatatcat
     2581 tcataatttt tacccttttt atatcaaata tctctaaata ttttttaata tttaattttt
     2641 gcattaatgt ttttatacca cttatcaaaa tcaataggtt ttagattttt atgctcaaaa
     2701 gcttctaaag cattatcaat ttcaccacat atatcttgta taggacaaac ctctaaagca
     2761 gggtaagaat ccatatacgc aacataaact ccatctatac aaacactatc ttcttgtaaa
     2821 tcaaaaagag aagcaagttt attaaactgc tcttgcttgt ctaaaagcaa aacaatccca
     2881 gattttaaaa taaatccatt atttttttgt ttttttaaat ttttcaaaat actcatataa
     2941 aatcaccttt tttcgtaaaa tttaattttg gaaaaaatcc aaaaaaattg gtgggaaccc
     3001 ctactatcgt tttttagcta tttggataaa ggtatagcct aacctttatt aaaaggtatt
     3061 tcatcatcaa tatctatttc tggaactgtt gcatccattg atggttttct agtataagtg
     3121 ctttgatttt ctcttggact agaactctct cttactgttt ggtattgatg tccaggaggt
     3181 gtagagtcgc tattttttga atctagcatt ttcattgttt cgactcttaa taaatgccta
     3241 cttctgggct gttggtattt atcaacccaa gtttcaaaaa caggaaaccc actaaccata
     3301 acttttgaac caattttaca atatttatta aatgcttcag cagtttttcc ccataaaata
     3361 atgtccataa aatttgaagt ttttttatct ccatagccat ctgtatatgc gatagaacat
     3421 ttagctactg ctgtaccact aggaatatat
//
LOCUS       DAOQAN010000056         3365 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_38164_length_3365_cov_2.874622, whole genome shotgun sequence.
ACCESSION   DAOQAN010000056 DAOQAN010000000
VERSION     DAOQAN010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 3365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3365
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38164_length_3365_cov_2.874622"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /gene="truB"
                     /locus_tag="PKO10_01535"
     CDS             complement(<1..297)
                     /gene="truB"
                     /locus_tag="PKO10_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014475283.1"
                     /note="catalyzes isomerization of specific uridines in RNA
                     to pseudouridine; responsible for residues in T loops of
                     many tRNAs; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HNW65876.1"
                     /translation="MQKRVYEKGEINKLFVVNKPMFISSNFYLNRFKRAYKNKKAGFS
                     GTLDPFAKGCLIVAFGQYAKLFKYLEKTPKVYKAVIWLGVTSESLDIERIQDIVI"
     gene            complement(307..2361)
                     /locus_tag="PKO10_01540"
     CDS             complement(307..2361)
                     /locus_tag="PKO10_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNW65877.1"
                     /translation="MSENLLISLNNSQKSAAQHIDGPLLILAGAGSGKTKTITTRLAY
                     LISIGIDPKTILTLTFTNKAANEMRDRAFSLLDTNSLNTPPLLCTFHKFGLLFLKFYI
                     SELNRKNNFIIIDSDDKRRVLKSINKEIPTALLVSEVSKYKNSIMTPIEVKSQAQGKL
                     YSEIADIYEKYEEHLLKNNLVDFDDLLLLPYKILKNNQKLAESTSQKYQYIMVDEYQD
                     TNELQYRLLRLLCSNHDNLCVVGDDDQSIYGWRGATIKNILNFNEHFKRATIVKLEEN
                     YRSTDTILHHANALIEHNRDRLGKKLVGTKDKGSSIKVYESHDENEETRKIVDDIKKL
                     LQSDEKAKDIAILFRVNALSRSLEEGFNKAGLNYKLVGGMKFYERTEIKDLIAYFRIL
                     TNLSDNFSIKRVINKPKRGIGATTIDKLEEKANELNSSIFDMIQNLNADELSAIVGKK
                     NARTIKVFEASILDLRELMNQSKMRLLDSFEETFDYRASYDNLPDGFERQANIDEFYG
                     YIRDFFLQNPHLDLKDFLNEIALENQNDEYSNDAISMMSIHSSKGLEFKHLFIIGLEE
                     GFFPIIGDGTDLEEERRLGYVAFTRAMDNLTLSFVHSRFYKGKRTQLNKSRFLVESGL
                     VKGSLVIEKTSGFKKGDMVSHKIFGQGRVEKAVNAGKDYKLTINFGGQKRDILSSFVE
                     KN"
     gene            2476..>3365
                     /locus_tag="PKO10_01545"
     CDS             2476..>3365
                     /locus_tag="PKO10_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNW65878.1"
                     /translation="MLNDFAKLETFLTVVREKSFSKASAKLGISQPAVTQQMKFIEDY
                     LDVQIVDRKKNGIKLTKEGQILHGIALKIEKCVNSAEKELLKIMNKNTTFVFGASFII
                     GNYILPRFLNNLKENIHNDVSINVSVSHEAIEDLLDKKIDIALVENYIANDEIIYREW
                     MEDEIVIFSNQKLPARAKPEDLLSYKWICRNPESNTGSIFKECLEKANYPDCDTFEVT
                     SEVTSATTIVQTVLHSDKNATPAVSIVSRNAIESLLKSGALYESRIGNQKMLRKLYIA
                     YRKDRKHDAFIENVVDYLLK"
BASE COUNT         1162 a          517 c          392 g         1294 t
ORIGIN      
        1 aattactata tcttgaattc tctcaatgtc taaagattca gaagtaactc caagccaaat
       61 aacagcttta taaacttttg gagttttttc taaatattta aaaagctttg cgtattgtcc
      121 aaatgcaacg atcaaacaac cttttgcaaa aggatcaaga gttccactaa atccagcttt
      181 tttattttta taagctcttt taaatctatt taaataaaaa tttgaactaa taaacattgg
      241 tttatttaca acaaaaagct tatttatttc acctttttca taaactcttt tttgcaaatt
      301 ttacctttag tttttctcta caaaagatga caaaatatct cttttttgtc caccaaaatt
      361 tatagtaagc ttataatctt ttccagcatt tacagctttt tcaactcttc cttgtccaaa
      421 aattttatgg cttaccatat cacctttttt aaatccagaa gttttttcta taactaaact
      481 tccttttact aaaccactct caaccaaaaa tctactttta tttaattgag ttcttttacc
      541 tttataaaat cttgaatgta caaaagatag agttaaatta tccatagctc ttgtaaatgc
      601 aacatatcct aatcgtctct cttcttccaa atctgtgcca tctccaataa ttggaaaaaa
      661 tccctcttca aggccaataa taaacagatg tttaaactct aaaccttttg aagagtgaat
      721 actcatcata gaaatagcat cgttgctata ttcatcattt tgattttcta atgctatttc
      781 atttaaaaaa tcttttaaat ctaagtgtgg attttgtaag aaaaaatctc ttatatatcc
      841 ataaaattca tctatatttg cttgtctttc aaaaccatct ggaagattat cataagaagc
      901 tctataatca aaagtctctt caaaactatc taaaagtctc atttttgact gattcataag
      961 ctctcttaaa tctaaaatag atgcttcaaa aacttttata gttcttgcat ttttcttacc
     1021 aacaattgca cttaattcat cagcatttaa attttgaatc atatcaaaaa tagaactatt
     1081 taactcattt gctttctctt caagtttatc aatagtagtt gcccctattc ctctttttgg
     1141 tttatttata actcttttta tagaaaaatt atcacttaag tttgttaaaa ttctaaaata
     1201 tgctattaaa tcttttatct ctgttctttc ataaaatttc attccaccaa caagtttata
     1261 attaagccct gctttattaa atccctcttc taaagaacga ctaagagcat ttactctaaa
     1321 taaaattgca atatctttag ctttttcatc tgattgtaac aattttttta tatcatctac
     1381 tatttttctt gtctcttcat tttcatcgtg tgattcataa acttttatag aactaccttt
     1441 atcttttgta cctacaagtt ttttacctaa tctatctcta ttgtgctcaa ttaaagcatt
     1501 tgcatggtgt aaaatagtat cagttgatct ataattttct tcaagtttaa caatagttgc
     1561 tcttttaaaa tgttcattaa aattcaaaat atttttaatt gttgcccctc gccatccata
     1621 aatactttga tcatcatctc caacaacaca aagattatca tggtttgagc aaagaagcct
     1681 taaaagtcta tattgaagct catttgtatc ttgatactca tctaccataa tatattgata
     1741 cttttgactt gtagattctg ctaatttttg attattcttt aaaattttat aaggcaaaag
     1801 aagtaaatca tcaaaatcta caagattgtt ttttaacaaa tgctcttcat atttttcgta
     1861 aatatctgca atttcactat aaagtttccc ttgagcttgg gattttactt caattggagt
     1921 cataatagag tttttatatt ttgaaacttc acttactaaa agtgctgttg gtatctcttt
     1981 atttattgat tttaaaactc ttcttttgtc atcactatca attattataa aattattctt
     2041 tctatttaat tcacttatat aaaattttaa aaaaagaagt ccaaatttgt gaaatgtaca
     2101 taaaagtgga ggtgtattta aactatttgt gtctaaaaga gaaaaagctc tgtctcgcat
     2161 ctcatttgca gctttatttg taaaagtaag cgttaaaata gtttttggat ctattccaat
     2221 cgatattaaa taggccagtc tagttgtaat tgtttttgtt tttccacttc cagcacctgc
     2281 taaaatcaac aaaggaccgt ctatatgttg tgcggcactt ttttgtgaat tgtttagtga
     2341 tattaataaa ttttcagaca ttttttctcc aataatttag attataccat aaaatttata
     2401 aaaatattat atctcttaat tattctatat ataaataaat gttatcattt tgttaattta
     2461 tttttggaga atttaatgct caatgatttt gctaaattag agactttttt aactgttgta
     2521 agagagaagt ctttttcaaa agcatccgct aaactaggta tttctcaacc agctgttact
     2581 caacaaatga agtttataga ggattatcta gatgttcaaa ttgttgatag aaaaaaaaat
     2641 ggtattaaac taacaaaaga gggtcaaatt cttcatggaa ttgctttaaa aattgaaaag
     2701 tgcgttaata gtgctgaaaa agaactttta aaaattatga ataaaaatac aacttttgtt
     2761 tttggtgcta gttttataat aggaaattat attcttccta ggtttttgaa taatcttaaa
     2821 gagaatattc ataatgatgt atcaataaat gtatctgttt cacatgaagc tatagaagat
     2881 ttactagata aaaaaataga tattgcactt gttgaaaact atattgcaaa cgatgagatt
     2941 atttatagag agtggatgga agatgaaata gtaatattct caaaccaaaa gcttccagca
     3001 cgtgcaaaac ctgaagattt attatcttac aaatggattt gtagaaatcc tgagtcaaat
     3061 acagggtcaa tttttaaaga gtgtcttgaa aaagcaaact atcctgactg cgatacattt
     3121 gaagttacaa gtgaagttac aagcgctaca actattgttc aaactgtttt acactctgat
     3181 aaaaatgcaa ctcctgctgt atctattgtt tcaagaaatg ctattgaatc actacttaag
     3241 tcaggtgctt tatatgaatc aagaattggc aatcaaaaaa tgctaagaaa actatatatt
     3301 gcatatagaa aagatagaaa acatgacgct tttatagaaa atgttgtgga ttatttatta
     3361 aaaat
//
LOCUS       DAOQAN010000057         3336 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_38651_length_3336_cov_2.521183, whole genome shotgun sequence.
ACCESSION   DAOQAN010000057 DAOQAN010000000
VERSION     DAOQAN010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38651_length_3336_cov_2.521183"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="PKO10_01550"
     CDS             <1..220
                     /locus_tag="PKO10_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014474761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HNW65879.1"
                     /translation="DIKRVKDEENLHTVALLLTDIMKEGSEVLVASDDASIFEKAFNC
                     KLEDGKVWLDGCLSRKKQIIPFLEPAFA"
     gene            270..1475
                     /locus_tag="PKO10_01555"
     CDS             270..1475
                     /locus_tag="PKO10_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014469049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HNW65880.1"
                     /translation="MKKYLIEFLKDIGIEPNFIAMSISILLIIAITSIVIHLVLHKII
                     LKAIKKLDLKKRNIITTSLLEENLFQRLALVFQGLVVYWQATIWVEKGAIYETLLTIS
                     LVWITIFGLLAIYSLIDKTFKTLHAATEVQFFAMRTIIQSIKLIFGIIALIYIIAILA
                     DKSPVAILSGLGAMSAVLMLVFKDTILGFTAGLQLSTNKMVQIGDWIEMPKYGADGDV
                     IDIGLNVVKVRNFDKTITTIPTYALVSDSFKNYRGMRESGGRRIKRAIKIDIHSIKFL
                     SEEDIKRLEKANLLAPYLQEKKNEIKNYNESNKFDLSVAMNGRRLTNIGTLRAYILTY
                     LKNHPNINKDMTIMVRQLAPDEYGIPLEIYCFTSTTVWADYENIQSDIFDHIYSILQE
                     FDIKPYQYG"
     gene            1523..2308
                     /locus_tag="PKO10_01560"
     CDS             1523..2308
                     /locus_tag="PKO10_01560"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNW65881.1"
                     /translation="MKSYIFKNPKLDFIELRYVEDIKDCVKMHLHEELTITAIKKGSL
                     TLIFNDTSITVKANEIAIINSQIPHSATTNEKSKDGYVLYLKKDYLKNLDFNFSSAFE
                     LIKNRNIYKGFIKLCDCLLDIKVSLIEKEENLYLFCFAFFSFEQTEQNYKEESTLAMN
                     IKKYLDESYLEEFILDELALKFDLTVVHLIRVFKKEFGLPIHSYILNKKVHLAKELLT
                     SNISISQIEQNSGFFDQSHLNRSFKRIFQITPKEYQKNIFSKC"
     gene            2488..>3336
                     /locus_tag="PKO10_01565"
     CDS             2488..>3336
                     /locus_tag="PKO10_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014474758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HNW65882.1"
                     /translation="MSFKKYQQYPIVQNFKRKWADKTITHAPIYCSVDLRDGNQALIN
                     PLTVEQKLEYFKALVKMGFKQIEVSYPSASDTDFNFTRKLIEEDLVPDDVAIQILIPA
                     KKEWIKKSVEAMKDVKNGIFHLYNPTNEFQRRVVFQKSDDEIIQMAIEAMQYLVELTK
                     DFKGKVTYQYSPESFSQTDLEFAVKICNEVINVVKPTVENKMIINLPNTLEACMPNVY
                     ADRIEWMCKNLHNREALIISVHPHNDRGTSVASAELAVLAGADKVEGTLFGNGERAGN
                     LDIINFA"
BASE COUNT         1309 a          375 c          540 g         1112 t
ORIGIN      
        1 tgatataaaa agagtaaaag atgaagaaaa tcttcacacg gttgcactac ttttaactga
       61 tattatgaaa gaaggtagtg aggttttagt agctagtgat gatgcttcaa tatttgaaaa
      121 agcatttaac tgtaaacttg aagatggaaa agtttggtta gatggatgtt taagtagaaa
      181 aaaacagatc attcctttct tagagcctgc gtttgcttaa gatataaaag ccttttggct
      241 tttatatctt aattccttag gagaaaaaaa tgaaaaaata tttaatagaa tttttaaaag
      301 atataggaat agagccaaat tttattgcaa tgagtatctc tattttactt attattgcaa
      361 ttacttctat agtaatccac ctagttttac ataaaattat cttaaaagct atcaaaaaac
      421 ttgatttgaa aaagagaaat attattacta catctttgct tgaagagaat ttatttcaaa
      481 gattagctct tgtttttcaa ggattggttg tatattggca agcaactatt tgggttgaaa
      541 aaggtgcgat ttatgagaca cttttaacca tctctttggt ttggataaca atttttggac
      601 ttttggcaat atactctttg atagataaaa catttaaaac tcttcatgct gcaacagaag
      661 ttcaattttt tgctatgaga acgattattc aaagtataaa acttattttt ggaataattg
      721 cacttattta tataatagct attttggctg ataaatcacc agttgccata cttagtggtt
      781 tgggagctat gtcagcagtt cttatgttgg ttttcaaaga tacaatcttg ggatttacag
      841 caggtcttca actaagtaca aataagatgg tacaaattgg agattggata gagatgccaa
      901 aatatggggc agatggagat gttattgata ttggattaaa tgttgtaaag gttagaaact
      961 ttgataaaac aattacaact attccaactt atgctttagt ttctgatagt tttaaaaatt
     1021 atagaggtat gcgagaatca ggtggaagaa gaataaaaag agctataaaa attgatatac
     1081 atagtattaa attcttaagt gaagaagata taaaaaggct tgaaaaagcg aatcttttag
     1141 ctccttattt gcaagagaaa aaaaatgaga tcaaaaatta taatgaatca aataaatttg
     1201 atttaagtgt cgcaatgaat ggacgaagac ttacaaatat tggaacatta agagcgtata
     1261 ttttgacata tttaaaaaat catccaaata taaataaaga tatgacaata atggttagac
     1321 aattagcacc tgatgaatat ggaattccac ttgagatata ttgctttact tctacaacag
     1381 tttgggctga ttatgaaaat atacaatcag atatttttga tcatatctat tctattttgc
     1441 aagaatttga tataaaacca tatcaatatg gataaaaata tataattgaa aatattttca
     1501 attatatatt aaagtttaaa aaatgaaaag ttacatattt aaaaacccaa aactagactt
     1561 tatagagtta cgatatgtag aagatataaa agattgtgta aagatgcatt tacacgaaga
     1621 acttacaatc actgcaataa agaaaggttc attaactctt atttttaatg atacttcaat
     1681 aacagtaaaa gcaaatgaaa ttgctattat aaatagtcaa attccccata gtgcaacaac
     1741 aaatgaaaaa tcaaaagatg gatatgtttt atacttgaaa aaagattatt taaagaattt
     1801 agattttaat ttctcatcag cttttgagtt aatcaaaaat agaaacatct ataaaggttt
     1861 tataaaatta tgtgattgtt tacttgatat taaagtctct ttgatagaaa aagaggaaaa
     1921 tctttatcta ttttgttttg catttttctc ttttgaacaa acagaacaaa actataaaga
     1981 agaatcgact ttagcaatga atataaaaaa atatttagat gaaagttatc ttgaagagtt
     2041 tattttagat gagttagctt tgaagtttga tttaacagtt gttcatctaa tacgagtatt
     2101 taaaaaagag tttggacttc caattcattc gtatatttta aataaaaaag ttcatcttgc
     2161 aaaagagtta ttgacttcaa atatctctat ttctcaaata gagcaaaata gtggtttttt
     2221 tgaccaaagc cacttgaata gaagttttaa aagaatcttt caaattacac caaaagagta
     2281 tcaaaaaaat attttttcaa aatgttaatt ttgtacaaga ttttattttt tgtaaaagtt
     2341 ataatttgca aaatttaaaa aggagtttat catggatgta aatattataa aacaaattat
     2401 ctctttttct ctgctttgcc tcaaaacttt ctaatactta aaatataaaa aacaaatcaa
     2461 aaaataaaaa tcataacgga gtaaaccatg agtttcaaaa aatatcaaca atatccaatt
     2521 gtgcaaaatt ttaaaagaaa gtgggcagat aaaactataa cacacgcacc aatttattgt
     2581 agtgtagatt taagagatgg aaatcaagct ttgataaatc ctttaacagt tgagcaaaaa
     2641 ttggagtatt ttaaagcttt ggtaaaaatg ggctttaaac aaatagaggt tagttatcca
     2701 agtgctagtg atacagattt taactttact agaaaattaa tcgaagagga tttagtgcca
     2761 gatgatgtgg ctattcagat tttaattcct gcaaaaaaag agtggattaa aaaaagtgta
     2821 gaagctatga aagatgtaaa aaatgggatt tttcatttat acaatccaac aaatgagttt
     2881 caaagaagag ttgtttttca aaaaagtgat gatgagataa tacaaatggc cattgaagct
     2941 atgcaatatt tagttgaact aacaaaggat tttaaaggaa aagtaactta ccaatattca
     3001 ccagaaagct tttctcaaac tgatttagag tttgctgtaa agatttgtaa tgaagttata
     3061 aatgtagtaa aacctacagt tgaaaataag atgataataa acttaccaaa tactcttgaa
     3121 gcttgcatgc caaatgttta tgcagataga atagagtgga tgtgtaagaa tttacacaat
     3181 cgtgaagctt tgattattag tgttcatcca cacaatgata ggggaacttc tgtggcaagt
     3241 gctgagttag ccgtacttgc aggggcagat aaagttgaag gaacattgtt tggaaatggt
     3301 gaaagagcag ggaacttaga cataataaat tttgct
//
LOCUS       DAOQAN010000058         3304 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_39202_length_3304_cov_2.598954, whole genome shotgun sequence.
ACCESSION   DAOQAN010000058 DAOQAN010000000
VERSION     DAOQAN010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3304
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39202_length_3304_cov_2.598954"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="PKO10_01570"
     CDS             complement(<1..480)
                     /locus_tag="PKO10_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012148124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HNW65883.1"
                     /translation="MRLNFGILKRPNSPIPGFGLTMGYTVFYLSIIVLIPLIALFTEA
                     FSMGFDAFISASTDARVVASYKLTFVTSLIAALINTIFGVIVAWCLVRYTFFGKRVFD
                     AIVDLPFALPTAVSGIALTTLYSSSGWFGQYLEPLGIKIVFTPIGITIALIFIGLPFV
                     "
     gene            complement(607..1830)
                     /locus_tag="PKO10_01575"
     CDS             complement(607..1830)
                     /locus_tag="PKO10_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014475521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNW65884.1"
                     /translation="MVKKESKNNEILLPNRKAFLKDVENGFFNKLVIFDISGFGNINH
                     YYGYDFGEKILKIISVRLQERFENSQMYYLGGDIFVATASEDISKERFIQTIKATTWH
                     FGYSPIELDGHKVYIPLRAGVAINYPELLFCAEFALKRTRVLKHNLVIFDSEQHQLCH
                     PNSLTIEQDLYWEAQIIQAIKKDRFEIFAQSISNQNDKKYEILVRMKDTKGEIVSPYF
                     FIDRAKKINLYGEITKKVVQKSFEFFEGKNIEFSINLSISDILEKDICDFLIQKICEH
                     DVGYFLTIEITESEGVENIEELVSFIKIVKNLGVKIAIDDFGTGYSNFSYLVKLQADF
                     IKLDGSIIQDINKSLSAKAVVEAIVFFAKKVGIKTVAEFISSKEIFKTCKELEIDYFQ
                     GYLLDEPKNIKELRL"
     gene            complement(1862..3010)
                     /gene="moeB"
                     /locus_tag="PKO10_01580"
     CDS             complement(1862..3010)
                     /gene="moeB"
                     /locus_tag="PKO10_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012148118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HNW65885.1"
                     /translation="MSEISKQTRLSQEEINRYSRHLILPEVGLKGQLKLKNSKVLVVG
                     TGALGSPVLLYLSAAGIGTIGIIDFDTVDESNLQRQIIHSTNEIGNPKVNSAKSRINS
                     INPNIDVIAYNDKLTSKNALEIIKNFDVVVDGTDNFQTRYLINDACVFLNKPFVYGAI
                     FRFEGQSTVFNADKNSPCYRCIFPEPPKAGLVPSCSEAGVLGVLPGIIGTIQASETIK
                     TVLEIGETLKGRLLTLNALKMDFKDYKIKKDENCKVCGENATITELIDYEEFCGLKDS
                     LQEIEFKELSGENLKELIHNKEQILLIDVRISADFQARNIYNSKNIPFEDLLNRVSEI
                     DETKKVVLICTIGLKSKEAISKLKKAGYKGELYSLKGGITSWINDNQD"
     gene            complement(3083..>3304)
                     /locus_tag="PKO10_01585"
     CDS             complement(3083..>3304)
                     /locus_tag="PKO10_01585"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNW65886.1"
                     /translation="NGRFEIALQGESIKDIVGNLLKEHKDLESHIYTNDGKLKNYVNI
                     YLNEKNINRLDNFDTKVYEDDKIILFQAS"
BASE COUNT         1138 a          554 c          329 g         1283 t
ORIGIN      
        1 aacaaaagga agcccaataa atattaaagc aattgtaatc ccaataggtg taaatactat
       61 tttaattcca agtggctcta aatattgtcc aaaccaacca gaacttgaat ataaagttgt
      121 aagtgcaata cctgaaacag ctgttggaag agcaaaaggt aaatcaacaa ttgcatcaaa
      181 gactcttttc ccaaaaaatg tatatcgcac caaacaccaa gcaacaatta ctccaaaaat
      241 tgtattaata agagcagcta ttagtgaagt tacaaaagtc aatttataac tagctaccac
      301 tcttgcatct gttgaggcac ttataaaagc atcaaatccc atactaaaag cttcagtaaa
      361 aagtgcaatt agtggaatta atacaataat acttaaataa aatacggtat atcccatagt
      421 caaaccaaaa ccaggaattg gtgaatttgg tctttttaat attccaaaat ttaatctcat
      481 ttaaaccctt tttatataac atacactaga ttagtgaatg ttaaaattaa acaaaaaaat
      541 agatgattaa attaatcatc tataaaattg gtgaattata actatttctt ttttatttgt
      601 caagaattat agtcttaact ctttaatatt tttaggctca tctaataaat atccctgaaa
      661 ataatcaatc tctagctctt tacaagtttt aaaaatctct tttgatgata taaactcagc
      721 aactgttttt ataccaactt ttttcgcaaa aaatactatt gcttcaacaa ctgctttagc
      781 agaaagagat ttatttatat cttgaataat agaaccatct aattttataa aatcggcttg
      841 taatttaacc aaatatgaaa aatttgagta accagttcca aaatcatcaa ttgcaatttt
      901 tacacctaaa tttttaacta tttttataaa agatactagt tcttctatat tttcaacacc
      961 ttcgctctct gttatttcta tagttaaaaa gtaaccaaca tcatgttcac atattttttg
     1021 aattaaaaaa tcacaaatat ctttttccaa aatatcacta atcgataaat ttatactaaa
     1081 ctctatattt ttaccctcaa aaaattcaaa tgatttttga actacttttt ttgttatttc
     1141 tccatatagg tttatttttt ttgctctatc tataaaaaaa taaggagata ctatctctcc
     1201 tttggtatct ttcattctta ctaatatttc atatttttta tcattttgat tactaattga
     1261 ttgtgcaaat atttcaaatc tatctttttt tatagcttga ataatttgag cttcccaata
     1321 taaatcttgc tcaattgtta aagagtttgg atgacataat tgatgttgtt cactatcaaa
     1381 aattacgaga ttgtgtttta agactcttgt tcgttttagc gcaaattctg cacaaaatag
     1441 aagttctgga tagtttatag caactccagc tcttaaggga atatatactt tatgcccatc
     1501 tagttcaatt ggtgagtatc caaaatgcca agtagttgct tttatagttt gaataaatct
     1561 ctcttttgaa atatcttcac ttgcagttgc aacaaaaata tctccaccta aatagtacat
     1621 ctgactattt tcaaatctct cctgaagtct tacagagatt atttttaata ttttttctcc
     1681 aaaatcatat ccatagtagt gatttatatt tccaaaacca cttatatcaa atattacaag
     1741 tttattaaaa aagccatttt ctacatcttt taaaaaagct tttctattgg gtagtaaaat
     1801 ctcattattt ttactctctt tctttaccac atttagctcc ttttaaaatg aaaagaggtt
     1861 tctaatcttg attatcattt atccaagatg taatcccacc ttttaaacta taaagttctc
     1921 ctttataacc agcttttttt aattttgaaa tagcctcttt acttttaagt cctattgtac
     1981 aaatcaatac aacttttttt gtttcatcta tctcagatac tctatttaac aaatcttcaa
     2041 atggtatatt ttttgaatta taaatatttc tagcttgaaa atcggcgcta attctaacat
     2101 caattaacaa aatttgctct ttgttatgaa ttaactcttt taaattttcg ccacttaact
     2161 ctttaaactc aatctcttgt aaactatctt ttaatccaca aaactcttca taatctataa
     2221 gttctgttat agttgcattc tctccacaaa ctttacaatt ctcatctttt tttattttat
     2281 aatctttaaa atccattttt aaagcattta aagtaagtaa tcttcccttt aatgtttccc
     2341 ctatttctaa aactgttttt atagtttcac ttgcttgaat agtcccaata attccaggta
     2401 aaactcctaa aactccagct tctgagcaag atggaacaag accagctttt ggaggttctg
     2461 gaaaaataca tctatagcaa ggactatttt tatctgcatt aaaaactgtt gattgtcctt
     2521 caaatctaaa aattgctcca taaacaaaag gtttatttaa aaatacacaa gcatcattta
     2581 tcaaatatct tgtttggaaa ttatctgttc catctactac gacatcaaag ttttttataa
     2641 tctctaaagc atttttgctt gttaatttat cattataagc tattacatct atatttggat
     2701 ttattgaatt tattctactt ttagctgagt ttacttttgg atttcctatt tcatttgttg
     2761 agtgaataat ctgtctttgc aaatttgatt catcaactgt atcaaaatca attattccaa
     2821 tagttccaat accagctgca ctaagatata aaagaacagg agaacccaaa gctcctgttc
     2881 caacaactaa aacttttgaa ttttttaatt ttagttgtcc ttttaatcca acttctggaa
     2941 gtattaaatg cctactatat ctatttatct cttcttgcga aagtctagtt tgttttgaaa
     3001 tctctgacat aattatcctt taaaattcaa ctgcaaggat aacacaagag atatttatat
     3061 tatcttttat ttgatataca aactatgatg cttgaaataa aattatcttg tcatcttcat
     3121 aaacttttgt atcaaaatta tctaatctat ttatattctt ttcatttaaa tatatattta
     3181 cataattttt taactttcca tcatttgtat atatatgact ttctaaatct ttatgctctt
     3241 ttagtaaatt tcctacaata tctttgatac tttcaccttg taaagcaatt tcaaatctac
     3301 catt
//
LOCUS       DAOQAN010000059         3285 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_39539_length_3285_cov_3.176161, whole genome shotgun sequence.
ACCESSION   DAOQAN010000059 DAOQAN010000000
VERSION     DAOQAN010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39539_length_3285_cov_3.176161"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(45..722)
                     /gene="tenA"
                     /locus_tag="PKO10_01590"
     CDS             complement(45..722)
                     /gene="tenA"
                     /locus_tag="PKO10_01590"
                     /inference="COORDINATES: protein motif:HMM:TIGR04306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050334 - thiaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HNW65887.1"
                     /translation="MSFSRSLKQKAIKVWEDGYNHPFVQELGAGTLSKDKFKFYLLQD
                     YLYLLEYAKVFAMALTKADDEKMLSNLSAITKATLVDEMKLHHLYMKEFGISDEEVQN
                     VRASLFNRTYTANMLATALKGDLAQTLATVFPCAWTYCDYGKRLKEQYKDTLENNFYK
                     SWIETYSGAEFEESFEWFYDALDELVANKSEAEKKVVEDIFISSVEFEYMFWDMAYNK
                     QMSYVKS"
     gene            complement(739..1560)
                     /gene="thiM"
                     /locus_tag="PKO10_01595"
     CDS             complement(739..1560)
                     /gene="thiM"
                     /locus_tag="PKO10_01595"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: protein motif:HMM:NF006830.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HNW65888.1"
                     /translation="MIDNLIDILNNTKKQNPLIHHITNYVTVNDCANVTLAVGASPAM
                     ADDEAEVEEFVNIASALLINIGTLNKDIKNSILKAVKKANELNVPVILDPVGVGASKF
                     RKDFINELLETYKISSIRGNISEIKSILNLSSNTKGADASKEDLESIDNTLNIAKELA
                     NKLSLVVAITGETDVISDGKRVVTIKNGNYLLPYITGTGCMCSSLVASYCGASNDKIF
                     EATVLGVITMGIAGEIAYENSNNKGLGTFHKELFDAISNFNKDILIKKANFQEIK"
     gene            1662..3173
                     /locus_tag="PKO10_01600"
     CDS             1662..3173
                     /locus_tag="PKO10_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008336659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HNW65889.1"
                     /translation="MKFLITSLIFCTLFVNMQASTLNLSMSSSPSRLNPILANDSASG
                     EISDWLFNGLFKYDKDGNITTEIASSYTFETPTKLIVKLKDNVLWHDKEKLSSKDVVF
                     TYEQIISPTVFNSIKSNFSEVASVKALDDLTLEIIYKKPYFKALEIWMVGLLPYHILK
                     DEKNLMTSSFNKNPIGTGSYKLKEFKTGQDIELIANDDFFEGRPKIDRILYKFLPDPN
                     TSFLYLKQSKLDIGSLTPMQVSRQLDSSFKNNFEILNRPSFGFTYLGFNLENPKFKDI
                     KVRQALSFAINRQELVDILFFGYGEVCNGPFMPNSFAYNDDVKPITQDLLKAKALLKE
                     AGYDEKNPLVFEVVTNTGNDIRVNTAQILQYQLSKIGVQMKIRVMEWQAFLNTVVHPR
                     KFETVLLGWSLALMPDAYPLWHSKSAKLGGFNLVNYKNEKVDELIEKGSGTINKDELG
                     AIYKEIFKIVANDLPYLFLYIPDGITAINKKIENIDPAFIGIMHNQKDWEIKE"
     gene            3185..>3285
                     /locus_tag="PKO10_01605"
     CDS             3185..>3285
                     /locus_tag="PKO10_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014472960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNW65890.1"
                     /translation="MNKKDKIILFDLDGTLIDSTDAITDTFLHAFSK"
BASE COUNT         1184 a          504 c          423 g         1174 t
ORIGIN      
        1 caaaggaagc gattttatcg cttccttttt ttatgccaaa tattttagct ttttacataa
       61 ctcatctgct tattataagc catatcccaa aacatatact caaactcaac acttgaaata
      121 aaaatatctt caacaacttt tttctcagct tcacttttat ttgctacaag ctcatcaagt
      181 gcatcataaa accactcaaa cgactcttca aactctgctc ctgagtaagt ctctatccaa
      241 gatttataga agttattttc aagagtatct ttatattgct cttttaatct tttaccataa
      301 tcacaatatg tccaagcgca aggaaataca gttgctaaag tttgcgctaa atcacctttt
      361 agagcagttg caagcatatt tgcagtataa gttctattaa acaaactagc tcttacattt
      421 tgaacctctt catcactaat accaaactct ttcatgtata aatggtgaag tttcatctca
      481 tcaactaaag ttgcctttgt aatagcactt aggttactca acatcttctc atcatctgct
      541 tttgtcaaag ccattgcgaa aacttttgca tactctaata aatatagata atcttgtagt
      601 aaataaaatt taaacttatc ttttgataaa gtaccagctc ctaactcttg tacaaatgga
      661 tgattataac catcttccca aacttttatt gctttttgtt ttaaacttct agaaaatgac
      721 atttttattc ctttataatt attttatctc ttgaaaattt gcttttttta ttaatatatc
      781 tttattaaaa ttacttattg catcaaataa ctctttatga aaagttccaa gtcctttatt
      841 attactattt tcataagcta tttcaccagc aattcccatt gtaattactc ctaaaactgt
      901 agcttcaaat attttatcat ttgaggctcc acaataacta gcaaccaaag agctacacat
      961 acaacctgtt cctgtaatat atggcaaaag atagttacca tttttaatag taacaactct
     1021 tttaccatca gatataacat cagtttctcc agtaattgcc acaactaaac ttaacttatt
     1081 tgctaactct tttgctatat ttaatgtgtt atcaattgat tccaaatcct ctttagaagc
     1141 atcagcacct tttgtgtttg agcttaaatt taatatagac tttatttcag aaatatttcc
     1201 tctaatagaa gatattttgt atgtttcaag taactcattt ataaaatctt ttctaaactt
     1261 tgacgcacct acaccaactg gatctaaaat aactggaaca tttaattcat ttgctttttt
     1321 tacagctttt agtatagagt tttttatatc tttatttaat gttccaatat ttattaaaag
     1381 agctgaagca atattcacaa actcttcaac ttctgcttca tcatctgcca tagcaggact
     1441 tgcaccaact gctaaagtta catttgcaca atcatttact gtaacatagt ttgtaatgtg
     1501 atgaattaat gggttctgtt tttttgtatt atttaaaata tcgattaaat tatctatcaa
     1561 agtaatgcct taaaatatat taataaagta gctattctat cacaaagaaa aatgaaaagc
     1621 taatttataa tactttttat aaaaaaacta tacaatacgc aatgaaattt ttaataactt
     1681 ctttaatatt ttgtactctt tttgttaata tgcaagcaag tacactaaac ttatctatga
     1741 gttcaagtcc aagtagatta aatcctattt tagcaaatga tagtgcaagt ggtgaaatat
     1801 ctgattggct ttttaatggt cttttcaaat atgataaaga tggaaatata acaactgaaa
     1861 tagcatcttc atacactttt gaaacaccaa caaaacttat agttaaacta aaagataatg
     1921 ttttatggca tgacaaagaa aaactatctt caaaagatgt agtttttact tatgaacaaa
     1981 taataagccc aactgttttt aactccataa aatcaaattt tagtgaagtt gctagtgtaa
     2041 aagctcttga tgatttaact cttgaaataa tctataaaaa accatatttt aaagccttag
     2101 aaatttggat ggttggtctt ttaccatacc atattttaaa agatgaaaaa aatcttatga
     2161 caagtagctt taataaaaat ccaataggta caggctctta taaattaaaa gagtttaaaa
     2221 ctggacaaga tatagaactt attgcaaatg atgatttttt tgaaggtcgt cctaaaattg
     2281 ataggatttt atataaattt ttaccagacc caaatacatc ttttttgtat cttaaacaga
     2341 gcaaacttga tattggaagc cttactccta tgcaagtatc aagacagtta gatagtagct
     2401 ttaaaaataa ttttgaaatt ttaaatagac ctagttttgg atttacatat ttaggattta
     2461 atttagaaaa tcccaaattt aaagatatta aagtaagaca agctttatca tttgctataa
     2521 atcgacaaga gctagttgat attttgtttt ttggatatgg agaggtttgc aatggaccat
     2581 ttatgccaaa ctcttttgcc tacaatgatg atgtaaaacc gataacacaa gatttattaa
     2641 aagcaaaagc tttgttaaaa gaggctggat atgatgaaaa aaatccttta gtttttgaag
     2701 tggttactaa tacaggaaat gatataagag taaacactgc acaaatcctg cagtatcaac
     2761 tctcaaaaat tggtgtccag atgaaaataa gagttatgga gtggcaagca tttttaaata
     2821 cagttgttca tccacgaaaa tttgaaactg tacttttagg atggtcttta gctttgatgc
     2881 cagatgctta tcctttgtgg catagcaaaa gtgcgaaact aggtggattt aacctagtaa
     2941 attacaaaaa tgaaaaggtt gatgaattaa tagaaaaagg aagtggaact ataaataaag
     3001 atgaacttgg tgcaatttat aaagagattt ttaaaattgt tgccaatgat ttgccatatc
     3061 tatttttgta tattccagat ggaataactg ctataaataa aaaaatagaa aatattgatc
     3121 cagcatttat aggtattatg cacaatcaaa aagattggga aataaaagag taaggaagat
     3181 ttttatgaac aaaaaagata agattatttt atttgattta gatggtactt taatagattc
     3241 aacagatgca attaccgata catttttaca tgctttttca aaaca
//
LOCUS       DAOQAN010000060         3255 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_40063_length_3255_cov_3.233437, whole genome shotgun sequence.
ACCESSION   DAOQAN010000060 DAOQAN010000000
VERSION     DAOQAN010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 3255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3255
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40063_length_3255_cov_3.233437"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="PKO10_01610"
     CDS             <1..247
                     /locus_tag="PKO10_01610"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNW65891.1"
                     /translation="KIIKDEADIIFTTAHKAKGMEYNQVIMTDDFITKKDILNRKKNL
                     SFASICEELNIYYVAASRAKFSISLADLKLDYKEEKD"
     gene            333..1082
                     /locus_tag="PKO10_01615"
     CDS             333..1082
                     /locus_tag="PKO10_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW65892.1"
                     /translation="MQYFGEIQTKYEEVFLTQSYMYFKKDEDENISAKDLKNLIKTKD
                     DRKKNIIGTVFIYNPVVTPIGFDSNKFLLEQDFKAFDELIELKCEGYITIFKQAMKDS
                     CKGKIVEIKNLFNLIEGHIDSSTLLSKFNEDLEKYNSNQNTEFDRDIMYLDAQNYLPF
                     GKFVYFCWGDKINPKEFSNISDYARTIYENCVKSGKKIAFAYKKEKTVDESIKYLQFS
                     NPMQNYKNKNSISFAIKRSFDTFPPTPAFYE"
     gene            1154..1228
                     /locus_tag="PKO10_01620"
     tRNA            1154..1228
                     /locus_tag="PKO10_01620"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1186..1188,aa:Gln,seq:ttg)
     gene            1251..1335
                     /locus_tag="PKO10_01625"
     tRNA            1251..1335
                     /locus_tag="PKO10_01625"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1285..1287,aa:Tyr,seq:gta)
     gene            1353..1428
                     /locus_tag="PKO10_01630"
     tRNA            1353..1428
                     /locus_tag="PKO10_01630"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1386..1388,aa:Phe,seq:gaa)
     gene            1503..2012
                     /locus_tag="PKO10_01635"
     CDS             1503..2012
                     /locus_tag="PKO10_01635"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNW65893.1"
                     /translation="MSKIGIIVASSNNNQKLAIELQKIAIELNYDSEIINLVDYNLPL
                     YSTIEEEKNGIPESVLDLATRIIDLKAFIIVAPEYNGVMPPVLNNAMAWTSRATKNWR
                     DAFDDKIVALATHSGGGGQKGLQAMRIMFQHLGANILAREILTTYDKPLNKNSAIAIL
                     QNLTKLSRV"
     gene            complement(2043..>3255)
                     /locus_tag="PKO10_01640"
     CDS             complement(2043..>3255)
                     /locus_tag="PKO10_01640"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing phosphodiesterase"
                     /protein_id="HNW65894.1"
                     /translation="NRILREDYLTEIGSRYKLIEDISKQENLSISILDIKNFSEVNDF
                     FGYKIGDYILKLLAKRIEELVIKKPKYGVYRLTSDVFAILASNEQKSDFIKNIEEISK
                     IISSKAVRAKGREIFVSLNYSFSFEPKSSLLETANVMRKYTKSNPNQIIYDRSLEIEK
                     DYEKNIFWTLKIKKALENNDIIPYYQAIYNLQTNKIEKYEALMRLNDSGKIVSPFFFL
                     DIAKKSGQYLQLTKRMIDSSFEYFKDKDFEFSINFTFQDIANEEISNYVIEKIKELNI
                     GNRVVFEIVESEEIDDFNLINGFFSTIRKLGCKIAIDDFGSGYSNFEYLIKLDADYIK
                     IDGSLIKDVLVSKGNENIITMIINFAKDQGFKTIAEFVSSKEILEKVKYLGVDYVQGY
                     YIHEPSDKIED"
BASE COUNT         1227 a          460 c          408 g         1160 t
ORIGIN      
        1 aaaaataata aaagatgaag cagatattat cttcacaaca gcacataaag caaaaggtat
       61 ggagtataat caagtaatta tgacagatga tttcatcaca aaaaaagata tcttaaatag
      121 aaaaaaaaat cttagttttg cttcaatttg tgaagagttg aatatatatt atgtagcagc
      181 ttcaagagcc aaattttcta taagtttagc tgatttaaaa cttgattaca aagaagaaaa
      241 agattaaaaa tagtattttt ataaattata gaaaatgaaa tatgatataa tttccgactt
      301 aaaaactata gcaaaaaaat aaaaggataa atatgcagta ctttggagag atacaaacaa
      361 aatatgaaga ggtattttta acacaaagtt atatgtattt caaaaaagat gaagatgaaa
      421 atattagtgc aaaagattta aaaaatttaa taaaaacaaa agatgataga aaaaaaaata
      481 ttataggaac tgtatttata tataacccag ttgttacacc tattggattt gattcaaata
      541 aatttttact agagcaagat ttcaaagctt ttgatgaatt aatagaacta aaatgtgaag
      601 gttatataac tattttcaag caagctatga aagatagttg caaaggaaaa atagtagaga
      661 taaaaaatct atttaatcta atagaaggac acattgactc aagcactctt ttatcaaaat
      721 ttaatgaaga tttagaaaaa tataactcaa atcaaaatac agaatttgat agagatatta
      781 tgtatttaga tgctcaaaat tatcttcctt ttggaaaatt tgtctatttt tgttggggtg
      841 ataaaataaa tccaaaagag ttctcaaata tatcagatta tgcaagaact atctatgaaa
      901 attgtgtaaa aagcgggaaa aaaatagcat ttgcttacaa aaaagagaaa actgttgatg
      961 aatcaataaa atatcttcaa ttctcaaatc ctatgcaaaa ttataaaaat aagaattcaa
     1021 tctcttttgc tattaaaagg tcttttgata cttttcctcc tactcctgct ttttatgaat
     1081 aatattcata aaaagcatat tttttagcta attttaataa ttaagattat acaataccgt
     1141 ccacttaaat taatggggta tcgccaagcg gtaaggcaac ggtttttggt accgtcactc
     1201 gaaggttcga atccttctac cccatccaaa aatttaagtt cttttattat ggtgaggttg
     1261 gagagtggtc aaatcctgcg gactgtaaat ccgccgccta cggcttcgaa ggttcaaatc
     1321 cttctctcac caccactttt aaaatatatc atggctcgat agctcagtcg gtagagcaaa
     1381 ggattgaaaa tccttgtgtc gacagttcga ttctgtctcg agccaccact tcaaaaaatc
     1441 acaaatcata tttaaataat cattagctat aatatttctt tacaatatta ttaaggattt
     1501 ttatgtcaaa aattggaata attgttgcta gttcaaacaa caaccaaaaa ctagctattg
     1561 aacttcaaaa aatagcaatt gagctaaact atgatagtga aattataaat ttagttgatt
     1621 acaatctgcc tctttatagc acaattgaag aggaaaaaaa tggaattcct gaatctgttt
     1681 tagatttagc tacacgaata attgatttaa aagcatttat tattgtagct cctgagtata
     1741 atggtgttat gccacctgtt ttaaataatg caatggcttg gacatcaaga gctacaaaaa
     1801 actggagaga tgcttttgat gataagatag tggcattagc aacacacagt ggtggtggag
     1861 gtcaaaaagg tcttcaagct atgagaatta tgtttcaaca tttaggtgca aatattttag
     1921 caagagagat tttaacaact tatgataaac ctctaaacaa aaatagtgct atagcaatct
     1981 tacaaaatct tacaaaacta tcaagagtat agggatttta tccttatgct aatttgctta
     2041 ttttaatctt ctattttatc gctaggttcg tgtatataat aaccttgaac ataatcgaca
     2101 cccaaatatt taactttttc taaaatctct ttacttgata caaactctgc aatagtttta
     2161 aatccttgat ctttagcaaa atttataatc atagtaatta tattttcatt tcctttgctt
     2221 actaatacat ctttgattaa agagccatca attttaatat aatcagcatc tagtttaatc
     2281 aagtactcaa aattcgagta tccacttcca aaatcatcta ttgcgatttt acaacctaat
     2341 tttctaatag tagaaaaaaa tccatttatt agattaaaat catcaatctc ttcactctca
     2401 acaatttcaa aaacaactct attaccaata tttaactctt ttattttttc tattacatag
     2461 ttagaaattt cttcgtttgc aatatcttga aaagtaaaat ttatagaaaa ttcaaaatct
     2521 ttatctttga aatattcaaa actagaatca atcattcttt ttgttagttg taaatattgc
     2581 ccagattttt ttgcaatatc taaaaagaaa aatggagata caatttttcc agaatcattt
     2641 aatctcatta aagcttcata tttttctatt ttatttgttt gcaaattata tattgcttga
     2701 taatatggaa taatatcgtt attttctaaa gcttttttaa tctttaatgt ccaaaaaata
     2761 ttcttttcat aatctttttc tatctctaaa cttctatcat aaataatctg atttggattt
     2821 gattttgtat attttctcat aacatttgca gtttcaagaa gtgaactttt tggctcaaaa
     2881 gagaagctat aattaagaga aacaaatatc tctctacctt ttgctcgaac tgcttttgaa
     2941 gatattattt ttgaaatctc ttctatattt tttataaaat cacttttttg ttcatttgaa
     3001 gctaatattg caaaaacatc agatgttagc ctataaacac catattttgg cttttttata
     3061 accaattctt ctattctttt agcaagtaat tttaaaatat aatctcctat tttgtagcca
     3121 aaaaagtcat ttacctcact aaaatttttt atatctaata ttgatatact taagttctct
     3181 tgtttactta tatcttcaat aagtttatat ctacttccta tttcagtcag ataatcttct
     3241 cttaaaattc tattt
//
LOCUS       DAOQAN010000061         3213 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_40858_length_3213_cov_2.478784, whole genome shotgun sequence.
ACCESSION   DAOQAN010000061 DAOQAN010000000
VERSION     DAOQAN010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3213
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40858_length_3213_cov_2.478784"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            82..354
                     /locus_tag="PKO10_01645"
     CDS             82..354
                     /locus_tag="PKO10_01645"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW65895.1"
                     /translation="MLIGLLFINCGHKVIKAINTKRSYFAHTSNKLLPKEKYMIGVKT
                     GYTSKAGPCLIARAKEGKKDILLVMLNSQNRWANTKLALDTVLDKR"
     gene            complement(359..856)
                     /locus_tag="PKO10_01650"
     CDS             complement(359..856)
                     /locus_tag="PKO10_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014472940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW65896.1"
                     /translation="MNREKNIKNYILNYIYATSKQPILLKDMLVASVQYRNDMEVDSS
                     RLGFRLRLTRAYLVYVWLVLAVLLPISLLTHKLLAKIDAHISIVGGMIITALIFMGFN
                     YFKDIVKKEMTKSRLKKAWSLHFPFFDYEEYANKVNEIFEEAMREEVSKRDLQKYILD
                     RLTNI"
     gene            1015..1635
                     /locus_tag="PKO10_01655"
                     /pseudo
     CDS             1015..1635
                     /locus_tag="PKO10_01655"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            1954..>3213
                     /locus_tag="PKO10_01660"
     CDS             1954..>3213
                     /locus_tag="PKO10_01660"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HNW65897.1"
                     /translation="MPFEATLNNKHIGIIEEYLVEISKNLDIELNFVPTLNWHESFEK
                     VVENKCDILTTIADKKNREKLFNFTNPYLDFPFVIATDISKPFIEDLKTLKRVKIALV
                     KDFATSNIIKNRYKNINFVEYPNLYEALEAVRKGEVYAAIDSLAVIGYEIQNHFVGDI
                     KVSGKIDENLKLYMATNIENKTLASILNKALNSINENQKHEFFNKWVYINYQNSIDKD
                     AVLKLFILILAIFFILAIIYRAYLLKKTNQELENRVAFEIKQNEEKNGILLQQSKMAA
                     MGEMLENIAHQWRQPLSTIGICTSGMELKKNLNMLEDKDFYDSINHIKSSISYLSNTI
                     EDFRSFLNKDKVLSNVNINHLVQKVLDILNPSLQSHHIEVIKNIDDFEFSTIENDLIQ
                     ILMNIITNAKDALKDLKDEEPRYIFINI"
BASE COUNT         1287 a          378 c          428 g         1120 t
ORIGIN      
        1 aaataccacg gttaaaaata tttctatcta caataaattt aatttaaaag aatttgaaat
       61 ttatcgataa tatagggacg attgttaata ggtttattat ttataaactg tgggcataaa
      121 gttattaaag caataaatac aaaaagatct tactttgcac atactagtaa caaacttctt
      181 ccaaaagaga agtatatgat tggagttaaa actggttata caagtaaagc tggaccttgt
      241 ttaattgcaa gagcaaaaga agggaaaaaa gatattttgc tagtaatgct aaattctcaa
      301 aatagatggg caaatacaaa actagcttta gatacagttt tagataaaag atagattact
      361 aaatattagt caatctatct aaaatatatt tttgtaaatc tctttttgaa acctcttctc
      421 tcatagcctc ttcaaaaatc tcatttactt tattagcata ctcttcataa tcaaaaaaag
      481 ggaaatgtaa actccaagcc ttttttaatc tactttttgt catctctttt tttacaatat
      541 ctttaaaata gttaaatccc ataaatataa gtgctgtgat aatcatccca ccaacaattg
      601 atatatgtgc atctattttt gccaacaatt tatgcgtaag aagagaaata ggtagaagaa
      661 ctgctaaaac taaccaaaca taaacaagat aagcccttgt taatctcagt ctaaatccta
      721 atcttgaact atcaacttcc atatcatttc tgtattgaac tgaagcaacc aacatatctt
      781 ttaataaaat tggttgtttt gaagttgcat aaatataatt caatatgtag tttttaatat
      841 ttttttctct attcataata tttcctcaaa taaattgtca caattctatc ttagtttgct
      901 ataataattg ttaaaatttt aaagctaagg tggattttga aaaagatatt tattcttatt
      961 ttacttttca aatacctata ttctacagat ttacaaaaag tttcgcttca acttatgtgg
     1021 cttgaccagt tccagtttgc tggattttat atagcaaaag agaaaggctt ttatcaaaaa
     1081 gttggacttg atgttgagtt taaaaaattt caaaatgaaa caaatgtgtt gcaagaagtt
     1141 actcaaaata gagcaacttt tggacttgct tctagctctt taatagtgca taaatcaaat
     1201 ggtgcagata tatctatatt tggagctatt tttcaaactt cacctcttgt gattttggct
     1261 ttaaaaaatt caaatataga ctctattaaa gatttaaaag ataaaaactt aatgattgca
     1321 gatgagtagc taaattttgc aaatttacaa gctatgttta gtagtcaaaa ctttgatatt
     1381 aaatccgtaa aactaattcc tcactctttt aatgttgatg atttaataaa taaaaaagct
     1441 gatttaatgg cttcttatac tacaaatgaa ccttttttac taaaagaaaa aggttatgaa
     1501 agcaaaattt tctacccaaa agattatgga tttgactttt atgaaaatat tatgtttaca
     1561 aatagtgaat ttgcagaaaa aaacccaaaa attgtatatg atttttataa agcttcaata
     1621 gatggttgga aatgggcatt tgaaaatatt gaaaaaagtg ttgatattgt ttttgaaaaa
     1681 tataatccac taaataaaag taaagaggca ctactttttg aagcaaatga gataaagaaa
     1741 attatttttg ataaaaacgg agatattgga acaatttcta atgaaaaaat gaacctaata
     1801 ttaaatacat ataaggttat gggtttaata aaaaatgata tagatttaaa aagttttgtt
     1861 ttacataata gttttcaaaa tgaactaaat agtgaagaaa aagagtattt aaaaacaaaa
     1921 aaatctttta cttattgttc tcataacgac ttaatgccat ttgaagctac tttaaataat
     1981 aaacatattg gaattataga agagtatctt gtagaaattt caaaaaattt agatatagaa
     2041 ttaaattttg taccaacttt aaattggcat gaaagttttg aaaaagttgt agaaaataag
     2101 tgtgatatct taacaacaat tgcagataag aaaaatagag aaaagctttt taattttaca
     2161 aacccttatc tggattttcc ttttgtgatt gctacagata tttctaaacc ttttattgaa
     2221 gatttaaaaa ctctaaaaag agttaaaatt gctcttgtga aagattttgc aacaagcaat
     2281 ataataaaaa atagatataa aaatatcaat ttcgtagagt atccaaatct ttatgaagct
     2341 ttagaagctg taagaaaagg tgaagtttat gctgctattg actctcttgc tgttattgga
     2401 tatgagatac aaaatcattt tgtaggagat atcaaagtat ctgggaaaat agatgaaaat
     2461 ctaaaacttt atatggcaac aaatattgaa aataaaactc ttgcctcgat tttaaataag
     2521 gctttgaatt caataaacga aaatcagaaa catgaatttt ttaataaatg ggtatatata
     2581 aactatcaaa atagtattga taaagatgca gttcttaagt tatttatttt aattttagct
     2641 atatttttta ttctagctat tatttataga gcttatttac tcaaaaaaac aaatcaagaa
     2701 ttagaaaata gagttgcttt tgagataaaa caaaatgaag agaaaaatgg aattctttta
     2761 caacaatcaa aaatggcagc aatgggtgaa atgcttgaaa atatagctca tcaatggaga
     2821 caaccactat caactattgg tatttgtact tcaggaatgg agcttaaaaa aaatctaaat
     2881 atgctagagg ataaagattt ctatgattca ataaatcata taaaatcttc tatatcttac
     2941 ttaagcaata ctattgaaga ttttagaagc ttcttaaata aagataaagt tttatctaat
     3001 gtaaatataa accatttagt acaaaaagta ctcgatattt taaatccatc tttacaatct
     3061 catcatattg aagttataaa aaatatagat gattttgagt tttcaactat tgaaaatgat
     3121 ttaattcaaa ttttgatgaa tattattaca aatgcaaaag atgccttgaa agatttaaaa
     3181 gatgaagagc caagatatat ttttattaat att
//
LOCUS       DAOQAN010000062         3207 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_40967_length_3207_cov_3.585977, whole genome shotgun sequence.
ACCESSION   DAOQAN010000062 DAOQAN010000000
VERSION     DAOQAN010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 3207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3207
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40967_length_3207_cov_3.585977"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="PKO10_01665"
     CDS             <1..540
                     /locus_tag="PKO10_01665"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HNW65898.1"
                     /translation="IYTYPMKGTIDTNLTDAKNILLNSKKELAEHTMVVDLLRNDLGK
                     VANNIKVEEFRKFSKIVTKDRELFQTSSVISGDLQNNWQEKIGDILSNILPAGSITGT
                     PKKSTIEILKNIENYDRGFYSGIFGIFDGINLQSFVLIRFIENINNELFYKSGGGTTS
                     DSIAKEEYEELLNKVYLPF"
     gene            550..1110
                     /locus_tag="PKO10_01670"
     CDS             550..1110
                     /locus_tag="PKO10_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV family protein"
                     /protein_id="HNW65899.1"
                     /translation="MFFETIKCEDFEIFNLEYHQKRVAKTIGKNFDLQEYINPPTNEL
                     LRCKIVYNKDEILSVDYFPYKIRDIKSFKIVVDNSLDYSKKYLNRDKLDNLFLKKDSC
                     DEIIILKNGVVTDTTIANIAIFYDGVWITSKNCLLEGTSRAKLIEKKEIFVKDISLDM
                     LKKASKIALMNAMIDFYEIKDFRIEY"
     gene            complement(1161..2258)
                     /locus_tag="PKO10_01675"
     CDS             complement(1161..2258)
                     /locus_tag="PKO10_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014467991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HNW65900.1"
                     /translation="MQKVYIYLENGIFLEANSFGADTTAVGKLVYNNSTFGQQEIITD
                     PSNNGLFINFTAVEIGNTGANRVDMESSKAHAKGIIVRNYHDAYSNYRAEMSLKDFLV
                     EQNVIGICDIDTRFLTKIVREEGSMMMIASTQISSKDELAKKLNEAKKYDEINFVKDI
                     STKEAYIHKSGVWNHETGEYNKAQMSDKRVQVIDYGVKKSFLNELVELGFEVEVVPAS
                     TKADDIINNFKAGKIGGVVLSSGAGNPNILTDEIAEIKRLIDANIPILAVGLGHYLLA
                     LASGVKVDKIKSIKYGSHPIRGEKTVEICGINGDFKISEDIKNIADVTHIKVFNDSAV
                     ALKYKNKNELSSEFSPVSNSSICQEFSQMVK"
     gene            complement(2258..2809)
                     /locus_tag="PKO10_01680"
     CDS             complement(2258..2809)
                     /locus_tag="PKO10_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014472917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF507 family protein"
                     /protein_id="HNW65901.1"
                     /translation="MRLKLHQTTYLSRRITRDLITCDFIEARKDKASIEEQVERILDE
                     DILKEQSLDEKVEEILDSQTEEIEYLNADRRQLFWMTKKRLANDFGVILNNEDRFSDI
                     AHKILDYLWEEDYIHFTCSDNQIKNVIFGSLDDFIKGFEKADGEVLAKLKNYKRKLIP
                     GTEDYDLVYHRLYEEELIKRGLI"
     gene            complement(2811..>3207)
                     /locus_tag="PKO10_01685"
     CDS             complement(2811..>3207)
                     /locus_tag="PKO10_01685"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HNW65902.1"
                     /translation="GEVGKEFGAVTGRKRRCGWFDAVAVRYASRLNGCDKLALMKLDV
                     LDGFDKIKVCVAYNYNGERIDYMPSNMDNVEAIYEEIDGWDSVVGIRKYEDLPINAKK
                     YIEKIEEITNVKVGIISTSPERDDTILRG"
BASE COUNT         1114 a          512 c          375 g         1206 t
ORIGIN      
        1 atctatacat atcctatgaa aggaactatt gatacaaatt taactgatgc aaaaaatatt
       61 ttactaaata gtaaaaaaga gttagcagaa cacactatgg ttgttgattt gctaagaaat
      121 gatttaggaa aagttgctaa taatataaaa gttgaagagt ttagaaaatt tagcaaaata
      181 gttacaaaag atagagaact ttttcaaaca agctcagtta tttctggaga tttacaaaat
      241 aattggcaag aaaaaatagg agatatttta tcaaatattc ttcctgctgg aagtatcaca
      301 ggtactccaa aaaaatctac aatagaaatt ttaaaaaata ttgaaaacta tgatagagga
      361 ttttatagtg gaatttttgg tatttttgat ggtataaatc ttcaaagttt tgtgctaatt
      421 agatttatcg aaaatataaa taatgagctt ttttataaaa gtggcggtgg gactacaagt
      481 gatagtattg caaaagaaga gtatgaagaa cttttaaata aagtttatct accattttaa
      541 aggaaatata tgttttttga aacaataaaa tgtgaagatt ttgagatttt taatttagag
      601 tatcatcaaa aaagagttgc aaaaactatt ggaaagaatt ttgatttaca agagtatata
      661 aatccaccaa caaatgagct actaagatgt aagatagttt acaacaaaga tgaaatttta
      721 agtgtagatt attttccata taaaatacga gatataaaaa gctttaaaat agtagttgat
      781 aacagtttag attactcaaa aaaatatcta aatagagata aacttgacaa cttattttta
      841 aaaaaagata gttgtgatga gataattatt cttaaaaatg gagtagttac ggatacaaca
      901 attgcaaata tagctatatt ttatgatgga gtttggatta cttcaaaaaa ttgtttgtta
      961 gaaggaactt caagagcaaa attaatagaa aaaaaagaga tatttgtaaa agatataagt
     1021 ctggatatgt taaaaaaagc ttcaaaaata gctcttatga atgctatgat tgatttttat
     1081 gagataaagg attttagaat agagtattaa aaaattaaat tatttttatt actgaggcaa
     1141 agcctcagat atttttaact ctattttacc atttgcgaaa actcttggca aatactagaa
     1201 tttgaaactg gagagaattc actacttaat tcatttttat ttttatattt taaagctaca
     1261 gcactgtcat taaaaacttt aatatgagtt acatctgcaa tgtttttaat atcttcactt
     1321 attttaaaat caccattaat accacaaatt tctactgttt tttcacctct aataggatgg
     1381 cttccatact ttatagactt aatcttatct acttttacac cacttgcaag tgctagtaaa
     1441 tagtgtccta gaccaactgc aagaatcgga atatttgcgt ctattaatct ttttatttca
     1501 gctatttcat cagttaaaat atttggattt ccagctccac tacttagaac tactccacct
     1561 attttacctg ctttaaaatt attaattata tcatctgctt ttgtagaagc aggaactact
     1621 tcaacttcaa atcctaactc aacaagctca tttaaaaatg attttttaac tccataatca
     1681 attacttgaa ctcttttatc actcatttga gctttattat actctcctgt ttcatgattc
     1741 caaacacctg atttatgaat ataagcctct ttagttgaaa tatcttttac aaaattgatt
     1801 tcatcatatt tttttgcttc atttaatttt tttgctagtt catctttaga agagatttgt
     1861 gtagatgcaa tcatcatcat acttccctct tctcttacaa tttttgtcaa gaatcttgta
     1921 tcaatatcac aaattccaat aacattttgt tctactaaaa aatcttttag actcatttct
     1981 gctctataat ttgagtaagc atcatgataa tttctcacaa ttataccttt tgcatgagct
     2041 tttgaacttt ccatatcaac tctatttgca cccgtatttc ctatctctac agctgtaaaa
     2101 ttgataaata aaccattatt tgaagggtcc gtaataatct cttgttgtcc aaatgttgag
     2161 ttattataaa ccagttttcc aacagcagtt gtatctgcac caaaagagtt tgcctctaaa
     2221 aatattccgt tttctaaata aatatatact ttttgcatca aattaatcct ctttttatta
     2281 actcttcttc ataaagtcta tgataaacca aatcatagtc ctctgtacct ggaattagct
     2341 ttcttttata gtttttaagt ttagctaata cttcaccatc tgctttttca aaccctttaa
     2401 tgaaatcatc caaagaacca aatatcacat tttttatttg gttatctgaa caagtaaaat
     2461 gtatataatc ctcctcccat agataatcta atattttgtg agcaatatca gaaaatctat
     2521 cttcattgtt taagataact ccaaaatcat ttgcaagtct tttttttgtc atccagaaaa
     2581 gttgtcttct atcagcattt agatactcta tctcttctgt ttgagaatct aaaatctctt
     2641 ctactttctc atctaaagat tgctctttta gaatatcttc atctaaaatt ctttcaactt
     2701 gctcttctat actggcttta tcttttcttg cctctataaa atcacaagtt attaaatctc
     2761 ttgtaattct tcttgaaagg tatgtagttt ggtgtagctt taatctcatt ttatcccctt
     2821 aaaattgtat catctcgttc tggtgaagtt gaaattattc caacctttac atttgttatc
     2881 tcttcaattt tttctatata tttttttgca tttattggta aatcttcata ttttctaatt
     2941 ccaacaacac tatcccaacc atctatttct tcataaatag cttctacatt atccatattt
     3001 gatggcatat aatcaattct ctctccatta tagttatatg caacacaaac tttaatttta
     3061 tcaaatccat ctagtacatc tagcttcata agtgctaatt tatcacaccc atttaatcta
     3121 cttgcatatc taactgcaac agcatcaaac caaccacatc ttctttttct tccagtcact
     3181 gcaccaaact ctttcccaac ttcaccc
//
LOCUS       DAOQAN010000063         3121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_42644_length_3121_cov_2.781148, whole genome shotgun sequence.
ACCESSION   DAOQAN010000063 DAOQAN010000000
VERSION     DAOQAN010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42644_length_3121_cov_2.781148"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="PKO10_01690"
     CDS             complement(<1..294)
                     /locus_tag="PKO10_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW65903.1"
                     /translation="MKKLLFIFFSFTFLNASDIQKAFLIAVYDKNRVENVQHKIKTDF
                     QYRGEVFFKVAIIGNYNKNVNVTTKINNSNGKLINTETLYNNLTKKIYGYELTF"
     gene            complement(291..1916)
                     /locus_tag="PKO10_01695"
     CDS             complement(291..1916)
                     /locus_tag="PKO10_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNW65904.1"
                     /translation="MKKNTINSDTLLSLISKHSPDMLWIKDLNGRYLYANNAICNGLL
                     IATPDEVIGKTDIFFALREREKYKDNKNWHTFGELCTNTDLETIKHMRPLRFLENGNI
                     QGKMTYLEVDKAPFFDENKNLIGVIGTARDITEQILLKERNEYLMYFDQLTSLPNRQK
                     IILDIENKKAKSSIVFNIDDFKEVNDFFGTNNADKILQDIALRFIKYGYTAYRIDGDE
                     FAILFFDEKSEEELQNEAIKIIDLLDSEPFYIDDKTILITFSIGIAKSDEKLLTKVDI
                     AVNNAKTSNNHICIYEESKNIEERYKENIELATQIKEAILGNRIICHYQPLLNIENSE
                     IYSYESLVRLIDNKGNSLAPYKFLDFSKKIKLYSSITKIVVTQACKTFQNRDENFSIN
                     LSVDDIKDLETVDFILKTIKETNTSRRVTFEILESEGIENYQEVISFINQIKSLGARI
                     AIDDFGTGYSNFEHLLRLDVDYIKIDGSLIKNIVTDDKHRLIVETIVSFARRIGIKTV
                     AEFVSDEKILQTIKEIGISCAQGYHIGKPETLV"
     gene            complement(2006..2392)
                     /locus_tag="PKO10_01700"
     CDS             complement(2006..2392)
                     /locus_tag="PKO10_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HNW65905.1"
                     /translation="MNISSIVVQTLPKYLDSVIENLKKSGVCDYHMHDEKGRIIITIE
                     GKNVEEELKKLKVIEAIPYVSSADMQMSYSEEELSNHMEVLENADLVPKVLNDKDIKV
                     EDIVYRGDLKRKDLIGFAKEFDNSKK"
     gene            complement(2395..>3121)
                     /locus_tag="PKO10_01705"
     CDS             complement(2395..>3121)
                     /locus_tag="PKO10_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HNW65906.1"
                     /translation="VVLYKCSILSFDDKILILSQGQSGGRDIFISKNGILENIISSDE
                     RLFIAYAKFLDNNKIVYALLSNQIFIYDLINKKILKSTQISQSSFSHFVLSSDKKSIF
                     VADESGIISELNTSNLEKIGQFKGQNVDRVFQVDFKENTILACGQDRRASIYYKNQKK
                     PYYKSIDFLVYAGALDDKAKKAAISLNEDNDVLVFDVDTKEELFILKGNNALITSILF
                     LDENSLVVASDDKKVNIYNLKKD"
BASE COUNT         1050 a          466 c          290 g         1315 t
ORIGIN      
        1 aaaagttagt tcatatccat atattttctt tgttaaatta ttgtaaagag tttctgtatt
       61 tattaatttt ccatttgagt tatttatttt tgttgttaca tttacatttt tattgtaatt
      121 tcctattatg gcaactttga agaaaacttc acctctatat tgaaaatctg tttttatttt
      181 atgttgcaca ttttcaactc tatttttatc ataaactgct attaaaaatg ctttttgtat
      241 atcgcttgca tttaaaaaag taaaactaaa gaaaataaat aataattttt tcataccaaa
      301 gtttctggtt ttcctatatg ataaccttgt gcacaactaa ttcctatctc ttttattgtt
      361 tgtaatatct tttcatcact tacaaattca gcaactgttt taattccaat tcttcttgca
      421 aaactaacta ttgtttcaac aattaatcta tgtttatcat ctgtaactat attttttatt
      481 aaagaaccat caattttaat ataatcaaca tctaatctta aaagatgttc aaaatttgaa
      541 tatcccgttc caaaatcatc tatagcaatt cttgcaccca aagattttat ttgattgata
      601 aaagagataa cttcttgata gttttctata ccttctgact ctaaaatctc aaaagttact
      661 cttcttgaag tgtttgtctc ttttatagtt tttaatataa aatcaacagt ttctaaatct
      721 tttatatcat caacgcttaa atttatagag aagttttcat ctctattttg gaatgtttta
      781 caagcttggg ttacaactat ttttgtgata cttgagtata gttttatttt tttagaaaaa
      841 tctagaaatt tatatggagc aagagagttt cctttgttat ctataagcct tactaaactc
      901 tcataagagt aaatttcact attttctata tttaaaagag gttgataatg acaaataatt
      961 ctatttccta aaattgcctc ttttatctga gttgcaagtt caatattctc tttatatctc
     1021 tcttctatat ttttactctc ttcgtaaata cagatatgat tatttgaagt ttttgcatta
     1081 tttaccgcaa tatcaacttt tgttaaaagt ttttcatcac tttttgcaat accaatagaa
     1141 aatgtaatta aaattgtttt atcatcaata taaaaaggtt cactatctaa taaatctata
     1201 attttaattg cttcattttg taactcttct tctgattttt catcaaaaaa taaaatagca
     1261 aattcatcac catctattct atatgctgtg tatccatatt ttataaatct taaagctata
     1321 tcttgtaata ttttatcggc attatttgta ccaaaaaaat catttacctc tttaaagtca
     1381 tcaatattaa aaacaataga agatttagct tttttatttt caatatctaa aataattttt
     1441 tgtctatttg gaagagatgt tagttggtca aaatacataa gatattcatt tctctctttt
     1501 aaaagaatct gttctgtaat atctcttgca gttcctatta caccaattaa atttttattt
     1561 tcatcaaaaa atggagcttt atcaacttca agatatgtca ttttcccttg aatattccca
     1621 ttttctaaaa atcttaatgg tctcatatgc tttatcgttt ctagatcagt attagtacat
     1681 aactctccaa atgtatgcca atttttatta tctttatatt tttctctttc tcttaaagca
     1741 aaaaagatat cagtttttcc aataacttca tcaggagttg caattagtaa accattacaa
     1801 atagcattat ttgcatataa ataacgacca tttaaatctt ttatccataa catatcagga
     1861 gagtgtttag aaattaagct aagaagagta tccgagttta tagtgttttt tttcataatc
     1921 cctcaaattg aatagttgaa ggattatatc ttaagttatt aaaaataact ttaaggaata
     1981 tttaataatt attttctttt aagtattatt ttttactatt atcaaactct tttgcaaaac
     2041 ctattaaatc ttttcttttt aaatcacctc tataaactat atcttcaact tttatatctt
     2101 tatcatttaa aacttttgga actaaatcag cattttctaa aacttccata tgatttgata
     2161 gctcttcttc actataactc atctgcatat cagcactact tacataagga attgcctcaa
     2221 ttacttttag tttttttagc tcctcttcta catttttacc ttcaattgta ataataattc
     2281 tacctttttc atcatgcata tgataatcac aaacaccaga ttttttaaga ttttctatca
     2341 cactatcaag atattttggt agagtttgta caacaatact tgaaatattc atttttaatc
     2401 cttttttaaa ttatatatat ttactttttt atcatcactt gctacaacta aactattttc
     2461 atctaaaaac aaaatagatg taataagtgc attatttcct tttaggataa aaagctcttc
     2521 ttttgtatct acatcaaaaa ctaaaacatc attatcttca tttaatgaaa ttgctgcttt
     2581 ttttgcttta tcatctaaag ctcctgcata aactaaaaaa tcaattgatt tgtagtatgg
     2641 ctttttttga tttttgtaat aaatagatgc tcttctatct tgtccgcaag ctaaaatagt
     2701 attttctttg aaatcaactt gaaaaactct atctacattt tgacctttaa attgtccaat
     2761 tttttctaag tttgaagtgt tgagttctga aataattcca ctttcatcag ctacaaaaat
     2821 agatttttta tcactactta aaacaaaatg agaaaaagat gattgagaaa tttgagttga
     2881 ttttaagatt tttttattta ttaaatcata tataaatatc tgatttgata aaagagcata
     2941 gactatttta ttattatcta agaattttgc ataagctata aaaagtctct catctgaact
     3001 aattatattt tctaaaatac cattttttga tataaaaata tctcttcctc cactttgtcc
     3061 ttgtgataaa attaaaatct tatcatcaaa acttagaatt gaacatttgt ataaaacaac
     3121 t
//
LOCUS       DAOQAN010000064         3111 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_42834_length_3111_cov_3.001963, whole genome shotgun sequence.
ACCESSION   DAOQAN010000064 DAOQAN010000000
VERSION     DAOQAN010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3111
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42834_length_3111_cov_3.001963"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2585)
                     /gene="acnD"
                     /locus_tag="PKO10_01710"
     CDS             complement(<1..2585)
                     /gene="acnD"
                     /locus_tag="PKO10_01710"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013136522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047456 - 2-methylisocitrate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe/S-dependent 2-methylisocitrate dehydratase
                     AcnD"
                     /protein_id="HNW65907.1"
                     /translation="MTNEKYLKQLDGLDTKYYDVKSAVEDITPGSFAKLNYTSRVLAE
                     NLLRKCPSEDLKDSLIQLIEKRTDKDFPWYPSRVICHDILGLTAFVDLAGLREAVASK
                     GVDPQKINPVVPTQLIVDHSLAVECGGFDPDAFQKNRDIEDRRNADRFDFINWTKKAF
                     DNVDVIPPGNGIMHQINLEKMSPVIHNIDGIASPDTLVGTDSHTPHVDALGVIAVGVG
                     GLEAENVMLGNPSYMRVPEIIGVEITGTRGAGITATDIALSLTSFLRDNNVISAYLEF
                     YGSGIKYLDLGDRATISNMTPEYGASAAMFAIDDRTIDYLRITGRSPEQVKLVETYAK
                     ANGLWADTLSEATYARTIKFDLTTVTRTLAGPSQPHKLLPVSALDDRGIAKKIEITND
                     VMPDGAVLIAAITSCTNTSNPRNVVAAGLLAKKANELGLTRKRWVKSSLAPGSKVSEI
                     YLKDSGLLGELEKLGFGIVGFACTTCNGMSGALDPKIEAEATASGVYTTAVLSGNRNF
                     DGRIHPFIKEAFLASPPLVVAYALAGSIRVNIETGVIGIDKNGNEVRLKDLWPSDEEI
                     DAIVYKSVKPEMFAKIYDPMFAKSDKGAKAEPFYKWDAKSTYIQKPPYWEDAFMSMPA
                     LKNLRPLGIFPNDITTDHLSPSNAIQANSASGEYCLKMGLPLEDLNSYATHRGDHNTA
                     LRATLANPKLYNEMVKDENGKVKQGSLTKIMPEGKESRMWEAIETYMERKQPLIIVAG
                     TNYGQGSSRDWAAKGVRLAGVEVVIAESIERIHRTNLVGMGVLPLQFKKGDTRHTYNI
                     DGTETFDILGNIEPRGDLTVSMTRANGEKVEFKVTCRLDTTAEVEVYKAGGILQKFAK
                     DVIAE"
     gene            complement(2662..>3111)
                     /locus_tag="PKO10_01715"
     CDS             complement(2662..>3111)
                     /locus_tag="PKO10_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HNW65908.1"
                     /translation="VAFRIQERYSSADEAEKDIKERIAKKEIIIGFGHPVYATRDPRN
                     VVIKKVAKELSEENKNMLMYDVAARLEEVMWDQKKMFPNLDWFSAVSYNQMGIPTDMF
                     TPIFIIARVTGWGAHVIEQREDGKIIRPSANYTGPEPRAYVPLEDRK"
BASE COUNT          912 a          599 c          447 g         1153 t
ORIGIN      
        1 ttttcagcaa taacatcttt tgcgaatttt tgtaaaattc caccagcttt gtaaacttca
       61 acttctgcag ttgtatctaa tctacaagtt actttgaact ctactttttc accatttgct
      121 ctagtcatag aaactgttaa atcacctctt ggttcaatgt ttcctaatat atcaaatgtc
      181 tctgttccat ctatgttgta agtgtgtctt gtatcacctt ttttaaattg tagtggtaaa
      241 acacccattc caacaaggtt agttctatga attctttcaa ttgattcagc aattacaact
      301 tcaacacctg caagtcttac acctttagct gcccagtctc ttgaacttcc ttgaccatag
      361 tttgttccag caacgataat taatggttgt tttctttcca tataagtctc tattgcttcc
      421 cacattcttg actctttgcc ttctggcata atttttgtta ggcttccttg ttttacttta
      481 ccattctcat ctttaaccat ttcattgtat agttttggat ttgctaaagt tgctctaagt
      541 gctgtgttat ggtcacctct atgtgttgca taagagttta aatcctcaag tggtaatccc
      601 atttttaaac aatactcacc agatgcacta tttgcttgaa tagcatttga aggtgataag
      661 tggtctgttg taatatcatt tgggaatatt ccaagtggtc ttaagttttt aagagctggc
      721 atactcataa atgcatcttc ccaatatggt ggtttttgaa tatatgttga ttttgcatcc
      781 catttataaa atggctcagc ttttgcacct ttgtctgatt ttgcaaacat tgggtcatat
      841 atttttgcaa acatctctgg ttttacagat ttgtaaacaa ttgcatcaat ctcttcatca
      901 cttggccaaa gatctttaag tcttacttca tttccatttt tatcaattcc aataacacct
      961 gtttcaatat taactctaat acttccagca agtgcatatg caactacaag tggaggtgaa
     1021 gctaagaatg cctctttaat aaatgggtga attcttccat caaagtttct atttcctgat
     1081 aaaacagcag ttgtgtaaac accacttgct gttgcttcag cttcaatttt aggatcaagt
     1141 gctccactca ttccattaca agttgtacaa gcaaaaccta caattccaaa tcctaatttc
     1201 tctaattcac ctaaaagacc agaatctttt aaatatattt cagaaacttt tgaaccagga
     1261 gcaagtgaag attttaccca tctttttctt gtaagtccaa gctcatttgc ttttttagct
     1321 aataatccag ctgcaacaac gtttcttgga tttgatgtgt ttgtacatga tgtaatagca
     1381 gcaattaaaa ctgcaccatc tggcataaca tcatttgtaa tttcgatttt tttagcaatt
     1441 cctctatcat caagagctga tacaggaaga agtttatgtg gttgagatgg acctgctaat
     1501 gttcttgtaa cagttgttaa atcaaattta attgttcttg catatgttgc ttcacttaaa
     1561 gtatctgccc atagaccatt tgcttttgca taagtttcaa ctaattttac ttgctcagga
     1621 gatcttccag tgattcttaa ataatcaata gttctatcat caatagcaaa catagcagca
     1681 cttgcaccat actcaggagt catatttgaa atagttgctc tatcacctaa atcaagatat
     1741 ttaattccgc ttccatagaa ctctaaataa gctgaaataa cattgttatc tcttaaaaaa
     1801 gaagttaaag ataaagctat atcagtagca gttattccag cacctctagt accagtaatt
     1861 tcaactccaa tgatttctgg aaccctcata taagatggat ttccaagcat tacattttca
     1921 gcttcaagtc caccaactcc aacagcaata actccaagag catcaacatg aggtgtgtgt
     1981 gaatcagttc caacaagtgt atcaggagat gcaataccat caatattgtg aataactgga
     2041 gacattttct caaggttaat ttggtgcata ataccatttc ctggaggaat aacatctaca
     2101 ttatcaaatg cttttttagt ccagttaatg aaatcaaatc tatctgcatt tcttctatct
     2161 tcaatatctc tatttttttg gaaagcatct ggatcaaatc caccacactc aactgctaaa
     2221 gagtggtcaa cgattaattg agttggaaca actggattaa ttttttgtgg atctacacct
     2281 ttgcttgcaa ctgcttctct aagacctgct aaatcaacga aagctgtaag ccctaagata
     2341 tcatgacaga taactcttga tggataccaa gggaaatctt tgtctgttct tttttctatt
     2401 aattgaataa gtgaatcttt taaatcttca cttggacatt ttctaagtaa gttttctgct
     2461 aatactcttg atgtgtagtt aagttttgca aaagaaccag gagtgatatc ttcaactgca
     2521 ctttttacat cataatattt agtatctaaa ccatctaatt gtttaagata tttttcgttt
     2581 gtcattttat tttccgtttt ctatttttta aagtttattg taaaaaaaaa tttaggctaa
     2641 atttagccta aattttaaag attattttct atcttctaat ggaacataag ctcttggctc
     2701 tggtcctgta tagttagctg atggtctaat aatttttcca tcttctcttt gttcaattac
     2761 gtgcgcaccc catcctgtaa ctcttgcgat aataaagatt ggagtaaaca tatctgttgg
     2821 aattcccatt tggttatatg aaacagcaga gaaccagtca agatttggga acattttctt
     2881 ttgatcccac ataacttctt caagtctcgc agctacatca tacattaaca tatttttatt
     2941 ctcttcagat agctcttttg caactttttt gataacaaca tttcttggat ctcttgtagc
     3001 ataaacaggg tgtccaaatc caatgataat ctctttttta gcaattcttt ctttgatatc
     3061 tttttctgct tcatcagctg aagagtatct ttcttgaatt ctaaatgcaa c
//
LOCUS       DAOQAN010000065         3065 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_43777_length_3065_cov_3.132226, whole genome shotgun sequence.
ACCESSION   DAOQAN010000065 DAOQAN010000000
VERSION     DAOQAN010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43777_length_3065_cov_3.132226"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="PKO10_01720"
     CDS             <1..188
                     /locus_tag="PKO10_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FlgO family outer membrane protein"
                     /protein_id="HNW65909.1"
                     /translation="VVVGTYSRQNGKVILNARVIDNITGKVITSARSTYVHGLAHDCT
                     MFGDCPPMRTIKIVKEK"
     gene            193..792
                     /locus_tag="PKO10_01725"
     CDS             193..792
                     /locus_tag="PKO10_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014475443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgO family outer membrane protein"
                     /protein_id="HNW65910.1"
                     /translation="MILKKLRVFRFYLSLILLTLFLSSCGAYKDPISKNTNFDSMLND
                     MVKKSAMKIKKNVAYDEVVLVSDFVNLDNLKNRSQLGFLLSNLLKDRLSSLDVIVKEI
                     ELGKEFEFGPSGFNLLTREKNRILSDNVKSRYAVVGTYSISNKSLNLFIKLIDINSGN
                     ILSSSFARTDLDSEILDLEGISQNRDMEFRNSIRRPMVL"
     gene            complement(787..2352)
                     /gene="recJ"
                     /locus_tag="PKO10_01730"
     CDS             complement(787..2352)
                     /gene="recJ"
                     /locus_tag="PKO10_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HNW65911.1"
                     /translation="MSKITKERLFEILNARHLNNPYSKLASIPSFNNFKDINIATKRV
                     KQAILSGEKITIVGDYDVDGIVSTTIMIDFFNSLGIKVDYIIPNRFEHGYGLSVKIVD
                     MIDSGLVITVDNGITAYEASLKLKEKNIDLIITDHHTVGDKVPIALAIINPKQNDCNF
                     EFKEICGAQVAWYFCAAIKNEMNYDINMSSYLDLLCLAIIADIMPMTSLNYTMVRQGL
                     KKIKNSSREAFKLLNSHLKKDILVSDDIGFTIAPKLNSAGRMDDATVALKFLLSKDQS
                     SAYESLAILDELNSYRKTIQERISNEAEQKSNKEDRAVIVWAEDWHEGIIGIVASKLS
                     NSYKKPAFIFSIQNGIAKGSARANSNINLYDLISKASHLLLGFGGHKNAAGLSLLEEN
                     LPEFKEIINKELEKSDDILHNEFITLGELDVSSVDLEFISIIESFEPYGLANDRPFFK
                     VSNANLIKSELIGKDKNHLKLTLSSDGFVFEALKFFNSNTNLNNRLDLIISISKNEFR
                     GVVTPTFLIQEFL"
     gene            complement(2352..>3065)
                     /locus_tag="PKO10_01735"
     CDS             complement(2352..>3065)
                     /locus_tag="PKO10_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511145.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HNW65912.1"
                     /translation="SYKSLTEALIHAGAHLNTKINIHWCDSERIEDLGAYEVIGNVDA
                     ILVAGGFGARGVKGKLEAIKYARENNITFLGICLGMQLAIIEFARNVLGIEDANSIEF
                     DSETKNPLIYLIDEFIDQSGNKQLRTSKSPLGGTMRLGEYPFEPLKGSKLQKAYGNNK
                     EYFERHRHRYEANPKYKEALEKAGMIICGESNGLIEAVELQDHPWFVGVQFHPEFTSH
                     LETPNPIILDFVRKANSPK"
BASE COUNT         1067 a          463 c          389 g         1146 t
ORIGIN      
        1 atgttgttgt tggtacttac tcaagacaaa atggaaaagt tattttaaat gcgagagtta
       61 ttgataatat aactggaaaa gtaataacaa gtgcaagatc tacttatgtt catggattag
      121 ctcatgattg tacaatgttt ggagattgtc cccctatgag aactataaaa attgtaaaag
      181 agaagtagtt ttatgatttt aaaaaaatta agagtattta gattttatct atctttaata
      241 ctcttaacac tttttttaag ctcttgtgga gcatataaag acccaatttc aaaaaataca
      301 aattttgatt caatgttaaa cgatatggta aaaaaatcag ctatgaagat aaagaaaaat
      361 gtagcttatg atgaggttgt tttggtatca gattttgtaa atcttgataa tttaaaaaat
      421 agatctcaac ttggtttttt actatcaaat cttttaaaag atagattatc atctttagat
      481 gttattgtaa aagagataga actaggaaaa gagtttgaat ttggaccatc tgggtttaat
      541 cttttaacta gagaaaaaaa tagaatttta agtgataatg taaaatcaag atatgcagtt
      601 gttggaactt actctatttc taataaaagc ttaaatcttt ttataaaatt aatagatata
      661 aatagtggaa atatacttag ttcatctttt gctagaacag atttagatag tgaaatatta
      721 gatcttgagg gaatatctca aaaccgagat atggaattta gaaatagtat tagaagacct
      781 atggtcttat aaaaactctt gaattaaaaa agttggagta actactcctc taaactcatt
      841 ttttgaaatt gatattatta aatccaatct attatttaaa tttgtatttg agttaaaaaa
      901 cttcaaagct tcaaaaacaa atccatcact acttaaagtt aattttagat gatttttatc
      961 tttaccaatt agttctgatt ttataagatt tgcatttgaa actttaaaga atggtctatc
     1021 atttgcaagt ccataaggtt caaaactttc aataatagat ataaactcca aatctacact
     1081 actaacatca agttcaccta aagttataaa ctcattgtgc aatatatcat ctgatttttc
     1141 tagttcttta tttattattt ctttaaattc aggtagattt tcttccaata aagatagacc
     1201 agcagcattt ttatgtccac caaaacctaa aagaagatgt gaagcttttg atattaaatc
     1261 atacaaattt atatttgagt tcgctcttgc actacctttt gcaataccat tttggattga
     1321 aaatataaaa gcaggttttt tataactatt tgagagtttt gaagcaacaa taccaataat
     1381 tccttcatgc caatcttcag cccaaacaat aacagctcta tcctctttat ttgatttttg
     1441 ctctgcttca ttagaaattc tctcttgaat agtttttctg taactattta attcatctaa
     1501 aatagctaaa ctttcatatg cgctagattg gtcttttgat aataaaaatt ttaatgcaac
     1561 agttgcatca tccattctac cagcactatt tagttttgga gcaattgtaa atccaatatc
     1621 atcagaaact aaaatatctt tttttaagtg agaatttaaa agtttaaaag cttctctaga
     1681 agagtttttt atctttttta atccttgtct taccatagtg taattaaggc ttgtcatagg
     1741 cataatatct gcaattattg ctaagcataa aaggtctaaa tatgaactca tatttatatc
     1801 atagttcatc tcatttttta ttgcagcaca aaaataccaa gcaacttgag ctccacaaat
     1861 ctctttaaat tcaaaattac aatcattttg ttttgggtta ataattgcaa gtgctattgg
     1921 aactttatct ccaactgtat ggtggtctgt gattattaaa tcaatattct tctcttttag
     1981 ttttaaagat gcttcatatg cagttattcc attatcaaca gtaattacta aaccactatc
     2041 aatcatatca acaattttaa cagataaacc ataaccatgc tcaaatctat ttggaataat
     2101 ataatcaact tttataccca aagagttaaa aaaatctatc ataatagtag tagatacaat
     2161 accatctaca tcataatctc caacaatagt tattttttca ccactcaaaa tagcttgttt
     2221 tactcttttt gtagcaatat tgatatcttt gaagttatta aaagatggta tagatgcaag
     2281 ttttgaatat ggattattca aatgccttgc atttaagatt tcaaaaagtc tctcctttgt
     2341 gattttcgac attatttagg agagttagct tttcttacaa aatccaaaat tataggattt
     2401 ggtgtctcta aatgagatgt aaattcagga tgaaattgaa cccctacaaa ccaaggatgg
     2461 tcttgtagtt caactgcttc aataagtcca tttgattcac cgcaaataat cattccagct
     2521 ttttctaaag cctctttata ttttggattt gcttcatatc tatgtctatg tctttcaaaa
     2581 tactctttat tatttccata tgctttttgt agttttgaac cttttagtgg ttcaaaagga
     2641 tactctccta atctcatagt tccaccaaga ggtgatttac tagttcttag ttgtttattt
     2701 ccactttggt caataaattc atcaattaaa tatattagag gattttttgt ttcactatca
     2761 aattcaatag agtttgcatc ttcaattcct aaaacatttc tagcaaactc aataattgca
     2821 agttgcattc ctagacaaat tcctaagaaa gttatattat tttctcttgc atatttaata
     2881 gcttcaagtt ttcctttaac acctcttgct ccaaaaccac ctgcaactaa aatagcatct
     2941 acattcccaa taacttcata agctcctaaa tcttcaattc tttcactatc acaccagtga
     3001 atatttattt ttgtatttag gtgagcacct gcatgaatta aagcttcagt taaagattta
     3061 taaga
//
LOCUS       DAOQAN010000066         3018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_44792_length_3018_cov_3.224772, whole genome shotgun sequence.
ACCESSION   DAOQAN010000066 DAOQAN010000000
VERSION     DAOQAN010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44792_length_3018_cov_3.224772"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(38..1105)
                     /locus_tag="PKO10_01740"
     CDS             complement(38..1105)
                     /locus_tag="PKO10_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HNW65913.1"
                     /translation="MKLEKIEALTPEYLESIGFTWHTDSDDSSYICDKLVVISEDEAN
                     AYYEATNELYDMVTETAQFVIDNELFHELNIPFNLVELVKESWENDVHWHLYSRFDLA
                     GGVNGKPIKLIEFNADTPTSVFETAIIQWAMLKQNGLDEAAQFNNLYDALKDNFKRII
                     TLNSDIEKFDEYYSQLGWKILFSSISTSSEDINTTKLLEHIAQEAGFNTDFEFIENVS
                     FSDDGIFKNDELFEFWFKLIPWENIGIEESDLALLLTEIIKEKKAIIFNPAYTLIFQS
                     KAFMKILWDLYPNHPLLLGTSFEPLKGKKYVEKRAFGREGANVKIINPDGSTDIETNG
                     DYEGHKPIYQEYVEFPKDENG"
     gene            complement(1107..1742)
                     /locus_tag="PKO10_01745"
     CDS             complement(1107..1742)
                     /locus_tag="PKO10_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014474120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0323 family lipoprotein"
                     /protein_id="HNW65914.1"
                     /translation="MQNKNIKTLKELAKKGGLATFLILGLNACNDNSNQNNQSNGTFT
                     NASQQEGAFVIVEKALDGSYKIADEFPAAKTTIVLRNPDGTERILSQEEIDKLVKEEE
                     AKIDAGTSPLTNPEMSSGGMGLGGVLLSSIAGAMIGSWLGNKLFNNQNFQNQKAAQYK
                     SPQTYSKSQSSFNKPSSTAGATKQSGFFGGNNQNSSQNNNSTKSTTQSTGG"
     gene            complement(1745..>3018)
                     /locus_tag="PKO10_01750"
     CDS             complement(1745..>3018)
                     /locus_tag="PKO10_01750"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNW65915.1"
                     /translation="NENILKAIDDLGYTNPTAIQEKAIPVALGKRDIIGVAKSGTGKS
                     CAFLLPILEDLIKEKNSEKTNEKKQNSVLRALILVPTRELAKQIANAVDDYSKYIDIK
                     KVAIFGGISIKDQEKKLNAGVDIAVATTGRLLEHIKNNTINLSSVQRVVIDELDTMLD
                     MGFLEEVEQILPHIGKNRQISMFSATINSTVKKLAKEFLTDPVVIEVTNQRDHVEKIE
                     HQIVLVDEDKKSELLSYLIGSRNISQVLVFVNRKLEADDLVENLNLDGLKASCIHGDI
                     RQSSRALALRKFKEKEIRVLVCTDIAARGIDIENLPCVINFALPETINDFTHRVGRTA
                     RAGNDGVAITLLSVKDYKLMAEIEKELILKIPRVELEGFATKEKKPRTKIREKKSIKD
                     KKFDSKQRESKDKTKNKKPNKSKFRKTTKRG"
BASE COUNT          882 a          523 c          362 g         1251 t
ORIGIN      
        1 aagcttcata cgagtagaaa actccagctt gataatatta accattttca tcttttggaa
       61 actctacgta ctcttgataa attggtttat gaccttcata atcaccatta gtttctatat
      121 cagttgaacc atctggattt ataattttta catttgcacc ctctcttcca aaggctcttt
      181 tttctacata ttttttacct tttagtggct caaaagatgt tccaagcaga agtggatgat
      241 ttggatataa atcccataaa attttcataa atgcttttga ttggaagatt aatgtataag
      301 caggattaaa tataatcgct tttttctctt ttattatttc agttaaaagt aaagctaaat
      361 cactctcttc aattccaata ttttcccaag gaattagttt aaaccaaaac tcaaaaagct
      421 catcattttt gaaaatacca tcatcgctga atgatacatt ttcaatgaat tcaaaatcag
      481 tattaaatcc agcttcttgt gcaatatgtt ctaaaagttt tgtagtattt atatcttcac
      541 ttgaagttga aatactagaa aatagtattt tccaaccaag ttgtgagtaa tattcatcaa
      601 atttttctat atcactattt agcgttatta ttcttttgaa attatctttt aaagcatcat
      661 aaagattatt aaactgagct gcttcatcta aaccattttg ttttagcatt gcccactgga
      721 taattgcagt ttcaaaaact gaagtaggtg tatctgcatt aaattcaatt aatttaattg
      781 gtttaccgtt cacaccacca gctaaatcaa atcttgaata taaatgccaa tgaacatcat
      841 tttcccaaga ctcttttaca agttctacta ggttaaaagg tatatttagt tcatgaaaga
      901 gttcattatc tattacaaat tgagcagttt cagtcaccat atcataaagt tcatttgttg
      961 cctcataata agcatttgct tcatcttctg atattacgac tagtttatca caaatatatg
     1021 aactatcatc actatctgtg tgccaagtaa atccaatact ttctaaatat tcaggtgtta
     1081 gtgcttctat tttttctaat ttcatatcaa cctcctgtac tttgagttgt agattttgta
     1141 ctattattgt tttggcttga attttgatta ttaccaccaa aaaaaccact ttgttttgta
     1201 gctccagctg tacttgatgg tttattaaat gagctttgag atttactata agtttgtgga
     1261 gatttgtatt gagctgcttt ttggttttgg aaattttgat tattaaatag tttgtttcca
     1321 agccaagaac ctatcattgc tcctgctatt gaagataata atactccacc aagccccatt
     1381 cctccactac tcatttctgg gtttgtaaga ggtgatgttc ccgcatctat ttttgcctct
     1441 tcttctttaa caagtttatc aatctcttct tgtgacaaga ttctttcagt tccatctgga
     1501 tttctaagaa ctattgtagt ttttgcagct ggaaactcat ctgcaatttt atagcttcca
     1561 tcaagagctt tttcaactat aacaaaagca ccttcttgtt ggcttgcatt tgtaaaagtt
     1621 ccatttgact gattattttg gtttgagtta tcattgcaag catttaatcc taaaatcaaa
     1681 aatgtagcaa gaccaccttt ttttgccaac tcttttaatg ttttgatatt cttattttgc
     1741 atatttatcc tctctttgtt gtttttctaa atttactctt atttggtttt ttattttttg
     1801 ttttatcttt actttctctt tgttttgaat caaatttttt atcttttata gatttttttt
     1861 ctcttatttt tgttcttggt tttttctctt ttgtagcgaa cccttcaagc tcaactctag
     1921 gaattttaag tattaactct ttttctattt cagccataag tttataatct tttacactta
     1981 aaagtgttat agcaactcca tcatttccag ctcttgcagt tcttccaact ctatgagtaa
     2041 aatcatttat tgtttcaggt agagcaaaat ttattacaca aggaagattt tctatatcaa
     2101 ttccacgtgc tgcaatatct gtacaaacta aaactctaat ctctttttct ttaaattttc
     2161 tcaaagccaa tgctcttgaa ctttgtctta tatctccgtg gatacaagat gcttttaaac
     2221 catcaagatt tagattttct actaaatcat cagcttctaa ttttctattt acaaaaacta
     2281 atacttgaga tatatttctt gaacctatta aatacgataa aagttcactt tttttatctt
     2341 catctacaag aactatttga tgttctattt tttcaacatg atctctttga tttgtcactt
     2401 caattacaac aggatctgtt aaaaactctt ttgcaagttt ttttactgtt gaatttattg
     2461 ttgcactaaa cattgaaatt tgtctatttt ttccaatatg tggcaaaatt tgttcaactt
     2521 cttctagaaa tcccatatca agcattgtat caagctcatc aattacaact ctttgaacac
     2581 ttgaaagatt tatagtgttg ttttttatat gttcaagtaa tcttcctgtt gtagcaacag
     2641 caatatcaac tccagcattt aacttttttt cttggtcttt tattgaaatt ccaccaaaaa
     2701 tagcaacttt tttaatatca atatattttg aataatcatc aactgcattt gctatttgtt
     2761 ttgctaactc tcttgttgga actaatatta aagctcttaa aacactattt tgtttttttt
     2821 catttgtttt ttcgctattt ttttctttta ttaaatcttc taaaatagga agtaaaaatg
     2881 cacaactttt tcctgttcca ctttttgcaa ctcctataat atctcttttt cctaaggcaa
     2941 caggaatcgc tttttcttga atagcagttg ggttagtata tcccaaatca tctatggctt
     3001 ttaaaatgtt ttcattta
//
LOCUS       DAOQAN010000067         3012 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_44935_length_3012_cov_3.006087, whole genome shotgun sequence.
ACCESSION   DAOQAN010000067 DAOQAN010000000
VERSION     DAOQAN010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44935_length_3012_cov_3.006087"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1338)
                     /locus_tag="PKO10_01755"
     CDS             complement(<1..1338)
                     /locus_tag="PKO10_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012011989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNW65916.1"
                     /translation="MINWDEIIDKLDYAFQPIIYAQSGKLYAVEALLRNVQDIPNITT
                     IDDLFDLVFSNDYLYEFDLLLREKAIKKFANINIPNLKLFYNLDNRIIYNKNYSSGNT
                     QKILTKHNLSKDKIIFELSEKGTSIEQNALSTMLQRYKQSGYSIAIDDFGIGVSGLKL
                     LYFSEANIIKIDRFFISNIDQDSKKKLFCSSIIDMAHIMGMQVIAEGVETQKEFYTCK
                     DIGADFIQGFLVQKPTKNINEILPIYNDISNLILDDKREDQNKFIDEQFIDKIEPLPV
                     NSSLYDIILHFKKNTEHNFVPIVDEFKYFLGIIYESDIKKISYSQYGLSLAQNQNFST
                     TLLKYLKPALSVEISWGIDKILEMYNLNFQNSLGIFITHCDKYLGFINLNSLLTMSYK
                     RNIEIATNQNPLTKLPGNKQIERFIGNSFRNIQINTTHIIYFDFNDFKPFNDIY"
     gene            complement(1406..2461)
                     /locus_tag="PKO10_01760"
     CDS             complement(1406..2461)
                     /locus_tag="PKO10_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014467965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNW65917.1"
                     /translation="MSFKKTSMALIASTLLVTTLSARDQIKIVGSSTVYPFSSSVAEE
                     FGATTSFPTPVVESTGTGGGIKLFCAGVDINTPDIANASRQMKASEFKMCQENGVTDI
                     TEALIGFDGIAIAQSSKVQGFNVTKEQLALAVAAQVPSADGKSLIANPYKKWSDISNN
                     LPAREIIVYGPPKSSGTRDSFEELVLQSVFEKKAVYTDLFKKDEVANKKYKAYSEIRT
                     DGVYVESGENDNLIVQKLTKNEAAVGIFGYSFLAENKDKVVGLSIDNVFPTVETIANS
                     SYPVARSMYFYIKNQHSKDVPALNQFNKLFVSEKMIGKDGVLTELGLIPLTDDVRTKA
                     RAKVLNNEKLKAEDLKH"
     gene            complement(2524..>3012)
                     /locus_tag="PKO10_01765"
     CDS             complement(2524..>3012)
                     /locus_tag="PKO10_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnA domain-containing protein"
                     /protein_id="HNW65918.1"
                     /translation="DLDIFEVSPSDGTAEQSALLCPKCKSQFEQNCELDPNHWYCLSE
                     AMWSETPAVKVLSYRMLKKLDNQELLDMIYLDDETLSWANKGLEEFNTSVVQKDCNGV
                     VLNAGDTVTIIKDLEVKGAGFTAKRGTAVRNISLGSEEGQIEGRVNGVKIVILTQFVK
                     KS"
BASE COUNT          970 a          459 c          382 g         1201 t
ORIGIN      
        1 ataaatatca ttgaaaggtt tgaaatcatt aaaatcaaag tagattatat gggttgtatt
       61 tatttgaata tttctaaaag agtttccaat aaatctctct atttgcttat ttcctggtag
      121 ttttgttagt ggattttgat ttgttgctat ttcaatattt cttttataag acattgttaa
      181 aagagagttt aaatttataa atcccaaata tttatcgcaa tgagttataa aaataccaag
      241 agagttttga aaatttaggt tatacatctc taaaattttg tcaattcccc aagatatttc
      301 aacacttaaa gcaggtttta aatattttaa aagtgttgtt gaaaaatttt gattttgtgc
      361 taaagataat ccatattgag aataagatat tttttttatg tcgctttcgt atataattcc
      421 aagaaaatat ttgaactcat caactattgg aacaaagtta tgctcagtat tttttttgaa
      481 atgtaaaatt atgtcatata aagagctatt tacaggtaat ggttcaattt tatctataaa
      541 ttgttcatct ataaatttat tttgatcttc tcttttatca tctaaaataa gatttgaaat
      601 atcattgtaa attggtagta tttcattaat attttttgtt ggtttttgaa ctaaaaatcc
      661 ttgtataaaa tcagcaccaa tatctttaca agtataaaat tccttttgtg tttctacacc
      721 ctcagcaatt acttgcattc ccataatatg tgccatatca attatagaag aacaaaaaag
      781 ttttttcttt gagtcttgat ctatatttga tataaaaaac ctgtcaattt ttataatatt
      841 tgcttcacta aaataaagta gttttagtcc agaaactcct attccaaaat catctattgc
      901 aatagagtaa ccactttgtt tatatctttg aagcatagtt gaaagagcat tttgctctat
      961 tgaagtacct ttttcactta attcgaaaat tattttatct ttgcttaaat tgtgttttgt
     1021 aagaatcttt tgcgtatttc cagaactata atttttgttg tagattattc tattgtcaag
     1081 attataaaat agttttagat ttggaatatt tatatttgca aattttttaa tagctttttc
     1141 tcttaaaagt aaatcaaatt catataaata gtcattacta aaaactaaat caaatagatc
     1201 atcaatagtt gtaatatttg gtatatcttg aacatttcgt aataatgctt caacagcata
     1261 tagttttcca ctttgtgcgt agattattgg ttgaaaagca taatctaatt tatctattat
     1321 ttcatcccaa tttatcataa gtgtatccta aagttatttt gaaaagaggt aataaaaaga
     1381 gctttaagct ccttttattt tgttgttagt gttttaaatc ttctgctttt aatttttcgt
     1441 tatttaaaac ttttgctcta gcttttgttc ttacatcatc agttaatgga ataagtccta
     1501 actctgttaa aactccatct tttccaatca tcttttcaga tacgaaaagt ttattaaatt
     1561 gatttaaagc tggaacatct ttagagtgtt gatttttgat ataaaaatac atagatcttg
     1621 caactggata tgagctattt gcaatagttt caactgttgg aaaaacatta tcaatactta
     1681 agccaacaac tttatctttg ttttcagcta aaaatgagta tccaaaaata ccaacagcag
     1741 cttcattttt tgtaagcttt tgtactatta aattatcatt ttcaccactt tcaacataaa
     1801 caccatctgt tctaatttca gagtatgctt tatatttttt atttgcaact tcatcttttt
     1861 taaataaatc tgtataaact gcttttttct caaacacaga ttgaagtact aactcttcaa
     1921 aagagtctct agtacctgat gattttggtg gaccataaac tataatttct ctagcaggca
     1981 aattattact aatatctgac cattttttgt aagggtttgc aataagtgat tttccatcag
     2041 ctgatggaac ctgtgcagca acagctagtg ctaattgctc ttttgttaca ttaaaacctt
     2101 gtacttttga actttgtgca atagcgattc cgtcaaaacc gattaatgct tcagttatat
     2161 ctgtaacacc attttcttga cacattttaa attcgcttgc tttcatttgt cttgaagcat
     2221 ttgcaatatc tggagtattt atatccactc cagcacaaaa tagttttatt cctccacctg
     2281 ttcctgttga ttcaacaact ggtgttggaa aagatgttgt agcaccaaac tcttcagcaa
     2341 ctgaagatga aaaaggataa actgttgatg aaccaacaat tttgatttga tctcttgcac
     2401 ttaaagttgt aactaaaagt gtacttgcta ttaaagccat agatgttttt ttgaaactca
     2461 ttttacttcc taataatttg atatttggaa gtttagtttc gcttggttac aatttggtta
     2521 cctttaagat ttttttacaa attgagttag aataactatt ttaacaccat ttacacgacc
     2581 ttctatctga ccttcttcac ttcctaatga gatatttctt acagcagttc ctctttttgc
     2641 agtaaatcca gcacctttta cttcaaggtc tttgattata gtaactgtat ctccagcatt
     2701 taaaacaaca ccattacaat ctttttgtac aacactagtg ttaaactctt ctaaaccttt
     2761 atttgcccaa gataaagttt catcatctaa ataaatcata tccaaaagct cttgattgtc
     2821 tagttttttt aacattctgt atgataaaac ttttactgca ggagtttcgc tccacatagc
     2881 ttcacttaga caataccaat ggtttggatc aagttcacaa ttttgttcaa attgtgattt
     2941 acattttgga cataaaagtg cagattgttc tgctgttcca tcacttggtg atacttcaaa
     3001 aatatctaaa tc
//
LOCUS       DAOQAN010000068         3005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_45099_length_3005_cov_3.438644, whole genome shotgun sequence.
ACCESSION   DAOQAN010000068 DAOQAN010000000
VERSION     DAOQAN010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45099_length_3005_cov_3.438644"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            205..693
                     /gene="rplJ"
                     /locus_tag="PKO10_01770"
     CDS             205..693
                     /gene="rplJ"
                     /locus_tag="PKO10_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014475175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HNW65919.1"
                     /translation="MTRQEKSEIIDFLAAEFKNSLAVVVCDYKGLTHKELETLRKDAR
                     ANNTKVQVVKNTLVTKAVKSADLGDIDLSGTNIYLWSDDQISACKVADKFATTTKDKF
                     SIKSGIIEGEICDATRVNAFAKLPSREELLGMLASVWMGPVRNFTIGLDALRRKKEEE
                     AA"
     gene            741..1109
                     /gene="rplL"
                     /locus_tag="PKO10_01775"
     CDS             741..1109
                     /gene="rplL"
                     /locus_tag="PKO10_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002858569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HNW65920.1"
                     /translation="MAISKEDVLEFISGLSVLELSELVKEFEEKFGVSAQPVAVAGAA
                     VAVEAAEEKTEFDVIIVDSGDKKINVIKEIRAITGLGLKEAKDAAEQTPSTIKEGISK
                     ADAEAIKAQLEAAGAKVEVK"
     gene            1276..>3005
                     /locus_tag="PKO10_01780"
     CDS             1276..>3005
                     /locus_tag="PKO10_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HNW65921.1"
                     /translation="MLNSLKSGNRLRIDFAKNPQKIEIPNLLQLQQTSYDTFLMIDQK
                     DRSGAGIEKVFKAIFPIHDAQNRVTLDYLGSEVGKPRYDVRESMVRGLTYSIPLKINV
                     RLTLWDLDEKTGEKIGVKDIKEQSLFIREIPLMTERTSFIVNGVERVVVNQLHRSPGV
                     IFKEDESNTTNNKLLYTGQIIPDRGSWLYFEYDAKDVLYVRINKRRKVPITILFRALG
                     YSKEDIVKLFYPVITIKIKNNKFLTEFNPDDFMGRIDYDIKDDKGNLVIQAGKRLTAR
                     KAKALIEGGLKLIEYPLELLMDRHTAGSIYDPQSGEVLFDALTSLDELKLKKLLDLGF
                     ETFEIANDLSSGADASIINAFKADAESLKLLKQTEQIEDENDLAAIRIYKVMRPGEPV
                     TKEAAKEFIKKLFFDPERYDLTKVGRMKMNHKLGVNVPEYVTTLTYEDVIKTVQYLIK
                     VKAGHGHIDDRDHLGNRRIRAIGELLANELHAGLIKMQKTIRDKMTTLSGTLEDIMPH
                     DLINSKMITSTITEFFTSGQLSQFMDQTNPLSEVTHKRRLSALGEGGLVKERAGFEVR
                     DVHPTHYGRI"
BASE COUNT         1174 a          350 c          538 g          943 t
ORIGIN      
        1 atcagttatt ttagataata tggaattatt agagattaga taagggtttc cttatctaat
       61 ttttttgcat tcaagaatat tgaaattgtt cactattttt gagtgttcaa agcactgaaa
      121 taaaactgaa gacagctggt aaaagaactc ctcgttcttt tgtaagtccc gcctaggatg
      181 atgttttgaa aggaggaaaa tataatgact agacaagaaa aatcagaaat tattgatttt
      241 ttagcagctg agtttaaaaa ctctttagca gttgttgttt gtgactataa aggtcttact
      301 cataaagaat tagaaacttt aagaaaagat gcaagagcaa acaatactaa agttcaagtt
      361 gtaaaaaata cattagtaac taaagctgtt aaatctgctg atttaggaga tattgattta
      421 agtggaacaa acatctactt atggtctgat gatcaaattt cagcttgtaa agtagctgat
      481 aaatttgcaa ctactacaaa agataaattc tctattaaat caggaattat tgaaggtgaa
      541 atttgcgatg ctactagagt taatgcattt gctaaattac catcaagaga agaacttctt
      601 ggaatgcttg catctgtatg gatgggacct gttagaaact ttactatcgg tcttgatgct
      661 cttagaagaa aaaaagaaga agaggctgct taattttaag ctaaaaatat attaaattaa
      721 aaaaataaaa aggatagaaa atggcaattt ctaaagaaga cgttttagaa tttatttctg
      781 gtttatctgt attagaatta tcagaattag taaaagaatt tgaagaaaaa tttggagtat
      841 ctgcacaacc tgtagcagtt gctggtgctg cagttgcagt tgaagctgct gaagaaaaaa
      901 ctgaatttga cgtaattatt gttgattcag gagataaaaa aatcaatgta attaaagaaa
      961 ttagagcaat tactggttta ggattaaaag aagcaaaaga tgctgcagag caaactcctt
     1021 caacaattaa agagggaatt tctaaagctg atgctgaagc aatcaaagct caacttgaag
     1081 ctgctggtgc aaaagtagaa gttaaataag ttttataact tatcgtatat agggactttt
     1141 ccctatataa gtttagcatc tttgagggct aaggttaaga ttttttaaca aattttaacc
     1201 ttatccttta tggatgcttt tgtgctttat aagaaaataa agcaaacata accaaatttt
     1261 ataacaaggt gtaagatgtt aaactcttta aaatctggta atagactaag aatagatttc
     1321 gcaaaaaacc cacaaaaaat tgaaattcca aatttattac aactgcaaca aacttcgtat
     1381 gatactttct tgatgattga tcaaaaagat agatctggtg ctggtataga aaaagtattt
     1441 aaagcaattt ttccaattca tgatgctcaa aatagagtaa ctcttgatta tttaggaagt
     1501 gaagttggaa aacctagata tgatgttaga gaatcaatgg ttagaggatt gacatattca
     1561 atccctctta agataaatgt aagattaact ctttgggatt tagatgagaa aactggagag
     1621 aaaataggtg taaaagatat taaagagcaa tctttattta ttagagaaat cccacttatg
     1681 actgaaagaa cttcatttat tgtaaatggt gttgaaagag ttgttgtaaa tcagttacat
     1741 agatctccag gtgttatttt caaagaagat gaatcaaata caacaaataa taaacttctt
     1801 tacactggac aaattatccc agatagaggt tcttggttat attttgaata tgatgcaaaa
     1861 gatgtattat atgtaagaat taataaaaga agaaaagttc caattacaat tctttttaga
     1921 gctttaggat attcaaaaga ggatattgtt aaactatttt atcctgtaat tactattaaa
     1981 attaaaaaca ataaattttt aacagagttt aatcctgatg actttatggg aagaattgac
     2041 tatgatataa aagatgataa aggaaattta gttattcaag caggaaaaag attaactgca
     2101 agaaaagcta aagctttaat tgaaggtgga ttaaaattaa ttgaatatcc acttgagctt
     2161 ttaatggata gacatacagc aggttctatt tatgatcctc aatcaggaga agttttattt
     2221 gatgctttaa caagtcttga tgagttaaaa cttaaaaaac tattagattt aggatttgaa
     2281 acatttgaaa ttgcaaatga tttatcaagt ggagctgatg cttcaattat aaatgcattt
     2341 aaagcagatg ctgaaagttt aaaattatta aagcaaactg aacaaattga agatgaaaat
     2401 gatttagctg caattagaat ttataaagta atgagaccag gtgagcctgt aacaaaagag
     2461 gcagcaaaag agtttataaa aaaactattc tttgatcctg aaagatacga cttaacaaaa
     2521 gttggaagaa tgaagatgaa tcataagtta ggtgtaaatg tacctgaata tgttacaact
     2581 ttaacttacg aagatgttat aaaaactgtt caatacttaa taaaagttaa agcagggcat
     2641 ggtcatattg atgatagaga tcacttagga aatagaagaa ttagagcaat tggggaatta
     2701 cttgcaaatg agttacatgc aggtttaatc aaaatgcaaa aaactattag agataaaatg
     2761 acaactctaa gtggaacttt agaagatatt atgcctcatg atttaattaa ctcaaaaatg
     2821 attacatcaa caattactga gttttttaca agtggtcaat tatcacaatt tatggatcaa
     2881 acaaatccac tatctgaagt tactcataaa agaagattat cagctcttgg agagggtggt
     2941 ttagtaaaag agagagcagg atttgaggta agggacgttc atccaactca ctacggaaga
     3001 atttg
//
LOCUS       DAOQAN010000069         3001 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_45188_length_3001_cov_3.211813, whole genome shotgun sequence.
ACCESSION   DAOQAN010000069 DAOQAN010000000
VERSION     DAOQAN010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45188_length_3001_cov_3.211813"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..446
                     /locus_tag="PKO10_01785"
     CDS             <1..446
                     /locus_tag="PKO10_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNW65922.1"
                     /translation="LIFTLGFKEAIIVSLTVPMILSLTLFIGFMLGESVNRITLFALI
                     VALGMLVDAAIIVIENIHRHKIEHPNMDLETLAINATNEIGNPTNIATIAIIMVFIPM
                     FFVGGMMGEFMHPLPVFVPISLAVSLFVAYAFTPYLVKKILKGKK"
     gene            443..1549
                     /locus_tag="PKO10_01790"
     CDS             443..1549
                     /locus_tag="PKO10_01790"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNW65923.1"
                     /translation="MRLEKFILDILNSKRKINLIYLITLILFILSILTFPTKIVKAKM
                     LPNKDSDSFSIYLDLKDGSSKYETKEVVDCIVKNLQDEENITNISAFISQGQPIDFAG
                     LVKQSALKNSENQAELMINIKRFKDREITSYNLISNLRTKIQDSCQKKYEATIKFIEL
                     PAGPPVLASLVAEIYGGDSFSSRRDFAIKVANIFKNQDTLVDIDILASKDFFTYTLEI
                     NSNKAIMSQVDLEHLKATLYLAFEGMSLGVINDKNAQSQIPIFLRLDDSRNLSNNSKD
                     ALNLKLNSLKIMNNIGQMISVSELVNIKQTIKEPTITSKNLNPIINVIAETSNDSQIY
                     PLLSSREDMIKSFSNDYEIIKTNMLNLSFIDKKR"
     gene            complement(1594..2796)
                     /locus_tag="PKO10_01795"
     CDS             complement(1594..2796)
                     /locus_tag="PKO10_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014768976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HNW65924.1"
                     /translation="MKIEIDVEQFANDLKAGKSIGGANGALGSLIKQLTEAALAAEID
                     SHLAKDLSNNRRNGYSSKTMKSDHGTFELDVPRDRNGNFEPEIVKKNQTSMTSEIEDK
                     ILSLFALGNSYSQIAKYIEDFYCVGFSKATISAVTDKIIPMLNEWKTRPLETVYPFVF
                     LDAIHYKVKEDGKYIAKAFYTVLGVKVDGKKEVLGLYLNESEGAKFWLQVLTDLQNRG
                     VKDILIASVDGLKGFPEAINSVFPNTQVQLCIVHQIRNSIRYIGSLNQKQFAQELKAV
                     YQAFTKEEALYELDKLEEKWGKKYPIVFQSWRNKWENLTVYFQYPEDIRRVIYTTNII
                     ESVHRQFRTLTKTKGAFPNDDSLLKLLFMGIKNAEQKWTMPIRNWSLTLSQLAIHFEG
                     RLDDSLNL"
BASE COUNT         1041 a          435 c          393 g         1132 t
ORIGIN      
        1 tacttatttt tactttgggg tttaaagagg cgattattgt atctttaact gttcctatga
       61 ttttatcttt gactttgttt attggattta tgttaggcga gagtgtaaat cgtataacac
      121 tttttgcact tattgttgct ttaggaatgc tagttgatgc tgcaataatt gtaatagaaa
      181 atatacatag acataaaatt gaacatccaa atatggattt agaaactcta gcaataaatg
      241 caacaaatga gataggaaac cctacaaata tagcaactat tgcaataatt atggtattta
      301 ttcctatgtt ttttgtaggt ggaatgatgg gagaatttat gcaccctctt cctgtttttg
      361 ttcctatatc tttggctgtt tcactttttg ttgcatatgc ttttacacct tatttagtta
      421 aaaagatttt aaaaggtaaa aaatgagatt agagaaattt atactagaca ttttaaatag
      481 taaaagaaaa ataaatttaa tttatcttat tactttaata ctttttatct taagtatttt
      541 aacttttcca acaaaaatag taaaagcaaa aatgcttcca aacaaagatt cagatagttt
      601 ttctatatat ttagatttaa aagatggttc aagtaaatat gagacaaaag aggttgtaga
      661 ttgtattgta aaaaatctac aagatgagga gaatataaca aatatttcag cttttatatc
      721 tcaaggtcaa ccaattgatt ttgcaggact tgtaaaacaa agtgctttaa aaaatagtga
      781 aaaccaagct gaacttatga taaatattaa aagatttaaa gatagagaaa ttacaagtta
      841 taatcttatt tcaaatctta gaactaaaat tcaagactct tgtcaaaaaa aatatgaagc
      901 aactataaaa tttatagaac ttcctgctgg tcctccagta cttgcttctt tggtagctga
      961 aatttatggt ggagatagtt tttctagtag aagagatttt gctataaaag tagcaaatat
     1021 ttttaaaaat caagacactt tagttgatat tgatatttta gcttcaaaag atttttttac
     1081 ttacacttta gaaataaata gcaacaaagc tattatgagc caagttgatt tagaacattt
     1141 aaaagccact ttatatcttg catttgaagg tatgtcatta ggagttataa atgataaaaa
     1201 tgcccaaagc caaattccaa tatttttaag acttgatgac tcaagaaatt tatcaaataa
     1261 ctcaaaagat gctttaaatc ttaaacttaa tagcttaaaa ataatgaata atataggtca
     1321 aatgattagt gtttcagagt tagtaaatat aaaacaaact ataaaagagc caacaataac
     1381 atcaaaaaat ctaaatccaa taataaatgt aatagctgaa acttcaaatg atagtcaaat
     1441 atatccactt ttaagttcaa gagaagatat gataaaaagc ttttcaaatg attatgaaat
     1501 aatcaaaaca aatatgctaa atctatcttt tatagataaa aaaaggtagt gttcaatatt
     1561 ctgtgtcatc atcagataag tcctaaaatt ctatcataaa tttaaactat catcaagccg
     1621 tccttcaaag tggatggcta gttgagacaa agttaaactc caatttctaa ttggcattgt
     1681 ccatttttgt tctgcatttt ttattcccat aaacagtagt ttaagtaagc tatcatcatt
     1741 tggaaatgca cccttcgttt tagttaaagt tctaaattgt ctatgaacag attcaataat
     1801 atttgtagta taaattactc ttcttatatc ttcaggatat tggaaataaa ctgttaaatt
     1861 ttcccattta tttctccagg attgaaatac tatagggtat tttttacccc atttttcttc
     1921 aagtttatca agttcatata atgcttcttc ttttgtaaaa gcttgatata cagcttttaa
     1981 ttcttgggca aattgttttt gatttaatga tccaatatat ctaattgaat ttctaatttg
     2041 atgaactata caaagttgaa cttgagtatt tggaaataca gaattaattg cttctggaaa
     2101 gccttttaaa ccatcaactg aggcaataag aatatctttt acgccacggt tttgtaaatc
     2161 tgttaaaact tgtaaccaga actttgctcc ttcactctca tttaagtaaa gtcctaatac
     2221 ctctttttta ccatcaactt taactcctaa aactgtataa aaagcttttg ctatatattt
     2281 accatcttct tttactttat agtgtattgc atcaagaaat acaaatggat atactgtttc
     2341 taatggtctt gttttccatt catttagcat tggtattatt ttatctgtta cagcacttat
     2401 tgttgctttt gagaagccta cgcaataaaa atcctctata tattttgcta tttgtgagta
     2461 gctattacct aatgcaaaca aagataatat tttatcttct atttcacttg tcatgcttgt
     2521 ttgatttttc tttacaattt ctggttcaaa gttaccattt ctatctcttg gaacatctaa
     2581 ttcaaatgtt ccatgatcac ttttcatggt ctttgagcta taaccatttc ttctattatt
     2641 actcaaatct tttgcaagat gtgagtctat ctcagctgca agtgcagctt ctgttaattg
     2701 tttgattaaa gaacctagag caccatttgc tcctccgata ctttttcctg cttttagatc
     2761 attagcaaat tgctctacat ctatttcgat tttcatattt gtgtcctttg gtattttcta
     2821 ttttacctaa ttgacacaaa attctgaacg gtcccaacac taccgtaaaa atcatgttat
     2881 caattctaaa ctctctattt aaccaataat aaatatcatg tgctatatgt ttaatatttg
     2941 ttgcatattg taattgctca tatagcttaa atatttttac taaactatta tatttagtcc
     3001 t
//
LOCUS       DAOQAN010000070         2997 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_45296_length_2997_cov_2.369477, whole genome shotgun sequence.
ACCESSION   DAOQAN010000070 DAOQAN010000000
VERSION     DAOQAN010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2997
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45296_length_2997_cov_2.369477"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="PKO10_01800"
     CDS             complement(<1..698)
                     /locus_tag="PKO10_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HNW65925.1"
                     /translation="MNNIRLFHISHTDLDGYACQFLTNEVFSKKHFYNANYGLEVKQA
                     IKQVITEIKNYQDEKLLLLISDLNLNQQESDELDKDIKSLKDDGFDITLQLLDHHITG
                     KSSAAKYDWYFLDDKRCATKIVFDYLLKEFKAPLNDYKEWVDTVNAVDIWLENEKKNF
                     EFGKVLMSMVTKAREINSILFNSLDRDFKFYLLKNSIGFLSLKDAPIILDNSVHSLKK
                     EFLKDGFDDTLDNL"
     gene            complement(691..1095)
                     /locus_tag="PKO10_01805"
     CDS             complement(691..1095)
                     /locus_tag="PKO10_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004317794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNW65926.1"
                     /translation="MLLTKKSEYALLSLLSISKHKEPINVDILSKELQISKSFLAKIM
                     QNLAKANLVISHRGVNGGFVLNKPIEELTILEIVVAAEEKNPMVFECSDAISSCPNNK
                     AKICTIWPLLNNLQFKVNDLLAKLTLKDIEHE"
     gene            complement(1213..1485)
                     /gene="rpsO"
                     /locus_tag="PKO10_01810"
     CDS             complement(1213..1485)
                     /gene="rpsO"
                     /locus_tag="PKO10_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004317763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HNW65927.1"
                     /translation="MALDQEVKASIIAKYGRKDGDTGSAEVQIALLSEQIKNLTEHLK
                     VFKKDHSSRLGLLKMVGKRKKLLSYLKKSDYARFTSVVESLGIRAK"
     gene            complement(1585..2556)
                     /gene="moaA"
                     /locus_tag="PKO10_01815"
     CDS             complement(1585..2556)
                     /gene="moaA"
                     /locus_tag="PKO10_01815"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HNW65928.1"
                     /translation="MLIDGFGRKHDYLRVSVTERCNFRCHYCMPEKPFSWVPKENLLS
                     YEDLFKFIKIGIDEGIKKVRITGGEPLLRDNLDYFVKLISDYKNDIDLALTTNGFLLP
                     QMAQKLKDAGLKRINISLDTLNQERAMKIAQKDVLPTVLEGIYKAKDVGLKIKINCVP
                     LKNINDIDILDVLEFCKKENFSVRYIEFMENSFAKNGAKGLNSDEILEIISKKYKNII
                     KVPRDNSSPAQYYRLEDGYEFGIIEPHKDDFCASCNRIRLTAEGFLIPCLYFEDALSI
                     KDAIKNNQIDEATLILKKVLQNKPEKNKWSNKDDNKVSSRAFYETGG"
     gene            complement(2569..>2997)
                     /locus_tag="PKO10_01820"
     CDS             complement(2569..>2997)
                     /locus_tag="PKO10_01820"
                     /inference="COORDINATES: protein motif:HMM:NF012800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HNW65929.1"
                     /translation="LRNEFGLRTAFNTVEKLLNPSLSEYGVCGAFHKPYVSKYLEMFK
                     DDFKDIIVIRGNEGDIEVFKDSKFWQKKDGEIKEYDFCLKDYGVSYSKVFENITLEEN
                     LNILRNYDDEILNLAKFNVALYLLFASRVDSLDEAWQRLN"
BASE COUNT         1006 a          484 c          292 g         1215 t
ORIGIN      
        1 cttaaattat ctaaagtatc atcaaatcca tcttttagaa actctttttt caaactgtgt
       61 acactattat ctaaaattat aggagcatct ttaagagata aaaatcctat agaatttttt
      121 agtaaataaa atttaaaatc tctatcaaga gagttaaaaa gtatagagtt tatctctctt
      181 gcttttgtaa ccatcgacat taaaactttt ccaaattcaa aattcttttt ctcattttca
      241 agccaaatat ctaccgcatt tacagtatca acccactctt tataatcatt taaaggtgct
      301 ttaaactctt ttaaaagata atcaaaaacg atttttgtag cacatctttt atcatctaga
      361 aaataccaat catattttgc cgcacttgat tttccagtta tatgatgatc taaaagttgt
      421 aaagttatat caaaaccatc atcttttaaa gattttatat ctttatcaag ctcatcgctt
      481 tcttgttggt ttaaatttaa atcacttatt agaagtagta atttttcatc ttgataattt
      541 ttaatttctg ttataacttg ttttattgct tgttttactt ctaaaccata gtttgcatta
      601 taaaaatgtt ttttagaaaa tacctcattt gtcaaaaatt gacaagcgta accatctaaa
      661 tctgtgtgag aaatatgaaa aagtcttata ttattcatgt tctatatctt tcaatgttaa
      721 ttttgctaaa aggtcattta ctttgaattg tagattattt aaaagtggcc aaatagtaca
      781 aattttcgct ttattatttg gacaagaact tattgcatct gaacattcaa aaaccatagg
      841 atttttctct tcagcagcaa caacaatttc taaaattgtg agctcttcta ttggtttatt
      901 taaaacaaaa ccaccattta cacctctgtg agatattact aaatttgctt ttgctagatt
      961 ttgcataatt tttgctaaaa atgattttga aatttgtaac tcttttgata gaatatcgac
     1021 atttataggc tctttatgct ttgatataga tagcaaagat aacaatgcgt attcactctt
     1081 ttttgttaat aacatttttt atccaatata aaattttatt tatattgtac aaaagttctt
     1141 attaatttta agtttccata aaaaaaggga ttatggaaac cataatccct tttcgaagtt
     1201 taaagtctaa atttatttag ctctaattcc taagctctca actacagatg taaatcttgc
     1261 atagtctgat ttttttaagt atgataaaag tttttttctt tttcctacca ttttaagtag
     1321 tccaagtctt gaagagtgat cttttttaaa tacttttaag tgctctgtta aatttttaat
     1381 ttgctcactt aatagtgcaa tttgaacctc agctgaacct gtatctccat cttttcttcc
     1441 atattttgcg ataatacttg cttttacttc ctgatctaaa gccattttga cctcctaata
     1501 ggtatattta tttttgttct atttaaaata ggcgcgaatt ttatataaag tttgctttat
     1561 atttacttta aaattatatt tttattatcc accagtttca taaaaagctc tacttgatac
     1621 tttattatca tctttatttg accatttatt cttttctggt ttattttgaa gaactttttt
     1681 aagaatcaaa gttgcttcat caatttgatt attttttatt gcatctttta tactaagagc
     1741 atcttcaaaa tataaacaag gaattaaaaa cccttcagct gttaatctta tacgattaca
     1801 agaagcacaa aaatcatctt tatgtggttc aataattcca aactcatatc catcttctaa
     1861 tctataatat tgagctggtg agctattatc ccttggaact tttattatat ttttatattt
     1921 ttttgaaata atctctaaaa tctcatcaga gtttaatcct tttgcaccat tttttgcaaa
     1981 gctattctcc ataaattcga tataccttac agaaaaattc tcttttttgc aaaattctaa
     2041 aacatctaaa atatcaatat catttatatt ttttaaagga acacaattaa tttttatttt
     2101 aagcccaaca tcttttgctt tataaattcc ttccaaaact gtaggtaaaa catctttttg
     2161 agctattttc atagctcttt cttgatttaa tgtatcaaga gagatattta ttcttttaag
     2221 tccagcatct tttaattttt gtgccatttg aggaagtaaa aaaccatttg tcgtaagagc
     2281 caaatctata tcatttttat aatcacttat taattttaca aaataatcaa gattatctct
     2341 taaaagtggc tctccaccag taattctaac tttttttata ccttcatcaa tacctatttt
     2401 tataaattta aatagatctt cataagataa taaattctct ttaggaaccc aagaaaaagg
     2461 tttttcaggc atacaatagt gacatctaaa gttacatctt tcagttactg aaactcttag
     2521 ataatcatgt tttcttccaa atccatcaat taacatctta ttcctttttt aatttaacct
     2581 ttgccaagct tcatctaaag agtcaactct tgaagcaaaa agaagatata aagccacatt
     2641 aaatttagct aaatttaata tctcatcatc ataatttctt aaaatattta aattctcttc
     2701 caaagttata ttttcaaaaa cttttgaata actaactcca taatctttta gacaaaaatc
     2761 atactctttt atctcaccat cttttttttg ccaaaatttt gaatctttaa atacttcaat
     2821 atcaccctca tttcctctaa ttactataat atctttaaaa tcatctttaa acatctctaa
     2881 atattttgaa acataaggtt tatgaaaagc tccacaaact ccatactcgc tcaaagatgg
     2941 atttaaaagt ttttcaactg tattaaaagc tgttcttaat ccaaactcat ttcgtag
//
LOCUS       DAOQAN010000071         2977 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_45740_length_2977_cov_3.160507, whole genome shotgun sequence.
ACCESSION   DAOQAN010000071 DAOQAN010000000
VERSION     DAOQAN010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45740_length_2977_cov_3.160507"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(114..1379)
                     /locus_tag="PKO10_01825"
     CDS             complement(114..1379)
                     /locus_tag="PKO10_01825"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012013140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HNW65930.1"
                     /translation="MFIHIDLDCYFVSAHRTLDKSLHNIPVAVGGKSNVDIFSHTRVK
                     RTMATNRASFSSKILDSEDENSSNDYFVDENNKIRGIITSASYEARAFGVKTAMSVNE
                     ALKLCPNLKMIPPKYNLYDELSSKLKELLELEIPLIEQFSIDEFFGDLTGYIKEDEAE
                     DFARKLKDKIFKELNLPCSIGLANTKYLSKLMTNEAKPNNIKLLKKENIEEFTKNILI
                     ENFTGIGKSLCEKLSGYNIKTLGDIRKNKNLFYSFGKVGIDTYNRVCGIKDSKLNITK
                     EKKSIGIGRSFDVVFNREELKRRVMILSRYLSFIVKKDGHNPLSFQIHIKYESNIKTK
                     AQENSNKIFNEFDFKNSIINLFMQADKHRNHGVVQLYITVFNFAKKGEHTYNLFEYED
                     DLKKDKLGKNIQNLREKFGIDILKSACEL"
     gene            1591..1887
                     /locus_tag="PKO10_01830"
     CDS             1591..1887
                     /locus_tag="PKO10_01830"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNW65931.1"
                     /translation="MKKLAFLLATFASLSFAVDYDPVRGEMLSLSCANCHGTDGKSTT
                     AIPKIAGLDKNYMYQTLLEYKTGKRVDTHMMQKHTKGFTDEELEQLSYYFSKVK"
     gene            1904..>2977
                     /locus_tag="PKO10_01835"
     CDS             1904..>2977
                     /locus_tag="PKO10_01835"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014474688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HNW65932.1"
                     /translation="MINRRDFGKLVLGATALSFTACNSLASGLSTIPANKKRVVIVGG
                     GFGGAATARYLRKFDSNVEIVLVEQNKEYYTCPFSNAVIAGMEKMDFIKHDLSTLAKK
                     HNIKVVHAKVAKIDGANQNVVLENGEKISYTKAVVSPGIDFKYEKGYTKENEKHAPHA
                     VKAGEQTTILQKQLENMKDGGTFVMVAPADPFRCPPGPYERISLVAHYIKNNKPNSKI
                     IVLDQKDKFSKQVLFTNGWKELYGDLIEWRAAQFGGKVVSVDPVKKIVVTEDEEVQAD
                     VLNYIPNQKASQLAFDSGLVADGKDWCDIHPKTFESKLVKNVHVIGDASNAAPMPKSA
                     FSASTQGKVVALQIARMLKQQEPL"
BASE COUNT         1060 a          407 c          388 g         1122 t
ORIGIN      
        1 ctttaatatt ttttgaatca tatctatttt taatatcact tttttttaaa ttataagatt
       61 ataactaaat ctattttaaa atagttaaaa tattacaaat tgtcatattt taactatagt
      121 tcacaagcac tttttagaat atcaattcca aatttttctc ttagattttg tatattttta
      181 cccaatttat cttttttcag atcatcttca tactcaaata aattataagt atgttctcct
      241 ttttttgcaa aattaaaaac tgtaatatat aattgaacta caccatgatt tctgtgtttg
      301 tctgcttgca taaatagatt tattatagag tttttaaaat caaattcatt aaaaatctta
      361 ttactatttt cttgtgcttt tgttttaata tttgactcat attttatgtg aatttgaaaa
      421 gacaaaggat tatgcccatc tttttttact ataaaactca aatatctact caaaatcata
      481 actcttcttt taagctcttc tctattaaaa actacatcaa aacttcttcc aattcctata
      541 ctttttttct cttttgttat atttagttta ctatctttta ttccacaaac tctattatat
      601 gtatctattc caacttttcc aaaagagtaa aatagatttt tattttttct aatatctccc
      661 aaagttttta tattgtaacc acttaatttt tcacaaagag attttccaat tcctgtaaag
      721 ttttctatta aaatattttt tgtaaactct tcaatatttt cttttttcaa aagttttata
      781 ttgtttggtt ttgcctcatt tgtcataagc tttgataaat attttgtatt tgctaagcca
      841 atagagcaag gaagatttaa ctctttaaat atcttatctt ttaattttct tgcaaaatct
      901 tctgcttcat cctcttttat ataaccagtt aaatcaccaa aaaattcatc aatagagaac
      961 tgctctatta gaggaatttc aagttccaaa agctctttta attttgaaga caactcatca
     1021 taaagattat attttggtgg tatcattttc aaatttggac aaagttttaa agcttcattt
     1081 acactcatag ctgtttttac tccaaaagct cttgcctcat aagaagctga tgttataatt
     1141 cctcttattt tattattttc atcaacaaaa taatcatttg aactattttc atcttcacta
     1201 tctaaaattt tacttgaaaa actagcacga tttgttgcca ttgttctttt tactcttgta
     1261 tgtgaaaaaa tatctacatt acttttccca ccaacagcaa cgggaatatt atgtaaactt
     1321 ttatcaagag ttctatgtgc tgatacaaaa tagcaatcta agtcaatatg aataaacata
     1381 ataaaatatt actctatttt taataaaata tttaataaat attgcaattt gcaatatttt
     1441 tatcttttat gtcttaataa aaaattgaga agaatttaca cataattttt ttttataatt
     1501 caatataaga tttatttaat attttaaatt tttattaagt ttattaaatt atcattcatt
     1561 tgtatttgtt atataaaagg agaagataaa atgaaaaaac tggcattttt acttgcaact
     1621 ttcgcaagtt tatcgtttgc tgtagattat gaccctgtaa gaggagagat gttatctctt
     1681 tcttgtgcaa actgtcatgg aactgatgga aaatctacta ctgctattcc aaaaatagct
     1741 ggattggata agaattatat gtatcaaact ttacttgagt acaaaactgg aaaaagagtt
     1801 gatacacata tgatgcaaaa gcatacaaaa ggtttcactg atgaagagct tgagcaacta
     1861 tcttactact tttcaaaagt taaataagca aaaaggataa acgatgataa atagaagaga
     1921 ttttggaaaa ttagttttag gagctacggc tctatctttt actgcttgca atagtttagc
     1981 aagtggttta tctacaattc ctgcaaataa aaaaagagtt gtaattgtag gtggtggttt
     2041 tggtggagct gcaactgcta gatatttaag aaaatttgat tcaaatgttg agattgtttt
     2101 agttgaacaa aataaagagt attatacttg tccattttca aatgcagtaa ttgctggtat
     2161 ggaaaaaatg gattttataa aacatgattt atctacttta gcaaaaaaac ataatattaa
     2221 agtagtacat gcaaaagttg caaaaattga tggtgcaaat caaaatgttg ttttagaaaa
     2281 tggagaaaaa atctcttata caaaagctgt tgtaagccct ggaattgatt ttaaatatga
     2341 aaaaggttat acaaaagaga atgaaaaaca tgctccacat gctgtaaaag ctggtgagca
     2401 aactacaatt cttcaaaaac aacttgaaaa tatgaaagat ggtggaactt ttgtaatggt
     2461 agcacctgct gatcctttta gatgtcctcc tggaccttat gagagaatct cacttgtagc
     2521 tcactatatt aaaaataata aaccaaattc aaaaattata gttcttgatc aaaaagataa
     2581 attctcaaaa caagttctat ttacaaatgg ttggaaagag ctttatggtg atttaattga
     2641 gtggagagca gcacaatttg gtggaaaagt tgtaagtgtt gatcctgtta aaaaaatagt
     2701 tgtaactgaa gatgaagagg ttcaagcaga tgttttaaac tatattccaa atcaaaaagc
     2761 ttctcaatta gcatttgatt caggacttgt agcagatgga aaagattggt gtgatattca
     2821 tccaaaaact tttgaatcta aattagttaa aaatgttcat gttattggag atgcttcaaa
     2881 tgcagcacct atgcctaaat cagcatttag tgcaagtact caaggaaaag ttgtagctct
     2941 tcaaatagca agaatgttaa aacaacaaga gccttta
//
LOCUS       DAOQAN010000072         2973 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_45827_length_2973_cov_2.324537, whole genome shotgun sequence.
ACCESSION   DAOQAN010000072 DAOQAN010000000
VERSION     DAOQAN010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45827_length_2973_cov_2.324537"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..748)
                     /locus_tag="PKO10_01840"
     CDS             complement(<1..748)
                     /locus_tag="PKO10_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HNW65933.1"
                     /translation="MKIAILFGGLSFEHEISIVSSIAMKDVLKDDLIYFYVDSKRDIY
                     EIPTNKINSKLFSSGEYKKFDKVYLKKGGFYKISGLFKKEQNIDFDVVLNLSHGGDGE
                     DGILSSVLDFYNIPFIAPRTEACVVSSNKFITKGYAKSVGVNVLDYKYFTKKDSVKVD
                     MFPVILKPVKLGSSIGVSIVKNQEELSYALDVAFEFDDAIIIEPFISGVKEYNLAGTK
                     VNGEFIFSIIEEPQKAEFLDFDKKYLDFSRT"
     gene            complement(765..1322)
                     /gene="ruvA"
                     /locus_tag="PKO10_01845"
     CDS             complement(765..1322)
                     /gene="ruvA"
                     /locus_tag="PKO10_01845"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HNW65934.1"
                     /translation="MIVGLVGRVVKKEPTSLQLNVGGVIYEVFVSLNCSSKIISDEIT
                     LEITEIIREDAHNLYGFLDSNEKKLFDTVIKINGVGPKVALAICSTFTPSSFAQIVTS
                     NDVNMLKRVPGIGPKGASRILVELSGFIVDGASSDEATNINIEASLALESLGFKKDIV
                     TTILKSCTSTTTAELVREALRKLQK"
     gene            1454..>2973
                     /locus_tag="PKO10_01850"
     CDS             1454..>2973
                     /locus_tag="PKO10_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNW65935.1"
                     /translation="MLDFITKKISSKIIFALFLLMFISSSIIVYSTTTKVTKDSIANA
                     KESLEMLNASIFQTLRNTMNTGDPALIQKAEDEAREIKGVKNLTVAKSKELMELYSVE
                     TPFTDDPQILKSFESKENQIIQKNDENGHTIRMIKPMIATTECMACHANQNVGDVIGV
                     MDLTFSLEETDKRIQSLLAEISFTSAVLGLLTIILIFFIVKKATNSIEILKEGFSNLL
                     HSNDTNITLKVKSNDEIGDVATLFNSYMDKVRDGLKQDEIVIEEANDVIEKTANGFFV
                     YKVHNTASNHHVEDLKNKLNVMIEKTKDTLDNINEALRQYAESKYDYVLKDDAIFGNL
                     GSLASGINLVGNNTSELLAIVMNTGNSLKDSTQTLSDTSLELKSSSSKQAASLEETAA
                     ALEEITATIQANTQSTIKMSKLAHELSISAQKGQELANQTAKSMDDINKEVSSINEAI
                     EVIDQIAFQTNILSLNAAVEAATAGEAGKGFAVVAQEVRNLANRSAEAAKEIKNLV"
BASE COUNT         1141 a          457 c          392 g          983 t
ORIGIN      
        1 aagttcttga aaaatctaaa tattttttgt caaaatctaa gaattcagct ttttgaggct
       61 cttcaataat tgaaaatata aactctccat ttacttttgt tccagctaaa ttatactctt
      121 taactccact aataaatggc tcaattataa tagcatcatc aaattcaaaa gcaacatcaa
      181 gagcataaga tagctcttct tgatttttaa caatagaaac tccaatagag cttcctagtt
      241 taactggttt taaaataact ggaaacatat caacttttac actatctttt tttgtaaaat
      301 atttataatc caatacattc acaccaacac tttttgcata accttttgtg ataaatttat
      361 tagagcttac aacacaagct tcagttcttg gagctataaa aggaatatta taaaaatcca
      421 aaacagatga taaaatacca tcttctccat ctcctccatg agatagattt aaaactacat
      481 caaaatctat attttgctct tttttaaata atccagaaat tttataaaaa ccaccttttt
      541 ttaaataaac tttatcaaat tttttatact caccactact aaaaagtttt gaatttatct
      601 tatttgttgg aatttcataa atatctcttt ttgagtcaac ataaaaatat attaaatcat
      661 cttttaaaac atctttcata gcaatagaac tcactattga tatctcatgc tcaaaactca
      721 aaccaccaaa aagtattgct attttcataa ttatttccct ttatctattt ttgtaatttt
      781 ctaagtgctt cacgaactaa ctcagctgta gttgtgcttg tacaagattt taaaattgtt
      841 gttacaatat cttttttaaa tcctaaactc tcaagagcca aagaagcttc aatatttata
      901 tttgtagctt catcactact tgctccatct acaataaagc cactaagttc aaccaaaatt
      961 ctacttgcac cttttggtcc aattccagga actcttttta gcatatttac atcatttgaa
     1021 gtgacaattt gtgcaaatga gctaggagta aaagttgaac aaatagcaag tgctactttt
     1081 ggaccaactc catttatttt tataactgta tcaaataatt tcttctcatt actatctaaa
     1141 aaaccatata gattatgtgc atcttctctt attatttctg ttatttcaag agttatttca
     1201 tctgaaataa tttttgaact acaatttaaa gatacaaaaa cttcatatat aacaccacca
     1261 acatttagtt gaagtgatgt tggttctttt ttaacaactc taccaactaa tcctactatc
     1321 acagcgttct cactttattt tttattataa tttttataaa gagtgcttaa tttagaaaac
     1381 taaactctta tttaatactt tttaaatatt ttaaagtata taataattcc acttaaaaaa
     1441 taagggttct aatatgcttg attttataac aaaaaagatt agtagcaaaa ttatatttgc
     1501 actattttta ctaatgttta ttagcagttc tattattgtt tactctacaa caaccaaagt
     1561 aacaaaagat tcgatagcaa atgcaaaaga gagtttagag atgctaaatg cttcaatatt
     1621 tcaaacctta agaaatacaa tgaacactgg agatccagca cttatacaaa aagcagaaga
     1681 tgaagcaaga gagataaaag gtgtaaaaaa tctaacagta gcaaaaagta aagagttaat
     1741 ggagctatac tctgttgaga caccatttac agatgaccct caaatattaa aaagttttga
     1801 atcaaaagag aatcaaatca ttcaaaaaaa tgacgaaaat ggtcatacta taagaatgat
     1861 aaaaccaatg attgcaacaa ctgagtgtat ggcttgtcat gcaaaccaaa acgtaggaga
     1921 tgtaattggt gttatggacc ttactttttc tcttgaagag acagataaaa gaattcaatc
     1981 acttttagca gagatatctt ttacatcagc tgttttagga ttacttacaa ttattctaat
     2041 attttttatt gtaaaaaaag ctacaaactc tatagagata ttaaaagaag ggtttagtaa
     2101 tctacttcat tcaaatgata caaatattac tttgaaagta aaatcaaatg atgaaatagg
     2161 agatgtagca acgcttttca actcttatat ggataaagta agagatgggt taaagcaaga
     2221 tgaaattgtc attgaagaag caaatgatgt aatagaaaaa actgcaaatg gattttttgt
     2281 atataaagtt cacaatacag cttcaaatca tcatgttgaa gatttaaaaa ataaactaaa
     2341 tgttatgatt gaaaaaacaa aagatactct tgataatata aatgaagcgc taagacaata
     2401 tgctgaatca aaatatgatt atgttttaaa agatgatgct atttttggaa atttaggttc
     2461 acttgctagt ggaataaatc tagtaggaaa taatacatca gaacttttag ctattgttat
     2521 gaatactgga aatagcttaa aagatagtac tcaaacactt tcagacacct ctttggagct
     2581 taaaagttct tctagcaaac aagctgctag tcttgaagag actgcagctg cacttgaaga
     2641 gataacggca acaattcaag ctaacactca atcaacaata aaaatgtcaa aacttgcaca
     2701 tgagcttagt atttcagcac aaaagggtca agaattagct aatcaaacgg ctaaatctat
     2761 ggatgatata aataaagaag ttagctctat aaatgaagct attgaagtaa tagaccaaat
     2821 agcatttcaa acaaatattc taagtctaaa tgctgctgtt gaagctgcaa ctgctggaga
     2881 agctggaaaa ggatttgcag ttgttgctca agaggtgaga aatctggcaa atagaagtgc
     2941 cgaagctgct aaagagataa aaaacttagt aga
//
LOCUS       DAOQAN010000073         2956 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_46229_length_2956_cov_2.825577, whole genome shotgun sequence.
ACCESSION   DAOQAN010000073 DAOQAN010000000
VERSION     DAOQAN010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46229_length_2956_cov_2.825577"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="PKO10_01855"
     CDS             <1..231
                     /locus_tag="PKO10_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014473124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HNW65936.1"
                     /translation="CLDECPVGAIVDNDDNPTGEDVYYVYKDKCVECVGHNDAPACAD
                     ACPTEGCIVWDEVGSSKIEKEDRGEVGEPVVD"
     gene            306..719
                     /gene="ndk"
                     /locus_tag="PKO10_01860"
     CDS             306..719
                     /gene="ndk"
                     /locus_tag="PKO10_01860"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002859435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HNW65937.1"
                     /translation="MERTLSIIKPDAVAKNVVGKILDRFESAGLRIAATKKLQLSKAD
                     AEAFYAVHAARPFFKDLVEFMISGPVVVSVLEGVNAMAKNRDLMGATNPKEAAAGTIR
                     ADFADSIDANAVHGSDSLENAAIEIDFFFAQREIC"
     gene            726..1103
                     /locus_tag="PKO10_01865"
     CDS             726..1103
                     /locus_tag="PKO10_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW65938.1"
                     /translation="MKIEFKKIPQEKKEFNTSLNSVKIEGTFCRISSSLVKIEASLIG
                     NIEIDCSRCGALDTLVVNEELKLLLSDGVFKGDEDEFLVIEIENSLIDFDEIIQSEVN
                     SIKSDYLYCKDCIADSSKFEQEF"
     gene            1119..1271
                     /gene="rpmF"
                     /locus_tag="PKO10_01870"
     CDS             1119..1271
                     /gene="rpmF"
                     /locus_tag="PKO10_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HNW65939.1"
                     /translation="MAVPKRRVSHSRSAMRRTHYKVALKRPVKDSDGSWKMPHMVNPN
                     TGEYKN"
     gene            1271..2293
                     /gene="plsX"
                     /locus_tag="PKO10_01875"
     CDS             1271..2293
                     /gene="plsX"
                     /locus_tag="PKO10_01875"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014768456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HNW65940.1"
                     /translation="MIKIAIDAMGGDFGPEPIIEGLILAIRNNNNFTAIAVGDKEQLS
                     KLIPPTFNDRIEILDTKDVISMHDSATDALKRKESTIYKAIELVKDGKADAVVSAGHS
                     GASMSLATLKIGRLKGVSRPAIATLMPTSENQNTLVLDVGANVDSDAKNLFQFAIMGQ
                     AYAQVVLRLDEPIIGLLSNGEEESKGNEVTKEAYKLISKVPNFAGNVEGNDIFKGTVD
                     VVVCDGFVGNILLKTAEGVADTISKIMKKDLKRSLISIAGAVLMRKVFKNLKLKVDYA
                     EYGGAPLLGVKAPVIISHGKSNPKAIKNAIFQAINSASSNLDSVIEQRLEKYLINESS
                     SKEPTL"
     gene            2303..>2956
                     /locus_tag="PKO10_01880"
     CDS             2303..>2956
                     /locus_tag="PKO10_01880"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013136545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase 3"
                     /protein_id="HNW65941.1"
                     /translation="MKYAAFRSIGAYIPSKIMTNADFEKIIDTNDEWITKRTGIKERR
                     IAEDNEASSDLGAKAAEVAIKRANISKDEIDLIICATVTPDFLCMPSTACLIASKLDI
                     KDVMAFDVSAACTGFVYILNIAKAFIESGLKKNVLIVGAEKYSSILNYEDRTTCFIFG
                     DGAGAAIISATDDKSEAIIDISCSSDGTYEDLIKTTGGGSKHPCSQEVVDAKMACISM
                     "
BASE COUNT         1117 a          332 c          603 g          904 t
ORIGIN      
        1 tgtttagatg agtgtccagt tggagcaata gtagataatg atgataaccc aacgggtgaa
       61 gatgtatatt atgtgtataa agataaatgt gtagagtgtg ttggtcacaa tgatgctcca
      121 gcatgtgcag atgcatgtcc tactgagggt tgtatagttt gggatgaagt tggttctagc
      181 aagatagaaa aagaggatag aggagaggtt ggagaacctg tagtagatta ataaagttat
      241 aaaacttgct tttttatctt tttttagata aaatccgcga ctaaaaaaac aaagaggatt
      301 agattatgga aagaacatta tcgataataa aaccagatgc agttgcaaaa aatgtagttg
      361 gtaaaatttt agatagattt gaaagtgctg gattaagaat tgcagctaca aaaaaactac
      421 aattaagcaa agctgatgct gaagcttttt atgcagttca tgcagctaga cctttcttta
      481 aagatttggt tgaatttatg attagtggac ctgttgtagt ttcagtttta gaaggtgtta
      541 acgcaatggc aaaaaataga gatttaatgg gtgcaacaaa ccctaaagaa gctgcagctg
      601 gaacaattag agcagatttt gcagattcaa ttgatgcaaa tgcagtacat ggaagtgact
      661 ctttagaaaa tgcagctatt gaaattgatt tcttttttgc acaaagagaa atttgttaat
      721 ataagatgaa aatagagttt aagaaaatac ctcaagaaaa aaaagagttc aatacttctt
      781 taaattcagt taaaattgaa ggtacttttt gtagaatttc atcatcgtta gtaaaaattg
      841 aagcaagttt aataggaaat attgaaatag attgctcaag atgtggagct ttagatactt
      901 tggttgtaaa tgaagagtta aaacttcttt taagtgatgg agtatttaaa ggagacgaag
      961 atgaattttt agtaatagaa attgaaaata gtttaattga ttttgatgaa ataattcaaa
     1021 gtgaagtaaa tagtataaaa agtgactact tgtactgtaa agattgtata gcagatagta
     1081 gcaaatttga acaagaattt taaaatagga gaataattat ggcagtacca aaaagaagag
     1141 tatctcattc aagatcagca atgagaagaa ctcattataa ggttgcatta aaaagaccag
     1201 taaaagatag tgatggttct tggaaaatgc ctcatatggt taacccaaac actggtgaat
     1261 acaaaaacta atgataaaaa tagcaataga tgcaatggga ggggactttg gtcccgaacc
     1321 aataatagaa gggcttattc tagccattag aaacaataac aattttacag ctatagctgt
     1381 aggagataaa gaacaacttt caaaacttat cccacctaca tttaatgata gaatagagat
     1441 tttggataca aaagatgtta ttagtatgca tgatagtgca actgatgctc taaaaagaaa
     1501 agagtcaact atttataaag caatagagtt agtaaaagat ggtaaagcag acgctgttgt
     1561 atctgctggt cactctggag cttccatgtc tttagctaca ttaaaaatcg gaagattaaa
     1621 aggtgtttca aggcctgcta ttgcaacact tatgcccacg agcgaaaatc aaaatacttt
     1681 agtattagat gttggtgcaa atgtagatag tgatgcgaaa aacttattcc aatttgcaat
     1741 tatgggtcaa gcttatgcgc aagttgtttt aagacttgat gaaccaataa ttggactatt
     1801 aagcaatggt gaagaagaga gcaaaggaaa tgaagtaact aaagaggctt ataagttaat
     1861 ttctaaagtt cctaacttcg ctggaaatgt tgaaggaaat gatatcttca aaggaacagt
     1921 agatgttgtt gtttgtgatg gctttgtagg taatatccta ctaaaaacag ctgagggcgt
     1981 tgctgatact attagtaaaa ttatgaagaa agatttaaaa agatctctta tatcaatagc
     2041 tggtgcagtt ttgatgagaa aggtttttaa aaatcttaaa ttaaaagttg attatgctga
     2101 gtatggtggt gctccacttc ttggagtaaa agcaccagtg ataatatctc atggtaaatc
     2161 aaatccaaaa gctataaaaa atgcaatttt tcaagcaatt aatagtgcta gctcaaattt
     2221 agattctgta attgagcaaa gattagagaa atatttaata aatgaaagta gtagtaaaga
     2281 acctactctt taaggaaatg atatgaaata tgcggctttt aggtcaattg gtgcttatat
     2341 tccatcaaaa ataatgacta atgcagattt tgaaaaaata attgatacaa atgatgagtg
     2401 gattacaaaa agaacaggta taaaagagag aagaatagct gaagataatg aagcatcttc
     2461 tgatttaggt gcaaaagctg cagaagttgc aataaaaaga gcaaatattt ctaaagatga
     2521 gatagattta ataatttgtg caacagttac accagatttt ttatgtatgc cttcaactgc
     2581 ttgtttgatt gcttcaaaat tagatataaa agatgttatg gcttttgatg taagtgctgc
     2641 atgtacaggt tttgtatata ttttaaatat tgcaaaagct tttattgaat caggtttaaa
     2701 gaaaaatgtt ttaatagttg gagccgaaaa atatagttca attttaaatt atgaagatag
     2761 aacaacttgt tttatatttg gagatggcgc aggtgccgca ataattagtg ctacagatga
     2821 taaaagtgaa gcaataattg atattagttg ttctagtgat ggaacttacg aagatttaat
     2881 taaaactact ggtggtggaa gtaaacatcc atgtagtcaa gaagttgtag atgcaaaaat
     2941 ggcttgtatc tctatg
//
LOCUS       DAOQAN010000074         2948 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_46417_length_2948_cov_3.238507, whole genome shotgun sequence.
ACCESSION   DAOQAN010000074 DAOQAN010000000
VERSION     DAOQAN010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46417_length_2948_cov_3.238507"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="PKO10_01885"
     CDS             <1..402
                     /locus_tag="PKO10_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014475330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW65942.1"
                     /translation="VNSSLILIFYILFVLIVILNIQKKSILLLFFNILFVYISYFAVT
                     YFFSTYIYELPTHQCPFCMLQSEYYFIGYFIWSALFLGLFFSICSVVFYRNNSLDIYK
                     FYKLSLIFTTIFVFLVTFFVLKYYVVNGVFL"
     gene            complement(437..721)
                     /locus_tag="PKO10_01890"
     CDS             complement(437..721)
                     /locus_tag="PKO10_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW65943.1"
                     /translation="MKKILLSLAAASLLFVGCTEDKKPAETKVEAPKTETVAPAQEKK
                     EEVAAPKEETKETVANTEEKEVKATEEDKKEEAETTKEESKETATEADKK"
     gene            complement(867..942)
                     /locus_tag="PKO10_01895"
     tRNA            complement(867..942)
                     /locus_tag="PKO10_01895"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(907..909),aa:Cys,seq:gca)
     gene            complement(988..1076)
                     /locus_tag="PKO10_01900"
     tRNA            complement(988..1076)
                     /locus_tag="PKO10_01900"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1039..1041),aa:Ser,seq:tga)
     gene            complement(1086..1172)
                     /locus_tag="PKO10_01905"
     tRNA            complement(1086..1172)
                     /locus_tag="PKO10_01905"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1136..1138),aa:Leu,seq:taa)
     gene            1361..2948
                     /gene="ilvD"
                     /locus_tag="PKO10_01910"
                     /pseudo
     CDS             1361..2948
                     /gene="ilvD"
                     /locus_tag="PKO10_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011138094.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
BASE COUNT          959 a          383 c          548 g         1058 t
ORIGIN      
        1 gttaattcaa gcttgatact tatattttat atactttttg ttttgattgt tattttgaat
       61 attcaaaaaa aatctatttt gcttcttttt tttaatatac tatttgttta tatatcttat
      121 tttgcagtaa catacttttt ttcaacttat atatatgaac ttccaacaca tcaatgtcca
      181 ttttgtatgc ttcaatctga gtattatttt ataggatatt ttatatggtc ggcacttttt
      241 ttaggacttt ttttctcaat ttgttcggta gttttttata gaaataatag tttagatatt
      301 tataagtttt ataaactatc tttaatattt acaacaattt ttgttttttt ggtaactttt
      361 tttgttttaa aatattatgt agtaaatgga gtttttttat aaatccgtga ttttacacgg
      421 atttatattt ttataattat tttttgtcag cttcggttgc tgtttctttt gattcttctt
      481 ttgttgtttc agcttcttct tttttatctt cttccgtagc ttttacttct ttttcttcag
      541 tattagctac tgtttctttt gtctcttctt ttggagcagc tacttcttct tttttctctt
      601 gagctggtgc tacagtttca gttttaggcg cttcaacttt tgtctcagct ggttttttat
      661 cttctgtaca acctacaaat aataaagatg ctgctgctaa tgataataat atttttttca
      721 ttttaaacct ttttatgtaa ttttttgttt ataaaaacaa attgcggaat catatcaaaa
      781 cgtttgtgaa ataattgtgt aataaattaa ttttttttag aaaatttact aacaatatat
      841 aaagtatgat atgtggaaat ttgaaatgga ggagctagtc ggactcgaac caacgcatgt
      901 cggatttgca atccgaggca ttaccagctt tgctatagct ccaaaaaaat tatgatattt
      961 ttgtgtattt acttttataa aaaagagtgg cagaggacaa gggattcgaa ccctcggagg
     1021 ctttcacctc gccggttttc aaaaccggta caatcgacca actctgtcaa tcctctacaa
     1081 cttaatggtg cgaatggtga gaatcgaact cacactccga aaccggaacg ggattttaag
     1141 tcccgcgcgt ctacctattc cgccacactc gcacaaaatt taagaacctt tttaagcttt
     1201 cacttaaatt ggactggaat tatagtagct ttttttttat ttgtcaagag ttttttaaaa
     1261 aaatttaaaa attttaattt ttatcccatt taatagagat tgaaaatgaa tatttgtata
     1321 atcactaata tttattttta caagtttaaa aggattgaaa ttgagaagtg atgaagtaaa
     1381 aaaaggttat tcaagagcac cacatagatc tcttttgaga gcaacaggtc ttaaagatga
     1441 ggattttaat aaaccattta ttggagttgc aaactctttt atagagatta ttccaggaca
     1501 ctttttctta gataaagttt caaaaatcat aaaagatgaa attagaaaaa atggttgtgt
     1561 acctttcgaa ttcaacacta ttggagttga tgatggaatt gctatggggc atgatggtat
     1621 gcttttttca ctaccaagta gagaattaat tgcaaactct attgaaactg ttatgaatgc
     1681 tcataaactt gatgcaatga ttgctattcc aaattgtgac aaaatagttc ctggtatgat
     1741 aatgggtgct ttaagagtaa atgttccaac tgtttttgta agtggtggag ctatgcaaaa
     1801 aggttataaa aaagacggaa caccaataga tttggcaact gcttttgaag ctgttggaaa
     1861 gtttgaagct ggagaaatca gtgaagaaga gttaaaagat attgagtgta atgcatgtcc
     1921 aggtggagga agttgttctg gaatgtttac agcaaactct atgaatactc ttatggaagc
     1981 tatgggaatt gcactgcctg gaaatggaac aattctagca cttacaaaag agagagaaga
     2041 gttatataga aaagctgcta aaagggtttg tgaattagct cttgataaga caaatagcga
     2101 gaagttcaga ttaaaaaata ttttaaatga aaaagctgta agaaatgctt ttgctgttga
     2161 tatggcaatg ggtggaagtt caaatactgt tttacatatg ttggcaattg caaaagaggc
     2221 tggagtagat tttaaccttg aagatataaa tgccatatca aaaagagttt cacatattgc
     2281 aaaaatatca ccatctaacg actgttcata tggaagatat aaataaagct ggtggagtaa
     2341 gtgctgttat gcatgaaatg agtaaaagag gagatgatat tcttttagat aatcttacag
     2401 ttacaggtga aactttaaaa gagagaataa aagattcaaa aattcttgat actactatta
     2461 ttcacactat agataaccct tactctgaag ttggtggatt agctattctt tatggaaatc
     2521 tagcacagca aggagcagtt ataaaaactg ctggaattac aggagctaga gctatgagtg
     2581 gaaaagctgt ttgttttgat ggacaagctg aagctataaa aggaatagtt ggcggaaaag
     2641 taaaagctgg cgatgttgta gtaattagat atgaaggtcc aaaaggtggt cctggaatgc
     2701 aagagatgct tgcacctaca agtttaatta tgggaatggg acttggtgat aaagttgcac
     2761 ttattacaga tggaagattt agtggagcaa caagaggtgc tagtatcgga cacgttagtc
     2821 cagaagcagc tgaaggtgga atgataggtt tattacaaga tggagatatt ataaatattg
     2881 atgttgataa atatatttta agcgttgatt taagtgatga tgaaatagca cgtagaaaag
     2941 caaatttc
//
LOCUS       DAOQAN010000075         2948 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_46420_length_2948_cov_2.314552, whole genome shotgun sequence.
ACCESSION   DAOQAN010000075 DAOQAN010000000
VERSION     DAOQAN010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46420_length_2948_cov_2.314552"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1323
                     /locus_tag="PKO10_01915"
     CDS             <1..1323
                     /locus_tag="PKO10_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase flavoprotein subunit"
                     /protein_id="HNW65944.1"
                     /translation="VFKQTTNAVICEGIGAAIALETGIATLGNMEAVQFHPTPIVPSG
                     ILLTEGCRGDGGILRDVDGHRFMPDYEPEKKELASRDVVSRRMIEHIRNGKGVPSPYG
                     YHVWLDISILGREHIEKNLRDVQEICQIFNGIDPADEGPKGWAPVLPMQHYSMGGIRT
                     KPTGESQNLKGLFACGEAACWDMHGFNRLGGNSVSETVVAGMIIGNYFADFCLENDVT
                     IPTSVVQKFVDEQEAYIDEILSYNGNEDIFKIKNRMKELMDEKVGIFRSGEPLKEAVE
                     ELKDLLAKTKKITVKSKEKAGNPELEEAYRVPKMLKIALCVAKGARERTESRGAHYRE
                     DFLMRDDKNWLNRTLTSWPNKNDMEPTVTYEPLDIMKMEMPPAFRGYGAKGMIIEHEL
                     SAVRQEQVDSITEKMQNEGKDRHEIQDALMPFSLPMNYKEKNERAGDK"
     gene            1323..2054
                     /locus_tag="PKO10_01920"
     CDS             1323..2054
                     /locus_tag="PKO10_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur subunit"
                     /protein_id="HNW65945.1"
                     /translation="MSAQKGREITISVLKYNPRSKVSKPHFVEFKLEETPGMTLFIAL
                     NLIRENYDADLSFDFVCRAGICGSCGMMVNGKPALACRTLIANYPTGKLQLMPMPAFE
                     LIKDLSVNTGKWMDAMSKRVESWIHTNEEVDISKMEQRVDPKVANDTFELDRCIECGI
                     CVASCGTMLMRPNFVGPVGLNRIARFDIDPHDNRTAEDFYELVGDDDGVFGCMSLMAC
                     EDHCPKHLPLQNKIAYLRRKLVALR"
     gene            2149..>2948
                     /locus_tag="PKO10_01925"
     CDS             2149..>2948
                     /locus_tag="PKO10_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HNW65946.1"
                     /translation="MSLSNDYFLLYHGISFQKNLELEPLDVILDDNTFTIFALIISAN
                     RKNYDKYLNLNSFKKLCEQINIKTPSNINEFHNIQNSIVELIHEKSSKNLITTLIESF
                     EYLNNQNILNTEGFKKLISLFENKNIEDSDLEHIKKMESESKTPFKDLKVIIENTLID
                     LKKELKSKEILIELEDINYYMNNQKFSIGITGVMNAGKSTMLNALMGKEILGSAVVPE
                     TANLTIVKHNPTDNAKVFYWNKKEWQKIEDSANSLESMRDFVDETNRV"
BASE COUNT         1137 a          356 c          562 g          893 t
ORIGIN      
        1 gtatttaaac aaactacaaa tgctgtaatt tgtgaaggta ttggagctgc tatcgcactt
       61 gagactggaa ttgctacttt aggaaatatg gaagctgttc agttccaccc aacaccaatc
      121 gtaccatctg gtattttatt aactgaaggt tgtagaggag atggtggaat cttaagagac
      181 gttgatggtc acagatttat gccagattat gagccagaga aaaaagaact tgcttcaaga
      241 gacgttgttt caagaagaat gattgagcat attagaaatg gtaaaggtgt tccttctcct
      301 tatggatacc acgtatggtt agatatttct attcttggtc gagagcatat tgagaaaaac
      361 ttaagagacg ttcaagaaat ttgtcaaatt ttcaacggaa tagatccagc tgatgagggt
      421 ccaaaaggtt gggcaccagt tcttccaatg caacactact ctatgggtgg aattagaaca
      481 aaaccaactg gtgagtcaca aaacctaaaa ggtctatttg cttgtggaga agctgcttgt
      541 tgggatatgc acgggtttaa tagacttgga ggaaactcag tttctgaaac agttgttgct
      601 ggtatgatta ttggaaacta ttttgcagat ttctgtttag aaaatgatgt aacaattcca
      661 acttctgttg ttcaaaaatt tgttgatgag caagaagcat atattgatga aattctatct
      721 tacaatggaa atgaagatat tttcaaaatt aaaaatagaa tgaaagagct tatggatgag
      781 aaagttggta tctttagaag tggtgaacca ctaaaagagg ctgttgaaga gttaaaagat
      841 ctattagcta aaactaaaaa aattacagtt aaatcaaaag aaaaagctgg aaatccagaa
      901 ttagaagaag cttatagagt tccaaaaatg ttgaaaattg cactttgtgt agctaaaggt
      961 gcaagagaaa gaacagaatc tagaggtgct cactatagag aagatttctt aatgagagat
     1021 gataaaaatt ggttaaatag aactcttaca tcttggccaa ataaaaatga tatggaacca
     1081 actgttactt atgaaccact tgatattatg aaaatggaaa tgcctcctgc atttagagga
     1141 tatggtgcga agggtatgat tattgaacat gaattatcag cagttagaca agaacaagtt
     1201 gatagtataa ctgagaaaat gcaaaatgaa ggtaaagata gacatgaaat tcaagatgca
     1261 ttaatgccat ttagtttacc tatgaactac aaagagaaaa atgaaagagc aggagataaa
     1321 taatgagcgc acaaaaaggt agagaaatta caatttcagt tcttaaatat aatcctagaa
     1381 gtaaggtttc aaaacctcac ttcgtagagt ttaagctaga agagactcca ggtatgactc
     1441 tttttattgc attaaactta attagagaaa actatgatgc tgatttatct tttgacttcg
     1501 tatgtagagc tggaatttgt ggaagctgtg gaatgatggt aaatggaaaa cctgcacttg
     1561 cttgtagaac actaattgca aactatccaa caggaaaatt acaattaatg cctatgcctg
     1621 catttgaact aatcaaagat ttatcagtaa acacaggtaa atggatggat gctatgagca
     1681 aaagagttga atcatggatt catacaaatg aagaagttga tatttcaaaa atggagcaaa
     1741 gagttgatcc aaaagtagca aatgatacat ttgaattaga tagatgtatt gagtgtggta
     1801 tttgtgttgc ttcttgtgga acaatgctaa tgagaccaaa ctttgttgga ccagttggat
     1861 taaatagaat tgcaagattt gatatagatc cacatgataa tagaactgcg gaagatttct
     1921 atgagttagt tggagatgat gatggagtat ttggttgtat gtcattaatg gcttgcgaag
     1981 accattgtcc aaaacattta ccactacaaa ataaaattgc ctacttaaga agaaaattag
     2041 tagcacttag atagttattt atgaaggttt taccttcata aatctaaata atagaaaaag
     2101 ttaaatatca ttatggatat ttaacttttt ttatttagga gagaattaat gagtttatca
     2161 aatgactact ttttgttgta ccacggtatt agctttcaaa aaaatttaga gcttgagcct
     2221 cttgatgtaa ttttagatga taacactttt acaatattcg cattaattat tagtgccaat
     2281 agaaaaaact atgataaata tttaaattta aactccttca aaaaactttg tgagcaaata
     2341 aatattaaaa ctccatcaaa tataaatgag tttcacaata tacaaaacag tattgttgaa
     2401 ttaattcatg aaaaaagttc taaaaattta attactactt taattgagtc atttgagtat
     2461 ttaaataatc aaaatatact aaatacagaa ggttttaaaa aactaatatc actatttgaa
     2521 aataaaaata tagaagatag tgatttagaa catataaaaa agatggaaag tgagagtaaa
     2581 actccattta aagatctaaa agttattatt gaaaatactt taattgattt aaaaaaagag
     2641 ctaaaaagca aagaaatttt aatagaactt gaggatataa actattatat gaataatcaa
     2701 aagttctcta ttggaataac aggtgttatg aatgcaggaa aatctacaat gctaaatgca
     2761 cttatgggaa aagagatttt agggagtgcc gttgttcctg agactgcaaa tttgactatt
     2821 gtaaaacata atccaactga taatgcaaaa gttttctatt ggaataagaa agagtggcaa
     2881 aaaatagaag atagtgcaaa tagtttagaa agtatgagag attttgtaga tgaaacaaat
     2941 agagtttt
//
LOCUS       DAOQAN010000076         2938 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_46680_length_2938_cov_2.563649, whole genome shotgun sequence.
ACCESSION   DAOQAN010000076 DAOQAN010000000
VERSION     DAOQAN010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46680_length_2938_cov_2.563649"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1191
                     /locus_tag="PKO10_01930"
     CDS             <1..1191
                     /locus_tag="PKO10_01930"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HNW65947.1"
                     /translation="TQNLSQIVVNNEDRQNIENQIETSTVLKDSLNFENILIVEDEVA
                     NSDTIKNETVKNENKLNSQNILIVENEVVKTENNTKVDQKLSLMDQLIQTNSKKDITT
                     KIEESTTSNPQSVEQINKNSKDVASNIFLAEQKNSLNNQLLFNKNEAVNILKNASSIE
                     DIEKSANILDLDANNLEVEQNISSESLNDLSVDEKEILDRKNILNSILNEKNIRSVDV
                     RNLITNSIEASKALLEDTINILDDKILDIQPNLVNSIQSRIVGAKQQMASMMSDVARQ
                     MYENYKPPVTVFRMNLNPGDLGTISVLMKQDKSSGLTINMSVSNIATLELLMENQNML
                     RNSLAKTFNDSANFNLDFSKGEDGQSQGDSSSNQNQRDKRDSNTQEILRLKKENKDYE
                     EKND"
     gene            1197..1679
                     /locus_tag="PKO10_01935"
     CDS             1197..1679
                     /locus_tag="PKO10_01935"
                     /inference="COORDINATES: protein motif:HMM:NF013478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HNW65948.1"
                     /translation="MEEFFSLLNEDVLVKFLLLFARVVSFVAFMPVFGHTAISVTVRV
                     AFAFYLVIFLFPFIGDVKYISESSFIVSLLSEITLGLVAAMLVNIIFSSVKIIGEFVE
                     YSTALSMAMMFDPTTGSQEGLVAKLLFWISLMLFFQTGMYEMTLVFFSISFFLNPWFY
                     "
     gene            1694..1981
                     /locus_tag="PKO10_01940"
     CDS             1694..1981
                     /locus_tag="PKO10_01940"
                     /inference="COORDINATES: protein motif:HMM:NF013478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HNW65949.1"
                     /translation="MIHLGTFDIFSINGIKLAIGEINRMFAFAFTFALPLFFIGFILD
                     VYYGYGTKSMPAFSPFIITFQLKFALIFLFLIFGLEIFFDAFTNYFISKFE"
     gene            1990..>2938
                     /gene="flhB"
                     /locus_tag="PKO10_01945"
     CDS             1990..>2938
                     /gene="flhB"
                     /locus_tag="PKO10_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HNW65950.1"
                     /translation="MADDEEKTEEPTSKKIEDARAEGNVGKSAEVAGAVILTLGSVYL
                     IFLSGFMFLEIKKVMLYIYGFIGQEIDESTYFTITVTVATTLVKALSPIYLLVFVLAL
                     ASNWMQFGFIATPLKFDLQKLDPIKGLGNVFSLKKLMEALKLTLKLAVIVWVMFLLFS
                     LTYQDFLTMMNKELNATIEAMIDLIIIFVFTILFIIIIFAIIDFYFSKHYYMKSLKMS
                     KQEIKDEYKNMEGDPQVKGRIRRIQMQMAQKRMMSSVPDADVVITNPTHYAVALKYDS
                     SKNQAPLVVAKGIDFLALRIKEIAKENSVTIIENPSLARA"
BASE COUNT         1083 a          324 c          482 g         1049 t
ORIGIN      
        1 actcaaaatt tgtctcaaat agtagtaaat aatgaagata gacaaaatat agaaaatcaa
       61 attgaaactt ctacagtttt aaaagatagt ttaaattttg aaaatatttt gattgttgaa
      121 gatgaagttg caaatagtga tactatcaaa aatgaaactg taaaaaatga aaacaaatta
      181 aattcgcaaa atattttaat tgttgagaat gaagttgtaa aaactgaaaa caatacgaaa
      241 gtagatcaaa aattatctct tatggatcaa ttaattcaaa caaacagtaa aaaagatatt
      301 acaacaaaaa tagaggaatc tactacttca aatcctcaaa gtgtagagca aattaataaa
      361 aattcaaaag atgttgcctc aaatattttt ttagctgagc aaaaaaatag tttgaataat
      421 caactacttt ttaataaaaa tgaagcagtg aatattttaa aaaatgcttc aagtattgaa
      481 gatatagaaa aaagtgcaaa tattttagat ttagatgcaa ataatttaga agttgaacaa
      541 aatatctcta gtgagagttt aaatgattta agtgtagatg aaaaagagat tttagataga
      601 aaaaatattt taaatagtat tttaaatgaa aaaaatataa gaagtgtaga tgttagaaat
      661 cttataacaa actcaataga ggcttcgaaa gcccttttgg aagatactat aaatatttta
      721 gatgataaaa ttttggatat tcaaccaaat ttggtaaatt caattcaatc aagaattgtt
      781 ggagcaaaac agcaaatggc atctatgatg tctgatgttg caagacagat gtatgaaaac
      841 tataaaccgc ctgttactgt ttttagaatg aatttaaatc ctggagattt aggaactatt
      901 tcagttttaa tgaaacaaga taagtcaagt ggattaacta ttaatatgag tgtttctaat
      961 attgcaacac tagagctttt aatggaaaat caaaatatgt taagaaactc attggcaaaa
     1021 acatttaatg atagtgcaaa ctttaatctt gatttctcaa aaggtgaaga tggtcaaagt
     1081 caaggcgact cttcaagtaa tcaaaatcaa agagataaaa gagattcaaa cactcaagag
     1141 atattaaggc ttaaaaaaga gaacaaagat tacgaagaga aaaacgatta aatgtaatgg
     1201 aagagttttt ctcactttta aatgaagatg ttcttgttaa atttcttctt ctttttgcaa
     1261 gagtagtatc ttttgtagcg tttatgccag tttttggaca tacagcaata agtgttactg
     1321 taagagttgc ttttgctttt tatttagtaa tttttctttt tccttttatt ggtgatgtta
     1381 aatatattag tgaatcttca tttatagtat ctttattatc tgaaattact ttgggattag
     1441 ttgctgcaat gcttgtaaat ataatatttt catctgttaa gattataggt gaatttgttg
     1501 agtattcaac tgctttatct atggctatga tgtttgaccc aacaacaggt tctcaagaag
     1561 gtttggtagc aaaactactt ttttggatat cattgatgct atttttccaa acaggaatgt
     1621 atgaaatgac attggttttt ttctcaatct cctttttctt gaatccgtgg ttttattagc
     1681 aaaaagtttc tctatgatac atttaggaac ttttgatata ttctctataa atggtataaa
     1741 acttgctatt ggtgagataa atagaatgtt tgcctttgca tttacttttg ctttacctct
     1801 attttttata ggatttattt tggatgttta ttatggttat ggtacaaagt caatgccagc
     1861 attttctcca tttattataa cttttcagtt gaagtttgct ttaatatttt tgtttttaat
     1921 atttggttta gagatatttt ttgatgcatt tacaaactat tttattagta aatttgagta
     1981 ggaagtttta tggctgatga tgaagaaaaa acagaagaac ccacatccaa gaagatagaa
     2041 gatgccagag ctgaaggaaa tgtaggtaaa tctgctgaag tagctggtgc tgttatttta
     2101 actttgggtt ctgtttattt aatatttctt tcaggtttta tgtttttaga gataaaaaaa
     2161 gtgatgttgt atatctatgg ttttatagga caagaaatag atgaaagcac atattttaca
     2221 attactgtta cagttgcaac aacacttgta aaagctttgt ctcccatata tttgttagtt
     2281 tttgttttag cacttgctag taactggatg cagtttggat ttattgcaac tcctttaaaa
     2341 tttgatttac aaaaactaga tccaataaag ggactgggaa atgtttttag cctaaaaaaa
     2401 ttgatggagg ctcttaaatt aacccttaaa ttagctgtta tagtttgggt tatgttttta
     2461 cttttttcat taacatatca agattttttg acaatgatga ataaagagct aaatgcaact
     2521 atagaagcta tgatagactt gattattata tttgttttta ccattttgtt tattattatt
     2581 atttttgcta taatagattt ctatttttct aaacattatt atatgaaatc tttgaaaatg
     2641 agtaagcaag agataaaaga tgagtataaa aatatggaag gagaccctca agttaaggga
     2701 agaattcgta gaattcagat gcaaatggca caaaaaagaa tgatgagtag cgttcctgat
     2761 gcagatgttg ttattacaaa tccaacacac tatgctgttg ctttaaaata tgatagttca
     2821 aaaaatcaag ctcctttagt tgttgcaaaa ggtatagact ttttagcttt aagaataaaa
     2881 gagatagcaa aagagaatag tgtaactata atagaaaatc caagtctagc acgggctt
//
LOCUS       DAOQAN010000077         2929 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_46878_length_2929_cov_2.770355, whole genome shotgun sequence.
ACCESSION   DAOQAN010000077 DAOQAN010000000
VERSION     DAOQAN010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46878_length_2929_cov_2.770355"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1124)
                     /locus_tag="PKO10_01950"
     CDS             complement(<1..1124)
                     /locus_tag="PKO10_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsS family sensor histidine kinase"
                     /protein_id="HNW65951.1"
                     /translation="MIRNISISTFVNIIFTLAFVSIFLTFAMFIRYDKERHDLSLQNR
                     YEMIAENFLILFQDHPNAQRLNELYKKFNVKPIEDRDRKLEIINNAQELKITQNYLGT
                     YRVYRFDDMYYIYVQRYGYNIILKDTKHHNYNFAFIIAGFVLSLIIFIFLYEILNRKL
                     RPLKLLNRQIIEFSNGNKDIKLEYKSNDEVGTIAKNFNEAINIINNQSKSKDLFMRNM
                     MHELKTPITKAMFIAETLEDDKTRENLQRAFRRMDDIIKELATVEKLTSKNTMIYKEK
                     INFLSIFKKTTDLMLINPDNIEQKIEDFWFEADIYMISIALKNLIDNAIKFSTNKKAI
                     IEANKSFIKVISQGEPLKNELSFYTDAFYQEDKRSSGFGLG"
     gene            complement(1121..1798)
                     /locus_tag="PKO10_01955"
     CDS             complement(1121..1798)
                     /locus_tag="PKO10_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNW65952.1"
                     /translation="MIKILMIEDDLELAQIITDYLKSFDIEVTTTDSPYNGLSMLNLS
                     KEYQLLILDLTLPEIDGLELIPKIREKSQIPIIISSARDDILDKVMGLERGADDYLPK
                     PYNPRELQARIKTILKRVDNKLDDKKSQQDKVFELKESDMQILFKGESLVLTLAEYDI
                     LKLLIQRNHGVVSREDFIYTSDNIEDESSLKNIDVIISRIRTKLANIDNSKQYIKSVR
                     GIGYQLI"
     gene            complement(1811..>2929)
                     /locus_tag="PKO10_01960"
     CDS             complement(1811..>2929)
                     /locus_tag="PKO10_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNW65953.1"
                     /translation="GSGVIVTKDGYIVTNDHVIENAEEINVTIGDDPTEYSAKLIGKD
                     SDSDLAVVKIDINKPLVPIKLGDSSSLLVGDVTFAIGNPFGVGNTVTMGIISALNKNR
                     VGINRYENYIQTDASINPGNSGGALVDSRGALIGINTAILSKSGGNDGIGFAIPVEMV
                     KDVVGKLVSDGKVIRGYLGVVIVDLDKDSQQVYKRKEGAIILDISNDTPAAKYGLKRG
                     DLVYAINGKETKDRNSLQNAIASFKPNEKVKLEIERDKKDIVVEIVLADRSIISGVQP
                     DSDHTILNGLKVSPINAEIIKKYRLPLESMGILITDVEPKSKAERSGFLAGDIIIQIE
                     DVEIKNYSNIETALKRYNNQHKRVYINRYGQTLMFVIQ"
BASE COUNT          946 a          484 c          296 g         1203 t
ORIGIN      
        1 ccaagtccaa aaccgctact tcttttgtcc tcttgataaa aagcatctgt ataaaaagag
       61 agttcatttt tcaaaggttc accttgtgat attactttta taaaactctt gtttgcttct
      121 ataattgctt ttttatttgt tgaaaatttt attgcattgt ctatgagatt ttttaaagca
      181 attgatatca tatatatatc agcttcaaac caaaaatcct ctattttttg ctcaatatta
      241 tctggattta taagcattaa atctgttgtt tttttaaaaa ttgataaaaa atttatcttc
      301 tctttataaa tcatagtatt ttttgaagta agtttttcaa cagttgctag ctcttttata
      361 atatcatcca ttcttctaaa tgctctttga agattttctc ttgttttatc atcttctaaa
      421 gtttcagcaa taaacatagc ttttgtaata ggagttttaa gttcatgcat catatttctc
      481 ataaacaaat cttttgattt tgattgatta tttataatat ttatagcttc attaaaattt
      541 ttagcaattg tgcctacctc atcattactt ttatattcca attttatatc tttatttcca
      601 ttggaaaatt caataatttg tctgtttaaa agcttcagcg gtcgcaattt tctatttaaa
      661 atttcatata aaaatataaa tataatcaaa gaaagaacaa atccagcaat tataaatgca
      721 aaattgtagt tatgatgttt tgtatctttt aaaataatat tgtagccata tcgttgcaca
      781 tatatgtaat acatatcatc aaacctataa actctataag ttccaagata attttgtgta
      841 atttttaact cttgagcatt gtttataatc tctagttttc tatctctgtc ttctattggt
      901 tttacattga attttttata aagttcattt aatctttgag catttggatg gtcttgaaaa
      961 agtattaaaa aattttcagc aatcatctca tatctatttt gtaaacttaa atcatgtctc
     1021 tctttgtcat atctaataaa catagcaaag gttaaaaaaa tggatacaaa agctaatgta
     1081 aaaattatat ttacaaaagt tgatatagag atatttctta tcatattaac tgataaccaa
     1141 tacctcttac tgattttata tattgtttag agttatctat atttgctaat tttgttctta
     1201 ttcttgaaat gattacatct atatttttta aagatgattc atcttctata ttgtcacttg
     1261 tataaataaa atcttctctt gaaacaacac catgatttct ttgaataaga agttttaaaa
     1321 tatcatattc agccagtgtc aataccaaag attcaccttt aaaaagaatt tgcatatcac
     1381 tctcttttag ctcaaaaact ttatcttgtt gtgatttttt atcatcaagt ttattatcaa
     1441 ctctttttaa aattgttttg attctagctt gtaactctct tggattatat ggttttggta
     1501 gataatcatc agcacctctt tcaagaccca taactttatc taaaatatca tctcttgcag
     1561 aacttattat tattgggatt tgagattttt ctcgtatttt tggaataagc tccaaaccat
     1621 caatttcagg taaagtcaaa tctaaaatca aaagctgata ctctttgctt aaattcaaca
     1681 tagatagtcc attatatgga ctatctgttg tagttacttc tatatcaaat gattttaaat
     1741 aatccgtaat gatttgagct agctctaaat catcttcaat cataagtatt ttaataataa
     1801 tttatccttt ttattgaatt acaaacataa gcgtttgccc atatctattt atgtaaactc
     1861 gtttatgttg attattatat ctttttaatg ctgtttctat atttgaataa tttttaattt
     1921 caacatcttc tatttgaata ataatatcac ctgctaaaaa cccagatctt tcagcttttg
     1981 attttggttc tacatctgta attaaaattc ccatgctctc aagtggtaat ctatattttt
     2041 ttataatctc agcatttatt ggacttactt ttaatccatt taaaattgta tggtcactat
     2101 ctggttgaac tcctgaaatt atagatctat cagccaaaac tatctcaaca actatatctt
     2161 ttttatctct ttcaatctct agtttaactt tttcatttgg tttaaaagat gcaattgcat
     2221 tttgtaaact atttctatct ttagtctctt taccgtttat tgcgtaaact aaatctcctc
     2281 tttttagacc atattttgct gctggagtgt catttgaaat atctaaaatt atagctccct
     2341 cttttctttt ataaacttgt tgactatctt tgtctaaatc aacaattaca acacccaaat
     2401 accctcttat aacttttcca tcacttacaa gtttaccaac aacatctttt accatttcaa
     2461 caggaattgc aaatccaata ccatcatttc caccactttt tgataaaatt gctgtattta
     2521 ttccaattag tgcacctcta ctatcaacca aagctccacc actatttcca ggatttattg
     2581 aagcatctgt ttgaatataa ttttcatatc tattaatacc tactctattt ttatttagtg
     2641 cagagataat tcccatagtt acagtatttc caactccaaa agggtttcca atagcaaaag
     2701 taacatcacc aaccaataaa gaactagaat cacctaattt aataggtact aaaggcttat
     2761 ttatatcaat ttttacaaca gcaagatcac tatcactatc ttttccaatt agtttagcac
     2821 tatattcagt tggatcatca cctatagtta catttatctc ttcagcattt tcaataacat
     2881 ggtcatttgt tacaatatat ccatcttttg ttacaataac tccagaacc
//
LOCUS       DAOQAN010000078         2922 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_47036_length_2922_cov_4.961981, whole genome shotgun sequence.
ACCESSION   DAOQAN010000078 DAOQAN010000000
VERSION     DAOQAN010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47036_length_2922_cov_4.961981"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(216..>2922)
                     /locus_tag="PKO10_01965"
     CDS             complement(216..>2922)
                     /locus_tag="PKO10_01965"
                     /inference="COORDINATES: protein motif:HMM:NF033527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HNW65954.1"
                     /translation="RYKQLIKSNLFINEYTLDIESQLKHHAIELANNFTNRKKIFFSL
                     VDYSKKINIEVPSYFALSKLIEFALNYQTKSILLHLKTFQKDKRLMLLDDFTNKDENY
                     TNKYNINSFKKLGHSTKKKEMNNSVVQLNTIKSKFDMLKPIIDEVGITDKIAQYHSKW
                     IEQIKMFQLTRKQQLEQQFLLLSFVKYQYFIRNDNLIDRFIAIMQSTKSSLLRHQKDL
                     HFENEPKQRALVQSLEDSNLSILNDVSSIVNNEKLSDRDKIIALRALVETKTQNLKTL
                     LVEKSLIETSNLDRFDFLEKGSQSLQGKLSGVVKLVEFDEKSSNKNLLTAIKYFKESS
                     NIKQDAPLSFLDDEEQQVIFDSEGKIRVSLYKAFLFVHLSDGIRSGILNLKYSYKYKS
                     FEDYLISKDEFISNKKELLKYHELEHLEDFDTFIKPIIQKLEMSFANTNERIEKELNI
                     YFKPTPDSFILTTPKLEKTDHQLEHTIGKYFPQAEFISVIDLLHSVQIKADFLDSFKH
                     YSLQNTPIKKLEPNLLYASIVGYGCNITLSKMAKISKGISENQLDTTTTWYLSEENTM
                     EANDKIVAFIDSLDIAKLLQHDPKYNHTSSDGQKYNMKSSIDSTNAGWSHKYFGLDQG
                     VIVNTFIDEKHRLYYSVVINVSERESGYVIDGLMHNDVVKSDIHSTDTHGFSEVIFGI
                     THLLGFAFGPRIKNFKDQQLYGIFSPKYYLQKGYQLVPKRKINFEIIKEHWDDILRFI
                     LTIKSRKTTASQLLKRLTSYSKHHKLYTAIKEFGKIIKTDFLLTYIDDVELRQRIEKQ
                     LNKIEASNRFSKAVFFGNNAEFIFASQEEQNIANNCKRLIQNSVILWNYLYIDKKLRD
                     AKSQIQKDEIIDAIKNSSIVHWTHINFYGTYDFTKVDKKVSAMIS"
BASE COUNT          908 a          416 c          447 g         1151 t
ORIGIN      
        1 gatttttcca ctaaaagtgt ttttaaattt tgtgttttag tttcaacgag tgctcggcta
       61 cttgggaaca tttgggaaaa atgcgacaaa gaattttaaa ttaaccttaa aatcagatac
      121 tttgctctat gagcctctag aattgatttt cccatacaaa gatacccttt caactccaaa
      181 aacgatttct agctcaattt cataaatatt ctattctaac tgatcattgc actcactttt
      241 ttatcaactt ttgtaaagtc atatgtgcca taaaaattga tatgagtcca gtgaactatg
      301 gaactatttt tgattgcatc aatgatttca tctttttgaa tctgagattt agcatctctt
      361 agttttttat caatatagag gtaattccaa agtatcacag aattttggat aagtcgtttg
      421 caattatttg cgatattttg ctcctcttga gaagcgaata tgaactctgc attatttcca
      481 aaaaatactg cttttgaaaa acgatttgaa gcttctattt tattgagctg tttttcaatc
      541 ctttgacgaa gctccacatc atcgatatag gtaaggagga aatcggtttt gataattttt
      601 ccaaactctt taattgcagt gtagagttta tggtgttttg agtaagatgt aagtcgcttg
      661 agtaattgtg aagctgttgt ttttcttgat tttatcgtaa ggataaatct taaaatatca
      721 tcccaatgtt ctttgataat ctcaaagttg atttttcgtt ttggaactaa ttgatatcct
      781 ttttgtaaat agtattttgg tgaaaagatt ccatagagtt gttgatcttt aaagttttta
      841 atccttggtc caaatgcaaa tcctaacaaa tgagttattc caaatataac ttctgaaaaa
      901 ccatgtgtat cggtgctgtg gatatcactt ttaacaacat cattatgcat aagtccatca
      961 attacatatc cactttccct ttcacttaca ttgataacaa cagagtaata caatcgatgt
     1021 ttttcatcta taaaagtgtt aacaataaca ccttgatcta atccaaaata tttatgagac
     1081 catccagcat tagttgaatc gattgatgat ttcatattat acttttgccc atcacttgat
     1141 gtatgattat acttaggatc atgctgtagt aatttcgcaa tatcaagtga atcgataaat
     1201 gcaacaattt tatcatttgc ttccatggta ttttcttcac ttaagtacca tgtagtggta
     1261 gtatcaagtt gattctcact gatcccttta gatatttttg ccatctttga aagtgtgata
     1321 ttacaaccat atccaacaat tgaagcatat aacagatttg gttcaagttt tttaataggt
     1381 gtattttgga gtgaatagtg tttaaatgaa tctaaaaaat ctgcttttat ttgcaccgaa
     1441 tggagcagat caattacaga tataaactct gcttgtggaa aatattttcc aatagtgtgt
     1501 tctaattgat gatctgtttt ttctaatttt ggagtagtga gaataaacga atctggtgta
     1561 ggtttaaaat agatattgag ctctttttca attcgttcat tggtgtttgc aaagctcatt
     1621 tcaagttttt ggataattgg ttttataaaa gtatcgaaat cttcaagatg ttccaattca
     1681 tgatatttga gtagctcttt tttattgctt ataaactcat ctttagagat taaataatct
     1741 tcaaagcttt tgtatttata ggagtatttg agatttaaaa tccctgatcg tatcccatca
     1801 ctaagatgaa caaataaaaa tgctttatag agtgagaccc ttattttccc ttcactatca
     1861 aatattacct gctgttcttc atcatctaaa aaactcaatg gtgcatcttg tttgatatta
     1921 gatgattctt tgaaatattt gatagcagtt agtagatttt tattagatga tttctcatca
     1981 aactctacta atttaactac tccagataat tttccttgaa gtgattgtga tcctttttca
     2041 agaaaatcaa atcgatctaa atttgaagtt tcaattagag atttttccac taaaagtgtt
     2101 tttaaatttt gtgttttagt ttcaacgagt gctcttaggg caatgatttt atctctgtca
     2161 ctaagctttt cattgttaac aatactactc acatcattta aaattgaaag atttgaatcc
     2221 tctaaagatt gcactaaagc cctttgttta ggttcatttt caaagtgaag atccttttga
     2281 tggcgaagaa gagagctttt agtactttgc ataatagcaa taaatctatc gattaggtta
     2341 tcatttctga taaaatattg atattttaca aacgaaagaa gtaaaaattg ttgctctaac
     2401 tgttgttttc ttgtaagttg aaacatttta atttgctcaa tccattttga gtgatactgt
     2461 gcaattttat ctgtaatacc aacttcatca ataattggtt tgagcatatc aaatttggat
     2521 ttgatggtgt ttagttgtac tactgaatta ttcatctcct ttttcttagt agagtgtcct
     2581 aattttttga agctattgat gttatatttg tttgtataat tttcatcttt attggtaaaa
     2641 tcatcaagaa gcattagtct tttatctttt tgaaaagttt ttagatgaag cagaatactt
     2701 tttgtttgat aattgagtgc aaactcaata agttttgaca aggcaaaata gctaggtact
     2761 tcaatgttta tcttttttga gtaatctaca agtgaaaaaa agattttttt acgattagta
     2821 aaattattcg ctagttcaat ggcatggtgt tttagttgtg attcaatatc taaagtatat
     2881 tcattgataa agagatttga tttgataagt tgtttatatc gt
//
LOCUS       DAOQAN010000079         2900 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_47590_length_2900_cov_3.072056, whole genome shotgun sequence.
ACCESSION   DAOQAN010000079 DAOQAN010000000
VERSION     DAOQAN010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47590_length_2900_cov_3.072056"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..767
                     /locus_tag="PKO10_01970"
     CDS             <1..767
                     /locus_tag="PKO10_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014475065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HNW65955.1"
                     /translation="DANPYGMAGTRIHDHSHPDWRQDVDILVNGAGEKLAYITLPKST
                     CYEDAKTQIEYIQAVAKKAGIKREIPIHVLIETHGALQDVEKIATLPWLQVLDFGLMD
                     FVSGYQGAIPAINMRSPGQFDHRLIGAAKARVAQAAIQNCVIPCHNVTLDLKNPYQTY
                     KDAERARNEFGFMRMWSIYPTQVQAIVDAMKPDFTELEAAQNILIKAQDAEWGPIQYD
                     GELHDRATYRYFWELVQRAKYSGVKLMEEAEKRFFA"
     gene            962..2461
                     /locus_tag="PKO10_01975"
     CDS             962..2461
                     /locus_tag="PKO10_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014475066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HNW65956.1"
                     /translation="MSKKITEQDIIDSVASACQFISFYHPEDFVKGMVEAYENEKSDA
                     AKNAIGQILINSKMCAMGHRPLCQDTGSVNIFVKVGLKANLDITKNLEDLLNEGVAKG
                     YTDPDNTLRYSVVADPAGKRTNTKNNTPAVIHVSVDNSDKLDITVAAKGGGSENKSKF
                     TVLNPSDSIYDWVMSNVKDMGAGWCPPGILGIGIGGNPEKAMLLAKESLMGHVDIHEL
                     KARGAQTPLEELRLKLYEDINKLGIGAQGLGGLTTVLDVKILDYPCHAASLPVAMIPN
                     CAATRHIHFELDGNGPAKFEKPDLDLWPDIKLPMDTIKRVNIEDLTKENLSQFKSGDT
                     LLLSGKILTARDAAHKKIVEYKNAGKPLPNGVDLKDKFIYYVGPVDPVKGEVVGPAGP
                     TTSTRMDKFTKDMMEIGIMGMIGKGERKQPTIDLIKEYKSIYLIATGGAAYLIAQSIK
                     GAKTLAFEELGMEAIYEFEVKDMPVTVAVDTEGNSIHTTGPAKWRTINK"
     gene            2615..>2900
                     /locus_tag="PKO10_01980"
     CDS             2615..>2900
                     /locus_tag="PKO10_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014469263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5718 family protein"
                     /protein_id="HNW65957.1"
                     /translation="MNLIEDLKDYLGFAVAGNFANHLGEAGEADEFSVIETKEKDEPK
                     GMFPFYIKGHDSFLGTYPICDEVILTHGREDDKLQVEAEVALICDFVYENE"
BASE COUNT         1091 a          373 c          523 g          913 t
ORIGIN      
        1 ctgatgcaaa cccttatggt atggcaggaa caagaatcca tgaccattct catccagatt
       61 ggagacaaga tgttgatatt ttagtaaatg gtgctggtga aaaattagca tatattactt
      121 taccaaaatc aacttgctat gaagatgcta aaactcaaat tgagtatatt caagctgttg
      181 caaaaaaagc tggaattaaa agagagattc caattcacgt acttatagag actcatggag
      241 ctctacaaga tgttgaaaaa attgcaactt taccttggtt acaagtttta gattttggtc
      301 taatggattt tgtaagtgga tatcaaggag caattccagc aattaatatg agaagtccag
      361 gtcaatttga tcacagatta attggagctg caaaagcaag agttgcacaa gctgctattc
      421 aaaactgcgt aattccttgt cacaatgtaa ctttagattt aaaaaatcca taccaaacat
      481 acaaagatgc agaaagagct agaaatgagt ttggatttat gagaatgtgg tcaatttacc
      541 caacacaagt tcaagctatt gttgatgcta tgaaacctga ttttacagaa cttgaagcag
      601 cacaaaatat tttaattaaa gctcaagatg ctgagtgggg accaattcaa tatgatggtg
      661 agttacacga tagagcaact tatagatact tttgggaatt agttcaaaga gctaaatatt
      721 ctggtgttaa attaatggaa gaagcagaaa aaagattttt tgcctaattt tcagatgatt
      781 taaaaagcta ctctttttta agggtagctt tttttaattc ttaaattttt gatatatttc
      841 aaaactaaac aatttttaat atttttaaaa atttccatat aaaatatgtt ttgtaaaagc
      901 caattttata tggtgcattg atataattaa aagatttcta aaaccaaaaa aggaaaaatt
      961 aatgagtaaa aaaatcacag aacaagatat tattgattca gttgcaagtg cctgtcaatt
     1021 tatatctttt tatcatccag aagactttgt aaaaggtatg gttgaagctt atgaaaatga
     1081 aaaaagtgat gcagcaaaaa atgcaattgg acaaatttta attaactcaa aaatgtgtgc
     1141 tatgggacac agacctctat gtcaagatac aggaagtgtt aatatttttg taaaagttgg
     1201 tttaaaagca aacctagata ttacaaaaaa tttagaagat ttattaaatg aaggtgttgc
     1261 aaaaggttat acagatcctg ataatacttt aagatactca gttgtagctg atcctgctgg
     1321 aaaaagaaca aatacaaaaa acaatactcc agcagtaatt catgtaagtg ttgataactc
     1381 tgataaactt gatattacag ttgcagcaaa aggtggagga agtgaaaaca aatctaaatt
     1441 tactgtttta aatccaagtg atagtattta tgattgggtt atgtcaaatg taaaagatat
     1501 gggtgctgga tggtgtcctc ctggaatttt aggaattgga attggtggaa atcctgaaaa
     1561 agcaatgcta cttgcaaaag agtctttaat gggacatgtt gatattcatg aactaaaagc
     1621 aagaggcgct caaactccac ttgaagagtt aagacttaaa ctttatgaag atataaataa
     1681 actaggaatt ggagctcaag gtcttggtgg attaacaact gttttagatg ttaaaatttt
     1741 agattatcca tgtcatgctg cttctttacc agttgctatg attccaaact gtgcagcaac
     1801 aagacatatt cactttgaat tagatggaaa tggacctgct aaatttgaaa aacctgattt
     1861 agatctttgg ccagatatta aacttccaat ggatactatt aaaagagtaa atattgaaga
     1921 tttaacaaaa gagaatttat ctcaatttaa atctggtgat acacttttat tatctggaaa
     1981 aattttaaca gcacgtgatg ctgctcataa aaaaatagtt gaatacaaaa atgctggaaa
     2041 acctcttcca aatggtgttg atttaaaaga taaattcatc tactacgttg gaccagttga
     2101 tccagtaaaa ggtgaagttg taggaccagc aggaccaaca acatctacaa gaatggataa
     2161 atttacaaaa gatatgatgg aaattggaat tatgggaatg attggaaaag gtgagagaaa
     2221 acaaccaaca atcgatttaa taaaagagta caaatctatc tatcttatag caactggagg
     2281 agctgcttat ttaattgctc aatcaattaa gggtgcaaaa actttagcat ttgaagagct
     2341 tggaatggaa gctatttatg aatttgaagt aaaagatatg cctgtaactg ttgctgttga
     2401 tacagaagga aactctattc atacaacagg acctgctaaa tggagaacta ttaataaata
     2461 gttcttattt tacaaagagc cacaaatact ataggctctt tgtaaatatt ttcttaaact
     2521 actcttaaat taacaaatta tatttcgttt ttgactttta aaccaatttt tttcgataac
     2581 attgcagttc aaaatatttt aggattttat aattatgaat ttaatagaag atttaaaaga
     2641 ttatttaggt tttgcagttg ctggaaactt tgcaaatcat ttaggagaag ctggagaagc
     2701 tgatgagttt tctgtaattg aaacaaaaga gaaagatgaa ccaaaaggta tgtttccatt
     2761 ttatataaaa ggtcatgata gttttttagg aacttaccct atttgtgatg aagtcatatt
     2821 aactcatgga agagaagatg ataaattaca agttgaagca gaggttgctt taatttgtga
     2881 tttcgtatat gaaaatgaaa
//
LOCUS       DAOQAN010000080         2870 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_48402_length_2870_cov_2.889520, whole genome shotgun sequence.
ACCESSION   DAOQAN010000080 DAOQAN010000000
VERSION     DAOQAN010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48402_length_2870_cov_2.889520"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /gene="tmk"
                     /locus_tag="PKO10_01985"
     CDS             complement(<1..483)
                     /gene="tmk"
                     /locus_tag="PKO10_01985"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HNW65958.1"
                     /translation="MYVVIEGIDTAGKSTQLEILKNKFPNAIFTKEPGGTALGIKLRE
                     MILSGEANNSLAEMFMFLADRAEHTQEIILKNKNKLIISDRSFISGIAYAKDRKIDDL
                     IELNRLATSNNFPQKVILLELKKDELQKRLSQKPNDSIEKRGLDYLLDIQARLKETIL
                     K"
     gene            complement(474..968)
                     /gene="coaD"
                     /locus_tag="PKO10_01990"
     CDS             complement(474..968)
                     /gene="coaD"
                     /locus_tag="PKO10_01990"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HNW65959.1"
                     /translation="MKNNDYNYTGSYKKAIYSGTFDPITIGHLDIIERAIKVFDEVII
                     SVAKSEMKKPMFSHNKRVEFVEASTKHLKNVRVVGFDTLLVDLARDLQINTIIRGLRA
                     VSDFEFELQMGYANSSINHNLETVYLMPTLEHAFVSSTIVREIIRFEGRFEHLVPKEV
                     IKCM"
     gene            complement(971..1525)
                     /locus_tag="PKO10_01995"
     CDS             complement(971..1525)
                     /locus_tag="PKO10_01995"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HNW65960.1"
                     /translation="MKLTVGISGASGVNLALKFIKQIPKDIEIFVVFSKSSKKALKLE
                     NGIKTKEIFKDYENITLFKDKNIGASLASGSFKVDKMIILPCSSNTLAKCSVGIADSL
                     ITRAFSVMLKEKREIIIAPRELPLNTIMLENMLKLSNLGVVIAPPILGYYSSQQTLED
                     MENFIIGKWMDLLKIDNKLYKRWK"
     gene            1690..2319
                     /locus_tag="PKO10_02000"
     CDS             1690..2319
                     /locus_tag="PKO10_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014473933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HNW65961.1"
                     /translation="MSKNFTYIRKNRNNMSYTDEQKELISKYILKNSLTIYKHIEIEI
                     NRPSQEKNIIQLLENCEKNSTIIVANLNVFGRTIDRILEIVKFLLSNKIRIIVVEQNL
                     DLLSNEDKLTLMILNIIKMTISLEKELQSLRTKEALNAKKEDGIPLGKPKGTIQKSKF
                     DEQRDKIEELLGMGLSVRKIAKLLGYNNHIGLNSYVKKRDIRAIALDKK"
     gene            2392..2838
                     /gene="rplI"
                     /locus_tag="PKO10_02005"
     CDS             2392..2838
                     /gene="rplI"
                     /locus_tag="PKO10_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HNW65962.1"
                     /translation="MKVLLIKDVKTLGKAGEIKEVADGYGKNFLIGKGLALQATNEVI
                     AKHNAEQKKLALKEEEEIKAAKELAEKINSTKLTIRHKVGANGQLIGSVTNKEISEEL
                     EKQFSIMVDKKSIVVDNKLKTIGIYEVSCKLGHSVNATLKIDIIAG"
BASE COUNT         1091 a          382 c          368 g         1029 t
ORIGIN      
        1 ttttaaaata gtctctttta atctcgcttg aatatccaaa agataatcta atcctctttt
       61 ttcaatacta tcatttggtt tttgagacaa ccttttttgt aattcatctt tttttagttc
      121 tagtaaaatt actttttgtg gaaaattatt tgaagttgct aaacgattta attctattaa
      181 atcatctatt tttctatctt ttgcataagc aattccagat ataaacgaac ggtcactaat
      241 aattaattta tttttatttt ttaatattat ctcttgagtg tgttcggctc tatctgcaag
      301 aaacataaac atttcagcta aactattatt tgcttctcca cttaaaatca tctctcttag
      361 ctttatacct agagcagttc cacctggttc ttttgtaaaa attgcatttg gaaatttatt
      421 ttttaaaatt tctaattgag ttgatttccc tgctgtatca attccttcaa taactacata
      481 cattttataa cctctttagg aactaaatgt tcaaatctac cttcaaatct tataatttct
      541 cgcacaattg tagagcttac aaatgcatgt tctagtgttg gcattaggta aactgtttca
      601 agattatggt tgattgaaga gtttgcatac cccatttgta gttcaaattc aaaatcacta
      661 acagctctta atcctctgat tattgtatta atttgtaaat ctcttgccaa atcaactaaa
      721 agagtatcaa atcctacaac tcttacattt ttaagatgct tcgtagatgc ttctacaaac
      781 tctactctct tattatgaga aaacataggt tttttcattt cactttttgc aacacttata
      841 attacttcat caaaaacttt tattgctctt tcaataatgt ctaaatgtcc aatagtaatt
      901 gggtcaaaag tcccactata aatagctttt ttatatgaac cagtatagtt ataatcgtta
      961 tttttcatat ctatttccat cttttatata gtttgttatc tatttttaat agatccatcc
     1021 actttccaat aataaaattt tccatatctt ctaaagtttg ttgagaacta taatatccca
     1081 aaattggagg tgcaataaca acacctaaat ttgaaagttt tagcatattt tctaacatta
     1141 ttgtatttaa aggtaactct cttggagcta ttataatctc tcttttctct tttagcatta
     1201 cagaaaaagc cctagtaatt aagctatcag caattcccac actacatttt gccaaagtat
     1261 ttgaactgca aggaaggata atcattttat caactttaaa tgatccagaa gctaaagaag
     1321 cacctatatt tttatcctta aaaagtgtta tattttcata atctttaaaa atttctttcg
     1381 tttttatacc attttctagt tttaaagcct tttttgaact ttttgaaaat acgacaaaaa
     1441 tctcaatatc ttttggtatc tgttttataa atttcaaagc tagatttact ccactagcac
     1501 ctgaaattcc aactgttaat ttcaatattt acctcttgaa taatttaatt ataaaaactt
     1561 ttatactatc aaataaaagc ttcaagtttt attttatata aaaatatgta taagctttta
     1621 ttaaatatta aaaagataac attttgtata aacttttata cataaaacta ttacaaatga
     1681 ggtgtaaaaa tgtcaaaaaa tttcacatat attagaaaaa atcgaaataa tatgagttat
     1741 acggatgaac aaaaagagct tatttctaaa tatattttaa aaaattcatt aactatttac
     1801 aaacatattg aaatagagat aaatagacct tctcaagaga aaaatattat tcaactttta
     1861 gaaaattgtg aaaaaaactc tacaataatt gttgcaaact taaatgtttt tggaagaact
     1921 attgatagga ttttagagat tgtaaagttt ttgttatcaa ataaaataag aataattgtt
     1981 gttgaacaaa atttagattt attaagtaac gaggataagc tcacattgat gattttaaat
     2041 attataaaaa tgactatatc tttagaaaaa gagcttcaaa gtttgagaac aaaagaggca
     2101 ttaaatgcaa aaaaagagga tggtattcct cttggaaaac caaaaggtac tattcaaaaa
     2161 tcaaaatttg atgagcaaag ggataaaata gaagagcttc ttggtatggg acttagtgtt
     2221 agaaaaatag caaaactttt aggatataac aaccatatag gtttaaatag ttatgtgaaa
     2281 aagagagata ttagggcaat tgcgcttgat aaaaagtagc tttaatactt tttatatggc
     2341 tttaaaaaaa ttaatagtag aatgatgagt taaaaattaa aggaaaagta aatgaaagta
     2401 ttattaataa aagatgtaaa aactttagga aaagcaggtg aaatcaaaga agtagctgat
     2461 ggatatggta aaaatttttt aataggaaaa ggcttagctt tacaagcaac aaatgaagtt
     2521 atagcaaaac ataatgcaga acaaaaaaag ctagcactta aagaagaaga agagataaaa
     2581 gctgcaaaag agttagcaga gaaaataaat tcaacaaaac ttacaattag acataaagtt
     2641 ggagcaaatg gacaacttat tggaagtgtt acaaataaag agataagtga agaacttgaa
     2701 aaacagttct ctattatggt tgataaaaaa agtatagttg ttgataataa attaaaaact
     2761 attggtattt acgaagtttc ttgtaaatta ggacactcgg taaatgcaac gttaaaaata
     2821 gatataatcg ctggttaatt atcattcaga aaactacaat tttagcctat
//
LOCUS       DAOQAN010000081         2829 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_49496_length_2829_cov_2.239366, whole genome shotgun sequence.
ACCESSION   DAOQAN010000081 DAOQAN010000000
VERSION     DAOQAN010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49496_length_2829_cov_2.239366"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..895)
                     /locus_tag="PKO10_02010"
     CDS             complement(53..895)
                     /locus_tag="PKO10_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HNW65963.1"
                     /translation="MKKTELKQYIFIILGSFAMAFGTVCFLSPNEIITGGGVGISLLL
                     HAIFPQITLGIIIAVVSIPFLILSYIYFGKYYLFKTFIVVLLLSTFTDILKEVLKIEA
                     ITHDILLAAVFGGIFIGLGVGLVIKGRASTGSTSVVGEIVAKKTKYKAAEVLLAIDAT
                     IMFASVFVYNDIDKSLYSMLSVYVGIRVLDIILTGRPSKKIVNIVSNNVEVLKEQIRE
                     RIEEHGTILTGIGLHQGQNKTIIYVTVDAGKIDLLKNLITKYDPDAFMIITEASEFLG
                     RGLK"
     gene            complement(903..2141)
                     /locus_tag="PKO10_02015"
     CDS             complement(903..2141)
                     /locus_tag="PKO10_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014474524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNW65964.1"
                     /translation="MNKIYDLIVVGAGPAGIMASISAAKDGKKVLIIEKMTQIALKLK
                     ATGGGKCNLTNTLSQDDFCTKFGKSGRFIKDALDSFSRDDLLAFFASIGVATIAKDGF
                     RVFPVNHSSTIILEALQNELERLKVEVKTSINIKSIKKEKDIFEIKTDEDIFKSKNLV
                     LATGGLGYPTLGATGDGYIFASSFGHTITQTSPAMMPLFTKEKNFASCKADTIAKAIL
                     KVNIPKYKNLKLTGDLIFTKEGLRGPVILDFAREITPILEKYDEVPLLINFLKGKNEE
                     DIFQHLKNEIAKNPQNTILQNLETLLASSVSKEILNICEIDENLRFKQIEGLKREKLI
                     KTLAWTPFTIVGHDGFRQAMITRGGVSLKEIEQKTMQSKILQGLYFCGELVDIDGPCG
                     GYNLQWSFSSGFLAGKLNKN"
     gene            2213..>2829
                     /locus_tag="PKO10_02020"
     CDS             2213..>2829
                     /locus_tag="PKO10_02020"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNW65965.1"
                     /translation="MLGRVLLVFTILSLYSFATTNNKIALLTFDDGPIKATKNIIETV
                     REENIPVTMFFIGCQIENFPNIYKDAINYPNILIGNHTYSHANNRYRKFYSDPLLVVE
                     DIKKANSIVGVDNISNTSSAFLPVRLAGRNVFRLPTITKNDNMIDTIQREKEIVGYDN
                     IYNEGYYIYGWDLEWAYEKNGKPILTPQEIYNSMEKIYEKKLSSK"
BASE COUNT          989 a          464 c          330 g         1046 t
ORIGIN      
        1 tagtatattt ttttttattt gcacctaaat caaattttgt aacaaaacta aactacttta
       61 aacctctacc caaaaactct gaagcctctg ttattatcat aaaagcatct ggatcatatt
      121 tagtgattaa attttttaat aaatcgatct ttcccgcatc aactgtaaca taaatgatag
      181 ttttattttg cccttgatga agccctatgc ctgttaaaat agtaccgtgt tcctctattc
      241 tttctcttat ttgctctttt aaaacttcaa cattgtttga tacgatattt actatctttt
      301 ttgatggtcg tcctgttaga ataatatcaa gtactctaat tccaacataa acactaagca
      361 tactataaag tgatttatct atatcattat aaacaaaaac agaagcaaac ataattgttg
      421 catcaatagc taataaaact tctgctgctt tatattttgt cttttttgca actatttctc
      481 ctacaacaga tgtacttcca gtagatgctc ttccttttat aactaatcca acacctaaac
      541 ctataaaaat tccaccaaaa actgcagcta gcaaaatatc atgcgtaatt gcttctattt
      601 ttaaaacttc ttttaatata tctgtaaatg ttgataaaag aagaactaca ataaatgttt
      661 taaaaagata atattttcca aaatagatat aagataaaat caaaaatggg atacttacaa
      721 ctgcaataat aattcccaaa gtaatttgtg gaaaaattgc atgtaaaagt aaagagatac
      781 caactccacc acctgtaatt atctcattgg gagataaaaa acagactgtt ccaaatgcca
      841 ttgcaaatga gcctaaaatt ataaatatat attgttttaa ttctgttttt ttcatatttt
      901 tcctaatttt tattcaattt tccagctaaa aaaccacttg aaaatgacca ttgaagattg
      961 tatccaccac aaggtccatc aatatctaca agttctccgc aaaaatataa accttgtaaa
     1021 attttacttt gcatagtttt ttgttctatc tctttcaaag agactccacc tcttgtaatc
     1081 atagcttgtc taaatccatc atgacctaca atagtaaaag gtgtccaagc tagagttttt
     1141 attagttttt ctctttttaa cccttctatt tgtttaaatc ttaaattctc atcaatttca
     1201 caaatattta aaatctcttt agaaacacta cttgcaagaa gtgtttctaa attttgtaaa
     1261 atagtgtttt gtggattttt agcaatttca ttttttaaat gctgaaaaat atcctcttca
     1321 ttcttacctt ttaaaaaatt tatcaaaagt ggaacttcat catatttttc taaaattgga
     1381 gtaatttctc tagcaaaatc caaaataaca ggacctctta acccctcttt tgtaaagatt
     1441 aaatctcctg ttagttttaa atttttatat tttggaatat ttacttttaa tatagctttt
     1501 gcaattgtat cagccttaca agaagcaaaa tttttctctt ttgtaaaaag tggcatcata
     1561 gcaggagagg tttgtgtgat tgtatgacca aaagatgaag caaaaatata tccatctcct
     1621 gttgctccta aagttggata acctaaacct cctgttgcca aaactagatt tttactttta
     1681 aaaatatctt catctgtttt gatttcaaaa atatctttct ctttttttat actttttata
     1741 tttatcgaag ttttaacttc aacttttaat ctctcaagct cattttgcaa tgcttctaaa
     1801 ataatagtag agctatgatt tactggaaat actctaaatc catctttagc aattgtagca
     1861 actccaattg aagcaaaaaa agcaagcaaa tcatctcttg aaaaactatc taatgcatct
     1921 tttatgaatc tgccactctt accaaattta gtacaaaaat catcttgaga taaagtattt
     1981 gtaagattac actttcctcc gcctgttgct tttagtttca gtgcaatttg tgtcattttt
     2041 tcaattatta atactttttt tccatctttt gcagcactta tacttgccat tattccagca
     2101 ggtcctgccc caacaactat taaatcataa attttgttca atttttctct tcttttattg
     2161 agtttcttgt attatacaca atttaattta agcatttagg ggttgtgtgt ctatgcttgg
     2221 tcgagtttta ttagttttta caattttatc actatactca tttgcaacta caaacaataa
     2281 aatagcactt ttgacttttg atgatggtcc aataaaagca acaaaaaata tcatagaaac
     2341 tgtaagagaa gagaatattc ctgttactat gttttttatt ggttgtcaaa ttgaaaattt
     2401 cccaaatata tacaaagatg ctataaatta tccaaatatt ttaataggaa accatacata
     2461 ctctcacgca aataatagat atagaaagtt ttatagtgat cctcttttag ttgtagaaga
     2521 tataaaaaaa gcaaattcaa tagtaggagt tgataatatt tcaaatactt catctgcttt
     2581 tttaccagta agacttgctg gaagaaatgt ttttagactt ccaacaatta ctaaaaatga
     2641 caatatgatt gatacaatac aaagagaaaa agagattgtt ggttatgata atatctataa
     2701 tgagggctat tatatttatg gttgggattt agaatgggct tatgaaaaaa atgggaaacc
     2761 aatactaact cctcaagaaa tatataactc tatggaaaaa atatatgaaa aaaaactctc
     2821 aagcaaaga
//
LOCUS       DAOQAN010000082         2809 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_50048_length_2809_cov_3.008351, whole genome shotgun sequence.
ACCESSION   DAOQAN010000082 DAOQAN010000000
VERSION     DAOQAN010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2809
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50048_length_2809_cov_3.008351"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="PKO10_02025"
     CDS             complement(<1..371)
                     /locus_tag="PKO10_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014475432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein]--UDP-N-
                     acetylglucosamine O-acyltransferase"
                     /protein_id="HNW65966.1"
                     /translation="MSNIHKTAIIEDGAILGENVTVGAYTIIGKDVKIGDGTVIDSHT
                     VIAGKTTIGKNNHIFSHASIGTIPQDLKFNPNDDVELIIGDNNKIREYTLFNPGTVTG
                     HAKTVIGNNNLFMGYVHVAHD"
     gene            complement(384..827)
                     /gene="fabZ"
                     /locus_tag="PKO10_02030"
     CDS             complement(384..827)
                     /gene="fabZ"
                     /locus_tag="PKO10_02030"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009649448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HNW65967.1"
                     /translation="MFDIIQIQEILPHRYPLLLVDRITDMEVAKSIKGFKNISISEPA
                     FQGHFPGHPIYPGVMILEGMAQCGGVLALKSSGLTDEQMKDKVIYFMSIDKAKFRNPV
                     RPGDRLDYELTAIKMKSTLMVLEGKAYVDGKLTAEAEFKAMIVDK"
     gene            complement(881..1924)
                     /gene="lpxB"
                     /locus_tag="PKO10_02035"
     CDS             complement(881..1924)
                     /gene="lpxB"
                     /locus_tag="PKO10_02035"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014469478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HNW65968.1"
                     /translation="MKVLVSAMETSSNIHLKELKKYLDDDIEFVGVFDKELGNPLYDL
                     SSLAIMGFVDALKKLRFFFRLRDELVELAKDCDKVLLLDSSGFNLPLAKKLRETYPNK
                     EIIYYILPQAWAWKRKRVIALEKYCTKLCSILPFESDIYNDKSKITYVGHPLLDEIPK
                     FKNELSNSGKIVFMPGSRKTEIKNLMPVFKDLIKKIPNKDYILVIPSKFSDEYIKKVY
                     GDIGDFKISKNAHEALLEAEYAFICSGTATLEAALIGTPFTLSYIAKKLDFFIGKTFV
                     KLNYVGLANIFFEKMTNKKLHSEFLQNEVSVENLLKDYKNLDRELFFENSKLLRVYLK
                     HGSSRNVASIIKN"
     gene            complement(1921..>2809)
                     /locus_tag="PKO10_02040"
     CDS             complement(1921..>2809)
                     /locus_tag="PKO10_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014475429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HNW65969.1"
                     /translation="GAYNLEEWLKKVKGMEHLPEKGDRCTVCYDDRLDNSVQKAIELG
                     HDKFTTTLLISPKKSQEKLEIIGNNLFKKTGVEFIFRDYRSGNGAEIQGVRVKENSLY
                     RQNYCGCLFGLTAQREHQKKIMDEMFNPISKQILPESIEQRLELYKKRNTLEDEKKEY
                     KLIKQRFLNYRLLGGKVVVNKEVIPSYIFAYSTINKNQTSSKIEYEKDGINYLAKDEV
                     KIISLNTFNKFAQTKYKNTKELMFKSINFEDEIKLRADILKNPFDLSALIVIDEIIDS
                     KYEIFINSVTYEDIKEEIV"
BASE COUNT          927 a          480 c          299 g         1103 t
ORIGIN      
        1 caatcatgag ccacatgaac atatcccata aataaattat tattcccaat aactgtttta
       61 gcatgtcctg ttacagttcc tggattaaat aaagtgtatt ctctaatctt attattatca
      121 ccaataatta attcaacatc atcatttgga ttaaacttta aatcttgtgg aattgttcca
      181 attgaagcat gtgaaaaaat atgattattt ttaccaatag ttgtttttcc agctattact
      241 gtatgagagt cgattacagt tccatcaccg atttttacat cttttcctat aatagtatat
      301 gcgccaaccg ttacattttc ccctaaaatt gcaccatctt caataatggc tgttttgtga
      361 atattactca ttttcgacct taattattta tcaactatca ttgctttaaa ctctgcttca
      421 gcagttagtt taccatcaac ataagccttt ccttcaagta ccattaaagt acttttcatt
      481 tttatagctg ttaattcata gtcaagtcta tctcctggac gaactggatt tctaaatttt
      541 gccttgtcta tactcataaa atatataact ttatctttca tctgctcatc agttaaacct
      601 gaactcttaa gagctaaaac tccaccacat tgagccattc cttcaagaat cataacacct
      661 ggataaattg gatgcccagg aaaatgtcct tgaaatgcag gttcagatat agagatattt
      721 ttaaaacctt ttatagattt tgcaacttcc atatctgtaa ttctatctac tagaagaaga
      781 gggtatctgt gaggtaaaat ctcttgaatt tgtataatat caaacataat aattcctgaa
      841 tttaaaattg ctaaagttgt ggattataac taaaagtagt ttagtttttt ataatgcttg
      901 caacatttct tgagcttcca tgttttaaat agactcttaa aagctttgaa ttttcaaaaa
      961 aaagttctct atctaaattt ttataatctt ttaaaagatt ttcaacactt acttcatttt
     1021 gtaaaaattc actatggagt ttcttatttg tcattttttc aaaaaagata tttgcaagcc
     1081 ctacataatt taattttaca aaagtttttc caataaaaaa atctaatttt tttgctatat
     1141 aagataaagt aaaaggagtt cctataagtg cagcttctaa agttgcagtt ccagagcaaa
     1201 taaaagcata ttcagcttct aaaagtgctt cgtgtgcatt tttagatatt ttaaaatcgc
     1261 caatatctcc ataaactttt tttatatatt catcactaaa ttttgatgga attaccaaaa
     1321 tataatcttt attcgggatt ttttttatca aatctttaaa aacaggcatt aagtttttta
     1381 tctcagtttt tctactgcct ggcataaaaa ctatttttcc tgaatttgat agctcatttt
     1441 taaattttgg tatctcatct aaaagaggat gtcctacata agttatttta gatttatcat
     1501 tataaatatc actttcaaaa ggtaaaattg aacataattt tgtacaatat ttctctaaag
     1561 caattactct ttttctcttc caagcccaag cttgaggaag aatataataa ataatctctt
     1621 tatttggata tgtttctctt agtttctttg ctaaaggcag attaaatcca ctactatcaa
     1681 gaagtaaaac cttatcacaa tcttttgcta actctacaag ttcatctcta agacgaaaaa
     1741 agaatcttaa ttttttgaga gcatcaacaa atcccataat tgctaaagag cttagatcat
     1801 aaagtggatt tcctaactcc ttatcaaaaa ctccaacaaa ctcaatatca tcatctaaat
     1861 atttttttaa ctcttttaga tgaatattcg atgatgtctc catagcactt actaaaactt
     1921 tcatacaatc tcctctttta tatcttcata tgttacacta tttataaaaa tttcatattt
     1981 agaatcaata atctcatcaa ttacaattaa agcacttaaa tcaaaaggat tttttaaaat
     2041 atctgctctt aattttatct catcttcaaa atttatagat ttaaacatca attctttcgt
     2101 atttttatac ttagtttgtg caaatttgtt aaaagtattc aaagagatta tttttacttc
     2161 atcttttgcc aaatagttta ttccatcttt ttcatactct atttttgaac tagtttgatt
     2221 tttatttata gtagagtaag caaaaatata agagggaata acctctttat ttacaacaac
     2281 ttttcctcct agtaacctat aatttaaaaa tctttgttta attaatttat actctttttt
     2341 ctcatcttca agagtgtttc tttttttgta aagttctaat ctttgctcta ttgattcagg
     2401 taaaatttgt ttagaaatag gattaaacat ctcatccata atcttttttt gatgttctct
     2461 ttgagccgtt aaaccaaata aacaaccaca ataattttgt ctataaagac tattctcttt
     2521 tacacgaact ccttgaattt ctgcaccatt accacttctg taatctctaa aaataaactc
     2581 tactccagtt tttttaaaaa gattatttcc aataatctca agtttttctt gggatttttt
     2641 tggagaaatt aataaagttg tagtgaactt atcatgaccc aattctattg ctttttgaac
     2701 actattatca agcctatcgt catagcaaac tgtacatcta tctccttttt caggtaaatg
     2761 ctccattcct ttgacttttt ttaaccactc ttcaaggtta taagcacct
//
LOCUS       DAOQAN010000083         2801 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_50291_length_2801_cov_2.394392, whole genome shotgun sequence.
ACCESSION   DAOQAN010000083 DAOQAN010000000
VERSION     DAOQAN010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50291_length_2801_cov_2.394392"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1195
                     /locus_tag="PKO10_02045"
     CDS             <1..1195
                     /locus_tag="PKO10_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNW65970.1"
                     /translation="ALDGTIKDTSHIFAIVLSLAKKGELKRVVSSLESAKKLGNIPNI
                     DIYVVNTLNSAIEFVKSKDKDNYLYKKEEIKYHVLTIKDEQYFYNKKYDEDFKDVIGQ
                     DMAKYAALICAAGNHNFLMEGSPGCGKSMIAKRLQYILAPMNLEEILEKAKLQALDLK
                     DVDFSPIRAFRNPHHSSTKSSIFGGGSSNAKMGEVALSNHGVLFFDELPHFPSNILEA
                     LREPLEDNKILISRVNSKILYETKFIFVSAMNPCPCGNKLSSVKECRCSDFEVQRYKN
                     RLSEPFLDRIDLYVVMNDSFSDNKNIVSSKELHESVIKAFIRQKNRGQKELNGKLNDK
                     DIKKYCVLDSETSDILEKARVNYSLSFRSINKILKVARTIADINENEFITKGDLLKSL
                     NFRKR"
     gene            1271..2530
                     /locus_tag="PKO10_02050"
     CDS             1271..2530
                     /locus_tag="PKO10_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Na+/H+ antiporter"
                     /protein_id="HNW65971.1"
                     /translation="MSPSTLQIIAVTIFTLAIIHIFSVKYFEHLAHRSEKHSGLFHLL
                     GEIEVVFGIWAMILVLFMFIFLGKEETMNYVNNRNYVETMFVFVIMVIAATRPILNTV
                     LSLVKRVSNILPLKGATSFYFVVMCLVPLLGSLITEPAAMTLAALILSDKLFSQGISN
                     KLKYITLGALFVNISVGGTLTNFAAPPILMVAKTWEWDTIFMFTTFGWKAIIITFINT
                     ILIIVVFYKELSNINIRTTVSDREKIPFAIILVHFIFLFLVVYFGHYPAFFMSIFLLF
                     LGVTYAYQQYQDRLMLREGLLVAFFLGGLVILGGQQEWWLKDIISNLSNPEAFLGAIT
                     LGAFTDNAAITYLGSLVEGLSDEFKYYLVAGAVTGGGLTIIANAPNPAGAAILKSHFQ
                     DNSIDPRFLFLGALVPTIISSLCFIFL"
     gene            2543..>2801
                     /locus_tag="PKO10_02055"
     CDS             2543..>2801
                     /locus_tag="PKO10_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW65972.1"
                     /translation="MVCSFAKFARTNKSLIVFSNSFDHIEVFKKFYKEKVKPNEFGFF
                     KVNDDRICLLHEDLKCDLIQGKTTEALREKLLESFVINSRYI"
BASE COUNT         1024 a          294 c          489 g          994 t
ORIGIN      
        1 agcacttgat ggaacaataa aagatacaag tcatattttc gcaatagttt tatctttggc
       61 aaaaaaagga gagttaaaaa gagttgtaag ctctttggaa agtgctaaaa aattaggaaa
      121 tataccaaat attgatattt atgttgtaaa taccttaaat agcgcaatag agtttgtaaa
      181 aagtaaagat aaagataact atttgtataa aaaagaggag attaaatatc atgttttaac
      241 tataaaagat gaacaatatt tttataataa aaaatatgat gaagatttta aagatgttat
      301 aggtcaagat atggcgaaat atgcagcact tatttgtgca gctggaaatc ataatttttt
      361 gatggaagga agtccaggtt gtgggaaatc aatgatagca aaaaggttac aatatatttt
      421 agctcctatg aatcttgaag agatacttga aaaggcaaaa cttcaagctc ttgatttaaa
      481 agatgttgat tttagcccta taagagcttt tagaaatcca catcattcaa gtacaaaatc
      541 ttctatattt ggtggaggaa gctcaaatgc taaaatggga gaagttgctt taagcaacca
      601 cggagttcta ttttttgatg agttaccaca ttttccaagc aatattttag aagctttaag
      661 agaaccactt gaagataata agatattaat ttcaagagta aatagtaaga tactttatga
      721 gacaaaattt atatttgtaa gtgcgatgaa tccatgtcct tgtggaaata aactttcaag
      781 tgttaaagag tgtagatgta gtgattttga agtacaaaga tataagaacc gtctaagtga
      841 gccattttta gataggattg atttatatgt tgttatgaac gatagtttta gtgataataa
      901 aaatatcgta agttcaaaag agcttcacga aagtgtaata aaagctttta taagacaaaa
      961 aaacagagga caaaaagagt taaatggaaa attaaatgat aaagatataa aaaaatattg
     1021 tgtattggat agtgaaactt cagatatact tgaaaaagct agagtaaatt actctttatc
     1081 atttagaagt ataaataaaa tattaaaagt ggcaagaaca attgctgata taaacgaaaa
     1141 tgagtttata actaaaggtg atttattaaa aagcttaaat tttagaaaaa gataaaaata
     1201 tatattttgt ttaattacta ttaatatttt ttttgtaaaa tcggacaaaa tttgcgttaa
     1261 ggattaaaat atgtcaccat caacactgca aattatcgct gttactatct ttacattagc
     1321 aattattcat attttttcag ttaaatattt tgaacattta gcacacagaa gtgaaaaaca
     1381 ttcaggactt tttcatcttc ttggagagat tgaggttgta tttggtattt gggctatgat
     1441 tttagtttta tttatgttta tatttttagg aaaagaagag acaatgaact atgtaaataa
     1501 tagaaattat gtagaaacaa tgtttgtatt cgtaatcatg gttattgcag ctactagacc
     1561 tattttaaat acagtattat ctttggtaaa aagagtatca aatattttgc cacttaaagg
     1621 agctacttct ttctattttg ttgtaatgtg tttagttcca cttttaggct cactaatcac
     1681 tgaaccagca gctatgacac tagctgcact gattttaagt gataaactat tttcacaggg
     1741 tatttcaaat aagttaaaat atataacttt gggggcttta tttgtaaata tatctgttgg
     1801 aggaactttg acaaattttg cagctcctcc tattttaatg gttgcaaaaa cttgggagtg
     1861 ggacacaatt tttatgttta caacttttgg ttggaaggca ataattatta cttttattaa
     1921 tacaattttg attatagttg tattttataa agaactttca aatattaata taagaacaac
     1981 agttagtgat agagaaaaga taccttttgc tataattcta gttcatttta tatttctttt
     2041 tttagtggta tattttggac attatccagc attttttatg agtatatttt tgctattttt
     2101 gggagttaca tatgcttatc aacagtatca agatagatta atgctaagag aaggtctttt
     2161 ggttgcattc ttcttgggtg gtttggtaat attaggtggt cagcaagagt ggtggctaaa
     2221 agatattatc tcaaatttga gtaacccaga agcattttta ggagctataa cactaggagc
     2281 atttactgat aatgctgcta ttacatattt aggttcttta gttgaaggat taagtgatga
     2341 gtttaagtac tatttagtag caggagctgt tacaggtggt ggattgacta ttattgcaaa
     2401 tgcaccaaat ccagcaggtg cagcaatact taaaagtcat tttcaagata actctattga
     2461 tccaagattt ttatttttag gagcattagt tcctacaata atatcaagtt tgtgttttat
     2521 atttttataa ggataagaaa aaatggtttg tagttttgct aaatttgcaa ggacaaataa
     2581 gagtttaata gttttttcaa actcatttga tcatattgaa gtttttaaaa agttttataa
     2641 agagaaagtt aagccaaatg agtttggttt ttttaaagta aatgacgata ggatttgtct
     2701 attacatgaa gatttaaaat gtgatttgat acaaggaaaa acaacagagg ctttaagaga
     2761 aaagctttta gaatcttttg ttataaattc taggtatatt a
//
LOCUS       DAOQAN010000084         2797 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_50392_length_2797_cov_2.718454, whole genome shotgun sequence.
ACCESSION   DAOQAN010000084 DAOQAN010000000
VERSION     DAOQAN010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50392_length_2797_cov_2.718454"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="PKO10_02060"
     CDS             complement(<1..323)
                     /locus_tag="PKO10_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter"
                     /protein_id="HNW65973.1"
                     /translation="MKKSIFGIAAAAILATSSFGADYVMKISHVVSSSTPKGMAADFL
                     EKRIEELTAGKIDVQVFPNSQLYGDSDEMKALAMNNVQLIMPSLSKFPSIVPQIQLFD
                     LPFLF"
     gene            534..1316
                     /locus_tag="PKO10_02065"
     CDS             534..1316
                     /locus_tag="PKO10_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNW65974.1"
                     /translation="MKFFIKILKDFPSYLWGGWASVASIFLFFALWDFGHQIYGDLIL
                     PSPKDTFIAMSSILQDSSMQNEIMITLKRALSGFGLAILIGSILGLLAGLFVTASIMS
                     RPIVTILFGMPPIAWIVLAMIWFGVSDTTVIFTVFIASFPIVFVGALQGTRTIEGDLK
                     QMADSFNLPFSMKLFDIYFPHIFSYIFPAYISALGMSWKIVIMAELLATSDGLGSSLA
                     IARSQLETSTALAIVAIMIGALLLIEYIVLEPIKKELELWRK"
     gene            1304..2071
                     /locus_tag="PKO10_02070"
     CDS             1304..2071
                     /locus_tag="PKO10_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNW65975.1"
                     /translation="MEKISNKNFIEELVVENVNFSFGFKEILKDINFTLKKGEVVSIV
                     GPSGGGKTTLLHLCSKLLKLDTGKIKNSFLSSTFAFQEPRLLPWKNVIDNISLALKAK
                     GEKTNIAEQKAKDIAILFGLQESDFSKFPKDLSGGMKQRVSFARALVVNPSLLFLDEP
                     FSALDIGLKKELQNLLIKNIENNNLSILFITHDLMEAIKLSDKIIVLKAQPIGHIKKI
                     FTINEAREKRDDEFVYSKTAEILKDKDIISSFELELK"
     gene            2068..>2797
                     /locus_tag="PKO10_02075"
     CDS             2068..>2797
                     /locus_tag="PKO10_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HNW65976.1"
                     /translation="MSSSHYLNYPKGDFPIYLAYGFRPIFLLLAPYIVLSIILWAFVF
                     AGYINLPIENSLNWHIYEMIFGVGTAMIVAFFLTGLPELFPGVIPIVGKHLAFIVFWW
                     ILGRFSFWFIDYFGIFFTGFINISLTAYISYLAAIPAFRDRNKRHTSLAYSMVSIVII
                     QAIFFLSESKILNIDSYKILLLSMILFLVLILLALRRVSMESINELLNQENINEIFLA
                     KSFRYNLAIFCLLLYGFVELFFPNN"
BASE COUNT          946 a          362 c          439 g         1050 t
ORIGIN      
        1 ctaaataaaa atggtaaatc aaaaagttga atttgtggaa caatcgatgg gaattttgat
       61 aaacttggca taattaattg aacattgttc atagccaaag ctttcatctc atctgaatca
      121 ccataaagtt gtgaatttgg gaaaacttga acatcaattt ttccagcagt taactcctca
      181 attctttttt ctaagaaatc agcagccata ccttttggcg tactagaact tacaacgtga
      241 cttattttca taacatagtc agcaccaaag cttgaagttg caagtattgc agctgcagct
      301 attccaaata tacttttttt cataaatact cctaaaattg aaagtttgat ttcaagttta
      361 tccaaaaatt atgtaaatgt tgtgtcacta tagataaatt tttgataaat tactattttg
      421 tttaaaaaaa cttaagctat tttaaagaaa ctagctattc tcttgaccaa actcaatgat
      481 aattaatatc attttataat ataattccaa ttatattaaa ttatggaaat aaaatgaaat
      541 tctttattaa aatcttaaaa gattttccat cttatctttg gggaggatgg gcatctgttg
      601 cttcaatatt tctatttttt gctttgtggg attttggaca ccaaatttat ggagatttaa
      661 ttcttcctag tccaaaagat acatttatag caatgagctc tatattacag gatagctcaa
      721 tgcaaaatga gattatgata actctaaaaa gagcattaag tggttttggt ttagcaattt
      781 tgattggctc tattttagga cttcttgcag gactttttgt aactgcatct attatgagcc
      841 gtccaatagt cacaattctt tttggaatgc caccaattgc ttggatagtt ctagcaatga
      901 tttggtttgg tgtgagtgac acaacagtta tttttacagt atttattgca tcttttccta
      961 ttgtttttgt aggtgcttta caaggaacta gaactattga aggtgattta aagcagatgg
     1021 ctgatagctt taatttacca ttttctatga aactttttga tatatatttt cctcatattt
     1081 tttcatacat ctttccagca tatataagtg cattaggaat gtcttggaaa atagtaatta
     1141 tggcagaact attagcaaca agtgatggat taggttcaag tttagcaata gcaagaagcc
     1201 agcttgaaac atcaacagct ttggctattg ttgctattat gataggggct ctacttttaa
     1261 tagaatatat agttttagaa ccaattaaaa aggagcttga actatggaga aaataagtaa
     1321 taaaaacttt atagaagagc ttgttgtaga aaatgttaat ttctcttttg gttttaaaga
     1381 gattttaaaa gatataaact ttactttaaa aaaaggagaa gtggtatcta tagttggacc
     1441 tagtggtggt ggcaaaacta ccctacttca tctttgttca aaacttttaa aacttgatac
     1501 aggaaagata aaaaatagtt ttttatcctc tacttttgct tttcaagaac cacgactttt
     1561 gccttggaaa aatgtgattg acaatatttc tttagctctt aaagcaaaag gtgaaaaaac
     1621 aaacatagca gaacaaaaag caaaagatat tgcaatttta tttggcttac aagagagtga
     1681 tttttctaaa tttccaaaag atttaagtgg tggaatgaaa caaagagtat catttgctag
     1741 agctttggtt gtaaatccta gtttgctttt tttagatgaa ccattttcag ctttagatat
     1801 aggtcttaaa aaagagcttc aaaatttatt aataaaaaat atagaaaata ataatttgag
     1861 tatccttttt attacgcacg atttgatgga agctataaaa ctaagtgata aaataattgt
     1921 tctaaaagca cagccaattg gtcatatcaa aaaaatattt actataaatg aagcaagaga
     1981 aaaaagagat gatgaatttg tatatagtaa aactgcagag attttaaaag ataaagatat
     2041 tatctctagc tttgaattgg agttaaaatg agcagttctc actatttaaa ctatccaaaa
     2101 ggtgatttcc caatatattt ggcatatgga tttcgcccta tttttcttct tcttgctcca
     2161 tatattgtat taagcattat tttatgggct tttgttttcg caggttatat aaacttacca
     2221 attgaaaata gtttaaattg gcatatttat gagatgattt ttggagttgg aactgctatg
     2281 atagtggcat tttttctaac tggtttacct gaactttttc ctggagttat tccaatagtt
     2341 ggaaaacatt tagcttttat agttttttgg tggattttgg gaagatttag cttttggttt
     2401 atagactatt ttggaatatt ttttacagga tttataaata ttagtttaac ggcatatata
     2461 tcatatcttg cagcaattcc agcttttcgt gatagaaata aaaggcatac ttctcttgca
     2521 tactctatgg tttcaatagt tattattcaa gctatctttt ttttaagcga atcaaaaatt
     2581 ttaaatatag attcatataa gattcttctt ttatctatga ttttattttt ggttttaatt
     2641 ttattggctt taagaagagt tagcatggag tcaataaatg agttattaaa tcaagaaaat
     2701 ataaatgaaa tatttttagc aaaatctttt agatacaacc ttgctatatt ttgtctcctt
     2761 ttatatggtt ttgttgaact cttttttcca aataatt
//
LOCUS       DAOQAN010000085         2792 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_50531_length_2792_cov_2.604677, whole genome shotgun sequence.
ACCESSION   DAOQAN010000085 DAOQAN010000000
VERSION     DAOQAN010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50531_length_2792_cov_2.604677"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(195..1001)
                     /locus_tag="PKO10_02080"
     CDS             complement(195..1001)
                     /locus_tag="PKO10_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNW65977.1"
                     /translation="MPLVKTNLDKLNFSKLYKIQMKNSTFKSKSSSDWDKKALHFSQN
                     VLNSPYTKEFTKRVDISDCETLLDVGSGPATISLALAKKLKTVYALDYSKEMLNYAEQ
                     NAKNKEIDNLVSIHKSWYDSWEDVPNADIVVASRSMEVKDIKKALKKLNEKANKRVYI
                     TTKVGGSFIDKEILKQLKRDIIPRPDYIYLVNTLHSMGIYAKVDFIETKSSKFDTLDE
                     DEFIQSLKWSLGDVSKKEEKILREYFNTTYKNKKEKESLTWAFISWQKSL"
     gene            complement(1012..1866)
                     /locus_tag="PKO10_02085"
     CDS             complement(1012..1866)
                     /locus_tag="PKO10_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNW65978.1"
                     /translation="MISLNIKKELHGSNGVMNLDINLSLQNGEFVALSGVSGSGKTTL
                     LRVLAGLEEAFGEIIVDGEIWLNEKIKKPIQKRDIGFVFQDYALFPNLSVIDNLLYVK
                     KDKDLAKQLLSLTDLYELKNRYPNSLSGGQKQRVSLCRALMKRPKILLMDEPLSALDP
                     HMRLKLQDEILTLHKEFKTTTIMVSHDPSEMYKLASRVLVLKDGKIIDDGLPKDILLK
                     TQGSQKFSFEGELLDIIKVDVINIAIVAIGQQIVEVVISNIEAQNLIIGEKVNVSTKA
                     FSPTIKKI"
     gene            complement(1863..2558)
                     /gene="modB"
                     /locus_tag="PKO10_02090"
     CDS             complement(1863..2558)
                     /gene="modB"
                     /locus_tag="PKO10_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014472743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HNW65979.1"
                     /translation="MIDYLLSVDYAPFLLSFKLAFITTLILFVLCLPLSWYLSQTKSK
                     AKPFLEALCTMPLVVPPTVLGFYLLWGLSHNSPIGQFFNDYFGIKLVFNFYGIIIASC
                     IYSLPFMVNPLQSGFESLNKNMLEASYISGKGKFKTLLFIALPNIKPSLLTALIITFA
                     HTVGEFGVVLMIGGSIPNETRVAAIAIYEFVEILDYKNAHIYSLIMIILSFITLLAVY
                     IFNGKQKKIGIVK"
     gene            complement(2551..>2792)
                     /locus_tag="PKO10_02095"
     CDS             complement(2551..>2792)
                     /locus_tag="PKO10_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014472744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HNW65980.1"
                     /translation="VIISKNYIEDISLSNQTLAKIVAIENGELLSSISLKIGDTTFES
                     IITKESSKRLDLQEGNIVNILIKASDLSILRVLHD"
BASE COUNT          955 a          462 c          307 g         1068 t
ORIGIN      
        1 cgtgtaaact acctttaaat ctttttctta ctttgtgtat ttttccataa tttcactatt
       61 gtgattatct atctatgaga aaaatataca aaattgagga taaatctcac tagagtctct
      121 aataatactt tttgtctatt ttacttgaag attttgtgtt gagatataat attttaaaga
      181 cttaatctat tgttttataa actcttctgc caagagataa atgcccaagt taaactctct
      241 ttctcttttt tatttttata agtagtatta aaatactctc ttaatatttt ctcctctttt
      301 tttgatacat ctcctaaact ccattttaag ctttgaataa attcatcttc atctaaggta
      361 tcaaattttg aagattttgt ctcaataaaa tcaacctttg cataaattcc catactatga
      421 agagtattta ctaaatagat ataatcaggt cgtggaataa tatctctttt taattgcttt
      481 aaaatctctt tgtctataaa acttccaccc acttttgttg tgatatagac tcttttattt
      541 gctttttcat ttaatttttt taaagctttt tttatatctt ttacttccat agacctactt
      601 gcaactacaa tatcagcatt tggaacatct tcccaagagt cataccaaga tttatgaatg
      661 cttactaaat tatcaatttc tttgtttttt gcattttgtt cggcataatt taacatctct
      721 tttgaataat caagagcata aacagttttt aatttttttg caagagccaa actaatagta
      781 gcaggtccac ttccaacatc aagcaaagtt tcacaatcgc ttatatcaac tctttttgta
      841 aactcttttg tatatggact atttaaaaca ttttgactaa aatgcaaagc ctttttatcc
      901 caatcactac tacttttact tttaaaagta gagttcttca tctgtatttt atatagtttt
      961 gaaaagttca atttatctaa atttgttttt acaagtggca tttttaatcc tttaaatctt
     1021 tttgatggtt ggagaaaatg cttttgtact tacatttact ttttctccaa ttattaaatt
     1081 ttgagcttct atatttgaaa ttacaacttc tacaatttgc tgaccaatag caacaatagc
     1141 gatatttata acatcaactt ttataatatc aagaagttct ccctcaaaac taaatttttg
     1201 gcttccttga gttttaagta aaatatcttt tggtaagcca tcatctataa tttttccatc
     1261 ttttaaaact aaaactctag atgctaattt atacatctca cttggatcat gacttaccat
     1321 aatagttgta gttttaaact ctttatgaag tgttaaaatc tcatcttgaa gttttaatct
     1381 catgtgggga tcaagagctg aaagtggttc atccataaga agtatttttg gtcttttcat
     1441 taaagcacga caaagactta cacgctgttt ttgaccacca cttaaagagt ttggatatct
     1501 atttttaagc tcatacaaat ctgttaagct tagaagttgt ttagctaaat ctttatcttt
     1561 ttttacataa agcaaattat caattacact taaatttgga aatagtgcat aatcttgaaa
     1621 aacaaatcct atatctcttt tttgaatagg ctttttaatc ttttcattta accaaatttc
     1681 accatctaca ataatctctc caaaagcttc ttcaagacca gcaagaactc ttaaaagagt
     1741 agttttccca cttccactaa ctccacttaa tgctacaaat tcgccatttt gtaaagatag
     1801 atttatatct aaattcataa ctccatttga accgtgaagc tcttttttta tatttaaact
     1861 tatcatttca caattccaat cttcttttgt tttccattaa aaatgtaaac tgcaagtaaa
     1921 gttatgaaac taagaataat cataattaaa ctataaatat gtgcattctt ataatctaaa
     1981 atctcaacaa actcataaat tgcaattgct gctactcttg tctcatttgg aatacttcca
     2041 ccaatcatca aaacaacccc aaactctcca acagtatgag caaaagttat gattaaagct
     2101 gttaaaagtg aaggctttat atttggtaaa gcaataaata aaagggtttt aaatttacct
     2161 ttcccactaa tataactagc ctcaagcata tttttattta agctttcaaa gccactttga
     2221 agaggattta ccataaaagg aagactataa atacaactag caataataat tccataaaaa
     2281 ttaaatacaa gttttattcc aaagtaatca ttgaaaaatt gtccaattgg agaattatgt
     2341 gataatcccc aaagaagata aaatcctaaa acagtaggag gaacaactaa aggcatagta
     2401 caaagtgctt ctaaaaatgg tttagctttt gactttgttt gtgataaata ccagctaagt
     2461 ggcaaacata gcacaaacaa aatcaaagta gtaataaatg ccaatttaaa tgagagcaaa
     2521 aatggtgcat aatctacact taatagataa tcaatcatgt aataccctta aaattgataa
     2581 atcactagct tttattaaaa tatttacaat attcccctct tgtaaatcaa gtctttttga
     2641 agactctttt gtgattatac tttcaaaagt agtatctcct atttttaaag agatactact
     2701 taataactct ccattttcta tagcaactat ttttgcaaga gtttggtttg ataaactaat
     2761 atcttctata tagttttttg aaattattac at
//
LOCUS       DAOQAN010000086         2785 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_50725_length_2785_cov_2.711355, whole genome shotgun sequence.
ACCESSION   DAOQAN010000086 DAOQAN010000000
VERSION     DAOQAN010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50725_length_2785_cov_2.711355"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..843)
                     /locus_tag="PKO10_02100"
     CDS             complement(<1..843)
                     /locus_tag="PKO10_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW65981.1"
                     /translation="MKKLLIGFVVLIVAGAIFFTINRSVDKAVNLKIEELNQNGFSIT
                     QNNSNLPMKIRKDGEIQVIDAIKALDFIVKNIEESQAKEVFVEFLNIFDSQSKQLALE
                     GTKFDYDFSLNIFTKEMNADLYLKELSVVLQNELENSEDEASKELLSILKQKAIHLKV
                     DDKMNFTLDDIAFSNSGSLISLRGINGDKNSLNIALFKIIGANNESFVLEDMKSYYKE
                     IEKNIDTKFSVSNLSLESEFVKMGIKNILFDGSSKNINDKVSTKDKISFDEFSFISND
                     VQSLI"
     gene            complement(889..1089)
                     /locus_tag="PKO10_02105"
     CDS             complement(889..1089)
                     /locus_tag="PKO10_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011139225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent tyrosine transporter"
                     /protein_id="HNW65982.1"
                     /translation="MFVQLNERVLLNLSKITRTKIDHVEDGIRVRFYEGQYQVAKSKR
                     FETVEDANKWLFELLKPFNSQN"
     gene            complement(1135..2052)
                     /locus_tag="PKO10_02110"
     CDS             complement(1135..2052)
                     /locus_tag="PKO10_02110"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNW65983.1"
                     /translation="MLEENKIIGFFGPNGSGKSTLFSILSFISKPQNGEIIIDENIKK
                     SVVMLPQNPYLLKRTVFENIAYGLKIRGESVDLKSKVEEALSLVGLDKNFSNRKWNQL
                     SGGEAQRVALASRLILKPKVLILDEPTVGVDTNSAQLIKDAILLAKQKYNTTIFISSH
                     DYTWLKHISDKKIALFQGSLVEHGNINLLFPPWSKNQNGDLVKNFIDGQKIVIPNSKS
                     KKRDSVVIINQSSIKISKFDENNKKERLIATVTSIQKDENRDNLQIEFSICGINFSCL
                     ISKNEIQKDILFPGDKVEVFFDLDSVCWI"
     gene            complement(2123..>2785)
                     /locus_tag="PKO10_02115"
     CDS             complement(2123..>2785)
                     /locus_tag="PKO10_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002781017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNW65984.1"
                     /translation="IELLISGNDSVYSAIIVTITVSSWSILISLILGLPLGFLLGYYQ
                     FKGKTVIKTIVDTFLAFPTVVIGLLVYTTLSQAGAFGEYNLLFTQKAIIIGQIFLGLP
                     IIIALTAAQVESTDKRLYLSLKGLGAKPYQILLTTLVEARFGLMTAAMTAYGRIITEI
                     GISMMVGGNIKYHTRTITTAISLETGKGEFETGIALGLVLFIVALMVNIALSMLKRRW
                     TQ"
BASE COUNT          948 a          449 c          282 g         1106 t
ORIGIN      
        1 tatcaagctt tgaacatcat tactaataaa agagaactca tcaaaagaga ttttatcttt
       61 tgtactaact ttgtcattta tatttttaga agagccatca aataagatat tttttatacc
      121 catttttaca aattcacttt ctagtgatag attagaaact gaaaatttag tatctatatt
      181 tttttcaatc tctttgtaat agcttttcat atcttctaag acaaagcttt cattatttgc
      241 accaataatt ttaaataaag caatatttaa actattttta tctccattta ttcctcttaa
      301 tgatattaaa gagccactat tactaaatgc aatatcatct aaagtaaagt tcattttgtc
      361 atctactttt aagtgaatag ccttttgttt taagatactt aaaagctctt tactagcttc
      421 atcctcacta ttttctaact cattttgtaa tacaactgat aactctttta agtataaatc
      481 tgcattcatc tcttttgtga aaatatttaa agagaaatca taatcaaact ttgtaccctc
      541 aagtgctaac tgttttgatt gactatcaaa aatatttaaa aactcaacaa aaacctcttt
      601 tgcttggctc tcttctatat tcttaactat aaaatctaat gcttttatag catctataac
      661 ttggatttca ccatcttttc ttatcttcat tggaagatta gaattgtttt gagttataga
      721 aaaaccattt tgatttaact cttcaatttt tagattaact gccttatcaa ctgacctatt
      781 tatagtaaaa aaaatagctc cagctactat taaaactaca aatcctatca acaatttttt
      841 catttatgtt cctttttctt taaaaacaaa agcttatcaa ataatttttt aattttgtga
      901 attaaaaggt tttaataatt caaaaagcca tttattagca tcttcaactg tttcaaatct
      961 ttttgacttt gcaacttgat attgaccttc gtaaaatcta actcttattc catcttcaac
     1021 atgatctatt ttcgttctag ttattttaga tagatttaga agaactcttt cgtttaattg
     1081 tacaaacata tttgctccta attttaaata gttgtaagtt tactatttta tatcttatat
     1141 ccaacaaaca ctatctaaat caaaaaatac ttctacttta tcccctggga ataatatatc
     1201 tttttgaatc tcattcttag agattaaaca agaaaaatta ataccacaaa tggaaaattc
     1261 tatttgtaaa ttatctctgt tttcatcttt ttgtatagat gttacagttg ctatcaatct
     1321 ttctttttta ttattttcat caaatttaga tatttttata ctactttgat tgattattac
     1381 aactgaatct ctttttttac tcttagagtt tggaattact attttttgac catcaataaa
     1441 atttttaacc aaatcaccgt tttgattttt actccatggt ggaaatagta gatttatatt
     1501 tccatgttca actaaacttc cttgaaaaag tgctatcttt ttatcactta tatgttttaa
     1561 ccatgtataa tcgtgacttg atataaatat tgtagtgtta tatttttgct ttgccaataa
     1621 aatagcatct ttgattaatt gtgctgaatt tgtatcaact ccaactgttg gttcatctag
     1681 tattaatact tttggtttta aaattaatct tgaagccaaa gcaactcttt gagcttcacc
     1741 acctgagagt tgattccatt ttctatttga aaaattttta tcaagaccaa ctaaagagag
     1801 agcctcttca actttacttt ttaaatctac actctctcct cttattttta aaccataagc
     1861 aatattttca aatactgttc gttttaataa atatggattt tgaggaagca taacaacact
     1921 ttttttaata ttttcatcta ttatgatttc accattttgt ggtttagaaa taaaagataa
     1981 aatagaaaaa agtgtagatt ttccactacc atttggtcca aaaaaaccaa taattttatt
     2041 ctcttctaat ataagattat caatacttaa aactcttttt catcgaaaaa atactcaata
     2101 ttttttaatt catataaaat actcattgag tccatctcct ttttagcatt gataatgcta
     2161 tattaaccat aagtgcaact ataaaaagta ccaaacctaa agcgatacca gtttcaaatt
     2221 ctccttttcc agtttcaagt gaaattgcag ttgtaattgt tcttgtatga tattttatat
     2281 ttccaccaac catcatagaa attccaattt cagtgataat tcttccataa gcagtcattg
     2341 ctgctgtcat tagaccaaat ctagcttcaa ctaaagttgt taataaaatt tgatatggtt
     2401 ttgcacccaa accttttaaa gatagatata atcttttatc tgttgattca acttgtgcag
     2461 ctgttaatgc aataattata ggtaaaccta aaaaaatctg ccctataatt attgcttttt
     2521 gagtaaatag tagattatac tctccaaaag ctcctgcttg tgatagagtt gtatatacaa
     2581 gaagtcctat tacaactgta ggaaatgcca aaaatgtatc tacaatagtt tttattactg
     2641 tttttccttt aaattgataa taacctagta aaaatcccaa aggaagtcct agtattagac
     2701 taatcaatat tgaccaagaa gaaactgtaa tagtaacaat tattgcagaa tatacactat
     2761 catttccact aattaaaagc tcaat
//
LOCUS       DAOQAN010000087         2781 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_50838_length_2781_cov_2.377843, whole genome shotgun sequence.
ACCESSION   DAOQAN010000087 DAOQAN010000000
VERSION     DAOQAN010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50838_length_2781_cov_2.377843"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="PKO10_02120"
     CDS             <1..306
                     /locus_tag="PKO10_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014469401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C biogenesis protein CcsA"
                     /protein_id="HNW65985.1"
                     /translation="EVASKYKFTNVGDFKGDEKGMVKTPTLRNITETAPYFHNGQIWS
                     LANAVKEMGSVQLGINISDDDAAKIVTFLKALKGTKPTITYPQLPESTDSTPKPSFD"
     gene            398..1540
                     /locus_tag="PKO10_02125"
     CDS             398..1540
                     /locus_tag="PKO10_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013136702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNW65986.1"
                     /translation="MKSNLLIIIYGLIVTMSVMYATQPLQPLLASKFNISIIEASQFT
                     AVILFFLAVAPIIYGYILEKVNAKKMLINASIVLLITNILLGLSQNYELFLFFRVCEA
                     LVIPAILTSLMSILANIDSENIKFNMSIYVAGTVFGGLVGRIFSGFIATTFSYEYVFY
                     SLSVAILISILLIRKLDFNGDANIIKPRISDVINILKDKRFLIIYFIMFFIFFVFSGV
                     LNVLPFRAKEISNNVSEFQIALLYLGYGMGILVSLTSKKIVSFFKGEINTIFIAIIFY
                     IFSIIFLLNENILYLFIFLFLVCIGMFTTHTVSTQLANSMKSSQKSLTSGMYLTFYYL
                     GGASGSIIPSYIYKAFGWNIMLTIFIFLIIIVALVVFFNRKLFKTI"
     gene            complement(1523..2086)
                     /gene="maf"
                     /locus_tag="PKO10_02130"
     CDS             complement(1523..2086)
                     /gene="maf"
                     /locus_tag="PKO10_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014769896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HNW65987.1"
                     /translation="MIRLGSNSPTRAKILEAFNIEFIQNGGNFDEDSIKTTNPKEFCY
                     LATKGKFDELYSKFGVEDFPLLVADSVVTSQAKLLRKPKDYNDAKYMLELQSGSKTSV
                     ISCFIFKSKNIELIDISITTYDFMEFNKKDMEEYLKSGECFGKAGAIMVEGFCKPYIK
                     SVKGLESTAMGLSIEKLIPFLNSNSFK"
     gene            2155..>2781
                     /locus_tag="PKO10_02135"
     CDS             2155..>2781
                     /locus_tag="PKO10_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014469404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance protein TerC"
                     /protein_id="HNW65988.1"
                     /translation="MLYLSRFGGVFIFLSFIFSIFSYFFDSKLKIISVVFIWFASLIL
                     FFTIKSKKLILTLLFFTTIAFLYSYFNNFYIDIEKSFSVNLYLLTLLISVGFLKLITA
                     PKKDKEELPRGKISFIKTYLGIHLFGSIINLSALLLVADKMYKKSKLSPLQIIVLTRS
                     FASDAYWSPFFVAFAAALTYAPNLNAFSIISFGTVIAFIAFFITYLEVI"
BASE COUNT          933 a          370 c          341 g         1137 t
ORIGIN      
        1 gaagtggctt ctaaatacaa atttacaaat gtaggtgatt tcaaaggtga tgaaaaagga
       61 atggttaaaa caccaactct tagaaatatt actgaaactg caccttattt ccacaatgga
      121 caaatttggt cacttgctaa tgcagtaaaa gagatgggtt ctgtacaatt gggaattaat
      181 atatcagatg atgatgcagc taaaattgta acatttttaa aagcgctaaa aggtactaaa
      241 ccaactatta cttatccaca acttccagaa tcaacagatt ctactccaaa accatcattt
      301 gattaataaa attagggctt aagccctaat tttaaccttt tacttaacta taaacaaata
      361 cttttaagag taaaatccac ttttaaaagg atattatttg aaaagtaatt tgttgattat
      421 tatttatggt cttattgtta caatgtctgt tatgtatgca acacaacctc ttcagccgct
      481 tcttgcatca aaatttaata tatctattat agaagcttca caatttacag cagttattct
      541 atttttccta gcagttgctc caataattta tggatatatt ttagaaaaag taaatgcaaa
      601 gaaaatgtta ataaatgctt caatagtttt gcttattaca aatattttat taggattgtc
      661 tcaaaattat gaactatttt tattttttag agtttgtgaa gccttagtaa ttccagctat
      721 tttgacatct ttgatgagta ttttggcaaa tatagatagt gaaaatataa aatttaatat
      781 gtctatttat gttgcaggaa cagtttttgg aggactagta ggaagaattt tttctggatt
      841 tattgctact actttttcct acgaatatgt attttattct ctatctgttg caatacttat
      901 ctctatttta ttaataagaa aattagattt taacggggat gcaaatatta taaaaccaag
      961 aatttcagat gttataaata tattaaaaga taaaagattt ttaataatct acttcataat
     1021 gttttttata ttttttgtat tttctggagt tttaaatgtt ttaccatttc gtgcaaaaga
     1081 gatatcaaat aatgtttcag agtttcaaat tgccctttta tatttgggat atggtatggg
     1141 aattttggta tctcttacat caaaaaagat tgtatcattt tttaaaggtg aaataaatac
     1201 aatatttatt gctattattt tttatatctt ttcaattatt tttttattaa atgagaatat
     1261 tttatatcta ttcatatttt tatttttagt ttgtatagga atgtttacaa ctcatacagt
     1321 tagtactcaa cttgcaaact caatgaaaag ttcacaaaaa tcacttacat ctggaatgta
     1381 tcttactttt tactatttag gaggagcaag tggttcaatt ataccttcat atatttacaa
     1441 ggcttttggt tggaatatta tgctaactat ttttatattt ttaattatta ttgtagcatt
     1501 agttgttttt tttaatagaa aattatttaa aactatttga atttaaaaat ggaattaatt
     1561 tttctatact aagacccata gctgtacttt ccaaaccttt tacgcttttt atgtatggtt
     1621 tacaaaaacc ttcaaccata attgcacctg cttttccaaa acactctccg ctctttaaat
     1681 actcttccat atctttttta ttaaattcca taaaatcata tgtcgtaata gatatatcaa
     1741 ttaactctat atttttactt ttgaaaataa agcaagaaat tacacttgtt ttacttccac
     1801 tttgaagctc taacatatat tttgcatcat tataatcttt tggtttacga agaagttttg
     1861 cttgggatgt tacaacacta tcagccacaa gcaaagggaa atcttctact ccaaattttg
     1921 aataaagctc atcaaatttt ccctttgtag ccaaataaca aaactctttt ggatttgtag
     1981 tttttatact atcttcatca aaattgccac cattttgtat aaattctata ttaaaagctt
     2041 ctaaaatttt agctcttgta ggagagtttg aacctaatcg tatcaaataa agtccttttt
     2101 tatttggatt atactaagtt tttatataat acgattttaa taagaaggtt ttaaatgctt
     2161 tatttgtcaa gatttggcgg agtttttata tttttaagtt ttattttctc aattttttct
     2221 tatttttttg atagtaaatt aaaaattatt tcagttgttt ttatttggtt tgcttcattg
     2281 atactatttt ttacaataaa atcaaaaaaa cttatattaa ctctactatt ttttacaact
     2341 attgcatttt tatatagcta ctttaataat ttttatatag atattgaaaa atcatttagt
     2401 gtaaatttgt atctcttaac ccttttaatt agtgttggat ttttgaaatt aataacagct
     2461 cctaaaaaag ataaagagga gttgcctcgt ggaaaaatct cttttattaa aacttatttg
     2521 ggaattcatc tatttggttc tattataaat ctatctgcac tacttttagt tgctgataaa
     2581 atgtataaaa aatcaaaact atcaccttta caaattattg ttttaacaag atcctttgca
     2641 agtgatgctt attggtcacc tttttttgta gcttttgcag cagctttaac atatgctcca
     2701 aatctaaatg ctttttcaat aatctcattt ggtacagtta tagcttttat agcatttttt
     2761 attacatatc ttgaagtaat a
//
LOCUS       DAOQAN010000088         2757 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_51502_length_2757_cov_2.498520, whole genome shotgun sequence.
ACCESSION   DAOQAN010000088 DAOQAN010000000
VERSION     DAOQAN010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51502_length_2757_cov_2.498520"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..564)
                     /locus_tag="PKO10_02140"
                     /pseudo
     CDS             complement(68..564)
                     /locus_tag="PKO10_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006655022.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
     assembly_gap    565..574
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<575..1055)
                     /locus_tag="PKO10_02145"
     CDS             complement(<575..1055)
                     /locus_tag="PKO10_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008337936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNW65989.1"
                     /translation="MFNGKNILITGGTGSFGKKYTEILLSKYRPNKIIIFSRDELKQY
                     EMAQEYSAKCMRYFIGDVRDNQRLKQATKDVDFIIHAAALKHVPIAEYNPMECIKTNI
                     DGAQNVIEAALENGVKRVIALSTDKAANPVNLYGATKLASDKLFVAANNLVGNQDIKF
                     "
     gene            1163..1351
                     /locus_tag="PKO10_02150"
     CDS             1163..1351
                     /locus_tag="PKO10_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW65990.1"
                     /translation="MSEVIPDDILKIQKKLASFEKDSRNYKKYTKILAKHIKTHTMRK
                     RVNSHIKVIETVKTLNQE"
     gene            1445..2494
                     /gene="recA"
                     /locus_tag="PKO10_02155"
     CDS             1445..2494
                     /gene="recA"
                     /locus_tag="PKO10_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013325912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HNW65991.1"
                     /translation="MDENQKKSLELAIKQIDKAFGKGTLIRLGDKEVIPTEAISTGSL
                     GLDLALGVGGLPKGRVIEIYGPESSGKTTLTLHAIAEAQKAGGVCAFIDAEHALDVKY
                     AKDIGVDIDNLLVSQPDFGEQALEILETVIRSGAVDLVVVDSVAALTPKVEIDGDMDD
                     QQVGVQARLMSKALRKITGLLSKMNCTVIFINQIRMKIGMTGYGSPETTTGGNALKFY
                     SSVRLDIRRIATLKQGENSIGNRVKVKVVKNKVAAPFKQAEFDIMFGEGISKTGELVD
                     YGVKLDIIDKAGAWFSYGDTKIGQGRENSKVFLRDNPAIAKEIEDKILNSMGINDAII
                     VGSIDSEDEASSLDD"
     gene            complement(2536..>2757)
                     /locus_tag="PKO10_02160"
     CDS             complement(2536..>2757)
                     /locus_tag="PKO10_02160"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511125.1"
                     /note="catalyzes the formation of biotin from dethiobiotin
                     and sulfur; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase"
                     /protein_id="HNW65992.1"
                     /translation="KLVELARSYLPNQMLMIAGGRELMFGENQYDVFKHGANALVVGD
                     YLTTGGASAEDDIKAVTALGYEIAFACHQ"
BASE COUNT         1013 a          422 c          403 g          909 t
ORIGIN      
        1 aaagaaaatg aagcaccaac tggctttgtt aaactaaaag attttttgaa aaatctgccc
       61 cacttgtaaa tttaatagtt ggttttatta catagtgatc attaaactcc aaagttagat
      121 gactatcatc tgctggacac attatttcat gaagtttttc accaggtcta attccaataa
      181 ttttatgact tagatttgga gctaatgcct ttgccatatc aactattttc attgatggaa
      241 tttttggtac aaaaatctct ccaccttgca ttctttcaaa gttttttaaa acaaaattca
      301 ctccatcttc aagtgttata aaaaatcttg tcattctctc atctgttatt ggaagctctt
      361 ttacaccttc acagattaat tttttgaaaa aaggaacaac cgaaccccta cttcctacaa
      421 catttccata tcttacaact gaaaatttta tatcactttt tccaactaaa ttatttgctg
      481 caacaaaaag tttatcacta gccaattttg tagcaccata aagatttaca gcaataccat
      541 tggcagattt gctatacgcc caacnnnnnn nnnnaaaatt taatatcttg atttcccaca
      601 agattattag cagcaacaaa aagtttatca cttgctaact ttgttgcacc atatagattt
      661 acaggatttg cagctttatc agttgaaagg gctattactc tttttacacc gttttctaag
      721 gctgcttcaa ttacattttg agcaccatca atatttgttt taatacactc cataggatta
      781 tattcagcta ttggaacatg ttttaaagca gctgcatgaa ttataaaatc aacatcttta
      841 gtagcttgtt ttaatctttg attatctctt acatccccta taaaatatct catacattta
      901 gcactatact cttgagccat ctcatactgt tttaactcat ctctactaaa aataattatc
      961 ttatttggtc tatattttga taataaaatt tctgtatatt ttttaccaaa gcttccagtt
     1021 ccaccagtta taagtatatt tttaccatta aacataataa agccttattt taatatttga
     1081 agtagattct atcaaatttt taatttattt aatattttat taaattatgc aataatagat
     1141 ttatattttt agaggaaaaa aaatgagtga agttatccct gatgacattt taaaaataca
     1201 aaaaaaactt gcatcttttg aaaaagactc aaggaattac aaaaaatata caaaaatatt
     1261 agcaaaacat ataaaaacac acactatgag aaaaagagta aactcccata taaaagtgat
     1321 tgaaacagta aaaactttaa atcaagagta aaatattaca aattgacata tttctaataa
     1381 cttttaaaaa attgtgctaa gatttcaaaa taaataaaca aagaaaacta aaaaaggaaa
     1441 taatatggat gaaaatcaaa aaaaatcact tgaacttgct attaaacaaa tagacaaagc
     1501 ttttggaaaa ggtacactta ttagattagg agataaagag gtaattccaa ctgaagctat
     1561 tagtacaggc tcacttggtc ttgatttagc tcttggagtt ggtggtttac caaaaggaag
     1621 agttattgaa atttatggac ctgaaagttc tggaaaaaca actttaacac ttcacgcaat
     1681 tgctgaagct caaaaagctg gcggagtttg tgcatttata gatgcagaac acgcacttga
     1741 tgtaaaatat gcaaaagata ttggtgtaga tattgacaat ttactagttt ctcaaccaga
     1801 ttttggtgag caagctttag agatacttga aactgttatt agaagtggtg ctgttgattt
     1861 agttgttgtg gattctgttg cagccttaac acctaaagta gaaattgatg gtgatatgga
     1921 tgatcaacaa gttggagttc aagctagact tatgagtaaa gctttaagaa aaataactgg
     1981 acttttaagc aaaatgaact gtacagttat atttatcaac caaataagaa tgaaaattgg
     2041 aatgacagga tatggaagcc cagagacaac aacaggtgga aatgcactta aattttactc
     2101 atcagtaaga cttgatatta gaagaattgc aactttaaaa cagggtgaga actcaattgg
     2161 aaatagagta aaagtaaaag ttgttaaaaa taaagttgct gctccattta aacaagctga
     2221 gtttgatatt atgtttggtg aaggaatttc aaaaactggt gagcttgttg attatggtgt
     2281 taaacttgat attattgata aagctggagc ttggtttagt tatggtgata caaaaattgg
     2341 acaaggaaga gagaactcta aagtcttctt aagagataat ccagctattg caaaagagat
     2401 tgaagataaa attttaaact ctatgggaat aaatgatgca ataattgtag gaagcataga
     2461 tagtgaagat gaagcatctt ctttagatga ctaattttaa aagataataa aataataaag
     2521 gctttaagcc tttatttatt gatgacatgc aaaagctatt tcatatccta aagccgtaac
     2581 agctttgata tcatcttcag cacttgcccc tccagttgtc aaataatctc caacaactaa
     2641 agcatttgca ccatgtttaa atacatcata ctgattttct ccaaacatca actctctacc
     2701 acctgcaatc attaacattt gatttggtaa atagcttctt gctaattcaa ccaactt
//
LOCUS       DAOQAN010000089         2756 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_51537_length_2756_cov_1.948538, whole genome shotgun sequence.
ACCESSION   DAOQAN010000089 DAOQAN010000000
VERSION     DAOQAN010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2756
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51537_length_2756_cov_1.948538"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..858
                     /locus_tag="PKO10_02165"
     CDS             <1..858
                     /locus_tag="PKO10_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HNW65993.1"
                     /translation="LKEIFDNARKICGNLPLAANILYAINDYGRVVRDACEAGANIII
                     TGAGIPTNMPEFTKDFPDVALVPIVSSARALKLICKKWQRYNKIPDAVIVEGPLSGGH
                     QGFKYEDCYKEEFQLENIVTPVIEEAKNWGNIPVIAAGGIWDKKDIDKFISLGCAGVQ
                     MATRFIGTFECDADPKFKDVLLNAKEEDIVLMSSPVGLPARGVKTNLQFSIENHTAPK
                     VQCISNCVAPCNRGHEAKLVGYCIADRLGAAYKGDVETGLFFSGSNGYKIDKIISVKE
                     LMEKLTKGE"
     gene            921..2654
                     /locus_tag="PKO10_02170"
     CDS             921..2654
                     /locus_tag="PKO10_02170"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNW65994.1"
                     /translation="MEQQYKEARIEFLKSSLRKDDNQKIDDLKKIIELGKKLNKDVRP
                     DEKKLAVLNKSIKVINNKNIESAQTSSNNKQNKEKQSNLLYSIKNVTTSNNSIVVDFN
                     VDIKEDDIKFFELKPSPLYRDVFDIKGYFKDALATKLAIQGVEQITIGQFQPDVLRIV
                     ISGNNNPETSYKITKRQLIIDVNSKQSTKKDEQIKEISKQKIEPNSNIQEMVDLKNSI
                     RSISSSGDKITIQFNKKISQKDLKYSTLKQNGNFEYIFDINGKYSYIEPTKLNLNSID
                     KVITTENKNSVRIRLINKNNPKIKYILKSNELTIEVLETQSEKVEVAKNSSDSNSKTN
                     LPTKVSKQVQNTTPKNAINKTIVIDAGHGGDDVGAVGPNKEYEKVINLEVAKYLDNIL
                     KQRGYKVYLTRSTDKFIKVMDRTILANEKNADLFISIHTNSMPKEKASSTSGIETFFL
                     SPARSERAKKVAALENKDDIREMSESSKNVFLESLNRPRITASHKFAIDVQSGLLQAA
                     RTKYKDVRDTGVKEGPFWVLVGEQMPSILIELGFISHPVESKRLYDKDYQQVLANGIA
                     NGIDSYFLKNP"
BASE COUNT         1118 a          326 c          448 g          864 t
ORIGIN      
        1 ttaaaagaga ttttcgataa tgctagaaaa atttgtggaa atttgccact tgctgcaaat
       61 attttatatg ctataaatga ttatggaaga gtagtacgtg atgcttgtga agcaggtgct
      121 aatataataa ttactggtgc tggaattcct acaaatatgc ctgaatttac aaaagatttc
      181 ccagatgttg cacttgttcc aattgtatca agtgcaagag ctttaaaact tatttgtaaa
      241 aaatggcaaa gatacaataa aataccagat gctgtaatag ttgaaggacc tttaagtggt
      301 ggtcatcaag gtttcaaata tgaagattgt tataaagaag agtttcaact tgagaatatt
      361 gtaactccag ttattgaaga agcaaaaaac tggggtaata ttcctgttat tgcagctggt
      421 ggaatttggg ataaaaaaga tattgataag tttatatctc ttggttgtgc tggagttcaa
      481 atggctacta gatttatagg aacttttgag tgtgatgctg atcctaaatt caaagatgtt
      541 ttactaaatg caaaagaaga agatattgtt ttaatgagtt ctccagtagg tcttcctgca
      601 cgtggagtta agacaaatct tcaattctca atagaaaacc atactgcacc aaaagttcaa
      661 tgcatctcaa attgtgttgc accttgtaat agaggtcatg aagcaaaact agtaggatat
      721 tgcatcgctg atagattagg agccgcatac aaaggtgatg tagaaactgg tctatttttc
      781 tctggttcaa atggatataa aattgataag ataatctctg ttaaagagtt aatggaaaaa
      841 ttaactaaag gagagtagtt attttaacta aattctttat ttgccttcta cttattatca
      901 atatatcttt tgcctctagc ttggaacagc agtacaaaga ggcaagaata gagtttttaa
      961 aatcttcatt aagaaaagat gacaatcaaa aaatagatga tttaaaaaaa attattgaac
     1021 tagggaaaaa gttaaataaa gatgtaagac ctgatgaaaa aaaattagct gttttaaata
     1081 aaagtattaa agtaataaat aacaaaaata tagagtcagc tcaaacttct tcaaataata
     1141 agcaaaataa agagaaacaa tcaaatcttt tatactctat aaaaaatgta actacttcaa
     1201 acaactcaat agtagttgat tttaatgttg atataaaaga agatgatatt aaattttttg
     1261 agctaaaacc ttctccttta tatagagatg tttttgatat aaaaggttat tttaaagatg
     1321 ccttagctac aaaattagca attcagggtg ttgaacaaat tacaataggt cagtttcaac
     1381 ctgatgtttt aagaattgta atttcaggca ataataatcc agaaacatct tataaaataa
     1441 caaaaagaca actaataata gatgtaaata gtaaacaatc aacaaaaaaa gatgaacaaa
     1501 taaaagagat ttcaaaacaa aaaattgaac ctaattcaaa tattcaagaa atggttgatt
     1561 tgaaaaactc tattcgttct atttcatcaa gtggagataa gattactatc cagtttaaca
     1621 aaaaaatatc acaaaaagat ttaaaatatt ctactcttaa gcaaaatgga aattttgaat
     1681 atatttttga tattaatgga aaatatagtt atattgaacc tacaaaacta aatttgaata
     1741 gtatagataa agttattact acagaaaata aaaatagcgt tagaataaga ttaataaata
     1801 aaaataatcc taaaattaaa tatattttaa aatcaaatga gctaactatt gaagttttag
     1861 aaactcaaag tgaaaaagta gaagttgcta aaaacagtag tgattcaaac agtaaaacta
     1921 atcttcctac aaaagtttca aaacaagttc aaaatacaac acccaaaaat gctataaata
     1981 aaacaatagt tatagatgca ggacatgggg gagatgatgt tggtgctgtt ggaccaaata
     2041 aagagtatga aaaagttata aatttagaag tagcaaaata tcttgataat atattaaaac
     2101 aaagaggata caaggtatat ttgactagat caactgataa atttattaaa gttatggata
     2161 gaactatttt agcaaatgag aaaaatgctg acctttttat ctcaatacat acaaactcta
     2221 tgccaaaaga gaaagctagt agtacaagcg gtattgagac attttttcta agtcctgcaa
     2281 gaagtgaaag agcaaaaaaa gttgctgctt tagaaaataa agatgatatt agagagatga
     2341 gtgaaagttc aaaaaatgta tttttagaga gcttaaatcg tccaagaatt acagcatctc
     2401 ataaatttgc aatagatgtt caatcagggc ttttacaagc agctagaaca aaatataaag
     2461 atgtaagaga tacaggtgta aaagagggac cattttgggt tttagttggt gaacaaatgc
     2521 catctattct tattgagcta ggatttatat ctcatcctgt tgaaagtaaa agattatatg
     2581 ataaagatta tcagcaagta cttgcaaatg gtattgctaa tggaatagat tcttactttc
     2641 taaaaaatcc ttaatttctg atactaattt tttttgcatt gactttatct ttggaagctt
     2701 ttttaatgaa aagaatttta cttttgaatt ttcccaagag agatattcac ttttta
//
LOCUS       DAOQAN010000090         2754 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_51589_length_2754_cov_2.192664, whole genome shotgun sequence.
ACCESSION   DAOQAN010000090 DAOQAN010000000
VERSION     DAOQAN010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2754
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51589_length_2754_cov_2.192664"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(101..316)
                     /locus_tag="PKO10_02175"
     CDS             complement(101..316)
                     /locus_tag="PKO10_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW65995.1"
                     /translation="MHTIKLKVGDGIYNHLMFFLKNLKTNELEIIEDKQINTTKDSEI
                     SDISKLSNKSFENIWENKEDEVYDKFL"
     gene            450..2342
                     /gene="ciaB"
                     /locus_tag="PKO10_02180"
     CDS             450..2342
                     /gene="ciaB"
                     /locus_tag="PKO10_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014469027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein CiaB"
                     /protein_id="HNW65996.1"
                     /translation="MTKQNFIDDLQKIYDYLDNEKAKTNELLKFLENKEFDKLFIIDD
                     FAKSLGLQMNDDLRFALVTRLVNLRDDSLVQVLKKLEKSEKEIIEIQEKAYQFVKKYW
                     HEKHKNLIDFIVQNKLLTPFYREIFIGVYNVGLAMSSWQSSWTAHIINKINKELSAKF
                     EGNEEKIMKYLEDENLLDLGHGGIVADRCYSALVKEKETYSSKAYIKAFKKEVTQVVD
                     SLEEFVDKLIELEDEIYNQKWDYIKYIQSLIVAFSEDKTDELVNKWANVDRAWMKITT
                     PIQIGHPLEYYEDHFRKAVALEWDIRVTNPKFAQNDHRVNKIKSAFTKIFNSFEQNAK
                     SEEYKKIFDFSFKSLDKVQLYVGRPALFFGAELNGLFSAQVVPNDEVVSLEEGKKIFA
                     FSDEILQSSRAKPFLKLSREIFGQELLTKDRNFLFKQTASWHSVYDITTIGHEYGHIL
                     WCDEETESFMNKTGNFKNIEEFKATTGGLISYLLDEKDDEKHLKEAILIDLIKRSVGL
                     ISWMEVDEVQPYYCEGLIHLCALFESNILTWNEDKKELKIDLEDEKFEKLKVWYIKNY
                     TALAKHYLEKLDATKFLNIYATKKDKYFMPNDENIKSFVEYYFKRYQEIGQELDTFDK
                     KENYTK"
     gene            complement(2412..>2754)
                     /locus_tag="PKO10_02185"
     CDS             complement(2412..>2754)
                     /locus_tag="PKO10_02185"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HNW65997.1"
                     /translation="TAMQAQIATGKSLEEVFTPYIEQLPKNVYISIDIDGLEPLNCPN
                     TGTPVPGGLRYGELEYLIFMVVKSGRKIIGFDLVEVGDSKNGWDANVGARVLYNLCGA
                     LLASQGKIEYR"
BASE COUNT         1074 a          331 c          420 g          929 t
ORIGIN      
        1 ggatgtttaa tataattaaa ctccaattta taattggtaa agtctgataa attatgatga
       61 aaacaaaaaa tatggaaatt taactactac aatatcgtat ttagagaaat ttatcatata
      121 cttcatcctc tttattttcc caaatatttt caaaagattt atttgaaagt tttgatatat
      181 cagaaatttc actatctttt gtagtgttga tttgtttatc ttcgataatt tctagttcat
      241 ttgttttaag atttttcaaa aaaaacatca agtgattgta aatcccatca cctactttta
      301 attttattgt atgcatctca aactcctgct gttaaaataa ataagattat agcaaattat
      361 atctttttat caattcttat aaaatccaaa taaacttctt taatcctaag ttttgataga
      421 atacaaactt taaaaataag gaacaattta tgacaaaaca aaattttata gatgatttac
      481 aaaaaattta tgactattta gacaatgaaa aagcaaaaac aaatgagtta ttaaaatttt
      541 tagaaaacaa agagtttgat aaactattta taatcgatga ttttgcaaag agtttaggtt
      601 tacaaatgaa tgatgattta agatttgcac ttgttacaag gcttgtaaat ttaagagatg
      661 atagcttagt tcaagttttg aaaaaacttg aaaagagcga aaaagagatt attgaaattc
      721 aagaaaaagc gtatcaattt gtaaaaaaat attggcacga aaaacataaa aacttaatag
      781 attttatagt tcaaaataag cttttaactc ctttttatag agagattttt attggagttt
      841 acaatgttgg tcttgctatg tcatcttggc aaagctcttg gacagcacac ataataaata
      901 aaataaataa agagctaagt gctaaatttg aaggcaatga agagaaaatt atgaaatatc
      961 ttgaagatga aaatcttctt gatttaggac atggtggaat agttgctgat agatgttatt
     1021 cagcacttgt aaaagagaaa gaaacttata gttcaaaagc atatataaaa gctttcaaaa
     1081 aagaggttac acaagttgta gattctcttg aagagtttgt agataaatta attgagcttg
     1141 aagatgaaat ttataatcaa aaatgggatt acataaaata tattcaaagt ttaattgtag
     1201 catttagtga agataaaaca gatgagcttg taaataaatg ggcaaatgta gatagagctt
     1261 ggatgaaaat aactacacct attcaaatag gtcatccact tgaatactat gaagaccact
     1321 ttagaaaagc tgttgcacta gagtgggata ttagagttac aaatccaaaa tttgctcaaa
     1381 atgaccatag agtaaataaa ataaaatcag catttacaaa gatttttaat agttttgagc
     1441 aaaatgcaaa aagtgaagag tacaaaaaaa tatttgattt tagcttcaaa tcacttgata
     1501 aagtacaact ttatgttggt cgaccagcac tgttttttgg agcagagtta aatggtcttt
     1561 tttcagctca agttgtacca aatgatgaag ttgtaagtct tgaagagggt aaaaaaatat
     1621 ttgcattttc tgatgagatt ttacaaagta gtcgtgctaa accattttta aaacttagcc
     1681 gtgagatatt tggacaagag ttattaacaa aagatagaaa ttttcttttt aaacaaacag
     1741 cttcatggca tagtgtttat gatattacaa ctattgggca tgagtatgga catattttat
     1801 ggtgcgatga agagacagag agttttatga ataaaacagg aaattttaaa aatatagaag
     1861 agtttaaagc aacaactggt ggattaatct catatttact tgatgaaaaa gatgatgaga
     1921 aacatctaaa agaagctatt ttgatagatt tgataaaaag aagtgttgga cttatctctt
     1981 ggatggaagt tgatgaagtt caaccatact attgcgaagg cttaattcat ctttgtgctc
     2041 tttttgaaag taatatttta acttggaatg aagataaaaa agagctaaaa attgatttag
     2101 aagatgaaaa atttgaaaaa ctaaaagttt ggtatatcaa aaattatact gctttagcaa
     2161 aacactattt agaaaaacta gatgctacaa agtttttgaa tatttatgct actaaaaaag
     2221 ataaatattt catgccaaat gatgaaaata taaaatcttt tgtagaatat tatttcaaaa
     2281 gatatcaaga gattggtcaa gagttagata cttttgataa aaaagagaat tacacaaaat
     2341 aactatttct atcataacca ctattttaag atggttatga tagtttaaat ttatattatg
     2401 gttttagagc cttatctata ctctatttta ccttgacttg ctaataaagc tccacaaaga
     2461 ttgtataaaa ctctagctcc aacgtttgca tcccaaccat ttttactatc tccaacttca
     2521 actaagtcaa aacctataat ctttcttcca ctttttacta ccataaaaat taaatactct
     2581 aactcgccat atcttaatcc gccaggaact ggagttccag tatttgggca gtttaatggt
     2641 tcaagaccat ctatatcaat agagatataa acattttttg gaagctgctc tatatatgga
     2701 gtaaaaactt cttcgagaga ttttccagtt gcaatttgag cttgcattgc tgta
//
LOCUS       DAOQAN010000091         2723 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_52434_length_2723_cov_2.489130, whole genome shotgun sequence.
ACCESSION   DAOQAN010000091 DAOQAN010000000
VERSION     DAOQAN010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52434_length_2723_cov_2.489130"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(121..852)
                     /gene="tatC"
                     /locus_tag="PKO10_02190"
     CDS             complement(121..852)
                     /gene="tatC"
                     /locus_tag="PKO10_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013135680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HNW65998.1"
                     /translation="MFDDLKPHIADLRKRLVISSITVVIMFFACFSFYEPILEWMMAP
                     VKHALPAGTSMIAVEIQETFFTAMKVAFFGGFIISLPVIFWQLWLFLAPGLYDHEKKL
                     VVPFVFFATLMFLLGASFAYYIVVPVGFDFLIAFGNSVVSVLPSIGKYVGFFTKLLIG
                     FGIAFELPVITFFLAKIGLVNDQMLKDFFRYAVVLIFIVAAVLTPPDVISQVLMAAPL
                     LILYGVSIYIAKVFNPAQKEEEEEE"
     gene            complement(852..1262)
                     /gene="tatB"
                     /locus_tag="PKO10_02195"
     CDS             complement(852..1262)
                     /gene="tatB"
                     /locus_tag="PKO10_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014474291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HNW65999.1"
                     /translation="MFGMGITEILLIAIVAVIALGPEKLPDAMVKIAKMFNSIKKGIS
                     DAKTTLDNELNIAELKEEANKFKAQIEDTKSSLSIENKFDLGLDDILKDDLEASKNDN
                     EVEKIKEKFAKVNEDKAQKSKDEKFSLKNSEEVK"
     gene            complement(1368..2387)
                     /locus_tag="PKO10_02200"
     CDS             complement(1368..2387)
                     /locus_tag="PKO10_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNW66000.1"
                     /translation="MEYRYIGKTGLRVSSICMGTMTFGSSTSKDEAFKILDKAYDRGI
                     NFFDTAELYPVSPKKETIGNTEKIVGEWLKTKPRDSVILATKIAGAASGWFVPPVRHG
                     LTAIDSFHIKKAIEDSLRRLQTDYIDLYQMHWPDTIVPIEESLKAFDALVKEGKVRYI
                     GTSNDSAYGLTKANEISKYKGYARFESIQNNFSLLNPRFHDELANVCRLENISLLPYS
                     PMAGGVLSGKYCGGFYPEGSRFETYLKESNKRVQAMAHRFVNERTLEATKKYLELAKE
                     YEVSPVTLAISYSKHFDFVASTIVGARKIEQLDDSLKAFDFKISNELLKRIEIIQKDI
                     LYPMG"
     gene            2513..2716
                     /locus_tag="PKO10_02205"
     CDS             2513..2716
                     /locus_tag="PKO10_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HNW66001.1"
                     /translation="MIKKKIVDLKDKNLEFIQKDETIKLLSFNTLKMSLEIAIFKNDK
                     FIKNSSMVFAHLPKSLKAKLNPK"
BASE COUNT          984 a          465 c          300 g          974 t
ORIGIN      
        1 tttttctctc tattgtaaac tagcattttt gcactactag gaggatttac tggaaaagta
       61 gcaatttgac ttgcaggaag attaaaatcg taacttgaag tttttaaaac atctatcaaa
      121 ctactcttct tcctcttctt ctttttgcgc tggattaaat acttttgcta tataaataga
      181 tactccataa agaatcaaca aaggagccgc cattaaaact tggctaataa catctggtgg
      241 agttaaaact gcagcaacaa taaatattaa aacaacagca tatctaaaaa aatctttaag
      301 catttgatca tttacaagac caatttttgc aaggaaaaat gtaataacag gtaattcaaa
      361 tgcaatacca aaaccaatca aaagttttgt aaaaaatcca acatattttc caatactagg
      421 taatacacta acaacactat taccaaatgc aattaaaaaa tcaaatccta caggaacaac
      481 tatataataa gcaaaagatg ctcctagtaa aaacattaaa gttgcaaaaa atacaaaagg
      541 aacaacaagt tttttctcat ggtcgtacaa accaggagcc aaaaataacc ataactgcca
      601 aaaaattaca ggtaaagaga taataaatcc tccaaaaaat gcaactttca tagctgtaaa
      661 aaatgtctct tgaatttcaa ctgctatcat tgaagtccca gctggaagag cgtgtttaac
      721 aggagccatc atccactcta aaataggttc ataaaaagag aaacaagcaa aaaacataat
      781 aactacagta atactagaaa taaccaatct ctttctaaga tctgctatat ggggttttaa
      841 atcatcaaac attatttaac ttcctcacta ttttttaaag agaacttttc atctttactt
      901 ttttgagctt tatcttcatt tacttttgca aatttttctt taattttttc aacctcatta
      961 tcattttttg aagcttctaa atcatctttt aaaatatcat ctagacccaa atcaaactta
     1021 ttttcaatac ttaaagatga ttttgtatct tcaatttgag ctttaaattt atttgcctct
     1081 tcttttaatt ctgctatatt tagctcatta tccaaagttg tttttgcatc gcttatccct
     1141 tttttaatac tgttaaacat ttttgctatt tttaccatag catctggcag tttttcggga
     1201 cctaatgcga taactgcaac tattgcgatt agaagaattt cagttattcc cattccaaac
     1261 attttttgct ccattttttt ataaggttgt gattttatca aaatagaaca aagatagtga
     1321 ttaatcaaaa aatctaaact actaaaaaag ttctaattta tctaaattta acccataggg
     1381 tataagatat ctttttgaat tatctcaatt ctttttaata actcatttga aattttaaaa
     1441 tcaaaagctt tcaaagagtc atctagttgc tctatttttc ttgcccctac aattgtagaa
     1501 gctacaaaat caaaatgctt tgaataagaa attgctaaag taacaggact tacttcatac
     1561 tcttttgcaa gttctaaata ttttttagta gcttctagag ttctttcatt tacaaatcta
     1621 tgtgccatag cttgaactct tttattactc tcttttaaat atgtttcaaa tcttgaacct
     1681 tctggataaa atccaccaca atattttcca cttaaaactc cacccgccat tggtgaatat
     1741 ggtaataaag atatgttttc aagcctacaa acatttgcta actcatcatg aaatcttgga
     1801 tttaaaagtg aaaaattatt ttgaatagac tcaaatctag catatccttt atatttactt
     1861 atttcatttg cttttgtaag cccataagca ctatcatttg aagttcctat atatcttact
     1921 tttccctctt ttacaagtgc atcaaaagct tttagactct cctctattgg aacaatagta
     1981 tctggccaat gcatttgata aagatctata taatctgttt gaagtcttct taaactatct
     2041 tcaatagctt tttttatatg aaaactatca attgctgtca aaccatgtct tacaggtgga
     2101 acaaaccaac ctgaagcagc tcctgcaatt tttgttgcta gtattacact atctcttggc
     2161 tttgttttaa gccactctcc tactattttt tcagtatttc caatagtctc ttttttagga
     2221 ctaacaggat atagttctgc cgtatcaaaa aaatttattc ctctatcata agccttatcc
     2281 aatatcttaa atgcttcatc ttttgatgta ctacttccaa aagtcatagt tcccatacaa
     2341 atagaactta ctctaagacc tgtttttcca atatatctat attccatttt tagcctttta
     2401 tatcgaattt tatagattat acaaatctct ttcttaaaaa tataaataaa aaaagaaatt
     2461 tattttaata ctattttagt agaatttgaa aaatattttt taggatttaa ttttgataaa
     2521 aaagaaaata gtagatttaa aagataaaaa tcttgaattt atacaaaaag atgaaacaat
     2581 aaaactacta agttttaata ctttaaaaat gagtttagaa atagctattt ttaaaaatga
     2641 taagtttata aaaaatagct ctatggtttt tgcacatctt ccaaaaagct taaaagcaaa
     2701 actaaatccc aaataaaaag aga
//
LOCUS       DAOQAN010000092         2699 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_53155_length_2699_cov_2.569592, whole genome shotgun sequence.
ACCESSION   DAOQAN010000092 DAOQAN010000000
VERSION     DAOQAN010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2699
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53155_length_2699_cov_2.569592"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="PKO10_02210"
     CDS             <1..505
                     /locus_tag="PKO10_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002952114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HNW66002.1"
                     /translation="RLKSLGVKNIVMLTGDTKTKALKIADELGINEVKAELLPQDKAN
                     IVKELMQEGKKVAFIGDGINDAPALISSHVGISMSRGADIAKATADISLLKDDIAAVV
                     EAKEFANKTMNLINTNFNATVGINSAILLGATFGLFSPIVTAVLHNGTTIGLLLNSIK
                     GVKLAKK"
     gene            680..928
                     /locus_tag="PKO10_02215"
     CDS             680..928
                     /locus_tag="PKO10_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005021861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66003.1"
                     /translation="MKELKFDLELQKEVAKIGMSATLGATVVTSMFMKNKIAKNTHII
                     AGVAFCGFALWHHMLYQPKKSKKENEKNIINPTKSSIK"
     gene            989..1696
                     /gene="pyrH"
                     /locus_tag="PKO10_02220"
     CDS             989..1696
                     /gene="pyrH"
                     /locus_tag="PKO10_02220"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HNW66004.1"
                     /translation="MNKRVLVKFSGEALAGTEGYGIDTKILDYIAEEIKTLVEHGVEV
                     AIVIGGGNIIRGVTAAADGVIKRTSADYMGMLGTVINGVAMQEALEYKGLSARLQTAI
                     KMEEIAEPFIVRKAMRHFEKGRVVIFGAGTGNPYFTTDTGATLRATEIGADLLIKATK
                     VNGVYDKDPMKFADAVKLENLTYDRALEDHIKVMDDTAIALAKDNKLPIAVTNMNEKG
                     NLLRIVQGDYSKCSIVK"
     gene            1728..1946
                     /locus_tag="PKO10_02225"
     CDS             1728..1946
                     /locus_tag="PKO10_02225"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014473502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HNW66005.1"
                     /translation="MERLEERISRALKQVDNDRYVLAIAVGQRADELSKGAKPLLSQN
                     TQKMKYTDIAIDEIASGLLKINGFTDKK"
     gene            1967..>2699
                     /locus_tag="PKO10_02230"
     CDS             1967..>2699
                     /locus_tag="PKO10_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNW66006.1"
                     /translation="MNLFFKELQLVNTIEGAIKELEKQIDISPKLNEIIDFIIEAHEG
                     QFRKSGEPYSVHPILVATITSTFSKDEDVIATALLHDVVEDTPFTLEFVEEKWGKNVS
                     NMVKGLTKVADIREENFITSKDSSDTKIVQAALTFRKMLIASIDDPRVLIVKLCDRLH
                     NMLTLAVLPQHKQRRIAEETLVVYVPIANRLGISTLKNELEDLAFFYIYPDEYKKIDD
                     FLKENEQAMQLSFNTFIAITKNLLEK"
BASE COUNT         1073 a          316 c          480 g          830 t
ORIGIN      
        1 aagattaaaa tctcttggtg taaaaaatat tgtaatgcta acaggagata caaaaacaaa
       61 agctttaaaa attgctgatg agttaggtat taatgaagta aaagccgaac ttttacctca
      121 agataaagca aatattgtaa aagagttgat gcaagagggt aaaaaagttg ctttcattgg
      181 agatggtata aatgatgccc ctgctcttat atcttctcat gttggaattt caatgagtcg
      241 tggagctgat atagcaaaag caactgctga tattagtcta ttaaaagatg atatcgctgc
      301 tgttgttgaa gcaaaggagt ttgcaaataa aaccatgaat ttgataaata ctaacttcaa
      361 tgcaactgtt ggaataaata gtgcaattct tttaggtgca acttttggtc tattttctcc
      421 tattgtaaca gctgtattgc acaatggaac aacaatagga ttactattaa attcgataaa
      481 aggggtaaaa ttagctaaaa aatagatttt aataatagtt atcagttttg taagaaatta
      541 aacaatacaa ttcttttaat ttattaaagg atatatttta tgccattttt attaccatta
      601 gcgactttaa tcacaggtgt tttgacagtt aaaactgtaa ataaaactat aaaaacttat
      661 gaaagaagaa aaaagagaga tgaaagagct aaaatttgat ttagaacttc aaaaagaagt
      721 tgcaaaaatt ggaatgagtg caactttagg tgcaactgtt gtaacttcaa tgtttatgaa
      781 aaataaaatt gccaaaaata ctcatattat tgcaggtgtt gcattttgcg gttttgcact
      841 atggcatcat atgctttacc aaccaaaaaa atcaaaaaaa gagaatgaaa aaaacattat
      901 aaatccaaca aaatcttcaa taaaataaac taagtttttg ttaatcatat tttagataaa
      961 atgcaaaaat tattgaagga taaaatagat gaataaaaga gttcttgtaa agttctcagg
     1021 agaagcatta gctggaactg aaggatatgg aattgataca aaaatattag attatatagc
     1081 tgaagagatt aaaactttag ttgagcatgg tgttgaagtt gctattgtta ttggtggtgg
     1141 aaatattatt cgtggtgtta ctgctgctgc tgatggtgtt attaaaagaa caagtgctga
     1201 ttatatggga atgctaggaa ctgtaatcaa tggtgttgct atgcaagaag ctttagaata
     1261 caaaggtttg agcgcaaggt tgcaaactgc tataaaaatg gaagagatag ctgaaccttt
     1321 tattgtaaga aaagctatga gacattttga aaaaggaaga gttgtaattt ttggagcagg
     1381 aactggaaat ccatatttta ctactgatac aggtgcaaca ctaagagcta ctgaaattgg
     1441 tgcagattta cttataaaag caacaaaagt taatggagtt tatgataaag atccaatgaa
     1501 atttgcagat gcagtaaaat tagaaaatct tacatatgat agagctttag aagaccatat
     1561 aaaagttatg gatgacactg ctattgcttt agcaaaagat aataaactac caattgcagt
     1621 tacaaatatg aatgaaaaag gaaatttgct aagaattgta caaggcgatt atagcaaatg
     1681 ttcaatagtt aaatagttca aaatagattt taaaaaggat attaaaaatg gaaagattag
     1741 aagagagaat ttcaagagct ttaaaacaag ttgacaatga tagatatgtt ttagcaattg
     1801 cagttggaca aagagcagat gaattaagca aaggtgcaaa accactttta agccaaaata
     1861 ctcaaaaaat gaaatataca gatattgcaa ttgatgaaat agcaagtgga ttgctaaaaa
     1921 tcaacggttt tactgataaa aaatagatct ctacttagta gattttatga atttattctt
     1981 caaagagcta caactagtaa atacaattga aggggcaata aaagagcttg aaaagcaaat
     2041 tgatattagc cctaaactaa atgagattat tgattttatt atcgaagctc atgagggtca
     2101 atttagaaaa agtggtgaac cctattcggt tcatccaatt cttgtagcaa caataactag
     2161 tactttttca aaagatgaag atgttattgc aactgcactt cttcatgatg ttgtagaaga
     2221 tacacctttt acattagaat ttgttgaaga aaaatggggc aaaaatgtct ctaatatggt
     2281 taaaggtctt acaaaagtag cagatattag agaagaaaat tttattactt caaaagattc
     2341 aagcgataca aaaatagttc aagcagcact tacatttaga aaaatgttaa tagcatcaat
     2401 agatgatcca cgagttttaa tagtaaaact ttgtgataga cttcataata tgcttactct
     2461 tgctgtttta ccacaacata aacaaagaag aatagctgaa gagactctgg ttgtttatgt
     2521 tccaattgca aacagacttg gtatttcaac acttaaaaat gagttagaag atttagcatt
     2581 tttctatatt tatccagatg agtacaaaaa aattgatgat tttttaaaag agaatgaaca
     2641 agctatgcag cttagtttca atacttttat tgctattaca aaaaatttat tagaaaaaa
//
LOCUS       DAOQAN010000093         2691 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_53361_length_2691_cov_2.845220, whole genome shotgun sequence.
ACCESSION   DAOQAN010000093 DAOQAN010000000
VERSION     DAOQAN010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53361_length_2691_cov_2.845220"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="PKO10_02235"
     CDS             <1..454
                     /locus_tag="PKO10_02235"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66007.1"
                     /translation="TTISYELLKENFSKNNIIARVIFSKLLIVLIIVLATLFYIPNYT
                     ILQTVAIAFTISAATLFPTLVLGIFYKKTNTIGAIFGVISSLFFTLSYCIYFLYINSS
                     SNYFFGISSEAIGSIGAILNILITVIVSKLTSKQKNINESIDFTQHKE"
     gene            457..2274
                     /locus_tag="PKO10_02240"
     CDS             457..2274
                     /locus_tag="PKO10_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative nucleotidyltransferase substrate
                     binding domain-containing protein"
                     /protein_id="HNW66008.1"
                     /translation="MSIQDQESFLSKTHPFEVLTPSQIATCIEHMDIAYYPKDTVLIS
                     PEKIPKYFFIIIKGSVFEYSPENVVVMDYQSEDTFDSNSLIYGKCENSFRVNEELICY
                     EIEKKTFLELIDKNQKFKDFFLKDLVNKIRTLKDKENTSQLSSFMIAKVSDTLIHEAC
                     IVKKDTKLIDAIDKSMKYKTSTIIIEGDNKEYGIITDSLLKVKVLLEGRDLSIPVSDI
                     AIFPLLTIKQDDYLFEALTILVKRNIKRVGVVDNNGNMVGILEQIDILSHFANHTYVI
                     ESKIKNAKNIEDLKDASKDFIDIITSLQAKGVKLHHISNLIGQLNTKVYQKLFSLILP
                     LELQKDACLFVMGSEGRNEQIIKTDQDNALIVRDGIDVEKYRPYMQKITDALIFLGYP
                     PCEGNIMVSNPAWCKSYLNFKDDISRWVGTQDMKNFLDLAIFADSFAVAGDKQMLIDL
                     KEFLYSKVQNDIFMAYFAKSTTAFETPTTISTFMGKENFINIKKAAIFPIVHGIRSLA
                     LKEKIKETTTIKRIQILEQRKILEKDRAAELLEALEIVNTLRLRHQIDLLQQNKPLSN
                     EIDTNSLGKIERDLLKESFKIVNDFKKIVSYIFKLDKIY"
     gene            2276..>2691
                     /locus_tag="PKO10_02245"
     CDS             2276..>2691
                     /locus_tag="PKO10_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HNW66009.1"
                     /translation="MFKNIIKIWQRKKLLDKRFDFLFNEPPQDEYISLDCETTGLNPK
                     KDEILSIGAVLIKDNKILMRKTFNIFVKPSQNINPESIKIHLLREIDLQNAIEPDVAI
                     YKLLDFIGSRPIVGYYIDFDIEMISNAINSFLNSDIA"
BASE COUNT         1105 a          299 c          371 g          916 t
ORIGIN      
        1 aactacaata tcttatgaat tgctaaaaga aaattttagt aaaaataata ttattgctag
       61 agttatattt tcaaaattat taatagtttt aattatagtt ctagcaactt tattttatat
      121 tccaaactat acaattttgc aaacagttgc tatagctttt acaatatctg cagcaacact
      181 atttccaact ttagttttag gaatttttta taaaaaaaca aatacaattg gggcaatatt
      241 tggtgtaata tcaagcctat tttttacact atcttattgt atctattttt tatacataaa
      301 tagttcaagc aattactttt ttggtattag tagtgaagca attggctcta ttggtgcaat
      361 tttaaatatt ttaattacag ttatagtttc aaaactaaca tcaaaacaaa aaaatataaa
      421 tgaatctatt gattttacgc aacacaagga gtaaaaatga gtattcaaga tcaagagagt
      481 tttttatcaa aaactcaccc atttgaggtt ttaacaccaa gccaaattgc tacatgtatt
      541 gagcatatgg atatagctta ctatccaaaa gatacagttt taattagtcc agaaaaaata
      601 ccaaaatatt tttttattat tatcaaaggt tcagtttttg aatactctcc tgaaaatgtc
      661 gtagtaatgg attatcaaag cgaagatact tttgattcaa attctctaat ttatggaaaa
      721 tgtgaaaata gttttagagt aaacgaagag ctaatatgtt atgaaataga gaaaaaaact
      781 tttttagagt taattgataa aaaccaaaaa tttaaagatt ttttcttaaa agatttagta
      841 aataaaatca gaacattaaa agacaaagag aatacttctc aattatcatc ttttatgata
      901 gcaaaagtaa gtgatacttt aatacatgaa gcttgtatag taaaaaaaga tacaaaatta
      961 atagatgcaa tagataaatc aatgaaatac aaaacttcaa ctattataat tgaaggtgat
     1021 aataaagagt atggaattat tacagattct ttgctaaaag taaaagttct tttagaagga
     1081 agagatttat ccattcctgt tagtgatatt gctatttttc cacttttgac tattaaacaa
     1141 gatgattatc tatttgaagc tttaacaatt ttagtaaaaa gaaatattaa aagagttgga
     1201 gtagttgata ataatggaaa tatggttgga attctagaac aaatagatat tttatctcat
     1261 tttgcaaatc atacatatgt tattgaatca aagattaaaa atgcaaaaaa tattgaagat
     1321 ttaaaagatg ctagtaaaga ttttatagac ataattacaa gtcttcaagc aaaaggtgtt
     1381 aaattacacc atatttcaaa tcttattggg caattaaaca caaaggttta tcaaaaatta
     1441 ttctcattaa ttcttccttt agagttgcaa aaagatgctt gtctatttgt aatgggaagt
     1501 gaaggaagaa atgaacaaat tataaaaaca gatcaggata atgctttgat tgtaagagat
     1561 ggtatcgatg ttgaaaaata cagaccttat atgcaaaaaa ttacagatgc tcttatattt
     1621 ttaggttatc ctccttgtga aggaaatata atggtttcta atcctgcttg gtgtaagtca
     1681 tatctcaatt ttaaagatga tatatcaaga tgggtaggta ctcaggatat gaaaaacttc
     1741 ttagatttag caatatttgc tgactcattt gcagttgcag gtgataagca gatgctgatt
     1801 gatttaaaag agttcttata ctcaaaagta caaaatgata tttttatggc atattttgca
     1861 aaatcaacaa cagcatttga aactccaaca acaatatcta cttttatggg taaagaaaat
     1921 tttataaata ttaaaaaagc tgcaattttt ccaatagttc atggaattag aagtttagcc
     1981 ttaaaagaga aaataaaaga gacaacaact ataaaaagaa tccaaatttt agagcaaaga
     2041 aaaattttag aaaaagatag agccgcagag ctattagaag cactagaaat agtaaatact
     2101 ttaagattga gacatcaaat agatttatta cagcaaaata aaccactatc aaatgagata
     2161 gataccaata gtttaggaaa aatagagaga gatttattaa aagaatcatt taaaattgta
     2221 aatgacttca aaaaaattgt aagttatata tttaaactag ataagattta ttaaaatgtt
     2281 taaaaatatt ataaaaattt ggcaaagaaa aaaactttta gataaaagat ttgatttttt
     2341 atttaatgaa cctccacaag atgaatatat aagtttagac tgtgaaacta caggtttaaa
     2401 tcctaaaaaa gatgaaattt tatctattgg tgcagtctta atcaaagata acaaaatttt
     2461 aatgagaaaa acatttaata tatttgtaaa accatctcaa aatataaatc ctgaatctat
     2521 aaaaattcat ctcttaagag aaatagattt acaaaatgct attgaacctg atgttgctat
     2581 atataaactt cttgatttta ttggttcaag accaatagtt ggctactata tagattttga
     2641 tatcgaaatg atttcaaatg caattaatag ttttttaaat tctgatattg c
//
LOCUS       DAOQAN010000094         2681 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_53697_length_2681_cov_2.919650, whole genome shotgun sequence.
ACCESSION   DAOQAN010000094 DAOQAN010000000
VERSION     DAOQAN010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53697_length_2681_cov_2.919650"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="PKO10_02250"
     CDS             complement(<1..512)
                     /locus_tag="PKO10_02250"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014474779.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HNW66010.1"
                     /translation="MKGKIIQVMGPVVDVEFDGYLPEINEAIDVSGIASGKDRLVLEV
                     AAHIGDGRVRAIAMDMTDGLIRGQECIATGGPIKVPVGEAVLGRIFNVIGDPVDEGAP
                     IPADVERWSIHRSAPAFEEQSTKTEMFETGIKVVDLLAPYSKGGKVGLFGGAGVGKTV
                     IIMELIHNVA"
     gene            complement(534..1418)
                     /gene="atpG"
                     /locus_tag="PKO10_02255"
     CDS             complement(534..1418)
                     /gene="atpG"
                     /locus_tag="PKO10_02255"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HNW66011.1"
                     /translation="MANLKEIKIKINSVKNTQKTTKAMKLVSSAKLTRTRQLSEQSRS
                     YANKINEVLSDIATRVSKVQDDGNIGRAFIQNSTPKTVDIVFVTADKGLCGGFNVATI
                     KTVSKLINEYEAKGAKVRLRAAGKKGVEFFSFQGATLEQKAVELSSAPTYEKASNYIK
                     LAVEDFKNELTDKVIIVYNGFLNMLTQEIRVKEILPVSLEKVEISETTSMLNIEPDDD
                     DEVLKELTDKYIDFNMYYALIDSLAAEHSARMQAMEAASKNAKEKVNSLTVEYNKARQ
                     AAITTELIEIISGVEALK"
     gene            complement(1429..>2681)
                     /gene="atpA"
                     /locus_tag="PKO10_02260"
     CDS             complement(1429..>2681)
                     /gene="atpA"
                     /locus_tag="PKO10_02260"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HNW66012.1"
                     /translation="CKRLGELLEVPVGEALVGRVVNALGEPIDGKGAINSTETRYVEE
                     KAPGIMSRKSVHEPLQTGIKAIDALVPIGRGQRELIIGDRQTGKTTVAIDTILNQKGE
                     NVICIYVAIGQKSSSVASVVRTLEEAGAMDYTIVVNASASDSSALQFLAPYTGVTIGE
                     FFRDNGKHALIIYDDLSKHAVAYREMSLILRRPPGREAYPGDVFYLHSRLLERAAKMS
                     DEKGAGSMTALPIIETQAGDVAAYIPTNVISITDGQIFLETNLFNSGIRPAINVGLSV
                     SRVGGAAQIKATKQVAGTLKLSLAQYRELEAFAQFASDLDEATRRELELGQRMVEVLK
                     QGVNKPLVIEKQVVIIYAGTKGYLNDVAVKDVVRFENELHAFIEKKYSNILDAIKSSQ
                     KIDDNTEAQLKAALEEFKTVFNAN"
BASE COUNT          802 a          544 c          349 g          986 t
ORIGIN      
        1 aatgcaacgt tatggattaa ttccataata ataactgttt ttccaactcc agcaccacca
       61 aatagtccaa cttttccacc ttttgaatat ggtgctaaaa ggtcaacaac tttgatacct
      121 gtttcaaaca tctctgtttt tgttgattgc tcttcaaaag caggagcaga tctatgaata
      181 gaccatcttt caacatctgc aggaatagga gcaccttcgt caactggatc accaataacg
      241 ttaaaaattc ttcctaaaac cgcttcacca actggaactt taataggtcc accagtagct
      301 atacactcct gacctcttat taatccgtct gtcatatcca tagcgatagc tctaactctt
      361 ccatcaccaa tatgcgctgc tacttctaat actaatctat ctttacctga tgcaatacca
      421 gatacatcaa tagcttcgtt aatttctggt aagtatccat cgaactctac gtcaacgact
      481 ggacccatta cctgaataat tttacctttc atatagggct tctccttatt aaattatttt
      541 aatgcttcaa caccactaat aatctctatt agctctgttg taattgctgc ttgtctagct
      601 ttattgtatt caactgttaa actatttact ttctcttttg catttttact tgcagcttcc
      661 atagcctgca ttctagcact gtgttcagct gctaatgagt ctataagtgc ataatacata
      721 ttgaaatcaa tatatttatc agttaactct tttaaaactt catcatcatc atctggttct
      781 atatttagca tagaagtagt ttcacttatt tcaacttttt ctaaactaac tggcaatatc
      841 tcttttactc taatttcttg agttagcata tttaaaaaac cattataaac aattattact
      901 ttatcagtaa gctcattttt aaaatcttca acagcaagtt ttatgtaatt agatgctttt
      961 tcataagttg gagctgatga taactcaaca gctttttgct caagagtagc accttgaaat
     1021 gagaagaatt caactccttt ttttccagca gctcttaatc ttacttttgc accttttgct
     1081 tcgtattcat ttattaactt actaacagtt ttaattgttg ccacattaaa accaccacaa
     1141 agtcctttat cggcagttac aaaaacaata tcaactgttt ttggagttga gttttgaata
     1201 aatgctctac caatatttcc atcatcttga actttgctaa ctcgtgttgc aatatcagaa
     1261 agtacttcat ttatcttatt tgcataactt cttgattgct cagacaattg tctagttctc
     1321 gtaagttttg cagaagatac aagcttcata gcttttgtag ttttctgagt atttttaaca
     1381 ctatttattt ttatttttat ctcttttaag ttagccatag actagtcctt agtttgcatt
     1441 aaatacagtt ttaaactctt ctaatgcagc ttttaattgt gcctctgtat tatcatcaat
     1501 tttttgactt gacttaattg catctaaaat atttgaatat tttttctcaa taaatgcatg
     1561 taattcattt tcaaatctta caacatcttt aaccgcaaca tcatttaagt accctttagt
     1621 acctgcataa ataattacaa cttgtttttc aataacaagt ggtttattaa caccttgttt
     1681 taaaacttca accattcttt gtccaagctc taactctctt cttgttgctt catctagatc
     1741 tgatgcgaat tgtgcaaacg cttcaagttc tctatattgt gcaagagata gttttaatgt
     1801 accagcaact tgttttgtag ctttaatttg agcagctcct ccaactcttg aaactgataa
     1861 accaacgtta attgcaggtc taatacctga gttaaatagg tttgtttcta agaatatttg
     1921 tccatcagta atagaaatta cgtttgttgg aatatatgca gcaacgtctc cagcttgagt
     1981 ttcaataatt ggtaacgcag tcatagaacc agcacctttt tcatcactca tttttgcagc
     2041 tctctcaagt aatcttgagt gtaggtagaa tacgtctcct gggtatgctt ctcttcctgg
     2101 aggtcttctt aaaattaatg acatttctct atatgcaact gcatgtttag ataaatcatc
     2161 ataaataatc aaagcgtgtt ttccattatc tctaaagaat tcaccaattg taacaccagt
     2221 atatggagct aagaattgta atgctgatga atcagaagct gatgcattta caacaattgt
     2281 ataatccata gctccagctt cttcaagtgt tcttacaact gaagcaacag aagatgattt
     2341 ttgtccaatt gcaacataaa tacaaataac attttcacct ttttggttaa gaattgtatc
     2401 aatagcaaca gttgtcttac cagtttgtct atcaccaata ataagctctc tttgacctct
     2461 tccaattgga actaatgcat caatagcttt aataccagtt tgtaatggct catgaacaga
     2521 ttttcttgac ataattccag gagctttttc ttcaacatat cttgtttcag ttgaattgat
     2581 tgcaccttta ccatcaattg gctcaccaag agcattaaca actctaccaa ctagtgcttc
     2641 accaactgga acctcaagaa gttctcctaa tcttttacaa g
//
LOCUS       DAOQAN010000095         2670 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_54055_length_2670_cov_2.890631, whole genome shotgun sequence.
ACCESSION   DAOQAN010000095 DAOQAN010000000
VERSION     DAOQAN010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54055_length_2670_cov_2.890631"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            155..346
                     /locus_tag="PKO10_02265"
     CDS             155..346
                     /locus_tag="PKO10_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66013.1"
                     /translation="MNSSINLGDLYIIDFWSSDDLGARTTFYDYIDEEFEEDDDKDWI
                     KLNNNVFIDIEGIPYSIFI"
     gene            complement(377..1858)
                     /gene="pyk"
                     /locus_tag="PKO10_02270"
     CDS             complement(377..1858)
                     /gene="pyk"
                     /locus_tag="PKO10_02270"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNW66014.1"
                     /translation="MDKKTKILATLGPSSNSLEMIEKLIDAGANMFRLNFSHGSHEYH
                     QETLDNIRQAMQNKKKIVGILQDISGPKIRVGELKEPFLLEAGDTVTFEKEEVIGYKK
                     GPKEYVVSINYPYILEKIKIDEYIYLYDGIIRAKVIETNPKVKAEIENSGTLSSRKGV
                     NFPNTIIDIEVITKKDEADIAWGVKNRVDYFAISFEQNAKDMRRARELLGDYKGKLIA
                     KIEKFDAVSNIDEIIEVSDGIMVARGDLGIEVPYYDVPTIQKMLIKKSNAKGIPVITA
                     TQMLLSMTQNERATRAEISDVANAVLDGTDIVMLSEESAVGENPVNVVETMHNIIKQT
                     EKIFDHEKRYKFTYLDEFDVIQATVTKLADDLSADGILSLTSSGNSARKMSRYRPITP
                     LYTFAHDKETLTGLTALWGVQPITTVKEAQASKMIQRMLRSLEKREILNKKGLYICTV
                     GYPVGIPGSTNTIKILTPSEIEFYLNFKENREKIKTEKKVNLA"
     gene            1983..2183
                     /gene="thiS"
                     /locus_tag="PKO10_02275"
     CDS             1983..2183
                     /gene="thiS"
                     /locus_tag="PKO10_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HNW66015.1"
                     /translation="MKVIINGQDKILEDNLSLKEIIQNLNIEDKVMACAVNMDIVKKE
                     SWSSYIPKDNDTIELLNFVGGG"
     gene            2187..2519
                     /locus_tag="PKO10_02280"
     CDS             2187..2519
                     /locus_tag="PKO10_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HNW66016.1"
                     /translation="MSREKGDFAEKRAISFLIDLNYIIVETNFYAKKLGEIDIVAKKD
                     NIYHFCEVKSANSFEAAVQNLTKSKLSKIKRSVDYYLQIKKLDVAFCIDAIIVNDDSI
                     EILENITM"
BASE COUNT          894 a          359 c          349 g         1068 t
ORIGIN      
        1 tattcaattt tactcctgaa aagataattt tactcaaaat tagtttaaat tagtaatttt
       61 taaaataatt gctaattttg ccacttcatt gacatttgtg ttatatataa ttattttgcc
      121 gaaaaggtta ataaaaacga acaaggaaat tactttgaat agttctatta atttaggaga
      181 tttatatata atagatttct ggtcatcaga tgatttaggg gcaagaacta ctttttatga
      241 ttatatcgat gaagagtttg aagaggatga tgacaaggac tggataaagt tgaataataa
      301 tgtttttatt gatatagaag gcatacctta ttctatattc atctaattta aaaagagagg
      361 taagcctctc ttttttttat gctaaattta cttttttttc agttttgatt ttttctctat
      421 tttctttaaa gtttaagtaa aactctattt cactaggagt taaaattttt atagtattcg
      481 tacttcctgg aatcccaaca ggataaccaa ctgtacatat atataatcct tttttattta
      541 aaatttctct tttttctaag cttcttaaca ttctttgaat catttttgaa gcttgagcct
      601 cttttacagt tgtaattggt tgaactcccc aaagagcagt tagacctgta agtgtttctt
      661 tatcatgagc aaatgtgtat aatggagtta ttggtctata tcttgacatc tttctagcag
      721 aatttccact acttgtaagt gataaaatac catctgcact taaatcatct gcaagttttg
      781 taacagttgc ttgaattaca tcgaactcat caagatatgt aaatttatat cttttttcat
      841 ggtcaaatat tttttctgtt tgttttatga tattgtgcat agtctcaaca acatttacag
      901 gattttctcc aactgcactc tcttcactta acattacaat atcagttcca tctaaaacag
      961 catttgcaac gtcagatatt tcagctcttg ttgctctttc attttgcgtc attgataaaa
     1021 gcatttgagt tgcagtaatt acaggaattc cttttgcatt tgattttttt attaacattt
     1081 tttgaattgt aggaacatca taatatggaa cttcaattcc taaatcacct cgtgctacca
     1141 ttattccatc acttacttca ataatttcat caatatttga aacagcatca aatttttcaa
     1201 tttttgctat caatttacct ttataatcac ctaaaagttc tcttgctctt ctcatatctt
     1261 ttgcattttg ttcaaatgat attgcaaaat agtcaactct attttttaca ccccatgcaa
     1321 tatcagcttc atcttttttt gtaataactt caatatcgat aattgtattt ggaaaattaa
     1381 ctccttttct tgaacttaat gttccactat tttcaatttc agcttttact tttggatttg
     1441 tttctataac ttttgctcta ataattccgt cgtataaata gatgtattca tcaattttta
     1501 ttttttctaa aatatatgga taatttatag atacaacata ctctttaggt ccttttttat
     1561 atccaataac ctcttctttt tcaaaagtta cagtgtcacc agcttccaat aaaaaaggtt
     1621 cttttaactc accaactctt atttttggtc ctgaaatatc ttgtaaaatt cctacgattt
     1681 ttttcttatt ttgcatagct tgtctaatat tatctagagt ttcttgatgg tattcatggc
     1741 taccatgtga aaaatttaat ctaaacatat ttgcaccagc atcaattaat ttctctatca
     1801 tttccaaact gttactagaa ggtcctagtg ttgctaaaat ttttgttttt ttatccataa
     1861 aatatctcct attttgattt attattatat taaattattg attacttttt aattacaaat
     1921 tactataaaa tagatataag atgaatatta gtataatttg ggataatatt attaggaaga
     1981 agatgaaagt tattataaat gggcaagata aaattttaga ggataattta agcctaaaag
     2041 agattataca aaatctcaat attgaagata aagttatggc ctgtgctgta aatatggata
     2101 ttgttaaaaa agagagttgg agtagttata taccaaaaga taacgacaca atagaacttt
     2161 taaattttgt tggtggtgga tgatttatga gtcgagaaaa aggagatttt gctgaaaaaa
     2221 gagcaatatc ttttttaatt gacttaaatt atataatagt agagacaaat ttttatgcta
     2281 aaaagctagg agagattgat attgttgcaa aaaaagataa tatttatcat ttttgtgaag
     2341 taaaatctgc taacagtttt gaggcagcag ttcaaaattt gacaaaatct aagttatcaa
     2401 aaataaaaag aagtgtagat tattatttac aaataaaaaa attagatgta gcattttgta
     2461 ttgatgcaat tattgtaaat gatgattcaa ttgaaatttt ggaaaatatt actatgtaga
     2521 aagaccaaag gtgaaggtcg cagaaacacc tttggtttta ttgtatatac ttttgtagta
     2581 caaaagaatt ataatttatt ttaatagcag taaactagca ttttataaaa atattttcaa
     2641 aatttacttt taggctattt ttatatatct
//
LOCUS       DAOQAN010000096         2662 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_54306_length_2662_cov_2.383583, whole genome shotgun sequence.
ACCESSION   DAOQAN010000096 DAOQAN010000000
VERSION     DAOQAN010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54306_length_2662_cov_2.383583"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="PKO10_02285"
     CDS             <1..216
                     /locus_tag="PKO10_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66017.1"
                     /translation="INLDYSPAFLLYDDKKLISTIDALLFPFHFKEVLRYVSGKHYVQ
                     YPKTYLDYLRVRQDELLKQGIDINVAE"
     gene            complement(240..842)
                     /gene="coaE"
                     /locus_tag="PKO10_02290"
     CDS             complement(240..842)
                     /gene="coaE"
                     /locus_tag="PKO10_02290"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013134037.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HNW66018.1"
                     /translation="MKQKKEDSFKNAIALTGGIATGKSTVCNLLKLHGFLIIDADKIA
                     HKLLDENSQKIEELFGKQYVENGKVLRKELGKIIFSNEENKLKLEALLHPLIFQEIEK
                     EAKLFEEQNKPYIVDIPLFFEKMHYPISKSIVIYTPKDLQIKRLQNRDNISKEEAMLK
                     ISNQMDIEEKKSLGTYIIDNSKDLKHLQNEVENLISKVLN"
     gene            complement(844..1392)
                     /locus_tag="PKO10_02295"
     CDS             complement(844..1392)
                     /locus_tag="PKO10_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HNW66019.1"
                     /translation="MKDNITFSEMMVHIPLCTHKLAKDILVVSEQNSDLINELDRHKK
                     ESNYKFIELNDLEKIENKSYDVVILPNTKLDIKIVGKLFDILKDDGLIAFSSKVFSRD
                     DNRLIDDLKLVGEKFWIAMPYRFGHQASIIASKKYHPTADLNLQRADFLDDLEYYSSE
                     IHIASFVFPAKQHKDLTGIAKR"
     gene            1489..2484
                     /gene="purM"
                     /locus_tag="PKO10_02300"
     CDS             1489..2484
                     /gene="purM"
                     /locus_tag="PKO10_02300"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HNW66020.1"
                     /translation="MATVSYKDAGVDIDAGNQFVENIKPYVKSTMIPGVLGGIGSFAG
                     AFELPSGYKKPVILAGTDGVGTKLKLAIDAKKFDTVGIDLVAMCTNDLLCNFGEPLFF
                     LDYYATAKLEVEEATQVVKGIAEGCIRSECALIGGETAEMPGMYKEGDFDLAGFCVGI
                     AEKDELDRVERVSAGDVLIALPSSGVHSNGFSLVRKLLLEKLGMSLEDDFQGKKLKDV
                     LLEPTRIYVKEFKANKDKINALAHITGGGITENLPRVLPENLTAVVNRSKIKVLPIFE
                     FMSKHVELEEMYRTFNMGVGMILVVNPANVDAILASTDGYVIGELKSGERKVEFI"
BASE COUNT          891 a          349 c          416 g         1006 t
ORIGIN      
        1 ataaatttag attatagtcc agcttttttg ctttatgatg ataaaaagtt aatctcaaca
       61 atagatgctt tacttttccc ttttcacttc aaagaggttt taagatatgt tagtgggaaa
      121 cactatgtac agtatccaaa aacttacttg gattatttaa gagttagaca agatgaactt
      181 ttaaaacaag gcatagatat aaatgtagca gagtaaaaat tagctcctaa gtaaaggagc
      241 taatttaaaa cctttgatat caaattttct acttcatttt gaagatgttt taaatctttt
      301 gagttatcta ttatatatgt acccaagctt tttttctctt caatatccat ctgatttgaa
      361 attttcaaca tagcttcttc tttgcttata ttatctctat tttgcaatct ttttatttgc
      421 aaatcttttg gagtataaat aacaattgat ttagaaatag ggtagtgcat cttttcaaaa
      481 aatagaggaa tatcaacaat ataaggttta ttttgttctt caaagagttt tgcctctttt
      541 tcgatttcct gaaaaattag tggatgaagt agtgcttcaa gttttagttt attctcttca
      601 ttagaaaata ttattttacc caactctttt cttaaaactt ttccattttc aacatattgt
      661 tttccaaaaa gttcttctat tttttgacta ttttcatcta atagtttatg agctatttta
      721 tcagcatcta ttattaaaaa accatgaagt tttagaaggt tgcaaactgt acttttgcca
      781 gttgcaattc ctcctgtaag tgcaattgca tttttaaaac tatcttcttt tttttgtttc
      841 atactatctt tttgcaatac ctgttaaatc tttatgttgt tttgctggga aaacaaaaga
      901 agctatatga atttcacttg aataatactc taaatcatca agaaaatctg ctctttgaag
      961 atttaaatca gccgttgggt gatatttttt tgaagcaatt attgaagctt ggtgtccaaa
     1021 tctgtaaggc attgctatcc agaatttttc acctactagt tttaaatcat caattaatct
     1081 attatcatct cttgaaaaaa cttttgaaga aaatgcaata agcccatcat cttttaaaat
     1141 atcaaaaagc tttcctacaa tttttatatc tagttttgta tttggtaaaa ttactacatc
     1201 gtaagatttg ttttcaattt tttctaaatc gtttagttca ataaatttgt aattagactc
     1261 ttttttatgt ctatcaagct catttattaa atcactattt tgttctgata ctaccaaaat
     1321 atctttagca agtttatgtg tgcaaagtgg tatatgtacc atcatttcac taaatgtaat
     1381 attatctttc atttttcaat tccttaaaat ttataaatag tctgctataa taccgaaaaa
     1441 tttttaataa atcttaagat ttgagaatat tacgaaaaaa ggtaaaaaat ggcaacagtt
     1501 agttacaaag atgcaggagt tgatattgat gcaggaaatc agtttgtaga aaatatcaaa
     1561 ccttatgtaa aatctacaat gattcctgga gttcttggag gaattggctc ttttgctggt
     1621 gcttttgaac ttccaagtgg ttacaaaaaa cctgttattt tagctggaac agatggtgtt
     1681 ggaacaaaat taaaattagc aattgatgct aaaaaatttg acactgttgg tattgattta
     1741 gttgctatgt gtacaaatga tctgctttgt aactttggag agccactatt tttccttgat
     1801 tattatgcaa ctgcaaaact tgaagttgaa gaagcaactc aagttgtaaa aggaattgct
     1861 gaaggttgta ttagaagtga atgtgcacta ataggtggag agactgctga aatgccaggt
     1921 atgtataaag agggtgattt tgatttagct ggtttttgtg taggaatagc tgaaaaagat
     1981 gagctagata gagttgaaag agttagtgct ggagatgttt taattgcact tccaagttca
     2041 ggagttcact caaatggatt ttcactggta cgaaaacttc ttttagaaaa acttggtatg
     2101 agtttagagg atgattttca aggtaaaaaa ttaaaagatg ttttacttga acctacaaga
     2161 atttatgtaa aagagtttaa agctaacaaa gataaaataa atgcattagc acacatcact
     2221 ggtggtggaa ttactgaaaa tcttccaaga gttttaccag aaaatttaac tgctgttgta
     2281 aatagaagta aaatcaaagt tttaccaata tttgaattta tgagtaagca tgtagagctt
     2341 gaagagatgt atagaacatt taatatgggt gttggaatga ttcttgttgt aaaccctgca
     2401 aatgtagatg cgattttggc ttcaactgat ggttatgtaa ttggtgagtt aaaatcaggt
     2461 gaaagaaaag tagagtttat ataaaaactc tttttttaaa gttaaaggct ggatttttta
     2521 tctagccttt ttttatatct atatatttta aaatctcaac aactttagaa gcatatcccc
     2581 actcattgtc ataccaaagc aaaagtttta tcatattttg tttaacttcc aaaaatctat
     2641 gatcaattat agttgtaaat tt
//
LOCUS       DAOQAN010000097         2662 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_54308_length_2662_cov_2.335251, whole genome shotgun sequence.
ACCESSION   DAOQAN010000097 DAOQAN010000000
VERSION     DAOQAN010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54308_length_2662_cov_2.335251"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(18..401)
                     /locus_tag="PKO10_02305"
     CDS             complement(18..401)
                     /locus_tag="PKO10_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNW66021.1"
                     /translation="MSNLIKKNGYNFSFNPTACESCAGNCCIGESGYIWINIVEIEAL
                     SKYLGLTLDSFREKYLFKVGYKYSIKEVELADNSFACCFFDLEKRKCSIYDYRPTQCR
                     TFPFWEYFKNNEKEVYKECPAIKNI"
     gene            complement(394..>785)
                     /locus_tag="PKO10_02310"
     CDS             complement(394..>785)
                     /locus_tag="PKO10_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNW66022.1"
                     /translation="YHSDVIKSEKENIKIARYNDYMPLRDFIKKSSQVLKNDGKFFFC
                     YDCKQLADIILYLKEFKLNLEALQFVHPNDSKDATLVMIYAKKNSKSLVKILNPLIVF
                     DENNEFTKTVQNIYKEAQTHSIKVFCE"
     assembly_gap    786..795
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<796..1131)
                     /locus_tag="PKO10_02315"
     CDS             complement(<796..1131)
                     /locus_tag="PKO10_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNW66023.1"
                     /translation="MVLYQPQNGYCYNSDTHFLHSFICKSLEKYKNISGEVLDIGSGS
                     GILGLLFSNKYRKTNLNQVEIQDIFQFFSIKNAKINKISSTMHKGSFLDLDFDKKFDD
                     NKEDVIKHLD"
     gene            1273..1602
                     /locus_tag="PKO10_02320"
     CDS             1273..1602
                     /locus_tag="PKO10_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011980271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNW66024.1"
                     /translation="MSNVEAPLNTPVWVNESRCKACDRCVSVCPAGVLGMRQDIHSTL
                     GSMATVIHPEACIGCNDCELSCPDFAIFVADKKEYKFSKLSDEAKQRQERIIKNNYKI
                     LDEDKKV"
     gene            1605..>2662
                     /locus_tag="PKO10_02325"
     CDS             1605..>2662
                     /locus_tag="PKO10_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014473802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate synthase subunit alpha"
                     /protein_id="HNW66025.1"
                     /translation="MSRELISTGNELAALAAIDSKCEFFGGYPITPSSEIMHVLSSKL
                     PAIGGVCMQMEDEISGICASLGAAMSGKRAMTASSGPGISLKSENLGLGYISEVPLVV
                     VNVMRGGPSTGLPTRVAQGDILQAKNPTHGDVKSITLMPGNLTECYTEVVRAFNLADR
                     FMQPVFVLLDETIGHMAGKVVLPPLSEIEKNRVFRKRFDGDKKDYLPYGAKDDEPAVL
                     NPMFEGYRYHFTGLHHGPTGHPTEDAVVCSALMSRLFKKVENHTDELELNEEYMIEDA
                     DILIIAYGSVSLGAKEAINRLRKEGIKAGMFSPKTIWPSPEKRLNELVNRFEKVLVAE
                     LNMGQYTQEIQRVSGRRD"
BASE COUNT          950 a          359 c          398 g          945 t
ORIGIN      
        1 tagaactact gttattatta aatatttttt atagctgggc actctttata tacctctttt
       61 tcattatttt taaaatattc ccaaaatgga aaagtcctac actgcgttgg tctataatca
      121 taaatagagc attttctttt ttctaaatca aaaaaacaac aagcaaaact attatctgct
      181 aattctacct cttttatgct atatttatac cctactttaa aaagatattt ttctctaaaa
      241 ctatctaaag taagtcctaa atattttgat aaagcttcta tttcaacaat atttatccaa
      301 atatatccac tttcacctat gcaacaattt ccagcacaac tttcacaagc agtaggatta
      361 aaagagaaat tgtatccatt ttttttgatt aaattactca caaaaaacct ttatactatg
      421 tgtttgagcc tctttatata tattttgtac agttttcgta aactcattgt tttcatcaaa
      481 aacaatcaaa ggatttaaaa ttttcaccaa agatttagaa ttttttttag cataaatcat
      541 taccaaagtt gcatcttttg aatcatttgg atgtacaaat tgtaaagctt caaggttcaa
      601 tttaaactct tttaaatata aaataatatc cgctaactgc ttacaatcat aacagaaaaa
      661 aaattttcca tcatttttca aaacttgtga agattttttt ataaaatctc ttaaaggcat
      721 ataatcattg tatcttgcaa ttttaatatt ctctttttca ctttttatta catcactatg
      781 ataaannnnn nnnnnatcca aatgcttaat tacatcttct ttattatcat caaacttttt
      841 atcaaaatca agatctaaaa atgagccttt gtgcattgtt gaagatattt tatttatttt
      901 tgcatttttt attgaaaaaa attgaaaaat atcttgaatc tcaacttgat ttagatttgt
      961 ttttctgtat ttattagaaa ataaaagacc taaaatacca cttccacttc caatatcgag
     1021 aacttctcca cttatatttt tatatttttc taaagattta caaataaaac tatgtaaaaa
     1081 atgtgtatca ctattgtaac aataaccatt ttgaggttga tataatacca aaaagcttcc
     1141 ttttttaaaa tctttggatt atatctaaat tatttatagt tttcttttaa taaaataagc
     1201 taaattatag taatattgtt cccaaaatta taaaatttat aaatttttat tatattttag
     1261 taaaggaaaa ttatgtcaaa tgttgaagct cctttaaata cacctgtatg ggtaaatgag
     1321 agtagatgta aagcctgtga tagatgtgta tctgtatgtc cagctggagt tcttgggatg
     1381 agacaagata ttcactcaac tttaggttct atggcaactg ttattcatcc tgaagcttgt
     1441 attggatgca atgattgtga attatcttgc cctgattttg ctatttttgt cgctgataaa
     1501 aaagagtata agttttcaaa attaagtgat gaagcaaagc aaagacaaga gagaattata
     1561 aaaaataatt ataaaatatt agatgaagat aaaaaggttt gattatgtca agagaattaa
     1621 tatcaacagg aaatgaatta gcagcattag cagctattga ttcaaaatgt gagttttttg
     1681 gtggctatcc aataacacca tctagtgaaa ttatgcacgt attatcatca aaactacctg
     1741 caattggtgg agtttgtatg caaatggaag atgagatttc tggtatttgt gcaagtcttg
     1801 gtgcagctat gagtggaaaa agagctatga cagcatcaag tggacctgga atttcactaa
     1861 aatctgagaa tttaggatta ggttatattt cagaagttcc tcttgtagtt gtaaacgtaa
     1921 tgagaggagg accttcaact ggacttccta caagagttgc tcaaggtgat attttacaag
     1981 caaaaaatcc aactcatggt gatgtaaaat ctattacttt aatgcctgga aatttaactg
     2041 agtgctatac agaagtagta cgtgctttta atctagcaga tagatttatg caacctgttt
     2101 ttgtgctttt agatgaaact attgggcata tggctggaaa agttgtactt ccacctttaa
     2161 gtgaaataga aaaaaataga gtttttagaa aaagatttga tggagataaa aaagactatc
     2221 ttccttatgg tgcaaaagat gatgaaccag ctgttttaaa tccaatgttt gaaggttata
     2281 gataccattt tacaggactt catcatggac caacaggtca tccaactgaa gatgctgttg
     2341 tttgtagtgc tttgatgagt agattattta aaaaagttga aaatcataca gatgaacttg
     2401 aactaaatga agagtatatg atagaggatg ctgatatttt aattattgct tatggttctg
     2461 tttcattagg agctaaagag gctattaatc gtcttagaaa agagggaata aaagctggaa
     2521 tgtttagtcc aaaaactatt tggccaagtc ctgaaaaaag attaaatgaa cttgtaaaca
     2581 gatttgaaaa agtattagtt gcagagctta atatgggaca atacactcaa gagatacaaa
     2641 gagttagtgg aagaagagat tt
//
LOCUS       DAOQAN010000098         2656 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_54514_length_2656_cov_2.754325, whole genome shotgun sequence.
ACCESSION   DAOQAN010000098 DAOQAN010000000
VERSION     DAOQAN010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54514_length_2656_cov_2.754325"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1131)
                     /gene="waaA"
                     /locus_tag="PKO10_02330"
     CDS             complement(<1..1131)
                     /gene="waaA"
                     /locus_tag="PKO10_02330"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HNW66026.1"
                     /translation="MSLFALFYNFILLVVYIILTPYLIYKSKSKKYKIAIPAKFFLKN
                     NPKFEKSGVWFHSCSMGEVRAIKPLIKEFEEESNISVITNTGFDEAKTICKNVRFLPF
                     EIFLPFWITRQKVLVVMEAELWYMLFLMAKKKGAKTLLINARISDKSYKSYLRFRFFY
                     KKIFENIDKVFAQSEVDKQRLQELGAKNVEVIGNIKLAQLPKKRALFEKPNITTIVAA
                     STHEGEEELILNSYKKEFGKLIIVPRHPERFLKVEELIKNYIKDKNLTFHKFSIKEDF
                     SSDIVLVDVMGILNDVYEIGDITILGGAFAKIGGHNPIEPAFFKNVIISGKNIFNQKS
                     LFECIKNYYLIENSELKEYLEKANTLLKPELTKEGSFEPIIKE"
     gene            complement(1133..1861)
                     /locus_tag="PKO10_02335"
     CDS             complement(1133..1861)
                     /locus_tag="PKO10_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013136309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HNW66027.1"
                     /translation="MNKYLQDLIALSKFDTSISQFDPKIENQKAKLAVFVETAEALKV
                     SINSVYLEIDELKSKRTKNNIHLAELKTKLDQIAKKNKEVTNEKELKALQLEEEIAKE
                     QVSFANEEIERFDKLTVAKEEKLKELQEKLKTEEEDIKEIKIAVDNEIEAINKERNLI
                     YDKRNELLDQFDKKILTFYEKIKRWAKDTAVVPVKQQACYGCFIKINDKTYAEVLKGE
                     EIVNCLHCGRILYKGEEETAVTEA"
     gene            complement(1872..2606)
                     /locus_tag="PKO10_02340"
     CDS             complement(1872..2606)
                     /locus_tag="PKO10_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004275483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HNW66028.1"
                     /translation="MLVKDIYEFLNTISPFELQEKWDNSGLLVGNMSDSFESLYLSMD
                     LDLDLVKNLKPNSLVITHHPLIFSGLKRVNYDTYSTKILKELIKKDIALISMHTNIDK
                     THLNRFVVEKILNFKIENQNEFIANVKIDMSFTDLLKHLSKKLNLKNIKFVKTKDYIK
                     TLAICTGSAMSLIDEVQADCFITGDIKYHDAMEAKARGLSLIDIRHYESENHFNILLE
                     ELLEEYLKKNKLKAIITASKNPFEFF"
BASE COUNT          848 a          428 c          254 g         1126 t
ORIGIN      
        1 ctcctttata ataggttcaa atgaaccctc ttttgttaat tctggtttca aaagagtatt
       61 tgctttttct aaatactctt ttaactcact gttttctatt aaataatagt tttttataca
      121 ctcaaaaagt gatttttgat taaaaatatt tttcccacta ataatcacat ttttaaaaaa
      181 agcgggttct attggattat gtccaccaat ctttgcaaat gctccaccta aaattgtaat
      241 atcacctatt tcataaacat catttaaaat tcccatgaca tctactaaaa ctatatcact
      301 agaaaaatcc tctttaatac taaatttatg aaaagtaaga tttttatctt ttatataatt
      361 tttaattaac tcttcaactt ttaaaaatct ctctggatgt ctaggaacaa taataagttt
      421 tccaaactct tttttataag agtttaaaat aagctcctct tcaccttcat gagtacttgc
      481 tgctactatt gttgtaatat ttggtttttc aaaaagagct cttttttttg gaagctgcgc
      541 taattttata tttccaatta cttcaacatt ttttgcacca agctcttgca atctttgctt
      601 atcaacttca ctttgtgcaa aaactttatc tatattttca aaaatttttt tataaaaaaa
      661 tctaaatctc agataagatt tatatgattt atcagaaatt cttgcattta taagtaaagt
      721 ttttgcacct ttttttttag ccattaaaaa aagcatatac caaagttctg cttccataac
      781 tactaaaact ttctgtcttg ttatccaaaa aggaagaaat atttcaaaag gtaaaaatct
      841 aacattttta caaattgttt ttgcttcatc aaatccagtg tttgtaataa cactaatatt
      901 tgattcctct tcaaactctt ttataagagg ttttatagct cttacttcac ccatagagca
      961 agagtgaaac caaacaccag atttttcaaa ttttggatta ttttttaaga aaaattttgc
     1021 aggaattgcg attttatatt ttttactttt tgacttgtag attaaatatg gagttaaaat
     1081 aatataaact acaagtaaaa taaaattata aaaaagcgca aaaaggctca aattaagcct
     1141 ctgttacagc tgtttcttct tcgcctttat aaagaattct tccacaatga agacaattta
     1201 caatctcttc accttttaaa acttcagcat aagttttatc atttatttta ataaaacaac
     1261 cataacaagc ttgttgtttt acaggaacta ctgctgtgtc ttttgcccat cttttaattt
     1321 tttcatagaa agttaatatt tttttatcga attgatctaa aagttcattt cttttatcat
     1381 aaatcagatt tctttcttta ttaattgctt caatctcatt atcaacagct attttaatct
     1441 ctttaatatc ttcttcttcc gtttttagtt tttcttggag ttcttttagt ttctcttctt
     1501 ttgcaactgt taatttatca aatctttcta tctcttcatt tgcaaaactt acttgctctt
     1561 ttgcaatctc ttcttctaat tgaagagctt ttaactcttt ttcatttgtt acttctttat
     1621 tctttttagc gatttgatct aattttgttt ttaattccgc taaatgaata ttattttttg
     1681 ttcttttaga ttttaactca tcaatctcta aataaacact atttatagaa acttttagtg
     1741 cctctgctgt ttctacaaaa acagctagtt ttgctttttg attttcaatt tttgggtcaa
     1801 attgactaat acttgtatca aactttgaca aagcaattaa atcttgtaaa tatttattca
     1861 atgttttctc cttaaaaaaa ctcaaatgga ttttttgaag ctgttattat agcttttaat
     1921 ttattttttt tcaaatattc ttctagaagc tcttccaaaa gtatattaaa atgattttca
     1981 ctctcatagt gtcttatgtc aattaaagac aatcctctag cttttgcttc cattgcatca
     2041 tggtatttta tatcaccagt tataaaacaa tctgcttgta cttcatctat caaactcata
     2101 gccgagcctg tacaaatagc taatgtttta atataatctt ttgtttttac aaattttata
     2161 ttttttaaat ttaacttttt gcttaaatgt tttaaaagat ctgtaaaact catatctatt
     2221 tttacatttg caataaactc attttgattt tcaattttga aatttaatat tttctcaact
     2281 acaaatctat ttaaatgagt tttgtcaata tttgtatgca tagaaattag tgcaatatct
     2341 ttttttatta gctcttttaa aattttagtg ctataagtat cgtaattaac tctttttaat
     2401 ccactaaaaa tcagtggatg atgagttata actaaagagt ttggctttaa attttttacc
     2461 aaatctaaat ctaaatccat acttagatat aaactttcaa aactatcact catatttcca
     2521 acaagaagtc ctgagttatc ccatttttct tgcaattcaa aagggctaat agtatttaaa
     2581 aattcatata tatcttttac taacaattta tgctcttcca actcttttta atctctcact
     2641 ctcttggctt ttgtac
//
LOCUS       DAOQAN010000099         2650 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_54699_length_2650_cov_2.483622, whole genome shotgun sequence.
ACCESSION   DAOQAN010000099 DAOQAN010000000
VERSION     DAOQAN010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54699_length_2650_cov_2.483622"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..728
                     /locus_tag="PKO10_02345"
     CDS             <1..728
                     /locus_tag="PKO10_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNW66029.1"
                     /translation="LFANTKDDCIKKGDGFIYVQNECIAYKKFNGEGDSLNIIIHGTW
                     DEGSDTLARYSPFAEDLTMMSDISTIAVALPGYSKSSSNKLLPLSNKEVKNLAATKEY
                     IEFLENLVLSLKAKYSAKKITLIAHSAGCMMSATLLGVKRDLVDNLVCVGGVYDIHKK
                     SNDKKLISAVDVVDKISKKAKIVLIYGTADEISTPQTTIDFYNLAKQKGLDVKLVEVK
                     DGVHIDLDMTTQSKDSIIELIEE"
     gene            complement(734..1657)
                     /locus_tag="PKO10_02350"
     CDS             complement(734..1657)
                     /locus_tag="PKO10_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014473414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNW66030.1"
                     /translation="MFKKIVFFTLFCTFGFASNFDYKLKPQKVSENIWCFFGKTEVPS
                     KENGGFMANSCYIKAKDSYILIDTGANYNFAKQAYEAMQKIEDLKVSTIIITHEHDDH
                     WMGNSFYKDRFNSTIYAPKSINENYNENSKPRIFEILDKNEMENTKVIKADVVVSDGK
                     VINISDKTIKIIPTKLTAHTKDDLIVYLPDEKVIFTGDIIMNQRVTSNRDGSVIGTLK
                     AIDLINSYDWNTLIAGHGTITDKKATDFTTKYFTLLKTRVLEAIEAGITADEISKVVT
                     MDDFKDIAMFDELNSRNVFDAFRELEFYDEE"
     gene            1735..>2650
                     /locus_tag="PKO10_02355"
     CDS             1735..>2650
                     /locus_tag="PKO10_02355"
                     /inference="COORDINATES: protein motif:HMM:NF024878.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HNW66031.1"
                     /translation="MDKKRQMVFNLNNFLLAFSEVLNSKKVAYIALNLGIKFGFDNKK
                     LSDVCSCALIFCLEKNSLKEFDFLDSSHLEDKTFQEIINFSTLISENFDFSKNTINQR
                     ANTLEFVKNNQKNFSKELIENFFEISKNLTFWLDLENESEIVIFIYSNLEDFTKALDF
                     ENIFKMTKVFHKLQNPKSNILDSALKVTDFFEFEHKDKQIFLIATSLQNIGKLYISKD
                     ILEKKEPLNFEEKEIIKSYPYYTKKVINSIMGFSDISSLSFKAQERLDGSGIFNLSSK
                     DLSFKDRLIICLIIYSALREQKAYREAFS"
BASE COUNT          987 a          335 c          346 g          982 t
ORIGIN      
        1 atctttttgc aaatacaaaa gatgattgta ttaaaaaagg tgatggtttt atatatgtac
       61 aaaatgagtg tattgcatat aaaaaattta atggtgaagg cgactcttta aatattatta
      121 ttcatggaac ttgggatgaa ggaagtgata ctcttgctag atattcacca tttgctgaag
      181 atttaactat gatgagtgat atttcaacta ttgctgttgc acttccaggt tactcaaaaa
      241 gttcgtcaaa taaacttctt ccactttcaa ataaagaggt taaaaactta gcagcaacaa
      301 aagagtacat tgagttttta gaaaatttag ttctatcttt aaaagctaaa tatagtgcta
      361 aaaaaataac tttaatagct catagtgctg gttgtatgat gagtgctact cttcttggag
      421 taaagcgaga tttagttgat aatttagttt gtgttggtgg agtttatgat attcataaaa
      481 aaagtaatga taaaaaacta atttctgcag ttgatgtagt tgataaaata tctaaaaaag
      541 caaaaattgt acttatttat ggaacagctg atgagatttc aacaccacaa acaactatag
      601 atttttataa tcttgcaaaa caaaaaggtt tagatgtaaa attagtagaa gtaaaagatg
      661 gtgttcatat agatttagat atgacaacac aatcgaaaga ttctattatt gaacttattg
      721 aagagtagat tttctactct tcatcataaa attctaactc tctaaaagca tcaaaaacat
      781 ttctactgtt taactcatca aacatagcaa tatctttaaa atcatccata gtgacaactt
      841 ttgaaatctc atctgctgta attccagctt caatagcttc taaaactctt gtttttaaaa
      901 gtgtgaaata ttttgttgta aaatctgtag cttttttatc agtaattgtt ccatgccctg
      961 ctattaaagt attccaatca taagaattta ttaaatcaat agcctttaaa gttcctatta
     1021 cagaaccatc tctatttgat gtaactcttt gattcataat aatatcacct gtaaaaataa
     1081 ctttttcatc aggaagatat acaattaaat catcttttgt atgagctgtt agttttgtag
     1141 ggattatctt tatagtttta tctgatatat ttataacttt tccatcactt acaacaacat
     1201 ctgcttttat aacttttgta ttttccatct catttttatc taatatctca aaaattcgag
     1261 gcttagagtt ttcgttatag ttttcattta tagattttgg agcataaatt gtgctattaa
     1321 atctatcttt ataaaaacta tttcccatcc agtgatcatc atgttcatga gttataataa
     1381 ttgtactaac ttttaaatct tcaatttttt gcatagcttc ataagcttgt tttgcaaagt
     1441 tgtagtttgc acctgtatct attaaaatat aactatcttt tgcttttata taacaagaat
     1501 ttgccataaa tccaccattc tcttttgatg gcacttcagt ttttccaaaa aaacaccaaa
     1561 tattttcact tactttttgt ggttttaact tataatcaaa atttgatgca aaaccaaaag
     1621 tacaaaaaag tgtaaaaaaa acaatctttt taaacataaa taaactctcc tttagattta
     1681 taagtgaaaa ttatattata atatcacttt taaatatgta aataagggtt ttctatggac
     1741 aaaaaacgac aaatggtatt taacttaaac aattttttac tagcttttag tgaagtttta
     1801 aatagtaaaa aagtggcata tatagcttta aatttaggta ttaaatttgg atttgataat
     1861 aaaaaattat ctgatgtatg ctcttgtgct ttgatttttt gtttagaaaa aaatagttta
     1921 aaagagtttg attttttaga tagttcacat ttagaagata aaacttttca agagattata
     1981 aatttttcaa ctttaatatc tgaaaatttt gatttttcaa aaaataccat aaatcaaaga
     2041 gccaatactc ttgaatttgt gaaaaacaat caaaaaaatt ttagtaaaga gttgatagaa
     2101 aacttttttg aaatatctaa aaatcttact ttttggcttg atttagaaaa tgagagcgaa
     2161 attgttatat ttatatactc aaatttagaa gattttacaa aagctcttga ttttgaaaat
     2221 atattcaaga tgacaaaagt atttcacaaa ctccaaaatc caaaatcaaa tatcttggat
     2281 agtgctttaa aagtaacaga tttttttgag tttgagcaca aagacaaaca aatttttcta
     2341 atagcaacat ctttacaaaa tattggaaaa ctatacattt caaaagatat tttagagaaa
     2401 aaagagcctt taaattttga agaaaaagag ataataaaat catatccata ctatacaaaa
     2461 aaagttataa actctattat gggtttttct gatatttctt ctcttagctt taaagctcaa
     2521 gaaagattag acggaagtgg aatttttaat ctatcttcaa aagatttgag ctttaaagat
     2581 aggttgatta tttgtttaat tatttatagt gcactaagag aacaaaaagc ttacagagag
     2641 gctttttctc
//
LOCUS       DAOQAN010000100         2648 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_54776_length_2648_cov_2.596606, whole genome shotgun sequence.
ACCESSION   DAOQAN010000100 DAOQAN010000000
VERSION     DAOQAN010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54776_length_2648_cov_2.596606"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..896
                     /locus_tag="PKO10_02360"
     CDS             <1..896
                     /locus_tag="PKO10_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HNW66032.1"
                     /translation="SVPAYMAARTLKNERDKLLTLFIIGFMSCGARLPIYVLFVGAFF
                     GSNNAGNILFVIYISGAILGLFAAKFLRAVVFKGKDEPFVMEMPKYRLPSLKLVYKEI
                     TNKAFMYLKKAGTFILAASILIWFMSNYPKYPNIEDEINQKIELAINDEVKKELQNEL
                     ALYNLENSYLGKLGKFSEPFFAPLGFDWKMAVALEAGLAAKEVVVSTLSILYGLGESE
                     NPDEPTTTLVEKIKTNIPFEAAISFIVFVMIYLPCLAASMVFTKEAGGWKYLVYLFIF
                     TTTTAWIMSFIAYNITKIVLA"
     gene            complement(909..>1173)
                     /locus_tag="PKO10_02365"
     CDS             complement(909..>1173)
                     /locus_tag="PKO10_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66033.1"
                     /translation="NVDCLIIEDENSIICKFEVELNNENNQTLIINWIDPNGAVSRTR
                     EMVIPAGDTSAYDFRYLDGREKGKWDFKIIYNNQEYTTKFELK"
     assembly_gap    1174..1183
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1298..2035)
                     /locus_tag="PKO10_02370"
     CDS             complement(1298..2035)
                     /locus_tag="PKO10_02370"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013136246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HNW66034.1"
                     /translation="MNFINPDLEFFEENRDCSYFEDQKSDMRYRFLNSCSIEEYQKKL
                     ERGWRRFGKIHFVPECKSCTKCVSMRIDVKNFQFSKSQKRVLNKNKDTKVFIRPPTVT
                     KEHMDLYHKYHTIMQDKKDWKYVHIDHEDYIKSYVEGNTKYATEFLYLRDDELIGVAL
                     VDILPNAISSIYCFYDHDFEEFSIGKFSILTQINIAKELNIPYIYLGYWIDNHYSMGY
                     KKEYMPFEVLKNRANLNEEPIWEKFIQ"
     gene            complement(2039..>2648)
                     /gene="trpA"
                     /locus_tag="PKO10_02375"
     CDS             complement(2039..>2648)
                     /gene="trpA"
                     /locus_tag="PKO10_02375"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014469158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HNW66035.1"
                     /translation="EGPIIEKANLLALKNGFKINDLFEVSSKIAKDIDTLWMGYMNPF
                     YHYGLENFLKKAEEFGVSGTVIPDLPYEMSQKYEPIFKKYNKSNITFVAPTTPKERIK
                     TLVENSSKFIYMVAYAGITGSGREEDLSQIIENIREYSQTPLYIGFGIDEKTCKEKSK
                     NLDGVIVGSAFVKHLLDDSLNNNEKIKKISSIAKEIKQKINE"
BASE COUNT          874 a          393 c          327 g         1044 t
ORIGIN      
        1 gtagtgttcc agcttatatg gcagctagga ctttaaaaaa tgaaagagat aaattattaa
       61 cgctatttat tattggattt atgtcttgtg gtgctagatt acctatttat gtactttttg
      121 taggtgcatt ttttggttct aataatgctg gaaatattct atttgtaatc tatattagtg
      181 gcgctatttt gggattattt gctgcaaaat ttttaagagc agtagttttt aaaggaaaag
      241 atgagccatt tgttatggag atgccaaaat atagattacc ttcactaaaa ctagtttaca
      301 aagagattac aaacaaggct tttatgtatc taaaaaaagc tggtacattt atattagcag
      361 cttcaatttt aatatggttt atgagtaact acccaaaata tccaaatatt gaagatgaaa
      421 taaatcaaaa aatagagtta gcaataaatg atgaagtaaa aaaagagtta caaaatgagt
      481 tagctttata caatcttgaa aactcatatt taggaaaact tggaaaattt agtgagcctt
      541 tttttgctcc tttggggttt gattggaaaa tggctgttgc acttgaagct ggattagctg
      601 ctaaagaggt tgttgtttcg actctatcta ttttatatgg attaggagag agtgaaaatc
      661 cagatgaacc aactactact ttagttgaaa aaataaaaac aaatattcct tttgaagctg
      721 caatctcatt tatagtattt gtaatgattt atctgccttg cttggcggct tctatggtat
      781 ttacaaaaga agctggagga tggaaatatc ttgtgtatct atttattttt actacaacaa
      841 ctgcttggat aatgtcattt atagcatata acattacaaa aatagtatta gcctaaaaaa
      901 agctactact attttaattc aaattttgtt gtgtactcct gattattgta tataatctta
      961 aaatcccact tacctttttc tcttccatct aaatatctaa aatcataggc tgaagtgtca
     1021 cctgctggta ttaccatctc tctcgttctt gaaacagcac catttgggtc aatccagttt
     1081 ataatcaatg tttgattatt ctcattattt aactcaactt caaatttaca aattattgaa
     1141 ttctcatctt ctataataag acaatcaaca tttnnnnnnn nnnaaagata gaaaaataac
     1201 taattttttc atacaaatcc ttcgttttaa atttctatta tatttaaaat ttattttaat
     1261 tataatgatt tttttaatat ttagaaaaaa actactttta ttgtatgaat ttttcccata
     1321 ttggctcttc atttaagttt gctctatttt ttaaaacctc aaaaggcata tactcttttt
     1381 tataccccat tgaatagtga ttatctatcc aatatcccaa ataaatatat ggaatattta
     1441 actcttttgc aatatttatt tgagttaaaa tagagaactt tccaatagaa aactcttcaa
     1501 aatcatgatc ataaaaacag taaattgaag atattgcatt tggtaaaata tctactaaag
     1561 caactcctat taactcatca tctctaagat ataaaaactc tgttgcgtat tttgtatttc
     1621 cttcaacata agattttata taatcttcat ggtctatatg tacatacttc caatcttttt
     1681 tatcttgcat aatagtatga tatttatggt acaaatccat atgctctttt gttacagttg
     1741 gaggtcttat aaaaactttt gtatctttgt ttttatttaa aactcttttt tgtgattttg
     1801 aaaactgaaa attctttaca tcaattctca tagatacaca ttttgtacaa ctcttacatt
     1861 caggtacaaa atgtattttt ccgaaccttc tccaacctct ttctagcttt ttttgatact
     1921 cttcaatact acaagagttt aaaaatctat atctcatatc tgatttttga tcctcaaaat
     1981 atgaacaatc tctattctct tcaaaaaact ctaaatcagg attaataaaa ttcatcttct
     2041 actcgtttat tttttgcttt atctcttttg ctatgctaga tatttttttg attttttcat
     2101 tattatttaa actatcatct aaaagatgct taacaaaagc acttccaaca ataacaccat
     2161 caagattttt tgatttttct ttacaagttt tttcatctat tccaaaacct atatatagtg
     2221 gagtttgtga atactctcta atattttcaa taatctgtga taaatcttct tctcttccac
     2281 ttccagtaat tccagcatat gcaaccatat aaataaattt agaagagttt tctacaagag
     2341 tttttattct ctcttttggt gttgttggtg ctacaaaagt gatatttgat ttattatatt
     2401 ttttaaaaat tggttcatat ttttgtgaca tttcatatgg taaatctgga attactgtac
     2461 cagaaactcc aaactcttct gcttttttta aaaagttttc taaaccatag tgataaaatg
     2521 ggttcatata tcccatccat aaagtgtcaa tatcttttgc aatttttgat gaaacttcaa
     2581 aaagatcatt aattttgaat ccatttttaa gagccaataa atttgctttt tctatgattg
     2641 gtccttca
//
LOCUS       DAOQAN010000101         2644 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_54898_length_2644_cov_2.777907, whole genome shotgun sequence.
ACCESSION   DAOQAN010000101 DAOQAN010000000
VERSION     DAOQAN010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54898_length_2644_cov_2.777907"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..824
                     /locus_tag="PKO10_02380"
     CDS             <1..824
                     /locus_tag="PKO10_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013135862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNW66036.1"
                     /translation="ISLKDMEMTQFHPTVLKGTSFARKPLLSEALRGEGAFIVDENNK
                     RFLFDYHPSGELAPRDIVSRAIFDYNKKTGLGIFLSFKNFEKKAFKQRFPNIYENIKD
                     LGYELPFENVPISPAFHYCMGGIEVALNGLVKNFKNLYAIGEVACNGVHGANRLASNS
                     LLEGLVFSRIAVNKSLEVDFKIDKQNYKKEIKEYIRNKDIDKDIKDDLRRTMWESAGI
                     VRTKKELKDALNKIESYLLLDVGRLLYLRLLTAKTILKACLKREKSLGAHFIKED"
     gene            878..2413
                     /gene="guaA"
                     /locus_tag="PKO10_02385"
     CDS             878..2413
                     /gene="guaA"
                     /locus_tag="PKO10_02385"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002933664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HNW66037.1"
                     /translation="MKHVPIVVLDFGSQYTQIIARKLRESGVYSEIVPYNESIEDIMA
                     RTPKGIILSGGPASVYASDSYHPDSTIFDLGLPILGICYGMQLIAQHFGGSVIPATSH
                     EYGKAKLDIIVENEIFKDTQNGQIVWMSHGDRVESIPSGFEKIAISENSPYAAIADTN
                     RNIYAFQFHPEVYHSECGSKLLKNFAKYICGCESTWNMGSFAKEQMTKIKNQVGNKKV
                     LCAVSGGVDSSVVATLLFEAIGNQVIPVFVDNGLLRANEREQVETIFKSRGIDLITVD
                     ASEQFLTKLAGVTDPETKRKIIGETFIEVFDKEAKKHEGVEFLAQGTLYTDVIESVSV
                     KGPSKTIKSHHNVGGLPDWMKFELVEPLREIFKDEVRELGLELGLPRNMINRHPFPGP
                     GLAIRVMGDVNKPDLDLLRKADVILLDVLHSTGYYEKTWQAFTVLLNVKSVGVMGDNR
                     TYDNTVCVRIVDATDGMTATFAHIPHEILETISRRIINEVDGINRVVYDISSKPPATI
                     EWE"
BASE COUNT         1003 a          319 c          460 g          862 t
ORIGIN      
        1 atatatcttt aaaagatatg gaaatgacac aatttcatcc aacagtttta aaaggaacat
       61 catttgctag aaaaccactt ttaagtgaag cattaagagg agaaggtgca tttattgtcg
      121 atgaaaacaa taaaagattt ctatttgatt atcatcctag tggtgagtta gctcctagag
      181 atattgttag ccgtgcaatt tttgactata acaaaaaaac aggtttgggt atatttttat
      241 cttttaaaaa ttttgagaaa aaagccttta aacaaagatt tccaaatatt tatgaaaata
      301 taaaagattt gggatacgaa cttccatttg aaaatgttcc aataagccct gcttttcact
      361 actgtatggg tggaatagaa gttgctttaa atggattagt taaaaatttt aaaaatcttt
      421 atgctattgg ggaagttgct tgtaatggtg ttcatggagc aaatagactt gcttcaaatt
      481 cacttttaga aggtcttgtt ttttcaagaa ttgcagttaa taaatcttta gaagttgatt
      541 ttaaaataga taaacaaaac tataaaaaag agataaaaga gtacataaga aataaagata
      601 ttgataaaga tataaaagat gatttaagaa gaactatgtg ggaaagtgca ggaattgtaa
      661 gaacaaaaaa agagttaaaa gatgctttaa acaaaataga gagttattta ttacttgatg
      721 ttggaagatt attgtatctt agacttttaa cagcaaaaac tatactaaaa gcttgtctaa
      781 aaagagaaaa atctttaggt gctcatttta taaaagaaga ttaaaaagag tttttagata
      841 aaatattcga ttaaaatata gaagaaaggt taaatatatg aagcatgtac caatagtagt
      901 attagatttt ggtagtcaat atacacaaat aattgcaaga aaactaagag aaagcggagt
      961 ttattctgag attgttccat acaatgaatc aattgaagat attatggcaa gaactccaaa
     1021 agggattata ctttcaggtg gtccagcttc tgtttatgct agcgattcat atcatcctga
     1081 ttctacaatc tttgatttag gacttccaat tttaggaatt tgttatggaa tgcaacttat
     1141 tgctcaacat tttggtggaa gtgtaattcc agcaacaagt catgaatatg gaaaagcaaa
     1201 actagatatt attgtagaaa atgagatttt taaagataca caaaatggtc aaatcgtttg
     1261 gatgagccat ggtgatagag ttgaatcaat tccatctgga tttgaaaaaa ttgcaattag
     1321 tgaaaattca ccttatgctg ctattgctga cacaaataga aatatctatg cttttcaatt
     1381 tcatcctgaa gtttatcact cagaatgtgg aagcaaactt cttaaaaatt ttgcaaaata
     1441 tatttgtgga tgtgaaagca cttggaatat gggttctttt gcaaaagaac aaatgacaaa
     1501 aattaaaaat caagttggaa ataaaaaagt tctttgtgct gtaagtggtg gagttgatag
     1561 ctctgttgtt gcaactcttc tttttgaagc aattggaaat caagtaattc cagtatttgt
     1621 tgataatggt cttttaagag caaacgaaag agaacaagtt gagactattt ttaaatcaag
     1681 aggtattgat ttaattactg ttgatgcaag tgagcaattt ttaacaaaat tagcaggtgt
     1741 aactgatcct gaaacaaaaa gaaaaataat tggtgaaaca tttattgaag tatttgacaa
     1801 agaggcaaaa aagcacgaag gtgttgagtt tttagctcaa ggaacacttt atacagatgt
     1861 tattgaatca gtttcagtta aaggtccttc aaaaactata aaatctcacc acaatgttgg
     1921 tggacttcct gattggatga aatttgaact tgttgaacct ttaagagaaa tctttaaaga
     1981 tgaagtaaga gagcttggac ttgagcttgg acttccaaga aatatgataa atagacaccc
     2041 attccctgga cctggacttg caattagagt tatgggagat gtaaataaac cagatttaga
     2101 ccttttaaga aaagctgatg ttattttact agatgtttta cactcaactg gatattatga
     2161 aaaaacttgg caagcattta cagtattatt aaatgtaaaa tctgttggtg ttatgggtga
     2221 taatagaact tatgataata cagtttgtgt aagaatagta gatgcaacag atggtatgac
     2281 agcaactttt gcacacattc ctcatgaaat acttgaaact atctcaagaa gaattatcaa
     2341 tgaagttgat ggaataaata gagtagttta tgatatttca tcaaaaccac cagcaactat
     2401 tgagtgggaa taaaatagag cattatcttt ttgcttgata cttcatcgtt ttataaaaat
     2461 tagcttcggt agtttacaac agtaaacttc cttgctaatt ttttaactct ttgtctgaaa
     2521 caaaaatcta aatctctatt tgaatgaaaa tataaataca cctttgccaa acttttacaa
     2581 aatctatata atactttata taaattaaac tttaatcaaa acttttattt tctaaaatgg
     2641 agaa
//
LOCUS       DAOQAN010000102         2633 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_55234_length_2633_cov_2.672227, whole genome shotgun sequence.
ACCESSION   DAOQAN010000102 DAOQAN010000000
VERSION     DAOQAN010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55234_length_2633_cov_2.672227"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /locus_tag="PKO10_02390"
     CDS             <1..605
                     /locus_tag="PKO10_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HNW66038.1"
                     /translation="PAVSIHVSQGEREFARDNKSLGMFELSDIPAAPRGVPQIEVTFD
                     IDANGVLNVSAKDKGTGKENKITISGSSGLSDDEIAKMVAEAEANKDVDAKRKALIET
                     RNQADAMLHSTRKTLEENESSISEEDKKGIIDAAASLEETLKDDNATKEQIEEKLKVL
                     TEKSHKLAEAMYKKDGEQGQGGQANQQAKKDDDVIDAEVE"
     gene            617..919
                     /locus_tag="PKO10_02395"
     CDS             617..919
                     /locus_tag="PKO10_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66039.1"
                     /translation="MQTLIYIFILFIVVLLLIYIFFKSKQNHLFSLLSGSCPSCLETR
                     KVFFDKNSNTNFTQDVIKTTVLKYHGCSGIKDVEFLCTSCGLKEIHSINSQMECNI"
     gene            916..2043
                     /locus_tag="PKO10_02400"
     CDS             916..2043
                     /locus_tag="PKO10_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HNW66040.1"
                     /translation="MKNLIFLSSLIFLITACSKKDENISIEKKYSQNSLESKYEKEIL
                     NKMEKVPFSQIKGFFDDDLNHALEVFKKDCQKSQRYEELKNVCQKAQHTNDGAMFFVS
                     NFQAYKLYDNNSNDEGMITGYYEPLLYGSLKKTQRYKYPVYKIPKDLVLSNTNSLQGY
                     KNIGKKVGKKIVPYDTRASIEKNPNNKNLEAIAYVDDKIDLFFLQVQGSGKIQLDTGE
                     ILNVGYAGQNGREYKSIGRYFIDNEIISKEDISVQAIKEALLKNPSKIDDILNINESY
                     VFFKVADQGATGALNTVLTPKRNIAVDRTYIPLGMPVFLNTQNPISKEPINKLTVAAD
                     VGGAIKGEIRADFFLGFGPEALNYAGRMKEKGKLYVLKPKY"
     gene            complement(2079..>2633)
                     /locus_tag="PKO10_02405"
     CDS             complement(2079..>2633)
                     /locus_tag="PKO10_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66041.1"
                     /translation="HRIKAIFNTTPQNVMINELVLKNKVLELKITAKDNENLDLLKQS
                     LNKIYQIVETKKLDEKQESNFEAIVVAKDELEIKDVVYGIFTQEYLQDELFDKESINE
                     QLKILLPEHSIIKYIETLNANKVEIFSFSVNTIVKEPKDLFNIFTNINSELYSITISK
                     PILMKNTNLGDEVDFIIEFNQLKN"
BASE COUNT         1035 a          310 c          408 g          880 t
ORIGIN      
        1 aaccagctgt ttctattcat gtttctcaag gtgaaagaga gtttgcaaga gataacaaat
       61 ctttaggtat gtttgaactt tcagatattc cagcagctcc aagaggtgtt cctcaaattg
      121 aagtaacatt tgatatagat gcaaatggag ttttaaatgt aagtgcaaaa gataaaggta
      181 ctggaaaaga gaacaaaatt acaatctctg gaagctctgg attaagtgat gatgagattg
      241 caaaaatggt tgcagaagct gaagcaaata aagatgtaga tgctaaaaga aaagctctaa
      301 ttgagacaag aaaccaagca gatgcaatgc ttcactctac aagaaaaact ttagaagaga
      361 atgaaagttc tattagcgaa gaagataaaa aaggaattat tgatgcagca gcatctcttg
      421 aagagacttt aaaagatgat aatgctacaa aagagcaaat tgaagagaag ttaaaagttt
      481 taactgaaaa atctcataaa ttagcagaag ctatgtataa aaaagatggt gagcaaggac
      541 aaggtggaca agcaaaccaa caagcaaaaa aagatgacga tgttatcgat gctgaggtag
      601 agtaaaactc taccttatgc aaacacttat ttatattttt attcttttta tagtagtatt
      661 actcctaata tatatctttt ttaaatcaaa acaaaatcat cttttttcac tattaagtgg
      721 tagttgtcct agttgtttag agacaagaaa agtttttttt gataaaaata gtaatacaaa
      781 ttttacacaa gatgtaatca aaacaactgt tttaaaatat catggttgtt ctggtataaa
      841 agatgtagag tttttatgta caagttgtgg tttaaaagag atacattcaa taaattctca
      901 aatggagtgt aatatatgaa gaatttaata tttttaagtt cacttatttt tttaataacg
      961 gcttgttcga aaaaagatga aaatataagt atagaaaaaa aatattcaca aaatagtctt
     1021 gaatcaaaat atgaaaaaga gattttaaat aaaatggaaa aagttccttt ttctcaaata
     1081 aaaggttttt ttgatgatga tttaaatcat gcattagaag tttttaaaaa agattgccaa
     1141 aaatcacaaa ggtatgaaga gttaaaaaat gtttgccaaa aagcacaaca cacaaatgat
     1201 ggagctatgt tttttgtctc aaacttccaa gcatataaac tttatgataa taattcaaat
     1261 gatgagggaa tgataacagg ttattatgaa ccactgttat atggaagttt aaaaaaaact
     1321 caaagatata aatatccagt ttataaaatt ccaaaagatt tagttctttc aaatacaaat
     1381 agtttacaag ggtataaaaa tattggcaaa aaagttggta aaaaaattgt tccttatgat
     1441 acaagagctt ctatagaaaa aaatccaaac aataaaaatc ttgaagcaat agcttatgta
     1501 gatgataaaa tagacctatt ctttttacaa gttcaaggaa gtggaaaaat acaacttgat
     1561 actggagaga ttttaaatgt tggatatgct ggacaaaatg gacgagaata taaaagtatt
     1621 ggaagatatt ttatagataa tgagataatt tcaaaagagg atataagtgt tcaagcaata
     1681 aaagaagcct tactaaaaaa tccttcaaaa atagatgaca ttttaaatat aaatgagagt
     1741 tatgtttttt ttaaagttgc agatcaagga gctacaggag ctttaaatac tgttttaact
     1801 ccaaaacgaa atatagcagt tgatagaaca tatattccat taggaatgcc tgttttttta
     1861 aatacacaaa atcctatttc aaaagaacct ataaataaac tcacagtcgc agcagatgtt
     1921 ggtggtgcaa ttaaaggaga aataagagct gactttttct tgggttttgg tcctgaagct
     1981 ttaaattatg ctggaagaat gaaagagaaa ggtaagttgt atgttctaaa gccaaagtac
     2041 taaaagaaca acaacttagc ttaagataaa tatatctttt aattttttag ttgattaaac
     2101 tctataataa aatcaacttc atcacctagg tttgtatttt tcatcaaaat aggttttgaa
     2161 atagtgattg agtataactc actatttata tttgtaaata tattaaataa atctttaggt
     2221 tcttttacaa tagtatttac actaaaactg aaaatctcaa ctttgtttgc attaagtgtt
     2281 tctatatatt ttatgataga gtgttctggt aagagaattt ttaactgttc atttatactc
     2341 tctttatcaa aaagttcatc ttgtaaatat tcttgagtaa atattccata tacaacatct
     2401 ttaatctcta attcatcttt tgctacaact attgcttcaa aattactctc ttgtttttca
     2461 tctaattttt ttgtctcaac aatttgatat attttattta gagactgttt tagtaaatct
     2521 agattctcat tatcttttgc agtaattttt agttctaata ctttattttt taaaaccaac
     2581 tcatttatca ttacattttg tggagtagta ttaaaaatag cttttattct atg
//
LOCUS       DAOQAN010000103         2630 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_55317_length_2630_cov_3.150291, whole genome shotgun sequence.
ACCESSION   DAOQAN010000103 DAOQAN010000000
VERSION     DAOQAN010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55317_length_2630_cov_3.150291"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1384
                     /locus_tag="PKO10_02410"
     CDS             <1..1384
                     /locus_tag="PKO10_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014469486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HNW66042.1"
                     /translation="LSGIILVLVGLFKLGNLIELVPYPVTVGFTSGIAVVIATLQIKD
                     FFGLTIENFSGTYIDKIVLLFNSFSTFNLFEFLTASATLFLLIIWRKTKSKIPAALVA
                     LGVVSIFVAFFNSFYGLNISTINSTFTYVIDGVTANGIPPIPLQFSLPWEFLKPHEIN
                     IDLLIKLLPHAIAIAILGALESLLCAVISDAMTGNKTDPNKELIGQGIANMVVPFFGG
                     IPATAAIARTVANINSGGTTKLSSIVHSIFILISILLIAPIISYLPMAALSALLLMVA
                     WNMSEVKHFVNILKTAPKDDIYVLLTCFSLTVLIDMEVAVAVGISLASILFIKRTIDL
                     YSIELVSENLETHKDMPKDILIYDINGPMFFGAAQKALKTLANINEQKNIVILNMKNV
                     SMLDMTAMVALKSIVDSFEAKNKKLIFAGLNQNVLKKLERAKFDYVTTFSNINDAIEY
                     AMHKKNFIHN"
     gene            1458..2270
                     /locus_tag="PKO10_02415"
     CDS             1458..2270
                     /locus_tag="PKO10_02415"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008336324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNW66043.1"
                     /translation="MSKVVLITGATSGMGEATAKLLSENGYKVYAGCRDKNIEKCDGN
                     INYIYLDVTKTDSIKNAVKKVIDTEGKIDVLLNNAGYGLLSTLEDGTDEEIFNQFDVN
                     VFGLIKTTREVLPYMRKENYGVIINISSFLGKMGLPLLSHYNASKYAVEGITDSLRFE
                     TLPFNIRVHSIEAGLFGTNFVKKGLVINEKTMREDSPYKKLTSHLVPIVAKAINEGPD
                     PIAIANAVKNIIEDDNSPIAIPVGAEATTFVPLRKELSNEDFEKKIIETFGL"
     gene            complement(2316..>2630)
                     /locus_tag="PKO10_02420"
     CDS             complement(2316..>2630)
                     /locus_tag="PKO10_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008244738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HNW66044.1"
                     /translation="MFTAGYDIRTREAYDKTWDEFGKIVGFEYLSGMHINDSKPELGS
                     RVDRHDSLGEGKIGWDSFKFLMNDSRMDDIPLILETIDESIWAKEIETLYSFVEDSST
                     IK"
BASE COUNT          927 a          361 c          389 g          953 t
ORIGIN      
        1 tttatctggt attattttag ttttagttgg actttttaaa ctaggcaatt taatagagct
       61 agttccttat ccagtaacag ttggatttac atctggaatt gcagttgtaa ttgcaacttt
      121 acaaataaaa gatttttttg ggcttactat agagaatttt agtggaactt atatagataa
      181 aattgttttg ctttttaact ctttttctac atttaatctt tttgagtttt taactgctag
      241 tgcaacgcta tttttactaa taatttggag gaaaacaaaa agtaaaattc cagctgcttt
      301 ggttgcttta ggagttgttt caatttttgt agcttttttt aactcttttt atggtttaaa
      361 tatttcaaca ataaactcta cttttactta tgttattgat ggagtaacag caaatggaat
      421 tcctccaata cctctacaat tttctcttcc ttgggagttt ttaaaacctc atgaaataaa
      481 tattgattta ctaataaaac ttcttcctca tgcaattgca attgcaattt tgggagcttt
      541 ggaatcactt ttatgtgcag ttataagcga tgctatgact ggaaataaaa cagacccaaa
      601 taaagagtta atagggcaag gaattgcaaa tatggttgta ccattttttg gtggaattcc
      661 tgcaactgca gcaattgcaa gaactgttgc aaatataaat tcaggaggaa caacaaaatt
      721 atcttcaata gttcactcta tttttatatt gatttcgatt ttattaattg ctccaattat
      781 ctcatatctt ccaatggctg cactatctgc tttactactt atggttgctt ggaatatgag
      841 tgaagttaaa cactttgtaa atattttaaa aactgcacca aaagatgata tttatgtact
      901 tttaacatgt ttttctttaa ctgttttaat agatatggaa gttgctgttg ctgttggaat
      961 tagccttgca tcaatactat ttatcaaacg aactattgat ttatactcta ttgagcttgt
     1021 aagtgaaaat ttggaaactc ataaagatat gccaaaagat attttaatct atgatataaa
     1081 cggacctatg ttttttggtg ctgctcaaaa agctttaaaa actttagcaa atataaatga
     1141 acaaaaaaat atagtaatcc taaatatgaa aaatgtatca atgcttgata tgacagcaat
     1201 ggttgcttta aaatccatag tagatagctt tgaagcaaaa aataaaaagc ttatatttgc
     1261 aggattaaat caaaatgttt taaaaaaact agaacgtgca aaatttgatt atgttacaac
     1321 tttttcaaat ataaatgatg cgatagagta tgctatgcat aaaaagaatt ttattcacaa
     1381 ttaagattga ttttaaacaa tatctactaa tttcttttaa gatagactcc tttttattta
     1441 aacaaaagga gttttctatg tcaaaagttg ttttaatcac aggtgctact tcaggtatgg
     1501 gagaagctac ggctaagttg ctttctgaaa atggatacaa agtttatgca ggttgtagag
     1561 ataaaaatat agaaaaatgc gatggaaata taaattatat atatcttgat gttacaaaaa
     1621 ctgactctat aaaaaatgca gtaaaaaaag taattgatac ggagggtaaa attgatgttt
     1681 tactaaataa tgctggctat ggtcttttat caactttaga agatggtaca gatgaagaga
     1741 tttttaatca atttgatgta aatgtttttg gacttataaa aactactaga gaagttttac
     1801 catatatgag aaaagaaaat tatggtgtta ttataaatat tagttcattt ttaggaaaaa
     1861 tggggcttcc tttattatct cattataatg catcaaaata tgcggttgaa ggtataacag
     1921 actctttaag atttgaaact ttgcctttta atattagagt acactctatt gaagctggac
     1981 tttttggaac aaattttgtt aaaaaaggtt tagtaataaa tgaaaaaact atgagagaag
     2041 attcacctta taaaaaatta acttctcatt tggttccaat tgttgcaaaa gcaataaatg
     2101 aaggaccaga tccaatagca attgcaaatg ctgtaaaaaa tattattgaa gatgataatt
     2161 ctcctattgc tataccagtt ggagcagaag ctacgacttt tgttcctctt agaaaagagc
     2221 taagcaatga agattttgag aaaaaaataa ttgaaacttt tggattgtga aattttcaat
     2281 tatttaaaaa aagtgagttt taaattctca cttttttatt ttatagttga actatcttct
     2341 acaaaactat ataaagtctc tatctctttt gcccaaatag actcatcaat agtctctaaa
     2401 ataagtggaa tatcatccat tcttgaatca ttcatcaaaa atttaaatga atcccaacca
     2461 atttttccct ctcctaaact atcgtgtcta tcaactctac ttccaagctc aggttttgag
     2521 tcatttatat gcattccact taaatattca aaccctacaa tctttccaaa ctcatcccaa
     2581 gttttatcat aagcttcacg tgttcttata tcataaccag ctgtaaacat
//
LOCUS       DAOQAN010000104         2623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_55527_length_2623_cov_3.176791, whole genome shotgun sequence.
ACCESSION   DAOQAN010000104 DAOQAN010000000
VERSION     DAOQAN010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55527_length_2623_cov_3.176791"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1260)
                     /locus_tag="PKO10_02425"
     CDS             complement(<1..1260)
                     /locus_tag="PKO10_02425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNW66045.1"
                     /translation="MENKNLIFRYFLYFILSFALSIFVINWFKNISLEAVLNDYKKES
                     LNKYNRYYDELIKTSDFIYFNGFIKNKKLISILQKSDENKIKDELYLEFEPEFSYYKT
                     LGIYDISFYTVDSKPILNFQDVNFDDNFNLNITQKVVASKKEFVDIKYGDKNSTIIFS
                     KPIIDEKLNLLAVVNFEFNFNQILNKLNSNSELIFEKFVSNSSDIEKDRLFILIPIFK
                     LEDSKTFYLKSNLLEKNIKIEKLNDFYNFLTIFFGLSLALIIFLLYKSKNQDIKNKLL
                     KNSYDELFSQVDRYVLKLDTDLDGKITFVTKSFCEMSGYSKDEIVGKNANILRHPDMS
                     QNFYKNLWKELRAKKIWQGEVKNIDKFGNTYWVKTSLFPKYNYKKKHIGYASIRTDIT
                     ATKQLEKTNRLLKEDLSNRLNDLKIQDE"
     gene            complement(1263..1640)
                     /locus_tag="PKO10_02430"
     CDS             complement(1263..1640)
                     /locus_tag="PKO10_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014473274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNW66046.1"
                     /translation="MSKKYSILVIDDEIEILDMLSRFLNRNPNFSVQTFSNPVSALSS
                     INNNTKYDLVLLDIVMPQMNGLDVLEKLKEINSDQKVIMMTAYSTLDKVLKSHKIGAT
                     NYVMKPFSSLDSLEKKVIEVLEA"
     gene            complement(1642..>2623)
                     /locus_tag="PKO10_02435"
     CDS             complement(1642..>2623)
                     /locus_tag="PKO10_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNW66047.1"
                     /translation="LLERHNKNMTRSLGDTQNTYLELLKELQDMQKESKETALGSTQG
                     LTLTKEVVIDTDNLYKEIASENEVVESLVSKSKDIAQVINIIQEIAFQTNILSLNAAV
                     EAATAGEAGKGFAVVAQEVRNLASRSADAAKQIKDVVTTIQKETEKIKESSDTVTSVI
                     SETKGRIESLSKLMNTFQKNSNRAVFEVESISNKIFINLAKLDHVIYKNNLYQLIFGN
                     SHNFKPVDHTQCRLGQWYNTGLGKQEFSFTHSYKGLDNPHSIVHKEANILANECAGNE
                     ISCSKELIENKIELVEKASEQVFIFLDRILEEKNEQVMKEAANKLFNEEK"
BASE COUNT          880 a          400 c          267 g         1076 t
ORIGIN      
        1 ttcatcttgg atttttaaat catttaatct atttgataaa tcttctttta gcaatctatt
       61 tgttttctca agctgttttg ttgctgttat atctgttcta atagaagcgt atccaatatg
      121 cttcttttta taattatatt taggaaacaa agaggtttta acccaatatg tatttccaaa
      181 tttatctata ttttttactt caccttgcca tatttttttt gctcttaact ctttccaaag
      241 atttttatag aagttttgtg acatatcagg gtgtcttaaa atatttgcat ttttaccaac
      301 aatctcatct ttagaatatc ccgacatttc gcaaaaactt tttgttacaa aagttatttt
      361 tccatctaaa tcagtgtcaa gcttcaatac atatctatct acttgggaaa atagttcatc
      421 ataactattt tttaaaagtt tatttttaat atcttgattt tttgatttat aaagcaaaaa
      481 aataataagt gctaaactta agccaaaaaa tatagttaaa aagttataaa aatcatttaa
      541 tttttctatt tttatatttt tttctaataa gtttgatttt aagtaaaaag tttttgaatc
      601 ttctaattta aaaattggaa ttaaaataaa aagtctatct ttttcaatat cagaagagtt
      661 tgaaacaaat ttttcaaata ttaattctga atttgaattt aacttattta aaatttgatt
      721 aaaattaaac tcaaaattta caactgctaa taaatttaat ttttcatcaa ttattggttt
      781 tgaaaatata atagtactat ttttatcacc atattttata tcaacaaact ctttttttga
      841 agcgacaact ttttgagtaa tatttagatt aaaattgtca tcaaaattta catcttgaaa
      901 gtttaatatt ggcttactat ctacagtata aaaactaata tcatatattc ctaaagtttt
      961 gtaatatgaa aactctggct caaactcaag ataaagctca tcttttatct tattctcatc
     1021 acttttttgc aaaatagata ttaatttttt gtttttgata aatccattaa agtatataaa
     1081 atcacttgtt ttaatcaact catcataata tctattgtac ttatttaaac tctctttttt
     1141 atagtcattt aaaacagctt ctaaactaat atttttaaac caatttatta caaaaataga
     1201 gagcgcaaaa gataaaataa aatataaaaa atacctaaaa attaggtttt tattttccat
     1261 atttatgctt ctaagacttc aattactttt ttttctaatg aatcaagaga actaaaaggt
     1321 ttcataacat agtttgttgc acctatttta tgagatttta aaaccttatc caaagttgaa
     1381 taagcagtca tcataataac tttttgatca ctatttatct cttttaattt ttccaaaaca
     1441 tccaaaccat tcatttgagg catcacaata tcaagtaaaa ctaaatcata ttttgtgtta
     1501 ttatttattg aactcaaagc agaaactgga ttggaaaatg tttgaacact aaaatttgga
     1561 tttctattta aaaatctgct taacatatct aaaatttcta tttcatcatc aattactaaa
     1621 attgaatact ttttactcat tttatttctc ctcattaaat agtttatttg cagcttcttt
     1681 cataacttgc tcatttttct cttcaagaat tctatctaag aatataaata cctgctcact
     1741 tgctttttca acaagttcaa ttttattttc tattaactct tttgaacaag atatctcatt
     1801 tccagcacac tcatttgcaa gaatatttgc ctctttgtga acaattgagt gtggattatc
     1861 taagccttta taagagtgag tgaaactaaa ctcttgtttt ccaagtcctg tattatacca
     1921 ctgcccaagt ctacactgtg tatgatcaac tggtttaaag ttatgtgagt taccaaaaat
     1981 tagttgatat aaattatttt tataaataac atggtcaagt ttagcaagat ttataaagat
     2041 tttatttgag atactttcaa cttcaaaaac agctctattt gaattttttt ggaatgtatt
     2101 cattaattta cttaaagact caattcttcc ttttgtctca ctaataactg atgtaacagt
     2161 atcagaactc tcttttatct tttcagtctc tttttgaatt gttgtaacaa catcttttat
     2221 ctgttttgca gcatctgcac ttctacttgc aagatttcgc acctcttgag caacaacagc
     2281 aaatcctttt ccagcttctc cagcagttgc agcttcaaca gcagcattaa gtgaaagaat
     2341 atttgtttgg aacgcaattt cttgaataat atttataact tgagcaatat ccttactttt
     2401 agaaaccaaa gattctacaa cttcattttc acttgcaatc tctttatata aattatctgt
     2461 atcaattaca acctcttttg tcaaagttaa tccttgagtt gaaccaagtg ctgtctcttt
     2521 tgactctttt tgcatatctt gaagctcttt taaaagttct aagtatgtat tttgagtatc
     2581 acctaaagat cttgtcatat ttttattgtg ccgctctagc agt
//
LOCUS       DAOQAN010000105         2598 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_56364_length_2598_cov_2.331105, whole genome shotgun sequence.
ACCESSION   DAOQAN010000105 DAOQAN010000000
VERSION     DAOQAN010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56364_length_2598_cov_2.331105"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..564
                     /locus_tag="PKO10_02440"
     CDS             <1..564
                     /locus_tag="PKO10_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510981.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HNW66048.1"
                     /translation="LANVVIMPIKMTQTSNDLEALIQKIDYDTNQVIKFLKENGLKDE
                     DITLGAVSIVDKMANEFSNQDFAMRYLASKVINIYSTEVEKIRALNSKLSELSQRGVL
                     FKTDDYDSKIEYIYTKLNEIKPSMIEEATSNARAVAQKFAQDSNSKLGKIKKASQGQF
                     EVASRDKNSDHIKNIRIVSTIEYYLAD"
     gene            complement(582..1061)
                     /locus_tag="PKO10_02445"
     CDS             complement(582..1061)
                     /locus_tag="PKO10_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014475292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66049.1"
                     /translation="MSELFNNFSTLIIFLHVISAIVWIGGMIVIRFAVHYSMQNIEDP
                     KIRLGRILENLKRFFSMVIPSIITLLITAIILILALDFKDTELYKFVIAKEIIWFIMT
                     LIFIVIYIKRDKAQKAFDGGDFLSAKNQLNPLAKYLIPINIFLGIVAVILGITLRGF"
     gene            complement(1072..>2598)
                     /locus_tag="PKO10_02450"
     CDS             complement(1072..>2598)
                     /locus_tag="PKO10_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013134249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HNW66050.1"
                     /translation="LLLNQEGIHKCHTISAGITLFNDELENTKDIFSGEVAFKLYDTY
                     GFPLDLTEDMLKDKGLKVDLDKFEELMTNQKTMAKAAWKGSGDSSNDGDFKALLEKYG
                     LNEFVGYTNTSFNSKILALLDENFKEVETLENQIGWVLLDKTPFYATSGGQNGDIGAI
                     EFEGKNAVVLETSKFHNLNLSKVDVKDTKLNKNQNIEAVVINRHEVAKHHSATHLLQA
                     ALKMVLGDSVSQAGSFNDANKLRFDFTYPKALTSEQINEVEDLVNSMIARSLKGQVEE
                     LPLEIAKKKGAIAMFGEKYGEVVRVVSFGEDVSVEFCGGTHVKNTADIGSFYIIKESG
                     VSAGIRRIEAVVGASAFKYTKEQINKLNELQAEIKSNDLIAGVKKLKSEIKELKNQIQ
                     NSQNQTQAPINEEIIGDTKVVVCVIENGDLKKIVDDMKNANEKLAILLLQEKDDKVLI
                     VAGSKNTNIKAGDWIKNIAPIVGGGGGGRPDFATAGGKDITKIEEAKIAALTYAKENL
                     "
BASE COUNT          908 a          424 c          317 g          949 t
ORIGIN      
        1 ttagcaaatg ttgtaataat gccaataaaa atgacacaaa caagtaatga tttggaagct
       61 ttgatacaaa aaattgatta cgatacaaat caagttataa aattcttgaa agaaaatggc
      121 ttaaaagatg aagatataac tttaggtgca gtttcaatag ttgataagat ggcaaatgag
      181 ttttcaaatc aagattttgc tatgagatat ttggcttcaa aggttataaa tatttatagt
      241 acagaagttg aaaaaattag agctttaaat agtaaattat cagaactttc gcaaagaggt
      301 gttcttttta aaacagatga ttatgatagt aaaatagagt atatttacac aaaattaaat
      361 gaaataaaac ctagtatgat tgaagaagca acttcaaatg caagagcagt tgcacaaaag
      421 tttgcacaag actcaaatag taaattagga aaaattaaaa aggctagtca aggtcagttt
      481 gaagttgcaa gtcgtgataa aaatagtgat cacataaaaa atattagaat agtttctact
      541 attgagtact atttagcaga ttaaaatagt ttttaagagg attaaaatcc tcttaaagta
      601 attcctaaaa taacagcaac aattcccaaa aatatattta ttggaataag atattttgcc
      661 aatggattta gttgattttt agcacttaaa aaatctccac catcaaaagc tttttgagct
      721 ttgtctcttt ttatataaat aactataaaa ataagagtca taataaacca aataatctct
      781 ttggcaatta caaatttgta tagctcggta tctttaaaat ctaaagctaa aatcaaaata
      841 atagcagtaa ttaagagagt aataatagaa ggaataacca tagaaaaaaa tctttttaga
      901 ttttctagaa ttcttcctaa tcttatcttt gggtcttcta tattttgcat agagtaatga
      961 actgcaaatc taattacaat cattccacct atccaaacta tagcagagat tacatgaaga
     1021 aaaataatta aagttgaaaa attattaaat aattcactca atttttaatc cttataaatt
     1081 ctcttttgca taagttaaag cagcaatttt tgcctcttca atttttgtta tatctttccc
     1141 accagcagta gcaaaatcag gtcttccacc gccaccacca ccaacgattg gagcaatatt
     1201 tttaatccaa tcaccagctt taatatttgt atttttactt ccagcaacga ttaaaacttt
     1261 gtcatctttt tcttgtaaaa gtaaaattgc taatttttcg tttgcatttt tcatatcatc
     1321 aacaattttc tttaaatcac cattttcaat aacacaaact acaacttttg tatcaccaat
     1381 aatttcttca tttattggag cttgagtttg attttgtgaa ttttgtattt gatttttaag
     1441 ctcttttatc tcagatttga gttttttaac accagcaatt aaatcatttg atttaatctc
     1501 agcttgaagt tcatttagtt tgtttatctg ctcttttgta tatttaaatg cacttgctcc
     1561 tacaacagcc tctattcttc ttattcctgc acttactcca gactctttta tgatataaaa
     1621 acttccaata tctgctgtat tttttacgtg agttcctcca caaaattcaa cagaaacatc
     1681 ttcaccaaag ctaactactc ttacaacttc accatatttt tcaccaaaca tagcaattgc
     1741 accttttttc tttgcaatct ctaaaggtaa ctcttcaact tgacctttta atgatcgtgc
     1801 aatcatagag ttaactaaat cttcaacttc atttatctgc tcacttgtta aggcttttgg
     1861 gtatgtaaag tcaaatctta gtttatttgc atcgttaaat gatccagctt gagaaacact
     1921 atctcctaaa accattttta aagcagcttg aagtaagtga gtagcactat gatgttttgc
     1981 aacctcgtgt ctattaataa caacagcttc aatgttttga tttttattta attttgtatc
     2041 ttttacatct acctttgata agttaagatt atgaaatttt gaagtctcaa gtactactgc
     2101 attttttcct tcaaattcga ttgctccaat atctccattt tgtccaccac tagttgcata
     2161 aaaaggagtt ttatctaaaa gtacccatcc aatttgattt tccaaagttt ctacttcttt
     2221 aaaattttca tcaagtaaag ctaaaatttt tgaattaaaa ctagtatttg tatatccaac
     2281 aaactcgttt aatccatatt tttcaagtaa agctttaaaa tcaccatcat ttgaactatc
     2341 tccactacct ttccaagcag cttttgccat agttttttga tttgtcataa gctcttcaaa
     2401 tttatctaaa tctactttta gacctttgtc ttttagcata tcttctgtta aatcaagagg
     2461 gaatccataa gtgtcatata gtttaaatgc aacttctcca ctaaaaatat ctttagtatt
     2521 ttctagttca tcattaaata gtgtaattcc tgcagaaatt gtatgacact tatggattcc
     2581 ctcttgattt aacagaag
//
LOCUS       DAOQAN010000106         2586 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_56767_length_2586_cov_3.445279, whole genome shotgun sequence.
ACCESSION   DAOQAN010000106 DAOQAN010000000
VERSION     DAOQAN010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56767_length_2586_cov_3.445279"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..160
                     /locus_tag="PKO10_02455"
     CDS             <1..160
                     /locus_tag="PKO10_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000954073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HNW66051.1"
                     /translation="LSGLIASLLAQGYTSLEAAINASLAHTIAAKKYKNNNYSMSPQD
                     IIQGIKIL"
     gene            157..468
                     /gene="cutA"
                     /locus_tag="PKO10_02460"
     CDS             157..468
                     /gene="cutA"
                     /locus_tag="PKO10_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HNW66052.1"
                     /translation="MKAVIVQTTTSNEEEAKKIAKILIQDKLAACVQLKDIESLYNWD
                     GKLCCERETLLSIKTKKELFSKVKSKILELHSYDTPEIIELDISNISEDYLKFIKENT
                     I"
     gene            465..1244
                     /locus_tag="PKO10_02465"
     CDS             465..1244
                     /locus_tag="PKO10_02465"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HNW66053.1"
                     /translation="MSDILKIGKYELNSRLIVGSGKYKDFQTTKEATLASGSELITVA
                     IRRVNITNPNEENLLDYFKDTNIKFLPNSAGCKNAQETVTTFRLMREATGIDLIKLEI
                     IGDFSKTLYPDVIETIKACEILKKDGFTVMAYTSDDLIVAKRLEDAGADAIMPLAAPI
                     GSGLGVQNRYNIAFIKDSVKVPVIVDAGVGCASDAAICMELGADGVLTNTAIAGAKDP
                     ISMAIAMKHAVIAGRLGYKAGRIEKKPYASASSPLDGLIQF"
     gene            1354..1431
                     /locus_tag="PKO10_02470"
     tRNA            1354..1431
                     /locus_tag="PKO10_02470"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1389..1391,aa:Pro,seq:tgg)
     gene            1451..1527
                     /locus_tag="PKO10_02475"
     tRNA            1451..1527
                     /locus_tag="PKO10_02475"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1485..1487,aa:His,seq:gtg)
     gene            1543..1619
                     /locus_tag="PKO10_02480"
     tRNA            1543..1619
                     /locus_tag="PKO10_02480"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1577..1579,aa:Arg,seq:tct)
     gene            1661..1745
                     /locus_tag="PKO10_02485"
     tRNA            1661..1745
                     /locus_tag="PKO10_02485"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1695..1697,aa:Leu,seq:tag)
     gene            1774..1850
                     /locus_tag="PKO10_02490"
     tRNA            1774..1850
                     /locus_tag="PKO10_02490"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1808..1810,aa:Gly,seq:tcc)
     gene            complement(1897..2022)
                     /locus_tag="PKO10_02495"
     CDS             complement(1897..2022)
                     /locus_tag="PKO10_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66054.1"
                     /translation="MKIFKYIILILLINLLFTGCFLSTNINSGGSVGMSVGGSIF"
     gene            complement(2145..>2586)
                     /locus_tag="PKO10_02500"
     CDS             complement(2145..>2586)
                     /locus_tag="PKO10_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014473005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HNW66055.1"
                     /translation="VRKNEKKDESKKIGENKMSNGNYTVVVDGQRFNVSVFEGDVQNI
                     QVAQPVQQVVQQAPVQAPIQAAPVAASKPVYNGTEAIAPVNGNVWKILVKEGDRVEKD
                     QQIMILEAMKMEIDVVAPVSGTISKILTEPSKAVEEGQTLAVIS"
BASE COUNT          866 a          377 c          469 g          874 t
ORIGIN      
        1 tttatcaggt ttaatagcat ctttattagc ccaaggttat acatcattag aagctgcaat
       61 aaatgctagt ttagcgcata caatagcggc aaaaaaatat aaaaataata actattctat
      121 gagtccacaa gatataattc aaggtataaa aattttatga aagcagttat agttcaaact
      181 acaacttcta atgaagaaga agcaaaaaaa atagctaaaa ttttgattca agataagctt
      241 gctgcttgtg tgcagttaaa agatatagaa tctctttata attgggatgg taaattatgt
      301 tgtgaaagag aaacactttt aagtataaaa acaaaaaaag agcttttttc taaagttaaa
      361 agcaaaatat tagaacttca tagttatgat actccagaaa ttatcgaatt agatatttca
      421 aatattagtg aagattattt aaaatttata aaggaaaata caatatgagt gatattttaa
      481 aaataggaaa atatgagtta aatagtagat taatagttgg aagtggtaaa tataaagatt
      541 tccaaactac taaggaagca acacttgcaa gtggaagtga gttaataact gtagcaataa
      601 gaagagtaaa tattacaaat ccaaatgaag aaaatctctt agattatttt aaagatacaa
      661 atattaagtt tttaccaaat agtgctggat gtaaaaatgc ccaagagacg gtgacaactt
      721 ttagacttat gagagaagct acaggtattg atttaattaa attagagatt attggagatt
      781 ttagtaaaac tttgtatcca gacgttattg aaactattaa agcttgtgaa atcttaaaaa
      841 aagatggttt tacagttatg gcatatacaa gtgatgattt aattgttgca aaaagacttg
      901 aagatgctgg tgctgatgct attatgcctt tggctgctcc aattggaagt ggtttaggtg
      961 ttcaaaatag atataatatt gcatttataa aagatagtgt aaaagttcct gttatagttg
     1021 atgctggagt tggatgtgct agtgatgctg ctatttgtat ggagcttggt gctgatggag
     1081 ttttgacaaa tactgcaata gctggagcaa aagatccaat ctcaatggca atagctatga
     1141 aacatgcagt tatagctgga agattagggt ataaagctgg aagaatagag aaaaaacctt
     1201 atgcaagtgc atcttcgcct ttggatggtt taatacaatt ttagacaatt tcaagaaatt
     1261 ttgtattttt tacaattttt cttgaaaagc tcttgacaaa aggaaaaata tataatataa
     1321 ttccgtccac tttttgagtt aaaaggtgtt tatcggggcg tagcgcagtc tggttagcgc
     1381 acctggtttg ggaccagggg gccggaggtt cgaatcctct cgccccgacc attaggactt
     1441 aaaatttatg gtaggtatag ctcagtcggt tagagcatcg ggttgtggtt ccgagggtcg
     1501 tgggttcgag ccccattatc taccccattt ttatattttt atgcttccat agctcagctg
     1561 gatagagcaa cgcccttcta aggcgtaggc cgtacgttcg aatcgtactg ggagtaccat
     1621 tttggtttag aataccaact aaatttaaaa tttattttat gcggatgtgg tgaaattggt
     1681 atacacgcca gacttaggat ctggtgcctc acggtgtaag agttcgagtc tcttcatccg
     1741 caccacttat acaaacaaaa aattaatagt tttgcgggag tagctcagtt ggctagagct
     1801 tctgccttcc aagcagactg tcgcgagttc gagtctcgtc tcccgctcca ttaagccttg
     1861 tctaagagat atcttttagg caaggctttt ttatttttaa aaaatacttc caccaacact
     1921 cataccaact gaacctccac tatttatatt tgtactaaga aaacaaccag taaaaagtaa
     1981 gtttataaga agtattaaaa ttatatattt gaaaattttc attagattct cttttgtatt
     2041 ttttgatatt ttataataag gattattaaa agaagagttg aagtatataa aaataaattt
     2101 atagtttaaa gatgtgctta tcgtaaaaga taagcacttt ttatttaaga aataactgct
     2161 agagtttgac cctcttcaac tgcttttgaa ggttctgtta aaattttact tattgttcca
     2221 gaaactggag caacaacatc tatttccatt ttcatagctt caagtatcat tatttgttga
     2281 tctttttcaa ctctatcacc ctctttaaca aggattttcc atacatttcc atttacagga
     2341 gctattgctt ctgttccatt gtaaacaggt tttgaagctg ctactggagc tgcttgaata
     2401 ggtgcttgaa caggtgcttg ttgaactact tgttgaacag gttgtgcaac ttgaatattt
     2461 tgaacatctc cttcaaaaac tgatacatta aatctttgac catctactac aactgtataa
     2521 tttccgtttg acattttatt ttctcctatt tttttagatt catctttttt ttcatttttt
     2581 cttaca
//
LOCUS       DAOQAN010000107         2586 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_56781_length_2586_cov_1.784275, whole genome shotgun sequence.
ACCESSION   DAOQAN010000107 DAOQAN010000000
VERSION     DAOQAN010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56781_length_2586_cov_1.784275"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="PKO10_02505"
     CDS             <1..607
                     /locus_tag="PKO10_02505"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNW66056.1"
                     /translation="VYENNLKYKIDFKNRNIGLFLDMKKGREYIKSICKDKNVLNLFS
                     YTCAFSVVSICGEASSVVNIDMSKASLSTGRINHHLNQLDTKNVKFLPYNILKSFGKI
                     KKMSPYDIVIIDPPSFQKGSFVATSDYIKIIKKLDFILPIGGVVLACLNDPFLNSNFL
                     IDIFKKEAPNFEFLEKLENVDEFLVENEEKALKNLIFLKKN"
     gene            641..2263
                     /locus_tag="PKO10_02510"
     CDS             641..2263
                     /locus_tag="PKO10_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012013108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNW66057.1"
                     /translation="MEHFLFTLFMAIFLATVLNIILKKLGISQIIGYILTGIIISYGF
                     HFKGANIASLDAIAEFGIVFLMFTIGLEISFDKIRKMKEILLLNGFLQVHISALLIFA
                     VTHFIFDLSIEVSIIIAFAFSLSSTAIVLPYLKSSKDIYTPYGERTTAILIYQDLAVI
                     PILLLISFLTNDNLSIGEVIWNTFVSAAVITIFMFFFGKIIDWLLQFSSSTRLEELFI
                     GAVFSIVIGASLLAEYLGFTYSLGAFLAGMIIADTKFRIKVESDISNFKDLLLGTFFF
                     TVGTKIDILYFLNNIHIVIGLFLLIMIIKALVVFLIIRRKSDKNTSVKSAIALCQVGE
                     FSFAILTLAASQNVVPVETANFLVLISVMSMILTPFLLNNIYKISDLISADIYQSENL
                     EPIEDTNHIIICGFSILGRVVARDLADRNMPFVIVSNDIRQIQVATKMGYKAYFGHLD
                     KKSVLEALKVEDSSSIIITINETHEKILICDAILKYCPDANIILKYESLEERHVLNDL
                     KIKKFVHAHAEIGRLLVEEATHCCDLRLHKYD"
     gene            2341..>2586
                     /locus_tag="PKO10_02515"
     CDS             2341..>2586
                     /locus_tag="PKO10_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014474110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HNW66058.1"
                     /translation="MKFSKIIILFLLLLSSLFANQHKKLAYIVSDTTIPFWQIMSKGI
                     KEKAVEFGYEIDIYNSNNLTKNELENMALALDKKIDGL"
BASE COUNT          951 a          277 c          360 g          998 t
ORIGIN      
        1 tgtgtacgaa aataacttaa aatataaaat agattttaaa aatcgaaata tcggactttt
       61 tttagatatg aaaaaaggta gagagtatat aaaatctatt tgcaaagata aaaatgtttt
      121 aaatcttttc tcatatactt gtgcttttag cgtagtttct atttgtggcg aagcttcaag
      181 tgtagtgaat attgatatgt caaaagctag tttatctact ggaagaataa atcatcatct
      241 aaatcaatta gatacaaaaa atgtaaaatt tttaccatat aatattttaa aatcatttgg
      301 taaaatcaaa aaaatgtcac cctatgatat tgttataatt gatcctccct catttcaaaa
      361 aggctcattt gttgccacta gtgattatat taagattatc aaaaaacttg attttatatt
      421 accaattggt ggagtagttt tagcttgttt aaatgaccca tttttaaata gtaatttttt
      481 aatagatata tttaagaaag aagcaccaaa tttcgagttt ttagaaaaat tggaaaatgt
      541 agatgaattt ttagtagaaa atgaagaaaa agctcttaaa aatctaattt ttttaaaaaa
      601 aaactgatat aatccaacat ttttaaagaa agaaatcaca atggaacact tcttatttac
      661 actatttatg gcaatttttc tagcaactgt cctaaatata attttaaaaa aattaggtat
      721 ttcacaaatc ataggttata ttttaactgg aattatcata tcttatggat ttcattttaa
      781 aggtgctaat atagcatctt tagatgcaat agcagaattt ggaatagttt ttctaatgtt
      841 tacaataggt cttgaaataa gttttgacaa aattagaaag atgaaagaga ttcttttatt
      901 aaatggtttt ttacaagttc atattagtgc attgcttatt tttgctgtta ctcactttat
      961 ttttgatttg tcaatagaag tttcaataat tattgctttt gcattttctc tatcttcaac
     1021 agctattgtt ttaccttatt taaaatcttc aaaagatatt tatactcctt atggtgaaag
     1081 aacaacagct attttaatct atcaagattt agcagttatt ccaatactac ttttaatttc
     1141 atttttaaca aatgacaatt tatctatagg agaagtaatt tggaatactt ttgtctcagc
     1201 tgctgttatt acaatattta tgttcttttt tggaaaaata atagattggt tacttcaatt
     1261 ttcatcaagt acaagacttg aggagctctt tatcggggct gttttttcaa tagttattgg
     1321 tgcatcttta ttggctgaat atttaggatt tacatactct ttgggtgctt ttttagctgg
     1381 aatgattatt gctgatacaa aatttagaat aaaagttgaa tcagatattt caaatttcaa
     1441 agatttactt ttaggaacat tcttctttac agttggaaca aaaatagata ttttatactt
     1501 tttaaataat attcatattg taataggtct atttttatta ataatgataa taaaagcatt
     1561 ggttgtattt ttaattatta ggagaaaatc agataaaaat acatctgtaa aatcagcaat
     1621 tgcactttgt caagttggtg agttctcttt tgctatttta actcttgcag caagtcaaaa
     1681 tgtagttcct gttgaaactg caaatttttt ggttttgatt tcggtaatgt caatgatttt
     1741 gacaccattt ttattaaaca atatatataa aatatcagat ctaatttcag ctgatattta
     1801 tcaatcagaa aatttagaac ctattgaaga tactaatcat attattattt gtgggttttc
     1861 aattttagga agagttgttg ctagagattt agcagataga aatatgcctt ttgtaattgt
     1921 ttcaaatgat attagacaaa tccaagttgc aacaaaaatg ggttataaag catattttgg
     1981 tcacttagat aaaaaatcag ttttagaagc tttaaaagtg gaagatagtt caagtattat
     2041 aataactatt aatgaaacac atgaaaaaat acttatttgt gatgccatat taaaatattg
     2101 tccagatgca aatattattt taaaatatga atctttagaa gagagacatg ttttgaatga
     2161 tttaaaaatt aaaaaatttg ttcacgcaca tgctgaaatt gggagattat tggtagaaga
     2221 ggcaactcat tgttgtgatt taagacttca taaatatgat taattttatt taaaaataaa
     2281 cttaaaattt atgaaattta gataatatat tttttaatat aatcattatt aggggaatga
     2341 atgaaatttt caaaaattat aattttgttt ttattgcttt tgtcttcttt atttgcaaat
     2401 cagcataaaa aattagctta cattgtttca gatacaacta ttcctttttg gcaaattatg
     2461 tcaaaaggaa taaaagaaaa agcagttgaa tttggatatg aaatagatat ttataactca
     2521 aataatctaa cgaaaaatga acttgaaaac atggctcttg ctttagataa aaaaatagat
     2581 ggttta
//
LOCUS       DAOQAN010000108         2575 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_57130_length_2575_cov_3.023810, whole genome shotgun sequence.
ACCESSION   DAOQAN010000108 DAOQAN010000000
VERSION     DAOQAN010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57130_length_2575_cov_3.023810"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="PKO10_02520"
     CDS             complement(<1..208)
                     /locus_tag="PKO10_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66059.1"
                     /translation="MIEEIAEKKMVERKTEKIVKEEVVEKSTSASNEKKPDLKSLFAN
                     VKETSNKVVKEEVNNVEKSIDPKRF"
     gene            complement(358..747)
                     /locus_tag="PKO10_02525"
     CDS             complement(358..747)
                     /locus_tag="PKO10_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014474776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNW66060.1"
                     /translation="MFDFNQKPDLNITPLVDIMLVLLAILMVTAPVIEFEEPINLPTG
                     SKSQQSQDVAKIDIIITKDRIVTLNKNKVEISNFADNFLLFSKGKDQNTPIHIRADKS
                     LKYDDIVYVLKSVKEAGFFKVALVTDG"
     gene            complement(751..1311)
                     /locus_tag="PKO10_02530"
     CDS             complement(751..1311)
                     /locus_tag="PKO10_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNW66061.1"
                     /translation="MISTLLNYLANSSAITYIVLALLSVYTIVVFWIFFYRNNALNRL
                     IANEKRSLEILTTSQSKFSPLSLLTQCSNGTASKEILHACEINIIKDASNGVSWLAII
                     SSTSPFIGLFGTVVGILESFAKFANQSKVAFSIIAPAISEALVATAAGIFVAIFAYTF
                     HQIISRKIYELNIYLKAQSEIIVAKG"
     gene            complement(1318..1695)
                     /gene="atpC"
                     /locus_tag="PKO10_02535"
     CDS             complement(1318..1695)
                     /gene="atpC"
                     /locus_tag="PKO10_02535"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014474778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HNW66062.1"
                     /translation="MDTIKLSIVTPTGSIFNGDVKTVTLPGKEGEFGVLPGHSSLVST
                     LSVGVIVIEKIDSTEAVAINWGHVKVDEKSVDVLVDGAIALTGGKDSEISKNLEAAKE
                     LVNSVKDSKVSMAAVEAKINSFA"
     gene            complement(1714..>2575)
                     /gene="atpD"
                     /locus_tag="PKO10_02540"
     CDS             complement(1714..>2575)
                     /gene="atpD"
                     /locus_tag="PKO10_02540"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002343567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HNW66063.1"
                     /translation="FAGVGERTREGNDLYHEMKDSNVLDKVALCYGQMSEPPGARNRI
                     ALTGLTMAEYFRDEKGLDVLMFVDNIFRFAQSGSEMSALLGRIPSAVGYQPTLASEMG
                     KLQERITSTSKGSITSVQAVYVPADDLTDPAPASVFAHLDATTVLNRKIAEKGIYPAV
                     DPLDSTSRILSADIIGQEHYNTARGVQSVLQKYKDLQDIIAILGMDELSEADKLVVAR
                     ARKIERFLSQPFFVAEVFTGSPGKYVELKDTIAGFQGILDGKYDHIPEMAFYMVGGID
                     EVLAKAEKMK"
BASE COUNT          846 a          446 c          344 g          939 t
ORIGIN      
        1 taaatctttt aggatcaata gatttttcaa cattattaac ctcttctttt acaactttat
       61 tagatgtctc tttaacattt gcaaataaag attttaagtc tggttttttt tcatttgaag
      121 ctgaagtaga tttttcaaca acttcttctt ttacaatctt ttctgttttt ctttcaacca
      181 tttttttctc tgctatctct tctatcatat caagttctat ggtagttgag cttggagtta
      241 tgttaatact ctccttgatt ggagaaaata tataatacat aactaaaaaa catattgaaa
      301 aatatataaa aaatgcaata ataccggaaa taataaatga agaattattt tgcattttta
      361 tccatctgtt actaaagcaa ctttgaagaa accagcttct ttaactgatt ttagaacata
      421 tacaatatca tcatatttca agcttttgtc tgctcttata tgtattggag tattttgatc
      481 tttacctttt gaaaagagca aaaagttatc tgcaaaattt gatatttcaa ccttattttt
      541 atttaaagta acaattctat cttttgtaat aataatatca attttagcaa catcttgaga
      601 ttgttgagat ttacttcctg ttggaagatt tataggttct tcaaactcaa taacaggtgc
      661 tgtaaccatt aaaattgcaa gaagtacaag cataatatca accaacggtg taatatttaa
      721 atctggtttt tgattaaaat caaacaattt ttacccttta gctactatta tttcagattg
      781 agcttttaaa tagatattta attcatatat ttttcttgaa ataatttggt ggaatgtata
      841 agcaaatatt gcaacaaaaa tacctgcagc agttgctact aatgcttcac taatagctgg
      901 agctataatc gaaaacgcaa cttttgattg atttgcaaat tttgcaaatg actctaatat
      961 tccaacaact gttccaaaaa gacctataaa tggagaagtt gatgagataa tagctaacca
     1021 agatacacca ttacttgcat cttttataat atttatctca caagcatgta aaatttcttt
     1081 tgaagcagtt ccatttgaac actgtgttaa aagtgaaagt ggagaaaatt ttgattggct
     1141 tgttgttaaa atttctaaag atcttttttc atttgctatt aatcggttta atgcattatt
     1201 tctataaaag aaaatccaaa aaacaactat cgtataaact gacaatagcg ctaaaactat
     1261 ataagttata gcgctactat ttgccaaata atttagtaat gtagaaatca tatattttta
     1321 tgcaaatgaa ttaatttttg cttcaactgc agccatagat actttagaat ctttaactga
     1381 gtttaccaac tcttttgcag cttcaagatt ttttgaaatt tctgaatctt ttccgcctgt
     1441 taatgcaata gctccatcaa ctaatacatc aactgatttt tcatctacct taacatgtcc
     1501 ccagttaata gcaacagctt cagtagaatc tattttttca ataacaatta caccaacact
     1561 aagagttgat actaatgatg agtgtccagg taaaacacca aactctccct cttttccagg
     1621 gagagttaca gttttaacat caccattaaa tatgctacca gttggagtaa ctattgataa
     1681 tttaattgta tccatactat aaccctttat ggtttatttc attttctcag cttttgctaa
     1741 aacctcatca attccaccaa ccatatagaa tgccatttca gggatatggt catatttacc
     1801 atctaaaatt ccttggaatc ctgctattgt atctttcaac tcaacatatt ttccaggact
     1861 tcctgtaaat acttcagcaa cgaagaatgg ttgtgataag aatctttcaa tttttcttgc
     1921 acgtgcaact acaagtttat cagcttctga taattcgtcc ataccaagaa ttgcaataat
     1981 atcttgtaaa tctttatatt tttgaagaac cgattgaaca cctcttgcag tgttatagtg
     2041 ttcttgtcca ataatatctg cacttaagat tcttgaagta gaatctagtg gatcaaccgc
     2101 tggataaata cctttttctg caatttttct atttaaaact gtagttgcat ctaagtgagc
     2161 aaaaacagaa gctggtgctg gatccgttaa gtcatccgct ggaacgtaaa cagcttgaac
     2221 tgaagtaata gaacctttag aagttgaagt aattctctct tgtaatttac ccatttcgct
     2281 tgcaagtgtt ggttggtatc caacagctga aggaattctt cctaaaagtg ctgacatttc
     2341 agaacctgat tgtgcaaatc taaagatatt atcaacgaac ataagaacat ctagtccttt
     2401 ttcatctcta aaatattcag ccattgtaag acctgtaagt gcaattctat ttctagcacc
     2461 tggtggttca ctcatttgac catagcacag tgcaacttta tcaagaacgt ttgaatcttt
     2521 catctcatga taaaggtcat ttccttctct tgttctttca ccaactccag caaat
//
LOCUS       DAOQAN010000109         2574 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_57172_length_2574_cov_2.663358, whole genome shotgun sequence.
ACCESSION   DAOQAN010000109 DAOQAN010000000
VERSION     DAOQAN010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57172_length_2574_cov_2.663358"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1330)
                     /gene="glyS"
                     /locus_tag="PKO10_02545"
     CDS             complement(<1..1330)
                     /gene="glyS"
                     /locus_tag="PKO10_02545"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HNW66064.1"
                     /translation="MLKPLLIEIGVEELPAIPFLKELSNIEKKWSDILEKNSLLCNFE
                     LFYTPRRLVLWHREFQVKQEDSEVENIGAPRSIAFKDGVATPAAASFASKCGISVDEI
                     LFKDFGKGEVLYHKQKISGAFSKELLNNMVNEFVASLNFGKSMRWGSRSDSFIRPIRF
                     FSIMMDNEIVEGELFGVKSSNLSYGHRMDSYEPFIFNDVGDYFCKLDKYGVVLYQDER
                     REKILKQIKDIEIKHNVKIELDLELLDEVVAITEYPTALLGKFDEEFLELPAEVIVTS
                     MKANQRYFAVYKNEKLTNNFVVISNALTNDFGYIISGNEKVLRPRLADAMFFYKNDIK
                     NGLSNEGLKKLVFVEGLGTVYDKCEREAKIALYLAEILKIDKKDIELLQKAVMLSKAD
                     LMSEMVYEFTELQGLMGYYYSKIAKNDEKISLALKEQYLPSGENSELPSSI"
     gene            complement(1323..1490)
                     /locus_tag="PKO10_02550"
     CDS             complement(1323..1490)
                     /locus_tag="PKO10_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66065.1"
                     /translation="MKDILDFVVLALFVFMVAMFIINFNRQQIKKHTDKLEKLEEIEK
                     NKNQDKEKENA"
     gene            complement(1487..1867)
                     /locus_tag="PKO10_02555"
     CDS             complement(1487..1867)
                     /locus_tag="PKO10_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66066.1"
                     /translation="MKIIGDKLIPFEAFSKVLSIEDIKNTKPNSMIFFDFNEELLKYS
                     FFQHLNFLVYVKSIKEAIYASNFNAKYIICENELAKKLQKIADNYMWDSKILTIIKSS
                     DDFEKVALEEIDGAIYSDLLEIKV"
     gene            complement(1869..2090)
                     /locus_tag="PKO10_02560"
     CDS             complement(1869..2090)
                     /locus_tag="PKO10_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014474272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP0621 family protein"
                     /protein_id="HNW66067.1"
                     /translation="MLIKVIAVVLAGFVVYLLFFKNKRVDGVKKNDKLISDEMVECPT
                     CSTFVSQKEAIVSSGKFFCSKDCLNNKKA"
     gene            complement(2084..>2574)
                     /gene="rsmG"
                     /locus_tag="PKO10_02565"
     CDS             complement(2084..>2574)
                     /gene="rsmG"
                     /locus_tag="PKO10_02565"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013135474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HNW66068.1"
                     /translation="VHNLSGRLSDDDIYENILDSLYPLNFIDEFNSFADIGTGAGYPG
                     LILAIARKDVKSYLIEPRVKRVAFLNFVKASLGLNNLTILQKRAENVEGLEVDLITSR
                     AVTNTNLLLNITSKIKKDYTSYLFYKGSMLEDEIETSKISNYKVVSRCERNYLYIKGK
                     KC"
BASE COUNT          897 a          457 c          218 g         1002 t
ORIGIN      
        1 aaattgaact aggaagttca ctattctcac cacttggaag atactgctct tttagtgcca
       61 aactaatctt ttcatcattt tttgcaattt tagaatagta gtaacccata agcccttgaa
      121 gttctgtaaa ctcataaacc atttcgctca ttaaatcagc ttttgaaagc attacagctt
      181 tttgcaatag ttcaatatct tttttatcta tttttaaaat ctctgccaaa tataaagcaa
      241 tttttgcttc tctttcacat ttatcataaa ctgttcctaa accttcaaca aatacaagtt
      301 ttttaagtcc ctcatttgat aaaccatttt taatatcatt tttataaaaa aacattgcat
      361 ctgctaatct tggtcttaaa accttctcat ttccagagat tatatatcca aaatcatttg
      421 ttaatgcatt tgaaataact acaaaattgt ttgtaagttt ctcattttta taaactgcaa
      481 aatatctctg atttgctttc attgatgtaa caattacttc tgctggaagt tccaaaaact
      541 cctcatcaaa ttttcctaaa agtgctgttg gatactcagt aattgcaaca acttcatcta
      601 ataactctaa gtccaattct attttaacat tatgtttgat ttcaatatct tttatttgtt
      661 ttaagatttt ttctcttctt tcatcttgat ataaaactac tccatattta tctaacttac
      721 aaaaataatc acctacatca ttaaaaataa atggttcata actatccatt ctatgaccat
      781 aagataaatt agaagatttt actccaaaaa gctcaccttc tacaatttca ttatccatca
      841 ttatcgaaaa aaatctaata ggtcttatga aactatcact tctacttccc catctcatag
      901 attttccaaa atttaaactt gctacaaact catttaccat attatttaaa agctcttttg
      961 aaaaagctcc agagattttt tgtttatgat ataaaacttc accttttcca aaatctttaa
     1021 ataaaatttc atctacacta attccacatt ttgatgcaaa actagcagct gcaggtgttg
     1081 caacaccatc tttaaaagct atacttcttg gagcaccaat gttttcaact tcactatctt
     1141 cttgttttac ttgaaactct ctatgccata aaaccaatct tcttggagta tagaataact
     1201 caaaattaca aagtaaacta tttttttcta aaatatcact ccattttttc tctatatttg
     1261 ataactcctt taaaaatgga atagctggta actcttcaac tccaatttct attaaaagtg
     1321 gtttaagcat tttctttttc cttatcttgg tttttatttt tttctatttc ttctaatttt
     1381 tcaagtttat ctgtatgttt tttaatttgt tgccgattaa aatttataat aaacatagca
     1441 accataaaaa caaatagagc taaaactaca aaatctaaga tatctttcat acttttatct
     1501 ccaataaatc actataaata gctccatcaa tctcttctaa agcaactttt tcaaaatcat
     1561 cactagattt tataatagtc aaaatttttg aatcccacat ataattatca gctatttttt
     1621 gtaatttttt agctaattca ttttcacaaa ttatatattt tgcattaaaa tttgaagcat
     1681 aaatagcctc ttttatagat ttaacatata ccaaaaaatt taaatgttga aaaaaagagt
     1741 attttaaaag ttcttcatta aaatcaaaaa aaatcattga atttggtttt gtatttttaa
     1801 tatcttcaat acttaaaact ttagaaaaag cttcaaatgg aattaattta tctccaatta
     1861 tcttcatttt aagccttttt gttatttaaa caatcttttg aacaaaaaaa ctttccacta
     1921 cttacaatag cctctttttg tgaaacaaaa gtactacaag taggacactc taccatctca
     1981 tcagagatta atttatcatt ttttttaaca ccatcaactc ttttgttttt aaaaaatagc
     2041 aaatatacaa caaaaccagc caaaacaaca gctattactt ttatcaacat ttttttcctt
     2101 taatatataa ataatttcgt tcacatcttg aaacaacttt atagttagat attttagaag
     2161 tttcaatctc atcttctagc atacttcctt tataaaaaag ataagatgta taatcttttt
     2221 ttatcttgga agtgatattt aaaagtaaat ttgtatttgt aacagctcta cttgttatta
     2281 aatcaacttc caaaccttct acattttctg ctcttttttg taaaattgta agattattca
     2341 aacccaaact ggctttcaca aagtttaaaa aagcaactct ttttactctt ggttctatta
     2401 aatacgattt tacatctttt cttgcaattg ctaaaataag tccaggataa ccagctcctg
     2461 ttccaatatc tgcaaaacta ttaaattcat ctataaagtt aagaggataa agtgaatcta
     2521 aaatattttc atagatatca tcatcactca atctaccact taaattatga acct
//
LOCUS       DAOQAN010000110         2563 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_57571_length_2563_cov_2.631579, whole genome shotgun sequence.
ACCESSION   DAOQAN010000110 DAOQAN010000000
VERSION     DAOQAN010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57571_length_2563_cov_2.631579"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="PKO10_02570"
     CDS             complement(<1..520)
                     /locus_tag="PKO10_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HNW66069.1"
                     /translation="MKKINKVLIANRGEIALRIIRACKELEIKSVAVFSEVDVDGLWV
                     KKADECYPIMGDVVQAYLDYEKIISIAKKSDCDAIHPGYGFLSENADFARACEANGII
                     FIGPKPEHIELFGDKMASKVAMKKVGVPVLEGTDEPIIDIEEGAKIAKQIGFPVIIKA
                     AFGGGGRGMRIVR"
     gene            complement(684..1034)
                     /locus_tag="PKO10_02575"
     CDS             complement(684..1034)
                     /locus_tag="PKO10_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HNW66070.1"
                     /translation="MSGIFNKDVIKEKIIENLKKVYDPEIPVDIYSLGLIYNIELEER
                     ENYLFCEIDMTLTSPACPVADSLLEQVRYVAMAVNEVDEAKVNLVFEPVWEPHMMSEE
                     AKEVMGASGAAISW"
     gene            complement(1050..1457)
                     /locus_tag="PKO10_02580"
     CDS             complement(1050..1457)
                     /locus_tag="PKO10_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HNW66071.1"
                     /translation="MSIEKRLENIKEDLDFFDEELAKYEYIIDLGKKLPAFDEKNQIP
                     ENLVHGCTSQVWLICEKKDDKLIFKGTSDAIIVKGLVYIILEIFSNSTIEELKNVDMD
                     IVKDLGLSEVITPNRQSGVIGMIKKIKEYALKA"
     gene            complement(1461..2489)
                     /locus_tag="PKO10_02585"
                     /pseudo
     CDS             complement(1461..2489)
                     /locus_tag="PKO10_02585"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
BASE COUNT          821 a          495 c          264 g          983 t
ORIGIN      
        1 ctcttactat tctcattcct cttccaccac caccaaatgc agctttaatt ataactggga
       61 atccaatttg ttttgcaatt tttgcaccct cttcaatatc aataattggc tcatcagtac
      121 cttcaagaac tggaactcca acttttttca tagccacttt tgatgccatt ttatcaccaa
      181 aaagttctat atgctctggt tttggaccaa taaaaataat accatttgct tcacaagctc
      241 ttgcaaaatc agcattttca cttaaaaatc catatccagg gtgaattgca tcacaatcag
      301 attttttagc tattgatata attttttcat agtctaaata tgcttgaaca acatctccca
      361 taattgggta gcattcatct gcttttttta cccaaagacc atctacatca acttcagaga
      421 ataccgcaac acttttaatt tcaagctctt tacaagctct gattattcta agtgcaatct
      481 ctcctctatt tgcaataagt actttattta tctttttcat atactcacct tttaaaattt
      541 tatattgtga ttttagggta tttttttgat ttattcttct taatttttgt taatttaata
      601 gttggatatt gctaatattg tttaataaaa aaatctttag ctaacaaaac taaagatttt
      661 ttaatttaat tttttaattt atattaccaa gaaatagcag ctccactagc tcccataacc
      721 tcttttgctt cttcactcat catatgaggt tcccaaacag gttcaaaaac tagatttact
      781 tttgcttcat ctacttcatt gacagccata gcaacatatc ttacttgttc aagtaaacta
      841 tctgcaactg gacaagctgg gcttgtaaga gtcatatcta tttcacaaaa tagatagttt
      901 tctctctcct ctaactctat attataaata agtcctaaac tataaatatc aacagggatt
      961 tcagggtcat aaactttttt taaattctct ataattttct cttttattac atctttatta
     1021 aaaattccac tcataattta tccttttgct taagctttta aagcatactc ttttattttt
     1081 ttaatcattc caataactcc actttgtcta tttggagtta taacttcgct aagtcctaga
     1141 tcttttacta tatccatatc aacatttttt agctcttcta ttgtagagtt tgagaaaatc
     1201 tctaaaataa tataaactaa accttttacg attatcgcat cactagtgcc tttaaaaatt
     1261 agtttgtcat cttttttttc acaaatcaac caaacctgtg aagtacatcc atgtactaaa
     1321 ttttcaggaa tttggttttt ttcatcaaaa gctggtagtt ttttccctaa atcaataata
     1381 tattcatatt ttgccaactc ttcatcaaaa aaatctaaat cctcttttat attctctaat
     1441 cttttttcta tactcatttt ttaacccttc aacatactta atgctttttt cagttcaaca
     1501 ataagcttat ctatatcttc aaaatcattg taaaatgcaa ggcttactct aattgttcct
     1561 tttatcccaa gttttttcat aattggttga gcacaatgat gaccaactct aagtgcaact
     1621 tttaatttat caagaagtat tgaaatatca tcatgcataa tacccttgaa gttaaagctt
     1681 ctagttccac tacaatcttt taaatcgtta taaaaaatta tatcaggaag tttttttaac
     1741 tcactatcaa gatactctaa aagctctttt tttctacttt gtattgtttc atatccaata
     1801 ttttcaatat attttaaagc ctctttaaaa gcaatcacac cagcaatatt ttgtgttcct
     1861 gcttcaaatt tataaggtga gttcaaaaaa gtagttccct ctttaaaatc aacactattt
     1921 ataactgctc cacctgtttg ataaggtcta acactttcaa aaaacttctc ttttatataa
     1981 atagctccaa ctcctgttgg accaaaagtt ttatgtcctg agattgcaaa aaagtcacaa
     2041 tcaagctctt gtacatctat tttaaagcta cttaaacttt gagctccatc aatcataact
     2101 acacagccat aatcatgagc catagagatt atttttttaa tatcatgaac tttaccaaaa
     2161 gcatttgtaa catgagcaac acttacaaaa gagtttggat tctttttcaa aatctcttca
     2221 aaatcttcaa aatcaaaatc taagttttcg ttacatttta caacttcaag accagagttt
     2281 aaaaatcttc cttgcatatg ccaaggtaca atatttgcat gatgctctaa gcttgagata
     2341 ataactgttt taaacatata tgcaaaagat gaagctatga aatttatact ttcagttaca
     2401 ccttttgtaa aaattatctc ctcttttttt cttgcattta taaatttttg taaaacttct
     2461 cttgtatttt caaactcagt tgtggcatga gcagcatctc caaaaccatc ttgggaacca
     2521 aggaacgtaa caagcggcgt ttactattac cgtttgaaaa cag
//
LOCUS       DAOQAN010000111         2559 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_57699_length_2559_cov_2.785144, whole genome shotgun sequence.
ACCESSION   DAOQAN010000111 DAOQAN010000000
VERSION     DAOQAN010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57699_length_2559_cov_2.785144"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..142
                     /locus_tag="PKO10_02590"
     CDS             <1..142
                     /locus_tag="PKO10_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HNW66072.1"
                     /translation="SAASIPMAMNYAFEQGRIKSGDTVLFDAFGGGLTWGCALFKFSP
                     KK"
     gene            complement(159..593)
                     /locus_tag="PKO10_02595"
     CDS             complement(159..593)
                     /locus_tag="PKO10_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66073.1"
                     /translation="MPQNITENLEFQDLIQNHCKEQLNFFISKGIDFSIVANISNITF
                     NPELPNSIKENLSKFSLFTLTGYTFQSAHIKEECLYFEAGFGKDNFGSLVQIPFSSIF
                     QIIKNDNIVFINISATLENINQNPKNKSMNIFKNNPNNSKFN"
     gene            complement(605..>2559)
                     /locus_tag="PKO10_02600"
     CDS             complement(605..>2559)
                     /locus_tag="PKO10_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HNW66074.1"
                     /translation="VYEDGLVGEIQKTIDKLLDEKFYPSIQFQTILNKQLKRARYPME
                     VAITGQFSAGKSTFLNALLSRNILPTGITPVTSKVNFINYGEDFKLKITYYSGATEFA
                     PIESIADFTDQRKDEMQNIKYLTLYAPMPILKDISFVDTPGLNSQSQSDTDVTKRVLK
                     DVGGIIWLTLIDNAGKLSEAQVLEEYMQHFKNKSLCVLNQKDKLTPEQVETTTNYVKD
                     KFGKYFAKVVPISAIKALEGRAKEKDILIEDENINLLSELKKELLSSDIENNNNIDKL
                     LSEHKNRIKTIKLADTTLNTKLLEDSNIQEVLDFIETTIRPQASEAKEFAIKKDLRNF
                     CDILVKEYETIIKVYDALVEILESCEPRVLEAFDNIYKKYSKELFTIYNSLESVMEKI
                     AHETFKNIKRKKSTRFEASKSLFGEKIEKFDFETFWVDSDAIYKALFYDDQTVDKMFK
                     RSIKLLKNVELDTDESFREVFRIVKNDIVKWQENYELIRKNREIASDIEFSNTRHFAA
                     KVYENVIRFFYKAILENISALRKKFAYFNGALSYSYIQTTQATIAHFEQQIVESEELY
                     KKEPSRFSINCPREDEIVAKLKANFGFEKIEDFLTSKRNYLFKIIKYSKEQYLEINKD
                     RILFVKDKKEQFITKIEELEKIKNEI"
BASE COUNT          817 a          357 c          294 g         1091 t
ORIGIN      
        1 atcagctgcg tcaattccaa tggctatgaa ttatgctttt gaacaaggta gaattaaatc
       61 tggagacact gtactatttg acgcatttgg tggtggacta acttggggat gcgcactatt
      121 taagttttca cctaaaaaat agttatttaa actatttttt agttaaattt actattattt
      181 gggttatttt taaatatatt cattgatttg ttttttggat tttgattgat attttccaaa
      241 gttgcactta tatttataaa aacaatatta tcatttttaa taatttgaaa aatcgaacta
      301 aatggtattt gaactaagct tccaaagtta tctttcccaa atccagcttc aaaatataaa
      361 cactcttctt taatgtgtgc tgattgaaat gtatagccag ttaaagtaaa taaagagaat
      421 ttactcaaat tctcttttat actatttggt agctcaggat taaaagttat atttgaaata
      481 ttcgcaacaa tactaaaatc aatacctttt gatataaaaa agttaagttg ttctttacaa
      541 tgattttgta ttaaatcctg aaactctaaa ttttctgtaa tattttgtgg caaattatat
      601 ccttttatat ctcattttta attttttcaa gctcttctat ttttgttata aattgctctt
      661 ttttatcttt tacaaaaagt attctatctt tatttatctc taaatattgc tcttttgagt
      721 attttataat tttaaataga tagtttcttt ttgaagttaa aaaatcttct attttttcaa
      781 aaccaaaatt tgcttttaat tttgctacta tttcatcttc tcttggacaa tttatagaga
      841 atctactagg ttcttttttg tataactctt cactttctac tatttgttgt tcaaaatgag
      901 caatagttgc ttgagttgtt tgaatatatg aataagataa tgccccatta aagtatgcaa
      961 acttttttct taaagctgaa atgttttcta aaattgcttt atagaaaaac cttataacat
     1021 tttcataaac ttttgcagca aagtgtcttg tatttgaaaa ctcaatatca gatgcaattt
     1081 ctctattttt tcttataagc tcatagtttt cttgccattt tacaatatca ttttttacta
     1141 ttctaaaaac ttctctaaaa gattcatcag tatctaattc aacattttta agtagcttta
     1201 ttgatctttt aaacatttta tcaacagttt gatcatcata aaataaagct ttgtaaatag
     1261 catcactatc tacccaaaat gtttcaaaat cgaatttttc tattttttca ccaaatagtg
     1321 attttgaagc ttcaaatctt gtagattttt ttctttttat atttttaaat gtttcatgtg
     1381 caattttctc cattacactc tctagagagt tatagattgt aaacagttct tttgaatatt
     1441 ttttatagat attatcaaaa gcttctaaaa ctctaggttc acaagactct aaaatttcaa
     1501 ctaaagcatc ataaactttt attattgttt catactcttt tactaaaata tcacaaaaat
     1561 ttcttaaatc tttttttata gcaaactctt ttgcttcaga agcttgtggt ctaatagtag
     1621 tttcaataaa atctaaaact tcttgaatat ttgagtcttc caaaagtttt gtattaagtg
     1681 ttgtatcagc taattttata gtttttattc tatttttatg ttcagataaa agtttatcta
     1741 tgttgttatt attttcaata tcactagata aaagctcttt ttttaactca gataaaagat
     1801 ttatattttc atcttctatt aaaatatctt tctcttttgc tcttccttct aatgctttta
     1861 ttgcagaaat tggcacaact tttgcaaaat atttaccaaa tttatctttt acatagtttg
     1921 tagttgtttc tacttgttca ggagttagtt tatctttttg atttaaaaca cacaaagatt
     1981 tatttttaaa gtgttgcata tactcttcta aaacttgtgc ttctgatagt tttccagcat
     2041 tatcaataag tgttaaccaa ataattcctc caacatcttt taatactctt tttgtaacat
     2101 ctgtatcact ttgtgattga gagttaagac caggagtatc aacaaaagat atatctttta
     2161 aaataggcat tggagcataa agagtaagat attttatgtt ttgcatctca tcttttcttt
     2221 gatctgtaaa atcagcaata ctttctattg gtgcaaactc tgttgctcca gagtagtatg
     2281 taattttaag tttaaaatct tcaccataat ttataaaatt tactttagag gtaactggag
     2341 taattccagt tggtaaaata tttcttgaga gtagggcatt taaaaatgta gattttccag
     2401 cactaaattg tccagtaatt gcaacttcca taggatatct agctcttttt aattgtttat
     2461 ttaaaattgt ttgaaactga attgatggat agaatttttc atctaaaagt ttatctattg
     2521 ttttttgaat ttcaccaact agaccatctt catatacta
//
LOCUS       DAOQAN010000112         2551 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_57971_length_2551_cov_3.361779, whole genome shotgun sequence.
ACCESSION   DAOQAN010000112 DAOQAN010000000
VERSION     DAOQAN010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57971_length_2551_cov_3.361779"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="PKO10_02605"
     CDS             complement(<1..501)
                     /locus_tag="PKO10_02605"
                     /inference="COORDINATES: protein motif:HMM:NF018218.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HNW66075.1"
                     /translation="MMNKQQNNNPNNNQNNNNNFFNNNPLLIFVAFSLVTIFAFKAIF
                     PDSETNATNSNIQAYGSNVSKTITYSELKKLIVSGKIEYVGIGNTLIRAVSKNDGMQR
                     VTYTARRVVPDETLIPELEKSGISYGGISEENLLSDILFGWVLPIFLFLLFGCFWLKE
                     CKNLWVV"
     gene            complement(516..1322)
                     /locus_tag="PKO10_02610"
     CDS             complement(516..1322)
                     /locus_tag="PKO10_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HNW66076.1"
                     /translation="MSKEYFELLIKPEDNYELFLNLILSLTDDAIEENDGEIIVRSED
                     SLEDFQEPLEKFAKALNTKCYIKCEKKESIDWIKKYQDSIQAVEVGSFYVRPSWSEAK
                     EGKIDILIDPALAFGSGHHETTSSCLLAIDEFVKKDDSLLDVGTGSGILAIAAAKKGA
                     VVDVCDTDEICIESTKSNFALNNQEINKSWIGSVNFAKEKYDVVLANIIADVLVMISD
                     DLINSTKDGGIIIVSGILDKHEKRVLSKFSNLKELKIIHKNEWVTIVFKK"
     gene            complement(1392..2324)
                     /locus_tag="PKO10_02615"
     CDS             complement(1392..2324)
                     /locus_tag="PKO10_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008337528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HNW66077.1"
                     /translation="MKKKLDFSKPLIVLAPLAGYTDLPFRSVVKKFGADLTISEMISS
                     NALVYKSARTLKMIEKSPSEDPYFVQIAGNSAQLVREAVEILNDVEGIDGIDLNCGCP
                     APKVFSHGSGSNLLGDLKKLEEILSTIKKYSKKEYTSAKVRLGVNEKIPVEIGKAVEA
                     CGVDFVSVHGRTRAGKYKAPVDYDAIKQMKEAISIPVIANGDIKDYKKAKEVLEYTKA
                     DGVMIGRGAIGKPWVFYQLKHELEDIDNSIKKEIILEHYDKMLEFHGDYGAIIFRKLL
                     HAYSKGYTGANEFRDLVNQISDKNIMRDLIESFF"
     gene            complement(2327..>2551)
                     /locus_tag="PKO10_02620"
     CDS             complement(2327..>2551)
                     /locus_tag="PKO10_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66078.1"
                     /translation="ISIKQNELSVLEKISQEQEEATVAYLYILSEFEMKDKLRENLAN
                     REGNDFAPFKALIELKDAGKHYSLESLSYN"
BASE COUNT          827 a          477 c          256 g          991 t
ORIGIN      
        1 caccacccat agatttttgc attcttttaa ccaaaaacat ccaaatagca aaaataaaaa
       61 tattggtaaa acccatccaa aaagaatatc agatagaaga ttctcttcgc ttattccacc
      121 ataagaaata ccactttttt ccaactcagg aattaatgtc tcatcaggaa caactcttct
      181 tgctgtataa gtaactcttt gcattccatc atttttacta acagctctaa ttagagtatt
      241 tccaatacca acatactcaa tttttccact tacaataagt ttttttaact cagaatatgt
      301 aattgttttg cttacattac ttccataagc ttgaatattt gaatttgtag catttgtttc
      361 actatcagga aaaattgctt taaatgcaaa aattgttaca agcgaaaatg caacaaatat
      421 caaaagtgga ttattgttaa aaaaattatt gttattattt tgattattgt ttggattgtt
      481 attttgttgt ttattcatca ttatttatat ccctcttatt ttttaaatac tattgttacc
      541 cactcatttt tgtgaataat ttttagctct tttaaattag aaaatttact taaaactctc
      601 ttttcatgct tatctaaaat accagaaact attataattc caccatcttt tgtagaattt
      661 atcaaatcat ctgatatcat aacaagaaca tctgcaataa tatttgccaa aactacatca
      721 tatttttctt tggcaaaatt tacagaacca atccaagatt tatttatctc ttgattattt
      781 aaagcaaaat ttgattttgt actctctata caaatttcat ctgtatcaca aacatcaaca
      841 actgcacctt tttttgcagc tgcaattgct aaaataccac ttcctgttcc aacatccaat
      901 aagctatcat ctttttttac aaactcatca attgctaata aacaagaaga agttgtttcg
      961 tggtgtcctg aaccaaaagc aagtgctggg tctattaaaa tatcaatttt tccctctttt
     1021 gcttcactcc atgatggtcg cacataaaag cttccaactt caacagcttg tatagagtct
     1081 tgatattttt ttatccaatc tatactctct tttttttcac attttatata acattttgta
     1141 tttaatgctt ttgcaaattt ttcaagtggt tcctgaaaat cttcaaggct atcttcactt
     1201 ctaacaatta tttcaccatc attttcttca attgcatcat ctgtaagtga taaaattaga
     1261 tttaaaaaca gttcataatt atcttctggt tttattagta attcaaaata ctcttttgac
     1321 aaataaaaat tcctttatat ttttttaaaa actcaatatt ctatctaaat aactttttaa
     1381 tcaaattaaa tctaaaaaaa gctctctatt aaatctctca ttatattttt gtcactaatt
     1441 tgatttacta aatctctaaa ctcatttgct cctgtataac cttttgaata agcgtgtaac
     1501 aattttctaa aaattatagc tccatagtct ccatgaaact caagcatttt gtcatagtgt
     1561 tctaaaataa tctctttttt tatagagtta tcaatatctt caagctcatg ttttaattga
     1621 taaaaaaccc aaggtttacc aatagcacct cgtcctatca taacaccatc agcttttgtg
     1681 tactctaaaa cctcttttgc ttttttataa tcttttatat caccatttgc aataactgga
     1741 attgaaatag cctctttcat ctgtttaatt gcatcataat ctacaggggc tttatatttt
     1801 ccagctcttg ttcttccatg aactgataca aaatcaactc cacaagcttc aaccgctttt
     1861 ccaatttcaa ctggaatttt ttcattaact ccaagcctta cttttgcact tgtgtactct
     1921 ttttttgaat attttttgat agtacttaaa atctcttcaa gcttttttaa atcacctaaa
     1981 agattagaac ctgaaccatg agagaatact ttaggagcag gacaaccgca gtttaaatca
     2041 attccatcaa ttccttcaac atcatttaat atttcaactg cttctcttac aagttgagca
     2101 ctatttccag caatttgtac aaaatatggg tcttctgaag gagacttttc tatcatctta
     2161 agtgttcttg cacttttata aaccaaagca tttgaactta tcatctctga aattgtaaga
     2221 tcagctccaa actttttaac aacacttcta aaaggtaaat ctgtataacc agcaagtgga
     2281 gcaagcacta taagaggctt gctaaaatct aatttttttt tcattcttag ttatacgaca
     2341 agctttccaa actataatgt tttccagcat cttttagttc aattagtgct ttaaaaggtg
     2401 cgaaatcatt accttctcta tttgctaaat tttctcttag tttatctttc atctcaaatt
     2461 cactcaaaat atataaatat gcaactgtag cttcttcttg ttcttgtgaa attttttcta
     2521 atactgacaa ttcattttgt ttgatactaa t
//
LOCUS       DAOQAN010000113         2534 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_58544_length_2534_cov_3.219847, whole genome shotgun sequence.
ACCESSION   DAOQAN010000113 DAOQAN010000000
VERSION     DAOQAN010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58544_length_2534_cov_3.219847"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            82..774
                     /locus_tag="PKO10_02625"
     CDS             82..774
                     /locus_tag="PKO10_02625"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HNW66079.1"
                     /translation="MKKLLTVAILLPILSFSYEINFNKSFSKVVNPDLLTTNINISVE
                     KKDEKSVNIEVEKFNNFLKNTKNITIKNTNYNLTPKYDYENNKSIFKGFIANTRFIIE
                     SKDPKEINNFLADLMALKDSLKSDDIKINISNLSWEIGENLQNKNIDELRVEVLLWIG
                     NYTKELSNKIGKKCEIKNVNINENFDYPTFKNRVMSSSLDMVNSSESINISPINTEEI
                     IKINTNFILDCK"
     gene            771..1322
                     /locus_tag="PKO10_02630"
     CDS             771..1322
                     /locus_tag="PKO10_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008335321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HNW66080.1"
                     /translation="MIVCAGNQETFPFAKSIGVGLVSSAINLTKICLFNRPEFILFIG
                     SAGSYGNHKIFDIVESKNSSNIEMSFLENFSYTPIDNAVRIDTNLTKSEVVVNSSNYI
                     STNQTLSKEFLQYNVELENMEFFSVLSVAKEFEIPAFGIFIVTNYTNEDAHNDFLKNH
                     KIAMEKLTQYLIEKNIIKIKAEE"
     gene            1322..2404
                     /gene="rlmN"
                     /locus_tag="PKO10_02635"
     CDS             1322..2404
                     /gene="rlmN"
                     /locus_tag="PKO10_02635"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HNW66081.1"
                     /translation="MTKEILSSIYDYTLDELKERLKPSFRAKQVYNWLYKKYANSYDD
                     MKNLPKELIEDLKTNYPIDILKVVKKEQSRDGSIKYLFKLRDNHTIEAVLLLMKDKKI
                     DEDGQIVRSEKYTVCISSQVGCKVGCSFCLTARGGFVRNLTVGEYIAQIVHIKRDNEI
                     AENKALNIVYMGMGEPLDNFDNFVKAVEIFSELDGLAISRRRQTVSTSGIASKIKKLG
                     EKDLQIQLAISLHAVDDELRSELIPMNKAYNIASIIEAVKEFPVDTRKKVMFEYLVIK
                     DKNDSLDAAKKLVKLLTGLQAKVNLIYFNPYPGTTYQRPLETDMMRFKDFLNSKGVIC
                     TIRESKGLDISAACGQLKEKELNGNS"
BASE COUNT         1076 a          234 c          392 g          832 t
ORIGIN      
        1 tcaatttttc actttaaaga ggttgatatt atggatttaa aaagatattt acaaaaaaac
       61 aatataaatg taaggatata aatgaaaaaa ctattaacag ttgctatact tttaccaata
      121 ttgagttttt cttatgaaat aaattttaat aaatcatttt caaaagttgt aaaccctgat
      181 ttattaacaa caaatataaa tataagtgtt gaaaaaaaag atgaaaaaag tgtaaatatt
      241 gaagtagaaa aatttaataa ttttttaaag aatacaaaaa atattacaat aaaaaataca
      301 aattataatt tgactccaaa atatgattat gagaataata aatcaatatt taaaggattt
      361 atagcaaata caagatttat tatagagtca aaagatccaa aagagataaa caatttttta
      421 gctgatttaa tggctttaaa agatagctta aaatcagatg atattaaaat aaatatatca
      481 aatttatctt gggaaattgg tgaaaattta caaaataaaa atattgatga actaagagta
      541 gaagttttat tatggatagg aaattataca aaagaacttt caaataagat tggaaaaaaa
      601 tgtgaaatta aaaatgtaaa tataaacgaa aactttgact accctacttt taaaaataga
      661 gtaatgagtt cttctttaga tatggtaaat tcatctgaat caataaatat ttcaccaata
      721 aatacagaag aaattataaa aataaataca aattttatac tggattgtaa gtgattgtat
      781 gtgctggaaa tcaagaaact tttccttttg ctaaatcaat aggtgttgga ttagtttcta
      841 gtgcgataaa tctaacaaaa atttgtctat ttaatagacc agagtttatt ttatttattg
      901 gaagtgctgg aagttatgga aatcataaaa tatttgatat tgttgaatca aaaaactctt
      961 caaatattga gatgagtttt ttagaaaatt tttcatatac tccaatagat aatgcagtta
     1021 gaatagatac aaatttaaca aaaagtgaag tagttgtaaa tagctcaaac tatataagca
     1081 caaatcaaac tttatcaaaa gagtttttac aatacaatgt cgaacttgaa aatatggagt
     1141 ttttttctgt attaagtgtt gcaaaagagt ttgaaatacc agcttttggt atttttattg
     1201 ttacaaacta tacaaatgaa gatgcacaca atgatttttt aaaaaatcat aaaattgcaa
     1261 tggaaaaact aacacaatat ttaatagaaa aaaatataat taaaataaaa gcagaagagt
     1321 aatgacaaaa gagatattat catctatata tgattacaca ttagatgagt taaaagagag
     1381 attaaaacca tcatttagag caaaacaggt ttataattgg ctttataaaa agtatgcaaa
     1441 ctcttatgat gatatgaaaa atcttccaaa agagttaata gaggatttaa aaacaaacta
     1501 tccaattgat atattaaaag ttgtaaaaaa agagcaaagt cgtgatggaa gtataaaata
     1561 cctttttaaa ttaagagata atcacactat tgaagctgtt ttacttttga tgaaagataa
     1621 aaaaattgat gaagatggac agattgtaag aagtgaaaaa tatacagttt gtatctctag
     1681 tcaagttgga tgtaaagttg gatgtagttt ttgtttaacg gcacgtggtg gatttgtgcg
     1741 aaatcttaca gttggagagt atatagctca aattgttcat ataaaaagag ataatgaaat
     1801 agctgaaaac aaggctttaa atattgttta tatgggaatg ggtgaacctc ttgataattt
     1861 tgataatttt gtaaaagctg ttgagatttt ctcagaactt gatggtttag caataagtag
     1921 aagaagacaa acggtttcaa cttcgggaat tgcaagtaag attaaaaaat taggtgaaaa
     1981 agatttacaa atacaacttg ctatttcact tcatgctgtt gatgatgagt taagaagtga
     2041 gcttattcct atgaataaag catataatat tgcatcaatt attgaagctg ttaaagagtt
     2101 tccagtagat acaagaaaaa aagttatgtt tgaatatttg gttataaaag ataaaaatga
     2161 tagtttagat gcagcaaaaa aacttgtaaa acttctaact ggtttacaag caaaagtaaa
     2221 tctaatatat tttaatccat atcctggaac aacttatcaa agacctcttg aaactgatat
     2281 gatgagattt aaagattttt taaattcaaa aggtgtaatt tgtacaatta gagagtcaaa
     2341 aggtttagac ataagtgcag cttgtggaca gttaaaagag aaggagttaa atgggaattc
     2401 ttgaaatagc tttaattatt tttataatag ttgtagtgct ttctagtgct attggatttt
     2461 atattcataa taaagatgat aaaaaaaatg ataaatttga ggagaattaa tatgacagtt
     2521 acaagatttg cacc
//
LOCUS       DAOQAN010000114         2520 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_59099_length_2520_cov_2.809736, whole genome shotgun sequence.
ACCESSION   DAOQAN010000114 DAOQAN010000000
VERSION     DAOQAN010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59099_length_2520_cov_2.809736"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /locus_tag="PKO10_02640"
     CDS             complement(<1..666)
                     /locus_tag="PKO10_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66082.1"
                     /translation="MLRFVIYFFLISILLIGFGFSFLNNFEKQKEQIFTKYQLETKTT
                     SLFIQENLKKYLQTKDIEILNTINSVFNDNFSSISIQKALFSIKEKELIELTNNLDKT
                     LSWEISNIKIDDSLGQIILSTQSDDLQKELLTIEGSIPNEINKNITPSSDIYTFVPSK
                     NFRNLSSLVINFDATNQFDETKSSSAIISFEKDISSFTNNQNENIAPLWFKKLIPIYF
                     NEEA"
     gene            complement(673..>844)
                     /locus_tag="PKO10_02645"
     CDS             complement(673..>844)
                     /locus_tag="PKO10_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNW66083.1"
                     /translation="TKNLYLSLDEYKLENSGKLNDNSNIDEWYINNTENILVVKYSQD
                     KISEDDIKSILK"
     assembly_gap    845..854
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(855..1927)
                     /locus_tag="PKO10_02650"
                     /pseudo
     CDS             complement(855..1927)
                     /locus_tag="PKO10_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510637.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            2013..>2520
                     /locus_tag="PKO10_02655"
     CDS             2013..>2520
                     /locus_tag="PKO10_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HNW66084.1"
                     /translation="MNIILASANRGKIEEFKKLLPNNKVIAYSDILGKFDIPETGVTF
                     KENAIIKASVVNEKLKAKGEKDFIVISDDSGISLPILDGAPGVYSARFASLNASDKEN
                     NEKLISKLNELNVEKTAAFYTACIAIIYKDFTYTVHGFMHGMAINKELGTNGFGYDPL
                     FIPDGFDKT"
BASE COUNT          954 a          355 c          313 g          888 t
ORIGIN      
        1 tgcttcttca ttaaaatata taggaattaa ttttttaaac caaagtggcg ctatattttc
       61 attttgatta tttgtaaatg agctaatatc tttttcaaaa cttataatcg cacttgaact
      121 ttttgtttca tcaaactggt ttgtggcatc aaaatttatc actaaagatg ataagtttct
      181 aaaatttttg ctaggaacaa atgtatatat atcactacta ggagtaatat ttttatttat
      241 ctcatttgga atagaacctt caatagtcaa aagttctttt tgtaaatcat cactttgagt
      301 agataaaata atttgtccta aactatcatc tatttttata tttgatatct cccaagatag
      361 agttttatct aaattatttg taagctctat aagctctttc tcttttattg aaaaaagagc
      421 tttttgaata gatattgatg aaaagttgtc attaaaaaca gaatttattg tatttaatat
      481 ctcaatatct ttagtttgta gatatttttt taaattctct tgaataaaaa gagatgtagt
      541 ttttgtctct agttgatatt ttgtaaaaat ttgctctttt tgtttttcaa aattatttaa
      601 aaatgaaaac ccaaatccta ttaataaaat agatattaaa aaaaagtaaa ttacaaatct
      661 taacatctta aatcacttca aaattgattt aatatcatct tcacttatct tatcttgact
      721 atattttact actaaaatat tttctgtatt gtttatatac cactcatcga tatttgaatt
      781 atcgtttagt tttccactat tttcaagttt atattcatct aaacttaaat ataaattttt
      841 tgttnnnnnn nnnnatctca taagagtcta atttgtctaa taatatacca ccaaaaactc
      901 caccaacaaa tgttcctaaa tatccaaaag agttaaatac tcctaaaaca ctacctcttt
      961 gatgaacttt tgcaaattta gatgtaagag attgcataat tggttcatgc atattaaagc
     1021 ctataaaaaa tagaacaaca ccaatacaaa agattataga actactgcta aatcccatta
     1081 ataaataaga gattataaat aataaaattc ctataactaa tacttctcta tatttgcctt
     1141 ttttctctgc taaaattgaa gctggtccca ttgcaagaag ccccaaaatc atagatggaa
     1201 tataaacttg ccataaatct tttatctccc aactgtatgt ttttgtaaga attattggaa
     1261 taactaaaaa tgcaaaagtc attaatgctt tttgtaaaaa gttagtaatg tgcattttat
     1321 ttatatttgc atttcctaaa acatctttta ttctcaattt atcattatat gtatgagtta
     1381 tttttggtgg atttggtact tttttaacta atataaaaat agcaatcaaa gataaaatca
     1441 tagtaatata aaaaagagtt ggaacacctg catatccacc aataactgga cccaatccca
     1501 tagcaagtgc aaatgaaata ccaataaaac cacccataat tgccatggct tttgatcttt
     1561 gttcttcttt tacaacatca ctaatcatag cagttaccac tgctccaata gctccagcac
     1621 cttgtaaaag tcttcctaaa agaagagcta aaatatctgt tgctagagca caaataagtg
     1681 aaccaatagc aaaaagtaaa agaccagtga ttattgtgcc ttttctccct aatttgtcac
     1741 tcataattcc aaaaggagtt tgaaaaataa cttgagttaa agcataacca ccaacaacaa
     1801 ttcctacaag tgtagcattt gcaccttcta ggctaaatgc atatacagag ataacaggaa
     1861 gaactataaa aagtccaaaa aatcgtaaag atataataaa actcaaaggt aaaactgatt
     1921 taatcataca taatcctaaa tatattataa tttgagtatt gattatatac taaggttcct
     1981 tttaaatagg aactattttt taaaggcaaa aaatgaatat tattttagca agtgcaaata
     2041 gaggtaaaat agaggagttt aaaaagcttc ttccaaataa caaagttatt gcatatagtg
     2101 atattttagg aaaatttgat attcctgaaa caggagttac atttaaagag aacgcaatta
     2161 taaaagcaag tgtagtaaat gagaaattaa aagcaaaagg tgaaaaagat tttatagtta
     2221 tttcagatga ttctggaata tcacttccta ttttagatgg agcacctgga gtttatagtg
     2281 caagatttgc aagtttaaat gcaagtgata aagagaataa tgaaaaatta atctcaaaat
     2341 taaatgagct taatgtagaa aaaacagcag ctttttatac agcttgcata gcaataatat
     2401 ataaagattt cacatataca gttcatggtt ttatgcatgg aatggctata aataaagagt
     2461 taggaacaaa tggttttgga tatgacccac tttttattcc agatggtttt gataaaactt
//
LOCUS       DAOQAN010000115         2515 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_59284_length_2515_cov_2.070732, whole genome shotgun sequence.
ACCESSION   DAOQAN010000115 DAOQAN010000000
VERSION     DAOQAN010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59284_length_2515_cov_2.070732"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="PKO10_02660"
     CDS             complement(<1..330)
                     /locus_tag="PKO10_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HNW66085.1"
                     /translation="MNLNDFDITTHSGLYISKDEHPIFGKKYIARFQYDKKRYVKVLG
                     YEKRDKITLTTAINLIEEFKASIFKKSEEVEIKDNKKSVAKSVSKNSNTNSDELKKLK
                     EENSYLKS"
     gene            complement(327..1226)
                     /gene="ppk2"
                     /locus_tag="PKO10_02665"
     CDS             complement(327..1226)
                     /gene="ppk2"
                     /locus_tag="PKO10_02665"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HNW66086.1"
                     /translation="MNFEKFYEKSRRANANMSLDEFIEKLKEANGSFVSDLHSKFIYR
                     TKDDILKPYQVELIKLQAHLEKHNEKMIILMEGRDASGKGGAIRRITRYMNEKHYRVV
                     ALGKPSDVQKTQWYFQRYVEQFPKGGELVIFDRSWYNRAGVEPVMGFCTKEEYKQFLT
                     DVPDFEKMLVESGIVVLKYYFSVSKKEQEKRFKKRKIDPLKEYKLSPIDQEAQKLWDK
                     YTDAKYKMLMSTHTPISPWTVIKSDNKKSARLNCIRHILSTIDYSKKTKDEQITKIDR
                     DIVINGAVEIEKMKEKRENGRDK"
     gene            complement(1265..1579)
                     /locus_tag="PKO10_02670"
     CDS             complement(1265..1579)
                     /locus_tag="PKO10_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HNW66087.1"
                     /translation="MSVLMSLAMFPTDTKGSKSKEVAQVLEVIKSSGLDYQLTSMNTI
                     VEAKTLKELLDLLNLCYLKLEELGCNRVYAVANFDIRTSGENRIETKIKSVEKHIGKV
                     SK"
     gene            1652..1993
                     /locus_tag="PKO10_02675"
     CDS             1652..1993
                     /locus_tag="PKO10_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66088.1"
                     /translation="MKLDVTAEVILSQLGYSNNEASLKQAQKAIDVTKGYEKFAKQII
                     TLNDHLKKMNAYVGLSNKTDFFKIKCDENDSKEIIEEFHDAVWKWAEKYNVELERLDK
                     KPIYYILGVSN"
     gene            complement(2043..>2515)
                     /gene="msrA"
                     /locus_tag="PKO10_02680"
     CDS             complement(2043..>2515)
                     /gene="msrA"
                     /locus_tag="PKO10_02680"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HNW66089.1"
                     /translation="AYFAAGCFWGVEFFFQKQIGVLEVVSGYMGGDIENPTYEIVCSG
                     FSGHLEVVEVIFDESIVSYETLAKLFFEIHDFTQTNGQGPDIGTQYLSAIFYCDENQK
                     NIAEELIEQLKKKAYKVATTLRPKVKFYKAESYHQNYYNKSGKTPYCHSYRKVF"
BASE COUNT          801 a          412 c          250 g         1052 t
ORIGIN      
        1 agattttaaa taactattct cttcttttaa tttttttaat tcatcactat ttgtattact
       61 attttttgat actgattttg caactgattt tttattatcc ttaatttcaa cttcttctga
      121 ttttttaaag atagaagctt taaattcttc aatcaaatta atcgctgttg ttaatgttat
      181 tttatctctc ttttcataac ccaaaacttt aacatatctt tttttgtcat attgaaatct
      241 tgcaatatat tttttaccaa aaattgggtg ttcatctttt gaaatataaa gaccactatg
      301 agttgttatg tcaaagtcat ttaaattcat ttatctctcc cgttttctct tttttctttc
      361 atcttttcta tttcaactgc accgttaatt actatatctc tatcaatttt tgttatttgt
      421 tcatcttttg tttttttact ataatcaatt gtgcttaata tatgtctaat acaatttaat
      481 ctagcacttt ttttattatc gctttttata acagtccaag gtgaaattgg tgtgtgtgtt
      541 gacattaaca ttttatattt agcatctgta tatttatccc acaatttttg agcttcttga
      601 tctattggtg ataatttata ctcttttaaa gggtctattt ttcttttttt aaatcttttt
      661 tcttgctctt ttttagatac agaaaaataa tatttaagta ctacaattcc tgattcaaca
      721 agcattttct caaaatcagg aacatctgtt aaaaattgtt tatactcttc ttttgtacaa
      781 aatcccatta caggctcaac tcctgctcta ttgtaccaac ttctatcaaa tattactagc
      841 tcaccacctt ttggaaattg ctctacatat ctttgaaaat accattgtgt tttttgaaca
      901 tcggaaggtt ttccaagagc tactactcta taatgctttt cattcatata tcttgtgatt
      961 cttctaatag ctccaccttt tccagaagca tctcttcctt ccataagtat aatcatcttt
     1021 tcattatgct tttcaagatg tgcttgaagc tttataagct ctacttgata aggttttaat
     1081 atatcatctt ttgttctata tataaatttt gagtgcaaat cacttacaaa actaccattt
     1141 gcctctttta atttttcaat gaactcatct aaactcatat ttgcatttgc tctacgactt
     1201 ttttcataaa acttttcgaa attcattttg cccctttttt atttatcaat atattataca
     1261 caaactattt agaaactttt cctatatgct tttcaactga tttgattttt gtttctattc
     1321 tattttcacc acttgttctt atatcaaaat tagcaactgc atatactcta ttacatccta
     1381 actcttcaag ttttaaataa caaagattta ataaatccaa tagctctttt agagtttttg
     1441 cttcaacaat tgtattcata gatgttaatt gataatctaa tccactactt tttataactt
     1501 ctaaaacttg tgctacttct ttgcttttac ttccttttgt atctgttgga aacattgcta
     1561 aactcattaa aactgacatt ttttatacct tttaaatcat attttcaaat ttgttttgct
     1621 agtatataca aatttcatta aaaggataaa tatgaaacta gatgtaacgg ctgaagttat
     1681 actatcacaa cttggttatt caaacaacga agcttcttta aaacaagctc aaaaagctat
     1741 agatgtaaca aaaggttatg aaaaatttgc aaaacagata attactctaa atgaccacct
     1801 aaaaaaaatg aatgcctatg ttggtttatc aaataaaaca gattttttta aaataaaatg
     1861 tgatgaaaat gattcaaaag agataattga agagtttcat gatgctgttt ggaaatgggc
     1921 agaaaaatat aatgtagaac ttgaaagact tgataaaaaa ccaatttatt atattttagg
     1981 agtttcaaac tagattttct agtttgaaat ttataataat tttttaaaaa tttaactcta
     2041 ttttaaaaaa cttttctata actatgacaa tatggagttt ttccactctt attataatag
     2101 ttttgatgat aactctctgc tttataaaat ttaacttttg gtcttaaagt tgtagcaact
     2161 ttgtaagctt ttttctttaa ttgttcaatt aactcttctg ctatattttt ttgattttca
     2221 tcacaataaa aaattgcact taaatattga gttcctatat ctggaccttg tccatttgtt
     2281 tgggtaaaat catgaatttc aaaaaaaagt tttgccaaag tttcataact tacaatactt
     2341 tcatcaaaaa taacttctac aacttctaga tgtccactaa aaccgctaca aactatctca
     2401 tatgtaggat tttctatatc tccacccata tatcctgaaa ctacttctaa aactcctatt
     2461 tgtttttgaa aaaaaaattc tactccccaa aaacaaccag ctgcaaaata tgctt
//
LOCUS       DAOQAN010000116         2514 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_59313_length_2514_cov_2.121187, whole genome shotgun sequence.
ACCESSION   DAOQAN010000116 DAOQAN010000000
VERSION     DAOQAN010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59313_length_2514_cov_2.121187"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1337)
                     /locus_tag="PKO10_02685"
     CDS             complement(<1..1337)
                     /locus_tag="PKO10_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HNW66090.1"
                     /translation="MAQTSRKNIKITKIKGFIIAKKVVSLIILFIILYFQFTTLISTK
                     DVVGNVGASFANFSFKYFSYLSYIYLLFLIYPLYLINFKKGLDYKDLILNISVVFLFL
                     FISLIFQTLLVENPYERGEIGNILVDSLSPFIGQAGLYIFVLIGFSICLLILFETLNI
                     TIEDLKKLKGRIKLRQNSLKNTNDQKVNNPKIQQKFEQKIYQEKPKSIPKIKEEEKII
                     FENQEFSQEESHEEDGFKILEKTQEEDIFNEITNNNQINNNIIVDELEENAKLLSELE
                     FGKTEKPKDFMLPPTSFFQEAPKENKTKVNEAFIDKKIADLLDKLAMFKIDGDVVRTY
                     TGPVVTTFEFKPAPNVKVSKILSLQDDLAMALKAQTIRIQAPIPGKDVVGIEVPNEDT
                     QTIYLKEMLDSEIFQNSKSPLTMILGKDIVGKPFVTDLKKLPHLLIAGTTGSG"
     gene            complement(1295..2242)
                     /gene="lpxD"
                     /locus_tag="PKO10_02690"
     CDS             complement(1295..2242)
                     /gene="lpxD"
                     /locus_tag="PKO10_02690"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HNW66091.1"
                     /translation="MKLQEILDYLNISQNCDIEISGLNGLKDATSNELSFLESSKYIS
                     SLKDTKAAAVFVNKEFEQDVPSGTIAIVCDEPYINLAKASKLFAPKLIESSGNEPIIG
                     ENTTIMPNVHLGKNVVIGKNCTIMSGSFIADCVIIGNNTIIYPNITIYRDCKIGDDCI
                     IHSGTVIGSDGFGFAHTKDGKYIKIYQNGNVIIGNDVEIGANCTIDRAVFSSTIIEDG
                     VRIDNLVHIAHNCKIGRGSILVTQVGLSGSTTLQPYVIMGGQSATSGHLEIAAFSTIA
                     ARGGVTKSIKEPKKTWAGFPLIEHRDWLKLQGKISKLLK"
     gene            complement(2246..>2514)
                     /locus_tag="PKO10_02695"
     CDS             complement(2246..>2514)
                     /locus_tag="PKO10_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HNW66092.1"
                     /translation="KLIPVLKVNEHKNVIEKDTVLMKFSIDNNLADIDVIARAYHGSI
                     QNVTHDSIIVSATDSSDRIINFIKVMEKFNPLEVVRSGIVAMER"
BASE COUNT          847 a          403 c          287 g          977 t
ORIGIN      
        1 tttccacttc cagtagtacc tgcaattagc aagtgtggaa gttttttaag atcagttaca
       61 aaaggttttc ctacaatatc ttttcctaaa atcatagtta aaggagattt agagttttgg
      121 aaaatctcac tatcaagcat ctcttttaaa taaattgttt gtgtatcttc atttggaacc
      181 tcaattccaa caacatcttt tccaggaata ggagcttgaa ttcttattgt ttgggctttt
      241 aaagccattg ctaaatcatc ttgaagactt agtattttgg aaacttttac atttggagct
      301 ggcttaaact caaaagttgt aacaacaggt cctgtataag ttcttacaac atccccatct
      361 attttaaaca ttgcaagttt atcaagtaaa tcagcaattt ttttatcaat aaatgcctca
      421 tttacttttg ttttattctc ttttggagct tcctgaaaaa aactagttgg tggaagcata
      481 aaatcttttg gtttttcagt tttcccaaac tctaattcac ttagaagttt tgcattctct
      541 tcgagttcat ctacaattat attattattg atttgattat tatttgtaat ttcattaaaa
      601 atatcttctt cttgagtttt ttctaatatt ttgaaaccat cttcttcatg tgattcttct
      661 tgagaaaatt cttgattttc aaaaattatt ttttcttctt cttttatttt tggaatagat
      721 tttggttttt cttgatatat tttttgttca aatttttgtt gaattttagg attgtttact
      781 ttttgatcat ttgtattttt tagactattt tgtcttagtt ttattcttcc ttttaacttt
      841 tttaaatcct caattgttat atttaaagtt tcaaaaagaa tcaaaagaca gatactaaaa
      901 ccaattaaaa caaaaatata taatccagct tgtccaataa aaggtgaaag agaatctact
      961 aaaatattac caatttcacc tctttcataa ggattttcaa ccaaaagagt ttgaaaaatc
     1021 aaagatataa ataaaaatag aaatacaact gatatattta gaattaaatc tttataatca
     1081 agcccctttt taaaatttat aagatacaaa ggataaatta gaaaaagtaa atagatataa
     1141 gataaatatg aaaaatattt aaatgagaaa ttagcaaaac tagctccaac atttcccaca
     1201 acatcttttg tactaattaa agttgtaaat tgaaaatata aaattataaa taaaataatt
     1261 aatgaaacga ctttcttagc gataataaat ccttttattt tagtaatttt gatatttttc
     1321 cttgaagttt gagccaatct ctatgctcaa ttaaaggaaa accagcccaa gtctttttag
     1381 gctcttttat agattttgta actccacctc gagctgcaat agttgaaaat gcagcaattt
     1441 ctagatgccc agaagtagca ctttgtccac ccataataac atatggttgt aaagttgtag
     1501 aacctgatag cccaacttga gtaactaaaa tagaacctcg accaatttta cagttatgtg
     1561 ctatatgaac tagattatca attcttacac catcttcaat aatagtagat gagaaaacag
     1621 ctctatctat tgtacaattt gcaccaattt ctacatcatt tcctattatt acatttccat
     1681 tttgatatat tttgatatat tttccatctt ttgtgtgagc aaaaccaaaa ccatcgcttc
     1741 caataacagt tccagagtga ataatgcaat catctccaat tttacaatct ctataaattg
     1801 taatatttgg ataaattatt gtgttatttc cgataattac acaatctgct ataaaacttc
     1861 cagacatgat agtacaattt ttaccaataa ctacattttt tcccaaatgt acatttggca
     1921 taattgttgt attttctcca attattggct catttccact actttcaata agttttggag
     1981 caaaaagttt actagctttt gctagattta tatatggttc atcacatact attgcaatag
     2041 ttccagatgg aacatcttgc tcaaactctt tgtttacaaa aacagctgct gcttttgtat
     2101 cttttagact acttatatat tttgaactct ctaaaaaact aagctcgtta cttgttgcat
     2161 cttttagacc atttaatcca ctaatctcaa tatcacaatt ttgagaaata tttaaataat
     2221 ccaatatctc ttgtaatttc ataaattatc tctccatagc aacaattcca cttcttacaa
     2281 cttcaagtgg attaaacttt tccataactt ttataaaatt tataattcta tcacttgaat
     2341 ctgttgcaga aactataatt gaatcatggg taacattttg aatacttcca tgatatgctc
     2401 ttgctataac atctatatca gcaagattat tatcaattga gaacttcatt aaaactgtat
     2461 ctttctctat aacattttta tgttcattta cttttagaac aggaattagt ttat
//
LOCUS       DAOQAN010000117         2513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_59325_length_2513_cov_3.582181, whole genome shotgun sequence.
ACCESSION   DAOQAN010000117 DAOQAN010000000
VERSION     DAOQAN010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59325_length_2513_cov_3.582181"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="PKO10_02700"
     CDS             <1..567
                     /locus_tag="PKO10_02700"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II and III secretion system protein"
                     /protein_id="HNW66093.1"
                     /translation="TSSSVTDGGTQRTNYEREDIGLTLKVKPRVSSDTKLTLEINTLL
                     ENLKARSVGVSNPDTLKKEINTTAILSNGESVIIGGLIENKNEIVEEKVPVLGDIPVL
                     GGLFKNESNLNRKNNLVIIVTPYIIQKNQDITYIRDKLTKLKALEERYLEESLSDLKV
                     ELKSKNENKIEDTQALQEKKFKDFFEQQ"
     gene            640..1491
                     /locus_tag="PKO10_02705"
     CDS             640..1491
                     /locus_tag="PKO10_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014475017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HNW66094.1"
                     /translation="MELTNICNLKCSFCPPKLFPNKIMDLNTFDRLNFELKDITNELA
                     YHIVGDPLVLTNLSEYLNISKKHNLKVNITTTANNISQKHYEALLNPTIKQINFSLNS
                     YNANSHKKSFDEYIEPILEFVKFAQESKHEYFINFRIWNLDEQESAKEFNLKVFDRLN
                     NFFSSNLNIDEIYENKPKNIRVARKIFINFDEYFVWPNLKNEVVSQKGFCYGLNSHFG
                     ILSNGTVVPCCLDLDANINLGNIQKSTIKEILQGQRAKEMINGFKNNILKEELCQKCE
                     YRTRFDF"
     gene            1536..2336
                     /locus_tag="PKO10_02710"
     CDS             1536..2336
                     /locus_tag="PKO10_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014475016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HNW66095.1"
                     /translation="MNEIEILQRSIRDFFSSKMLKLALIPLFITMLILYMLFFGVASV
                     GIESLKTVAETSQAGGEVVIDENAPFYFVWLTYFIVFLFKYSITSWIAGFLFYSVGAI
                     FVFHLSIVLTLVIIGFLTPFVVKYLNETSYKNLNLKPYGTILGAFWVFLKAFFMMILL
                     YILFIPLYFIPLINFIALYLPLYYFFHKMLNYDVSSTILSKEEYEKIYSKSSSAFRVR
                     TLLLYFISTIPFVTLFVSIFYIIFLSHAYFIELEKLQKIENKDIKEDI"
BASE COUNT         1006 a          231 c          375 g          901 t
ORIGIN      
        1 acttcaagta gtgtaacaga tggtggaact caaagaacaa actatgaaag agaagatata
       61 ggattgactt taaaagtaaa acctagagtt tcaagtgaca caaaacttac tttagagatt
      121 aatacacttt tggagaattt aaaagcaaga tcagttggag ttagtaatcc tgatactctt
      181 aaaaaagaga taaatacaac agcaatttta agtaatggag agagtgtaat aataggtggg
      241 ttaattgaaa ataaaaatga aattgtagaa gaaaaagtac cagttttggg agatattcca
      301 gttttaggag gactttttaa aaatgagtca aatctaaata gaaaaaataa tctagtaata
      361 attgtaactc catatattat tcaaaaaaat caagatatta catatataag agataaatta
      421 acaaagctaa aagcactaga agagagatat ttagaagaga gtttgagtga tttaaaagtt
      481 gaattaaaat caaaaaatga gaacaaaata gaagatacac aagctttaca agaaaaaaag
      541 tttaaagatt tttttgagca acagtgattt ttagcttatt ttgataaaat cagcaaaaaa
      601 attaaggaaa ataagataaa aaagtttaaa aaagttcata tagaacttac aaatatttgt
      661 aatctaaaat gctctttttg cccaccaaaa ctatttccaa ataaaattat ggatttaaat
      721 acttttgata gattaaattt tgagctaaaa gatattacaa atgagttagc ataccatatt
      781 gttggtgacc ctttggtttt gacaaattta agtgaatatt taaatataag caaaaagcat
      841 aatttaaagg taaatataac aacaacagca aataatattt cacaaaagca ctatgaagct
      901 ttattaaatc ctacaattaa gcagataaat ttttctctaa actcatataa tgcaaactct
      961 cataaaaaaa gttttgatga gtatatagag ccaattttag agtttgtaaa gtttgcacaa
     1021 gagagtaaac atgaatattt tataaacttt agaatttgga atttggatga gcaagaaagt
     1081 gcaaaagagt ttaatttgaa ggtttttgat agattaaata actttttttc ttcaaattta
     1141 aatatagatg agatttatga aaataagcca aaaaatataa gagttgctag aaaaatattt
     1201 ataaattttg atgaatattt tgtttggcca aacttaaaaa atgaagtagt aagtcaaaaa
     1261 ggtttttgtt atggtttaaa ctcacatttt ggaattttat caaatggaac agttgttcct
     1321 tgttgtttgg atttagatgc aaatataaat ttgggaaata ttcaaaaatc aactataaaa
     1381 gagattttgc aaggacaaag agcaaaagag atgataaatg gttttaaaaa caacatttta
     1441 aaagaggagc tttgtcaaaa gtgtgaatat aggacaaggt ttgattttta accatttgta
     1501 ggtagaatat agaaaaatta aataaaagat gaaatatgaa tgaaatagag attttacaaa
     1561 gaagtataag agattttttt agttcaaaaa tgcttaaact tgcactaatt ccacttttta
     1621 taacaatgtt gattttatat atgttgtttt ttggagttgc tagtgttgga atagagagtc
     1681 ttaaaactgt ggctgaaacc tcacaagctg gtggtgaagt tgttattgat gaaaatgcac
     1741 cattttattt tgtatggctt acgtatttta tagttttttt atttaagtac tctattactt
     1801 cttggattgc tggattttta ttttatagtg ttggagctat ttttgttttt catttaagta
     1861 ttgtattaac tttggttatt attggttttt taacaccttt tgttgtgaaa tatttaaatg
     1921 aaactagtta taaaaactta aatttaaaac catatggaac tattttagga gcattttggg
     1981 tatttttgaa agcatttttt atgatgattt tattatatat tttatttatt cctttgtatt
     2041 ttattccact tataaatttt atagctcttt atcttccttt gtactatttt ttccacaaaa
     2101 tgttaaatta tgatgtatct tcaacaattt taagtaaaga agagtatgaa aaaatttact
     2161 ctaaatcttc tagtgctttt agagttagaa cacttctttt atattttatt tctactattc
     2221 cttttgttac actttttgtt tcaatttttt atattatttt tttatcacat gcttatttta
     2281 tagaacttga gaaacttcaa aaaatagaaa ataaagatat aaaagaggat atttagtttt
     2341 ggaaaatata acaaaagcaa taataaaatc aaatattgaa atagcaaaat atataaaaaa
     2401 tagtttaaaa gaagatgatt ttacatatac aaaacagatt ggatatggcg gggataattc
     2461 attaaaaatt gatatttttt ttgaagatat atttataaaa aatctaaaag att
//
LOCUS       DAOQAN010000118         2507 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_59559_length_2507_cov_2.844617, whole genome shotgun sequence.
ACCESSION   DAOQAN010000118 DAOQAN010000000
VERSION     DAOQAN010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59559_length_2507_cov_2.844617"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="PKO10_02715"
     CDS             <1..623
                     /locus_tag="PKO10_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014467938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="permease"
                     /protein_id="HNW66096.1"
                     /translation="LIGNTGNLGIPLGIALFGVESVPYTSIINIANIFFMYTISIYFF
                     ARDKFNFKESIKSIFKIPVIWFAIFALAFNYFEFKMPKEFDFALQMGAYTSLTLQLII
                     FGTYLYSVKVKTIPWKLSLQISFAKHILLPIIGIIVVVNFTNFNPMIASILVMELMMP
                     LAVNNVNFSVVYNTKPTDVAATILVSSAIFIAILHFYIEIIDYFIG"
     gene            628..2220
                     /locus_tag="PKO10_02720"
     CDS             628..2220
                     /locus_tag="PKO10_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014472848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HNW66097.1"
                     /translation="MKKYNFENLTIYFEGEIYKSEEYEKNNSAEILKDLYIKYSFDFV
                     SKLDGVFAFCIYDKIKGLYFCSRDRFGNIPFYYHIKNEKFIFSTSIKDILKELPNLPK
                     LNKIALSKYIQHFSTFGEDTFYCDIFKLEDSSYLIFEPKKDLIKKKYYKINTYKAVND
                     ENIALNDLEEILYSAIEKRLISSPSTLLSGGVDSSLISSIYTKISGRKIDTFSIGYNE
                     YKNYCELQYAKITSNHINSNHHEIIIDKKNYIDSFYNTLEFFDEPHGDSAAVPLNFLL
                     KNVKDFGVNSLLSGEGADELFFGYDNYAKFLKYHNFEKSLSKEQNEFLNEIISALQNN
                     TKESEYLRRVVKKQNIYNSFGEIFNDIQKRKLFVKVPTFKSETPKQDFVDNMSYIDLK
                     LWVSNPVLTKVYKVSKENSLQIHTPFLDNDLLKYVFSIESSIKMGNTNKYLLKKIASK
                     YIPEQIINRTKKGFNSPFNEWLQDEFGSSILDTIIEVNRQTGFFNENYLKQIYTLASS
                     NKFKQHLYSLFVFSLWYKKVYL"
     gene            complement(2284..>2507)
                     /locus_tag="PKO10_02725"
     CDS             complement(2284..>2507)
                     /locus_tag="PKO10_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66098.1"
                     /translation="NNNKSLNCSDIKNKILLDRLKELSMSKDKDEDLKYIFDGNGWVL
                     AIIKKLIYCPNLKKQADEIFIHFVDKNKD"
BASE COUNT          956 a          302 c          301 g          948 t
ORIGIN      
        1 cattaatagg aaatacagga aacttaggaa tccctctagg aattgcttta tttggtgttg
       61 aatcagttcc ttatacaagt attattaata ttgcaaatat cttttttatg tacacaatat
      121 ctatatactt ttttgcaaga gataagttta attttaaaga atcaataaaa tcaatattta
      181 aaattccagt tatctggttt gcaatttttg ctttagcttt taactatttt gagtttaaaa
      241 tgccaaaaga gtttgatttt gcattacaaa tgggagctta tacatcttta actcttcaat
      301 taataatttt tgggacttat ttatacagtg taaaagtcaa aactatccct tggaaactat
      361 cattacagat aagttttgca aagcacattt tactaccaat aatcggtatt atagtagttg
      421 ttaattttac aaattttaat cctatgattg cgtcaatttt agttatggaa ttaatgatgc
      481 cactagcagt taataatgta aatttttctg tagtatacaa tacaaaacca actgatgtag
      541 cagctacaat tttagtatca tctgcaattt ttatagcaat tcttcatttt tatattgaaa
      601 taattgatta ttttatagga taaacatatg aaaaaatata attttgaaaa tttaactatc
      661 tattttgaag gtgaaatata taaatctgaa gagtatgaaa aaaataatag tgctgaaatt
      721 ttaaaagatt tatatataaa atactctttt gattttgtat caaaacttga tggagttttt
      781 gctttttgta tctatgataa aataaaaggt ctttactttt gttcaagaga tagatttgga
      841 aatattccat tttattacca cataaaaaat gaaaaattta tattttcaac ttctataaaa
      901 gatattctaa aagagcttcc aaatttgcca aaattaaata aaattgctct tagcaagtat
      961 attcaacatt tctctacatt tggtgaagat acattttatt gtgatatttt taagcttgaa
     1021 gatagttcat atctgatttt tgaaccaaaa aaagatttaa tcaaaaagaa atattataaa
     1081 attaatacat ataaagccgt aaacgatgaa aatatagctt taaatgactt agaagagatt
     1141 ttatactctg caattgaaaa aaggcttata tcgtctccta gcacactttt aagtggagga
     1201 gttgatagct cacttatctc ttcaatttat accaaaatat ctggtagaaa aatagatact
     1261 tttagtattg gttataatga atacaaaaac tattgtgaac ttcaatatgc caaaattact
     1321 tcaaatcata taaactcaaa tcatcacgaa ataataattg ataaaaagaa ctatatagat
     1381 agtttttata atactttaga gttttttgat gaacctcatg gagatagtgc tgctgttcca
     1441 ctaaattttt tattaaaaaa tgttaaagat tttggtgtaa attcacttct atctggcgaa
     1501 ggtgctgatg agcttttttt tggttatgac aactatgcaa aatttttaaa gtaccataat
     1561 tttgaaaaat ctttatcaaa agaacaaaat gaatttttaa atgaaattat atcagctttg
     1621 caaaacaata caaaagagag tgaatattta agaagagttg taaaaaaaca aaatatatat
     1681 aactcttttg gagagatttt taatgatatt caaaaaagaa agctatttgt aaaagttcca
     1741 acatttaaaa gtgaaacacc aaaacaagac tttgttgata atatgagtta tattgattta
     1801 aaactttggg tttcaaatcc tgttttaaca aaagtttaca aagtttcaaa agagaactct
     1861 ttgcaaattc atactccatt tttggataat gatttactta aatatgtttt tagtattgaa
     1921 agttcaataa aaatgggcaa tacaaacaaa tacttactta aaaaaattgc atcaaaatat
     1981 atccctgaac aaataataaa tagaacaaaa aaaggattta actcaccttt taatgagtgg
     2041 cttcaagatg aatttggaag ctctatttta gatactatta tagaagtaaa tagacaaact
     2101 ggatttttta atgaaaatta tttaaaacaa atttatacac ttgctagttc aaataaattt
     2161 aaacagcatt tgtactcact ttttgttttc tctttgtggt acaaaaaagt atatctttag
     2221 attgtttatt taatatatac taaaggctgt tattccaatt tgatattctt gtgcaaccat
     2281 cttttaatcc ttgtttttat ctacaaaatg tataaatatt tcatctgctt gtttttttaa
     2341 attaggacaa tatattagtt ttttaataat agccaatacc caaccatttc catcaaaaat
     2401 atatttaaga tcttcatctt tgtcttttga catacttaac tcttttaacc tatctaataa
     2461 aatcttattt ttaatatcac tacaatttaa acttttatta ttatttt
//
LOCUS       DAOQAN010000119         2500 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_59816_length_2500_cov_2.021268, whole genome shotgun sequence.
ACCESSION   DAOQAN010000119 DAOQAN010000000
VERSION     DAOQAN010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59816_length_2500_cov_2.021268"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..840)
                     /locus_tag="PKO10_02730"
     CDS             complement(<1..840)
                     /locus_tag="PKO10_02730"
                     /inference="COORDINATES: protein motif:HMM:NF024516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3971 domain-containing protein"
                     /protein_id="HNW66099.1"
                     /translation="MKLDKKLILNIENIEYKSEKTMTNSSIEDIKKNLDILPFVLKWF
                     QSIDIEKLSINDNIVKIVLNKDILSVENKFFLLDSKIDVLSKEVLLDINNLYLKDYNI
                     LFKGKAKIDYFDEELKYFGDIYYQDLIVSGNIDITKDRVNFFIKSEFFKNLHFLKKYL
                     DLPEVANFWMYDNVTGDFKLNWFYGEFDLNKNEIIEKSLQGDAVIENAKIRFENSLEE
                     INTTKVNVNFKNSTLHFDLVDAIYKGKELKNSFVTIKDIANENSGLVLVNIEAKTSLD
                     KDIL"
     gene            1004..2014
                     /gene="mltG"
                     /locus_tag="PKO10_02735"
     CDS             1004..2014
                     /gene="mltG"
                     /locus_tag="PKO10_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HNW66100.1"
                     /translation="MLYYLNLPVVTNKIIFIPKGSTSNIVTHLNKNGYEMNIIDEIIL
                     KSGGFIQSGWIDVEQTRLTKMDFLIKLLTSKAALKTITLIPGETSYFFLKKIAEEFNL
                     DENKLQKIYDEHAYKLDGNILADSYSLPVGMSEEYILFYLFSQTNKKYEEFSKKIFGK
                     YDKEKWFRYISLASVIQKEAATTNEMPIIASVVHNRLKKNMALQMDGTLNYGKYSNSV
                     VTADRIRNDETSYNTYKNKGLPKDPVCAVSLDAIKAAIFPVKSNYLYFVRDNKTGLHK
                     FSNDYETHQANINSNIGVAKTYTKVNDKPNDIDNEAIDIMKNDISNQKAPSIKDLFNS
                     VN"
     gene            2133..2500
                     /locus_tag="PKO10_02740"
                     /pseudo
     CDS             2133..2500
                     /locus_tag="PKO10_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509560.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
BASE COUNT          992 a          347 c          288 g          873 t
ORIGIN      
        1 taaaatatct ttatctaaag aggttttagc ctctatatta accaaaacta aaccactatt
       61 ctcatttgca atatctttta ttgttacaaa actattttta agctcttttc ctttatatat
      121 tgcatctact aaatcaaaat gcaaagtact atttttaaaa tttacattaa ctttagttgt
      181 atttatctct tctagactat tttcaaatct tatttttgca ttttcaataa ctgcatcacc
      241 ttggaggctt ttttcaatta tctcattttt atttaaatca aattcaccat aaaaccaatt
      301 tagcttaaaa tctccagtta cattatcata catccaaaag tttgcaactt ctggtaaatc
      361 aagatatttc tttaagaaat gtaagttttt aaaaaattca ctttttataa aaaaattaac
      421 tctatctttt gtgatatcaa tatttccaga aactattaaa tcttgataat aaatatctcc
      481 aaaatacttt aactcttcat caaaatagtc aattttagct tttcctttaa ataaaatgtt
      541 gtaatctttt aaatataaat tattaatatc taacaaaacc tcttttgata aaacatctat
      601 ttttgaatca agtaaaaaaa acttattttc aacacttaaa atatctttat ttaatactat
      661 cttaactata ttatcgttga ttgataattt ttctatatca atactttgaa accattttaa
      721 tacaaatggt aaaatatcta aattcttttt tatatcttca attgagctgt ttgtcatagt
      781 tttttctgat ttatactcta tgttttcaat atttaaaatg agttttttat ccaatttcat
      841 ataaaattgc gaaattttaa aatttaataa ctgaaaagag ctgattttga tacctgaaaa
      901 tagaagagaa aacatcacaa ataagagaga taacgagagt aacaatacaa aaattctgat
      961 cttttttaac actttatatc tatcccttat tttttgtatt gctgtgcttt actatctaaa
     1021 cttaccagtt gttacaaata aaataatctt tattccaaaa ggtagtacaa gcaatattgt
     1081 aacacactta aacaaaaatg gctatgaaat gaatataata gatgagatta ttttaaaatc
     1141 tgggggcttc attcaaagtg gttggattga tgttgaacaa acaagactta caaaaatgga
     1201 ttttttaata aaacttctta cttcaaaagc tgctttaaaa acaattacgc taattccagg
     1261 tgaaacttca tacttttttt taaaaaaaat tgcagaagaa tttaatttag atgaaaacaa
     1321 acttcaaaaa atctatgatg agcatgcata taaattagat ggaaatatat tagcagatag
     1381 ctactctttg cctgttggaa tgagcgaaga gtatatttta ttttatctat tttctcaaac
     1441 aaataaaaaa tatgaagagt tctcaaaaaa aatatttggt aaatatgata aagaaaaatg
     1501 gtttagatac atatctttag cttcagttat acaaaaagag gcagcaacaa caaatgaaat
     1561 gccaattatt gcaagtgttg ttcacaatag attaaagaaa aatatggcac ttcaaatgga
     1621 tggaacatta aactatggaa aatattcaaa tagtgttgtg acagctgata gaattagaaa
     1681 tgatgaaact tcatataata cttacaaaaa caaaggttta ccaaaagatc cagtttgtgc
     1741 agttagtttg gatgcaataa aagctgcaat tttccctgta aaaagtaatt acttatattt
     1801 tgtaagagat aataaaacag gtcttcataa gttctcaaat gattatgaaa cacatcaagc
     1861 aaatattaat tcgaatattg gagtagcaaa aacttataca aaagttaatg ataaaccaaa
     1921 cgatatagat aatgaagcta ttgacataat gaaaaatgat atttcaaatc aaaaagcacc
     1981 ttcaataaaa gatttgttta atagtgtaaa ttaagatgtg aatatttgaa acaatcagat
     2041 attcacacac aaaaatataa ctaaattcaa tatctttaaa tataaatcta gttacaatct
     2101 tgccatatca aatttaaaat ttaggatgaa aaatggcaca aattatttac acaaaagttg
     2161 atgaggcacc agcacttgct acatactcat ttttaccaat tgtacaggct ttcacaaaaa
     2221 gttctggtat acaaatggtt taaaaagata tttcactagc tggaagaatt attgcagctt
     2281 tccctgaaaa cttaaaacca gagcaaaaaa ttggtgatgc tttagctgag cttggtgata
     2341 tgactcaaaa tccagatgca aatattatta aactaccaaa tatctctgca tcaattcctc
     2401 aattaaaagc agctattgct gaacttcaat caaaaggtta taacattcca gattatgact
     2461 caagtgaaga agtaaatgca aaatattcta aaatcttagg
//
LOCUS       DAOQAN010000120         2494 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_60032_length_2494_cov_2.913079, whole genome shotgun sequence.
ACCESSION   DAOQAN010000120 DAOQAN010000000
VERSION     DAOQAN010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60032_length_2494_cov_2.913079"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="PKO10_02745"
     CDS             complement(<1..267)
                     /locus_tag="PKO10_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014473810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNW66101.1"
                     /translation="MSKETVLNKNAFLVSETDAKGIIRFANEDFCNIAGYSLSEMLGK
                     PHNMVRHKDMPKAAFKDLWNTVQKGEIWTGYVKNATKDGGYYWVF"
     gene            complement(280..1515)
                     /locus_tag="PKO10_02750"
     CDS             complement(280..1515)
                     /locus_tag="PKO10_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNW66102.1"
                     /translation="MFGAVSNRDLEEIDKYFQQLIEFLSYEKSEFEYVESTGNKKVDD
                     MFKNWNQQIKKFDKRTKDDMRVLGEIVLTADKVEQGIYKCRIKGDSDNPTISTLRNTL
                     NKMLTSIDDATSRISKVVTSYTNDDFTDYIKVVDNYKDDMKLLMESINLLGKELGNSA
                     KNNFQNGETLENSASTMTNSMNNLAEKANEQAASLEETAAALEEITSITRNNTQNATK
                     MATLGQVVKKSVQTGEELASKTASSMDEINEKVKAINSAITVIDQIAFQTNILSLNAA
                     VEAATAGEAGKGFAVVAQEVRNLANRSAEAAKEIKNLVEEANIKTNDGKLISSDMIEG
                     YKELNKNISETINIIEDVSAASKEQMLGIEQINQTVNMLDRVTQENAFESNQIKEISQ
                     SVSTLAYELLTDAKSKKFN"
     gene            complement(1699..2019)
                     /locus_tag="PKO10_02755"
     CDS             complement(1699..2019)
                     /locus_tag="PKO10_02755"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNW66103.1"
                     /translation="MINILLNAKDAIKNNNIENGEIFISVKKNKSSVEILIEDNGGGI
                     DDKEILKSIFDIYFTTKKESHGTGIGLYMTKIIIEQNLGGKIKAKNQNNGLKFKIKLP
                     ILTI"
     gene            2115..>2494
                     /locus_tag="PKO10_02760"
     CDS             2115..>2494
                     /locus_tag="PKO10_02760"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNW66104.1"
                     /translation="MLSDLSNNIEIFKKGHPVRVYLEENILIKELFSELFNTDPKKDY
                     QKFYNIFNQICEVEKHFARKENQLFPYLEKYGWTGPSQGMWSFHDDIRAIIKDVRATI
                     EAKEFEKIIEKTTNVYNNLVHLINV"
BASE COUNT          780 a          383 c          278 g         1053 t
ORIGIN      
        1 aaaaacccaa taatatccac catcttttgt tgcatttttt acatatcctg tccaaatctc
       61 acctttttga acagtgttcc acaaatcttt aaatgcagct tttggcatat ctttatgtcg
      121 aaccatatta tgtggttttc caagcatttc agaaagagaa taaccagcaa tattacaaaa
      181 atcttcattt gcaaatctta ttattccttt agcatctgtt tcacttacta aaaatgcatt
      241 tttatttagt accgtttctt ttgacatctt attttcctct tagttaaact ttttactttt
      301 tgcatctgtt aataattcat aagccaatgt agaaacactt tgagatatct cttttatttg
      361 atttgattca aaagcgtttt cttgagtaac tctatctagc atatttacag tttgatttat
      421 ctgttcaatt ccaagcattt gctcttttga tgctgctgat acatcttcta ttatatttat
      481 tgtttcagaa atatttttat ttagttcttt ataaccttct atcatatcag atgagattaa
      541 ttttccatca tttgttttta tgttcgcttc ttctactaag ttttttatct ctttagcagc
      601 ttcggcactt ctatttgcca gatttcgcac ctcttgagca acaacagcaa agccttttcc
      661 tgcttctcct gcagttgcag cttcaactgc tgcatttaaa cttaaaatat ttgtttgaaa
      721 tgcaatttgg tcaattactg taattgcact atttatagct tttacttttt catttatctc
      781 atccatagaa gaagctgttt tagaagctaa ctcttcacct gtttgaacag atttttttac
      841 aacttgcccc aaagtagcca ttttagtagc attttgagta ttatttcttg taatacttgt
      901 aatctcttca agcgctgctg ctgtctcttc tagagatgca gcttgttcat ttgctttttc
      961 tgcaagatta ttcatagaat ttgtcatagt tgaagcacta ttttctaaag tctctccatt
     1021 ttgaaaatta ttctttgcac tgtttcctaa ctcttttcca agtagattga tactttccat
     1081 tagaagtttc atgtcatctt tgtaattgtc aacaactttt atatagtcag taaaatcatc
     1141 atttgtataa ctagttacta cttttgaaat tcttgatgtt gcatcatcaa tactagttag
     1201 cattttattt agagtatttc ttaaagtaga gatagttgga ttatcactat cgcctttaat
     1261 acggcattta taaatacctt gctcaacttt atcagcagtt aaaacaattt ctccaagaac
     1321 tctcatatca tctttggttc ttttgtcaaa cttttttatt tgttgattcc aatttttaaa
     1381 catatcatca acttttttgt tacctgttga ttctacatat tcaaattcac tcttttcgta
     1441 agataaaaat tcaattaatt gttgaaaata tttatctatc tcttctaaat ccctatttga
     1501 tactgctcca aacatataaa cacccttttg attgatattt taattaatac tctaattcta
     1561 ttttaataga aaacattttt gtagtttaaa aatgtcattt catagatata aatattatat
     1621 ctacttattt tcggattgta aataaaaaaa gtgttctttt tgtgagtttt ttatatttaa
     1681 tttttatttt tttataattt aaatagttag tattggtaat ttaattttaa attttaatcc
     1741 attattttga ttttttgctt ttatttttcc acctaaattt tgttcaataa ttatttttgt
     1801 catatataaa ccaattcccg ttccatgaga ctcttttttt gtagtaaaat atatatcaaa
     1861 tattgatttt aaaatttctt tatcatctat tccaccgcca ttatcttcta ttaaaatttc
     1921 aactgaagat ttatttttct ttacacttat aaatatttct ccattctcaa tattattatt
     1981 ttttatagca tcttttgcat ttagtaaaat atttatcaat gcttgtttga gtattattta
     2041 ctcttgacta aaatcattat ccaaaagttt taaataggat aaactttatc ctatattaat
     2101 aaaaggaatt taagatgtta agtgatttat caaataatat tgaaatattt aaaaaaggtc
     2161 atccagtaag agtatattta gaagagaata tattaataaa agagctattt tctgaacttt
     2221 ttaatacaga tccaaaaaaa gattatcaaa aattttataa tatttttaac caaatttgtg
     2281 aagtagaaaa gcattttgca agaaaagaga atcaactttt tccttatctt gaaaaatatg
     2341 gttggactgg tccatctcag ggaatgtggt catttcatga tgatattaga gcaataataa
     2401 aagatgtaag agctactatt gaggcaaaag agtttgaaaa aattatagag aaaaccacaa
     2461 atgtatataa taatttagta catttaataa atgt
//
LOCUS       DAOQAN010000121         2486 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_60321_length_2486_cov_2.497738, whole genome shotgun sequence.
ACCESSION   DAOQAN010000121 DAOQAN010000000
VERSION     DAOQAN010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60321_length_2486_cov_2.497738"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            64..624
                     /locus_tag="PKO10_02765"
     CDS             64..624
                     /locus_tag="PKO10_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014473608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HNW66105.1"
                     /translation="MLVGYIRVSTDDQNLSLQKDALLKYGVDERNMFSDKTSGSKDKR
                     VGLDKALEFLKEGDTLVVWKLDRLGRSLAHLISVITDLKNRNVSFVSITEGMDTTTAS
                     GELFFHIFGALAQFERSLIQERVKAGLEAAKNRGIRGGRPRAISEEKMIAIKKALNDG
                     MSKASICRTFEVKRSTLIDSLNRENI"
     gene            complement(858..1511)
                     /locus_tag="PKO10_02770"
     CDS             complement(858..1511)
                     /locus_tag="PKO10_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014475470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNW66106.1"
                     /translation="MDSISKKYGDYKYFSFLEEKDLDKLKDISIKKTYKKDEILFYKG
                     DESKYLYLLVSGIAKLYTHDFKDNEVVIHNLIGPALIAEIMNYEEMDFLANCAFETDA
                     EVILIDYKKFKEEFLQKPEISIFFIKSLTKKIKFLQNFIDYNVSLNSMEKIAKFLYEN
                     EELLKTLKQVKIAQILNITPETFSRQLAKLKKENIIENEKGYIKILDYKKIQNFISN"
     gene            1594..1941
                     /locus_tag="PKO10_02775"
     CDS             1594..1941
                     /locus_tag="PKO10_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012148008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HNW66107.1"
                     /translation="MTHEKYKIEIDVLKKFYELYCIDKHEEQKNILVQLYYKDENFSL
                     ELNLCNKCFEDINYSFNRLQGCPHEIKPRCRNCPSPCYEKTKWKETARVMKYSAIKLS
                     LGKIKSRVMNLFN"
     gene            complement(1943..>2486)
                     /gene="ung"
                     /locus_tag="PKO10_02780"
     CDS             complement(1943..>2486)
                     /gene="ung"
                     /locus_tag="PKO10_02780"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012148009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNW66108.1"
                     /translation="KQNIFKAFYLTKLDNLKVVILGQDPYHGFGQAQGLAFSTPANIK
                     NPPSMQNILKEIQSDLGKKSICEDGDLTPWAKQGVLLLNTILTVEEAKPKSHHNLGWE
                     VFTDNIIKYISDNCEDTIFILWGSPAISKTKLIDRKKHHILTAPHPSPLSSYRGFFGC
                     KHFSQTNNILKSLNKEAIIW"
BASE COUNT          913 a          297 c          335 g          941 t
ORIGIN      
        1 ttatacgata aatactatcg gattgtgata taatacgaaa actatataag agaggttttt
       61 cgtatgctag taggttatat tagagtttca acagatgatc aaaatttatc tttacaaaaa
      121 gatgcacttt taaaatatgg tgttgatgaa agaaatatgt tttctgataa aacatctggt
      181 tcaaaagata aaagagttgg tttagataaa gcattagaat ttttaaaaga aggtgatact
      241 ttagttgtat ggaaattaga tagacttgga agaagtttag ctcatttaat atctgtaata
      301 acagatttaa aaaatagaaa tgtatctttt gtatctatta ctgaaggtat ggatacaaca
      361 acagcttcag gtgaactatt ttttcatata tttggagctt tagctcaatt tgaaagaagt
      421 ttaattcaag aaagagttaa agctggactt gaagctgcta aaaatagagg tattagaggt
      481 ggacgtccta gagctattag tgaagaaaaa atgatagcta ttaaaaaagc tttaaatgat
      541 ggaatgagta aagcaagtat atgtagaaca tttgaagtta aaagaagtac actaattgat
      601 agtttaaata gagaaaatat ttaagctgtc tatttaaata ttatataaaa tatagtttat
      661 aaaaacaaca aagatataaa ttataataat taactggttt ataccaagtt attaaaatta
      721 aatttaccaa cttattttaa aacctcaaaa aaaagagatt ttttatgtga tggcttatat
      781 tatcgaagtg aatggataat aaagtcagta tattttctat aaaaaagtgt atatttttat
      841 ttaataggat ggttatatta attgcttata aaattttgga tttttttata atctaaaatc
      901 tttatatacc ctttttcatt ctctataata ttctcttttt ttagttttgc taactgtctt
      961 gaaaaagttt ctggagtaat atttaatatt tgagctattt ttacctgttt tagagtttta
     1021 agaagttcct cattttcata taaaaatttt gcaatttttt ccatactatt tagacttaca
     1081 ttataatcta tgaaattttg taaaaatttt atcttttttg ttaatgattt tataaaaaat
     1141 attgaaatct ctggtttttg caaaaactcc tctttaaact ttttataatc tattaaaata
     1201 acttcggcat cagtctcaaa tgcacaattt gctaaaaaat ccatctcttc ataattcata
     1261 atttcagcaa taagtgcagg accaatcaga ttgtgaatta caacctcatt atctttaaaa
     1321 tcatgagtat ataactttgc aattccagaa actaaaagat aaagatactt tgactcatca
     1381 cctttataaa ataaaatttc atctttttta tacgtttttt taatagatat atcttttaat
     1441 ttatctaaat ctttctcttc taaaaaacta aaatatttat aatctccata tttttttgaa
     1501 atactatcca tttttctctc cttcttgact gctatcaatt gtctgttaat tattgtatgc
     1561 aaatattata taaagtaaaa ttaaagatat aaaatgacac atgaaaaata caagattgag
     1621 atagatgtac tcaaaaaatt ttatgaactt tattgtatag ataagcacga agagcaaaaa
     1681 aatattttag ttcaactata ttacaaagat gagaatttta gtttggaact taatctttgt
     1741 aataaatgtt ttgaagatat aaattactct tttaatagac tacaaggctg tcctcatgag
     1801 attaaacctc gatgcagaaa ctgtccatct ccatgttatg aaaaaactaa atggaaagaa
     1861 acagcgagag ttatgaaata ttcagctata aaactctctc ttggaaaaat caaatcaagg
     1921 gttatgaatc tttttaatta atctaccaaa taatagcttc tttatttaaa gattttaaaa
     1981 tattatttgt ttgagagaag tgcttacaac caaaaaatcc tctatatgaa ctaagtgggc
     2041 ttgggtgtgg agctgttaaa atatgatgtt tttttctatc tattaatttt gtttttgaaa
     2101 tagcagggct tccccaaaga ataaaaatag tatcttcgca attatcgctt atatatttaa
     2161 ttatattatc tgtaaaaact tcccaaccta aattgtgatg agattttggt tttgcttctt
     2221 ctacagttaa tattgtattt aaaagaagca ccccttgttt tgcccaaggt gttaaatctc
     2281 catcttcaca aatagatttt ttgcccaaat cactctgtat ctcttttaat atattttgca
     2341 tagatggtgg attttttata ttagctggag ttgaaaatgc caaaccttgg gcttgtccaa
     2401 agccatgata tggatcttgt cctaatatta caacttttag attatcaagc tttgttaaat
     2461 aaaaagcttt aaagatattt tgtttt
//
LOCUS       DAOQAN010000122         2484 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_60380_length_2484_cov_2.886785, whole genome shotgun sequence.
ACCESSION   DAOQAN010000122 DAOQAN010000000
VERSION     DAOQAN010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60380_length_2484_cov_2.886785"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            289..819
                     /locus_tag="PKO10_02785"
     CDS             289..819
                     /locus_tag="PKO10_02785"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNW66109.1"
                     /translation="MILVNAALNSDIEEIIKEIKTLSKDIDVKSTQNFVDNYNQFKII
                     KTSSNVISFIAFFLGLLGIVSLMSITVNQRKSEFGIKRALGIKTSKIVYSIIIESFLI
                     GLFSFICAFILSNIVLFFIKNIDSLQGYVNGEISLDLAIYIFVTSILMAIIGSIIPAL
                     NAAKTDPVLLIQGNKI"
     gene            816..1439
                     /locus_tag="PKO10_02790"
                     /pseudo
     CDS             816..1439
                     /locus_tag="PKO10_02790"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            1423..>2484
                     /locus_tag="PKO10_02795"
     CDS             1423..>2484
                     /locus_tag="PKO10_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66110.1"
                     /translation="MEKSINFENFINKHFKISIAFFAIGLFFGIAYSLNLLGFVIDSQ
                     TLNPLNLRAVHISLMLYGFVPLMLSYLPFLLINKEIGISKEGLKYLNIYTQIWYIFLI
                     FMIFSLLLGKNRGLAFYDFTYELNFLLAFAGLFYILALYKFINLYKIVPLWIKVCLQI
                     VIISPIALLILMNPTIGQVESTVSGPHGDNTLGMSLALIPIYYLIIKLLNITEFKARW
                     NILWIIPTIFYFLTVLYRIFFEPLTYNQEWFAQYLTLLYIPLLYRWYKDSNITNVAKR
                     ALLISILAFLFVDIEGNILFIPEIRWLFHRNDLIVAHAHVAMGIGVFFMVIAMFINHI
                     KELHVAKFINIYLVGILGIF"
BASE COUNT          950 a          264 c          343 g          927 t
ORIGIN      
        1 atgacagtag gagcaagtcc tgtagaaaaa ctcccaattg tagctattta tggagttact
       61 caaaatagat ttaaaaatta taaattataa aaaggaaaat atcctttaaa taatgaagtt
      121 atagttggga aatcaatttt tgaacaacta tcaaatagaa atgaagttca aattgcaaat
      181 aaaactttta aaatttctgg tgtttttaaa agtgaaatag gatttgaaaa tggtggaatt
      241 gttttaaata tatcagatgc tggagaaatt ttcaataaat cagcttcaat gattttagta
      301 aatgcagctt taaattcaga tattgaagag attataaaag agataaaaac tttatcaaaa
      361 gatattgatg ttaaatctac tcaaaatttt gtggataact acaatcaatt taaaattata
      421 aaaacatcat caaatgttat atcttttata gcttttttcc ttggattact tggaattgta
      481 agccttatga gtatcacagt aaatcaaaga aaaagtgaat ttggaatcaa aagagctttg
      541 ggtataaaaa cttctaaaat tgtatattca ataataattg aaagcttttt gattggattg
      601 tttagtttta tttgtgcatt tattctttca aatattgttc tattttttat aaaaaatata
      661 gatagtttac aaggttatgt aaatggagaa attagtttgg atttagctat ttatatcttt
      721 gtaacatcaa ttttaatggc aattataggc tcaataattc ctgcattaaa tgcagcaaaa
      781 acagatcctg ttcttttaat ccaaggaaat aaaatatgat aaaagttaaa aatttaacgc
      841 actattataa caatgataag gctcttgaaa atataaattt agagataaat aaaggtgaat
      901 ttatatgttt agttggagag agtggtagtg gaaaatctac tcttttatca ctaatttcaa
      961 ctctattaaa accaacaaaa ggtgaactat tttttgaaaa tttaaattat aaaaatatta
     1021 aagatattga tgattttaga aaaacaaata taggttatat ttttcaattt caatatttaa
     1081 taaaatattt aactgttaaa gaaaatataa aactagctaa caaataggca acagagaatg
     1141 aaattcataa cttactaaaa atcttaagaa tagagaatct atcaaataaa tatccaaatg
     1201 agatttcagg agggcaaaga caaagagttg ctattgcaag ggctttaata aataaaccaa
     1261 aggtaataat tgcagatgaa ccaacaggaa atttagattc aaaaaactct ttaaatgttt
     1321 ttgaaatatt taaaaaacta agtcaagagc aagttactat aatagtggca actcacgata
     1381 aaaatttagc acaaattgca aataaaattt atgaggtaaa agatggaaaa atcaattaat
     1441 tttgaaaact ttataaacaa acattttaaa atatctattg cattttttgc aattggacta
     1501 ttttttggaa ttgcctattc acttaattta ttggggtttg ttattgattc acaaacatta
     1561 aatcctctga atttaagagc tgtacatatt agtttgatgc tttatggatt tgttccttta
     1621 atgctttcat atcttccttt tttacttata aataaagaga ttggtatctc taaagaggga
     1681 ttaaaatatc taaatatcta cactcaaatt tggtatattt ttctaatttt tatgatattt
     1741 tcactacttt taggaaaaaa tagaggttta gcattttatg attttactta tgaactaaat
     1801 tttttattag catttgcagg attattttat attttggctc tatacaaatt tataaatctt
     1861 tacaaaatag ttcctttatg gataaaagta tgtttacaaa ttgtaatcat ttctcctatt
     1921 gctctattaa ttttaatgaa tccaactatt ggtcaagttg aaagtacagt aagtggtcct
     1981 catggagaca acactttggg aatgagctta gctttaattc ctatatatta tttaatcata
     2041 aagcttttaa atattacaga gtttaaagct agatggaata ttttatggat tattccaaca
     2101 atattttatt tcttaactgt tctttataga atattttttg aacctttaac atacaatcaa
     2161 gagtggtttg ctcaatatct aaccctttta tatattcctc ttttatatag atggtacaaa
     2221 gatagtaata tcacaaatgt tgctaaaaga gctcttttaa tttcaatctt ggctttttta
     2281 tttgttgaca tagaaggaaa tattttattt attcctgaaa ttagatggtt gtttcacaga
     2341 aatgatttaa tagttgctca tgctcatgtt gcaatgggaa ttggagtatt ttttatggtt
     2401 atagctatgt ttataaacca tataaaagaa cttcatgtag caaaatttat aaatatatat
     2461 ttagttggaa ttttaggaat attt
//
LOCUS       DAOQAN010000123         2478 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_60592_length_2478_cov_3.413950, whole genome shotgun sequence.
ACCESSION   DAOQAN010000123 DAOQAN010000000
VERSION     DAOQAN010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60592_length_2478_cov_3.413950"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="PKO10_02800"
     CDS             <1..440
                     /locus_tag="PKO10_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014473009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66111.1"
                     /translation="KNSEYKFCINRDFKSVLNQIQNYHKDSWIEENYEKLLINLNNLE
                     NNNSNFKLVSIELYDKNNEKLVSGEIGYMISKTYTSLTGFSSKSKVYNNWGKLQMVLL
                     ALYLEENRFDFWNLGHPYMQYKFDLGAKLYSRDDFLKRWLNSI"
     gene            complement(437..1312)
                     /locus_tag="PKO10_02805"
     CDS             complement(437..1312)
                     /locus_tag="PKO10_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014473007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HNW66112.1"
                     /translation="MKKETLEKRTKIANDIMYYIYTHIETNIDIEELSIDLDISKFHM
                     HRVFKEIFGRNIYESIKSIRLQKASNLLLTNRYSTISSIVNSCGYSSQSSFIKIFKDR
                     FGMSPKEWKNGGYKEYSNEIIKQSKFAMKSKASFDNITPTIVKMKAIESYYIRNRGYN
                     KNIEETWQKLQTWVLTNNIKQYKRAALFHDNPTITPLDECQYIACIIVDDDKNIKSDR
                     IPKFKIAEGVYAKFDLKVKNEDLLPFIQWVYHEWLPRSDYETTTKPSFAIYESVDFIK
                     LDNEYDFSFYVSINY"
     gene            1455..1718
                     /locus_tag="PKO10_02810"
     CDS             1455..1718
                     /locus_tag="PKO10_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HNW66113.1"
                     /translation="MEALLQAIFSFFMIMGIGTSILLVIGFLLKAKGVTFVEFFPKKD
                     KDAPSQVIYNNVISQSPNQGSYGVDARKVAAIMAAIQHHNKKG"
     gene            1724..>2478
                     /locus_tag="PKO10_02815"
     CDS             1724..>2478
                     /locus_tag="PKO10_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002823658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin attachment protein"
                     /protein_id="HNW66114.1"
                     /translation="MSKKYIDIMDTTFRDGFQSVFGGRVLMNDFFPAVEAAKDAGITH
                     FEFGGGARFQSLFFYLNENAFDMMDKFRAIVGPDANLQTLARGINTVMLDTGSRELID
                     LHAKMFAKHGTTTIRNFDALNDVQNLEYSAQCIKNHGLKHEAVVTLMDLPPNCTGAHD
                     VPFYEKTLRNILDSGLPFDSICFKDASGTSSPNKVFETIKMARKLLGDSTHIRLHTHE
                     TAGVSVACYLAALEAGADGIDLAASPVSGGTSQP"
BASE COUNT          876 a          328 c          362 g          912 t
ORIGIN      
        1 taaaaaatag tgaatataaa ttttgtataa atagagattt taaatctgtt ttaaatcaaa
       61 ttcaaaatta ccataaagat tcttggattg aagaaaatta tgaaaagtta ttaataaacc
      121 taaataattt agaaaataac aattcaaact ttaaattagt tagtattgaa ctatatgata
      181 aaaataatga aaaattagta agtggtgaaa ttggttatat gatttctaaa acatatactt
      241 cactaactgg tttttcttca aaatctaaag tctataacaa ttggggcaaa cttcaaatgg
      301 tactattagc tctttattta gaagaaaata gatttgattt ttggaattta gggcatccat
      361 atatgcagta taaatttgat ttaggagcca aactttattc aagagatgat tttttgaaaa
      421 gatggctaaa tagtatttaa tagtttattg aaacgtaaaa actaaaatca tactcattgt
      481 ccaattttat aaaatcgaca ctttcatata ttgcaaatga tggttttgta gttgtttcgt
      541 aatcacttct tggtaaccat tcatgataaa cccactgaat aaatgggagt aaatcttcat
      601 tttttacttt taaatcaaat tttgcataaa ctccttctgc tattttgaat tttggaattc
      661 tatcgctttt tatattttta tcatcatcaa ctattataca agcaatatat tgacactcat
      721 caagtggagt aattgttgga ttatcatgaa agagtgctgc tcttttatac tgttttatat
      781 tgtttgtcaa aacccaagtt tgaagttttt gccatgtctc ttctatattt ttattataac
      841 ctcgatttct tatataatag ctctcaatag ctttcatttt tacaatagtt ggtgttatat
      901 tatcaaagct agctttagat ttcatagcaa attttgattg ttttataatc tcatttgagt
      961 actctttata tccaccattt ttccactctt ttggactcat tccaaatcta tctttaaata
     1021 tttttataaa agatgattgt gaagagtaac cacatgagtt tacaatacta gaaattgttg
     1081 aatatctatt tgtaagaagt aaatttgaag ctttttgaag cctaattgat tttatactct
     1141 cataaatatt tcttccaaat atctctttaa aaacacgatg catatgaaac ttgcttatat
     1201 ctaaatcaat actaagctct tcaatatcaa tgtttgtttc aatatgagtg tatatataat
     1261 acataatatc atttgctatt tttgttcttt tttctaatgt ctcttttttc ataagataat
     1321 tttatcaaaa attagataaa ataagcacta aacaacaatt tataaagcac caaaaaagaa
     1381 gaagaaaata taaaattttc ctaaaatacg gtactttaaa tcaagtgttg atataaaata
     1441 cttaaaggag aaatgtggaa gccctactac aagctatatt tagctttttt atgattatgg
     1501 gaataggaac ctctatactt ttggttattg gatttttatt aaaagcaaaa ggtgtaactt
     1561 ttgttgaatt ttttccaaaa aaagataaag atgctccatc tcaagttatt tacaataatg
     1621 taatatctca aagtccaaat caaggctctt atggtgttga tgctagaaaa gttgctgcta
     1681 ttatggcagc tatacagcat cacaataaaa aaggttgaaa aatatgtcaa aaaaatatat
     1741 tgatataatg gatactactt tcagagatgg atttcaatct gtttttggag gaagagtttt
     1801 aatgaatgac ttctttccag ctgttgaagc tgcaaaagat gctggaataa ctcactttga
     1861 atttggtgga ggagctagat ttcaatcttt attcttctat ctaaatgaaa atgccttcga
     1921 tatgatggat aaatttagag ctattgttgg tccagatgca aaccttcaaa ctcttgcgcg
     1981 tggtattaat actgttatgc ttgatactgg aagtagagaa ctaattgacc ttcatgctaa
     2041 aatgtttgca aaacatggaa caacaactat tagaaatttt gatgcattaa atgatgttca
     2101 aaatcttgaa tatagtgctc aatgtattaa aaatcatgga ttaaaacatg aagctgttgt
     2161 aacacttatg gatttaccac caaattgtac aggagctcac gatgttcctt tttatgaaaa
     2221 aactctaaga aatatattag atagtggatt accatttgac tctatttgtt ttaaagatgc
     2281 aagtggtaca agtagtccaa ataaagtttt tgaaactatt aaaatggcta gaaaactttt
     2341 aggtgattct actcatatta gacttcatac tcatgaaaca gctggagttt cagttgcttg
     2401 ttatttggct gctttagaag caggtgctga tggtattgat ttagcagcat caccagttag
     2461 tggaggaaca agccaacc
//
LOCUS       DAOQAN010000124         2477 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_60618_length_2477_cov_6.052849, whole genome shotgun sequence.
ACCESSION   DAOQAN010000124 DAOQAN010000000
VERSION     DAOQAN010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60618_length_2477_cov_6.052849"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            185..1492
                     /locus_tag="PKO10_02820"
     CDS             185..1492
                     /locus_tag="PKO10_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011980420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HNW66115.1"
                     /translation="MTQTILDFHLTTTNEKLTPRSGVIILGEYLKGLGLEKLCNSHLP
                     LPLNHKGYNPFEFVYPLVLMLHSGGRVIDDIREIKLDYALKQSLNIKNIPTASGIIKW
                     LKRTGLAGVYGIEAINKTLMERYLKRITLHSDEDLILDIDATVIEAHKVTSKYTYKMV
                     PGYTPILGHINGGYLIASEFRDGNIAPADTNLEFIKKCVQYLPKNKKLKWLRADSATY
                     QAEVFNYCDDNNINFAIGGQLDKSVVKQINQISNWEELSKYEDIGEFIHTMTHTKNAF
                     RMIVIRRNITPMLPHLEDILSDKEKAQYHKERFYVMATNNNTLSKEEIIKLYRQRGET
                     SENKIKEFKNGFNMDYLPSRDFISNALYFQIGTLAYNLFILFKTILDENLQHHTVKTI
                     RYKIYNIAGKVISHSRKTILKVNDEFIEILQRIRYKAHEASIE"
     gene            1811..>2477
                     /locus_tag="PKO10_02825"
     CDS             1811..>2477
                     /locus_tag="PKO10_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNW66116.1"
                     /translation="MPLSNLNQEQKEAILCKSGYNLIIASAGTGKTSTIVGRISHLLN
                     NGVKPNEILLLTFTNKAASEMVSRVSNIFSKQIAKEIMAGTFHSVSYKLLKELEINIT
                     LKQPNELKTLFKSIYEKRVFMDRSDEANPYDGGYLYDMYSLYLNSNNSEDFTSWLKEK
                     NSTQEIYSLIYEDVIDEFNNLKKEYGYVNFDDLLTTMLEVLKDKKFNFKEILVDEYQD
                     TNPL"
BASE COUNT          994 a          315 c          352 g          816 t
ORIGIN      
        1 tatgaactaa ttttatgtaa cttttatagg ctctattttt atgtcaggat ttgggagtat
       61 cccaaataat tccataagat ttaaaataga aagcccaaag tggctataat gtggtcatta
      121 aaagcgaaaa actacactta attctttcac ccactaggtg cagtttttcc acaaggattg
      181 tattatgaca caaacaattt tagattttca tttaacaaca actaatgaaa aattaacacc
      241 taggtctggt gtgataattt taggtgagta tctcaaaggt ttaggtttag aaaaactatg
      301 caattctcat ttaccattac ctttaaatca caaaggatat aatccatttg aatttgttta
      361 tccacttgta ttaatgcttc atagtggtgg tagagtaatt gatgatattc gtgaaataaa
      421 attagattat gcattaaaac aaagtttaaa tattaaaaat attccaactg ccagtgggat
      481 tataaagtgg ttgaagcgaa caggtcttgc aggtgtatat ggtattgaag caatcaataa
      541 aactttgatg gaaagatatt taaaaagaat aacacttcat agcgatgaag atttaatttt
      601 agatattgat gcaactgtaa ttgaagctca taaagttact tctaaatata cctataaaat
      661 ggttccagga tatactccca tcttaggaca tatcaatgga gggtatctta tagcaagtga
      721 atttagagat ggaaatatag cacctgcaga tacaaactta gaatttatca aaaaatgtgt
      781 tcaatatctt ccaaaaaata aaaaactaaa atggcttcga gcagatagtg caacttatca
      841 agcagaagtt tttaactatt gtgatgataa caatattaac tttgcaatag gtggacaatt
      901 agataaatca gttgtaaaac aaattaatca aatatctaat tgggaagaac tttcaaaata
      961 tgaagatata ggagagttta ttcatactat gacccatact aaaaatgctt ttcgtatgat
     1021 tgttattaga agaaatatta cacctatgtt gccacattta gaagatattc tctcagacaa
     1081 agagaaagca caatatcata aagagagatt ttatgtaatg gctacaaata ataatactct
     1141 ttcaaaagaa gaaataatta aactttatcg tcaaagagga gaaacaagtg aaaataaaat
     1201 taaagaattc aaaaatggtt tcaatatgga ctatcttcca tcaagagatt ttatatctaa
     1261 tgctttatac tttcaaatag gtactttagc ctataacctt tttattttat ttaaaactat
     1321 ccttgatgaa aatttacaac accatacggt aaaaacaata cgatataaga tttataatat
     1381 agcagggaaa gttataagtc attcaagaaa aactatttta aaagtaaatg atgaatttat
     1441 agagatactt caaaggataa gatataaagc ccatgaagct tctattgaat aaataaaatt
     1501 ttttgcattg ttttttttaa attgttgata aagaataaag tattccctga aaaaccctat
     1561 aaaatgaaaa tatgataaaa atctacaata tttcatcaca gataaattaa atactcaaat
     1621 aaatttctca aaagatatac agttgcaaaa atattatgaa aaatttcctt tgattatgaa
     1681 ctaattttat gtaactttta taggctctat ttttatgtca ggatttgggt tttcaatcta
     1741 gcttaatcct tttatgataa tattccaaaa tgatttcatt ctatacaaaa atatacataa
     1801 gcctttaaat atgccactat caaatcttaa tcaagaacaa aaagaagcca tcctttgtaa
     1861 aagcggatac aatcttataa ttgcaagtgc aggaactggt aaaacttcaa caattgtagg
     1921 aagaatttct catttactca ataatggagt aaaaccaaat gaaattttgc ttttaacctt
     1981 tacaaataaa gctgcttctg agatggtttc aagagtttct aatatattct caaaacagat
     2041 agcaaaagag attatggctg gaacattcca ctctgtatca tataaacttt taaaagaact
     2101 agagataaat attactttaa aacaaccaaa tgaacttaaa actcttttta aatcaattta
     2161 cgaaaaacga gtttttatgg atagaagtga tgaagcaaac ccatatgatg gtggttattt
     2221 atatgatatg tactctttat acttaaattc aaataatagt gaagatttta ctagctggtt
     2281 aaaagaaaaa aattcaactc aagagatata tagcttaata tatgaagatg ttattgatga
     2341 gtttaataat ttaaaaaaag agtatggata tgtaaatttc gatgatttat taacaacaat
     2401 gcttgaagtt ttgaaagata agaaatttaa tttcaaagag atacttgtag atgaatatca
     2461 agatacaaat cctttac
//
LOCUS       DAOQAN010000125         2470 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_60908_length_2470_cov_2.749482, whole genome shotgun sequence.
ACCESSION   DAOQAN010000125 DAOQAN010000000
VERSION     DAOQAN010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60908_length_2470_cov_2.749482"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(27..1376)
                     /gene="radA"
                     /locus_tag="PKO10_02830"
     CDS             complement(27..1376)
                     /gene="radA"
                     /locus_tag="PKO10_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013135913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HNW66117.1"
                     /translation="MAKKKNTLFECQHCGEQSSKWLGKCPSCDSWDSFLELNEVQQEV
                     LKQTITANSSSSKARPITEILQDDVSRFSSFNYEFDLVLGGGVVPGSLTLIGGSPGVG
                     KSTLLLKVAGSIAKSGKKVLYVSGEESAGQIKLRANRLDANHNELFLLSEIKLEEIMS
                     ELLRIDYEVCIIDSIQTIYSGHLNSSPGSVSQVREITFELMRKAKESNIAMFIIGHIT
                     KDGSIAGPRVLEHMVDTVLYFEGEASRELRMLRGFKNRFGSTSEIGIFEMTQEGLVSA
                     KDIASKFFDKSKSQSGSSLTVSMEGSRAIILEVQALVSETTFPNPKRSATGFDTNRLT
                     MLLALLEKKLDLPFNHYDVFINISGGIKIKESSADLAVIAAIISSFRDRPISKESVFI
                     GEVSLTGEIKDVYSIDLRLKEAQAQGIKKAIIAQKTNLKLDLKTYAVDEVSKVIELF"
     gene            1515..>2470
                     /locus_tag="PKO10_02835"
     CDS             1515..>2470
                     /locus_tag="PKO10_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HNW66118.1"
                     /translation="MKKSITIIDTFGFLFRSYFALPPLRSNNGFPTGLLTGFMNFVAG
                     IGKDFKTDYIVFALDAKGTTFRNELFDNYKAQRPDVPEDLLTQLPVAISWVEKMGFKI
                     AIRTGYEADDMVASIAKDAKEKGFEVRIVSHDKDLYQLIDDENSVYLFDPTKKQIINE
                     AKCIEKYGVTPKQFIDYQALVGDTADNIPGVKGVGAKTAQTLIEQFGTLENIYENIEN
                     IDKKRTKELLIEGKENAFLSKQLVTLKDDCHFISNIDEFSLPLENPILKIASDLESYD
                     MHRIIERVNRNGLNYKTEVPKKAEDEKIEYILLNNENDLTLA"
BASE COUNT          866 a          390 c          350 g          864 t
ORIGIN      
        1 agaattagat tattaggtaa tagagcttaa aaaagctcta ttacttttga tacttcatca
       61 actgcataag tttttaaatc taattttaga ttcgtttttt gagcaattat tgctttttta
      121 attccttgag cttgagcctc ttttaatctt aaatctatag aataaacatc ttttatttcg
      181 ccagttagtg aaacttcacc aataaataca gactcttttg aaattggtct atctcgaaaa
      241 cttgagataa ttgcagcaat tactgctaaa tcagcactac tctctttaat ctttattcca
      301 ccactaatat ttataaacac atcataatga ttaaaaggta agtctaattt tttctctaaa
      361 agagctaaaa gcatagttaa acggtttgta tcaaaacctg tggcacttct ttttggattt
      421 ggaaaagttg tttcgcttac aagtgcttga acttcaagaa taattgcacg gcttccttcc
      481 atactaacag ttaaacttga accactttga cttttgcttt tatcaaaaaa ctttgacgct
      541 atatcttttg cacttacaag tccttcttgt gtcatctcaa aaattccaat ttctgaagtt
      601 gaaccaaacc tatttttgaa acctcttagc attcttagtt ccctgcttgc ttctccttcg
      661 aagtataaaa ctgtatcaac catatgttct aacactctag gacctgcaat acttccatct
      721 tttgtaatat gaccaattat aaacatagca atatttgact cttttgcttt tctcataagc
      781 tcaaaagtaa tctctcgaac ttgtgaaaca cttccagggc ttgagtttaa atgacctgaa
      841 tatattgttt gaattgagtc aataatacaa acttcataat caattctaag aagttcactc
      901 ataatctctt caagttttat ctcacttaat aagaaaagtt cattgtggtt tgcatcaaga
      961 cgatttgctc ttagttttat ctgtccagca ctctcttctc ctgatacata aagtactttt
     1021 ttgcctgact ttgcaatact tcctgcaact tttaaaagaa gagttgattt tccaactcct
     1081 gggcttcctc caataagtgt taaacttcca ggaacaactc ctccacctaa aactaaatca
     1141 aattcataat taaaagatga aaatcttgaa acatcatctt gcaaaatctc tgtgattggt
     1201 cttgcttttg aactagaact atttgctgtt atggtttgct ttaaaacctc ttgttgtact
     1261 tcatttaact ctagaaaact atcccaagag tcgcaactag gacattttcc aagccattta
     1321 ctagattgct caccacaatg ttggcactca aatagagtat tttttttctt tgccatattt
     1381 tgcctttttg gaataaaatt ttgtaagata aattaaaatt aataagatta ttaaatccca
     1441 aattttacaa taaacccatt tttaaaagag tttattatat tataaaatct ttaaactcac
     1501 attgaaagaa tattatgaaa aagagcatta caattatcga tacatttgga tttttattta
     1561 gaagttattt tgcacttcca cccttgcgat caaataatgg tttcccaact ggacttttaa
     1621 caggttttat gaactttgtt gctggaattg gaaaagattt taagacagat tatatagttt
     1681 ttgctcttga tgcaaaaggg acaactttta gaaatgagtt atttgataac tataaagctc
     1741 aaagaccaga tgttcctgaa gatttattaa ctcaacttcc agttgcaata tcttgggttg
     1801 agaaaatggg atttaaaata gctattagaa ctggatatga agctgatgat atggttgctt
     1861 caattgcaaa agatgcaaag gaaaaaggct ttgaggtaag aattgtatct catgataaag
     1921 atttatatca actaattgat gatgaaaata gtgtatattt gtttgaccca actaaaaaac
     1981 agattattaa tgaagcaaaa tgtatagaaa aatatggagt tactccaaag cagtttatag
     2041 attatcaagc attagttggt gatacagctg ataatattcc aggagtaaaa ggagttggtg
     2101 caaaaacagc acaaacttta atagagcaat ttggaacact tgaaaatatc tatgaaaata
     2161 ttgaaaatat tgataaaaaa agaacaaaag agctattaat tgaaggaaaa gagaatgctt
     2221 ttttatcaaa acaacttgta actttaaaag atgattgtca ctttatatca aatatagatg
     2281 agttttcttt gccgcttgaa aatccaattt taaaaatagc ttcagattta gagtcttatg
     2341 atatgcacag aatcattgaa agagtaaata gaaatggttt aaattataaa acagaagttc
     2401 caaaaaaagc agaagatgag aaaattgagt atattttatt aaataatgaa aatgatttaa
     2461 ctttagcaat
//
LOCUS       DAOQAN010000126         2464 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_61130_length_2464_cov_2.630137, whole genome shotgun sequence.
ACCESSION   DAOQAN010000126 DAOQAN010000000
VERSION     DAOQAN010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61130_length_2464_cov_2.630137"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..544
                     /locus_tag="PKO10_02840"
     CDS             23..544
                     /locus_tag="PKO10_02840"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HNW66119.1"
                     /translation="MADILFIALAFFAITGAIAMLVYKSPMFSALGLLITMLSVAGMF
                     ALLNATLLFMVQIIVYAGAIMALILFILMFLNIKEEDLPKEPKKFMLIGFGAILMIPL
                     NILVLKAVSNLPSKDLYPIEGDFGTIKPVGLILYNDWIIAFELISILLLIALVGSVVL
                     AKRRKTKITSKED"
     gene            548..862
                     /gene="nuoK"
                     /locus_tag="PKO10_02845"
     CDS             548..862
                     /gene="nuoK"
                     /locus_tag="PKO10_02845"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013136455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HNW66120.1"
                     /translation="MISLTSYAFVSMILFSIGAIGVIARRNIFVIYMSLELMLNAINL
                     FLVTFARYHFNVDPQIVSIMVISIAAAEAAIFLSIVILLFRSKKSLDTDLFTSLSQGE
                     KR"
     gene            859..>2464
                     /gene="nuoL"
                     /locus_tag="PKO10_02850"
     CDS             859..>2464
                     /gene="nuoL"
                     /locus_tag="PKO10_02850"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HNW66121.1"
                     /translation="MSTNLLVWIILAPLLGAILNGGLYFYNIKKQKISENYFAIIGTL
                     TPLISFLITLSLFLRMIEEKIIFKQHIFTWLNVENLNIDMAFLGDNLSIFMSMFVTFV
                     GWLIHIYAVGYMKDDEGFGKFFAYFNLFLASMLILVLADNPIILFIGWEGVGVCSYLL
                     IKFYYGEKNNVIAANKAFIVNRVGDFGFLLGVATLFFALGQVDLSFSSIEANLSNASN
                     GWLLLAGILLFVGAMGKSAQIPLYTWLPDAMAGPTPISALIHAATMVTAGVYMVARFN
                     FLYTGIEEIGIFIAYIGAFSALLAAIIATKQTDIKKILAYSTMSQLGYMFIAVGLGFY
                     SSGLFHVFTHAFFKAMLFMGAGGVIIALHHEQNIFNIAKHRAILPIISATFLIGVIAI
                     SGIPPFSGFFSKDAILAAAFQEGHYLIYGIALFTAFLTAFYMFRLYFVVFVTPNHHKK
                     EYVYTSKTITFPLVVLAVGAVTAGFLNLPSIFGGTHMVDTWLGSLNSKTITLSHSTEY
                     ILMALSVLVASFGIFIAYKKYARFDLE"
BASE COUNT          783 a          316 c          399 g          966 t
ORIGIN      
        1 agagatcaaa ggattttaaa taatggctga tatactattt attgctttag cattttttgc
       61 aattactggt gctattgcta tgctagttta caaaagtcca atgtttagtg cattggggct
      121 tttaattact atgctaagtg ttgctggaat gtttgcactt ttaaatgcaa ctttgttgtt
      181 tatggtacaa attatcgttt atgctggtgc tattatggct ttaattctat ttattttaat
      241 gtttttaaat attaaagaag aggatttacc aaaagaacct aaaaagttta tgcttattgg
      301 ttttggtgct attttgatga ttcctctaaa tattttagtt ttaaaagctg tatcaaattt
      361 acctagtaaa gatttgtatc ctatagaagg tgattttgga actataaaac cagttgggct
      421 tattctttat aatgattgga ttatagcatt tgaattaata tctattttac ttctaattgc
      481 acttgtaggt tctgttgttc ttgcaaaaag aagaaaaact aaaataactt caaaggagga
      541 ttaaaaaatg atatcattaa cttcttatgc ttttgtatct atgattttat tctcaattgg
      601 tgcaattgga gtaattgcta gaagaaatat ttttgtgatt tatatgtcac ttgaacttat
      661 gttaaatgct ataaatctat ttttagtaac atttgctaga taccatttta atgttgatcc
      721 tcaaattgtt tcaattatgg ttatatcaat tgcagcagct gaagctgcaa tatttctatc
      781 aatagtaata ttgctgttta gatctaaaaa atctttggat acagatctat ttacatctct
      841 atctcaagga gaaaaaagat gagtacaaat cttttagttt ggataatatt agctccttta
      901 cttggggcta ttttaaatgg tggtttatat ttttataata tcaaaaaaca aaaaatatca
      961 gaaaattatt ttgcaataat tggaacatta actcctttaa tctctttttt aataactctt
     1021 agtctatttt taagaatgat tgaagaaaaa attatattta aacaacatat atttacatgg
     1081 ttaaatgttg aaaacttaaa tatagatatg gcatttttgg gtgataattt atctattttt
     1141 atgtcaatgt ttgtaacttt tgttggttgg ttaattcaca tctatgctgt tggatatatg
     1201 aaagatgatg aaggttttgg taaatttttt gcttacttta atctattcct agcatcaatg
     1261 ttgattcttg tacttgcaga caatccaata attttattta taggttggga aggtgttgga
     1321 gtttgttcat accttttaat caaattctat tatggtgaaa aaaataatgt tattgctgca
     1381 aataaagcat ttattgtaaa cagagttgga gattttgggt tccttttagg agttgcaaca
     1441 ctattttttg cattaggtca agttgatcta tctttctctt caattgaagc aaatttatca
     1501 aatgcatcaa atggttggct tttattagct ggtatattat tatttgtagg agctatggga
     1561 aaatcagctc agattccact atatacatgg cttccagatg caatggcagg tccaacacca
     1621 atttctgcac taattcacgc agcaacaatg gttacagctg gggtttatat ggttgcgaga
     1681 tttaatttct tatatacagg aatagaagaa attggaatat tcatagcata tattggagca
     1741 ttttcagctc ttcttgctgc tattattgca acaaaacaga cagatattaa aaaaatactt
     1801 gcatactcaa ctatgagtca attaggttat atgtttatag ctgttggact tggattttac
     1861 tcaagcggat tatttcatgt tttcacacat gcattcttta aggcaatgct atttatggga
     1921 gctggtggag ttataattgc acttcatcat gaacaaaata tttttaatat tgctaaacat
     1981 agagcaatat tgccaataat ttctgcaaca ttcttaatag gagttattgc aatttcagga
     2041 attccaccat tttctgggtt cttctctaaa gatgcaattt tagcagcagc atttcaagaa
     2101 ggtcactact taatttatgg tatagcacta tttactgcat ttttaactgc attttatatg
     2161 tttagactat attttgtagt ttttgttaca ccaaatcatc ataaaaaaga gtatgtatat
     2221 acaagtaaaa ctatcacttt cccacttgta gtattggcag taggagctgt tacagcagga
     2281 tttttaaatc taccttctat atttggtgga acacatatgg tagatacttg gcttggaagc
     2341 ttaaattcaa aaacaattac tctatctcat agtacagagt atattttaat ggctctttca
     2401 gttctagttg cttcttttgg tatatttatt gcatataaaa aatatgcaag atttgattta
     2461 gaaa
//
LOCUS       DAOQAN010000127         2461 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_61255_length_2461_cov_2.433500, whole genome shotgun sequence.
ACCESSION   DAOQAN010000127 DAOQAN010000000
VERSION     DAOQAN010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61255_length_2461_cov_2.433500"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..761)
                     /locus_tag="PKO10_02855"
     CDS             complement(63..761)
                     /locus_tag="PKO10_02855"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNW66122.1"
                     /translation="MGLDLYARIEPYLDFEEEIYSLHKEFLKFIMVNDLDNIIDIGCG
                     QGYFLNNLAINKKKAFGIDLSAEQIKICEEKGLNAKAIALEDVEEKFDCATAIFDVLN
                     YIPKIELEKFILQVNKVLNPNAYFIFDVNTYFGFDEVAQGTINIDIEDKFIAIDAFFE
                     NDKLQTNITLFEKEKSGKYRKEQDKIVQEYHKKEYLEKILKKSGFEIVEIKEFFLHTD
                     ESADKLIFICKKYL"
     gene            complement(761..1369)
                     /gene="hisG"
                     /locus_tag="PKO10_02860"
     CDS             complement(761..1369)
                     /gene="hisG"
                     /locus_tag="PKO10_02860"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HNW66123.1"
                     /translation="MLTIALPKGRIADETLDRFEKAFGEKFVFEDRKLILEKSGFKFL
                     NVRNQDVPTYVMHGAADLGVVGLDVLEEKEYDLIKLLDLKLGRCKVAFGLRAGEKLNF
                     DKSKITIATKHEKIAKKYFEQKAMAVEIIKLYGSIELAPLVGLCDCIVDIVETGETMK
                     QNGLEVGPTIMETTAHLIANKNSFYAKKDLIFDLKDKLEKFL"
     gene            complement(1347..1982)
                     /locus_tag="PKO10_02865"
     CDS             complement(1347..1982)
                     /locus_tag="PKO10_02865"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014473179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HNW66124.1"
                     /translation="MILCDIGNTSTKFFIDKNIKNYYKNDKIPKFKDDIFYISVNEKA
                     TKKLLKKNPHAKNLASFIDFRTSYIGLGVDRAVACYFEENAVVIDAGSAITVDIIEDK
                     KHIGGFIFLGLNSFFKAYKNISKKLEISKSDFEKNINLDKIPLQTKDAVFFALLKSII
                     LPIKEICKNKNIIFTGGDGEFLSQFFENSIYKKDLIFENMKRIIDANNSPS"
     gene            complement(1997..2209)
                     /locus_tag="PKO10_02870"
     CDS             complement(1997..2209)
                     /locus_tag="PKO10_02870"
                     /inference="COORDINATES: protein motif:HMM:NF014505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HNW66125.1"
                     /translation="MEIKTFDIVLCEFYFSNLNQSKKRPVLVFKDNLPFDDFIAIPIS
                     SKIGNMTNDEILIELKNFVNILSIEF"
     gene            complement(2200..2415)
                     /locus_tag="PKO10_02875"
     CDS             complement(2200..2415)
                     /locus_tag="PKO10_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66126.1"
                     /translation="MHTVKLKINDDIYNHIMFFLKSLDNNKIKILEEKKQKTVSKDDN
                     EDTKLFSNHSANLIEDWQDLEEDNIWK"
BASE COUNT          812 a          381 c          225 g         1043 t
ORIGIN      
        1 ggggtttgtt gagtgtaaaa tttgattttt actctttttt gagaaagtca agagtctaag
       61 tattataaat attttttaca aataaatatt agtttatctg cactttcatc tgtatgaaga
      121 aaaaactctt ttatctcaac aatctcaaaa ccagattttt ttagaatttt ttctaaatac
      181 tcttttttat ggtactcttg tactatttta tcttgttctt ttctatattt cccacttttt
      241 tctttttcaa aaagtgttat atttgtttga agtttatcat tttcaaaaaa agcatctatt
      301 gctataaatt tatcttcaat atcaatattt atagttcctt gagctacttc atcaaaacca
      361 aaatatgtgt ttacatcaaa tataaaataa gcatttggat ttaaaacttt attaacttgt
      421 aaaataaact tttctaactc aatttttgga atatagttta aaacatcaaa aattgcagtt
      481 gcacaatcaa atttttcttc aacatcttca agagctatag cttttgcatt taagcctttt
      541 tcttcacaaa tttttatttg ttcagcactt aaatctatcc caaaagcttt ttttttgttt
      601 atcgctaagt tatttaaaaa atatccttgt ccacaaccaa tatcgattat attgtctaaa
      661 tcatttacca taataaattt caagaactct ttatgaagag agtaaatctc ctcttcaaaa
      721 tctaaatatg gctctattct tgcatataaa tcaagtccca ttataaaaat ttctctagtt
      781 tatcttttaa atcaaagatt aaatcttttt ttgcataaaa tgagttttta tttgctatta
      841 agtgagctgt tgtctccatt atagttggtc caacttctaa accattttgt ttcatagttt
      901 caccagtttc aacaatatca acaatacaat cacatagccc aaccaaaggt gctagctcaa
      961 ttgagccata aagttttatg atttctactg ccatagcttt ttgttcaaaa tattttttag
     1021 ctattttttc atgttttgta gctattgtta ttttactttt atcaaaattt agcttctctc
     1081 cagctcttaa accaaaagca actttacatc gtccaagttt taaatccaaa agcttaatta
     1141 agtcgtactc tttttcttct aaaacatcaa gtccaacaac tcctaaatca gcagctccat
     1201 gcataacata agttggtaca tcttgatttc ttacatttaa aaacttaaaa ccactttttt
     1261 ctaatattag ttttctatct tcaaaaacaa acttctctcc aaaagctttc tcaaatctat
     1321 caagagtttc atctgcaatt cttcctttag gaagggctat tgttagcatc tattatcctt
     1381 ttcatatttt caaatattaa atctttttta tatatactat tttcaaaaaa ttggctcaaa
     1441 aactctccat ctccacctgt aaatattata tttttatttt tacaaatctc ttttataggt
     1501 aaaatgatag attttaataa agcaaaaaaa acagcatctt ttgtttgaag cggtatttta
     1561 tccaaattaa tatttttttc aaaatcactt tttgatattt ctagcttttt tgagatattt
     1621 ttataagctt tgaaaaaact atttaatcct aaaaatataa aaccaccaat atgtttttta
     1681 tcttcaataa tatctacagt tattgcactt cctgcatcaa taacaactgc attttcttca
     1741 aaataacaag caactgctct atctactcct aaacctatat aagaagttct gaaatctata
     1801 aaactagcta aattttttgc atgaggattt tttttaagga gtttttttgt agctttttca
     1861 tttacactta tgtaaaaaat atcatcttta aactttggga ttttatcatt tttgtagtaa
     1921 ttttttatat ttttatctat aaaaaatttt gtacttgtat ttccaatatc acataaaatc
     1981 aaattttgcc tttgttttaa aattcaatac tcaaaatatt tacaaaattt tttaactcta
     2041 ttaatatttc atcatttgtc atatttccta ttttactact aattggaatt gcaataaaat
     2101 catcaaaagg taaattgtct ttaaatacaa gtactggtct tttttttgat tgatttaaat
     2161 tgctaaaata gaactcacaa agtacaatat caaaagtttt tatttccata tattatcttc
     2221 ttctaagtct tgccaatctt ctataagatt tgcagaatgg tttgaaaata gttttgtatc
     2281 ttcattgtca tcttttgata ctgttttttg ttttttctct tctaaaattt ttattttatt
     2341 attgtcaaga ctttttagga aaaacattat atggttataa atatcatcat ttatttttag
     2401 tttaactgta tgcatatatt ttcctttata aattttttaa acgattatat caaattagga
     2461 a
//
LOCUS       DAOQAN010000128         2452 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_61615_length_2452_cov_2.239049, whole genome shotgun sequence.
ACCESSION   DAOQAN010000128 DAOQAN010000000
VERSION     DAOQAN010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61615_length_2452_cov_2.239049"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..1594
                     /locus_tag="PKO10_02880"
     CDS             98..1594
                     /locus_tag="PKO10_02880"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014473224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNW66127.1"
                     /translation="MIYERPKYKNQYENFIGGEWIAPSSGEYIENISPVDGKLLTKIP
                     RSNEKDVDLAVEAAKKGFEEFKHTSVTQRSALLNKIADIVEANLETLAVAETLDNGKA
                     VRETLNADLPLFIDHFRYFASVIRAESGTISDLDENTISQEIYEPYGVVAQIIPWNFP
                     LLMAAWKLAPAIAAGNCVVLKPASSTPLSILLFLDLIKDVLPKGVINVVNGAGGKIGK
                     HLVTHRDVKKVAFTGETSTGQEIMRYATENIIPSTLELGGKSPNVFFKSIMDADDEFF
                     DKAIEGLVLFAFNSGEVCTCPSRALIEESIYEPFMKRVIERVKAIKLGNPLDTENTMG
                     AQNSFNQKEKIAKYLKIAKEDGAECLVGGDIYHSSINPDGFYIEPTIYKGHNKMRIFQ
                     EEIFGPVLSVTTFKDEKEAIEIANDTIYGLGAGVWSRDAHQIHRVSRAIQAGRIWVNC
                     YHVYPAHASFGGYKKSGIGRETHMMMLNNYRHTKNILTSFNTKALGFF"
     gene            complement(1625..2377)
                     /locus_tag="PKO10_02885"
     CDS             complement(1625..2377)
                     /locus_tag="PKO10_02885"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HNW66128.1"
                     /translation="MKKLKNLNPEDFEQECTTIWSFKRRGNWATHKSKYRGNWAPEVV
                     RNLLLRYSNENDYVLDPMIGGGTTAIECKLLNRNLLALDINPNAIELTKEALNFDCEY
                     NPKIRVELNDSRKISILKDESIDFILNHPPYADIIKYSEKQIDEDLSNIHDLEKFCDE
                     YEKVIKELYRVLKPNKYCAILIGDTRRNKMYQPLAFMVMQRFLQNGFTLKEDIIKHQH
                     NCKATGFWVNKSKEANFLLIMHEHLFVFQKVK"
BASE COUNT          835 a          338 c          399 g          880 t
ORIGIN      
        1 cttgcaaaat agcctaaaac tcttttttat aaatgcaact ataaagctac ttttgtgtga
       61 tattatcctt gatatctaat atcaaaaagg agttattatg atttacgaaa gaccaaagta
      121 taaaaatcag tatgagaatt ttataggtgg agagtggatt gctccaagta gtggagagta
      181 tattgaaaat atctctccag ttgatggaaa attacttaca aaaattccaa gatcaaatga
      241 aaaagatgtt gatttagcag ttgaggctgc aaaaaaaggt tttgaagagt ttaagcatac
      301 atctgtaact caaagaagtg ctttattaaa caaaattgca gatatagtag aggcaaattt
      361 agagactttg gcagttgctg aaacacttga taatggaaaa gctgtaagag agactttaaa
      421 tgcagattta cctctgttta ttgaccattt taggtatttt gcatctgtta taagagctga
      481 atctgggaca atttctgatt tggatgagaa cactatatct caagagattt atgagcctta
      541 tggagttgtt gctcaaatta ttccttggaa tttcccactt ctaatggcag cttggaaact
      601 agcacctgca attgctgctg gaaattgtgt tgttttaaaa ccagctagtt caactccact
      661 ttcaatactt ctatttttgg atttaatcaa agatgtttta ccaaaaggtg ttataaatgt
      721 tgtaaatgga gcaggtggaa aaataggtaa acatcttgta actcatagag atgttaaaaa
      781 agttgcattt actggagaga caagtacagg tcaagagatt atgagatatg caactgaaaa
      841 tataattcca tcaactctag agcttggtgg aaaatctcca aatgtattct ttaaatctat
      901 aatggatgct gatgatgagt tctttgataa ggcaattgaa gggcttgtac tatttgcatt
      961 taatagtgga gaggtttgta cttgtccatc aagagcttta attgaagagt caatttatga
     1021 accatttatg aaaagagtta tagaaagggt aaaagctata aaacttggaa atcctcttga
     1081 tacagaaaat acaatgggag ctcaaaactc ttttaaccaa aaagagaaaa ttgcaaaata
     1141 tctaaaaatt gcaaaagagg atggggctga gtgtttagtt ggtggagata tttatcactc
     1201 ttctataaat ccagatggat tttatataga gccaacgatt tataaaggtc ataataaaat
     1261 gagaattttc caagaggaga tttttggacc tgttttatct gtaactacat ttaaagatga
     1321 gaaagaggct attgaaattg caaatgatac tatttatgga ttgggtgctg gagtttggtc
     1381 aagagatgca caccaaattc atagagtatc aagagctatt caagctggaa gaatttgggt
     1441 aaattgttat catgtttatc cagctcatgc ctcttttggt ggatataaga aatctggaat
     1501 tggaagagag actcatatga tgatgttaaa taactataga catacaaaaa atattttaac
     1561 ttcatttaat acaaaagctt taggattttt ttaatctctt aagataatag aggcttttta
     1621 gcctctattt tactttttga aatacaaata gatgctcatg cataatcaat aaaaagtttg
     1681 cttctttgct tttatttacc caaaatccag tagctttaca gttgtgttga tgttttataa
     1741 tatcttcttt tagtgtaaat ccattttgca aaaatctttg cattaccata aaagctaaag
     1801 gttgatacat cttatttctt cttgtatctc ctattaaaat tgcgcaatat ttatttggtt
     1861 ttaaaactct ataaagttct ttgataactt tttcatattc atcacaaaat ttttctaaat
     1921 catgaatatt tgataaatct tcatcaattt gtttctcact atacttaata atatctgcat
     1981 aaggtggatg atttaagata aaatctatac tttcatcttt taaaatagat atttttcttg
     2041 aatcatttag ttcaactctt attttaggat tatattcaca atcaaaattt aaagcctctt
     2101 ttgtaagctc tattgcattt ggatttatgt caagagctaa taaatttcta tttagaagtt
     2161 tacactcaat tgctgttgtt cctccaccaa tcattggatc aagcacataa tcattttcat
     2221 ttgaatatct taaaagtaaa tttcttacaa cttcaggagc ccagtttcct ctatattttg
     2281 atttatgagt tgcccaattt cctcttcttt taaaactcca aattgttgta cactcttgtt
     2341 caaaatcttc tgggtttaag ttctttagct ttttcatctt tgcactaata aaaaatattt
     2401 ttcaacattg atttcaaaaa tatgttctct tgaaaaatca attattttat tt
//
LOCUS       DAOQAN010000129         2429 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_62515_length_2429_cov_2.780539, whole genome shotgun sequence.
ACCESSION   DAOQAN010000129 DAOQAN010000000
VERSION     DAOQAN010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62515_length_2429_cov_2.780539"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..558)
                     /gene="purE"
                     /locus_tag="PKO10_02890"
     CDS             complement(64..558)
                     /gene="purE"
                     /locus_tag="PKO10_02890"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014475119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HNW66129.1"
                     /translation="MKFVSIIMGSKSDYEIMKNCADTFEQFKVKYEIIISSAHRSPER
                     TKEYVKEAEEKGAIAFIAAAGMAAHLAGALAATTTKPIIGVPMKGGAMDGMDAMLSTV
                     QMPAGMPVATVALGKAGAINAAYLAMQILAISDKDLAIKLKEDRIVKAKAVESDSKDI
                     EVIL"
     gene            complement(597..1853)
                     /locus_tag="PKO10_02895"
     CDS             complement(597..1853)
                     /locus_tag="PKO10_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014469298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66130.1"
                     /translation="MIFFRATNSINIKITDGTILKYNRFKAPIGIENETIYLTTYDDF
                     NDLKDIFAKVDKSKYNAKIINEQSVLKNPSFPTQLGVIIGNESIERLELKDLRFLELK
                     EQNFLNQLKSFHKSSIKVAIIGSLGSSISQMISGMAALRIFYNKLKEIYKVVKLDVYI
                     KASNNSYYNRDKSIYLTQDYINEILPLAINSKAICEYDYFVDNSIDITKILDINPVDA
                     WLFKFGIDYKKISDLEKFSQLKTDHFELTKGLKQKIQESKQRGKLLLFHPYSANINKS
                     IPQNFAIEILKNLIEKLDDYTIVSTLLIDSKIKADNFLDLSLYSKTIEDFIYIVASCD
                     KLITAYTSALHIADCFMIPTVCIATFKEYEEQLKYYKYTKTIFVKDSSKNLSKFIYEN
                     DSLTINKFEEWKKLKIEDIVKVLESF"
     gene            complement(1855..>2429)
                     /locus_tag="PKO10_02900"
     CDS             complement(1855..>2429)
                     /locus_tag="PKO10_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HNW66131.1"
                     /translation="HIKEILDSKAVDSLKIEGRTKSPYYAAVTAKAYREAIDDYYENK
                     FDASKYQKELHTTKNRGFTDAYLIHKPFEKLDSQNHDYALSKGSFEVTGLVSEDEEHF
                     YCKYKVYPNEDIEIFTPHNTVLHECDNEIGRVFKKENGLYYINFKKILTQENKELESV
                     HSGNVNKIKLPCKLPYLTMFRVENISENIE"
BASE COUNT          779 a          395 c          253 g         1002 t
ORIGIN      
        1 gtagataata gcctctttgc accatttaca gtttggtaaa ataaacagtg caataggctt
       61 caattataaa ataacttcaa tatctttaga atcactctca acagcttttg cttttacaat
      121 tctatcttct tttaatttta ttgctaaatc tttatcgcta attgctaaaa tttgcattgc
      181 taaataagca gcattaattg cacctgcttt tcctaaagca acagttgcaa caggcatacc
      241 agctggcatt tgaacagttg aaagcatagc atccatacca tccatagcac cacctttcat
      301 aggaacccca ataataggtt ttgttgtagt tgctgctaat gcaccagcca aatgagccgc
      361 cattccagct gctgcaataa atgcaatagc acctttctct tcagcctctt ttacatactc
      421 ttttgttctt tctggacttc tatgagcaga agatataatt atctcatact ttactttgaa
      481 ttgctcaaaa gtatctgcac agtttttcat aatttcataa tctgacttac tacccataat
      541 tattgataca aatttcattt ttgttcctat ttatttaatt ttgaactatt attatatcaa
      601 aaactttcta agacttttac aatatcttct atcttgagtt ttttccactc ttcaaatttg
      661 tttatagtca aactatcatt ctcatatata aattttgata gattttttga gctatctttt
      721 acaaaaattg tttttgtata tttgtaatat tttagttgct cttcatactc tttaaaagtt
      781 gctatacaaa ccgttggtat cataaaacaa tctgctatat gaagtgctga tgtataagct
      841 gtaattagct tatcacaaga tgcaactata tatataaaat cttcaatagt ttttgaatat
      901 aatgataaat ctaaaaaatt atcagctttt attttactat caattaaaag agttgaaaca
      961 attgtataat catctagttt ctctatcaaa ttttttaaaa tctcaatagc aaaattttga
     1021 ggaatagatt tatttatatt tgcactataa ggatgaaaaa gtagaagttt tcctctttgt
     1081 ttactctctt gtatcttttg ttttaatcct tttgttagtt caaaatggtc tgtttttagt
     1141 tgtgaaaatt tttctaaatc tgatattttt ttataatcta ttccaaattt aaaaagccaa
     1201 gcatcaactg gatttatatc taaaattttt gtaatatcaa tactgttatc aacaaaatag
     1261 tcatattcac aaatagcttt gctatttatt gccaatggta aaatctcatt tatataatct
     1321 tgagttaaat atatactttt atctctgttg taataactat tatttgaagc ttttatatat
     1381 acatcaagtt ttacaacttt atatatctct tttaatttat tataaaaaat tcttagtgct
     1441 gccattccac ttatcatttg gctaatacta cttcccaaac ttccaataat agcaactttt
     1501 atactacttt tatggaaact ttttagttga tttaagaaat tttgctcttt taattctaaa
     1561 aatcttaaat ctttaagctc taatctctct atactttcat ttcctataat tacacccaat
     1621 tgagtaggaa aagatggatt ttttaaaaca ctttgctcat ttataatttt tgcattatat
     1681 ttgcttttat caacttttgc aaaaatatct tttaaatcat taaaatcatc ataagttgta
     1741 agatatattg tctcattttc tattccaata ggagctttaa acctattgta ctttaaaatt
     1801 gttccatcag ttattttaat attaatacta tttgtcgctc taaaaaaaat cattttattc
     1861 tatattttca ctaatatttt ctactctaaa cattgttaaa taaggtaatt tacatggaag
     1921 ttttatttta tttacatttc cactatgaac actttcaagc tctttattct cttgcgttaa
     1981 aatcttttta aaatttatat aatatagtcc attctctttt ttaaaaactc ttccaatctc
     2041 attgtcacac tcatgtaaaa ctgtgttatg tggagtgaaa atttcaatat cttcatttgg
     2101 ataaacttta tacttgcaat aaaaatgctc ttcatcttca gatactaaac ctgttacttc
     2161 aaatgagcct ttacttaaag cataatcgtg attttgagaa tctagttttt caaatggctt
     2221 atgaattaaa taagcatctg taaaacccct atttttagtt gtatgcaact ctttttgata
     2281 ttttgaagca tcaaatttat tttcataata gtcatcaata gcttctctat aagcttttgc
     2341 agttactgct gcatagtatg gagattttgt tcttccttca attttcaaac tatcaactgc
     2401 tttgctatca agtatctctt taatatgag
//
LOCUS       DAOQAN010000130         2428 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_62550_length_2428_cov_2.958281, whole genome shotgun sequence.
ACCESSION   DAOQAN010000130 DAOQAN010000000
VERSION     DAOQAN010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62550_length_2428_cov_2.958281"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            78..521
                     /locus_tag="PKO10_02905"
     CDS             78..521
                     /locus_tag="PKO10_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HNW66132.1"
                     /translation="MNQIDNYLRKDKSNSFLVKNKTTLAFDIKVDGKVLSVVVKEYQK
                     CPITSDLLHVDLMVAQKGVRASYFIPVVATGSAKGLKNKGLLMLHTRRIPVKCTIENL
                     PNNITLDVTNLDTGDNILLRDITLPENVDCYLDPRVPIVGVIKAK"
     gene            531..1094
                     /gene="pth"
                     /locus_tag="PKO10_02910"
     CDS             531..1094
                     /gene="pth"
                     /locus_tag="PKO10_02910"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012856016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNW66133.1"
                     /translation="MYLIVGLGNIGEKYQNTRHNIGFLVVDFMTKNLNTTSINNSNFQ
                     STLLKAGYNLFSKPTTYMNNSGIAVKNIMDYYKVDLENIIIIHDDIDLPFGTVKFKIG
                     GGHGGHNGLKSLDSHITKEYIRVRVGIGKPKDKEDVANYVLSDFSKAEFEELNNKIIP
                     HVASCVEALKELSIDEVKAKFTLKQSI"
     gene            1091..2158
                     /locus_tag="PKO10_02915"
     CDS             1091..2158
                     /locus_tag="PKO10_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNW66134.1"
                     /translation="MSILTKYIIEKYLKNFIIILLSLEIFFVGIDFLQNFKSIPQSAN
                     LQLLYIFYNAFFTLTLTLPLSIVFAWISTLITFIKNNEYVAFYSLGAGRRDIFLPILS
                     LGLIFLVVLIFLQMTPLAYSYEQKKKILDNEYFSSTKSDIFLKYNDNFVYFQKLFPLE
                     KRAENIHIFKVDGKDVVETIVAQKAYFQNNKWYIVDAKITTKPKELDMNSKLTIKYEK
                     FLNTLDGFQPKILDNVYEARNDSSLLDAINAMFLLEKQGVNTSKIRGNIYNQLFVPFF
                     IIPLLFLVFAYSSLNSRFFRLGIFVSFGIFGTLIVWGVFFFLFKITSSGVLNPELSLL
                     LPLIIWFIVAIYVYQSRIKTI"
     gene            2170..>2428
                     /locus_tag="PKO10_02920"
     CDS             2170..>2428
                     /locus_tag="PKO10_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNW66135.1"
                     /translation="MSHTKTYGIVPYIVTKKGVKILLCLDVASNNKWGCLKGTKVKNE
                     TAFMCAKREFFEESSISIDIELFEEYFEQINQEKDIGVWLVN"
BASE COUNT          908 a          261 c          362 g          897 t
ORIGIN      
        1 aatcgaggac atatccttat cctttattca acaaagtact atttaattaa attcagaagg
       61 aataaattat actctttatg aatcaaatag acaactattt aagaaaggat aaatcgaata
      121 gttttttagt aaaaaataaa actactcttg cttttgatat taaagtagat ggaaaagttt
      181 taagtgtagt tgtaaaagag taccaaaaat gccctattac ttctgattta ttgcatgttg
      241 atttaatggt tgctcaaaaa ggtgttagag cttcatattt tatcccagtt gtagcaacag
      301 gaagtgcaaa aggtcttaaa aataaaggtc ttttaatgtt acatactaga agaattccag
      361 ttaaatgtac aattgaaaat ttaccaaata acatcacttt agatgttaca aatttggata
      421 caggagataa tattctttta agagatatta cacttccaga aaatgttgat tgttatttag
      481 acccacgagt tccaatcgtt ggtgtaatta aagctaaata attaaaacct atgtatttaa
      541 ttgttggtct tggaaatatt ggtgaaaaat accaaaatac aagacacaac attggatttt
      601 tagtcgtaga ttttatgact aaaaatctta atactacttc tataaataac tcaaactttc
      661 aatcaactct tttaaaagca ggatataatc tattttctaa accaactaca tatatgaata
      721 attcaggaat tgctgtaaaa aatattatgg attactataa agttgattta gaaaatatta
      781 ttattattca tgatgatata gatttacctt ttggaactgt gaaattcaaa ataggtggag
      841 gtcatggtgg gcataatggt ttgaaatctc ttgatagcca tattacaaaa gagtatataa
      901 gagttagagt tggaattgga aaaccaaaag ataaagaaga tgttgcaaac tatgttttaa
      961 gtgattttag taaagcagag tttgaagagt taaataacaa aatcattcca catgttgctt
     1021 cttgtgttga agctttaaaa gagcttagta ttgatgaagt taaggctaaa tttacattaa
     1081 agcagtctat atgagtattt taacaaaata tataattgaa aagtatctta aaaattttat
     1141 tataatttta ctatctttag agatattttt tgtgggaatt gactttttac aaaattttaa
     1201 atctatacct caatcagcaa acttacagtt gttatatatc ttttataatg cattttttac
     1261 actaacttta actcttcctc tttcaattgt ttttgcttgg atttcaactt taataacatt
     1321 tattaaaaat aatgagtatg ttgcatttta ctctttagga gctggaagaa gagatatatt
     1381 tttacctata ttatctttag gtttaatatt tttagttgtt ttaatatttt tgcaaatgac
     1441 acctttggca tactcttatg aacaaaaaaa gaagatttta gataatgagt acttctcaag
     1501 tacaaaaagt gatatatttt tgaaatataa tgataatttt gtctattttc aaaagctttt
     1561 tcctcttgaa aaaagagcag aaaatataca tatttttaaa gtagatggaa aagatgtagt
     1621 tgaaacaatt gttgcacaaa aagcatattt tcaaaataat aaatggtata ttgtagatgc
     1681 aaagattact acaaaaccaa aagagcttga tatgaactct aaacttacaa taaagtatga
     1741 aaaattttta aatacattag atggatttca accaaagata ttggataatg tatatgaagc
     1801 aagaaatgat tcttcattgt tagatgctat aaatgctatg tttttattag aaaaacaagg
     1861 tgtaaacact tcaaaaataa gaggaaatat atataatcag ctatttgttc cattttttat
     1921 aattccactt ctttttttag tatttgcata tagttctttg aatagtagat tttttagatt
     1981 aggaattttt gtatcttttg gtatttttgg aactttaatt gtttggggag tttttttctt
     2041 tttatttaaa attacaagct ctggtgtttt aaatccagag ctatctttat tattaccttt
     2101 aattatttgg tttatagtag ccatctatgt ttatcaatct agaataaaaa ctatatagga
     2161 tttaaaataa tgagtcatac aaaaacttat ggaatagttc catatattgt aacaaaaaaa
     2221 ggtgttaaaa ttcttttgtg tttagatgtt gcaagcaata ataaatgggg atgtctaaaa
     2281 ggtacaaaag taaaaaatga gactgctttt atgtgtgcaa aaagagagtt ttttgaagag
     2341 agttcaatct ctatagatat tgaacttttt gaagagtatt ttgagcagat aaatcaagaa
     2401 aaagatattg gagtttggct tgtaaact
//
LOCUS       DAOQAN010000131         2405 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_63461_length_2405_cov_3.321702, whole genome shotgun sequence.
ACCESSION   DAOQAN010000131 DAOQAN010000000
VERSION     DAOQAN010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63461_length_2405_cov_3.321702"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="PKO10_02925"
     CDS             complement(<1..208)
                     /locus_tag="PKO10_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HNW66136.1"
                     /translation="MQRLVTTSEASEILGISLQGIHYRIRRNQLKSEKKDGKLFVYID
                     EETIKNSLKNDKQNSQENIFERVIQ"
     gene            complement(222..599)
                     /gene="queF"
                     /locus_tag="PKO10_02930"
     CDS             complement(222..599)
                     /gene="queF"
                     /locus_tag="PKO10_02930"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011371640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HNW66137.1"
                     /translation="MKYGEKEILEFDINNEENFWPNEHDKNYIIDIELPEFMAKCPRS
                     GYPDFATIKIQYTPNKRVIELKALKIYINSFMNRYISHENSANEIFDTLFSKLEPKWL
                     KVVADFKPRGNVHTVIEIDSSKI"
     gene            715..1689
                     /locus_tag="PKO10_02935"
     CDS             715..1689
                     /locus_tag="PKO10_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012011926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNW66138.1"
                     /translation="MSSKIFDTVIVGAGVSGLSCAIELKIENIENILLIEKSDNFFNT
                     IRTFYKDGKRVDKYWRNQTINLIGNIPFDGGLKEDVLNYFESLLKKYNIPTIYNNEVE
                     KVIKLDNGIFEVHTKLDIYKAKNVVIAIGKMGKPNKPNYKIASEIQKNINFNLDSCSK
                     NEKILVVGGGNSAAEYAYSLALDDNIVTLAYRKESFTRLNKINENTLKEFEANKKLTI
                     KLNCDINSLEDDNGLVKVNFSNAESENFNRVIYAIGGTAPTDFLKNCGVKFSGNLKPI
                     FDENFECCCKNLYIAGDVNSSSEGSISSSLNHGFLISKAIAKGLKPLA"
     gene            complement(1686..>2405)
                     /locus_tag="PKO10_02940"
     CDS             complement(1686..>2405)
                     /locus_tag="PKO10_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014472719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HNW66139.1"
                     /translation="KKGKNEIYLKDNNALSAYLIENGLENFEFEGMGYNDLLDLFKQV
                     ARYRAMLEQLAKRYSLIEVLKYLIENSDLVNLEFPTLYEKVKLFLEARGYNILSKTIT
                     ETKIQLFVQTNAGLEELIIDEELFASPYFSESTFIFTKLKERDLTLFEGRDLIELLEE
                     IESLAKKGAYIQRYKGLGEMNPEQLWETTMTPENRRLLRVKIEDAEAASDTFTLFMGD
                     EVEPRRNYIESHAKDVEHLDV"
BASE COUNT          848 a          317 c          314 g          926 t
ORIGIN      
        1 attgaattac tctttcaaaa atattttctt gactattttg tttatcattt tttaaactat
       61 tttttatagt ttcttcatct atataaacaa atagtttgcc atcttttttc tctgatttta
      121 gttgatttct tctaatacgg tagtgaatac cctgcaaaga gattcctaaa atctctgaag
      181 cttctgaagt tgttaccaat ctttgcaagg ttactctctt tttatatttt tgagctatct
      241 atttcaataa ctgtatgaac atttcctctt ggtttaaaat cagcaactac tttaagccat
      301 tttggttcta gttttgaaaa aagtgtatca aaaatctcat ttgcagagtt ttcatgagag
      361 atatatctat tcataaatga gtttatataa attttaagtg ctttaagctc aataactctt
      421 ttatttggtg tatattgaat cttaatagtt gcaaaatcag ggtaaccact tcttggacat
      481 tttgccataa attctggtaa ctcaatatca attatatagt ttttatcatg ttcgtttggc
      541 caaaagtttt cttcgttatt tatatcaaac tctaaaatct ctttttcacc atattttatt
      601 tccattagta accttttttt aaaaattttt gaaaatattc tatccaaata tctcttataa
      661 agctaattcc caaaaataag gataaaattt aaatatattt ttaaaggtta aaaaatgagt
      721 tcaaaaatat ttgatacagt aattgttgga gctggtgttt caggactgag ttgtgctatt
      781 gagttaaaaa tagaaaatat tgaaaatata ctattaattg aaaaaagtga taattttttt
      841 aatactatta gaacttttta taaagatggt aaaagagttg ataaatattg gcgtaatcaa
      901 actattaacc ttattggaaa tatccctttt gatggtggat taaaagaaga tgtattaaac
      961 tattttgaaa gccttttaaa aaaatataat atacctacaa tttataacaa tgaagtagaa
     1021 aaagttataa aacttgataa tggaattttt gaagttcaca ctaagttaga tatttataaa
     1081 gcaaaaaatg ttgtaattgc tattggaaag atgggaaaac caaataaacc taactataaa
     1141 atagcttcag agattcaaaa aaatataaat tttaatcttg atagctgttc aaaaaatgag
     1201 aaaatattag ttgttggtgg tggaaatagt gctgctgagt atgcatattc tctagctttg
     1261 gatgataata ttgtaacttt agcatataga aaagagagtt ttacaagatt aaataaaatt
     1321 aatgaaaata cgttaaaaga gtttgaagct aataaaaaat taactataaa attaaattgt
     1381 gatataaact ctttagaaga tgataatgga cttgtaaaag taaacttctc aaatgctgag
     1441 agtgaaaatt tcaatagagt aatttatgct attggaggaa cagctcctac tgattttcta
     1501 aaaaattgtg gtgtaaaatt tagtggtaat ttaaaaccaa tttttgatga aaactttgag
     1561 tgttgttgta aaaatttata tatagctgga gatgttaatt cttcaagtga aggttcaatt
     1621 tcaagcagtt taaatcacgg atttttaatt tcaaaagcaa tagctaaagg tttaaaacct
     1681 ttagcttaaa catcaagatg ttctacatct tttgcgtgag attctatata gtttcttcta
     1741 ggttctactt catcacccat aaatagagta aatgtatcgc ttgcagcttc tgcatcttct
     1801 attttcactc ttaaaagtct tctattttca ggtgtcatag ttgtctccca aagttgttca
     1861 ggattcatct ctccaagacc tttatatctt tgaatataag cacctttttt tgctaaactt
     1921 tcaatctctt caagaagttc aattaaatct ctaccttcaa atagtgttaa atctctctct
     1981 tttaacttag taaatataaa agttgattca ctaaaataag gtgatgcaaa tagctcttcg
     2041 tctattatta actcttcaag tcctgcattt gtctgaacaa aaagttgaat ttttgtttca
     2101 gtaatagttt ttgaaagtat gttataacct cgtgcttcta agaacagttt aactttttca
     2161 tataaagtag gaaactctaa atttactaaa tcactatttt caattagata ttttaaaact
     2221 tcaatcaaag agtatctttt tgctaattgt tctaacatag ctctatatct tgctacttgt
     2281 ttaaacagat caagtaaatc attatatccc ataccttcaa actcaaaatt ttcaagtcca
     2341 ttttcaatta aataagcaga taatgcatta ttatctttta aataaatctc atttttacct
     2401 ttttt
//
LOCUS       DAOQAN010000132         2399 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_63730_length_2399_cov_3.018771, whole genome shotgun sequence.
ACCESSION   DAOQAN010000132 DAOQAN010000000
VERSION     DAOQAN010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63730_length_2399_cov_3.018771"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..1274)
                     /locus_tag="PKO10_02945"
     CDS             complement(63..1274)
                     /locus_tag="PKO10_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNW66140.1"
                     /translation="MLLNALLIAIKEIRRNILRSILTILGIVIGVASVIAMVMIGDGT
                     TANVTANIEKLGSNMLNVRVGQEKRGAPRDDNSAKPFKIEDITAIKNEVANLKGVTAE
                     NSGMANVVFGNKSNSSLIVGTTNDYFIIKDWEVEQGRIFEEGELSSGKSVCILGTTVV
                     KNLFGDENPIGATIRIKNFPCSVIGVLASKGASSFGRDQDEVVITPLKMYQRKIQGNL
                     DVSTIIVSVMEEKYIEDAKAQIISLMQDRRAIKLGEADNFHIRDMKDILNTMTSTTKM
                     LTYLLGSIAAISLLVGGIGIMNIMLVSVTERTREIGIRLAIGAMQSEVLLQFLIEAIV
                     LSTWGGIIGILLGLGVGYGVVQIFELPYIINTQIILISFIFSTLIGVVFGYFPARKAA
                     RLNPIDALRYE"
     gene            complement(1274..1978)
                     /locus_tag="PKO10_02950"
     CDS             complement(1274..1978)
                     /locus_tag="PKO10_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNW66141.1"
                     /translation="MKNSEVLIEFKNIKKIYGKGANETFALNGVDLKIHKGEFVAIMG
                     ASGSGKSTSMNIIGCLDKPSSGEYLFDGVNVENLNLNQMAILRRNYIGFVFQGFNLLG
                     RTTALENVELPLVYRKVPKEQRNKLAIEALKKVGLESVIKHTPAELSGGQQQRVAIAR
                     AIVTDPLLLLADEPTGNLDSIKSIEVMELLKKLNEELNITIIMVTHEEEMAAYASRVI
                     YFRDGNIEDSLKKGFK"
     gene            complement(1985..>2399)
                     /locus_tag="PKO10_02955"
     CDS             complement(1985..>2399)
                     /locus_tag="PKO10_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014473550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNW66142.1"
                     /translation="NPITTNGVVTYETVVSVDNSELLLKPGMTANAKIITKNIKDQIL
                     IPNSALRFTPKNSTEKTATKPASFGTPPNFRAQGSVTKDNKAKDGFATIYILESGKPK
                     ELKVKVLDSDSKQSSIFSETLKIGDEVIISQKSGN"
BASE COUNT          773 a          447 c          250 g          929 t
ORIGIN      
        1 ttataaatga ttagaaatat caaatgcaaa tttattacaa aaaactctgt tttgattcta
       61 ttttattcat atcttaaagc atctattgga tttaatcttg ctgcttttct tgcaggaaag
      121 tatccaaaaa caactccaat caaagttgaa aatataaaag atattaaaat tatttgagta
      181 tttataatat atggtaattc aaatatttgt acaactccat aaccaacacc aagcccaagt
      241 aaaattccaa taattccacc ccaagttgaa agaactattg cttcaattag aaattgaagt
      301 agaacttcac tttgcatagc accaatagca agtcttattc caatctctct agttctttca
      361 gttacagaaa caagcataat attcataatc ccaattccac caacaagtaa agagatagca
      421 gcaattgaac ctagcaaata tgttagcatt tttgttgtgc ttgtcatagt atttaatata
      481 tctttcatat ctcgtatatg aaaattatcc gcttctccta attttatagc tcttctatct
      541 tgcattaatg aaattatttg tgcttttgca tcttcaatat atttttcttc cataactgat
      601 acgattattg tagatacatc taaatttcct tgaatctttc tttgatacat tttaagaggt
      661 gttattacaa cctcatcttg gtctcttcca aaactactag ctccttttga agctaaaact
      721 ccaattacac tacaagggaa attttttatt ctaatagttg cacctattgg attttcatct
      781 ccaaaaagat ttttaacaac tgttgtcccc aaaatacaaa cactttttcc acttgaaagt
      841 tcaccttctt caaaaattct tccttgttca acttcccaat cttttataat aaaataatca
      901 tttgtagttc caacaattaa agatgaatta cttttatttc caaaaacaac attcgccata
      961 ccagagtttt ctgctgttac accttttaaa tttgcaactt cattttttat agcagttata
     1021 tcttcaattt taaaaggttt tgcactatta tcatctcttg gagctcctct tttttcttgt
     1081 ccaactcgaa catttagcat attgcttcca agtttttcta tatttgctgt tacatttgct
     1141 gttgttccat ctccaatcat aaccatagca ataacagaag caacaccaat tacaattccc
     1201 aaaatagtaa gaattgatct taatatattt cttcttatct cttttattgc tattaataaa
     1261 gcatttagca acattattta aatccttttt ttaaagaatc ttctatattt ccatctctaa
     1321 aatatataac tcttgaagca taagcagcca tctcttcttc atgtgttacc ataataatag
     1381 taatatttaa ctcttcattt aattttttta gtaactccat aacctcaata cttttaatac
     1441 tatcaagatt tccagtaggt tcatctgcca aaagtaaaag tgggtctgta acaatagcac
     1501 gtgcaattgc aacacgttgt tgctgaccac cactaagctc tgctggagta tgtttaataa
     1561 cactttcaag accaactttt tttaatgctt ctattgctaa tttatttctt tgttcttttg
     1621 gaacttttct atatacaaga ggtaattcaa cattctccaa agcagttgtt cttcctagta
     1681 gattaaatcc ttgaaaaaca aaacctatat agtttcttct taaaattgcc atttgattta
     1741 gatttaaatt ctctacattc acaccatcaa acaaatactc gccactactt ggcttatcca
     1801 aacatccaat aatattcata gaggtagatt ttccacttcc actagctccc ataatagcta
     1861 caaattcacc tttatgaatc tttaaatcca ctccatttaa agcaaaagtc tcattcgcac
     1921 ctttaccata aatttttttt atatttttaa attcaattaa aacttcacta tttttcataa
     1981 atttctaatt tccagatttt tgtgaaataa ttacttcatc accaattttt aaagtttcag
     2041 aaaaaataga tgattgtttt gaatcactat ctagaacttt aacttttaac tcttttggtt
     2101 ttccagattc aagaatataa atagttgcaa agccatcttt tgctttatta tcttttgtaa
     2161 cacttccttg agctctaaaa tttggtggtg ttccaaaact agctggtttt gtagctgttt
     2221 tttcagtact attttttgga gtaaatctta atgcactatt tggaattaaa atctgatctt
     2281 ttatattttt tgtaataatt tttgcatttg ctgtcattcc tggttttaaa agaagttcac
     2341 tattatcaac acttacaaca gtttcataag ttactacgcc atttgtagta attgggttt
//
LOCUS       DAOQAN010000133         2382 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_64460_length_2382_cov_2.427159, whole genome shotgun sequence.
ACCESSION   DAOQAN010000133 DAOQAN010000000
VERSION     DAOQAN010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64460_length_2382_cov_2.427159"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1170)
                     /locus_tag="PKO10_02960"
                     /pseudo
     CDS             complement(1..1170)
                     /locus_tag="PKO10_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012317.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
     gene            1357..>2382
                     /locus_tag="PKO10_02965"
     CDS             1357..>2382
                     /locus_tag="PKO10_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNW66143.1"
                     /translation="MSVKHYEVIVVGGGISGAALFFELAKYSDINNICLLEKYDDLAT
                     LNSKGTSNSQTIHVGDIETNYTFDKAKITKRTAKMIEKFNLMYNLQDKIMFKHQKMAL
                     GVGEKEVKFITDRYNQFKEIFPYLELWDKETLREKEPLLVYADKERTKDRPEPIIAMG
                     TNDQYTTVDFGAMTKELVKAAKEADSSKTTDVFFNQEVDEIEKIGDKFKVTTTNGTVY
                     TADFVVVNAGAHSLYLAHKMGHGKHMGSLSMAGSFYITNGTYLNGKVYMVQNDKLPFA
                     ALHGDPDILCDGKTRFGPTALALLVLERYKGGKSFFQCLKTLNFDGSIMKIFWDLLKD
                     SDIRNYVF"
BASE COUNT          844 a          355 c          329 g          854 t
ORIGIN      
        1 agagtgtcta atatcttctc tttttaataa agaacttcct aactcttcta aaattaactc
       61 atttgccttg aatacattta aatctaatta tttatttgta tctttaaata gtttttctct
      121 tactatcgtt tcatcatatt taccatatgc gtccactggc ataattacat ctaagttata
      181 tcttaatcca actttatagt catcttctcc atgtccaggt gcagtatgaa ctgctccagt
      241 tccactatcc atcataacat ggtcacctaa aacaattttt gaagttcttc cattcaatgg
      301 attaattgca aatgtatttt ctaaatcttt tggattaata gtttcaacta cttcgccact
      361 tataactgca ctttctatta aagagttata taatttttta gcaactataa atttatcaga
      421 agtttttaca tattcttcat caccatttaa agaaattcct gtatttgcag gaagtgtcca
      481 aggtgttgtt gtccaaataa taatactagc atcaagatct tgatgtttaa atgccacata
      541 aattgaaggt gaagttttat cttcatactc aacttcagct tcagctaaag cagtttgagc
      601 agcccaagac caataaacag gtttacttct ttgaactaaa agcccttgtt tagcaattgc
      661 acaaagctct ctataaatat ttgcttcaaa tttaaaatcc atagttaaat aaggattttc
      721 ccaatcagcg ataactccaa gttgtttaaa ttcatctttt tgaatttcaa caaattttgt
      781 tgcatgatct ctacaaagtt gtcttaattt tgattttgga agctcttttt tcttctcact
      841 tcctattttt tcttcaactt tttgttcaat tggtaaacca tgacaatccc aacctggagt
      901 atatcgtata gattttccat caaaatagtg aaatttgttt ataatatctt ttaaaatttt
      961 atttaaagca tgacctatat gaatatttcc atttgcataa ggaggtccat catgaagagt
     1021 aaaactttca caccctactc tatttttctt cattttttca tatacagctt gttcatccca
     1081 cttcttgtat cttattggtt cattttgtgg caaaccacct ctcattggaa aatcagtttt
     1141 tggaagcaac aaactctctt tataatccat atttcacctc tagtttaaat aattttctat
     1201 tttgttaaat cgaagattat atctaaatat atttaaaata aaattttatt atctttactt
     1261 aaaaatttaa taatttaaca caatctttat gtgaaataaa aaaattatat gctagaatat
     1321 ttgccaaatt ttgacaaaat aaatatagga tagaaaatga gcgtaaaaca ctatgaagta
     1381 attgttgttg gtggtggaat ttctggagct gctttgtttt ttgagttagc aaaatattct
     1441 gatataaata atatttgttt acttgagaag tatgatgatc ttgctacact taactctaaa
     1501 ggtacaagta actctcaaac aatacatgtt ggagatattg aaacaaacta tacttttgat
     1561 aaagcaaaaa ttacaaaaag aacagcaaag atgattgaga aatttaatct tatgtataat
     1621 ttacaagata aaattatgtt caaacatcaa aaaatggctc ttggagttgg agaaaaagag
     1681 gttaaattta ttacagatag atataatcaa tttaaagaga tttttccata tttagaactt
     1741 tgggacaaag aaactttaag agaaaaagag ccattacttg tttatgcaga taaagagaga
     1801 acaaaagata gaccagaacc aattatagct atgggaacaa atgatcaata tacaactgtt
     1861 gattttggag ctatgacaaa agaacttgta aaagctgcaa aagaggcaga tagctctaaa
     1921 acaactgatg tattttttaa tcaagaagta gatgaaatag aaaaaattgg tgataaattt
     1981 aaagttacaa ctacaaatgg aacagtttat acagctgatt ttgttgttgt aaatgcagga
     2041 gcacattcac tttatttagc tcacaaaatg ggacatggaa aacatatggg ttcattatca
     2101 atggctggat cattttatat tacaaatgga acatatttaa atggtaaagt atatatggtt
     2161 caaaatgaca aacttccatt tgcagcactt catggagatc cagatatttt atgtgatgga
     2221 aaaacaagat ttggaccaac agcattagct cttttagtgc ttgaaagata caaaggtgga
     2281 aaatctttct tccaatgttt aaaaacttta aacttcgatg gaagtattat gaaaattttc
     2341 tgggatttac taaaagatag cgatattaga aattatgtat tt
//
LOCUS       DAOQAN010000134         2375 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_64768_length_2375_cov_2.736207, whole genome shotgun sequence.
ACCESSION   DAOQAN010000134 DAOQAN010000000
VERSION     DAOQAN010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64768_length_2375_cov_2.736207"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..810
                     /locus_tag="PKO10_02970"
     CDS             <1..810
                     /locus_tag="PKO10_02970"
                     /inference="COORDINATES: protein motif:HMM:NF025854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNW66144.1"
                     /translation="LEKRIKKEIEKSKAKDKIHYQEQLKSAKLSYIGVLSAGITHEIN
                     TPLTYVKGNLELMHYDIESLDDSDIKNRMLQDSKKIKEGLNRIANIVESMREISHSNE
                     GEKRDFNVYATILTALTMAHNRAKHISKIYLNGEIFDINNIDNNRFIFMSNIQKQRIE
                     QIWIIIINNALDELVKIEDYENRELKIDILEEKNNILVRFKDNAGGLSKEILKKLFEP
                     FTSSKNQGGMGIGLSIAKKIVEEHNGSIKAYNEDNGALFEVRLRKIKEDKV"
     gene            807..1631
                     /locus_tag="PKO10_02975"
     CDS             807..1631
                     /locus_tag="PKO10_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HNW66145.1"
                     /translation="MRIGFIGDIVGRPGRKIIKDSLKNIKEEHKIDFIIANGENASHG
                     FGLTIDSSKELFSSGIDLITGGNHSFDKKKDVQYLHESGNVLRPDNYPEGVVGSGFKI
                     CDIKIEERVEKLAVINLMGQFAMPLVENPFNWATKLIKNLEEQNIKNIFVDFHGEATS
                     EKRVMLMMFKNRVSAICGTHTHVGTDDLQIFENTAYLTDIGLTGCYDNVIGMDEKAPI
                     KRATTGIGSHFEVPNSCKSILQMMVVDIEDGKAINSYKIKKYCNSSKLHISDAIII"
     gene            1682..>2375
                     /locus_tag="PKO10_02980"
     CDS             1682..>2375
                     /locus_tag="PKO10_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014473278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66146.1"
                     /translation="MIIIPQTKGGVGKSTVAMQVIAPYLYKKHGKKITYIEIDDENND
                     SQSFTRTEIVNKRMLGTNRITDLDELILMDDKHEVIVDVGGNKTSSLVLDEIKKVGSF
                     GNVKWIIPLGDGELDGKNAIATMKKIRKIEKNPEDNIIFALTRAISMEEDYYSEQFIN
                     FFGHKYLDSNSVICDFVKNPKYFPVKNDKIITMSRYLGSTVWEMAYNNTDFAKKAIEA
                     KELGDVEAARKYL"
BASE COUNT          997 a          209 c          442 g          727 t
ORIGIN      
        1 cttgaaaaaa gaataaaaaa agagatagaa aagagtaaag caaaagataa aatccattat
       61 caagagcagt taaaaagtgc aaaattgagt tatattggtg ttttatctgc tggaattaca
      121 catgaaataa atacaccttt gacatatgta aaaggaaatt tagagcttat gcattatgat
      181 attgaatctc ttgatgattc agatataaaa aatagaatgt tgcaagatag taaaaaaata
      241 aaagaggggt taaatagaat tgcaaatata gttgaaagta tgagagaaat ttctcattca
      301 aatgagggag aaaaaagaga ttttaatgta tatgccacta tacttacagc tttgactatg
      361 gcacataata gagcaaaaca tattagtaaa atatatttaa atggtgaaat ttttgatata
      421 aataacattg ataacaatag atttattttt atgagtaata ttcaaaaaca aagaatagag
      481 cagatttgga ttattattat aaataatgct cttgatgaat tagtaaaaat tgaagattat
      541 gaaaatagag agctaaaaat tgatatttta gaagagaaaa acaatattct tgtaagattt
      601 aaagataatg ctggtggctt aagcaaagag atactaaaaa aattgtttga accatttaca
      661 agttctaaaa atcaaggtgg aatgggaata ggattatcta ttgccaaaaa aatagtagaa
      721 gagcataatg gtagtataaa agcttacaat gaagataatg gagcactttt tgaagtaagg
      781 cttagaaaaa taaaagagga taaggtttga gaataggatt tataggtgat atagtaggtc
      841 gtccaggaag aaagattata aaagatagtt taaaaaatat taaagaagag cataaaatag
      901 attttataat agcaaatggt gaaaatgcaa gtcatggttt tgggcttaca attgatagtt
      961 caaaagagtt attttctagt ggaattgatt taattacagg tggtaatcat agttttgata
     1021 agaaaaaaga tgttcaatat ttacatgaaa gtggaaatgt tttacgacca gataattatc
     1081 cagaaggtgt agttggtagc ggttttaaaa tatgtgatat taaaattgaa gaaagagttg
     1141 aaaaattagc agttataaat cttatgggac aatttgcaat gcctttagtt gagaacccat
     1201 ttaactgggc tacaaaatta ataaaaaatt tagaggaaca aaatattaaa aatatttttg
     1261 tagattttca tggtgaagca actagtgaaa aaagagttat gcttatgatg tttaaaaaca
     1321 gggttagtgc aatttgtgga acacatactc atgtaggaac agatgattta caaatttttg
     1381 aaaatactgc atatttgaca gatattggat taactggttg ttatgacaat gttataggaa
     1441 tggatgaaaa agcaccaatt aaaagagcaa caacaggaat aggtagtcac tttgaagttc
     1501 caaactcttg taaatcaata cttcaaatga tggttgtaga tatagaagat ggaaaagcta
     1561 tcaactctta taagataaaa aaatattgca atagcagtaa attgcatatt agtgatgcaa
     1621 taattattta atgatagttt agataaaatc aaagaatatt tgtttaaaaa aggcttataa
     1681 aatgataata attcctcaaa caaaaggtgg agtaggtaaa tctactgttg ctatgcaggt
     1741 tattgctcca tatttgtata aaaaacatgg taaaaaaatc acttatatag aaattgatga
     1801 tgaaaataat gattcacaat cttttacaag aactgaaata gtaaacaaaa gaatgctagg
     1861 aacaaataga attacagatt tagatgaact tatattgatg gatgataaac atgaagttat
     1921 tgtcgatgtt ggtggaaata aaacttcaag tttggttcta gatgagataa aaaaagttgg
     1981 aagttttgga aatgttaagt ggattattcc tttgggagat ggtgaacttg atgggaaaaa
     2041 tgcaattgca actatgaaaa aaattagaaa aatagagaaa aatccagaag ataatattat
     2101 ttttgcactc acacgtgcta tatctatgga agaggattat tatagtgagc agtttataaa
     2161 cttctttgga cataaatatc ttgactcaaa ctcggttatt tgtgattttg taaaaaatcc
     2221 aaaatatttt cctgttaaaa atgataagat tattacaatg agcagatatt taggaagtac
     2281 agtttgggaa atggcttaca ataatacaga ttttgctaaa aaagctattg aagcaaaaga
     2341 gttaggagat gttgaagctg ctagaaaata tctat
//
LOCUS       DAOQAN010000135         2372 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_64879_length_2372_cov_3.339232, whole genome shotgun sequence.
ACCESSION   DAOQAN010000135 DAOQAN010000000
VERSION     DAOQAN010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64879_length_2372_cov_3.339232"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..822)
                     /locus_tag="PKO10_02985"
     CDS             complement(<1..822)
                     /locus_tag="PKO10_02985"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin fold domain-containing protein"
                     /protein_id="HNW66147.1"
                     /translation="MKFIFRRLFLIFVAIFFATTLNAKEGKVIGSSNHEVPSWFKDSF
                     LDISEDIEEATEKNKHFMIFLDFEGCPYCSKMLKESFEEDNKTSQFIKNNFDVIELNV
                     KGSRELTWVDEDVLTEKDLTEKLKIQYSPTLLIFNSNKEIVARVNGYRNSKDFQYILD
                     FIQTKSYEKMDFTSYLSKLDKKDTYIFIPNKMFKDLNDLSKIKTPVAIIFEDKSCIQC
                     DHFHNKILTNKDVIDEFSKFTVIRVDANSTKELILPTGEKTTPKSLVEKINLDYRP"
     gene            1031..>2372
                     /gene="ccoG"
                     /locus_tag="PKO10_02990"
     CDS             1031..>2372
                     /gene="ccoG"
                     /locus_tag="PKO10_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014469512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HNW66148.1"
                     /translation="MSEKNEFVKKTSYRKKRYFVYAIVSIISLVMPFIQINGNQLFLL
                     SFDKKQFHLMGTAFDMQELYLMPFLLMLLFIGIFAITSIGGRAWCGWACPQTIFRVIY
                     RDLIESTLLKLRRIKNKQKDIDLSKNENKIKKFVGVILWSILSLVAASNFMWYFVPPQ
                     DFFAYIQDPLEHMFLIGIVLSIAAFLIYDVIILKEDFCVYICPYSRVQSVLYDNNTYQ
                     AIYSTKRGGKIYDENKEKVVWKIKDLPDPANECTACEACVTVCPTHIDIRKGLQLECI
                     NCLECVDACTTVMGKLGKPSLVQWSSTNTIVENKPTKLLRKTTIMYFISLTITFALLF
                     AMSGEKEYMLLNVNKDTELYKIKDGNVVSNNYLLLFQNTESKTLTYELEIVDNPDIKI
                     TRFEPFTLSPGKMAKKVVILETDKLLVDNNLKDTPITVTIKAYAKEDPEKVKVFR"
BASE COUNT          887 a          300 c          295 g          890 t
ORIGIN      
        1 tggtctataa tctaaattta ttttctcaac caaagatttt ggtgtagttt tttcacctgt
       61 tggtaaaatt aactcttttg tactatttgc atcaactcta ataactgtaa attttgaaaa
      121 ctcatcaatt acatctttat ttgttagaat tttattgtga aaatggtcac attgaataca
      181 acttttatct tcaaaaataa ttgcaacagg agtttttatt ttagataaat catttaaatc
      241 tttaaacatt ttatttggta taaaaatata tgtatctttt ttatctaact tacttaagta
      301 tgatgtaaaa tccatttttt cataagattt tgtctgtata aaatccaaaa tatattgaaa
      361 atctttacta tttctatatc catttactct agctactatc tctttatttg agttaaaaat
      421 aagaagtgtt ggactgtatt gaatttttaa tttttctgtt aaatcttttt cagttaagac
      481 atcttcatca acccaagtta attcacgact tcctttaaca tttagttcaa taacatcaaa
      541 attgtttttg ataaattggc tagttttgtt atcttcttca aaactctctt ttagcatttt
      601 tgaacaataa ggacaacctt caaaatccaa aaatatcata aaatgcttat tcttttcagt
      661 ggcttcttca atatcttcac ttatatctaa aaaactatcc ttaaaccaac taggtacttc
      721 atgatttgaa gaaccaataa ctttcccctc ttttgcattt aaagttgtag caaaaaatat
      781 tgcaacaaaa attaaaaata aacgtctaaa tataaacttc ataatctctc ctatatttaa
      841 aataataatg attctattca aattatcatt ataaaaagct ataaatttga attctttaat
      901 ctaaaaatga tagaattctt tttataaaat aaaatttact ataaatataa tttaatctta
      961 tgataaatgt ctataagaat aaattgtgtt tatataaaat cttaatattt tttatgtaaa
     1021 aggatttaaa atgagcgaaa aaaatgagtt tgtaaaaaaa acttcttatc gtaaaaagag
     1081 atattttgtt tatgccattg tttcaataat atcattagta atgccattta ttcaaataaa
     1141 tggaaatcaa ttattcttat tatcttttga taaaaaacag tttcatctta tgggaactgc
     1201 ttttgatatg caagagttat acttaatgcc atttttattg atgttgttat ttataggaat
     1261 ttttgcaata acttcaatcg gtggtagagc ttggtgtggt tgggcatgtc ctcagacgat
     1321 ttttagagta atttatagag atttaattga atcaacactt ttaaaactta gaagaataaa
     1381 aaataaacaa aaagatattg atttaagtaa aaatgaaaat aaaatcaaaa aatttgttgg
     1441 tgtaatttta tggtctattt tatctttagt tgcagcatca aactttatgt ggtattttgt
     1501 accgccacaa gatttctttg catatattca agacccactt gaacatatgt ttttaatagg
     1561 aattgtttta agtatagctg catttttaat ctatgatgta attattttaa aagaagattt
     1621 ctgtgtatat atctgtcctt attcaagagt tcaatctgtt ttatatgata ataatactta
     1681 tcaagcaatc tactctacaa aaagaggtgg aaaaatatat gatgaaaata aagaaaaagt
     1741 tgtttggaaa ataaaagatt taccagaccc agcaaatgag tgtactgctt gtgaagcttg
     1801 tgttactgtt tgccctactc atattgatat tagaaaaggt ttacaactag agtgtataaa
     1861 ctgtcttgag tgtgtagatg cttgtacaac agttatggga aaacttggaa aaccatcttt
     1921 agttcaatgg tcaagtacaa atactatagt tgaaaataaa ccaacaaaac ttcttagaaa
     1981 aacaacaatt atgtatttca tctctcttac tatcactttt gctctacttt ttgctatgag
     2041 tggagaaaaa gagtatatgc ttttaaatgt taataaagat acagaacttt ataagataaa
     2101 agatggaaat gttgtatcaa ataactattt attacttttc caaaatactg agtcaaaaac
     2161 tctaacttac gaattagaaa tagtagataa tccagatata aaaatcacaa gatttgaacc
     2221 atttactcta agtcctggaa aaatggcaaa aaaagttgtg attcttgaaa cagataaact
     2281 tttagtagat aataatctaa aagatactcc aattacagta actattaagg cttatgctaa
     2341 agaggatcca gaaaaagtaa aagtatttag aa
//
LOCUS       DAOQAN010000136         2371 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_64932_length_2371_cov_2.953800, whole genome shotgun sequence.
ACCESSION   DAOQAN010000136 DAOQAN010000000
VERSION     DAOQAN010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64932_length_2371_cov_2.953800"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1178)
                     /locus_tag="PKO10_02995"
                     /pseudo
     CDS             complement(1..1178)
                     /locus_tag="PKO10_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510341.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
     gene            complement(1175..1960)
                     /gene="murI"
                     /locus_tag="PKO10_03000"
     CDS             complement(1175..1960)
                     /gene="murI"
                     /locus_tag="PKO10_03000"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HNW66149.1"
                     /translation="MKVGVFDSGLGGLTVLNAIIKQLKGAEIFYIADTLYAPYGDKNK
                     DEIFSRSEKITNFLLKNYEIDALVIACNTATSVAIKHLREVFPSLIIIGTEPGIKPAI
                     NNTKTKNIGILATASTLKGEKYQLLANELTTKSEVTLHEQACIGLVEQIENGEINSLK
                     TYAMLENWLKPMLHNKVDTIVLGCTHYPLVKKTILDIVGEDVLLIDTGDAIAKRVVAL
                     SQDLQNHNKNALNIEILYTGKINSDMVKIILGKNKFEMRKCEL"
     gene            complement(2193..>2371)
                     /locus_tag="PKO10_03005"
     CDS             complement(2193..>2371)
                     /locus_tag="PKO10_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HNW66150.1"
                     /translation="VSWTFEEVDAKLEKIMKHIFDTASATAKEFGEPTNLVLGANIAG
                     FRRVADAMIEQGIV"
BASE COUNT          770 a          377 c          285 g          939 t
ORIGIN      
        1 attttcaatt ttagaatctt ctaaaataac ttcttcttta taatttgact cttttttaat
       61 ttccgatttt aattctatat ttatatcact cttaatttca ggttttgtag gaattttttc
      121 tatttgttct ataatttcat ctatactttt taagttcgtt gcttcaatta gtttaagcaa
      181 agttagaatt aatacaaacc catcatcact atttaaagct aaaagatatt ttgcttcact
      241 taaaattcta aaaaatctat caaataaagt aatatcaaat ctaacatctt ttgaaatcat
      301 cttatctttt aaaaagattg caatctcatc acaaatttga ccaacttcat atgattgaag
      361 ctcccaaagc aagggattta tatcttcttt atttaagatt actttaaata ttttttccat
      421 taaagctgga tttattaatc ctagcatatc aacgacacta gaggttgcaa ctcttccttt
      481 tgagaaaata atagcttgat caagtagagt caaagtgtct cttaagcttc cttgtccact
      541 tcttgcaagt atttctaagc atctttttca aattcaatat tttctatatt taaaatatga
      601 gaaagatgat gaactacatc actttgagat attttattaa atctaaagtg ttgagttcga
      661 ctaagaattg ttgctggaag ttttaaagca tcagttgttg caagtataaa ttttacaaaa
      721 gctggaggct cttctaaagt tttaagcaga gcattaaaag cttgagttgt aagcatatga
      781 acttcatcta taataaaaac tttatatctt gcaatattag gtttgtattt tgtatgctct
      841 attaactctt ttatatcatc aattcctcta ttacttgccg catccatttc aataatatca
      901 agatgtttcc cactttttgc actaaggcag ttttcacaaa cttcacaagg gtggctagtt
      961 ggtccatttt cgcataaaag tgctttagcc ataattctag ctgttgaagt ttttccactt
     1021 cctctaagtc cagaaaacaa atatgcatga gataatcttt tactatccaa agcaagactt
     1081 aaagtttgag aaattgtatt ttgtcctatt aggtcttcaa atttttcagg tctgtattta
     1141 agagctaaaa ctctatcatc ttttatattt ggactcataa ttcacatttc ctcatttcaa
     1201 atttgttttt tcctaaaatt atttttacca tatctgaatt tatctttcca gtgtataaaa
     1261 tctctatatt taaagcattt ttattatggt tttgtaaatc ttgacttaga gctacaactc
     1321 tttttgcaat tgcatcacct gtatcaatta aaagaacatc ttctcctaca atatcaagaa
     1381 ttgttttttt tactaaagga taatgtgtgc aacctaaaac aattgtatca actttgttgt
     1441 gaagcattgg ctttagccaa ttttcaagca tagcgtaagt ttttaaacta tttatctcac
     1501 cattttcaat ctgttcaact aaaccaatac atgcttgttc atgcaaagtt acttcacttt
     1561 ttgttgttaa ttcatttgct agaagttgat atttttcacc ttttagagtt gaagctgttg
     1621 ctagaatacc aatatttttt gtttttgtat tatttattgc tggttttatt ccaggttcag
     1681 taccaataat tattaaagag ggaaaaactt ctcttaaatg ctttattgca actgatgtag
     1741 cagtattgca agcaattaca agtgcatcta tttcataatt ttttaataaa aaatttgtaa
     1801 ttttttcaga tcttgaaaat atttcatctt tatttttatc accataaggt gcatataaag
     1861 tatcagctat ataaaatatt tcggcacctt ttagttgttt aataattgca tttagtacag
     1921 ttaatccacc aagacccgaa tcaaaaactc ctactttcaa ctttttcctt actgaaaatt
     1981 tgcgaaatta taaccaaaaa catataacta aaatgttaag caagaactct ttaggaatgt
     2041 ttttgtttat aatttgaaaa cttaaaattt taattgaata aaaggaaaat aaattttaag
     2101 ttttctaaat gattattatg tggttaaagg gtttatatat aaaagtaagt ttttggtaaa
     2161 cctaaggaaa aatccttaag ttctattttt tatcaaacaa ttccttgctc aatcatagca
     2221 tcagctactc ttctaaatcc agcaatattt gctcctaaaa caaggtttgt tggctctcca
     2281 aactcttttg cagttgcgct tgctgtatca aaaatatgtt tcataatttt ttctagtttt
     2341 gcatcaacct cttcaaaagt ccaagaaacc a
//
LOCUS       DAOQAN010000137         2364 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_65209_length_2364_cov_3.866609, whole genome shotgun sequence.
ACCESSION   DAOQAN010000137 DAOQAN010000000
VERSION     DAOQAN010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65209_length_2364_cov_3.866609"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="PKO10_03010"
     CDS             complement(<1..411)
                     /locus_tag="PKO10_03010"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HNW66151.1"
                     /translation="MDFFTNENIIFYLLAYLIGSIPFGLILAKTFAGVDIKNDGSRSI
                     GATNVLRVVKKTNPSLAKKLGIATVILDALKGTILLLVGMFIFNLSEQTLWAMAVLSV
                     LGHCYSIYLGLEGGKGVATGLGVYIVLIPISTLIG"
     gene            503..1492
                     /gene="nadA"
                     /locus_tag="PKO10_03015"
     CDS             503..1492
                     /gene="nadA"
                     /locus_tag="PKO10_03015"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HNW66152.1"
                     /translation="MNYKEEILKLKKELDVVLVAHFYQRDEVFELADITGDSLELAKK
                     VMESDKEFVVFCGVGFMGESAKILNPQKRVLMPRIACCAMARMIDEGYFLENLKKMNE
                     AGISNDDILPITYINSSASVKARVGEMGGMVCTSSNAYKIIEKGLKSGKKIFFVPDRC
                     LGQNFAKSLGLKSAVIGDGTNLKEADIICYNGFCSVHQQFNIDDIDFYREKYPGIIVA
                     VHPECDPSVCDKADFVGSTSQLIKFIKELPEDQKVVVGTEFNMVNRLREKNTYILSST
                     KPECPTMNETTLEHVYLTLKSIKENKISELSEIKVDEKTRYWAKIALERMFEI"
     gene            1489..2310
                     /gene="nadC"
                     /locus_tag="PKO10_03020"
     CDS             1489..2310
                     /gene="nadC"
                     /locus_tag="PKO10_03020"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HNW66153.1"
                     /translation="MIHIKRFVKQAITEDNGRGDLFYDIAPDGKFTAKIVSKSNGILA
                     GVKYAETLAQTEKIKVDFLKKDGDKIQAGDILAKLEGKASKLLSSERTFLNMLQHASG
                     IATMASKFVEKLDGFDVVLLDTRKTRPHLRDFEKYASRVGGAINHRLGLDDCLMLKDT
                     HLRTIENLAEFVKIARKRISWVTKIEIECETFSQVETAMEAGADIIMCDNMTFQQIKE
                     VVKYRDQKYPHILIEASGNINLDTIQEYAKTGVDAVSSGSIIHQATWLDFSMRVD"
BASE COUNT          918 a          276 c          415 g          755 t
ORIGIN      
        1 accaattagt gtagaaattg gaattaaaac aatataaact ccaagtcctg ttgctacacc
       61 ttttccgcct tcaagcccta aataaataga gtagcagtgc cctaaaacac ttaatactgc
      121 cattgcccaa agagtttgct cacttaaatt aaagataaac ataccaacta aaagtaaaat
      181 tgtacctttt aaagcatcta aaattactgt tgcaatgccc aatttttttg ctaaactagg
      241 atttgttttt tttactactc ttaaaacatt tgttgcacca atacttctac taccatcatt
      301 ttttatatca acaccagcga aagtttttgc tagtattaaa ccaaaaggaa tagaacctat
      361 taaatatgct aaaagataaa aaattatatt ttcatttgta aaaaaatcca tcaataacct
      421 ttgttgtttt taaaattaaa tgagattata actaaatttt tgatatattc tcacttaata
      481 aaatttttaa aaggtaatat tattgaatta taaagaagag attttaaaac taaaaaaaga
      541 gctagatgtt gtacttgtag ctcactttta ccaaagagat gaagtatttg aattagcaga
      601 tataactggt gatagccttg aattagcaaa aaaagttatg gaaagcgaca aagagtttgt
      661 tgtattttgt ggagttggat ttatgggaga gagtgcaaaa attttaaatc cacaaaaaag
      721 agttctaatg cctagaattg cttgttgtgc aatggctaga atgattgatg aaggttattt
      781 tttagaaaat ttaaaaaaga tgaatgaagc tggaatatca aatgacgata ttttaccaat
      841 tacttatatc aattcaagtg caagtgtaaa agctagagtt ggagagatgg gtggaatggt
      901 atgtacatct tcaaatgctt acaaaataat tgaaaaaggt ctgaaatctg gtaaaaaaat
      961 attttttgta cctgatagat gtttaggaca aaattttgca aaaagcttag gattaaaatc
     1021 agcagttatt ggggatggaa caaatttaaa agaagctgat attatttgct ataacggttt
     1081 ttgtagtgtt catcaacaat ttaatattga tgatatagat ttttatagag aaaaatatcc
     1141 aggtattatt gtagctgttc atccagagtg tgatccatct gtttgcgata aagctgattt
     1201 tgttggttca acttcacaat taataaaatt tataaaagag ctaccagagg atcaaaaagt
     1261 tgttgttgga acagagttta atatggttaa tagattaaga gagaaaaata catatatttt
     1321 aagctcaaca aaaccagagt gccctacgat gaatgagaca actttagagc atgtttattt
     1381 aactttaaaa tctataaaag agaataaaat aagcgaatta tcagagataa aagtagatga
     1441 aaagacaaga tattgggcaa aaatagctct tgaaaggatg tttgaaatat gattcatatt
     1501 aaaaggtttg taaagcaagc aataacagaa gacaacggaa gaggagattt attctacgat
     1561 attgctcctg atggaaaatt tacagcaaaa attgtttcaa aaagtaatgg gatattagct
     1621 ggtgttaaat atgctgaaac tttggctcaa acagagaaga taaaagttga ttttctaaaa
     1681 aaagatggtg ataaaataca agctggagat attttagcaa aactagaagg aaaagcttca
     1741 aaactacttt caagcgagag aacatttttg aatatgcttc aacacgcaag tggaatagct
     1801 actatggcta gcaaatttgt tgaaaaacta gatggttttg atgttgtttt attagatact
     1861 agaaaaacac gaccacattt gagagatttt gaaaaatatg cttcaagagt tggtggagct
     1921 ataaatcata ggttgggtct tgatgattgt ttgatgctta aagatactca ccttagaact
     1981 attgaaaatc tagcagaatt tgtaaaaata gcaagaaaaa gaatatcttg ggttacaaaa
     2041 atagaaattg agtgtgaaac tttttctcaa gttgaaacag ctatggaagc tggagctgat
     2101 attataatgt gtgataatat gacattccaa caaataaaag aagttgtaaa atatagagat
     2161 caaaaatacc ctcatatttt aatagaagct agtggaaata taaatcttga tactattcaa
     2221 gaatatgcaa aaactggtgt tgatgctgtt agtagtggaa gtataattca tcaagcaact
     2281 tggcttgact tttcaatgag agttgattga ttttgaaaaa agattttatt tattttgtta
     2341 agaaaataat tttgtattgc atga
//
LOCUS       DAOQAN010000138         2359 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_65451_length_2359_cov_2.330295, whole genome shotgun sequence.
ACCESSION   DAOQAN010000138 DAOQAN010000000
VERSION     DAOQAN010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65451_length_2359_cov_2.330295"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /locus_tag="PKO10_03025"
     CDS             <1..723
                     /locus_tag="PKO10_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012011963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HNW66154.1"
                     /translation="DLGMIGKEDIVLAISYSGESEELIQILPHIKRFNIPLIAMAKDE
                     KSTLAKYSDYFININVQKEACPLDTAPTSSTTLTMAMGDALAVCLMKRRDFKKEDFAS
                     FHPGGSLGKKLFVKVDDLLRKDNLPIVSRETKLKDAIIVMSEGRLGSVIIEDENKKVI
                     GVLSDGDLRRALMKEGFSIDCNVEAIATLNPRTFDDENLLASDALQIIETYKIQLLII
                     TNKNRELKGLLHIHDLIEAGIK"
     gene            720..1568
                     /locus_tag="PKO10_03030"
     CDS             720..1568
                     /locus_tag="PKO10_03030"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014472858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNW66155.1"
                     /translation="MKNETRKEQKKDTKKPEVRTRAVEELRLNKFISHNSNYSRREAD
                     ALIAEGKVKVNNRVITDLATKVKVSDKVEIGKKIIKEDKERMYTVIVYNKPKGELVTK
                     SDPQGRKTIYDGLDSKYKHFNSVGRLDYASEGVLLLSDSVNVVNALMHSNLERVYKVK
                     VSGPISPKIEMAMQKGLEIDDATKGAYKKTKIKSMNFAPFLAYDIQTNGEKISKIKVV
                     ISEGKNRELRRFFGYFGLDVMDLKRVEYGGVSLNNLPTGKTRFLTKDEYKNLRIFLNE
                     EDDRLS"
     gene            1552..>2359
                     /locus_tag="PKO10_03035"
     CDS             1552..>2359
                     /locus_tag="PKO10_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012011961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNW66156.1"
                     /translation="MIDLANKYRPTSLDSFVGQSHIISKEKALYKLIKQKDIPHLFFY
                     GKPGTGKTTLAKIIAREISSDYHYFNATTFKIEDLRKVFDRYKNSFIKPLIFIDEVHR
                     LSKNQQEVLLPIMENYDATIIGASTENPFFTLTNAIRSRSFLYEFLPFSYDEMEKILN
                     IVLKDIDITLSNEVKDYLIYSSSGDARAMLTLLNFSYKVDQEISLNSLKELRANVIGD
                     GVSSSSSHYDLASAMIKSLRGSNVDAALYYMARLIDGGESVDFITRRFVIF"
BASE COUNT          942 a          252 c          430 g          735 t
ORIGIN      
        1 gatttgggaa tgattggaaa agaggatata gttttggcta tttcatatag tggagagagc
       61 gaggagctta ttcaaattct tccacatata aaaagattta atattccttt gattgcaatg
      121 gcaaaagatg aaaaatctac tttagcaaaa tattcagact attttatcaa tattaacgta
      181 caaaaagagg cttgtcctct tgatactgct ccaacatcat ctactacgct aactatggca
      241 atgggtgatg cacttgctgt atgccttatg aaaagaagag attttaaaaa agaggatttt
      301 gcatcttttc atcctggtgg aagtttaggt aaaaagctat tcgtaaaagt agatgacctt
      361 ttaagaaaag ataatcttcc tattgtttca cgtgaaacaa aattaaaaga tgcaataatt
      421 gtgatgagtg aaggaagact aggttctgta attattgaag atgaaaataa aaaagttatt
      481 ggtgttttaa gtgatggaga tttaagaaga gctttgatga aagaaggttt ttctattgat
      541 tgcaatgttg aagctattgc aactttaaat ccaagaacat ttgatgatga gaaccttttg
      601 gcaagtgatg ctttacagat tattgagact tataaaatac aacttcttat aattactaat
      661 aaaaatagag agcttaaagg tttattacat attcacgacc taatagaggc aggtataaaa
      721 tgaaaaatga aacaagaaaa gaacaaaaaa aagatacaaa aaaaccagaa gtaagaacaa
      781 gagcagttga agagttaaga ttaaataaat ttatatctca caacagtaat tactcaagaa
      841 gagaagcaga tgctttaata gctgaaggaa aagtaaaggt taataataga gttattacag
      901 atttagctac aaaagttaaa gtaagtgata aggttgaaat tggtaaaaag attatcaaag
      961 aagacaaaga gagaatgtac acggtaatag tttataataa accaaaaggt gagctagtaa
     1021 caaaaagtga ccctcaaggt agaaagacta tttatgatgg tttagatagc aaatataagc
     1081 actttaatag tgtagggcga cttgactatg caagtgaagg agttttgctt ttaagcgata
     1141 gtgtaaacgt agtaaatgct ttaatgcatt caaatcttga aagagtttat aaggtaaaag
     1201 taagtggtcc aatatctcca aaaattgaga tggctatgca aaaaggtcta gagatagatg
     1261 atgcaacaaa aggtgcatat aaaaagacaa agataaaatc aatgaatttt gctccatttt
     1321 tagcatatga cattcaaaca aatggagaaa aaatttctaa gataaaagta gtaattagtg
     1381 aaggtaaaaa tagagaacta agaagatttt ttggatattt tggacttgat gttatggatt
     1441 taaaaagagt tgaatatggt ggggttagtt taaataatct accaactgga aaaacaagat
     1501 tccttacaaa agatgagtat aaaaatttac gaatcttttt aaatgaagaa gatgatagac
     1561 ttagctaaca aatatagacc aacatcactc gatagttttg ttggtcaatc tcatattata
     1621 agtaaagaga aagctcttta taaattgata aaacaaaaag atattcctca tctatttttt
     1681 tatggaaaac ctggaactgg aaaaactact ttagcaaaaa ttatagctcg tgagataagt
     1741 agcgattatc actattttaa cgcaacaaca tttaaaattg aagatttaag aaaggtattt
     1801 gatagatata aaaatagctt tattaaacct ttaatattta ttgatgaagt tcatagactt
     1861 tcaaaaaatc aacaagaagt tctacttcca attatggaaa attatgatgc tacaataatt
     1921 ggagcaagta cagaaaatcc attttttact cttacaaatg caataagatc aagatcattt
     1981 ttatatgaat ttttaccatt ttcttatgat gaaatggaaa aaatattaaa tatagtttta
     2041 aaagatattg atataacttt atcaaatgaa gttaaagatt acttgattta ttcaagttct
     2101 ggtgatgcta gagcgatgct tactctatta aatttttcat ataaagtaga tcaagagatt
     2161 agtttgaata gtttaaaaga gttaagagca aatgttatag gtgatggggt tagttctagt
     2221 agttctcact atgatttagc aagtgcaatg attaaatctt taagaggttc aaatgttgat
     2281 gctgcattat actatatggc aagattaata gatggtggag agagtgttga ttttattacg
     2341 agaagatttg taatatttg
//
LOCUS       DAOQAN010000139         2358 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_65491_length_2358_cov_2.294833, whole genome shotgun sequence.
ACCESSION   DAOQAN010000139 DAOQAN010000000
VERSION     DAOQAN010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65491_length_2358_cov_2.294833"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            63..890
                     /locus_tag="PKO10_03040"
     CDS             63..890
                     /locus_tag="PKO10_03040"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013136003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HNW66157.1"
                     /translation="MEIEKNSSTYNQIEKAIKYIDENFKSHPSIDEIAKNVGMSKYHF
                     IRVFKDYVGLTPQQFLHSVTLNYAKEHIKESKSILDSSLDIGLSSSSRLHELFVNLIG
                     VTPKEWKEKGKDVEITYGFGQTPFGEALIGFTSKGICYLGFCDNNKKDIFQRFNELWE
                     NANLVFNEKLANEYLENIFIKNKKYPLFVKGTNLQINVWKALINIPNGKIATYSDIAN
                     IVDKPKSVRAVASAIGKNHIGYLIPCHRVIAKSGAMSGYRWGIERKENLLAFEASKK"
     gene            977..>2358
                     /locus_tag="PKO10_03045"
     CDS             977..>2358
                     /locus_tag="PKO10_03045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HNW66158.1"
                     /translation="MQLLSKDAPLEISLTKMKKIIDDLGYKIRLSKEKNPVKNSFSVN
                     LSFEGANNYIFSNGKGSLSQSSLASAYGEFIERLQTNLYFNDYYLENRDFFPDQKGFD
                     KNSDFLDEDIKKYYTLEGIEKDDFLDFNSNNFSQIISLPFINQTSQKKVYFPINLLAN
                     LYASNGLSTGNTKDEAKVQALCEIFERNVKIKVIKDGLSLPEFPKEILNNFSKITEDI
                     KSLEEKGFKIKVYDSSLGGIYPVTAIAFINPKNNTLFLSFGSHPILEVSIERTLTELL
                     QGREIDELDSFEKPTFDMEDIASNSNLESHFVDSNAKVGLQFLSSKKSFAYTPWKFDT
                     EKSSEQFAILKEILNKENKTIYLREYDYLGFYSCHMIVPDFSEIYPLEDMIYNNKNQG
                     KLIRDFILNKDKYDLENLLDELSGFDDSINMGAFIGVIFKEDFSLGEFKADILIKLKE
                     YEEAIFCLEQS"
BASE COUNT          948 a          266 c          369 g          775 t
ORIGIN      
        1 tgaagagtta gctagtaagt ttgtgattta cagtagttcg acgtattaat aaagagaaaa
       61 aagtggaaat agaaaaaaat agttctactt ataatcaaat agaaaaagcc atcaaataca
      121 tagatgagaa ctttaaatct catccaagta ttgatgagat tgcaaaaaat gttggaatga
      181 gtaagtacca ttttataagg gtttttaaag attatgttgg tcttactcct caacagtttt
      241 tgcacagtgt tactttaaac tatgcaaaag agcatataaa agagtccaaa tctattcttg
      301 atagtagttt agatattgga ctatcaagtt ccagccgtct tcatgaactt tttgtaaact
      361 taattggagt tactccaaaa gagtggaaag aaaaaggtaa agatgtagag ataacttatg
      421 gttttggaca aactcctttt ggtgaggctt tgattggatt tacatctaaa ggaatatgct
      481 atttaggttt ttgtgataac aataaaaaag atatttttca aagatttaat gaactttggg
      541 aaaatgccaa cttagttttt aatgaaaaat tagcaaatga gtatttggaa aatatcttta
      601 taaaaaataa aaagtatccc ctttttgtaa aaggtacaaa tcttcaaata aatgtttgga
      661 aagcattaat aaatattcca aatggaaaaa ttgcaactta tagcgatatt gcaaatattg
      721 tagataaacc aaaatctgta agagcggttg caagtgctat tggaaaaaat catataggat
      781 atttaattcc ttgccacaga gtaatagcaa aaagtggtgc tatgagtggc tatagatggg
      841 gaattgaaag aaaagagaat ttactagctt ttgaagcctc gaaaaaataa aataaaaatc
      901 aataaaatat tcaaataagt gcttaaaaaa gataatcttt ggctatactt cacaaaattt
      961 ttaaaaaaga agtaatatgc aactactgtc aaaagacgcc ccactagaaa taagtttaac
     1021 aaaaatgaaa aaaatcatag atgatttagg ttacaaaata aggctttcaa aagagaaaaa
     1081 tcctgtaaaa aatagttttt cagtaaactt aagttttgaa ggtgcaaaca actatatatt
     1141 ttcaaatggt aaaggttcac tatcacagtc atctttagca agtgcttatg gtgaatttat
     1201 agaaagatta caaacaaatc tatattttaa tgattactat ttagaaaata gagatttttt
     1261 tccagaccaa aaaggatttg ataaaaatag cgatttttta gatgaagata taaaaaaata
     1321 ttacacttta gaaggtattg aaaaagatga ttttttggat tttaactcaa ataatttttc
     1381 acaaataatc tctttacctt ttataaatca aacttcacaa aaaaaagtat attttcctat
     1441 aaatttacta gcaaatcttt atgcaagtaa tggattatct acaggaaata caaaagatga
     1501 agcaaaagtt caagctctat gtgagatttt tgaaagaaat gtaaaaataa aagttataaa
     1561 agatggttta tctcttccag agtttccaaa agaaattcta aataattttt caaaaataac
     1621 tgaagatatt aaatctttag aagaaaaagg atttaaaata aaagtttatg actcatcttt
     1681 gggcggaata tatccagtta cagcaatagc ttttataaat ccaaaaaata atacactttt
     1741 tttatctttt ggaagccacc ctattttgga agtttcaatt gaaagaacac ttacagaact
     1801 tttacaagga agagaaatag atgagctaga tagctttgaa aaaccaactt ttgatatgga
     1861 agatattgct tcaaactcaa atctagagtc tcattttgtt gattcaaatg caaaagttgg
     1921 cttacagttt ttaagttcta aaaaatcatt tgcttatact ccttggaagt ttgatacaga
     1981 aaaaagtagt gaacaatttg caattttaaa agagatttta aataaagaaa ataaaaccat
     2041 atatcttaga gaatatgact atttaggttt ttactcttgt catatgattg ttccagattt
     2101 ttcagagatt tatccacttg aagatatgat ttataacaat aaaaatcaag gaaaactaat
     2161 ccgtgatttt attttaaaca aagataaata tgatttagaa aatttacttg atgagttaag
     2221 tggttttgat gactctataa atatgggcgc atttattgga gttattttta aagaggattt
     2281 ctctttgggt gaattcaaag ctgatatttt gattaaatta aaagagtatg aagaggcaat
     2341 tttttgtctt gaacaatc
//
LOCUS       DAOQAN010000140         2356 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_65561_length_2356_cov_3.462842, whole genome shotgun sequence.
ACCESSION   DAOQAN010000140 DAOQAN010000000
VERSION     DAOQAN010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65561_length_2356_cov_3.462842"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1898)
                     /locus_tag="PKO10_03050"
     CDS             complement(<1..1898)
                     /locus_tag="PKO10_03050"
                     /inference="COORDINATES: protein motif:HMM:NF019935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNW66159.1"
                     /translation="MIKSFDIKNFKDKSSFEVSKIFKKNYIDYKNSANTSNSKLFFKQ
                     NTKNIDKFLFQIFSYLSLNSFKHNSFVSFVALGSYARVELCLHSDIDVMILYKNSKSY
                     DILKIIEKFVAFAWDCGLNLGLRVVNIDNIDEMYDLAKNDITIKTSLLESRYIFGSKK
                     IYEDFELFVKNLRQIDKKAFVQEQLTTHTQRLQKYPLNMQVNLKDGYGGIREANKLFW
                     ILNVVYDVQKAKELVGTKLNYDSYKKFTIALDFLFQTRNALHTIHKKKVDTITFDTLP
                     EVSSKLNFKNEFEFMAKLFSSLHIIHNFHSNMSSKFIRTMFFDTSNLEVLRKSRFKKG
                     IYILDNKIYCSFHRSFVDLKTLLKELISFPQTIKGFDRSYIYFASRVKIPKIINKSLK
                     KDIKTLLTNENLYPILKLFYNSELINRLIPSFKKISNLAQFGGYHKHPVDIHTLQTVK
                     YSMYIEDEFIKSVFNSLCKEEQILTRLIALFHDIGKGRKKDHHIIGENIFKKTMKNFG
                     FDDEFIKMGANIIRYHDQMSRTATNEDIYSQKAILNFIGLFKNINELKILYVVTYCDI
                     CAVNKPLYNSSISMLLKELYKKSILAFENPELIKESITRVSKLNKMKTLEKYQELPNI
                     LKRKIAQIS"
     gene            complement(1895..>2356)
                     /locus_tag="PKO10_03055"
     CDS             complement(1895..>2356)
                     /locus_tag="PKO10_03055"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNW66160.1"
                     /translation="KNDRGITALEFAILNDKDDILKLLLDLRANTNFVDEKGRNLIHT
                     TIFKDKEKYIKIISQYNNTIINQADSFGAKPINYAAFMGKKSVVLELIDLGASINNAN
                     KKSPNILEFLKRYHKNILNLSSGVDDSNNKSNLNKLAENMILEFEIDISKQ"
BASE COUNT          795 a          305 c          267 g          989 t
ORIGIN      
        1 gagatttggg ctatttttct ttttaaaata tttggtaact cttgatattt ttctaaagtt
       61 ttcattttat taagttttga aactctagta atactctctt ttataagttc tggattttca
      121 aaagctaaga tacttttttt gtaaagttct tttaaaagca ttgaaattga gctattgtaa
      181 agaggtttat taactgcaca aatatcacaa taagttacaa catacaaaat ctttagttca
      241 tttatatttt tgaataatcc tataaagttc aaaatagctt tttgtgagta gatatcttcg
      301 tttgttgcag ttcttgacat ttggtcgtga taccttataa tatttgcacc catttttata
      361 aattcatcat caaaaccaaa atttttcata gtttttttga aaatattttc tccaataata
      421 tgatggtctt ttttccttcc ttttccaata tcatgaaaaa gtgcaataag tcttgttaaa
      481 atctgctcct ctttacaaag agagttaaaa acagatttaa taaattcatc ttctatatac
      541 atactatatt ttactgtttg tagtgtgtgg atatctactg gatgtttatg ataaccacca
      601 aattgagcta aatttgatat ttttttgaag gagggaatta atctatttat tagttcacta
      661 ttataaaata gttttaaaat agggtacaaa ttctcatttg taagaagtgt tttaatatct
      721 ttttttagtg atttatttat gatttttggg attttaactc tacttgcaaa gtatatgtaa
      781 gacctatcaa aaccttttat tgtttgagga aatgatatta gctcttttaa aagagttttt
      841 aaatcaacaa aacttctatg aaaagagcaa tatatcttat tgtctaaaat atagatacct
      901 tttttaaatc ttgattttct caaaacttct aaatttgaag tatcaaaaaa catagttctt
      961 ataaattttg aactcatatt tgagtgaaaa ttatgaatta tatgaaggct tgaaaatagt
     1021 tttgccataa attcaaactc atttttaaag tttaatttac tacttacttc aggaagtgtg
     1081 tcaaaagtaa ttgtatctac ctttttttta tgaatagtat gaagagcgtt tcttgtttga
     1141 aataagaaat ctaaagcaat tgtaaacttt ttataagaat cataatttag tttcgtacct
     1201 acaagttctt ttgctttttg aacatcatat acaacattta aaatccaaaa aagcttattt
     1261 gcttctctta ttccaccata tccatctttt aagtttactt gcatatttag aggatatttt
     1321 tgtaatcttt gagtgtgagt agttagctgt tcttgtacaa aagctttttt atctatttgt
     1381 cttagatttt taacaaacaa ttcaaaatct tcataaatct tctttgaacc aaaaatatat
     1441 cttgattcaa gaagtgaagt tttaatagta atatcatttt ttgctaaatc atacatttca
     1501 tcaatattat caatattcac aactcttaat ccaagattta atccacaatc ccaagcaaag
     1561 gctacaaatt tttctattat tttcaaaata tcataagatt tactattttt gtataaaatc
     1621 ataacatcaa tatcagagtg taaacataac tctactcttg cataagaacc taatgcaaca
     1681 aagctaacaa atgagttatg tttaaaacta ttcagactta aataagagaa aatttgaaat
     1741 aagaatttat ctatattttt tgtattttgt ttgaaaaaga gtttactatt gcttgtattt
     1801 gcactatttt tataatctat ataatttttt ttaaatattt ttgatacttc aaaactactt
     1861 ttatctttaa agttttttat atcaaaagat ttaatcattg ttttgaaata tctatttcaa
     1921 actctaaaat catattttca gctagtttat ttaggtttga tttgttgttt gaatcatcaa
     1981 cgccagagct taaatttaaa atattcttgt gatacctttt tagaaattcc aatatatttg
     2041 gtgatttttt atttgcatta tttattgatg ctcctaaatc aataagttct aaaacaacac
     2101 tttttttacc cataaaagct gcataattta taggttttgc cccaaaacta tctgcttgat
     2161 ttataatagt attgttgtat tgagatatga ttttaatata tttttctttg tctttaaaaa
     2221 ttgttgtatg gatgaggttt cttccttttt catctacgaa atttgtgttt gctcttaaat
     2281 ccaaaagaag ctttaaaata tcatctttat catttaaaat cgcaaactct aaagctgtaa
     2341 tacctctatc attttt
//
LOCUS       DAOQAN010000141         2352 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_65721_length_2352_cov_2.877667, whole genome shotgun sequence.
ACCESSION   DAOQAN010000141 DAOQAN010000000
VERSION     DAOQAN010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65721_length_2352_cov_2.877667"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1269)
                     /gene="soxC"
                     /locus_tag="PKO10_03060"
     CDS             complement(<1..1269)
                     /gene="soxC"
                     /locus_tag="PKO10_03060"
                     /EC_number="1.8.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite dehydrogenase"
                     /protein_id="HNW66161.1"
                     /translation="MKNSEVLEIEKSSLSLETTSRRDFFKKTAINLAGAISAATVLSP
                     MSLKADDKAIIKEAPWGQKLGDPVDKNLYGIPSPYEHNNIRRTHNLLSSGDAYASISL
                     CPIHESEGIITPNGLFFTRNHGGTAHIDPNEFRLMIHGKVKREIVLTLEDLKRYPSET
                     RTYFIECPANGSPEWRGPQFNSLQFMKGMMSSAQWTGVMLKTILDDIGLEKDAVWMLA
                     VGSDNASNPRTIPVEKALDDVMVVWAQNGEALRPEQGYPVRLVVPGWEGNLNTKWLNR
                     LEFSDKPWHAKEETSKYTMLQKDGRSVRFFWVNEVNSVITKPCPEKPWTHLKAGDMVE
                     IEGLAWSGHGTIKGVDISFDGGDNWVEAKLKGLVLPKSWTRFSYIYKWDGKPLFLSSR
                     AYDDFGNVQPTIDEETKAVGVESVYHRNAIV"
     gene            complement(1462..>2352)
                     /locus_tag="PKO10_03065"
     CDS             complement(1462..>2352)
                     /locus_tag="PKO10_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014473399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNW66162.1"
                     /translation="GAVCWFFNLGLTYFVALPIFSLGMLMMLIKEYGKEEPWLNLALS
                     IGVVGEIVSILALTLFTSWTENSGLSSGFFISILTIISVIIGTILLLRFSYMLFWWFP
                     EVKKYLIPDNIDDKHNQDIRFSISLLLILVSIMLILKIDVVLGAFTAGLFFKMFFMQR
                     EELLHKIESFGFGFFAPIFFIYTGSTVKLDMITLEILKHAIFIMGTIISIRLISSYLV
                     FLNYLKPKQTTLFALSDSMPLTFMVAIAMISYNYGLITQEEYFSFIIASMLDGLILMI
                     VIRKLYKLFKLDNKTIENKT"
BASE COUNT          829 a          414 c          273 g          836 t
ORIGIN      
        1 tacaattgca tttctatgat aaacactctc tacaccaaca gcttttgtct cttcatcaat
       61 cgttggttga acatttccaa agtcatcgta agctcgactt gataaaaata gtggctttcc
      121 atcccattta taaatataac taaatctagt ccatgatttt ggtaaaacca aaccttttag
      181 ttttgcttca acccagttgt ctccaccatc aaaagatata tcaacacctt taattgttcc
      241 atgaccactc caagcaagac cttcaatttc caccatatca ccagctttta gatgcgtcca
      301 tggcttttca ggacaaggtt ttgtaattac agagtttact tcatttaccc aaaaaaatct
      361 tacagatctt ccatcttttt gaagcattgt gtattttgaa gtttcctctt ttgcatgcca
      421 aggtttatca ctaaattcaa gtctatttaa ccatttagta tttaaatttc cttcccaacc
      481 tggaacaaca agtcttactg gatatccttg ctcaggtctt aatgcttcac cattttgagc
      541 ccatactacc ataacatcat caagtgcttt ttcaacagga atagttctag gatttgaggc
      601 attatcactt ccaactgcta acatccaaac ggcatctttt tctaaaccaa tatcatcaag
      661 tatagttttt agcataactc cagtccattg agcactactc atcataccct tcataaattg
      721 taaactatta aattgtggac ctctccattc tgggcttcca tttgcaggac attcaataaa
      781 ataagttcta gtttcacttg ggtatctttt taaatcttct aaagttaaaa caatttctct
      841 ttttacttta ccatgaatca ttaatctaaa ctcatttgga tcaatatgtg cagttcctcc
      901 gtgatttctt gtgaaaaata gtccatttgg agtaattatt ccttcacttt catgaattgg
      961 gcacaaagat atagaagcat aagcatctcc agaagataga aggttgtgag ttcttctaat
     1021 attgttatgc tcataaggtg aaggaatacc atatagattt ttatctacag gatcacctag
     1081 cttttgtccc catggggctt ctttgattat tgctttatca tcagctttta aagacatagg
     1141 tgataaaaca gttgcagcac ttattgctcc agctaaattg atagcagttt ttttaaaaaa
     1201 atctcttcta cttgtagttt ctaaagataa agagcttttt tctatttcta aaacttcact
     1261 atttttcaag ctaacctcct aataaaattt aatattattt caaataattc tgaattttaa
     1321 ttataggttt tataagttga aatgaaactt aaaatattaa tttttcttat ataataattt
     1381 ttaaaaatac gaaaaaatag cacatacata gcatcaataa aaatatatta tattttaaat
     1441 ttgttttatt tataaaacta tttaagtttt attttctatt gttttattat ctaatttaaa
     1501 taacttatat aattttctaa ttactatcat taaaatcaag ccatctaaca tacttgcaat
     1561 aataaaagag aaatactctt cttgagttat taatccataa ttgtatgaaa tcattgcaat
     1621 tgcaaccata aaagtaagtg gcattgaatc acttagtgca aaaagtgttg tttgttttgg
     1681 ttttaaatag tttaaaaaaa ctagatatga actaattaat cttattgaaa ttatagttcc
     1741 cataataaat atagcatgtt ttagaatttc taaagttatc atatctaact ttactgttga
     1801 gcctgtgtag ataaagaaaa taggtgcaaa aaaaccaaag ccaaaagatt cgattttatg
     1861 taataactcc tctctttgca taaaaaacat tttaaagaaa agaccagctg taaatgctcc
     1921 taaaacaaca tctattttta aaataagcat aattgatact aaaattagca ataaagatat
     1981 tgaaaatctt atgtcctgat tgtgtttatc atctatatta tctggaatta aatatttttt
     2041 aacttcagga aaccaccaaa aaagcatata agagaatcta agaagtaaaa tcgtaccaat
     2101 tattacacta ataattgtta aaattgatat aaaaaatcca ctacttaaac cactattctc
     2161 agtccaacta gtaaaaagtg taagtgctaa aatacttact atttcaccaa caactccaat
     2221 agaaagagct aaatttaacc acggttcttc ttttccatac tcttttatta gcatcattaa
     2281 cattcctaat gaaaagatag gaagcgctac aaaatatgtt aaacctaaat taaaaaacca
     2341 acaaacagct cc
//
LOCUS       DAOQAN010000142         2348 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_65901_length_2348_cov_3.587876, whole genome shotgun sequence.
ACCESSION   DAOQAN010000142 DAOQAN010000000
VERSION     DAOQAN010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65901_length_2348_cov_3.587876"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..509)
                     /locus_tag="PKO10_03070"
     CDS             complement(54..509)
                     /locus_tag="PKO10_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HNW66163.1"
                     /translation="MKINIYSIVKPSNDDFDKLIKEFIKMSSKYAKVEIFYIFNKDIA
                     KAQTIGENESKKIYTKSYLPYLKGYNIALDVLGKSVDSFAFSKLLEDKNEVNFFIGGA
                     FGFENEFLSLCDSVISLSNLTFAHKIATLILSEQIFRSLSIINNHPYHK"
     gene            complement(499..1110)
                     /locus_tag="PKO10_03075"
     CDS             complement(499..1110)
                     /locus_tag="PKO10_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HNW66164.1"
                     /translation="MSSNLEKALGFNLEAFNYLYVLCDYETLLKKNITLENFLEILKK
                     YDVKLLQYRDKVSDLELQKQNLIFLKQNLNIPIIVNDKVELIDYADGLHVGQDDILKI
                     HKDKKLAIKLLRKRVKNKLLGLSTHNELEILEANNFELDMIGLGAYKVTNTKDISNIL
                     GEKLQYLAKMSKHPVCAIGGVKVDDIIKNTKFNCVGSAILNEN"
     gene            complement(1110..1976)
                     /gene="accD"
                     /locus_tag="PKO10_03080"
     CDS             complement(1110..1976)
                     /gene="accD"
                     /locus_tag="PKO10_03080"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HNW66165.1"
                     /translation="MDLKNLFSKISFDNKNKEQATKKDAPSHWIKCPSCSSLMFFKEV
                     ENQDNVCPKCEFHMRIGAKRRVEILADENSFVEFDTNLKPNDPLKFVDKLSYKKRVEE
                     GLAKTGRVSSVISGECTINSIGVQLVVFDFAFMGGSLGSVEGEKIVRAVNRAIEKKQA
                     VIIVSASGGARMQESTFSLMQMAKTSAALKKLDSYKLPYISILTDPTMGGVSASFAFL
                     GDIIMAEPGALIGFAGQRVIKQTIGTDLPEGFQRAEFLLEKGSIDMVVNRRDMKKTLT
                     DLLTIFGQEKIS"
     gene            complement(2023..>2348)
                     /locus_tag="PKO10_03085"
     CDS             complement(2023..>2348)
                     /locus_tag="PKO10_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002880668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine adenosyltransferase domain-containing
                     protein"
                     /protein_id="HNW66166.1"
                     /translation="RWIAKHIVASGLAKKAIVQISYAIGVARPTSVAVDTMGTYTKHN
                     DDILSAFVMENFPLTPRWITEKFNLDKPSATTFLYADVAARGQVGQPDYPWEKLDELD
                     KFKNI"
BASE COUNT          770 a          392 c          275 g          911 t
ORIGIN      
        1 tttttccttt ttgaagagtt aattgttttt tattatacaa aaaagtttat aaattactta
       61 tgatatggat gattatttat aatacttaaa gatctaaata tctgttctga tagtatcaga
      121 gtggcaattt tatgggcaaa tgtaagatta cttagggaaa ttacactatc acaaagagat
      181 aaaaactcat tttcaaaacc aaaagctcca cctatgaaaa aatttacctc atttttatct
      241 tccagaagct tagaaaaagc aaaactatcg acacttttac ctaaaacatc aagagctata
      301 ttgtaacctt tcaaatatgg taaataactt tttgtatata ttttttttga ttcattttct
      361 ccgatagttt gtgcttttgc tatatcttta ttaaatatat aaaaaatttc aacttttgca
      421 tatttactag acattttaat aaactcttta ataagtttat caaaatcatc attacttggt
      481 tttactattg agtaaatgtt aattttcatt taaaatagca cttccaacgc aattaaattt
      541 tgtatttttg ataatatcat caacttttac accaccaata gcacaaacag gatgtttaga
      601 catttttgcc aaatattgta gtttttcacc caaaatattt gaaatatctt ttgtatttgt
      661 aactttgtaa gcacctaaac caatcatatc aagctcaaaa ttatttgctt ctagaatttc
      721 tagttcattg tgagttgaga gtcctaaaag tttatttttt actctttttc gtaaaagttt
      781 tatagctaat tttttatctt tgtgtatctt taaaatatca tcttgaccta catgcaaacc
      841 atctgcatag tcaattaatt ctactttatc atttacaata ataggaatat ttagattttg
      901 tttaagaaaa ataagatttt gtttttgtaa ctctaaatct gaaactttgt ctctatactg
      961 taaaagtttt acatcatatt tttttaaaat ttccaaaaaa ttttcaagag ttatattttt
     1021 ttttaaaaga gtttcgtaat cacacaaaac ataaagatag ttaaaagctt caagattaaa
     1081 tcccaaagct ttttctaaat ttgaagacat tagctaattt tttcttgtcc aaaaatagtt
     1141 aataaatctg taagagtttt tttcatatct cttctattta caaccatatc aatagaacct
     1201 ttttctagta aaaattcagc tctttggaaa ccttcaggaa gatcagttcc aattgtttgc
     1261 ttaattactc tttgtccagc aaaaccgatt aaagcacctg gttctgccat aataatatca
     1321 cctagaaatg caaaagaagc agaaactcca cccatagttg gatctgttaa tatagagata
     1381 tatggaagtt tatagctatc gagttttttc aaagctgcag atgtttttgc catttgcatc
     1441 aaagaaaaag ttgattcttg cattcttgca ccacctgatg ctgatactat aataactgct
     1501 tgtttttttt caattgctcg attaactgct cttactattt tttcaccctc aacactacct
     1561 aaacttccac ccataaatgc aaaatcaaaa actacaagct gaacaccaat tgagttaatt
     1621 gtgcactcac cactaataac acttgatact cttcctgttt ttgcaagtcc ttcttcaact
     1681 ctttttttat aagagagttt atctacaaac ttcaaagggt catttggttt taaatttgta
     1741 tcaaactcta caaagctatt ttcatctgca aggatttcta ctcttctttt tgcccctatt
     1801 ctcatatgaa attcacattt tgggcaaaca ttatcttgat tttcaacctc tttgaaaaac
     1861 atcaaagagc tacaactagg acattttatc cagtgacttg gagcatcttt ctttgtagct
     1921 tgctctttat ttttgttatc aaatgaaatt ttgctaaata gattttttaa atccactacc
     1981 ttttctcctt tttttctaaa gcaatattat agttaaatct aactaaatat ttttaaattt
     2041 atctagttcg tctaattttt cccaaggata atcaggttgt ccaacttgac cacgagctgc
     2101 tacatctgca taaaggaatg ttgtagcact tggtttatct agattaaatt tttctgtaat
     2161 ccatcttggt gttaaaggga aattttccat tacaaatgca gataaaatat catcattatg
     2221 ttttgtatat gttcccattg tatcaacagc aactgatgtt ggacgtgcca ctccaatagc
     2281 ataagatatt tgtacaatag ctttttttgc aagtcctgat gcaactatat gtttagcaat
     2341 ccatcttg
//
LOCUS       DAOQAN010000143         2342 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_66202_length_2342_cov_2.043725, whole genome shotgun sequence.
ACCESSION   DAOQAN010000143 DAOQAN010000000
VERSION     DAOQAN010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66202_length_2342_cov_2.043725"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="PKO10_03090"
     CDS             <1..308
                     /locus_tag="PKO10_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HNW66167.1"
                     /translation="SIYLGLEGGKGVATGLGVYIVLIPISTLIGAIVWFICAKVLKIS
                     SLSSLLALIAVIISSIFINDGLNIGSNAPMYLIAFIIIYKHIPNIIRLLKGEEKKVI"
     gene            305..616
                     /locus_tag="PKO10_03095"
     CDS             305..616
                     /locus_tag="PKO10_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HNW66168.1"
                     /translation="MKIEIEQLTFKCIIGILDFERVKKQKVIINLSFDYDFSKDNFID
                     YSEVANLVKKSMKKNKFKLLEDAVKTIKKVLEDSYTIKNLKLKISKPNILKDCIVSIS
                     S"
     gene            672..1286
                     /locus_tag="PKO10_03100"
     CDS             672..1286
                     /locus_tag="PKO10_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014469358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66169.1"
                     /translation="MKKIIASCALLCGASLLADTTMCFKENHPSMATIETVPLNGGAC
                     GGKYSINDMKKKGWLVDDIKINGSSYIYILKTQDHGKTMAGVAPSGISQEQMEANILA
                     KLEAKQEAEEQAAIEKANETMLADAKVLYINQCQSCHGEKGDKTKGNSKLKNISQKDM
                     EQALKDYELGISEEKRSSIYGPAHINFLNNSSIKGIKAYLDSIQ"
     gene            1380..>2342
                     /gene="prfB"
                     /locus_tag="PKO10_03105"
     CDS             1380..>2342
                     /gene="prfB"
                     /locus_tag="PKO10_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014475267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HNW66170.1"
                     /translation="MDAYEYSELLKLLNTKLNNIKGILKPDLLNQRLQEIIELESNQD
                     FWNDIENATKIGIEKNRILGKLNKFNKAYESLNGTNELYEMANSEKDEDTLELLYEEA
                     NDLEDLIKSTEISVMLSNPDDTLNAIVTIHPGAGGTESQDWASILYRMYLRWAERNDF
                     KVEVLDYQAGDEAGIKDVSFVIRGENAYGYMKTENGIHRLVRISPFDSNAKRHTSFSS
                     VMVSPEIDDNIDIVIEDKDIRIDTYRASGAGGQHVNKTESAIRITHIATNIVVQCQND
                     RSQHKNKASAMKMLKSRLYELELEKQRASKDTGDKSENGWGHQIR"
BASE COUNT          947 a          267 c          398 g          730 t
ORIGIN      
        1 actctatata tttagggctt gaaggaggaa aaggtgttgc aacaggactt ggagtttata
       61 ttgttttaat tccaatttct acactaattg gtgcaattgt ttggtttatt tgtgcaaaag
      121 ttttaaaaat atcatcttta tcttcacttt tggcattaat tgcagtaatt attagctcta
      181 tttttataaa cgatggctta aatataggtt cgaatgctcc tatgtatttg attgcattta
      241 ttattatata caaacatatt ccaaatatta taagactttt aaaaggtgaa gagaaaaaag
      301 tgatatgaaa atagaaattg aacaacttac ttttaaatgt attatcggaa ttcttgattt
      361 tgaaagagta aaaaaacaaa aagttataat caaccttagc tttgattatg attttagtaa
      421 agataatttt atagactatt cagaagttgc aaatcttgta aaaaaaagta tgaaaaagaa
      481 taaatttaaa cttctagaag atgcagtaaa aacaataaaa aaagttctag aagatagtta
      541 cacaattaaa aatctcaaat taaaaatctc aaaaccaaat attttaaaag attgtatagt
      601 aagtatctct tcttaaaaaa atttagtaaa attatggtaa aatcttgcat ttttaaaaaa
      661 aggattttat cttgaaaaaa attattgctt cttgcgcgct tttgtgtgga gcttctcttt
      721 tagctgacac tactatgtgt tttaaagaaa accacccatc tatggcaact attgaaactg
      781 ttcctctaaa tggtggagct tgtggaggaa aatactctat taatgatatg aaaaagaagg
      841 gttggcttgt tgatgatatt aaaataaatg gttctagtta tatttatatc ttaaaaactc
      901 aagatcatgg aaaaactatg gctggtgttg caccatctgg aattagccaa gaacaaatgg
      961 aagcaaatat tcttgctaag cttgaagcta aacaagaagc tgaagagcaa gctgctattg
     1021 aaaaagcaaa tgaaactatg ttagctgatg caaaagtttt atacataaat caatgtcaaa
     1081 gctgtcatgg agaaaaaggt gataaaacaa aaggtaattc aaaacttaaa aatataagcc
     1141 aaaaagatat ggaacaagct ctgaaagatt atgaattagg aattagtgaa gagaaaagaa
     1201 gttctattta tggacctgct catattaatt tcttaaataa ctcaagcata aaaggtataa
     1261 aagcttattt agatagtatt caataatttt aaagagctta ttgctcttta aatgatattt
     1321 tatttactca caacatattt taaaaacctc aaaatagaaa aactaaagga taaagctgta
     1381 tggatgccta tgaatattca gaacttctaa aactactaaa tacaaaacta aacaatataa
     1441 aagggatttt gaagcctgac ttactaaatc aaagattaca agagattatc gaacttgaat
     1501 caaatcaaga tttttggaat gatattgaga atgccacaaa aattggaata gaaaaaaata
     1561 gaattttagg aaaattaaat aaatttaata aagcttatga gtcattaaat ggtacaaatg
     1621 aactttatga gatggcaaat agtgaaaaag atgaagatac tttagaactt ttatatgaag
     1681 aagctaatga tttagaagat ttaataaaat caactgaaat atctgtaatg ctttcaaatc
     1741 cagatgatac actaaatgcg attgttacaa ttcatccagg agctggtgga acagagtctc
     1801 aagattgggc aagtatattg tatagaatgt atttaagatg ggctgaaaga aatgatttta
     1861 aagttgaagt acttgattat caagctggtg acgaagctgg aataaaagat gtaagttttg
     1921 taatacgtgg tgaaaacgct tatggttata tgaaaacaga aaatggaatt cataggttag
     1981 tgagaatctc tccatttgac tcaaatgcaa aaagacatac atctttctca tctgttatgg
     2041 tgagccctga aatagatgac aatattgata ttgtaataga agataaagat attagaattg
     2101 atacatatag agcaagtggt gctggtggtc agcacgtaaa caagactgaa agtgctatta
     2161 gaattactca tattgctaca aatattgttg ttcaatgtca aaatgataga agtcaacata
     2221 aaaataaagc aagtgctatg aaaatgctta aatcaagact ttatgaactt gagcttgaaa
     2281 aacaaagagc atcaaaagat actggtgata agagtgaaaa tggttgggga catcaaatta
     2341 ga
//
LOCUS       DAOQAN010000144         2331 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_66614_length_2331_cov_3.324253, whole genome shotgun sequence.
ACCESSION   DAOQAN010000144 DAOQAN010000000
VERSION     DAOQAN010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66614_length_2331_cov_3.324253"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            284..970
                     /gene="queC"
                     /locus_tag="PKO10_03110"
     CDS             284..970
                     /gene="queC"
                     /locus_tag="PKO10_03110"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014469352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HNW66171.1"
                     /translation="MNQNFNKKAVVILSGGMDSTLSSYIAKNSGYELIAIHFNYGQRT
                     QDRELKAFRDICKELNVLNKYEIDIPFFSQIGASALTDKNIDVPVDGIEKGVVPITYV
                     PFRNGIFLSIASAIAEKENASAIYIGVVEEDSSGYPDCSDSFIQKMQNAINEGTKEDT
                     KIEIITPLVHLSKAQIVEEAIKLNVPLELTWSCYKEEEKACGVCDSCRLRLNGFKEAN
                     QKDLIPYKES"
     gene            972..1499
                     /locus_tag="PKO10_03115"
     CDS             972..1499
                     /locus_tag="PKO10_03115"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014475238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HNW66172.1"
                     /translation="MWEISKEFDFCYGHRVWSQSLNVEFSLDSCLMCRHLHGHQGKVI
                     VYLKSDTLKSGMVTDFKHLNWFKKFLDDVIDHKFIIDINDPLFKTLLPNIDKKDLISF
                     KEGYKTVDLTNFKNELLELYESYIIVDFVPTSENISAWLLKIVQNKMDKLNIKVSKVE
                     FLETPKSKSTFYARS"
     gene            1486..2250
                     /locus_tag="PKO10_03120"
     CDS             1486..2250
                     /locus_tag="PKO10_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HNW66173.1"
                     /translation="MLEVNEIFGPTIQGEGKLVGNLSIFIRFGKCNFKCVGFNVEYET
                     PSGVKKCACDSYYAVDMEFKNSWKSYENYLDIVNEVDKLLENCNYKNRVDIVITGGEP
                     LLYWNNQEFQNLIKHYINKNHKLTIETNASLNIDFQEEYQKNILFSMSVKLSNSLEPL
                     KKRVNKSTLTKILENTKESYLKFVIGNDFLENAIVEIEDILKNIPQCEVYLMPLGDSS
                     ETIDINSEKVVNLAIKYGFKYSDRLHIRIWNNKRGV"
BASE COUNT          927 a          230 c          372 g          802 t
ORIGIN      
        1 aaattactac agcattgctc aaatgatata ttggtgtcat aaattttaat ctttttgcca
       61 aagatataaa atcttttttt gtcacaacag agtataaatt aaataacatt attcctaaaa
      121 aaatatatat cgcataaata tgagttgtta tcgcactttg cattagttcc atattaagtt
      181 tatctcactt ttaaaaattt ttgctattat atccaactta taataagttt atatttttca
      241 atattttcat acatttttat acaatataag gattttttag tttatgaatc aaaattttaa
      301 taaaaaagca gttgtaatat taagtggtgg aatggattcc actttaagct cttatattgc
      361 aaaaaatagt ggttatgaat taatagcaat tcactttaac tatggtcaaa gaactcaaga
      421 tagagaatta aaggcattta gagatatttg taaagagtta aatgttttaa ataaatatga
      481 gattgatatt ccattttttt ctcaaattgg agctagtgct ttaactgata aaaatataga
      541 tgttccagta gatgggattg aaaaaggtgt tgttccaatt acttatgttc catttagaaa
      601 tggaatattt ttaagtatcg caagtgctat tgcggaaaaa gagaatgcaa gtgcaatcta
      661 tattggtgta gtagaagagg atagttcagg ttatccagat tgtagtgata gctttataca
      721 aaaaatgcaa aatgctataa atgaaggtac aaaagaggat acaaaaatag agataataac
      781 tcctcttgtt catttaagta aagctcaaat tgtagaagaa gctataaaat taaatgttcc
      841 actagaactt acttggtctt gctacaaaga agaggaaaaa gcttgtggag tttgtgactc
      901 ttgcagactt cgattaaatg gatttaaaga ggcaaatcaa aaagatttaa taccttataa
      961 ggagagttaa aatgtgggaa atttcaaaag agtttgactt ttgttatgga catagagttt
     1021 ggtcacaatc attaaatgtt gagttctcac ttgatagctg tcttatgtgt aggcatcttc
     1081 atgggcacca aggtaaggta attgtatatc taaagtctga tactctaaaa agtggaatgg
     1141 ttacagattt taaacattta aattggttta aaaaattttt agatgatgtt attgatcata
     1201 agtttataat agatataaat gatccactat ttaaaactct actaccaaat atagataaaa
     1261 aagatttaat aagttttaaa gaaggctaca aaacagtaga tttaacaaat tttaaaaatg
     1321 agctattaga actttatgaa agttatatta ttgtagattt tgttccaaca agtgagaata
     1381 tttcagcatg gttgttaaaa attgttcaga ataaaatgga taaattaaat ataaaagtat
     1441 caaaagttga atttttagag actccaaaaa gtaaaagtac tttttatgct agaagttaat
     1501 gaaatttttg gaccaacaat tcaaggagaa gggaaattag taggaaatct ctcaattttc
     1561 attcgatttg gtaaatgtaa ttttaaatgt gttggattta atgtagagta tgaaactcca
     1621 agtggagtta aaaagtgtgc ttgtgactca tattacgctg ttgatatgga gtttaaaaat
     1681 agttggaaaa gctatgaaaa ttatcttgat atagtaaatg aagttgataa acttcttgaa
     1741 aattgtaatt ataaaaacag agttgatatt gtaataacag gtggcgaacc acttttatat
     1801 tggaataatc aagagtttca aaatttaata aaacattata ttaataaaaa tcataagcta
     1861 acaatcgaaa caaatgcctc tttaaatata gattttcaag aggagtatca aaaaaatatt
     1921 ctattttcaa tgagtgtaaa attaagcaac tctttagagc ctttaaaaaa aagagttaat
     1981 aaatctacat taacaaaaat tttggaaaat acaaaagagt cttatcttaa atttgtaatt
     2041 ggtaatgatt ttttagaaaa tgcaatcgtt gaaattgaag atatattaaa gaatattcca
     2101 caatgtgagg tttatttaat gccacttggt gatagttctg aaactattga tataaactct
     2161 gaaaaagttg taaatctagc aattaaatat ggatttaaat actctgatag attgcatatt
     2221 agaatttgga ataataaaag aggagtttaa tataatcaaa atattgatat aataaatctt
     2281 tatgcaaaaa aataaaagga taaaaatatg aaatttagtg gaaaaaatgt t
//
LOCUS       DAOQAN010000145         2331 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_66627_length_2331_cov_2.474077, whole genome shotgun sequence.
ACCESSION   DAOQAN010000145 DAOQAN010000000
VERSION     DAOQAN010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66627_length_2331_cov_2.474077"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="PKO10_03125"
     CDS             complement(<1..404)
                     /locus_tag="PKO10_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HNW66174.1"
                     /translation="MKTVELELEELYFQKQKLEEKIKELENFLKNQKSKDKKEFSKDE
                     KIELFRELFISRTDIYAKKWKSKDGTKEGFSPVSKTFMGDDFLPLTNKDLEEHLRGNI
                     FLASYLIDKKQECKYVVLELNSEDVFKLQRALL"
     gene            complement(391..1563)
                     /locus_tag="PKO10_03130"
     CDS             complement(391..1563)
                     /locus_tag="PKO10_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014473249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HNW66175.1"
                     /translation="MSLKTKSLSGFLDHKTMYYDKIDFSFVNRAFSILEKEIKIPFVI
                     HIVGTNGKGSTGRFLSHYLYKTGFKTLHYSSPHIKKFNERIWINGADVSDNLLQNAHE
                     FLTRIYDEELLLKLTYFEYTTLLALYLSKDCEYLVLEAGLGGEFDATNVVKNDLSLIT
                     TIGLDHISFLGNSIEKIARTKMRATNKKMIIGYQEFQEVFEVAQKVKEELKEEFNTDI
                     EILKVENFEIDYDFVFASYLKRNLALSIRCLQELPDFKNKPINLEIFNTTPLFGRCQK
                     IGKNLYIDVGHNSLAAKVIKEEFKDKKITLIYNSYEDKDYFEVLKVLKPIIKEIFILP
                     LDDKRIVKKDILKKCIKELELIEIEKLAMDLENDYLVFGSFLVVENFLGLIGYENS"
     gene            complement(1566..2081)
                     /gene="lptE"
                     /locus_tag="PKO10_03135"
     CDS             complement(1566..2081)
                     /gene="lptE"
                     /locus_tag="PKO10_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HNW66176.1"
                     /translation="MRFLKPISLLLVSILFLASCGYRPSAYYAKQELGKDLFVRLDVT
                     PSDPKNSVLVKDAVTKILIQRVGSNMVNSESQADIIMDLKIASVSFSTLQYDKDGYNK
                     LYKANVVIAVKYFDKSTSKTKSFTVDGEYDFAVDIGSSINDTQRFEAISKASDMAVTD
                     ILSRVAVSSFK"
     gene            complement(2083..>2331)
                     /locus_tag="PKO10_03140"
     CDS             complement(2083..>2331)
                     /locus_tag="PKO10_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013134467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66177.1"
                     /translation="EASPAITDLDDELKNEVFEKDSIVLAVTVNGKKRCEIEVPLNSS
                     NEEVLLIAKESAKKWLENSTIIKEIVVPNKLVNIVIKA"
BASE COUNT          781 a          397 c          229 g          924 t
ORIGIN      
        1 agcaaagccc tttgaagttt aaaaacatct tcactgttta actctaaaac tacatattta
       61 cactcttgtt ttttatctat taaatatgaa gctaaaaaga tatttcctct taaatgttct
      121 tctaaatctt tatttgtaag aggtaaaaaa tcatcaccca taaatgtttt actaacagga
      181 gaaaagccct ctttagttcc atctttactc ttccattttt tagcataaat atcagttcta
      241 gatataaaaa gttctctaaa aagctctatt ttctcatctt ttgaaaactc ttttttatct
      301 tttgattttt gattttttaa aaagttttct aactctttaa ttttttcttc aagcttttgt
      361 ttttgaaagt ataactcttc taactcaagc tcaactgttt tcataaccta ttagtcccaa
      421 aaaattttca acaactaaaa aagagccaaa aaccaaataa tcattttcta aatccattgc
      481 taatttttca atctcaataa gttctaactc ttttatacac tttttcaaaa tatctttttt
      541 aacaattcgt ttatcatcta aaggcaatat aaaaatctct tttatgattg gttttaaaac
      601 ttttaaaact tcaaaataat ctttatcttc ataagagtta taaatcagag ttattttttt
      661 atctttaaac tcctctttta taacttttgc tgccaaagag ttatgtccaa catctatata
      721 taaatttttt cctatttttt gacatcttcc aaatagagga gttgtattaa aaatttcaag
      781 atttataggc ttatttttaa aatctggtaa ctcttgtaaa catctaatac taagagccaa
      841 atttcttttt aagtatgaag caaaaacaaa atcataatct atttcaaagt tttcaacttt
      901 taagatttca atatctgtat taaactcttc ttttagctct tcttttactt tttgtgcaac
      961 ttcaaaaacc tcttgaaact cttggtaacc tattatcatt tttttgtttg ttgctctcat
     1021 ttttgttctt gcaatttttt ctatgctatt tcccaaaaaa gatatgtggt caagccctat
     1081 agttgtgatt aaacttaggt catttttcac cacatttgta gcatcaaact ctccacctaa
     1141 tcctgcttca agaactaaat attcacaatc tttacttaaa taaagagcta aaagagttgt
     1201 atattcaaaa tatgttagtt ttaaaagtaa ctcttcatca taaattctag tcaaaaattc
     1261 atgtgcattt tgtaataaat tatcactaac atcagctcca tttatccaaa ttctctcatt
     1321 aaattttttg atatgagggg agctataatg aagagtttta aagccagttt tatataaata
     1381 gtgagataaa aatcttcccg tgcttccttt tccatttgtt ccaacaatat gaataacaaa
     1441 aggaattttt atctcttttt ctaaaataga aaaagctctg tttacaaagc taaaatctat
     1501 tttgtcataa tacatggttt tatgatctaa aaaaccactt aatgattttg tttttaaact
     1561 catttttatt taaaagaact aacagcaact cttgaaagaa tatcagtaac tgccatatca
     1621 ctagcttttg aaatagcttc aaacctttgt gtatcgttta tactacttcc aatatcaact
     1681 gcaaaatcat actcaccatc aactgtaaaa ctctttgttt ttgatgtaga tttatcaaaa
     1741 tatttaacag ctattactac atttgctttg tacaatttat tgtaaccatc tttatcatat
     1801 tgtaaagttg agaagctaac acttgcgatt tttaaatcca ttataatatc agcttgagac
     1861 tcactattta ccatattaga accaactctt tgaattaata tcttagttac agcatctttt
     1921 actaaaacag agtttttagg atcagatggt gttacatcca atcttacaaa taaatcttta
     1981 cctaactctt gttttgcata gtatgcagat ggtctatatc cacaagatgc caaaaataat
     2041 atagatacta aaagtaaaga aattggtttt agaaatctca tattatgcct taattacaat
     2101 atttacaagc ttatttggca caacaatctc ttttataatt gtagagtttt ctaaccattt
     2161 tttagcactc tcttttgcta ttaataaaac ctcttcattt gagctattta gaggtacttc
     2221 tatttcacat ctttttttac catttactgt aacagctaaa actatactat ctttttcaaa
     2281 aacctcattt ttaagctcgt catcaagatc tgtaatcgca ggtgatgcct c
//
LOCUS       DAOQAN010000146         2328 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_66751_length_2328_cov_2.725473, whole genome shotgun sequence.
ACCESSION   DAOQAN010000146 DAOQAN010000000
VERSION     DAOQAN010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66751_length_2328_cov_2.725473"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="PKO10_03145"
     CDS             <1..377
                     /locus_tag="PKO10_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HNW66178.1"
                     /translation="DIQKNVNFYLKSKNEYIKEEYNILRKELAEMLIDINTLSMLEND
                     SDQLTQFELIKTELAHNDLASSEELDTLIREDKIKATMATSYMNDSATGYSIQKKLVK
                     VANILFLNDGIVKQIGDENETK"
     gene            364..597
                     /locus_tag="PKO10_03150"
     CDS             364..597
                     /locus_tag="PKO10_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66179.1"
                     /translation="MKLSKIVDKVKKYLEKDNLKVSQEEKLLNIIEELENKKSKIKDE
                     LKNIDKDNIKKRVELEKKYNAVSKVLKKSRSIL"
     gene            complement(587..1882)
                     /gene="brnQ"
                     /locus_tag="PKO10_03155"
     CDS             complement(587..1882)
                     /gene="brnQ"
                     /locus_tag="PKO10_03155"
                     /inference="COORDINATES: protein motif:HMM:NF017347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HNW66180.1"
                     /translation="MKTSNITRDTLILGFAIFSMYFGAGNIIFPPYLGLISSSDWKIS
                     FFAYFLADIGFATFAMFALLKVGGNVDNLTSKLGTINGKILMAIIILCIGPLIALPRT
                     GAVTYEMLVVPYFGASTFNSLITSIIYFGLILFFTLRPTSMIEILGKVLSPLLFLSLI
                     ILIIKGIFTPIGEISQNTTNDKLFFDGLLLGYQTLDILAALAFGIIIIKILKTKGYTN
                     KKTNFKIVGYASLIAAFGIMLVYFGLTYLGATSSLVYETNIEKVTLLNNIIYQLFGSN
                     GAIILAFVVFLACFTTGSALVSVTAEYFSKVSQGRFSYKKLVILTSLFAVVVTNFGLE
                     TIINFAAPILFIVYPAAIILVILAFFDNYIDNLNIYKFSSFGAIIYSIFELISNSYLK
                     LSFMENIPLAKEGLGWILPAIIFGIIGFLIKSKRVRSKV"
     gene            complement(1882..>2328)
                     /locus_tag="PKO10_03160"
     CDS             complement(1882..>2328)
                     /locus_tag="PKO10_03160"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012083463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HNW66181.1"
                     /translation="PRMPGNVGTIGRTAVALKCKLHLIKPYGFGDITEKEVRRAGLDY
                     WKDLELFEYENIEDFWAKNPFSSRHFFATTKTTQPYFEAKFEAGDYIYFGREDAGLPS
                     YILDKNKESCITIPMAKIARSLNLSNSVSIICYEALRQNYINFETI"
BASE COUNT          945 a          321 c          294 g          768 t
ORIGIN      
        1 gagatattca aaaaaatgta aatttttact taaaaagtaa aaatgagtat atcaaagaag
       61 agtataatat tctaagaaaa gagttagcag agatgctaat agatataaat actctttcta
      121 tgttagaaaa tgattcagac caattaactc aatttgaact tataaaaact gagttagcac
      181 ataatgattt agcaagtagt gaagagctag acacacttat aagagaagat aaaataaaag
      241 caacaatggc aacttcatat atgaatgata gtgctacggg ttactctatt cagaaaaaac
      301 ttgtaaaagt tgctaatatt ttattcttaa atgatggtat agttaaacaa ataggagatg
      361 aaaatgaaac taagtaaaat tgttgataaa gtaaaaaaat atttagaaaa agataatcta
      421 aaagtttctc aagaggaaaa acttctaaat ataatagaag agcttgaaaa taagaaaagt
      481 aaaataaaag atgaattaaa aaatatagat aaagataata ttaaaaaaag agttgagcta
      541 gaaaaaaagt ataatgctgt ttctaaagtt cttaaaaaga gccgttctat actttagaac
      601 gaactctttt acttttaatt aaaaacccaa taattccaaa aatgattgct ggtaaaatcc
      661 agcctaatcc ctcttttgcc aaaggaatat tttccataaa tgataacttt aaataactat
      721 ttgatataag ttcaaaaatt gagtatataa ttgctccgaa acttgaaaat ttgtagatat
      781 ttaaattgtc tatatagtta tcaaaaaatg ccaaaataac taaaataata gcagctggat
      841 atactataaa taaaattggt gctgcaaaat taattatagt ttctaagcca aaatttgtaa
      901 caactactgc aaaaagtgaa gttaaaataa ccaatttttt ataactaaac cttccttggc
      961 ttacttttga gaaatactca gcagtaacac ttacaagtgc agatcctgtt gtaaaacaag
     1021 ctaaaaatac tacaaatgct aaaataatag ctccattgct tccaaaaagt tggtatataa
     1081 tattatttaa aagtgttact ttttcaatat tagtttcata aactaaacta gaagttgctc
     1141 ctaaatatgt caaaccaaaa tatacaagca ttattccaaa agcagcaatt aaagaagcat
     1201 atccaactat tttaaagttt gtttttttgt ttgtataacc ttttgttttt aatattttta
     1261 taataataat tccaaatgct aaagctgcta aaatatctaa agtttgataa cctaaaagaa
     1321 gaccatcaaa aaatagttta tcatttgtag tgttttgtga tatctctcca attggagtga
     1381 aaattccttt tattattaaa ataattaaag ataaaaatag caaaggagat aatacctttc
     1441 ccaatatttc aatcatagaa gttggtctta atgtaaaaaa tagaattaaa ccaaaataaa
     1501 ttatagatgt gattaaagag ttaaaagtgc tagctccaaa atatggaact accaacatct
     1561 cataagttac agctcctgtt cttggaagtg caattaatgg accaatacat aaaataatta
     1621 ttgccataag tatttttccg ttaattgttc ctaatttaga agttaaatta tctacatttc
     1681 ctccaacttt aagcaaagca aacatagcaa atgtagcaaa tccaatatcg gctaaaaaat
     1741 atgcaaaaaa agatattttc caatcacttg aactaattaa tcccaaatat ggtggaaaaa
     1801 taatatttcc agccccaaaa tacatagaga aaattgcaaa tcctaagatt aatgtatctc
     1861 ttgttatgtt agatgttttc attaaatagt ctcaaaattt atatagtttt gtcttaaagc
     1921 ttcataacaa attatagata cagagtttga gagatttaaa cttcttgcaa tttttgccat
     1981 tggaattgtt atacaactct ctttattttt atctaaaata tatgaaggta atcctgcatc
     2041 ttctcttcca aaatatatat aatcccctgc ttcaaatttt gcctcaaaat aaggttgagt
     2101 tgtttttgtt gtagcaaaaa aatgtctaga agaaaatgga ttttttgccc aaaaatcttc
     2161 aatattttca tactcaaaaa gttctaaatc tttccaataa tcaagtcctg ctcttcgcac
     2221 ctctttttca gttatatctc caaaaccata aggttttatt aaatgaagtt tacattttaa
     2281 agcaacagca gttcttccta tcgttcctac gtttcctggc attcttgg
//
LOCUS       DAOQAN010000147         2320 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_67077_length_2320_cov_3.749669, whole genome shotgun sequence.
ACCESSION   DAOQAN010000147 DAOQAN010000000
VERSION     DAOQAN010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67077_length_2320_cov_3.749669"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1446)
                     /locus_tag="PKO10_03165"
     CDS             complement(<1..1446)
                     /locus_tag="PKO10_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNW66182.1"
                     /translation="MRDIRNIAVIAHVDHGKTTLVDQLLKQSGTFSAHQNVDERVMDS
                     NAIEKERGITILSKNTAIDYEGVRINIIDTPGHADFGGEVERVLKMVDSVLLLVDAQE
                     GVMPQTKFVVKKALQLGHRPIVVVNKIDKPAAEPDRVVDEVFDLFAQMDATEEQLEFP
                     VIYAAARDGVARYDANDGNMDFKPLYDTILKEVPKPQGADENGLQLQVFTLDYDNFIG
                     KIGIARIFNGTISHGETVMLCKADGEKIKGRVSKLIGFKGMDRFDIKTAGSGDIVAVA
                     GFETIDVGDSLCDPNNPMPLDPMHIEEPTLSVTFSVNDSPLAGTEGKFVTSNKIDERL
                     KAEMNTNIAMSYEQIGEGKFKVNGRGELQITILAENMRREGFEFCIGRPEVIIREENG
                     IKMEPFEHLVIDTPDEFSGAIIEKLGKRKAVMTNMVPMGEGSTRLEFEIPARGLIGIR
                     TEFLTETRGEGVMNHSFLEFRPHSGTVESRKY"
     gene            1699..2223
                     /locus_tag="PKO10_03170"
     CDS             1699..2223
                     /locus_tag="PKO10_03170"
                     /inference="COORDINATES: protein motif:HMM:NF016064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HNW66183.1"
                     /translation="MEFLLNFRKTLSRFWSPIPAVIALGVLSAYYFGITGTYWAVTGE
                     FTRWGGHFLQLFGVDVSTWGYYKLMSIQGNIFTRVDGVMIIGMFAGCIAAAFWGNNVK
                     FRLPLNNIRIYQALIGGIIAGFGARLGMGCNLASFFTGIPQFSFHAWVFTAFMMVGVY
                     FGVKVALSPFFNQK"
BASE COUNT          703 a          425 c          334 g          858 t
ORIGIN      
        1 atattttcta ctttcaactg ttccactatg tggtctaaac tctaagaaag agtgattcat
       61 aacaccctct cctctagttt cagttaaaaa ttctgttcta attccaatta atccacgtgc
      121 aggaatctca aactccaatc tagtagaacc ctctcccatt ggaaccatgt ttgtcataac
      181 agcttttctt tttccaagtt tttcaataat agcaccacta aactcatctg gagtatcaat
      241 tactaaatgc tcaaatggct ccattttaat cccattttct tctctaataa taacttcagg
      301 tcttccaata cagaactcaa aaccttctct tctcatattc tcagctaaaa tagtaatttg
      361 aagttcacct cttccgttta ctttaaattt accttcacca atttgctcat aactcatagc
      421 aatatttgtg ttcatttcag cttttaatct ctcatcaatt ttatttgaag ttacaaattt
      481 accttcagtt ccagcaagtg gagagtcatt tacagaaaaa gttacactta aagttggttc
      541 ttcaatatgc atagggtcaa gtggcattgg attatttgga tcacataaag aatccccaac
      601 atcaatagtt tcaaacccag caactgcaac aatatctcca gaaccagcag ttttgatatc
      661 aaatctatcc attcctttaa acccgataag ttttgatact cttcctttta ttttttctcc
      721 atcagcctta cacaacatta ctgtttcacc gtggcttatt gttccattaa atattctagc
      781 tattcctatt tttccaataa agttatcata atctagtgta aatacttgaa gttgaagacc
      841 attttcatca gccccttgag gttttggaac ttcttttaaa attgtgtcat atagtggttt
      901 aaaatccata ttcccatcat ttgcatcata tcttgcaaca ccatctcttg cagctgcata
      961 aataactggg aactctaact gctcttctgt tgcatccatt tgcgcaaata aatcaaaaac
     1021 ttcatcaaca actctatctg gttcagctgc tggtttatca attttgttta caacaacgat
     1081 tggtctatga cctagttgaa gtgctttttt aacaacaaat tttgtttgtg gcataacacc
     1141 ctcttgtgca tctacaagaa gtaaaacaga atcaaccatt tttaaaaccc tctcaacttc
     1201 tccaccaaaa tcggcgtgtc ctggagtatc aataatgtta attcttacac cttcataatc
     1261 aatagctgta ttttttgaaa gaattgtaat tcctctctct ttctcgatag cattactatc
     1321 cataactctt tcatctacat tttggtgagc tgaaaatgta ccactttgtt ttaatagttg
     1381 atctactagc gtagttttac cgtggtcaac gtgtgcaata actgcaatat ttctaatgtc
     1441 tctcatactt cttctttttt tgtaaaattt tcgcggattg tagctaaaaa aaacttaaaa
     1501 gcttataata gcttaaaata tggttaagca aagaataaat attgagtaaa agttttttta
     1561 cctttttata ttaaattaat attattttgt taacattcgc aaattttata ggtttgcggt
     1621 gcaaacagac aacttgaaac tcttttttta cttactcaag tgtcaatcaa aaatattttt
     1681 tattgtcaaa ggacttttat ggagttttta ctaaatttta gaaaaaccct ttcaagattt
     1741 tggtcaccaa ttccagcagt tattgcactt ggagttttga gtgcttacta ttttggaata
     1801 acaggaactt actgggctgt tacaggtgaa tttactagat ggggtggtca ctttttacaa
     1861 ctatttggag ttgatgtttc aacttgggga tattataaac ttatgagtat tcagggtaat
     1921 atctttacaa gagttgatgg tgttatgatt attggtatgt ttgctggatg tatcgcagct
     1981 gctttttggg gaaataatgt aaagtttaga ttacctctaa acaatattag aatttatcaa
     2041 gccttaattg gtggaattat tgcgggattt ggagcaagac ttggaatggg ttgtaattta
     2101 gcaagttttt ttacaggaat tcctcaattt agttttcacg cttgggtatt tacagcattt
     2161 atgatggttg gagtttactt tggagtaaaa gttgctctta gccctttttt caatcaaaaa
     2221 taaagatgca aaaagtatct tgtgcaaaac cacttgaaca caatgaagag aaagttaaaa
     2281 aattctttac ttttggaaca tttgcattta ttgcaattat
//
LOCUS       DAOQAN010000148         2304 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_67830_length_2304_cov_2.178301, whole genome shotgun sequence.
ACCESSION   DAOQAN010000148 DAOQAN010000000
VERSION     DAOQAN010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67830_length_2304_cov_2.178301"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1356)
                     /gene="cysS"
                     /locus_tag="PKO10_03175"
     CDS             complement(<1..1356)
                     /gene="cysS"
                     /locus_tag="PKO10_03175"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HNW66184.1"
                     /translation="MKKIYFYDTSKKTKVEFVSLKPNIAKIYVCGPTVYDNSHLGHAR
                     SAIAFDLLHRVLKANGYEVVFAKNFTDIDDKIIKKMKEEGKSLEEITTFYIKNYKNDM
                     EILNILPNSFEPKATENLDAMKEMIENLLSKDIAYKTDDSVYFDVSKDKNYGSLSNQA
                     SDENSQARVEINNQKRNSSDFALWKFEKASDVSFASNFGAGRPGWHIECSAMIEKHLA
                     YKDEPFQIDIHCGGADLLFPHHENEASQTRCASGQNLAKYWMHNGFVNINGEKMSKSL
                     GNSFFLKDVLKSYSGEVVRFYLLSSSYRANINFNEEDLLASKKRLDKLYRVKKRVYEV
                     TASKVNEKFENTLLEALNDDLNTSLALSFIDEFINSSNDSLDKNPKDKSLKQEIVANI
                     EFIEKVLGFGGSDAFSYFQFGVSLELKKEIEDLISKRAEAKKNKDFAKADSIRDELSA
                     MN"
     gene            complement(1358..>2304)
                     /gene="nusA"
                     /locus_tag="PKO10_03180"
     CDS             complement(1358..>2304)
                     /gene="nusA"
                     /locus_tag="PKO10_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014473096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HNW66185.1"
                     /translation="VNEDGTPINKENFIAIDEAKAINSDLELGDHLSYELEFENMGRN
                     AATILSSNFEYKLQRFVEENIMAKYKEKVGKTVSAVVTRVDKNDSTFIEIGEIKGILQ
                     RKNRIKGEYFKVGDTLKAVVKSVNIDKNLGLMVELSRTSPKFLENLLTLEVPELKDEK
                     IIIESSARIPGSRSKIALISTSPQIDAIGAIVGVKGVRINAVSKELNGENIDCIEYSS
                     VPEMFIARALSPALVNSVKIEEHPKNGEKGKAVVTINSEQKSKAIGKAGLNIRLASML
                     TKYDIEIVEIETGVTTSNLETKKDEKISDASSLEALFK"
BASE COUNT          712 a          423 c          225 g          944 t
ORIGIN      
        1 attcatagca gataactcat ctcttatact atctgctttt gcaaaatctt tatttttttt
       61 cgcctctgct ctttttgaaa tcaaatcttc tatctctttt tttagctcta aactaactcc
      121 aaattggaaa taagagaaag catcacttcc accaaaacct agaacttttt caataaactc
      181 aatatttgca actatctctt gttttagact cttatctttt ggatttttat ccaaactatc
      241 atttgaacta tttataaact catcaataaa gctaagtgcc aatgatgtat ttaaatcatc
      301 atttagtgct tctaaaagag tgttttcaaa cttttcattc acttttgaag cagttacttc
      361 ataaactctt tttttaactc tataaagttt atcaagtctt tttttacttg caagtaaatc
      421 ctcttcatta aagtttatat ttgctctata tgaagagctt agaagataaa atcttacaac
      481 ttctccacta taagatttca aaacatcttt taagaaaaat gagttcccta aagatttact
      541 catcttttct ccatttatat ttacaaaacc gttgtgcatc caatattttg caagattttg
      601 accgcttgca catcttgttt gactagcttc attttcgtga tgaggaaaaa gtaaatcagc
      661 tccaccacaa tgaatatcaa tttggaaagg ttcatcttta taagctaaat gtttttcaat
      721 catagcacta cactcaatat gccatccagg acgacctgct ccaaaatttg aagcaaaaga
      781 tacatcacta gctttttcaa atttccaaag agcaaaatct gaactatttc ttttttgatt
      841 gtttatttca actcttgctt gtgaattttc atcacttgct tgatttgata atgaaccata
      901 atttttatct tttgaaacat caaaatatac actatcatct gttttatatg caatatcttt
      961 tgaaagtaaa ttttctatca tctctttcat agcatcaaga ttttcagtag cttttggctc
     1021 aaaactattt ggtaaaatat ttaaaatttc catatcattt ttataatttt ttatataaaa
     1081 agttgtaatc tcttctaaag atttaccctc ttctttcatc tttttgataa tcttatcatc
     1141 aatatcagta aaattttttg caaaaactac ttcataacca tttgctttta aaactctatg
     1201 aagtaaatca aaagcaatag cacttcttgc atgtcctaag tgagaattat cataaacagt
     1261 tggtccacat acatatattt ttgcaatatt tggttttaaa gatacaaatt ctacttttgt
     1321 tttttttgat gtatcataaa aatatatctt tttcatacta tttaaatagt gcctctaggc
     1381 ttgatgcatc acttatcttt tcatcttttt tagtttcaag atttgaagtt gttacacctg
     1441 tttcaatctc aacaatctct atatcatatt ttgtaagcat agaagctaat cttatattta
     1501 atccagcttt tcctattgct ttactttttt gctcactatt aattgtaaca actgctttac
     1561 ctttttcacc attttttgga tgttcttcta tttttacact atttactaaa gcaggactta
     1621 aagcacgagc aataaacatc tctggaacac ttgaatactc tatacaatct atattttctc
     1681 catttagctc ttttgaaact gcatttattc taacaccttt tacaccaaca attgctccaa
     1741 ttgcatctat ttgtgggctt gtagatataa gtgctatttt tgatctgctt ccaggaattc
     1801 tagcacttga ttctataata attttttcat ctttaagctc tggaacttct aaagtaagta
     1861 gattttccaa aaacttagga cttgttcttg ataactctac cattaatccc aaatttttat
     1921 caatatttac agatttaaca acagctttaa gtgtatctcc tactttaaaa tactcacctt
     1981 ttattctatt ttttctttga agaatacctt ttatttctcc tatttcaata aaagtactat
     2041 catttttatc aactcttgta acaacagcag atacagtttt tccaactttt tctttatatt
     2101 ttgccattat attttcttct acaaatcttt gaagtttata ttcaaaatta cttgaaagaa
     2161 ttgttgctgc atttcttccc atattttcaa attctaactc ataactcaaa tgatccccaa
     2221 gttctaaatc actattgata gcttttgctt catctattgc tataaaattc tctttattta
     2281 taggagttcc atcttcattt acta
//
LOCUS       DAOQAN010000149         2303 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_67860_length_2303_cov_3.319840, whole genome shotgun sequence.
ACCESSION   DAOQAN010000149 DAOQAN010000000
VERSION     DAOQAN010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67860_length_2303_cov_3.319840"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2102)
                     /gene="metE"
                     /locus_tag="PKO10_03185"
     CDS             complement(<1..2102)
                     /gene="metE"
                     /locus_tag="PKO10_03185"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014469331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HNW66186.1"
                     /translation="MSKNYVIGFPRIGEKRELKKVLEDYWAKKVDFSEVKYVAENLRK
                     RHWTYQKEAKIDFIASNDFSLYDNMLDSSILLGAIPKRFQHLKDEELYFAMARGNQDC
                     VAMEMTKWFNTNYHYIVPEISKDTTFKLNSKKVIEEYKEALELGINTKINLIGAITYL
                     GLSKSVDNSDLFAHISSVVKVYKELLEEISKLNSEIVVQFDEPLFVKDLEPKVLSLLK
                     PVYDELSNVSKNIKIVVTTYFEHSNEATKILVNTPIWALGLDFIHGVKNFETLEFIKN
                     SNKVLIAGVIDGRNIWKSNFEDKLNLLNKISNVVSKDNIIVGTSCSLLHVPFTLSYED
                     NLDKEIKSWLAFANEKLKELNLVSKQFFGSKLSLEDIANIEKNRQDNIQRKVSTKIHN
                     QKIQEEIKNLKKFEREDKFEDRIKAQREFFKYDFLTTTTIGSFPQTPEIRENRKNYKA
                     NLISKEEYETQIKKYIDDCVAFQDEIGLDILVHGEPERNDMVEYFGQLLEGFAFTQNA
                     WVQSYGSRCVKPPVIFGDVSRPKAMTVEWIKYAQSKTSKVMKGMLTGPVTILNWSFVR
                     DDIPRNEVTKQIALCINKEVDDLQNAGIKMIQVDEAAFKEGYPLRAENIKAYENWAVD
                     NFRLSVSSAKADTQIHTHMCYSEFNDIIKTIEAMDADVISIETARSGNRLLKIFKEVN
                     YKQEIGPGIYDIHSPRVP"
BASE COUNT          756 a          383 c          241 g          923 t
ORIGIN      
        1 cttggaactc taggactatg aatatcatat attccaggtc ctatttcttg tttatagttt
       61 acctctttaa agatttttaa aagtctattt ccactacgtg cagtttcaat agagataaca
      121 tcagcatcca tagcttcaat agtttttata atatcattaa actcactata acacatatgt
      181 gtgtgaattt gagtatcagc ttttgcgcta cttacactta atctaaaatt atcaactgcc
      241 caattttcat aagcttttat attttcagct cttaaaggat aaccctcttt aaatgcagct
      301 tcatcaactt gaatcatctt aattccagca ttttgtaaat catctacttc tttattaata
      361 caaagtgcaa tttgttttgt tacttcattt cttggaatat catctcttac aaatgaccaa
      421 tttaaaattg taacaggtcc tgttaacata cctttcataa ctttagaagt tttactttga
      481 gcatatttaa tccactcaac agtcatagcc tttggtcttg aaacatcacc aaaaataaca
      541 ggtggtttta cgcatctact tccatatgat tgaacccaag cattttgtgt aaatgcaaaa
      601 ccttccaata attgcccaaa atattcaacc atgtcatttc tttcaggctc tccgtgaact
      661 aaaatatcaa gtccgatttc atcttgaaat gctacacaat catcaatata tttttttatt
      721 tgagtttcat actcttcttt tgaaattaaa tttgctttat aattctttct attctctcta
      781 atttctggag tttgaggaaa tgatccaata gttgtagttg ttaaaaaatc atatttaaaa
      841 aactctcttt gagcttttat tctatcttca aatttatctt ctctttcaaa tttttttaga
      901 tttttaatct cttcttgaat cttttgatta tgaattttag tagatacttt tctttgaata
      961 ttatcttgtc gatttttctc aatatttgca atatcttcaa gtgatagttt tgagccaaaa
     1021 aactgttttg aaacaaggtt taactctttt aatttttcat ttgcaaatgc taaccacgat
     1081 ttaatctctt tatctaaatt atcttcataa cttagagtaa aaggtacatg taaaagtgaa
     1141 caagaagtcc ctacaataat attatctttt gaaactacat ttgagatttt gtttaaaaga
     1201 tttagtttat cttcaaaatt actcttccaa atgtttctac catcaataac tccagcaatt
     1261 aaaactttat ttgaattttt aataaattct agagtttcaa aatttttaac tccatgaata
     1321 aaatcaagtc ctaaagccca aataggagtg tttactaaaa tttttgtagc ttcatttgag
     1381 tgttcaaagt aagtagttac aacgattttt atattttttg aaacatttga gagctcatca
     1441 taaactggtt ttaaaagtga taaaactttt ggttctaaat cttttacaaa caaaggctca
     1501 tcaaattgaa caacaatttc actgtttaat tttgaaatct cttctaaaag ctctttataa
     1561 acttttacaa cactagaaat atgtgcaaat agatcactat tatcgacact ttttgataat
     1621 cctaaataag taattgcacc aattaaattt atttttgtat taattccaag ctcaagtgct
     1681 tctttatact cctctataac ttttttagag tttagtttaa atgttgtatc tttagaaatt
     1741 tctggaacaa tgtagtgata gtttgtatta aaccactttg tcatctccat tgctacacaa
     1801 tcttgatttc ctcttgccat tgcaaaatat aactcttcat cttttaggtg ttgaaatctt
     1861 tttggaattg ctcctaataa aatagaacta tctaacatat tatcatatag tgaaaaatca
     1921 tttgaagcta taaaatctat ttttgcctct ttttgataag tccagtgtct ttttctcaaa
     1981 ttttctgcta catatttaac ttcactaaaa tcaacttttt ttgcccaata atcttctaaa
     2041 acctttttta gttctctttt ttctccaatt cttggaaatc ctattacata attttttgac
     2101 attattaatc cttttaataa aaatatttaa ttctctacaa aaaatgtaga taacaaaact
     2161 tgataaatat gaaaaaataa aaatcttatt ttaaatcctc tttcgacaag agtttacaca
     2221 cgattggtac caaaaatcac agatttttac caatcgggta aaattgtctt tttcggactt
     2281 aaaaacgatt taatattaat ttt
//
LOCUS       DAOQAN010000150         2294 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_68304_length_2294_cov_1.902635, whole genome shotgun sequence.
ACCESSION   DAOQAN010000150 DAOQAN010000000
VERSION     DAOQAN010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68304_length_2294_cov_1.902635"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1755)
                     /gene="secA"
                     /locus_tag="PKO10_03190"
     CDS             complement(<1..1755)
                     /gene="secA"
                     /locus_tag="PKO10_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009496036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HNW66187.1"
                     /translation="MLNVFSKVFGTRNDRVVKKYKNIANEITNLEAKYSKLSDDELKA
                     KFNEFKKEVQNDEKSLDSVLKDVFAITREASVRSLGLRPYDVQLIGAMVLNDGNIAEM
                     KTGEGKTLVGAIAVCLNALSGKGVHVVTVNDYLATRDANELKPLYEFLGYSVGALSDA
                     VRDDDTRREQYACDITYGTNSSYGFDFLRDNMVYDLKDKVQRGHHFVIVDEVDSILID
                     EARTPLIISGPTNHKNSNYVKANEIALKLVRGELIEPKNASEKPTTTGDFIVDEKNRA
                     VSLTEEGHIKAEELFGVENLYSIENAMLSHSLDQALKANYIFQKDVDYVVKDDQIIIV
                     DEFTGRLSEGRRFSEGLHQALEAKEKVAIQEESQTLADTTYQNYFRMYKKLSGMTGTA
                     QTEATEFAQIYNLDVVSIPTNVPVKRIDKSDLIYKSEREKFEAVCEKIKYYHEKGQPV
                     LVGTASIEKSEKLHKILSDKKIPHTVLNAKQHEKEGKIIADAGQKGAVTIATNMAGRG
                     VDIKLGGSEDDIDSTNKIKELGGLCVIGTERHESRRIDNQLRGRSGRQGDPGSTQFFI
                     SLEDDLMRLFGSDRITSML"
     gene            1815..>2294
                     /gene="lolA"
                     /locus_tag="PKO10_03195"
     CDS             1815..>2294
                     /gene="lolA"
                     /locus_tag="PKO10_03195"
                     /inference="COORDINATES: protein motif:HMM:NF000663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LolA-like outer membrane lipoprotein chaperone"
                     /protein_id="HNW66188.1"
                     /translation="MFYKLIFTLFIFSSFCFSSNDIQNLKSSQSKFTQTITSSSSDII
                     TYQGEVFIKSSGQILWKYKTPVVKNVYIENNMAIVDEPELEQAIFTTLENEINILKLL
                     KEAKKIDNNNYVSTIDGTKYQISMSSNKIKKITYKDTLDNSVEIIFSNSVQDEPIDDN
                     "
BASE COUNT          720 a          411 c          282 g          881 t
ORIGIN      
        1 aagcattgac gtgattctgt cgcttccgaa caatctcatt aaatcatctt caagagaaat
       61 gaaaaattgt gtagatccag gatctccttg acgaccagaa cgtcctctaa gctgattatc
      121 tattcttcta gattcatgtc tttctgtacc aataacacaa agtccaccta attcttttat
      181 tttgtttgta ctatcaatat catcttcact acctccaagt ttaatatcaa ctccacgtcc
      241 tgccatattt gtagcaattg taacagcacc tttttgacca gcatcagcga ttatttttcc
      301 ctctttttca tgctgttttg catttaaaac agtatgagga atttttttat ctgatagaat
      361 tttatgaagt ttctcactct tttcaatact tgcagttcct acaagtacag gttgtccttt
      421 ttcatggtag tactttatct tttcacaaac ggcttcaaac ttctctcttt cacttttata
      481 aattaaatca cttttatcaa ttcttttaac aggtacattt gtaggaatag atacaacatc
      541 taaattataa atttgtgcga attctgttgc ttctgtttga gctgttccag tcattcctga
      601 aagttttttg tacattctaa agtagttttg atatgttgta tctgctaaag tttgactctc
      661 ttcttgaata gcaactttct cttttgcttc aagtgcttgg tgaagtcctt cgctaaatct
      721 tcttccttcg cttaatcttc ctgtaaactc atctacaata atgatttgat catcttttac
      781 aacatagtca acatcttttt ggaaaatata gtttgcttta agtgcttgat caagagagtg
      841 cgataacata gcattttcaa ttgagtaaag attttcaact ccaaaaagct cctcggcttt
      901 tatgtgacct tcttctgtta aacttacagc tctatttttt tcatctacaa taaaatctcc
      961 agttgtagtt ggtttttcgc tagcattttt tggttctatt agctccccac gaactaattt
     1021 tagtgctatc tcatttgctt ttacatagtt tgaatttttg tgatttgttg gaccactaat
     1081 aattaatgga gttctagctt catctattaa aatagagtca acttcatcta caataacaaa
     1141 atgatgacct ctttgaactt tatcttttaa atcataaacc atgttatctc ttaagaagtc
     1201 aaatccatat gaactatttg ttccataagt aatatcacaa gcatattgtt ctcttcttgt
     1261 atcatcatct cttacagcat cacttaaagc tccaacactg tatcctaaaa attcatatag
     1321 tggttttagc tcatttgcat ctctagttgc taaataatca tttactgtta caacatgtac
     1381 accttttccg cttaatgcat ttaaacaaac tgcaatagca ccaacaagag tttttccctc
     1441 tcctgttttc atctctgcaa tatttccatc atttaaaacc atagcaccaa ttagttgcac
     1501 atcataaggt ctaagaccta aacttctaac actagcttct cttgttattg caaaaacatc
     1561 ttttaaaaca ctatccaatg atttttcatc gttttgtact tcttttttaa actcattaaa
     1621 tttggcttta agctcatcat cgcttagctt tgagtatttt gcttccaaat ttgttatttc
     1681 gtttgctata tttttatatt tttttacaac tctatcattt cttgtaccaa aaacttttga
     1741 aaaaacattt aacataaaat tagttccttt tcgttataat gaagcaatga ttatatataa
     1801 aaaaaggtta aaaaatgttt tataagctta tctttactct gtttattttt tcaagcttct
     1861 gtttttcttc aaatgatatt caaaatctta aaagttctca gtcaaagttt actcaaacta
     1921 ttacatcgag ttcaagtgat ataataactt atcaaggtga agtttttata aaaagtagcg
     1981 gacaaatttt gtggaaatac aaaactccag tggttaaaaa tgtttatatc gagaataata
     2041 tggcaatagt tgatgagcca gaattagagc aagctatttt tacaactctt gaaaatgaga
     2101 taaatatatt aaagctttta aaagaggcta aaaaaattga taacaataac tatgtttcaa
     2161 caatagatgg tacaaaatat caaatttcta tgagtagtaa caaaataaaa aaaataactt
     2221 ataaagatac tcttgataat agtgttgaga taattttctc aaattcagtt caagatgaac
     2281 caattgatga taat
//
LOCUS       DAOQAN010000151         2288 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_68572_length_2288_cov_2.588446, whole genome shotgun sequence.
ACCESSION   DAOQAN010000151 DAOQAN010000000
VERSION     DAOQAN010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68572_length_2288_cov_2.588446"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1901)
                     /gene="aceE"
                     /locus_tag="PKO10_03200"
     CDS             complement(<1..1901)
                     /gene="aceE"
                     /locus_tag="PKO10_03200"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HNW66189.1"
                     /translation="MPKVQLEDVNPLETKEWMEALEAVIDEEGVERAHFLLEKLIDKS
                     RRSGAHLPFKATTAYINSIPKEEEPKIPANMDLERKIRSIIRWNAQIMVQRASNKQLE
                     LGGHIASFQSSATLYDVAFNHFFKAPNEKDGGDLIFFQGHISPGIYARSFLEGRFTQE
                     QMDNFRQEAFADGLSSYPHPKLMPTYWQFPTVSMGLGPLQAIYQARFLKYLTNRGIKD
                     CSGQKVYCFMGDGECDEPEALGAIGMAAREELDNLIFVINCNLQRLDGPVRGNGKIIQ
                     ELEGEFRGAGWEVIKVIWGGLWDNLLEKDTSGKLLELMEQTVDGEYQNFKQKGGAYTR
                     ENFFNKFPETAKLVENLSDNDIWKLNRGGHDPVKVYAAYKRAVETKGRPTVILAKTVK
                     GYGMGSAAEGMNIAHGVKKVDVNQLKAFRDRFDLPISDEEVESYSYYKPDENSPEVQY
                     LKEKRAALGGFVPQRREKFSNKLEIPALSEFESIIAGSGDREISTIMAFVRVLNALLK
                     DKQIGKNIVPIVPDEARTFGMEGMFRQFGIYSSAGQKYIPQDKDQVAFYKEDIKGQVL
                     QEGINELGAMSSWIAAATSYSVNNYPMIPFYIFYSMFGFQRTGDLCWAAGDQKARGFL
                     VGGTSGRTTL"
     gene            2007..>2288
                     /locus_tag="PKO10_03205"
     CDS             2007..>2288
                     /locus_tag="PKO10_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HNW66190.1"
                     /translation="MKINNKFRLIITQNLSSKINSNIDKALFKAFKNDSFPILRLYSW
                     EDCITFGAGQNIDDYKQLQSDYKNNVSKRLTGGGVLFHGHDISYTILLNP"
BASE COUNT          706 a          434 c          293 g          855 t
ORIGIN      
        1 agtgttgttc ttcctgaagt tccacctact aagaaacctc ttgctttttg gtcacctgct
       61 gcccaacata aatctccagt tctttggaac ccaaacattg agtagaatat ataaaatgga
      121 atcattggat aattatttac agaataagat gttgcagctg caatccatga actcatagct
      181 ccaagctcat ttattccctc ttgtaaaact tgacctttta tatcttcttt ataaaatgca
      241 acttggtctt tatcttgtgg aatgtatttt tgtccagcac ttgaataaat tccaaactgt
      301 ctaaacatac cttccatacc aaatgttcta gcttcatcag gaactattgg aacgatattt
      361 tttcctattt gtttatcttt taataaagca tttaaaactc ttacaaatgc cattattgta
      421 gatatttctc tatctccact acctgcaatt atactttcaa attcacttaa ggctggaatt
      481 tcaagtttat ttgagaattt ctctcttctt tgtggtacaa acccaccaag agctgctctt
      541 ttctctttta agtattgaac ttcaggagag ttttcatctg gtttatagta tgaataagat
      601 tcaacctctt catcactaat tggtaaatca aatctatctc taaatgcttt tagttggttt
      661 acatctactt tttttacacc gtgagcaata ttcataccct cagctgcgct tcccattcca
      721 taacctttta cagtttttgc taaaattacc gttggtcttc cttttgtttc aactgctctt
      781 ttataagctg cataaacttt aacaggatcg tgtccacctc tgtttaattt ccaaatatca
      841 ttgtcactta gattttcaac aagttttgca gtttctggga atttattaaa gaaattttct
      901 ctagtatatg ctccaccttt ttgtttgaag ttttgatact ctccatcaac tgtttgctcc
      961 attagttcaa gaagttttcc tgatgtatct ttttcaagta aattatccca taatccaccc
     1021 caaataactt tgattacttc ccaaccagct cctctaaatt caccttcaag ttcttgaata
     1081 atttttccat ttccacgtac tggaccatca agtctttgta agttacagtt tattacaaaa
     1141 attaaattat ctaactcttc tctagcagcc attccaatag ctcctaaagc ttcaggttca
     1201 tcacactctc catctcccat aaagcagtaa actttttgtc cactacaatc tttaattcct
     1261 ctatttgtaa gatattttaa aaatcttgct tgataaattg cttgtaaagg tccaagaccc
     1321 atagaaactg taggaaattg ccaatatgta ggcataagtt ttgggtgtgg ataagatgat
     1381 aaaccatctg caaaagcttc ttgtctaaag ttatccatct gctcttgagt aaatcttcct
     1441 tctagaaaac ttctagcgta aataccagga cttatatgtc cttgaaaaaa tatcaaatct
     1501 ccaccatctt tttcatttgg tgctttaaaa aaatgattaa aagctacgtc ataaagtgtt
     1561 gctgatgatt gaaacgatgc aatatgacca ccaagttcaa gttgcttgtt tgaagctctt
     1621 tgaaccataa tttgtgcatt ccatctaata atagatctaa tttttctctc taaatccata
     1681 tttgctggaa tttttggttc ctcctcttta ggaatagagt ttatatatgc agttgttgcc
     1741 ttaaaaggta aatgtgcacc acttcttctt gatttatcta ttaatttttc tagtagaaaa
     1801 tgtgctctct caacaccctc ttcatctata acagcttcta aagcctccat ccactcttta
     1861 gtttctagcg gattaacatc ttctaattgt acttttggca taaatatcct ttataaaagt
     1921 tttcttaagt tttaaaacta atttggaatt ttaatttaaa ataaattata attcgcttat
     1981 atcaaatatt ttaaggatta aaatagatga aaattaacaa taaatttaga ttgattataa
     2041 cacaaaatct tagttctaag attaattcaa atatagataa agctttattt aaagctttca
     2101 aaaatgatag ttttccaatc ttaagactct attcttggga ggattgtatt acttttggag
     2161 cagggcaaaa tattgatgat tacaaacaat tacaaagcga ttacaaaaat aatgtttcaa
     2221 aaagattgac aggaggagga gtactttttc atggacacga tatttcatat acaattttat
     2281 taaatcca
//
LOCUS       DAOQAN010000152         2285 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_68703_length_2285_cov_1.765471, whole genome shotgun sequence.
ACCESSION   DAOQAN010000152 DAOQAN010000000
VERSION     DAOQAN010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68703_length_2285_cov_1.765471"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1263)
                     /locus_tag="PKO10_03210"
     CDS             complement(<1..1263)
                     /locus_tag="PKO10_03210"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HNW66191.1"
                     /translation="MQIEILKRDGKKEKFEAFKIEDAIKKAFKSVNTIYDKSIYFSVL
                     FEIKSKNLKAVEDIQDLIEKELFKSEYFDVMKSFMLYRHLHKIQREHILGLTNDTTYV
                     NSTQTIQEYINGEDWRIKANSNTGYSHAGLINNSAGKLIANYWLDKIYTKEQGYAHRN
                     ADIHIHDLDCLSAYCAGWSLRVLLDEGFNEVRGRVESKAPNHFREALGQMANFLGILQ
                     SEWAGAQAFSSFDTYLAPYVFKDKLEFKEIKKAIRSFVYNLNVPARWGQSPFTNITID
                     WTVPSDLKEQIPTRNQEHLFKDLKDDELLKEAKNRGVSTFEELTYKHFQKEMNQINKA
                     YYEIMTQGDKTGQPFTFPIPTVNITEDFDWDGENTDILFENTAKIGSSYFQNFIGSQY
                     KKDKSGNKIPNEEAYKPGHVRSMCCRLQL"
     gene            1435..2256
                     /gene="argF"
                     /locus_tag="PKO10_03215"
     CDS             1435..2256
                     /gene="argF"
                     /locus_tag="PKO10_03215"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HNW66192.1"
                     /translation="MRHFLSLLDFSKDEILEILQLSKQIKDETKKRVFKEYMPKKVLG
                     MIFEKSSTRTRVSFETGIYQLGGVGLFLSSNDIQLGRGEPMKDTARVISRMVDMVMIR
                     TFEQEKLEEFASYSKVPVINGLTDKYHPIQLIADYLTIMEEGLDKNLVVAYIGDGNNM
                     AHSWLNLAAKLGFELRIATPKDYQVDSKVLQTALEEAKKSGAKITTSFDPKEAIKDST
                     VVTTDTWISMGQESEKEKRVKDFSGYMVDEDMMKLAKENAIFLHCLPAYRDYEVS"
BASE COUNT          766 a          348 c          323 g          848 t
ORIGIN      
        1 aagttgaagt ctgcaacaca tacttcttac atgtcctggt ttatatgcct cttcatttgg
       61 tattttattt cccgatttat cttttttata ttggcttcct ataaaatttt gaaaatagct
      121 acttcctatt tttgctgtat tttcaaataa aatatctgta ttttctccat cccagtcaaa
      181 atcttcagtt atatttacag ttggaatcgg aaaagtaaat ggctgacccg ttttatcccc
      241 ttgtgtcata atctcgtagt aagctttgtt tatttgattc atctcttttt gaaaatgttt
      301 atatgtaagc tcttcaaaag ttgaaactcc cctatttttt gcctctttta aaagctcatc
      361 atcttttaaa tctttaaata gatgctcttg atttcgtgtt ggtatttgct cttttaaatc
      421 acttggaacc gtccaatcga tagtaatatt tgtaaatgga ctttgtcccc atcgtgcagg
      481 aacatttaag ttataaacaa agcttcttat agcttttttt atctctttga attcaagttt
      541 atctttaaaa acataaggtg ctaaatatgt atcaaaagag ctaaaagctt gagctcctgc
      601 ccactcactt tgcagtattc ctaaaaaatt tgccatctgt cccaaagctt ctctaaaatg
      661 atttggagcc ttactttcaa ctcttcctct aacttcatta aatccctcat caagtaaaac
      721 tctcaaactc catccagcac aataagcact taaacaatct aaatcatgaa tatgaatatc
      781 tgcatttcta tgagcatatc cttgctcttt tgtatatatt ttatcaagcc aatagtttgc
      841 tattaatttt ccagcactat tgtttataag tccagcatgt gaatatcctg tatttgaatt
      901 tgcttttatt ctccaatctt caccatttat atactcttgt attgtttgag tcgagtttac
      961 ataagttgta tcattcgtta atcctaaaat atgctctctt tggattttat gaagatgtct
     1021 ataaagcata aaacttttca taacatcaaa atattcactt ttgaaaagct ctttttctat
     1081 taaatcttga atatcttcaa cagcttttag atttttagat tttatctcaa ataaaactga
     1141 aaaatatatt gatttatcat aaatagtatt tacactttta aatgcttttt ttatagcatc
     1201 ttcaatttta aaagcttcaa atttctcttt tttaccatct ctttttaaaa tctctatttg
     1261 cataacttat tcctttttta taatcactgg aagtttaaaa gagtttttat taatagctgt
     1321 taagtaaaaa agtcacaaaa gtgttatttt aaggatatta gcttattttc tttaagtgtt
     1381 tgtaaagata aattaactaa aatattggct tttaatccat ataaggaaaa tattatgaga
     1441 cacttcttaa gtttattaga cttctcaaaa gatgaaattt tagaaattct tcaattatca
     1501 aaacagatta aagatgaaac aaaaaaaaga gtttttaaag agtatatgcc taaaaaagtt
     1561 ttaggtatga tttttgaaaa aagctctaca agaactagag tatcttttga aactggtata
     1621 tatcaactag gtggtgtggg gctttttctt tcatcaaatg atattcagct tggtcgtggt
     1681 gaacctatga aagatactgc tagagtaata tctagaatgg ttgatatggt gatgattaga
     1741 acttttgagc aagaaaaact tgaagagttt gcttcatact caaaagttcc tgttataaat
     1801 ggacttacag ataaatatca tcctatacag ttaatcgctg actatcttac aattatggaa
     1861 gaaggacttg ataaaaattt agttgtagca tatattggag atggaaacaa tatggcacac
     1921 tcatggctaa atttagcagc aaaacttgga tttgagctta gaattgctac tccaaaagat
     1981 tatcaagtag atagcaaagt tttacaaact gctttagaag aagctaaaaa aagtggtgca
     2041 aaaattacaa cttcttttga ccctaaagag gctataaaag actcaactgt tgttacaact
     2101 gatacttgga tatctatggg acaagagtca gaaaaagaga aaagagtaaa agatttttct
     2161 ggatatatgg ttgatgaaga tatgatgaaa ttagcaaaag aaaatgcaat atttttacac
     2221 tgtttacctg catatagaga ttatgaagtt agttaagctg tattagaagg aagccaaagt
     2281 agaat
//
LOCUS       DAOQAN010000153         2281 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_68881_length_2281_cov_3.214735, whole genome shotgun sequence.
ACCESSION   DAOQAN010000153 DAOQAN010000000
VERSION     DAOQAN010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68881_length_2281_cov_3.214735"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            142..>2281
                     /gene="uvrA"
                     /locus_tag="PKO10_03220"
     CDS             142..>2281
                     /gene="uvrA"
                     /locus_tag="PKO10_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011990646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNW66193.1"
                     /translation="MSDTIKIFNAKENNLKNINLEIPKNKLIVFTGLSGSGKSTLAFD
                     TLYAEGQRRYIESLSSYARQFLDKVGKPDVERIEGLTPAIAIDQKTTSKNPRSTVGTI
                     TEVYDYFRLLYARVGEQHCHQCNKPISKMSATDVINQVLSLPNEAKLIILAPLINRKK
                     GSFADLLENLRSKGYVRAMIDGVMVRLDEDIELSKTQMHTIKVVIDRVTANETNKDRI
                     AQDVEKGLKESFGELEIEIINHEELGVEKNIHYSEHMACFDCKISFEPLEPISFSFNS
                     PKGACSSCDGLGIRYALDMKKVIDEDLAIEDGAIKIIYGFNKGFYFKMLIAFCEQSQI
                     NIKIPFRDLEEHQKKSILHGTVDEVKFFWKKHKLTRKWDGVVKLAYDMIKDEKEMAEF
                     MTEKKCDSCNGNRLKPASQTVYVAKKTIPEILNIPIEEAHHFFQDENNFSYLSEQNKM
                     IAAPILKEIKERIFFLYDVGLGYITLGRDARTISGGEAQRIRVASQIGSGLTGVMYVL
                     DEPSIGLHERDTSKLIKTLRALQEKGNTVIVVEHDKETIMASDFIVDIGPNAGKFGGE
                     VVFAGTLEKMKKAKTLTALYVTEAKKVEYPHNRKQEDYIEIKNVNINNIKNLDVKLPL
                     RNLVSITGVSGSGKSSLILQTLLPVAQELLNRARKVKKVDGVEIDGLEKLDKVIYLDQ
                     SPIGRTPRSNPATYTGLMDEIRDLFTKTKEA"
BASE COUNT          944 a          281 c          403 g          653 t
ORIGIN      
        1 ttgtgtaaaa aatctagata tagattactc tcaaggtttt tttatagatg agcctaaaga
       61 gttggcttaa tagtaaaata caatactaac tattaacaaa ctttatatat aataaacact
      121 ttaaattttt aaaggcaaat tatgagtgat acaataaaaa tatttaatgc aaaagagaat
      181 aatctaaaaa atataaactt agagatacca aaaaataagc taatagtttt tacaggactt
      241 agtggaagtg gtaaatctac actagctttt gatacacttt atgcagaagg acaaagaaga
      301 tatatagagt ctttatcctc ttatgctaga caatttttag acaaagttgg aaagcctgat
      361 gttgaaagaa tagaaggatt aactccagca attgcgatag atcaaaaaac tacttcaaaa
      421 aacccaagat caactgtagg aacaataact gaagtatatg actactttag acttttatat
      481 gcaagagttg gagaacaaca ttgtcatcaa tgcaataaac caatttcaaa aatgagtgct
      541 acggatgtaa taaatcaggt attatcgcta ccaaatgaag caaaacttat aatactagca
      601 cctttaataa atagaaaaaa aggaagtttc gcagatttac ttgagaatct tcgatcaaaa
      661 ggttatgtaa gagctatgat agatggagta atggtaagac ttgacgagga tatagagtta
      721 tccaaaactc aaatgcatac aataaaagta gtaatagata gagttacagc aaatgaaaca
      781 aacaaagata gaattgctca agatgttgaa aaaggtctaa aagagagttt tggagagcta
      841 gaaattgaga taataaatca cgaagagctt ggagttgaga aaaacattca ctactcagaa
      901 catatggctt gttttgattg taaaatatca tttgaaccac tagaaccaat atctttctct
      961 tttaactctc caaaaggggc ttgtagctct tgcgatggat taggaataag atatgcactt
     1021 gatatgaaaa aggttattga tgaagattta gctattgaag atggagcaat aaagataata
     1081 tatggattta acaaaggttt ctattttaaa atgctaatag ctttttgtga acaatcacaa
     1141 ataaatataa aaattccttt tagagactta gaagaacatc aaaaaaaatc tatcttgcac
     1201 ggaactgttg atgaagtaaa attcttttgg aaaaaacaca aactcacaag aaaatgggat
     1261 ggtgttgtaa aattagccta tgatatgata aaagatgaaa aagagatggc tgaatttatg
     1321 acagaaaaaa aatgtgactc ttgcaatgga aatagactaa aaccagctag tcaaactgta
     1381 tatgtggcta aaaaaactat tcctgaaatt ttaaatattc caatagaaga agctcaccat
     1441 ttttttcaag atgaaaacaa tttttcatat ttaagtgaac aaaataagat gattgcagct
     1501 ccaattttaa aagagataaa agagagaatt ttctttttat atgatgttgg tttaggatat
     1561 attactttag gaagagatgc aagaacaata agtggtggag aagcccaaag aataagagtt
     1621 gcttcacaaa ttggaagtgg tttaactggg gttatgtatg tactagatga accatctatt
     1681 ggacttcatg aaagagatac tagtaaacta ataaagacat taagagcgct acaagaaaaa
     1741 ggaaatactg taattgtagt tgaacatgat aaagagacaa ttatggcttc tgattttatt
     1801 gttgatattg gaccaaatgc tggaaaattt ggtggtgaag tagtatttgc tggaacttta
     1861 gaaaaaatga aaaaagcaaa aactctaact gctttatatg taacggaagc aaaaaaagtt
     1921 gaatatcctc ataatagaaa acaagaagat tatatagaga taaaaaatgt aaatataaac
     1981 aatatcaaaa atctagatgt aaaacttcct cttagaaatc tagtaagtat tacaggagtt
     2041 agtggaagtg gaaaatcttc acttattctt caaacgctac ttcctgttgc acaagagctt
     2101 ttaaatcgtg ctaggaaagt aaaaaaagtt gatggagtag aaatagatgg tttagaaaaa
     2161 ctagataaag ttatatatct tgaccaaagc ccaattggtc gtactcctag aagtaatcca
     2221 gcaacttata caggacttat ggacgaaata agagaccttt ttactaaaac aaaagaagct
     2281 a
//
LOCUS       DAOQAN010000154         2280 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_68955_length_2280_cov_2.783820, whole genome shotgun sequence.
ACCESSION   DAOQAN010000154 DAOQAN010000000
VERSION     DAOQAN010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68955_length_2280_cov_2.783820"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            124..441
                     /locus_tag="PKO10_03225"
     CDS             124..441
                     /locus_tag="PKO10_03225"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNW66194.1"
                     /translation="MAIKNVLKNAIQFSFENSKVIVKIFEKDNLLNISIKDFGIGIPL
                     NEQEKIFDKFYRTDKSRNKNSGGTGLGMSILKKIVDIHKGEITIKSEENKETTVTLSF
                     NNK"
     gene            534..1274
                     /locus_tag="PKO10_03230"
     CDS             534..1274
                     /locus_tag="PKO10_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNW66195.1"
                     /translation="MNLIQIENLSYSYSNTKALENINLEIKSDDFLAIIGPNGGGKST
                     LFKLILELLPLQSGRLIKNIKNSEVGYVPQNTNLNIDFPITSLEVVLMGHISSKKRLF
                     GYSKDEISCALESLNQVGMKDYANKKIGDLSGGQRQRVFIARALCSNPKIMLLDEPTA
                     SIDVQGQQEIYELLRELNKSICIVVVSHDLSILLNYAKNVAHINRSLVYHSLAEVQKN
                     VTLADDHLCEVELLSALGKTQICCNHVH"
     gene            1290..2090
                     /locus_tag="PKO10_03235"
     CDS             1290..2090
                     /locus_tag="PKO10_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HNW66196.1"
                     /translation="MFEILQYDFIQNALIAGILISIAAGIIGTLVVVNKITFLTGGIA
                     HSAYGGIGIAIFLGIPVLFGATIFSVLTAIIIAIITLKNRSRIDAIIGMMWASGMAIG
                     IIFVDLTPGYNVDLMSYLFGSIIAVSSEDIIYMTLLDIFIIAIVVYFYKEILSVSYDS
                     EFASLRGINVKFFYTLILILSALCVVAAIKAVGLILVIALLTIPTYLAETFATKLSSM
                     MIISSILATMFTISGLVVSYFYDISSGASIIMVAVVALGVVKLIKYKK"
     gene            2113..>2280
                     /locus_tag="PKO10_03240"
     CDS             2113..>2280
                     /locus_tag="PKO10_03240"
                     /inference="COORDINATES: protein motif:HMM:NF024899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HNW66197.1"
                     /translation="MKKIDEKIKQGVVNTLRVNRVSEPGIYLISGDETEVLLPNAYVK
                     KDMAIDSLLDVF"
BASE COUNT          863 a          232 c          381 g          804 t
ORIGIN      
        1 ttgtcctatt ttttataaaa aatatagaca ggttacaagg ttatgtaaat ggtgtcaaac
       61 gaagtgctaa actaggcagt tgaagataat ttagagtttt ttggtttttc aaatttatta
      121 aaaattgcaa taaaaaatgt tttaaaaaat gctattcaat ttagttttga aaattcaaaa
      181 gttatagtaa aaatatttga aaaagataat ttactaaaca tttcaataaa agattttgga
      241 ataggaattc cactaaatga acaagaaaaa atatttgata aattttatag aacagataaa
      301 agtagaaata aaaactctgg aggaactggt cttggtatgt cgattttaaa aaaaatagta
      361 gatattcaca aaggagaaat aactataaaa agtgaagaga ataaagagac aacagtaact
      421 ttatctttta ataataaata aagaactact ctttttcaat attaaaattt atatcaatag
      481 aaattaattc atatttagat aatattgcaa ccaagttgca aacaaaggtt aatatgaatt
      541 taatacaaat agagaattta tcttactcat attctaatac aaaagctttg gaaaatataa
      601 atcttgaaat taaaagtgat gattttttag ctattattgg accaaatggt ggtggaaaat
      661 ctactctttt taaacttatt ttagagttac ttcctcttca aagtggaaga ttaataaaaa
      721 atataaaaaa tagtgaagtt ggttatgtcc cacaaaatac aaatttaaat attgattttc
      781 caattacttc tttagaagta gttttaatgg gtcatataag ttctaaaaaa agattatttg
      841 gttactcaaa agatgaaatc tcttgtgctt tagagtcttt aaatcaagtt gggatgaaag
      901 attatgcaaa taaaaagata ggtgatttaa gtggaggtca aaggcaaaga gtttttatag
      961 cacgtgcact ttgttcaaat ccaaaaatta tgcttttgga tgagccaact gcaagtattg
     1021 atgtgcaagg acaacaagag atttatgaac ttttaagaga gttaaataaa tctatttgta
     1081 ttgttgttgt aagtcatgat ttatcaattc ttttaaatta tgcaaaaaat gtagctcaca
     1141 taaatagaag tttggtttat cacagccttg ctgaggttca aaaaaatgtt actttagcgg
     1201 atgatcattt gtgtgaagta gaacttttat cagcattggg taaaacacaa atttgctgta
     1261 accatgtaca ttaaaaaggt aaaaatataa tgtttgaaat tttacaatat gattttattc
     1321 aaaatgcttt gatagctgga attttaatct caatagcagc tggaataatt ggaactttgg
     1381 ttgtggtaaa taagattact tttttaacag gtggaatagc tcacagcgct tatggaggaa
     1441 taggaatagc tatattttta ggaatcccag ttttatttgg agcgacaatt ttttcagttc
     1501 taactgcaat aataatcgct attattactt tgaaaaatag aagcagaata gatgcaatca
     1561 ttgggatgat gtgggcaagt ggaatggcta ttggaattat ttttgtggat ttaactccag
     1621 gatacaatgt agatttaatg agctatttgt ttggaagtat tatcgctgtt tcaagtgaag
     1681 atattattta tatgactctt ttagatatct ttattattgc aattgttgtc tatttttata
     1741 aagagatttt atctgtatct tatgatagtg aatttgcaag tttaagaggt ataaatgtaa
     1801 agttttttta tacactaatt cttatattat cagcactttg cgtagttgct gctataaaag
     1861 cagttggact tattttagta attgcacttc ttacaattcc aacatattta gctgaaactt
     1921 ttgctacaaa actatcttct atgatgatta ttagttcaat tttagctaca atgtttacaa
     1981 tatctggtct tgtagtctca tatttttatg atataagttc gggagctagt ataataatgg
     2041 tcgcagttgt tgctttagga gttgtaaagc taataaaata taaaaaataa aaaagaaaga
     2101 acggaaaaac caatgaaaaa aatagatgaa aaaataaaac aaggtgttgt aaacacttta
     2161 agagtaaata gagttagtga acctggaatt tatctgatta gtggagatga aacggaagtt
     2221 ttattaccaa atgcttatgt aaaaaaggat atggcaattg atagtttact tgatgtattt
//
LOCUS       DAOQAN010000155         2265 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_69685_length_2265_cov_2.237557, whole genome shotgun sequence.
ACCESSION   DAOQAN010000155 DAOQAN010000000
VERSION     DAOQAN010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69685_length_2265_cov_2.237557"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="PKO10_03245"
     CDS             <1..256
                     /locus_tag="PKO10_03245"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNW66198.1"
                     /translation="LIVNEEFRGLGLGKKLVEKCVEEAKFYGIKQVLSLTYEKDFFNS
                     CGFREIPKEDIPEQKIWADCIKCKLFPICNEIAMVKDIEF"
     gene            complement(253..1197)
                     /locus_tag="PKO10_03250"
     CDS             complement(253..1197)
                     /locus_tag="PKO10_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66199.1"
                     /translation="MILKKIIIKDQKELYRHKNYLLGLDLEFNSTKKEYSNSSEINFD
                     NLFELTEFLKNHNFTYSIVEEKITDFKKQILAKYKTLQIDNNNIFIVEKNSENKIYLL
                     NQIKNNINIVDLKKSNMKMYKIPKNSLENSNLSIKVLEILASNKGDFEELFDIFAILE
                     NQDSQSILYLEKLKKFKYFCISKINEQQKDMFLCNCVPNFFPETNFYIKGNRVFSDYT
                     QYFLNYEQEIKIWKYLYSNKDLVGVYKEPSLYELFVGRKIYIFDEFKNRVKVIIKNAQ
                     YLENKGISITLSNGVSSQKISQIFTKEELLKRVIEARD"
     gene            complement(1201..1473)
                     /locus_tag="PKO10_03255"
     CDS             complement(1201..1473)
                     /locus_tag="PKO10_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014473962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HNW66200.1"
                     /translation="MQEDNQKVQKAVALQYDKNTNNAPKITAKGKGETANNIIKIAKD
                     NKIPIKKDEDLVELLSKIDIDKEIPTSMYKAVAEIFAFIYELSNKR"
     gene            complement(1477..>2265)
                     /locus_tag="PKO10_03260"
     CDS             complement(1477..>2265)
                     /locus_tag="PKO10_03260"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HNW66201.1"
                     /translation="ISLPDIFSKSEAILNSVQNSNISNFSNNFATNLNPLLTALKESL
                     QAINPKNIEIQNQINSLIKKVENIIQEYTNNQLDNQKDNQKLDNDFKSILLKMQDEVA
                     QKTDIKSQDSLKTINNLLTQIDMQQLTSLVSNSNFVYIPFFWEMLEDGTVEIKEKEED
                     KFFCQIKLTLKDFGKIDLMLSMYDENKLDMTIYAQREHFKVTLRDNLQKLKLALNEAN
                     IIPMHIKLLDMKEESENKEQKPTNVYQNNYNNDFITSSRIDIKA"
BASE COUNT          714 a          285 c          267 g          999 t
ORIGIN      
        1 cttgatagtc aatgaagagt ttagaggttt aggcttaggc aaaaaacttg tagaaaagtg
       61 tgttgaagag gcaaaatttt atggaataaa acaggtttta tctcttactt atgaaaaaga
      121 tttttttaat tcatgtggat ttagagagat tccaaaagaa gatatacctg agcaaaaaat
      181 ttgggctgat tgtattaagt gtaaactatt cccaatttgt aatgaaatag ccatggtaaa
      241 ggatatagag ttttaatctc tagcttctat tactcttttt agaagctctt cttttgtaaa
      301 tatttgagat attttttgac tagatactcc atttgatagt gttatactaa ttcctttatt
      361 ttctaaatat tgtgcatttt ttattataac ttttactcta tttttaaatt catcaaaaat
      421 ataaattttt ctcccaacaa aaagttcata taaactaggc tctttgtaaa ctcctactaa
      481 atctttattt gaatataaat atttccaaat tttaatctct tgttcgtagt ttagaaaata
      541 ttgcgtatag tcagaaaaaa ctctatttcc ttttatataa aagtttgttt cagggaaaaa
      601 atttggtacg cagttgcata aaaacatatc tttttgctgt tcatttatct ttgaaataca
      661 aaaatattta aattttttta atttttctaa gtaaagaata gattgtgaat cttgattttc
      721 aagtattgca aaaatatcaa agagttcttc aaaatcacct ttatttgaag ccaatatttc
      781 aagaactttt atacttaaat ttgagttttc caaactattt tttggaattt tatacatctt
      841 catatttgac ttttttaaat caactatatt tatattgttt tttatctgat taagaagata
      901 tattttattt tcactatttt tttctacaat aaaaatgttg ttattatcaa tctgaagtgt
      961 cttgtatttt gccaaaatct gctttttaaa atcagttatt ttttcttcaa ctatactgta
     1021 tgtaaagtta tgatttttta aaaactcagt tagttcaaat agattatcaa aatttatttc
     1081 gctactattt gaatactctt tttttgtact attaaattct aaatctaatc caagaagata
     1141 gtttttatgc ctatataact ctttttggtc ttttattata atctttttta aaatcattct
     1201 ttaccttttg tttgataatt cataaataaa tgcaaaaatt tctgcaacag ctttatacat
     1261 cgaagttggt atctccttat ctatatctat tttagataat aattcaacta aatcctcatc
     1321 tttttttata ggaattttgt tatcttttgc aatttttatt atgttatttg ctgtttcacc
     1381 ttttcctttt gcagttattt taggagcatt atttgtattt ttatcatatt gtaaagctac
     1441 tgctttttgt actttttgat tatcttcttg catctattat gcctttatat caattcgtga
     1501 gctagttata aaatcgttgt tataattatt ttgataaaca tttgttggtt tttgctcttt
     1561 attttcactc tcttctttca tatcaagaag ttttatatgc ataggtataa tatttgcttc
     1621 atttagagcc aattttaatt tttgcaaatt atctcttagt gtaactttaa aatgctctct
     1681 ttgcgcataa atagtcatat caagtttatt ttcatcatac attgaaagca ttaaatcaat
     1741 ttttccaaaa tcttttagag taagtttaat ttggcaaaaa aacttatctt cctctttctc
     1801 tttaatctca actgtaccat cttctaacat ctcccaaaaa aatggaatat atacaaaatt
     1861 agagtttgaa acaagtgaag ttagttgttg catatcaatt tgagttaaaa gattgtttat
     1921 cgtttttaag ctatcttgag actttatatc tgttttttgt gcaacttcat cttgcatttt
     1981 aagaaggatt gatttgaaat cattatcaag cttttgatta tctttttgat tgtctaactg
     2041 attatttgta tattcttgaa taatattttc tacttttttt atcaaagagt ttatctgatt
     2101 ttgtatctct atattttttg gattaatagc ttgtagactc tcttttaacg cagttaaaag
     2161 tggatttaaa tttgtagcaa aattatttga aaaatttgag atattactgt tttgtacact
     2221 atttaaaata gcttcacttt ttgaaaatat gtctggtagt gaaat
//
LOCUS       DAOQAN010000156         2260 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_69908_length_2260_cov_2.814512, whole genome shotgun sequence.
ACCESSION   DAOQAN010000156 DAOQAN010000000
VERSION     DAOQAN010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69908_length_2260_cov_2.814512"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="PKO10_03265"
     CDS             <1..421
                     /locus_tag="PKO10_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014467949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HNW66202.1"
                     /translation="LDLTNYSKKEEILNLAKQRANTLLELKKSILDIKNSPNEYEEAG
                     VKKFVKDDTNSILKEYLELLEENKNSFVSPSELENITKPFIETKGLKFPQLFQPIRIA
                     LTGGTQAPSVYDILFILDFAESKIRISKALEKNFKNS"
     gene            474..755
                     /locus_tag="PKO10_03270"
     CDS             474..755
                     /locus_tag="PKO10_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014770658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HNW66203.1"
                     /translation="MNIYVGNLSYKMNDNDLEAVFAKFGAVKSAKIIMDKETGRSKGF
                     AFVEMEEASAGNAAIEALNGKETDGRTLRVNEAKPKEDRPRDSRPRRNF"
     gene            797..1453
                     /locus_tag="PKO10_03275"
     CDS             797..1453
                     /locus_tag="PKO10_03275"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNW66204.1"
                     /translation="MKILLLEDDLILNEILEEHLIKQKHQITTTFSSNEAIKELYSET
                     FDLLLLDVNVPDLNGFELLKELRDNNILTPTIFITSLNMVEDMQKGFDSGCDDYIKKP
                     FELKELDIRINNIKRLFNIDIKIIKIDENSYLDLQNLEINLNKKSFIISKKESEILAY
                     LLNNPKKTISIEEIINNIWSYEDSVESSTIRTYIKNLRKILGEDKILNIRGVGYRFNF
                     "
     gene            1431..>2260
                     /locus_tag="PKO10_03280"
     CDS             1431..>2260
                     /locus_tag="PKO10_03280"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HNW66205.1"
                     /translation="MDIDLTSSEKKSFFSFLALYLGSSFILMLIALFFYYQNEKTLYL
                     DLVKSNMQNIVSKVSNEIIISHMLDVEFDKNIYLNNQDYKISFYDKDKNLLFGNLDEK
                     LNFEQNFYNDEEKLIIVDSSTVGHLGIWYIALKDNSLKEKISNLKLNIFLIFLIFYTI
                     IAIVSWSLAKLFLKPIKNERERLNNFIKDTTHELNTPISAIIMSCEDDNLTKKQLDRI
                     KFSAKRVSEIYKDLTYIFLGNIEKKSLDKIDLSKVIKEEIINFEPMIARKRLKININI
                     "
BASE COUNT          976 a          218 c          329 g          737 t
ORIGIN      
        1 tttagattta actaattact ctaaaaaaga ggagatttta aaccttgcaa aacaaagagc
       61 aaatacacta ttagaattaa aaaaatctat acttgatata aaaaatagtc ctaatgagta
      121 tgaagaagct ggggttaaaa aatttgtaaa agatgataca aattcaattt taaaagagta
      181 tttagagctt ttagaagaga ataaaaatag ttttgtatct cctagtgaac ttgaaaatat
      241 tacaaaacca tttatagaaa caaaaggctt gaaatttcca caactgttcc aacctataag
      301 aattgcatta acgggtggaa cacaagcacc atcagtttac gatatacttt ttatcttaga
      361 tttcgctgaa agtaagataa gaatttcaaa agctttggaa aaaaatttta aaaactcttg
      421 atttaagaaa aatattaagg cataatgtct aactttttca aaggataaga attatgaaca
      481 tttacgttgg gaatttatcg tacaaaatga acgataatga tttagaagca gtttttgcta
      541 aatttggtgc agttaagagc gcaaaaataa ttatggacaa agaaacagga agatctaaag
      601 gttttgcttt tgttgaaatg gaagaagcta gtgcaggaaa tgctgctatt gaagctttaa
      661 atggtaaaga gactgatggt agaactttaa gagtaaacga agctaaacca aaagaagata
      721 gaccaagaga tagcagacca agaagaaatt tctaagactg caaagggggt ttaaaaaccc
      781 ctttttttat gccaaaatga aaattctact tctagaagat gatttaatct taaatgaaat
      841 tcttgaagaa cacttaataa aacaaaaaca tcaaattact acaacttttt cttcaaatga
      901 agctataaaa gagctatatt cagagacttt tgatttactt ctattagatg taaatgtacc
      961 agatttaaat ggctttgaac tattaaaaga gctaagagat aataatattt taactccaac
     1021 aatatttata acatctctaa atatggtcga agatatgcaa aaagggtttg atagtggatg
     1081 tgatgactat attaaaaaac cttttgagtt aaaagagctt gatattagaa taaataatat
     1141 aaaaaggctt tttaatatag atataaaaat tataaaaata gatgaaaata gttatttaga
     1201 tttacaaaac ttggagataa atttaaataa aaaaagtttt atcatctcaa aaaaagagtc
     1261 tgaaattctt gcatatttat taaataatcc aaaaaaaact atcagtattg aagagattat
     1321 aaataatatt tggtcatatg aagatagtgt agaaagctct acaattagaa cttatataaa
     1381 aaacctgcga aaaatattag gtgaagataa aatattaaat ataagaggag ttggatatag
     1441 atttaacttc tagtgaaaag aaaagttttt ttagcttttt agctctttat ttaggatcct
     1501 cttttatatt aatgttaatt gccctatttt tctattatca aaatgagaaa acactttatt
     1561 tggatttagt aaaatcaaat atgcaaaata tagtatctaa agtttcaaat gaaataataa
     1621 tatctcatat gttggatgtc gaatttgata aaaatatata tttgaataat caagattata
     1681 aaatctcttt ttatgataaa gataaaaatt tattatttgg aaatttagac gaaaagttaa
     1741 attttgaaca aaatttttat aatgatgaag aaaaattaat tattgttgat agttcaactg
     1801 taggacattt aggaatttgg tatatagctt taaaagacaa tagcttaaaa gagaaaatat
     1861 caaatttaaa attaaatatt tttcttattt ttttaatatt ttatacaata attgctattg
     1921 ttagctggtc tttagcaaaa ctatttctaa aaccaataaa aaatgagaga gaaagattaa
     1981 ataactttat aaaagatact actcatgagt taaatactcc aataagtgca ataattatgt
     2041 cttgtgaaga tgataattta actaaaaaac aactagatag aataaaattt agtgcaaaaa
     2101 gagttagtga gatttataaa gatttgacgt atatattttt aggaaatatt gaaaaaaaaa
     2161 gtttagataa aatagattta tcaaaagtta ttaaagaaga gattataaat tttgagccaa
     2221 tgattgctag aaaaagatta aaaataaaca ttaatataga
//
LOCUS       DAOQAN010000157         2254 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_70230_length_2254_cov_2.241473, whole genome shotgun sequence.
ACCESSION   DAOQAN010000157 DAOQAN010000000
VERSION     DAOQAN010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70230_length_2254_cov_2.241473"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            170..1513
                     /gene="murJ"
                     /locus_tag="PKO10_03285"
     CDS             170..1513
                     /gene="murJ"
                     /locus_tag="PKO10_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HNW66206.1"
                     /translation="MNQKSSQKNSQKSRLLKSIFTNSSGILISRITGFIRDLMTASIL
                     GANVYSDIFFIAFKFPNLFRSIFADGAFTQAFIPSYAKSKYKIRFSSIIFLQILVFLI
                     VLSLIVTMFSHLFAKAFAIGFSEETINLAAPLFAINFYYLPLIFVVTFMGALLQYKHH
                     FATTAYSTALLNLSMIASLIIAKGLEQYTITFYLSFGVIFGGILQVIVHIIAIRRANL
                     GKIFIFKNHKKKEENKFYKNFFAATLGSSTMHISAFIDTWLASLLISGSISYLYYANR
                     VFQLPLAIFAIATSIALFPMVAKAIKNKNEDEALKLMKKSALILFAVLTISMVVGIAL
                     DKFIIELLFQRGAFTSEDTINTALILKMYLIGLLPFGLAKIFSLWLYAKEQQVLTAKI
                     SAQSLVANIIFSLILIKPFGAAGLAFSGTLSGFVLFFLTLRAFGFDKFVKMFKNY"
     gene            1567..2037
                     /locus_tag="PKO10_03290"
     CDS             1567..2037
                     /locus_tag="PKO10_03290"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HNW66207.1"
                     /translation="MINFFKQYTPFYKNYKLQIFYAFIGIILVASATAGTAYAIQPLL
                     DDIFINKNVDMLYIMPIIVVVLYTAKGFGGYIQAYFISYIGQDITRIIRDRLFSHVLK
                     LDLDFFQKTHGGELVSRIINDINRIQSAVSIFSRDNKRELNYCCFSCTCNLSFS"
     gene            1952..>2254
                     /locus_tag="PKO10_03295"
     CDS             1952..>2254
                     /locus_tag="PKO10_03295"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HNW66208.1"
                     /translation="MQFRYLAEIIRESLTIVALVALVIYRSPELAFYGLIVLPLAFYP
                     LSLLAKKMKKLSFKSQESNSDITSSLTESFNNIEIIKANNTEILEAKKFSVFNMIFF"
BASE COUNT          799 a          278 c          316 g          861 t
ORIGIN      
        1 aaggtaattt acaaattaat ggtggaaaaa atataaaatt gtttgcaaaa gataaaaata
       61 ttttaccaaa agatggttct aaaatagaga tattaaatgc acaattaggt tcatttagag
      121 gaaatatgca aataattttt catactaaag atgactacaa agagttaaaa tgaaccaaaa
      181 aagtagccaa aagaatagcc aaaaaagtag attgttaaaa tcaattttta caaatagttc
      241 tggtatttta atttcaagaa taacaggctt tataagagat ttaatgacag cttcaatttt
      301 aggggcaaat gtctattctg atatattttt tatagcattt aaatttccaa atttatttcg
      361 tagtatcttt gctgatggtg cttttacaca agcttttatt ccttcttatg caaaatcaaa
      421 atataaaata agattttcat ctattatatt tttacaaatt ttagtttttt taattgtttt
      481 atctttgatt gttactatgt tttcacatct ttttgcaaaa gcttttgcta ttggatttag
      541 tgaagaaaca attaatttag ctgcaccact atttgctata aatttttact atttacctct
      601 tatttttgtg gtaactttta tgggtgcttt acttcaatat aaacaccatt ttgctacaac
      661 tgcatactca actgcacttt taaatctatc aatgatcgct agtttgatta ttgcaaaagg
      721 tcttgagcaa tatacaatta cattttatct atcttttggg gttatatttg gtggaatttt
      781 gcaagtaatt gttcatatta ttgcaattag aagagcaaat ttaggaaaaa tatttatatt
      841 taaaaatcat aagaaaaaag aggaaaataa gttttataaa aacttctttg cagcaacttt
      901 aggctcttca actatgcata tttcagcttt tatagatact tggctagctt cactgttaat
      961 tagtggttct atatcatatt tatactatgc aaatagagtt tttcaactac ctcttgcaat
     1021 ttttgcaata gcaacttcaa tagcactttt tccaatggtt gcaaaggcta taaaaaataa
     1081 aaatgaagat gaagctctaa aacttatgaa aaaatcagct ctgattttat ttgcagtttt
     1141 gacaatttca atggttgtag gaatagctct tgataaattt attatagaac ttctattcca
     1201 aagaggtgct tttacaagtg aagacacaat aaacactgca ctaattttaa aaatgtattt
     1261 aataggactt ttgccttttg gcttagcaaa aatattttca ctttggctat atgcaaaaga
     1321 gcaacaagtg ctaactgcta aaatatctgc tcaaagttta gttgcaaata ttatattctc
     1381 tttaattcta ataaaacctt ttggagcagc tggtttagct ttttcaggaa cactaagtgg
     1441 ttttgtacta ttttttctta ctttaagagc ttttggtttt gataaatttg ttaaaatgtt
     1501 taagaactat tagttttagc aaacatttga taaaattaaa gacttttttt taattttagg
     1561 gaatatatga taaatttttt taaacaatac acaccatttt ataaaaacta taaattacag
     1621 attttttatg cttttattgg aattattcta gttgcaagtg caactgctgg aacagcttat
     1681 gcaattcagc ctcttttaga tgatattttt ataaataaaa atgtagatat gctgtatatt
     1741 atgccaataa tcgtagttgt tttatataca gcaaaaggtt ttggtggata tattcaagca
     1801 tattttatat cttatattgg gcaagatatt acaagaatta taagagatag acttttttca
     1861 catgttttaa aattggactt agattttttc caaaaaacac atggaggaga gcttgtaagt
     1921 agaataataa atgatataaa tagaattcaa agtgcagttt cgatatttag ccgagataat
     1981 aagagagagc ttaactattg ttgctttagt tgcacttgta atttatcgtt ctcctgaact
     2041 tgcattttat ggacttattg ttttaccttt ggctttttat cctttaagtt tactagcaaa
     2101 aaagatgaaa aaactatctt ttaaatctca agagagcaac tcagatatta catcaagtct
     2161 tacagaatcg tttaataata ttgagataat aaaagcaaat aacactgaaa ttttagaagc
     2221 aaaaaaattt agtgtattta atatgatttt cttt
//
LOCUS       DAOQAN010000158         2243 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_70752_length_2243_cov_2.225777, whole genome shotgun sequence.
ACCESSION   DAOQAN010000158 DAOQAN010000000
VERSION     DAOQAN010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70752_length_2243_cov_2.225777"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(157..525)
                     /locus_tag="PKO10_03300"
     CDS             complement(157..525)
                     /locus_tag="PKO10_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNW66209.1"
                     /translation="MAKLLIVDDSTMLRDMLNYALNEGGYTDVIEAVDGVDGLAKAKN
                     TNFDLIITDVNMPNMDGLTLIGELRKIPQYLKTPILVLTTERSDEMKAKGKAAGANGW
                     LVKPFDPKRLIEVVQKVIGS"
     gene            complement(590..>2243)
                     /locus_tag="PKO10_03305"
     CDS             complement(590..>2243)
                     /locus_tag="PKO10_03305"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014474228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HNW66210.1"
                     /translation="KDDLENSTLDLYIAGSKDELLMIEMKTISSLNDSKHFTNEMEEQ
                     TLIEAISFAQEALKKANIAYETNFEKACKVKANVELVEFAIEKDVIDYVRDNFSNDIK
                     DAIKKLAKSERAVELKELAKSIAKNDYCTINEVEFKTIFEAVNIVKRELVRAMIVNEK
                     VRADGRGLKDVRPINIETNILPSAHSSCLFTRGETQALVVGTIAGAKDGQMYEVLTDK
                     STSMENLMVHYNFPGFSVGEAKPIFSVGRRELGHGNLAKKALESTINKNFKDTFRIVS
                     EILESNGSSSMATVCGGSLALKAANIPVSDLVAGVAMGMVVEGDKYSILTDIMGLEDH
                     DGDMDFKVAGTSKGITALQMDIKLGGIELNVLQEALLQAKDGRVHILNIMEEASKDIV
                     SSPALPKIEEFEVDSSKMMVIIGKGGATIKEIIEKYSVNIDLDRDNGIVKVSGENSSK
                     ISEANLFIKDLVNNSTSYKAPVKIDFEKLYSVDEIVSGKVERIVDFGAFILLAKGGEG
                     LLHISKISKQRVEKVSDILSVGQEIDVKVLKIQKDKIELGLN"
BASE COUNT          687 a          438 c          238 g          880 t
ORIGIN      
        1 aaaactgact catatcaatt gtcattgctg tactccattt atatatttac cgctttatcg
       61 caacaattac tctgcaggaa cgatctttta gatcgcgaat aattgtcgct gtaagcctcg
      121 ttgccttcgc gatattgata tcgccctacc aaatttttaa gaaccaatga ccttttgcac
      181 tacttcaatt aggcgttttg ggtcaaacgg tttaactaac caaccattgg cacctgcagc
      241 tttaccctta gctttcattt catcacttct ttcagttgtt aaaactaaaa tgggtgtttt
      301 taaatattga ggaatttttc taagttcacc aattaaggtt aaaccatcca tatttggcat
      361 attaacatct gtaataatta aatcaaaatt tgtatttttt gccttagcta gaccatctac
      421 tccatctact gcttcaataa catctgtata accaccttcg tttaaagcgt agtttaacat
      481 atccctaagc attgtagaat catctactat taaaagttta gccatatatt agccccttga
      541 ttttattcat ttaaaaattt ttcccatcct aacacaaaat taattaatat taatttaaac
      601 ctagttctat tttatctttt tgaattttta gaacttttac atctatttct tgtccaacac
      661 ttagaatatc gcttactttt tctactcttt gttttgaaat tttagatata tgtaaaagac
      721 cttctcctcc ttttgcaaga agaataaatg caccaaaatc aactattctt tcaacttttc
      781 cagaaacaat ttcatctaca ctataaagtt tttcaaaatc tatttttact ggtgctttgt
      841 atgaagttga gttatttact aaatctttta taaaaagatt cgcttctgat attttagaag
      901 agttttctcc acttactttt acaattccat tatctctatc taaatctata tttacagagt
      961 atttttcgat aatctcttta attgtagctc caccttttcc aataataacc atcattttag
     1021 aactatcaac ttcaaactct tcaatttttg gtaaagcagg acttgaaact atatctttac
     1081 ttgcttcttc cataatattt aaaatatgaa ctctaccatc ttttgcttgc aaaagtgctt
     1141 cttgtagaac atttaattca attccaccaa gttttatatc catttgtaaa gctgtaatac
     1201 cttttgaagt tccagcaact ttaaaatcca tatctccatc atggtcttca agtcccataa
     1261 tatcagttaa aatagaatat ttatcacctt caacaaccat tcccatagca actcctgcta
     1321 caagatctga aactggaata ttcgcagctt taagtgccaa tgaaccacca caaacagttg
     1381 ccatagaaga agaaccgttt gattctaaaa tttctgaaac tattctaaaa gtatctttga
     1441 agttcttatt tatcgtagat tctaatgctt tttttgctag atttccgtga cctaactctc
     1501 ttcttcctac gctaaaaatt ggttttgctt caccaacact aaatccaggg aaattataat
     1561 gaaccataag attttccata gatgttgatt tgtctgttaa aacttcatac atttgtccat
     1621 ctttcgctcc agcaatcgtt cctacaacta aagcttgtgt ttcacctctt gtaaaaagac
     1681 aagaagagtg agctgatggt aaaatatttg tctcaatatt tattggtctt acatctttaa
     1741 gacctcttcc atctgctctt actttttcat ttactatcat agctctaact aattctcgtt
     1801 ttacaatatt aactgcttca aaaattgttt taaattcaac ttcatttata gtacaataat
     1861 cattttttgc tatacttttt gccaactctt ttaactcaac agctctttca ctttttgcta
     1921 attttttgat agcatctttt atatcatttg agaaattatc tcttacataa tcaattacat
     1981 ctttttctat tgcaaactca actaattcta cattagcctt tactttacaa gctttttcaa
     2041 aatttgtttc ataagctata tttgcttttt ttaatgcctc ttgtgcaaaa gatattgctt
     2101 ctattaaagt ttgttcttcc atctcgtttg taaaatgttt agaatcattt aaagaagaga
     2161 ttgttttcat ctcaatcata agtaattcat cttttgaacc agctatatat aaatctaaag
     2221 tagaattttc taaatcatct ttt
//
LOCUS       DAOQAN010000159         2240 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_70887_length_2240_cov_3.293822, whole genome shotgun sequence.
ACCESSION   DAOQAN010000159 DAOQAN010000000
VERSION     DAOQAN010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70887_length_2240_cov_3.293822"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(93..482)
                     /gene="rpsI"
                     /locus_tag="PKO10_03310"
     CDS             complement(93..482)
                     /gene="rpsI"
                     /locus_tag="PKO10_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011139914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HNW66211.1"
                     /translation="MAKVYATGRRKTAIAKVWLENGNGQLTINGQSLDQWLGGHDSIK
                     KRVMQPLHVSKQESSVNIIVKTLGGGYSAQADAARHGISRALVAFDEQFRTILKPHGL
                     LTRDARSVERKKYGKKKARKSSQFSKR"
     gene            complement(488..907)
                     /gene="rplM"
                     /locus_tag="PKO10_03315"
     CDS             complement(488..907)
                     /gene="rplM"
                     /locus_tag="PKO10_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HNW66212.1"
                     /translation="MKFTQMAKANEIERSWVVVDAEGKVFGRIITEVATILRGKNKPC
                     FTPNVDCGDYVVIINASKAKFTGAKLEVKNYYTHSGYFGSTKTHKMSDMIEKNPEKLF
                     KLATRGMLPKTTLGKAMLKKLKVYAGSEHPHTAQIKG"
     gene            complement(983..>2240)
                     /locus_tag="PKO10_03320"
     CDS             complement(983..>2240)
                     /locus_tag="PKO10_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNW66213.1"
                     /translation="IQNIDILSVILEDTKDINSIKDIIKDLSKKLNLRISIINENGEV
                     VAESHKNIEDIKNHSNRVEIIEARNIGLGKDTRVSETLNKDLIYIAKKVSFNEEIYYL
                     RMADYTNKITDNFKKLTFEIFIYISFFLIIAFISTYFISIKIQRETDSILYFLKEITN
                     KKKPIFLQSNYTFEFYKIAKLLNKVAKKISKKDEIKAKHTAKLTLANRQKDDIISAIS
                     HEFKNPIAIISGYSQTLIEDENLSQTLKIKFLNKILSNSNKMSQIVDKLRLTLKLQDN
                     NHKLILNKVSIKKIVENSISDLKIKYKNREIKVLGVDKEINADEILIGIAISNLIENA
                     LKYSQEDVIIEINENSISITDKGIGISQENLENIFKKYYRATSNNWNNSLGLGLFIVK
                     SILNVHNFKLEINSKIGNGSTFKIYY"
BASE COUNT          693 a          313 c          250 g          984 t
ORIGIN      
        1 ttcctttgaa aatatacaaa agaaaaatct tttgaaactt ccaattatct ttttcggaga
       61 aacgtttaag gtttctatcg aaattaaaaa aattaacgtt ttgagaattg agaagatttt
      121 cttgcttttt tctttccgta tttttttctt tcaacagatc tagcatctct tgtaagtaat
      181 ccatgaggtt ttaaaatagt tctaaattgc tcatcgaaag caactaaagc tcttgaaatt
      241 ccatgtctag cagcatcagc ttgagctgaa tatccaccac ctagagtttt tacaatgata
      301 tttacacttg attcttgttt agaaacatgt aatggttgca ttactctttt tttgattgaa
      361 tcatgaccac caagccattg atctaaagat tgtccattta tagttagttg tccatttcca
      421 ttttctaacc aaactttagc tattgctgtt tttcttcttc cagttgcata tacttttgcc
      481 attactatta tcctttaatt tgagcagtat gagggtgttc acttcctgca tatactttta
      541 atttttttaa catagcttta ccaagagtag tttttggaag catacctcta gttgctaatt
      601 taaacagttt ttcagggttt ttttcaatca tatctgacat tttgtgagtt tttgtacttc
      661 caaaataacc tgagtgtgta tagtaatttt taacttctaa tttagcacca gtaaatttag
      721 ctttacttgc attgattata actacgtaat caccacaatc aacgtttggt gtaaaacaag
      781 gtttattttt acctcttaaa attgtagcaa cttcagttat tattcttccg aatactttac
      841 cttcagcatc tactacaacc caagatcttt cgatttcgtt ggctttagcc atttgagtaa
      901 atttcattta ttaactccct ttcaatatta gggtcgaata atatttaagt tatcctaaat
      961 ttttgcttaa attaagtata aattaataat atattttaaa agttgatcca tttccaattt
     1021 ttgagttaat ttctaattta aaattgtgaa catttaaaat agatttaact ataaaaagtc
     1081 ctaaacctaa tgagttgttc cagttattac tagttgctct ataatatttt ttaaaaatat
     1141 tttccaaatt ttcttgagaa ataccgattc ctttatctgt aattgatatc gaattttcat
     1201 ttatctctat aattacatct tcttgtgaat attttaaggc attttctata agatttgaaa
     1261 tagcaattcc tattaaaatc tcatcagcat ttatctcttt atcgactcct aaaactttga
     1321 tttctctatt tttatacttt atttttaaat cactaataga gttttctact atttttttaa
     1381 tagaaacttt gtttaagatt agtttatgat tgttatcttg aagcttcaaa gtaagtctta
     1441 gtttatcaac tatttgtgac attttatttg agttagataa tattttattt aaaaacttta
     1501 tttttaaagt ttgcgataaa ttttcatctt ctattaaagt ttgactatat cctgaaatta
     1561 ttgctatagg atttttaaat tcatgtgaaa tagcagagat aatgtcatct ttttgtctat
     1621 ttgctaaagt taattttgca gtatgttttg cttttatttc atcttttttt gatatttttt
     1681 ttgcaacttt atttaaaagt tttgctattt tataaaattc aaaagtataa tttgattgta
     1741 aaaatattgg tttcttctta tttgttatct cttttaaaaa gtataatatt gagtcagttt
     1801 ccctttgtat tttgatactt ataaagtaag ttgaaataaa tgcaattatt aagaaaaaag
     1861 agatataaat aaaaatttca aaagttagtt ttttgaaatt atcagtaatt ttatttgtat
     1921 aatcagccat tcttaagtaa taaatctctt cattaaaaga tacttttttt gcaatatata
     1981 ttaaatcttt atttagagtt tcagatactc ttgtatcttt tccaagacct atatttcttg
     2041 cttcaataat ctcaactcta tttgagtgat tttttatatc ttcaatattt ttgtgactct
     2101 ctgctactac ttcaccattt tcatttataa ttgaaatcct taggtttagt tttttactta
     2161 aatctttaat aatatcttta atactattta tatcttttgt atcttctaaa attactgata
     2221 atatatctat attttggatt
//
LOCUS       DAOQAN010000160         2220 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_71829_length_2220_cov_2.726097, whole genome shotgun sequence.
ACCESSION   DAOQAN010000160 DAOQAN010000000
VERSION     DAOQAN010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71829_length_2220_cov_2.726097"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..205)
                     /locus_tag="PKO10_03325"
     CDS             complement(29..205)
                     /locus_tag="PKO10_03325"
                     /inference="COORDINATES: protein motif:HMM:NF022839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlcNAc-transferase family protein"
                     /protein_id="HNW66214.1"
                     /translation="MNLASYCDDMLEFTLKSAFNSAKNKENIFFGVVDQNHVNSRDTI
                     KSLEFSNQIRYCHI"
     assembly_gap    246..255
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(340..693)
                     /locus_tag="PKO10_03330"
     CDS             complement(340..693)
                     /locus_tag="PKO10_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66215.1"
                     /translation="MSKILDDLIQSQYNYNVAFYIHLKEFNQNVRDELEKRVSEVFFF
                     SKGVNWKFDSFGIEKAGERKTEILGFFIFRTAEVEKLKKLFTDEFKDYSNAIKISCSV
                     AKYEKISEEFFEIEV"
     gene            826..1959
                     /locus_tag="PKO10_03335"
     CDS             826..1959
                     /locus_tag="PKO10_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014469419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNW66216.1"
                     /translation="MQSIKTIKTIFSLFFIALFLSSCEQKNDGNQKAQNPIEVGYINP
                     KKEPINLEIELIGTVKAKELALVRRQVSGIIEKQLFKEGSFVKQGDILYKIDSATYKA
                     TLNQSLALLNSAKASLISAEAKSKRTEELLKFDGISKQEADEIKASYLQAKALVEQRA
                     AELENAKIDLNRCEIKAPISGYIGISNVTVGALVNANQSEELVNIRDTQTVFVDLSQS
                     YNEILNLKSIGDLEDDIEVSLKFDNGFEYPIKGKLEARELSVDESSQTVTLRAVFSNP
                     NNLLLSGMMTKAILKSKKSIDGFLIPQQAVLRDQKANPIVTLVTPENKTITKIVKIER
                     SVGNKWLILDALEESDKIVVEGLNKINSRSVVSLKDLNSQYKD"
     gene            1963..>2220
                     /locus_tag="PKO10_03340"
     CDS             1963..>2220
                     /locus_tag="PKO10_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNW66217.1"
                     /translation="MIAKFFIFRPIFAWVISLIIMISGVISLYILPVEQYPDIVPPQI
                     NINASYSGADAQTVENSVTQIIEQQLTGLDGMLYFSSSSSSA"
BASE COUNT          827 a          287 c          319 g          777 t
ORIGIN      
        1 tttttagcaa atgtcgattt agaaaaaatc aaatatgaca atatcttatt tgatttgaaa
       61 actcaagaga ttttattgta tctctactgt ttacatgatt ttgatcaact actccaaaaa
      121 agatattttc tttatttttt gcactattaa atgcactttt tagtgtaaat tccaacatat
      181 catcgcaata tgatgctaga ttaataaata ttttattatt aaaattatat ttcccaattt
      241 attttnnnnn nnnnncttct atttttttta atcggtttta aaagtagtcc actttcaact
      301 acttctaaat caatttcaac attttcaagt tgagttatat taaacttcaa tctcaaaaaa
      361 ctcttcactt attttttcat attttgcaac agaacaagat atttttatag catttgaata
      421 atctttaaac tcatctgtaa aaagtttttt tagcttttca acttctgctg ttctaaatat
      481 aaaaaatcct aatatttcag tttttctctc acctgctttt tctattccaa agctatcaaa
      541 tttccagttt acaccttttg aaaagaagaa aacttcacta actctttttt caagttcatc
      601 tcttacattt tggttgaact cttttaaatg tatataaaat gcaacattat agttgtattg
      661 tgattgtatt aaatcatcta agattttact catttttcgc cttttttatt tgttttttat
      721 atgttatcaa tattttgcta caatttttct tcaatatata gaaatattaa aattttgtta
      781 ataaagttat aataacattc ataaattctt attcaaggga aagcattgca gagtattaaa
      841 actatcaaaa ctatattttc actatttttt atagcacttt ttttaagctc atgtgagcaa
      901 aaaaatgatg gaaatcaaaa agcacaaaat ccaatagaag tagggtatat aaatcctaaa
      961 aaagagccaa taaatcttga aattgaactt ataggaacag taaaagcaaa agagttagca
     1021 cttgttcgac gtcaggtttc tggaatcatt gaaaaacaac ttttcaaaga gggaagtttt
     1081 gtaaaacaag gtgatatttt gtacaaaatt gatagtgcaa catataaagc tactttaaat
     1141 caatcacttg ctttattaaa tagtgcaaag gctagtttga taagtgctga agcaaagagt
     1201 aaaagaacag aagagctttt aaaatttgat ggaatttcaa aacaagaagc agatgagata
     1261 aaagcttcat atttgcaagc caaagctttg gttgaacaaa gagctgcaga gcttgaaaat
     1321 gcaaaaatag atttgaatag atgtgagata aaagcaccaa ttagcggata tattggtatt
     1381 tcaaatgtaa ctgttggggc tttggtaaat gcaaatcaaa gtgaagagct tgtaaatata
     1441 agagatacac aaacagtttt tgtagattta tcacaatcat acaatgagat tttaaatcta
     1501 aaatcaattg gtgatttaga agatgatatt gaagttagtt tgaagtttga caacgggttt
     1561 gagtatccaa taaaaggaaa actagaagca agagagttaa gtgtagatga gagtagccaa
     1621 acagtaactt taagagctgt atttagtaat ccaaataatc ttttattatc tggaatgatg
     1681 acaaaagcaa ttttaaaaag caaaaaaagt attgatggat ttttaattcc acaacaagca
     1741 gttttaagag atcaaaaagc aaacccaatc gttactcttg tgacacctga aaataaaact
     1801 atcacaaaaa tagtaaaaat agagagatca gtaggtaata aatggcttat tcttgatgct
     1861 ttagaggaga gtgataaaat tgtagttgaa ggattaaata agataaatag tagaagtgta
     1921 gtctctttaa aagatttaaa ctctcaatac aaagattaag aaatgattgc aaaatttttt
     1981 atttttcgtc caatctttgc ttgggttatt tcacttatta ttatgataag tggggttatt
     2041 agcctatata ttttaccagt tgaacagtat ccagatattg ttcctcctca aataaatata
     2101 aatgcttcat atagtggagc agatgcccaa acagtagaaa atagtgtaac tcaaataatt
     2161 gagcaacaac taactggtct tgatggaatg ttatatttct cttcaagttc aagtagtgca
//
LOCUS       DAOQAN010000161         2216 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_72021_length_2216_cov_2.338732, whole genome shotgun sequence.
ACCESSION   DAOQAN010000161 DAOQAN010000000
VERSION     DAOQAN010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72021_length_2216_cov_2.338732"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>516)
                     /locus_tag="PKO10_03345"
     CDS             complement(<1..>516)
                     /locus_tag="PKO10_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit G"
                     /protein_id="HNW66218.1"
                     /translation="VDLGSNVMLDQERCVLCLRCVRFCKDITKTAELGVIDRTDHSVI
                     GTFPGRPLDNPYAMNVVDLCPVGALTSKDFRFKQRVWFLQSFEAICNGCSKGCNINVD
                     HRKEKYKDDMIYRFRPRTNRAVNGWFMCDYGRLSYENDATNRFTTALINKNETNISNA
                     IINIFKELSSHD"
     assembly_gap    517..526
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<527..907)
                     /locus_tag="PKO10_03350"
     CDS             complement(<527..907)
                     /locus_tag="PKO10_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNW66219.1"
                     /translation="MADQVSITINGVQFQATKGSLLIDKLLDEKIHIPHFCYHQALGK
                     DGNCRMCMVEIEGQKRPQIACDTPIKDGMIVRTKGEKIESVRRDILELELINHPIDCP
                     TCDQAGECKLQDYYMESGFYASRVN"
     gene            complement(929..2215)
                     /locus_tag="PKO10_03355"
     CDS             complement(929..2215)
                     /locus_tag="PKO10_03355"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013136450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HNW66220.1"
                     /translation="MAKNTMTFTDNRTGKSYEYNIIDGTRGPSVVDISTFYKDSGMFT
                     FDPGYTSTAACESKITFIDGENSELKYRGIDIADLAGKHSFLDVSYLLMNGRLPTKDE
                     SKNLDLEIRHRSFVDEGIIRLFDALPDRAHPMATMAASTMALSAFYKDHLHLEDEEEF
                     RTMQNRIMAKMPTIAAMAYRNSIGTPMIYPDIDRYFTENFLYMLRAYPGGKMKHLADG
                     SNQEISQIEVDALDAILTLHADHEQNASTTTVRNVGSTEAHPYVAIASGISALWGAAH
                     GGANEKVMDQLRMIGDVKNVPSFIAKAKDKNDPFRLMGFGHRVYKNRDPRAETLKGLQ
                     DQLREKLNLDSKLLDIAKAVEEAALSDDYFKERGLYPNIDFYSGVILTALKIPVEMFT
                     PIFVIGRTPGWIAQWAEFKRDPKHKIARPRQLYTGK"
BASE COUNT          673 a          424 c          280 g          829 t
ORIGIN      
        1 atcatgactt gataactctt tgaagatatt aataatagca tttgaaatat ttgtctcatt
       61 tttattaatt aatgcagttg taaatctatt tgttgcatca ttttcataag ataatcttcc
      121 ataatcacac ataaaccaac cgtttactgc tctatttgtt cttggtctaa atctataaat
      181 catatcatct ttatatttct cttttctatg atctacattt atattacacc cttttgaaca
      241 accattacaa atagcttcaa aactttgtaa aaaccaaact ctttgtttaa atctaaaatc
      301 tttgcttgtt aatgctccaa caggacataa atcaacaaca ttcattgcgt atggattatc
      361 taaaggacgt ccaggaaatg tcccaatcac agagtgatct gttctatcta taactcctag
      421 ttctgccgtt tttgtaatat ctttacaaaa tcttacacat ctaagacata aaacacatct
      481 ctcttgatcc aacattacat ttgaacctaa atcaacnnnn nnnnnnattt actcttgaag
      541 cataaaaacc agattccata tagtaatctt gtagtttaca ctcaccagct tgatcacatg
      601 taggacagtc tattgggtga tttataagtt caagttctaa aatatctctt ctaacactct
      661 ctattttttc accttttgtt cttacaatca ttccatcttt tataggtgta tcacatgcaa
      721 tttgaggtct tttttgcccc tctatttcaa ccatgcacat tctacaattt ccatcttttc
      781 ccaacgcttg atgataacaa aagtgaggga tatggatttt ttcatccaat agtttatcaa
      841 ttaacaagct acctttggta gcttgaaatt gaactccatt aattgttata ctaacttgat
      901 cagccataaa ttcatatccc cttatttttt atttacctgt atataattgt ctaggtctag
      961 caattttatg ttttggatct cttttaaact ctgcccattg agcaatccat cctggagttc
     1021 ttcctataac gaaaatcggt gtaaacatct ctactggaat tttaagcgct gttaaaatta
     1081 ctcctgaata gaaatcaata tttggataca atcctctctc tttaaaataa tcatcactta
     1141 aagctgcttc ttcaactgct tttgcaatat ctagaagttt agaatctaag tttaatttct
     1201 ctcttaattg atcttgtaaa ccttttaatg tttcagctct tgggtctcta tttttataaa
     1261 ctctatgacc aaaccccata agtctaaatg gatcattttt gtctttagct tttgctataa
     1321 agctaggaac attttttaca tcaccaatca ttcttaactg atccataact ttttcatttg
     1381 ctccaccatg agctgcaccc caaagtgctg aaataccaga agctattgca acatatgggt
     1441 gagcttcagt tgaaccaaca tttctaactg ttgttgtaga agcattttgt tcatggtctg
     1501 catgaagagt taaaattgca tcaagagcat caacttcaat ttgactaatt tcttgatttg
     1561 aaccatcagc taaatgtttc atttttccac ctggatatgc tcttagcata tacaagaagt
     1621 tttctgtgaa atatctgtca atatctggat aaatcatagg tgttccaatt gagtttctat
     1681 aagccatagc tgcaattgtt ggcatttttg ccataattct attttgcata gttctaaact
     1741 cttcttcatc ttctaaatgt aagtggtctt tataaaatgc agatagtgcc attgttgaag
     1801 ctgccattgt tgccataggg tgagctctat caggtaaggc atcaaatagt cttataattc
     1861 cctcatcaac aaaagatcta tgtcttattt ctaaatcaag attttttgac tcatcttttg
     1921 ttggtaatct tccattcatt agtagatatg atacatctaa aaatgagtgt ttaccagcta
     1981 aatcagctat atcaattcct ctatatttta gctcagagtt ttcaccatca ataaaagtaa
     2041 tttttgactc acaagctgct gttgaagtat atccagggtc aaatgtaaac attcctgaat
     2101 ctttataaaa agtagaaata tctacaacac ttggtcctct tgtaccatca ataatattgt
     2161 actcataaga tttaccagtt ctgttatctg taaaagtcat tgtatttttt gcgatt
//
LOCUS       DAOQAN010000162         2213 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_72148_length_2213_cov_3.299815, whole genome shotgun sequence.
ACCESSION   DAOQAN010000162 DAOQAN010000000
VERSION     DAOQAN010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72148_length_2213_cov_3.299815"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2213
                     /locus_tag="PKO10_03360"
     CDS             <1..>2213
                     /locus_tag="PKO10_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014467927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HNW66221.1"
                     /translation="ASYISLEPALTGAQANGLLARYSKKIGPDPASINAAMIGGIAAN
                     NASGMCCGISQNSYKTVKSMKLIFSDGTKLDTADENSKNEFRKTHKDFLENLNKFSRE
                     TKSNEELRAKIEKKFKIKNTCGYSINALIDFEDEFEILQHLIIGSEGTLAFIEEITYY
                     TVEDLKDKASALIYFKDMNEACHAVTKLKLARDSKEIIVDAVELMDRAALRSIENDPV
                     MPEFIKDFDEDVTALLIETRALSDEKLNIQIEQIEDLLKEFEVKRKIYFTKDEQEYNL
                     YWKIRKGLFPAVGAVRVTGTTVIIEDVAYPIEVLAEATLELQGLFQKYGYSEALIFGH
                     ALEGNFHFVFTQDFSDVKEVKRYDDLMNEVVNSVAVKYQGSLKAEHGTGRNMAAFIEV
                     EWGKEAYNIMKKIKSLFDPKGLLNPGVIINDDKEAHLKNLKTLPATNEIVDKCIECGF
                     CEPTCPSNELTLTPRQRIVINREISRLESIGNYKEAKEYKELYQYDGIETCATCSLCS
                     TACPVKIDTGSLTKHLRAEQLTSKEKSIANFVANNFGITLKGIRFGLHSANFIHRTLG
                     TSNMEAFTKTMRDLSKNKLAKWSVYMPKGTNINLNFEQQVKDKKVVYFPSCISRTMGL
                     NDMSKEEKELFEVTIEVLQKAGYQIIFPQNLSNLCCGMPFSSKGFNDAAHTKSRQLEE
                     ALLNASEFGEYPILCDTSPCTKKIMESFSHKLSIYEPIEFALEFLTKDLEFTPID"
BASE COUNT          893 a          269 c          377 g          674 t
ORIGIN      
        1 atgctagtta tatctcacta gagccagcat taacaggtgc acaagcaaat ggattacttg
       61 cacgttattc taaaaaaatt ggtccagatc cagcaagtat aaatgcagct atgattggtg
      121 gaattgcagc aaataatgct tctggaatgt gctgtggaat ttcacaaaat tcatacaaaa
      181 ctgtaaaatc aatgaaacta attttttcag atggaactaa acttgataca gcagatgaaa
      241 atagtaaaaa tgagtttaga aaaactcaca aagatttttt agaaaattta aataagtttt
      301 cacgtgaaac aaaatcaaat gaagagttaa gagcaaaaat agagaaaaaa tttaaaataa
      361 aaaatacatg tggttactca ataaatgcct taattgattt tgaagatgag tttgaaatac
      421 ttcaacactt aattattgga agtgaaggaa ctttagcatt tatcgaagag ataacttatt
      481 atacagttga agatttaaaa gacaaagcaa gtgccttgat ttattttaaa gatatgaatg
      541 aagcctgtca tgcagttaca aaattaaaat tagcacgtga ttctaaagaa attattgttg
      601 atgcagttga acttatggat agagcagctt taagaagtat tgaaaatgac cctgttatgc
      661 cagaatttat caaagatttt gatgaagatg taactgcact tttaattgaa acacgagctt
      721 taagtgatga aaaattaaat atccaaatag agcaaattga agatttacta aaagagtttg
      781 aagtaaaaag aaagatttat ttcacaaaag atgaacaaga atataatctt tattggaaaa
      841 ttagaaaagg attatttcca gctgttggag cagtaagagt tacaggaaca acagttatta
      901 tagaagatgt tgcttatcct attgaagttt tagctgaagc tacacttgaa cttcaaggct
      961 tattccaaaa atatggttat agtgaagctt taatctttgg acatgcactt gaaggaaact
     1021 tccactttgt atttacacaa gatttctctg atgtaaaaga ggtaaaaaga tatgatgatt
     1081 taatgaatga agttgttaat tctgttgcag ttaaatacca aggaagttta aaagctgaac
     1141 atggaactgg acgaaatatg gctgcattta ttgaagtaga gtggggtaaa gaagcttata
     1201 atataatgaa aaaaatcaaa tcattgtttg atccaaaagg tttattaaac cctggagtta
     1261 taataaatga tgataaagaa gctcacttaa aaaatctaaa aacacttcca gcaacaaatg
     1321 aaatagttga taaatgtatt gagtgtggtt tttgtgaacc aacatgtcct tcaaatgaat
     1381 tgactttaac tccaagacaa agaattgtaa taaatagaga aatctcaaga ctagaatcta
     1441 ttggaaatta taaagaagca aaagagtaca aagagttata tcaatatgat ggaatagaaa
     1501 cttgtgcaac atgtagttta tgttcaactg cctgtcctgt aaaaatagat actgggagtt
     1561 taacaaaaca tttaagagca gagcaattaa cttctaaaga aaaatctatt gcaaattttg
     1621 tagcaaacaa ctttggtatt acattaaaag gtatcagatt tggtcttcat agtgctaatt
     1681 ttatacacag aactttagga acttctaata tggaagcttt tactaaaact atgagagatt
     1741 taagtaaaaa taaattagca aaatggtcag tttatatgcc aaaaggaaca aatataaact
     1801 taaattttga acaacaagta aaagataaaa aagttgtata tttcccttca tgtattagta
     1861 gaacaatggg attaaatgat atgtcaaaag aggaaaaaga actctttgaa gtaacaattg
     1921 aagttttaca aaaagctggt taccaaatca tcttccctca gaatttatca aatctttgtt
     1981 gtggaatgcc attttcttca aaaggattta atgatgcagc gcatacaaaa tcaaggcaac
     2041 ttgaagaggc acttttaaat gcaagtgaat ttggagaata tccaatttta tgtgatacaa
     2101 gtccatgtac taaaaaaatc atggaaagtt tttctcataa attatcaatt tatgaaccaa
     2161 tagagtttgc attagagttt ttaacaaaag atttagaatt tactccaatt gat
//
LOCUS       DAOQAN010000163         2197 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_72970_length_2197_cov_2.969188, whole genome shotgun sequence.
ACCESSION   DAOQAN010000163 DAOQAN010000000
VERSION     DAOQAN010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72970_length_2197_cov_2.969188"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="PKO10_03365"
     CDS             complement(<1..221)
                     /locus_tag="PKO10_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66222.1"
                     /translation="MKNLIFTLIFIFSSNFLFAKELEATFSFFASSGVTNIAKDKQNL
                     YLATNDGFVDIFNLEKKEIISSIKIKNIK"
     gene            complement(230..709)
                     /locus_tag="PKO10_03370"
     CDS             complement(230..709)
                     /locus_tag="PKO10_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013327048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNW66223.1"
                     /translation="MQRRELFGSLAKPFKNRSLQEKAIRPPYFKDINLFFTKCLECLE
                     KPCLISCEENIIEILEDGTPKINFSNSGCTYCDLCAISCQNGVLNIEDKKQIEAKIEI
                     DVLTCLSWQNTMCFSCKDPCLENAIDFLGMFRPTINQNCSSCGFCIKVCPTNAIKIA"
     gene            complement(712..1314)
                     /locus_tag="PKO10_03375"
     CDS             complement(712..1314)
                     /locus_tag="PKO10_03375"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HNW66224.1"
                     /translation="MKLKKLALSLVAIATLFTFAYAATKSIKDESLGIRQDNLFSEDK
                     VVSDETKYSTEPAGTSTKIKRAFENAPPMIPHDTEGMLPITIDNNQCTSCHDPMVAES
                     MGATPIPKSHFTSFREDVNIDKKGELQRDGKVVANGSDLKTIIKPLDHLSNARFNCSA
                     CHAPQSDSKIVPKNNFQTEFRSSDLNDKSNLIDTINEGVK"
     gene            complement(1311..2114)
                     /gene="napH"
                     /locus_tag="PKO10_03380"
     CDS             complement(1311..2114)
                     /gene="napH"
                     /locus_tag="PKO10_03380"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014473205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HNW66225.1"
                     /translation="MKKYRFLIARRFTQLSIIALYILANIYGINILMGNLSSSLVLQT
                     VTLSDPFAVLQMIFAGAIISFDIALGALIISIFYFILGGRAFCSWVCPVNIITDSANY
                     LRRVLKFDEVQKKQPATRNLRYWLILISFIISYFMGVAAFELISPVSMVHRGLIFGLG
                     FGLATMIVIFLFDLFVLKNGWCGHICPLGGFYSLIGRFSLLRVHHNSEKCTACMKCKV
                     VCPEIQVLHMIDKSSEPVLQECTLCARCIEVCEDDALNFELRNYMKEKK"
BASE COUNT          759 a          366 c          274 g          798 t
ORIGIN      
        1 tcttttatat ttttaatttt tattgaagat attatctctt ttttttcaag gttaaaaata
       61 tcaacaaagc catcatttgt tgctaaatat aagttctgtt tatcttttgc aatgtttgta
      121 actccacttg atgcaaaaaa agagaaagtt gcttctaact cttttgcgaa taaaaagttt
      181 gagctaaaaa taaaaataag tgtaaaaatt aagtttttca aaacaaatcc tatgctattt
      241 tgattgcatt tgttgggcat acttttatac aaaagccaca agaactgcaa ttttgattta
      301 tagttggtct aaacattcct aaaaaatcta ttgcattttc caagcaagga tctttacatg
      361 aaaaacacat agtattctgc caagatagac atgttagaac atcaatctct attttagctt
      421 ctatttgctt tttatcttca atatttaaaa caccattttg acaagaaatt gcacataagt
      481 cacaataagt acaaccagaa tttgaaaaat ttatttttgg agtaccatct tctaaaatct
      541 ctataatatt ttcttcacaa gaaattaagc aaggtttttc tagacattcc aaacattttg
      601 taaaaaaaag atttatatct ttaaagtagg gtggtcttat agctttttct tgcaaacttc
      661 tatttttaaa aggttttgca agagagccaa aaagctctct tctttgcata cttattttac
      721 accctcattt atagtatcta taagattcga tttgtcattt aaatcactac ttctaaactc
      781 tgtttggaag ttattttttg gaacaatttt actatctgat tgtggtgcat ggcaagcaga
      841 acagttaaat ctagcatttg ataagtgatc aagaggtttt ataatagttt ttaaatcact
      901 accatttgct acaacttttc catctctttg aagttcacct tttttatcta tatttacatc
      961 ttctctaaaa cttgtaaagt gtgattttgg aataggagtt gcacccatag attctgcaac
     1021 cattgggtca tgacaagaag tacactggtt attatctatt gtaataggta acattccttc
     1081 tgtatcatgt ggaatcatag gtggagcatt ttcaaatgct ctttttattt ttgtactagt
     1141 tcctgcaggt tctgtagaat attttgtctc atcacttaca actttatctt ctgaaaatag
     1201 attatcttgt ctaattccaa gagattcatc ttttatactt ttagttgccg cataagcaaa
     1261 agtaaaaagt gttgcaatgg caactagact taaagctaat ttttttaatt tcattttttc
     1321 tccttcatat aatttcttaa ttcgaaattt agtgcatcat cttcacaaac ttcaatacat
     1381 ctagcgcata aagtacactc ttgtaaaaca ggttcgctac ttttatctat catatgtaaa
     1441 acttgaattt cagggcaaac aactttgcat ttcatacaag cagtacattt ttcactatta
     1501 tgatgaactc ttaatagact aaatcttcca attagtgaat aaaatccacc aagaggacaa
     1561 atatgtccac accaaccatt ttttaaaaca aacaggtcaa acaaaaatat tacaatcatt
     1621 gttgcaagtc caaatccaag tccaaagatt aaacctctgt gaaccattga tacaggagaa
     1681 atcaactcaa aagctgcaac gcccataaaa taagagatta taaaactaat taaaattagc
     1741 caatatctta aatttctagt tgctggttgt tttttttgaa cttcatcaaa tttcaaaact
     1801 cttcttaggt aatttgcact atctgtaatt atatttacag ggcaaaccca agagcaaaaa
     1861 gctcttcccc caagaataaa gtagaaaatt gaaataatta aagcacctaa agctatatca
     1921 aaagagatta tagctcctgc aaaaatcatt tgtaaaactg caaaagggtc gcttagggta
     1981 acagtttgta aaaccaaaga gctacttaaa ttccccatta atatatttat tccataaata
     2041 tttgccaaaa tatataaagc tataatagag agttgtgtaa atcttctagc aattaaaaat
     2101 ctatattttt tcattctaat aaatccttca tatcgctatt tagtgactct actgcacctt
     2161 ttttatttat tttagtttta cctaactcaa catttga
//
LOCUS       DAOQAN010000164         2194 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_73133_length_2194_cov_2.722300, whole genome shotgun sequence.
ACCESSION   DAOQAN010000164 DAOQAN010000000
VERSION     DAOQAN010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73133_length_2194_cov_2.722300"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..1730)
                     /locus_tag="PKO10_03385"
     CDS             complement(78..1730)
                     /locus_tag="PKO10_03385"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014469409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HNW66226.1"
                     /translation="MNASDLFIKALENEGVEYIFGVPGEENLDFLEAMRKSNIKLILT
                     RHEQGAGFMAATYGRLTGKVGVCLATLGPGATNFATCAAYAQLGGMPMMMITGQKPIK
                     KSKQGRFQIIDIVRMMRPMTKFAKQIVNVHNIPSVVRDAFKIATTERPGAVHIELPED
                     IAHEPVDADTTIYPVYNVKYPAAVDEVIAEAVAMIEKAKRPLLLIGAGANRTRIGNTL
                     TDLVNKTGMAFFSTQMGKGVIDENHPMCLSAAALSKDDFVHCAIDRADLIINVGHDVI
                     EKPPFFMSNKEGATKVMHVNFFPSEVDDTYFPQMDVVGDIASNIAKITEKIKVQAHWD
                     FDYYTRMAEEIRTRLSKYFGDNRFPILPQRAVRAIRKTLAAEDIVTLDNGVYKLWFAR
                     NYRCAKPNTLLLDNALATMGAGLPSGMMAKMINPNKKVVSVCGDGGFMMNSQEMETAV
                     RLGLDLTVIILNDNAYGMIKWKQTGMGFETFGLDLGNPDFVKYAESYGATGHRPASVE
                     EFEKTLDACVNGKGVHLIDLAVDYSLNHSILNDLLANKQCIL"
     gene            1940..>2194
                     /locus_tag="PKO10_03390"
     CDS             1940..>2194
                     /locus_tag="PKO10_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HNW66227.1"
                     /translation="MKKTVIFDLDGTLLDSIEDIASSMNKVLESLQLPTHKIEDYKYF
                     VGGGVDILVENALNNQSKEIKDEVIKRFKIEYDGKLHSKTL"
BASE COUNT          726 a          392 c          290 g          786 t
ORIGIN      
        1 ggaacagaac ctactacttt gccatcaaat ggtgatgtta cttctactgt tgtactcata
       61 tttttccttt cagttattta tagaatacat tgtttgtttg ctaataaatc atttaaaatt
      121 gaatgattta aagagtaatc aactgctaaa tcaattaaat gaacaccttt accatttaca
      181 caagcatcta aagttttttc aaactcttca acagaagctg gtctatgtcc agttgcacca
      241 taagattcag catatttaac aaaatcaggg tttcctaaat ctaaaccaaa tgtttcaaat
      301 cccattcctg tttgtttcca tttaatcatt ccatatgcat tatcatttag aataattaca
      361 gttaaatcaa gtcctaatct aacagctgtc tccatctctt gagagttcat cataaaccca
      421 ccatctccac aaactgaaac aactttttta tttggattga tcatttttgc catcattcca
      481 gaaggaagtc cagctcccat agttgcaagt gcattatcta gtaaaagtgt atttggtttt
      541 gcacatctat agtttcttgc aaaccataat ttataaacac cattatcaag cgttacaata
      601 tcttcagcag ctaatgtttt tctaatagct cttacagctc tttgaggtaa aattgggaat
      661 cgattatcac caaaatattt tgataatcta gttctaatct cttctgccat tcttgtatag
      721 taatcaaaat cccagtgagc ttgaactttt attttctcag ttatttttgc aatattactt
      781 gcgatatctc caacaacatc catttgtggg aagtaagtat catctacttc agatgggaag
      841 aagttaacat gcataacttt tgttgcaccc tctttatttg acataaaaaa tggtggtttt
      901 tcaataacat catgtccaac attgataatt aaatctgctc tatcaatcgc acagtgaaca
      961 aagtcatctt ttgataatgc agcagcactt aaacacattg gatgattttc atcaataaca
     1021 cctttaccca tttgagtaga gaagaatgcc ataccagttt tatttactaa atctgttaaa
     1081 gtatttccta ttcttgttct atttgctcca gctccaatta aaagtagtgg tcttttagct
     1141 ttttcaatca tagcaactgc ttctgcaata acctcatcaa ctgctgctgg atattttaca
     1201 ttgtaaactg ggtagatcgt tgtatcagca tctactggct catgtgcaat atcttcaggt
     1261 agttcaatat gaacagcacc tggtctttca gttgttgcaa ttttaaatgc atctcttaca
     1321 acagaaggga tattgtgaac atttacaatt tgttttgcaa atttagtcat cggtctcatc
     1381 attcttacga tgtcaataat ttggaatctt ccttgttttg attttttaat tggtttttga
     1441 ccagttatca tcatcatagg cattccacct aattgtgcat aagcagcaca agttgcaaag
     1501 tttgtagcac caggcccaag agttgctaaa caaactccaa cttttcctgt taatcttcca
     1561 taagttgcag ccataaatcc tgcaccttgc tcatgtcttg ttaagataag tttgatattt
     1621 gattttctca tagcttccaa gaaatctaag ttttcttcac ctggaacacc aaaaatgtac
     1681 tcaacacctt cgttttctaa tgctttaata aataaatctg atgcattcat ctttctttta
     1741 ctccgtttta attaatagtt gtaataattc acatagctaa ttttaaatat aaaaaatatc
     1801 ttattttttg atacttaata aacaattgac attataacca tattaaaact attctttata
     1861 taatattaac tatatttaca catcttataa ctttatatta acaaaaagtt acactttaga
     1921 taaaaggaga atttatagaa tgaaaaaaac agttatattt gacctagatg gcacactttt
     1981 ggactcaatt gaagatatag catctagtat gaataaagtt cttgaaagct tacagcttcc
     2041 aacacataaa attgaagatt ataaatattt tgttggagga ggagttgata ttttagtaga
     2101 aaatgcttta aataatcaat caaaagagat aaaagatgaa gtaataaaaa gatttaaaat
     2161 tgagtatgat ggaaaactac actcaaaaac tcta
//
LOCUS       DAOQAN010000165         2188 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_73397_length_2188_cov_3.455696, whole genome shotgun sequence.
ACCESSION   DAOQAN010000165 DAOQAN010000000
VERSION     DAOQAN010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73397_length_2188_cov_3.455696"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            163..477
                     /locus_tag="PKO10_03395"
     CDS             163..477
                     /locus_tag="PKO10_03395"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HNW66228.1"
                     /translation="MKKIVLLVLAFAGFAFAADAAVANETLKAYSVVAAGIGLGLAAL
                     GGAIGMGNTAAATIAGTARNPGLGGKLMTTMFIALAMIEAQVIYALVIAMIALYANPF
                     LG"
     gene            complement(541..1509)
                     /locus_tag="PKO10_03400"
     CDS             complement(541..1509)
                     /locus_tag="PKO10_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNW66229.1"
                     /translation="MKKIVLVFTLLLNLFAKDENTIVIAGPIASVSHPILYMIEQNAL
                     KDIGKKIEFKLWNNPDELRALILKKEVDFIALPTNVAANLYNKGIDLKLLNVSTWGIL
                     GLVSRDKNLKNIEDFKNKEIIVPFRADMPDIIFQALIKKANLNIKKDFKLTYVATPID
                     AMQMLILRRADHALLAEPAISIALRKTGSFPVKLIAPDLYRSVDLQKDWGRLFEVEPK
                     IPQAGLAIIGETKGKEQLITKILEEYEKAINWYKSNQKDASELVVKTLPMLEVNGLAD
                     SIDYIKFENINAQNSKKDLEFFFSVLLENDSKIIGGKLPDDNFYYK"
     gene            complement(1531..>2188)
                     /locus_tag="PKO10_03405"
     CDS             complement(1531..>2188)
                     /locus_tag="PKO10_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HNW66230.1"
                     /translation="LFLVLILLALRRVSMESINELLNQENISEIFLAKSFRYNLAIFC
                     LLLYGFVELFFPNNSTLAYICFACGSATFALLNDFVLKDNNILFKPFVLYIISTISIT
                     AIGFFFLGFNYLFELNITNHFRHFLTTGSFGMVFYVIMIIVSTIHTGRKIFTNWALTL
                     GLILIIIATFMRAFIPFYIEYSMALYILSSIIWAIPFIIYMKIFFPFLLQVRADGIKG
                     "
BASE COUNT          782 a          331 c          277 g          798 t
ORIGIN      
        1 ataataaata taattaatat aaattttttc ataaatcact tcccaaagtt taaattgctt
       61 tatcttactt ttagtttact taaaatataa tctcagccat caaagtactt tgtagggata
      121 cgcaagccga acagactttg aaaatattta taaggattta agatgaaaaa aatcgttctt
      181 ttagtactag cattcgctgg ttttgcattt gctgcagatg ctgcagttgc aaatgagact
      241 ttaaaagcat actctgtagt tgctgctgga attggacttg gattagctgc tcttggtgga
      301 gctattggta tgggtaatac tgctgctgca actattgctg gaactgctag aaacccaggt
      361 cttggtggaa aattaatgac tacaatgttt attgcattag ctatgattga agcacaagtt
      421 atttatgcac ttgttattgc tatgatcgca ctttatgcaa atccattttt agggtaagct
      481 tttaaatcta accagattta ataaaaacaa aaaggcttga gattttatct caagcctttt
      541 ttatttgtag taaaaattat catctggtaa cttcccacca attattttac tatcattttc
      601 aagtaataca ctaaagaaaa actctaaatc ttttttacta ttttgagcat ttatattttc
      661 aaatttaata taatcaattg aatcagctaa accattaacc tccaacatag gtagtgtttt
      721 aactacaagt tcactagcat ctttttgatt tgatttatac cagtttatag ctttttcata
      781 ctcttctaag attttagtaa tcaactgctc ttttcctttt gtttcaccaa tgattgctaa
      841 tcctgcttgt ggaatttttg gttcaacttc aaataatctt ccccaatctt tttgtaaatc
      901 aacacttcta tataaatcag gggctattaa cttaacagga aaagagcctg tttttcgtag
      961 cgcaatagaa attgcaggtt ctgctaaaag tgcatgatct gctcttctta aaatcaacat
     1021 ctgcatagca tcaataggag ttgcaacata tgttaattta aaatcttttt ttatattaag
     1081 atttgctttt tttataagtg cttgaaaaat aatatcaggc atatcagctc taaatggaac
     1141 aataatttcc ttatttttaa aatcttctat gttttttaaa tttttatcac gactaacaag
     1201 tcccaaaatt ccccaagttg aaacatttaa taacttcaaa tctatccctt tgttgtataa
     1261 atttgcagct acatttgtag gaagagctat aaaatcaacc tcttttttaa gtattaaggc
     1321 tcttagttca tcaggattat tccaaagttt aaactctatt ttttttccaa tatcttttaa
     1381 agcattttgt tctatcatat acagtattgg atgagataca cttgctattg gtcctgctat
     1441 tactattgta ttctcatctt ttgcaaataa gtttaataaa agtgtaaata ctaaaactat
     1501 ttttttcata aataaatcct ttttattttt ttaacctttt atcccatcag ctcgaacttg
     1561 tagtagaaat ggaaaaaata ttttcatata tattataaaa ggtattgccc aaataattga
     1621 agatagaata tataaagcca tactatattc aatgtaaaat ggaataaaag ctctcataaa
     1681 tgttgcaata attattaaaa ttaaacccaa agttaaagcc caatttgtga aaatttttct
     1741 tcctgtatga atcgttgata ctataatcat aattacataa aaaaccattc caaaacttcc
     1801 tgttgttaaa aaatgtctaa agtggtttgt gatatttaac tcaaaaaggt agttaaaacc
     1861 taaaaagaaa aatccaatcg cagttataga aattgtagag attatgtata aaacaaaagg
     1921 tttaaataaa atattattat cttttaaaac aaaatcgttt aaaagagcaa aagtagctga
     1981 accacaagca aaacaaatat aagctaaagt tgaattattt ggaaaaaaga gttcaacaaa
     2041 accatataaa agtagacaaa atatagcaag gttgtatcta aaagattttg ctaaaaatat
     2101 ttcacttata ttttcttgat ttaataactc atttattgac tccatactaa ctcttcttaa
     2161 agccaataaa attaaaacca aaaataaa
//
LOCUS       DAOQAN010000166         2188 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_73413_length_2188_cov_2.704641, whole genome shotgun sequence.
ACCESSION   DAOQAN010000166 DAOQAN010000000
VERSION     DAOQAN010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73413_length_2188_cov_2.704641"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="PKO10_03410"
     CDS             complement(<1..202)
                     /locus_tag="PKO10_03410"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014667543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HNW66231.1"
                     /translation="MKVAVLQFPGTNCEFDAKYAFTKLGCDVEVIWHKDTKLPENTDL
                     VVVPGGFSYGDYLEVEQLLDLQI"
     gene            complement(205..450)
                     /gene="purS"
                     /locus_tag="PKO10_03415"
     CDS             complement(205..450)
                     /gene="purS"
                     /locus_tag="PKO10_03415"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HNW66232.1"
                     /translation="MKAIVNVGLKKGVLDDQGKAINHALGTLGFKDLISDVRVGKQII
                     IELNSNDKEKAKEEVTKMCEKLLANTVIEDYNIDIVG"
     gene            complement(492..1445)
                     /locus_tag="PKO10_03420"
     CDS             complement(492..1445)
                     /locus_tag="PKO10_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014469388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HNW66233.1"
                     /translation="MNIEKIKSLNLWPENKKITTQKGFDELENIGYNLFYIGKNADLY
                     SCPGDEAKVLLVRSDRTSVFDIPLNFEIKGKGIIQTEISNFGAKFAANSGIRTAILDE
                     KISSDISVAPRCQLMKLCKALEANIDGEVVQFELIFRNYLTGSLFQALQDGKDPYGLD
                     LPSNLKEWHKFETPLFTPTTKGLKDEPLNSKKVRDTFPEIISSLEKLFKDFTTFAEDR
                     GIVVVDTKFEIFVDEDGKWVLGDEVLTPESSRFIALEDFKNSNFISMDKQILRDFGKK
                     ENWKEQSKSLKSGEKLEVVVPKEIEDKILNGYKTILERLSK"
     gene            complement(1459..>2188)
                     /locus_tag="PKO10_03425"
     CDS             complement(1459..>2188)
                     /locus_tag="PKO10_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013134220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNW66234.1"
                     /translation="GENLLYLRISSFDTKVTNDLEKAIKENKDVKGIVLDLRNNPGGL
                     LNQAIGVVDLFVKNGVIVSQKGRDASDEEKFEASKFGTKTDLPLVVLVNEGSASASEI
                     VSGALQDHKRAILVGEKTFGKGSVQAVLPIDNEKTENIKLTIAKYYLPSGRTIQAEGV
                     TPDIIASAGKVVQSEENGLKIKEADLKKHLEGELNKVDDKLKAEEKAVKDETKKIISK
                     DDLQNDNQLNTSLAVLKSLIIMNK"
BASE COUNT          679 a          401 c          234 g          874 t
ORIGIN      
        1 atatttgcaa atctagcaat tgctccactt ctaagtagtc accataagag aacccacctg
       61 gaacaactac taaatcagta ttttctggaa gttttgtatc tttgtgccaa ataacttcta
      121 catcgcaacc aagtttagta aatgcatatt ttgcatcaaa ttcacagttt gttccaggaa
      181 attgtagtac ggctactttc ataattaacc tactatatca atattgtaat cttcaattac
      241 agtattagca agaagttttt cacacatttt tgtaacttct tcttttgctt tttctttatc
      301 atttgagttt aactcaataa taatttgttt tccaactctt acatcagaaa ttaaatcttt
      361 aaatcccaaa gttcctaaag catggttaat tgctttacct tgatcatcta aaacaccttt
      421 ttttaatcct acatttacta ttgctttcat catttttcct ctaaaatttt ttgcaaatta
      481 tatctaaaga tttatttgct tagtctttct aaaattgttt tatatccatt taggatttta
      541 tcttctatct cttttggaac aacaacttct aatttctctc cagatttcaa agattttgat
      601 tgctctttcc agttctcttt tttaccaaaa tctcttaaaa tttgtttatc cattgagata
      661 aagttactgt ttttaaaatc ttctaaagct atgaatcttg aactctcagg agttaaaact
      721 tcatcaccta aaacccattt tccatcttca tcaacaaaaa tttcgaactt tgtatcaact
      781 acaactattc ctctatcttc tgcaaaagtt gtaaaatctt taaaaagctt ttctaaacta
      841 gagataattt caggaaaagt atctcttact tttttagagt ttaaaggctc gtcttttaag
      901 ccttttgttg ttggtgtaaa aagtggagtt tcaaatttat gccactcttt taaatttgaa
      961 ggtaaatcta atccatatgg atctttacca tcttgtaaag cttgaaataa agaaccagtt
     1021 aagtaatttc taaaaattag ttcaaactgt actacttccc catcaatatt tgcttcaaga
     1081 gctttgcaca gtttcataag ttgacatcta ggagcaacag agatatcaga agatattttt
     1141 tcatctaaaa ttgcagttct tataccactg tttgcagcaa attttgctcc aaaatttgaa
     1201 atttctgttt gtataattcc ttttccttta atttcaaaat ttaaaggaat atcaaaaaca
     1261 gatgttctat cacttcttac aagtaaaact tttgcctcat cacctggaca gctatataaa
     1321 tcagcatttt tccctatata aaaaagattg tagccaatat tttctaactc atcaaaacct
     1381 ttttgtgtag ttattttctt attctctggc caaagattta aagatttaat tttttcaata
     1441 ttcataaaac ttcctaaatt atttattcat tataattaag ctttttaaaa ctgctaaaga
     1501 tgtatttaat tgattgtcat tttgtaaatc atcttttgaa atgatttttt tagtttcatc
     1561 ttttacagct ttctcttcag cttttaactt atcatcaact ttattaagct caccctcaag
     1621 atgtttttta aggtcagcct cttttatttt taaaccattt tcctcacttt gaacaacttt
     1681 tccagcactt gcaataatat caggagttac accctctgct tggattgttc ttccacttgg
     1741 taaatagtat tttgcaatag ttaacttgat attttcagtt ttttcattat ctattggaag
     1801 tacagcttga accgaaccct ttccaaaagt tttttcacct actaaaatag ctcttttgtg
     1861 atcttgtaaa gcaccactta caatttcaga agctgaagca gaaccttcat ttacaagaac
     1921 aactaaaggt aaatctgttt ttgttccaaa tttacttgct tcaaattttt cttcatcaga
     1981 agcatctctt cctttttgtg aaacaataac tccatttttt acaaataggt caacaacacc
     2041 aattgcttga tttaaaagac caccaggatt atttcttaaa tcaagaacta taccttttac
     2101 atctttgttc tcttttatag ctttttctaa atcatttgta acttttgtgt caaagcttga
     2161 tattctaaga tataaaagat tttctccc
//
LOCUS       DAOQAN010000167         2178 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_73949_length_2178_cov_3.038154, whole genome shotgun sequence.
ACCESSION   DAOQAN010000167 DAOQAN010000000
VERSION     DAOQAN010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73949_length_2178_cov_3.038154"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..899
                     /locus_tag="PKO10_03430"
     CDS             <1..899
                     /locus_tag="PKO10_03430"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNW66235.1"
                     /translation="NITKRVYGRYKFLSEDVYFTIIGVDFSNLNKELKNLGLQNISKD
                     EMIVGFEVDNLLKKYKYTNYYDFFLPNKEIKKVKIAKVLEKESNIISSDIIILDINLA
                     RDILGINRDFSTNIAFDVPNELERANIKQKLQRLDLDLNIIQKEDILKKYETIFNYKG
                     GVFLILYLVVLFAFIMILYQRYSQVSINERKQIAIFKAIGYSVRDIIKIKMSENFVVA
                     FVSYLFGVLLAYFFVFILNAPILKNIFIDFSNIKNDFIIYPYIEFSTFVTLFLFFMVL
                     FLSSVLIPVWKISAINPYESLR"
     gene            896..1567
                     /locus_tag="PKO10_03435"
     CDS             896..1567
                     /locus_tag="PKO10_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNW66236.1"
                     /translation="MIVAKNISKIYFEGSKKEFYALKNINFEIKKGSFVIFKGVSGSG
                     KSTLLSILSTISKPSFGEILFDKEPVSKYPDIHASNFRAKNIGYIFQSFNLFEDLTVF
                     VNVSLPLIPLGFSQKDIDKKVEEILTLSNILHKKDEIVSNLSGGEKQRCAIARALINN
                     ANTILCDEPTSSLDYENSMNFVKTLKELKESGKTIVVATHDPLFFELDFVDKVFELKN
                     GVLVE"
     gene            1560..>2178
                     /locus_tag="PKO10_03440"
     CDS             1560..>2178
                     /locus_tag="PKO10_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66237.1"
                     /translation="MSSIFLSNEILIFLFLQTTIYTLLLISFIYSITILRDWDFKKST
                     ALQYKLEKRSYLVILIISFSLFIKILLFFYFIFSIDNLSHFVVGAMCAAGIFSLEYGE
                     ISLFLKLTNLFFIGIWFVLNSLDLKRKDYKYTKIKLLLFIFIFICLTFEYILDFKFLS
                     NIPLNEAVECCSIIFETSSISSKIPFGLVNSSLILIFYILFVLIVI"
BASE COUNT          812 a          212 c          288 g          866 t
ORIGIN      
        1 aaaatatcac aaagagagtt tacggaagat ataaattttt gagtgaagat gtttatttta
       61 ctattattgg agttgatttt tcaaatctaa ataaagagct aaaaaatttg ggacttcaaa
      121 atatatcaaa agatgagatg attgtaggtt ttgaagtaga taatttatta aaaaaataca
      181 aatatacaaa ttattatgat ttttttcttc caaataaaga gataaaaaaa gtaaaaattg
      241 caaaagtttt agaaaaagag tcaaatatta tatcaagcga tattattatt ttagatataa
      301 atcttgcaag agatattttg ggtataaaca gagatttttc aacaaatatt gcttttgatg
      361 ttccaaatga gcttgaaaga gcaaatataa aacaaaaact tcaaagatta gatttagacc
      421 tgaatattat tcaaaaagaa gatatcttaa aaaaatatga gacaatattt aactataaag
      481 gtggagtttt tttaatactt tatttagttg ttttatttgc atttataatg attttatatc
      541 aaagatactc tcaagtaagt ataaatgaga gaaaacagat agctattttt aaagcgattg
      601 gatatagcgt tcgagatatt ataaaaataa aaatgagtga aaactttgtt gtagcttttg
      661 tatcttacct ttttggagtc ttattagcat atttttttgt atttatttta aatgctccaa
      721 tattaaaaaa tatatttata gatttttcaa atataaaaaa tgattttata atctatccat
      781 atattgagtt ttctactttt gtaacacttt tcttattttt tatggttctg tttttaagtt
      841 cagttttaat tcctgtttgg aaaatatcag caataaatcc atatgaaagt ttaagatgat
      901 agtagcaaaa aatataagta aaatatattt tgaaggaagc aaaaaagagt tttatgcact
      961 aaaaaatata aattttgaga taaaaaaagg ctcatttgtt atatttaaag gtgtaagtgg
     1021 aagtggaaaa tcaacacttc tatctatact ttctactatt tcaaaaccta gctttggtga
     1081 aattcttttt gacaaagaac ctgtgtcaaa atatccagat attcatgcta gtaattttag
     1141 agcaaaaaat attggttaca tattccaatc ttttaatctt tttgaagatt taactgtttt
     1201 tgtaaatgtt agtcttcctc ttattccttt aggattttca caaaaagata ttgataaaaa
     1261 agtcgaagaa atattaactt tatcaaatat tttacataaa aaagatgaga tagtttcaaa
     1321 tctttctggt ggagaaaaac aaagatgtgc aattgcaaga gcattgataa acaatgctaa
     1381 tacaatttta tgtgatgaac caacatcttc tcttgattat gaaaactcta tgaattttgt
     1441 aaaaactcta aaagagttaa aagaaagtgg aaaaactata gttgtagcaa ctcatgaccc
     1501 actatttttt gagttagatt ttgtagataa ggtttttgag ttaaaaaatg gagttttagt
     1561 tgagtagtat ttttttatca aatgagatat taattttcct atttttacaa actactattt
     1621 atacactttt actaatatct tttatatatt caataactat tttaagagat tgggatttca
     1681 aaaaaagtac agctttacag tataaacttg aaaaaagatc atatttagtg attttaatca
     1741 tcagtttttc actttttata aaaattctac tcttttttta ttttatattt tctattgata
     1801 atttatcaca ttttgtagta ggtgctatgt gtgctgctgg tattttttct ttagaatatg
     1861 gtgaaatatc acttttttta aagcttacaa atctattttt tattggtatt tggtttgtct
     1921 taaactcttt agatttgaaa cgaaaagatt ataaatatac aaaaataaaa ttactacttt
     1981 ttatttttat attcatttgt cttacttttg agtatatttt agattttaaa tttttatcaa
     2041 atattccatt aaatgaagca gttgagtgct gttcaattat ctttgaaaca agtagtatat
     2101 ctagtaaaat accttttgga ttagttaatt caagcttgat acttatattt tatatacttt
     2161 ttgttttgat tgttattt
//
LOCUS       DAOQAN010000168         2169 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_74428_length_2169_cov_2.621097, whole genome shotgun sequence.
ACCESSION   DAOQAN010000168 DAOQAN010000000
VERSION     DAOQAN010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74428_length_2169_cov_2.621097"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            17..607
                     /locus_tag="PKO10_03445"
     CDS             17..607
                     /locus_tag="PKO10_03445"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNW66238.1"
                     /translation="MKATKILLSILLLTPFLYAKEFVVNSKNSKANFQLTYQKTNIVD
                     GSFQDISGLIIFDEKENIIKSIKGSVDTDSVSTQNGELTSLIISEKILNSKKYPEIEF
                     IAEKISDDKVFGDITINGVKRSVEFDIENSGIFLDKLYVTMSTTLKRSSFDLFWDVLE
                     NFGSSAVSNDIKVSIDIEATLQNDLIFQHAKEKTKK"
     gene            658..1116
                     /locus_tag="PKO10_03450"
     CDS             658..1116
                     /locus_tag="PKO10_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNW66239.1"
                     /translation="MKEKSLKTYGEKTDKSMKTVMRLLRVAHILNNKTELFLSKHTLT
                     FNQFKVLEVLYHKGDLNISSITKLIMGTPGNTTVVVKNLHRDGLIESKKDPNDNRSSI
                     LSITSKGSLIMQEIFPNHAKNLSEFLDVLDDYELKTLYDLLNKIYKEKKD"
     gene            1119..1670
                     /locus_tag="PKO10_03455"
     CDS             1119..1670
                     /locus_tag="PKO10_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013461073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNW66240.1"
                     /translation="MKKITLGLLSIFTASSLFAGTYSVDTSHSTAGFSVKHMMVSNVV
                     GKIKDISGTYEYDEKANTLLSVQGELNVASIDTADEKRDAHLKADDILDVAKFPKISF
                     KSTKVEKDVVYGDLTIKGITKNIKLNLENGGSLGKKSGFSLTGKINRSEFGVTWNKIL
                     ETGGVAVSDEVKLNIDIEGNLTN"
     gene            1689..>2169
                     /locus_tag="PKO10_03460"
     CDS             1689..>2169
                     /locus_tag="PKO10_03460"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HNW66241.1"
                     /translation="MNPSLFISHGAPNIIFSDLKSKKSIQNLKNILDTPKYIIIVSAH
                     YVNKNLKVINPIANSLMYDFYGFEKELYEFKYNIKSDINLTNNLIEKLKNQGIDISID
                     ENRTSYDHGVWSVLSLIYEKLEIPVIQLSIPISYSINELLNLGEKLKIFKNEALLIFS
                     "
BASE COUNT          912 a          217 c          314 g          726 t
ORIGIN      
        1 ttttataagg taaaaaatga aagcaacaaa aatcttatta tcaatactat tattaactcc
       61 ttttctttat gcaaaagagt ttgtcgtaaa tagtaaaaac tcaaaagcaa attttcaact
      121 aacataccaa aaaacaaata tagttgatgg aagctttcaa gatattagtg gattgataat
      181 ttttgatgaa aaagaaaaca taataaaatc aataaaaggt tctgtggata cagactcagt
      241 atcaacacaa aatggtgaat taacatcttt aataataagt gaaaaaatct taaatagcaa
      301 aaaataccca gaaattgaat ttattgctga aaaaataagt gatgataaag tttttggtga
      361 tataacaata aatggtgtaa aaagaagtgt cgaatttgat attgaaaata gtggcatctt
      421 tcttgataaa ctttatgtga caatgagtac aactttaaaa agaagttctt ttgatctctt
      481 ttgggatgtt ttagaaaatt ttggaagtag tgctgtatca aatgatatta aagttagcat
      541 agatatcgaa gcaactttac aaaatgattt aatttttcaa catgcaaaag agaaaacaaa
      601 aaaatagtat tgacttttaa tactaattag taatatactt tcacctaagg agtctttatg
      661 aaagaaaaaa gtttaaaaac ttatggtgaa aaaactgata aatcaatgaa aacagttatg
      721 aggctcttaa gagttgctca cattttaaat aacaagactg aacttttttt atcaaaacat
      781 actcttacat ttaatcaatt taaagtttta gaagttctat accacaaagg tgatttaaat
      841 ataagttcaa ttacaaaact tattatggga actcctggaa atacaactgt tgttgttaaa
      901 aatttacata gagatggact aattgagtca aaaaaagacc caaatgacaa tagatcttca
      961 attttatcaa taacttcaaa aggaagtttg ataatgcaag agatttttcc aaatcatgca
     1021 aaaaatctaa gtgagttttt agatgtttta gatgattatg aattaaaaac tttatatgat
     1081 ttattaaata aaatttacaa agaaaaaaag gattaaaaat gaaaaagatt actttaggat
     1141 tattaagtat atttacagca agctcgttat ttgctggaac ttatagtgta gatacatcac
     1201 attcaactgc tggtttttca gtaaaacata tgatggtttc aaatgttgtt ggaaaaataa
     1261 aagatatatc tggcacttat gaatatgatg aaaaagcaaa tactttatta agtgttcaag
     1321 gtgaattaaa tgttgcttct attgatacag ctgatgaaaa aagagatgct catctaaaag
     1381 ctgatgatat tttagatgtt gcaaaattcc caaaaataag ttttaaatct acaaaagtag
     1441 aaaaagatgt agtttatggt gatttaacta taaaaggtat tacaaaaaat attaaattaa
     1501 atcttgaaaa tggtggttca ctaggtaaaa aatcaggttt ttcactaact ggaaaaataa
     1561 atagaagtga atttggtgta acttggaata aaattttaga aacaggtgga gttgctgtta
     1621 gtgatgaagt aaaattaaat attgatattg aaggaaattt gactaattaa ttttagtcaa
     1681 ataatattat gaatccatct ttatttatct ctcatggagc acctaatatt atttttagtg
     1741 atttaaaatc aaaaaaaagt attcaaaatc ttaaaaatat tttggatact ccaaaatata
     1801 ttattatagt atcagcgcac tatgtaaata aaaatttaaa agttataaat cctattgcaa
     1861 atagtttaat gtatgatttt tatggttttg aaaaagagtt atatgagttt aaatacaata
     1921 taaaaagtga tataaattta acaaataatc taattgagaa attaaaaaat caaggtattg
     1981 atataagtat agatgaaaac cgaactagtt atgaccacgg agtttggagt gttttatctt
     2041 taatatatga aaaacttgaa attccagtaa ttcaacttag tattccaata tcttatagca
     2101 tcaatgaact attaaactta ggtgaaaaac taaaaatatt taaaaatgaa gctttactta
     2161 tttttagtg
//
LOCUS       DAOQAN010000169         2168 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_74464_length_2168_cov_3.489825, whole genome shotgun sequence.
ACCESSION   DAOQAN010000169 DAOQAN010000000
VERSION     DAOQAN010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74464_length_2168_cov_3.489825"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..844
                     /locus_tag="PKO10_03465"
     CDS             <1..844
                     /locus_tag="PKO10_03465"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNW66242.1"
                     /translation="LIDNEMFNLLSTNAKQKVLKLNENYNRLTENTKANQQEINKLNS
                     YIKDRLKTKENYKALQIKHTKSLENLEKEHKLYQAELKKVIEQQESLSKILSDLKIVK
                     QQELKKAQEEKEDNQQVQTTNVRNQKYAKDLDLDVKKIGSSTDGVQIVKYKGAKTIAP
                     LKSFKIVKNFGTYYDPVYKIKLFNESVVLQSNEKGSKVVAVLNGKVVYAKKNAGMLEN
                     VVIIQHEGGIHTVYSHLDDIAPTLVVGKWVQKGSVVGRVDENLTFQVTKDSSHIDPKD
                     LFNI"
     gene            853..1281
                     /locus_tag="PKO10_03470"
     CDS             853..1281
                     /locus_tag="PKO10_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66243.1"
                     /translation="MVKNSIDNPVFLIFLLIFAVSINFLASTHFLVILFAGVISTAFY
                     RTLKQKYYYSFAFVIIAFLLIELNMGLKPFSLSLLSYFVYLFIIPRYEQNQINTFLYV
                     IFFYIGLGIIWYIFFNFDSFFSYILIINLIIDLILVGIFL"
     gene            1278..>2168
                     /locus_tag="PKO10_03475"
     CDS             1278..>2168
                     /locus_tag="PKO10_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNW66244.1"
                     /translation="MNFRLTLIYIFIGIIAITLLSRVYFLSIKSNTYYEELSKNNYIK
                     KTYLAPTRGVIEDRNGEPLALNQIGFSILIKPHLRAPKYREKLEKLIDIVVEQFPQYD
                     KNKLIKDYLREDSPYNHEYIKVIDYVPYEEIFSRYTFLATQEDIRIESSSKRHYPHKE
                     LAAHIIGYVSKASKLDIANNEIAKYSGVVGKSGIERYYNDKLQGTLGFKDTKVNALNK
                     EIEVIEQKDPSNDNNVRITIDVNLQKYIQELFVDRAGSVIVMDANNGEILAAGSFPEY
                     DSNLFARGITQEEWNLMRNDF"
BASE COUNT          885 a          248 c          306 g          729 t
ORIGIN      
        1 gctaatagac aatgaaatgt tcaatcttct atcaacaaat gcaaaacaaa aagttttaaa
       61 actaaatgaa aattataata gactaacaga aaatacaaaa gcaaatcaac aagagataaa
      121 taaactaaat agctatatca aagatagatt aaaaactaaa gagaactata aagctctaca
      181 aataaagcat acaaaatctt tagagaactt agaaaaagag cataaacttt atcaagctga
      241 acttaaaaaa gttattgaac aacaagagtc tttgagtaaa atattatctg acttaaaaat
      301 tgttaaacaa caagagttaa aaaaagcaca agaagagaaa gaagataatc aacaagtaca
      361 aacaacaaat gtacgaaatc aaaaatatgc aaaagattta gatcttgatg ttaaaaaaat
      421 tggttcttca acagatggtg ttcaaatagt aaaatacaaa ggtgcaaaaa ctatagctcc
      481 tttaaaatca tttaaaattg ttaaaaattt tggaacatat tatgaccctg tttataaaat
      541 aaaattattt aatgaatctg ttgttttaca atcaaatgaa aaaggttcaa aagtagtagc
      601 tgttctaaat ggaaaagttg tatatgcaaa aaagaatgct ggaatgctag aaaatgttgt
      661 aattattcaa catgaaggtg gaattcatac tgtttactct cacttagatg atattgcacc
      721 aactttagtt gtaggaaaat gggttcaaaa aggctcagtt gtaggaagag tagatgagaa
      781 cttaactttt caagttacaa aagatagctc tcatatagat ccaaaagatc tctttaatat
      841 ttaggtaatg ttatggtaaa aaatagcata gacaatccag tttttttaat atttttacta
      901 atttttgcag tgagtataaa ttttttagct tctactcatt ttttagttat tttatttgca
      961 ggagttattt caactgcatt ttatagaact ttaaagcaaa aatattacta ctcttttgcg
     1021 tttgttataa ttgctttttt acttattgag ttaaatatgg gacttaaacc attttcctta
     1081 tcattactat catattttgt atatcttttt ataattccaa gatatgaaca aaatcagata
     1141 aatacatttt tatatgtaat ctttttttac attggcttag gtattatttg gtatatcttc
     1201 tttaattttg actcattttt tagttatatt ctaataataa atcttattat agatttaatc
     1261 ttagttggga tatttttatg aattttagat taactcttat atatattttt attggaatta
     1321 ttgctattac gctactttca agagtatatt ttttatctat caaatcaaac acttactatg
     1381 aagagttatc aaaaaacaat tatataaaaa aaacatattt agcacctaca agaggtgtaa
     1441 tagaagatag aaatggagag cctttggctc taaatcaaat aggattttct attcttataa
     1501 aacctcattt aagagctcct aaatatagag aaaaactaga aaaacttata gatattgtag
     1561 tagaacaatt tccacaatat gataaaaata aattaataaa agattattta agagaagatt
     1621 ctccttacaa tcatgaatat ataaaagtta ttgattatgt tccttatgaa gagatttttt
     1681 caagatatac atttttagca actcaagagg atattagaat tgaatcatct tcaaaacgac
     1741 actatcctca taaagagcta gctgcacata ttataggtta tgtaagtaaa gcttcaaaac
     1801 ttgatattgc aaacaatgaa atagcaaaat atagtggtgt tgtaggtaaa agtggaattg
     1861 agagatacta taatgacaaa cttcaaggaa cactaggatt taaagataca aaagtaaatg
     1921 ctttaaataa agagattgaa gtaatagaac aaaaagatcc ttcaaatgac aataatgtaa
     1981 gaattacaat tgatgttaat cttcaaaaat atattcaaga actgtttgta gatagagctg
     2041 gttctgttat agttatggat gcaaataatg gtgaaatatt agcagcaggt tcatttcctg
     2101 agtatgatag taatcttttt gcaagaggaa ttactcaaga agagtggaat cttatgagaa
     2161 atgatttt
//
LOCUS       DAOQAN010000170         2159 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_74936_length_2159_cov_2.990494, whole genome shotgun sequence.
ACCESSION   DAOQAN010000170 DAOQAN010000000
VERSION     DAOQAN010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74936_length_2159_cov_2.990494"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1013
                     /gene="tolB"
                     /locus_tag="PKO10_03480"
     CDS             <1..1013
                     /gene="tolB"
                     /locus_tag="PKO10_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HNW66245.1"
                     /translation="IKTAFLKALSNLSAKKDGNGNYTLMTKLYDINSRALVLEKNYTT
                     SLEERFVFLAHKAAISINDHFKAPSISWMDRFVVFSVYEGPGKADIMIGDYTLTYKKR
                     VVSGGLNIFPKWADKEQKTIYYTSYNYKKPTLVKLNIYNRSKDVIMDSDGMLACSDVN
                     ADGTKLLVTASPAGQPDVFLYDTRTRAKKQITTYSGIDVGGQFVENDSKIVFVSDRLG
                     NPNIFAQTIGSSGVERLVFHSNNNSSVTSSGNNVIFSSKDASNELGKSFNLYLISTKS
                     DSLKRLTSSGVNQFPKFSSDGQSVLFIKTDGTSSVGIIRLEHNKSFLFPLAGKRIQSI
                     DW"
     gene            1193..1714
                     /locus_tag="PKO10_03485"
     CDS             1193..1714
                     /locus_tag="PKO10_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNW66246.1"
                     /translation="MKKLGLYSVLVSALLFTTGCSEKNADVNDVVAPDSSSVEAPIQD
                     GTLLERAGNGNYYMINGQRVLIEHVYFDFDKYNLTPANKDKAVSNASKLSQVSSDTTI
                     KVFGNTDEWGSDEYNYALGLKRANSVKDVLVANGVKTNVTMVSLGESNPVCTEKTKEC
                     WAENRRVEHELEK"
     gene            1720..>2159
                     /locus_tag="PKO10_03490"
     CDS             1720..>2159
                     /locus_tag="PKO10_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014469056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66247.1"
                     /translation="MKKIIPLLFVITSLTVAEEVSVYGASNQDSSYGLSATEKHILKN
                     QSNISNLSSKLEEINSLVKSINGRLEGLESTYEGDSKKLNDLSLKLNQSSDLASNEQA
                     SNVNQSDLNSLKAALTKLTTMVNKINAEYVSSTELEKNMQQFIT"
BASE COUNT          835 a          250 c          359 g          715 t
ORIGIN      
        1 aaattaaaac agctttttta aaggctttgt caaatctttc agcaaaaaaa gatggaaatg
       61 gtaactatac tttaatgaca aagttgtatg atataaattc aagagctttg gttttggaaa
      121 aaaattatac aacttcactt gaggagagat ttgttttttt agcacataaa gcagctattt
      181 ctataaatga ccattttaaa gctcctagta tatcttggat ggatagattt gtagtatttt
      241 cagtttatga aggtccagga aaagctgata ttatgatagg tgattatact ttaacttata
      301 aaaaaagagt tgtaagcggt ggtcttaata tatttccaaa atgggcagat aaagagcaaa
      361 aaactattta ctatacatct tataactata aaaaaccaac tttggttaag ttaaatatat
      421 ataatagaag taaagatgta attatggatt ctgatggaat gttggcttgt tcagatgtaa
      481 atgcggatgg aacgaaactt ttagtaacag cttctccagc aggtcaacca gatgtatttt
      541 tgtatgacac aagaacaaga gcaaaaaaac agataacaac ttatagtgga attgatgttg
      601 gtggacaatt tgttgaaaat gatagtaaaa tagtttttgt atcagataga ttaggaaatc
      661 caaatatttt tgctcaaact attggatcaa gtggtgttga aagattagtt tttcatagca
      721 acaacaactc atcagttact tcaagtggaa ataatgtaat ttttagtagt aaagatgcaa
      781 gtaatgagtt gggtaaaagt tttaatctat atcttatttc aacaaaatca gatagtttaa
      841 aaagattaac ttcaagtggt gtaaatcagt ttccgaaatt ttctagtgat ggacaaagtg
      901 ttttatttat aaaaactgat ggaactagtt ctgttggtat cattagatta gagcacaata
      961 aatcgttttt attcccattg gcaggaaaaa gaattcagtc aattgactgg taaaatttca
     1021 aaaacatttt agggctatat gccctaaaat atcaaatctc ttttaaattt acttttttta
     1081 ttaaaaaaag attcaaatca tataaaatat tataaaaaag acaaaaaata taaacaataa
     1141 ttaagtttca ctttagtact atttcttgaa aattttattt taaggaaaaa taatgaaaaa
     1201 gttaggtctt tattcagttt tagtttcggc tttactattt actacaggtt gtagcgaaaa
     1261 aaatgctgat gtaaatgatg ttgtagcacc agatagctca tctgttgagg caccaatcca
     1321 agatggtact cttttagaaa gagctggtaa tggaaactat tatatgatta atggtcaaag
     1381 agttttaatt gagcatgttt attttgattt tgataaatac aatttaacac ctgctaataa
     1441 agacaaggct gtaagcaatg cttctaaact ttcacaagtt tcttctgata ctacaattaa
     1501 agtattcgga aacactgacg aatggggaag tgatgagtac aattatgctt taggtttaaa
     1561 aagagcaaat tctgttaaag atgttttagt agcaaatggt gtaaaaacta atgttacaat
     1621 ggtatcttta ggtgagagta atccagtttg tactgaaaaa actaaagagt gttgggcaga
     1681 aaacagaaga gttgagcacg aattagaaaa ataattttta tgaaaaaaat tataccttta
     1741 ttattcgtaa taacttcatt aaccgtagcc gaagaggttt cggtttatgg agcatctaac
     1801 caagatagct catatggatt atctgcaact gaaaaacata tactaaaaaa tcaatcaaat
     1861 ataagtaatt tatcttctaa acttgaagaa attaatagtt tagtaaaatc tattaatggt
     1921 agattagagg gcttagagtc tacatatgaa ggtgattcaa aaaaattaaa tgatttatct
     1981 ttaaaattaa atcaatcttc agatttagct tctaacgagc aagcgtcaaa tgtaaatcaa
     2041 tcagatttaa atagtttaaa agcagctctt acaaaattaa caactatggt taataaaata
     2101 aatgcagaat atgtttcatc gacagaatta gaaaaaaata tgcaacaatt tattactag
//
LOCUS       DAOQAN010000171         2152 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_75296_length_2152_cov_2.569862, whole genome shotgun sequence.
ACCESSION   DAOQAN010000171 DAOQAN010000000
VERSION     DAOQAN010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75296_length_2152_cov_2.569862"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="PKO10_03495"
     CDS             <1..309
                     /locus_tag="PKO10_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HNW66248.1"
                     /translation="PNYNDKSLKIVQEYQEIMKKYSNESSLGFISFEAFLASKVLVNQ
                     ISKIEELGELNRKNLLNALKSPQKNILYELELDFKNNQLLNKTYLFEYENGNFKELIR
                     "
     gene            306..1415
                     /locus_tag="PKO10_03500"
     CDS             306..1415
                     /locus_tag="PKO10_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNW66249.1"
                     /translation="MKRVFNYFNNLKFSYKANFLIFIIAGGMFTIIILSQLSIFILKN
                     DFDTLFEKRTKTLIKLENINDSYKINIQNTLSEFEKNNLNYEQTLEVLKIANEIIDKN
                     WSEYKNNSYSTKTDFVINYIQKHFSNKNYYKNELIKNLNIQNVDEKMNKILHYLNNIK
                     EKNDKEYFTNLNFEIDAILIYLGSLVNYDLNLAINEKRDTDNIFHLITIFSFISIFLV
                     VLFTIILSLFITVHFRRIHDLQEKIVEDKTKELKELNANLELKISQEVSKNRKKDIIM
                     FQQARFASLGEMLNNIAHQWRQPLGSISMIIQSFQTKMRLNKLSLDFVEQKVADALLL
                     AQNMSNTLDDFKNFFSPNKSKNSFFIKDFLKNKIK"
     gene            1461..1886
                     /locus_tag="PKO10_03505"
     CDS             1461..1886
                     /locus_tag="PKO10_03505"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNW66250.1"
                     /translation="MKKNVKIYSFENELSHVFLNIISNSKDALINNISKDNRVIRVIV
                     NSKKDFVFVNISDNGGGIPPLIMPKIFEPYYTTKYKSAGTGIGLYMSKQIIEKHIKGQ
                     ISCKNIEIIINNQKYDGTSFLIKIPINKEIIEEKKEKDE"
     gene            1879..>2152
                     /locus_tag="PKO10_03510"
     CDS             1879..>2152
                     /locus_tag="PKO10_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNW66251.1"
                     /translation="MNKNLDILKNINILYLEDDENLLKHTSDILEDFVANIYGVKNTI
                     DAMKILLEKKVDVIISDILLENENGINFLKYIKSKDILIPAILTTAH"
BASE COUNT          983 a          194 c          258 g          717 t
ORIGIN      
        1 ccaaactaca acgataaatc tttaaaaata gtacaagagt atcaagaaat tatgaaaaaa
       61 tattcaaacg agagcagttt gggatttata tcatttgaag catttttagc ttcaaaggtt
      121 ttggtaaatc aaatatcaaa aattgaagag ttaggagagt taaatagaaa aaatctatta
      181 aatgctttaa aaagtccaca aaaaaatatt ttgtatgaat tagagttaga ttttaaaaac
      241 aatcaacttc taaataaaac atatcttttt gagtatgaaa atggaaattt taaagagctt
      301 ataagatgaa aagagttttc aactatttta ataacttaaa attttcatac aaagcaaatt
      361 ttttaatatt tataattgct ggtggaatgt ttactattat catactatct caattatcca
      421 tttttatttt aaaaaatgat tttgatacac tttttgaaaa acgaacaaaa actctaataa
      481 aactcgaaaa tataaatgat tcatataaaa taaatattca aaatacttta agtgaatttg
      541 aaaaaaacaa tctaaactat gaacaaactt tagaagtttt aaaaatagca aatgagataa
      601 tagataaaaa ttggagtgaa tataaaaata actcatattc tacaaaaact gattttgtaa
      661 taaattatat acaaaaacat ttttcaaata aaaactacta taaaaacgag ttaataaaaa
      721 atttaaatat ccaaaatgtt gatgaaaaaa tgaataaaat attgcactat ttaaataata
      781 taaaagagaa aaacgataaa gagtacttta caaatttaaa ttttgaaata gatgctattt
      841 tgatatattt agggagttta gtaaattatg atttaaattt agcaataaat gaaaaaagag
      901 acacagataa tatctttcat cttataacaa ttttttcttt tatctctatt tttttagttg
      961 ttttatttac aataatttta tctttattta taactgtaca ttttagaaga atacatgatt
     1021 tacaagaaaa aattgtagaa gataaaacca aagagctaaa agagttaaat gcaaatttag
     1081 aattaaaaat atctcaagag gtttcaaaaa ataggaaaaa agatattatt atgtttcagc
     1141 aagcaagatt tgcaagctta ggagagatgt taaataatat agcacatcaa tggagacaac
     1201 ctttaggttc tatatctatg attatccaaa gttttcaaac aaaaatgaga ctaaataagc
     1261 tttcactaga ttttgtagaa caaaaagttg cagatgcact acttttagca caaaatatgt
     1321 caaatacact tgatgatttt aaaaattttt tcagtccaaa taaatcaaaa aatagctttt
     1381 ttataaaaga ttttttaaaa aataaaataa aatgaaaaaa atatttttta aataaagaga
     1441 atataaaagt agatttaatt gtgaaaaaaa atgtaaaaat atatagcttt gaaaatgagt
     1501 tatctcatgt ttttttaaat ataatatcaa attcaaaaga tgctttaata aacaatatat
     1561 ctaaagataa tagagtaata agagttattg taaatagtaa aaaagatttt gtttttgtaa
     1621 atattagtga taatggaggc ggaattcccc cacttattat gccaaaaata tttgaaccat
     1681 attacacaac aaaatataaa agtgcaggaa ctggaattgg actttatatg tcaaagcaga
     1741 ttattgaaaa acatataaaa gggcaaattt cttgtaaaaa tatagaaatt attataaata
     1801 atcaaaaata tgatggaaca tcatttttga taaaaatacc aataaataaa gagattattg
     1861 aagaaaaaaa ggaaaaagat gaataaaaat ctagatattt taaaaaatat taatatttta
     1921 tatttggaag atgatgagaa ccttttaaaa catactagtg atattttaga agattttgta
     1981 gcaaatattt atggtgtaaa aaacactatt gatgctatga aaattttact tgaaaaaaaa
     2041 gtagatgtaa taataagtga tattttgctt gaaaatgaaa atggtataaa ctttttaaaa
     2101 tatattaaaa gtaaagatat acttatacct gcaattttaa ctaccgcaca ta
//
LOCUS       DAOQAN010000172         2142 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_75852_length_2142_cov_2.544801, whole genome shotgun sequence.
ACCESSION   DAOQAN010000172 DAOQAN010000000
VERSION     DAOQAN010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75852_length_2142_cov_2.544801"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..1365)
                     /locus_tag="PKO10_03515"
     CDS             complement(79..1365)
                     /locus_tag="PKO10_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HNW66252.1"
                     /translation="MPTVLEIFKEITKIQRCSGNHKDFIEYMKEISKKLEYICLVDEA
                     NNILCKKEDSNANIVFQSHYDIVCLNDFCIPQIVEDDTTLKAIDSTLGADNGIGCSYM
                     IALMYENFDGEFLFTSDEEIGLIGANGLNLPINSKYMLNLDSEDEGKICIGCAGGVDI
                     IAKNSNKKIVPNIENLDLYEISISNLQGGHSGVDIDKNIPNAIKLIVKTIKECEGKIL
                     DINGGERINSIPANTKAIIASKKRPQKTHENMKIEKINTKSEHLNIYDDEIINFLYNF
                     QNGVRDFNKELNVVQDSINLALIKTNIDEIVIEFSARSMNNENLKELKEKTKKDLINN
                     NFYVETYGKYPAWSPDINDFTSKVLDIYKTFDKNASLEAIHAGLECAIFKDKFPNLKI
                     ASIGPTIKFPHSKKEFVYKKSIENVFEVVKKIANSI"
     gene            complement(1476..>2142)
                     /gene="ccsA"
                     /locus_tag="PKO10_03520"
     CDS             complement(1476..>2142)
                     /gene="ccsA"
                     /locus_tag="PKO10_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HNW66253.1"
                     /translation="IFFRNSLLALGAATIIAGIFMFTAHLTDVDPQITSLVPVLKSYW
                     LTIHVSILTASYGFFGLSAILGFLTLIMFIFRKNRPHLDDIIKHISAINEISLIIGLA
                     CITIGNFLGGVWANESWGRYWGWDPKETWAYVSIVIYALVIHLRFVKSLNTPYVLATA
                     SLLAFSSIMMTYLGVNFYLSGMHSYATGDPVPIPMWAYLTVATAFAAIILAYKNRDLK
                     NID"
BASE COUNT          769 a          329 c          237 g          807 t
ORIGIN      
        1 ataaaagata gtgatgaatg ggaaaatttt taaactctta aaaaataaaa gttgagaatt
       61 aatctcaact tttaaagact atatactatt tgcaattttt ttaactactt caaaaacatt
      121 ttcaatagat tttttatata caaactcttt ttttgagtga ggaaatttta tagttggacc
      181 aatagaagct atttttaaat ttggaaattt atctttaaaa atagcacact ctaatccagc
      241 atgaatagct tctaaggatg catttttatc aaaagttttg taaatatcca aaacttttga
      301 tgtgaaatca tttatatcag gactccaagc tggatatttt ccataagttt ctacataaaa
      361 attgttattt attaaatctt tttttgtttt ctcttttaac tcttttaaat tttcattatt
      421 cattgatcta gcactaaatt caataacaat ttcatctata tttgtcttaa ttaatgctaa
      481 atttatagag tcttgaacta catttaactc tttattaaaa tctcttacac cattttgaaa
      541 gttatataaa aaattaataa tttcatcatc ataaatattt aaatgttcag attttgtgtt
      601 aattttttct attttcatat tttcatgagt tttttgaggt ctcttttttg aagcaattat
      661 agcttttgta tttgctggaa ttgagtttat tctctcacct ccatttatat ccaatatttt
      721 tccttcacac tcttttatag tttttacaat tagttttata gcatttggaa tatttttatc
      781 tatatcaaca ccactgtgtc caccttgtaa atttgatata gaaatttcat ataaatctaa
      841 attttcaata tttggaacta tttttttgtt tgagtttttc gcaataatat ctactcctcc
      901 agcacaacca atacaaattt taccttcatc ttcgctatca agatttagca tatatttaga
      961 atttatagga agatttaaac catttgcacc tatgagtcct atctcttcat cacttgtaaa
     1021 taaaaattct ccatcaaaat tttcatacat taaagctatc atatatgagc aaccaattcc
     1081 attatcagct cccaaagttg aatctatcgc ttttaaagtt gtatcatctt caacaatttg
     1141 aggaatacaa aaatcattta aacaaacaat atcatagtga gattgaaata ctatatttgc
     1201 gtttgagtcc tcttttttac ataaaatatt attggcttca tcaactagac aaatatattc
     1261 tagtttttta gatatttctt tcatatattc tataaaatct ttatgatttc cactacatct
     1321 ttgtattttt gtaatctctt taaatatttc taaaacagtt ggcattgata tccttacttt
     1381 ataaaacttg aaaaaaagca attttaagaa aaaagtatat ttgctttttt acaaatttat
     1441 atcttaaata aaaaaagggc taaaagccct tttatctaat ctatattttt taaatctcta
     1501 tttttgtaag ctaatataat tgcagcaaaa gcagttgcaa cagttaaata agcccacata
     1561 ggaataggta caggatcacc tgttgcataa gagtgcattc cagataagta gaagtttact
     1621 cctaaataag tcatcataat agagctaaat gcaagtaatg aagcagttgc taaaacatat
     1681 ggtgtattta atgattttac aaatctaagg tgtataacta aggcataaat aactattgaa
     1741 acatatgccc aagtctcttt tggatcccat ccccagtatc taccccaaga ctcatttgcc
     1801 caaactccac caaggaaatt tccaatagta atacatgcta aaccaataat taaagaaatc
     1861 tcatttattg cacttatgtg tttaatgatg tcatccaaat gtggtctatt ttttctaaaa
     1921 ataaacataa ttaaagttaa gaatcctaaa atagcactaa gtccaaagaa tccgtatgaa
     1981 gcagtcaaaa tagatacatg gattgtaagc caataagatt ttaaaacagg caccaaactt
     2041 gtaatttgag gatcaacatc tgttaagtga gccgtaaaca taaaaatacc agcaataatt
     2101 gtagcagcac ccaaagctaa taaagagttt ctaaaaaata ta
//
LOCUS       DAOQAN010000173         2130 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_76520_length_2130_cov_3.075663, whole genome shotgun sequence.
ACCESSION   DAOQAN010000173 DAOQAN010000000
VERSION     DAOQAN010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76520_length_2130_cov_3.075663"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="PKO10_03525"
     CDS             <1..254
                     /locus_tag="PKO10_03525"
                     /inference="COORDINATES: protein motif:HMM:NF026774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="plasminogen-binding N-terminal domain-containing
                     protein"
                     /protein_id="HNW66254.1"
                     /translation="IFIVLNRKIFIYDAKTFTLLDSYSLQIGSSKKQLPFFTRVEEIK
                     GPLIDLKNIPIVSDVLGLNEDGIDDSSYDNYYKQLLGVK"
     gene            254..1642
                     /locus_tag="PKO10_03530"
     CDS             254..1642
                     /locus_tag="PKO10_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HNW66255.1"
                     /translation="MIDKKHLEYITSIVGDENIKTDKAHLIAFCYDATRSRFEPDAVV
                     FPRHEQDVSDILKYCNEHKIVIVPRGAGSGFTGGALPSNGGIILSLERHMNKILEIDM
                     QNMVGVVQPGVINMQFQKTVEEVGLFYPPDPASEEYSTLGGNVSENAGGMRAAKYGIT
                     KDYVMALRAVLPNGDIIVAGKKTIKDVAGYNVAGILIASEGTLAVITEITLKLIPKPK
                     FKKTYMGVFPNVNSAMTAVFKSLASGANPVAMEFLDSLVIKALKQKFPQVSLPENAGG
                     ILIGDVDASREAEIEDQLNILKESFKENGSIDFIVAQNEEEGKKLWFARRNASPATMV
                     YGTKKLNEDISVPRSKLPEALDEIYKIGEKYGFQVPCFGHAGDGNIHVNVMVKDKTNE
                     KEMADGHKAIEEIFQLVVDMGGTLSGEHGIGISKAPFMNIAFTDAEMNLFRSIKKAFD
                     PNNILNPFKMGL"
     gene            1644..>2130
                     /locus_tag="PKO10_03535"
     CDS             1644..>2130
                     /locus_tag="PKO10_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013136103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HNW66256.1"
                     /translation="MENSKNTISKKLFVAVETFFNDDTSYYAASLSFFTIFSILPIIA
                     LGIAIISQFQEFNSYLDMFTNFLLNFLNPTHSDEIVNTLKNFISNSDKLGFVGIFYML
                     FVYIMFFKDYDYIVNKIHQTTRREIYKSFFIYTIFFIVFPAIFIVLNLLLSFYDSNLF
                     KK"
BASE COUNT          786 a          254 c          370 g          720 t
ORIGIN      
        1 ctatttttat tgtattaaat agaaaaatat ttatctatga tgcaaaaaca tttactctac
       61 tagatagtta tagtttacaa attggttctt ctaaaaaaca gttaccattc tttacaagag
      121 ttgaagagat aaaaggacca ttaattgatc ttaaaaatat tcctatagta tcagatgtat
      181 taggtttaaa tgaagatggt atagatgatt caagttatga caactactat aagcaacttt
      241 tgggagttaa atagtgattg ataaaaaaca tttagagtac ataacttcta ttgtaggaga
      301 tgagaatatc aaaactgata aagctcatct aatagctttt tgctacgatg ctacaagaag
      361 ccgttttgag ccagatgctg ttgtttttcc acgacatgaa caagatgtaa gtgatatttt
      421 aaaatattgt aatgaacaca aaattgtaat tgtaccaaga ggtgctggaa gtggatttac
      481 aggtggtgca ttaccttcaa atggaggaat tattctatca cttgaaagac atatgaataa
      541 gatacttgaa attgatatgc aaaatatggt tggagttgtt caaccaggag ttataaatat
      601 gcagtttcaa aaaacagttg aagaagttgg tttattttat cctccagatc cagcaagtga
      661 agagtattca acacttggtg gaaatgtaag tgaaaatgca ggtggaatga gagctgctaa
      721 atatggtatt acaaaagatt atgtaatggc actaagagca gtgcttccaa atggtgatat
      781 tatcgtagct ggtaaaaaaa ctatcaaaga tgttgctggt tataatgttg ctggtatttt
      841 aatagctagc gaaggaactt tggcagttat tactgaaatt actttaaaac ttatcccaaa
      901 accaaaattc aaaaaaactt acatgggagt ttttccaaat gtaaatagtg ctatgacagc
      961 tgtatttaaa tcgcttgcaa gtggtgcaaa tcctgttgct atggagtttt tagactcact
     1021 tgtaataaaa gctttaaaac aaaagtttcc tcaagtttca ttaccagaaa atgctggtgg
     1081 aattttaatt ggagatgtag atgcaagtag agaagctgaa atagaagatc agctaaatat
     1141 tttaaaagag tcatttaaag agaatggctc aattgatttt attgtggctc aaaatgaaga
     1201 agaaggaaaa aaactatggt ttgcaagacg aaatgcaagt ccagcaacta tggtttatgg
     1261 aactaaaaaa ttaaatgaag atataagtgt tccaagaagt aagcttccag aggctttaga
     1321 tgagatttat aaaattggtg aaaaatatgg tttccaagtt ccttgttttg gacatgctgg
     1381 agatggaaat attcacgtaa atgttatggt aaaagataaa acaaatgaaa aagagatggc
     1441 tgatggtcat aaagctattg aagagatttt ccaactagtt gtagatatgg gtggaacttt
     1501 aagtggtgaa cacggtattg gtatatcaaa agcaccattt atgaatattg cttttacaga
     1561 tgctgaaatg aatttattta gaagtatcaa aaaagctttt gacccaaaca atattttaaa
     1621 tccatttaaa atggggttat aaattggaaa atagtaaaaa tactatttcc aaaaagctat
     1681 ttgtagcagt tgaaacattt tttaatgatg acacaagcta ctatgctgca agtcttagtt
     1741 tctttactat attttcaata ctaccaataa ttgctcttgg aattgctatt atttcacaat
     1801 ttcaagagtt taactcatat cttgatatgt ttacaaattt tttactaaat tttctaaacc
     1861 ctacacactc tgatgaaatt gtaaatactc taaaaaattt tatatcaaac tctgataaat
     1921 taggatttgt aggaatattt tatatgcttt ttgtatatat tatgtttttc aaagattatg
     1981 actatattgt aaataaaata catcaaacaa caagaagaga aatatataaa tcatttttta
     2041 tatacacaat cttttttata gtgtttcctg ctatttttat tgttttaaat ttacttctta
     2101 gtttttatga tagtaatttg tttaagaaaa
//
LOCUS       DAOQAN010000174         2125 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_76826_length_2125_cov_2.329469, whole genome shotgun sequence.
ACCESSION   DAOQAN010000174 DAOQAN010000000
VERSION     DAOQAN010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76826_length_2125_cov_2.329469"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            166..930
                     /locus_tag="PKO10_03540"
     CDS             166..930
                     /locus_tag="PKO10_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66257.1"
                     /translation="MKPALVSGLNDIFEKIDIKIDLDKVSADKLILPLKESLGLNYKD
                     IANDDLKRFEISLKDGIYINDIKLLEEKDLKFTQKESDFEYYDDENLTTSYDMIGENL
                     LKITFGYKSSLNENSQKGLVVSFPQLKDKSRVVSTILGDLKEINVYEPNSELFTINPY
                     ESIKNSFLAIEAYDDTLSENSLKEFSIILNIKDFQAEILEINFRAYSIGSTEANGTIN
                     YEIVPKIGTSFTKDEQQYPVKISDIELSEVIEQKVE"
     gene            complement(954..1253)
                     /locus_tag="PKO10_03545"
     CDS             complement(954..1253)
                     /locus_tag="PKO10_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014474536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3817 domain-containing protein"
                     /protein_id="HNW66258.1"
                     /translation="MLRTKFSQFRAISIIEGISYLILVFLAMPLKYLFEEPMAVKIFG
                     MMHGIFFIFFCIALYNAMKHYKWGFLFSLKLFIYSLIPFLFILIEKEIMKTKKPI"
     gene            1341..>2125
                     /locus_tag="PKO10_03550"
     CDS             1341..>2125
                     /locus_tag="PKO10_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014474535.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HNW66259.1"
                     /translation="MNIENKNIILTTLNSRFSHSSLSLRYLFANLKELKDEAKILEFV
                     INSQNQTIVEEILEYKPKIVLISTYIWNAQDVAEIVKYIKLISPNTIVALGGPEISHL
                     PHRVDFEMADYFMFGEGEISVYNLCKNILGGIRPKDKIIHAKPVEFDKIALPYDYYTD
                     FDIKNRHIYIESSRGCPFTCEFCLSSIDSSMRYLPIDVFLNEIDKLWNRGARNYKFID
                     RTFNLKISYAKAILEYFLAKDEDYFLHFEVIPDNFPPELRELL"
BASE COUNT          823 a          232 c          324 g          746 t
ORIGIN      
        1 tgatactttg atattttttc aaaagctttt gatagttttt ttgaagagct tgtaaaaagt
       61 gatgtaaaag tttcatcttc tggagtttct tcttcatttt ctcaaaattc tgagaagatt
      121 tttgaaaaac taagatatga agcaaattta tctttaaata aaaatatgaa accagcatta
      181 gttagtggat taaatgatat ttttgaaaaa attgatataa aaattgattt agataaagtt
      241 tcagcagata agttaatctt acctttaaaa gagagtttag gattaaatta taaagatatt
      301 gcaaatgatg atttaaagag atttgagata tctttaaaag atggtatcta tataaatgat
      361 attaaattat tggaagaaaa agatttaaaa tttactcaaa aagagagtga ttttgaatat
      421 tatgatgatg aaaatcttac gacttcttat gatatgattg gtgaaaatct tctaaaaata
      481 acttttgggt ataaatcttc tttaaatgaa aatagtcaaa aaggattagt tgtatctttt
      541 cctcaattaa aagataaatc aagagttgtt tctactattt taggagattt aaaagagata
      601 aatgtttatg aaccaaatag tgagcttttt actataaatc cttatgaaag tattaaaaat
      661 agctttttag ctattgaagc ttatgatgat actttgagtg aaaattcact aaaagagttt
      721 agcataatct taaatatcaa agattttcaa gctgagattt tggagataaa ttttagagca
      781 tactcaattg gttcaactga ggcaaatgga actataaatt atgagatagt tccaaaaata
      841 ggaactagtt ttacaaaaga tgaacaacag tatcctgtaa aaatttctga tattgaatta
      901 tctgaagtaa ttgagcaaaa agtagagtaa aattatttag aaagctttgc tttctaaata
      961 ggcttttttg ttttcataat ctctttttct attagtataa ataaaaaagg gattaaagag
     1021 tatataaata gttttaaact aaataaaaat ccccatttgt agtgtttcat agcattgtat
     1081 aaagctatac aaaaaaatat aaaaaatatt ccatgcatca ttccaaatat tttaacagcc
     1141 ataggttctt caaatagata ttttagaggc attgctagaa aaactagtat taaataagag
     1201 attccttcaa ttatagatat agctctaaat tgagaaaact tcgttcgtaa catttcacac
     1261 cttttgtttt ttcttttaag tatagtaaac tattctttat gaaaggttaa tataacacca
     1321 ttttaagtag aatccacacc atgaatatag aaaataaaaa tataatatta acaacactaa
     1381 actcaagatt ttcgcactca agtctatctt tgagatactt gtttgcaaat ctaaaagagt
     1441 taaaagatga agcaaagatt ttagagtttg taataaatag ccaaaatcaa acaatagttg
     1501 aagagatttt agagtacaaa ccaaaaattg tattaatctc aacatatatt tggaatgctc
     1561 aagatgtagc cgaaattgta aaatatataa aactaataag cccaaataca atagtagctc
     1621 ttggaggtcc agagattagt catcttcctc atagggtaga ttttgaaatg gctgattact
     1681 ttatgtttgg agagggcgaa ataagtgttt ataacttatg taaaaatatc ttaggtggaa
     1741 ttagaccaaa agataagata attcatgcaa agccagttga gtttgataaa atcgctcttc
     1801 catatgatta ttacacagat tttgatataa aaaatagaca tatttatata gaaagtagcc
     1861 gtggttgccc ttttacttgt gaattttgtc tatctagtat tgacagttca atgagatatc
     1921 ttcctattga tgttttttta aatgagattg ataaactttg gaataggggg gcacgaaact
     1981 acaagtttat agatagaact tttaatctaa aaatatctta tgcaaaagca atattggagt
     2041 attttttagc aaaagatgag gactattttt tacactttga ggtaattcct gataattttc
     2101 cacctgagtt aagagagctt ttgaa
//
LOCUS       DAOQAN010000175         2124 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_76893_length_2124_cov_2.184147, whole genome shotgun sequence.
ACCESSION   DAOQAN010000175 DAOQAN010000000
VERSION     DAOQAN010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76893_length_2124_cov_2.184147"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..776
                     /locus_tag="PKO10_03555"
     CDS             <1..776
                     /locus_tag="PKO10_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipid A biosynthesis acyltransferase"
                     /protein_id="HNW66260.1"
                     /translation="KKHIKVARANLDFVYGNSISEDRKNEIIYNSYKSLVFNMYEFIE
                     NQNIEKEDLLKKANILNKDIIENAIKENRKIVYISAHYGGWELTVPYIALMFGDVAIV
                     NRRMNNPHIQKKYEKARSKNHITMLEKESAAKGMIKAFKENKTVCVVIDQYLNSGIDI
                     EFLGKITKATDSTSRIALKFDAVIIPIFTLCNDFRDWTIKVCEPIDVKTYEFKSEDKI
                     KELTQIQNDILSKQILEKPDFWLWQHKRFKNSNKEIYAK"
     gene            766..1356
                     /locus_tag="PKO10_03560"
     CDS             766..1356
                     /locus_tag="PKO10_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005870733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HNW66261.1"
                     /translation="MQNSSFLKPSLYIFAGINGAGKTTLYYKELENGTDLGRRINIDE
                     IVQAIGDWKNTSDQTRASKIALKHRTLCLENLYTFNIETTLSGKGILALVKKALQLDY
                     DVNLFYVGLENSELAKDRVAIRMLKGGHFVDNKTIDRRYPKSMKNLFKVLPLAKNVFI
                     YDNSNNFKLIAQKKENQELEVLEHVAWFDNLLKELS"
     gene            1353..>2124
                     /locus_tag="PKO10_03565"
     CDS             1353..>2124
                     /locus_tag="PKO10_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66262.1"
                     /translation="MNSFEYIAKKEYEKSNNDIFPFIFEEKQYWLKKARATKPDKIQK
                     FFYKFFPFELLIPPIVKSSKEALLYEISKLEKFKELGINTPVIAYKCEDFFVLEDSGN
                     SVNAYLRNKDISEDRFYFFVDKLIFELSKIHNFSQFHGGSQLRNFTFKDDKVFVLDFE
                     ESFDTNVDIKTLQYRDFLLFLLSFIKIKEVNFKIDYEKIINKYCELTNNIEFKQKLIR
                     FSKNCLLFRIFSAINTLTGFFPAVHRKRSDCSFCFFLNL"
BASE COUNT          857 a          202 c          333 g          732 t
ORIGIN      
        1 ataaaaaaca tataaaagta gcacgtgcaa acttagattt tgtgtatgga aatagtataa
       61 gtgaagatag aaaaaacgag attatttata actcttataa atctttggtt tttaatatgt
      121 atgaatttat tgaaaatcaa aatatagaaa aagaagattt actaaaaaaa gcaaatattt
      181 taaataaaga tataatagaa aatgcaataa aagagaatag aaaaattgtt tatatttcag
      241 ctcattatgg tggttgggag ctaactgttc catatattgc acttatgttt ggtgatgttg
      301 caatagtaaa tagaaggatg aataatcctc atattcaaaa aaaatatgaa aaagctagaa
      361 gtaaaaatca cattacaatg cttgaaaaag agagtgctgc aaagggtatg ataaaagctt
      421 ttaaagagaa taaaacagtt tgtgtagtta ttgatcaata cttaaatagt ggaatagata
      481 tagagttttt aggaaaaatt acaaaagcta cggattcaac ttcaagaatt gcgttaaaat
      541 ttgatgctgt tattattcca atttttactc tttgcaatga ttttagagat tggactatta
      601 aagtttgtga accaattgat gttaaaactt atgagtttaa aagtgaagat aaaatcaaag
      661 agttaactca aattcaaaat gatattttgt ctaagcaaat tttagagaaa ccagattttt
      721 ggctatggca acataagaga tttaaaaatt ctaataagga aatttatgca aaatagtagt
      781 tttttaaaac cttctttata tatttttgca ggtataaatg gtgcaggaaa aacaactttg
      841 tattataaag agctagaaaa tggtacagat ttgggaagaa gaatcaatat tgatgagatt
      901 gtccaagcta ttggagattg gaaaaataca agtgatcaaa caagagcttc aaaaattgct
      961 ttaaaacata gaactttgtg tttagaaaat ttatatactt ttaatataga aacaactttg
     1021 agtggaaaag gtattttagc tcttgttaaa aaagctttac aattggacta tgatgtaaat
     1081 cttttttatg ttggacttga aaatagtgaa ttagcaaaag atagggtagc tattcgtatg
     1141 ttaaaaggtg ggcattttgt tgataataaa actattgata gaagatatcc aaaatctatg
     1201 aaaaatcttt ttaaagtttt acctttggct aaaaatgttt ttatatacga taatagtaat
     1261 aattttaaac taatagcaca aaaaaaagag aatcaagaat tagaagtttt agaacacgta
     1321 gcgtggtttg ataatttact aaaagagtta tcttgaatag ttttgaatat atagcaaaaa
     1381 aagagtacga aaaatcaaat aatgatattt tcccttttat ttttgaagaa aaacagtatt
     1441 ggctaaaaaa agctagagct acaaaacctg ataaaataca aaagtttttt tataagttct
     1501 ttccttttga actacttatt ccacctattg taaaaagttc aaaagaggct ttgctttatg
     1561 aaatatcaaa gcttgaaaaa tttaaagagt taggaataaa tactcctgtt atagcttata
     1621 aatgtgaaga tttttttgtt ttagaagata gtggaaatag tgtaaatgct tatttaagaa
     1681 ataaagatat aagcgaagat agattttact tttttgtaga taaattgata tttgaactat
     1741 ctaaaattca taatttttca caatttcatg gtggaagtca actaagaaat tttactttca
     1801 aagatgacaa agtttttgtt ttagattttg aagagagttt tgatactaat gtagatataa
     1861 aaactttaca atatagagat tttttgttat ttttgttatc atttataaaa ataaaagaag
     1921 ttaattttaa aatagattat gaaaagataa ttaataaata ttgcgaactt acaaacaata
     1981 tagaatttaa acaaaaatta ataagattta gcaaaaattg ccttcttttt agaatctttt
     2041 ccgcaataaa tacacttacc ggtttcttcc ctgccgtcca taggaagaga tctgattgta
     2101 gcttttgttt cttcctgaat cttt
//
LOCUS       DAOQAN010000176         2122 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_76991_length_2122_cov_2.539429, whole genome shotgun sequence.
ACCESSION   DAOQAN010000176 DAOQAN010000000
VERSION     DAOQAN010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76991_length_2122_cov_2.539429"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..593
                     /locus_tag="PKO10_03570"
     CDS             <1..593
                     /locus_tag="PKO10_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66263.1"
                     /translation="AMALDVEDIDDALYYSSKIEKSKDKEILKEEFLYKYQILKIDNN
                     TAKLDKFFKNSLENNDLIEANIEKPIIIDFYYDLYLYLIKEGKEEEAFKILNSLNNKQ
                     NEFKAFVYSPFVESELSRLLKKQNNFQDAVNYLVLALEHAKNIKPEDEVKLYYDILTL
                     YDNLGQKEQKEIYLQKCKNINIEDNFYKNMCNGMNP"
     gene            590..1894
                     /gene="fliI"
                     /locus_tag="PKO10_03575"
     CDS             590..1894
                     /gene="fliI"
                     /locus_tag="PKO10_03575"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014475077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HNW66264.1"
                     /translation="MNIDEIIDNIDSNNLNIAFGRVVNISAVTLTAVGLDVAVGDIVR
                     IESVQKLYTVLGMVTVLNDDMFVIVPFSFIDGFKINDKVFLQRDGLTIKCGNGLLGRV
                     VNALGEPIDDLGKIRDLDTNAPINKESMSPLDRGIIDQKFATGVKAIDSMLTCGKGQK
                     VGIFAGSGVGKSTLMGMIVQGCEATIKVIALVGERGREIPEFIHYNLNNNLENTVIVT
                     ATSDESPLMRKYAAFTAMSIAEYFRDKGHDVLLMMDSVTRFAMAQREIGLSTGEPPVS
                     RGYPPSVFALLPQLMERAGNSKKGSITAFFTVLVDGDDLNDPIADQSRSILDGHIVLT
                     RDLTEQGFYPPINILKSPSRVIDKVVTKEHYNDFLKLKRVLSLIKENEVLIRVGAYKK
                     GMDLELDSAIAKKEKAREFLTQGTLEKYSFDEIVANLRKVLI"
     gene            1891..>2122
                     /locus_tag="PKO10_03580"
     CDS             1891..>2122
                     /locus_tag="PKO10_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein 3"
                     /protein_id="HNW66265.1"
                     /translation="MISLNSTTYRLGNLDNYQAKLNFQMGGSKLQFGSDDSVTFGRLT
                     HTEDKIKTQKGIVAQIERADVLNKTSDTAMKEV"
BASE COUNT          833 a          216 c          401 g          672 t
ORIGIN      
        1 aagctatggc tcttgatgtt gaagatatag atgatgcttt gtattactca tcaaaaatag
       61 aaaaatctaa agataaagag attttaaaag aggagttttt gtataaatat cagattttaa
      121 aaattgataa taatacagca aaattagata aattttttaa aaatagtttg gaaaataatg
      181 atttaattga ggcaaatata gaaaaaccaa taattattga tttttattat gatttatatc
      241 tatatttaat aaaagaggga aaagaggaag aggcatttaa gattttaaat tctttaaata
      301 ataagcaaaa tgagtttaaa gcttttgttt attctccttt tgtagagagt gaattatcaa
      361 gattattaaa aaaacaaaat aatttccaag atgcagtaaa ctatctagta cttgccttag
      421 agcatgctaa aaatataaaa ccagaggatg aggtaaaact ttactacgat attttaactt
      481 tgtatgataa tttgggacaa aaagaacaaa aagagatata tttgcaaaag tgtaaaaata
      541 taaatataga agataatttt tataaaaata tgtgtaatgg aatgaatcca tgaatataga
      601 cgaaattata gataacatag attcaaataa tttaaatata gcctttggaa gagttgtaaa
      661 tatctctgct gttactttaa ctgctgttgg tttagatgtt gctgttggag atattgtaag
      721 aattgaatca gtacaaaagc tatatacagt tttaggaatg gttacagttt taaatgatga
      781 tatgtttgtc attgttccat tttcatttat tgatggtttt aagataaatg ataaagtatt
      841 tttacaaaga gatggactta ctataaagtg cggaaatggt cttttaggaa gagttgtaaa
      901 tgctttggga gaacctattg atgatttggg aaaaataaga gatttagata caaatgcacc
      961 tataaataaa gagagtatgt cgccgcttga tagagggatt attgatcaga aatttgcaac
     1021 gggtgtaaag gctattgact caatgcttac atgtggtaaa ggtcaaaaag taggtatttt
     1081 tgcaggaagt ggagttggaa aatcaactct tatgggaatg atagttcaag gatgtgaagc
     1141 aactattaaa gtaattgcac ttgttgggga aagaggaaga gagattcctg aatttattca
     1201 ctataattta aataacaatt tagaaaatac tgtaattgta acagcaactt cagatgagtc
     1261 accacttatg cgaaaatatg ctgcttttac agctatgagc atagctgaat attttagaga
     1321 taaagggcat gatgtacttc ttatgatgga tagtgttact agatttgcta tggctcaaag
     1381 agagataggt ctaagtacag gagaaccacc agttagtagg ggttatccac catcagtgtt
     1441 tgcactttta ccacaactta tggaaagagc aggtaatagt aaaaaaggct ctattacagc
     1501 attttttaca gttttagttg atggagatga tttaaatgat ccaattgcag atcaaagtag
     1561 gtctattctt gatggacata tagtactaac aagagattta acagaacaag gtttttatcc
     1621 accaataaat atattaaaat caccttcaag ggtaatagat aaagttgtta caaaagagca
     1681 ttataatgat tttttaaagt taaaaagagt attatcacta ataaaagaga acgaagtttt
     1741 aattagagtt ggtgcataca aaaaaggtat ggatcttgag ttagatagtg caatagcgaa
     1801 aaaagagaaa gcaagagagt ttttaacaca aggaactctc gaaaagtact cttttgatga
     1861 aattgttgca aatttaagaa aggtgttaat atgatatcat taaattctac aacatataga
     1921 ttaggaaatt tagataatta tcaagcaaaa cttaattttc aaatgggtgg aagtaaactt
     1981 caatttggaa gcgacgattc tgttactttt ggaagattaa ctcatacaga agataaaata
     2041 aaaactcaaa agggaattgt agcgcaaata gagagagctg atgttttaaa caagacatca
     2101 gatactgcaa tgaaagaggt aa
//
LOCUS       DAOQAN010000177         2121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_77031_length_2121_cov_2.740561, whole genome shotgun sequence.
ACCESSION   DAOQAN010000177 DAOQAN010000000
VERSION     DAOQAN010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77031_length_2121_cov_2.740561"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /gene="soxX"
                     /locus_tag="PKO10_03585"
     CDS             <1..287
                     /gene="soxX"
                     /locus_tag="PKO10_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014473402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxX"
                     /protein_id="HNW66266.1"
                     /translation="NSELIKKGEEIYTTNTKGNCIACHDANGKTLDGPGTMGPKLQYL
                     AMWPDEVLYNKIFDPTNPGDPITAMPAFGRNGWLSDEEIKAVVAYLKTIN"
     gene            303..755
                     /gene="soxY"
                     /locus_tag="PKO10_03590"
     CDS             303..755
                     /gene="soxY"
                     /locus_tag="PKO10_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier protein SoxY"
                     /protein_id="HNW66267.1"
                     /translation="MLNRRKFLGLGLGAIAVAAIPSSLSAEDFRASKPKAWTATKVDD
                     AVKEIFGTTTTTQGGITLTAPDIAENGAVIPISFKTDLKASKIAVFQDANPESAVAVF
                     TTTPDMVLDYAFRIKMAKTGTVTVIADVGGKLHSVSKEIKVTIRGCGG"
     gene            830..1138
                     /gene="soxZ"
                     /locus_tag="PKO10_03595"
     CDS             830..1138
                     /gene="soxZ"
                     /locus_tag="PKO10_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014473404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier complex protein
                     SoxZ"
                     /protein_id="HNW66268.1"
                     /translation="MAGTTKIKAKIDKNGVCEVKALASHDMLSYQEAERAKKEANFIT
                     YLVAKVGGKVVYEVSSSQFLSKDPYFKFSFTGAKAGDDIEITWKDLKGNSDTTVEKIK
                     "
     gene            1174..2088
                     /gene="soxA"
                     /locus_tag="PKO10_03600"
     CDS             1174..2088
                     /gene="soxA"
                     /locus_tag="PKO10_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016669 - oxidoreductase activity, acting
                     on a sulfur group of donors, cytochrome as acceptor
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019417 - sulfur oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxA"
                     /protein_id="HNW66269.1"
                     /translation="MLIKIVKSTTVLALFISSLSAASFNSGAEKDRLEMIKFFEAKFE
                     DPAKNKDRFFTYFTEEELEQKYDKNLKHMDFNIGSYAYSKDARSQYEALKEMPPYEDA
                     IEKGEELYTKKFANGNSLQTCFPDLTNAGTYPYYDKNKKELISLTKAVNDCLRANGEK
                     EWGTKKGPMAEFQAYWVNESKEAGKKFDIKINSKAEKEAYERGKEYYYSQRGYLKLSC
                     ATCHIQGSGQRVRNEVLSPLLGQVTHFPVLRLKWTDIGTVERRLSGCVVDQGQVPPKD
                     ESQTMIELIYFLAYMSNDMPVDGPDVRK"
BASE COUNT          862 a          246 c          399 g          614 t
ORIGIN      
        1 ataattcaga attgattaaa aaaggtgaag agatatatac tactaatact aaaggtaatt
       61 gtatagcttg ccatgatgca aatggaaaaa cgctagatgg tcctggaaca atgggaccaa
      121 aattgcaata tcttgcaatg tggccggatg aagttttata taataaaatt tttgacccaa
      181 caaatccagg tgatccaata actgcaatgc cagcttttgg aagaaatgga tggttaagtg
      241 atgaagaaat aaaagcagtt gttgcttatt taaaaacaat aaattaaaat aaaggaagaa
      301 ttatgttaaa tagaagaaaa tttttaggtt taggtttagg tgctattgca gttgctgcaa
      361 ttccaagtag tttaagtgca gaggatttta gagctagcaa accaaaagct tggacagcta
      421 caaaagttga tgatgcagtt aaagaaattt ttggaacaac tactacaact caaggtggta
      481 ttacattaac agctccagat attgctgaaa atggtgcagt tattccaatc tcttttaaaa
      541 cagatttaaa agcaagtaaa attgcagtat tccaagatgc aaatccagaa agtgcagttg
      601 ctgtatttac aacaactcct gatatggttt tagattatgc atttagaatt aaaatggcaa
      661 aaacaggtac tgttacagta attgcagatg ttggtggaaa attacactca gtttctaaag
      721 agataaaagt aacaattaga ggatgtggtg gttgatttag ttcagataat cattaaataa
      781 aagataaaaa atagaataaa aaattataaa ataaaaaaaa ggatataaaa tggcaggaac
      841 aacaaaaata aaagcaaaaa ttgataaaaa tggtgtatgt gaagtaaaag cattagcaag
      901 tcatgatatg ttaagttacc aagaggctga aagagctaaa aaagaggcaa actttattac
      961 atatttggtt gcaaaagttg gtgggaaagt tgtatatgaa gtatcttcta gtcaattctt
     1021 atcaaaagac ccttacttca aattttcatt tacaggtgca aaagctggag atgatattga
     1081 aattacttgg aaagatttaa aaggaaatag tgatacaaca gtagagaaaa tcaaatagtt
     1141 ttaactattt gattttaaat aaaaggagag aaaatgctta taaaaatagt taaatctact
     1201 actgttttag ctctatttat atcatcttta agtgctgcaa gctttaattc tggtgcagaa
     1261 aaagatagat tagagatgat aaagttcttt gaagcaaaat ttgaagaccc agcaaaaaat
     1321 aaagatagat tttttacata tttcacagaa gaagaacttg aacaaaaata tgataaaaat
     1381 ctaaaacata tggattttaa tattggaagc tatgcatatt caaaagatgc tagaagccaa
     1441 tatgaagcac taaaagagat gccaccttat gaagatgcta ttgaaaaagg tgaagagtta
     1501 tatactaaaa aatttgcaaa tggtaactca ttgcagacat gtttccctga tttaacaaat
     1561 gctggaacat acccttacta tgataaaaat aaaaaagagt taatctcttt aacaaaagct
     1621 gttaatgatt gtttaagagc aaatggcgaa aaagagtggg gaacaaaaaa gggtcctatg
     1681 gctgaatttc aggcttattg ggtaaatgaa agtaaagaag ctggtaaaaa gtttgatata
     1741 aaaatcaata gtaaagctga aaaagaggct tatgaaagag gaaaagagta ctactattct
     1801 caaagaggat atttaaaact atcttgtgct acttgtcata ttcaaggttc tggacaaaga
     1861 gttagaaatg aggttttatc accactttta ggtcaagtta cacactttcc agttctaaga
     1921 cttaaatgga cagatatagg aacagttgaa agaagacttt ctggttgtgt tgtagatcaa
     1981 ggtcaagttc caccaaaaga tgagagccaa actatgattg agttgatata tttcttagct
     2041 tatatgtcaa atgatatgcc agttgatggt ccagatgtaa gaaaataaag gatattaaaa
     2101 tgaaaaatat tttaaaaatt t
//
LOCUS       DAOQAN010000178         2121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_77040_length_2121_cov_2.305421, whole genome shotgun sequence.
ACCESSION   DAOQAN010000178 DAOQAN010000000
VERSION     DAOQAN010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77040_length_2121_cov_2.305421"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="PKO10_03605"
     CDS             <1..276
                     /locus_tag="PKO10_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66270.1"
                     /translation="VSYDIKVHSSGKDKPYFSDQKYFRNADSFVNAKGFVDRILKWKS
                     ANKNYSKKFNEVKAQSRTSYEAFEYFFEEELKTAVKEKLTNKLRVCK"
     gene            complement(391..1983)
                     /locus_tag="PKO10_03610"
     CDS             complement(391..1983)
                     /locus_tag="PKO10_03610"
                     /inference="COORDINATES: protein motif:HMM:NF033559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HNW66271.1"
                     /translation="MFVRKKKNSSGSVSIQIISKNNNKYKVVETIGCGRNEFEVEQLL
                     QNAKNRLIELEPNLFDFMNYNNQKLTNKDMRVIGDELIFGKLFKDLGCENILFKKEED
                     KEIFKALVVSRLLYPGSKLYLIDYYHIYKKRKIDKNKIYRCLDNIYEDHLKQNIEKCI
                     FNHTNEKMNNTITFTFYDVTTLYFESESEDDLRRVGFSKEGKLARPQIQLGLFTTLQG
                     YPLSYEVYEGNKFEGHTLIDILKKFQEKFNLSSKPVVVADRGMLNNDNLAYLETNGYK
                     YIIAAKIKNISNELKEEISNLSFIDDSTIHTININKELIYKEQNGEIDLESRKIVAKK
                     SLNIKQRLILSYSSKRAKKDKYTREKAIEKIQSKISKNITKSDLKLSYYAKYLDIDNT
                     LDIKYRLNPKKIIEDEKLDGIKGFITNDFTLNPNEIIQHYQNQYDIEKAFRISKTDLK
                     IRPIYHRLETRIKAHILISFVAYAVYKEFERKIKLSNINVKFKLTFDYIKTMYGYKTL
                     FGVEPLEMDEIQRQIYDAIYSK"
BASE COUNT          713 a          295 c          220 g          893 t
ORIGIN      
        1 gtctcttatg atattaaagt tcatagctct ggtaaagata aaccttattt ttcagaccaa
       61 aagtatttta gaaacgctga ttcatttgta aatgctaagg gttttgtaga tcgtattctc
      121 aagtggaaga gcgctaataa aaattactct aaaaaattta atgaagtaaa agctcaatca
      181 agaactagtt atgaggcttt tgaatatttt tttgaagagg aattaaaaac agccgttaaa
      241 gaaaagctca ctaataaact tagagtttgc aaatgaatat aaaaatattt taaacttatt
      301 tgattcaaaa aataaaaaat atatctaaaa ataaatagca agaaaatctt gctatttatc
      361 tcctgagttt ctcataggac acccaaattt tcattttgag taaatagcat cataaatttg
      421 cctttgaatt tcatccattt caagtggttc aacaccaaac agtgttttat atccatacat
      481 tgttttaata taatcaaatg taagtttaaa ttttacatta atatttgata attttatttt
      541 tctttcaaac tctttataaa ctgcatatgc tacaaatgaa attaaaatat gagcttttat
      601 tcttgtttct agtctatgat aaattggtct tatttttaaa tctgttttag aaattctgaa
      661 tgctttttca atatcatatt gattttgata atgttgtatt atttcattcg gatttaatgt
      721 aaaatcattt gtaataaatc cttttatacc atcaagtttt tcatcttcta taattttctt
      781 aggattaagt ctatatttta tatcaagcgt attatctata tccagatatt ttgcgtaata
      841 tgataatttt aaatcacttt tagtaatatt ttttgaaatc ttactttgaa ttttctcaat
      901 tgctttttct ctggtatatt tatctttttt tgctcttttt gatgagtagc ttaatatcaa
      961 tctttgttta atattaaggc ttttcttagc tactattttt ctgctttcta aatcaatctc
     1021 tccattttgt tctttataaa ttaattcttt atttatattt atagtatgaa ttgtagaatc
     1081 atcaataaag cttagatttg aaatctcttc ttttagttca tttgaaatat tttttatttt
     1141 tgctgcgatg atatacttat agccattagt ctctaaataa gctaaattat cattatttaa
     1201 cattcctcta tctgcaacca ctactggttt tgaagataaa ttaaacttct cttgaaactt
     1261 ttttaaaata tctattaatg tatgcccttc aaatttatta ccttcataaa cctcataaga
     1321 tagtggataa ccttggagtg ttgtgaataa acctaattgt atttgaggtc tagctaattt
     1381 accctctttt gaaaaaccta cccttcttaa gtcatcttca ctctcacttt caaagtaaag
     1441 tgttgttaca tcataaaatg taaatgttat agtattattc attttttcat ttgtatgatt
     1501 aaatatgcat ttctcaatat tttgttttag atgatcttca taaatattat ctaggcatct
     1561 atatatttta tttttatcaa tttttctttt tttataaata tgataataat ctatcaagta
     1621 taatttactt ccaggatata gtaatcttga gactactaat gctttaaata tctctttatc
     1681 ttcttctttt ttgaataata tattctcaca accaagatct ttaaaaagct ttccaaatat
     1741 tagctcatca ccaattactc tcatatcttt atttgttaat ttctgattat tataattcat
     1801 gaaatcaaac aaatttggtt ctaattcaat taatctattt ttagcattct gaagtaattg
     1861 ttcaacttca aattcatttc ttccacaacc aatagtttca actactttat atttattatt
     1921 attttttgaa attatctgaa tactaacaga tccactacta ttttttttct tacgaacaaa
     1981 catgatgaaa ttttatcaat ttaagctttg ggacaccata atttaaagat tttaacttct
     2041 gtaaatacca tcaacaaggg attcatgatt ttatcttgaa aaaattgaga aactcaggaa
     2101 aaaagtacct taaaaaagat g
//
LOCUS       DAOQAN010000179         2097 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_78436_length_2097_cov_2.341822, whole genome shotgun sequence.
ACCESSION   DAOQAN010000179 DAOQAN010000000
VERSION     DAOQAN010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78436_length_2097_cov_2.341822"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="PKO10_03615"
     CDS             <1..429
                     /locus_tag="PKO10_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66272.1"
                     /translation="NLKNRKRRFMLNKKIIISYLTFCLLSSSIYAKQSDDERESKKRD
                     YKHSNYHKDKDYSDKKIKSEKQKDIPPGLQKKVDNGGTLPPGWQKKIEKGEVVNSTIL
                     SNGRIVTSKYPKIKNTEVYEVENRVFRIYKDTKEILDILK"
     gene            complement(472..1896)
                     /locus_tag="PKO10_03620"
     CDS             complement(472..1896)
                     /locus_tag="PKO10_03620"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014473545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HNW66273.1"
                     /translation="MKINILEISKNKDFDLEIVLVNNLNQIEYEKDREILENLEFKVK
                     DETAVLLAQSKKIYASFEEFTYDSLAIAMATAIKRFNSTKYKSVKLLLNNSLKDNFKA
                     LVEGSILGSYSFDNYKSEKSNKKQELAFVVDDKDSQLTTILKESQTICEAVNSARDMV
                     NTAPADFTPKSFVKEAQNIAKEFELDCEILGEKDLEKQKMMSMHSVGRASIHESQLIH
                     LKYKPKKAIKKVVLVGKGLTYDSGGLSLKPADFMITMKADKSGGVAVLNTIKVIAKLK
                     LPIEVHAIIGAVENMIGGNAYKPDDILRAKNGKTIEVRNTDAEGRLVLADCLCYAQDE
                     IKNIDYLLDFATLTGACVVGLGEYTTGIMGNSEELKHQALVSAQNSGEYATKLDFNRY
                     LKKCIKSEIADVCNISNTRYGGAITAGMFLDNFIYEENKNRWIHFDIAGPAFVEKAWG
                     YNPYGASGTGVRMAVQFIKDLTKL"
     gene            complement(1886..>2097)
                     /locus_tag="PKO10_03625"
     CDS             complement(1886..>2097)
                     /locus_tag="PKO10_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014473544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HNW66274.1"
                     /translation="FINLISAWCWAAITIVPVWYFGNQIMVVLHWAKEHWYLAIPIAA
                     IFGGGIIYYFNKATKKIEKRVMDEN"
BASE COUNT          751 a          333 c          232 g          781 t
ORIGIN      
        1 aatttaaaaa atagaaaaag gagatttatg ttaaataaaa agattattat ttcatattta
       61 acattttgtt tattaagttc atctatttat gcaaaacaaa gtgatgatga aagagaaagt
      121 aaaaaacgag actataagca tagtaattat cataaagata aagattatag tgataaaaaa
      181 ataaaatctg aaaaacaaaa agatattcct cctggtttac aaaaaaaagt agataatggt
      241 ggaactcttc ctcctggatg gcagaaaaaa atagaaaaag gagaagttgt aaatagtact
      301 attttgagta atggaagaat tgttacaagt aaatatccga aaataaaaaa tacggaagtt
      361 tatgaagttg aaaatagagt ttttagaatt tataaagata caaaagagat tttagatata
      421 ttaaagtagt aaaagttggt ataaaatacc aacttttatt taagtattaa attataattt
      481 tgttaagtct tttataaatt gaacagccat tctaacacct gttccacttg caccgtatgg
      541 attatatccc caagcttttt ctacaaatgc aggtccagct atatcgaaat gtatccatct
      601 gtttttattc tcttcatata taaaattatc taaaaacatt ccagcagtta tagctccacc
      661 atatctagta tttgatatgt tacaaacatc tgctatttct gattttatac atttttttaa
      721 atatctatta aaatcaagtt tagtagcata ctctccagaa ttttgtgctg atactaaagc
      781 ttgatgttta agttcttcac tatttcccat aattcctgtt gtatattcac caagtccaac
      841 aacacaagca ccagttaaag ttgcaaaatc taaaagatag tctatatttt taatctcatc
      901 ttgagcataa cataaacaat ctgcaagaac taatcttcct tctgcatctg tatttcttac
      961 ttctatagtt ttaccatttt tagctcttaa aatatcatct ggcttataag catttccacc
     1021 aatcatattt tcaactgctc caattattgc atgaacttca ataggtaatt ttaattttgc
     1081 aataactttt atagtattta aaacagcaac tccacctgat ttatcagctt tcatagtaat
     1141 cataaaatca gctggtttta aagataatcc accactatca taagttaaac cttttccaac
     1201 aagtacaact tttttaatag cttttttagg tttatattta agatgtatta gttgtgattc
     1261 atgaatagaa gcacgtccca cactatgcat tgacatcatt ttttgctttt ctaaatcttt
     1321 ttcacctaaa atttcgcaat caagttcaaa ctcttttgca atattttgtg cctcttttac
     1381 aaaacttttt ggtgtaaaat cagcaggagc agtgtttacc atatctcttg cactatttac
     1441 tgcctcacaa atagtttgag actcttttaa aatagtagtt aattgagaat ctttatcatc
     1501 aacaacaaaa gctaactcct gttttttatt acttttttca cttttataat tatcaaatga
     1561 atatgaacct aaaattgatc cttcaactaa agctttaaaa ttatctttta aactattatt
     1621 taataaaagt tttacactct tatattttgt tgagttaaat cttttaattg cagttgccat
     1681 tgcaattgct aaactatcgt atgtaaactc ttcaaaagaa gcatatattt ttttactttg
     1741 tgccaataaa actgctgttt catcttttac tttaaactca agattttcta aaatctctct
     1801 atccttttca tactcaattt gatttaaatt atttactaaa actatctcta aatcaaaatc
     1861 tttatttttt gatatttcta aaatattaat tttcatccat tacccttttt tcaatttttt
     1921 ttgttgcttt attaaaataa tatataattc ctccaccaaa aatagctgct attggaattg
     1981 ctaaatacca gtgctctttt gcccagtgta aaacaaccat gatctgattt ccaaaatacc
     2041 aaacaggaac gattgtaata gcagcccaac accaagcact tattaaattt ataaatg
//
LOCUS       DAOQAN010000180         2094 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_78588_length_2094_cov_3.005395, whole genome shotgun sequence.
ACCESSION   DAOQAN010000180 DAOQAN010000000
VERSION     DAOQAN010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78588_length_2094_cov_3.005395"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..867)
                     /locus_tag="PKO10_03630"
     CDS             complement(22..867)
                     /locus_tag="PKO10_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014467993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HNW66275.1"
                     /translation="MIFEHEIPKGSRLYFGKTAKAKRVLENSVCEILEKNGFEEILTP
                     NFSYSQHQSIEDNKKLIKFSDEENEQVSLRADSTLDVVRIITKRLGRATNHRKWFYIQ
                     PIFSYPSKEDYQIGCEWIEHNNISDIMNLTADILRAIKIEPILQISNINIPKLISTEL
                     NISIDILKNGDISELLKLDCDWLNNLLRVKDIKSLENIIEKVPNILKKELEILLEKSK
                     EVKYSNIIIAPMYYGSLKYYNGVYYRVIDKNLVLCRGGMYETDGISSLGFALYTDNLL
                     KMLEG"
     gene            complement(873..1979)
                     /locus_tag="PKO10_03635"
     CDS             complement(873..1979)
                     /locus_tag="PKO10_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HNW66276.1"
                     /translation="MLLTPGPTPVPEFARKAMSDITIHHRTKEFEAIFERTRNLLIEI
                     YNMPEVLMLASSGTGAMEACVTNFTRKKALTVNSGKFGERFGKICKAFNIDYTEIKNE
                     WNTAVSVEDIVNTIKNDSNIDAIFIQICESAGGLRHPVEEIAKEVKKINPEIIVVADG
                     ITALGVEKIDVTNIDALITGSQKAFMLPPGLAMIGLSQKAVEKIEEKSAGYYFNLATE
                     LKNQRKNTTAWTAATTLIMGLEAILVELKKVGFDNLYSKTALRAKATQEALKAIGFEI
                     YPKTPANAMTTVYTEQSNEIRKLLKTKYNVDIAGGQDHLAGKIFRINHMGLVEDFEAS
                     WAVNAIELVMDDLNIRKFDGTANRVFAQNMFKGI"
BASE COUNT          659 a          356 c          212 g          867 t
ORIGIN      
        1 actattacat ctgctttcat tttatccctc taacattttt aataaattat ctgtatataa
       61 tgcaaaacct agagagctga ttccatcagt ctcgtacatt ccacctctac ataaaactaa
      121 atttttatct attactctat aataaacccc attataatat tttaaacttc cataatacat
      181 aggagcaata attatatttg agtattttac ctcttttgac ttttccaaaa gaatttcaag
      241 ctcttttttt aaaatatttg gcactttttc tataatattt tctaagcttt ttatatcttt
      301 cactcttaaa agattattta accaatcaca gtctaacttt aaaagttcag atatgtcacc
      361 atttttaagt atatcaatac taatatttaa ctctgtactt attaattttg gtatatttat
      421 atttgatatt tgtaaaattg gttctatttt tatagctctt aatatatctg ctgttaaatt
      481 cataatatca gatatattat tgtgctctat ccattcacaa ccaatttgat aatcctcttt
      541 tgatggataa gaaaaaattg gttggatata gaaccatttt ctatgatttg tagctcttcc
      601 caatcttttt gtaataattc ttacaacatc taatgttgaa tctgctctta atgaaacttg
      661 ttcattttct tcatcagaaa actttattaa ttttttatta tcttcaatag attgatgttg
      721 agaatatgaa aaatttggag ttagaatctc ttcaaatcca tttttctcca aaatttcaca
      781 aacactattt tctaaaactc tttttgcttt tgctgtttta ccaaaataaa gtctgctacc
      841 ttttggaatt tcgtgttcaa atatcattaa aactatatcc ctttaaacat attttgggca
      901 aatactctat ttgcagttcc atcaaatttt ctaatattta aatcatccat aaccaattca
      961 atagcattta cagcccaaga agcttcaaaa tcttctacta atcccatatg attaattcta
     1021 aaaattttcc cagctaaatg atcttgtcca cccgcaatat caacattata ttttgttttt
     1081 aaaagctttc taatctcatt tgattgttct gtataaaccg ttgtcatagc atttgctgga
     1141 gtttttggat atatttcgaa tcctattgct tttaatgctt cttgagttgc ttttgctctt
     1201 aaagctgttt tactatataa attatcaaat cctacttttt ttaattcaac taaaatagct
     1261 tctaatccca taattaatgt agttgctgct gtccaagccg ttgtattttt tctttgattt
     1321 tttaactcag ttgcaagatt gaaataatat ccagctgatt tttcttctat tttttcaact
     1381 gctttttgtg ataatccaat cattgcaaga cctggaggaa gcataaatgc tttttgactt
     1441 ccagtaataa gtgcatcaat attagtaaca tctatttttt caacacctaa agctgtaatt
     1501 ccatccgcta caactattat ttctggatta atttttttaa cttcttttgc tatttcttca
     1561 acaggatgtc ttaatcctcc agcactttca catatttgaa taaatatagc atcgatattt
     1621 gaatcatttt tgattgtatt tacaatatct tcaacgctaa cagcagtatt ccactcattt
     1681 tttatttcag tataatctat attaaacgct ttacagattt ttccaaatct ctctccaaat
     1741 tttccagaat taactgtaag tgcttttttt ctagtaaaat tagttacaca agcctccata
     1801 gctcctgttc cacttgaagc tagcattaaa acttctggca tattataaat ttctattaaa
     1861 agatttctag ttctctcaaa aattgcttca aactcttttg ttctatgatg tattgtaata
     1921 tcgctcatag cttttcttgc aaattctggt acaggagttg gtcctggtgt taataacata
     1981 ttaattatcc ttattataaa tatttcatat ttcagtaaaa cggaatattt tagctaaaaa
     2041 aagattatta aatggttaaa aatataagtt atcaaaataa tgatatttta aatt
//
LOCUS       DAOQAN010000181         2089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_78859_length_2089_cov_3.597837, whole genome shotgun sequence.
ACCESSION   DAOQAN010000181 DAOQAN010000000
VERSION     DAOQAN010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78859_length_2089_cov_3.597837"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..845
                     /locus_tag="PKO10_03640"
     CDS             <1..845
                     /locus_tag="PKO10_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CofH family radical SAM protein"
                     /protein_id="HNW66277.1"
                     /translation="NPDQYTLSHEEILETVKNSSKNGIKEVHIVSAHNPHTGLEWYMD
                     IFKKIKKDFPNIHIKALTAAEIHFLSTQYNLSYEELIDTMIKSGVDSMPGGGAEIFDE
                     KVRKRICGGKVSSEQWLEIHKLWHSKGKKSNATMLFGHIETREHRVDHILRLRDLQDI
                     TGGFNAFIPLVFQTENNYLKVKEPVTANEILKTYAVSRILLDNIPNIKAYWATSTVKL
                     ALIAQEFGANDVDGTIEKESIQSAAGAKSKHGVAQNEFVDLIKNSGFIPVERDSVYNE
                     LKIY"
     gene            complement(878..1120)
                     /locus_tag="PKO10_03645"
     CDS             complement(878..1120)
                     /locus_tag="PKO10_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66278.1"
                     /translation="MYKILIQDKCKCFLNSNLNGSFEFSSKDDALFKAIEIKNIMNKT
                     FCKKHNFQIQEMFNNLVIKFYKDEPLSSCCGNGCCM"
     gene            1228..2031
                     /locus_tag="PKO10_03650"
     CDS             1228..2031
                     /locus_tag="PKO10_03650"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013136688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HNW66279.1"
                     /translation="MHITNLLSQYFGKFAKKEFPRPIQELINGAYTKFMKLDLKEFKN
                     SKHYKSLNELFTRDLIIKREIDSSKDIFISPTDSLITECGKLKNDTALQIKGMEYSVE
                     ELLTYYCRDNFLKLENGEFMNFYLSPRDYHRYHAPVDFKLKKLIHVPGKLYPVNLKYL
                     NKELELFVQNERVVLECEKDGKIFYMVFVGALNVGQMVFEFEPRVETNKDTKEIKVYT
                     YENIEISKADCLGYFKMGSTVVMIWEKDFVVLENLLNQNVKFGQKIAKY"
BASE COUNT          828 a          228 c          325 g          708 t
ORIGIN      
        1 aaaacccaga ccaatatact ttaagtcatg aagagatttt agaaactgta aaaaactcat
       61 ctaaaaatgg aataaaagag gttcatatag tttctgctca caatccacat actggactag
      121 agtggtatat ggatatattt aaaaaaatta aaaaagattt tccaaatata catataaaag
      181 ctcttacagc tgctgaaatt cattttttaa gtacacaata taatttgagc tatgaagaac
      241 ttattgatac tatgattaaa agtggtgttg attcaatgcc aggtggtgga gctgagattt
      301 ttgatgaaaa ggtgcgaaaa agaatctgcg gtggaaaagt aagctcagag caatggttag
      361 aaattcataa actttggcac agtaagggta aaaaaagtaa tgcaactatg ctttttggac
      421 atattgaaac aagagaacat agagttgatc atattttaag attaagagat cttcaagata
      481 ttacaggtgg atttaatgca tttatccctt tagtttttca aacagaaaat aactatttaa
      541 aagtaaaaga gccagtaact gcaaatgaaa ttttaaaaac ttacgcagtt tcacgaattt
      601 tacttgataa tattccaaat attaaggcat attgggcaac ttcaactgta aaattagctt
      661 tgattgctca agaatttggt gcaaatgatg ttgatggaac aatagaaaaa gagtcaatac
      721 aaagtgcagc aggggcaaaa agcaaacatg gtgttgctca aaatgagttt gttgatttaa
      781 taaaaaattc tggatttatt ccagttgaaa gagatagtgt ttataatgaa ctgaaaattt
      841 attaaaagtt atttaataaa ttttagttct ttatatatta catacaacaa ccatttccac
      901 agcaagagct aagaggttca tctttgtaaa attttattac caaattatta aacatctctt
      961 gtatttggaa gttgtgtttt ttacaaaaag ttttattcat aatatttttt atttcaattg
     1021 ctttaaaaag agcatcatct tttgaggaaa actcaaagct accatttaaa ttactattta
     1081 aaaaacattt acatttatct tgaattaata ttttatacat tattttacct ttaaatttta
     1141 aagtggaaag cttatcaaaa aatttattaa agcaacaaaa tagtaaaaaa aagttagaat
     1201 aaagtattaa ttattacggg ggaatatatg catattacaa atctactatc acaatatttt
     1261 ggtaaatttg cgaaaaaaga gtttccaaga cctattcaag agttgataaa tggtgcttat
     1321 acaaaattta tgaaattaga tttaaaagag tttaaaaatt caaaacatta caaatcttta
     1381 aatgaacttt ttacaagaga tttgataatc aaaagagaga tagatagctc aaaagatatt
     1441 tttatatcgc caactgatag tttaattaca gagtgtggaa agctaaaaaa tgatacagct
     1501 ttacaaataa aaggtatgga gtatagtgtt gaagagcttc ttacttatta ttgtagagat
     1561 aatttcttaa aattagaaaa tggtgaattt atgaattttt atttatcacc aagagattac
     1621 catagatacc atgcgccagt tgattttaag ttaaaaaaac taatccatgt acctggaaaa
     1681 ctatatcctg taaatttaaa atatctaaat aaagaacttg aactttttgt tcaaaatgaa
     1741 agagttgttt tagagtgtga aaaagatgga aaaatctttt atatggtttt tgttggagct
     1801 ttaaatgttg gtcaaatggt atttgaattt gagccaagag tggaaacaaa taaagataca
     1861 aaagagataa aagtttatac atatgagaat attgaaatct caaaagctga ctgtttaggt
     1921 tattttaaaa tgggttctac agttgtaatg atttgggaaa aagattttgt tgttttagaa
     1981 aacttattaa atcaaaatgt aaaatttggt caaaaaattg caaaatattg atttgctaaa
     2041 tactctctta tttgcaacaa catttttagc tctttttttt ataatttta
//
LOCUS       DAOQAN010000182         2087 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_78996_length_2087_cov_2.375984, whole genome shotgun sequence.
ACCESSION   DAOQAN010000182 DAOQAN010000000
VERSION     DAOQAN010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78996_length_2087_cov_2.375984"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2087
                     /locus_tag="PKO10_03655"
     CDS             <1..>2087
                     /locus_tag="PKO10_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66280.1"
                     /translation="RKSEINQRLRELNYRKEKMKYNLSENQDNDITLALKEYNQTMDK
                     LDQAEVGSREYSELSLASKTSFRRYLNEKTIDKEIRKNITESLELRIEKDKSPFKEEN
                     KEIIEKAIEDVKNYNIYQDLAKNKYTILGYTQKGGKKEEIDRPLVLKGDIPQYLVDYS
                     NMDNSSKGYIKLKLSKKQFSERLRDGILKSDDAKTIESKVLENYYKMKGNSYKERINM
                     LYPTERELKSFESVLLGQLAFVFQAKTIDTDATFMKPVSLSPENFKEVNGKPELSEDA
                     KAFNSKYKNELDNPSISIENGQIVAKIKDENGNDIVLDNNMYDINTLALIFGTKEKNA
                     FGTTANFVTVPDYAEEIMRTELIKELGNMYSVQYIDPDQNDGEDFNNIWGVDPLDDRA
                     EDLRYFVKDTFADKGYIPASVIIKSLGEKIYKSLPMSFDKANIEKDAEAKIANQLAIY
                     ALSAVSRKNGLGLSIYTSQQDLDSTELIQGKINDTMTANIPVATNINEATGEKATIVS
                     KLNLIRLDQDKAKNLIALSSILEYTDPSREGTMPSDKPIETTSDTIKNKTTPKSELAK
                     ETILDYQSVGYRIEKNIKELYDLWKNEKTRNFAYQIAGILNPDAKLNAREYRKKLSKN
                     KNERIQFDVLMRFFEEKGNDKEFYIKWDMIASGRYMEDSRITPQHNKITRVLLSTAGT
                     RTNIEFTKDEDGK"
BASE COUNT          892 a          294 c          350 g          551 t
ORIGIN      
        1 aaagaaaatc tgagataaat caaagactta gagagcttaa ctatagaaaa gaaaaaatga
       61 aatacaatct atctgaaaat caagacaacg acatcactct ggctctgaaa gaatataacc
      121 aaactatgga taagcttgac caagcagaag ttggtagcag agagtattct gagttatctc
      181 ttgcttcaaa aacttcattt agaagatatt tgaatgaaaa aactatagac aaagaaataa
      241 gaaaaaatat cacagaaagt cttgagttaa gaatagagaa agacaaatct ccatttaaag
      301 aagaaaacaa agagataata gaaaaagcta tagaagatgt aaaaaattac aacatatatc
      361 aagatttggc taagaataaa tatacaatac ttggatacac tcaaaaaggt ggaaaaaaag
      421 aagaaataga tagaccttta gtgctaaaag gagatattcc tcagtattta gttgattact
      481 caaatatgga taattcttca aaaggatata tcaaattaaa attgagcaaa aaacaattta
      541 gcgaaagatt aagagatgga atattaaagt ctgacgatgc aaaaactatt gaatcaaaag
      601 ttcttgaaaa ttactacaaa atgaaaggaa acagctacaa ggaaagaatc aatatgcttt
      661 atcctactga gcgtgagttg aaatcttttg aaagtgtatt gcttggccaa ttagcatttg
      721 ttttccaggc aaaaactata gatactgatg caacattcat gaagccagtt tctcttagtc
      781 ctgagaactt caaagaagta aatggaaaac ctgagctatc agaagatgca aaagcattta
      841 actctaaata taaaaacgaa ctagacaatc catctatatc aatagaaaat ggtcaaattg
      901 tagctaaaat aaaagatgaa aacggaaacg atattgttct tgataacaat atgtatgaca
      961 tcaatactct ggctcttata tttggaacta aagaaaaaaa tgcattcgga actactgcaa
     1021 actttgttac tgttcctgac tatgcagaag aaattatgag aacagaactt ataaaagagc
     1081 ttggaaatat gtattctgtt caatatatcg atccagacca aaatgatggt gaagatttta
     1141 acaacatctg gggagttgat ccacttgatg acagggctga agatttaaga tattttgtca
     1201 aagatacatt tgctgacaaa ggatatatcc cggcatctgt tataatcaaa tctcttggag
     1261 aaaaaatata taagtctctt ccaatgtctt tcgataaagc aaacattgag aaagatgcag
     1321 aagctaaaat agcaaaccaa ttagctatat atgcattgag tgcagtttct agaaaaaatg
     1381 gtctaggact aagtatatat acttctcaac aagacttaga cagtactgag cttattcaag
     1441 gaaagataaa cgatacaatg actgcaaata ttcctgtggc tacaaatata aacgaagcta
     1501 ctggagaaaa agcaacaata gtatctaaac ttaatttgat tagattagat caagacaaag
     1561 caaaaaactt aatagctctt tctagcattc ttgaatacac tgatccatct agagaaggaa
     1621 ctatgccttc tgataaacct attgagacta ctagcgatac aatcaagaac aaaactactc
     1681 ctaagtctga attagcaaaa gaaactattt tagattatca aagtgttgga tacagaattg
     1741 aaaaaaatat caaagagctt tacgatttat ggaaaaatga aaaaactaga aattttgcat
     1801 atcaaattgc tggaatttta aatccagatg caaaattgaa tgcaagagaa tacagaaaga
     1861 aactttctaa aaataaaaat gaaagaattc aatttgatgt tcttatgaga ttttttgaag
     1921 aaaaaggaaa cgacaaagaa ttctacatca agtgggatat gatagcttct ggaagatata
     1981 tggaagattc tagaattact cctcaacaca acaagataac tagggtccta ctatctactg
     2041 cgggaactag aacaaatata gagttcacaa aagacgaaga tggaaaa
//
LOCUS       DAOQAN010000183         2084 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_79141_length_2084_cov_4.521439, whole genome shotgun sequence.
ACCESSION   DAOQAN010000183 DAOQAN010000000
VERSION     DAOQAN010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79141_length_2084_cov_4.521439"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2067)
                     /locus_tag="PKO10_03660"
     CDS             complement(<1..2067)
                     /locus_tag="PKO10_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66281.1"
                     /translation="MQLNGKTVEEYEKQQKERLDKYNSSEEKFKRLEKTVNTLQLEKD
                     GLEKAVIAYEKSGASTEKIHSKLEKVNTELFNYRVEYEKLQNELADKQTAKDQLAQTE
                     QYGKLVTNIQIDIDRIKKDEKEILKTKGNIAEWESEITGYNKIKEESSKNLKKAEEAQ
                     AQAVKQSGKENLIISENIKNLKKDIANQDLLIALKQAKINEENQKDSDKTIRKSESEL
                     KRKTEINKKEAENKAVLLEIAAIQSGQIDEEKLKISLSELRIQSLEEEKKYLQDGYDK
                     NKLILEIEKEKLNLRKLQSKEYKFDQVSGLIKYTDMQNLGEIYQELKVYYKEDPESLS
                     DLEKWVEKEQNKLNKKPLTLQINLEGWDDISNSIATFGNSFQKIDKAQKKYQRENEKI
                     IKDEVELEQARIDLVDTTISGYGDMIGAIGSFYDEDDERRKKQLELQKVFSAAKMAMQ
                     VAELAQSTAFTSLFVAQETTKGAAAGATAVAVAAQSSPWTGFITAAAMAALLASFGIM
                     IGGKTKTTTVDMTMPVNDGKGSVLGDPMAQSESIANSLEILEDFARPQFRVLSEMNKN
                     VKTIADSIGGVASLLIKQGGFAFGEGFTPFSETKQNIKINNDIANAIVGGGVGLILSK
                     LKIPIISDIAGMFGGLVNSVMGGLFGKTSVKKTMTDSGIYFRKQLITDAINEMNGSAY
                     QTIKTKT"
BASE COUNT          488 a          366 c          252 g          978 t
ORIGIN      
        1 agtttttgtc ttaatcgttt gataagcact accattcatt tcatttatgg catctgttat
       61 tagctgtttt ctaaaataaa tcccactatc tgtcattgtt ttctttacag aagtttttcc
      121 aaaaagacca cccataacac tgtttaccaa cccaccaaac attcctgcaa tatcgcttat
      181 tatagggatt tttaattttg acaaaattaa tccaacacca ccaccaacta ttgcatttgc
      241 tatatcgtta ttgattttta tattttgctt agtttcacta aaaggcgtaa atccttcacc
      301 aaaagcaaaa ccaccttgtt ttatcaaaag agatgctact ccacctatac tatcggctat
      361 agtttttaca tttttattca tttcgcttag aactctaaat tgtggccttg caaaatcttc
      421 taaaatttcc aaactattag ctatgctttc gctttgagcc attgggtcgc caagaactga
      481 acctttgcca tcatttactg gcatagtcat atctactgtt gtagtttttg tttttccacc
      541 tatcattatt ccaaaagatg ctagtaatgc agccattgca gctgctgtta taaatccagt
      601 ccatggagaa gattgagctg ctactgctac tgctgttgct cctgctgctg caccttttgt
      661 tgtttcttgt gctacaaata gagatgtaaa tgctgttgat tgagctagtt ctgcaacctg
      721 catagccatt ttagctgctg aaaatacttt ttgtaattct aattgttttt ttcttctttc
      781 atcatcttca tcatagaagc ttcctatagc tcctatcata tctccatagc cagatatagt
      841 tgtatctact aaatcaattc ttgcttgttc tagttctacc tcatctttga ttattttttc
      901 attttctctt tgatattttt tttgagcttt atctattttt tggaaactat taccaaaagt
      961 tgctattgaa tttgatatat catcccaacc ttctaaattt atttgtaaag ttagaggttt
     1021 tttatttaat ttattttgtt ctttttctac ccatttttct aaatctgata aagattctgg
     1081 gtcttctttg taatatactt ttaattcttg gtatatttca ccaagatttt gcatatctgt
     1141 atatttaatc agtccagata cttgatcgaa cttatactct ttgctttgta attttcttaa
     1201 atttaatttt tctttttcta tttctaaaat taatttattt ttatcgtatc catcttgaag
     1261 gtattttttt tcttcttcta gtgactgtat tcttagctct gaaagagata tttttagctt
     1321 ttcttcatct atttgtcctg attgtattgc tgctatttca agaagtactg ctttattttc
     1381 tgcttctttt ttgtttattt ctgtttttct ttttagttcc gattcacttt ttcttatagt
     1441 tttgtctgaa tctttttggt tttcttcgtt tatttttgct tgttttagtg ctattaataa
     1501 atcttgattt gcaatatctt tttttaaatt tttgatattt tctgaaatta ttaaattttc
     1561 ttttccactt tgttttactg cttgggcttg tgcttcttct gcttttttta agttttttga
     1621 tgattcttct tttattttat tgtaaccagt aatttcgctt tcccattctg ctatattacc
     1681 ttttgttttt aatatttctt tttcatcttt tttaatacgg tctatatcta tttgtatatt
     1741 tgttactagc tttccatact gttctgtttg tgccaattgg tcttttgctg tttgtttgtc
     1801 tgctaattca ttttgtagtt tttcatattc aactctataa ttaaataatt cagtatttac
     1861 tttttctaac ttagagtgta ttttttcagt agaagcacca gatttctcat aagctattac
     1921 tgctttctct aagccatctt tttctaattg tagtgtgttt actgtttttt ctagtctttt
     1981 aaatttttct tctgaagagt tatatttatc taatctttct ttttgttgct tttcgtactc
     2041 ttctactgtt ttaccattta gttgcatttt tctgatttca tcat
//
LOCUS       DAOQAN010000184         2084 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_79147_length_2084_cov_3.392311, whole genome shotgun sequence.
ACCESSION   DAOQAN010000184 DAOQAN010000000
VERSION     DAOQAN010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79147_length_2084_cov_3.392311"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1246)
                     /locus_tag="PKO10_03665"
     CDS             complement(<1..1246)
                     /locus_tag="PKO10_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNW66282.1"
                     /translation="MISKELRHIFAQAINYARANKHEYLTLEHIFLMLINNSTIENMF
                     FDLGVDTKILFDDLKTYIEANTPKLPDDIKDDPIETVALSSTIEYMVAHTQSSGKTKA
                     NVEDMFVAILKDEKAYSTYLLKRLGIQRVDILEEISHKIDDENFDQKDEQNDKNEKIL
                     EQNSSELVSIAKKSKIDPVIGRDKELERVIEILGRRKKNNPLLVGEPGVGKTAIAEGL
                     ALKIANEEVPDFLKDSKIFSLDMGSMLAGTKYRGDFEKKLKSLLKEISKIPKAILFID
                     EIHTIVGAGSVGGSAMDASNILKPLLANGSIKCIGATTFLEYRNDFAKDKALSRRFAK
                     VDVNEPTIEDSILILEGLKSKYEEFHGVKYSKSAIISAVELSKKYIQDRFLPDCAIDV
                     IDEVGASKKINLSKTLKTKNEAN"
     gene            complement(1249..1548)
                     /locus_tag="PKO10_03670"
     CDS             complement(1249..1548)
                     /locus_tag="PKO10_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HNW66283.1"
                     /translation="MSIEIEIELNEDLEVEEPKKYNVFLLNDDYSTMDFVIDVLVKVF
                     RKNQTEAEAIMLEVHNNQKGLCGVYSFEIASTKVAQVKSLAREKGFPLKAIMEEE"
     gene            1634..2071
                     /gene="bioD"
                     /locus_tag="PKO10_03675"
     CDS             1634..2071
                     /gene="bioD"
                     /locus_tag="PKO10_03675"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF024891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HNW66284.1"
                     /translation="MKINPQDYIGKAIFITATNTNVGKTFASEVFLKYFAKNGLKVGY
                     FKPIETGVIDNSPLDGLKMFELVKKLNPNFLKLTINDIVPYQFTLPASPYVAKGNTFL
                     DIDFLIEKKEYLQTFCDVLIIEGAGGLFVPIKKDFLIMLAKAE"
BASE COUNT          673 a          347 c          226 g          838 t
ORIGIN      
        1 tatttgcttc attttttgtt tttaaagttt ttgatagatt gattttttta ctagcaccta
       61 cctcatctat tacatctatt gcacaatctg gcaaaaatct gtcttgaata tattttttac
      121 taagttcaac tgcgcttata attgcacttt ttgaatattt aacaccatga aactcttcat
      181 attttgattt taacccttcc aaaatcaaaa tagagtcttc aatagttggt tcatttacat
      241 caacttttgc aaatcttcta ctaagtgctt tatcttttgc aaaatcattt ctatactcca
      301 aaaatgtagt agctccaata cattttatac taccatttgc taaaagtggt tttaatatat
      361 ttgaagcatc catagcactt ccaccaacac ttccagctcc tacaattgta tgaatctcat
      421 ctataaatag tattgctttt ggaatttttg atatctcttt taaaagtgat ttgagttttt
      481 tttcaaaatc tcctctatat tttgttccag ctaacataga gcccatatca agagagaata
      541 tttttgaatc ttttaaaaaa tctggcactt cttcatttgc aatctttaaa gccaaacctt
      601 cagcaattgc agtttttcca acacctggtt ctccaactaa aagtggatta tttttctttc
      661 ttcttcctaa aatctcaatc actctttcaa gctctttatc tcttccaata actggatcga
      721 tttttgattt ttttgcaata cttacaagct cgcttgaatt ttgctctaaa attttttcat
      781 ttttatcatt ttgttcatct ttttgatcaa aattttcatc atctatttta tgagaaatct
      841 cttctaaaat atctactctt tgaattccca atctttttag taaatatgta gaatatgctt
      901 tttcatcttt taaaattgct acaaacatat cttcaacatt tgcttttgtt ttcccactac
      961 tttgagtatg agctaccata tactcaatag ttgaagacaa agctactgtt tctatagggt
     1021 catcttttat atcatctggc aattttgggg tatttgcttc tatatatgtt tttaaatcat
     1081 caaataaaat ctttgtatca acacctaaat caaaaaacat attttctatt gttgaattat
     1141 ttattagcat taaaaaaata tgctctaatg ttaaatattc atgcttattt gctcttgcat
     1201 aatttattgc ttgtgcaaaa atatgcctta actctttact tatcattttt actcttcttc
     1261 cattatggct tttaagggaa accctttttc tctagcaagg ctttttactt gtgcaacttt
     1321 tgtagaagca atctcaaaac tataaactcc acaaagacct ttttggttgt tgtgaacctc
     1381 tagcattatt gcttctgctt cagtttgatt ttttctaaat acttttacta aaacatctat
     1441 tacaaaatcc atagtagaat agtcatcatt taataaaaaa acattgtact tctttggctc
     1501 ttctacttct aaatcttcat ttaactcaat ttctatctca atactcactt aaaaccctta
     1561 attgtaaacc atattttaat atgttaatat cgctttttaa aaagcttaaa attataacaa
     1621 aaaagagatt attatgaaga ttaaccctca agattatata ggaaaagcaa tatttataac
     1681 agcaacaaat acaaatgtag gtaaaacatt tgcgagtgaa gtttttttaa aatattttgc
     1741 aaaaaatggt ttaaaagttg gatattttaa accaattgaa acaggagtta tagataactc
     1801 gccacttgat ggtctaaaaa tgtttgaact tgtaaaaaaa ctaaatccta attttttaaa
     1861 acttacaata aatgatattg tgccttatca atttactctt cctgcttcac cttatgttgc
     1921 aaaaggaaac acttttttag atattgattt tttaatagaa aaaaaagagt atttacaaac
     1981 tttttgcgat gttttaatta ttgaaggagc tggtggattg tttgtaccaa ttaaaaaaga
     2041 ttttttaatt atgttagcga aagctgaata attacctata aaat
//
LOCUS       DAOQAN010000185         2082 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_79245_length_2082_cov_2.971386, whole genome shotgun sequence.
ACCESSION   DAOQAN010000185 DAOQAN010000000
VERSION     DAOQAN010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79245_length_2082_cov_2.971386"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="PKO10_03680"
     CDS             <1..369
                     /locus_tag="PKO10_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014473838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNW66285.1"
                     /translation="LKDTNDKIVLLNFFTTWCPACKVEISNLVQIQETYKNDIVVVGI
                     LLEEFKTNEEINELLKSYNINYTVTNSSEAFDLAKSLGGIKAIPTIFLIDKHGTFFQK
                     YTGLVPNEMLEVDIKKLLER"
     gene            378..1316
                     /gene="ftsY"
                     /locus_tag="PKO10_03685"
                     /pseudo
     CDS             378..1316
                     /gene="ftsY"
                     /locus_tag="PKO10_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509071.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
     gene            1331..>2082
                     /locus_tag="PKO10_03690"
     CDS             1331..>2082
                     /locus_tag="PKO10_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66286.1"
                     /translation="MKRRTKNFVEDILIIIGVLFILYLIYFFVFSKDNDLSFETATKE
                     SKVEMETKDSKVFVSENGFLSQIYEDIKNKLFADNNELLTIKKVEEENKEDNTKNIYL
                     SQRAEILSKQEERVKEDLTTQQEQTTKNEENISTENRNIEALKQEEIKDKVVENVVEQ
                     RVENKLATEQKNEEPVQQIKDNDHKDETILLEPKTTLETKTLEDVKTDENSEEAHHEV
                     SNIDDFFDRFEKKVYSNIDRNFDKTTFKRGEFV"
BASE COUNT          893 a          204 c          342 g          643 t
ORIGIN      
        1 ttaaaagata caaatgataa aatagtttta ttaaacttct ttacaacttg gtgtccagct
       61 tgcaaagttg aaatttcaaa tttagttcaa attcaagaaa cttataaaaa tgatattgtt
      121 gtagttggaa ttttgcttga agagtttaaa acaaatgaag agataaatga gttgttaaaa
      181 tcttacaata taaactatac agtaacaaac tcaagtgaag cttttgattt agcaaaatca
      241 ttgggtggaa ttaaagcaat tcctactata tttttaatag ataagcatgg aacatttttt
      301 caaaaatata caggtttagt tccaaatgag atgttagaag ttgatattaa aaagctttta
      361 gagagatagg ataaaaaatg tttagttttc ttaaaaaaaa taaagataaa gataatcaag
      421 ttgttgaaaa tagtgaagta agtgagaact ttttttcaaa agcttttagt aaaacttttg
      481 cttcaataaa aagtgtagta cctcaaaaaa aagagaaaat atcttttgaa gagattgaag
      541 agattttaat tgaagcagat gttgaatatg aaattataga aaaagctatg aatggtttac
      601 cttctatgat ttcaagaaaa gagttaagac atagacttgt aatgcttttt gaacatgctc
      661 caaatgttga tttatcaaat attccaaaac cttatgtaag attgataatt ggtgtaaatg
      721 gtgctggaaa aactacaact atagcaaagt tagcagctaa attaaaaaaa gaggggcaaa
      781 gtgtaatttt aggagcagga gatactttta gagctgctgc tattgaacaa cttacttctt
      841 gggcaaataa acttgatatt ccaattataa aaacaaaaca aggtcatgat tcaagtgctg
      901 ttgcatacga tacaatttgt tcagcaattg ctagaaatat tgataatgtt attattgata
      961 cagcaggaag ccttcaaact caatcaaact aaaacaatga gtaaaaaaag atagtcaaag
     1021 tttgtgagaa agctttagaa aatagacctt atcaaaaact aatgatttta gatggaactc
     1081 aaggaaatag tgcaattgct caagcaaaag cttttaatga aattgtagga atagatggta
     1141 ttattgtaac aaaactagat ggaacagcaa aaggtggagc actattttct atatcaaatc
     1201 agcttgaact tcctattttt tatataggtg ttggtgaaaa acaagatgat ttaatagaat
     1261 ttagtccaga ttattttgta gatagtctgc ttgatgagat ttatattaaa gattaacata
     1321 agagggaaat atgaaaagaa gaactaaaaa ttttgttgaa gatatattaa ttattattgg
     1381 tgtattgttt attttatatt taatctattt ttttgttttt tcaaaagata atgatttatc
     1441 atttgaaaca gcaacaaaag agagtaaagt agaaatggaa acaaaagatt caaaagtatt
     1501 tgtttctgaa aatggttttt tatctcaaat ctatgaagat ataaaaaata aactctttgc
     1561 agataataat gaacttttaa ctataaaaaa agttgaagag gaaaataaag aagataatac
     1621 aaaaaatatc tatctttctc aaagagctga aatattatca aaacaagaag agagagtaaa
     1681 agaggattta actacacaac aagaacaaac tactaaaaat gaagaaaata tctctacaga
     1741 aaatagaaat attgaagctt taaaacaaga agagataaaa gataaagttg ttgaaaatgt
     1801 agttgagcaa agagttgaaa ataaattagc tacagagcaa aaaaatgaag agccagttca
     1861 acaaattaaa gataatgatc ataaagatga aactatttta cttgaaccaa aaactacatt
     1921 agaaacaaaa actttagaag atgtaaaaac agatgagaac agtgaagaag ctcatcatga
     1981 agtatcaaat attgatgact tttttgatag atttgagaaa aaagtttata gtaatataga
     2041 tagaaatttt gataaaacaa cttttaaaag aggtgaattt gt
//
LOCUS       DAOQAN010000186         2082 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_79259_length_2082_cov_2.273310, whole genome shotgun sequence.
ACCESSION   DAOQAN010000186 DAOQAN010000000
VERSION     DAOQAN010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79259_length_2082_cov_2.273310"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..711)
                     /locus_tag="PKO10_03695"
     CDS             complement(52..711)
                     /locus_tag="PKO10_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014474277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNW66287.1"
                     /translation="MKNLKVLIVEDEQKLANLIRNSIKELFFKVAIAKDGIDGIKKFK
                     SFKPDIVISDISMPNLSGLEMCKIIKSNSQTPIIILSAYSQKEMLLEAIDLGISKYFI
                     KPFDIESFIEYLKELSNKVNKEKDYTLKDGFVFVKNSCSLYKDSELINLTKREKEFIN
                     LLIENKNLLVKMETIKENLWELENISDERVRTFIKRLRAKTSKDLIENVSSQGYMITS
                     N"
     gene            complement(708..1790)
                     /locus_tag="PKO10_03700"
     CDS             complement(708..1790)
                     /locus_tag="PKO10_03700"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HNW66288.1"
                     /translation="MNNYQKAIENSNIVSKTDINGIITFVNDEFCNLFGFTKDELIGK
                     NHNIIRHPDTPKENFELLWNTILDKKVYKSTVKNISKNGEDIYLNTTIIPILDEKEEN
                     IVEFVAIRYDITNEVLLQKELEEKQKIIFLQSRMASLGQMLGNIAHQWRQPLTELNLS
                     LFNLKKAFENENKKEIENLYSNSKNLILGMSSTIDDFTNFFAPKKQKEAFLINQSINE
                     ALKILNRVLEDENINIKFDILKDLEIFGLKNELTQVLLNLINNSKDAFVQKNITNKEI
                     TIKTYTNTKDDFVNLEYIDNAQGLSKELFDKIYEPYFTTKHQSIGTGLGLFICKIVIE
                     NSFKGEIMHENISNGLKFTLKFPKIV"
     gene            complement(1853..>2082)
                     /locus_tag="PKO10_03705"
     CDS             complement(1853..>2082)
                     /locus_tag="PKO10_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013136004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HNW66289.1"
                     /translation="IATQSGSNEEIEKYLKDKNLSFKVVNDEYGLLSTEFNVKAFPTT
                     FIYDKNKNLKFTEVGYTSTFGLKLRLWWSSF"
BASE COUNT          679 a          292 c          205 g          906 t
ORIGIN      
        1 aaaaagattt tatcaaaact aatttattct atgatttaaa caaataaaaa atcaattaga
       61 tgttatcata tacccttgag atgatacatt ttcaattaaa tctttagatg tttttgctct
      121 taatcttttt ataaaagttc taactctttc atcacttata ttctctaatt cccataaatt
      181 ttctttaata gtttccattt ttacaagtaa atttttattt tctattagaa gatttataaa
      241 ctctttctct ctttttgtaa gatttattag ttcactatct ttgtataaac tacaagagtt
      301 ttttacaaat acaaaaccat cttttaaggt atagtctttc tccttattta ccttatttga
      361 aagctctttt aagtattcaa taaagctttc aatatcaaat ggttttataa aatattttga
      421 aattcccaaa tcaatagctt ctaaaagcat ctctttttgg ctgtaggcac ttaaaattat
      481 tataggagtt tggctattac tttttattat tttgcacatt tcaagaccac ttaaattggg
      541 catagaaata tcactaatta caatatcagg tttaaaactt ttaaactttt taattccatc
      601 aataccatct tttgcaattg ctactttaaa aaaaagctct tttatagagt ttcttattaa
      661 atttgctaat ttttgctcat cttctactat taaaactttt agatttttca tactattttt
      721 ggaaacttta gagtgaattt taaaccattt gaaatatttt catgcataat ttcaccttta
      781 aaactatttt caataactat tttacaaata aaaagaccaa gacctgttcc aattgattga
      841 tgttttgttg taaaatatgg ttcataaatc ttatcaaata gctccttgct taatccttga
      901 gcattatcaa tatattctaa atttacaaaa tcatcttttg tatttgtata agtttttata
      961 gttatctctt tatttgtgat atttttttgt acaaaagcat cttttgagtt attaataaga
     1021 tttaatagta cttgagttag ctcatttttt aatccaaaaa tctctaaatc ttttaaaata
     1081 tcaaacttta tattaatatt ttcatcttct aagactctat ttaaaatctt caaagcttca
     1141 tttatacttt gatttattaa aaaagcttct ttttgttttt ttggagcaaa gaaatttgta
     1201 aaatcatcaa tagtacttga cattcctagt attagatttt ttgagtttga ataaagattt
     1261 tcaatctctt ttttgttctc attttcaaaa gcttttttta gattaaataa gcttaagttt
     1321 aactctgtta aaggttgcct ccactgatga gcaatatttc ctaacatttg acctaaactt
     1381 gccattcttg attgtagaaa tataattttt tgtttctctt ctagctcttt ttgaagtagt
     1441 acttcatttg taatatcata tcttatggct acaaactcaa caatattttc ctctttttca
     1501 tctaaaatag gaataatagt tgtatttaaa taaatatctt ctccattttt tgatatattt
     1561 tttacagtag atttatagac ttttttatct aaaatagtat tccaaagcag ttcaaaattc
     1621 tcttttggag tatcaggatg tcttataata ttgtgatttt ttccaattaa ttcatctttt
     1681 gtaaaaccaa aaagattaca aaattcatca tttacaaaag tgataattcc atttatatct
     1741 gtttttgaga ctatatttga attttctatt gctttttggt agttgttcat aagtttaaaa
     1801 tcctataaaa atttttctat cctatcaaag aagctttttt ttgtcaatga ttttagaaac
     1861 tactccacca aagcctaagt tttaatccaa aagttgaagt gtaaccaact tcagtgaact
     1921 ttagattttt gtttttgtca taaataaaag ttgtagggaa tgcttttaca ttaaattcag
     1981 tagataaaag accatattca tcatttacga ctttgaagct aagattttta tcttttaaat
     2041 atttttctat ctcttcatta cttcctgatt gtgtagctat tg
//
LOCUS       DAOQAN010000187         2077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_79538_length_2077_cov_2.602868, whole genome shotgun sequence.
ACCESSION   DAOQAN010000187 DAOQAN010000000
VERSION     DAOQAN010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79538_length_2077_cov_2.602868"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(19..366)
                     /locus_tag="PKO10_03710"
     CDS             complement(19..366)
                     /locus_tag="PKO10_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66290.1"
                     /translation="MKVKGFGKKYFTLASIQAYATQSKLHAKFTALKTNINEKLKISS
                     NSDEEDELSCDIEQSLLSCSIVNSKPCHCDCFFKRKTALAVRLFQFIPRCPYYTTYFA
                     FRRTGVHPPQLSF"
     gene            477..1154
                     /gene="trmD"
                     /locus_tag="PKO10_03715"
     CDS             477..1154
                     /gene="trmD"
                     /locus_tag="PKO10_03715"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011980064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HNW66291.1"
                     /translation="MKFTFVTLFPNLIEPYLKDSILNRAVESNLISYDFYNPRDFTTN
                     KHKKVDDAMVGGGAGMLLSCQPLFDCLEEIKRKDKEAYIIFPLAAAKPFRQNDAKRLA
                     NKKNIVMVSGRYEGIDERVIEKYANEVFSIGEYILTGGELPSLVMADAISRNVQGVLG
                     NEASLDVESYENNLLEAPSFTKPENYENLFVVKEYLKGNHSRICDLKFQMSICRTKYY
                     RPNKERR"
     gene            1151..1507
                     /gene="rplS"
                     /locus_tag="PKO10_03720"
     CDS             1151..1507
                     /gene="rplS"
                     /locus_tag="PKO10_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002787409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HNW66292.1"
                     /translation="MKNRYIASFEAAQIASKEVPAFRAGDTLRLGVEIKEGEKKRVQT
                     FEGVVIGRSGNGVDATFTIRKLGANNIGVERIFPLYCESLQSIEVLRKGDVRRAKLNY
                     LRALKGKAAKIRELKR"
     gene            1595..>2077
                     /locus_tag="PKO10_03725"
     CDS             1595..>2077
                     /locus_tag="PKO10_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HNW66293.1"
                     /translation="MLSQIINFIVETVGQLGYLGIFIMMFLESSFFPFPSEVVMIPAG
                     YLAYKGEMNMYLVILFGILGSLAGAIFNYYFALKLGRRFLMRYGKYILISEDTILKME
                     EFFNKHGHISTFFGRLIPVVRQYISLPAGLSRMNLFVFSLFTSLGAGIWVAILAFLGY
                     F"
BASE COUNT          746 a          250 c          364 g          717 t
ORIGIN      
        1 aatacaaaac aataattatt aaaatgataa ttgtggtgga tgtacgccag ttcttctaaa
       61 tgcaaaatat gttgtatagt atgggcatct agggataaat tgaaaaagtc ttacagcaag
      121 tgctgttttt cttttaaaaa agcaatcaca atgacaaggt ttagaattta ctatactaca
      181 agataataaa ctctgttcaa tatcacagga taactcatcc tcttcatcac tatttgaaga
      241 gatttttagt ttttcattta tattagtttt tagtgcagta aattttgcat gcaattttga
      301 ttgtgttgca tacgcttgaa ttgaagctaa tgtaaaatat ttttttccaa agcctttaac
      361 tttcaaaaat ttactcctta tattattaga tgcgaaattt atactattta tacttaataa
      421 tatataaaaa aatttatgat aacatttcaa acaaaaaaat tgattggaat tttattttga
      481 agtttacttt tgttacactt tttcccaatt tgattgaacc atatttaaaa gattctattt
      541 taaatagagc agttgaatca aatttaataa gctatgattt ttataatcct agagatttta
      601 caacaaacaa gcacaaaaaa gttgatgatg ctatggttgg tggaggagct ggaatgcttc
      661 tatcttgtca accacttttt gattgtttag aagagataaa aagaaaagat aaagaggcat
      721 atattatttt tccacttgcg gctgctaaac cttttaggca aaatgatgca aaaagacttg
      781 caaataaaaa aaatattgta atggttagtg gaagatatga gggaattgat gagagagtta
      841 ttgaaaagta tgccaatgag gtttttagca ttggagagta tatcttaaca ggtggagagc
      901 ttccatcact tgttatggct gatgcaatat ctagaaatgt tcaaggagtg cttggaaatg
      961 aggcttcttt ggatgttgag agttatgaaa acaatttatt agaagcacct tcttttacaa
     1021 aaccagaaaa ttatgaaaat ttatttgtcg ttaaagaata tttaaaggga aaccatagta
     1081 gaatttgcga cttaaaattt cagatgtcca tttgcaggac gaaatactat agacccaata
     1141 aggaaagacg atgaaaaaca gatatatagc aagttttgaa gcagctcaaa tagcttctaa
     1201 agaagttcca gcgtttagag caggagatac attaagactt ggtgttgaga ttaaagaggg
     1261 tgagaaaaaa agagttcaaa ctttcgaagg tgtagtaatt ggaagaagcg gaaacggtgt
     1321 tgatgctaca tttactatta gaaaacttgg agcaaataat attggtgttg agagaatttt
     1381 cccactatat tgtgagtctt tacaatctat cgaagtatta agaaaaggtg atgtaagaag
     1441 agctaaactt aactacttaa gagcactaaa aggtaaagct gcaaaaatta gagagctaaa
     1501 aagataataa ttaaggctta tgccttaatt tttcttcaaa taaactctct aaaaacttca
     1561 aaatcttttt taaccattca aaaaaaggaa aactttgtta tcacaaataa ttaacttcat
     1621 tgtagaaact gttggtcagt taggatattt aggtattttt ataatgatgt ttttagagag
     1681 ttcttttttc ccattcccat cagaagttgt aatgattcca gctggatatt tagcttataa
     1741 aggtgagatg aatatgtacc tagtaattct ttttggaatt ttaggttcgc ttgcaggtgc
     1801 aatatttaat tactattttg ctttaaagct tggaagaaga tttttaatga gatatggaaa
     1861 atatatcttg attagcgaag atacaatatt aaaaatggaa gagtttttta ataaacatgg
     1921 tcatatctca acattttttg gaagattaat tccagttgta agacaatata tctctttacc
     1981 agcaggactc tctcgaatga atctttttgt attttcacta tttacaagtt taggtgctgg
     2041 aatttgggtt gctatattag catttttagg atatttc
//
LOCUS       DAOQAN010000188         2073 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_79750_length_2073_cov_5.023786, whole genome shotgun sequence.
ACCESSION   DAOQAN010000188 DAOQAN010000000
VERSION     DAOQAN010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79750_length_2073_cov_5.023786"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            124..>2073
                     /locus_tag="PKO10_03730"
     CDS             124..>2073
                     /locus_tag="PKO10_03730"
                     /inference="COORDINATES: protein motif:HMM:NF017673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HNW66294.1"
                     /translation="MAIFKKIGKRLSKAYFKESKMTHEEWATKKVYFPSQDISPILGY
                     FKIKFSPQYKLLFKLMDRPDVHTLFAKWASQSGKSLFEMLIIGYRLDNEPANVLYMQP
                     IKDDIPKVLRLKINPVLKCIKDLWQKFEDYIATEGIRSKDAIKQLAGGSLVVSGSSVK
                     ERKSLTVPLLVMDEIGEFDKGAVKEASERLKAFSKFFPKKIGVSTIVHPKDEICTNFE
                     NCEAIMEWRYKCPNCKDNFYPSRETFKFMTKKEFADLNNIPENEVEKSSWKKEALKNV
                     YIECPHCKYQINNKEKSNLILNGGMDWFFIRGDENAKSFGADMNSLGSYFVELEDLAE
                     LLIDADGSIVDLDAIYRGWFNSFYQSKTSNKSRSEILSLGNGVSQWIIPKDTIPHGKY
                     LTVDTQKDHFWYTIYAFQYGAIQSLVSYGKIYDFNEIERLFEREYTDEDGNKMYISKL
                     AIDYQGYVKKETIEDDDGNQIVIEDIDRMEEVKTWAVGYMRKMGYDRIYLTRGVEELT
                     NQTEFSEFKIKVTDQLIGKEVDIRAWKMSNLRCSSVLDQKIQRSIEKIKSEAPDSYKD
                     QLFYIYQDIIDILSNEEHKSVATDLDRQLRSEVLKYKDSKAKKMTYHRISRENHIWDT
                     SKMAVMLSIIDNLAPIPKPKKIEKSI"
BASE COUNT          880 a          265 c          358 g          570 t
ORIGIN      
        1 caatcacggt tggtatgata aaaaaatttt atacaaagag cataggcaaa ttttatttaa
       61 taatattgaa tgtcaaagag cagtaagaca aaataaaaaa acaagcaaag gtttatttga
      121 ttaatggcaa tatttaaaaa aataggaaaa agattatcta aagcttactt caaagaatca
      181 aaaatgacac acgaagagtg ggcgaccaaa aaagtatatt ttcctagtca ggatatatct
      241 ccaattttag gatattttaa aataaaattt tcaccacaat acaaattgtt atttaaacta
      301 atggacagac cagatgttca tactttattc gcaaagtggg catcacagtc gggtaaatct
      361 ttatttgaaa tgttaattat agggtatagg ctagacaatg aaccagcaaa tgttttatat
      421 atgcagccaa taaaagatga tattccaaaa gttctaagac taaaaataaa tccagttcta
      481 aagtgcatta aagatttatg gcaaaaattt gaagactata tagcgacaga gggaataaga
      541 agtaaagatg caattaaaca actcgctggt ggaagtttag tcgtatcagg ttcaagtgta
      601 aaagagagaa aatcactaac agttcctctt ttagtaatgg atgagattgg agaatttgac
      661 aaaggtgcag taaaagaagc aagtgaaaga ttaaaagcct tttctaaatt tttcccaaaa
      721 aaaataggag tttctacaat agttcaccca aaagatgaaa tctgtacaaa ctttgaaaac
      781 tgcgaagcaa ttatggagtg gagatataaa tgcccaaact gtaaagataa cttctatcct
      841 tcaagagaaa cttttaaatt tatgactaaa aaagaatttg cagacttaaa taatattcca
      901 gaaaatgaag tagaaaaatc atcatggaaa aaagaagctc taaaaaatgt atatatagaa
      961 tgtccacatt gcaaatatca aataaacaat aaagagaaat caaatctaat actaaatggt
     1021 ggaatggatt ggttttttat aagaggtgat gagaatgcaa aatcatttgg tgcagatatg
     1081 aattctttgg gttcttactt tgttgagtta gaagatttag cagaacttct tatagatgca
     1141 gatggcagca tagttgattt agatgcaata tacagaggat ggtttaatag tttctatcaa
     1201 tcaaaaactt caaacaaatc aagaagtgaa atactatctc ttgggaatgg agtatctcaa
     1261 tggataattc caaaagatac aataccacat gggaaatatt tgacagtaga tactcaaaaa
     1321 gaccactttt ggtacacgat ttatgctttc cagtatggag caatacagag tttagtttct
     1381 tatggaaaaa tatatgattt taatgaaata gaaaggcttt ttgaaagaga atatacagat
     1441 gaagatggaa ataaaatgta tatctcaaaa ctagctattg actaccaggg atatgtaaaa
     1501 aaagaaacaa tagaagatga tgatggaaac caaatagtaa tagaagatat agacagaatg
     1561 gaagaggtaa aaacttgggc agttggatat atgagaaaaa tgggatacga tagaatatat
     1621 cttacaagag gtgttgaaga actcacaaac caaacagagt tttcagagtt taaaataaaa
     1681 gttacagacc aacttatagg caaagaagtt gatattagag cttggaaaat gtcaaacctt
     1741 agatgttcct ctgttttaga tcaaaaaatc caaagaagta tagagaaaat caaatcagaa
     1801 gcaccagaca gttataaaga ccaactattc tatatatatc aagatattat agatatatta
     1861 tcaaacgaag aacacaaatc tgtagcaaca gaccttgaca gacaactccg ttcagaagtt
     1921 ttaaaatata aagactctaa ggctaaaaaa atgacttatc atagaatttc aagagaaaat
     1981 cacatatggg atacctctaa aatggcagtt atgctttcta taattgacaa tttagcacct
     2041 attccaaagc caaaaaaaat agaaaaatca ata
//
LOCUS       DAOQAN010000189         2072 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_79817_length_2072_cov_3.054536, whole genome shotgun sequence.
ACCESSION   DAOQAN010000189 DAOQAN010000000
VERSION     DAOQAN010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79817_length_2072_cov_3.054536"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /gene="infC"
                     /locus_tag="PKO10_03735"
     CDS             complement(<1..318)
                     /gene="infC"
                     /locus_tag="PKO10_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HNW66295.1"
                     /translation="MSRDNKKSDVIMNEDITAKELRCTSDSGENHGIIPTAQALALAD
                     EAGLDLVLIAADANPPVAKIMDYSKFKYQQEKKKKEAKKNQKIVVVKEIKLSVKIADN
                     DINY"
     gene            complement(315..>2072)
                     /gene="thrS"
                     /locus_tag="PKO10_03740"
     CDS             complement(315..>2072)
                     /gene="thrS"
                     /locus_tag="PKO10_03740"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012011979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HNW66296.1"
                     /translation="AEALNIKGDIIKADDSKESLEILRHSCAHMMAQAIKELYPEAKF
                     FVGPVVKEGFYYDFKVESKISDEDLPTIEKKMKEIADRKLPITRHETTKEEFYEKFKN
                     DELKQAVLKNIKDDTLTIYKQGDFEDLCRGPHLPNTRMIRSFKLTRVAGAYLGGDEKN
                     EMITRIYGIAFFDKQALFDYTKMIEEAKKRDHRKLGTELELFTFNNDVGAGLPMWLPN
                     GARLRSKLEHLLYKAHRVRGYEPVRGPEILKSEMWKISGHYANYKENMYFTTIDDQEY
                     GIKPMNCVGHIQIFKNSLVSYKDLPKKLFEYGVVHRHEMSGAMHGLFRVREFTQDDSH
                     IFCTQDQIKDVIFEVLEFVDSLLKMFDFKYEIEVSTKPEKAIGDDIFWEKTTAGIMDA
                     LNEKNIEYGIDEGGGAFYGPKIDIKILDAIGRKWQCGTVQVDMNLPSRFNAEFINDKG
                     EKEQPVMIHRAILGSFERFIGILTEHCAGEFPFVIAPTQVIFVPIADSHVEYAKEIQR
                     DLVEDGIDSKIFNMNESLNKRIRMAEKERVPMIVVIGDEEVANKSIALRNRRTREQSN
                     LSKQDFIDLLNKINEESRI"
BASE COUNT          618 a          396 c          241 g          817 t
ORIGIN      
        1 gtagttaata tcattgtcag caatttttac agatagcttt atctctttaa caacaactat
       61 tttttgattt ttctttgctt cttttttctt tttttcttgt tgatatttga atttactgta
      121 gtccataatc tttgcaacag gaggattagc atctgcagct ataagaacta aatcaagtcc
      181 agcttcatct gcaagagcaa gagcttgtgc agttgggata atcccatgat tttctccact
      241 atcgcttgta catcttaact cttttgcagt aatatcctca ttcattatta catctgattt
      301 tttattatct ctactcaaat tctactctcc tcgtttattt tgtttagtaa gtcaataaaa
      361 tcttgcttac ttaaatttga ttgctctcta gttcttctat ttcttaaagc aatagattta
      421 ttagcaactt cctcatctcc aataacaaca atcattggaa ctctctcttt ttctgccatt
      481 ctaattcttt tatttaaact ttcattcata ttaaagattt tagaatctat accatcttct
      541 actaaatctc tttgtatctc ttttgcatac tcaacatgag aatcagcaat aggaacaaaa
      601 ataacttgag ttggtgcaat aacaaatgga aattctccag cacagtgttc agttaaaatt
      661 ccaataaatc tttcaaatga acctaaaatt gctctatgaa tcataactgg ttgttctttt
      721 tcacctttgt cattaataaa ttcagcatta aatcttgaag gcaaattcat atctacttga
      781 acagtaccac attgccattt tctaccaatt gcatctagga ttttaatgtc gatttttgga
      841 ccataaaatg ctcctccacc ttcatcaatt ccatactcta tatttttttc atttaaagca
      901 tccataattc cagcagttgt tttttcccag aaaatatcat cgccaattgc tttttcaggt
      961 tttgtagaaa cttcaatttc atatttaaaa tcaaacattt taagaagtga atcaacaaac
     1021 tctaatactt caaaaattac atcttttatc tgatcttgtg tacaaaagat atgtgaatca
     1081 tcttgagtaa actctcttac tctgaataat ccatgcatag ctccactcat ttcgtgtcta
     1141 tgtacaactc catattcaaa aagttttttt ggtaaatctt tatatgaaac taaactattt
     1201 ttaaagattt gaatatgccc aacacagttc attggtttaa ttccatactc ttgatcatca
     1261 atagtagtaa aatacatatt ctctttatag tttgcgtaat gccctgagat tttccacatt
     1321 tctgatttta atatctcagg tcctcgaact ggttcataac ctctaactct gtgagcttta
     1381 tataaaagat gctctaactt acttcttagt cttgctccat ttggtaacca cataggaaga
     1441 ccagctccaa catcattatt aaatgtgaat agctcaagtt cagttcctag ttttctgtgg
     1501 tctctttttt tagcctcttc aatcattttt gtataatcaa ataaggcttg tttatcaaaa
     1561 aatgcaatac cataaattct agtaatcatc tcattctttt catcaccacc aagatatgca
     1621 cctgcaactc gtgtaagttt aaaacttctt atcattcttg tatttggtaa gtgaggacct
     1681 ctacataaat cttcaaaatc accttgtttg tagattgtta aagtatcatc ttttatattt
     1741 ttaagaacag cttgttttaa ctcatcattt ttgaatttct cataaaactc ttcttttgta
     1801 gtctcatgtc tagtaatagg tagttttcta tctgctattt ctttcatttt cttttcaata
     1861 gttggtaaat cttcatctga aattttactt tctactttaa agtcataata aaaaccttct
     1921 tttacaacag gtccaacaaa gaattttgct tcaggataaa gttctttgat agcttgagcc
     1981 atcatgtgag cgcaagagtg tcttaaaatc tctagagact cttttgagtc gtcagctttt
     2041 ataatatctc ctttgatatt taaagcttca gc
//
LOCUS       DAOQAN010000190         2072 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_79836_length_2072_cov_2.125434, whole genome shotgun sequence.
ACCESSION   DAOQAN010000190 DAOQAN010000000
VERSION     DAOQAN010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79836_length_2072_cov_2.125434"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..150)
                     /locus_tag="PKO10_03745"
     CDS             complement(<1..150)
                     /locus_tag="PKO10_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNW66297.1"
                     /translation="MKKPIVVGSVAYDPKIVTIWDIIRDYFNDNGVRLDYVLFSNYEA
                     QIEYLL"
     gene            complement(160..1287)
                     /locus_tag="PKO10_03750"
     CDS             complement(160..1287)
                     /locus_tag="PKO10_03750"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNW66298.1"
                     /translation="MSNLYNEMIEFAKDNIAPFTTQVDAEAKFPVDSFKAIKDKKLTG
                     LLVPKEFGGMGFGFYEHTQTVLAFANYCATTALCYMMHNVATNCLATHGSEELKKEFL
                     PKIANGEIMLALAYSESGTGTHFYNPEIKVTKDGQTLSMNGRKSFVTSAQYADYYLID
                     ANSFDSEGLDNWLVSKDLAGINFEHSAWNGLGMRGNASCPMILENVKIDERFRIGAAG
                     SGLDQIFNTVGPFFIMGLAAVYSGVALNASNSIIDYSMNRKYSDNSALCGIPTVQNHI
                     SDIYSKAASAKYFTLSAAKSVIEADPMAQANVLAARIHASSMAVDVCTTAMKIGGGTA
                     YAKRINIERLLRDSLAAAVMAPSTDVLTTWLGKALTNQEII"
     gene            complement(1299..2072)
                     /locus_tag="PKO10_03755"
                     /pseudo
     CDS             complement(1299..2072)
                     /locus_tag="PKO10_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008335273.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
BASE COUNT          663 a          380 c          238 g          791 t
ORIGIN      
        1 taataaatac tcaatttgtg cttcataatt tgaaaaaaga acataatcaa gtcttacacc
       61 attatcatta aaataatctc ttatgatatc ccaaatcgtt acgatttttg ggtcataagc
      121 aactgaacct acaactattg gttttttcat tttttctcct taaattattt cttgatttgt
      181 taaagctttt ccaagccaag ttgtaagaac atcagtactt ggtgccataa cagcagcagc
      241 tagagaatct cttaaaagtc tttctatatt tattcttttg gcataagcag ttcctccacc
      301 aatcttcata gcagttgtac aaacatcaac agccatagat gaagcatgaa ttcttgctgc
      361 taatacattt gcttgtgcca ttgggtctgc ttcaattaca ctttttgctg cacttaatgt
      421 aaagtatttt gcacttgcag cttttgaata gatatctgaa atatgatttt gaacagttgg
      481 aataccacaa agtgctgaat tatcactata ttttctattc attgagtaat caattattga
      541 attacttgca tttaatgcaa ctccactata aacagctgct aatcccatta taaaaaatgg
      601 accaaccgta ttaaatattt gatctaatcc acttcctgct gctccaattc taaatctttc
      661 atcaattttt acattttcta aaatcattgg gcaagaagca tttcctctca ttccaagtcc
      721 attccaagca ctatgttcaa aattaattcc tgctaaatct tttgaaacca gccaattatc
      781 tagtccttca ctatcaaatg agtttgcatc aattaaataa taatctgcat attgtgctga
      841 tgtaacaaaa ctttttcttc cattcatact taaagtttgt ccatctttag taactttgat
      901 ttctggatta taaaaatgag ttcctgtacc actttcacta tatgcaagtg ctaacataat
      961 ttcaccattt gcaatttttg gtaaaaactc tttttttaac tcttcacttc catgagttgc
     1021 taaacaattt gtagcaacat tatgcatcat ataacacaaa gctgttgttg cacaataatt
     1081 tgcaaatgct aaaacagttt gcgtatgttc ataaaaacca aatcccattc ctccaaactc
     1141 ttttggaact aaaagacctg ttaatttttt atcttttata gctttaaaac tatcaactgg
     1201 aaattttgct tccgcatcaa cttgtgttgt aaatggtgca atattatctt ttgcaaactc
     1261 tatcatttca ttatataaat ttgacatttt ttttcctact acataaaatt tggtgtgtgt
     1321 gcttttgaaa tatcattcgc taaatcttca tcaataccta tactaactaa tctttttatc
     1381 ctattttcat tcattttttg ttttaaatct tcaactaaat caaatccttt tttatagtca
     1441 tttggctcat cgcttccacc tgcaagaatt gctaaagatt caagtaataa agatgattca
     1501 ctacttattt catccataaa ctcttttctt ttaaaagcag tgttttttag agcatttatc
     1561 ctatttggat tttgttcaat cgcagatttt acatgttcaa tcccataagc aacatgtctc
     1621 atttcatctc tttttgatag tcttataatc ttttttgtag cttcatctgg catatatttt
     1681 tctaaaaaag ttaataaatc aacaaaagtt ccttctccca taacatgaag taaaaaagag
     1741 cttttaatat aatcatcttc tttaaataat gagaaaagtg aacgctgagt aacttcactt
     1801 gaatattgaa atcctcacca tttgcattag cacgtttagt aaatacttca atgtgtcttg
     1861 cttcatcatt cattagtgat gataaaaaaa gtggaacttc catataatat ggatttattt
     1921 tacttataaa ttttccagga atatataaag ctgaaaattc attttcaact aaatatgtca
     1981 taatctgaca aattgccttt tctaaaactg gattaaactc aggaatttca ttccaaggaa
     2041 tatctgttgt tgcattccat tgtaaatttt tt
//
LOCUS       DAOQAN010000191         2072 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_79841_length_2072_cov_1.942489, whole genome shotgun sequence.
ACCESSION   DAOQAN010000191 DAOQAN010000000
VERSION     DAOQAN010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79841_length_2072_cov_1.942489"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="PKO10_03760"
     CDS             complement(<1..255)
                     /locus_tag="PKO10_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014469361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNW66299.1"
                     /translation="MLSKKKLIIFPTQRAIRNYLEKKKSLNTLLPTCLTIDDFLKKSI
                     YLENKIYCDEEQRVLLLSEAIKDIDIKKLGISSSFTKFLTQ"
     gene            complement(245..805)
                     /locus_tag="PKO10_03765"
     CDS             complement(245..805)
                     /locus_tag="PKO10_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66300.1"
                     /translation="MKKRNYWPLYFIAIFAFTVYMIIWTIYKATQVPVVEDRSFMQKY
                     QYVDENYNNIMTSNINFLEKYSLELDLNGKIFPLTTEDIKYGQRVIEKYSNHKDVLKV
                     GENSLKISVLNKITKEIVPINIDLLITKTMSDDSNLNLKDENFTKDKNIYTTNFSLNE
                     ETNWIITGSFKVEDETGFIFIKTNAK"
     gene            complement(807..1433)
                     /locus_tag="PKO10_03770"
     CDS             complement(807..1433)
                     /locus_tag="PKO10_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014475275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66301.1"
                     /translation="MKNFNSLKVGVIFSLLLFLYTNGFAQNFILKDDLIDNRAKDKIV
                     EIGNEVKTKTGVNIYVYSTSSFNLDKDISTKDKIDYIKNYELDILKNLETPYVIITIS
                     LEDTHVNLYYSDTLEKIIDKNEILDDYVVPLLASKDKNTVASKVSASVLNGYAAVADK
                     LADSKNIELESNIGNQGRVASTIWRVFIYFVLLSALLAYIYAILRKRK"
     gene            complement(1537..1779)
                     /locus_tag="PKO10_03775"
                     /pseudo
     CDS             complement(1537..1779)
                     /locus_tag="PKO10_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510965.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4006 family protein"
     gene            complement(1779..>2072)
                     /locus_tag="PKO10_03780"
     CDS             complement(1779..>2072)
                     /locus_tag="PKO10_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNW66302.1"
                     /translation="MMLSEDADIKEVSAYVANGFKGEQPAAYATCATCHGDNGEGMPM
                     VGPNIKSYDDSIVIAVLKQGKKGLLGHMPSFNERLNETQEKALASYIRSLGDK"
BASE COUNT          704 a          327 c          213 g          828 t
ORIGIN      
        1 ttgagttaaa aattttgtaa aactagaact aattcctagt tttttaatgt caatatcttt
       61 tattgcttca cttaaaagaa gtactctttg ctcttcatca caataaattt tattttcaag
      121 atatatagat ttttttaaaa aatcatcaat tgtaaggcaa gttggcaaaa gagtatttag
      181 agattttttc ttctctaaat agtttctaat tgccctttgc gtaggaaaaa ttataagttt
      241 ttttttactt agcatttgtt tttataaaga taaatcctgt ctcatcttct actttaaaac
      301 ttcctgttat aatccagttt gtctcttcat ttaaactaaa attagtagta taaatatttt
      361 tatcttttgt aaagttttca tcttttagat tcaaattact atcatctgac atagtttttg
      421 ttataagtaa atcaatattt ataggaacta tctcttttgt tattttattt aaaactgaaa
      481 tttttaagct attttctcca acttttaaaa catctttatg gtttgaatac ttttcaataa
      541 ctctttgacc atattttata tcttctgttg taagtgggaa aatttttcca tttaaatcta
      601 attctaaact atatttttcc aaaaaattta tatttgatgt cattatattg ttataatttt
      661 catctacata ttggtatttt tgcataaaac ttctatcttc cacaacagga acttgtgtgg
      721 ctttataaat tgtccaaata atcatataaa cagtaaaagc aaaaattgct ataaaatata
      781 atggccaata atttcttttc ttcatactat tttctctttc gtaaaattgc ataaatatat
      841 gctaaaagag cagacaataa tacaaaatat ataaatactc tccagatcgt actagcaact
      901 cttccttgat taccaatatt actctcaagt tcaatatttt ttgaatctgc taatttatca
      961 gctactgcag cataaccatt taatacagaa gcacttactt tagaagcaac tgtattttta
     1021 tcttttgatg ccaacaaagg cacaacataa tcatcaagaa tttcattttt atctattatt
     1081 ttttctaaag tgtcactgta gtataaattc acatgagtat cttcaagtga aattgttatt
     1141 atcacataag gggtttcaag attttttaaa atatctaatt cataattttt tatatagtct
     1201 attttatctt ttgtagatat atctttatct aaattaaaac tagaagttga ataaacataa
     1261 atatttacac ctgttttagt cttaacttca ttaccaatct caacaatctt atcttttgct
     1321 ctattatcta ttaaatcatc ttttaatata aaattttgtg caaaaccgtt tgtatataaa
     1381 aaaagtagaa gtgaaaaaat cacccctact tttaaagaat taaaattttt catttaacca
     1441 acaaatactt ttgcttggtc aaaaaatgca taataaatcg tcataagagc cgaaaccaaa
     1501 agcaaaatag taattaatct gtccatactt gactccttat ttaccattac caattaattg
     1561 gtaatgttta tagttatcag gactattggc ttttaaagca tttaagtctt gattaatttg
     1621 gtaagcatta gaagcttctc tttgctgaac taaaacacca ttaaaaacta atactgcaag
     1681 aattgtttaa agtaaaacaa cagctattaa cattcctgtt atgccgtgta atttaaaaat
     1741 tcctctttca ttttcattca tttgtgtatt atttgccatt acttatctcc taaacttcta
     1801 atatatgaag ctaaagcttt ctcttgagtc tcatttaatc tttcgttaaa gcttggcata
     1861 tgacctaaaa gacctttttt accttgtttt aaaacagcta ttacaataga atcatcataa
     1921 gatttaatat ttggacctac cattggcatt ccttctccat tatcaccatg acaagttgca
     1981 caagttgcat aagcagctgg ttgctctcct ttaaatccat ttgctacata tgcagaaact
     2041 tctttaatat cagcatcttc gctaagcatc at
//
LOCUS       DAOQAN010000192         2070 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_79977_length_2070_cov_2.383623, whole genome shotgun sequence.
ACCESSION   DAOQAN010000192 DAOQAN010000000
VERSION     DAOQAN010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79977_length_2070_cov_2.383623"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            14..589
                     /locus_tag="PKO10_03785"
     CDS             14..589
                     /locus_tag="PKO10_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HNW66303.1"
                     /translation="MLKCTSCDKLSLQIICKTCQTTLLAPNFYKKELEKDFFIYSFYD
                     YKDLEDLIQSKYHFYGDRVFNILGKLSFKKFADNFEFTHPILALPIDDHTRHDFSQTA
                     ILAKHLKSAFIKPVFNTLKASNIVKYAGKNLEFRQKNPRKFIYSGVKNCDVILVDDVI
                     TTGTTILEAKKALKKEGVNVLFALTLCSFLN"
     gene            624..1229
                     /gene="hisH"
                     /locus_tag="PKO10_03790"
     CDS             624..1229
                     /gene="hisH"
                     /locus_tag="PKO10_03790"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012083715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HNW66304.1"
                     /translation="MLGIIDYNMGNLASVYNACSKFTKDLKIVKNADDLKNLDKIILP
                     GVGAYKDAMQHLEDSGLKDAILDFANSKKPLLGICLGMQLLFESSEEFGYTKGLGLIE
                     GKVVKFDKSKMSSDLKIPHMGWNKTVNKDNPLFKNLQNPYLYFVHSYHVVTDDKYTIA
                     TTNYGYDFVSAVNKDNIFGFQPHPEKSHNNGLKILENFINL"
     gene            1247..1954
                     /gene="hisA"
                     /locus_tag="PKO10_03795"
     CDS             1247..1954
                     /gene="hisA"
                     /locus_tag="PKO10_03795"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014473329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HNW66305.1"
                     /translation="MDILPAIDLKDGKAVRLSKGLMDSAKIYSDEPWQVAKRFEELGS
                     KWVHIVDLNGAFEGKPANLEQIKKIRENCNLKIELGGGIRDEKTIQMYLELGVDRLIL
                     GSIAVKDPQFVKDMAKKYPIAVGIDAINGMVAVEGWAEVSTMKATDLAQEFANAGVEA
                     IICTDISKDGMLCGVNVEFTEDIALASNVYTIASGGVKDIEDIKNCKANGNIGGVIVG
                     KAFYEGTLDLEEAFKIL"
BASE COUNT          806 a          232 c          359 g          673 t
ORIGIN      
        1 tctaaaaggt tttattttga agtgtacaag ttgtgacaag ttatctttac aaattatttg
       61 taaaacttgt caaactacac ttcttgctcc aaacttttat aaaaaagagc tagagaaaga
      121 tttttttatc tactcttttt atgattataa agatttagaa gatttaatcc aaagcaaata
      181 tcacttctat ggagatagag tttttaatat tttaggaaaa cttagtttta agaagtttgc
      241 agacaatttt gaatttaccc accctatttt ggctcttcca atagatgacc atacaagaca
      301 cgatttttct caaactgcta ttttggcaaa gcatttaaaa agtgccttta taaaacctgt
      361 tttcaatacc ttaaaagctt caaatattgt caaatatgct ggaaaaaatc tagaatttag
      421 acaaaaaaat ccaagaaaat ttatctatag tggagtaaaa aattgtgatg taattttagt
      481 tgatgatgta attactacag gaacaactat tttagaagct aaaaaagctt taaaaaaaga
      541 gggtgtaaat gttttgtttg ctcttactct atgctcattt ttaaattaat aaaagatata
      601 atctatgctt taaaaggatt tagatgctag gaataataga ctacaatatg ggaaatctgg
      661 caagtgttta taacgcttgt tcaaaattta caaaagattt aaaaattgta aaaaatgcag
      721 atgatttaaa aaatttagac aaaataatac taccaggagt tggagcatat aaagatgcta
      781 tgcaacattt agaagatagt ggcttaaaag atgctatttt agattttgca aatagtaaaa
      841 aaccacttct tggaatttgc cttggaatgc aacttttatt tgaaagttct gaagagtttg
      901 gatatacaaa aggtttagga ttaattgaag gaaaagttgt gaaatttgat aaatcaaaaa
      961 tgagtagtga tttaaaaatt ccgcatatgg gctggaataa aacagtaaat aaagataatc
     1021 cactttttaa aaatctacaa aacccatatt tatactttgt acactcttat catgttgtta
     1081 cagatgataa atatacaata gcaacaacaa actatggata tgattttgta agtgctgtaa
     1141 acaaagataa tatttttgga tttcaacctc atcctgaaaa gtctcataat aacggactta
     1201 aaatattaga aaactttata aatttataaa tattaaaagg aaaattatgg atatattacc
     1261 agcgattgat ttaaaagatg gaaaggctgt aagacttagc aaagggctta tggatagtgc
     1321 aaaaatctat tcagatgaac catggcaagt ggcaaaaaga tttgaagagt taggaagtaa
     1381 gtgggttcat attgttgatt taaatggagc atttgaagga aaaccagcca acttagagca
     1441 gattaaaaaa attagagaga actgtaattt aaaaattgag cttggtggcg gaataagaga
     1501 tgaaaaaact attcagatgt atttagaact tggagttgat agattaattc ttggctcaat
     1561 cgctgttaaa gatcctcaat ttgtaaaaga tatggcaaaa aaatatccaa ttgctgttgg
     1621 aattgatgca ataaatggaa tggttgcagt tgagggttgg gcagaagttt caacaatgaa
     1681 agctacagac ctagcacaag agtttgcaaa tgctggagtt gaagcaatta tttgtacaga
     1741 tataagcaaa gatggtatgc tttgtggagt taatgttgaa tttacagaag atattgcact
     1801 tgctagcaat gtttacacaa ttgcaagtgg tggagttaaa gatattgaag atatcaaaaa
     1861 ctgtaaagca aatggaaata ttggtggagt aattgttgga aaagcatttt atgaaggtac
     1921 tttagattta gaagaggctt ttaaaatatt gtaattcaat aaaatatttg taatttttta
     1981 taaaatttct aaaaagggaa aacaaatgaa gtcaaatacg aaaataacac ttatcatttt
     2041 ttcaatggtt ctacttttaa cttcaattat
//
LOCUS       DAOQAN010000193         2067 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_80149_length_2067_cov_3.199304, whole genome shotgun sequence.
ACCESSION   DAOQAN010000193 DAOQAN010000000
VERSION     DAOQAN010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80149_length_2067_cov_3.199304"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..154)
                     /locus_tag="PKO10_03800"
     CDS             complement(<1..154)
                     /locus_tag="PKO10_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020246829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HNW66306.1"
                     /translation="MFDEIEFERMKRLPNYVFAQVNAIKMEARRAGVDVIDFSMGNPD
                     GETPKHI"
     gene            complement(159..965)
                     /locus_tag="PKO10_03805"
     CDS             complement(159..965)
                     /locus_tag="PKO10_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66307.1"
                     /translation="MKKLLLIIFLCVSLNANINQAVLGIIGSSDFNTHRNLINTIFKN
                     QSYFYTNGSLDYAKISQTLQNNNLLKLSLGSTQTIEATFIFNSDPKKSFKNINDILKA
                     IGIQNFVTINQSVSENQLKWSIKVQTAAAINPLRLSQELQNTNCRVVDIKKEGNNKWS
                     YYIDSKKSSIYKAEDLVTKASVSLKKPIKPYILEIANTDAIKIDSNIGNNWYPNIIFY
                     DDSFNVIDVFESESLHKNLRVDIPTNTRFIKIDDFYALTNIKNGLNITKE"
     gene            complement(978..1667)
                     /gene="rlmB"
                     /locus_tag="PKO10_03810"
     CDS             complement(978..1667)
                     /gene="rlmB"
                     /locus_tag="PKO10_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HNW66308.1"
                     /translation="MIVYGKQIVLYVLEKHQDLIEEIFLSKEIDSKLFSRFAKLNKKI
                     HKVDNQKAQALAKGGNHQGLILKLSDYHYTPLKDIKNMNFIVCLDGLTDVGNIGAIAR
                     TAYSLGVDGMIAADIKTISNSGTIRTSAGALLDLPFAIHPRSVDLASELIDAGFTLIG
                     ATMDGVDLKKYGKIEKTDKVAIILGNEGAGISPKVAKKLDLKVSIKMEHEFDSLNVSS
                     AAAILIYNLKR"
     gene            complement(1683..>2067)
                     /locus_tag="PKO10_03815"
     CDS             complement(1683..>2067)
                     /locus_tag="PKO10_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014472883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rRNA (cytidine-2'-O-)-methyltransferase"
                     /protein_id="HNW66309.1"
                     /translation="ASKLEEVLNSSRVGILYESPHRLLKLLEELNKKEPDRTIFLVKE
                     ISKFYQKTYKTSEKKLYEELKSIEIKGEWVVVVEPKEKFGFNLELDDILPLDIAPKIK
                     AKLIAKLTGASVKEIYQELLDKIQN"
BASE COUNT          647 a          335 c          225 g          860 t
ORIGIN      
        1 ctatatgttt tggagtctct ccatctggat ttcccataga aaagtcaatt acatcaactc
       61 cagctcttct tgcttccatt ttaatagcat taacttgtgc aaacacatag tttggaagtc
      121 ttttcattct ttcaaattca atttcgtcaa acattttatt actccttagt aatatttaaa
      181 ccatttttta tatttgttaa agcataaaaa tcatcaattt ttataaatct tgtatttgtt
      241 ggaatatcaa ctctaagatt tttatgcaaa ctctcactct caaaaacatc aattacatta
      301 aaactatcat cataaaaaat aatatttggg taccagttat ttccaatatt tgaatcaatt
      361 tttattgcat ctgtatttgc aatctccaaa atatatggct ttataggttt ttttaatgac
      421 acactagcct ttgtaaccaa atcttcagct ttataaatag aagatttttt tgagtcaata
      481 taataactcc atttattgtt accctctttt tttatatcaa caaccctaca atttgtattt
      541 tgtagctctt gagataatct aagagggttt atggcggctg ctgtttgtac ttttatactc
      601 cattttagct gattttctga gacactttga tttattgtta caaaattttg aatacctatt
      661 gcttttaaaa tatcatttat atttttaaaa gatttttttg ggtctgaatt aaatataaaa
      721 gttgcttcaa tagtttgagt agaacctaaa gaaagtttta aaagattgtt gttttgtagg
      781 gtttgagaaa tttttgcata atcaagacta ccatttgtat aaaaatagct ttgattttta
      841 aaaatagtat taatcaaatt tctatgagta ttgaaatcac tacttcctat tattcctaaa
      901 acagcttgat taatatttgc attcaagctt acgcataaaa atattataag tagtagtttt
      961 ttcatatatt ttccttttta tctttttaaa ttgtatatta aaatagcagc tgctgaactt
     1021 acatttaaag agtcaaattc atgttccatt tttattgaaa cttttaagtc taattttttt
     1081 gcaacttttg gactaattcc agcaccttca tttcctaaaa ttattgcaac cttatctgtt
     1141 ttttctattt ttccatattt ttttagatca actccatcca ttgttgcacc tattaatgtg
     1201 aaaccagcat ctataagctc acttgctaaa tctacacttc ttggatgaat tgcaaaaggt
     1261 aaatcaagca aagctccagc acttgttctt atagttccag agttgcttat agtttttata
     1321 tcagctgcaa tcattccatc aactcctaaa gagtaagcag ttcttgcaat tgctccaata
     1381 tttcctacat ctgtaagacc atctaaacaa actataaaat tcatattttt tatatctttt
     1441 agaggtgtat aatgataatc actaagtttc aaaataagcc cttggtgatt tccacctttt
     1501 gctaaagctt gagctttttg attatcaact ttgtgaattt ttttatttag ttttgcaaat
     1561 cttgaaaaaa gtttactatc aatctctttt gataaaaaaa tctcttctat taaatcttgg
     1621 tgtttttcaa gaacatataa aactatctgt tttccgtata ctatcatttt ttctcattta
     1681 aattaatttt ggattttatc caataattct tgataaatct cttttacact agcacctgta
     1741 agctttgcta ttaactttgc ttttattttt ggtgcaatat caagtggtaa aatatcatct
     1801 aattcaagat taaaaccaaa tttctctttt ggttcaacta ctacaaccca ttcacctttt
     1861 atttcaatac tctttaactc ttcatatagt tttttttcac tagttttata tgttttttga
     1921 taaaacttgc ttatctcttt tactaaaaat attgttctat ctggctcttt tttatttaac
     1981 tcttcaagaa gttttaaaag ccgatgaggt gattcatata aaattccaac tctagaacta
     2041 tttaaaacct cttcaagttt tgaagca
//
LOCUS       DAOQAN010000194         2057 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_80723_length_2057_cov_3.208791, whole genome shotgun sequence.
ACCESSION   DAOQAN010000194 DAOQAN010000000
VERSION     DAOQAN010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80723_length_2057_cov_3.208791"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            256..450
                     /locus_tag="PKO10_03820"
     CDS             256..450
                     /locus_tag="PKO10_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013460684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66310.1"
                     /translation="MNSFVINIKDESLKDKVLWLLEHFKNDGLEIVSKEDLDDLKLLK
                     ASREDETIDFEEYLKNENCN"
     gene            434..679
                     /locus_tag="PKO10_03825"
     CDS             434..679
                     /locus_tag="PKO10_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013460683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNW66311.1"
                     /translation="MKIVIRSSAIKDLKSISEPFKTKIEKKILELKKFPNLTNIKKLT
                     NFEPAYRYRIGDYRVLFDIEDDNLIIGRVLHRKESYL"
     gene            681..>2057
                     /locus_tag="PKO10_03830"
     CDS             681..>2057
                     /locus_tag="PKO10_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNW66312.1"
                     /translation="MKENWQEQLKDLLNCDLKTLYPLARSMNRKLEFYVGPTNSGKTY
                     NAMKKLKEANSGLYLAPLRLLALEGYEDLKESNIDASLITGEEQILNEDAAHICSTIE
                     MLDFDLEVDVAIIDEVQMLEDIDRGWAWVNAIIGVPAKKVIMTGSVNALEAVKKIVQY
                     LDEELEVVRHKRKNPLLVLQKWTPLEKLENGTALIAFSRAEVLKLKQKLQKKYSVSVI
                     YGNLSPEVRRDEARRFRDGQTQILIATDAISMGLNLPIKTILFTTDTKFDGVSKRKIS
                     VNEIVQIAGRAGRFGLFEAGYLGATRRDVLEYIKDEFEAPIKTIKPPFKVKINNSQLE
                     NLSMHLKTKSLAKVLNFFALNMKFAGPFEAANLSSMLEASRIVDSKDGLSLEEKYLLA
                     QAPITTKSTIIVQAFNSYIASVIKKRPNHYKPSITLPKKAITQKDLLLVEDEVKKISL
                     YLWLSYKLP"
BASE COUNT          812 a          224 c          353 g          668 t
ORIGIN      
        1 gctctattgc gaaagtgttt tggagtactt tttataatat caaaatcgca aattgtaaga
       61 tttgaaaatc tattcttttc tctttggatt ttatagttta gttgttcatc ataattttta
      121 tcataaagtg tacaagaagc acaagagcca aaatatttac agttcataag attttatcct
      181 ttttttaaag ttcgtattat atgtaaatat tggtaaaagt agatataatt taagaatttt
      241 aaaaaaggag actttatgaa ctcatttgta ataaatataa aagatgaaag tttaaaagat
      301 aaagttttat ggcttttaga acattttaaa aatgatggat tagagatagt ttctaaagag
      361 gatttagatg atttaaaact tttaaaagct tcaagagaag atgaaacaat agattttgaa
      421 gagtatttaa aaaatgaaaa ttgtaattag aagtagcgct ataaaagatt taaaatctat
      481 tagcgaacct tttaaaacaa aaattgagaa aaaaatatta gagcttaaaa aatttccaaa
      541 tcttacaaat attaaaaaat taacaaattt tgaaccagca tatagatata gaatcgggga
      601 ttatagagtt ctttttgata ttgaagatga taatttaata attggtagag ttttacacag
      661 aaaagagagt tatttataat atgaaagaaa attggcaaga gcaactaaaa gatttattaa
      721 attgtgattt aaaaacatta tatccactag caagaagtat gaatcgaaaa ttagaatttt
      781 atgttggccc aacaaatagt ggaaaaacat ataatgcaat gaaaaaatta aaagaggcaa
      841 atagtggatt atatcttgca cctttgagac ttttggcact tgaagggtat gaagatttaa
      901 aagagtcaaa tattgatgct tcattaatca caggtgaaga gcagatttta aatgaagatg
      961 cagctcatat ttgctcaact attgagatgt tggattttga ccttgaagtt gatgttgcca
     1021 ttattgatga agttcaaatg cttgaagata ttgatagagg ttgggcttgg gtaaatgcta
     1081 ttattggagt tcctgcaaaa aaagttatta tgactggaag tgtaaatgct cttgaagctg
     1141 taaaaaagat agttcaatat ttagatgaag agttagaagt tgtaagacat aaaagaaaaa
     1201 atcctttatt ggttttgcaa aagtggacac cacttgaaaa acttgaaaat ggaacagctt
     1261 taatagcttt ttcaagagct gaagttttaa aacttaaaca aaaactgcaa aaaaaatatt
     1321 ccgtatctgt aatttatgga aatctttcac ctgaagtaag acgagatgaa gccagaagat
     1381 ttagagatgg gcaaactcaa attttaattg caacagatgc tataagtatg gggttaaatc
     1441 ttccaattaa aacaatactt tttacaactg atacaaaatt tgatggagtt agtaaaagaa
     1501 aaataagtgt aaatgagata gttcaaatag caggtcgtgc tggaagattt ggactttttg
     1561 aagctggata tttgggtgct acacgaagag atgttttaga gtatatcaaa gatgagtttg
     1621 aagcaccaat aaaaactata aaacctccat ttaaagtaaa gataaataac tcacaacttg
     1681 aaaatttatc tatgcattta aagacaaaat ccttagcaaa agttttaaac ttttttgctt
     1741 taaatatgaa atttgctggt ccttttgaag ctgcaaatct gtcaagtatg cttgaagctt
     1801 caagaattgt tgatagcaaa gatggtttga gtttagaaga gaaatatctt ttggcacaag
     1861 ctccaattac tacaaaatcg actattatag ttcaggcatt taactcatat attgcaagtg
     1921 tgattaaaaa aagaccaaat cactataaac catcaattac tttaccaaaa aaggcgatta
     1981 ctcaaaaaga tttgcttttg gttgaagatg aggtaaaaaa aatatcttta tatctttggc
     2041 tttcgtataa actgcct
//
LOCUS       DAOQAN010000195         2056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_80793_length_2056_cov_2.959020, whole genome shotgun sequence.
ACCESSION   DAOQAN010000195 DAOQAN010000000
VERSION     DAOQAN010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80793_length_2056_cov_2.959020"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            250..1608
                     /locus_tag="PKO10_03835"
     CDS             250..1608
                     /locus_tag="PKO10_03835"
                     /inference="COORDINATES: protein motif:HMM:NF016571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNW66313.1"
                     /translation="MGLDNFIETAMNKVLKNPIVEVLRDINFSSILKQSNFKKRDIGV
                     KPYMIILQFLYMFIINKKISTFMKYSSDSYKKDVYYRLLKNGRYNWRKLLLLTSVKLL
                     SKLVYFQNSTDTKVLIIDDTVEIKRGKYIEGSCKNLWSNKEKRVVKGLNIVSLNYSDS
                     YTDLMLDFSLNYNKNQIVDFTDTTIHHSSNAYKRRIEGINGKNLQAINMINRALNAGI
                     YADYLLVDSWYAKPNFINEVKQNGLDVIARVSKSNKIWQFIGNSKTLESLYNQAKHHK
                     CSKLGNYNSIKYSYVSAIVTHKTLGKIKIVFIKTKDNLIPIISTNIILSDIEIINTYK
                     KRWNIEQGYKDLREYFQFGKEENRIYEALIARITLSFLAYNLTSYINRINNEPQTLGN
                     LFRDLECQLETLAISMEIFLKILEQLIQSAQIVKRNKDLEQIIHMLRVYTKKQLGFMC
                     ES"
     gene            complement(1655..>2056)
                     /locus_tag="PKO10_03840"
     CDS             complement(1655..>2056)
                     /locus_tag="PKO10_03840"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNW66314.1"
                     /translation="YCLSSIKLQLCDYLIKPINLDQVIISLEKIAKILKKDLLPLIIE
                     INEGVFYKPVERIICVDNHISKLTTQESDLLNYLILNRGNIITKQMVEDKIYIFKEMG
                     ESALKNIIYKLRKKLVKNVIITVDRIGYKIE"
BASE COUNT          826 a          249 c          260 g          721 t
ORIGIN      
        1 aaagtgaata ttcgaatgat ggagtaagac tatttcaaat tgcaaatgtt acttttggaa
       61 aaattatttg ggataagacg gcttatttac cttatgaata tttaaaaaat gagaaatatg
      121 aaaaattaac tcaactttcg cacatagaaa atcataaatt tcctatataa aaaagcttcc
      181 ttaaatccca taaataggca cttttagata taataacatt gaccaaaaca ccattataga
      241 aggcttttta tgggacttga caattttatc gaaacagcta tgaataaagt attaaaaaat
      301 ccaatagttg aagttttaag ggatataaat tttagtagta ttcttaagca aagtaatttt
      361 aaaaagcgtg atattggtgt aaaaccatat atgataatct tacaattttt atatatgttc
      421 attatcaata aaaaaatatc aacttttatg aaatacagta gcgatagtta taaaaaagat
      481 gtctattata gacttttaaa aaatggtcgt tataattgga gaaaactatt acttttaaca
      541 tctgtaaaac tcttatctaa acttgtatat tttcaaaatt ctacagatac aaaagttctt
      601 attatcgatg atacagttga aatcaaacga ggtaagtata tagagggtag ttgtaaaaat
      661 ctttggagca ataaagaaaa aagagttgtt aagggattga atattgtgtc actcaattac
      721 agtgattcat atactgattt aatgctagat ttttcactaa attataataa aaatcagatt
      781 gtagatttta cagatacaac aattcatcat tcaagtaatg cttacaaacg aaggattgaa
      841 gggattaatg gtaaaaactt acaagctatc aatatgataa atagagcttt gaatgcagga
      901 atatatgctg attatcttct tgtagatagt tggtatgcaa aaccaaattt tatcaatgaa
      961 gtcaaacaaa atggtcttga tgtgattgca agagtttcaa aatcaaacaa aatttggcaa
     1021 tttataggga attcaaaaac ccttgaatca ctttataatc aagctaaaca tcataaatgt
     1081 tcaaaactag gtaactataa ttctatcaaa tatagctatg tttctgctat tgttactcat
     1141 aaaacacttg gaaaaatcaa aatagttttt ataaaaacaa aagataatct tataccaata
     1201 atttctacaa atataatttt atcagatata gaaattatca atacatacaa aaaacgatgg
     1261 aatatagaac aaggatataa agatttgaga gaatattttc aatttggtaa agaggagaat
     1321 cgaatttatg aagctttaat agctagaata actctatcat tccttgctta taatctaaca
     1381 agctacatta accgaatcaa taatgaacca caaactcttg gtaatctttt tagagatttg
     1441 gagtgccaac ttgaaacttt agctatatca atggaaatat tcctaaaaat attagaacaa
     1501 ttgatacaat cagctcaaat tgtcaagaga aataaagatt tagaacagat tatccatatg
     1561 ctaagggttt acaccaaaaa acagttaggt tttatgtgcg aaagttgagt tattaaatat
     1621 atctttttca ttatatttta aaatagaata tctattattc tattttgtaa cctattctat
     1681 caactgtaat aattacattc ttaactaatt tttttctaag tttataaata atatttttta
     1741 aagcagattc tcccatttct ttaaaaatat aaattttatc ttcaaccatc tgcttagtaa
     1801 taatattccc tctattaaga attaggtaat ttaataaatc actttcttgt gttgttaatt
     1861 tactaatatg attatctaca caaataattc tttctacagg tttataaaaa acaccctcat
     1921 ttatttcaat aattaatggt aataaatctt tttttaatat ttttgcaatt ttttcaagag
     1981 atataattac ttgatcaaga ttaattggtt taattaaata gtcacataat tgtaatttga
     2041 tagaagataa gcaata
//
LOCUS       DAOQAN010000196         2055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_80838_length_2055_cov_3.399000, whole genome shotgun sequence.
ACCESSION   DAOQAN010000196 DAOQAN010000000
VERSION     DAOQAN010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80838_length_2055_cov_3.399000"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            141..1016
                     /gene="mqnP"
                     /locus_tag="PKO10_03845"
     CDS             141..1016
                     /gene="mqnP"
                     /locus_tag="PKO10_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis prenyltransferase MqnP"
                     /protein_id="HNW66315.1"
                     /translation="MEKLIKKIKDFSELVMFQHSVFALPFIFIAMVVSSSQINGTPWF
                     GFKLLVLGTLCAVFARNFAMGFNRFMDRDIDALNPRTKNRPNVDGRVSPKAMFIFFLA
                     NAFGFILVAYFVNDLALILSLPILVIIGSYSYFKRFSYLAHIILGISLALAPIAGVVA
                     VSETIPLWVIFLSIGVMFWVAGFDLLYSLQDIDVDKKLGLHSIPSKFGAKKTMLFSKV
                     FHFLTIVFWLLFVIYASGSYFAYLAVVISALMLSYEHYLVNKDFRKIDRAFFTVNGYL
                     GIVFFFLVVLDNIFF"
     gene            1064..1140
                     /locus_tag="PKO10_03850"
     tRNA            1064..1140
                     /locus_tag="PKO10_03850"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1098..1100,aa:Arg,seq:tcg)
     gene            1247..>2055
                     /locus_tag="PKO10_03855"
     CDS             1247..>2055
                     /locus_tag="PKO10_03855"
                     /inference="COORDINATES: protein motif:HMM:NF016220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HNW66316.1"
                     /translation="MSMFQKSIINSVKQDESKVALRWASFQKFLEKVEYIKTVKEEKY
                     QDGFLVDIFENCLGYTLDMTNPKSFNLEREKKNETDGKKADGVIYVDDKVVGVIELKG
                     QDTKNLDKIETQAFNYHASHSNSKYIIISNFDELRFYVDKKTAYEKFNLFTLNYEEFK
                     KLHLLISYESIKDDVPLKLKEKTNSFEQDISKKLYKDFSNFRTALFENIVKNNLGNET
                     LVSSVLNGEALNQQTLLRLTQKLCDRIIFILFAEDRDLLRPNMIKEIRKRY"
BASE COUNT          719 a          265 c          327 g          744 t
ORIGIN      
        1 gctacaagct gtaaaaatta tagaaaaaag gcttaaaagt attagaaaca tatatttttt
       61 cataattaat tcccgattaa aatttgattg attataacca aaaagcatta atttactcat
      121 tatttgataa aatccagccc atggaaaaat taataaaaaa aataaaagat tttagcgaat
      181 tagtgatgtt tcagcactct gtttttgctt taccatttat atttatagcg atggttgtat
      241 cttcttcaca aataaatgga actccttggt ttggatttaa acttctggtt ttaggaacac
      301 tttgtgcagt atttgcacga aactttgcaa tgggtttcaa ccgttttatg gatagagata
      361 tagatgcttt aaatccacga acaaaaaata gaccaaatgt agatggaaga gtatctccta
      421 aagctatgtt tatattcttt ttggcaaatg cttttggatt tattttagta gcttattttg
      481 taaatgattt ggctttgatt ttatcacttc ctattttggt aattattggt tcatactcat
      541 actttaagag attttcatat ttagcacata ttattttggg aatctctttg gctcttgctc
      601 ctattgctgg ggttgttgct gttagtgaaa ctattccttt atgggtaata tttttaagta
      661 ttggagttat gttttgggtt gctgggtttg atttacttta ctcactacaa gatattgatg
      721 tagataaaaa actaggtctt cactcaattc cttcaaaatt tggtgcaaaa aagacaatgc
      781 tattttcaaa agtttttcac tttttaacta ttgttttttg gcttctgttt gtgatttatg
      841 cgtctggttc atattttgca tatttggcag ttgttataag tgctttgatg ctaagttatg
      901 agcactattt agtaaataaa gattttagaa aaatagatag agcatttttt accgtaaatg
      961 gatatttagg aatagttttc ttctttttag tagttttaga taatatcttc ttctaaaatt
     1021 tttaagcaaa attaggataa acttcaatcc taatttttca agtgcgtctg tagttcaact
     1081 ggatagaata ctcggtttcg acccgagggg ttgtgggttc gactcctacc aggcgtacca
     1141 ctttaaagtt tttataaaat caatttttaa tatttctatt tttatcttct ttttcaaaaa
     1201 aaatggtaac atttaagctt aataaaatca cacaaggttt ctttttatgt caatgtttca
     1261 aaagtcaata ataaatagtg taaaacaaga tgaaagtaaa gttgcacttc gttgggcttc
     1321 atttcaaaag tttttagaaa aagttgaata tataaaaaca gtaaaagagg agaagtatca
     1381 agatggtttt ttggtagata tttttgaaaa ctgcttaggc tatactcttg atatgacaaa
     1441 tccaaaatct tttaatctag aacgagaaaa gaaaaacgaa acagatggca aaaaagcaga
     1501 tggagtgatt tatgtagatg ataaagttgt aggagtaatt gagcttaaag gacaagatac
     1561 aaaaaatctt gacaaaatag agactcaagc atttaactat cacgcttcgc actcaaactc
     1621 aaaatatata ataatctcaa atttcgatga gctaagattt tatgtagata aaaagacagc
     1681 ttatgagaag tttaatcttt ttactctaaa ttatgaagag tttaaaaagc ttcatctttt
     1741 aatctcttat gagagtataa aagatgatgt acctttaaaa ctaaaagaga aaacaaacag
     1801 ttttgagcaa gatatttcaa aaaaactata taaagatttc tcaaacttta gaactgctct
     1861 ttttgaaaat attgtaaaaa acaatcttgg aaatgagact ttagtctcat ctgtattaaa
     1921 tggtgaggct ttaaaccaac aaacacttct tcgcttaaca caaaaactat gcgaccgtat
     1981 tatttttata ctatttgcag aagatagaga tttgcttcga ccaaacatga taaaagaaat
     2041 aagaaaaagg tatca
//
LOCUS       DAOQAN010000197         2054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_80923_length_2054_cov_2.534267, whole genome shotgun sequence.
ACCESSION   DAOQAN010000197 DAOQAN010000000
VERSION     DAOQAN010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80923_length_2054_cov_2.534267"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /locus_tag="PKO10_03860"
     CDS             <1..706
                     /locus_tag="PKO10_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HNW66317.1"
                     /translation="AISCGKVLKPLVVNEENFKGVNSKVELADAEVIHQNRIKKEFMK
                     AGVILRLPDTIYIEEGVTIEGESIIENGVSLLGNSKIINSHIKTNSVVEDSIIKDSDV
                     GPMARIRPDSELNKTHIGNFVETKKAKLNGVKAGHLSYLGDCTIDEGTNIGCGTITCN
                     YDGVNKHQTIIGKNVFVGSDTQFVAPVKIEDDVLIAAGSTVTGDVKKGELYLTRAKAK
                     TIDGYFYKHFSSKKKS"
     gene            703..1917
                     /gene="coaBC"
                     /locus_tag="PKO10_03865"
     CDS             703..1917
                     /gene="coaBC"
                     /locus_tag="PKO10_03865"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014475378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HNW66318.1"
                     /translation="MILKDKNILLAVTGSIAIYKSLELIRLFIKAGANVKVILSDGAK
                     KFINPITFEAISQNRVLDESSESWDKSQNYNHIDITKWADILLIAPATANTINKISCG
                     IADNLLLQTVLACKKKIIIAPAANTNMIENPITTKSVNNLKNLGFEVLQTQTKELACK
                     DVGNGAMLEPIDIFHKTCKELLKTSYWENRRAVLSGGGTLEKIDDVRYISNFSSGKMA
                     SSLATALYYLGADVTLVSSRGFENIPYEIDMRVSHSSQQMFENLKLALDKKLDKKSFL
                     FMVAAVSDYIPKEKFDGKLKKEKLGETWSLELSKNIDILANLDKKDIFSIGFKAEMDK
                     KEAKSNAQNMLKNKNLDAVCLNILDEANSFGSDTNKIELLLKDKSFDFYGNKLNISLE
                     ILNRFEKEFTNE"
     gene            1910..2054
                     /locus_tag="PKO10_03870"
                     /pseudo
     CDS             1910..2054
                     /locus_tag="PKO10_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014475377.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl diphosphate synthase family
                     protein"
BASE COUNT          837 a          212 c          378 g          627 t
ORIGIN      
        1 ggcaatatct tgcggaaaag ttttaaaacc tttagttgta aatgaagaga attttaaagg
       61 agttaattca aaagttgaat tagcagatgc tgaagtaatt catcaaaata gaattaaaaa
      121 agagtttatg aaagctggag taattcttcg attacctgat actatttata tagaagaggg
      181 agttactatt gaaggtgaaa gtatcattga aaatggtgta agtctgcttg gaaactcaaa
      241 aattataaac tcacatataa aaacaaatag tgtagttgaa gactcaatta taaaagatag
      301 tgatgttgga ccaatggcaa gaatacgacc agatagcgaa ttaaataaga cacatattgg
      361 aaactttgta gagacaaaaa aagctaaatt aaatggtgta aaagcagggc atctatcata
      421 tcttggagat tgtacgattg atgagggcac aaatatcggt tgtggaacaa taacttgtaa
      481 ttatgatgga gtaaataaac atcaaacaat tattggaaaa aatgtttttg ttggaagtga
      541 tacgcaattt gttgcacctg taaagataga agatgatgtt ttaatagcag ctggttcaac
      601 agttactggt gatgtgaaaa aaggtgaatt gtatttgaca cgagcaaaag caaaaactat
      661 tgatggttat ttttataaac atttttcatc taagaagaaa tcgtgatttt aaaagataaa
      721 aatatacttt tagcagtaac tggctcaatt gctatttata aaagcttaga gttaataaga
      781 ctttttataa aagctggtgc aaatgtaaaa gtaattttaa gtgatggtgc aaaaaaattt
      841 ataaatccaa ttacttttga agcaatttca caaaacagag ttttagatga gagtagtgaa
      901 agctgggata aaagtcaaaa ctataatcac atagatataa caaaatgggc tgatatttta
      961 ctaatcgctc cagcaactgc aaacactata aataaaatat cttgtggtat tgctgataat
     1021 ttactacttc aaacagtgct tgcttgtaaa aagaaaatta ttattgcacc tgctgcaaat
     1081 acaaatatga ttgaaaatcc tattactact aaaagtgtaa ataacttaaa aaatttagga
     1141 tttgaagttt tacaaactca aacaaaagag ttagcttgta aagatgttgg aaatggtgca
     1201 atgcttgagc caattgatat ttttcataaa acttgcaaag aacttttaaa aacttcatat
     1261 tgggaaaata gaagagctgt tttaagtggt ggtggaactt tggaaaaaat tgatgatgta
     1321 agatatatct caaatttttc aagtgggaaa atggcatcaa gtttagcaac agcactttat
     1381 tatttaggtg cagatgtcac tttagtatca agtagaggtt ttgaaaatat accttatgag
     1441 atagatatga gagtttcaca tagttcgcaa cagatgtttg aaaatttgaa attagcttta
     1501 gataaaaaat tagataaaaa gagtttctta tttatggtag cagcagttag tgattatatt
     1561 ccaaaagaaa aatttgatgg aaaattaaaa aaagagaaac ttggagaaac ttggagttta
     1621 gagcttagta aaaatattga tatattggca aatttagata aaaaagatat tttttctatt
     1681 ggttttaaag cagagatgga taaaaaagag gcaaaatcaa atgcacaaaa tatgctaaaa
     1741 aataaaaatc ttgatgcagt ttgcttaaat attttagatg aagctaatag ttttggaagt
     1801 gatacaaaca aaatagagct tttattaaaa gataaaagtt ttgattttta tggaaataaa
     1861 ctaaatatct ctttagaaat tttaaataga tttgaaaaag agtttacaaa tgagtagttc
     1921 aaatccccca tctcatattg caataataat ggatggaaat ggaagatggg caaaacaaag
     1981 agatcttaaa agaactgcag gtcaaagctg gagcaaaaac tgtaagagag attacagagt
     2041 attgtgcaaa aata
//
LOCUS       DAOQAN010000198         2051 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_81083_length_2051_cov_2.739980, whole genome shotgun sequence.
ACCESSION   DAOQAN010000198 DAOQAN010000000
VERSION     DAOQAN010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81083_length_2051_cov_2.739980"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1189)
                     /locus_tag="PKO10_03875"
     CDS             complement(<1..1189)
                     /locus_tag="PKO10_03875"
                     /inference="COORDINATES: protein motif:HMM:NF033559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HNW66319.1"
                     /translation="MFVRKKINSSGSISVQILEKTNRTNKLIQTVGSSKDEIEIERLY
                     KRAFEIIDQLKQQSRFNFYSNQDESILNFTQQLSNSNIQAVGSQLIFGKLFDSMGFNA
                     IDNKLFKDLVLSRIIYQGSKLKLTEYLKRYENKSISIQRIYRFLDKVHSSYKEQAEDI
                     AFLHTKKVLKNITVVFYDMTTLYFESGDEDDFRKIGFSKDGKFQNPQIMLGLLVGEYG
                     YPIGYELFEGNTFEGHTLIPVLEKFQKRFNLLKPIIIADSGLLSKSNIEQLKAKNYQY
                     ILGARIRTSDSRLSQEILSLNLNEDNSIGKIKTIDNDNLIVSYTPKRAKKDEYNRTKG
                     LKRLEKKVKSGKLTKDQINSRGYNKYLHLKNEISVEIDYEKFYQDSYWDGLKGYITNT
                     NLSN"
     gene            complement(1411..>2051)
                     /locus_tag="PKO10_03880"
     CDS             complement(1411..>2051)
                     /locus_tag="PKO10_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014469439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNW66320.1"
                     /translation="LAMVTDMKTAVIYVAIPTLVLNLSVLIIEGNFLQTIKRFYLLAI
                     IAMIGSAIGTQILIYSNSEIFKFLLAISILFYLFSQKISFEMPWVRSKPNLATFIFGL
                     SAGVIGGLTNVMALVLIIYSLESRHSKKEIIQSTNLCFMFGKIIQIAIFIAHGSFTQE
                     ILEISLYNLLIVGICLMIGFNLRKKIASDSYFKFIKAFLFFMAIALILQTIF"
BASE COUNT          725 a          295 c          222 g          809 t
ORIGIN      
        1 catttgaaag atttgtattt gttatatatc cttttaaacc atcccaatat gaatcttgat
       61 aaaatttttc ataatctatt tctacactta tttcattttt aagatgtaga tatttattat
      121 atcctctact atttatttgg tcttttgtga gttttccaga ctttaccttt ttttctaatc
      181 ttttcaaacc ttttgtacgg ttatactcat ctttttttgc tctttttggt gtatacgata
      241 ctattaaatt atcattatct atcgttttaa ttttaccaat agaattatct tcatttaaat
      301 taagcgataa aatttcttga gataatcttg aatcactagt tctaattctt gctcctaaaa
      361 tatattgata atttttagct tttaattgtt caatattact tttagataat aatcctgaat
      421 cagcaattat aattggtttt aaaagattaa atcttttttg aaatttctct agaacaggaa
      481 ttagtgtatg tccctcaaag gtattacctt caaacaattc atatcctatt ggatatccat
      541 attcaccaac taaaagtccc agcattattt gaggattttg aaatttacca tctttactaa
      601 atcctatttt tctaaaatca tcttcatcac cactttcaaa gtacaatgtt gtcatatcat
      661 aaaatacaac agtgatattt tttaatactt tttttgtatg taaaaaagca atatcttcag
      721 cttgttcttt atagctacta tgaactttat ctaagaaacg atatattctt tgaattgaaa
      781 tactcttatt ttcatatcgt ttaagatact cagttagttt taatttactt ccttgataaa
      841 ttattcttga aagaactaaa tctttaaata atttattatc aatagcatta aagcccatag
      901 aatcaaacaa tttcccaaat atcaattgag aacctactgc ttgaatatta ctatttgata
      961 attgctgtgt aaaatttaaa attgattcat cttgattact ataaaaatta aatcttgatt
     1021 gttgttttaa ttgatcaatt atctcaaaag ctctcttata aagtctttca atctctattt
     1081 catcttttga ggaaccaaca gtttggataa gtttatttgt tctattagtt ttttctaaaa
     1141 tttggacact tattgagcca cttgagttta ttttttttct tacaaacatg aacaattata
     1201 acatgggtca cccaaaagtg actcaaaaat agtcttaaag cctttattat agggattctt
     1261 acttttttaa tcaaaaaagc gacgaagtca ggaggaaaat ggtgggcatt aggagcaatt
     1321 tttggtgcta tttatcttat tcctataatt attggtgtat ttatatttat gtaagatatt
     1381 atgaggatgg gttaaaatcc catcttcaat ttaaaaaata gtttgtaaaa tcaaagctat
     1441 tgccataaaa aataaaaaag cttttataaa tttaaagtaa ctatctgaag ctatcttttt
     1501 tcttaaatta aaacctatca tcaaacagat tccaacaatt aaaagattat ataaagatat
     1561 ttctaatatc tcttgagtaa atgaaccatg agctatgaaa atagctattt gaataatttt
     1621 cccaaacata aaacagagat ttgtagattg aattatctct tttttactat gtcttgattc
     1681 taaagaatag ataattaaaa ctaatgccat aacatttgtt aaaccaccaa taactccagc
     1741 acttaaacca aaaataaaag ttgctaaatt tggtttactt cttacccaag gcatttcaaa
     1801 agatattttt tggctaaaaa gataaaaaag aattgaaatt gctaaaagaa atttaaatat
     1861 ttcagaattg ctataaatca aaatttgtgt tcctattgcg cttccaatca ttgcaataat
     1921 agctaaaaga taaaaccttt ttatggtttg taaaaaattt ccttcaataa ttaatacact
     1981 taaatttaat actaaagtag gaatcgcaac atatataaca gctgttttca tatctgtaac
     2041 cattgcaaga a
//
LOCUS       DAOQAN010000199         2031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_82299_length_2031_cov_2.781883, whole genome shotgun sequence.
ACCESSION   DAOQAN010000199 DAOQAN010000000
VERSION     DAOQAN010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82299_length_2031_cov_2.781883"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..106)
                     /locus_tag="PKO10_03885"
     CDS             complement(<1..106)
                     /locus_tag="PKO10_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HNW66321.1"
                     /translation="MSMPSGMELVIIVIIVLILFGGKKIPELAKGLGSG"
     gene            complement(124..354)
                     /gene="tatA"
                     /locus_tag="PKO10_03890"
     CDS             complement(124..354)
                     /gene="tatA"
                     /locus_tag="PKO10_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HNW66322.1"
                     /translation="MHMPSGMQLLVIVLIVLILFGGKKIPELAKGLGSGIKNFKKAVK
                     EDDEEVATASKIEENEKKSDAKSTSSTETKQS"
     gene            complement(420..806)
                     /gene="crcB"
                     /locus_tag="PKO10_03895"
     CDS             complement(420..806)
                     /gene="crcB"
                     /locus_tag="PKO10_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HNW66323.1"
                     /translation="MSLNFQIAIAVGFGGAFGSILRFYAVDYVHKMELGNFPFGILLV
                     NIFGSFLIGILYAYFSTHDVSTILKAFLIAGFLGGLTTFSTFALDSYLLFNSSLNYAI
                     LNILSNLIGSIFFVLIGFKLAIFFIK"
     gene            complement(796..1482)
                     /locus_tag="PKO10_03900"
     CDS             complement(796..1482)
                     /locus_tag="PKO10_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNW66324.1"
                     /translation="MRKIRGLIVFIQFSISVAIVVFFMYLFRNHTHKVIKIWMKIQMF
                     FLGIKLEIEGNLDESCDLILINHQSMLDIIVMEHLHSRNIAWVAKKEITDLFFFGHII
                     KAPRMISIDRENKAGLIHLLSEAKDRLSKGRPIAMFPEGTRSDGTYIGEFKAGAKMLG
                     NKFNLKVQPVVMFNTRNIVNSQKMEAAPGVVKVIYLEPIVASKDTSWYEDCEKNMKEV
                     FEKEYKNYVS"
     gene            complement(1469..>2031)
                     /locus_tag="PKO10_03905"
     CDS             complement(1469..>2031)
                     /locus_tag="PKO10_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014469390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66325.1"
                     /translation="NLSDFSLKNIEEQGVSSIKEWENIENLVYYFVTPIFQKNLIFTY
                     FDTTTNSFKEVKVPLILQNELVSTQTDLNPNDSTFEKYKKIAAIIVFVIFLLIYIWKR
                     WKIVLFFTFISLIVAIIYNLPNQKGVIKTDSYIYILPTKNSTVFFKTQKDERVEALEK
                     RENFVKVLGVDSDFIGWVKEESFEKN"
BASE COUNT          724 a          342 c          213 g          752 t
ORIGIN      
        1 taccacttcc taaaccttta gcaagttctg ggattttttt cccaccaaat aaaatcaaca
       61 caattataac tataattact agttccattc cgcttggcat actcataaaa atcctttata
      121 aaattaagat tgttttgttt ctgttgaaga agttgatttt gcatcacttt tcttctcatt
      181 ctcttctatt tttgaagcag ttgctacttc ttcatcatct tcttttacag cttttttgaa
      241 gtttttaata ccacttccta aacctttagc aagttctggg atttttttcc caccaaataa
      301 aattaacacg attaaaacaa ttactaataa ttgcatacca cttggcatat gcatagctat
      361 tccttttttt gatataagaa atattataac taaaaaagct taaaaatttt taactataat
      421 tactttataa aaaagatagc tagtttaaat ccaattaata caaaaaatat tgacccaata
      481 agatttgata aaatatttaa tatagcataa tttaaagagc tattaaaaag tagatagcta
      541 tctaatgcaa atgtagaaaa tgttgtaaga ccacctaaga atccagctat taaaaatgct
      601 tttaatatag ttgaaacatc atgtgtagag aaataggcat ataaaatacc tattagaaaa
      661 gaaccaaaaa tattaactaa aagtatccca aaaggaaaat ttcctaactc cattttatgg
      721 acataatcta cagcataaaa tcttaaaatt gacccaaatg ctccaccaaa tccaacagca
      781 atagctattt gaaaattaag agacatagtt tttgtactct ttttcaaaaa cttctttcat
      841 atttttttca caatcttcgt accaagatgt atctttactt gcaacaatag gttctaaata
      901 tataacttta acaactccag gagctgcttc cattttttga gagttaacta tatttctagt
      961 gttaaacata acaactggtt gaacttttaa gttgaatttg ttaccaagca tttttgcacc
     1021 agctttaaac tctccaatat aagttccatc acttcttgtt ccttctggaa acattgcaat
     1081 tggacgacct tttgataatc tatcttttgc ttcacttaaa aggtgaataa gtccagcctt
     1141 attctctcta tcaatactta tcattctagg tgcttttata atgtgtccaa aaaagaatag
     1201 atctgttatc tctttttttg ctacccaagc tatatttcta ctatgtagat gttccattac
     1261 aataatatca agcatagatt ggtgatttat taaaattaaa tcacagcttt catctaaatt
     1321 accttcaatt tcaagcttaa ttccaagaaa aaacatctgg attttcatcc atatttttat
     1381 gactttatgt gtgtggtttc taaacagata cataaaaaat acaacaattg caacacttat
     1441 ggaaaactgt ataaaaacta ttaagcccct aatttttctc aaaactctcc tccttaaccc
     1501 agccaataaa atcactatct actcctaaaa cttttacaaa gttttctctt ttttctaaag
     1561 cttcaactct ttcatctttt tgtgttttaa agaatactgt tgagttcttt gttggtaaaa
     1621 tatatatata agagtctgtt tttattacac ctttttgatt tggcaaattg tatataatag
     1681 caacaattaa agatataaaa gtaaaaaata gaacaatttt ccatcttttc caaatataaa
     1741 tcaaaaggaa tatcacaaaa acaataatag cagctatttt tttatacttt tcaaaggtag
     1801 aatcatttgg atttaaatct gtttgtgtac ttactaactc attttgcaag ataagtggta
     1861 cttttacctc tttaaaacta tttgttgtag tatcaaaata tgtaaatatt agatttttct
     1921 gaaatattgg agtaacaaaa tagtaaacta aattttcaat attttcccac tcttttatac
     1981 ttgaaacacc ttgttcttct atatttttca aagaaaaatc actcaaattt g
//
LOCUS       DAOQAN010000200         2031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_82314_length_2031_cov_2.256073, whole genome shotgun sequence.
ACCESSION   DAOQAN010000200 DAOQAN010000000
VERSION     DAOQAN010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82314_length_2031_cov_2.256073"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /gene="mog"
                     /locus_tag="PKO10_03910"
     CDS             complement(<1..521)
                     /gene="mog"
                     /locus_tag="PKO10_03910"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012857107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HNW66326.1"
                     /translation="MIAKIGIITTSDRASAGIYEDLSGKAIIDTLNSYLKSPWQEVYR
                     CISDDRMTIEETLIDLIDNQKCCLVVTTGGTGPSLRDVTPEATEAVCDRMMPGFGELM
                     RSVSLQYVPTAILSRQTAGLRGSSLIVNLPGKPKSIKECLDAVFPAIPYCIDLMEGPY
                     LETNEEIIKAFRP"
     gene            complement(518..1036)
                     /gene="bioV"
                     /locus_tag="PKO10_03915"
     CDS             complement(518..1036)
                     /gene="bioV"
                     /locus_tag="PKO10_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimelyl-ACP methyl ester esterase BioV"
                     /protein_id="HNW66327.1"
                     /translation="MMICNNFYSGFCFKNECEIFKDYLEIGDFIISGFSYGAIKAFKQ
                     ALESKARVDKLQLFSPAFFQTKDEKFKRMQLMFFKKDEDTYIKNFLENLKDKSTKSIE
                     NFFKKGSFKELDELLNFKWSKEDLEELIKKGIKIEVFLGQNDKIIDSLAAKEFFKEFA
                     TVYYFKDKGHLL"
     gene            complement(1020..>2031)
                     /locus_tag="PKO10_03920"
     CDS             complement(1020..>2031)
                     /locus_tag="PKO10_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNW66328.1"
                     /translation="GVGKTLIARAVAGEAQVPFFYQSGASFVHIYVGMGAKKVRELFS
                     SAKINAPSIVFIDEIDAVGKMRSGKSNDERESTLNELLTQMDGFDGESGVIVIAATNK
                     IEVLDDALLRAGRFDRRLYVGLPNMEDRRKILELYLKDVKYEINIQKLSNETSGFSSA
                     ALATLVNEALLNMIKNNNQSLSENDIEIAKNKLEFGKKQLKILDSEQKEILAIYQTCK
                     AYITKSKVNLLEEGVKKINKVFLSFEELQENIKRELSGSVGLELIKNNKFAIGEEDIK
                     RAEDIAVLMVEKYKMAKDSKDIIFDAQESLKLEFSQNIEKINRLKDIMLKNEVITLDD
                     LQ"
BASE COUNT          627 a          393 c          197 g          814 t
ORIGIN      
        1 ttaggtctaa atgcttttat aatctcttca ttagtctcta aatatggacc ttccatcaaa
       61 tcaatacagt atggaattgc tggaaaaact gcatctaaac actcttttat tgattttggt
      121 tttcctggaa gatttactat taaagagctt cctctgagtc ctgctgtttg tcttgaaaga
      181 atagctgttg gaacatattg taaacttaca cttctcataa gctctccaaa tcctggcatc
      241 attctatcac atacagcttc ggttgcttct ggagttacat ctctaagaga aggtcctgtt
      301 cctcctgttg ttacaaccaa acagcacttt tgattatcta ttaaatctat taaagtctct
      361 tcaatagtca ttctatcatc actaatacat ctataaacct cttgccaagg agattttaaa
      421 taactattta aagtatcaat aatagccttt cctgataaat cctcataaat tccagcacta
      481 gctctatcac ttgttgtaat tattccaatt tttgcaatca taataaatgt cctttatctt
      541 taaaatagta aacagttgca aactctttaa aaaactcttt tgcagctaaa ctatcaatta
      601 ttttatcgtt ttgtcccaaa aaaacctcta ttttaatacc tttttttatc aactcttcta
      661 aatcttcttt gctccattta aaatttaaaa gctcatctaa ctctttgaaa cttccttttt
      721 taaagaagtt ttctatactt tttgttgatt tatctttcag attttctaag aaatttttga
      781 tataagtatc ttcatctttt ttaaaaaaca taagttgcat tcttttaaac ttctcatctt
      841 ttgtttgaaa aaatgcaggt gaaaaaagtt gaagtttatc aactcttgct ttactttcta
      901 aagcttgttt aaaagctttt atagctccat atgaaaatcc agagattata aaatccccta
      961 tttctaaata atccttaaaa atttcacact catttttaaa acaaaatcca ctataaaagt
     1021 tattgcaaat catcaagagt tattacctca tttttaagca taatatcttt taatctattt
     1081 atcttttcaa tgttttgtga aaattcaagt tttagactct cttgtgcatc aaaaattata
     1141 tctttgctat cttttgccat tttatatttt tcaaccatta aaactgcaat atcttctgct
     1201 cttttaatat cttcttcacc tattgcaaat ttattatttt ttattaactc aagcccaaca
     1261 cttcctgata attctctttt aatattctct tgcaactctt caaaagataa aaatacttta
     1321 tttatttttt ttacaccctc ttctagtaaa tttacttttg attttgtaat atatgcctta
     1381 caagtttgat aaattgctag aatctctttt tgctcactat caagaatctt gagctgtttt
     1441 tttccaaact ctagcttgtt ttttgcaatt tcaatatcat tttcacttaa tgattgatta
     1501 ttatttttta tcatatttaa aagagcttca tttaccaatg ttgctaatgc agctgagcta
     1561 aaacctgatg tctcatttga aagtttttga atatttatct catatttaac atcttttaaa
     1621 taaagctcca aaatctttct tctatcttcc atgttaggca atccaacata caatcttcta
     1681 tcaaatctac ctgctcttaa aagtgcatca tctaaaacct ctatcttatt tgttgcagca
     1741 attacaatta caccactctc tccatcaaat ccatccattt gagttagaag ttcatttaaa
     1801 gtagattctc tctcatcatt agattttcca cttctcatct ttccaacagc atctatttca
     1861 tctataaata caattgatgg tgcatttatt tttgcacttg agaaaagttc tcgtactttt
     1921 tttgcaccca ttccaacata aatatgaaca aaactagctc cactttggta aaaaaatggc
     1981 acttgtgcct cacctgcaac tgctcttgca ataagagttt ttccaacacc t
//
LOCUS       DAOQAN010000201         2029 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_82423_length_2029_cov_3.637791, whole genome shotgun sequence.
ACCESSION   DAOQAN010000201 DAOQAN010000000
VERSION     DAOQAN010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82423_length_2029_cov_3.637791"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..171)
                     /locus_tag="PKO10_03925"
     CDS             complement(<1..171)
                     /locus_tag="PKO10_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014473124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNW66329.1"
                     /translation="MAVKITDICISCDACLDECPVGAIVDNDDNPTGEDVYYVYKDKC
                     VECVGHNDAPACA"
     gene            339..935
                     /locus_tag="PKO10_03930"
     CDS             339..935
                     /locus_tag="PKO10_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014473125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNW66330.1"
                     /translation="MLVTKKAPDFTATAVLADGSISEDFNLYKNIGKNGAVLFFYPLD
                     FTFVCPSEIIAFSNRIKEFEDRGIQVIGCSVDSQFSHFAWRETPVENGGIGRVKYPLV
                     ADITKQISKDYDVLFGDSVALRGSFLIDKDGTVRHAVINDLPLGRNIDEMIRMVDTML
                     FTNEHGEVCPAGWSKGDEGMKADKSGVAEYLAKHSSDL"
     gene            1094..>2029
                     /gene="rho"
                     /locus_tag="PKO10_03935"
     CDS             1094..>2029
                     /gene="rho"
                     /locus_tag="PKO10_03935"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HNW66331.1"
                     /translation="MEESKEDLKSKNSKKTRTHIPVDGYKIEQLRELPIETLIDIAND
                     LDVENPQELKRQDLMFMILASQIDAGGFILFTGILEIKDGGFGFLRAIDGNFSDTSND
                     SYVSATQIKKFALRTGDIVTGQVRPPNKDSEKYNALLKIEAINYLPVKDSKNRPLFDN
                     LTPLYSTTKFKFEYDSQKLTGRVLDLFAPMGKGQRSLIVAPPKTGKTELLKELAHAIS
                     KNHPEVTLMVLLIDERPEEVTDMQRSVKGEVYSSTFDLPAQNHVRVAEIVIEKAKRLV
                     EMKKDVVILLDSITRLARAYNTVTPSSGKVLSGGVD"
BASE COUNT          784 a          275 c          314 g          656 t
ORIGIN      
        1 tgcacatgct ggagcatcat tgtgaccaac acactctaca catttatctt tatatacata
       61 atatacatct tcacccgttg ggttatcatc attatcaact attgctccaa ctggacactc
      121 atctaaacat gcatcacagc taatacaaat atcagtaatc tttactgcca tatctattct
      181 ccataaaaaa ttttataaaa ctctatcaaa gtaaaaataa atatattatt aaagataaag
      241 attatctttt gaaaaatatt tttattttta agcttactct aaaaaaatag tgatataatt
      301 tcgacattaa attttaaaaa aataaaaaag gattaaaaat gttagtaaca aaaaaagctc
      361 cagattttac agctacagct gttttagcag atggttcaat ttcagaagat tttaacttat
      421 ataaaaatat cggaaaaaat ggtgctgtac tatttttcta cccattagac tttacttttg
      481 tttgtccatc agaaattatt gcgttttcaa atagaattaa agagtttgaa gatagaggaa
      541 ttcaagtaat tggttgttca gttgattctc aattctcaca ctttgcatgg agagaaactc
      601 cagttgaaaa tggtggaatt ggaagagtta aatatccatt agttgcagat attacaaaac
      661 aaatctcaaa agattatgat gttttatttg gtgattcagt tgcattaaga ggttctttct
      721 taattgataa agatggaaca gtaagacatg cagttattaa tgatttacca cttggaagaa
      781 acattgatga gatgattaga atggttgata ctatgctatt tacaaatgag catggtgaag
      841 tttgtccagc tggttggtca aaaggtgacg aaggaatgaa agctgataaa tctggtgttg
      901 ctgaatattt agctaaacac tcttctgatt tataattata aattcaaatt aaaaagagag
      961 taaagttttt actctctttt ttacttttct tgacaaattt ataaatatta actaaaatca
     1021 catttcactt tttttaaaag ctacaaaaac tacaaaaata catatattat gtaaattcaa
     1081 aggtaaaaaa ctcatggaag agtcaaaaga agatttaaaa tcaaaaaata gtaaaaaaac
     1141 aagaactcat ataccagtag atggatataa aatagagcag ctaagagaac ttccaattga
     1201 aactctaatc gacatagcta atgatttaga tgttgaaaac ccacaagaat taaaaagaca
     1261 agatttaatg tttatgatac ttgcttctca aatagacgca ggtggattta ttctttttac
     1321 tggtatttta gagataaaag atggtggatt tggtttttta agagcaattg atggaaactt
     1381 ttctgatact tcaaatgact cttatgtaag tgctactcaa attaaaaaat ttgcactaag
     1441 aactggagat attgtaacag gacaagtaag accaccaaac aaagatagtg aaaaatataa
     1501 tgcgctactt aaaattgaag caataaacta tcttcctgta aaagattcaa aaaatagacc
     1561 tctatttgat aacttaactc ctttatattc aactacaaaa tttaaatttg aatacgattc
     1621 acaaaaatta actggaagag ttttagatct ttttgctcct atgggtaaag gtcaaagaag
     1681 tttaattgta gcacctccaa aaactggtaa gacggaactt ttaaaagaat tagctcatgc
     1741 aataagtaaa aatcacccag aagttacatt aatggtttta ttaattgatg agagacctga
     1801 agaggtaaca gatatgcaaa gaagtgttaa aggtgaagtt tatagctcta cttttgactt
     1861 accagctcaa aaccatgtta gagttgctga aatagttatt gaaaaagcca aaagacttgt
     1921 agagatgaaa aaagatgttg taattttgct tgattctatt acaagattag caagagctta
     1981 taatactgta acaccatcat ctggaaaagt tctttcaggt ggagttgat
//
LOCUS       DAOQAN010000202         2009 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_83703_length_2009_cov_2.682190, whole genome shotgun sequence.
ACCESSION   DAOQAN010000202 DAOQAN010000000
VERSION     DAOQAN010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83703_length_2009_cov_2.682190"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1080
                     /locus_tag="PKO10_03940"
     CDS             <1..1080
                     /locus_tag="PKO10_03940"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HNW66332.1"
                     /translation="FTAADIWMSIERTQKFIEATNRGWEYALKNKEEIIDILIKKYGV
                     NKSKEALLYERDVVNQVMMRDFYPIGKVSPELTQRLVKQLSYSGMIEPNQKINHIFFE
                     NIVDKIPSDFSLTKSEKEYLNSKHSLKICIDPFWYPIEFMKDGKISGITSDLKRYFEE
                     KIEINIDVVPTKNWNESLDFIKDKKCDIISSISPSYDRMSYLNFTKPILTLPIVVTTQ
                     KDKPFLRDISLLKNEKIAILKGHFIAEYIKDYFPYLKIVEVASMNEGLYLVEQGEVYG
                     YIDNALVLSSTIQKEFSNSLKIGFRFDILDELSIGTRNDEPILNDIFSRLVDDLDETK
                     KQEFLNNWTIITEQVGWFSLKEIIF"
     gene            1131..1649
                     /locus_tag="PKO10_03945"
     CDS             1131..1649
                     /locus_tag="PKO10_03945"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNW66333.1"
                     /translation="MKILNKKLKELYLTDKLTGLYNRFKIDKELSSQKDNIDRNDSYS
                     CGLILIDIDYFKSINDTLGHLVGDCILKDISKLLKNNLRKTDIIGRWGGEEFLIILPF
                     TSKDIAKKVAENLRALIEENNFSYKMNRKITISIGVTEFSKSKSVEDTLLLVDNLLYK
                     AKENGRNRVEES"
     gene            complement(1646..>2009)
                     /locus_tag="PKO10_03950"
     CDS             complement(1646..>2009)
                     /locus_tag="PKO10_03950"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNW66334.1"
                     /translation="AEQNETSQKQQSSIQENTNNKKNKNLESEYLAKVKNKIEKNKVY
                     PKVAKRLNQTGKVIVSFDILKDGKVTNIKIINKSKFEKLDEASIELLTNIGFFEAIPN
                     ELNKTVWNIQIPINYQIN"
BASE COUNT          767 a          196 c          308 g          738 t
ORIGIN      
        1 ttcactgctg ccgatatttg gatgtcgatt gagagaactc aaaaatttat tgaagcaaca
       61 aatcgtggtt gggagtatgc tttaaaaaat aaagaagaga ttatagatat tttaataaaa
      121 aaatatggag ttaataagtc aaaagaagcg cttctttatg agagagatgt tgtaaatcaa
      181 gttatgatga gagattttta tcctattgga aaagtaagtc cagagctaac gcaaagattg
      241 gtaaaacagc taagttattc tggtatgata gaaccaaatc aaaaaataaa tcacatcttt
      301 tttgaaaata tagtagataa aattcctagt gatttttctt taactaaaag tgaaaaagag
      361 tatttaaata gtaaacatag tttaaagatt tgtattgatc cattttggta tccaatagag
      421 tttatgaaag atggaaaaat ttctggaata acatctgatt taaaaagata ttttgaggaa
      481 aaaattgaaa taaatataga tgtagtacct acaaaaaact ggaatgaatc tcttgatttt
      541 ataaaagata aaaagtgtga tattatatct tcaatttctc ctagctatga tagaatgagc
      601 tacttaaatt ttacaaaacc aatattaact cttcctattg tagttacaac acaaaaagac
      661 aaaccttttt taagagatat ttcacttctt aaaaatgaaa aaatagctat tttaaaagga
      721 cattttattg ctgaatatat taaagattat tttccatatt taaaaatagt tgaagttgca
      781 tcaatgaatg aagggcttta tttagtagaa caaggagaag tttatggata tattgataat
      841 gcgcttgttt tatcttcaac aatacaaaaa gagttttcaa atagtttaaa aattggcttt
      901 agatttgata ttttagatga actctcaata ggaacaagaa atgatgaacc tattttaaat
      961 gatatatttt caagacttgt agatgattta gatgaaacaa aaaaacaaga gtttttaaat
     1021 aactggacca ttattacgga acaagttggt tggtttagtt taaaagagat tatattttag
     1081 ttatatttac aactactatt tttggtggat tgatttttta tcaaagaaag ttgaaaatac
     1141 taaataaaaa gcttaaagaa ttgtatttaa cagataagtt aacaggactt tataatagat
     1201 ttaaaataga taaagagctc tcttcgcaaa aagataatat agatagaaat gatagttact
     1261 cttgtggttt aatacttatt gatattgatt attttaaaag tataaatgat actttagggc
     1321 atttagtagg agattgtatt ttaaaagata tttcaaagtt attaaaaaat aatctcagaa
     1381 aaacagatat tattggaaga tggggaggag aagagttttt gattattctt ccattcactt
     1441 caaaagatat tgcaaaaaaa gttgctgaaa atttaagagc tcttattgaa gagaataatt
     1501 tttcttataa gatgaataga aaaataacaa ttagcatagg agtaacagag ttttcaaaga
     1561 gtaaaagtgt agaagatact cttttactcg tagataatct actttataaa gctaaagaaa
     1621 atggaagaaa tagggtagaa gagtcttaat ttatttggta gtttatagga atttgaatat
     1681 tccaaactgt tttatttaat tcatttggaa tggcttcaaa aaatcctata tttgtaagaa
     1741 gttcaatgct tgcttcatcc aatttttcaa attttgattt attaataatt tttatatttg
     1801 taacttttcc atcttttaaa atatcaaaag atactatcac ttttccagtt tgatttaatc
     1861 tttttgcaac ttttggatag actttattct tctctatctt attttttact tttgctaaat
     1921 attcactctc tagattttta ttttttttgt tgtttgtatt ttcttgaata gaagattgtt
     1981 gtttttgtga agtttcattt tgttcagct
//
LOCUS       DAOQAN010000203         2007 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_83837_length_2007_cov_2.262295, whole genome shotgun sequence.
ACCESSION   DAOQAN010000203 DAOQAN010000000
VERSION     DAOQAN010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83837_length_2007_cov_2.262295"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..115)
                     /locus_tag="PKO10_03955"
     CDS             complement(<1..115)
                     /locus_tag="PKO10_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit N-terminal domain-containing protein"
                     /protein_id="HNW66335.1"
                     /translation="MSKDTNRRDFIGYSFAAVAAVGGAASLYGMKQVWDPLP"
     assembly_gap    382..391
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(392..1748)
                     /gene="thrC"
                     /locus_tag="PKO10_03960"
                     /pseudo
     CDS             complement(392..1748)
                     /gene="thrC"
                     /locus_tag="PKO10_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147945.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
     gene            complement(1761..>2007)
                     /locus_tag="PKO10_03965"
     CDS             complement(1761..>2007)
                     /locus_tag="PKO10_03965"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014475228.1"
                     /note="catalyzes the phosphorylation of
                     N-acetyl-L-glutamate to form N-acetyl-L-glutamate
                     5-phosphate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HNW66336.1"
                     /translation="TLDKATVEMFKKDGTIAGGMIPKVDSCIEAIHNGVNKAHIIDGR
                     VEHSILLELFTSDGIGTQFIRVDNPNNGIDIEKLLNS"
BASE COUNT          649 a          328 c          235 g          785 t
ORIGIN      
        1 taggaagtgg atcccatacc tgtttcatac catacaaaga tgcggctcct ccaactgcag
       61 caactgcagc aaatgagtag ccaataaaat ctcgtctatt tgtatcttta gacatatttg
      121 gcttcctttt tttaaaatta gaatcggctc tgattttaat ataaagatac ttaagaaaga
      181 ttgattttgg tcaattttat aaacgaaaga ttaatgaaat gtgtaaaaat agaatattat
      241 aaaaatagtt taatttgctt cttattagaa aatcttatga ttttctaata aattttatta
      301 tttcagattc aatattttct ttgtttatta ctaaagagtg atttatttta gcattaaaaa
      361 gatttttaat agattgaggt annnnnnnnn naactaatct cttctaaagc ctctttatca
      421 ctatactttt tagagtcttg ctttaaagca tttaaaacag ttggtgagaa ttttgtccac
      481 tctgctgttg agtagattac agtttttagt ggtttttctc ttaagtcatt gtaagctttt
      541 atgcaagttg cagtatgagg atccattaaa taaccactat ctaaaaactc ttttattgta
      601 gtgttaccaa atgagtcatt actaaaagta gcactaaaat atttttgtaa ctcttttgtc
      661 tcattttgag tcatttcaaa aatattattt ttatttaaat cttccattaa ctcttttgtt
      721 ctactacttc caaatagatc aaaaattact ctttctatgt ttgaagattt taaaatatcc
      781 atagcaggtg attttgtaag ttttagagct ttatctctga tatcataaac tcctgtgttt
      841 atccattgtg ttaaaatgtt attttcattt gaagccacta aaagtttctc tatatttaat
      901 cccatctttt gagcataata agctccaaga acgtttccaa aatttccact aggcactact
      961 aaatatatat tttcaccatt tttaatagca ttttgacgaa taagttctaa atatgaccag
     1021 aagtgataga ttatttgaaa aataattctt ccaaagttaa cactatttgc agcacttaat
     1081 ttaatatcat ctttttccaa ctctgcttta aaactacttg aagctaaaag agattttaga
     1141 gcattttgtg catcatcaaa gtttcctttt attcctaaaa cttttagatt ttctccatct
     1201 tcacaaacca tttgaagtct ttgaacatca gaagttcctc catctggata tagacaaaca
     1261 acttgaatat tttttttatt tctaaaagta tttaaagctg ctggtcctgt atctccagaa
     1321 gttgctgcta gtattaggta attttcacct cttttttgtg caatagagct aaaaatactt
     1381 ccaaaaggtt gaagagccat atctttaaaa gcacgtgtag gaccgtgata ctgctcattt
     1441 acatataaat catctttaac ttttacaaca ggacttggat ttaaaggatt atcaaaatta
     1501 tcatataaat ctaatgcttt ttgtatttca ctatcatcaa tatcaatctc aaaagctttt
     1561 aaaatatcaa aagctaactc tttataactt ttgtttatat ggttttctat aaagttatca
     1621 cctaaatttg gtaaattttt tggtacataa agtccaccaa aagagctact tggatttaaa
     1681 atagcttcac taaagcttac ttcttttgct cttttaccat catttcctct tgtttcaata
     1741 aatcccatac catcttcctt ttaactattt aataattttt ctatatcaat tccattattt
     1801 ggattatcaa ctcttataaa ttgagttcct attccatcac ttgtaaataa ctcaagtaat
     1861 attgagtgtt caactctacc atctataata tgagctttat ttacaccatt atgaatagcc
     1921 tcgatacaag aatcaacttt tggaatcatt ccaccagcaa ttgttccatc ttttttaaac
     1981 atttcaacag ttgctttatc taaagtt
//
LOCUS       DAOQAN010000204         1997 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_84429_length_1997_cov_2.682801, whole genome shotgun sequence.
ACCESSION   DAOQAN010000204 DAOQAN010000000
VERSION     DAOQAN010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84429_length_1997_cov_2.682801"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /locus_tag="PKO10_03970"
     CDS             <1..617
                     /locus_tag="PKO10_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014473343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNW66337.1"
                     /translation="NLPVTSHFLESKDEKDWLNKDEGSFLDFFKNFLNQEKATTKPLE
                     FLNLFKGVKNLSFTHCVEASNEDFSKIKELKATINHCVSSNRFLNNSRLNLESLGNIP
                     FSIGTDGLSSNNSLSMFDELRVALYTHYERNIIKFSKTLLNAATVNGAKALGVKKGSL
                     IKDFDADIIGFCLPDKIEEVEDIYMQIILHTKYVDKIVIGGEFV"
     gene            610..1494
                     /gene="sppA"
                     /locus_tag="PKO10_03975"
     CDS             610..1494
                     /gene="sppA"
                     /locus_tag="PKO10_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HNW66338.1"
                     /translation="MFDFLKKLFLPVIWFLDFITKYFKTIVFLTIVYFIFFSTAEDET
                     IAKYNANLQKIDLIGQIIDPSKVLEDIERASNDSNIKGVLFVIDSPGGAVAPSVEIAY
                     AIKELSMKKPVVAYASGTIASGSYYASIWADKIIANPGSIVGSIGVIMQGFEASKLLE
                     NIGISSQTIKAGKYKESGTFTRKWTNDEEQELQGVINSTYNMFISDVADARKLDIKKH
                     TSFADAKIFTAYQAKDVGLVDEVATLNFAKYSLIELSKVEKPIWKKEDKFEKFMDKLM
                     SQAISKVVMSFSSSLKAY"
     gene            complement(1530..>1997)
                     /locus_tag="PKO10_03980"
     CDS             complement(1530..>1997)
                     /locus_tag="PKO10_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013134564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HNW66339.1"
                     /translation="SYIHSGAMAVALGERINDDEKGLLLFRNEKRPLWIADLREVLDG
                     MVNGDDRCRLAIDMVAYKIKSYVGAYVAALNGIDALCFTGGIGENASLIREKVCAGLD
                     AMGLVIDPTKNNKRSSEARDISTNASPARIFVIPTQEEYVIANDTYNIVSKGR"
BASE COUNT          747 a          247 c          320 g          683 t
ORIGIN      
        1 aaaatcttcc agttacttct cattttttag aatcaaaaga tgaaaaagat tggttaaata
       61 aagatgaagg aagtttttta gattttttca aaaatttttt aaatcaagag aaagcaacaa
      121 caaaaccttt ggagttttta aaccttttta agggtgtaaa aaatctatct tttactcatt
      181 gtgtagaagc aagtaatgaa gattttagca agataaaaga gttaaaagca actataaatc
      241 attgtgttag ttcaaataga tttttaaata atagtagatt aaatttagag agtctaggaa
      301 atattccttt tagcatagga acagatggtt taagttcaaa taattcatta agtatgtttg
      361 atgagctaag agttgcactt tatactcact atgaaagaaa tataataaag ttttcaaaaa
      421 ctttgttaaa tgctgcaact gtaaatggag caaaagcttt aggagttaaa aaaggtagtt
      481 taataaaaga ttttgatgct gatattatag gtttttgttt accagacaag attgaagaag
      541 ttgaagatat ttatatgcag attattttgc atacaaaata tgtagataaa attgtgatag
      601 gaggagagtt tgtttgattt tttaaaaaaa ctatttttac cagttatttg gtttttagat
      661 tttattacga aatattttaa aacaatagtt tttttaacta ttgtatattt tatatttttt
      721 agtacagctg aagatgaaac aattgcaaaa tataatgcaa atctacaaaa gattgattta
      781 ataggacaga taattgatcc ttcaaaagta ctagaagata tagaaagagc ttcaaatgat
      841 tctaatataa aaggtgtttt atttgtaata gatagtccag gaggtgctgt tgcaccttct
      901 gttgagatag cttatgcaat aaaagagttg agtatgaaaa aaccagttgt tgcatatgca
      961 agtggaacaa ttgcaagcgg tagttactat gcttctattt gggccgataa aattattgca
     1021 aatcctggaa gtattgttgg ctctatcggt gttattatgc aaggttttga agctagtaaa
     1081 ttattagaaa atattggaat tagttctcaa actataaaag ctggaaaata taaagaatca
     1141 ggaacattta caagaaaatg gacaaatgat gaagaacaag agcttcaagg tgttataaat
     1201 agtacatata atatgtttat aagcgatgtt gcagacgcta gaaaattaga tattaaaaaa
     1261 catacaagtt ttgcggatgc aaaaatattt acagcatatc aagcaaaaga tgttgggctt
     1321 gtagatgaag ttgcaacact taattttgca aaatatagtt taatagagtt atcaaaagtt
     1381 gaaaaaccta tttggaaaaa agaggacaaa tttgaaaaat ttatggataa acttatgagt
     1441 caagctattt caaaagttgt tatgagtttt tctagcagtt taaaagcata ttaaaatttt
     1501 attaaaaggc gaaattttcg ccttttaaat tatcttcctt tagaaactat attgtaagta
     1561 tcatttgcaa ttacatactc ttcttgagtt ggaattacaa atattctagc aggtgatgca
     1621 tttgtagaga tatctcttgc ttcacttgat cttttgttat ttttagttgg atcaattaca
     1681 agccccattg catcaagacc tgcacaaact ttttctctaa ttaaagaggc attttcacca
     1741 attccacctg taaagcaaag tgcatcaata ccatttaatg ctgcaacata agctccaaca
     1801 taagatttga ttttatatgc aaccatatca atagcaagcc tacatctatc atcaccattt
     1861 accataccat ctaaaacttc tcttaaatca gcaatccata aaggtctttt ttcatttcta
     1921 aataaaagca aacctttttc atcatcgtta attctttcac ctaaagctac agccatggct
     1981 cctgaatgga tataaga
//
LOCUS       DAOQAN010000205         1995 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_84590_length_1995_cov_1.704124, whole genome shotgun sequence.
ACCESSION   DAOQAN010000205 DAOQAN010000000
VERSION     DAOQAN010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84590_length_1995_cov_1.704124"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1165
                     /locus_tag="PKO10_03985"
     CDS             <1..1165
                     /locus_tag="PKO10_03985"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNW66340.1"
                     /translation="LSKQANDKSFNQEEQTDEYFSKYFRPWKQSKLSYSEIEAKWGFS
                     YKNKKVYLENHNQATKEWFDKKIENANFENYNKDIKKAITLKNTNVRVLPTNSPMFYN
                     PSLPGEGFPFDYNQNSLLKINTPLIVSHFSKDRAWAFVESHFVGGWVEINNIAFVDDD
                     FIKDFTTNDYFIATKEKFAIYDPIFREYVKVGTIFPKKDNNFIVAKEDDNLNAKISYI
                     QIEEEFIEKMPLSYNHENRARILKEFINEPYGWAGLLNNRDCSSFTQDYFSVFGKYLH
                     RNSKAQTTNGKYFDISQLNLDEKKEFIRKNGVPFSTLVYLKGHIMLYIGLENNEPLVV
                     HNVWSVKLKDKEDKEFRYIIGKTAITTLEPAKEQEGFTQDSNILKKVLGITIL"
     gene            complement(1423..>1995)
                     /gene="rsmH"
                     /locus_tag="PKO10_03990"
     CDS             complement(1423..>1995)
                     /gene="rsmH"
                     /locus_tag="PKO10_03990"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014474091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HNW66341.1"
                     /translation="GFSFNSENLDMRMNQNQSLDASTVINSYSQVELENIFKEYGEIR
                     EYKKIASLIVQNRPFYSAKELAEFFYNKLPKGKIHPATLIFQAIRIEVNDELGVLDRL
                     FKSMEEAKLKDCIVAIISFHSLEDRVVKNYFKKWSENCICPKDAFRCECGKNHALGKI
                     ITKKPIIATNIEIKQNPRSRSAKLRVFRFV"
BASE COUNT          778 a          262 c          261 g          694 t
ORIGIN      
        1 actatcgaaa caagcaaatg ataaatcatt caatcaagaa gaacaaacag atgaatattt
       61 ctctaaatat tttagacctt ggaaacaatc aaaattatct tatagtgaaa ttgaggcaaa
      121 atggggattt tcatataaaa ataaaaaagt atatttagaa aatcataatc aagcaacaaa
      181 agagtggttt gataaaaaaa tagagaatgc aaattttgaa aactacaata aagatattaa
      241 aaaagcaatt acgctaaaaa atacaaatgt aagagtattg ccaacaaact caccaatgtt
      301 ttataatcct agtttaccag gagaaggttt tccatttgat tacaaccaaa attcactact
      361 aaaaataaat actcctttga tagtttctca cttctcaaaa gatagagctt gggcatttgt
      421 tgagtcacac tttgttggtg gttgggtaga gataaataac attgcatttg tagatgatga
      481 ttttataaaa gattttacta caaatgatta ttttatagca actaaagaaa aatttgctat
      541 ttatgaccct atttttagag agtatgtgaa agttggtaca atttttccta aaaaagataa
      601 taattttata gttgcaaaag aagatgataa tttaaatgca aaaatatcat atattcaaat
      661 agaagaagag ttcattgaaa aaatgccatt atcttataat cacgaaaacc gtgctagaat
      721 tttaaaggag tttataaatg aaccttatgg ttgggctggg cttttaaaca atagagattg
      781 ctctagtttt actcaagatt attttagtgt atttggaaaa tatcttcaca gaaactcaaa
      841 agcacaaaca acaaatggaa agtattttga tatttcacaa ctaaatcttg atgaaaaaaa
      901 agagtttata agaaaaaatg gtgtcccttt ttctacttta gtatatttaa aaggacacat
      961 aatgttgtat ataggtcttg aaaataatga acctttagtt gttcataatg tttggagtgt
     1021 taaattaaaa gacaaagagg acaaagagtt tagatatata atagggaaaa cggctattac
     1081 aactctagaa cctgcaaaag agcaggaagg ttttactcaa gatagcaata tactaaaaaa
     1141 agttttagga ataactattt tataaactat ttattttctt caagtctaat taaaggattg
     1201 aataaaagag aatctaaaac ttgattctta aacttcatat cctctaattg tttacttaaa
     1261 aatgtatttt cttaatttag agatatataa tgtgaataga gtctatttat atctttactt
     1321 gtataataaa tattattgct aacataaatt tttgggatat acataaaaag tgctaaaaat
     1381 agcataataa taacaaatct taatgaattt ttttcattta atttaaacaa atctaaatac
     1441 cctcaacttt gcacttctac ttcttggatt ttgttttatc tcaatattgg ttgcaattat
     1501 aggcttttta gtaataattt tacctaaagc atgatttttc ccacattcac atctaaaagc
     1561 atccttagga caaatacaat tttcgctcca ttttttgaaa tagtttttta caactctatc
     1621 ctctaaagag tgaaaagaga ttattgcaac tatacaatct tttagttttg cctcttccat
     1681 agatttaaat aatctatcca aaacacctaa ctcatcattt acttcaattc taattgcttg
     1741 aaaaatcaaa gtagctggat gaatttttcc ttttggaagt ttattataaa aaaactctgc
     1801 gagctctttt gcactataaa atggtctatt ttgaacaatc aaagatgcaa tttttttata
     1861 ttctctaatt tcaccatact ctttaaaaat attttcaagt tctacttgag agtatgaatt
     1921 tataacagtt gaagcatcta aactctgatt ttgattcatt ctcatatcta gattttctga
     1981 attaaaacta aaacc
//
LOCUS       DAOQAN010000206         1987 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_85091_length_1987_cov_2.627847, whole genome shotgun sequence.
ACCESSION   DAOQAN010000206 DAOQAN010000000
VERSION     DAOQAN010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85091_length_1987_cov_2.627847"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            84..317
                     /locus_tag="PKO10_03995"
     CDS             84..317
                     /locus_tag="PKO10_03995"
                     /inference="COORDINATES: protein motif:HMM:NF021253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HNW66342.1"
                     /translation="MLALNQKQLLDEVELLPIDIKTKIVDKILSSLNPTDKTITDLWI
                     KEAKERKNQIELNQTELINGDEVFRKIAKKFDK"
     gene            314..607
                     /locus_tag="PKO10_04000"
     CDS             314..607
                     /locus_tag="PKO10_04000"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNW66343.1"
                     /translation="MKYSFHFEAEIELNSYIDYYEEFKVGLGLEFANEVYKTIQRIIN
                     FPNAWQILTDDLRRSLTNRFPFGVIYYIKNDEIIILAVMHLRKKPDYFLKRKR"
     gene            755..1612
                     /gene="rhuM"
                     /locus_tag="PKO10_04005"
     CDS             755..1612
                     /gene="rhuM"
                     /locus_tag="PKO10_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012013190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
                     /protein_id="HNW66344.1"
                     /translation="MQDLSNLVVYNDGELELKVSVDSETIWLTQLQMSQLFDTSTDNV
                     GLHLKNIYLEKELDENSTTEFFSVVRKEGNRLVKRELKHYNLDVIICVGYRVSSLRAT
                     KFRQWATSVLKNYIQNGHAINNHKITEQRLFALENDMQFIKSKIKNNSLEFNQNIFFD
                     GQIYDSYSFVNDLIKLAKSEIVLIDNYIDDTVFTLFSKYPNINFTIYTSTISKQLKLD
                     FEKYSKQYKNISLKTFKSSHDRFLIIDKKEIYHLGASLKDLGKKWFAFSKMSLNSLNL
                     DDILHKLEV"
     gene            1683..>1987
                     /locus_tag="PKO10_04010"
     CDS             1683..>1987
                     /locus_tag="PKO10_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66345.1"
                     /translation="MVSYTQNELLSITDFTKQISKILTDVKERSLEKVGILKNNRLEA
                     VVISTQEYEKLKKLEKFLEYLEHKEIFEVVEQRKKEPKDSYISLEDMAEKFDINLDN"
BASE COUNT          846 a          213 c          288 g          640 t
ORIGIN      
        1 tagaaaatag ctatttgaaa aagcttaaaa tataaaaatc aaatatgata taatcagcaa
       61 cttaatttaa aaaaggattt aaaatgttgg cattaaatca aaaacaacta ttagatgaag
      121 tagaactttt accaatagat ataaaaacaa aaattgttga taaaatatta tctagcttaa
      181 atccaacaga taaaacaata actgatttat ggataaaaga ggcaaaagag cgaaaaaatc
      241 aaattgaatt aaatcaaaca gagcttataa atggagatga agtttttaga aaaattgcta
      301 aaaagtttga taaatgaaat actcttttca ttttgaagca gaaatagagt taaatagcta
      361 tatagattat tatgaagagt ttaaagtcgg cttaggttta gagtttgcaa atgaagttta
      421 taaaactata caaaggataa taaacttccc aaatgcttgg caaattttaa ctgatgattt
      481 aagaagaagc ttgacaaata gattcccatt tggtgtgatt tattatataa aaaatgatga
      541 aataattatc ttagctgtaa tgcatcttag aaaaaaacca gattatttct tgaaaagaaa
      601 gcgataaaaa attgttagta ttttttgtag taaaccttaa ctacaaaaat ctttaaaata
      661 taaaaatcaa atttaattac aaattgtaat attcctttct ctttttactc tcttttgcta
      721 agatttaaaa aatcttcaag aaagaggaaa caaaatgcaa gatttatcaa atttagtggt
      781 ttacaatgat ggtgaactag aactcaaagt ttcggttgat agtgaaacaa tttggctaac
      841 acaattacaa atgtctcagt tatttgatac aagtacagat aatgttggtt tgcacttaaa
      901 gaatatatat ttagaaaaag agctagatga gaattcaact accgagtttt tctcggtagt
      961 tcgaaaagaa ggaaatagat tagtaaaaag agaacttaag cattataatt tggatgtaat
     1021 tatttgtgtt ggttatagag taagctcttt aagagccact aaatttagac aatgggcaac
     1081 atctgtgtta aaaaactata ttcaaaatgg acatgcgata aataatcaca aaattacaga
     1141 acaaaggctt tttgcacttg aaaatgatat gcaatttata aaatcaaaaa taaaaaacaa
     1201 ctctttggaa tttaatcaaa acatcttttt tgatgggcaa atttacgatt cttacagttt
     1261 tgtaaatgat ttaataaaac ttgcaaagag tgaaatagtt ttgatagata actatattga
     1321 tgatacagtt tttactcttt tttctaaata tccaaatata aatttcacaa tctatacttc
     1381 aacaatttca aaacagctaa aactagattt tgaaaaatat tcaaaacaat acaaaaatat
     1441 ctctctaaaa acttttaaat ctagccacga tagatttttg attatcgata aaaaagagat
     1501 ttatcatttg ggagcaagtt taaaagattt gggtaaaaaa tggtttgcct tttcaaagat
     1561 gagtttgaac tctttaaatt tagatgatat tttacataag ctagaagtgt gaaaatcaaa
     1621 tatttatata ttttttatgg taaatttact ataatatttc atattaaatt taggagtaaa
     1681 ttatggtaag ttatacacaa aatgaacttt tatcaattac agatttcaca aaacaaataa
     1741 gcaaaatcct aacagatgta aaagagagaa gcttagaaaa agtcggaatt ttaaaaaaca
     1801 atcgacttga agcagtcgtt atctctacac aagagtatga aaaactaaaa aaacttgaaa
     1861 agtttttaga atatttggag cataaagaga tttttgaagt tgtggaacaa agaaaaaaag
     1921 agccaaaaga cagttatatc tctttggaag atatggcaga gaagtttgat ataaatttgg
     1981 ataactt
//
LOCUS       DAOQAN010000207         1986 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_85155_length_1986_cov_2.469705, whole genome shotgun sequence.
ACCESSION   DAOQAN010000207 DAOQAN010000000
VERSION     DAOQAN010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85155_length_1986_cov_2.469705"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="PKO10_04015"
     CDS             <1..370
                     /locus_tag="PKO10_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66346.1"
                     /translation="NYIKNVINAYDGDIEKMMDLLKELNLTTVNQLDAINYEDFQLLI
                     KQRGSFKVAFEDIMFSTKVAIEKKEDFYDFVTKLIDNGFSNMAYNYLEGFQDYFFQDK
                     EIVKLYKRLEEKQNETKHKQ"
     gene            348..1634
                     /locus_tag="PKO10_04020"
     CDS             348..1634
                     /locus_tag="PKO10_04020"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008339083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HNW66347.1"
                     /translation="MKLSINNKIFTDNTNELEKDNIFVVSKQNEKFKDIAINGGFELL
                     NSSELKNYLDMSSIKIIGVTGTNGKTTTASTIYKILLNLGYKVALQGTRGFFINEICV
                     EDYSLTTPVQLGNFINIQKAIQNSCQYFVMEVSSHAIEQNRIEGLSFALKIHTNITRD
                     HLDYHKTIEEYIRVKNLFLSDESKKLINIDDKVVKFNPTNAFTYSLENSSNFKVLKYE
                     LKDNMNVEFLYENKNYKFSTNMMGIFNIYNLIASIAAVHITTNEKLENICKVVENFNS
                     ISGRMEIVSTKPLVIVDFAHTPDGMDEVLKSFPNKEIICVFGAGGNRDALKRPLMGEV
                     ASKYSKHIVVTSDNPRFEEPKKIIEDILNGIDDKSNVIVIEDRKEALKKAISLANDKS
                     VVLILGKGDERAQIIGDKKFEFSDKDEVLKILEVRD"
     gene            1891..>1986
                     /locus_tag="PKO10_04025"
     CDS             1891..>1986
                     /locus_tag="PKO10_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014768649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HNW66348.1"
                     /translation="MVTMKDLLECGVHFGHQTRRWNPKMAPYIFAE"
BASE COUNT          829 a          200 c          323 g          634 t
ORIGIN      
        1 aaattatata aaaaatgtaa taaatgctta tgatggtgat attgaaaaga tgatggattt
       61 gttaaaagag ttaaatttga caacagtaaa tcagcttgat gctattaatt atgaagattt
      121 tcaactttta ataaaacaaa gaggctcttt taaagtagct tttgaagata ttatgttttc
      181 aacaaaagta gctattgaaa aaaaagaaga tttttatgat tttgtaacaa aacttataga
      241 taatggtttt agtaatatgg cttataacta tcttgaagga tttcaagact acttttttca
      301 agataaagag atagtaaaac tttacaaaag attggaagag aaacaaaatg aaactaagca
      361 taaacaataa aatatttaca gacaatacaa atgagttaga aaaagataat atttttgtag
      421 tatcaaagca aaatgaaaag tttaaagata tagcaataaa tggtggtttt gaactattaa
      481 attctagtga gctaaaaaac tatttagata tgagttctat aaaaattatt ggagttacag
      541 gtacaaatgg taaaacaaca acagcttcaa ctatatataa gattctttta aatttgggat
      601 ataaagtagc tttgcaaggt acaagaggat tttttataaa tgagatttgt gtagaagatt
      661 attcactaac aactccagtt caacttggaa attttataaa tattcaaaaa gctatacaaa
      721 actcatgtca atattttgta atggaagtaa gttctcatgc aattgagcaa aatagaattg
      781 agggattaag ctttgcttta aagattcata caaatattac aagagatcat cttgattatc
      841 ataaaactat cgaggagtat ataagagtaa aaaatctatt tttgagtgat gaaagcaaaa
      901 aattaataaa tatagatgat aaagttgtta aatttaatcc aacaaatgcg tttacttatt
      961 cattagaaaa tagttcaaac tttaaagttc taaaatatga gttaaaagat aacatgaacg
     1021 tagagttttt atatgaaaat aaaaactata aattctctac aaatatgatg gggattttta
     1081 atatttataa tctaatagct tcaatagcag cagttcatat tactacaaat gaaaaactag
     1141 aaaatatttg taaagttgta gagaatttta attcaataag tggaagaatg gagattgttt
     1201 caacaaaacc tcttgtaata gtagattttg cacatactcc agatggtatg gatgaagttt
     1261 taaaaagttt cccaaataaa gagataattt gtgtttttgg agcaggtgga aatagagatg
     1321 ctttaaaacg accacttatg ggagaagttg caagtaaata ttcaaagcat atagttgtaa
     1381 ctagtgataa tccaaggttt gaagagccaa aaaaaataat tgaagatatt ttaaatggaa
     1441 tagatgataa atcaaatgtt atagtaattg aagacagaaa agaggcttta aaaaaagcta
     1501 taagtttagc aaatgataaa agtgttgttc taattcttgg caagggtgat gaacgagcac
     1561 aaataattgg agataaaaag tttgaattta gtgataaaga tgaggtcttg aagattttag
     1621 aagttagaga ttagttctag cttatatcaa acttttttaa tattttttag ataaaagttt
     1681 ttataatcat aaattgacta ttttaaaaat taaacaaatc ttaataaatt ttagctataa
     1741 tcgcagttct atttttcaaa ttttgaaact tagtacctca catgtatcaa tccttgctcg
     1801 ggttgtgtta gattaaaaac tagcattaag ggatacagag gctaaaccct agaaaaaaat
     1861 aaaaacaaac ataattcaag gagaattaac atggttacaa tgaaagacct attagagtgc
     1921 ggtgtacact tcggacacca aacaagaaga tggaatccaa aaatggcacc gtatatattc
     1981 gccgaa
//
LOCUS       DAOQAN010000208         1981 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_85468_length_1981_cov_3.218069, whole genome shotgun sequence.
ACCESSION   DAOQAN010000208 DAOQAN010000000
VERSION     DAOQAN010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85468_length_1981_cov_3.218069"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="PKO10_04030"
     CDS             complement(<1..446)
                     /locus_tag="PKO10_04030"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HNW66349.1"
                     /translation="MARILLNKDNLFYNLEAISQKATSKEKVAVVLKDNAYGHGIVEI
                     AKLSCEFGIKKAVVRSVEDAQKIKDYFSYILILAPNVFHNYSHTFHIALNTLENIEKI
                     PENSNVHIKIDTGMHRNGISIDDIEVAILGLLKQKANITGVFTHHKG"
     gene            complement(439..>1981)
                     /gene="uvrC"
                     /locus_tag="PKO10_04035"
     CDS             complement(439..>1981)
                     /gene="uvrC"
                     /locus_tag="PKO10_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HNW66350.1"
                     /translation="VLLRDDKTYPYIYVDLNEDIPRLEITSNIEKGKNIKYFGPYSSG
                     AKDMLDSIYEIVPLVQKKSCIKGKSACLFYQIGKCKAPCEQKITKEEYKKLLDEALSY
                     IYNKSKLKEKMKIYSDDFRFEDALNLRDRVKTIEKSEIKTGIDLATNEDLDIFAIKSA
                     SKKAVIVRMFIRDGKLASSNYDFIKINDDLEFDLNEAYKRAIVNYYSNELPIIPKEII
                     VADEILDLDDIEEFLYKRFNKKIKVINPKIDKKASIVKIALNNCDELLRIEASKNQTT
                     IYEELKELFCLQTTPSLIESFDNSHLMGQATVGAMIVWNENLGSFDKKAFRHYNLESK
                     DEYSQMREMLIRRVESFSKNPAPDLWIIDGGETLLKLAFDIVQSIGVNLDIIAISKQK
                     VDAKAYRAKGNAKDIVHFRKNGEYKSLNLLTSDKRLQFIQRQRDEAHRFAISFHKKQK
                     RKEDKEISFLQINGIGEAKIKKLLLYFGEFEKIKNASLDELKAVLNEVDSKNIFDYFR
                     KEENG"
BASE COUNT          590 a          327 c          207 g          857 t
ORIGIN      
        1 cctttatggt gtgtgaaaac ccctgtaata ttggcttttt gtttcaaaag ccctaaaata
       61 gccacttcta tatcatctat tgatattcca ttacggtgca ttcccgtatc tattttgata
      121 tgaacattac tattttctgg tattttttct atgttttcaa gagtgtttaa tgctatgtga
      181 aaagtatgtg aatagttgtg aaaaacatta ggtgctagga ttaaaatata agaaaaataa
      241 tcctttattt tttgcgcatc ttcaacactt cttacaactg cttttttaat accaaactca
      301 catgatagtt ttgcaatttc aactattcca tgaccataag cattatcttt taaaactaca
      361 gcaactttct ctttacttgt tgctttttgg cttattgctt ctagattata aaaaaggtta
      421 tccttgttta ataatattct agccattctc ttcctttcta aaataatcaa atatattttt
      481 tgaatcaact tcatttaaaa cagcttttag ctcatctaaa cttgcatttt ttatcttttc
      541 aaactctcca aaatatagaa gaagtttttt gattttcgct tcacctattc catttatttg
      601 taaaaaggat atctctttat cctcttttct tttttgtttt ttatgaaaac ttatagcaaa
      661 tctatgtgct tcatctcttt gtctttgaat aaactgtaat cttttatcac ttgtaagaag
      721 atttaagctt ttgtattcac cattttttct aaaatggact atatctttcg catttccttt
      781 tgctctatat gctttggcat ctactttttg ttttgaaatt gcaataatat ctaaatttac
      841 cccaatactt tgtacaatat caaatgctag ttttagcaaa gtttctccac catcaataat
      901 ccataaatca ggagcaggat tttttgaaaa gctttcaact cttcttatta gcatctctct
      961 catttgtgaa tattcatctt ttgattcaag gttatagtgt ctaaaagctt ttttatcaaa
     1021 agagcctaaa ttttcattcc aaacaatcat agctccaact gttgcttgtc ccatcaaatg
     1081 cgaattgtca aaactttcta ttaaactagg agttgtttgt aggcaaaata gttcttttaa
     1141 ctcttcatat atagttgttt gattttttga agcttcaatt cttagaagtt catcacagtt
     1201 gtttaatgca atctttacaa tactagcttt tttatctatt tttggattta ttacttttat
     1261 ttttttatta aatcttttat ataaaaactc ttcaatatca tctaaatcca aaatttcatc
     1321 agctacaata atctcttttg gaataattgg aagttcattt gagtagtagt ttacaattgc
     1381 tcttttgtat gcttcattta aatcaaactc taaatcatca tttattttta taaaatcata
     1441 atttgaagat gcaagttttc catctcttat aaacattctt acaatcacag cttttttact
     1501 agcagatttt atagcaaata tatctaaatc ttcatttgtt gctaaatcta ttcctgtttt
     1561 aatttcagat ttttcaatag tttttactct atctcttaaa tttaaagcat cttcaaaacg
     1621 aaaatcatct gaatatattt tcatcttctc ttttagtttt gatttattgt atatatagct
     1681 taaagcttca tctaaaagtt ttttatactc ttcttttgtt attttttgtt cacaaggagc
     1741 tttacatttt cctatttggt aaaatagaca agcactcttt ccttttatac aagatttttt
     1801 ttgcaccaat ggaactattt catatatgct atctaacata tcttttgctc cacttgagta
     1861 tggaccaaaa tattttatat ttttaccttt ttctatattg cttgttattt caagtcttgg
     1921 aatatcttca tttaaatcaa cataaatata tggatatgtt ttatcatctc ttaaaagtac
     1981 a
//
LOCUS       DAOQAN010000209         1981 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_85480_length_1981_cov_2.763240, whole genome shotgun sequence.
ACCESSION   DAOQAN010000209 DAOQAN010000000
VERSION     DAOQAN010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85480_length_1981_cov_2.763240"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1518)
                     /locus_tag="PKO10_04040"
     CDS             complement(<1..1518)
                     /locus_tag="PKO10_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008341139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(958..960),aa:Sec)
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNW66351.1"
                     /translation="MSANTYEALNAKVGRRSFLKMAAVSTAFGVTSSFASTVATRAAT
                     EEEIKNPFPGSKIVKTICTACSVGCGVIAEVQNGVWVRQEVAQDHPVSLGGHCCKGAD
                     MIDMVKSEVRLKYPMVKKNGQWQRISWDEALNNIASKLEDLRQKDGPDSTMFLGSAKF
                     NNEQAYYYRKFAAMFGTNNIDHQARIUHSSTVAGVANTWGYGAMTNSLGDIQNSKAII
                     IFGANPAVNHPVGFQHFLKAKERNNAKIIVVDPRFTKTAAKADLYAQIRPGTDIPFMY
                     GMLNIIFENGWHDKNFINDRVYGMEDIMAEAKNWPIERVADVTGVKADLIVQITKLYA
                     ASKPGTLVWAMGLTQHTIGTSNTRMAPILQLALGNMGVAGGGTNILRGHDNVQGATDF
                     GCLSDSLPGYYGLAEGSWKYFASSWGVDFEWLKGRFKDPSWMGKNGFTLARWWAGVLA
                     GNEGEDAIHNAGTNLKALFVMGNGITSTAQTKKVKEALDKLELAVFCDQFVNEGAI"
     gene            complement(1537..1749)
                     /locus_tag="PKO10_04045"
     CDS             complement(1537..1749)
                     /locus_tag="PKO10_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008338606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat pathway signal protein"
                     /protein_id="HNW66352.1"
                     /translation="MQESRRAFAKKAAIITAAAVATTGTVVLAGNGETSVQDDSNNGV
                     VVGKSNKKEILYKKTMAWEEFYKNAK"
BASE COUNT          587 a          427 c          250 g          717 t
ORIGIN      
        1 aattgcaccc tcatttacaa attggtcaca aaatactgct aattctaact tatctaaagc
       61 ctcttttact ttttttgttt gagcagttga agtaattcca ttacccatta caaaaagtgc
      121 ttttaggtta gttccagcat tatgaatagc atcttcacct tcatttccag ctaaaacacc
      181 agcccaccaa cgtgcaagtg taaatccatt tttacccatc caagaaggat ctttaaatct
      241 tcctttaagc cactcaaaat caactcccca agaagaagca aaatatttcc atgaaccttc
      301 agctaaacca taatatcctg gaagtgaatc tgataaacaa ccaaagtctg ttgcaccttg
      361 aacattatca tgacctctta aaatattagt tccaccacca gcaactccca tatttccaag
      421 agccaattgt aaaattggtg ccattcttgt atttgaagtt cctattgtat gttgagttaa
      481 tcccatagcc cataccaaag ttcctggttt tgatgctgca taaagttttg taatttgaac
      541 aattaaatca gctttaactc ctgttacatc agcaactctt tcaattggcc aatttttagc
      601 ctctgccata atatcttcca taccataaac tctatcatta ataaagtttt tgtcatgcca
      661 accattttca aaaatgatat ttaacattcc atacataaat ggaatatcag tacctggtct
      721 aatttgtgca tataaatctg cttttgcagc agttttagta aatcttggat ctactacaat
      781 aatctttgca ttgtttctct ctttagcttt taaaaagtgt tggaaaccaa ctgggtgatt
      841 aaccgctgga tttgctccaa aaattataat tgctttagag ttttgaatat ctcccaaaga
      901 atttgtcata gcaccataac cccatgtatt tgccacaccg gcaactgttg agctatgtca
      961 aattctagct tgatgatcta tattgtttgt tccaaacata gcagcaaatt ttctatagta
     1021 atatgcttgc tcgttattaa attttgctga tcctaaaaac attgttgaat caggtccatc
     1081 tttttgtctt aaatcttcaa gttttgatgc aatattattt aaagcttcat cccatgaaat
     1141 tctttgccat tgaccatttt tcttaaccat tgggtatttt aatctaacct cagatttaac
     1201 catatcaatc atatcggcac ctttacaaca atgccctcca agtgatactg gatgatcttg
     1261 tgctacctct tgtcttaccc acacaccatt ttgaacttct gcaataacac cacaaccaac
     1321 agaacatgct gtacaaattg tttttacaat ttttgaacca ggaaaaggat ttttaatctc
     1381 ttcttctgta gctgctctag ttgctactgt acttgcaaaa gatgatgtaa ctccaaatgc
     1441 tgtagaaact gctgccattt ttaaaaacga ccgtcttcct acttttgcat ttaaagcttc
     1501 atatgtattt gctgacataa gcttctccta ctaaatttat tttgcgtttt tataaaactc
     1561 ttcccaagcc attgtttttt tgtatagaat ctctttttta ttagattttc caacaacaac
     1621 accattgttt gaatcatctt gaacagatgt ttcaccattt ccagccaata caacagttcc
     1681 agttgtagca actgcagcag ctgtgataat tgctgctttt tttgcaaaag ctcttctact
     1741 ttcttgcacg actgacctcc ttatagaaat taagcccctc taaacttaag agactttata
     1801 aaaaaagtta aacttccttt ataaaatccc taaaaaggaa taccttttta gtttttagga
     1861 cctaaagctt ttaatgctct atttctctct cttctttctc tttcctctgg agtaatatct
     1921 ccccagttat cagttacttg ttttttagct ttctcttttt ttagtggttt tgtaacctct
     1981 a
//
LOCUS       DAOQAN010000210         1979 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_85567_length_1979_cov_9.537422, whole genome shotgun sequence.
ACCESSION   DAOQAN010000210 DAOQAN010000000
VERSION     DAOQAN010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85567_length_1979_cov_9.537422"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            69..1580
                     /locus_tag="PKO10_04050"
     CDS             69..1580
                     /locus_tag="PKO10_04050"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HNW66353.1"
                     /translation="MFIRKRKNPSGSISIQIIDKSNGKYKVVETIACVKDKKELEFYL
                     AKAESRLKQLNPNLFDAVEFNEKKHRFIGIKNKEISVVGPDIIFGYIMEYIGCDKVLK
                     KLKEKELFKQLVLSRIIDPGSKLNLIDYLYIYKHQNLDKNEIYRFLDTLSLNLKDKIE
                     QCIFEYTSMVTGGIRVSFYDVTTLYFEASSEDDLRKVGFSKDGKFTKPQIILGLLTSL
                     EGYPLGFEIHEGNCYEGKTFIPMLEKFQKKFNIEKPIVVADSGLLSKANILELEKLNY
                     RYILGARIKSSSSEIKNKIVSLNLNDEKNIETIYFSKNQKMIVSYSQQRAKKDRYTRT
                     KNYEKLKIKIKSGNLTKAHLNNKGYNKYLVLSDSQTTISIDEDKYNYDSSFDGLKGFV
                     TNDFSLNPTQIIEHYTNLWHIERAFRISKTDLKIRPIYHRLEHRIYSHILISFVAYTV
                     YKEFDRKLKINNISIKRKNAIGFIKSMYGIVHDNEISLLELSPEQEQIYKLFY"
     gene            complement(1569..>1979)
                     /locus_tag="PKO10_04055"
     CDS             complement(1569..>1979)
                     /locus_tag="PKO10_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66354.1"
                     /translation="TPLETIIKKCQEELGVNISNWIELDSEISSSGIEIVYFLTTLNE
                     GEIKKLQSQTDERAELFSINNLPTNILQDLKIQIERQFFKPKNKMNRKTKLLIYILIP
                     IFIILLSLMIVGKIKTGSFFLTFSNRHPILGLIK"
BASE COUNT          837 a          195 c          268 g          679 t
ORIGIN      
        1 ttaagggatt ttaattttaa atttgggtgt ctagcctatt gattatctct tattagctac
       61 aattttatat gtttattcgt aaaagaaaaa atccaagtgg cagtattagt attcaaatta
      121 ttgataagag taatggtaaa tataaagttg ttgaaacaat agcatgtgta aaagataaaa
      181 aagagttaga gttttatctt gcaaaagcag aatcaagatt aaaacaactt aatccaaatt
      241 tatttgatgc agttgaattt aatgagaaaa aacatagatt tattggtata aaaaataaag
      301 agatttcagt tgtaggacca gatattatat ttggatatat aatggaatat ataggctgtg
      361 ataaagtact taaaaaatta aaagaaaaag agttgtttaa acagctagtt ttatcaagaa
      421 taattgatcc aggtagtaaa ttaaatctta tagattattt gtatatttat aaacatcaaa
      481 atcttgataa aaatgagata tatagatttt tagatacatt aagtttaaat ttaaaagata
      541 aaatagaaca atgtatattt gaatatacat caatggtaac tggtggaatt agagtaagtt
      601 tttatgatgt aacaacattg tactttgaag catcaagtga agatgattta agaaaagtag
      661 gatttagtaa agatggaaaa ttcacaaaac cacaaataat tttaggactc ttaacttcat
      721 tagaaggtta tcctttagga tttgaaatac atgagggtaa ttgctatgaa ggtaaaactt
      781 ttattccaat gcttgaaaag tttcaaaaga aatttaatat agaaaaacca atagttgtag
      841 ctgatagtgg attactaagt aaagcaaata tattagaact agaaaaattg aattatagat
      901 atattttagg tgctagaata aaatcaagct caagtgaaat aaaaaataaa atcgtatcac
      961 taaatttaaa tgatgagaaa aatattgaga caatatattt ttcaaaaaat caaaagatga
     1021 tagtttcata ttcacaacaa agagcaaaaa aagatagata tacaagaaca aaaaattatg
     1081 agaaattaaa aataaaaata aaatcaggta atcttacaaa agcacattta aataataagg
     1141 gttataataa atatttagta ctttcagatt ctcaaacaac aatatcaata gatgaagata
     1201 aatataatta tgattcaagc tttgacggat taaaagggtt tgttacaaat gatttttcat
     1261 taaatccaac acaaataatt gaacattaca caaatctttg gcatatagaa agagctttca
     1321 gaatttctaa aacagattta aaaattagac caatatatca tagattagaa catagaatat
     1381 attcacatat tttaatttca ttcgttgcat atacagtata taaagagttt gatagaaaat
     1441 taaaaataaa taatatttca attaaaagaa aaaatgcaat aggatttata aaatctatgt
     1501 acggaatagt tcatgataat gaaatttcat tattagaatt atctccagaa caagaacaaa
     1561 tatataaact attttattaa acctaatatt gggtgtctat ttgagaaagt caagaagaaa
     1621 ctacctgttt taatttttcc aacaatcatt aaagagagta aaataataaa tataggaatc
     1681 aaaatataaa taagcaattt tgtttttcta ttcatcttat tttttggttt aaaaaattgt
     1741 ctctctattt gaatttttaa atcttgaagt atatttgtag gaagattatt tatagaaaat
     1801 agttctgctc tttcatctgt ttggctttgt agctttttta tttcaccttc atttaaagta
     1861 gtcaaaaaat atacaatctc tataccactt gatgatattt cactatctaa ctctatccaa
     1921 tttgaaatat ttacacctaa ctcctcttga cattttttta ttattgtttc aagtggagt
//
LOCUS       DAOQAN010000211         1978 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_85683_length_1978_cov_2.806032, whole genome shotgun sequence.
ACCESSION   DAOQAN010000211 DAOQAN010000000
VERSION     DAOQAN010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85683_length_1978_cov_2.806032"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="PKO10_04060"
     CDS             <1..528
                     /locus_tag="PKO10_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HNW66355.1"
                     /translation="IISTYYIIAAAEASANLARFDGVRFGARKGDGNLKDMYVDTKTQ
                     GFGYEVKKRIMLGSFVLSSGYYDAYYIKAQKVRSIIKAEFDKIFESSDLILAPVAPTT
                     APKFGSYSNALEMYLADIYTIGVNLAGLPAISLPVAKDSEGLPIGLQLIANSYEEQTL
                     FDGALSMEKAVKYTK"
     gene            552..>1978
                     /gene="guaB"
                     /locus_tag="PKO10_04065"
     CDS             552..>1978
                     /gene="guaB"
                     /locus_tag="PKO10_04065"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013135234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HNW66356.1"
                     /translation="MRIRKRALTFEDVLLVPAKSAVLPKEVCTKTKLTKNIELNVPFV
                     SAAMDTVTEYQAAIAMARLGGIGIIHKNMDIEAQVLQCQKVKKSESGMIIDPITIKPE
                     QTLQDAEDIMATYKISGVPVVDENGILVGILTNRDMRFTKDYRFKVYEKMTKMPLVTA
                     VEGTTLEQAAEIMHQNKIEKLPIVNKDNKLIGLITIKDINKKIEYPNACKDEFGRLRV
                     GAAIGVNQLDRARALVAVGVDVLVLDSAHGHSKGILDTVKAIKAELKVELIAGNVATA
                     EATRDLIAAGADGVKVGIGPGSICTTRIVAGVGVPQMSAIDECAIEAKKTETPIIADG
                     GIKYSGDVAKALAVGASCVMMGSALAGTDECPGEVILYQGRKFKSYRGMGSIGAMTKG
                     STDRYFQEGTAADKLVPEGIEGRVAYRGTIADIIHQMVGGLRSSMGYLGSKDIETFQD
                     TAEFVEITSAGLRESHVHDVTITNEA"
BASE COUNT          741 a          227 c          413 g          597 t
ORIGIN      
        1 ataatttcaa catattatat tattgcagca gcagaagcta gtgcaaactt ggctagattt
       61 gacggtgtta gatttggagc aagaaaaggc gatggaaact taaaagatat gtatgtagat
      121 acaaagacac aaggttttgg atatgaagtt aaaaaaagaa ttatgctagg ttcttttgtg
      181 ttaagttcag gatattatga tgcttattat ataaaagctc aaaaagttag aagtattata
      241 aaagctgagt ttgataaaat ttttgaaagt tctgatttaa ttttagctcc agttgctcca
      301 actactgctc caaaatttgg atcatattca aatgctttgg aaatgtattt agcagatatt
      361 tatactattg gagttaattt agcaggtctt ccagctatta gcctaccagt tgcaaaagat
      421 agcgaaggac ttccaatagg acttcaatta attgcaaata gttatgaaga acaaacttta
      481 tttgatggtg ctttatctat ggagaaagct gtaaaatata caaaataata aaatataatt
      541 aggatataaa gatgagaatt agaaaaagag ctttaacttt tgaagatgtg ttacttgttc
      601 ctgcaaaatc agcagtttta ccaaaagagg tttgtacaaa aacaaaatta actaaaaata
      661 ttgaattaaa tgttcctttt gtaagtgctg caatggatac agttacagag tatcaagctg
      721 cgattgcaat ggcaagactt ggtggaattg gaataattca taaaaatatg gatattgaag
      781 cacaagtttt acagtgtcaa aaagtgaaaa aatctgagtc aggaatgata attgacccaa
      841 ttacaataaa accagagcaa actttacaag atgctgaaga tattatggct acatataaaa
      901 tatctggagt tcctgttgtt gatgaaaatg gtattttagt tggtatttta acaaacagag
      961 atatgagatt tacaaaagat tatagattta aagtttatga aaaaatgaca aaaatgccac
     1021 tagttacagc agtagaaggt acaacactag agcaagctgc tgaaattatg catcaaaata
     1081 aaatagaaaa actaccaatt gtaaataaag ataacaaatt aattggtctt attacaataa
     1141 aagatataaa taaaaaaata gagtatccaa atgcatgtaa agatgagttt ggaagactta
     1201 gagttggagc tgctattggt gttaatcaac tagatcgtgc tagagcttta gttgcagttg
     1261 gagttgatgt tttagtttta gactctgcac atggtcattc aaaaggtatt ttagatactg
     1321 taaaagctat aaaagctgag ttaaaagttg agttaatagc tggaaatgtt gcaactgctg
     1381 aagcaacaag ggatttaatt gcagctggag ctgatggtgt taaagttgga attggacctg
     1441 gaagtatttg tacaacaaga attgttgcag gtgttggtgt tcctcaaatg agtgctattg
     1501 atgagtgtgc tattgaagct aaaaaaactg aaacaccaat aattgcagat ggtggaatta
     1561 aatattcagg tgatgttgca aaagcattag cagtaggtgc tagttgtgtt atgatgggaa
     1621 gtgcattagc aggaacagat gagtgtccag gtgaagtaat tctttatcaa ggaagaaaat
     1681 tcaaatctta cagaggaatg ggaagtattg gtgctatgac aaaaggaagt actgatagat
     1741 atttccaaga aggaactgct gctgataaac tagtacctga aggaatagaa ggaagagttg
     1801 cttatagagg aactattgct gatattattc atcaaatggt tggtggttta agaagttcta
     1861 tggggtattt aggaagcaaa gatatcgaaa ctttccaaga tacagcagaa tttgttgaaa
     1921 ttacaagtgc aggacttaga gaatctcatg ttcatgatgt aacaattaca aatgaagc
//
LOCUS       DAOQAN010000212         1974 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_85938_length_1974_cov_2.835852, whole genome shotgun sequence.
ACCESSION   DAOQAN010000212 DAOQAN010000000
VERSION     DAOQAN010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85938_length_1974_cov_2.835852"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="PKO10_04070"
     CDS             complement(<1..248)
                     /locus_tag="PKO10_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HNW66357.1"
                     /translation="MIKKVLFVTLIIILIFMISIEKTKQQDEILIASSMPQSSGLKAW
                     GDAVFTATNSYFNYANDNNLIKDKKIVLKVLDDKYEPD"
     gene            394..>801
                     /locus_tag="PKO10_04075"
     CDS             394..>801
                     /locus_tag="PKO10_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012013146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HNW66358.1"
                     /translation="MQEFIYYNKNGLDFPLSEKIQVTTSLDDIKEKSFLISNSKDVNS
                     EFIASEIDFYIKNSQDNLSKKIQNVLKLYEINATRFDFAQDISHSVDVSKEVLLITKT
                     QEEYDLFTSQLSKDDFELFRIDETILKTISGEIG"
     assembly_gap    802..811
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <812..>1974
                     /locus_tag="PKO10_04080"
     CDS             <812..>1974
                     /locus_tag="PKO10_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012013146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNW66359.1"
                     /translation="VVVKDSHKDITLRVSQIVWFDAKPSPKERIGVFDPNLSSINEVV
                     KILKDNINSFSYRKFTTYDKTICQYDGRREVICSKCEEVCPTVAITKDDTTKTLTFSQ
                     VDCHGCGGCISVCPSGALDYTPTNKESLYEISKFYKDTHPLIIPSKMSLDNLNIELKE
                     NILPFAIDGEKFLHEGTLLTLLQISGSQLIFYSDFLSKGTKDAIRILNDIYQKRYQKD
                     AILVAMNEDELILALKEVSFVENSYFNFNQDGFKKREVFSHRLQKIVGNDDLGVVETG
                     EHIHYAKVEVNQDTCTLCLVCVGACNVGALVADASDNTLRINPSICTACGYCEISCPE
                     TNCLTIKQDIIEYGFKYPERILGKITNQKNIYTLSISNYDKLKTSLIKKYIKE"
BASE COUNT          759 a          220 c          294 g          691 t
ORIGIN      
        1 aaatcaggtt catatttatc atcaagaact tttaaaacaa tttttttatc ttttatcaaa
       61 ttattatcat ttgcatagtt aaaataacta tttgtagcag taaaaacagc atctccccaa
      121 gctttcaaac cagaactttg tggcatagat gaagctatta atatctcatc ttgttgcttg
      181 gttttttcaa tagaaatcat aaatattaaa ataataataa gtgtaacaaa taaaactttt
      241 ttaatcattc ataagtttac attatttgtg tttaaaactc tcagtttaat tgtaataata
      301 tattctttaa tatatagaaa ttcaggttta tttaacttct ttaaatataa aatatggtaa
      361 aatatttttt actaaaaatt aagggcatag gttatgcaag agtttatata ctacaataaa
      421 aacgggttgg atttcccttt aagtgaaaag atccaagtaa ctactagttt agatgatatt
      481 aaagagaaaa gttttttaat atcgaactca aaagatgtga actctgaatt tattgcttct
      541 gagatagact tttatattaa aaattcacaa gataacttat caaaaaagat tcaaaatgtt
      601 ttaaaacttt atgagataaa tgctacaaga tttgactttg ctcaggatat ttcacatagt
      661 gtagatgttt caaaagaggt tttgttaatt acaaaaacac aagaagagta tgatttattc
      721 acatctcaat tatcaaaaga tgactttgaa ctttttagaa tagatgaaac tattttaaaa
      781 actattagcg gagaaattgg annnnnnnnn naagtagttg taaaagatag tcataaagat
      841 ataactttaa gagtttctca aattgtttgg tttgatgcaa aaccatctcc taaagagaga
      901 attggagttt ttgacccgaa cttaagtagc ataaatgaag tagttaaaat attaaaagat
      961 aatataaact ctttttcata tagaaaattt acaacttatg ataaaactat ttgtcaatat
     1021 gatggaagaa gagaggtaat ttgtagtaag tgtgaagagg tttgcccaac tgttgctatt
     1081 acaaaagatg atacaacaaa aactctaact ttctctcaag tagattgtca tggatgtggt
     1141 ggatgtatta gtgtttgccc aagtggagca ttagattata ctcctacaaa taaagagtca
     1201 ctttatgaaa tatcaaagtt ttataaagat acacaccctt taattattcc ttcaaaaatg
     1261 tctttggata atttaaatat agagcttaaa gagaatattt taccatttgc aattgatggt
     1321 gaaaagtttt tacatgaagg aactttgctt actttgcttc aaatatcagg ttcgcaactt
     1381 attttttata gtgatttttt gtcaaaaggt acaaaagatg ctattagaat attaaatgat
     1441 atttatcaaa aaagatacca aaaagatgca atattagttg ctatgaacga agatgaatta
     1501 attttggctt taaaagaagt atcttttgtg gaaaactctt attttaactt taatcaagat
     1561 ggcttcaaaa aaagagaagt tttttctcat agacttcaaa agattgttgg aaatgatgat
     1621 ttgggagttg ttgaaacagg tgaacatatt cattatgcaa aagttgaagt aaatcaagat
     1681 acatgtactt tatgtctagt ttgtgtaggt gcttgtaatg ttggagcttt agttgctgat
     1741 gctagtgata ataccctaag aataaatcca agtatttgta cagcatgtgg atattgtgaa
     1801 atttcttgcc ctgaaacaaa ttgtttaaca ataaaacaag atataattga atatggtttt
     1861 aagtatcctg aaagaatact tggtaaaata actaatcaga aaaatatata tactctaagt
     1921 atatctaatt atgataaact taaaactagt ttaataaaaa aatatataaa agaa
//
LOCUS       DAOQAN010000213         1969 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_86265_length_1969_cov_2.242947, whole genome shotgun sequence.
ACCESSION   DAOQAN010000213 DAOQAN010000000
VERSION     DAOQAN010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86265_length_1969_cov_2.242947"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1481
                     /locus_tag="PKO10_04085"
     CDS             <1..1481
                     /locus_tag="PKO10_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HNW66360.1"
                     /translation="YLRPGKVGEASVIGFILLILAIHYGSIIAADPYWKQVFTFDATT
                     LAFIMMAYGFIAAVLPVWFLLAPRDYLSTFLKIGVIVLMAIAIVIVAPDIQMPKMNTQ
                     YFDGSGPVFAGAIFPFLFITIACGAISGFHALISSGTSPKMLENETHALPVGYGSMLM
                     ESAVAIMALICATILHPGLYFAINSPAVFIGTDVVQVAQTISTWGFSVTPEEIFTLTK
                     NIGEETILSRTGGAPTFAIGVALVLHEIFGGVDMMGFWYHFAILFEALFILTAVDAGT
                     RACRFMVQDMLGNVYKPLGNTNNYAAGILATFLSVAGWGYFLYQGTIDPRGGIYSLWP
                     LFGVSNQMLAGMALMLATAILYKMGKAKYTWVTVLPATFVLVATMYGGIQKVLPFKEG
                     DRVHNAVSHVATAQIQIKKIADLEAKIPTLTVEADINKAKADIAIANKLKNSNIVNAV
                     LAVFFMFATTLVIIATLGICFGRIKIPLKETPYVRLDSLQKA"
     gene            1484..1681
                     /gene="kcuS"
                     /locus_tag="PKO10_04090"
     CDS             1484..1681
                     /gene="kcuS"
                     /locus_tag="PKO10_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_148936506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KCU-star family selenoprotein"
                     /protein_id="HNW66361.1"
                     /translation="MFGKIKEFYEKSEKFFHPLVGLSSYEKYLEHMKRVHPDKKVLTR
                     GEFFNESLDRKYNTGGFKKCC"
     gene            complement(1784..1960)
                     /locus_tag="PKO10_04095"
     CDS             complement(1784..1960)
                     /locus_tag="PKO10_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNW66362.1"
                     /translation="MFQREYPGLEQKISQEELSYRSNLHRTYIGAVERGEKNISLINM
                     NKIAKALKEKQVIL"
BASE COUNT          616 a          268 c          351 g          734 t
ORIGIN      
        1 gatatttaag acctggaaaa gttggagaag cttcagttat aggttttatt cttctaattt
       61 tagctattca ttatggaagt atcattgcag ctgaccctta ttggaaacaa gtatttacat
      121 ttgatgctac aactttagca tttataatga tggcatatgg atttatagct gctgttttac
      181 cagtttggtt cttattagct cctagagatt atttatcaac tttcttaaaa attggtgtta
      241 ttgtacttat ggctattgca attgttattg ttgctccaga tattcaaatg ccaaaaatga
      301 atacacaata ttttgatgga agtggtcctg tttttgcagg agcgatattc ccatttttat
      361 ttattactat tgcttgtggt gctattagtg gtttccatgc tctaatttca agtggaacaa
      421 gtcctaaaat gttagaaaat gagactcatg cacttcctgt tggatatggt tctatgttaa
      481 tggagagtgc agttgctatc atggctttaa tttgtgcgac aattttacat ccaggacttt
      541 attttgctat taactctcct gctgtattta taggaacaga tgttgtacaa gttgctcaaa
      601 caatctctac ttggggattt agtgtaactc ctgaagaaat tttcacactt acaaaaaata
      661 ttggtgaaga gacaatttta tcaagaactg gaggagctcc tacttttgct attggggttg
      721 cacttgtact tcatgaaatt tttggtggag ttgatatgat gggattctgg tatcactttg
      781 ctattctttt tgaagctcta tttattttaa ctgctgttga tgcaggaaca agagcttgta
      841 gatttatggt tcaagatatg ctaggaaatg tttataaacc tttaggaaat acaaataact
      901 atgcagctgg tattttagct acatttttaa gtgttgctgg ttggggatat tttctgtatc
      961 aaggaactat tgatccaaga ggtggaattt attcattatg gccactattt ggtgtaagta
     1021 accaaatgct agctggtatg gctttaatgc ttgcaactgc aatactatac aaaatgggta
     1081 aagcaaaata tacttgggtt acagtattac ctgcaacatt tgtattagtt gcaactatgt
     1141 atggtggaat tcaaaaagtg ttacctttca aagagggtga tagagttcat aatgctgtaa
     1201 gtcacgttgc tactgcacaa attcaaatca aaaaaatcgc tgatttagaa gcaaaaattc
     1261 ctactttaac agtagaagct gatataaata aagcaaaagc tgatatagct attgcaaata
     1321 aactaaaaaa ttcaaatata gttaatgctg ttttagcagt gttctttatg tttgcaacta
     1381 ctttagtaat tattgcaact ttaggaattt gttttggaag aataaaaata cctttaaaag
     1441 agactcctta tgtaagactt gattctcttc aaaaggctta aatgtgtttg gaaaaatcaa
     1501 agaattctat gaaaaatctg aaaagttttt tcaccctctt gtggggcttt ctagctatga
     1561 gaaatatctt gagcatatga aaagagttca tccagataaa aaagttttaa ctagaggcga
     1621 gttttttaat gagtctttag atagaaaata caatactggt ggttttaaaa agtgttgtta
     1681 gacttttaag agtagagttt ctactaaaca taaaaaggct tagaattttt ttctaagcct
     1741 ttttttatat ctttaaaata gtggaataat ttgttctaat aatctataaa ataacttgtt
     1801 tttcttttaa agcttttgca attttattca tatttattaa tgaaatgttt ttttcacctc
     1861 tttcaactgc accaatgtaa gttctgtgta aattagatct ataagacaat tcttcttgag
     1921 aaattttttg ttctaatccg ggatattctc tttgaaaaat ctcatatcg
//
LOCUS       DAOQAN010000214         1963 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_86656_length_1963_cov_2.751048, whole genome shotgun sequence.
ACCESSION   DAOQAN010000214 DAOQAN010000000
VERSION     DAOQAN010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86656_length_1963_cov_2.751048"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="PKO10_04100"
     CDS             <1..441
                     /locus_tag="PKO10_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66363.1"
                     /translation="GNNYTKKDDFFKKFPLYLIVLNHDSLAVFSELHKKNINENIVLV
                     ANISNTPWLIKQIAVNGELEKMFKNSKINLINDSSGSFSNSLDINNNSQNGYFVYKVF
                     EDGKITKIFQNSVKKGALQNGITKEEIETELENFIKEFNKYNIN"
     gene            complement(453..536)
                     /locus_tag="PKO10_04105"
     tRNA            complement(453..536)
                     /locus_tag="PKO10_04105"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(500..502),aa:Leu,seq:caa)
     gene            complement(645..920)
                     /locus_tag="PKO10_04110"
     CDS             complement(645..920)
                     /locus_tag="PKO10_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNW66364.1"
                     /translation="MNKAEFIDAVAAKAGLSKKDAKGAVDAVLDTITEALVKKDSVSF
                     IGFGTFSTAARAAREARVPGTDKTVKVAATTVAKFKVGKALKEAVAK"
     gene            complement(1019..1597)
                     /locus_tag="PKO10_04115"
     CDS             complement(1019..1597)
                     /locus_tag="PKO10_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase and adducin N-terminal
                     domain-containing protein"
                     /protein_id="HNW66365.1"
                     /translation="MLNQDTVKLLSELSLSMFTKNFFGIFQGAISAKLDHEHFMINSK
                     DAVFDNLDDKSFCTLNMNKPDYRWNIASLDAYTHSAIYTNIHEAKYIAFGMPIYTTAY
                     TLGHDNIIFEDYFGKILFGEVPIYNPGDLSTWKERSALEITKSIKNTDHNLLVIKGIG
                     TYIYDRDIHELVKKIAILENSCRLLSIKSTFR"
     gene            1731..>1963
                     /gene="mraW"
                     /locus_tag="PKO10_04120"
     CDS             1731..>1963
                     /gene="mraW"
                     /locus_tag="PKO10_04120"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:NF013919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytosine(1402)-N(4))-methyltransferase"
                     /protein_id="HNW66366.1"
                     /translation="MDCTTGYAGHSSGLLNQNSSVKLICNDQDDEALNFSKNRLKDFE
                     NRVEFNKGNFENIIKKFENYPIRGVLADIGVSS"
BASE COUNT          753 a          248 c          264 g          698 t
ORIGIN      
        1 ggaaataact atacaaaaaa agatgatttt ttcaagaaat ttccattata tttgatagtt
       61 ttaaatcatg attctttggc agtttttagt gaattgcata agaaaaatat aaatgaaaat
      121 atagttttag tagcaaatat atcaaataca ccttggctta ttaaacaaat agcagtaaat
      181 ggtgaacttg aaaagatgtt taaaaattca aaaattaatt tgataaatga ttcaagtggt
      241 agtttttcta atagtttaga tataaataat aactcacaaa atggatattt tgtatataaa
      301 gtttttgagg atggaaaaat aactaagatt tttcaaaata gtgtaaaaaa gggtgcatta
      361 caaaatggaa taactaagga agagatagaa actgaattag aaaattttat aaaagagttt
      421 aataaatata atataaattg aattaaaaca agtggtagcc ctagccagag tcgaactggc
      481 actccaatgg aacacgattt tgaatcgtac gcgtctacca attccgccat agagccacat
      541 attttaaaat aagtcttaat attaaataaa gtaaattata aatataaaaa aagaaaggag
      601 agtttatttt aaataaaaca aactctctta ataagtttta aaacttactt agcaacagcc
      661 tctttaagag cttttccaac tttaaattta gcaactgttg tagcagcaac ttttacagtt
      721 ttatcagttc ctggaactct agcttctcta gctgctctag ctgctgttga gaatgtacca
      781 aacccaataa agcttacaga atctttttta actaaagctt cagtaatagt atctaatact
      841 gcatcaacag caccttttgc atctttttta gataaaccag cttttgcagc aaccgcatca
      901 ataaattccg ctttgttcat aataactcct cgtataaaat aaattgcgaa aactttatta
      961 tagttaaact ttaagtttcc taaaattttt acaatttttt attaaatttt ataaaaaatc
     1021 atctaaaagt gctttttata cttaaaagtc tgcaagaatt ttccaaaatt gctatttttt
     1081 ttacaagttc atgaatatct ctatcgtaaa tataagtacc gataccttta ataacaagta
     1141 agttatggtc tgtatttttt atagattttg taatctctaa agcacttctc tctttccaag
     1201 ttgataaatc acctggatta tatattggaa cttctccaaa aagtattttt ccaaaatagt
     1261 cttcaaaaat tatattatca tgccctaaag tataagctgt agtataaatt ggcataccaa
     1321 atgctatata ttttgcttcg tggatatttg tataaatagc tgaatgagta tatgcatcta
     1381 aactagcaat attccatcta tagtctggtt tattcatatt taaagtacaa aaagatttat
     1441 catctaaatt atcaaaaaca gcatcttttg aatttatcat aaagtgttcg tggtctagtt
     1501 tggctgagat tgcaccttga aaaattccaa aaaagttttt tgtaaacatc gatagagata
     1561 attctgataa taattttact gtatcttgat tcaacatttt aagccttaaa tatattttcg
     1621 gtattatata ccaattttaa taaaaggtta aatttgcaaa atataccaca tattcctgtt
     1681 ttatataatg aagttttgat tgttttaaag atataaacaa aggttatatt attgattgta
     1741 ctacaggata tgcaggacat agtagtggat tattaaatca aaatagtagt gtaaaattaa
     1801 tctgcaatga tcaagatgat gaagcattga attttagtaa aaatagatta aaagattttg
     1861 aaaatagagt cgaatttaac aaaggaaatt ttgaaaatat tattaaaaaa tttgaaaatt
     1921 atccaataag aggtgttttg gctgatattg gagtatcttc ttt
//
LOCUS       DAOQAN010000215         1959 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_86958_length_1959_cov_2.549370, whole genome shotgun sequence.
ACCESSION   DAOQAN010000215 DAOQAN010000000
VERSION     DAOQAN010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86958_length_1959_cov_2.549370"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1180
                     /gene="purH"
                     /locus_tag="PKO10_04125"
     CDS             <1..1180
                     /gene="purH"
                     /locus_tag="PKO10_04125"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HNW66367.1"
                     /translation="NIDIGGPAMVRSAAKNFDSVIIVTDVADYDLVLNNLKNNTNTVE
                     FRRDLMIKAYEHTASYDSMIANYMNKRFNNGFGAKQFIVGSKVFDTRYGENPHQKGAL
                     YEFESQFSNKFKVIKGEPSFNNMGDISGAARIAASFGTDNAVCIVKHGNPCGFAIKDT
                     LLNSYVEALKCDPVSAFGGVVAVNGVVDKELAEKMNEIFLEVVFAADFTADAVEVFET
                     KKRIKLFSQGTKYLELANDSFDFKRVDGGFVYQEADKVADDEVKNSKLMSSRAASEQE
                     VKDMEIAYKIASLTKSNCVVYVKNSAMVAVGMGMTSRVDASKAALRKASDMGLDVNGA
                     VLASEAFFPFRDSIDEAQKAGVKCVIEPGGSIRDDEIIEAANEYGMALYFSGIRHFLH
                     "
     gene            1240..>1959
                     /locus_tag="PKO10_04130"
     CDS             1240..>1959
                     /locus_tag="PKO10_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNW66368.1"
                     /translation="MRLFLNLKLLFLVIFLNTNLYSKDLTKVTIQLSWFDQFQFAGYY
                     MAKEQGFYKDAGLDVHILPFSLGMNIPKMVNDGDVDFAIGRENLILEKAKYPKIIALA
                     AIFQATPLVLLTTKDSGIDTFKKFENKKLMRTKDDGSEVSLKAMLSASKIDLKSITQV
                     EHSHNIYDLIEKRVDIISAYTSKAPYILQKEQIKYNIFYPKDYGFDMYSDFLITNIDK
                     YNNDYNVVEKFKKASLKGWEYA"
BASE COUNT          741 a          219 c          354 g          645 t
ORIGIN      
        1 aaatattgat attggtggac cagcaatggt aagaagtgct gctaaaaact ttgatagtgt
       61 aattattgta acagatgttg ctgattatga tttagtacta aataatctta aaaacaatac
      121 aaacacagta gagtttagaa gagatttaat gattaaagct tatgaacaca cagcttctta
      181 tgactctatg attgcaaact atatgaataa aagatttaac aatggttttg gtgcaaaaca
      241 gtttattgtt ggatctaaag tatttgatac aagatatggc gagaatcctc atcaaaaagg
      301 tgctttatat gagtttgaat ctcaattttc aaataaattc aaagtaatca aaggagaacc
      361 tagttttaat aatatgggtg atattagtgg agctgctaga attgctgcat cttttggaac
      421 agataatgct gtttgtatag ttaaacatgg aaatccatgt ggatttgcta taaaagatac
      481 actattaaac tcttatgttg aagctttaaa atgtgaccca gttagtgcat ttggtggagt
      541 tgttgctgta aatggagtag ttgataaaga gttagcagaa aaaatgaatg aaattttcct
      601 tgaagttgtt tttgcagcag atttcactgc agatgctgtt gaagtttttg aaactaaaaa
      661 aagaatcaaa ctattctcac aaggaacaaa atatttagaa ctagcaaatg atagttttga
      721 tttcaaaaga gttgatggtg gttttgtata tcaagaggct gataaagttg cagatgatga
      781 ggttaaaaac tctaaactta tgtcttcaag agcagcaagt gaacaagaag taaaagatat
      841 ggaaattgct tacaaaatag caagtttgac aaaatcaaac tgtgttgtgt atgttaaaaa
      901 ctctgcaatg gtagctgttg gaatgggaat gacatctaga gttgatgctt ctaaggcagc
      961 acttagaaaa gctagtgata tgggattaga tgtaaatgga gcagtactag catctgaagc
     1021 atttttccca tttagagata gcattgatga ggcacaaaaa gctggtgtta aatgtgtgat
     1081 tgaaccaggt ggaagtatta gagatgatga aattatagaa gctgcaaatg agtatggtat
     1141 ggctttatat ttctcaggaa ttagacactt tttacactaa ctatttatat taaaattatc
     1201 tataccaaaa tattttggta tagattttaa aggcaaaaat tgagactttt tttaaatctt
     1261 aaacttcttt ttttagtaat ctttttaaat acaaacttat attcaaaaga tttaacaaaa
     1321 gttacaattc aactatcttg gtttgatcag tttcaatttg ctggttatta tatggcaaaa
     1381 gagcaaggtt tttacaaaga tgctggactt gatgttcata ttttgccatt ttctttaggt
     1441 atgaatattc caaaaatggt aaatgatgga gatgtagatt ttgcaatagg aagagaaaat
     1501 ctaattcttg aaaaagcaaa atatccaaaa attatcgctc ttgcagcaat ttttcaagca
     1561 acacctttag ttcttttaac aacaaaagat tcaggaatag atacatttaa aaagtttgaa
     1621 aataaaaaac ttatgagaac aaaagatgat ggaagtgaag tttcattaaa agctatgttg
     1681 tcagctagta aaatagattt aaaaagtata actcaagtag aacattcaca caatatttat
     1741 gacttgatag aaaaaagagt tgatattatc tctgcatata catcaaaagc accatatata
     1801 ttacaaaaag agcaaataaa atataatatt ttttatccaa aagattatgg ctttgatatg
     1861 tatagtgatt ttttgattac aaatattgat aaatacaaca atgattataa tgttgttgaa
     1921 aaatttaaaa aagcatcttt aaaaggttgg gaatatgct
//
LOCUS       DAOQAN010000216         1945 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_87951_length_1945_cov_2.427513, whole genome shotgun sequence.
ACCESSION   DAOQAN010000216 DAOQAN010000000
VERSION     DAOQAN010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87951_length_1945_cov_2.427513"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="PKO10_04135"
     CDS             <1..540
                     /locus_tag="PKO10_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNW66369.1"
                     /translation="GCYGDGGALFTNDEKLADKIKSLRLHGQSIRYHHQYIGMGGRLD
                     TIQAAVLNVKLKYYEKDLKLRQDVAEKYTKALNEKNIETPFVKSDRTSAWAQYSIRVQ
                     NRTELQTKLQNLGIPTAVHYPMPLHVQECFKYLNLKEGDFPISEKVSKEIMSLPMNPY
                     VSDEEIEFIVENLAKELRC"
     gene            534..1523
                     /locus_tag="PKO10_04140"
     CDS             534..1523
                     /locus_tag="PKO10_04140"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNW66370.1"
                     /translation="MLNVALVGFGYWGPNIARNINNNPNLNLHTICDMIDENLEKAHK
                     IYASSTNYEKDFNKVLEDKTIDIVAIATQTSSHYYLIKQALLAFKNVYVEKPFTATLQ
                     EAQELEELAKKQNKIIHIDHIMIFHPAIKKIKEIISSGEIGEVLFINSTRKSLGQFRK
                     DVSSMWDLAVHDLSIIDYLMDGKNPTSIKASGDKFYNPKESITFVNLKYENFSVHLES
                     NWLSPIKERNITIVGSKKMLVYEDLKLENKLTVYDKNVEVVSGENITDENYLVKTNEF
                     GTYSPYIKPEDALYNSIEHFRTSIINQTQSLSNPSQAIRVHKILEIADNNMNM"
     gene            1715..>1945
                     /locus_tag="PKO10_04145"
     CDS             1715..>1945
                     /locus_tag="PKO10_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014473583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNW66371.1"
                     /translation="MRIKMALSVASILVTQNLLLANETTKLDDVQVVTSASGYEQKIT
                     DAPASISVISQEDLKKKKYSNLAEAIEDVEGVD"
BASE COUNT          829 a          243 c          292 g          581 t
ORIGIN      
        1 ggatgctatg gtgatggtgg agcactcttt actaatgatg aaaaacttgc agataaaata
       61 aaatccctta gacttcatgg acaatcaatt cgttatcatc atcaatatat tggtatgggt
      121 ggaagacttg atactattca agcagcagtt cttaacgtaa aactaaaata ctatgaaaaa
      181 gatttaaaac taagacaaga tgtagcagaa aaatatacaa aagcactaaa tgaaaaaaat
      241 atagaaacac ctttcgtaaa aagtgataga acatcagctt gggcacaata ctcaataaga
      301 gtacaaaata gaactgaact acaaacaaaa cttcaaaacc taggaattcc aacagcagta
      361 cactacccaa tgccactaca tgtacaagaa tgctttaaat acctaaacct aaaagaaggt
      421 gactttccaa tctcagaaaa agtatcaaaa gagataatga gcttaccaat gaatccatat
      481 gtaagtgatg aagagattga gtttattgta gaaaatttgg caaaagagtt aagatgttaa
      541 atgtagcact tgtaggattt ggttattggg gaccaaatat tgcaagaaat ataaataata
      601 atccaaattt aaatcttcat acaatttgcg atatgataga tgaaaatcta gaaaaagcac
      661 acaaaatata tgcatcttca acaaactatg aaaaagattt taataaagtt ttagaagata
      721 aaactataga tatagttgct attgctacac aaacaagtag ccactattat ttaataaaac
      781 aagcactatt ggcttttaaa aatgtatatg ttgaaaaacc ttttactgct actttacaag
      841 aggcacaaga gcttgaagag ttagcaaaaa aacagaataa aattatacat atagatcata
      901 ttatgatttt tcatccagca ataaaaaaaa taaaagagat aatttctagt ggtgaaatag
      961 gagaagtttt atttataaat tcaactagaa agagtttagg acaatttaga aaagatgtaa
     1021 gctctatgtg ggatttagct gttcatgatt tatctataat tgattatcta atggatggta
     1081 aaaatccaac aagtataaaa gcaagcggtg ataaatttta taatccaaaa gagagtatta
     1141 catttgtaaa tctaaaatat gaaaactttt ctgtgcatct tgaatcaaac tggttaagtc
     1201 caataaaaga gagaaatatt actattgttg gctctaaaaa aatgcttgtt tatgaggatt
     1261 taaaattaga aaacaaactt acagtttatg ataaaaatgt agaagttgta agtggagaaa
     1321 atattacaga tgaaaactat ttggtaaaaa caaatgagtt tggaacttat tctccatata
     1381 taaaaccaga agatgcttta tataatagta tcgaacattt tagaacatca attataaatc
     1441 aaacacaatc tttatcaaat ccatctcaag caataagagt tcataaaatt ctagaaattg
     1501 cagataataa tatgaatatg taagatttaa tcttgcatat ttaaaaaaca ttttaataat
     1561 cactatcaat tttatcttaa tttaagaaaa aaccattaat attctatttc ctttttgtta
     1621 attataataa taattatcaa aaataaaata ggtttctcct aaaatctatt ttactaaatt
     1681 aaagtcgaaa aagaaaatat ggaaaaggaa tgaaatgaga ataaaaatgg cactaagtgt
     1741 agcttctatt ttagttacac aaaatttact tttggcaaat gagactacaa aacttgatga
     1801 tgtgcaagtt gtaacttctg cttcaggata tgagcaaaaa attacagatg caccagcatc
     1861 aatttcagtt ataagtcagg aagatttaaa aaagaaaaag tattctaatt tggctgaagc
     1921 tattgaagat gttgaaggtg ttgac
//
LOCUS       DAOQAN010000217         1921 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_89684_length_1921_cov_2.995713, whole genome shotgun sequence.
ACCESSION   DAOQAN010000217 DAOQAN010000000
VERSION     DAOQAN010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89684_length_1921_cov_2.995713"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..750
                     /locus_tag="PKO10_04150"
     CDS             <1..750
                     /locus_tag="PKO10_04150"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNW66372.1"
                     /translation="EIIDCDEISIGVFEGISRGLKPLLEFQDFAIFDKPTHLMVHPIS
                     KNTPYSLLDEIRYHFGENANLIHRIDAETSGLVIVGKNKKSEIELKDMFQEKKYHKSY
                     LAIVCGKIDKELKIDKNIDKEGLLIGVKMKTCQKEEGGKESITIIKPIKYNKEKDLTL
                     IEAIPLTGRQHQIRVHLHSIGHTILGDPIYGVDDVNAENYLNKTLSKEDRFKVTGSSR
                     LWLHANYLEFTYKNITYKIYSKNQDLKNEFF"
     gene            complement(766..>1921)
                     /locus_tag="PKO10_04155"
     CDS             complement(766..>1921)
                     /locus_tag="PKO10_04155"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HNW66373.1"
                     /translation="PTIGVVSNLNKKRDVQIELTDDVSKVLEDDSIDIVVELMGGIEK
                     PYEIVKRALENGKSVVTANKALLAYHRYELQELAGDSPFEFEAAVAGGIPIINALRDG
                     LSANNIKSIQGIMNGTCNYMLTKMINEGIAYDIILKEAQELGYAEADPTFDVGGFDTA
                     HKLLILGSIAYGIDVKPEDILIEGIQNISKTDIEFAKEFEYNIKLLGIAKKVDNQIEL
                     RVHPVLIPQDKMIAKVDGVMNGISVVGDKVGETMFYGAGAGGDATASAVIANIIDIAR
                     KGKGSPMLGFEKQVGVKPTLMPKDEIKTKYYLRLEVADKTGTLAKVAKVFGDNSISIE
                     NMMQKAKKDEKANLLLTTHTCVEKDILKAIYELEHSQVVLKKPAMIRIED"
BASE COUNT          678 a          309 c          261 g          673 t
ORIGIN      
        1 gaaatcattg attgtgatga gatttctatt ggagtttttg aaggaattag tagaggatta
       61 aaacctttat tagaatttca agattttgca atttttgata aaccaacaca tcttatggtt
      121 catcctatat ctaaaaacac tccttactct ttacttgatg aaataagata tcactttgga
      181 gaaaatgcaa atttaattca tagaattgat gctgaaacaa gtggtttagt aatagttgga
      241 aaaaataaaa agagtgaaat agagctaaaa gatatgtttc aagagaaaaa ataccataaa
      301 tcatatcttg caattgtttg tggaaaaata gataaagagt taaaaataga taaaaatatt
      361 gataaagagg gacttttaat tggtgttaaa atgaaaactt gccaaaaaga agagggtggt
      421 aaagagtcaa ttacaattat aaaaccaata aaatataaca aagaaaaaga tttgacactt
      481 attgaagcaa ttccactaac aggaaggcaa catcaaataa gagttcatct tcactcaatt
      541 ggacatacaa ttttaggaga ccctatttat ggtgttgatg atgtaaatgc agaaaattat
      601 ctaaataaaa ctctaagcaa agaggataga tttaaggtta caggctcaag taggctttgg
      661 cttcatgcaa actatttgga gtttacatat aaaaatataa cttataaaat atactcaaaa
      721 aatcaagatt tgaaaaatga gtttttttaa aaagagtaga agaatttaat cttctattct
      781 tatcatagca ggttttttaa gaactacttg agagtgttca agttcatata tagcttttaa
      841 aatatctttt tcaacacaag tgtgagttgt taaaagtagg tttgcttttt catctttttt
      901 tgctttttgc atcatatttt ctattgagat agaattatct ccaaaaactt ttgcaacctt
      961 tgctaaagta cctgttttat ctgcaacttc aagtcttaaa taatattttg ttttaatctc
     1021 atcttttggc attaaagttg gttttactcc tacttgtttt tcaaaaccaa gcataggtga
     1081 gcctttacct tttcttgcaa tatcaataat atttgcaata actgcgctag ctgttgcatc
     1141 tccacctgca cctgctccat aaaacatagt ttcaccaact ttatctccaa ctacagagat
     1201 tccattcata acaccatcaa cttttgctat catcttgtct tgaggaatta aaacagggtg
     1261 aactctaagc tctatttgat tatctacttt ttttgcaatt cctaggagtt ttatattata
     1321 ttcaaactct tttgcaaact ctatatctgt tttagagata ttttggatac cttcaattaa
     1381 aatatcttct ggttttacat ctattccata tgcaattgaa cccaaaatta aaagtttatg
     1441 agcagtatca aatccaccaa catcaaaagt tggatcagct tctgcataac ctaactcttg
     1501 agcttctttt aagataatat catacgcaat accttcatta atcattttag taagcatata
     1561 gttacaagtt ccattcataa tcccttgaat agactttata ttatttgcgc tcaaaccatc
     1621 tcttaaagca ttaataattg gaattccacc agcaactgct gcttcaaatt caaaaggtga
     1681 atcaccagca agttcttgaa gttcatatct atgatatgct agtagtgctt tattagcagt
     1741 aaccactgac ttaccatttt ctaaagctct ttttactatc tcataaggtt tttcaattcc
     1801 acccataagt tcaacaacta tatcaatgct atcatcttct aaaacttttg aaacatcatc
     1861 agtaagctct atttgcacat ctcttttttt attcaaattt gaaactacac caatagtagg
     1921 a
//
LOCUS       DAOQAN010000218         1917 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_89984_length_1917_cov_2.830290, whole genome shotgun sequence.
ACCESSION   DAOQAN010000218 DAOQAN010000000
VERSION     DAOQAN010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89984_length_1917_cov_2.830290"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1908)
                     /locus_tag="PKO10_04160"
     CDS             complement(<1..1908)
                     /locus_tag="PKO10_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014473726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNW66374.1"
                     /translation="MKFNNLLNTEKNFNIFKDRAVAGKFYVLEVEKELNFVARNSRDI
                     MLGNAYENNIKNLEKSRDNIIKSFDGLLQTSQNEAEKNVVLEVKKTVLDFIEDGYKKM
                     KSLETVERTPEVLAAMYQAYKKDATPLANKSRENFSKLIKEKEEDYKFQSELYNKQMK
                     GLKNIIIIESLVLVLIVILSLLLLSRDIIQSLNKFKKGLTSFFDFMNKHNSKVEYIDI
                     KNSDEFGAMAKVVNDNIKIIENRTLEDKKLIDDVFVVVNRVKHGYYSQLIKEDTSDEI
                     LSTLKNSINDMISSTKQNFIQVNNILEEYVKYNYREELKLDGIEKGGVFEALINNINQ
                     LRSAINDMLKKDKLNGLILDKSSNELLINVDKLNNSSNDAAARLEETAAALEEVTSSI
                     VNSNNKISEMSNIAKDVTNAASLGQELANKTVLSMDEINVQVNLVNDAISVIDQIAFQ
                     TNILSLNAAVEAATAGEAGKGFAVVAQEVRNLASRSAEAAKEIKSIVEEATKKANEGK
                     DIANEMIQGYKSLNDSINSTINLISDVEMSSKEQLSGIEQINDAVNQLDQQTQQNAAI
                     STQTNDIAITSDKIAKLIVEDANKKEFHGKNDIVAKSIEQNSKKFDNSLQIKKANLNK
                     ISTKENSSKVIQ"
BASE COUNT          576 a          319 c          192 g          830 t
ORIGIN      
        1 ttgaattact ttagaagaat tttcttttgt tgaaatttta tttaaattag cttttttaat
       61 ttgtaaacta ttatcaaatt tttttgaatt ttgttctata gattttgcga caatatcatt
      121 tttgccgtga aactctttct tatttgcatc ttcaactatc aattttgcaa ttttatctga
      181 agttattgct atatcatttg tttgtgttga tattgcagca ttttgttgag tttgttggtc
      241 aagttgattt actgcatcat ttatctgttc aattccagat aattgctctt tactcgacat
      301 ttcaacatct gaaattagat ttatcgttga gtttattgaa tcatttagac ttttatatcc
      361 ttgaatcatt tcatttgcaa tatctttacc ttcgtttgct ttttttgtag cttcttcaac
      421 tatagattta atctcttttg cagcttctgc acttctactt gcaagatttc ttacctcttg
      481 agcaacaaca gcaaatcctt ttccagcctc acctgcagtt gcagcttcaa cagcagcatt
      541 taaacttaat atatttgttt ggaaagctat ttgatcaatt actgaaattg catcatttac
      601 taaatttact tgaacattta tctcatccat agataaaaca gttttatttg ctaattcttg
      661 tcccaaagat gctgcatttg taacatcttt tgcaatattt gacatttcac ttattttatt
      721 attagagttt acaatactac ttgtaacctc ttctaaagca gctgctgtct cctctagtct
      781 tgctgctgca tcatttgaac tattatttaa tttatcaaca tttattaaca actcatttga
      841 agatttatcc aaaataagac catttagctt atctttttta agcatatcat ttatggcact
      901 tcttagttga tttatattat ttattaaagc ttcaaaaact ccaccttttt caattccatc
      961 taacttcaac tcttctctat agttatactt tacatactct tctaatatat tgtttacttg
     1021 tataaagttt tgttttgtag agcttatcat atcgtttata ctatttttta aagtagataa
     1081 aatctcatct gatgtatctt ctttaattaa ttgactataa tatccatgct ttactctatt
     1141 tacaacaaca aaaacatcat ctataagttt tttatcttct aaagttctat tttctataat
     1201 ttttatatta tcatttacta cttttgccat agctccgaat tcatcgctat ttttaatatc
     1261 tatatactca actttagagt tatgtttatt cataaagtca aaaaatgatg taagtccttt
     1321 tttaaactta tttaaggatt gaattatatc tcttgaaaga agtaataaag acaaaataac
     1381 tattaaaaca agaaccaaag actcaattat tataatattt ttcaaacctt tcatctgctt
     1441 attgtaaagt tctgattgaa atttataatc ttcctctttc tcttttataa gcttagaaaa
     1501 gttttctcta cttttgtttg caagaggtgt tgcatctttt ttataagctt gatacattgc
     1561 agctaaaact tctggagttc tttcaacagt ttcaagagat ttcatctttt tatatccatc
     1621 ttctataaaa tcaagtactg ttttttttac ttccaaaaca acattctttt cagcttcatt
     1681 ttgagaagtt tgtaataaac catcaaaaga ttttataata ttatctctac tcttttctag
     1741 atttttgata ttattttcgt aagcattccc aagcataata tctcttgaat ttctagctac
     1801 aaaatttaat tctttttcaa cttccaaaac ataaaattta ccagcaactg ccctatcttt
     1861 gaatatatta aagtttttct cagtatttaa aagattatta aacttcaaaa cacctgt
//
LOCUS       DAOQAN010000219         1916 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_90093_length_1916_cov_1.905427, whole genome shotgun sequence.
ACCESSION   DAOQAN010000219 DAOQAN010000000
VERSION     DAOQAN010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90093_length_1916_cov_1.905427"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..173)
                     /locus_tag="PKO10_04165"
     CDS             complement(<1..173)
                     /locus_tag="PKO10_04165"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdenumtransferase MoeA"
                     /protein_id="HNW66375.1"
                     /translation="MRDFISYNKSLEIINSIEFSKTPTQKLFITNAIGRVLAKDIVAD
                     HNSPEFPTSGMDG"
     gene            complement(176..373)
                     /locus_tag="PKO10_04170"
     CDS             complement(176..373)
                     /locus_tag="PKO10_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66376.1"
                     /translation="MTIEEEQIFIDKIKETLLPIVIYMKEEEIKKIILSVEEQNENLP
                     EGFGDMLFEQLIILKYNRLGK"
     gene            486..1268
                     /locus_tag="PKO10_04175"
     CDS             486..1268
                     /locus_tag="PKO10_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNW66377.1"
                     /translation="MIEISKKITTNVGRTNAQFGLIKENDRIMIGFSGGKDSLTLLHT
                     LNRMQKKAPFKFDFKAVTITYGMGEQIEFLSNHCKEHGIEHEIIDTQIFDLAGEKIRK
                     NSSFCSFFSRMRRGYLYTTALEQGYNKVALGHHLDDAMESYFMNLFYNGTMRTMPPIY
                     KASNGLEVIRPLIFCRERQLRAFADSNEIRVIGDEACPGLRFDVKMPHARATTKELLA
                     KLEEQNPKIFVSMKAAFSNIQLPTFFYKDLKDIKLNIEDENI"
     gene            1265..1759
                     /locus_tag="PKO10_04180"
     CDS             1265..1759
                     /locus_tag="PKO10_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014474199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HNW66378.1"
                     /translation="MRILVSSCLLGEDTRYDGLNSSIALNPKFRFSQKELFMDIMCEN
                     EIFSICPEVAGGLGIPRNKAEIKSHNKPFMVIDEKENDVTINFLIGAKKALDICLENS
                     IKVALFKSKSPSCGNIEVYDGTFSKKLVLEKGLTARLLEENGIKVFNENQLEDLKKFI
                     KENR"
BASE COUNT          714 a          237 c          299 g          666 t
ORIGIN      
        1 tatccatcca ttcccgaagt tggaaattca ggactattat gatcggctac tatatcttta
       61 gctaaaactc ttcctatggc atttgtgata aaaagttttt gagtaggagt tttactaaat
      121 tctatactat ttataatctc taaagatttg ttatatgaaa taaaatctct cataattatt
      181 tccccagtct attgtatttt agaatgatta attgttcaaa aagcatatct ccaaaacctt
      241 ctggtaggtt ttcattttgc tcttctacgc ttaaaataat tttttttatc tcttcttctt
      301 tcatatatat aactataggt agaagtgtct ctttgatttt atctataaat atttgttctt
      361 cttcgattgt cattttatat ctttttgatt aaattttaaa gagtcgatta tataatattt
      421 tattaaaata gtttattaaa actttttaga tataatccaa aaaaaataat ttaggatttt
      481 taaaattgat agaaataagt aaaaaaataa ctacaaatgt tggtagaaca aatgcccagt
      541 ttggcttgat taaagaaaat gatagaatta tgattggatt tagtggtggg aaagactctt
      601 tgactttatt acatacacta aatagaatgc aaaaaaaagc accttttaaa tttgatttta
      661 aagcagtaac aatcacttat ggaatgggag aacaaataga atttttaagt aatcattgca
      721 aagaacatgg gatagaacat gaaataatag atactcaaat atttgatctt gcaggtgaaa
      781 aaataagaaa gaactcttca ttttgttcat ttttttcaag aatgagaaga ggatatttgt
      841 acacaacagc tttagagcaa ggttacaata aagtagctct tggacatcat ttagacgatg
      901 caatggagtc ttattttatg aatctatttt ataatggaac tatgagaact atgccaccaa
      961 tatataaagc aagcaatggt ttagaagtaa ttcgtccact catattttgt agagagagac
     1021 aacttcgtgc ttttgcagat tcaaatgaga ttagagttat tggtgatgaa gcttgcccag
     1081 gtttacgatt tgatgttaaa atgcctcatg caagagccac tacaaaagag ttattagcaa
     1141 aacttgaaga gcaaaatcca aagatttttg tatctatgaa agctgcattt agtaatatac
     1201 aattacctac atttttttat aaagatttaa aagatataaa actaaatatt gaagatgaaa
     1261 atatatgaga atattggttt cgtcatgcct tttaggagaa gacacaagat atgatggatt
     1321 aaattctagt attgcattaa atcctaaatt tagattttca caaaaagaac tttttatgga
     1381 tattatgtgc gaaaatgaga ttttttcgat ctgccctgaa gttgcaggtg gtttaggaat
     1441 tccaagaaat aaagcagaga taaaaagcca taataaacct tttatggtaa ttgatgaaaa
     1501 agaaaatgat gtaactataa attttctaat tggtgcaaaa aaagctcttg atatatgttt
     1561 agaaaacagt ataaaagtag cactgtttaa atcaaaatct ccgtcatgtg gtaatattga
     1621 agtttatgat ggaacattta gtaaaaaact cgtacttgaa aaaggattaa cggcaagatt
     1681 gcttgaagaa aatggaatta aagtttttaa tgaaaaccaa ttagaagatt taaaaaagtt
     1741 tataaaagaa aatagatgaa agcataagtt atcaaactta tgcttttaaa tcaagtagtc
     1801 tttccatagc aacagcttga atctcaatac tttttgcatt tgcgctacat gctattattt
     1861 caggaatttg ctcaaccata gcagaagtta tatcttttgc agcatcttga aactgt
//
LOCUS       DAOQAN010000220         1915 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_90128_length_1915_cov_3.731183, whole genome shotgun sequence.
ACCESSION   DAOQAN010000220 DAOQAN010000000
VERSION     DAOQAN010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90128_length_1915_cov_3.731183"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(90..332)
                     /locus_tag="PKO10_04185"
     CDS             complement(90..332)
                     /locus_tag="PKO10_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66379.1"
                     /translation="MKTILVMILLSLLFSGCSQKVVIQKEFICYDLQDVTISDDVQIR
                     VHKDDLDLFEARNNELKSAISFYKTQNEKYNMECKK"
     gene            complement(289..573)
                     /locus_tag="PKO10_04190"
     CDS             complement(289..573)
                     /locus_tag="PKO10_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66380.1"
                     /translation="MNYLIAILSILVVVLFLFTKNLYNENKQLEENNKLIITAYESSI
                     ETLENKAKFEKEILIKKEPVIKASEKVKIENQKRGNIENNISDDFVITSF"
     gene            complement(570..845)
                     /locus_tag="PKO10_04195"
     CDS             complement(570..845)
                     /locus_tag="PKO10_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66381.1"
                     /translation="MLDYNLSLLILITSSGLAISFAFHHKMWRYCLSGFVFLILVSFI
                     LILRLFELVNLENWIAVHFYIISVGCIATIIYTISYKYKNYKKKSCK"
     gene            complement(838..1185)
                     /locus_tag="PKO10_04200"
     CDS             complement(838..1185)
                     /locus_tag="PKO10_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66382.1"
                     /translation="MIWYGGKMSDDFKTMLVVIGAFIFSVVSYLVEFWEDGLKLEKRH
                     TLYLFRAGVFGAGLGLIAFYGSDLILSYYFDKIIQVHEMIKAGITATVAGLSPWIFAT
                     ITRLIEKKGDSNA"
     gene            complement(1229..1786)
                     /locus_tag="PKO10_04205"
     CDS             complement(1229..1786)
                     /locus_tag="PKO10_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66383.1"
                     /translation="MPKETITKVQEQSGLGNILIATYKYLRDNNILKGVFKNPLVVVR
                     KTREAVQFYDKLILPSTTGTNKPIFTYKNISTDKEIRLCTFNVAVKSTKPVSLDMYKN
                     SNLTAASWIAFDGSTTIQKDISATSYSGGLLVGGVDVPETGGVYISQEDKPLFFTCLP
                     NETITIVLTAEHSSDIRMRIRLEEI"
BASE COUNT          612 a          311 c          225 g          767 t
ORIGIN      
        1 aaattgattt atttgattta tttttctttt tggctctatt ggtttaaaaa tttgtaataa
       61 aattataata atagctttac ttagagtttt catttcttac attccatatt gtacttttca
      121 ttttgtgtct tataaaaaga aatggcactt ttaagttcat tatttcttgc ttcaaataga
      181 tctaaatcat ctttatgaac tcttatttgt acatcatcac taatcgttac atcttgcaaa
      241 tcgtaacaaa taaactcttt ttgaataact accttttgcg aacaaccact aaaaagaagt
      301 gataacaaaa tcatcactaa tattgttttc aatatttccc cttttttgat tttctatttt
      361 tactttttca cttgctttta ttactggctc tttttttatt aaaatctctt tttcaaactt
      421 agctttattt tctaaagttt cgatactaga ctcataagct gtaataataa gtttgttatt
      481 ctcttctagt tgtttgtttt cgttatataa gtttttagtg aatagaaaca aaaccaccac
      541 taatattgat aaaatagcta ttaagtagtt catttgcaac tctttttttt gtaattttta
      601 tatttataag atattgtgta gattattgtt gcaatacatc cgactgaaat gatgtaaaaa
      661 tgaactgcta tccagttttc aaggtttact aattcaaaaa gtcttaatat caaaataaat
      721 gatactaaaa tcaaaaatac aaatccagat aagcagtatc tccacatttt atggtgaaat
      781 gcgaagctta tagccaatcc tgaacttgtt ataagtataa gtaaagataa attataatca
      841 agcattagaa tctccttttt tctcaattaa tctagtaatt gttgcaaata tccaaggact
      901 caaaccagca actgttgctg tgattccagc ctttatcatt tcatgtactt gtattatttt
      961 atcgaaataa tagcttagta ttaaatcgct tccataaaaa gcgataagtc caagtcctgc
     1021 tccaaaaact cctgctctaa ataaatatag agtgtgtctt ttttcaagct ttaaaccatc
     1081 ttcccagaat tctacaagat agcttactac tgaaaaaata aatgctccta ttactaccaa
     1141 cattgtttta aaatcatcac tcattttccc tccataccat atcattttcc ttgcttagtt
     1201 atttgtcttt attttatcaa aaaaactatt aaatctcttc tagtcttatt ctcattctta
     1261 tatcagatga gtgttctgct gttaaaacta ttgttatcgt ttcatttggc aaacaagtga
     1321 aaaaaagtgg tttgtcttct tgtgaaatat aaactccacc agtttctgga acatcaactc
     1381 caccaactaa aagtccacca gaatatgaag ttgctgaaat atctttttga atagttgttg
     1441 aaccatcaaa agcaatccaa gatgcagcag ttaaatttga atttttatac atatcaagag
     1501 atactggctt tgttgatttt actgcaacat tgaatgtgca tagccttatc tctttatctg
     1561 ttgagatgtt tttatatgta aagattggct tatttgtacc agtagttgat ggcaaaatta
     1621 gtttatcata aaactgtact gcttctcttg tttttcttac tactactaaa ggatttttaa
     1681 atacaccctt aaggatattg ttatccctaa gatatttata tgtagctata agtatatttc
     1741 caagtccact ttgttcttgt actttggtta tagtttcttt tggcatatta tattccgatt
     1801 tcagatgcta aagtattttc taaaattctt ttttcactta aagcagttcc tgctaaagtt
     1861 ctatagtttt tatcatttaa aatgattgca ttgcaaaaat cctctatagt tctac
//
LOCUS       DAOQAN010000221         1914 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_90197_length_1914_cov_2.860678, whole genome shotgun sequence.
ACCESSION   DAOQAN010000221 DAOQAN010000000
VERSION     DAOQAN010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90197_length_1914_cov_2.860678"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1188)
                     /locus_tag="PKO10_04210"
     CDS             complement(<1..1188)
                     /locus_tag="PKO10_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014472855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNW66384.1"
                     /translation="MNFEKYYLLDTNILLEDARNIFKLSDGAKNLIILSETVLDELDS
                     KKSGYDEINFQAREFARILENSEVLSSNKYEDFKIIRLKLINQDETIVDIISKDEYSI
                     NSKNISLNILNDRKILEVAKFAKNYYNKDIKFVSMDIMARTRAISLDIKSVALHGKDS
                     DKFNFDFIKNIDINFEDLENLDNQDIINFDKEYKVSDFSYCLKVKHSDQVILASIQNN
                     KIKILDEEEVRNQIITPLNKEQLFFSNAIINHFYNVLIVEAKAGSGKTLLALSGALKL
                     VKQKHFQKIIYIRNSIESLDKGEDVGYLPGLEEKFRIYNHPLMDSLEYIIRTEHKRRR
                     SKRDDISYTPLEDSEVSSRVEQMISNYGIETMWVGEMRGRTLSNSFIIIDEAQNMSNK
                     TMQM"
     gene            complement(1178..1702)
                     /gene="hemJ"
                     /locus_tag="PKO10_04215"
     CDS             complement(1178..1702)
                     /gene="hemJ"
                     /locus_tag="PKO10_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014467942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase HemJ"
                     /protein_id="HNW66385.1"
                     /translation="MEYYTWILSLHIIAVLSWMAMLFYLPRLFVYHVENSHKKEFVEV
                     VKIQEMKIYKYIGHPAMWITILSGISMIVLNPALLSQNWMIAKLFMLLLLIAYSFSLD
                     YYRKQLENNSCKRSGKFFRMYNEQPTLLAILIVTYVITKSFSILFTVIIVLLFAFISY
                     MIMKPKKVKKIDEF"
     gene            complement(1776..>1914)
                     /locus_tag="PKO10_04220"
     CDS             complement(1776..>1914)
                     /locus_tag="PKO10_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012011961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase RarA"
                     /protein_id="HNW66386.1"
                     /translation="YLYPHDFGGYVEQEYLKEDLKLYNSLDIGFEKTLSEWIRKIKNE
                     N"
BASE COUNT          657 a          301 c          181 g          775 t
ORIGIN      
        1 catctgcatt gttttatttg acatgttttg tgcttcatca attataataa atgagtttga
       61 aagcgttcga cctctcatct ctcctaccca catagtttca ataccataat ttgaaatcat
      121 ctgctcaact cttgagctta cttcactatc ttcaagtgga gtatatgaaa tatcatccct
      181 tttacttctt cttcttttgt gttcagttcg tataatatac tctaaactat ccattaaagg
      241 atgattatat attctaaatt tctcttcaag tcctggtaag taaccaacat cttcaccttt
      301 atccaaagac tcaatagagt ttcttatata tataattttt tgaaaatgtt tctgttttac
      361 aagttttaaa gcaccactta atgctaaaag tgtttttcca cttccagctt tagcttcaac
      421 tattaaaaca ttataaaaat gatttattat agcatttgag aaaaatagtt gctctttatt
      481 taaaggtgta attatttgat ttcttacctc ttcttcatca agaattttta ttttattgtt
      541 ttgaattgaa gccaaaataa cttgatctga atgtttaact tttaagcaat atgaaaaatc
      601 agaaacttta tattctttat caaaattgat tatatcttga ttatctaagt tttctaaatc
      661 ttcaaagttt atatcaatat tctttataaa atcgaaatta aatttatcac tatctttacc
      721 atgaagtgct acacttttta tatcaagaga tatagctctt gttcttgcca taatatccat
      781 agagacaaat tttatatctt tgttatagta attttttgca aactttgcaa cttcaagtat
      841 ttttctatca ttcaaaatat ttaaagatat atttttacta tttatagagt attcatcttt
      901 agagataata tcaactattg tttcatcttg gtttataagt tttaatctaa ttatcttaaa
      961 atcttcatat ttatttgagc ttaaaacttc tgaattctca agaattcttg caaactctct
     1021 tgcttgaaaa tttatctcat catatccact ttttttagag tctagttcat ctaaaactgt
     1081 ttcacttaag attattaagt tttttgcacc atcacttaat ttaaagatat ttctagcatc
     1141 ttcaagtagt atatttgtat ctaataggta atatttttca aaattcatct atttttttca
     1201 ccttttttgg tttcataatc atatatgata taaatgcaaa taataaaact atgattactg
     1261 taaaaagaat cgaaaaactt tttgtaataa cataagtaac aattaaaata gcaagtaatg
     1321 ttggttgctc attatacatt ctaaaaaatt ttccacttct tttgcaactg ttattttcta
     1381 gttgctttct ataataatct aaagaaaaag agtaagctat caaaagtaat aacataaata
     1441 gctttgcaat catccaattt tgacttagaa gtgctggatt taaaactatc atactaattc
     1501 cacttaaaat agttatccac atagctggat gacctatata cttgtatatt ttcatctctt
     1561 gtattttcac aacttcaaca aactcttttt tgtgactatt ttcaacatga tatacaaaaa
     1621 gcctaggaag ataaaatagc atcgccatcc aagataatac tgctattata tgcagactca
     1681 aaatccaagt ataatattcc atttaatttc tcctaatata ttaaactcta ttctatcaaa
     1741 attaacaaat attaaaatga aaatatatct atattttaat tttcattctt aatttttcta
     1801 atccattcac ttaaagtctt ttcaaatcca atatcaagtg aattataaag ttttaaatcc
     1861 tcttttaaat actcttgttc tacatatcca ccaaaatcat gaggataaag ataa
//
LOCUS       DAOQAN010000222         1907 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_90759_length_1907_cov_2.294276, whole genome shotgun sequence.
ACCESSION   DAOQAN010000222 DAOQAN010000000
VERSION     DAOQAN010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90759_length_1907_cov_2.294276"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="PKO10_04225"
     CDS             <1..508
                     /locus_tag="PKO10_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008338587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SelB C-terminal domain-containing protein"
                     /protein_id="HNW66387.1"
                     /translation="ALLSSSSINLRVPWASIEFINSALDDLVQNGFLVKEGQLYKNAN
                     IKEDITKELENIFLERLKSEDITPTAPYNIYDDLDIDRKLGDDILKSLTAKKDVIRLQ
                     HNLFIHSQSLNKIIKSMREIIKEDGFIEIFNFKQRFDLSRKYLVCYLDYLDNFSDIKK
                     VENRRVFS"
     gene            complement(521..697)
                     /locus_tag="PKO10_04230"
     CDS             complement(521..697)
                     /locus_tag="PKO10_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013135860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66388.1"
                     /translation="MFNWLFRKKEKRKREKTYVCKHCKLTCETCTTMFCYSCYSNPGN
                     PCPRCKKTNLMEKE"
     gene            complement(767..1597)
                     /locus_tag="PKO10_04235"
     CDS             complement(767..1597)
                     /locus_tag="PKO10_04235"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014474247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HNW66389.1"
                     /translation="MNIGIIGLGLMGGSLAKAVKRYGISKKVYGFTNSEKNKKEIEEL
                     GLVDELVDLETLKKVSDVIILSIPVDAIISMFPNFLDINEKTTIIDMGSTKEYIVKNI
                     PPKIRKNFIAAHPMTGTEKNGPKAAIDNLYEGKTVVLCDLEDNANMHVNKAFKIFQEI
                     GMRIVVMNSHEHDVNACYISHLPHLISFSLANTVMSHQNPKEIIALAAGGFKDMSRIA
                     KSSPRMWGDIFKQNRENMLESIKSFEDQMNEAKKMIEEERYEDLENWMKKANSLHEIL
                     "
     gene            1702..>1907
                     /locus_tag="PKO10_04240"
     CDS             1702..>1907
                     /locus_tag="PKO10_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66390.1"
                     /translation="MKNRITFLSLACATVLSASSIKSIEYLNVSKVSPQVLEETLGMR
                     AGDSLDSNKLNEALIKFYSYGYFD"
BASE COUNT          665 a          278 c          240 g          724 t
ORIGIN      
        1 cgccctactt tcaagctcat caataaattt aagagttcct tgggctagta ttgagtttat
       61 aaatagtgct ttagatgatt tggtacaaaa tggcttttta gtaaaagagg ggcaacttta
      121 taaaaacgca aatataaaag aggatattac aaaagagctt gaaaatatat ttttagaacg
      181 attaaaaagt gaagatataa cgccaacagc accttataat atctatgatg atttagatat
      241 tgatagaaaa cttggagatg atattttaaa atcacttaca gcaaaaaaag atgtaataag
      301 acttcaacac aatcttttta ttcatagcca aagtttaaat aagattataa aatctatgag
      361 agagattata aaagaagatg gttttataga gatttttaac tttaaacaga gatttgattt
      421 aagtagaaaa tatcttgttt gttatttgga ctatttagat aattttagtg atattaaaaa
      481 agttgaaaat agaagagttt tttcttaaag acaaaataga ctactctttt tccataagat
      541 ttgttttttt acacctagga caaggatttc caggatttga gtagcaagag tagcaaaaca
      601 ttgtagtaca agtttcacat gtaagcttac aatgtttgca aacataagtt ttttctcttt
      661 ttcttttctc tttttttcta aataaccaat taaacatagc tatccttttt aaataaaaag
      721 gattatattt aatctttttt acttataaga tgatatatat caaagcttat aatatttcat
      781 gtaaactatt tgcttttttc atccaatttt ctaaatcttc atacctctct tcttcaatca
      841 tttttttagc ttcattcatt tgatcttcaa aagatttaat actttctagc atattttctc
      901 tattttgctt aaaaatatct ccccacattc ttgggcttga ttttgcaatt ctactcatat
      961 ctttaaatcc tccagcagca agtgcaataa tctcttttgg attttgatga ctcataacag
     1021 tatttgctaa ggaaaatgat attaaatgag gtaaatgtga tatataacaa gcatttacat
     1081 cgtgttcatg actattcata accacaattc tcatacctat ttcttgaaaa atcttaaaag
     1141 ctttattaac atgcatattt gcattatctt ccaaatcaca taaaactaca gttttcccct
     1201 catataaatt atcaattgca gcttttggtc catttttctc tgttcctgtc attggatgtg
     1261 ctgctataaa attttttctt attttaggag gaatattttt tacaatatac tctttagttg
     1321 aacccatatc tataatagtt gttttctcat ttatatctaa aaaattagga aacatagaaa
     1381 taatagcatc aacaggaata gataaaataa taacgtctga aacttttttt aaagtttcca
     1441 aatcaacaag ctcatcaaca agtcctaact cttctatctc ttttttgttt ttttcactat
     1501 ttgtaaatcc ataaaccttt tttgaaatac catatctttt aacagccttt gccaaagaac
     1561 cacccataag tcctaaacca ataattccaa tgttcaaatt taaccttttt ttagtaattt
     1621 tgccaaaatt atagcttact ttagatttat ataaactata tttcggtata ttattgcttt
     1681 attttgacaa caaggacatt tgtgaaaaat cgaataactt ttttatcttt agcttgtgct
     1741 actgttttga gtgcaagtag catcaaatct attgaatact taaatgtaag taaagtatcg
     1801 ccacaagttt tagaagagac tttgggaatg agagctggtg atagcttaga tagtaacaag
     1861 ctaaatgaag cattaattaa attttatagc tatggttact ttgatga
//
LOCUS       DAOQAN010000223         1899 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_91413_length_1899_cov_1.911063, whole genome shotgun sequence.
ACCESSION   DAOQAN010000223 DAOQAN010000000
VERSION     DAOQAN010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91413_length_1899_cov_1.911063"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="PKO10_04245"
     CDS             complement(<1..186)
                     /locus_tag="PKO10_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66391.1"
                     /translation="MRIDNNLNAMLGASLQIQEQASNIAQVANTTADPQFQDAAKDIT
                     SAMVEQIPEIIAYSANAK"
     gene            complement(173..667)
                     /locus_tag="PKO10_04250"
     CDS             complement(173..667)
                     /locus_tag="PKO10_04250"
                     /inference="COORDINATES: protein motif:HMM:NF023540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metalloprotease CJM1_0395 family
                     protein"
                     /protein_id="HNW66392.1"
                     /translation="MEIQNDYLSASTIYSELALKRSELANIDKKELEKSTFEKSDSTN
                     PISKYDEKDYERVLNRFKSSDVETRNHEQLHASLGTTTTPIRYDYQMGPDGKLYAIGG
                     SVRFDTSIPKDKDEAIQKLDELQKASSSPDGLSRADSSIAQAANLNKMLIQSIQEGAN
                     YENR"
     gene            806..1363
                     /locus_tag="PKO10_04255"
     CDS             806..1363
                     /locus_tag="PKO10_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNW66393.1"
                     /translation="MNFLFNKHSHFNLANIVTFFNISCGIFAIYFLTHDEFMGAALFA
                     WLAGAFDIFDGKIARKYNLSTEFGIQLDSYADFLSFVIVPAMFIYFAIFDGKESFYNM
                     ALIAFVFIYYVISGLRRLIQFNINANEGEVEKYFTGIPTPLGAILLWVVYLIYLTDYV
                     NEYFVLGSIIIIGYLLNSKIKIKHL"
     gene            1360..>1899
                     /locus_tag="PKO10_04260"
     CDS             1360..>1899
                     /locus_tag="PKO10_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HNW66394.1"
                     /translation="MKNEVITIDLGSNSFRVLKYDCLNFKIIDEYHQVVGLADGLINS
                     GLISFEALNRVIDAINIVKDKLSFKPENAVCVTTAAMRKAKNSQEALDFIEKNSGAKF
                     KIIDSNEEARLTLLAIKYALKREKIDTQNFILLDIGGGSTELIVNFENNFYIKSFDFG
                     IVTMTQKSLKDGKLFEDLEN"
BASE COUNT          649 a          227 c          276 g          747 t
ORIGIN      
        1 ttttgcattt gcactatatg ctattatttc gggaatttgc tcaaccatag cagaagttat
       61 atcttttgca gcatcttgaa actgtgggtc agctgttgta tttgcaactt gagcaatatt
      121 agatgcttgt tcttgaattt gtaaactagc tcctaacata gcatttagat tattatctat
      181 tctcataatt agctccttct tgtatagatt gtattaacat tttatttaaa tttgcagctt
      241 gtgcaataga actatcagct ctacttaaac catcaggaga agatgaagct ttttgtagtt
      301 catctaattt ttgtatcgct tcatctttat cttttggaat agatgtatca aatcttacac
      361 ttcctccaat tgcatatagc tttccatctg gtcccatttg ataatcatat ctaataggag
      421 ttgtagtagt tcctaaacta gcatgtaact gctcatgatt tctagtttca acatcactag
      481 atttaaatct atttaaaact ctctcataat ctttttcatc atatttagaa ataggatttg
      541 tactatcact tttttcaaaa gttgattttt ccaactcttt tttatcaata tttgctaact
      601 cacttctttt taaagctaat tcagaatata ttgtggaagc acttaaataa tcgttttgta
      661 tttccatttt tttacctata atataattta atgctattgt aacataaaat attaaagaag
      721 attatataag agaaattttt atataaacta tatattgagc taatatatag taaaatttca
      781 gctatttaat tttaaaagag ggaaaatgaa ttttttattc aataaacata gtcacttcaa
      841 tcttgcaaat attgtaactt tttttaatat ctcttgtgga atttttgcaa tatatttttt
      901 aactcatgat gagtttatgg gggctgctct ttttgcttgg ctagctggtg catttgatat
      961 atttgatggt aaaatagcaa gaaaatataa tctttcaaca gagtttggaa ttcaacttga
     1021 ctcttatgct gattttttat cttttgtaat tgttcctgca atgtttatat attttgctat
     1081 ttttgatgga aaagagtcat tttacaatat ggctttaata gcgtttgttt ttatttatta
     1141 tgttatttct ggtcttagaa gacttattca atttaatata aatgcaaatg aaggtgaagt
     1201 tgaaaaatat tttactggta ttccaacacc cttaggagca attcttcttt gggtagtata
     1261 tttaatatac ttaacagatt atgtaaatga gtattttgtc ttaggttcta taataattat
     1321 tggatattta ttaaattcaa aaataaagat caaacattta tgaaaaatga agtcataaca
     1381 attgatttag gttcaaactc ttttagagtg ttaaaatatg actgtttgaa ttttaaaata
     1441 atagatgaat atcatcaagt tgttggactt gctgatggtt taataaatag tggattaata
     1501 agttttgaag ctttaaatag agtaatagat gcaataaata ttgtaaaaga taagttgagt
     1561 tttaaaccag aaaatgcagt ttgtgtaaca actgctgcta tgagaaaagc aaaaaactca
     1621 caagaagcat tagattttat tgaaaaaaat agtggtgcaa aatttaaaat aatagattca
     1681 aatgaggaag caagacttac tttacttgct ataaaatatg ctttaaaacg agagaaaata
     1741 gatacacaaa actttatatt acttgatatt ggtggtggtt caacagagct tatagttaat
     1801 tttgagaata atttttatat aaaaagtttt gattttggaa tagttacaat gacacaaaaa
     1861 tctttaaaag atggaaagtt attcgaagat ttagaaaat
//
LOCUS       DAOQAN010000224         1898 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_91463_length_1898_cov_2.329897, whole genome shotgun sequence.
ACCESSION   DAOQAN010000224 DAOQAN010000000
VERSION     DAOQAN010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91463_length_1898_cov_2.329897"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1153)
                     /gene="mtaB"
                     /locus_tag="PKO10_04265"
     CDS             complement(<1..1153)
                     /gene="mtaB"
                     /locus_tag="PKO10_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HNW66395.1"
                     /translation="MNFSEHRPKVYFKTFGCRTNIFDTQVMISNLKDFDVTNNEKEAN
                     VVIINSCTVTNSADTTARSYINSLKKLENSPKVIFTGCGTRTKGEKLFADDKIDGLFG
                     SSEKENINELLKLDDKFYKLGDLKSLDTTVVEEFVGKSRAFIKIQEGCDFRCSYCIIP
                     HVRGDARSYEESIILNQVQTLANNGFSEFILTGTNVGSYGKKMHTSLAKLLKKMPLIK
                     GVKRIRMGSIEPIQIDDEFKELINEPFMAKHLHIALQHTSKEMLKIMNRRNKVLSDLE
                     LFEFLSQNGYALGTDFIVGHPGETQELWNEAMKNLHNFPLTHIHAFTYSKRDGTPSAS
                     MKDIVKGDIAKDRYIELVEIIKQKNYEFRQNIKNNKVKLEVLVEQEKNGK"
     gene            complement(1166..>1898)
                     /locus_tag="PKO10_04270"
     CDS             complement(1166..>1898)
                     /locus_tag="PKO10_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HNW66396.1"
                     /translation="SYLVTILGFASAGIAIALKDWFMSIFGWLVIVTSGFIQVGDRIR
                     VTKGEVETVGDVLDISLFKITIKEDVTLVSYMKNRRAGRIFFVPNNYIFSELISNYSH
                     SELKTVWDGIDITLTFDSNFKKAQKIIRDILKHYSKGYSDITRKQLSKMRNKYQLRAT
                     GVEPRVFTLLEPHGMVISGWYLTNSFAALVLRSTICAEILEALMKEDDIHIAYPTQQI
                     NINKTSNPYGPASKMPKKEFEVDDN"
BASE COUNT          631 a          335 c          199 g          733 t
ORIGIN      
        1 acttaccatt tttttcttgt tcaactaaaa cctctagttt aactttatta ttctttatat
       61 tttgtctaaa ttcgtagttc ttttgtttaa ttatctctac aagttcaata tatctatctt
      121 ttgcaatatc accttttaca atatctttca tagaagcact tggtgttcca tctcttttag
      181 aataagtaaa agcatgaata tgagtgagag gaaaattatg caaatttttc atagcttcat
      241 tccaaagctc ttgagtttct cctggatgac caactataaa atcagttcct aaagcataac
      301 cattttggct taaaaactca aaaagctcta aatcacttaa aactttattt cttctattca
      361 taatttttaa catctctttt gaagtgtgtt gaagtgctat atgaagatgt tttgccataa
      421 aaggctcatt tataagctct ttaaattcat catcaatttg aataggttca atacttccca
      481 ttctaattct cttaacacct tttataagtg gcattttttt aagaagtttt gctaaagaag
      541 tatgcatttt tttaccataa cttcctacat ttgtacctgt taaaataaac tcactaaaac
      601 cattattagc caaagtttgc acttgattta aaataatact ctcttcataa cttctagcat
      661 cacctcttac atgtgggata atacaataag aacatctaaa atcacacccc tcttgtattt
      721 ttataaatgc cctacttttt ccaacaaact cttcaacaac tgttgtatca agacttttta
      781 aatcaccaag tttatagaat ttatcatcta attttaaaag ttcattaata ttctcttttt
      841 cacttgaacc aaaaagtcca tcaattttat catctgcaaa aagcttctca ccctttgttc
      901 ttgttccaca acctgtaaat attacttttg gagagttttc aagttttttt aaactattta
      961 tatagcttct agctgttgta tctgcactat ttgtaactgt acaagagttt attattacaa
     1021 catttgcttc tttttcatta tttgttacat caaaatcttt taggttacta atcatcactt
     1081 gtgtatcaaa aatatttgtt ctacaaccaa aagtcttaaa ataaaccttt ggtctatgtt
     1141 cactaaaatt cataaaatat ttatcctaat tatcatctac ttcaaactct ttttttggca
     1201 tttttgatgc tggaccataa ggatttgaag ttttatttat atttatttgc tgagttggat
     1261 aagctatatg tatatcatcc tctttcatta aagcttcaag tatttcagca caaatagtac
     1321 ttcttaaaac aagtgcagca aaagagtttg taagatacca accagaaata accattccat
     1381 gaggttctaa tagagtaaat actcttggtt caactccagt tgctctaagt tgatacttat
     1441 ttctcatttt tgaaagttgt tttcttgtaa tatcactata accttttgaa tagtgtttca
     1501 aaatatctct aataattttt tgagcctttt taaaatttga atcaaaagtt aaagttatat
     1561 caattccatc ccaaactgtt tttagttcgc tatgactata atttgaaatt agttcagaga
     1621 atatatagtt atttggtaca aagaaaattc ttccagctct tctatttttc atatatgaaa
     1681 caagcgtaac atcctctttt atagtaattt taaataatga aatatctaaa acatctccaa
     1741 cagtttcaac ctctcctttt gtaacacgaa ttctatctcc aacttgaata aaaccagaag
     1801 ttacaataac taaccaacca aaaatagaca taaaccaatc ttttaatgca atagcaatac
     1861 cagcagatgc aaatcctaaa attgttacaa gatatgaa
//
LOCUS       DAOQAN010000225         1891 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_91932_length_1891_cov_3.694444, whole genome shotgun sequence.
ACCESSION   DAOQAN010000225 DAOQAN010000000
VERSION     DAOQAN010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91932_length_1891_cov_3.694444"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1357
                     /locus_tag="PKO10_04275"
     CDS             <1..1357
                     /locus_tag="PKO10_04275"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HNW66397.1"
                     /translation="LNTLLFATTFLALFFIILYIRELKNEKNIRKKIIDNAKKEVEER
                     LYKDELTGLKNRKSLEDSIKGKDSVVAILLDVDAFEDLNELYGFINAEEVLKELSNIL
                     KEFEKTHDVVAYRLSGDIFALTNISNIPFEDIFILIEELNKTFLNRKIFVDKLNVDIV
                     VSMTMGISIFQEEPILTAAMALKKAKSLNQSYFVYNNELDSKELIIQSLYWREKIKNA
                     VSENKIIPFYQPIVNRKKEVIKYESLMRIRDFDSNNEAIILTPDKFLGISFKTKQYLE
                     LSRIIISKSLDNLLKTQKNITINLSFKDILNYEFIDYLDNVLEKLKFEDRNRLVFEIL
                     ESENLSDYDFLEEFVLKYKKLGVKIAIDDFGSGYSNFIRIIRLKPDYLKIDGSLIKNI
                     DKDNNSYEIVKSIIAFSKTLNIKTIAEYVHSEEIFNLLLELDVDEFQGYYFGKPDEDF
                     S"
     gene            1417..1641
                     /locus_tag="PKO10_04280"
     CDS             1417..1641
                     /locus_tag="PKO10_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66398.1"
                     /translation="MSTITKIEELDLAGTKKGKIVISNVTEPYGANTADIVSIAIALN
                     GEDVQWKAHIPYENIDGLIEALNKAKNLKK"
     gene            1692..>1891
                     /locus_tag="PKO10_04285"
     CDS             1692..>1891
                     /locus_tag="PKO10_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008336498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66399.1"
                     /translation="MSQLKTIGRVETISILDLELFDLDAKIDTGAYSNSLHCDDIFVD
                     EDNFALVRKLTEIIFVQALRIH"
BASE COUNT          767 a          172 c          296 g          656 t
ORIGIN      
        1 gctaaatact ctcttatttg caacaacatt tttagctctt ttttttataa ttttatatat
       61 aagagaacta aaaaacgaaa aaaatattcg taagaaaatt attgataatg caaaaaaaga
      121 ggtagaagag agactttata aagatgagct tacaggtctt aaaaatagaa aatctcttga
      181 agatagtata aaaggaaaag attctgttgt tgctatttta cttgatgtag atgcttttga
      241 agatttaaat gaactttatg gatttataaa tgctgaagag gttttaaaag agctatcaaa
      301 tattttaaaa gagtttgaaa aaactcatga tgtagttgca tatagattaa gtggtgatat
      361 ttttgctctt acaaatataa gtaatatacc atttgaagat atttttattt taattgaaga
      421 gttaaataaa acatttttaa atcgaaaaat ttttgttgat aaattgaatg ttgatattgt
      481 tgtatctatg actatgggaa tttcaatttt tcaagaagag cccatattaa ctgcagctat
      541 ggctttaaaa aaagcaaaat cattaaatca aagttatttt gtttataata atgaacttga
      601 ttcaaaagag cttataatac agtcacttta ttggcgtgaa aagataaaaa atgcagttag
      661 tgaaaataaa atcattcctt tttatcaacc aatagtaaat agaaaaaaag aagtaataaa
      721 atatgaatct ttaatgagaa taagagattt tgattctaat aatgaagcta ttattcttac
      781 tcctgataaa tttttaggaa tttcatttaa gacaaaacag tatttggaac tttcaagaat
      841 tattatctca aaaagtttag ataatctttt gaaaactcaa aaaaatataa ctataaattt
      901 gagtttcaaa gatatcttaa actatgaatt tatagattat ttagataatg ttttggaaaa
      961 attaaagttt gaagatagaa ataggcttgt ttttgagatt ttagagagtg aaaacttaag
     1021 tgattatgat tttttagaag agtttgtttt aaaatataaa aaattaggtg taaaaatagc
     1081 catagatgat tttggaagtg gttattcaaa ttttattaga ataataagac ttaaaccaga
     1141 ttatttgaaa attgatggaa gtttaataaa aaatatagat aaagataaca actcttatga
     1201 gattgtaaaa tcaataattg cttttagtaa aactttaaat attaaaacta ttgctgaata
     1261 tgtacatagc gaagagatat ttaatctttt acttgaactt gatgtagatg aatttcaagg
     1321 ttactatttt ggaaaaccag atgaggattt ttcataaatt aatcttataa aaagtatttt
     1381 ttggataaat tttatcaaaa tataaaagga gaaaagatga gtactattac aaaaatagaa
     1441 gagcttgatt tagcaggtac aaaaaaagga aaaattgtta tttcaaatgt aactgagcca
     1501 tatggtgcaa atacagctga tatagtaagt attgcgattg cactaaacgg tgaagatgtt
     1561 cagtggaaag cacatattcc ttatgaaaat atagatggat taatagaagc tttaaataaa
     1621 gcaaaaaatt taaaaaaata gagctaaaaa aatatattgt gatataattc cttctatatt
     1681 tagggggttt tatgtcgcaa ctaaaaacta ttggaagagt agaaactata tctattttgg
     1741 atttagagct ttttgattta gatgcaaaaa ttgatacagg agcttactca aattctcttc
     1801 actgtgatga tatatttgtg gacgaagata acttcgcgtt agtcagaaag ctgaccgaaa
     1861 ttatcttcgt gcaagcgtta agaatccaca t
//
LOCUS       DAOQAN010000226         1889 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_92122_length_1889_cov_2.352781, whole genome shotgun sequence.
ACCESSION   DAOQAN010000226 DAOQAN010000000
VERSION     DAOQAN010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92122_length_1889_cov_2.352781"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..861)
                     /locus_tag="PKO10_04290"
     CDS             complement(<1..861)
                     /locus_tag="PKO10_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014475482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNW66400.1"
                     /translation="MRKSINHLYLIILISILSSVAPMGVDTYLPSIPEIAKYFDVNIH
                     KVELSLSIFLIGFAIGQIFGGPISDRYGRRVGSIVGLLGYALFSFLIIFSSSIYELWI
                     YRFLEAFFGGITVVNATAAVRDRFSGQEAAKVFSLIGMVRSLAPLLAPVFGAAIIHFF
                     PWEGIFVFLTIYALIVAFFIYKDLPESFTYTKQNIVESYKLVLTHKKAMKAMLVLAIS
                     FGAFFIIIAKTSYIYIEYFGIKTDYFPLFFGINFIILIAMIKVNVNLLKTYEAKNIAK
                     TYIEEPNATAG"
     gene            complement(987..1424)
                     /locus_tag="PKO10_04295"
     CDS             complement(987..1424)
                     /locus_tag="PKO10_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HNW66401.1"
                     /translation="MSKVIKQLKQIQADAHALYIKVHNYHWNVKGMDFHPVHAYTEGL
                     YNEMSVLFDDMAERTIILGDKPYLTIEELSEATKIETEKGDSFKSREIVEKITADFEY
                     LLKSFKKLSTAASEEDDKGTEAFADEKVAKLEKDLWMLNSMQK"
     gene            complement(1486..>1889)
                     /locus_tag="PKO10_04300"
     CDS             complement(1486..>1889)
                     /locus_tag="PKO10_04300"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNW66402.1"
                     /translation="SKTLPYDGIYELLDELKKLDINLAVLSNKPHEFTVSYVNHFFKN
                     YNFKEIHGQKKDVPKKPDPKAALDIVKCLDSSCENTYFIGDTKIDMQTAKSANMTAIG
                     VLWGFRDEKELRDFGADFIVSNPLEILKIIK"
BASE COUNT          681 a          292 c          222 g          694 t
ORIGIN      
        1 tcctgctgtt gcattcggtt cttctatata ggtttttgca atatttttag cttcataagt
       61 ttttagaaga tttacattta cttttatcat tgctattaaa ataataaaat ttataccaaa
      121 gaatagtggg aaatagtctg tttttatacc aaaatattca atataaatat acgatgtttt
      181 tgcaataata ataaaaaaag caccaaaact aatagcaagt accaacatag ctttcatggc
      241 ttttttatga gttaaaacaa gtttgtatga ttcaacaata ttttgttttg tatatgtaaa
      301 actctctggt aaatctttat aaataaaaaa tgcaacaatt agtgcataaa ttgttaaaaa
      361 gacaaaaata ccttcccatg ggaaaaagtg aattatagca gcaccaaata caggagccaa
      421 aagaggagcc aaacttctaa ccattccaat aagtgaaaaa acttttgctg cttcttgtcc
      481 gctaaatcta tctcttacag cagcagttgc attaacaact gtaattccac caaaaaatgc
      541 ttctaaaaat ctataaatcc ataattcata aatagatgaa ctaaaaatga ttaagaaact
      601 aaaaagtgca tatcctaaaa gcccaacaat agaaccaact cttcttccat atctatcaga
      661 aattggacca ccaaaaattt gtccaatagc aaatcctatt aaaaatatag ataaagatag
      721 ctctactttg tgaatattta catcaaaata ttttgcaatt tctggaattg atggtaaata
      781 tgtatcaact cccattggag ctactgaaga taaaatagat ataagaataa ttaaatatag
      841 atgattaata gattttcgca taaaaactca tttgtaaaat attataaatt tttttgattt
      901 taaagattag aaattaaaaa attgatagta aaattttagt taaaagagga taaaaatcct
      961 cttttataga ttttataggc ttgtgcctat ttttgcatgc tatttaacat ccataaatct
     1021 ttttcaagtt ttgcaacttt ttcatctgca aatgcttcag ttcctttgtc atcttcttca
     1081 ctagcagcag tacttagttt tttgaaagat tttaaaaggt attcaaaatc agcagttatt
     1141 ttttctacta tctctcttga tttaaagcta tcaccttttt cagtctctat ttttgtagcc
     1201 tcacttaact cttcaatagt taaatatggt ttatcaccta agataattgt tctttctgcc
     1261 atatcatcaa aaagaactga catctcattg taaagtcctt ctgtataagc atgaactggg
     1321 tgaaaatcca taccttttac attccagtga taattatgaa cttttatata caaagcatgt
     1381 gcatctgctt gaatttgttt taattgttta attacttttg acattttata actccttgaa
     1441 attttgtaaa accattatag tatcaaaaaa taagcttgtc aagatttatt taataatttt
     1501 taagatctca agaggatttg aaactataaa atcagcacca aaatctctta actctttctc
     1561 atctctaaat ccccataaaa caccaattgc agtcatattt gcactttttg ctgtttgcat
     1621 atctattttt gtatctccta taaaataggt attttcacaa gaactatcta aacattttac
     1681 aatatcaagt gcagctttag gatctggttt ttttggaaca tctttttttt gtccatgaat
     1741 ctctttaaag ttataatttt tgaaaaaatg gtttacatat gaaactgtaa attcatgagg
     1801 tttatttgat aaaactgcca aattaatatc caactttttt aactcatcta ataattcata
     1861 aattccatca taaggtagag tttttgagt
//
LOCUS       DAOQAN010000227         1889 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_92141_length_1889_cov_1.995638, whole genome shotgun sequence.
ACCESSION   DAOQAN010000227 DAOQAN010000000
VERSION     DAOQAN010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92141_length_1889_cov_1.995638"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            217..1485
                     /gene="murA"
                     /locus_tag="PKO10_04305"
     CDS             217..1485
                     /gene="murA"
                     /locus_tag="PKO10_04305"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014469220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HNW66403.1"
                     /translation="MDYLKIIGNQKLSGEIYISGAKNAALPLIAATILAKNETIINNL
                     PNVADINTFLKLIKMLGGSFTQDKNRAIIDTSTITNTTATYDIVKTMRASILVLGPLL
                     ARFGHCEVSLPGGCAIGQRPVDLHLKAMEAMGAKIEIRQGYIKATAPNGLKGAKIVFD
                     KVTVGGTENTVMAAALASGVTTIINAAKEPEIVQLCEVIASAGVKIEGIGTSKIIIEG
                     TGGELLDIKPFSVIPDRIEAGTYMCAAAITNQKLTIKNIVPAHLEAVISKLEEMNFEI
                     GIKEDELTIFPTSKILPVNIITTEYPGFPTDMQAQFMALATQAEGTSTIDERLFENRF
                     MHVSELLRMGADIQLNGNIATIIGEKGNLSGTDVMATDLRASSALVLAALVAQGETNI
                     HRIYHLDRGYEDLEGKFLKIGANIQRCKEK"
     gene            1489..>1889
                     /locus_tag="PKO10_04310"
     CDS             1489..>1889
                     /locus_tag="PKO10_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66404.1"
                     /translation="MLHNIKKLFESKEEEKKELKFNKFQLFLDLVNSYLKREYPNITF
                     DLSLYENNEEEDNLKVQKAMLIYEVIRQFADFKWERRTQKSVKKDLIWSGYGINSQPN
                     KKIPQDFLRRKELVFFRDEGICNRCGNSMDK"
BASE COUNT          739 a          226 c          337 g          587 t
ORIGIN      
        1 aaatactatt tcaatagtgc ttaacttatc gctaagttct tttgcagcaa ctcccattaa
       61 agcaaataaa aaagaggcaa taatcatata ttttatagct ttatttacat tttcttccaa
      121 tccaaaatcc ttttaaaagc aaaagtattg caaaatttgg gttaaagcaa tatttcgata
      181 aaatcttaac cattttaaac attatttagg aaatttatgg attatttaaa aatcatagga
      241 aatcaaaaac taagtggaga aatatatatt tctggagcaa aaaatgctgc acttcctcta
      301 atagcagcta caattttagc aaaaaatgaa actatcataa acaatcttcc aaatgtggct
      361 gatataaata cttttttaaa gttaataaaa atgctaggtg gaagttttac tcaagataaa
      421 aatagggcaa taatagatac aagcactatt acaaatacaa cagcaactta tgatattgta
      481 aaaactatga gagcttctat tttggtttta ggtccactat tagctagatt tggacattgt
      541 gaggtttctc ttccaggtgg ttgtgcaata ggtcagcgac ctgttgattt gcatttaaaa
      601 gctatggaag ctatgggagc aaagattgaa attcgccaag gttatataaa agcaactgca
      661 ccaaatggat taaaaggtgc aaaaattgta tttgataaag ttacagttgg tggaactgaa
      721 aatacagtta tggcagctgc tttagcttct ggagttacaa ctataataaa cgcagcaaaa
      781 gagccagaga ttgttcaatt atgtgaagta atagcaagtg ctggagtaaa aatagaagga
      841 attggaactt ctaaaattat aattgaagga acaggtggag aacttcttga tattaaacct
      901 tttagcgtaa ttccagatag aatagaagct ggaacttata tgtgtgctgc tgctattaca
      961 aatcaaaaat taactataaa aaatattgtt ccagctcatc ttgaagctgt tatttcaaag
     1021 ttagaagaga tgaattttga gataggtata aaagaagatg aacttacaat ttttccaaca
     1081 tcaaaaattt tacctgtaaa tattataaca acagaatatc caggatttcc aacagatatg
     1141 caagctcagt ttatggcact tgcaactcaa gctgaaggga ctagcacgat agatgagaga
     1201 ctttttgaaa atagatttat gcatgttagt gaacttttaa gaatgggtgc agatattcag
     1261 ttaaatggaa acatagcaac aattattgga gaaaaaggta atttaagtgg aacagatgtg
     1321 atggcaacag atttaagagc atcttctgct ttagttcttg cagcacttgt tgctcaagga
     1381 gagacaaata ttcatagaat ttatcatctt gatagaggat atgaagattt agaaggaaaa
     1441 tttttgaaaa ttggtgcaaa tattcaaagg tgtaaagaaa agtaatttat gcttcataat
     1501 attaaaaaac tatttgaatc taaagaagaa gagaaaaaag agttgaaatt taataaattt
     1561 cagctctttt tagatttggt taatagttat ttaaaaagag agtatccaaa tattactttt
     1621 gatttatctt tgtatgaaaa taatgaagaa gaagataatt taaaagttca aaaagctatg
     1681 cttatttatg aagttataag acaatttgca gattttaaat gggaaaggag aacccaaaaa
     1741 agtgtaaaaa aggatcttat ttggagtggt tatggaataa actctcaacc aaataaaaag
     1801 ataccacaag attttttaag aaggaaagag ttagtttttt ttagagatga gggaatttgt
     1861 aatagatgcg gaaattctat ggacaaaat
//
LOCUS       DAOQAN010000228         1887 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_92327_length_1887_cov_1.770742, whole genome shotgun sequence.
ACCESSION   DAOQAN010000228 DAOQAN010000000
VERSION     DAOQAN010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92327_length_1887_cov_1.770742"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(16..1047)
                     /locus_tag="PKO10_04315"
     CDS             complement(16..1047)
                     /locus_tag="PKO10_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNW66405.1"
                     /translation="MRELKILAVVVVLTLITYWGVEPFAHSQMHPHVDPVNYNFEESD
                     RTTSKEAVEKAQKALAEAEKKADEKAIKGAKADLEAALTFEKTINAFWDANKAAITAT
                     PNVANGEALVTANCTACHSIESKGFPKLMDDASTAGAYGVATPDLGSAGKLYSKDYLI
                     AFINNPALGSKVSHKFTDGRAHPMPSYELMNTPQEIADMVAYLQSIAPKEMTNKEVFV
                     DACQRCHGLKYADMQKGTMGAFSPDADITKYMGKLPPDLSQYIISRGPDYLGKFINDP
                     QKLLEGTAMPRVGLNQESQEQVIKYLEEVGASKKAEREELGPKFLIYLVIFAIFAFLW
                     KASKWRDVH"
     gene            complement(1051..>1887)
                     /locus_tag="PKO10_04320"
     CDS             complement(1051..>1887)
                     /locus_tag="PKO10_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002856614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HNW66406.1"
                     /translation="SAAGFSGYMLPWGQMSYWAAMVITNLFGGVPVIGDALVVWIRGD
                     FNVADATLTRFFMLHVFLLPIAIMGLIGLHFYTLRIPHVNNQSSEDIDFDAEAEKYLS
                     GNKKESKVIPFWPVFISKDLAVLGVFLIFYFYLVFFHYSFAMDPVNFDPADPMVTPAH
                     IYPEWYFLWSYEVLRGFFFDIAGIKAFDIGLIAFAFANGIFFVLPWLDRDNKILPAHK
                     RPLFFIWFWLLVVDLIVLTVYGKLPPTGANAWVGFFAAVAFILLFVLLPVITKLDQKR
                     RG"
BASE COUNT          632 a          353 c          287 g          615 t
ORIGIN      
        1 ttaaaagaga tgttcttagt gaacatctct ccatttactt gctttccata ggaatgcaaa
       61 gattgcgaaa attactagat agattaggaa tttaggacct aactcttctc tttctgcttt
      121 tttagaagca cctacttctt caaggtattt tataacttgt tcttgtgact cttgatttaa
      181 accaactcta ggcattgcag taccttcaag tagtttttga gggtcattta taaatttacc
      241 taaatagtca ggacctctac taataatata ttgagataaa tctggaggaa gttttcccat
      301 atattttgta atatcagcat caggtgagaa tgcacccatt gttccttttt gcatatcagc
      361 atattttaaa ccatgacatc tttgacatgc atcaacaaac acctctttat tcgtcatctc
      421 ttttggagca attgattgta aatatgcaac catatcagca atttcttgtg gagtattcat
      481 tagctcatag ctaggcattg gatgagctct tccatctgta aatttatgag aaacttttga
      541 cccaagtgct ggattattaa taaaagcaat taaataatct ttagaataaa gcttacctgc
      601 gcttcctaaa tcaggagttg caacaccgta agcaccagct gtagatgcat catccattaa
      661 ttttggaaaa ccttttgatt caatactatg acaagctgta cagtttgccg ttactaaagc
      721 ttcaccattt gcaacattag gagttgcagt tattgcagct ttattagcat cccaaaaagc
      781 atttatagtt ttttcaaaag ttaaagctgc ttctaaatca gcttttgctc ctttaatagc
      841 tttttcatct gcttttttct cagcttcagc taaagctttt tgtgcttttt caactgcttc
      901 ttttgaagta gttctatcag attcttcaaa gttatagttt acaggatcaa catgcggatg
      961 catttgtgag tgagcaaaag gctcaactcc ccagtaagta ataagagtta atactactac
     1021 tactgctaaa atttttaatt ctctcatctt ttaccctctt cttttttgat caagttttgt
     1081 aataactgga agtagaacaa atagtagtat aaatgctact gctgcaaaga aacctaccca
     1141 agcatttgca cccgttggag gaagttttcc ataaacagtt aatacgatta aatctacaac
     1201 taatagccag aaccagataa aaaatagtgg tcttttatga gctggtaaaa ttttattatc
     1261 tctatctagc catggtagaa caaagaagat accatttgca aatgcaaacg caattaaacc
     1321 aatatcaaat gctttaattc cagcaatatc aaagaagaac cctcttaaaa cttcatatga
     1381 ccataagaaa taccattctg gatagatatg agctggagtt accataggat cagctggatc
     1441 aaagttaact gggtccatag caaaactata gtggaagaac actaaataga agtagaatat
     1501 taaaaacact ccaagaactg ctaaatcttt tgagataaat acaggccaga aaggaataac
     1561 ttttgactct tttttatttc cacttaaata tttttcagct tcagcatcaa aatcaatatc
     1621 ttcagaagat tgattattta cgtgaggaat tcttaatgta tagaagtgta aacctattaa
     1681 tcccataata gctattggta ataagaatac atgtaacata aagaatcttg taagtgttgc
     1741 atcagcaaca ttaaaatctc ctctaatcca aacaactagt gcatctccaa taacaggaac
     1801 tccaccaaat aagtttgtga taaccattgc agcccagtaa gacatttgtc cccaaggaag
     1861 catatatcca gagaaaccag cagcact
//
LOCUS       DAOQAN010000229         1883 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_92595_length_1883_cov_2.888950, whole genome shotgun sequence.
ACCESSION   DAOQAN010000229 DAOQAN010000000
VERSION     DAOQAN010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92595_length_1883_cov_2.888950"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /gene="hemC"
                     /locus_tag="PKO10_04325"
     CDS             complement(<1..523)
                     /gene="hemC"
                     /locus_tag="PKO10_04325"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HNW66407.1"
                     /translation="MEKLVIATRRSQLALWQSEYIKAELLKHYPNMEVELQEFTTKGD
                     KILDVPLAKIGGKGLFTKELEVAMLEGSAHLAVHSLKDVPTQFEDGLVLAAVSKRFNP
                     QDALLSNKYKTIEELPKGAVVGTTSLRRRMAIKMLRPDIELKDLRGNINTRIAKLNAG
                     EYDAIILAATGIQK"
     gene            complement(535..1368)
                     /locus_tag="PKO10_04330"
     CDS             complement(535..1368)
                     /locus_tag="PKO10_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNW66408.1"
                     /translation="MNKLTKLLVLSSIASATLFANDNLVIDFEKKRLSQNPNVKASNI
                     KIFYKKELEAKGWYGYILDFDAVIQDKNMKVKDTLFSDGKVVATDLFDITTSKSLKST
                     IVPNITDKYYQKSKLVAGSEKAKDKIVVFSDPLCPFCAQYVPEVIEFVNKNSDNIALY
                     YYAFPLTHIHPASTTLSKLIDIAKTKLGQEAVLKAYKVDWSKHFAPSTTDEKTILDAF
                     NKELETNIKVQDLSKKEIVANLEKEIASGDDLLVSGTPTIYVNGEIDPTKELYKSLIK
                     K"
     gene            complement(1432..>1883)
                     /locus_tag="PKO10_04335"
     CDS             complement(1432..>1883)
                     /locus_tag="PKO10_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HNW66409.1"
                     /translation="LLIEKVYDRDPILGECFERNFIFDGFSNNILNIISYAKDEDRQL
                     LFKYFGLIKSFAQDIFGLNIELNFKKEEATLLNEPKKENDSFLNETGSMIEDIELGSG
                     CVADMTKSNNEAISSKELQIDDILNSKMLNKAKELFDIKKITVKSRS"
BASE COUNT          576 a          315 c          219 g          773 t
ORIGIN      
        1 gtttttgaat acctgttgct gcaagtatta ttgcatcata ctcacctgca tttagttttg
       61 ctattcttgt attgatattt cctcttaaat ctttaagttc aatatcaggt cttaacatct
      121 ttatagccat tcttcttctt aaacttgtag ttccaacaac agcacctttt ggtaactctt
      181 caattgtttt gtatttgtta cttaaaagtg catcttgtgg attaaatctc tttgaaacag
      241 cagcaagaac aagcccatct tcaaactgtg ttggcacatc ttttagtgag tgaacagcta
      301 aatgagcact tccttcaagc attgcaactt caagctcttt tgtaaaaagc ccttttccac
      361 caatttttgc caaaggaaca tctaaaatct tatctccttt tgtagtaaac tcttgaagct
      421 ctacttccat atttggatag tgttttaaaa gttcagcttt tatatactca ctttgccaaa
      481 gtgctaattg gcttcttcta gttgcaatta caagtttttc cattatttcc tcatttattt
      541 tttaattaaa gatttatata actcttttgt tgggtcaatc tcaccattaa cataaatagt
      601 tggagttcca ctaactaaaa gatcatcacc tgaagctatc tctttttcaa ggttagcaac
      661 tatctctttt ttacttaaat cttgaacttt aatatttgtc tctaactctt tgttaaatgc
      721 atctaaaata gttttttcat cagtagttga tggagcaaaa tgttttgacc aatcaacttt
      781 atatgccttt aaaacagcct cttgacctaa tttcgttttt gctatatcaa ttaatttaga
      841 taatgttgta gatgctggat gtatgtgagt tagagggaaa gcgtagtaat aaagtgcaat
      901 attatcacta tttttattta caaattctat aacttctgga acatattgtg cacaaaatgg
      961 gcaaagagga tctgaaaaaa caacaatttt atctttcgct ttttcacttc cagctactag
     1021 ctttgatttt tgatagtatt tatcagttat atttggaaca atagtagatt taagtgattt
     1081 tgatgttgtt atgtcaaata aatctgttgc tacaactttt ccatcactaa ataaagtatc
     1141 ttttactttc atatttttat cttgaattac tgcatcaaaa tctaaaatat aaccatacca
     1201 tcctttcgct tcaagctctt ttttataaaa aatctttata ttgcttgctt taacatttgg
     1261 gttctgtgat aatctctttt tctcaaaatc aattactaag ttatcatttg caaatagagt
     1321 tgcactagct attgagctta aaactaataa ttttgtaagt ttgttcatat tcttttttcc
     1381 ttttaaataa ttttatagtc tatctttttt tgattaattg aaagttaatt ttcaacttct
     1441 cgattttact gtaatttttt taatatcaaa taactctttt gctttattta gcatttttga
     1501 atttaaaata tcatcaattt gtaactcttt agaagatatc gcttcattat tactttttgt
     1561 catatctgca acacatccac ttcctaactc tatatcttca atcattgaac cagtttcgtt
     1621 taaaaaacta tcattctctt ttttaggctc atttagtaag gtggcctcct cctttttaaa
     1681 atttaactca atatttaatc caaaaatatc ttgagcaaat gatttaataa gtccaaaata
     1741 tttaaaaagg agttgtctat cttcatcttt tgcataagag atgatattta aaatattatt
     1801 tgaaaaacca tcaaaaataa aatttctttc aaaacattct cctaaaattg ggtctctatc
     1861 ataaactttt tctattaata gtt
//
LOCUS       DAOQAN010000230         1871 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_93501_length_1871_cov_3.528084, whole genome shotgun sequence.
ACCESSION   DAOQAN010000230 DAOQAN010000000
VERSION     DAOQAN010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93501_length_1871_cov_3.528084"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(303..1694)
                     /locus_tag="PKO10_04340"
     CDS             complement(303..1694)
                     /locus_tag="PKO10_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014469408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNW66410.1"
                     /translation="MSTIEVTSPFDGKVVGTVKFNTYEEVEAAIDLAHKTFLDRDNWI
                     PKYKRIEILENVMKIMSSQVEELTILCASEGGKPYIDSKVEIQRAINGIKIAIEQIGL
                     QEGHEIAMGHTASSANRIAYTMKEPIGVVAAISAFNHPFNLAVHQVIPAIAVGCPVII
                     RPATQTPMSAIKLVEILKEAGLPNGWAQAVVCDRNAGELLVTSPKTAFFTFIGSGPVG
                     WYLNSKSSPGTRSALEHGGVAPVIVEPDADIEAMIPDLVKGGFYHAGQVCVSVQRIFV
                     HESIANTVASKIAEKASKLVVGNQLDPKTEVGPLINHNEVNRVEEWVNEAVTKGGKIL
                     TGGKRISDSCFEPTVIVNPSDDAIISQKEIFGPVVCVYSYKTLDEAIARANQLDVSFQ
                     AAVFTKNIDTALKSIKRLNATAVMVNDHTAFRVDWMPFGGAKTSGLGLGGIPDSMREM
                     QNQKMMVIKSPVL"
BASE COUNT          615 a          349 c          241 g          666 t
ORIGIN      
        1 accaatttta agcccccaaa ccacaaaaaa taaaccaact acaatgccta ttaaacgtat
       61 agcttctaat gaaactacaa actgtattat acttaatatc gtaacaatta agcctatata
      121 aaatgtaata gttgctttca tagccttttc tttttatttt gtaacattag taaaatatgt
      181 tggttaacgt atttcaaaaa tacacaagtg cttttttaaa aacaagtaat tgactctata
      241 tttaaaaata aaaaaagcta aggctttaaa accttagctt ttaaaaaatc ataaattact
      301 gattataaaa ctggtgattt aataaccatc attttttgat tttgcatctc tctcatagaa
      361 tctggaattc caccaagtcc taatcctgaa gttttagctc caccaaatgg catccagtca
      421 actctaaatg ctgtatggtc atttaccata actgctgttg catttaatct ttttatagat
      481 tttaaagctg tatcaatatt ttttgtaaat actgctgctt ggaatgaaac atctagttga
      541 tttgctctag cgattgcttc atctaaagtt ttatatgaat atacacacac aactggtcca
      601 aaaatctctt tttgagagat aattgcatca tcacttggat taacaataac agttggttca
      661 aaacaagaat cagagattct ttttccacct gttaaaattt ttccaccttt tgtaactgct
      721 tcatttaccc actcttcaac tctgtttact tcattatggt tgattaatgg tccaacttca
      781 gtttttggat caagttgatt tccaacaact agttttgaag ctttttcagc aatttttgat
      841 gcaactgtgt tggcaattga ttcatgaaca aaaattcttt gaactgatac acaaacttga
      901 ccagcatgat aaaatccacc ttttactaaa tctggaatca tagcttcaat atctgcatct
      961 ggctcaacaa taacaggtgc aactccacca tgctctaatg cacttctagt tcctggagat
     1021 gatttagagt ttaaatacca accaactggt cctgaaccaa taaatgtaaa gaatgctgtt
     1081 tttggagatg ttactaataa ctctccagca tttctatcac aaacaacagc ttgagcccaa
     1141 ccatttggta aaccagcctc ttttaaaatc tctactaatt taattgcact cataggagtt
     1201 tgagttgctg gtctaataat tactggacat ccaactgcaa ttgctggaat tacttgatga
     1261 acagcaagat taaatgggtg attaaatgca gaaattgcag caacaacacc tattggctct
     1321 ttcattgtgt atgcaattct atttgcactt gatgctgtgt gacccatagc aatttcatga
     1381 ccctcttgaa gtcctatttg ctcaatagca attttaattc catttatagc tctttgaatt
     1441 tcaactttag agtcaatata tggttttcca ccttcacttg cacaaaggat tgtaagctct
     1501 tcaacttgag aactcataat tttcataaca ttttctaaaa tctctattct tttatatttt
     1561 ggaatccaat tatctctatc taaaaaagtt ttatgtgcta aatcaatagc tgcttcaacc
     1621 tcttcataag tgttaaattt tacagttcca actacttttc catcaaatgg tgatgtaact
     1681 tctatcgtac tcatattttc tcctttgaat tttaatttta aagattaaaa aaggggcttt
     1741 aaatctaaag cccctttcta agattgttta gattaaacta aacaagtttt ttgaggtaaa
     1801 agctcattta agattgcatg atttaaagag taatcaactg ctaaatcaat taaatgaaca
     1861 cctttaccat t
//
LOCUS       DAOQAN010000231         1871 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_93537_length_1871_cov_2.093612, whole genome shotgun sequence.
ACCESSION   DAOQAN010000231 DAOQAN010000000
VERSION     DAOQAN010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93537_length_1871_cov_2.093612"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..830
                     /locus_tag="PKO10_04345"
     CDS             <1..830
                     /locus_tag="PKO10_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HNW66411.1"
                     /translation="YAPFDVDSFIILRQDKTPTYNYACSVDDMLMDISMVIRGEDHVS
                     NTPKQIHIRESLGYTKEIKYVHLPIILNASTGKKMSKRDDASSVKWLIDEGFLPSAIA
                     NYLVLMGNKTPTEIFTLEEAISWFKIENVSRSGAKFDIDKLRFINRKHIELLDDMRLS
                     KILGFADNDIGKLAKLYLEEASTIKEIKSKLNNIFSQKSTLEGFEQESQAIKEALKNA
                     PYFENYDDLKDYVVQKTELKGKNLFKPLRYILTGVENGPNLTDIYPFIKNYIGEITK"
     gene            827..1099
                     /locus_tag="PKO10_04350"
     CDS             827..1099
                     /locus_tag="PKO10_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013135523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HNW66412.1"
                     /translation="MIDALISSFLTIFFSVIFLYKWIVIISALLTWVRPDPYNPIVQM
                     LYRLTEPVYAKMRQYIPTTFGGMDLAPLILIFALIFLETFLQKVLL"
     gene            1104..1844
                     /locus_tag="PKO10_04355"
     CDS             1104..1844
                     /locus_tag="PKO10_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66413.1"
                     /translation="MLKIFKVSLLFSLSLNLFANSITTDFMQKDFKITYEWLAEKPKS
                     SAKDFFILQYLENEDLSYENAKKVYDMRNGRNALLDKAFKQKFNEKISPEDRFCYNAS
                     ILELKNSDSRCIALGLASLKKASDLSKEDLAFFISKLDTYPTLKNNLILISSNDIYKK
                     LINGSTDKFLEIFFEVSDAYRYKYLNQSINPTFLHKIAMHKDFEKFLRTVVYDKKLNN
                     IQKSLDNLKTERMLTTNLQFLLGINAVR"
BASE COUNT          733 a          222 c          268 g          648 t
ORIGIN      
        1 aatatgctcc ttttgatgtt gatagtttta ttattttaag acaagataaa actcctacat
       61 ataactatgc ttgtagtgtt gatgatatgc taatggatat atctatggta attagaggag
      121 aagatcatgt ttcaaatact ccaaaacaga tacatattag agagagttta ggttatacaa
      181 aagagataaa atatgttcat ttaccaatta ttttaaatgc aagtacaggt aaaaaaatga
      241 gtaaaagaga tgatgcaagt agtgtaaagt ggctaattga tgaaggattc ctaccaagtg
      301 ctattgcaaa ctatcttgta cttatgggaa ataaaactcc aacagagatt tttactctag
      361 aagaagcaat ttcatggttt aaaatagaaa atgtctcaag aagtggcgca aagtttgata
      421 ttgataagct aagattcata aatagaaaac atatagaact attagacgat atgagactat
      481 caaaaatttt aggttttgca gataatgata ttggaaaatt agcaaaacta tatttagaag
      541 aagcaagtac aataaaagag ataaaatcaa aactaaataa tattttttct caaaaatcta
      601 ctcttgaagg ttttgaacaa gagagtcaag ctatcaaaga agcgttaaaa aatgcaccat
      661 attttgagaa ttatgatgat ttaaaagatt atgttgttca aaaaacagaa cttaaaggta
      721 agaatctatt taaaccatta agatatattc taacaggtgt tgaaaatgga ccaaatctaa
      781 cagatattta tccatttatt aaaaactata ttggggagat tacaaaatga tagatgcact
      841 tattagctca tttttaacta ttttttttag tgttattttt ctttacaagt ggattgttat
      901 tatttcagca cttttaactt gggttagacc agatccatac aatccaatag ttcaaatgct
      961 ttatagatta acagagcctg tttatgcaaa aatgagacaa tatattccaa caacttttgg
     1021 aggaatggat ttggctccac ttattttgat ttttgctctt atatttttag agacattttt
     1081 acaaaaagtt cttctataga tttatgttaa aaatatttaa agtatctctt ttattttcgc
     1141 ttagtctaaa tctttttgca aatagtataa ctacagattt tatgcaaaaa gatttcaaaa
     1201 taacttatga atggcttgca gaaaaaccaa aatcaagtgc taaagatttt tttatattac
     1261 aatatcttga aaatgaagat ttaagttatg aaaatgcaaa aaaagtttat gatatgagaa
     1321 atggaagaaa tgctcttctt gataaagcct ttaaacaaaa attcaatgaa aaaatatctc
     1381 cagaagatag gttttgctac aatgcttcta ttttagaact taaaaatagc gattcaagat
     1441 gtattgctct tggtttagct tcacttaaaa aagcatctga cttatcaaaa gaggatcttg
     1501 ctttttttat atcaaagctt gatacttacc ctactttaaa aaataatctt atacttatat
     1561 catcaaatga tatttataaa aaacttataa atggctctac tgataaattt ttagaaattt
     1621 tttttgaagt tagtgatgca tatagatata aatatttaaa tcaatctata aatccaactt
     1681 ttttacataa aattgctatg cataaagatt ttgaaaaatt tttacgaact gttgtttatg
     1741 ataaaaaatt aaataatata caaaaatctc ttgataacct aaaaactgaa agaatgttaa
     1801 caacaaatct tcaattttta ttaggtataa atgctgtaag gtagtatttg ctgcctgcat
     1861 agaaccggaa t
//
LOCUS       DAOQAN010000232         1866 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_93887_length_1866_cov_2.685809, whole genome shotgun sequence.
ACCESSION   DAOQAN010000232 DAOQAN010000000
VERSION     DAOQAN010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93887_length_1866_cov_2.685809"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="PKO10_04360"
     CDS             complement(<1..444)
                     /locus_tag="PKO10_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HNW66414.1"
                     /translation="MIKESIFKPISCGETLPVNNIHAVSTSMPTLQDVIDYEEQTPQI
                     LEKISVAYPRFIVHPYLKKLAIYLKSKYKVSDNYELILLSSKKAVKVVSSRFYINNPI
                     DIDEDFGVIMVLKGRQYQKVLKFIQHVGYNLSSRLAEDYLYNLGKI"
     gene            complement(441..1592)
                     /locus_tag="PKO10_04365"
     CDS             complement(441..1592)
                     /locus_tag="PKO10_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HNW66415.1"
                     /translation="MNKSLETMLCHLKDFAPFKEPTNSSHFPIYNTATFDLKNQNGDK
                     IYDYTRSDNPTRATLENFFAFAENGYGAVCTHTGIASVSLLFETVLKANSSILVEADC
                     YGGTFRLLKVFKEKYNINVNFTDFTDLNMVEHILKTNNIDLVLCESPTNPGLKIIDLK
                     EISKLCKVHNSLFAVDNSLATFISQKPLDLGADFSLFSTTKFISGHGSVIAGAIVAKT
                     KENSEKLHYYSNALGRNQNPLDVHLISLGISSLKVRMKASEKLAKKFAKWLEKQDFIE
                     KVTFPALKSHPQRALAKKQMKIIPSVFCADFKSVELAEKFIENAKIFGEKCSFGSADS
                     RVEIPSKISHASFSKEELKAIGISDSTVRFSIGFEDLKDLKEDLLEAIK"
BASE COUNT          647 a          262 c          254 g          703 t
ORIGIN      
        1 aatttttcca agattatata gataatcttc agctaatctt gaagataaat tgtacccaac
       61 atgttgaata aattttaaaa ctttttggta ctgtcttcct tttaaaacca taattacacc
      121 aaaatcttca tcaatatcta ttggattatt tatataaaat ctagagctta caacttttac
      181 tgcctttttg ctgctaagaa gaataagttc ataattatca ctaactttat attttgattt
      241 taaataaatt gctagttttt taagataagg atgaactata aatctaggat atgcaacact
      301 tattttttct aaaatctgcg gtgtttgttc ttcataatca attacatctt gaagtgttgg
      361 catagatgta gaaactgcat gaatgttgtt tactggtaaa gtctcaccac aagagattgg
      421 tttaaaaata gactctttta tcattttatg gcttctaata aatcctcttt taaatctttt
      481 aaatcttcaa atccaataga aaatcgtacc gtactatcag atattccaat tgcttttaac
      541 tcctcttttg aaaaagatgc atgagatatt ttagaaggta tttcaactct actatcagca
      601 cttccaaaag agcatttttc tccaaatatt tttgcatttt ctataaattt ttctgctagt
      661 tctacacttt taaaatcagc acaaaaaaca ctaggaatta ttttcatctg tttttttgct
      721 aaagctcttt gtggatgtga tttaagtgct ggaaatgtaa ctttttctat aaaatcttgt
      781 ttttcaagcc atttagcaaa tttttttgct aatttttcgc ttgctttcat tctaactttt
      841 aggctactaa ttccaagtga tattagatga acatcaagag gattttggtt tcttcctaag
      901 gcatttgagt agtagtgaag tttttctgag ttctctttag ttttggcaac aatagcacct
      961 gcaattacgc ttccgtgacc acttataaat ttagttgtag aaaagagtga aaaatcagct
     1021 cctaaatcaa gaggtttttg actaataaaa gttgcaagtg agttatcaac tgcaaaaaga
     1081 gaattatgca ctttacaaag tttagatatc tcttttaaat ctattatttt taagcctgga
     1141 tttgtaggac tttcacaaag aactaaatct atattattag tttttaaaat atgttcaacc
     1201 atatttaaat ctgtaaaatc tgtaaagttt acattaatat tgtatttttc tttaaaaact
     1261 tttaaaagcc taaatgttcc accataacaa tcagcttcaa ctaaaataga tgaatttgct
     1321 tttaaaacag tttcaaataa taaagataca gatgctattc ctgtgtgagt gcaaacagct
     1381 ccatatccat tttcagcaaa agcaaaaaag ttttcaagtg tagctcttgt aggattatcg
     1441 cttcttgtat aatcatatat tttatcgcca ttttggtttt ttaaatcaaa agttgctgtg
     1501 ttgtatattg gaaagtgtga agagtttgta ggctctttaa aaggtgcaaa atcttttaaa
     1561 tgacaaagca tagtttctaa acttttattc ataaaaatcc ttaaattggc taatttttgg
     1621 gaaattgtac ccaaataaat ttaatcttat ttgatacaaa aatttgctat aaatttatat
     1681 gaaattttat ttatataaaa ggataagaga tgcttaaaag tatattatta ttaatattta
     1741 taatattttt tcaaggatgt gcaacttgga gtggagtaaa acaagatagt aaaacagctt
     1801 ggaaagctac aaaagagaca acaactgaag ttgtaaaaga tgtaaaaaaa tcaattcacg
     1861 aagcaa
//
LOCUS       DAOQAN010000233         1866 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_93896_length_1866_cov_2.500828, whole genome shotgun sequence.
ACCESSION   DAOQAN010000233 DAOQAN010000000
VERSION     DAOQAN010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93896_length_1866_cov_2.500828"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..914
                     /locus_tag="PKO10_04370"
     CDS             <1..914
                     /locus_tag="PKO10_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HNW66416.1"
                     /translation="LSLLYKNSPDNLRLVMIDPKMLEFSMYNDIPHLLTPVITKAVDA
                     INALANMVGEMERRYSLMAKTKTKNIENFNEKAQKEGLPSMPYIVVVIDELADLMMTS
                     GKDVEYSIARLAQMARASGIHLIVATQRPSVDVVTGLIKANLPSRLSYKVGQKIDSKI
                     ILDSMGAESLLGRGDMLFTPPGTPGIVRIHAPWSTETEIEKVVEFLKDQREVEYDMNF
                     IKDKNLGSSSNGSKGAKNISDEDIVLDDLYEDAKEVIITDKKTSISYLQRRLKIGYNR
                     AATIIEQLEKTGVLSEVDARGNREILV"
     gene            938..1645
                     /locus_tag="PKO10_04375"
     CDS             938..1645
                     /locus_tag="PKO10_04375"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNW66417.1"
                     /translation="MCFENKVVFITGANGGLAKSFIEALLKENAKKIYCTARDLEKLA
                     ELKKLSSKIEIFNLDITKKDELDEIASKIDNIDYLINNAGVNSLKRVFDDSKIDFEVN
                     LFGTLNSCQILSKKLNKNGSIVNITSILALINLPIMGLYCASKSALHSLTQAFRAELQ
                     KENIKVYEVLPGPIDTNMTKGQDMPKSSTSYIVSNIIDGIKNEEFEIYPDDFSKMVKR
                     RLKDDKGNLEKEFLNSL"
     gene            complement(1677..1829)
                     /locus_tag="PKO10_04380"
     CDS             complement(1677..1829)
                     /locus_tag="PKO10_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66418.1"
                     /translation="MEILLLISYFIFFLIALILTFTSFKYFITMWRFKNRKKDGSEDD
                     NFKKFD"
BASE COUNT          760 a          201 c          302 g          603 t
ORIGIN      
        1 tattatcact gctttataaa aactcaccag ataatttgcg acttgtaatg atagacccaa
       61 aaatgcttga gttttcaatg tacaacgata ttccacatct tttaactcca gttattacaa
      121 aagctgttga tgcaataaat gcattagcaa atatggttgg ggagatggaa agaaggtact
      181 ctttaatggc aaaaactaaa acaaaaaata ttgaaaattt caatgaaaaa gcacaaaaag
      241 aaggacttcc ttctatgcct tatattgttg ttgtaattga tgagttagct gatttaatga
      301 tgacaagcgg aaaagatgtt gaatactcaa ttgcaagact tgctcaaatg gcaagagcta
      361 gtggaattca cttaatagta gcaactcaaa gaccatcagt tgatgttgta actggactta
      421 taaaagcaaa tttaccaagc agattatctt ataaagtggg tcaaaaaata gattcgaaaa
      481 ttatacttga ttctatgggt gctgagtcgc ttttaggaag gggagatatg ttgtttacac
      541 ctccaggaac tcctggaatt gtgagaattc atgctccttg gagtactgaa actgaaatag
      601 aaaaagttgt tgaattttta aaagatcaaa gagaagttga atatgatatg aattttatta
      661 aagataaaaa tttaggttca agttctaatg gttcaaaagg tgctaagaat attagtgatg
      721 aagatatagt tttagatgat ttgtatgaag atgcaaaaga ggtaataata acagataaaa
      781 aaacatctat ctcatatctt caaagaagat taaaaatagg gtacaatagg gctgcaacaa
      841 ttattgaaca actagaaaaa acaggtgttc taagtgaagt tgatgcaaga ggaaatagag
      901 aaattttagt ttaataaatt taaataagga tataaatatg tgttttgaaa ataaagtagt
      961 atttattaca ggtgcaaatg gtggtttggc taaatctttt attgaggctt tattaaaaga
     1021 gaatgcaaaa aaaatatatt gtacagcaag agatttagaa aaattagctg aattaaaaaa
     1081 attatcatca aaaatagaga tttttaattt agatattaca aaaaaagatg aattggatga
     1141 gatagcttct aaaatagata atattgatta tttaataaat aatgctggtg taaatagctt
     1201 aaaaagagtt tttgatgata gtaaaattga ttttgaagta aatctatttg gaacactaaa
     1261 tagttgtcaa atattatcaa aaaaattaaa taaaaatggc tctattgtta atattacatc
     1321 aattttagct ttaataaatc ttcctataat gggactttac tgtgcttcaa aaagtgcttt
     1381 acactcttta actcaagctt ttagagcaga gctacaaaaa gaaaatataa aagtgtatga
     1441 agttttacca ggaccaattg atacaaatat gacaaaaggg caagatatgc caaaaagttc
     1501 aacttcttat atagtttcaa atattataga tggaataaaa aatgaagagt ttgaaatcta
     1561 tcctgatgat ttttctaaaa tggttaaaag aagactaaaa gatgataaag gaaatttaga
     1621 aaaagagttt ttaaactctt tgtaaaacaa ttaaataagg ttatttataa tcttatttaa
     1681 tcaaactttt taaagttgtc atcttcactt ccatcttttt ttctattttt aaatctccac
     1741 attgtaataa aatatttaaa acttgtaaaa gtaagaatca aagcaattaa aaaaaatata
     1801 aagtatgaaa tcaacaataa tatttccata agtagacctt ttattggttt tgactaaatt
     1861 ttatca
//
LOCUS       DAOQAN010000234         1861 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_94257_length_1861_cov_3.104651, whole genome shotgun sequence.
ACCESSION   DAOQAN010000234 DAOQAN010000000
VERSION     DAOQAN010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94257_length_1861_cov_3.104651"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1370)
                     /locus_tag="PKO10_04385"
     CDS             complement(<1..1370)
                     /locus_tag="PKO10_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HNW66419.1"
                     /translation="MQLENYGNFPQTIPLIIEDEIFLYPFMITPLFLENQENIKAVEN
                     AIEFNRLVMIAVSKAGKEGVREKDSFYDVGVVGNVMRKISLPDGKVKVLFQGVSKAKI
                     INFEPTSSIFATVDILAEETQNDIELKSLINILIDNIKKLSRLNNKFPADLIKAIEEN
                     DVPSRVADLVSSVLKIKKDEAYKIFSNTNLEQRIIGIIEIVKNEIESYKIQKEITQKV
                     NSKIEKSHKDYFLKEQIKAIQKELGADNQREEDIKSFKKRLKAKKCFMGKEAYKETKK
                     QIEKLSRMNTDSPDASLLQTYVEMVLDIPFGEYSDSKISVSSVEKQLNKDHYSLEKAK
                     ERIAEYFAVKQLLEQRNIEDLKSKGTVLCFVGPPGVGKTSLANSIANALARPLVRVAL
                     GGMEDVNELRGHRRTYVGAMPGRLIKGLIDAKKMNPVIVLDEIDKLGSNHRGDPSAVM
                     LEILDP"
     gene            complement(1410..>1861)
                     /gene="bamD"
                     /locus_tag="PKO10_04390"
     CDS             complement(1410..>1861)
                     /gene="bamD"
                     /locus_tag="PKO10_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014473435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HNW66420.1"
                     /translation="PYIPTAMLILVNAHMSDEQYALANFYLDEYIKRFSLSKDIDYAR
                     YMKIKANFMGFKQQFRDQQLIDDTLANIIDFKYKYPNSPYMPLVDTMNARLFMAKASM
                     DKEIAALYERRDKPKAAELYMDKAKNSWVDPNEIKPVEVPFYRAIFE"
BASE COUNT          557 a          336 c          208 g          760 t
ORIGIN      
        1 tcaggatcta agatctctaa cataacagca gaagggtcac ctctgtggtt acttcctaat
       61 ttatcaatct catcaagaac aataactgga ttcatttttt ttgcatcaat taaaccttta
      121 ataagtcttc ctggcattgc tccaacataa gttcgtctat gtcctcttaa ttcatttaca
      181 tcttccattc cacccaaagc aactcttaca agtggtctag caagagcatt tgctattgaa
      241 tttgctagtg aagttttacc aactcctgga ggtcctacaa aacaaagaac cgtacctttt
      301 gattttaaat cttcaatatt tctttgctct aaaagctgct ttacagcaaa atattcagcg
      361 attctctctt ttgctttttc taaagagtag tgatctttat ttaattgctt ttcaacacta
      421 gaaactgata ttttactatc tgaatattca ccaaaaggaa tatctaaaac catctctaca
      481 taagtctgaa gaagtgaagc atctggagaa tctgtattca ttcggcttag tttttctatt
      541 tgcttttttg tctctttata ggcttcttta cccataaaac actttttcgc ttttaatctc
      601 tttttgaaag attttatatc ttcctctctt tgattatcag ctcctaactc tttttgaata
      661 gcttttattt gctcttttaa gaagtaatct ttatggcttt tttctatttt tgaatttact
      721 ttttgagtta tctctttttg tattttatat gactctatct cattttttac aatctcaata
      781 atacctataa ttctttgttc taaatttgta tttgaaaaaa tcttatatgc ttcatctttt
      841 tttattttta aaacagagga taccaaatca gcaactctgc ttggtacatc attctcttct
      901 atagctttta ttaaatcagc tggaaatttg ttatttaatc ttgatagttt ttttatatta
      961 tcaatcaaaa tatttattaa tgatttcaac tcaatatcat tttgtgtctc ttcagcaaga
     1021 atatctactg tagcaaatat actagaagtt ggttcaaaat ttataatttt tgcctttgaa
     1081 acaccttgaa aaagaacttt taccttacca tctggtaaag atatttttct cataacattt
     1141 ccaactacac caacatcata aaaactatcc ttttctctaa caccctcttt tcccgctttt
     1201 gaaactgcta tcattacaag tctattaaac tcaattgcat tttcaactgc tttaatattc
     1261 tcttgattct ctaaaaacaa aggcgtaatc ataaatggat ataaaaaaat ctcatcttct
     1321 ataatcaatg gtattgtttg tggaaaatta ccataatttt ctaattgcat atcttactct
     1381 aactccctta ttttatacat tttatatatt tattcaaaaa ttgctctgta gaatggtact
     1441 tctactggtt ttatttcgtt tggatctacc caagaatttt ttgccttatc catataaagt
     1501 tctgctgctt ttggtttatc tcttctttca tataatgctg ctatctcttt atccatagaa
     1561 gcttttgcca taaaaagtct tgcattcata gtatcaacaa gtggcatata tggtgaattt
     1621 ggatatttgt atttaaagtc tataatattt gccaaagtat catctattag ctgttgatct
     1681 ctaaattgtt gcttaaaccc cataaaattt gctttaattt tcatatatct tgcataatca
     1741 atatctttac ttaagctaaa tcttttaata tattcatcta aataaaaatt tgccaaagca
     1801 tactgctcat ctgacatatg tgcatttaca agtataagca tagctgttgg aatatatggt
     1861 g
//
LOCUS       DAOQAN010000235         1859 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_94441_length_1859_cov_2.177938, whole genome shotgun sequence.
ACCESSION   DAOQAN010000235 DAOQAN010000000
VERSION     DAOQAN010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94441_length_1859_cov_2.177938"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..671
                     /locus_tag="PKO10_04395"
     CDS             <1..671
                     /locus_tag="PKO10_04395"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HNW66421.1"
                     /translation="YFLFWLVFGVVLMVAVREAKMLYGLEDKSIYIYVLLLWVAVYFY
                     PMPVDLIFIVAIGYASQLAYKRKLDKKMILPLLYPTASFVFLISLYSEFGVMVLFWLL
                     IIVAATDTGAYFAGKTFGRTPFCETSPNKTLEGVVGGMIFAVIMGIFTSINEVGILGA
                     IIVSAIVSLSSVFGDLYESYLKREAGVKDSGNILPGHGGVLDRVDGYLFGAIVMLVLL
                     RIII"
     gene            668..1735
                     /gene="dxr"
                     /locus_tag="PKO10_04400"
     CDS             668..1735
                     /gene="dxr"
                     /locus_tag="PKO10_04400"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HNW66422.1"
                     /translation="MILLGSTGSIGVNTLEIAKKFALNVEVLVAGRNIDLLNSQIKEH
                     NPKKVIIASKYDIKKVNHKNVFFGEEAILEAIEDSSSKTVVNALVGFLGLKPTLKAIE
                     CGKKIALANKESLVVAGKFIDQKNLSAIDSEHFGLWYLLQDKKIDSMTITASGGSFRD
                     YPLESLKNVSIKEALNHPNWKMGNKITIDSATMTNKMFELIEAAWLFGTKKLDAVIEP
                     KSLVHALINFKDGSTTAHIANASMQLPISYAILGRVDCEILKPIDLLEISSLEFKKIE
                     ESRYPIWSLKDEILNNLDLGVVLNAANEVAVSKFLSGKIGFLDISKISLEAINRFNSL
                     KASNIEDIFEIDKEVRRYFDS"
BASE COUNT          663 a          195 c          343 g          658 t
ORIGIN      
        1 catactttct attttggtta gtgtttggag tggttcttat ggttgcagta cgtgaagcaa
       61 aaatgttata tggattagaa gataagagca tatatattta tgttttatta ctttgggtag
      121 ctgtatattt ctatcctatg cctgttgatt taatattcat agtagcaatt ggttatgctt
      181 cacaattagc atataaaaga aaacttgata aaaagatgat tttacctctt ctttatccaa
      241 ctgcatcatt tgttttttta atctctttat atagtgaatt tggagtaatg gtactatttt
      301 ggctactaat aatagttgca gcaactgata caggagctta tttcgctgga aaaacttttg
      361 gaagaactcc attttgtgaa acaagcccaa ataaaacttt agagggtgtt gtaggtggta
      421 tgatttttgc tgtaattatg gggattttta cttctataaa tgaagttggg attttaggag
      481 ctattatagt ttcagctatt gtatcactat cttcagtttt tggagattta tatgaaagtt
      541 atctaaaaag agaagctgga gtaaaagata gtggaaatat tttaccagga catggtggag
      601 ttttagatag agttgatggg tatttatttg gagcgatagt tatgttagtt ttattaagga
      661 taattatttg atacttcttg gaagtacagg ttctattgga gtaaatactc tagaaattgc
      721 gaaaaaattt gctctaaatg ttgaggtttt agtagctgga agaaatattg atttactaaa
      781 ttctcaaata aaagagcata atccaaaaaa agtgataatt gcttctaaat atgatataaa
      841 aaaagtaaat cacaaaaatg tattttttgg ggaagaagct atactagaag caatagaaga
      901 tagctcttca aaaactgttg taaatgctct tgttggtttt ttaggattaa aaccaacatt
      961 aaaagcaata gagtgtggta aaaaaattgc tttagcaaat aaagagtcat tagttgttgc
     1021 tggaaaattt atagaccaaa aaaatttaag tgctattgat agtgaacatt ttggtctttg
     1081 gtatttactt caagataaaa aaatagattc tatgacaatc acagcaagtg gcggttcttt
     1141 tagagattat ccacttgaat ctttgaaaaa tgtatctata aaagaggctc ttaatcatcc
     1201 aaattggaaa atgggaaata aaataacaat agatagcgca actatgacaa ataaaatgtt
     1261 tgagttaata gaggctgctt ggctctttgg tacaaaaaaa ctagatgctg ttattgagcc
     1321 aaaatctttg gttcatgcat taataaattt caaagatgga agtacaacag ctcatatagc
     1381 aaatgcatct atgcaacttc caatatctta tgcaatttta ggaagagtcg attgtgagat
     1441 tctaaaacct attgatttac ttgaaatatc atctttagag tttaaaaaaa tagaagagag
     1501 tagatatcca atttggtctt taaaagatga gattctaaac aatcttgatt taggtgttgt
     1561 tttaaatgca gcaaatgaag ttgctgtttc aaaattttta agtggaaaaa taggtttctt
     1621 ggatatttca aaaataagct tagaagctat aaatagattt aatagtttaa aagcttcaaa
     1681 tattgaagat atttttgaaa tagataaaga ggtaaggaga tattttgact cttgatcttt
     1741 taattccttt tggaatttta tttttcttag tggtgtatct aatatattca agagcaaagt
     1801 ttgagaaaaa tattgtaaag ctatatgaag ataagttaga agattggaaa aaatactca
//
LOCUS       DAOQAN010000236         1857 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_94591_length_1857_cov_2.228635, whole genome shotgun sequence.
ACCESSION   DAOQAN010000236 DAOQAN010000000
VERSION     DAOQAN010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94591_length_1857_cov_2.228635"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..962
                     /locus_tag="PKO10_04405"
     CDS             <1..962
                     /locus_tag="PKO10_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNW66423.1"
                     /translation="NRQVTNKDKLAQIIQQLVKGTENIKELEFLVKEARNIGLSGINT
                     YFNPILKLYNYTNSLGLASLKEIDEELLSDFLASQTSSLSDASKKNHRIALLSFFSYI
                     DKQNENEDGSSYLFKIELKNWGGLSGKSGSKLPSFMNKDEIDRFLSAINNFEFSDNTA
                     YRNRLIIKIIIYTGIRVSEMLNLKLKDIFNEKDVYMLQIRGKGNKPRVVMIKKDIIEH
                     ELQNWLTQRVCNSDILVCNQKGERLTQAYVSRIVENILASAGIRKEKNGAHMLRHSFA
                     TLLYSKHHDLILVQEALGHADINTSRIYTHFDKERLRKTTDIF"
     gene            1038..>1857
                     /locus_tag="PKO10_04410"
     CDS             1038..>1857
                     /locus_tag="PKO10_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HNW66424.1"
                     /translation="MIEIDYYELLEIEKTSDKAAIKKAYRKLAMQYHPDKNPNDKEAE
                     EKFKSINEAYQVLSDDEKRALYDRYGKAGLEGHGGRSSGFGGFDDLGSIFEEMFGFSR
                     NHSKKERKTYNYNLDTAVEVKLEFNEAVFGCKKEINYKYKTACKPCNGTGAKDAKIEN
                     CRTCGGVGQVHSRQGFMTFAQTCPTCSGSGQSKANSCKSCSGTGFEEIKGNFTVDIPE
                     GVNDGMRIRVSNKGNIAPNGQRGDLYIQTKVKEDGHFVRHDDDIYFEAPIFFTQV"
BASE COUNT          744 a          198 c          327 g          588 t
ORIGIN      
        1 caaatagaca agttacaaac aaagataaat tagcacaaat aatccagcaa ttagtaaaag
       61 gaacagaaaa tataaaagag cttgaatttt tggtaaaaga agcaagaaat attggattat
      121 ctggaataaa tacatatttt aatcctattt taaaactata taactataca aatagtttag
      181 gattagcttc acttaaagag attgatgaag agttattaag tgatttttta gctagccaaa
      241 ctagttctct atcggatgct tctaaaaaga accatcgtat cgccctacta tctttcttct
      301 catatataga taaacaaaat gaaaatgaag atggaagctc atatttattt aaaatagagt
      361 taaaaaattg gggtggatta agtggaaaaa gtggttcaaa actcccctct tttatgaata
      421 aagatgaaat tgatagattt ttatctgcta ttaataattt tgaattttca gataatacag
      481 cttatagaaa tagacttatt ataaaaataa taatatatac aggaattaga gttagtgaga
      541 tgttaaatct caaattaaaa gatattttta atgaaaaaga tgtttatatg cttcaaattc
      601 gtggaaaagg aaataaacca agagttgtaa tgattaaaaa agatattatt gaacatgaat
      661 tacaaaattg gctaactcaa agagtttgta atagtgatat tttagtttgt aatcaaaaag
      721 gtgaaagatt aactcaagct tatgttagtc gtattgttga aaatatctta gcaagtgctg
      781 gtattagaaa agagaaaaat ggagcacata tgttacgtca ctcatttgca actttacttt
      841 attcaaaaca tcatgattta atacttgttc aagaggcttt aggtcatgca gatataaaca
      901 caagcagaat ttatacgcac tttgataaag aaagattaag aaagactaca gatatttttt
      961 aacttaatat tgttttatca ataattggat aaaataataa ctttattata aaattaatac
     1021 atttttggag attaaaattg attgaaattg attattatga acttttagag attgaaaaaa
     1081 catcagataa agcagctata aaaaaagctt atcgaaaact tgctatgcag tatcatcctg
     1141 ataaaaatcc aaatgataaa gaagctgaag agaaattcaa atctataaat gaggcttatc
     1201 aagttttaag tgatgatgaa aaaagagctt tatatgatag atatggaaaa gctggtttag
     1261 agggtcatgg tggacgaagt tctggttttg gtggttttga tgatttaggt tctatttttg
     1321 aagagatgtt tggtttttca agaaatcata gcaaaaaaga gagaaaaact tataactata
     1381 atcttgatac tgccgttgaa gttaaattag agtttaacga agcagttttt ggttgcaaaa
     1441 aagagattaa ctataaatat aaaacagcct gtaaaccttg taatggaact ggtgcaaaag
     1501 atgctaagat tgaaaactgt agaacttgtg gtggagttgg acaagttcat tcaagacaag
     1561 gttttatgac atttgcacaa acatgtccaa cttgtagtgg tagtggacaa tcaaaagcaa
     1621 atagttgtaa atcatgttct ggtactggtt ttgaagagat taaaggaaat ttcactgttg
     1681 atattccaga aggtgtaaat gatggaatga gaataagagt ttcaaataag ggaaatatag
     1741 caccaaatgg acaaagagga gatttatata tccaaacaaa agtaaaagaa gatggtcact
     1801 ttgtaagaca tgatgatgat atatattttg aagcccctat tttcttcact caagtag
//
LOCUS       DAOQAN010000237         1856 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_94636_length_1856_cov_3.392004, whole genome shotgun sequence.
ACCESSION   DAOQAN010000237 DAOQAN010000000
VERSION     DAOQAN010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94636_length_1856_cov_3.392004"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="PKO10_04415"
     CDS             complement(<1..540)
                     /locus_tag="PKO10_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014467925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HNW66425.1"
                     /translation="MNNGKIAVIGAGKWGQALHFALNSKQECFITSRTKRDIKNFVPL
                     DFALSCEYLIIAIPAQEIKTWLKENFVFKGQKILVASKGIEANSGEFLNEIYSSFVPD
                     ENIGFISGPSFAAEVIKGLPCALVINSKSKKIYEEFSKFFPNFIKTYYSSDVIGAEIA
                     GAYKNVLAIASGICEGLNLG"
     gene            complement(540..>1856)
                     /gene="gatB"
                     /locus_tag="PKO10_04420"
     CDS             complement(540..>1856)
                     /gene="gatB"
                     /locus_tag="PKO10_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013459685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HNW66426.1"
                     /translation="TCLALPGGLPVLNKEAVHKAIMLGTALKSQINQVSVFDRKNYFY
                     PDLPTGYQISQLSTPVVGLGELTIDFPDGSQKTIGVTRAHLENDAGKNIHSGNHSQVD
                     LNRAGTPLLEIVSEPDMRSAEEAILYLKKLHSIVRYLGISDANMQEGSFRCDVNVSIR
                     PKGDTNFYTRCEIKNMNSFRFIEKAIAYEVNRHIEAWEDGVHSTEIVQETRLFDTTTG
                     ETRSMRGKEDAADYRYFPDPDLLPLIITDEMLKEYSKIPELPDEKKARFVKEYGLKEY
                     DASVITSSLEMANYFDEMMSEGISAKNATTWLTVELQGRLKEGVTIEESPIDAKTLAT
                     IVKRIEDNTISGKAAKEVLDDLIANSSKDVDATILKLGLKQVSDDGALFAIIDEILAA
                     NADKVAEYKAGKEKMFAFFVGQTMKASKGSANPQKVNELIKERLSK"
BASE COUNT          569 a          355 c          236 g          696 t
ORIGIN      
        1 tccgagattt aatccttcac aaattccact tgcaattgct aaaacatttt tataagctcc
       61 tgctatctca gctccaataa catctgaact ataatatgtt tttataaaat ttgggaaaaa
      121 tttactaaac tcttcatata ttttttttga ttttgaatta atcaccaaag cacaaggcaa
      181 accttttata acctcagcag caaaagatgg tccagagata aatccaatat tttcatctgg
      241 tacaaatgat gaataaatct cgtttaaaaa ctctccacta tttgcttcaa tcccttttga
      301 agcaacaaga attttttgac ctttaaaaac aaaattctct tttagccaag tttttatctc
      361 ttgagcggga attgcaataa tcaaatattc acaagatagg gcaaaatcta aaggtacaaa
      421 attttttata tctctttttg ttcttgaagt tataaaacac tcttgttttg aatttagtgc
      481 aaaatgaaga gcttgtcccc attttccagc accaattact gcgattttgc cattattcat
      541 tatttgctta atctctcttt tattagctca tttacttttt gaggatttgc acttccttta
      601 cttgctttca tagtttgtcc tacaaaaaat gcaaacatct tctcttttcc agctttatat
      661 tctgctactt tatcagcatt tgcagctaaa atttcatcaa taattgcaaa taaagctcca
      721 tcatctgaaa cttgtttaag tcctaattta agaattgtag catctacatc ttttgaagag
      781 tttgcaatta aatcatctaa aacctctttt gcagcttttc cagagattgt attatcttca
      841 attcttttta caattgtagc tagtgttttt gcatctattg gtgactcttc aatagtaaca
      901 ccctctttta atcttccttg tagctcaaca gttagccaag ttgtagcgtt tttagcacta
      961 attccttcac tcatcatctc atcaaaatag tttgccatct ctaaagatga agttataaca
     1021 gacgcatcat actctttaag tccatactct tttacaaatc tagctttttt ctcatctgga
     1081 agttctggaa tttttgaata ctcttttagc atttcatctg taataattaa aggtaaaagg
     1141 tctggatctg gaaaatatct ataatcagcg gcatcttctt tacctctcat acttcttgtc
     1201 tctccagtag ttgtatcaaa aagtctagtc tcttgtacaa tttcagttga gtgaacacca
     1261 tcttcccaag cttcaatatg tctatttacc tcatatgcaa ttgctttttc aataaatcta
     1321 aatgagttca tatttttaat ttcacatcta gtgtaaaagt ttgtatcacc ttttggtctt
     1381 atagatacgt taacatcgca tctaaaactt ccctcttgca tatttgcatc actaattcct
     1441 aaatatctta caattgagtg aagtttttta agatataaaa tagcctcttc tgcacttctc
     1501 atatcaggtt ctgaaacaat ttcaagaagt ggagttccag cacgatttaa atcaacttgt
     1561 gaatggttac cactatggat attttttcca gcatcatttt ctaaatgagc tcttgtaact
     1621 ccaatagttt tttgacttcc atctggaaaa tcaatagtta gttcaccaag accaacaaca
     1681 ggagtgctta attgagatat ttgataacca gttggtaaat ctggatagaa atagtttttt
     1741 ctatcaaaaa ctgatacttg atttatttgt gatttcaatg cagtcccaag cataatagct
     1801 ttatgaactg cctctttatt taaaacagga agtcctcctg gaagtgctag acaagt
//
LOCUS       DAOQAN010000238         1850 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_95174_length_1850_cov_2.198329, whole genome shotgun sequence.
ACCESSION   DAOQAN010000238 DAOQAN010000000
VERSION     DAOQAN010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95174_length_1850_cov_2.198329"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="PKO10_04425"
     CDS             <1..289
                     /locus_tag="PKO10_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66427.1"
                     /translation="SLSVFAIILSFRFPLFICETTSFTFLINASSCFNRLFVLSIFSF
                     TTFVIIPISSSDFISILETSELSLFRYLKNDKSPFSNLSKGEIISFVIKMR"
     gene            complement(261..1370)
                     /locus_tag="PKO10_04430"
     CDS             complement(261..1370)
                     /locus_tag="PKO10_04430"
                     /inference="COORDINATES: protein motif:HMM:NF019874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HNW66428.1"
                     /translation="MKNLSIRVKLLSIVIITIILVSTIIATKSIYEVNNLTNQTIEEY
                     RQNAFNASIEELKNYTAFAQNIAKSAYEEAKIENIKARKGAYLKSQTDFLFAMISKIY
                     DEQKNKMPEAELKKILLEAIGSVRYREENDYFFVYDKNSTILKLPLTPERDGTKNNGK
                     HILEFIKTAFEKGEGFVPYEQVIPGKEPRAKLSNIRLFEPYGWVVGIGVYIDNEEKEL
                     KAKALNEISKIRFGQDGYFFVYDYDGTNIMHPINPSLVGKNLIENKSQKGVYYIKDLI
                     EAAKKGGGTVIFDFPKSKDDPTLYDKIGYADGLQEWKWMIGTGVYVDNIEKNIEIMHQ
                     NSKEKITSIILGIVIIAIVVSVILIFIISFLSQKR"
     gene            complement(1457..1776)
                     /gene="rnpB"
                     /locus_tag="PKO10_04435"
     ncRNA           complement(1457..1776)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PKO10_04435"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT          574 a          304 c          209 g          763 t
ORIGIN      
        1 atcattatct gtctttgcaa taattctctc ttttagattt cctttattta tttgcgaaac
       61 aacctctttt acatttttaa ttaatgcttc atcttgtttt aatagattgt ttgttctttc
      121 aatattttca tttactactt ttgttattat tcctatttca tcttcagatt ttatctctat
      181 tttagaaaca tctgaactct ctttatttag atatttaaaa aatgataaaa gtccattttc
      241 aaatctttct aaaggagaga ttatctcttt tgtgataaaa atgagataat aaaaattaaa
      301 ataactgaaa caactatagc aataataact attcctaaaa taattgaagt aattttctct
      361 tttgagtttt gatgcataat ttcaatgttt ttttcaatat tatcaacata aactccagtt
      421 ccaatcatcc atttccactc ttgaagacca tcagcatacc ctattttgtc atataaagtt
      481 ggatcatctt tactttttgg gaaatcaaaa attactgttc caccgccttt ttttgcagct
      541 tctatcaaat cttttatata ataaacacct ttttggcttt tattttcaat taaatttttt
      601 ccaactaatg atggatttat tggatgcatt atgtttgtac catcataatc atacacaaaa
      661 aaatatccat cttgaccaaa tctaattttt gaaatttcat ttaaagcttt tgcttttaac
      721 tctttttctt cattatcaat ataaactcct attccaacga cccaaccata aggctcaaat
      781 aatcttatat tagatagttt tgctctaggt tctttacctg ggattacctg ttcgtaagga
      841 acgaaacctt cacctttttc aaaagcagtt tttataaatt ctaaaatatg ttttccattg
      901 tttttagtac cgtctctctc tggtgttaaa ggtaatttta gtattgtaga atttttatca
      961 tagacaaaga aatagtcatt ttcctctcta tatctaacag aaccaatagc ttctaaaaga
     1021 atttttttta gttctgcttc tggcatttta tttttttgtt catcataaat tttagatatc
     1081 attgcaaata aaaaatcagt ttgtgacttt aaataggcac cttttcttgc tttgatattt
     1141 tcaatttttg cttcttcata agcacttttt gcaatatttt gagcaaatgc tgtatagttt
     1201 tttaactctt ctatacttgc attaaaagca ttttgcctat actcttcaat agtttgattt
     1261 gtaaggttat taacctcata aatagacttt gtagctatta tagtagagac taatataata
     1321 gtaattatta cgatacttag taacttgact cgtattgata aatttttcat aaaaacccct
     1381 tttgtgtttg ttgaaaatca ttttaccaaa agaattataa gtttgtttgt aattttatat
     1441 agatttttat tatattttta aaaggtagat tataagccgt gttttgtatt tgacaataat
     1501 ttatctaggt attaaatcac tttaatactc atgcgaagac gaaaaatgtg aagctaaata
     1561 ccatgtcttc ttgctgcaag ttgggtttac aaagctgtta agattactca aaacactggt
     1621 aggctcttac tctaccgttt caccatcacc aaaaattggc tgtatacttt ctgttgcact
     1681 atcccttaga ttactctagc catccgttag atggaacctt gcttcactgc agcacggact
     1741 ttcctcttga aatcaagcta ttgtctatct acctttggtc ggaattgtat ctattttctt
     1801 attaaatcat aattactagg agctacaaat acaaaaatat tatcatcaat
//
LOCUS       DAOQAN010000239         1849 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_95221_length_1849_cov_3.419732, whole genome shotgun sequence.
ACCESSION   DAOQAN010000239 DAOQAN010000000
VERSION     DAOQAN010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95221_length_1849_cov_3.419732"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="PKO10_04440"
     CDS             complement(<1..675)
                     /locus_tag="PKO10_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014469141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HNW66429.1"
                     /translation="MKSIKKIIFLKSLTAIFCIGLFAFNLENNYISNEKHSKLLLKPI
                     DGLNNEQTDIFMLGRSFFNIPWVKAPSVTTARDGLGPLFNANSCISCHPKNGRGELFT
                     KDNLTSRALIARLSIKSDDSFQHKDILKKKGFIPEPVYGEQLAINGNLKVPFEGNIKI
                     DFEDFEVKFPDGETVILQKPKYSLENLNYGELKENSVISYRLAQSLNGMALIDLIDEK
                     EILKNQD"
     gene            complement(676..>1849)
                     /locus_tag="PKO10_04445"
     CDS             complement(676..>1849)
                     /locus_tag="PKO10_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013134181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="imelysin family protein"
                     /protein_id="HNW66430.1"
                     /translation="YFHQNSIPIHASNPTQENLDSAKKAWLQARESYGSTEIFRLANG
                     PIDSEDGWIAETYGALEGQINAWPLDENMIDYTIDADGKRTSGNIIDTIGKFNPGGEE
                     SKEVDVTKITVEALTELNENGGEANVSTGYHAIEFLLWGQDQDYNNFLEDKITNGAMV
                     AGLRPLSDFTTDKDAKRRLEYLSVVTQKLVEDLSVVTSAWEKDIKGNAGLYRAALLGK
                     LKGKEKDKNLDKKETMKQIIAGMGVFIKSELANERVAISVLTPSEEDEHSCFSDNTHR
                     DLVKNYEGFKNILTSTYNGKKYGESLLDSLNKDDKNRVLKLMSDIEEKIDSVDKIAKT
                     QAHFDYQIKPEHPQAKVLVKLKNELRKLGDEMITVAKANNIKLSTDDVTDPEETKL"
BASE COUNT          558 a          332 c          218 g          741 t
ORIGIN      
        1 atcttggttt tttaaaatct ctttttcatc tattaaatct atcaaagcca taccatttaa
       61 actttgagct agcctatatg aaattacact attttctttg agttccccat aatttagatt
      121 ttctaaacta tattttggtt tttgtaaaat cactgtttca ccatctggga atttaacttc
      181 aaaatcttca aaatctattt taatatttcc ttcaaaagga actttcaaat taccattaat
      241 agctaattgt tctccataaa caggctctgg aataaatcct tttttcttta aaatatcttt
      301 gtgctgaaaa ctatcatctg attttattga caatcttgca attaaagctc ttgaagttaa
      361 attatcttta gtaaaaagtt ctccacgacc attttttggg tgacaagaga tacaactatt
      421 tgcattaaaa agtggtccta atccatctcg tgcagttgta acactaggtg cttttaccca
      481 aggaatatta aaaaagcttc ttcctaacat aaaaatatca gtttgttcat tatttaaacc
      541 atcaattggt tttaaaagta gcttactatg tttttcattt gatatataat tattttcaag
      601 attaaatgca aaaagcccta tgcaaaatat tgcagtaagg ctttttaaaa atataatctt
      661 ttttatagat ttcatttaca gttttgtctc ttctggatct gtaacatcat ctgttgatag
      721 tttgatatta tttgcttttg caacagttat catctcatca cctaattttc ttagctcatt
      781 tttaagtttt actaaaactt ttgcttgtgg atgctctggt ttgatttggt agtcaaagtg
      841 agcttgcgtt tttgcaattt tatctacact atctattttt tcttcaatat cactcataag
      901 ttttaaaact ctgtttttat catctttatt tagtgaatca agtagagatt ctccatattt
      961 tttaccatta tatgtagaag ttaaaatatt tttaaatcct tcatagtttt ttactaaatc
     1021 tctatgagta ttatcagaga agcaagagtg ctcatcttct tcgctaggag ttaaaacaga
     1081 aatagcaact ctttcgtttg caagttcaga ttttataaat acacccattc cagcaataat
     1141 ttgtttcatt gtctcttttt tatctaaatt tttatctttt tctttacctt ttaatttacc
     1201 taaaagagcc gctctataaa gtccagcatt acctttaata tctttttccc aagcacttgt
     1261 aacaacactt aaatcttcaa ctaatttttg tgtaacaaca cttaaatact ctaatcttct
     1321 ttttgcatct ttgtctgttg taaaatcact aagtggtctt aaaccagcta ccattgcgcc
     1381 gtttgtaatt ttatcttcta agaagttatt ataatcttga tcttgtcccc aaagcaagaa
     1441 ctcaatagca tggtaaccag ttgaaacatt tgcttctcca ccattttcat taagttctgt
     1501 taaagcttca actgtaattt ttgtaacatc aacttctttt gattcttcac cacctggatt
     1561 aaactttcca atagtgtcaa taatatttcc agatgttctt tttccatctg catcaatagt
     1621 ataatcaatc atattttcat caagtggcca agcattaatt tgtccctcta aagcaccata
     1681 agtttctgct atccaaccat cttcagaatc aattggacca tttgcaagcc taaaaatttc
     1741 agtacttcca tatgactctc ttgcttgtaa ccaagctttt tttgcactat caagattttc
     1801 ttgagttgga tttgaagcgt ggatcggaat actattctga tgaaagtaa
//
LOCUS       DAOQAN010000240         1849 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_95254_length_1849_cov_2.319955, whole genome shotgun sequence.
ACCESSION   DAOQAN010000240 DAOQAN010000000
VERSION     DAOQAN010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95254_length_1849_cov_2.319955"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..1423)
                     /locus_tag="PKO10_04450"
     CDS             complement(56..1423)
                     /locus_tag="PKO10_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HNW66431.1"
                     /translation="MATRSDFFSKEYLDELKELHDKLPKMSKSDFEDVFKESFKQECF
                     KKFENEPIASASIGQVHIAYLQDDTKVAVKLRRKNIEKQVRVDIKILNFFNKLFRPLF
                     SYYTKNSIDAVINEFSSMIKDETNLTIELENLKKFSATYKDSGVLFPKPFEEFCSNSA
                     IVMSFMDGFRFDDKTSLEKYDIDFKQIISKLVNFYTEQMLINGYFHADPHPGNLLVNA
                     NGELILLDFGMVKNIPNDSRVAIIELIDGANRGDFETFVRANKKLGTISYEAPEALMA
                     QFSQKMFDIFSNDNLSSESMQKLAFEVLESTRDLPFKLPSDAVYILRVSAIIEGLGTT
                     YIENFNGVKDILPILKDNLPKALGVKTTISQIVLDQLESFPKFIKNLKQLVAKGSKGE
                     LEVQINKEQLEFIKKDLKEYFSSYLKSLSFILFGIFLIFYDESLKNLALILVSFGFLR
                     GIFIK"
     gene            complement(1612..>1849)
                     /locus_tag="PKO10_04455"
     CDS             complement(1612..>1849)
                     /locus_tag="PKO10_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66432.1"
                     /translation="LGGALLLKERVEEELKTLQEKGKIKTSDAKSFLESLEQKGKDED
                     ERIKAKIKDMFKEVLDELGVATKADLEKLKEDLK"
BASE COUNT          626 a          300 c          209 g          714 t
ORIGIN      
        1 tctactaatt ttaatttcct tttaaattat atttaaaaaa ttacttttta aatatttact
       61 ttataaaaat acctcttaaa aacccaaaag agaccaaaat caaagcaaga ttttttaaac
      121 tctcatcata aaagattaaa aatattccaa acaaaataaa agatagagat tttaagtatg
      181 aactaaaata ctcttttaaa tcttttttta taaactctag ttgctctttg ttaatttgta
      241 cttcaagttc accttttgaa ccttttgcaa ctagttgttt tagatttttt ataaatttag
      301 gaaatgactc taactgatct aaaactattt gtgaaattgt agttttaact cctaaagctt
      361 ttggaagatt atcttttaaa attggtaaaa tatcttttac accattaaag ttttctatat
      421 aagttgtgcc taaaccctct attattgcac taactcttaa aatataaaca gcatcgcttg
      481 gaagtttaaa tggtaaatct cttgtacttt ccaaaacttc aaatgctagt ttttgcatag
      541 attcactaga taaattatcg tttgaaaaga tgtcaaacat cttttgacta aactgggcca
      601 tcaaagcttc aggtgcttca tagctaatag ttccaagttt tttgtttgct cttacaaaag
      661 tttcaaaatc ccctctattt gcaccatcaa ttagctcaat tatcgcaact cttgaatcat
      721 ttggtatatt tttaaccatt ccaaaatcaa gtaaaattag ctctccattt gcgtttacaa
      781 gcaagtttcc agggtgaggg tctgcatgaa aatatccatt tattaacatc tgctctgtgt
      841 agaagtttac aagttttgag ataatttgtt taaaatcaat atcatatttt tctaaagaag
      901 ttttatcatc aaatctaaaa ccatccataa aactcataac tatggcacta tttgagcaaa
      961 actcttcaaa aggttttgga aataaaactc cactatcttt atatgtagca gagaactttt
     1021 ttaaattttc aagctctatt gtaaggtttg tctcatcttt tatcatagat gaaaactcat
     1081 ttataacagc atctattgaa ttttttgtgt aatatgaaaa aagtggtcta aaaagtttgt
     1141 tgaagaaatt taatattttt atatcaactc ttacttgttt ttcaatattt tttcttctta
     1201 atttaactgc aacttttgta tcatcttgca aatatgcaat atgaacttga ccaatagaag
     1261 cactagctat tggctcattt tcaaactttt taaaacactc ttgcttgaaa gactctttaa
     1321 aaacatcttc aaaatcagat ttagacattt ttggaagttt gtcgtgtagc tcttttagct
     1381 catctaaata ctcttttgaa aaaaaatcag atctagttgc caaaacttgt gcaagtttta
     1441 taaaactagc tcccaaataa aatattgtct cttttagttt ttttggcttt aatggtttaa
     1501 ggaacaaaaa actatccctt tttttaataa gtaaaaaaag agatagtaag aatctaaata
     1561 ttaaatagac tctaaaaggt gagaaaagtt taagatagat ataaaaagtt tttattttaa
     1621 atcctctttt aacttctcta aatcagcttt tgtagcaact cctagttcgt ctaaaacctc
     1681 tttaaacata tcttttatct tagctttaat tcgctcatct tcatcttttc ctttttgttc
     1741 taaagattcc aaaaagctct ttgcatcgct agttttgatt tttcctttct cctgtagagt
     1801 ttttaactcc tcttcaactc gctcttttaa aagtaacgct ccaccaagt
//
LOCUS       DAOQAN010000241         1848 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_95309_length_1848_cov_2.511433, whole genome shotgun sequence.
ACCESSION   DAOQAN010000241 DAOQAN010000000
VERSION     DAOQAN010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95309_length_1848_cov_2.511433"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1555)
                     /gene="pckA"
                     /locus_tag="PKO10_04460"
     CDS             complement(<1..1555)
                     /gene="pckA"
                     /locus_tag="PKO10_04460"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HNW66433.1"
                     /translation="MSNIKDSLGLENIGTIFRNSDVDFLIDFAVKNEKAKISSTGALM
                     IDTGIFTGRSPKDKFFVNQDPSNKYIAWGDINRKVSKEVYVDLLANSKKQLSNKDIFV
                     TDVFCGSSLDSRRAVRFITEVAWQAHFIQNMFILPTKEELENFKPDFTIYNACKTVDM
                     AYVSHGLHSEVYVIFNVEENQAIIGGTWYAGEMKKGVFSMMNYWLPLEGKLPMHCSAN
                     IGKDGDTALFFGLSGTGKTTLSTDPNRALIGDDEHGWDDNGVFNFEGGCYAKVINLDP
                     ESEPEIFGAIKKGAILENVVADENGVVDYSDKSKTENTRVSYPIDHIENHAKTLRGGH
                     PKNIIFLCADAFGVLPPVAKLDKQQAMYYFLSGYTAKVAGTERGINEPMATFSSCFGE
                     AFLPLNPTVYAELLGKKIDEHNVNVFLVNTGWTGGPYGVGKRMSIKNTRACINAILDG
                     SINESEFQNMTIFNIQIPKTLKGVDTHVLTPRYTWSDPLEYDKAKRKLAEMYIENFKK
                     YLTLESEYDF"
BASE COUNT          632 a          314 c          236 g          666 t
ORIGIN      
        1 taaaatcata ttcactctca agagttaaat attttttaaa gttttctata tacatctctg
       61 ctaattttct ttttgctttg tcatactcta atgggtcaga ccatgtatat ctaggagtta
      121 atacatgagt atctacacct tttagtgttt ttggaatttg tatattaaat attgtcatat
      181 tttggaattc agattcattt atagaaccat ctaaaatagc atttatacaa gctcttgtat
      241 ttttaatact cattcttttt ccaacaccat aaggaccacc tgtccatcct gtgtttacta
      301 aaaatacatt tacattgtgt tcatctattt ttttacctaa aagctcagca taaacagttg
      361 ggtttagtgg taaaaaggcc tctccaaaac aagaagagaa agttgccatt ggctcgttta
      421 ttcctctttc agttcctgct acttttgcag tataaccact taagaagtag tacatagctt
      481 gttgtttatc tagttttgca actggaggta aaacaccaaa agcatcagca cataagaata
      541 taatattttt tggatgccca cctcttaaag tttttgcatg attttctata tgatctattg
      601 ggtaagaaac tcttgtattt tctgtttttg atttatcaga gtaatctaca actccatttt
      661 catctgcaac aacattttct aaaatagcac cttttttaat agcaccaaag atttcaggtt
      721 cgctttctgg gtcaaggtta attacttttg cataacaacc accctcaaag ttaaatactc
      781 cgttatcatc ccaaccatgc tcatcatcac caattaatgc tctatttgga tctgttgaaa
      841 gcgtagtttt tcctgttcca gaaagtccaa agaatagtgc agtgtcacca tcttttccaa
      901 tatttgcaga acagtgcatt ggaagttttc cttctagtgg aagccagtag ttcatcatag
      961 agaacacacc ttttttcatc tctccagcat accaagttcc accaataata gcttgattct
     1021 cttccacatt aaatataaca taaacttctg agtgtaaacc atgacttaca taagccatat
     1081 caacagtttt acatgcattg taaattgtaa aatcaggttt aaagttttca agctcctctt
     1141 ttgtaggaag gataaacata ttttgaataa aatgagcttg ccaagcaact tcagtaataa
     1201 atcttactgc tcttcttgaa tctaatgaag aaccacagaa aacatctgtt acaaaaatat
     1261 ctttgttgct tagttgtttt tttgagtttg caagtagatc aacataaacc tcttttgaaa
     1321 cttttctatt tatatctccc caagcaatat atttatttga tgggtcttga tttacaaaga
     1381 acttatcttt tggacttcta cctgtaaaaa tacctgtatc aatcattaaa gcaccagtag
     1441 aagatatttt tgccttctca ttttttaccg caaaatcgat taaaaaatcg acatccgaat
     1501 ttctaaatat cgtaccaatg ttttctaaac ctaatgagtc tttaatatta gacataattc
     1561 aaattcctat tctaattttt aatgcgaaat tttatccaaa aaaatataaa actttctgaa
     1621 agatgagtaa atttcataac aaattgaata tttcttgttt tttataaaaa aatataaaaa
     1681 aaagcaaaaa aatagttgaa aaatatttca aaatgtaata taatttcaaa aataattcaa
     1741 aagaaggaat aaaatgttta tagttgaaga tattcttaat aagttaaaaa atattttaaa
     1801 aactgatagt aaaacaaaaa aagtttttga taaagagtta gctgaggc
//
LOCUS       DAOQAN010000242         1844 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_95671_length_1844_cov_2.277250, whole genome shotgun sequence.
ACCESSION   DAOQAN010000242 DAOQAN010000000
VERSION     DAOQAN010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95671_length_1844_cov_2.277250"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="PKO10_04465"
     CDS             complement(<1..440)
                     /locus_tag="PKO10_04465"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HNW66434.1"
                     /translation="MIGALWTGISGLAAHQTALDNESNNIANVNTVGYKAGRISFADQ
                     IYQGKIGKGSFVQDAEKIFVTGGSKITGVEYDVALQGDGFFTVINKNTLGTAETFYTR
                     AGNFRMGQSGTLQNPDGYEVQGWTMSSIDQKNDVKSTNQNATRFT"
     gene            complement(551..>1844)
                     /locus_tag="PKO10_04470"
     CDS             complement(551..>1844)
                     /locus_tag="PKO10_04470"
                     /inference="COORDINATES: protein motif:HMM:NF018168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar basal body rod C-terminal
                     domain-containing protein"
                     /protein_id="HNW66435.1"
                     /translation="TTTTNPLDMMFTDEYTKSMVSTNINSGSGTVYNINAKASDYVSS
                     SKDDDILKKGDGYKTSQNKINDIEALKADYIEKLNKFLMDQSTTNTPSISQKSQIDFS
                     SNLSSLQATDNTISVTIDNKTYSVKFDVNSTINDEEMQKLYDFLDTNGKAKYNLVDPN
                     SIQSQANIDAMPTTTPQEILDKATAQALRDDQINSYTNANSIINAMKDLSDKISSKEG
                     MSSSVKDGTLVIDTLIAGGSFNISDIKLNDTNFSSTKLQEAVKGSGLAMVDSARDALK
                     NAVENADGKFLQITNVLEYGNLGVIGENDINVRLDELGISDKSTADISISDDGFVFVK
                     SQGHSFLVGRLSTAGFRNEQGLEPMGGNLFQQSQYSGNPFNSDTMNIIRGGALERANI
                     DYGSTLTQIMVYQKAFEASSKSITTSDEFLQTAIQMKK"
BASE COUNT          551 a          332 c          228 g          733 t
ORIGIN      
        1 gtaaatcttg tagcattttg atttgtactc tttacatcat ttttttggtc aattgaactc
       61 atagtccaac cttgaacctc atatccatct gggttttgta aagttccact ttgacccata
      121 cggaaatttc cagctcttgt ataaaatgtt tctgcagttc ctagtgtatt tttatttatt
      181 acagtgaaaa atccatcacc ttgtaaagca acatcatact caactcctgt gatttttgaa
      241 ccacctgtta cgaaaatctt ctctgcgtct tgcacaaatg atcctttacc aatctttcct
      301 tgataaattt gatctgcaaa agagattctt cccgctttat aacctacagt gtttacattg
      361 gcaatgttgt ttgactcatt atccaatgct gtttgatgtg ctgctaatcc tgatattcca
      421 gtccataatg cacctatcat gactaatcct ttgtattaag gtaaatgaac ataaatgttc
      481 atacttttgt acaaaaatta tattaaattt atacaaaagt ataaacacaa agtatttata
      541 ctaattttta ctatttcttc atctgtattg ctgtttgtaa aaactcatct gatgttgtta
      601 tagattttga actagcttca aaagcttttt gataaaccat aatttgagtt aaagtagaac
      661 cataatctat gttagctctt tcaagcgcac cacctcttat tatattcata gtatctgagt
      721 taaaaggatt tccactatat tgtgactgtt gaaatagatt tccacccata ggctctaatc
      781 cttgttcatt tctaaatcca gcagtgctaa gtcttcctac taaaaaacta tgaccttgac
      841 tttttacaaa aacaaaacca tcatctgata tacttatatc ggctgtactt ttatcagaaa
      901 ttcctagctc atcaagtctt acatttatat cattttctcc aataactcct aagtttccat
      961 actccaatac atttgtaatt tgtaaaaact ttccatctgc attttcaact gcatttttta
     1021 aagcatctct tgcactatca accatagcaa gaccagaacc ttttaccgcc tcttgtaatt
     1081 ttgtagaact aaagtttgta tcatttaact ttatatctga tatattaaag ctaccaccag
     1141 caattaaagt atcaataacc aaagttccat cttttacact agaactcata ccctcttttg
     1201 aagaaatttt atcagataaa tctttcatag catttattat actatttgca tttgtgtatg
     1261 aattgatttg atcatctctt aaagcttgtg ctgttgcttt atctaaaatc tcttggggag
     1321 ttgttgtcgg cattgcatct atgtttgctt ggctttgtat agagtttgga tcaactaaat
     1381 tatactttgc ttttccattt gtatctaaaa agtcatatag cttttgcatc tcttcatcat
     1441 ttatagttga gtttacatca aatttcacac tatatgtttt attgtcaatt gttacactaa
     1501 tagtattatc tgtagcttgt aaagatgata aatttgatga aaaatctatt tgagattttt
     1561 gactaataga tggagtattt gttgttgatt gatccatcaa aaatttattt aacttctcta
     1621 tatagtcagc ttttaatgct tcaatatcat ttatcttatt ttgtgaagtt ttgtatccat
     1681 cacctttttt taatatatca tcatcttttg aagagcttac ataatcacta gcttttgcat
     1741 ttatgttata aactgtaccg cttccactat ttatatttgt agataccatg ctttttgtat
     1801 attcatcagt aaacatcata tcaagtggat ttgtagttgt agta
//
LOCUS       DAOQAN010000243         1843 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_95724_length_1843_cov_3.041387, whole genome shotgun sequence.
ACCESSION   DAOQAN010000243 DAOQAN010000000
VERSION     DAOQAN010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95724_length_1843_cov_3.041387"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1843
                     /locus_tag="PKO10_04475"
     CDS             <1..>1843
                     /locus_tag="PKO10_04475"
                     /inference="COORDINATES: protein motif:HMM:NF024977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNW66436.1"
                     /translation="IFLASSNRVILNLNKTACEIFGYTKEELLNQSFERIHLSKESFD
                     KFSLEYKKFENSILTNCEFPFKHKNGNTIFCSIFGTPLDNSDLSKGFIWTLLDITEKK
                     LAEKALIKEKNLFSSGPVVVAEWKFDKNWPVKYISSNCETVLGYTKDEMLSEDFNYSS
                     IIHKDDLERVSKEISFYTKDRNSYEQSYRIRLKNGEYRWFYDFNQVIKNSSGEIEAIR
                     GYMFDQTQLKESEIILQKMNEELKLQTDIAQKANESKNQFLANMSHEMRTPMNAIIGL
                     SELLADTKIDEKQYDFIKKINTSSKLLLGIISDILDFSKIEAGNFDLDLDLKAFSLEN
                     LLSQLRAIFYQVENYKNIELYFYNKSGNPKIVLSDELKILQVLTNFISNALKFTNKGN
                     ITLKIELLEKISENSAKIRFSVQDTGIGISEDDIPNLFKPFFQVDNSATRKYGGTGLG
                     LSISERIVNSLGSTIEVQSQKDVGTEFSFTINMEVLEWSKENLLNSKKFKILIVDDQE
                     ISRVILKDILENFDYVTYEAKDGIESIEMVKEADISNEPFDFILMDFNMPRLNGKEAI
                     KKIYELYNQKEIKKNVPSILMVSAYSKDEINLDDVHIDNFLTKPITSS"
BASE COUNT          740 a          190 c          299 g          614 t
ORIGIN      
        1 gtatattctt agcttcatct aatagagtta tcttaaattt aaataaaact gcttgtgaaa
       61 tttttggtta tacaaaagag gagctactta atcaaagttt tgaaagaatt cacctttcaa
      121 aagagtcttt tgataaattc tcactagagt ataaaaaatt tgaaaactct attttaacaa
      181 attgtgagtt tccttttaaa cacaaaaatg ggaatactat tttttgctct atttttggga
      241 cacctcttga taatagtgat ttatcaaaag gatttatttg gacactatta gatattacag
      301 agaaaaaact tgcagaaaaa gctttgataa aagagaaaaa tcttttttct agtggtccag
      361 ttgtggtagc tgagtggaaa tttgataaaa attggcctgt gaaatatata tcatcgaact
      421 gtgaaacagt tttaggatat acaaaagatg agatgctttc agaagatttt aattattcat
      481 caataattca taaagatgat ttagaaagag tttcaaaaga gattagtttt tatacaaaag
      541 atagaaactc ttatgagcag tcttatagaa ttcgtcttaa aaatggagag tatcgttggt
      601 tttatgattt taaccaagta ataaaaaact cttctggaga gattgaagct ataagaggat
      661 atatgtttga tcaaactcaa ttaaaagaga gtgaaataat attacaaaaa atgaatgaag
      721 agctaaaact tcaaaccgat attgcacaaa aagcaaatga gtcaaaaaat cagtttttgg
      781 caaatatgag tcatgaaatg agaaccccta tgaatgcaat tataggttta agtgaacttt
      841 tggcggatac aaaaattgat gaaaaacagt atgattttat aaaaaaaata aatacttctt
      901 ctaaactact tttaggaatt atcagtgata ttttagattt ttcaaaaatt gaagcaggta
      961 attttgattt agatttagat ttaaaagctt tctctttgga gaatttatta tctcaattaa
     1021 gagcaatttt ttatcaagtt gagaattata aaaatattga actatatttt tataataaat
     1081 cgggaaatcc aaaaattgtt ttaagtgatg agttaaaaat attgcaagtt ctaacaaatt
     1141 tcataagcaa tgctctaaaa tttacaaata agggaaatat tactttaaag atagaacttc
     1201 ttgaaaaaat atctgaaaat agcgcaaaaa taagatttag tgttcaagat acaggaattg
     1261 gaattagtga agatgacatt ccaaatctat ttaaaccttt ttttcaagta gataactctg
     1321 caactagaaa atatggggga actggattgg gattatcaat ctcggaaaga atagtaaact
     1381 ctttgggtag cacaatagag gtacaaagtc aaaaagatgt aggaacggaa tttagtttta
     1441 ctataaatat ggaagtttta gagtggtcaa aagaaaactt actaaatagt aaaaaattta
     1501 aaattttaat agttgatgat caagaaattt caagagtaat attaaaagat attctagaaa
     1561 attttgatta tgttacttat gaggcaaaag atggaattga atcaatagag atggtaaaag
     1621 aagctgatat ttcaaacgaa ccttttgatt ttattcttat ggattttaat atgcctaggc
     1681 taaatgggaa ggaggcaatt aaaaaaattt atgaattgta taatcaaaaa gagattaaaa
     1741 aaaatgttcc ttctatttta atggtttcag cttactcaaa agatgagata aatttagatg
     1801 atgtccatat tgataatttc ttaactaaac ctattacgag ttc
//
LOCUS       DAOQAN010000244         1837 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_96174_length_1837_cov_6.913580, whole genome shotgun sequence.
ACCESSION   DAOQAN010000244 DAOQAN010000000
VERSION     DAOQAN010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96174_length_1837_cov_6.913580"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(33..251)
                     /locus_tag="PKO10_04480"
     CDS             complement(33..251)
                     /locus_tag="PKO10_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66437.1"
                     /translation="MRNNTKEGITNQKIEYESFEIVSTFFVSTYIQIKANTDTKGIDA
                     KIAPINELLFESSDIPTIKIAEIKIFIM"
     gene            complement(248..1612)
                     /locus_tag="PKO10_04485"
     CDS             complement(248..1612)
                     /locus_tag="PKO10_04485"
                     /inference="COORDINATES: protein motif:HMM:NF016571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNW66438.1"
                     /translation="MELANYIETAMKSILKNPIYEVLSEIKITKILKQSNFVKREVGY
                     PPFQIILHFLYMLIMQKRQSTFIKNSDKAYGKDVYYRFIKEKRYNWRKLLLLSATEIL
                     KKIKPLHKNGEYRLLIIDDTVEPKRGKFIEGTCKYIWSNKEHRSINALNIVSLNYADS
                     HSTFQLDFSIKMNDSKRKSTSEFTAKLHHRSNAYQRKSEIIKGKNILALEMLERALDN
                     GIDADYLLVDSWYAKPNFIKEANTLGMPVIARLPNNKLIWNFKGKYKTLNAIYDSFKN
                     IRHKYSGKHGKISYKYFDSVLEHAVLGKVKLVFLHTGKDLLVFISTDISISGKEILET
                     YKKRWNIEQGYKDLRLLFGLGKEENRIYEALIGKITLSMFTYNIVSYINRIKHEPQTL
                     GELFRDLECELETLAISMQLFIQILTKISEIQNVVKDNKDLLQIIAVISAFTQKELGF
                     MCES"
BASE COUNT          587 a          268 c          233 g          749 t
ORIGIN      
        1 ctatttaaaa taaaaaaggc tttttaaatg tattacatta taaaaatttt aatttctgct
       61 attttaattg tgggtatatc agaactttca aaaagaagtt cattgattgg agctatttta
      121 gcatctatac cttttgtatc cgtgttggct tttatttgga tatatgttga tacaaaaaat
      181 gtagaaacta tctcaaaact ttcatattca attttttggt tagttattcc ctctttagta
      241 ttatttctca actttcgcac ataaaaccta actctttttg agtgaaagca cttatcactg
      301 caataatttg aagtaaatct ttattatcct tgacaacatt ttggatttca gagatttttg
      361 taagtatttg aataaaaagt tgcattgaaa ttgcaagggt ttcaagttca cactctaaat
      421 ctctaaaaag ttctcctagt gtttgtggct catgttttat gcggtttatg taacttacaa
      481 tgttgtaagt aaacatagaa agagtgattt ttccaataag agcttcatag atacgatttt
      541 cttcttttcc taagccaaac aagagtctta gatctttata gccttgttca atattccatc
      601 tctttttata agtttctaaa atctctttac ctgaaatact tatatctgtt gatataaaaa
      661 ctaataaatc ttttccagtg tgtaaaaata ccaatttaac ttttcctaaa acagcatgtt
      721 ctaaaactga atcgaaatac ttataagata tctttccatg tttaccgcta tatttatgac
      781 gaatattttt aaaactatca tatattgcat ttagagtttt atatttacct ttaaagttcc
      841 aaatgagctt attatttgga agtcttgcaa ttacaggcat tcctaaagta tttgcttctt
      901 ttataaaatt tggtttagcg taccagctat caacaagtag ataatcagca tcaattccat
      961 tatctaatgc tctttctaac atctcaagag ctagtatatt cttaccttta ataatttcac
     1021 tcttacgctg atatgcgtta cttctatgat gcaattttgc tgtgaactct gatgtagatt
     1081 ttcttttact atcattcatt tttatagaaa aatccaattg aaatgttgag tgtgaatctg
     1141 cataatttag agacactata tttagtgcat taatacttct atgctcttta ttactccaga
     1201 tatatttaca agtaccttca ataaatttac ctctttttgg ttctactgta tcatcgataa
     1261 ttagtaggcg atactcacca tttttatgca atggctttat cttttttaaa atttctgtag
     1321 cacttaacaa taaaagttta cgccagttat agcgtttttc tttgataaat ctataatata
     1381 catctttacc ataagcctta tcactatttt ttataaaagt tgattgtctt ttttgcataa
     1441 taagcatata aagaaagtgt aaaattattt gaaatggtgg atatccaact tctctcttaa
     1501 caaagttact ttgtttaagt atttttgtga tttttatttc tgataacact tcgtaaattg
     1561 gattttttaa tatgctcttc atagcagttt ctatataatt tgcaagttcc ataagataga
     1621 cctttataat gatgttagtg gtaacgtaat tatactaaaa agtgcctact tatggacttt
     1681 atttatcaac tttagttcat gaatttattg gaatctatgt gcgaaagttg agttatataa
     1741 taaaaaaatt tatttaggag actttatgaa aaaaacagtt ttgctgtcat caatcttatt
     1801 aacatctatt tttgcttttg atttaaagag tattgca
//
LOCUS       DAOQAN010000245         1832 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_96653_length_1832_cov_2.402926, whole genome shotgun sequence.
ACCESSION   DAOQAN010000245 DAOQAN010000000
VERSION     DAOQAN010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96653_length_1832_cov_2.402926"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1584
                     /gene="ftsH"
                     /locus_tag="PKO10_04490"
     CDS             <1..1584
                     /gene="ftsH"
                     /locus_tag="PKO10_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013327528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HNW66439.1"
                     /translation="LFFAIWMFLVKRMQKSMGGGSGGILGIGSSKKMINSEKPNVKFD
                     DMAGNKEAKEEVREVVDFLKAPDRYVRLGAQIPKGVLLVGPPGSGKTLLAKAVAGEAD
                     VQFLSVSGSAFIEMFVGVGASRVRDLFEQAKKVAPAIIFIDEIDAIGKSRASGGPMGG
                     NDEREQTLNQLLAEMDGFSTETAPVIVLAATNRPEVLDPALLRPGRFDRQVLVDKPDY
                     EGRIEILNVHIKDVKISKGVDLKEVAKMTAGLAGADLANIINEAALLAGRANKDEVEP
                     SDFKEAVERQIAGLEKKSRRISPKERRIVAYHESGHAVMAEITKGAKKVNKVSIVPRG
                     LAALGYTLNTPEENKYLMQKHELIAEVDVLLGGRAAEEVFIGEISTGAGNDLERATNI
                     IKSMATIYGMSDIAGLMVLERRTNQFLGGQTQKDFSDAMAKDLDDHVKTTLNERYQVV
                     LQALRDNKDAIEQMTSELLEIEVITGERVREIIKEHGGIVFEGEDLHSDSLDKSDLKE
                     DIKSDKTQIENKDDNQSEK"
BASE COUNT          695 a          201 c          369 g          567 t
ORIGIN      
        1 ttattttttg ctatttggat gtttttggtt aaaagaatgc aaaaatctat gggtggtgga
       61 agtggtggaa tccttggaat tggttcatct aaaaagatga taaattctga aaaaccaaat
      121 gtaaaatttg atgatatggc tggaaataaa gaggctaaag aagaggttag agaagttgta
      181 gatttcttaa aagcaccaga tagatatgta agacttggag ctcaaattcc aaaaggtgta
      241 ttacttgtag gtcctccagg ctcaggtaaa actcttttag caaaagcagt tgctggagag
      301 gctgatgttc aatttttatc tgtttcaggg tctgcattta ttgagatgtt tgtaggagta
      361 ggtgcaagta gagttagaga tttatttgaa caagcaaaaa aagtagctcc tgctattatt
      421 tttattgatg agattgatgc tattggtaaa agcagagcct ctggcggacc aatgggtgga
      481 aatgatgaga gagagcagac tttaaatcaa cttttagcag agatggatgg attttctact
      541 gaaacagctc ctgtaatagt tcttgcagct acaaatagac ctgaagtttt agatccagca
      601 cttttaagac caggaagatt tgatagacaa gttttggttg ataaacctga ttatgaagga
      661 agaattgaga ttttaaatgt tcatattaaa gatgtaaaaa tttcaaaagg tgtagattta
      721 aaagaggttg caaagatgac agctggactt gctggtgctg atttagcaaa tattataaat
      781 gaagctgctc ttttagctgg acgtgcaaat aaagatgaag tagagccaag tgattttaaa
      841 gaggctgttg agagacaaat agctggactt gaaaaaaaat caagaagaat atctccaaaa
      901 gaaagacgta tagttgctta tcatgaaagt ggacatgcgg ttatggctga aattacaaaa
      961 ggtgctaaaa aagtaaataa agtatctatt gttccaagag gtttagctgc acttggatat
     1021 acactaaata ctcctgaaga gaataaatat ttaatgcaaa aacatgagct aatagctgaa
     1081 gttgatgttt tattaggtgg aagagctgct gaagaggtat ttataggaga gattagtacg
     1141 ggtgctggaa atgatttaga gagagctaca aatattatca aatctatggc aactatttat
     1201 ggaatgagtg atatagctgg gcttatggta ctagaaagaa gaacaaatca gttcttaggt
     1261 ggacaaactc aaaaagattt ctcagatgct atggcaaaag atttagatga tcatgtaaaa
     1321 actactttaa atgaaagata tcaagttgta ttacaagctt taagagataa taaagatgca
     1381 attgaacaaa tgacttctga attacttgaa atagaggtaa ttacaggtga aagagttaga
     1441 gagattataa aagaacatgg tggaatagtt tttgaaggtg aagatttaca tagcgatagt
     1501 ttagataaaa gtgatttaaa agaagatatt aaatctgata aaactcaaat agaaaataaa
     1561 gatgataatc aatctgaaaa ataataattt ttagaagcaa ttttgcttct aaaaattttc
     1621 taaataataa aaattatcct tgacaaataa attaaattaa attatacttt caacctaatt
     1681 aaaaaaggag agtataaatg aaaatattta atatttcaaa aaacaatttt ctaaaaagtg
     1741 taaactctct actccttctt aaatattcac tctaaattat aaattttata gtttaagttt
     1801 cccaaaaaat ctgtttttct ttttttatta tc
//
LOCUS       DAOQAN010000246         1828 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_96992_length_1828_cov_2.187253, whole genome shotgun sequence.
ACCESSION   DAOQAN010000246 DAOQAN010000000
VERSION     DAOQAN010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96992_length_1828_cov_2.187253"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="PKO10_04495"
     CDS             complement(<1..199)
                     /locus_tag="PKO10_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HobA family DNA replication regulator"
                     /protein_id="HNW66440.1"
                     /translation="MHTNWTNNLQEFLNWTVDTIREDKLLSPWLEEKKFEWAPLVSKN
                     ITNILDKSYSILIITDKEREWF"
     gene            complement(177..1388)
                     /locus_tag="PKO10_04500"
     CDS             complement(177..1388)
                     /locus_tag="PKO10_04500"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNW66441.1"
                     /translation="MLKVLKFGGTSVGTLDRIANVAQIIKKIRDEGHDVIAIVSAMSG
                     ETNKLIEYAEYYTKTPDLKEMDMLLSSGERVTSALLSIALNSIGYKAISMTGREAGIM
                     TTDTHTKAKIEKIDTQKMKNALSEGNIIIVAGFQGVTLDGSRVTTLGRGGSDLSAVAI
                     AGAIKADVCEIYTDVDGIYTTDPRIEPKAKKLEKISYDEMLELASLGAKVLQNRSVEM
                     AKKLNVNLVSRSSFTPDVEGTLITNEEEIMEKPIVSGIALDKNQVRVGMYGVVDKPGV
                     ASAIFTALADANINVDMIVQTRGLDGTTDLDFTIPTTDLEICKKVMEQFRPQAKNIDY
                     NEAICKVSIVGVGMKSNTGVASKAFTAMANENINIRIISTSEIKISMIIDEKYAELAV
                     RALHDAYELDK"
     gene            complement(1409..>1828)
                     /locus_tag="PKO10_04505"
     CDS             complement(1409..>1828)
                     /locus_tag="PKO10_04505"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002789857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HNW66442.1"
                     /translation="SKYPAKCEIFIASRTDIPNAWQFPQGGIDEGENTKEALLRELEE
                     EIGTKDIEIIAEYPTWVSYLFPPVIAQKMYPYDGQKQKYYLVKLKKDAKININTEIPE
                     FSEYKFVSLKEVYEHITFFKRTVYKQVLKYFKDEGYI"
BASE COUNT          558 a          358 c          203 g          709 t
ORIGIN      
        1 aaaaccattc tctctctttg tctgtaataa tcaagataga gtaactttta tctaaaatat
       61 ttgtaatatt ttttgatact aatggtgccc attcaaactt tttttcttcc aaccaaggag
      121 ataaaagctt atcttccctg attgtatcaa ctgtccaatt taaaaactct tgcaaattat
      181 ttgtccaatt cgtatgcatc gtgtaaagct cttacagcta actctgcata tttttcatca
      241 ataatcatag atattttaat ctcacttgta gagataattc taatattaat attctcattt
      301 gccatagctg taaatgcttt tgaagcaact ccagtatttg atttcattcc aacaccaaca
      361 atagaaactt tacaaattgc ctcattataa tctatatttt ttgcttgagg tctaaattgt
      421 tccataactt ttttacaaat ctctaaatca gttgttggaa ttgtaaaatc taaatcagtt
      481 gttccatcaa gtcctcttgt ttgaacaatc atatcaacat ttatatttgc atctgcaaga
      541 gctgtaaaaa ttgcactagc aacacctggt ttatcaacaa caccatacat tccaactctt
      601 acttggtttt tatctaaagc tattccactt actattggtt tttccataat ttcctcttca
      661 tttgttatta aagttccctc tacatcgggt gtaaaactac ttcttgatac tagatttaca
      721 tttaattttt ttgccatttc aactgatcta ttttgtaata cttttgcacc aagacttgca
      781 agttccaaca tctcatcata agatattttt tctagttttt tagcttttgg ttcaattcta
      841 ggatctgttg tataaatacc atcaacatca gtataaattt cacaaacatc agcttttata
      901 gcaccagcaa ttgcaaccgc agataaatcg cttccacctc gacctaaagt cgtaactctt
      961 gaaccatcaa gtgtaactcc ttgaaatcca gcaactataa taatatttcc ctcacttaaa
     1021 gcatttttca tcttttgtgt atctattttt tctatttttg cttttgtgtg agtatctgtt
     1081 gtcataattc cagcttctct tcctgtcata gatatagctt tgtagccaat actatttaaa
     1141 gcaatagata ataaagctga agtcactctc tcacctgaac ttaaaagcat atccatctct
     1201 tttaaatcag gagtttttgt ataatattca gcatattcta ttaacttgtt tgtttcaccg
     1261 ctcatagcag aaacaatagc tataacatca tgaccctcat ctcttatctt tttaataatc
     1321 tgtgcaacat ttgcaattct atcaagtgtt ccaacacttg ttccaccaaa ttttaaaact
     1381 tttaacatct aatttactta tccttttttt aaatatatcc ctcatcttta aaatacttta
     1441 gaacttgctt gtagacagtt cttttaaaaa aagttatatg ttcataaact tcttttaaag
     1501 atacaaactt atattcacta aactctggaa tttcagtgtt tatgtttatt tttgcatctt
     1561 tttttaattt tactaaatag tatttttgtt tttgaccatc ataaggatac atcttttgag
     1621 caattacagg aggaaaaaga taagagaccc aagttggata ttcagctatt atttctatat
     1681 cttttgtacc aatctcttct tcaagctccc taagaagtgc ctcttttgta ttctcgcctt
     1741 catcaatccc accttgagga aactgccaag catttggaat atcagttctt gaagcaataa
     1801 aaatctcaca cttcgctgga tatttaga
//
LOCUS       DAOQAN010000247         1823 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_97456_length_1823_cov_2.719457, whole genome shotgun sequence.
ACCESSION   DAOQAN010000247 DAOQAN010000000
VERSION     DAOQAN010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97456_length_1823_cov_2.719457"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1643)
                     /locus_tag="PKO10_04510"
     CDS             complement(<1..1643)
                     /locus_tag="PKO10_04510"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HNW66443.1"
                     /translation="MFIRVKTTPNSPRKSVQIVESFRVDNKVKQKIVKHIGVALDDYE
                     LNELKLLASAIKTKLELENTLPLYTPEEIEKQIEKSKENIKRDIYSDDDYVVNIKDLV
                     EEDRVITGIHDIYGKMFDELDLKNIFVNPARNKSSVEYFRNIVLARIAKPDSKSRSVH
                     MLEENFGVSLDLDSVYKMMDKIDDKSIEKLNSIAYSETKKLFDDKIDVIYFDATTIYF
                     ESFTEDIGDDAIRKNGYSKDLKFNQPQVVLALMVTKEGLPIGYELFSGDTFDGHTLIP
                     SLKILRDKYNVDNVVYVADSGMFNNDNLKELEMLEEYNFNYIVGARIKNMQKNIKEQI
                     LNKNNYKELNSEISIANITLDNGRKLVVTYSLKRARKDKADREKAIKKLKDKLEKNIS
                     VKSQLSNQGYKKYLQLKSNNEESTCDLSIALNEDKIYEDSLWDGLKGLIVNKNSTLSN
                     DEILTQYNNLWQVEESFRITKHDLKIRPVYHWKPSRVKAHIAISFASLLIVRHLEYRI
                     KLQYKKLSPKVIRELLLNIQTSIVYDKTNIGSDKPLIESS"
BASE COUNT          580 a          288 c          174 g          781 t
ORIGIN      
        1 gatgattcaa ttaatggttt gtcagatcct atatttgttt tatcatacac tattgatgtt
       61 tgtatattta gcagtaactc tcttataact ttaggagata attttttata ttgaagtttt
      121 attctatact ctaagtgtct tacaattagt aaagatgcaa atgatattgc tatatgtgct
      181 ttaacacgtg atggtttcca atgatataca ggtcttattt tcaaatcatg ttttgtaatt
      241 ctaaagctct cttctacttg ccaaagatta ttatattgag ttaatatctc atcatttgaa
      301 agtgttgagt ttttatttac tattaaacct tttaaaccat cccaaagaga atcttcataa
      361 attttatctt catttaaagc tatagataaa tcacaagtag attcttcatt attacttttc
      421 aattgtaaat attttttata tccttgattt gagagttgtg atttaacaga gatatttttt
      481 tctaatttat cttttagttt ttttattgct ttttctctat ctgctttatc ttttcttgct
      541 cgttttaatg aatatgttac aactaatttt ctaccattat ctaaagtgat attagcaata
      601 ctaatttcac tatttaattc tttatagtta tttttattta aaatttgctc ttttatattt
      661 ttttgcatat tttttattct tgcgccaacg atataattaa agttatactc ttcaagcatc
      721 tcaagctctt ttaggttatc attattaaac ataccactat ctgcaacata tactacatta
      781 tcaacattat atttatctct tagtatttta aggcttggta ttagggtatg tccatcaaat
      841 gtatcaccac taaacagttc ataaccaata ggcaatccct cttttgtgac cattaatgct
      901 aatacaactt gtggttgatt aaattttaaa tctttactat atccattctt tctaatagca
      961 tcatcaccaa tatcttcagt aaaactttca aagtatatag ttgttgcatc aaagtatatt
     1021 acatctattt tatcatcaaa tagtttcttt gtttcactat atgcaattga atttaatttc
     1081 tcaatacttt tatcatctat tttatccatc attttataaa cgctatcaag atctaagcta
     1141 acaccaaagt tctcctctag catatgaaca ctacggcttt tactatcagg ctttgctatt
     1201 cttgctagca ctatatttct aaaatattca acactactct tatttctagc aggatttaca
     1261 aatatatttt ttaaatctaa ttcatcaaac atttttccat atatatcatg aatacctgtt
     1321 attactctat cttcctcaac taaatctttt atatttacta cataatcatc atcactataa
     1381 atatctcttt ttatattctc ttttgatttc tcaatttgtt tctcaatctc ttcaggggta
     1441 taaagtggaa gagtattctc taattctaat ttagttttaa tagctgatgc taataatttt
     1501 aattcattta attcataatc atctaaagca acaccaatat gttttacaat cttctgtttt
     1561 actttattat caactctaaa actctcaaca atttgaacag attttctagg actatttggt
     1621 gtagttttaa ctctaataaa catttaatta cctcaattaa tataaatgta attattatat
     1681 aaaaaacctt aaataatctt tatatacata ataactaggc actacaaaat taatttaaaa
     1741 atagtttgcc ttatttacgg aactgaaaaa cagtattaaa tattattatg cggaagtcgg
     1801 gagttttatc tcttcttcat tta
//
LOCUS       DAOQAN010000248         1822 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_97535_length_1822_cov_2.737408, whole genome shotgun sequence.
ACCESSION   DAOQAN010000248 DAOQAN010000000
VERSION     DAOQAN010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97535_length_1822_cov_2.737408"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /gene="murB"
                     /locus_tag="PKO10_04515"
     CDS             complement(<1..482)
                     /gene="murB"
                     /locus_tag="PKO10_04515"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014475400.1"
                     /note="catalyzes the reduction of UDP-N-acetylglucosamine
                     enolpyruvate to form UDP-N-acetylmuramate in peptidoglycan
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HNW66444.1"
                     /translation="MSEKIANYYKTVDFKRYSSIHIGPIKEVLVINEIGDYSDFQIIG
                     RGNNLLISPKCEKKFAILGEEFDYIKDEDDLLYVGCATSSGKLLTYTRKNDIASLEFL
                     AKLPGNLGGLVKMNAGLKSWEIFNYIHSIKTKDGYIKKENVDFSYRETKIDTIVYEVV
                     "
     gene            complement(479..1345)
                     /locus_tag="PKO10_04520"
     CDS             complement(479..1345)
                     /locus_tag="PKO10_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014475401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase"
                     /protein_id="HNW66445.1"
                     /translation="MRTISVGHSPDADDIFMYYAIKFGWVGIENTKFENIALDIETLN
                     QATLKGIYDICAISFALYPFVKDDFALLKTAVSFGEGYGPKLIKKKGTKLKKNFKVAL
                     SGEFTTNALLFKIAYPDARISYMNFLDIENAVINGDVDAGVLIHESILTYSSVLEVER
                     EIWDIWVELCNGEELPLPLGGMCLRRSIPLHEAIKYENALIKAVDVANKNRKTLAPML
                     LEKGLIRVDATTLDKYLDLYANDNSVKMSQIQYKALNKLFELGYKSGHYQNLIKAEDF
                     LIPSEYEELRAR"
     gene            complement(1342..>1822)
                     /locus_tag="PKO10_04525"
     CDS             complement(1342..>1822)
                     /locus_tag="PKO10_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66446.1"
                     /translation="SAGEEEFEEAFVKFEEASKRALFVGDDLINHERVENIVKLLANI
                     KKYTDFELIFSDKTFEEKVNSCSDLSLDEIDDLQTFNGTLVYFTNIENNEKLIASQTF
                     LNIAKLKSGDMASFKIDDKIYKKEVVLDKNLLGTIALISNPTSNYRFAKIVLNKEQN"
BASE COUNT          613 a          323 c          188 g          698 t
ORIGIN      
        1 aaaactactt cataaactat agtatcaatt tttgtctctc tatatgaaaa atctacattc
       61 tcttttttga tatatccatc tttagttttt attgagtgaa tatagttaaa aatctcccaa
      121 gattttaaac ctgcattcat ctttacaaga ccacctaaat taccaggtaa ttttgctaaa
      181 aactccaaag aggcaatatc attttttctt gtatatgtaa gaagtttacc actacttgta
      241 gcacaaccta catatagcaa atcatcttca tcttttatat aatcaaactc ttcacccaaa
      301 attgcaaact ttttttcaca ttttggagag ataagtaaat tatttcctct tccaattatc
      361 tgaaaatctg aataatctcc tatttcgttt ataaccaaaa cctcttttat aggaccaata
      421 tgtatagaag agtatctttt aaaatctact gttttatagt aattagctat tttttcactc
      481 atctagctct taactcttca tattcacttg ggattaaaaa atcttctgct tttattaaat
      541 tttggtagtg tccactttta taacctagtt caaaaagttt atttagagct ttatattgaa
      601 tttggctcat ttttacagag ttatcatttg catatagatc taaatattta tctaaagttg
      661 tagcatctac acgaattaag cctttttcta aaagcatagg agctagagtt tttctatttt
      721 tatttgctac atcaacagct tttataagtg cattttcata ttttatagct tcgtgaagcg
      781 gaattgaacg tcttaaacac attccaccaa gaggaagtgg caactcttca ccattgcata
      841 gttctaccca aatatcccaa atctctcttt caacttcaag aacactgcta taagttaaaa
      901 ttgattcatg aattaaaact cctgcatcta catcaccatt tattacagca ttttcaatat
      961 ctaaaaagtt catatacgaa attctagcat ctggataagc tattttaaat aaaagagcat
     1021 ttgtagtaaa ctctccacta agagctactt taaaattttt ctttagttta gttccttttt
     1081 tcttaatcaa ctttggtccg tacccttctc caaatgaaac agcagtttta agtaaggcaa
     1141 aatcatcttt aacaaaagga tacaaagcaa aagagatagc acaaatatcg taaatccctt
     1201 ttagtgttgc ttgatttaaa gtctctatat caagtgcaat attttcaaat tttgtattct
     1261 ctataccaac ccagccaaac ttgattgcat aatacataaa tatatcatct gcatcaggag
     1321 agtgtcctac gctaatagtt ctcaattttg ctccttattt aaaacaattt ttgcaaatct
     1381 ataatttgat gttggatttg atattaaagc aatagttcct aaaaggtttt tatctaaaac
     1441 tacctctttt ttatatattt tatcatctat tttaaaacta gccatatcgc cactttttaa
     1501 ttttgcaata tttaaaaaag tttgacttgc aattaacttt tcattatttt caatatttgt
     1561 aaaataaact aaagttccat taaatgtttg taaatcatca atttcatcta aagataaatc
     1621 agaacaagaa tttacttttt cttcaaaagt tttatcacta aatataagtt caaaatctgt
     1681 atatttttta atatttgcta gaagttttac aatattttca actctttcgt gatttattaa
     1741 atcatcacca acaaaaagag ctctttttga agcttcttca aattttacaa aagcctcttc
     1801 aaactcctct tctcctgcac ta
//
LOCUS       DAOQAN010000249         1816 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_98027_length_1816_cov_3.007382, whole genome shotgun sequence.
ACCESSION   DAOQAN010000249 DAOQAN010000000
VERSION     DAOQAN010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98027_length_1816_cov_3.007382"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..761
                     /locus_tag="PKO10_04530"
     CDS             <1..761
                     /locus_tag="PKO10_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014473104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HNW66447.1"
                     /translation="DYHASGILASKLLPKNQVVTFFHNDEKDLEDKLKDCASKGRKII
                     AIEGVYSMSGNIAKKEIFDLSKKYNTLLIVDEAHSSGVIGENLLGIFDYYNIKIENNH
                     IKMGTLGKAYGSYGAYILSSKSIIEFLQNRAKPIIYSTAPSLFDTALGYESLKYILRN
                     KEKLKQKIKDNLSIIQGYLGISSKSLIIPILVNDNKKVLKIQKILKENGFLVGAIRQP
                     TVKNAIIRLIAKIDINSDELKKACNLIKDLNANK"
     gene            748..1401
                     /locus_tag="PKO10_04535"
     CDS             748..1401
                     /locus_tag="PKO10_04535"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013134088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HNW66448.1"
                     /translation="MQINDLIKGNKKFREVRFSKYETDLKQLSKEGQNPDILFIGCSD
                     SRVTPELVLDTRPGDMFTLRNVGNFVPPYNPDEDFHGTSAVIEYAVNVLEVKHIIVCG
                     HSHCGACKSLYQDLGDGPDLINVKKWLELGKKAKEYTLLATLDKNDKEQLYRTTEKVS
                     IVYQMENLLTFPYIVRRIKEGTLQIHGWYYKIENGSIEFYDGSDCTFKPLEEFKNEL"
     gene            1391..1708
                     /locus_tag="PKO10_04540"
     CDS             1391..1708
                     /locus_tag="PKO10_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66449.1"
                     /translation="MSYETRKLPKKTIVIISIIIALGIFIFIWLEVLKNQKFNEVLAD
                     LGHKDIKNLKVVNRINVEDTITKEKSYVYKLTFFDNTLNKTCIGFVSKQKDKTYTKDF
                     DCK"
BASE COUNT          743 a          175 c          299 g          599 t
ORIGIN      
        1 aagattatca tgcaagtgga atattagctt ctaaacttct tcctaaaaat caagttgtaa
       61 ctttttttca taatgatgaa aaagatttag aagataaatt aaaagattgt gcatcaaaag
      121 gacgaaaaat tatagctatt gaaggtgttt actcaatgag cggaaatatt gcaaaaaaag
      181 agatttttga tttatcaaaa aaatataata cattattgat tgttgatgaa gctcatagtt
      241 caggtgttat tggtgaaaat cttttgggaa tttttgatta ttacaatatt aaaatagaaa
      301 ataaccacat aaaaatgggt actttaggta aagcttatgg ttcgtatgga gcatatattt
      361 tatcttcaaa aagcataata gaatttttac aaaatagggc aaaaccaatt atttattcaa
      421 cagctccctc tctttttgat acagctttag gatatgaaag tttaaaatat attttaagaa
      481 ataaagaaaa actaaagcaa aaaattaagg ataacttaag tataatccaa ggctatttag
      541 gaataagctc aaaatcgctt ataattccaa tactagtaaa tgacaataaa aaagttttaa
      601 agatacaaaa aatcttaaaa gaaaatggtt ttttggttgg tgcaataagg caaccaacag
      661 ttaaaaatgc gattattcga ctcattgcga agattgatat aaattctgat gagctgaaaa
      721 aagcttgcaa tttaataaag gatttaaatg caaataaatg atttaatcaa aggtaacaaa
      781 aaatttagag aagttagatt ttcaaaatat gaaacagatt taaaacaatt atcaaaagag
      841 ggacaaaatc cagatatttt gtttattggt tgtagcgata gtagagttac tcctgagttg
      901 gttttagata caagacctgg agatatgttt actcttagaa atgttggaaa ttttgttcca
      961 ccatataatc ctgatgaaga ttttcacgga acaagtgctg ttattgaata tgctgtaaat
     1021 gttttagaag ttaagcatat tattgtttgt ggacactctc attgtggagc ttgtaagagt
     1081 ttgtatcaag atttaggaga tggtcctgat ttgataaatg ttaaaaaatg gctagaatta
     1141 ggaaaaaaag caaaagagta tacgctttta gcaacacttg ataaaaatga taaagaacaa
     1201 ctatatagaa caactgaaaa agtatctatt gtttatcaaa tggaaaacct tttgactttc
     1261 ccatatattg ttagaagaat aaaagaggga acgcttcaaa ttcatggttg gtactataaa
     1321 atagaaaatg gaagtataga attttatgat ggaagtgatt gtacatttaa accactagaa
     1381 gagtttaaaa atgagttatg aaacaagaaa attacctaaa aaaactattg ttataatatc
     1441 tattattatt gctttaggaa tttttatatt tatttggttg gaagttttaa aaaatcaaaa
     1501 atttaatgaa gttttagctg acttaggaca taaagatata aaaaatttaa aagtagtaaa
     1561 taggattaat gttgaagata ctataactaa agagaaaagt tatgtttata aattaacatt
     1621 ttttgataac actttaaata aaacttgtat aggttttgta tctaaacaaa aagataaaac
     1681 atatacaaaa gatttcgatt gcaaataaga ggataatatg actttgattg ataaattaga
     1741 aaatggtact aggttaaact atgaagatgg agttaatctt tttgatttag atgttttaac
     1801 tttaggagat tatgca
//
LOCUS       DAOQAN010000250         1815 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_98128_length_1815_cov_2.642614, whole genome shotgun sequence.
ACCESSION   DAOQAN010000250 DAOQAN010000000
VERSION     DAOQAN010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98128_length_1815_cov_2.642614"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="PKO10_04545"
     CDS             <1..202
                     /locus_tag="PKO10_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014474486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HNW66450.1"
                     /translation="PIATILSAAMMLKYTLNEQKASDMIEKAIKDALKDGYRTKDLAA
                     FDAKEVLNCVAMGNKIVEYINK"
     gene            212..1141
                     /locus_tag="PKO10_04550"
     CDS             212..1141
                     /locus_tag="PKO10_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HNW66451.1"
                     /translation="MSEKKYRLVTRSDMDGLVCGTLLKYLDIIDEITFVHPKDMQDGL
                     IEITNNDITTNLPYVDGVYLAFDHHFSETLRNEKRDNHIINPNAPSAAQVVYDYYNGD
                     EVFPGYFTSMMQGANKADSANFLYEDIVRPRAWTLLSFLMDSRTGLGRFRNFRISNYQ
                     LMMDLIDYCARHNIDEILELPDVKERVDLYFQYEEEFKEQLRKCTKVYNNLVIIDYRD
                     EEIIYPGNRFMVYTLFPEQNISIHIFYAKDKDKVVFSTGKSITNKTSKTNIGELMLQY
                     GGGGHENAGACQIYKEESEKVLKELIEKINQDG"
     gene            complement(1160..1519)
                     /locus_tag="PKO10_04555"
     CDS             complement(1160..1519)
                     /locus_tag="PKO10_04555"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HNW66452.1"
                     /translation="MQLFKNDLINIEIENSEIPWLKIFTNKNIKEFSQCDNKTKQEIW
                     KYLDIIEKKMIEYYNPDKINIASFGNYVPHVHFHIMARFKEDSFFPEPMWGKKQREAN
                     LDLPSFEIFIDELKKYF"
     gene            1738..>1815
                     /gene="rpmJ"
                     /locus_tag="PKO10_04560"
     CDS             1738..>1815
                     /gene="rpmJ"
                     /locus_tag="PKO10_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HNW66453.1"
                     /translation="MKVRASVKKMCDKCKVIKRRGIVRVI"
BASE COUNT          701 a          195 c          289 g          630 t
ORIGIN      
        1 tccaatagca acaattttaa gtgctgctat gatgttaaaa tatacattaa atgagcaaaa
       61 agctagtgat atgatagaaa aagcaataaa agatgcttta aaagatggat atagaacaaa
      121 agatttagca gcttttgatg caaaagaggt tttaaattgt gttgctatgg gtaataaaat
      181 agttgagtat ataaataaat aaaggttatt aatgagtgaa aaaaagtaca gacttgtaac
      241 acgaagtgat atggatggac ttgtttgtgg tacattgctt aaatatcttg atattataga
      301 tgaaattact tttgttcacc caaaagatat gcaagatggg ttgattgaga ttacaaataa
      361 tgatataaca actaatcttc cttatgtaga tggagtttat ttagcatttg atcatcactt
      421 tagtgaaaca ttaagaaatg aaaaaagaga taatcatatt ataaatccta atgctcctag
      481 tgctgcacag gttgtttatg attattataa tggagatgaa gttttccctg gatattttac
      541 atctatgatg caaggtgcaa ataaagctga tagcgcaaat tttttatatg aagatattgt
      601 aagaccaaga gcttggacac tattaagctt tttaatggat tcaagaactg gacttggaag
      661 atttagaaat tttagaatat caaactatca attaatgatg gatttaatag actattgtgc
      721 tagacataat attgatgaaa ttttagaact acctgatgta aaagagagag ttgatttata
      781 ttttcaatat gaagaggaat ttaaagagca acttagaaaa tgtacaaaag tttataataa
      841 cttggtaata atagattata gagatgaaga gattatttat cctggaaata gatttatggt
      901 ttatacactt tttcctgaac aaaatatatc tattcatatc ttctatgcaa aagataaaga
      961 taaagtagta tttagtactg gaaaatctat tacaaataaa acttctaaaa caaatattgg
     1021 tgaactaatg cttcaatatg gtggtggagg gcatgaaaat gctggtgctt gtcaaattta
     1081 taaagaagaa tcagaaaaag ttctaaaaga gttaatagaa aaaatcaacc aagatggttg
     1141 attgatattt aaaaaagagt taaaaatatt tttttaactc atctataaaa atttcaaaag
     1201 agggtaaatc taagtttgcc tctctttgct ttttgcccca cattggttct gggaaaaagc
     1261 tatcttcttt aaatcttgcc attatatgaa aatgtacatg aggaacatag tttccaaaag
     1321 atgcaatgtt tatcttatct ggattataat actcaatcat ttttttctct ataatatcaa
     1381 gatatttcca aatttcttgt tttgttttgt tatcacattg tgaaaactct ttaatatttt
     1441 tatttgtaaa aatctttaac caaggaattt cactattttc tatctctata tttattaaat
     1501 cgtttttaaa tagttgcata ttttactccc aatatttata ttcttgaaaa ttatagcata
     1561 ttaaactatc cttaagcaat aattaaatac aatgcagttc cattttttca accatgaaag
     1621 aagtttttat aaaattaatc aattaaattt gtattttata gagagttgga agagttttat
     1681 tttagatttt tcctgttgaa aattggaaaa aaacacaaac aaagaaggac gagacttatg
     1741 aaagtaagag cttcagtaaa aaaaatgtgt gataaatgta aagttatcaa aagaagaggt
     1801 atcgtaagag taatc
//
LOCUS       DAOQAN010000251         1809 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_98636_length_1809_cov_2.173888, whole genome shotgun sequence.
ACCESSION   DAOQAN010000251 DAOQAN010000000
VERSION     DAOQAN010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98636_length_1809_cov_2.173888"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1002
                     /locus_tag="PKO10_04565"
     CDS             <1..1002
                     /locus_tag="PKO10_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HNW66454.1"
                     /translation="EDEEIQKNMEFTKTAITKATVESQPIKAQVYDYKEEILKMQSLL
                     EQVQKTLWKPKSQLYDLLIPPEFIDVYTIFEKNEFDSEMTYTIMKKTIKELPVSLKSN
                     QKKVNDFFKLVLRRVIPIKMEQPLRKEQRKIMMMVGPTGVGKTTTISKLAARFAYKME
                     QNHKVGVITLDSFRVGAIEQLQAYTNIMRLPLEIVKKPEDLTEALLRLKNCNYIFIDT
                     AGSSQYDIDKIELINEYRNHVYELPIEKTLVLPSNVKHSDLMEIYKNYSILDIDNLIF
                     TKLDETKSFGNVISFSHKTKKSITYFSIGQNVPDDLIAADASFLIDCFMNNECSKEI"
     gene            1002..>1809
                     /locus_tag="PKO10_04570"
     CDS             1002..>1809
                     /locus_tag="PKO10_04570"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HNW66455.1"
                     /translation="MLDARLSQAEKLINLTSKVNSEVSSSKTKLLTITSGKGGVGKST
                     FTANFAYILSQKNLRVLVLDADIGLANMQVLFDVKPVVTLFDYINGHKKLQEVIIETK
                     YPNLSLIAGKSGYQYATNSSSFIFSRLVQDILDLNFYDILIVDTGAGLNDYVKEFLKV
                     STNILAITSTDPSALTDVYSLIKMLAIDKKSLMLCFNHTKNDLVGETISNSLINLAKK
                     NRLKSEFVIKYIGSVPSSENITKIARARRIFVNEFPQEEASFKLHSIVDRV"
BASE COUNT          727 a          218 c          286 g          578 t
ORIGIN      
        1 gaagatgaag agattcaaaa aaatatggag tttacaaaaa cggcaattac taaagctact
       61 gttgaatctc aacctataaa agcacaagtt tatgactata aagaggagat tttaaaaatg
      121 caatctcttc ttgaacaagt acaaaaaact ctttggaaac caaaaagcca actatacgat
      181 ttgcttatac ctccagaatt tatagatgtt tacactatct ttgaaaaaaa tgagttcgat
      241 agtgagatga catacactat tatgaaaaag acaataaaag aacttccagt ttcactaaaa
      301 tcaaatcaga aaaaagtaaa tgatttcttt aaattagttt taagaagagt tattcctatt
      361 aaaatggagc aacctttaag aaaagagcaa agaaaaatta tgatgatggt aggtcctaca
      421 ggagttggaa aaactacaac tatttcaaaa ctagctgcaa gatttgctta caaaatggaa
      481 caaaaccata aggttggagt aattacttta gactctttta gagtaggtgc aatagaacag
      541 ctacaagcat atacaaatat tatgaggctt cctttagaaa tagtaaagaa gcctgaagat
      601 ttgactgaag cacttttaag acttaaaaat tgtaattata tttttattga cacagcaggt
      661 tcaagtcaat atgatataga taaaatagaa cttataaatg agtacagaaa tcatgtatat
      721 gaacttccta ttgagaaaac tttggtttta ccttcaaatg taaaacatag tgatttaatg
      781 gagatttata aaaactattc aatacttgat attgataatt taatctttac aaaattagat
      841 gagacaaaaa gttttggaaa tgttatatcc ttttcacata aaacaaaaaa atcaataaca
      901 tatttctcta ttggacaaaa tgttcctgat gatttaattg cagctgatgc ttcattttta
      961 atagattgtt ttatgaataa tgagtgcagt aaggagatat aatgctagat gctagactat
     1021 ctcaagccga aaaattaata aatcttactt caaaagtaaa tagtgaagta agtagctcaa
     1081 aaacaaaact tttgacaata acatctggaa aaggaggagt tggaaaatct acttttacag
     1141 ctaattttgc ttatatatta tctcaaaaaa atttaagagt attggtttta gatgcagata
     1201 taggtttggc aaatatgcag gtactttttg atgtaaaacc agtagttact ctttttgact
     1261 atattaatgg gcataaaaaa cttcaagaag taataataga aacgaaatac ccaaatttat
     1321 ctttaattgc tgggaaaagt ggataccaat atgcaacaaa tagtagtagt tttatatttt
     1381 ctagattagt tcaagatata ttggatttaa atttttatga tattttgatt gttgatactg
     1441 gagctggatt aaatgattat gtaaaagagt ttttaaaagt atcaacaaat attttagcaa
     1501 taacttcaac agatcctagt gctttaactg atgtctactc tttgataaaa atgttagcaa
     1561 tcgataaaaa gagtttaatg ctctgtttta atcatacaaa aaatgatcta gttggtgaaa
     1621 caatttcaaa ctctttgata aatttagcta aaaaaaacag attaaaaagc gaatttgtga
     1681 taaaatatat aggaagtgta ccaagcagtg agaacattac aaaaatagct agagctagaa
     1741 gaatatttgt taatgagttt ccacaagagg aagcttcttt taagttgcat agtattgtag
     1801 atcgtgttt
//
LOCUS       DAOQAN010000252         1793 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_100033_length_1793_cov_3.670886, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000252 DAOQAN010000000
VERSION     DAOQAN010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100033_length_1793_cov_3.670886"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="PKO10_04575"
     CDS             complement(<1..605)
                     /locus_tag="PKO10_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HNW66456.1"
                     /translation="MLKAKSLYHLIVYSIFFIIILISFFTFIIINNAHEELQEKITTL
                     KEDYTNNQKALLKNYVNYVVKFIDYYYIENRDKFDEETIQKNLILALDHLNISSNPNE
                     YIFIFDINGNLIDNSNDKQEIGKNFLNYKDSKGKSVVKELIEASKNSDGGFVNYFWSK
                     PSLTKETNKISYVKSYDRWSWTIGKGVYLDENVKNIEVLYRS"
     gene            complement(674..750)
                     /locus_tag="PKO10_04580"
     tRNA            complement(674..750)
                     /locus_tag="PKO10_04580"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(714..716),aa:Asp,seq:gtc)
     gene            complement(764..839)
                     /locus_tag="PKO10_04585"
     tRNA            complement(764..839)
                     /locus_tag="PKO10_04585"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(804..806),aa:Val,seq:tac)
     gene            complement(859..933)
                     /locus_tag="PKO10_04590"
     tRNA            complement(859..933)
                     /locus_tag="PKO10_04590"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(898..900),aa:Glu,seq:ttc)
     gene            complement(954..1029)
                     /locus_tag="PKO10_04595"
     tRNA            complement(954..1029)
                     /locus_tag="PKO10_04595"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(994..996),aa:Lys,seq:ttt)
     gene            complement(1084..1587)
                     /locus_tag="PKO10_04600"
     CDS             complement(1084..1587)
                     /locus_tag="PKO10_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014473361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HNW66457.1"
                     /translation="MATNFSKKDMIKLQNLLRKSKKTITTAESCTGGLVASLITKISG
                     SSDIFNGSIVTYSNSIKNKELGVLNESLDKFGAVSKQVVSEMLDGVLKKFEADFAIAI
                     SGIAGPNGGTKNKPVGTIVIGIASNNNIKKIKTYHLKGSRISIQKQSANIALSKILKF
                     VSKTIDN"
     gene            complement(1626..>1793)
                     /locus_tag="PKO10_04605"
     CDS             complement(1626..>1793)
                     /locus_tag="PKO10_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HNW66458.1"
                     /translation="EASITEEKGIIYNMTPSPGATSCLGNAERDIKIVCEYLGKKFNE
                     EQFLADFTDDK"
BASE COUNT          586 a          289 c          242 g          676 t
ORIGIN      
        1 gatctgtata gtacctctat atttttgaca ttttcatcta aatatacacc ttttcctatt
       61 gtccaactcc atctatcata agatttaaca taagatattt tatttgtctc tttagttagg
      121 ctaggttttg accaaaaata gtttacaaat ccaccatcac tattttttga agcttctatt
      181 agctctttta caacactctt tcctttagag tctttataat ttaggaaatt tttgccaatt
      241 tcttgtttat cattagaatt atcaattaaa tttccattaa tatcaaaaat aaaaatatac
      301 tcatttggat ttgagctaat atttagatga tctaaagcta aaataagatt tttttgaata
      361 gtttcttcat caaatttatc tctattttca atatagtagt aatcaataaa tttaactaca
      421 taatttacat aatttttaag tagagctttt tgattgtttg tataatcttc ttttaaagta
      481 gttatctttt cttgaagttc ttcatgtgca ttgtttataa taataaaagt aaaaaaagat
      541 attaaaatga taataaaaaa tatactgtaa acaattaaat gatatagact tttagctttt
      601 agcaattttc aaccttaaaa tataaaaatt aataatagct agatttgaat taagagatag
      661 ttaaaaaata aaatggcgcg gccaacgaga ctcgaactcg cgacctcctg cgtgacaggc
      721 aggcattcta accaactaaa ctatggccgc accttttaaa taatggtggt cgatataaga
      781 ctcgaactta tgacatctac cttgtaaggg tagcgctcta ccaactgagc taatcgacca
      841 tttgttgcat attgtacttg gtgaccccta ggggattcga acccctgtgg catggatgaa
      901 aaccatgaat cctaaccgtt agatgaaggg gccaagatta tgcaacaaca aaatggtgac
      961 ccgtgatgga ttcgaaccat cggcctcctc attaaaagtg agatgctcta ccggctgagc
     1021 taacgagtca ttcaaatttt aagtgtttac ttaaaatgga ctggaattat agtgcaattt
     1081 aaattaattg tcaatagttt ttgatacaaa tttcaaaatt tttgaaagag ctatatttgc
     1141 tgattgtttt tgaattgaaa ttctgctacc ttttaggtga tatgttttta ttttttttat
     1201 attattattt gaagcaatac ctattacaat agttccaaca ggcttatttt ttgttccacc
     1261 atttggacca gcaattcctg aaattgcaat agcaaaatct gcttcaaatt tttttaatac
     1321 accatctagc atctcagata caacttgttt acttactgcc ccaaatttat ctaagctttc
     1381 atttaaaaca cctaactctt tgttttttat actatttgag taagtaacaa tacttccatt
     1441 aaatatatca ctagagcctg agattttagt aataagactt gcaactagtc cacctgtaca
     1501 agattcagca gttgtaattg ttttttttga ttttcttaaa agattttgaa gtttaatcat
     1561 atcttttttt gagaagtttg ttgccataaa aagccttttg ttaaaaaggg caatagccct
     1621 ttttattatt tatcatctgt aaaatcagct aagaattgtt cttcgttaaa ttttttacct
     1681 aagtattcac aaactatttt tatatctctt tcagcatttc ctaaacaaga agttgctcct
     1741 ggagatggag tcatattata aataatccct ttttcttcag taattgaagc ttc
//
LOCUS       DAOQAN010000253         1792 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_100111_length_1792_cov_4.172712, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000253 DAOQAN010000000
VERSION     DAOQAN010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100111_length_1792_cov_4.172712"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(143..1510)
                     /locus_tag="PKO10_04610"
     CDS             complement(143..1510)
                     /locus_tag="PKO10_04610"
                     /inference="COORDINATES: protein motif:HMM:NF033578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HNW66459.1"
                     /translation="MLSNSSKTKQQNLFYSPLSDMLDMNDPLIALSNAIDWEIFENEF
                     SQFYSKDGSPAKPIRLMVGLLILKQLENLSDESIVIQWKRNPYYQYFCGYNEMQLCEP
                     CHSTQLVKFRQRIGKEGMELIFKVSVQLHGNKAEEKEVLIDTTVQEKNITYPTDGKLA
                     IKMIHHLHKIGKKEQLNLRRTFVKEIKEHRINLRFFKHPKKIKKARASIKRLRTIVGI
                     LIRDISRILQPEQLVNYQEKFDLFDKVRNQQIKDSNKVLSLHETHVYAITKGKDHKKY
                     EYGTKASIVATKDSGVIVGVASHSKNEHDSKTLEAALISANKTRTTAIKEVICDRGYR
                     GIKEVLGTIISIPGTTLKKDTEYQKQQKRDKFKRRAAIEPIIGHLKSDFRLARNYLKG
                     FIGDQINLLLAATAWNLKKWMNIYFFAIISGEMKLLLESIKQIKLYNQIFLQLIVIKL
                     IFTHP"
BASE COUNT          547 a          271 c          220 g          754 t
ORIGIN      
        1 ttaaaacaag attatgagtt gagactttcg tcttatgcct ttgattcttc atcaccaatg
       61 attattacag atgaaaaaaa caatataatt agtcacccct gaaaaagcct aaattatttt
      121 atttcttact aactttttat tctcatggat gagtaaatat tagttttatc acaatcaatt
      181 gtaaaaatat ttggttgtac aatttgattt gctttattga ttctagtagt aatttcattt
      241 caccagaaat aattgcaaaa aagtagatat tcatccactt ttttaggttc catgcagttg
      301 cggctagaag taaatttatt tgatcaccaa taaaaccttt aagataattt cttgctaatc
      361 taaaatctga ttttaaatga ccaataatag gttctatcgc agctcttcgt ttaaacttat
      421 ctcttttttg ttgcttttga tattctgtat cttttttaag cgtagttcct ggtattgata
      481 ttattgttcc aagtacttct tttattcctc tatatcctct atcacaaata acttctttaa
      541 ttgctgtagt tcttgtttta ttagctgata ttaaagctgc ttctagagtt tttgagtcat
      601 gttcattttt actatgagat gctacgccta caattactcc tgaatctttt gttgcaacaa
      661 ttgatgcttt tgttccatat tcatactttt tatgatcttt accttttgtg attgcataca
      721 catgtgtttc atgaagagat aaaactttat tactatcttt gatttgttgg tttcttactt
      781 tatcaaatag atcaaatttc tcttgatagt ttacaagttg ttctggttgt agtattctgc
      841 tgatatctct tatgagtatt cccacaatag ttcttaatcg tttaatagat gctctagctt
      901 ttttaatttt ctttgggtgt ttaaaaaatc ttaaattaat tctatgttct tttatttctt
      961 ttacaaaagt tcttcgtaaa tttaattgct cttttttacc aatcttatga agatgatgaa
     1021 tcatttttat tgctaatttc ccatctgttg ggtaagtgat atttttctct tgtactgttg
     1081 tatcaataag aacttctttt tcttcagctt tatttccatg aagttgtaca ctaactttaa
     1141 aaattagctc cataccttct ttaccaattc tttgtctaaa tttaacaagt tgtgtgctat
     1201 gacatggttc acaaagttgc atttcattat atccacagaa atactgataa tatggatttc
     1261 gtttccattg aattacaata ctttcatctg atagattctc taattgttta agtatcaata
     1321 atcctaccat taatcttatt ggtttagcag gagatccatc tttagaataa aattgagaaa
     1381 actcattttc aaatatttcc caatcgattg catttgataa tgcaatcaaa ggatcattca
     1441 tatcaagcat atctgaaaga ggactataaa ataaattttg ttgttttgtt ttagaagaat
     1501 tagaaagcat ttttctcatc catttcgacc aaaaacataa agtttttgtt aatatttata
     1561 tagatatttt atctaaaaaa tgcttataaa gtgcctaaat acagtacttt atcgagtttt
     1621 tcagggatga ctaattagcc ttgattgctt ttctaaagcg acactttaca acaatatcgc
     1681 ttagctaatg aaacttaatt aaactaaacc tctttataga tattatttaa atcattattt
     1741 atttgattaa ttacttttgg tctaaacttt aaaaaatcat ctaaatagtt gt
//
LOCUS       DAOQAN010000254         1792 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_100147_length_1792_cov_2.612550, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000254 DAOQAN010000000
VERSION     DAOQAN010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100147_length_1792_cov_2.612550"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="PKO10_04615"
     CDS             complement(<1..322)
                     /locus_tag="PKO10_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HNW66460.1"
                     /translation="MYKYLIIFIFATFLNAQDFKIASYNVENFFDLSYDKTEYDEYIP
                     NNKSLWNQRNFNIKLENIIKVIEDLDADIIALQEIENENLIKLLKQKLPQYSYYNFTK
                     YPSSA"
     gene            503..910
                     /locus_tag="PKO10_04620"
     CDS             503..910
                     /locus_tag="PKO10_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HNW66461.1"
                     /translation="MLNISGLLKDYDLKVTPQRVAIVEELYTNGHMNIDEIYKKLIQR
                     FPSVSLATIYKNINSMVERVFLSEVKIPNEKSVYELIKTEHAHLVCKDCGFIEDIMLD
                     SSDIVEQVSKLTPFKVDSTNIVLSGVCSKCTNC"
     gene            complement(912..1331)
                     /gene="ybeY"
                     /locus_tag="PKO10_04625"
     CDS             complement(912..1331)
                     /gene="ybeY"
                     /locus_tag="PKO10_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HNW66462.1"
                     /translation="MIDFENQTDFLVNLVALEKIVTSLTKKDLELIIVDNQTIKEINL
                     EHRKKDEATDVLSFPVDGDFEHLPLGTIIISIDFVKEKAKEYGHSEEDELNLLFIHGL
                     LHLLGYDHEVDNGEHRQKEEELIKQFNLPSSLIVRNS"
     gene            1397..>1792
                     /locus_tag="PKO10_04630"
     CDS             1397..>1792
                     /locus_tag="PKO10_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66463.1"
                     /translation="MSLTVGKKASIDYKVEKKDLASNLNISADLTFPEVFATARMIAL
                     MECSAAKLMLPLLKDDEKSVGVNVNITHMAATLENDVAISTATFVGMEGKLYKFEIEV
                     IDSAGVCGRGTHTRAIVSTSRLIEGAQRRA"
BASE COUNT          638 a          206 c          258 g          690 t
ORIGIN      
        1 ctgcacttga tggatatttt gtaaagttat aatatgaata ttgtggtaat ttttgtttta
       61 atagttttat taaattttca ttttctatct cttgtagagc aataatatca gcatctaaat
      121 cttctattac ttttattata ttttctaatt ttatgttgaa atttctttga ttccataatg
      181 atttattatt tggaatatac tcatcgtatt ctgttttatc gtagcttaaa tcaaagaaat
      241 tttctacatt ataagatgct attttaaaat cttgagcatt taaaaaagta gcaaaaataa
      301 agattattag atatttatac aagttttatt tccaaatttt ttggaaattg tagcaaatat
      361 tttattttta aatggtaaat gtaaaatatt tattactcta ttttttaata aaatttatta
      421 tttgataatt tccaagaata atttgatttt ttttttgata taatcatcaa cattgtactg
      481 tatatataaa aaaggaaaat ttatgctgaa tatttcagga ttattaaaag actatgactt
      541 aaaagttact ccacaaagag ttgctattgt tgaagagtta tatacgaatg ggcatatgaa
      601 tattgatgaa atttataaaa aattgataca aagatttcca tcagtttctc ttgcaactat
      661 ttataaaaat ataaactcta tggttgagag agtttttcta agtgaagtta aaattccaaa
      721 tgaaaaatct gtttatgagt taataaaaac tgagcatgca cacttagttt gtaaagattg
      781 tggatttatt gaagatatta tgcttgattc aagtgatata gtggagcaag tttctaaact
      841 tactcctttt aaagtagatt caacaaatat tgttttaagt ggtgtttgta gtaaatgtac
      901 aaactgttag tttaagagtt tctaactatt aaactagaag gaagattgaa ttgttttata
      961 agttcttctt ccttttgtct atgttcccca ttatcaactt cgtggtcata ccctaaaaga
     1021 tgcaaaagac cgtgaataaa aagtaaattt aactcatctt cttcactatg cccatactct
     1081 tttgccttct cttttacaaa atctattgaa attataatag ttcctaaagg taaatgttca
     1141 aaatcaccat caacaggaaa gcttaaaaca tctgttgctt catctttttt tctgtgttca
     1201 aggtttatct cttttattgt ttgattatct acaatgatta actctaaatc tttttttgta
     1261 agagaggtaa ctattttttc aagtgctact aaattcacca aaaaatctgt ttgattttca
     1321 aaatctatca ttttttatcc taaattaata tatttttaat agtatataac ttttaaaata
     1381 aatccaagga gagatgatga gtttaactgt agggaaaaag gcatcaattg attataaagt
     1441 tgagaaaaaa gatttagcat caaatctaaa tatatcagct gatttaactt ttccagaagt
     1501 ttttgcaaca gctagaatga tagctttaat ggagtgtagt gcagcaaaat taatgttacc
     1561 actattaaaa gatgatgaaa aaagtgttgg tgtaaatgta aatattacac atatggcagc
     1621 aactttagaa aatgatgtag ctatttcaac agcaactttt gttggaatgg aagggaaact
     1681 ttataaattt gagattgaag taattgatag tgcaggagtt tgtggaagag gaacacatac
     1741 aagagctatt gtttcaactt caagacttat tgaaggtgca caaagaagag ct
//
LOCUS       DAOQAN010000255         1792 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_100167_length_1792_cov_2.118595, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000255 DAOQAN010000000
VERSION     DAOQAN010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100167_length_1792_cov_2.118595"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..1184)
                     /gene="hemW"
                     /locus_tag="PKO10_04635"
                     /pseudo
     CDS             complement(97..1184)
                     /gene="hemW"
                     /locus_tag="PKO10_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012887.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
     gene            1391..>1792
                     /locus_tag="PKO10_04640"
     CDS             1391..>1792
                     /locus_tag="PKO10_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66464.1"
                     /translation="MLITLLLVKTNKPIYASAVDFYNVLKVSSNIPVNFFAINDYYYI
                     TSERNKELFVILFQIQKLSDYIIAGFGYIYTMYLLWNLCFKRIINVVKSKIIYFLMIL
                     SCLSPAILFLIACDYGRWYIYIINAFYTLAMF"
BASE COUNT          603 a          259 c          175 g          755 t
ORIGIN      
        1 taatatctca acctcttttg ctggaatatg tgtgatattt tataaatata ctgtgtattt
       61 ttttacacac tcttttattg tcattttacc tcttttttaa tctaaaatat atagcgctat
      121 ttcatctgca agtagaaaat tattattata aaccctattt tcttttatac aaaccctatt
      181 ttcaactact aaatcatcta cttttttaag ctctttagaa gatagtatat ttaaatcaac
      241 tccaaactta catctaaatc ctaaagtact ttttcagctt ttatatcatc actacttaac
      301 tcttcaatat cttcataaat tggattttgt atatattttt ctatattttt ttgtttatac
      361 attcttctat tttcaataca agcaacagct ccagctccaa ctcctatata atcatcttta
      421 gaccaatatc catagttatg ttttgattca aatttttcgt tagtactaaa atttgctatc
      481 tcatattgat taaagcctat atttttcaag tagttaaata tttcataact taaatcttca
      541 tcatcaattt ttaagctttt tttacttttt tgaagctctt tttcatttaa aaagaatttt
      601 gtatcctctt ctattattaa actatatgca cttaaatgtt caacttctag ctcttttaaa
      661 gtgtcaaaat ctctttttaa aagctcaaaa gtatcatttg taactccata tattatatcg
      721 caattaatac tattaaagcc tatacatttt gcattttgta tcgctttaat agcattaagt
      781 ctattgtgat ttctacctaa gaactttaac ttctcatcat caaaactttg tactccaaag
      841 ctaactctat ttactccaaa actatacatt ttttccaacc actcttttgt ggcactattt
      901 ggatttgctt cagttgtaat ttcacaattt tctaaaatat gtggatttat taatttaaaa
      961 atatcttcat ataaagaagc atcaacagtt gatggagtcc caccacctat aaaaacggta
     1021 tttaacttta tatctttatc ttttaaatag tttaaattgt actctaaatc aatttttaaa
     1081 gctctcatat atgcctcttt ttgactattt ttatttgtat atgagttaaa tgcacagtaa
     1141 aaacatttgc tgtcacaaaa gggaatatgt atatataaaa gcaattttta atcctatatt
     1201 ttataaaata acgcctaatt ataaggaaaa atatatttat agtatacctg ttatatcaat
     1261 actgggtgta cttatacatg aaaactattt agtttggctt actccattaa tatgtatgtt
     1321 attaatttat aaatatttta atactaaaga taagaaattc atgtacgtac tatttttaaa
     1381 tgctattacg atgttaataa cattattatt agtcaaaact aataaaccta tatacgcaag
     1441 tgctgtagat ttttataatg tattaaaggt aagtagtaat attccagtta atttttttgc
     1501 tattaatgat tactactata ttacaagtga aagaaataaa gaactttttg taatattgtt
     1561 tcaaatacaa aaacttagtg attatataat tgccggattt ggatacatat atactatgta
     1621 tttgttatgg aatttatgtt ttaagagaat aataaatgtt gttaaaagta aaattattta
     1681 ttttcttatg attttatcgt gtctatctcc agccatatta tttctaatag cctgtgatta
     1741 tggtagatgg tatatatata taataaatgc attttatact ttggcaatgt tc
//
LOCUS       DAOQAN010000256         1791 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_100245_length_1791_cov_2.135369, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000256 DAOQAN010000000
VERSION     DAOQAN010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100245_length_1791_cov_2.135369"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /gene="nadD"
                     /locus_tag="PKO10_04645"
     CDS             complement(<1..351)
                     /gene="nadD"
                     /locus_tag="PKO10_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HNW66465.1"
                     /translation="MKIAIFGGSFDPIHIAHKKIVETALKELDIDKLIIVPTYLNPFK
                     KDFLFEPKERFKLLQKVFKDEKRVEICDFEINQKKLSYTYETIRYIKASINPSKIYFI
                     IGEDNLKSLNRWHNI"
     gene            442..1440
                     /gene="gap"
                     /locus_tag="PKO10_04650"
     CDS             442..1440
                     /gene="gap"
                     /locus_tag="PKO10_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014475312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HNW66466.1"
                     /translation="MAIKVAINGFGRIGRCVARIIATRSDIELVAINDTAEASMLEYI
                     TKYDTVHGTFEGDVKVENGFLKMGKINAKLYSTRDAKELSFAKDCGAEIVLECTGAYL
                     TQDKCQVHIDNGASKVVMSAPAKDDTKTFVVGVNESTYNGEKIISNASCTTNCLGPIA
                     KIIDDAFGIEKGLMTTIHSYTNDQNILDVKHKSDKRRARAGAANMIPTSTGAAKAMKL
                     IMPQLDGKLHGQSVRVPTPNVSMVDVNFLIKKDTTKEEINALFTQKSKELSGIVAVDN
                     DMLVSSDLIGNTNSTIIASDLTQVIGGNMIKVMSWYDNEWGYSARLVDLAIYVAKK"
     gene            1449..>1791
                     /gene="pgk"
                     /locus_tag="PKO10_04655"
     CDS             1449..>1791
                     /gene="pgk"
                     /locus_tag="PKO10_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNW66467.1"
                     /translation="MKLQEIKNIDITGKKVFIRCDFNVPVDEYNNITDDRRIRSALNT
                     IRYCIDNDCSVILASHFGRPKGGFEEKYSLSPIAKRLHILLKQDIKLAPNVVCTETLK
                     MANELKTGEIML"
BASE COUNT          677 a          218 c          300 g          596 t
ORIGIN      
        1 tatgttgtgc catctattaa gagattttag attatcttct cctataataa agtagatttt
       61 actaggattt atcgaagctt taatatatct tattgtttca taagtgtagc ttagtttttt
      121 ttgatttatc tcaaaatcac aaatctcaac tcttttttca tctttaaaaa ctttttgtaa
      181 gagtttaaat ctctcttttg gttcaaataa aaaatctttt ttaaaaggat taagatatgt
      241 aggaacaatt attaatttat caatatctag ctcttttaaa gcagtttcaa caatcttttt
      301 atgagcaata tgaattgggt caaaactacc accaaaaatc gcaattttca aaaaaaactt
      361 acctttgttt tatgattaaa ttatattata gcaattttta gataaaatat ttgccattta
      421 gaattttaat aaagggatta gatggctatt aaagttgcaa taaacggatt tgggagaata
      481 ggaagatgtg tagctagaat tattgctaca agaagtgaca tagagttggt tgctataaat
      541 gatacagcag aagcttcaat gcttgaatat atcacaaaat atgatactgt tcatggaact
      601 tttgaaggtg atgttaaagt tgaaaatggt ttcctaaaaa tggggaaaat caatgcaaaa
      661 ctttattcaa caagagatgc aaaagagtta tctttcgcaa aagattgtgg agctgaaatt
      721 gttttagagt gtacaggtgc atatttaact caagataaat gccaagttca tattgacaat
      781 ggtgcttcaa aagttgttat gagtgctcct gcaaaagatg atacaaaaac ttttgttgta
      841 ggtgtaaatg agtcaactta taatggagaa aaaattatat caaatgcatc ttgtactaca
      901 aactgtcttg gaccaatagc aaaaataatt gatgatgctt ttggaataga aaaaggttta
      961 atgacaacta ttcactctta tactaatgac caaaacatcc ttgatgtaaa acataaatct
     1021 gataaaagaa gagctcgagc aggtgctgct aatatgattc ctacaagcac tggtgctgca
     1081 aaagctatga aattaataat gcctcaactt gatggtaaat tacatggtca aagcgtaaga
     1141 gttccaactc caaatgtttc aatggtagat gtaaatttct taatcaaaaa agatactaca
     1201 aaagaagaaa ttaatgcttt atttactcaa aaatcaaaag agctttctgg aatagtagcg
     1261 gttgataatg atatgctagt ttcaagtgat ttaataggaa atacaaactc tacaattatt
     1321 gcaagtgact taactcaagt tattggtgga aatatgatta aagttatgag ctggtatgac
     1381 aatgagtggg gatattcggc tagacttgta gatttagcta tttatgtagc aaaaaaataa
     1441 ggaataatat gaaacttcaa gagataaaaa acatagatat tactggtaaa aaagtattta
     1501 taagatgtga ttttaatgtt cctgttgatg aatacaacaa tataactgat gatagaagaa
     1561 ttagaagtgc tctaaacaca attagatatt gtattgataa tgattgctct gttatattag
     1621 ctagtcactt tggaagacca aaaggtggat ttgaagagaa atattcactt tctccaatag
     1681 caaaaagatt acatatttta ttaaaacaag atatcaaact agctccaaat gtagtttgta
     1741 ctgaaacttt aaaaatggct aatgagttaa aaactggtga aattatgctt t
//
LOCUS       DAOQAN010000257         1790 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_100328_length_1790_cov_2.333141, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000257 DAOQAN010000000
VERSION     DAOQAN010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100328_length_1790_cov_2.333141"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            50..565
                     /locus_tag="PKO10_04660"
     CDS             50..565
                     /locus_tag="PKO10_04660"
                     /inference="COORDINATES: protein motif:HMM:NF017394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HNW66468.1"
                     /translation="MRKKILTSMLLLSTTLFAMDHSNHNMQHHNTNEVANQNSSLTIP
                     KDAKCPVCGMFVAKYEKWIATIKTEKESFYFDGVKDMMKFYFEPTKYIKNADLKNSTI
                     KVTDYYTLEQIDAKNAFFVIGSNVMGPMGDELIPFKDENSANEFSKDHFGKKIVKFNE
                     ITLDTIPKKHH"
     gene            complement(595..909)
                     /locus_tag="PKO10_04665"
     CDS             complement(595..909)
                     /locus_tag="PKO10_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HNW66469.1"
                     /translation="MSYFKGVSIAKAANILYDGNITSRAITFEDGSKKTLGIMLAGEY
                     ELNTVNKEVIDIQRGKIDLLLPASDWQEFEGPASFTIPANTKFRLRVHSLVDYCCSFI
                     KD"
     gene            1024..1287
                     /locus_tag="PKO10_04670"
     CDS             1024..1287
                     /locus_tag="PKO10_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66470.1"
                     /translation="MSIQKELKEIINNEVLVEIEDHIDDIFEIIAAKKDTPELKEELE
                     NMQEMKKDFEELLKDLEAGEIDDEEAQEIYDEIVDMLEGSNED"
     gene            1303..>1790
                     /gene="recO"
                     /locus_tag="PKO10_04675"
     CDS             1303..>1790
                     /gene="recO"
                     /locus_tag="PKO10_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecO"
                     /protein_id="HNW66471.1"
                     /translation="MQGYIVDIKAVKDDDLIVTIICENELITSYRFYGARHSNINIGY
                     KIDFELETTKSTISRLKDVIQLGFPWILNNQKMYAWQRYLKLFYAHLKELHELEPFYF
                     FLLENLVSKIEKQNVYRAIIESYLSILEHEGRLHTDFECLICEVEINSDLSIVRGFLP
                     VH"
BASE COUNT          711 a          223 c          269 g          587 t
ORIGIN      
        1 aattaactca tttttcatat acttatacat tttaaaataa agggaaatta tgagaaaaaa
       61 aattcttact tcaatgttac ttttgagtac aacacttttt gcgatggatc actctaacca
      121 taatatgcaa catcacaata caaacgaagt ggctaatcaa aatagtagtt taactattcc
      181 aaaagatgct aaatgtcctg tttgtggtat gtttgtagca aaatatgaaa aatggattgc
      241 aacaattaaa acagaaaaag agagtttcta ttttgatgga gtaaaagata tgatgaagtt
      301 ctactttgaa ccaacaaaat atataaaaaa tgctgattta aaaaattcaa ctataaaagt
      361 tactgattac tacactcttg agcaaataga tgcaaaaaat gctttttttg taattggttc
      421 aaatgttatg ggacctatgg gagatgaatt aataccattt aaagatgaaa atagtgcaaa
      481 tgagttttca aaagaccatt ttggaaagaa aattgtgaaa tttaatgaaa taactttgga
      541 tacaatacca aaaaaacatc actaaacaat gcgttttcgc attgtttaaa tagattaatc
      601 ttttataaat gagcaacaat aatcaactaa actatgtact cttaatctaa actttgtatt
      661 tgcaggaata gtaaaagatg caggaccttc aaactcttgc caatcagaag caggaagtaa
      721 taaatcaatc tttcctcttt gaatatcaat aacttcttta tttacagtat taagctcata
      781 ctcacccgct aacataattc ctaaagtttt tttgcttcca tcttcaaatg taatagcacg
      841 gcttgtaata tttccatcgt ataaaatatt tgctgctttt gctatagaaa cacctttaaa
      901 atatgacatt atagtctcct taaaatttat cgcagtatta tatcaaatca attatacgat
      961 attattgaag caaaaggcta gaaatctttt gatataattc aaaaaattaa ataaggtgag
     1021 aaaatgagta tccaaaaaga gcttaaagag ataataaata atgaagtttt agttgaaata
     1081 gaagatcaca tagatgatat ttttgaaata attgctgcaa aaaaagatac tcctgagtta
     1141 aaagaagagc ttgaaaatat gcaagagatg aaaaaggatt ttgaagaact tttaaaagat
     1201 ttagaagctg gtgaaataga tgatgaagag gctcaagaga tttatgatga aatagttgat
     1261 atgcttgaag gttcaaacga agattaataa taagattaag atgtgcaagg ctatattgtt
     1321 gatataaaag ctgtaaaaga tgacgattta attgtcacta ttatatgcga aaatgagttg
     1381 attacttcat atagatttta tggagcaaga cattcaaata tcaatattgg ctataaaata
     1441 gactttgaac ttgaaactac aaaatctact atttcaagac ttaaagatgt gattcaacta
     1501 ggtttccctt ggattttaaa caatcaaaaa atgtatgctt ggcaaagata tcttaaactt
     1561 ttttatgctc acttaaaaga gcttcatgag ttagaaccat tctacttttt tcttttagaa
     1621 aatttagttt caaaaataga gaaacaaaat gtttacagag ctattataga gtcatatcta
     1681 tcaattttag aacatgaagg aagacttcat acagattttg agtgtcttat ttgtgaagtt
     1741 gaaataaata gtgatttatc aatagtaaga ggttttttac ctgttcacaa
//
LOCUS       DAOQAN010000258         1789 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_100398_length_1789_cov_2.788927, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000258 DAOQAN010000000
VERSION     DAOQAN010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100398_length_1789_cov_2.788927"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /locus_tag="PKO10_04680"
     CDS             <1..673
                     /locus_tag="PKO10_04680"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNW66472.1"
                     /translation="KNSLKEDDFTYTKQIGFGGDNSLKIDIFFEDIFIKNLKDFGNIF
                     SEECGFIDNKKDITFIIDPLDGSNNFLSNIPYFGTSVAVKKNGEIIAGFVANMANKTL
                     VYRILNDEVKYFCLENRKEIQKIVHNISKVAVFERGYKYPNICKNIDDKNIKFRVLGA
                     TALSLANARDFLFVIFKGDLREFDLEAGLFISKDLYIKKEEDIIFVTKYKEQFSIFKE
                     IIKDF"
     gene            712..966
                     /locus_tag="PKO10_04685"
     CDS             712..966
                     /locus_tag="PKO10_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014475006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HNW66473.1"
                     /translation="MALIIMDECIACDACREECPNMAIEEGDPIYVIDADRCTECVGH
                     YEEPQCVEVCPVDCIILDPDNEETLEELKFKYEQLMEEEN"
     gene            968..>1789
                     /locus_tag="PKO10_04690"
     CDS             968..>1789
                     /locus_tag="PKO10_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014469203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HNW66474.1"
                     /translation="MSKITTVIDIGSNSMRMVVLEKSSRFAFSLINETKSRVKISEGC
                     YENGGNLQEIPMERAYQSLKSFLNISNALKSRKVLCVATSALRDAPNSKVFLNRVKND
                     LGLSIKVIDGEKESYFGGVATSNLLHDDEFITIDIGGGSTEFCFVKDKKILKSISLNI
                     GTVRIKELYFNKNNIKGAKDYILENLKKVFEQNIDIPSKAVGIGGSIRTLSKMIMDKN
                     EYPLDAIHGFTYNVKAEKKLFENILKADTNDELQELGVKKDRFDTIKEGTFIFKTI"
BASE COUNT          709 a          151 c          321 g          608 t
ORIGIN      
        1 aaaaaatagt ttaaaagaag atgattttac atatacaaaa cagattggat ttggtggaga
       61 taattcacta aaaattgata ttttttttga agatatattt ataaaaaatc taaaagattt
      121 tggaaatatt tttagtgaag agtgtggatt tattgataat aaaaaagata ttacatttat
      181 aatagaccct cttgatggaa gcaataattt tttatcaaat attccatatt ttggcacttc
      241 agttgcagtt aaaaaaaatg gtgaaattat tgctggtttt gtagcaaata tggcaaacaa
      301 aactttagtt tatagaattt tgaatgatga ggtaaaatat ttttgtttgg aaaatagaaa
      361 agagatacaa aaaatagttc ataatatttc aaaagttgca gtttttgaaa gaggatataa
      421 atatccaaat atttgtaaaa atatagatga taaaaatata aagtttagag ttcttggtgc
      481 aacagcacta tctttggcaa atgcaagaga ttttttgttt gtgattttca aaggagattt
      541 aagagagttt gatttagaag ctggactttt tataagtaaa gatttatata taaaaaaaga
      601 agaagatatt atatttgtta caaaatataa agaacaattt tcaattttta aagaaattat
      661 taaagatttt taggtataac tccagattaa taaaaaaatt aggaaaatta tatggcttta
      721 ataattatgg acgaatgtat tgcatgtgac gcttgtaggg aagagtgtcc aaatatggcg
      781 attgaagagg gtgatccaat ttatgtaatt gatgctgatc gttgtacaga gtgtgttggg
      841 cactatgaag aacctcagtg tgtagaggtt tgtccagttg attgtattat tttggatcca
      901 gataatgaag agacactaga agagctaaaa ttcaagtatg aacagttaat ggaagaagaa
      961 aactaaaatg tcgaaaatta caacagtcat cgatattggc tcaaactcaa tgcggatggt
     1021 tgttttagaa aaatctagcc gttttgcttt ttctttaata aatgagacaa aaagtagagt
     1081 aaaaatttct gaaggttgct atgaaaatgg tgggaacctt caagaaattc ctatggaaag
     1141 agcttatcaa tctttaaaat cttttttaaa tatttcaaat gctttaaaat caagaaaagt
     1201 tttgtgtgtg gcaacttctg ctttaagaga cgctccaaat tcaaaagttt ttttaaatag
     1261 agttaaaaat gatttgggat tgagtataaa agtaattgat ggagaaaaag agtcttattt
     1321 tggtggtgtt gctacttcaa atttacttca tgatgatgag tttataacta ttgatattgg
     1381 tgggggaagt acagaatttt gttttgtaaa agataaaaaa atcttaaaat caatatcttt
     1441 aaatattgga actgtaagaa taaaagagtt gtattttaat aaaaacaata tcaaaggtgc
     1501 aaaagattat attttagaga acttaaaaaa agtttttgaa caaaatattg atattccttc
     1561 aaaagctgtt ggaattggtg gaagtattag aactttatca aaaatgatta tggataaaaa
     1621 tgagtatcca ttagatgcaa ttcatgggtt tacatataat gtaaaagcag aaaaaaaact
     1681 ttttgaaaat attttaaaag cagatacaaa tgatgaactt caagaattag gggttaaaaa
     1741 agatagattt gatactataa aagagggtac atttattttt aaaactatt
//
LOCUS       DAOQAN010000259         1783 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_100884_length_1783_cov_3.818287, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000259 DAOQAN010000000
VERSION     DAOQAN010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100884_length_1783_cov_3.818287"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..136)
                     /locus_tag="PKO10_04695"
     CDS             complement(<1..136)
                     /locus_tag="PKO10_04695"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HNW66475.1"
                     /translation="MIKSNIFSGLTVGIIALPLSMALAIATGVPPQLGLYTAIVAGIL
                     A"
     gene            complement(180..818)
                     /locus_tag="PKO10_04700"
     CDS             complement(180..818)
                     /locus_tag="PKO10_04700"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNW66476.1"
                     /translation="MRNYILLPIFTIFLFSACSFKMPEFLTFSDINYDELLKEANICQ
                     DLDTQKEKLDCYKKIENSNSFAQIRLGTYYSTKNDYKESLKYLNMADENKNLYAKLPL
                     ALLYYKGEGVKKDINKSFELLKESSDIDPIAAFQLSRFYLQGINTKIDNEKGIDLLEF
                     SAQNGVLQAQELLSNVYKQGLYGVAKDQIKYEYWLNKATSNKEDKNSNIYIF"
     gene            complement(822..1436)
                     /locus_tag="PKO10_04705"
     CDS             complement(822..1436)
                     /locus_tag="PKO10_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014475419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HNW66477.1"
                     /translation="MLKVIKFSSIFIVLSIFTACAGHKKAMEVLPNELEQLNQDCKRD
                     SLAFELDCYDFIATKNSFAMLRLGIDAQNKGRPAEAFERYTKAQKAGNFYANALLSTL
                     YGNGLGVEFDEKKSINLLKDVEDVDPIAAYRLSYYYFSNGNPQKAIELLEYAATNEVK
                     DAQKDLVLVYTNGQYIEPNDEKALFYDNLYQDGKEDFTKKIYGR"
     gene            1554..1775
                     /locus_tag="PKO10_04710"
     CDS             1554..1775
                     /locus_tag="PKO10_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66478.1"
                     /translation="MQEVIDFLKAKNVEKTKFFNQLKCSLEEAKILQYLSKEYINGRD
                     VLSTIDILGQFYSIEKYEHRKTKFDKIFA"
BASE COUNT          625 a          254 c          231 g          673 t
ORIGIN      
        1 ctgctaaaat tcctgcaaca attgctgtat aaagaccaag ttgaggtgga actccagttg
       61 caattgcaag tgccatagat aaaggcaagg caataattcc aactgtaagc cctgaaaaga
      121 tattactttt tatcatcttt tattctttaa aataggattt gcgacctttt taaaaaattc
      181 tagaagatat aaatatttga gtttttatcc tctttattag aagttgcttt gtttaaccaa
      241 tactcatatt ttatctggtc ttttgcaact ccataaagtc cttgtttata gacatttgac
      301 aataactctt gagcttgtaa tactccattt tgagcagaaa actcaagtaa atctatacct
      361 ttttcattat ctatttttgt atttatccct tgaagataaa atcttgataa ctgaaatgct
      421 gcaatagggt caatatcgct agactctttt agaagttcaa aagatttatt gatatctttt
      481 ttaacccctt ctcctttgta gtataaaaga gctaatggca attttgcata gagattttta
      541 ttctcatcag ccatatttag gtattttaaa ctctctttat aatcgttttt tgttgaataa
      601 taagttccta gcctaatttg tgcaaaagag ttactatttt caattttttt ataacaatct
      661 aacttctctt tttgagtatc taaatcttga caaatatttg cttcttttag aagttcatca
      721 taatttatat ccgaaaaagt tagaaattca ggcattttaa aagaacaagc actaaaaaga
      781 aaaatagtaa aaattggtaa taaaatataa tttctcatct attatctacc ataaattttt
      841 tttgtaaaat cttcttttcc atcttgatat aaattatcat aaaaaagagc tttttcatcg
      901 tttggttcta tatattgacc atttgtataa actaaaacta aatctttttg agcatctttt
      961 acttcatttg tagcagcata ttcaagaagt tctatagctt tttgtggatt tccatttgaa
     1021 aaatagtagt aagataatct ataagctgca attggatcaa catcttcaac atcttttaga
     1081 agatttatag attttttttc atcaaattct acacctaaac cattaccata aagagttgat
     1141 aaaagtgcat ttgcatagaa attaccagct ttttgtgctt ttgtatatct ttcaaaggct
     1201 tcagcaggtc ttcccttatt ttgagcatca attccaagtc ttagcattgc aaaactattt
     1261 ttagttgcta taaaatcata acaatctaac tcaaaagcca agctatctct tttgcaatct
     1321 tgatttaact gctccaattc gtttggcaaa acttccatag cttttttatg accagcacaa
     1381 gcagtaaaaa tagataaaac tataaaaata gaagagaact taataacttt tagcataata
     1441 aaagtctcct ttaaaaataa atgtttgtat tttatcattt ttttattcat ttgtagttaa
     1501 gacgatttaa gataatatat atgcttaatt aaattaatat ttgtaagggt tttatgcaag
     1561 aggtaataga ttttttaaag gcaaaaaatg ttgaaaaaac gaaatttttt aatcagctaa
     1621 aatgtagttt agaagaagca aaaattttgc agtacctctc aaaagagtat ataaatggaa
     1681 gagatgtact aagtacaatt gatattttag gacagtttta tagtattgaa aaatatgaac
     1741 atagaaaaac taagtttgat aaaatctttg cttgagtatg gat
//
LOCUS       DAOQAN010000260         1783 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_100907_length_1783_cov_2.921296, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000260 DAOQAN010000000
VERSION     DAOQAN010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100907_length_1783_cov_2.921296"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            423..>1783
                     /locus_tag="PKO10_04715"
     CDS             423..>1783
                     /locus_tag="PKO10_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase B"
                     /protein_id="HNW66479.1"
                     /translation="MSLLQEYKAHEAQRQSEGGLPGLALTAAQTADLVEILKASKVED
                     AQYALNLFKNKINPGVDDAAYVKAAFLNDIVQGKVACSVISKVEAVEILGTMMGGYNV
                     PPLVEALKVAEVADAAAKELKNTILVYNSFNDVKSLMDSGNAKAKEVIESWANAEWFT
                     NKPALEEEITLTVYKIPGETNTDDLSPATVAFTRADIPLHATAMLQSRMEKPLEKMEE
                     LKAKGHPLAYVGDVVGTGSSRKSGINSVQWHMGRDIPGVPNKRTGGVVIGSIIAPIFF
                     NTAEDSGCLPIEANVDNLETGDVITVKPYAGEILKDGKVVSTFKLSPNTLTDEMRAGG
                     RIPLIIGKGLTAKAREALKLSASTAFIAPEQPANNGKGYTQAQKMVGRACGVEGVKPG
                     MYVEPIATTVGSQDTTGPMTRDEIKELAALSFGADMVMQSFCHTAAYPKPADIKLRHT
                     LPD"
BASE COUNT          666 a          252 c          314 g          551 t
ORIGIN      
        1 ataactttta gttcacttaa agactcttta tatgattttt cacccaaagc taactcttct
       61 tgtgatgaca taagaattag ttgagacctg tttgtatatg gagttttgtg tgtacaagct
      121 gaaaataaaa tagccacaaa tgtagcaaat ataacaattt taaaatgtct catttttttc
      181 ctctttgaaa taaaatttca ctatttaatc aaaaaaaagt ataaaaatag tttaattaaa
      241 tattgtacat aaattgtaca atatttaaga tactaaaatc tataaagtgt ttatataaac
      301 tacaagtgta acttttattt acaaaatata aatttgtaca actataaaca ataattgtac
      361 aaagatattt agaactttgt gatataattc acccaaattc ttaaaattta ataggagaaa
      421 aaatgagttt attacaagag tataaagcac acgaagcaca aagacaaagc gaaggtggat
      481 taccaggtct tgctcttaca gctgctcaaa ctgctgattt agtggagatt ttaaaagcta
      541 gtaaagttga agatgcacaa tatgcattaa atctatttaa aaacaaaatt aacccaggtg
      601 ttgatgatgc tgcttatgtt aaagctgctt ttttaaatga tatagttcaa ggtaaagttg
      661 catgttctgt tatttcaaaa gttgaagctg ttgaaatttt aggaacaatg atgggaggat
      721 acaatgtacc accacttgtt gaagcactaa aagttgctga agttgcagat gccgctgcta
      781 aagagttaaa aaatacaatt cttgtttaca actcatttaa tgatgtaaaa tcattaatgg
      841 attctggaaa tgcaaaagct aaagaagtta ttgaatcatg ggcaaatgct gagtggttta
      901 caaataaacc agctttagaa gaagagatta cattaacagt ttataaaatt ccaggtgaaa
      961 caaatacaga tgatttatct ccagcaactg ttgcatttac aagagcagat attccattac
     1021 acgcaactgc aatgttacaa tcaagaatgg aaaaaccttt agaaaaaatg gaagaattaa
     1081 aagcaaaagg tcacccttta gcatatgttg gtgatgttgt tggaactgga tcttcaagaa
     1141 aatcaggaat caactctgtt caatggcata tgggaagaga tattccaggt gttccaaata
     1201 aaagaacagg tggagttgta attggatcta tcattgctcc aattttcttt aacacagcag
     1261 aagattcagg atgtttacca atcgaagcaa atgttgacaa tctagaaact ggtgatgtta
     1321 tcacagttaa accatatgct ggtgaaattt taaaagatgg aaaagttgtt tctacattta
     1381 aattaagtcc aaacacttta acagatgaaa tgagagctgg tggaagaatt cctttaatta
     1441 ttggaaaagg attaactgca aaagctagag aagctttaaa attatcagct tcaactgcgt
     1501 ttattgctcc tgagcaacct gcaaacaatg gtaaaggtta tactcaagca caaaaaatgg
     1561 ttggacgtgc ttgtggtgtt gagggtgtta aaccaggtat gtatgttgaa cctattgcaa
     1621 ctactgtagg aagccaagat acaacaggac caatgactag agatgagatt aaagaacttg
     1681 ctgcattatc ttttggtgct gatatggtta tgcaatcatt ctgtcataca gctgcttatc
     1741 caaaaccagc agatattaaa ttaagacata ctttaccaga ttt
//
LOCUS       DAOQAN010000261         1783 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_100936_length_1783_cov_2.310185, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000261 DAOQAN010000000
VERSION     DAOQAN010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100936_length_1783_cov_2.310185"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1317
                     /locus_tag="PKO10_04720"
     CDS             <1..1317
                     /locus_tag="PKO10_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014469241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNW66480.1"
                     /translation="KVSDGAPVTSQVLAGAKGIVKYYKLTGDYLERRHDIKAGDKVVE
                     KGLFAVIADADDREAIRHYISRGSSIVLNDNSEVEKGTQISAPTTKEQTVIAEWDPYA
                     NPTIAEQSGIVTFEDIIPGVTVSEQFDELTGTSKLVVNEYIPAGYKPTVLLATASNEI
                     IRYVLDPKASLNIQEGKKVEIADIIAKTPKATQKSKDITGGLPRVSELFEARRPKNIA
                     ILASFDGIVTFGKPLRNKQKLIITDAVGNQTEYLVEKGKQILVHEGEFVHAGESLTDG
                     QVSPHDVLKILGEKALHYFIVSEVQQVYRSQGVNIADKHIEVITSQMLRQVSIIDGGD
                     TKFIMGDMISKKRFKIENEKIIRMGGKPAIAEPVLLGITRAAVTSDSIISAASFQETT
                     KVLTEAAISAKMDMLEDLKENVVIGRTIPVGTGLYKDQKIIFSEEK"
     gene            1343..1726
                     /locus_tag="PKO10_04725"
     CDS             1343..1726
                     /locus_tag="PKO10_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014469240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66481.1"
                     /translation="MEIVHFFFIFVAIELFESNWQKSSTLYGMLENNFLVYNKNIFLY
                     FILHISFFYSLYLSISSNNFGFWMSSILALKFFDIIFKLSIMKKLSDGININEIIPFD
                     ANITPILRYLNVLIYPLLFIFATTL"
BASE COUNT          672 a          220 c          298 g          593 t
ORIGIN      
        1 aaagtttcag atggagctcc tgttacttct caagttttag caggtgcaaa agggatagtt
       61 aaatactata aattgactgg tgattattta gaaagaagac atgatataaa agcaggtgat
      121 aaagttgttg aaaaaggact ttttgctgtt attgcagatg ctgatgatag agaagctata
      181 agacactata tttcaagagg ttcttcaata gttttaaatg ataatagtga agttgaaaaa
      241 ggtactcaaa tttcagcacc aacaactaaa gagcaaactg ttattgcaga gtgggatcca
      301 tatgcaaacc ctacaatagc agagcaatca ggtattgtaa catttgaaga tattattcca
      361 ggtgttactg tttctgagca gttcgatgag ttaacaggaa catcaaaact agttgtaaat
      421 gagtatatac cagctggata taaaccaaca gttttattag caactgcttc aaatgagatt
      481 ataagatatg ttttagaccc aaaagcatct ttaaatattc aagagggtaa aaaagtagaa
      541 attgcagata ttattgctaa aacaccaaaa gcaactcaaa aatcaaaaga tattactgga
      601 ggtcttccta gagtatctga gttatttgaa gctagacgac caaaaaatat tgcaatttta
      661 gcttcatttg atggaatagt aacttttgga aaaccgctta gaaataagca aaaactaatt
      721 attacagatg ctgttggaaa tcaaactgaa tatttagttg aaaaaggtaa gcaaatatta
      781 gttcatgaag gtgaatttgt tcatgctggt gagtctttga cagatggtca agtgtctcct
      841 cacgatgttt taaaaatttt aggtgaaaaa gctctacatt actttattgt atctgaagta
      901 caacaagtat atagatctca aggggtaaat attgcagata aacatattga ggtaattaca
      961 tctcaaatgt taagacaagt ttcgataatc gacggtggag atacaaaatt catcatgggt
     1021 gatatgattt ctaaaaagag atttaaaatc gaaaatgaaa aaataataag aatgggtgga
     1081 aaaccagcta ttgcagaacc agttttattg ggaatcacaa gagcagcagt tacatctgat
     1141 agtattattt cagctgcatc attccaagag actacaaaag ttttaacaga agcagcaatt
     1201 agtgcaaaaa tggatatgct tgaggattta aaagagaatg ttgtaatagg aagaacaatt
     1261 cctgttggaa caggacttta taaagaccaa aaaattatct tttcagaaga gaaataaata
     1321 gggcaaagcc ctatttataa agatggaaat agttcacttt ttttttatct ttgttgctat
     1381 tgaacttttt gagtcaaatt ggcaaaaatc ttctactctt tatggtatgc tagaaaataa
     1441 ttttttagta tataataaaa atatcttttt atattttatt ttacacatat cattttttta
     1501 ttcactttac ctatctatat catcaaataa ttttggtttt tggatgagtt caattttagc
     1561 tttaaaattt tttgatatta tttttaaact ttctattatg aaaaaactct cagatggtat
     1621 aaatataaat gaaataatac cttttgatgc aaatattacg ccaattctta gatatttaaa
     1681 tgtattaatt tatccgctac tttttatttt tgctactact ttataaattt tgtgataaaa
     1741 taattaaaaa tttaaaagga taaaaatgca atttttaata ata
//
LOCUS       DAOQAN010000262         1780 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_101164_length_1780_cov_2.992464, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000262 DAOQAN010000000
VERSION     DAOQAN010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101164_length_1780_cov_2.992464"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1780
                     /locus_tag="PKO10_04730"
     CDS             <1..>1780
                     /locus_tag="PKO10_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014475492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNW66482.1"
                     /translation="AGNSRIKITFSQEVNADIAQVQVQNKVNQILSRLPEDVQRQGVR
                     VFKSQSDFLMMASVYDSTGLADKTDISDFLVSNLQDSISRIEGVGDVQVFGGQYAMRI
                     WLDPYKLEKYKLIPKDVENAINAQNSQASAGRLGAMPTLETQQLSVVVTARSMFRNVG
                     QFENIVLKSNLDGSVVKIKDIARVEIGAQSYSNVTALNGFPASGISIQLASGANAVAT
                     SNRVKEFLAQSENMLPQGYKIAYPRDTTSFIEASINEVVKTLVEAIVLVILVMFLFLK
                     SFRATLIPAIAVPVVLLGTFAILNIFGYTINTLTMFALVLAIGLLVDDAIVVVENVER
                     NMNEKGLNPKEATILSMQEVTSALIGITTVLSVVFLPMAFFSGSTGIIYRQFSITIIS
                     SMVLSVIVALTLTPALCSTILKPHKKNEDKTQEKNSGFFFWFDTKFENFTNKYKFWVE
                     KLLYSQKKWIIFYVVIIISSTYIFIKLPTSFLPKEDQGSLMIQYTLPVGAVASRTVDV
                     ANVVRDYFMIEEADALNTIFTISGFSSRSSGQNVGTAYVSLKNWDLRDKSNHVDEISK
                     RATKAFNDPNSKYFIRDARVFAINPSV"
BASE COUNT          625 a          215 c          326 g          614 t
ORIGIN      
        1 tgcaggaaac tctagaataa aaataacttt ttctcaagaa gtaaatgccg atattgctca
       61 agttcaagta caaaataaag taaatcaaat attatcaaga cttccagaag atgtacaaag
      121 acaaggtgta agagttttta aatctcaaag tgatttttta atgatggcat ctgtgtatga
      181 ttcaactggt ttggctgata aaacagatat tagtgatttt ttggtaagta atcttcaaga
      241 tagtatttca agaattgaag gtgttggaga tgttcaggtt tttggtggtc agtatgctat
      301 gagaatttgg ctagatccat ataagctaga aaaatataaa ctaatcccaa aagatgtaga
      361 aaatgctata aatgctcaaa actcacaagc aagtgctgga agattaggag caatgcctac
      421 tttggagacc caacaactct ctgtggtggt tactgcaaga agtatgttta gaaatgttgg
      481 tcaatttgaa aatattgttt tgaaatcaaa tttagatgga agtgttgtaa agataaaaga
      541 tattgctaga gttgaaatag gggctcaaag ttatagtaat gttacagctt taaatggttt
      601 tccagcatct ggaatatcta ttcagcttgc aagtggagca aatgcggtag ccacttcaaa
      661 tagagtaaaa gagtttttag ctcagagtga aaatatgttg ccacaaggtt ataaaatagc
      721 ctatcctaga gatacaacaa gttttataga agcttcaata aatgaagttg taaaaacttt
      781 agtagaagca attgttttag taattcttgt aatgttttta ttcttaaaaa gttttagagc
      841 tactcttatt cctgctattg cagttcctgt tgtgcttttg ggaacttttg ctattttaaa
      901 tatttttggt tatacaataa atactttaac aatgtttgct ttggttcttg caattggtct
      961 tttggtagat gatgctattg ttgttgttga aaatgttgaa agaaatatga atgaaaaagg
     1021 tctaaatcca aaagaggcaa ctattctttc tatgcaagag gtaaccagtg cattaatagg
     1081 aattacaact gttttatctg ttgtattttt acctatggca ttttttagtg gaagtacagg
     1141 gataatttat agacaatttt ctattacaat tatctcatct atggttttat cagtaattgt
     1201 tgctttaact ttaactcctg ctttatgttc aactatttta aaaccacata aaaaaaatga
     1261 agataaaacg caagaaaaaa atagtggctt tttcttttgg tttgatacaa aatttgagaa
     1321 ctttacaaac aaatataaat tttgggttga aaaattactc tatagtcaaa aaaagtggat
     1381 aatcttttat gttgtgataa tcatatcttc tacatatatt tttataaaac tacctactag
     1441 ttttttacca aaagaggatc aaggaagttt gatgatacag tacaccttgc ctgttggtgc
     1501 ggttgcttct agaacagttg atgttgcaaa tgttgtaaga gattatttta tgatagaaga
     1561 ggcagatgct ttaaatacta tatttacaat ctctggattt agttcacgaa gtagtggaca
     1621 aaatgtagga actgcttatg tatcattaaa aaactgggat ttaagagata aatcaaatca
     1681 tgtagatgag ataagtaaaa gagctacaaa agcttttaat gacccaaata gtaaatattt
     1741 tataagagat gcaagagttt ttgctataaa tcctagtgtt
//
LOCUS       DAOQAN010000263         1776 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_101524_length_1776_cov_2.904707, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000263 DAOQAN010000000
VERSION     DAOQAN010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101524_length_1776_cov_2.904707"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            121..579
                     /locus_tag="PKO10_04735"
     CDS             121..579
                     /locus_tag="PKO10_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNW66483.1"
                     /translation="MKVSKKTDYALRALFAIAEADSLISIRELSEQIDVPRRFLENIM
                     LEMNKAGWVKSIPGRYGGYVLAKNSDEITMGEVIRYFEGMIAMISCVSVSSYEPCSQE
                     GKCYFRRVFLNIRNLTAQILDKTTIASCLSQAPVTKEDVLKEEFVGGLGI"
     gene            920..>1776
                     /locus_tag="PKO10_04740"
     CDS             920..>1776
                     /locus_tag="PKO10_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012148126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNW66484.1"
                     /translation="MIKNIKNIALASLLISTLSFAKDYDYKAEADKKSIEILNVSYDP
                     TREFYEDYNKNFAKYWQEKSGQKVTIKQSHGGSGKQARAVIDGLKADVVTLALAYDID
                     AISEKANLFPNDWQKKLEYNSSPYTSTIVFLVRKGNPKGIKDWNDLIKDGVEVITPNP
                     KTSGGARWNYLAAYAYGLKQELGSLDKIDFNSQKYKVADEKAKEYVSKLLKNVPVLDS
                     GARGATNTFVQRKIGDVLLAWENEAFLAINELGKDEFEIVVPSVSILAAPPVTVVNKN
                     AKKASFSQA"
BASE COUNT          688 a          191 c          306 g          591 t
ORIGIN      
        1 aagccgtagc tccttggtaa aaattaatat aaagagagaa gattttcttc tctccttttt
       61 ttatatcttg acacacaaat aaaaatcaaa tataatttat caattaggta ggtgataaat
      121 atgaaagtat caaaaaaaac agattatgca ttaagagcat tattcgcaat agcagaagct
      181 gatagtttaa tctctattcg agaattatca gagcagattg atgttccaag aagattttta
      241 gaaaatatta tgcttgaaat gaacaaagct ggatgggtaa agagtattcc aggaagatat
      301 ggcggttatg ttttggcaaa aaattcagat gagataacta tgggtgaggt tatacgatat
      361 tttgaaggta tgattgctat gatatcttgt gtttcagtct ctagttatga gccatgtagc
      421 caagaaggga aatgttattt tagaagagtt tttttaaata ttagaaattt aacagcacaa
      481 attttagata aaactactat tgcctcttgt ttaagtcaag ctcctgttac aaaagaagat
      541 gttttaaaag aagagtttgt tggtggtttg ggtatttaaa aaatagattt tgtaatattt
      601 ttcttttttg tagattaaaa ttttttaaat tttaatctac ttttataaaa ttagattcta
      661 tgaaatatcg attttatagc tattgttaac aaaattataa aaatatttta aaaatctctt
      721 gacaaatatt ttttttatca ttataatttc accagtttag taggtgatag ataaatttca
      781 cctttattga aaggataaaa aatgatgtgc gacatgtata aaataatata taaatcagaa
      841 aaaagaaaat aggtgagatt tttttaaaat ctttaattat catacattta gtgtgtatta
      901 aatttaaaag agagataaaa tgataaaaaa tataaaaaac atagcgttag catcattgct
      961 aatatcaaca ttaagctttg caaaagatta tgattacaaa gcagaagcag ataaaaaatc
     1021 tattgagatt ttaaatgtat cgtatgaccc aacaagagag ttttacgaag attataataa
     1081 gaattttgca aaatattggc aagaaaaaag tggacaaaaa gtgacgataa aacagtcaca
     1141 tggaggttca ggtaaacaag cacgagcagt aattgatgga ttaaaagctg atgttgtaac
     1201 tttagcattg gcttatgata tagatgcaat tagtgaaaaa gcaaatttat ttccaaatga
     1261 ttggcaaaaa aaattagagt ataactcttc tccttatact tcaactattg tatttttagt
     1321 tagaaaagga aatccaaaag gaattaaaga ttggaatgat ttaataaaag atggtgttga
     1381 agttattact ccaaatccaa agacttcagg aggtgcaaga tggaactatc ttgctgctta
     1441 tgcttatgga ctaaaacaag agttaggaag tttagataaa attgatttta actcacaaaa
     1501 atataaagtt gctgatgaaa aagcaaaaga gtatgtttca aaacttttaa aaaatgttcc
     1561 agttcttgat tcaggtgcta gaggagcaac taatacattt gtccaaagaa aaattgggga
     1621 tgttttacta gcttgggaaa atgaagcctt tttagcaata aatgaattag ggaaagatga
     1681 gtttgaaata gttgttcctt ctgtttcaat attagctgca cctcctgtta ctgttgtaaa
     1741 taagaatgct aaaaaggctt cattttccca agctag
//
LOCUS       DAOQAN010000264         1774 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_101749_length_1774_cov_2.246073, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000264 DAOQAN010000000
VERSION     DAOQAN010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101749_length_1774_cov_2.246073"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..771
                     /gene="cysW"
                     /locus_tag="PKO10_04745"
     CDS             <1..771
                     /gene="cysW"
                     /locus_tag="PKO10_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012148123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HNW66485.1"
                     /translation="IMLVVPLITIFVEAFRKGYEAYFLSFNDEYVLSAIKLTFITAAI
                     AVPLNVIFGICASWAIAKYSFFGKSFLITLIDLPFAVSPVISGFVYILLFGAQGWFGH
                     YLVENDIQIIFAVPGIVLATIFVTFPFVARELIPLMQEMGNDEEQAALLLGASGFQTF
                     LKVTLPNIKWGLLYGVILCNARAMGEFGAVSVVSGHIQGVTNTMPLQVEILYNEYNFV
                     AAFAVSTLLAILALLTLVLKYILEWKVQRKIKKLDNEQ"
     gene            787..>1774
                     /locus_tag="PKO10_04750"
     CDS             787..>1774
                     /locus_tag="PKO10_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012148122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter ATP-binding protein"
                     /protein_id="HNW66486.1"
                     /translation="MKIEVKNLTKYFGNFKALENINLDIVEGELLALLGPSGSGKTTL
                     LRMIAGLETVDSIDKGEILFNNIDVAKKDIKDRDIGFVFQHYALFRHMTVFENIAFGL
                     RVKPKKERVNNKEIEEKVTKLLKLIQLDGFASRFPWQLSGGQRQRVALARALAVEPKV
                     LLLDEPFGALDAKVRTDLRRWLKELQEELGITTILVTHDQEEALEVANRIVVINKGKI
                     EQIGTPEDVFHNPKNEFVINFLGNVNLFHAREIEDGANLEDITDNKYLIRPHELEIVD
                     INDSEAIIHAKVKYIQLAGSFVKVELTYLNDEKRVVLVELAHYEFSDKNIKKG"
BASE COUNT          654 a          203 c          333 g          584 t
ORIGIN      
        1 attatgcttg tagttcctct gattacaatt tttgtagaag cttttagaaa aggttatgaa
       61 gcatattttt taagttttaa tgatgagtat gtattaagtg caataaaact tacatttatc
      121 acagcagcca ttgcagttcc attaaatgtt atttttggta tttgtgcatc ttgggcaata
      181 gcaaaatact ctttttttgg taaaagtttt ttaataactt taattgattt gccttttgct
      241 gtaagtcctg ttatttctgg atttgtatat attttacttt ttggagcaca aggttggttt
      301 ggacactatt tagtagaaaa cgatattcaa ataatttttg cagttcctgg aattgtactt
      361 gcaactatat ttgtaacatt tccatttgtg gcacgtgagc taattccttt aatgcaagag
      421 atgggaaatg atgaagaaca agctgcactt cttttgggcg ctagtggatt tcaaacattt
      481 ttaaaagtta ctttaccaaa tataaaatgg ggattacttt atggagtaat attatgtaat
      541 gcaagagcaa tgggagagtt tggagcagta tcagttgtat ctggacatat tcaaggtgtt
      601 acaaacacaa tgccacttca agttgagatt ttatacaacg aatacaactt tgttgcagca
      661 tttgcagttt caacactttt agcaatattg gcacttttaa ctcttgtttt aaaatatatt
      721 ttagagtgga aagtacaaag aaaaattaaa aaattagata atgaacaata aagcaggaaa
      781 taaaaaatga aaatagaagt aaaaaattta acaaaatatt ttggaaattt caaagcacta
      841 gaaaatatca atttagatat tgttgaaggt gaattattag cacttttagg accatcagga
      901 agtggaaaaa caacactttt aagaatgatt gcaggacttg aaacggttga tagcatagat
      961 aaaggtgaga tattatttaa caatattgat gtggctaaaa aagatattaa agatagagat
     1021 atagggtttg tatttcaaca ctatgctcta tttagacata tgacagtttt tgaaaatata
     1081 gcttttggac ttagagtaaa acctaaaaaa gaaagagtta acaacaaaga gatagaagaa
     1141 aaagttacaa aacttttaaa actaatccaa cttgatggat ttgcttcaag atttccttgg
     1201 caactttcag gtggtcagag acaaagagtt gctttggcaa gagctttggc agttgagcca
     1261 aaagttttat tacttgatga gccttttggt gcgcttgatg caaaggtgcg aactgattta
     1321 agaagatggt taaaagagct tcaagaggag cttggaatta caactatttt agtaacacat
     1381 gatcaagagg aagcacttga agttgcaaat agaattgttg taataaacaa aggaaaaatt
     1441 gagcaaatag gaactcctga agatgtattt cataatccaa aaaatgagtt tgtaataaac
     1501 tttttaggaa atgtaaatct atttcacgca agagagattg aagatggagc taatttagaa
     1561 gatataacag ataataagta tcttattcgt cctcatgagt tagaaattgt agatataaat
     1621 gatagcgaag ctataattca tgcaaaagta aaatatatac aactagcagg gtcttttgta
     1681 aaagttgaac ttacatattt aaatgatgaa aaaagagttg ttttagttga gttggcacac
     1741 tatgaattta gtgataaaaa tataaaaaaa ggtg
//
LOCUS       DAOQAN010000265         1769 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_102188_length_1769_cov_2.633022, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000265 DAOQAN010000000
VERSION     DAOQAN010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102188_length_1769_cov_2.633022"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="PKO10_04755"
     CDS             complement(<1..375)
                     /locus_tag="PKO10_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNW66487.1"
                     /translation="MTNSQNRTIWILILSSSLILAITMGVRQSLGLFIAPINSSTSLD
                     IISISLALAIGQFVWGLTQPIFGAIADKKGSFGVLVFGALLMFFGLILTPFLTSEFSI
                     ILTLGLLVAAGAGAGSFSILIGA"
     gene            477..>1769
                     /locus_tag="PKO10_04760"
     CDS             477..>1769
                     /locus_tag="PKO10_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HNW66488.1"
                     /translation="MSKQLLQKQADTIRFLAADMVQRANSGHPGAPMGLADIATVLSK
                     HLNINPADEKWLNRDRLVFSGGHATGLVYSLLHLWGFDVSVNDMKNFRQSGSKTPGHP
                     EYGHTHGIEITTGPLGQGIANAVGFAMAGKYAQNLLGKDVINHKVYCLCGDGDLQEGI
                     SYEATATAGHLKLDNLVIIYDSNSITIEGDTSIAWSENVKKRFQAIDFEVIEIDGHNF
                     DQIDKAFVQAKNSTMPVLIIAKTVIAKGAVTLEGSHHSHGAPLGVDEIKQAKIKAGFN
                     PDVDFEVSADVKGAFDKLIIGSTMQNSWNESLSKETKEKISELQNPDFDSIVYPTFEA
                     GSSVATRDSNHKILNAIASKIPSFLGGSADLAPSNKTELKDMGDFPNGRNIHFGIKEH
                     AMAAIVNAMHLYGLFRVYSATFFVFSDYLKPSARIAALA"
BASE COUNT          661 a          249 c          320 g          539 t
ORIGIN      
        1 tgctcctatt aatatggaaa aacttcctgc ccctgcacct gctgctacaa gaagacctaa
       61 agttagaata attgaaaatt ctgaagttaa aaaaggagtt aatattaaac caaaaaacat
      121 caacaaagca ccaaagacta aaacaccaaa agagcctttt ttatctgcaa ttgcaccaaa
      181 aattggttga gttaatcccc aaacaaattg tccaattgca agagctaaac taatagagat
      241 aatatcaaga gaagttgaag agtttatagg agctataaac agacccaaag attgtctaac
      301 ccccattgtg attgctaaaa taagtgatga agagaggatt aaaatccaaa tagttctgtt
      361 ttgtgaattt gtcattttat aatctttttt ttgtaatttt agattaaaaa gtcttaaaca
      421 tagtttcgat ataatcttta caaattttta aaatcactca taatatagga taatctatgt
      481 caaaacaatt acttcaaaaa caagcagata caataagatt tttagctgct gatatggtac
      541 aaagagctaa ctcaggacat cctggagctc ctatgggact tgctgatatt gcaacagttt
      601 taagcaagca tttaaatata aatccagctg atgaaaaatg gctaaataga gatagattag
      661 tattttcagg tggtcatgca actggtttgg tttactcttt acttcattta tggggatttg
      721 atgtatcagt aaatgatatg aaaaacttta gacaatctgg ttcaaaaact ccaggacatc
      781 cagaatatgg gcatactcat ggaatagaaa ttacaacagg accactagga caaggaattg
      841 caaatgcagt tggatttgca atggcaggaa aatatgctca aaatttattg ggaaaagatg
      901 taataaatca taaagtttac tgtttatgtg gagatggaga tttacaagag ggaatctctt
      961 atgaggcaac tgcaactgca ggtcatctaa aacttgacaa tcttgtaata atttatgata
     1021 gtaattcaat cacgattgaa ggtgatacaa gtattgcttg gagtgaaaat gttaaaaaaa
     1081 gatttcaagc tattgatttt gaagtaatag agattgatgg acacaatttt gatcaaattg
     1141 ataaggcttt tgttcaagca aaaaattcaa caatgcctgt tttaattatt gctaaaacag
     1201 ttattgcaaa aggagctgtt actcttgaag gaagtcacca ttctcacgga gcacctcttg
     1261 gagttgatga gataaaacaa gcaaaaataa aagctggatt taatcctgat gttgattttg
     1321 aagtaagcgc agatgtaaaa ggtgcttttg ataaattaat tattggttca actatgcaaa
     1381 atagttggaa tgaaagttta tctaaagaga caaaagaaaa aatatctgag ttacaaaatc
     1441 cagattttga cagcattgtt tatccaactt ttgaggctgg aagtagtgtg gctacaagag
     1501 attcaaatca taaaatatta aatgctattg cttcaaaaat tcctagtttc ttaggtggaa
     1561 gtgcggattt agcaccatca aataaaacag aactaaaaga tatgggagat tttccaaatg
     1621 gaagaaatat ccattttggt attaaagaac atgcaatggc agctattgta aatgctatgc
     1681 acctatatgg tttatttaga gtttacagtg caacattttt tgtatttagc gattatttaa
     1741 aacctagtgc aagaattgct gcactggct
//
LOCUS       DAOQAN010000266         1764 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_102621_length_1764_cov_3.117613, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000266 DAOQAN010000000
VERSION     DAOQAN010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102621_length_1764_cov_3.117613"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="PKO10_04765"
     CDS             <1..699
                     /locus_tag="PKO10_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014469203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66489.1"
                     /translation="ITFPSPKVNYQLSYVKMIELFRGFDNIQSAVDSFKATDSSAEKK
                     AETAPLNICISCTYVNTSDARYCAFCGTNLIMGRGEKIFEVLKPLHNLDDKFQELLVV
                     SSKLHSVGSSLNFYKKNDNAFEFILNGLNYDFQHTSRVIVAHTIKFSKKSLPKQSDIE
                     EYKELLPNLETMQWLSFMIALNIAVNTDFSCPKVNYILDDDILRLKLPNRSFLINSYI
                     EKLELPKDLSLEIL"
     gene            696..1709
                     /gene="waaC"
                     /locus_tag="PKO10_04770"
     CDS             696..1709
                     /gene="waaC"
                     /locus_tag="PKO10_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HNW66490.1"
                     /translation="MVQKIAIIKLSAMGDIIHSMVALQFIKIKYPNIQIDWFVEDAFS
                     KVLENNPNINNIIKLNLKSIKNSKKEILTQLKLVKSFKNRDYDLIIDAQGLIKSAIVA
                     RLLGKNRVGFCKNSTREGVASFFYNKKVSISYDKNVIDRNCYLFSKALNFEISKDDIL
                     NKKPFLFFKNEDEKVYQYLDETRKNILLVVGASWKSKMYSKEKFVKIVQNLKENFFIA
                     WGNEEEKEIAEFICDNSDAIVLPKIDLNTLKALISRVDLVIGNDTGPTHMAWALNIPS
                     ITIFGNTPAYRNAYETKINKSVESSSIVNPFKLDKKDFSIKMVDENIIIDMAKELLSV
                     QKN"
BASE COUNT          670 a          209 c          270 g          615 t
ORIGIN      
        1 ataacattcc ccagccccaa ggttaattat cagttgtctt atgtgaaaat gattgagcta
       61 tttcgcggtt tcgataatat acagagcgct gtggacagtt ttaaggcgac agattcatct
      121 gccgaaaaaa aggcagaaac tgcgccgctt aatatttgta tttcatgcac ctatgtcaac
      181 acctcagatg cgcgttattg cgccttctgt ggaactaatc ttattatggg aagaggagaa
      241 aagatatttg aagttttaaa acctcttcac aatcttgatg ataagtttca agaactttta
      301 gttgtatctt ctaagcttca ctcagttggt tctagtttga atttttacaa aaaaaatgac
      361 aatgcttttg agtttatttt aaatggttta aattatgatt ttcagcatac ttcaagagtt
      421 atagttgctc atacaataaa gttttcaaaa aaatctttac caaagcagag tgatatagaa
      481 gagtataaag agttattacc aaatttggaa actatgcagt ggctttcatt tatgattgct
      541 ctaaatattg ctgtaaatac tgatttttct tgtccaaaag taaactatat tttagatgat
      601 gatattttac gattaaagct tccaaataga tcatttttaa taaattcata tattgaaaaa
      661 ctagagcttc caaaagattt atccttagag attttatagt gcaaaaaata gctattataa
      721 agctatctgc tatgggtgat attattcact caatggttgc tttacagttt ataaaaataa
      781 aatatccaaa tatccaaatt gattggtttg tcgaagatgc tttttctaaa gttttagaaa
      841 ataatccaaa tataaacaat ataatcaaac taaatttaaa atctattaaa aactcaaaaa
      901 aagagatttt aactcaatta aaacttgtaa aaagctttaa aaatagagat tatgatttaa
      961 tcatagatgc tcaaggttta ataaaatcag caatagttgc tagactgtta ggtaaaaaca
     1021 gagttggttt ttgtaaaaac tcaacaagag aaggagtggc tagttttttt tataataaaa
     1081 aagtatcaat atcttatgat aaaaatgtta tagatagaaa ttgctattta ttttcaaaag
     1141 cacttaattt tgaaatttca aaagatgata ttctaaataa aaaacctttt ttatttttca
     1201 aaaatgaaga tgaaaaagtt tatcaatatt tagatgaaac tagaaaaaat attcttcttg
     1261 tagttggtgc tagctggaag agtaaaatgt attctaaaga gaagtttgtt aaaatagtac
     1321 aaaatttaaa agagaatttt tttattgctt ggggaaatga agaagaaaaa gagatagcag
     1381 agtttatttg tgataattca gatgctattg ttttaccaaa gatagattta aatacattaa
     1441 aagcattgat aagcagagtt gatttagtaa ttggaaatga tacaggaccc acgcatatgg
     1501 cttgggcttt aaatatccca tcaataacaa tttttggaaa tactccagct tatagaaacg
     1561 cttatgaaac aaaaattaat aaaagtgtag agtcttcttc tattgtaaat ccatttaagt
     1621 tagataaaaa agatttttca atcaagatgg ttgatgaaaa tattattata gatatggcaa
     1681 aggagctttt aagtgtacag aaaaattaaa gattatttta gactcttttt atataacaca
     1741 tttctttttt tatttttaat aact
//
LOCUS       DAOQAN010000267         1764 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_102631_length_1764_cov_2.976594, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000267 DAOQAN010000000
VERSION     DAOQAN010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102631_length_1764_cov_2.976594"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..865)
                     /locus_tag="PKO10_04775"
     CDS             complement(<1..865)
                     /locus_tag="PKO10_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family peptidase"
                     /protein_id="HNW66491.1"
                     /translation="MDKKSGLLYISISVMVVVVIVLGVILTLDREEPNIYVQTEVNQK
                     DIYWNLQTPIRVEVADKSKLKSFETILIADQKEYVLENELVNEDQNSGILTFEIKPFK
                     ATDGFKATNAILRVKAKDSSSWNFFDGNEAIKDTNLIIDRRSPQASVISNSYMIKQGG
                     SGILIVEINDENLKDYYVTFNDEVIFELFPFHKKNFYISIITWPIDLTEFKGVKVVAI
                     DMAGNKASAKVPFYYDRFKEKVDNLNISDDFIYSVSKNVLELSNMEVPTNPAEIFVKA
                     NKDLRAKNLATM"
     gene            complement(858..>1764)
                     /locus_tag="PKO10_04780"
     CDS             complement(858..>1764)
                     /locus_tag="PKO10_04780"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014475260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HNW66492.1"
                     /translation="YILIITHVNPDPDSIGSALALSNLFSENKIKHKVFNISSDLPKN
                     LDFLNRFDKITNILPPFYDLAICVDCGTYKRLGFDLPKDVPLINFDHHASNNNFGTIN
                     IVDPYKSSTAEIVFEFFKHNGLNISKYSAQSLYCGIYDDTLAFSLGRCDEETFKKVNF
                     LVECGASPSFIANKLLRRDSLAKYRIIPKVLDSLELHKDGEIATIYALEEWFKTTGAH
                     ARDCEDALDMIMSMQIVQVAFFIRFTNGACRVSLRSKNELDVSKIASIFDGGGHFNAS
                     GCTIDTKDVEVAKNLVLKEVIKYYG"
BASE COUNT          625 a          302 c          176 g          661 t
ORIGIN      
        1 tcatagttgc tagatttttt gctcttaaat ctttatttgc ttttacaaag atctcagcag
       61 gatttgtagg aacctccata ttactaagtt ctaaaacatt tttactaaca ctataaataa
      121 aatcatcact aatatttaaa ttatcaactt tttctttaaa tctatcataa taaaaaggaa
      181 ctttagcaga agctttgttt cctgccatat caattgcaac aaccttaaca cctttaaact
      241 ctgttaaatc tattggccat gtaataatag agatataaaa attctttttg tgaaatggga
      301 aaagttcaaa aataacttca tcattaaatg taacataata atcttttaaa ttttcatcat
      361 ttatttcaac aattaaaatt ccactaccac cttgttttat catatatgaa tttgaaataa
      421 cactagcttg tggagatctt ctatcaatta ttaaatttgt atctttaata gcttcatttc
      481 catcaaaaaa attccaacta ctactatctt tagcttttac tcttaaaatt gcatttgtag
      541 ctttaaaacc atctgttgct ttaaaaggtt ttatttcaaa agtcaaaatt ccactatttt
      601 gatcttcatt tactaactca ttttctaaaa catactcttt ttgatcagct attaagattg
      661 tttcaaaaga ttttaattta cttttatctg caacttcaac tcttattgga gtttgtaaat
      721 tccaataaat atctttttga ttaacttctg tttgaacata aatatttggt tcttctctat
      781 ccagagttaa aataacacct agaacaataa ccacaacaac cataactgaa atacttatat
      841 ataataatcc acttttttta tccataatat tttataactt cctttaatac taaatttttt
      901 gcaacttcaa catcttttgt atctattgta catcctgatg cattaaaatg cccaccacca
      961 tcaaaaatag aagctatttt tgagacatca agttcatttt tagacctcaa agatactcta
     1021 caagctccat ttgtaaatcg tataaaaaat gcgacttgta ctatttgcat actcataatc
     1081 atatcaagag catcttcaca atctctagcg tgtgcaccag tagttttaaa ccactcttca
     1141 agagcataaa tagtagctat ttctccatct ttatgaagct ctaaactatc taaaactttt
     1201 ggaatgattc tgtattttgc caaactatct cttcttaaaa gcttatttgc aataaatgat
     1261 ggactagcac cacactcaac caaaaagtta acttttttaa aagtctcttc atcacatctt
     1321 cctaaagaaa atgctaaggt atcatcatat ataccacaat aaagtgactg cgcactatat
     1381 tttgatatgt ttaatccatt atgtttaaaa aactcaaaaa caatctctgc tgtactactt
     1441 ttatatggat ctactatatt tattgtgcca aaattattat ttgaagcatg atggtcaaaa
     1501 tttataagag gtacatcttt tggtaaatca aatccaagcc ttttgtaagt tccacaatca
     1561 acacaaattg ctaagtcata aaaaggtggt aaaatattcg taattttatc aaatctattt
     1621 aaaaaatcta aattttttgg taaatcagaa ctaatattaa aaactttgtg ttttattttg
     1681 ttctctgaaa ataaatttga taaagccaaa gcactgccta tagaatcagg atctggattt
     1741 acatgagtta ttattaaaat gtat
//
LOCUS       DAOQAN010000268         1762 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_102837_length_1762_cov_2.616872, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000268 DAOQAN010000000
VERSION     DAOQAN010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102837_length_1762_cov_2.616872"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1241)
                     /locus_tag="PKO10_04785"
     CDS             complement(<1..1241)
                     /locus_tag="PKO10_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66493.1"
                     /translation="MNKKRNIFLIILAFIVSTFFAIILTNYYINKKQKELLNSIYEST
                     HKNIFEKTQNLINDKQNTSLAIAISLSKDETLYTHLKNRDFEKLNYVQIAKLIETNSK
                     YKNIWIQILDSNLNSVYRSWTNIRGSLNFRPDLKKVEALKNISTSISAGLFNIGIRAR
                     TPIFDENNNFYGALEVVTHFDSITEDLVKNNISPVVIGDKSIRKNLTNPLLSKLFIDD
                     YYIANENVDMNLYNYIKENGVEKYINIDKFIIENGYLISKYPLFDENKKNLAYIINFM
                     KLNNINLENIKHIKIQTTLGTVIALIILFTAILVYYLRDIKYQNSKNKIILNSQPNIV
                     IITNGKEIIDANKQLLKFFPSVKDLEDFKKRYICICYTFEDLEKDDYIINKDYNGKNW
                     VEYIFENQDKNFKVAIKNSKE"
     gene            1351..>1762
                     /locus_tag="PKO10_04790"
     CDS             1351..>1762
                     /locus_tag="PKO10_04790"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012857362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HNW66494.1"
                     /translation="MIKRYPTKQIYVGNVPIGGDAPISVQSMTFTKTSDVEATVEQIK
                     KLHFAGADIVRVAVPHLEDAQALKEIKKQVDLPIVADIHFHYKLALIAAEVVDCIRIN
                     PGNIGDKKRVAEVVKACQARNIPIRIGVNCGSLEK"
BASE COUNT          615 a          207 c          213 g          727 t
ORIGIN      
        1 tcttcttttg aattttttat agcaacttta aagtttttat cttgattttc aaatatatat
       61 tcaacccaat ttttaccatt ataatcttta tttataatat aatcatcttt ttctaaatct
      121 tcaaaagtat aacaaataca aatatatctt tttttaaaat cttcaaggtc tttaaccgaa
      181 ggaaaaaatt ttaatagttg tttgtttgca tctattatct cttttccatt tgtaattatt
      241 acaatatttg gttgtgagtt taaaataatt ttatttttac tattttgata ttttatatct
      301 cttaaataat atactaaaat agccgtaaat agtataataa gtgcgataac tgttcctagg
      361 gtagtttgaa tttttatatg ttttatattt tcaagattta tattatttaa tttcataaaa
      421 tttattatat atgctaaatt ttttttattt tcatcaaaaa gtggatattt tgagattaaa
      481 taaccatttt ctattataaa tttatctata tttatatatt tttcaacgcc attttctttt
      541 atatagttgt ataaattcat atcaacattt tcatttgcaa tatagtaatc atctatgaaa
      601 agtttagata aaagaggatt tgtcaaattt tttctaatac ttttatctcc tattacaaca
      661 ggagaaatat tgttttttac taaatcttca gtgatcgaat caaaatgtgt aacaacctct
      721 aatgctccat aaaaattatt gttttcatca aaaataggag ttcttgctct tattcctatg
      781 ttaaaaagac cagcactaat agaggttgaa atatttttca aagcttctac tttttttaag
      841 tcaggtctaa agttaagact acctcttata tttgtccatg atctataaac actatttaag
      901 ttactatcaa gaatttgaat ccagatgttt ttatactttg aatttgtctc tattaatttt
      961 gcaatttgta catagtttag tttttcaaaa tctctgtttt ttagatgtgt atataaagtt
     1021 tcatcttttg ataatgatat tgcaatagcc aaagatgtgt tttgcttatc gtttattaga
     1081 ttttgtgttt tttcaaaaat atttttatgt gtagattcat atattgagtt tagtagttct
     1141 ttttgttttt tatttatata ataatttgtt aatattattg caaaaaaagt tgaaacaata
     1201 aatgcaagga taattaaaaa tatatttctt tttttgttca aaactatttt cttcttctct
     1261 tttaaatcta agttacaagg atataaaata atttcttatt tataaattaa acttcacttt
     1321 tatttaaact attttagata aaatcccaaa atgataaaaa gatacccaac aaaacaaata
     1381 tatgtaggaa atgttcctat tggtggtgat gcaccaatta gtgttcagtc aatgacattt
     1441 acaaaaacat cagatgtaga agcaacagtt gagcaaataa aaaaacttca ttttgcagga
     1501 gctgatattg taagagttgc tgttcctcat cttgaggatg ctcaagcttt aaaagagata
     1561 aaaaaacaag ttgatttacc aatcgttgct gatattcact ttcactataa gttagcttta
     1621 attgcagctg aagtggttga ttgtataaga atcaatccag gaaatattgg agataaaaag
     1681 agagtagcag aagtcgtaaa agcttgtcaa gctagaaata ttccaatacg aattggagta
     1741 aactgtggaa gtttagaaaa ag
//
LOCUS       DAOQAN010000269         1762 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_102858_length_1762_cov_2.137668, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000269 DAOQAN010000000
VERSION     DAOQAN010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102858_length_1762_cov_2.137668"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="PKO10_04795"
     CDS             <1..576
                     /locus_tag="PKO10_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HNW66495.1"
                     /translation="SNKLISSTNSMLLIGIALSYFYSSALMLIFFMSNLQENYSIVRF
                     TLGSLDTVGFLPSFVVSVVAICLIIFIYKMKSSINLLLISNNMAFLKGLNVNKTILIL
                     LLFITICVGICISFTGPIGFVGLVIPHIVKLIYKQSATKLFIPTLFFGGTFLVFCDLI
                     SRVLPTASSLPIGVVTAFIGAPFFVYLLIRK"
     gene            complement(587..1084)
                     /locus_tag="PKO10_04800"
     CDS             complement(587..1084)
                     /locus_tag="PKO10_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014474180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNW66496.1"
                     /translation="MKELILIRHAKSSWSNPLLEDFERPLNKRGAKNAPFMAKVLKQK
                     EVNPDLIISSPSKRTKDTLDFFIKEFDYKGEIIFEESIYEAPYINILKVIKNIDDKHK
                     TIFLFGHNPGFNDLADFLLGRFEDNIPTCGVLKLNFNISKWEDIKEKIGILEFFIYPK
                     MFDKS"
     gene            1167..>1762
                     /locus_tag="PKO10_04805"
     CDS             1167..>1762
                     /locus_tag="PKO10_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like cysteine peptidase"
                     /protein_id="HNW66497.1"
                     /translation="MKILISIFFLLFFTINADFVDTSLILKVEEKYGKFAKNRFIALN
                     TMLEKSKNSDLRTKLEKINDFFNDIKYASDQTVYGTSDYWANPYEFLAKDKGDCEDYA
                     IAKYLALEYLGVPTSKMFLSYVRVKSSNEAHMVLTYFETPSSEPLVLDNLRKTIQPAS
                     KRDDLIPVFNFNPNILKGEKTAAHRKWDTLIKDYKGKK"
BASE COUNT          612 a          219 c          223 g          708 t
ORIGIN      
        1 tctaataaac ttataagttc gacaaattcg atgcttctaa taggaattgc tctatcgtat
       61 ttttatagtt cagcacttat gcttatattt tttatgagta atcttcaaga aaactattca
      121 atagtaagat ttactttggg aagtcttgat acagttggat ttcttccttc ttttgttgta
      181 tctgttgtgg caatttgtct tattattttt atttataaaa tgaaaagtag tataaatcta
      241 cttcttatat caaataatat ggctttttta aaaggtttaa atgtaaataa aactattttg
      301 attttacttc tttttattac tatttgtgtt ggtatttgta ttagttttac aggacctatt
      361 ggctttgtag ggcttgtaat tcctcatatt gtaaaactaa tatataagca aagtgcaact
      421 aaactattta taccaacact attttttggt ggaacttttt tagttttttg tgatttaatt
      481 tcaagagttt taccaacagc ttcaagctta ccaataggtg ttgtaactgc ttttattggg
      541 gctccatttt ttgtatatct tttaatacgt aaataaatct ttttctttaa gatttatcaa
      601 acatttttgg atatataaaa aactctaaaa ttccaatttt ttctttaata tcttcccatt
      661 ttgatatatt aaaatttagt tttaatactc cacatgtagg gatattatct tcaaatcttc
      721 ctaataaaaa atctgctaaa tcattaaatc caggattatg tccaaataaa aatatagttt
      781 tatgtttatc atctatattt tttataactt ttaaaatatt aatataaggt gcttcataaa
      841 tcgattcttc aaagataatt tctcctttat aatcaaactc tttgataaaa aaatctaaag
      901 tatcttttgt tctttttgat ggtgaagaga ttattaaatc tggatttacc tctttttgtt
      961 ttaaaacttt tgccataaaa ggtgcatttt ttgcgcccct tttatttaaa ggtctttcaa
     1021 aatcttctaa aagaggattt gaccaactag attttgcatg tcttattaaa atcaactctt
     1081 tcatataaaa tccttcaatt tttttctcat tttatcatat attatagaaa agaaaaaaat
     1141 atattatttt tagatataat aattacatga aaattttaat tagtatattt tttctactgt
     1201 tttttactat aaatgctgat tttgttgata cctctttaat tttaaaagtt gaagaaaaat
     1261 atggaaaatt tgcaaaaaat aggtttattg cattaaatac tatgttggaa aagagcaaaa
     1321 actctgattt aagaacaaag cttgagaaga taaatgattt tttcaacgat ataaaatatg
     1381 caagtgatca aactgtttat ggtactagtg attattgggc aaacccttat gaattcttag
     1441 caaaagataa aggagattgt gaagattatg caattgcaaa atatcttgca cttgaatact
     1501 taggagttcc aacttctaaa atgtttttat cttatgttag agttaaatct tcaaatgaag
     1561 cacatatggt tttgacatat tttgaaacac caagttctga acccttagtt ttagataatt
     1621 taagaaaaac tatacagcca gcttcaaaaa gagatgattt gataccagtt ttcaacttta
     1681 atccaaatat tctaaagggt gaaaaaacag ctgctcacag aaagtgggat acattaatta
     1741 aagattataa aggaaaaaaa at
//
LOCUS       DAOQAN010000270         1756 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_103422_length_1756_cov_2.033510, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000270 DAOQAN010000000
VERSION     DAOQAN010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103422_length_1756_cov_2.033510"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..880)
                     /locus_tag="PKO10_04810"
     CDS             complement(<1..880)
                     /locus_tag="PKO10_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66498.1"
                     /translation="MGLKNLKVIYKINIISLITIILLGIVTFVLYNGMSKINNSFENS
                     SSISDLALTITKTSEQGLQVSNALRGIIVNPDDTKAKENFIQAVKELDDLMLVLKDST
                     KKSQGFEKFEIAPLYASQIKVLNKIIEKIKNSEALTKEDNTLSTKEWRPLKAALLKWQ
                     EKNLERNKEIKEELNKTVSSVTTFILIILLITILSILVTIQLITRNIVSSLNIFQNGL
                     LSFFAFSNKESSNVQLINLDGKDEFGQMAKVINQNIQKTQDLINQDNALIEDVKRVVN
                     EVKAGHLYAKIEKSTIN"
     gene            complement(993..1490)
                     /locus_tag="PKO10_04815"
     CDS             complement(993..1490)
                     /locus_tag="PKO10_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014474474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4065 domain-containing protein"
                     /protein_id="HNW66499.1"
                     /translation="MNIDMTKIANIILYMLHKQVKALNHKKIELLIFFCELNHLNFCG
                     KKILGETFIKTSRGVKAEVLDELFTLILDEVEFEDEEDDRVFFIQELMDFLEIEIVEK
                     ERFKELKFAKLDEDFDETIFTSDELKSIHKVINLYKDTSVRNLSNECFSLEKVRKSEN
                     GAIVL"
     gene            complement(1499..>1756)
                     /locus_tag="PKO10_04820"
     CDS             complement(1499..>1756)
                     /locus_tag="PKO10_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HNW66500.1"
                     /translation="YNNFDFKNFNNFNNQNFQGNFYNKGSKLQEAKEFFGFSATPSKD
                     DIKKRFKELAKIYHPDLNGGDEEKMKKLNEYRDILMQAYGQ"
BASE COUNT          574 a          253 c          170 g          759 t
ORIGIN      
        1 catttatagt tgatttttct atttttgcat ataaatgacc agcttttacc tcatttacaa
       61 ctcttttaac atcttcaatt agagcattgt cttgatttat taaatcttgt gttttttgga
      121 tattttggtt tattactttt gccatttgac caaattcatc ttttccatca agatttatta
      181 attgaacatt tgaactctct ttgtttgaaa aagcaaaaaa acttaaaaga ccattttgga
      241 aaatattaag actagataca atatttctag taattagctg aattgttact aatatactta
      301 aaatagttat aagtaaaata attaatataa aagttgtaac acttgatact gttttattta
      361 actcttcttt aatctcttta tttctttcaa gatttttctc ttgccatttt agtaaagcag
      421 cttttagtgg tctccactct tttgttgaaa gagtattatc ctcttttgtc aaagcttcac
      481 tgttttttat tttttcaatt attttattta ataccttgat ttgagaagca tataaaggag
      541 caatttcaaa tttttcaaaa ccttgagatt tctttgtgct atcttttaaa actaacatta
      601 aatcatcaag ctcttttact gcttgtataa agttctcttt tgcttttgta tcatctggat
      661 ttacaattat tcctcttaaa gcatttgaaa cttgaagtcc ttgttcgcta gtttttgtaa
      721 tagtaagagc taagtcagat atagaactac tattttcaaa actattgttg attttagaca
      781 taccattgta taaaacaaat gttacaatac caagtaaaat tattgtaatt aaagatatta
      841 tatttatctt atagattact tttaaatttt tcagacccat ttttatcccc tttagagtat
      901 taagtagata aaaatagtat catataatct atgatattat tcttaggttg aattaatttc
      961 gctaaataat aaaagattat agttttattt ttttataaaa ctattgctcc attttcactt
     1021 tttcttactt tttctaaact aaaacattca ttagaaaggt ttcttacgct tgtatcttta
     1081 tataaattta taactttatg aatagatttt aactcatcag atgtaaaaat agtttcatca
     1141 aaatcttcat ctagttttgc aaatttaagc tctttaaatc tctctttttc aactatttca
     1201 atctctaaaa aatccattaa ctcttgaata aaaaatactc tatcatcctc ttcatcttca
     1261 aactctactt catctaaaat tagtgtaaaa agttcatcta aaacttcagc tttaacacct
     1321 ctagaagttt ttataaatgt ttcacccaaa atcttttttc cacaaaagtt taagtgattt
     1381 aattcacaaa aaaatattaa aagctctatt tttttgtgat ttaaagcttt aacttgtttg
     1441 tgtagcatat ataatataat atttgctatt tttgtcatat ctatattcat aaaaatcctt
     1501 attgcccgta agcttgcatt aaaatatctc tatattcatt taattttttc attttttctt
     1561 catctccacc atttaaatca ggatgataaa tttttgctaa ctctttaaat ctctttttta
     1621 tatcatcttt tgatggagtt gctgaaaaac caaaaaactc ttttgcttct tgaagttttg
     1681 aacctttatt atagaagttt ccttgaaaat tttggttatt aaagttattg aagtttttaa
     1741 aatcaaaatt attgta
//
LOCUS       DAOQAN010000271         1751 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_103886_length_1751_cov_1.781250, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000271 DAOQAN010000000
VERSION     DAOQAN010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103886_length_1751_cov_1.781250"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="PKO10_04825"
     CDS             <1..433
                     /locus_tag="PKO10_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014469272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HNW66501.1"
                     /translation="LTIIEAIADIAEFTKAPEILLEHVRAKLFRLITQKYKDTDGVLH
                     IVTIKPELEQQFIGKLQDHHGASQLMLSIAEINNLVTKTKQLLDDIEKKGFSKVAMVV
                     DPVLRKRVSEIYEKFGLSIPVLSHAELDSKANFAIEGTLEF"
     gene            461..1645
                     /locus_tag="PKO10_04830"
     CDS             461..1645
                     /locus_tag="PKO10_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNW66502.1"
                     /translation="MKYYINFLRDKPIIRSLSLVNFIASFGAWFSTVAIYTMVVEFGS
                     SEFAIAIVTAMHFIPAILIAPFSGSLIDRLKIKPLMVTLLFIELSMTACFLLIETSSQ
                     LWLLLIFIFIRMSAASMFFSSEMSLLAKIATGKDLQTANEIQSIIWSFTYAVGMAVSG
                     FIVNLYGVKAAILIDVAIFVLAFFVFLNIKINIEYKKVEEKILELMIDGLRYIKNNKI
                     ILHLIFLHASVGLTSYDALITILAKNEYKEIIAVPLAIGLSNAVRAVALMIGPIFLNK
                     IIKKDNLQYLLVFQGITIIIWAFAQSDFYLSLGALFFVGFSTAFLWSYTYALLQNHCD
                     KRYIGRVISYNDMFFMLANVLTTLFIGTMAHITTTSIITICLGVAFLLYAYYYTKIYS
                     KI"
BASE COUNT          586 a          205 c          283 g          677 t
ORIGIN      
        1 gctcacaata attgaggcaa tagcagatat tgctgagttt acaaaagctc cagagatact
       61 cttagagcat gtaagagcaa aactttttag acttattact caaaaatata aagatacaga
      121 tggagtttta catatagtta ctattaaacc agagcttgaa caacagttta taggaaaact
      181 tcaagatcat catggtgcaa gtcagttgat gttaagtatt gcagagataa ataatcttgt
      241 aacaaaaaca aaacaacttt tagatgatat tgagaaaaaa ggtttttcaa aagttgcaat
      301 ggttgttgat cctgttttaa gaaaaagagt atcagaaatt tatgagaagt ttggtctttc
      361 tattcctgtt ttatctcacg cagagcttga ttcaaaagca aattttgcta ttgaaggaac
      421 tttagagttc tgattttttt tcattttttt aaggattttt ttgaaatatt atattaattt
      481 tttaagagat aaacctatta ttagaagttt atctttggtg aactttattg catcttttgg
      541 tgcatggttt tctactgttg caatttatac tatggttgta gagtttggtt caagtgaatt
      601 tgctattgca attgttacag ctatgcattt tattccagca atcttaatag ccccatttag
      661 tggaagttta atagatagat taaaaataaa accacttatg gtaactttac tttttataga
      721 gttatctatg actgcttgtt ttttgttaat agaaacatcc tcacaacttt ggttacttct
      781 tatttttatt tttatacgaa tgagtgctgc ttcaatgttt ttttcaagtg aaatgtcact
      841 tttagcaaaa attgctaccg gaaaagatct tcaaacagca aatgagatac aatcaattat
      901 ttggtcattt acttatgctg ttggaatggc agttagtggt tttatagtaa atttatatgg
      961 tgtaaaagct gctattttga tagatgttgc tatttttgta ctcgcttttt ttgtttttct
     1021 taatattaaa ataaatatag aatacaaaaa agttgaagag aaaattttgg aacttatgat
     1081 tgatggttta agatatatta aaaataataa aattatttta catctgattt ttttacatgc
     1141 aagtgtaggg cttactagtt atgatgcttt gattactatt ttggcaaaaa atgagtataa
     1201 agagattata gctgtccctc ttgctattgg gctttcaaat gctgtaagag cggttgctct
     1261 tatgattggt ccaatatttt taaataagat tatcaaaaaa gataatcttc aatatctttt
     1321 agtttttcaa ggaattacaa taataatttg ggcatttgct caaagtgatt tttatttatc
     1381 tttaggtgct ctgttttttg taggatttag tacagcattt ttgtggtctt acacttatgc
     1441 acttttacaa aatcattgcg ataaaagata tataggaaga gtaatatcat ataatgatat
     1501 gttttttatg ctagcaaatg ttctaacaac tctgtttatt ggaactatgg cacatattac
     1561 aacaacttca attattacga tatgtttagg agttgcattt ttactttatg cttactacta
     1621 tacaaagatt tattcaaaaa tttaattgta taatgattta aaaggagaaa attatgattt
     1681 tagactacaa agatgtaaat gatttagata gatacaaaat aatgtcagga agtatagttc
     1741 cacgaccaat t
//
LOCUS       DAOQAN010000272         1750 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_103949_length_1750_cov_2.520944, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000272 DAOQAN010000000
VERSION     DAOQAN010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103949_length_1750_cov_2.520944"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(152..>1750)
                     /locus_tag="PKO10_04835"
     CDS             complement(152..>1750)
                     /locus_tag="PKO10_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014475417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNW66503.1"
                     /translation="LSTIDILGQFYSIEKYEHLEKLSLIKSLLEYGWLIQVAFDQVKL
                     NEASKLELINSSVALSSAYLKMLESGNSEFVLPEIKNYSDHLEYLQDQFLKIDITQQL
                     NIVKRNFDINSPSSNRLKNKLLLVENRIKDRINATTNKILIEEFFKEQGLDEQEKILF
                     LALLKEEYSGGDGTLREMNYLLELISNDDYEKIKYRSLLEESSTLISKNLVDYDEVLT
                     PFGGINRNFYIPDEVLYKISHPNKKGSTVSKIKLETIIKDQEMFELISAQKSLEDVVL
                     NPKTKETLNNLLKQVDKSVINRLKAWGIKDKKRGIDAKIIFYGVAGTGKTITALALAK
                     SLKKEVLSFDCSKILSMYIGESEKNVRNIFDKYNEIKEQTKTEPILLLNEADQFLSSR
                     SSGTGSSADKMHNQMQNIFLEQIERFDGILIATTNLLENLDKAFSRRFNYKIEFVKPN
                     HEQRVELWKKLIPATLPLENGFDFEKIAKHELTGGQIELVIKNTAYKLAVLEDAVFTL
                     KDFEEQISKEKKGQFDSETKVGFF"
BASE COUNT          547 a          294 c          168 g          741 t
ORIGIN      
        1 aattcaaaat tattaattgg gttcatatat ataaaagtag cactattttt aatagctttt
       61 tttaaactat ttctaaactc ctcatcttca aaaaatgccc caacacttaa aatataatca
      121 taattttcta taattttcac taatcttcct tttaaaaaaa tccaactttt gtttcactat
      181 caaattgacc ttttttctct ttacttattt gctcttcaaa atcttttaga gtaaaaactg
      241 catcttctaa aacagctagt ttataagctg tatttttaat aaccaactct atttgaccac
      301 ctgtaagctc atgttttgca attttttcaa aatcaaaacc attttctagt ggtaaagttg
      361 ctggaatgag ttttttccaa agctctactc tttgctcatg atttggtttt acaaactcaa
      421 ttttatagtt aaatcttctt gaaaatgctt tatcaagatt ttcaagcaaa tttgtagttg
      481 ctattaaaat tccatcaaat ctctcaattt gctccaaaaa tatattttgc atttgattat
      541 gcatcttatc agcactactt ccagttccac tacttcttga gcttaaaaat tggtctgctt
      601 catttaaaag taaaattggt tctgtttttg tttgctcttt tatctcatta tatttatcaa
      661 aaatatttcg tacatttttt tcactctcac ctatatacat tgatagaatt ttagagcaat
      721 caaaacttaa aacctctttt tttaatgact ttgcaagagc taaagctgtg attgtttttc
      781 cagttccagc aactccataa aaaattattt ttgcgtctat tcctcttttt ttatctttta
      841 ttccccaagc ttttagtcta tttataacac ttttatcaac ttgttttaaa agattattta
      901 aagtctcttt tgtttttgga tttaaaacaa catcttctaa acttttttgt gcagaaatta
      961 actcaaacat ctcttgatct tttataattg tttcaagttt tatttttgaa actgttgagc
     1021 cttttttatt tggatgagat attttatata aaacttcatc tggaatataa aagtttctat
     1081 ttattccacc aaatggtgtt aaaacctcat cataatcaac caaatttttt gaaatcaaag
     1141 ttgaactctc ttcaagaaga cttctatatt ttatcttctc ataatcatca tttgatatta
     1201 gctcaagtag atagttcatc tctcttagtg ttccatctcc tccactatac tcctctttta
     1261 aaagagccaa aaatagtatt ttctcttgct catccaagcc ttgttcttta aaaaattctt
     1321 ctattaaaat cttgtttgta gttgcattta ttctatcttt tattctattt tctacaagta
     1381 aaagtttatt ttttaatcta tttgaactag gagaatttat atcaaaattt ctttttacta
     1441 tatttagttg ttgtgtgata tcaattttta aaaattggtc ttgaagatac tctaagtggt
     1501 cactataatt ttttatttca ggtaatacaa actcactatt tccactttca agcattttta
     1561 aatatgcact agaaagtgct acacttgagt ttataagttc taattttgaa gcttcattta
     1621 attttacttg atcaaaagca acttgaatca accatccata ctcaagcaaa gattttatca
     1681 aacttagttt ttctaaatgt tcatattttt caatactata aaactgtcct aaaatatcaa
     1741 ttgtacttag
//
LOCUS       DAOQAN010000273         1743 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_104582_length_1743_cov_2.880924, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000273 DAOQAN010000000
VERSION     DAOQAN010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104582_length_1743_cov_2.880924"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="PKO10_04840"
     CDS             <1..226
                     /locus_tag="PKO10_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66504.1"
                     /translation="AILFSLLSSAMVLEKILNKKFAPMIETVRYIDFGNIFINNSVVI
                     IILFIVIFISMFTYKYIEVKGQEFGKRFLK"
     gene            291..>1743
                     /locus_tag="PKO10_04845"
     CDS             291..>1743
                     /locus_tag="PKO10_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNW66505.1"
                     /translation="MIELINIQKSYPTQTLYQDLNLRLNKGDKVGLVGRNGTGKSTLF
                     KLILGEEQPDSGDIATPKNYKIGALKQYFDFKEKTLLDETATALSEDDKYNIYKAEKI
                     LFGLGFSEEDLQKEPKSFSGGYQIRINLAKLLLTEPNMLLLDEPTNYLDILSIRWLRE
                     FLKSFDGEVILITHDRDFMNSVCTHTMGIIRKNAFIIAGSTTKFFEQLAANEEHYMKQ
                     KEAQDKKIKDLEEFIAKNKARAATATLAQSKVKILEKMEVMEDIVYEKDLKFDFNYKD
                     TSAKYLLEVKDVSFGYDKSNILFKDITFALSRGETLGIIGKNGKGKSTLLNVLAGELK
                     TLSGSVDFHPSTVFGHFGQTNINHLNQNNTIIEEIQSVNNKISESVIRNICGIMMFSG
                     DNAKKKISLLSGGEKSRVMLGKIIAQDVNLLFLDEPTNHLDIESIEALTTAIKEFEGS
                     SIIVTHSEELLRAVCDRLIVFTNDGADYFNGTYD"
BASE COUNT          695 a          196 c          296 g          556 t
ORIGIN      
        1 tgcaattttg ttttctcttc tttctagtgc aatggtttta gaaaaaatac taaataagaa
       61 atttgcacca atgattgaaa ctgttagata tattgatttc ggaaatattt ttataaataa
      121 ttctgttgtc attatcatac tttttatagt aattttcata tctatgttta cttataaata
      181 tattgaagta aaagggcaag agtttgggaa aaggttttta aaataaccaa cctctaacac
      241 aaaatataat ataatcccgc cttaaaaatt taaaaaaata gagaaaacag atgatagagc
      301 taataaatat acaaaaatca tacccaacac aaactttata tcaagattta aatttaagat
      361 taaataaagg cgataaagtt ggacttgttg gacgaaatgg tacaggaaaa tcaacacttt
      421 ttaaacttat tcttggagaa gagcaacctg atagtggaga tattgcaact ccaaaaaact
      481 ataaaatagg tgctttgaaa caatattttg atttcaaaga gaaaacttta cttgatgaaa
      541 cagcaactgc tttaagtgaa gatgataagt acaatattta taaagctgag aaaatattgt
      601 ttggtttagg atttagtgaa gaagatttac aaaaagagcc aaagagtttt tcaggtggtt
      661 atcaaataag aataaattta gcaaaacttc ttttaacaga gccaaatatg ctacttcttg
      721 atgagcctac aaactattta gatattttat ctattagatg gttaagagag tttttgaaaa
      781 gttttgatgg agaagttata cttattactc acgatagaga ttttatgaat agtgtttgta
      841 cacacactat gggaattatt agaaaaaatg cttttataat tgctggaagt acaactaaat
      901 tctttgaaca attagcagca aatgaagagc attatatgaa gcaaaaagag gctcaagata
      961 aaaaaataaa agaccttgaa gagtttattg caaaaaataa agcaagggca gcaacagcaa
     1021 ctttggctca atcaaaagta aaaattcttg aaaaaatgga agttatggaa gatattgtct
     1081 atgaaaaaga tttgaagttt gatttcaatt acaaagatac aagcgcaaaa tatctgcttg
     1141 aagtaaaaga tgtaagcttt ggatatgata aaagtaatat tcttttcaaa gatataactt
     1201 ttgcattaag tcgtggtgaa actttaggaa ttataggaaa aaatggaaaa ggtaaatcta
     1261 cacttttaaa tgttcttgca ggtgaattaa agactcttag tggaagtgta gattttcatc
     1321 caagcacagt ttttggtcat tttggtcaaa caaatataaa tcatttaaat caaaataata
     1381 caattattga agagatacaa agtgtaaata ataaaatatc agaatcagtt atacgaaata
     1441 tttgtggaat tatgatgttt agtggagata atgcaaagaa aaagatatct cttctttcag
     1501 gtggagaaaa aagtagggta atgcttggaa aaattattgc acaagatgta aatcttcttt
     1561 tccttgatga gcctacaaat caccttgata ttgaatctat tgaagcactt acaactgcta
     1621 taaaagagtt cgagggttca agtattatag taactcacag tgaagagcta ctaagagcag
     1681 tttgtgatag attaatagtt tttacaaatg atggagctga ttatttcaat ggaacttatg
     1741 atg
//
LOCUS       DAOQAN010000274         1742 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_104688_length_1742_cov_2.321873, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000274 DAOQAN010000000
VERSION     DAOQAN010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104688_length_1742_cov_2.321873"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /locus_tag="PKO10_04850"
     CDS             complement(<1..499)
                     /locus_tag="PKO10_04850"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF21 domain-containing protein"
                     /protein_id="HNW66506.1"
                     /translation="MTLLVTYLLLTLILSFMCSLLEATLLSSTSSYIESLDKKGYSPK
                     TVDLAKDVKQNIDKSISSILTLNTFANTMGAAGVGAQAAIIFGSNWQAVIAFILTLMV
                     LFISEIFPKTLGAIYWRKFIVPAVYIISFMVKITYPFIFIATFITNTLQKGRKNEVNF
                     SKDEII"
     gene            complement(555..>1742)
                     /locus_tag="PKO10_04855"
     CDS             complement(555..>1742)
                     /locus_tag="PKO10_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66507.1"
                     /translation="ISIKNSFFTLYPLIKSGLYQEIFDIIIDIMKKREDLQFKITNKI
                     KNLEIGELKDNLFHYSYDSKTLKSLFKNNISKDNISYITAHSSFKGEIYENIIYELLM
                     DYALNNDDIKGFVLKGPYQDMENKFIKSGLLIDRTSQIVFKSAYKDISEFDAMFFTEN
                     KLYFVEMSTSKKTSSLNKRLAKKYALLKMIFPSLEINALIVLTAGSVGLNNFPSYATI
                     WVTKDLDDDDLIEKIIFAKKVKNDLQTLKAPENKKYLEAFSLKYKKFAYFPTLEWILN
                     GARKNPKFKIDLSFFSNSKMSLYFDIYTKLYIGYLNIDCFKEFYKDFEMELESNRVFV
                     TLEKVTQTQIDIVYYAKLKNKKLYRIRLEDGQTPSIKEKEPDGFTNAEVRFFLKFLKK
                     NIF"
BASE COUNT          634 a          233 c          175 g          700 t
ORIGIN      
        1 taataatctc atcttttgaa aagttcactt cattttttct acctttttgc aaagtgtttg
       61 ttataaaagt agcaataaaa ataaatgggt aagtaatttt aaccataaac gatattatat
      121 aaacagctgg aactataaac tttctccaat aaatagcacc aagagttttt ggaaatatct
      181 cagatataaa aagtaccatt aaagttaaaa taaaagcaat cacagcttgc caatttgaac
      241 caaaaataat agcagcttgt gcaccaacac cagctgctcc catagtattt gcaaaagtgt
      301 ttaatgtaag aattgaagat attgatttat caatattttg ttttacatct tttgctaaat
      361 caacagtttt tgggctataa ccttttttat ctaaactctc aatatatgaa gaagttgagg
      421 ataaaagtgt agcttcaagt agtgaacaca taaaagataa aataagtgtt aaaagtaaat
      481 atgtaacaag tagtgtcatt ttttaaattc aattattgaa atatttttta aaatatgttt
      541 tatatctttt gcatttaaaa gatgtttttc ttcaagaact ttaaaaaaaa tctcacctct
      601 gcatttgtaa aaccatctgg ttctttctct tttatagatg gtgtttgacc atcttctaat
      661 cttattctat atagtttttt atttttaagt tttgcataat atactatgtc aatttgagtt
      721 tgagtaactt tttctaatgt tacaaaaact ctattactct ctagttccat ttcaaaatct
      781 ttataaaact ctttgaaaca atctatattt aaatatccaa tatataattt tgtgtaaata
      841 tcaaaatata aactcatttt agaatttgaa aaaaaactta aatcaatttt aaatttagga
      901 ttttttctag caccatttaa aatccattct aaagttggaa aatatgcaaa ttttttatat
      961 tttaaactaa aagcttctaa atacttctta ttttctggag cttttaatgt ctgtaaatca
     1021 tttttaactt ttttagcaaa aataattttt tctattaaat catcatcatc taaatctttt
     1081 gttacccaaa tcgttgcata tgatggaaaa ttgtttaatc caacagaacc agctgtcaaa
     1141 acaatcaaag catttatctc taaagatgga aatatcatct ttaaaagtgc atattttttt
     1201 gctaatcttt tatttaaact tgaagttttt tttgaagtac tcatctctac aaaatatagc
     1261 ttattttcag taaaaaacat tgcatcaaat tcgcttatat ctttatatgc tgatttaaaa
     1321 actatttgtg aagttctatc tattaaaagt ccacttttta taaacttgtt ttccatatct
     1381 tgatatggac cttttagtac aaaacctttt atatcatcat tatttaaagc ataatccatc
     1441 aaaagttcat atattatatt ttcataaatc tcacctttaa aactactatg agctgtaata
     1501 taagagatat tgtcttttga aatattattt ttaaatagag attttaaagt tttactatca
     1561 taagagtagt gaaaaagatt atcttttaat tcacctattt ctaaattttt tatcttattc
     1621 gtaattttaa attgcaaatc ttctcttttt ttcataatat caatgattat atcaaatatt
     1681 tcttgatata atccactttt aattaaagga tataaagtga aaaaactatt tttaatagat
     1741 at
//
LOCUS       DAOQAN010000275         1734 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_105447_length_1734_cov_2.994640, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000275 DAOQAN010000000
VERSION     DAOQAN010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105447_length_1734_cov_2.994640"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            229..765
                     /locus_tag="PKO10_04860"
     CDS             229..765
                     /locus_tag="PKO10_04860"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HNW66508.1"
                     /translation="MNTNHKSDFRQSCIKRLKFVALFSKYSRNKRVIKKLKSIIQISD
                     AKNILLYLPLDIEVDTTLLINELRREKKIVYVPFMEDDSFKIVKYRLPLHKKKFNIKE
                     PTNSFLKPNKIDLAVVPIVGIDALNKRIGFGKGMYDRFFYRLKYRPKIVFIQLELCKS
                     KEILSDNYDIEADYIITG"
     gene            772..>1734
                     /locus_tag="PKO10_04865"
     CDS             772..>1734
                     /locus_tag="PKO10_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rnase Y domain-containing protein"
                     /protein_id="HNW66509.1"
                     /translation="MEILLISVAFGAFSSLVTVFISKKINKAKFEVFIEQAKAKARVI
                     EHEAEVALKDAQLKAKFECDREFKNARKEYENMLFKIEKKEKELNEHLENELRLIKAQ
                     KEEISENNRKIAILKDGIEEQKRTYEQKTLEAIKILENASGLTKSEAKELMLKKVEED
                     SRAEIASIFRKKYKLAEQNSKQEVNNILSQAVTRYAGEFAAERLINNIPLNDEETKGK
                     IIGKEGRNIKALEMLLGVDIIIDDTPNTITVSSFNLYRRAIATRTIQELLEDGRIQPA
                     RIEEIVEKVKAELEEDLIKEGENAVMQLGLHKMHPELIKHIGKMK"
BASE COUNT          727 a          170 c          298 g          539 t
ORIGIN      
        1 tatcttgatt taaagatata tttatttgtg agttattaat tgtatttaaa ttaaattgct
       61 tttcagcgct atttattgta ggtttttgat ttttatcact ttttgaatca caagcagtaa
      121 aaaataaaat tggtaaaata gttaaaattg ctaaagtttt aaacttcatt ttaattcctt
      181 tagataaaat tgttttttag gtagagattt tacctaagtg aggcttaaat gaacacaaat
      241 cacaaaagtg attttcgaca atcgtgtatt aaaagattaa aatttgtggc acttttttca
      301 aaatactcaa gaaacaagag agttattaaa aaattaaaga gtattataca aattagtgat
      361 gcaaaaaata ttttattgta tcttccactt gatatagaag tagatacaac acttttaata
      421 aatgagttaa gaagagaaaa aaaaatagtt tatgtaccat ttatggaaga tgatagtttt
      481 aaaatagtaa agtatagact acctcttcat aaaaaaaagt ttaatattaa agagcctaca
      541 aactcttttt taaaaccaaa taagattgac ttagcagtag ttcctattgt tggaatagat
      601 gctttaaata aaagaatagg atttggtaaa ggtatgtatg atagattttt ttatagatta
      661 aaatacagac caaaaatagt ttttatacaa cttgaacttt gtaaaagtaa agaaatttta
      721 agtgataatt acgatattga agcagattat ataattacgg gataggttaa aatggagatt
      781 ttattaatat ctgttgcatt tggggctttt agttcacttg ttacagtttt tatttcaaag
      841 aagataaata aagctaagtt tgaagttttc atagaacaag caaaggcaaa agcaagagtt
      901 atagaacacg aagcagaagt tgcactaaaa gatgcacaat taaaagctaa atttgagtgt
      961 gatagagagt ttaaaaatgc tagaaaagag tatgaaaata tgctttttaa gatcgaaaag
     1021 aaagaaaaag agctaaatga gcatcttgaa aatgaattaa gacttattaa agcacaaaaa
     1081 gaagaaatta gcgaaaataa tagaaaaatt gccatcttaa aagatggtat tgaagagcaa
     1141 aaaagaactt atgagcaaaa aactcttgaa gctataaaaa tcttggaaaa tgctagtgga
     1201 cttacaaaaa gtgaagcaaa agagctgatg ctaaaaaaag ttgaagagga ttcaagagct
     1261 gaaattgctt caatttttag aaaaaaatat aaattagcag aacaaaattc aaaacaagag
     1321 gtaaataata ttttatctca agcagttaca agatatgcag gagagtttgc agcggagcga
     1381 cttataaata atattccttt aaatgatgaa gagacaaaag ggaaaattat tggtaaagaa
     1441 ggacgtaata taaaagctct tgagatgctt ttaggagttg atattataat agatgatact
     1501 ccaaatacta tcactgtttc atcttttaat ttatatagac gtgctattgc tacaagaaca
     1561 attcaagaac tacttgaaga tggaagaatt cagcctgcaa gaattgaaga gattgttgaa
     1621 aaggttaaag cagaattaga agaagatttg atcaaagaag gtgaaaatgc agtaatgcag
     1681 ttaggtctgc ataaaatgca tcctgagttg attaaacata tcggtaaaat gaaa
//
LOCUS       DAOQAN010000276         1731 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_105704_length_1731_cov_3.302506, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000276 DAOQAN010000000
VERSION     DAOQAN010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105704_length_1731_cov_3.302506"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            171..1406
                     /locus_tag="PKO10_04870"
     CDS             171..1406
                     /locus_tag="PKO10_04870"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNW66510.1"
                     /translation="MVSKFEKMFQNTNEVLKTNKELLYFDEVTKINNRKYFILKANEY
                     LDKDSSNNKGFIVSLSIKLDVINKTYGFIKTNEILFKLASKLKNSFNSDNDIVVRMNG
                     SEFLALVPNAKEEDVKNALEKLIIDLKAIVELEDNIFIGLCKYENEESLRTLFTKIDY
                     TLSQAKVNSDKDYFYVTHMENIKTKEEWINILNVSLKEEFFRLIHRDIVDINSKEEYL
                     KTISFELDFKGETIRYGEFIASVLEQNRLDEVYLHVIEKVFKQNKANEFVSIQLPTLF
                     IEKLSSYANLKELLNKYKHISKNIIFEIEEEAFNKNFNSTLMYISLFKEFGFNFAIFN
                     FIANSDDYNYLKELRPLYIKASKFFLLESRQSLNMLKILTQSLDIKLVATSVDEIEEI
                     KTLEEIGINAISGSVMSKL"
     gene            1491..>1731
                     /locus_tag="PKO10_04875"
     CDS             1491..>1731
                     /locus_tag="PKO10_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HNW66511.1"
                     /translation="MIVKIDLTNDNSYEIYIEKLKALSFDRKVVVVTNPTVAGFHLEY
                     LKSKLTAKELSICTIKDGEEYKNMQTLEDILSSCFE"
BASE COUNT          696 a          173 c          279 g          583 t
ORIGIN      
        1 atgaatgcgc gggcgtttac ggtgggccga aatgtggtat ttggtgaagg ggaatatgtg
       61 ccgcagacgg tgactggtag gaagctgctg gcgcacgagc taacgcatgc attgcaacag
      121 agcaaccatt tacagatgag tttaaaactt taacaactag tataaatagt atggtttcta
      181 aatttgaaaa aatgtttcaa aatacaaatg aagtattaaa gacaaataaa gaacttttat
      241 attttgatga agtaacaaaa ataaataata gaaaatattt tatattaaaa gcaaatgagt
      301 atttagataa agatagctca aataataaag gttttatagt atctctttca ataaaacttg
      361 atgtaataaa caaaacttat ggatttataa aaacaaatga gattttgttt aaattagctt
      421 caaagttaaa aaatagtttt aatagtgaca atgatattgt agttagaatg aatggttcag
      481 aatttttagc acttgttcca aatgctaaag aagaagatgt aaaaaatgct ttagaaaaac
      541 tgataattga tttaaaagct attgtagagc tcgaagataa tatttttatt ggtctttgta
      601 aatatgaaaa tgaagagagt ctaagaactc tatttacaaa aatagactac acactttctc
      661 aagcaaaagt aaatagcgat aaagattatt tctatgtaac tcatatggaa aatattaaaa
      721 caaaagaaga gtggataaat atattaaatg tatctttaaa agaggagttc tttaggctta
      781 tacatagaga tattgttgat attaactcaa aagaagagta tttaaaaaca attagttttg
      841 agcttgattt taaaggcgag acaataagat atggcgaatt tattgcctct gttttagaac
      901 aaaataggtt agatgaagta tatttacatg ttattgaaaa agttttcaaa caaaataaag
      961 caaatgagtt tgtatcgatt caacttccta ctttatttat tgagaaatta agtagttatg
     1021 caaatttaaa agagttatta aataagtata aacatatttc aaaaaatatt atttttgaaa
     1081 ttgaagaaga ggcatttaat aaaaatttca atagcacttt gatgtatatt tcactattta
     1141 aagagtttgg atttaacttt gctatattta attttattgc aaatagcgat gattataact
     1201 atttaaaaga gttaagacca ctttatatta aagcttcaaa attcttcttg cttgaatcaa
     1261 gacagagttt gaatatgtta aaaatactta cacaatcttt agatattaaa cttgttgcta
     1321 caagtgttga tgaaattgaa gagataaaaa ctttagaaga gataggaata aacgcaattt
     1381 ctggctctgt tatgtcaaaa ttataaaaac tactatttga ccaaaaaagg ctttttttag
     1441 ctttttttgg ttaatatcta tttctactta tatcaaatga aagataaatt atgattgtaa
     1501 aaatagactt aacaaatgat aattcatacg aaatttatat cgaaaaatta aaagcattga
     1561 gctttgatag aaaagttgtt gttgttacta accctacggt tgctggtttt catttagagt
     1621 atttaaaatc aaaattaaca gcaaaagagt tatctatttg cacaattaaa gatggtgaag
     1681 agtataagaa tatgcaaact ttagaagata ttttaagttc atgctttgaa g
//
LOCUS       DAOQAN010000277         1728 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_106071_length_1728_cov_1.742977, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000277 DAOQAN010000000
VERSION     DAOQAN010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106071_length_1728_cov_1.742977"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            347..1381
                     /gene="recA"
                     /locus_tag="PKO10_04880"
     CDS             347..1381
                     /gene="recA"
                     /locus_tag="PKO10_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006565321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HNW66512.1"
                     /translation="MKKDKDKALEQVMADIEKQFGKGAIMKLGERGVKNIDVVSSGSI
                     TLDQALGVGGYPKGRIIEIFGPESSGKTTIALHAIAEVQKVGGKAAFIDAEHALDPVY
                     AKKLGVDIDELLLSQPDTGEQALEIVEALVRSEAISIIVIDSVAALVPQAEIEGEMGD
                     SHVGLQARLMSQALRKLSGAISKTNTICVFINQLREKVGIMFGNPETTPGGRALKFYS
                     SIRLDVRRGEQIKAGTDILGNKTVIKVVKNKVAPPFKTAEVEIIYGEGISRTGEVVDL
                     ASNVGIIDKSGAWFSYKGEKIGQGKENVKILFKNNPKLYKEIEEKVRADLFNTAESKA
                     KEIPIQIEEK"
     gene            1459..>1728
                     /locus_tag="PKO10_04885"
     CDS             1459..>1728
                     /locus_tag="PKO10_04885"
                     /inference="COORDINATES: protein motif:HMM:NF013565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HNW66513.1"
                     /translation="MSLKVYNTISREKEDFVPLNGNNVGMYVCGPTVYGLPHLGHAKS
                     YISFDVVYRYLKYLNYKVKYVQNITDVGHLVGDADAGESKVEKQAI"
BASE COUNT          697 a          178 c          312 g          541 t
ORIGIN      
        1 tactcttatc ttcttttata acttaccatt tgagttatgg aatatagata ttgctactat
       61 atgtatacta attgctactg tattatctgt agttagtggt atagaatact atgtaaaaaa
      121 taaaaaatat tttgctgata taaaataaaa tataaagatt aaaattaaat ttttaatctt
      181 ttttatttga taaaaaccgc ctaaataaag gaaaagatgc accaaaattt aattaataaa
      241 ctaatagtaa atacaaaatt gaaatataac aaacatgtgt ttgtaaaaaa atgttgacaa
      301 agaattcttt ttgttttaca attaaatcat aaaggagaaa cataacgtga aaaaagacaa
      361 agataaagca cttgaacagg taatggccga tatcgaaaaa caatttggta aaggcgcaat
      421 tatgaaatta ggagaaagag gagtaaaaaa tatagatgtt gtatctagtg gatcaattac
      481 attagatcaa gctttaggag taggtggtta tcctaaagga agaattattg aaatatttgg
      541 accagaatct agtggtaaga caactattgc attacacgct attgctgaag tacaaaaagt
      601 tggtggcaaa gctgcattta tagatgctga gcatgcatta gatcctgtat atgctaaaaa
      661 attaggtgta gatattgatg aattattgtt atctcaacct gatactggtg aacaagcatt
      721 agaaattgtt gaagcacttg ttagaagtga agcaataagt ataatagtaa ttgactctgt
      781 tgctgcatta gttcctcaag ctgaaattga gggtgaaatg ggagattctc atgttgggct
      841 tcaagctaga cttatgtcac aagcacttag aaaattaagt ggtgcgatta gtaaaactaa
      901 tactatttgt gtatttatta accaattaag agaaaaagtt ggaattatgt ttggaaatcc
      961 tgaaacaact cctggaggaa gagcacttaa attctattct tctataagac ttgatgtaag
     1021 aagaggagaa caaattaaag ctggaactga tatacttgga aataaaactg ttattaaagt
     1081 agttaaaaat aaagttgctc ctccatttaa aacagctgaa gtagaaataa tatatggaga
     1141 aggaatatct agaactggtg aagtagttga tttggcttct aatgttggaa taattgataa
     1201 aagtggagca tggtttagtt acaaaggtga aaagattgga caaggaaaag aaaatgttaa
     1261 gatcttattc aaaaataatc ctaaactata caaagaaata gaggaaaaag ttagagctga
     1321 tttatttaat acagctgaat ctaaagctaa agagattcca attcaaatag aagaaaaata
     1381 atttcttcta tttttaataa tataataata gaataataat taaaaatatg ttaaaatcat
     1441 actgaaagag gtgtaactat gtcacttaaa gtttataata caatatcaag agaaaaagaa
     1501 gattttgttc ctttaaatgg gaataatgtt ggtatgtatg tttgtggacc tacagtgtat
     1561 ggtttaccac atttaggaca tgcaaaaagc tatattagtt ttgatgtagt atatagatat
     1621 cttaaatatc taaattataa agttaaatat gtacaaaata taactgatgt aggtcattta
     1681 gttggagatg ctgatgcagg agagtcaaag gtagaaaaac aagctata
//
LOCUS       DAOQAN010000278         1727 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_106156_length_1727_cov_1.975478, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000278 DAOQAN010000000
VERSION     DAOQAN010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106156_length_1727_cov_1.975478"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            60..350
                     /locus_tag="PKO10_04890"
     CDS             60..350
                     /locus_tag="PKO10_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66514.1"
                     /translation="MTLEELREDLDIVLMFIRESTLTEAEFESTESYFPNLENVELLQ
                     EYQALFFVLSKRGGEGNSIRKLKYYFYIKLEDEAVLNLETDYNNVIIGAVLD"
     gene            376..813
                     /locus_tag="PKO10_04895"
     CDS             376..813
                     /locus_tag="PKO10_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66515.1"
                     /translation="MNDFKKEFEKQTFVVGILENVPGKIPLSKKAGYRTVEGVRARKI
                     KGNARIGMQDLGGILQKKYNWLLTPLKQEKNIESIVELIADYVFKRDNIFQKLAEVFK
                     EHIKEKKYRPNKISTEKRKGFNHSLVDTGSFLKNIKVKLEKRK"
     gene            867..1247
                     /locus_tag="PKO10_04900"
     CDS             867..1247
                     /locus_tag="PKO10_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66516.1"
                     /translation="MQKVLFCKPSIEKEKSFFCWVEKSRIRKTAEGKAFGTVNGYIAI
                     IGQNAENPSWFFQRRIEKADRLILNRFSFSSIENNIEIESFDKSFNGFYVEWTYFYSQ
                     EYDPIRYKIAGVDFIFNVIKKFFN"
     gene            1259..1528
                     /locus_tag="PKO10_04905"
     CDS             1259..1528
                     /locus_tag="PKO10_04905"
                     /inference="COORDINATES: protein motif:HMM:NF019282.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide modification associated
                     domain-containing protein"
                     /protein_id="HNW66517.1"
                     /translation="MEKIEKHKSICEKLNKLYEKKNHDYGDSFSTLRKKHNNAILIRL
                     SDKLNRLESLYLKKDIKVEDESIVDTLIDIANYAIMEIIEIEEQK"
     gene            1528..>1727
                     /locus_tag="PKO10_04910"
     CDS             1528..>1727
                     /locus_tag="PKO10_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66518.1"
                     /translation="MDILLDYVFKVNLQEALPIANKNYLRQVAVIVKPKSAVDNGCYS
                     ITNKSDITTYTDNVDVLQLFNAG"
BASE COUNT          723 a          143 c          325 g          536 t
ORIGIN      
        1 aaaatctata gctctctttt tgggtttcta agaagagagc tataaaaaaa gaggatatta
       61 tgacattaga agaattgaga gaggatttag atattgtttt gatgtttatt agagaatcta
      121 ctttgacaga agcagaattt gaaagcactg aaagctattt tcctaatctt gaaaatgttg
      181 agcttttgca agaatatcaa gctttatttt ttgttttgtc aaagagaggc ggagaaggga
      241 attctattag aaaattgaaa tattatttct atatcaagtt agaagatgaa gcagttttga
      301 atcttgaaac agattataac aatgtaatta taggagcagt gcttgattag ttttaagata
      361 gatagcaaaa aatttttgaa tgattttaaa aaagaatttg aaaagcaaac ttttgtagta
      421 ggaattttag aaaatgttcc tggaaaaatt ccactttcaa aaaaggctgg atatagaaca
      481 gtcgaaggag taagagcaag aaaaataaaa ggaaatgcaa gaattggaat gcaggactta
      541 ggcggaatat tacagaaaaa atataattgg ctattgactc cacttaagca agaaaaaaat
      601 atagaaagca ttgttgagtt aatagctgat tatgttttta agcgagataa tatttttcaa
      661 aagctcgcgg aagtttttaa agagcatata aaagaaaaaa aatatagacc taataagatt
      721 tctacagaaa aaagaaaagg atttaatcat tcgctagttg atacaggaag ttttttgaaa
      781 aatatcaaag taaaattgga aaaaaggaaa taaaaattaa aatgtttcac tctagccttg
      841 aacaagattt aaagagaatt tttggagtgc aaaaagtttt attttgtaag cctagtattg
      901 aaaaagaaaa aagttttttt tgttgggttg agaaaagcag aattagaaaa acagcagaag
      961 gaaaagcatt cgggacagtg aatggatata ttgcaataat tgggcaaaat gcagaaaatc
     1021 cgagctggtt ttttcaaaga cgaatagaaa aagctgacag attaatattg aatagattta
     1081 gtttttcttc aatagagaat aatatagaaa tagaaagttt tgataaaagt tttaatggct
     1141 tttatgttga atggacatac ttttacagtc aagaatatga tccaattcgg tataaaatag
     1201 caggtgtaga ttttatattt aatgtgataa aaaaattttt taattaggag aaaaaacttt
     1261 ggaaaaaata gaaaagcata aaagtatttg tgaaaagcta aacaaattat atgaaaaaaa
     1321 gaatcatgat tatggtgata gtttttctac attaaggaaa aaacataata atgcaatatt
     1381 gattagattg tcagataaat taaataggtt agaaagttta tatttgaaaa aagatataaa
     1441 agttgaagat gagagtattg tagatactct aatagatata gcaaattatg cgattatgga
     1501 aataatagaa atagaggagc aaaaataatg gatatattat tagattatgt atttaaggtt
     1561 aatttacaag aagctttgcc aatcgcaaat aaaaattatt tacggcaggt tgcagttata
     1621 gtaaagccaa aaagtgctgt tgataatggg tgttattcta ttacaaataa aagtgatata
     1681 acgacatata cagataatgt tgatgtattg caattattta atgcagg
//
LOCUS       DAOQAN010000279         1726 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_106247_length_1726_cov_2.030521, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000279 DAOQAN010000000
VERSION     DAOQAN010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106247_length_1726_cov_2.030521"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            9..116
                     /locus_tag="PKO10_04915"
     CDS             9..116
                     /locus_tag="PKO10_04915"
                     /inference="COORDINATES: protein motif:HMM:NF037635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66519.1"
                     /translation="MKNIFAMSKKAFKATLTKLIDEKKINLENDRICLI"
     gene            213..752
                     /gene="tpx"
                     /locus_tag="PKO10_04920"
     CDS             213..752
                     /gene="tpx"
                     /locus_tag="PKO10_04920"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HNW66520.1"
                     /translation="MATVKFKGELEVNLSGTELNVGDIAPVVTGVGADLSDITIGGQR
                     GMAQVIIAVPSLDTGVCAAEARRFNVEASKLDHVEVVIVSMDLPFAMKRFCTTEGIEN
                     LTVGSDFRAKAFAKSYGVLQANGPLAGLTARAVFIVNPSGKITYKEIVPEITQEPNYD
                     AVLKAITEASSTSCCGSCH"
     gene            857..1471
                     /locus_tag="PKO10_04925"
     CDS             857..1471
                     /locus_tag="PKO10_04925"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNW66521.1"
                     /translation="MKKIIKTIFVLMLSFSFSNASLVEDGYIELEKGNVLEAANIFKV
                     SCEKGAFAGCYNLGLMYFKGEHIAKNYTKAEEYFSKACKEGHQNACYNLANMYEKGLG
                     VEKDNFKAVDLYIKTCKNGVSSSCFNLAIMYKNGNGVEKDDFRAVDFYTKACEMNHQK
                     ACYNLGVMYLEGDFLAINKTKAKEYFEKSCELGYSNACKELGKL"
     gene            1544..>1726
                     /locus_tag="PKO10_04930"
     CDS             1544..>1726
                     /locus_tag="PKO10_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HNW66522.1"
                     /translation="MTSWYKKFASQPHQPFFTNGVIFFILFITLFAFAYSNSIKLDTS
                     ILTYHAYALIFVVFIQF"
BASE COUNT          637 a          177 c          298 g          614 t
ORIGIN      
        1 ctgaagatat aaaaaatata tttgctatga gtaaaaaagc ttttaaggcg actttgacaa
       61 agctaattga tgagaaaaaa ataaacttgg aaaatgatag aatttgtttg atttaaatta
      121 agacaaatct tatcctaatt gaatataatt tcagttaaat atcttaaata agatttttta
      181 attgattgaa atttatataa aaaggaaata aaatggcaac agtaaaattt aaaggtgaat
      241 tagaagtaaa tttaagtgga acagagttaa atgttggaga tatagctcct gttgtaactg
      301 gtgttggagc agatttaagt gatattacta ttggtggaca acgaggaatg gctcaagtta
      361 ttattgcagt tccatctctt gatactggtg tttgtgcagc agaagctaga agatttaatg
      421 ttgaagcttc aaaattagat catgtagaag ttgttattgt atctatggat ttaccatttg
      481 ctatgaaaag attttgtaca actgaaggga ttgaaaactt aacagttggt tcagatttta
      541 gagcaaaagc atttgcaaaa tcttatggtg ttttacaagc gaatggtcct ttagcaggac
      601 ttactgcaag agctgtgttt atagtaaatc catctgggaa aattacatat aaagagattg
      661 ttcctgaaat aactcaagaa ccaaattatg atgctgtttt aaaggcaata actgaagcat
      721 cttctacttc ttgttgtgga agttgtcact aaaaaacttt tttaggtaag aatttcttac
      781 ctaaaatact ctttttttat ctactattaa ttaaaaagct gtatcattat ttaaatctct
      841 aaagaaaggg ttagctatga aaaaaataat taaaactata tttgtcttaa tgttgagttt
      901 ctcattttca aatgcttcat tggtagaaga tggttatatt gagcttgaaa aaggaaatgt
      961 tttagaagct gcaaatatat ttaaagtttc atgtgaaaaa ggtgcatttg ctggatgtta
     1021 taatctaggt ttaatgtatt ttaaaggtga acatatcgcc aaaaactata caaaagcaga
     1081 agagtatttt tcaaaagctt gtaaagaagg tcatcaaaat gcttgttata atttagcaaa
     1141 tatgtatgaa aaaggtttag gtgtagaaaa agataacttt aaagctgttg atttatatat
     1201 aaaaacttgt aaaaatggag taagttcttc ttgttttaat ttagcaatta tgtataaaaa
     1261 tggaaatgga gtggaaaaag atgattttag agcagttgat ttttatacaa aagcttgtga
     1321 aatgaatcat caaaaagctt gctataatct tggggttatg tatcttgaag gtgatttttt
     1381 agctataaat aaaacaaaag caaaagagta ttttgaaaaa tcttgtgagc ttggatatag
     1441 caatgcttgt aaagagcttg gaaaacttta aattattgtt tgaaatcaag ctaatttata
     1501 aaaaaaatag ttaaaatctc ttaatttata ttaggagatt ttaatgactt cgtggtacaa
     1561 aaagtttgct tcgcaaccac atcaaccatt ttttacaaat ggggtaatat tttttatact
     1621 tttcataact ctatttgctt ttgcttattc aaatagtata aaattggata catcaatttt
     1681 aacatatcat gcatatgctc ttatttttgt agtatttatt cagttt
//
LOCUS       DAOQAN010000280         1724 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_106399_length_1724_cov_2.705812, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000280 DAOQAN010000000
VERSION     DAOQAN010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106399_length_1724_cov_2.705812"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            90..410
                     /locus_tag="PKO10_04935"
     CDS             90..410
                     /locus_tag="PKO10_04935"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HNW66523.1"
                     /translation="MFAVIFEVEINQERKDEYLKIASILKEQLVNQKGFISVERFQSL
                     IDEKKLLSLSYWEDEEAILAWKKNIDHMSAQKKGRESIFKDYKINIAKIQKSYTLKTS
                     DFDK"
     gene            419..1504
                     /locus_tag="PKO10_04940"
     CDS             419..1504
                     /locus_tag="PKO10_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013135042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HNW66524.1"
                     /translation="MATLLKNIYSQEFIKNLSNILKNSYKDFKIDEFKKAIFTNAWEN
                     FELKQRMRHISKTLYEFLPFSYKEQIEILIEVKKDFKALEAMIFQDFVEVFGLDDFEI
                     SMKALELFTIDSSSEFAIRQFIIKYEDKTINQMKIWAKSKNEHIRRLASEGSRPRLPW
                     AVALPNFKKNPQKVFEIIELLKNDSSKYVQKSVANNLNDISKDNPKMVIDFVKKNLNI
                     SKNLDWICKHGSRTLLKKGDKEVLDLFNFNKSHHINLTNFCCDKTIFLNEDFNFSFEI
                     NSDEPIGNIRVEYVIYYLKSNSNHNKKVFMISQNSIESTKKRFQKKQSFKNMTTRKHY
                     IGTHFIGILINGVEVLKEEFFLHDPSN"
     gene            1488..>1724
                     /locus_tag="PKO10_04945"
     CDS             1488..>1724
                     /locus_tag="PKO10_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HNW66525.1"
                     /translation="MTPAINLLKKHKCDFKIHKYEHDPACTNFGDEAVEKLGLDANQV
                     YKTLLAELTPKELVVCVIPVSKQLSLKEVADIFGV"
BASE COUNT          729 a          163 c          256 g          576 t
ORIGIN      
        1 agtagatatt taaaatcttt caatttgtaa tatttattaa taaaattgag attttatact
       61 ataataaaat aaattttaaa ggaaagttta tgtttgcagt tatttttgaa gttgaaatta
      121 atcaagaaag aaaagatgaa tatttaaaaa ttgcttcaat attaaaagag caattagtaa
      181 atcaaaaagg ttttataagt gttgaaagat ttcaatcttt aatagatgaa aaaaaacttc
      241 tatcattatc atactgggaa gatgaagaag ctattcttgc ttggaaaaaa aatattgacc
      301 atatgtctgc tcaaaaaaaa ggaagagagt ctatttttaa agattataaa ataaatattg
      361 ccaaaattca aaaatcatac actttaaaaa ctagtgattt tgataagtaa gtatattaat
      421 ggctacttta ctaaaaaata tatattcaca agagtttata aaaaatctat caaatatttt
      481 gaaaaatagc tacaaagatt ttaaaataga tgagtttaaa aaagctattt ttacaaatgc
      541 ttgggaaaat tttgagctaa aacaaagaat gcgacatatc tcaaaaactt tgtatgagtt
      601 tttaccattt tcatataaag agcaaattga gattttaata gaggtaaaaa aagatttcaa
      661 agctttggaa gctatgattt ttcaggattt tgttgaagtt tttgggcttg atgattttga
      721 aatttcaatg aaagctttgg aactttttac aatagattca agtagtgagt ttgcaataag
      781 acaatttata attaagtatg aagataaaac tataaatcag atgaaaattt gggcaaaaag
      841 taaaaatgag catataagaa gattagcaag tgaaggaagc agacctagac ttccttgggc
      901 tgttgctttg ccaaatttta agaaaaatcc acaaaaggta tttgagataa ttgagctact
      961 taaaaatgac tcttcaaaat atgttcaaaa aagtgtagca aataacttaa atgatatatc
     1021 aaaagataat ccaaaaatgg taatagattt tgtaaaaaag aatttaaata tttcaaaaaa
     1081 tcttgattgg atttgtaaac atggctctcg tacgcttttg aaaaaaggag ataaagaggt
     1141 attagattta tttaatttta ataaatctca tcatataaat ctcacaaatt tttgttgtga
     1201 taaaactata tttttgaatg aagattttaa tttttcattt gagataaatt cagatgaacc
     1261 tattggaaat ataagagttg aatatgttat ttattattta aaatcaaaca gtaatcataa
     1321 taaaaaagtt tttatgataa gtcaaaatag tatagaatca acaaaaaaaa gatttcaaaa
     1381 aaaacagagt tttaaaaata tgacaactag aaaacactat ataggaactc attttatagg
     1441 aattttgata aatggagtag aagttttaaa agaggagttt ttcttacatg accccagcaa
     1501 ttaatttact aaaaaaacat aaatgtgatt ttaaaattca taaatatgaa cacgatccag
     1561 cttgtacaaa ttttggagat gaagcagtag aaaaactagg tttagatgca aatcaggttt
     1621 ataaaacttt acttgcagaa cttactccaa aagagttggt cgtttgtgta attcctgttt
     1681 caaaacagct tagtttaaaa gaagttgcag atatttttgg agta
//
LOCUS       DAOQAN010000281         1719 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_106885_length_1719_cov_2.951322, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000281 DAOQAN010000000
VERSION     DAOQAN010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106885_length_1719_cov_2.951322"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="PKO10_04950"
     CDS             complement(<1..244)
                     /locus_tag="PKO10_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66526.1"
                     /translation="MSVLQILTKDEIKNFESAPKFDLLAKNHFFKMTDALNHMTMEMK
                     NDSNKILFILMYGYFKATNKFFDIQRDDENYLYIATR"
     gene            406..1017
                     /locus_tag="PKO10_04955"
     CDS             406..1017
                     /locus_tag="PKO10_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HNW66527.1"
                     /translation="MFIGYARVSKSDNSQVLDLQIDALKNAGVDENNIYTDKISGSKD
                     ERPGLDNCIKALRDGDTLVVWKLDRLGRNLKHLISIVEDLAKRNIGFKVLSGQGVNID
                     TTSPAGKMIFSIFGALAEFERELIRERTKAGIAAARARGRMGGRKFNLTKAQVRLAEV
                     AMKNRDTSVTELCKELKITRATLYKYISPTGELREYAKRVLEK"
     gene            1082..1264
                     /locus_tag="PKO10_04960"
     CDS             1082..1264
                     /locus_tag="PKO10_04960"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNW66528.1"
                     /translation="MPSDIIEKIFDPYFTTKENDEGTGIGLYMCKQIIDGMTGKIQVI
                     NVEYEYEAQRYYGAER"
     gene            1278..1439
                     /locus_tag="PKO10_04965"
     CDS             1278..1439
                     /locus_tag="PKO10_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66529.1"
                     /translation="MQQIQINNKYLEFQNGIISYRSPTVEKVISYNKDDEKSFIAALM
                     QIINLIEFE"
     gene            1479..>1719
                     /locus_tag="PKO10_04970"
     CDS             1479..>1719
                     /locus_tag="PKO10_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66530.1"
                     /translation="MTCKNRVKPKLLRDVKTEALLVFIRTTLEQSFYQIDNVGYNFLL
                     GNDEDNEYVYSTLKKLLIQLEDTVINSSYLRSLIEN"
BASE COUNT          670 a          196 c          282 g          571 t
ORIGIN      
        1 ttctagtagc tatgtacaaa taattttcat catctctttg aatatcaaaa aatttattgg
       61 tagctttaaa atacccatac attaaaatga acaaaatttt atttgaatca tttttcatct
      121 ccattgtcat atggttaaga gcatcagtca ttttaaaaaa gtgatttttt gcaagcaaat
      181 caaattttgg agctgattca aagtttttaa tttcatcttt tgtgagaatt tgtaatactg
      241 acattgtctt tccttaaaat tgtaatttat acttatttta tatgacattt aatataattt
      301 gaataattta tatgttaatt tacattatat aggttcttta gagagatatt tttgaatgta
      361 tattaaacgg tcgtttgaga tacacttttt tacgaggaga aaaaaatgtt cattggatat
      421 gccagagttt caaaatcaga taattcacaa gtattagatt tacaaatcga tgcacttaaa
      481 aatgctggag ttgatgaaaa caatatttat acagacaaaa tctcaggaag taaagatgaa
      541 agacctggat tagataattg cattaaagct ttaagagatg gtgatacttt agttgtgtgg
      601 aagctagata gacttggaag aaatctaaaa catcttatat ccatagttga ggatcttgca
      661 aaaagaaata ttggatttaa agtgctaagt ggtcaagggg ttaatattga tacaacttca
      721 cctgctggta aaatgatatt ttcaatattt ggagctttag ctgagtttga aagagaactg
      781 attcgtgaaa gaacaaaagc tggaattgca gctgcaaggg caagaggtag aatgggtgga
      841 agaaaattca atcttacaaa agctcaagtg agacttgcag aggttgccat gaaaaataga
      901 gatacgagtg tcactgaact ttgcaaagaa ttaaaaataa caagagcaac tttatacaaa
      961 tatatttcac caactggtga gctaagagag tacgctaaaa gggtacttga gaaataaagc
     1021 ttaagctatc tttaaatttc tttgtcgcat ttttcccaaa tgttcccaag tagccaatgg
     1081 tattccttct gatattattg aaaaaatatt tgatccttat tttacaacaa aagaaaatga
     1141 tgaaggtaca ggaattggac tttatatgtg taaacaaata attgatggaa tgactggaaa
     1201 aattcaagtt ataaatgttg agtatgagta tgaagcacaa agatattatg gtgctgaaag
     1261 atagtaagga agaatagatg caacaaatac aaattaacaa taaatattta gaatttcaaa
     1321 atggtattat ttcttatcgt tctccaactg tagaaaaagt tattagttat aacaaagatg
     1381 atgaaaaatc ttttatagca gcattaatgc aaataatcaa tttaattgaa tttgaataat
     1441 agtatcactt aatagtgata tcctttaaga aaatatatat gacctgtaaa aatagagtaa
     1501 aaccaaaact actaagagat gtaaaaacag aagctctctt agtatttatt agaactacac
     1561 tagagcaatc tttttatcaa atagataatg tagggtacaa ctttttatta ggtaatgatg
     1621 aagataatga atatgtatat tcaactttaa aaaaactatt aattcaatta gaagatactg
     1681 taataaattc ttcatactta agaagtttaa ttgaaaatt
//
LOCUS       DAOQAN010000282         1719 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_106896_length_1719_cov_2.762019, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000282 DAOQAN010000000
VERSION     DAOQAN010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106896_length_1719_cov_2.762019"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="PKO10_04975"
     CDS             <1..256
                     /locus_tag="PKO10_04975"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HNW66531.1"
                     /translation="IKSDNQTVDVLLKKNGKIKIFKGKKADVSNTHGTGCSFSASIAS
                     LIARDKSLCKSIKKSKQYIYKAIKNAPNFGRGNGPINHIL"
     gene            316..1554
                     /locus_tag="PKO10_04980"
     CDS             316..1554
                     /locus_tag="PKO10_04980"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012857083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HNW66532.1"
                     /translation="MKEFLQKAKESSRVVATLSTAIKNKTLLEFADALEENSFFIIEE
                     NIKDMKLAKQLDLSSAMQDRLYLNDSRIQDMANAIRQIASQTEPVGRVLDGWLTKDNL
                     NIQKVSIPIGVIAIIYESRPNVTSDTAALCFKSGNVCVLKGGKEAENSNRAIAKIIQD
                     VLEKNNLPKEIVSLLPDSSREGVAKLIVEDKYVDLIVPRGGEALIKFITQNSSIPVIK
                     HDKGVCHTFIDKDANATKSINIVVNAKCQRPSACNSLETLLVHEEIASYILPGLQEEL
                     SAHGTILKGCPKTLGYIKIAPAKEEDFDIEYLENILNIKVVENLNEAIDHISKHGSGH
                     SEAILSENYTAINKFLNEVDAACVYANASTRFTDGGEFGLGAEVGISTNKLHSRGPMG
                     INDLTTFKYKIYGQGQVRTK"
BASE COUNT          685 a          223 c          292 g          519 t
ORIGIN      
        1 aataaaaagt gataatcaaa cagtagatgt tttgttgaaa aaaaatggga aaattaaaat
       61 atttaaaggt aaaaaagctg acgtttcaaa tactcatgga acaggttgta gttttagtgc
      121 ctctattgct tcacttattg caagagataa atctctttgt aaaagtataa aaaaatcaaa
      181 acaatatatc tacaaagcta taaaaaatgc tccaaatttt ggaagaggaa atggacctat
      241 aaatcacatt ttataaacaa agcctataca aagccaaact ttagtatatt tacacaaaat
      301 ttttaggaaa aatctatgaa agaatttcta caaaaagcaa aagagagtag tcgtgttgta
      361 gcaactctta gcacagctat aaaaaacaaa actcttctag aatttgcaga tgcacttgaa
      421 gaaaactcat ttttcattat tgaagaaaat ataaaagata tgaaactagc aaagcagctt
      481 gatttaagct ctgcaatgca agatagatta tacttaaacg atagtagaat tcaagatatg
      541 gcaaatgcta taagacaaat tgcttctcaa acagaaccag ttggaagagt tttagatggt
      601 tggcttacta aagacaactt aaatattcaa aaagtatcaa ttcctatcgg tgttattgca
      661 atcatatatg agagtcgtcc aaatgtaaca agcgatacag cagcgctttg ttttaaaagt
      721 ggaaatgttt gtgttttaaa aggtgggaaa gaggctgaaa attcaaatag agcaatagct
      781 aaaataatac aagatgtttt agaaaaaaac aatcttccaa aagagatagt ttcactactt
      841 cctgatagta gccgtgaagg tgttgcaaaa ttgattgttg aagataaata tgtagatttg
      901 atagttccaa gaggtggtga agctttaatt aaatttatca ctcaaaactc tagtatacct
      961 gtaatcaaac atgacaaagg agtttgtcac acttttatag acaaagatgc aaatgcaaca
     1021 aaatctataa atattgtagt aaatgctaaa tgtcaaagac caagtgcttg caactctcta
     1081 gaaactcttt tagttcatga agagatagct tcttatatat taccaggact tcaagaagag
     1141 cttagtgcgc atggaactat tttaaaaggt tgtccaaaaa ctttaggata tataaaaatt
     1201 gctccagcta aagaggaaga ttttgatata gagtatttag aaaatatttt aaatataaaa
     1261 gtcgtagaaa atttaaatga agcgatagat cacatatcaa aacatggttc aggacactct
     1321 gaagcaattt taagtgaaaa ttatactgcc ataaataaat ttttaaatga agttgatgca
     1381 gcttgtgtat atgcaaatgc aagtactaga tttacagatg gaggagagtt tgggcttggt
     1441 gctgaagttg gaatttctac aaacaaactt cactcaagag gacctatggg aataaatgat
     1501 ttaacaacat ttaaatataa aatttacgga caaggacaag ttagaacaaa atgagtacaa
     1561 atagactttt tttaatattt tgtagtgtta ttgcaacact atttttttgg cactatttag
     1621 gtgaaaaggc aattttaaaa gataattcaa atagctttga taaacttcaa tgtgtatctt
     1681 atgcacctta tggaaaagat gaatcaccat ttttctttg
//
LOCUS       DAOQAN010000283         1717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_107129_length_1717_cov_1.803851, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000283 DAOQAN010000000
VERSION     DAOQAN010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107129_length_1717_cov_1.803851"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="PKO10_04985"
     CDS             <1..561
                     /locus_tag="PKO10_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HNW66533.1"
                     /translation="EELHFIVKIEDIKSEDYEALENRLVQILKDVYEQKMSKVGDKQK
                     SEIERILYLQILDNAYREHLYAMDTLKTGIGLRGYNQKDPLVEYKKESYNMFIDLVSN
                     IKLEIIKILFTIQLQSKEEQEALDKVKANMEKSNEHITTNLAQEAVKSSDKKIARNEV
                     CPCGSGLKYKQCCGKSGPKRGLVAGI"
     gene            563..>1717
                     /locus_tag="PKO10_04990"
     CDS             563..>1717
                     /locus_tag="PKO10_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNW66534.1"
                     /translation="MNKELINFIVKKYLKFDKKNPFISISAILAFIGVAIGVMVLILS
                     MAIMNGTAKEFERKLFTMNYPLTIYSKGANSVTEDLLLDLEANFKNLKFSPFISTQAI
                     VQNANNMSGGMIFGVLPQKEAKINPIFKEALRDFDFSKFDIITGVGISDKLLLTQNSK
                     LTLYFTELNPAGFSLMPKMKRFDYISSFNSGLSAYDKAYMYTTIEALQTLLQKDIGTY
                     DGIHVHSDDAFVDIEKLRTFLQDKRVGVVGWWQQNGNFFAAMKMEKTALFIVLMLIIL
                     VASLNIISSLLMTVMSRRKEIALLLSMGATAKEIKSIFLRVGTIIGFSGIITGVVLGF
                     IGYWLLDTFDIVTLPADVYGSAKLPLDLAMSDFISIIIGAVIIVLISSYYP"
BASE COUNT          637 a          204 c          295 g          581 t
ORIGIN      
        1 gaagaactac attttatagt aaaaatagaa gatataaaaa gtgaagatta tgaagcttta
       61 gaaaatagat tagttcaaat tttaaaagat gtttatgagc aaaaaatgag taaagttgga
      121 gataaacaaa aaagtgaaat tgaaagaatt ttatatcttc aaattttaga taatgcctac
      181 agagaacacc tttatgctat ggatactctt aaaactggaa ttggacttag aggatataac
      241 caaaaagatc ccctagttga atataagaaa gagtcttata atatgtttat agatttggtt
      301 tcaaatatta aattagaaat tataaaaatt ctttttacaa tacagcttca aagtaaagag
      361 gaacaagaag cactagataa agtaaaagct aatatggaaa aatcaaatga gcatataact
      421 acaaatcttg cacaagaagc tgtaaaaagt agcgataaga agatagctag aaatgaagtt
      481 tgtccttgcg gaagtggatt aaaatataaa cagtgttgtg gtaaaagtgg tccaaaaaga
      541 ggcttggtag caggaattta atttgaataa agaattaata aattttatag ttaaaaagta
      601 tctaaagttt gataagaaaa atccatttat ctctattagt gctattttag cttttattgg
      661 tgttgcaatc ggagttatgg tactaatatt atcaatggca attatgaatg gaactgcaaa
      721 agagtttgag cgaaaacttt ttactatgaa ttacccatta actatatatt caaaaggtgc
      781 aaatagtgta actgaagatt tacttttaga tcttgaggca aattttaaga atcttaaatt
      841 ttcaccattt atatcaactc aagctattgt tcaaaatgca aacaatatga gtggtggaat
      901 gatttttgga gtacttcccc aaaaagaggc aaaaattaac cctattttca aagaggcttt
      961 aagagatttt gattttagta aatttgacat tataacaggt gttggaataa gcgataaact
     1021 tcttcttact caaaatagta aattaacgct ctattttaca gagttaaatc cagccggttt
     1081 ttcacttatg ccaaaaatga aaagatttga ttatatttca tcttttaact ctggtcttag
     1141 tgcttatgac aaagcctata tgtacacaac tattgaagct ttacaaactc ttctgcaaaa
     1201 agatattggc acttacgatg gtattcatgt acattcagac gatgcttttg ttgatattga
     1261 aaagctaaga acttttttac aagataaaag agttggtgtt gttggttggt ggcaacaaaa
     1321 tggaaacttt tttgctgcca tgaaaatgga aaaaacggct ctttttatag tattaatgtt
     1381 gattatttta gttgcttcat taaatataat ttcatcttta cttatgaccg taatgagtag
     1441 aagaaaagag attgccctac ttttatcaat gggagcaacg gctaaagaaa ttaaatcaat
     1501 attcctaaga gttggaacaa taattggttt ttcaggaatt ataactggag ttgttttagg
     1561 atttatcggt tattggttat tagatacttt tgatattgtt actcttcctg ctgatgttta
     1621 tggaagtgcg aaactgccat tagatttagc aatgagcgat tttatttcta taattattgg
     1681 agctgtaatc atagttctta tttcatctta ttaccca
//
LOCUS       DAOQAN010000284         1712 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_107540_length_1712_cov_3.540736, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000284 DAOQAN010000000
VERSION     DAOQAN010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107540_length_1712_cov_3.540736"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            131..397
                     /locus_tag="PKO10_04995"
     CDS             131..397
                     /locus_tag="PKO10_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66535.1"
                     /translation="MRIVALILLLLFSSNHLFADSIESVLKIKPPIKYNQNQKYDKIK
                     KYGDNQSDGDNKKYDFGIDIGIDKELMTIDRLKIDVGTKFKGIN"
     gene            398..1078
                     /gene="hisIE"
                     /locus_tag="PKO10_05000"
     CDS             398..1078
                     /gene="hisIE"
                     /locus_tag="PKO10_05000"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HNW66536.1"
                     /translation="MKNIEKIDWAKMGDLIPVITQDSSSNEVLMLGYTNKEALEITLK
                     TGYAHYFSRSKQRIWKKGESSNHTQKIVNIFLDCDNDTLLFKVIQEGVACHTGRKSCF
                     FTNLENSEVVEDVKVDLSKTYSVIDNLYHTIQKRKNEDITKSYTAKLLNGSQNSMLKK
                     IVEEAGEFCFAIKDKNEDEAIYEAADITYHVLVALASLNINPDRVKQELARRFEISGI
                     EEKNSRNR"
     gene            1166..1423
                     /locus_tag="PKO10_05005"
     CDS             1166..1423
                     /locus_tag="PKO10_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66537.1"
                     /translation="MTNSCQHCSKKIPISKVFCSPACKENYFQKIAISVPKPFVKKLY
                     FFCTEEEKSYEIKTFAKRHNWHEELVIEKVEELFQEYYKCG"
     gene            complement(1467..>1712)
                     /locus_tag="PKO10_05010"
     CDS             complement(1467..>1712)
                     /locus_tag="PKO10_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006655603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HNW66538.1"
                     /translation="KELFDNGAYTVAQNEESCVVFGMPMKAIQAGAVKDIVHLDEIAD
                     YIIDFSKGRRSFSSYIFRVLVFVFRSSEILLSGKMFF"
BASE COUNT          725 a          222 c          254 g          511 t
ORIGIN      
        1 agaacaaaga tgcaaagatt ttcttaacac caggtggttc aactcctaat atttgggttg
       61 atacaaaaga taaaaataga gatactgtag ttaatcaaaa atagtatgta aattttaaaa
      121 gaggtggaga atgagaatag tagctttaat tttattatta ctattttcta gtaaccacct
      181 ttttgctgat tcaatagaat ctgttcttaa aataaaacct ccaataaaat ataatcaaaa
      241 ccaaaaatat gacaaaatca aaaagtatgg tgacaatcaa agtgatggtg ataacaaaaa
      301 atatgacttt ggaattgaca ttggtataga taaagagctt atgacaatag ataggttaaa
      361 aattgatgtt ggaacaaaat ttaaaggtat taattagatg aaaaatatag aaaagataga
      421 ttgggcgaaa atgggagatt taatacctgt tataactcaa gatagtagtt caaatgaagt
      481 tttaatgcta ggatatacaa acaaagaagc tttagaaatt acactaaaaa ctggatatgc
      541 acactacttt agtagaagta agcaaagaat ttggaaaaaa ggtgaaagct caaatcacac
      601 tcaaaaaata gtaaatattt tccttgattg cgataacgac actctattat ttaaagttat
      661 tcaagaagga gttgcttgtc atacaggaag aaaatcttgt ttttttacaa atctagaaaa
      721 tagtgaagtt gttgaagatg taaaagttga tttatcaaaa acatatagcg taatagataa
      781 tttataccac actatacaaa aacgaaaaaa tgaagatatt acaaaatctt acactgcaaa
      841 acttttaaat ggtagccaaa actctatgct taaaaaaatt gtagaagaag ctggagagtt
      901 ttgttttgca ataaaagata aaaacgaaga tgaagcaata tacgaagcag ccgatattac
      961 atatcatgtt ttagtagctc ttgcaagtct taatataaat cctgatagag taaaacaaga
     1021 gttagcaaga agatttgaaa tttcggggat agaagaaaaa aactcaagaa acagataaaa
     1081 attattaata tttcttatac aactaagttg catttaataa ttgcttgtta taatgctcgc
     1141 aacaaaaact aaaaaaggag tttaaatgac aaatagctgt caacactgct ctaagaaaat
     1201 accaatttca aaagtttttt gctctccagc ttgcaaagag aactattttc aaaaaattgc
     1261 aatatcagtt ccaaaacctt ttgtaaaaaa attatacttt ttttgtacag aagaggagaa
     1321 aagctatgaa atcaaaactt ttgctaaaag acacaattgg catgaagagt tagtaataga
     1381 aaaagtagaa gaattattcc aagagtatta caagtgtgga taaattccat acttgtttaa
     1441 aaataaaaaa tggcactttt aaataatcaa aaaaacatct taccacttag tagtatttca
     1501 gaacttctaa atacaaaaac aagaactcta aaaatgtatg aagagaaact tcttcttcct
     1561 ttagaaaaat caattatata atcagctatt tcatctaaat gaactatatc ttttactgca
     1621 cctgcttgta ttgctttcat aggcattcca aaaacaacac aactctcttc attttgtgca
     1681 actgtatatg ccccattatc aaaaagttct tt
//
LOCUS       DAOQAN010000285         1709 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_107841_length_1709_cov_3.044740, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000285 DAOQAN010000000
VERSION     DAOQAN010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107841_length_1709_cov_3.044740"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="PKO10_05015"
     CDS             complement(<1..279)
                     /locus_tag="PKO10_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66539.1"
                     /translation="MKKLRLLASLAFLILIISGCANVLDNLTTKTSPKVNQNFQSIDY
                     SSIKSIPDMNSIGFEWQRIDDHRVVGYNFYRTELSKGQNTLKLIKATDS"
     gene            complement(227..1213)
                     /locus_tag="PKO10_05020"
     CDS             complement(227..1213)
                     /locus_tag="PKO10_05020"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013135207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNW66540.1"
                     /translation="MYKNFIVDVEIRLDKFLSLKIDASRNQIEQLIQKEFVKVDGKTT
                     DKNGLKLKLNQEIEVFFPEPKTFDKKNSEFIKESLKDTNIEIIYEDSDILIINKPRGL
                     TVHDAPSVKDATLVDWLKLQNISLSTISGDERHGIVHRLDKGTSGILIVAKNNEAHIE
                     LSKQLEKREAGRYYLALIDLPLKDNIIVEEPIGRNPNNRLKMSIQKDGRYAKTSFCKL
                     EVSNNSKYELIACKLSTGRTHQIRVHLNRLNRHILGDILYGFKGDFSIINGFFLHGFC
                     LVFTHPRTKEKMSFTAKLPKDMQDFIDTNFDKERIYEKIETSSLFSFFNFNN"
     gene            1257..>1709
                     /locus_tag="PKO10_05025"
     CDS             1257..>1709
                     /locus_tag="PKO10_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HNW66541.1"
                     /translation="MSHFDYLILIFIAPLIILSYVLISEANDALASKQVFYYTISLFI
                     FILVFMFPLRKNLRLVPFLYWIGILLLLAVEFIGVTKLGAKRWIHIPLLDTTIQPSEI
                     IKPIYILMLGYLIQRKPPPIGGYGLKDFGYFSIYIFIPFFLIAKEPDLG"
BASE COUNT          546 a          261 c          201 g          701 t
ORIGIN      
        1 actatctgtt gcttttatta acttcagagt attttgacct tttgataact ctgttctata
       61 aaaattataa ccaacaactc tatggtcatc tattctttgc cactcaaaac caattgaatt
      121 catatctgga attgatttaa ttgaagagta atctattgac tgaaaatttt gattaacctt
      181 tggtgaagtt tttgtagtaa ggttatctaa aacatttgca caaccactaa ttattaaaat
      241 taaaaaagct aaagaggcta gaagtctcaa ttttttcata tattctctcc ttgtcaaaat
      301 tggtatctat aaaatcttgc atatctttag gaagttttgc agtaaaactc atcttctctt
      361 tagttctagg gtgagtaaat actaagcaaa atccatgcaa aaaaaatcca ttgataatac
      421 taaaatcgcc cttaaatcca tataaaatat cccctaaaat gtgcctattc aatctattta
      481 ggtgaactct tatttgatgt gttcttcctg ttgaaagttt acaagctatt agttcatatt
      541 ttgagttatt actaacttct agcttacaaa atgatgtttt tgcatatctt ccatcttttt
      601 gtatagacat tttaagtcta ttatttgggt ttcttcctat tggttcttct acaataatat
      661 tatcttttaa aggtaaatca atcaaagcta aatagtatct tccagcttct cttttttcta
      721 gctgtttaga tagttcaata tgagcttcat tattttttgc aactattaaa atcccactag
      781 ttcctttatc aagtctatgt acaattccat gtctttcatc accacttata gtcgataaac
      841 ttatattttg aagctttagc caatcaacta aagttgcatc ttttacgcta ggagcatcat
      901 gaacggtaag accacgaggt ttatttataa ttaaaatatc actatcttca tatattatct
      961 ctatatttgt atcttttaat gactctttta taaattcact atttttttta tcaaatgttt
     1021 ttggttctgg aaaaaatacc tctatttctt gatttaattt tagttttaaa ccatttttat
     1081 ctgttgtttt accatctact tttacaaact ctttttgaat tagttgctca atttgatttc
     1141 ttgaagcatc tatttttaaa cttaaaaatt tatctaatct aatttctaca tctacaataa
     1201 aatttttata catattatgc tactctttcg ctatgcgttt acttgataaa agaattatgt
     1261 cacattttga ttatttaata ttaatcttta tagccccttt aataatacta tcttatgtat
     1321 taatatcaga ggcaaatgat gcattggcaa gtaaacaagt attttattat actatatcct
     1381 tatttatatt tattttagtt tttatgtttc ctcttagaaa aaatctaaga ttagttcctt
     1441 ttttatattg gataggaatt ttgcttttac tagcagtaga atttataggg gtaacaaaac
     1501 taggtgcaaa aagatggata catattccac ttcttgatac aactattcaa ccttctgaga
     1561 ttataaagcc gatatatata ttgatgttag gatatttaat acaaagaaaa ccaccaccta
     1621 ttggtggata tggattaaaa gattttggat acttttcgat ttatattttt attccatttt
     1681 ttctaatagc taaagagcca gatttggga
//
LOCUS       DAOQAN010000286         1707 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_108056_length_1707_cov_2.680387, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000286 DAOQAN010000000
VERSION     DAOQAN010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108056_length_1707_cov_2.680387"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="PKO10_05030"
     CDS             complement(<1..238)
                     /locus_tag="PKO10_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66542.1"
                     /translation="MKIRASLLTIVLFSFIFSGCATTSTSQNNLSTPDNKYSYDGKLS
                     QEQKHLKNHNFIDDITYSKYMSKRDMNINNELFSF"
     gene            complement(321..977)
                     /locus_tag="PKO10_05035"
     CDS             complement(321..977)
                     /locus_tag="PKO10_05035"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HNW66543.1"
                     /translation="MKSKLLITFFIGIFFVTSSADEVKKSEEWKIQNDPYYKHKVSQF
                     EMLKDRNNIEIMMLGDSITDEGEWSELWGKVVQNRGISGDTTSGVLDRLYTLNPNTKR
                     VFIMIGVNDIMRGVSEDIVFENYKKILKFFQDKNIEVIIQSTLYIGESRKQNFNVKIE
                     KLNQNLEEFAKSNKIVFIDLNPIFAPQKTLLKSFTKDDLHLNADAYKLWIETLKKLKY
                     "
     gene            1105..>1707
                     /locus_tag="PKO10_05040"
     CDS             1105..>1707
                     /locus_tag="PKO10_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012148043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HNW66544.1"
                     /translation="MSSRTIKLLAPVLVVLVMWFIPAPEGLSQNAWHFLAIFLAVVIG
                     LILEPVPAALVGFTGISVVALLGLIGNSKESVNWALSGFSNSVIWLIFAAFMFAAGYK
                     KTGLGRRVSLIMVKYMGKSTLGLGYAVAFSDLVLAPFMPSNTARSGGSIYPVAINIPQ
                     IFNSLPDNEPRKIGAYISWVAIASTCVTSSMFLTALAPNLL"
BASE COUNT          549 a          254 c          214 g          690 t
ORIGIN      
        1 aaaaactaaa tagctcatta tttatattca tatctctttt tgacatatat tttgaatatg
       61 taatatcatc aataaaatta tgatttttta aatgcttttg ttcttgactt aatttaccat
      121 catatgaata tttattatct ggagtagaga gattattttg acttgtagag gttgttgcac
      181 aaccagagaa aatgaaacta aataaaacaa ttgtaagtaa actagccctt attttcatac
      241 tttttccctt ttgtcaaaat aaaatgtttt tgattgtaca aaaaaacaaa tcaaattgtc
      301 aagcaatatt gattgttttt ttaatattta agcttcttta aagtttctat ccataatttg
      361 tatgcatctg catttaaatg taaatcatct tttgtgaaac tctttaaaag agttttttgt
      421 ggtgcaaaaa ttggatttaa atctataaaa acaattttat tactttttgc aaactcttct
      481 aaattttgat ttagtttttc aatttttaca ttaaaatttt gttttctact ctctcctata
      541 tataatgtag attgaataat aacttctata tttttatctt gaaagaattt taaaattttc
      601 ttataatttt caaaaactat atcttcacta actcctctca taatatcatt tacaccaatc
      661 attataaaga ctcttttagt atttggattt aaagtataaa gtctatctaa gaccccactt
      721 gttgtatctc cactaatacc tctattttga actacctttc cccaaagttc actccactcc
      781 ccttcatcag taatagaatc acccaacatc ataatttcaa tattgtttct atcttttaac
      841 atctcaaatt gactaacttt gtgtttataa taagggtcat tttgaatttt ccactcttca
      901 gattttttta cttcatctgc actagatgta acaaaaaaga ttcctataaa aaatgtaatt
      961 aataacttac ttttcaatat aactcctaat tttttaataa agtatagcaa atattctatt
     1021 ataaaatatt atttaaatta atcttataat ctatatgtaa taaatttata ttataatttc
     1081 ttaatttttt tattaggagg ttttatgtct tcacgaacta taaaactact agctcctgtg
     1141 ttagtagtat tggttatgtg gtttattcca gcaccagagg gcttatcaca aaatgcttgg
     1201 cattttttgg cgatattttt agcagtcgta attggtctta ttcttgaacc tgttcctgca
     1261 gctcttgtag gatttactgg tatttcagta gtagctcttt taggattaat tggaaattct
     1321 aaagagagtg taaactgggc attaagtggt ttttcaaata gtgttatttg gttaattttt
     1381 gctgcattta tgtttgctgc tggttataaa aaaacaggtc ttggaagaag agtatcattg
     1441 attatggtta aatatatggg gaaaagcaca ttaggcttag gttatgccgt tgcattttct
     1501 gatttagttc tagctccatt tatgccatca aatactgcaa gaagtggagg aagtatctat
     1561 cccgtagcta ttaatattcc acaaattttc aactctcttc cagataatga gccacgtaaa
     1621 attggtgcat atatctcttg ggttgcaata gcatcaacat gcgtaacaag ttctatgttt
     1681 ttaactgctc ttgcaccaaa tttacta
//
LOCUS       DAOQAN010000287         1706 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_108148_length_1706_cov_2.682617, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000287 DAOQAN010000000
VERSION     DAOQAN010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108148_length_1706_cov_2.682617"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..1668)
                     /locus_tag="PKO10_05045"
     CDS             complement(79..1668)
                     /locus_tag="PKO10_05045"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNW66545.1"
                     /translation="MNIKLPKSSTLLFVNILIFLFITLFAYFILQENIKLNNSKNQEI
                     LFFKIKDKSSALLTKVLQKYHNKKEFIKERHQIALTLLEKGLDVDSIKINLNKDLEYN
                     KFEVSILSKDLIIEDSSIFTDIGTDLSLLKKQFKKFESSQEIKVSIPEYSLEFLKFLS
                     YSTSSLKNGKYLQISYSYDEFISELREIQEFINNTPIIEKSISYIISDDYVGNFAFKT
                     IPSHKKTIEELESRLKKGSELLGVLGGNSYISYYKTVDNKKLHIAYLLQNSPIYTDAE
                     ILFCIVFDENQFIRDIMYLKLVSFFVFIAGATAIYLTYKLRTKELLLNYKDKFIAHSI
                     HEIKTPLSIITINIQLREKLYGEDKYTKKIDGALKTLENSYEDMTFLHTKDKIEYEIV
                     EINLQKALENRVKYFSTIADCQNRKIELIANNNFYPKMSKIELNRLVDNNISNAIKYS
                     NIGSTISIILKDNILEFHSKGAKIENPKGIFKKYKREDKNTGGHGLGLAIVSDICKKY
                     NFEIEVLSQNSINTFRYILKN"
BASE COUNT          580 a          215 c          177 g          734 t
ORIGIN      
        1 catatctttt attgattcat ttagtgaaaa atcatcttca agcaaaaaaa tcttcataat
       61 aattccccct aaaaattatt aattctttag aatatatcta aaagtattta tagagttttg
      121 acttaaaacc tctatttcga aattatattt tttacaaata tcactaacta ttgctaatcc
      181 cagaccatgt ccacctgtat ttttgtcttc tcttttatat tttttaaaga ttccttttgg
      241 attttctatt ttagcacctt ttgagtgaaa ttcaaggata ttatctttta aaattataga
      301 tatagtggaa ccaatgtttg aatattttat agcatttgag atattgttat caaccaatct
      361 gtttagttct attttactca tttttggata aaaattattg ttagctataa gttctatttt
      421 tctattttga caatctgcaa ttgtactaaa atactttaca cgattttcta gcgctttttg
      481 aagatttatt tcaacaattt cgtactcaat tttatctttt gtatgaagaa aagtcatatc
      541 ttcatatgaa ttttctaaag tttttaaagc tccatctatc ttttttgtat atttgtcttc
      601 accataaagt ttctctctta gttgaatatt tattgtaata attgatagtg gagtttttat
      661 ttcatgaata gagtgtgcaa taaatttatc tttatagttt aaaagtagct cttttgttct
      721 tagtttataa gtaagatata tagcagttgc tccagcaata aaaacaaaaa acgaaactaa
      781 ctttagatac ataatatctc ttataaattg attctcatca aaaacaatac aaaataaaat
      841 ttctgcatcg gtataaattg gactattttg aagtaaataa gctatatgaa gtttcttatt
      901 gtcaactgtt ttatagtatg aaatataact atttcctcct aaaaccccta aaagttcact
      961 tccttttttt aatctacttt ctaactcttc aattgttttt ttatgtgaag gtatagtttt
     1021 gaaagcaaaa tttccaacat aatcatcaga tataatgtat gatatagatt tttcaataat
     1081 aggagtatta tttataaact cttgaatctc tcttagttcg ctaataaatt catcataaga
     1141 gtatgatatt tgcaaatatt taccattttt taaactagaa gttgaataac ttaaaaattt
     1201 taaaaactct aaagagtatt ctggaattga tacttttatt tcttgactgc tttcaaattt
     1261 tttaaactgt tttttaagta atgataggtc ggttcctata tctgtaaaaa ttgaactatc
     1321 ttcaattatt aaatctttac ttaatataga aacttcaaat ttattatatt ctaaatcttt
     1381 atttaaattt atttttatac tatcgacatc taatcctttt tctaaaagag ttaaagctat
     1441 ttggtgtctc tcttttataa actctttttt attatgatac ttttgcaaaa cttttgttaa
     1501 aagagcagag cttttatctt ttattttgaa aaaaagtatc tcttgatttt ttgagttatt
     1561 taattttata ttttcttgta gtataaaata tgcaaatagt gtaataaata agaaaattaa
     1621 tatatttaca aataataaag ttgatgattt tggtagtttt atattcaatt ttactcctaa
     1681 aagataattc tactcaaaat tagttt
//
LOCUS       DAOQAN010000288         1705 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_108290_length_1705_cov_1.773333, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000288 DAOQAN010000000
VERSION     DAOQAN010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108290_length_1705_cov_1.773333"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1705)
                     /locus_tag="PKO10_05050"
     CDS             complement(<1..>1705)
                     /locus_tag="PKO10_05050"
                     /inference="COORDINATES: protein motif:HMM:NF026177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNW66546.1"
                     /translation="TLVSISYYFYYSPKKEEITKEIFLEKSIQMRKLFIDEIKRKQDN
                     ALNMAFILSQDENLINALKTKNKSLLNYNNTLLFLHDNSDYKNIWLHIVDKNGRSFYR
                     SWKNMSGDDLSNVRTDLDELIKNPKPITNISSGLFDLTLKAINPIYDTNGQFLGFVEF
                     ISKFNSISKNLKFEKIEPIFILSREKSKKLIEPFSKIFIRDNYIANIDANQSILKYIE
                     RIGVDKFLNIENYLLVDKYIVTNLEIKDTNGQDMGLFLLFFEKNRLDYSSLINFKNQY
                     LYIVIIFSILYLIVFLYLLKTMYAKELDNDVKIKTKMIQEQKNKLKKLLDIYDKNVIF
                     SRTDLNGIITHASSAFCKISGYTKDELLGQPHSIVRHPDMPKSTFKKIWDSLKAKEKI
                     TVEIKNLKKDGSYYWVVADFEPEYDDLGNHIGYFAIREDITANKEIEELQKEVIFTMG
                     SIAEFRSKETGEHIKRVAKYSRILAAAYGLCEDDIDMLELASPMHDIGKIAIPDAILN
                     KPGKLTNEEFEIIKTHTQKGHDMLGISNRPLFKVASQIALTHHEKYDGTGYPNSLKGD
                     DI"
BASE COUNT          555 a          244 c          187 g          719 t
ORIGIN      
        1 tatatcgtca ccttttaaac tatttggata tcctgttcca tcatattttt catgatgagt
       61 taaagctatt tgtgaagcaa ctttaaaaag tggtctattt gatattccaa gcatatcatg
      121 acctttttgt gtatgagttt taataatttc aaactcttca tttgttaatt tccctggttt
      181 atttaaaata gcatcaggaa ttgcaatttt accaatatca tgcataggac ttgcaagctc
      241 taacatatca atatcatctt cacataaacc ataagcagct gctaaaattc ttgagtattt
      301 tgcaactctt ttaatgtgtt ctccagtttc tttggatcta aactcagcaa tagagcccat
      361 agtaaagata acttcttttt gaagttcttc tatctcttta tttgcagtaa tatcttctct
      421 aatagcaaaa tatcctatat gatttcctaa gtcatcatat tcaggttcaa aatcggcaac
      481 tacccaatag tatgaaccat ctttttttaa gttttttatc tcaacagtta ttttttcttt
      541 tgcttttaaa ctatcccaaa tctttttaaa tgtagatttt ggcatatcag gatgccttac
      601 aatactgtgt ggttgtccca aaagttcatc ttttgtataa ccactaattt tacaaaaggc
      661 gctacttgca tgagttatta ttccatttaa atcagttctt gagaatatta catttttatc
      721 ataaatatca agtagttttt taagtttgtt tttttgttct tgaatcattt ttgtcttaat
      781 ttttacatca ttatcaagct cttttgcata catagttttt agaagatata aaaatactat
      841 aagatataaa attgagaata ttataacaat atataaatat tgatttttaa aatttattaa
      901 agatgaataa tctaatctgt ttttttcaaa aaatagtaaa aatagaccca tatcttgacc
      961 atttgtatct tttatctcta aatttgtaac aatatattta tcgactaata gatagttttc
     1021 aatatttaaa aatttatcaa cacctattct ttcaatatat tttaaaatag attggtttgc
     1081 atctatattt gctatataat tatcacgtat gaatatttta ctaaatggct caattagttt
     1141 tttacttttt tcacgactta aaataaaaat gggctctatt ttttcaaatt ttaagttttt
     1201 tgatatagag ttaaattttg aaataaactc tacaaaacct aaaaattgac catttgtatc
     1261 ataaattgga tttatagctt ttaaagttaa gtcaaacagt cctgagctta tatttgtaat
     1321 tggttttgga ttttttataa gttcatctaa atctgttcta acatttgata aatcatcacc
     1381 tgacatattt ttccaagatc tataaaagct ccttccattt ttatcaacaa tatgcaacca
     1441 aatatttttg taatcactgt tatcatgtaa aaataataga gtattattat aatttagtaa
     1501 gcttttattt tttgttttta aagcatttat aagattctcg tcttgactta agataaatgc
     1561 catattaagc gcattatcct gttttctttt tatttcatct ataaaaagtt ttctcatttg
     1621 aatacttttt tctaaaaata tctcttttgt tatctcctct ttttttggag agtagtaaaa
     1681 atagtatgat attgagacta gagtt
//
LOCUS       DAOQAN010000289         1704 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_108332_length_1704_cov_3.101880, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000289 DAOQAN010000000
VERSION     DAOQAN010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108332_length_1704_cov_3.101880"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="PKO10_05055"
     CDS             <1..212
                     /locus_tag="PKO10_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNW66547.1"
                     /translation="LCMVAKGVYEGYYEMNLKPWDVSAGVIILSEAGGRITNIRGTTF
                     DMFKDKYIVASNGKIHDKLLSKLDI"
     gene            265..>1704
                     /gene="glmS"
                     /locus_tag="PKO10_05060"
     CDS             265..>1704
                     /gene="glmS"
                     /locus_tag="PKO10_05060"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HNW66548.1"
                     /translation="MCGIVGYIGKYDTTKILLDGLKELEYRGYDSAGIAVLHGNEIDV
                     FKAVGKLVNLEEKVNQNNKDRYNLGIGHTRWATHGKPTEVNAHPHLGEYSYVVHNGII
                     ENYKELKDELTSLGHNFVSQTDTEVIVHLFEHYNNKLNDAKKAFQETIKRLEGAYAIL
                     LITKKDPEKIFFYKLGSPMIVGHGIEKDEVLFASSDSALIGLANDVVYLDDKIGGVAS
                     RDGIEFFSKNIVWSKLPTSKQFAQKDGFRYFMEKEIYEQSVVVSDCMLGRVKDSEINF
                     DEIDSKILDGINEIKICACGTSYHAGLASSYLFERLSKIKCSVEVASEFRYKEPLLTK
                     DTLFVVISQSGETADTLEALKMAKSAGLKTLVICNVDNSSMTRVADFTILTRAGIEKG
                     VASTKAFSTQTVVLWMLSLYFAQIKETISKEKLENEVNTLREVPKALKVHENIHEKTK
                     RLSKRYLHGHGFFFIGRDVFYPLALEGALK"
BASE COUNT          644 a          174 c          326 g          560 t
ORIGIN      
        1 atttatgtat ggttgcaaaa ggcgtttatg aaggttacta tgaaatgaat ctgaaacctt
       61 gggatgttag tgctggagtt ataattttaa gtgaagctgg tggaagaatt acaaatatta
      121 gaggtactac ttttgatatg tttaaagata aatatatagt tgcctcaaat ggtaaaattc
      181 atgataaatt gctaagtaaa ctagatattt agttagtttt tggtaaaatc ttatattttt
      241 taaaatgata tttaaggact ttttatgtgt ggaatagttg gttacatagg gaaatacgat
      301 acaacaaaga ttttattaga tggtttaaaa gaacttgaat atagaggata tgatagcgca
      361 ggaattgctg ttttacatgg aaatgaaata gatgtattca aagcagttgg gaagcttgta
      421 aatcttgagg aaaaagttaa tcaaaataat aaagatagat ataatttagg aattggacat
      481 acaagatggg caactcatgg aaaaccaaca gaagttaatg ctcatccaca cttaggtgaa
      541 tactcttatg ttgttcataa tgggataata gaaaattata aagagttaaa agatgagtta
      601 acatctttag gacacaattt tgtttcacaa acagatacag aagttattgt tcatcttttt
      661 gagcactaca acaataaatt aaatgatgca aaaaaagcat ttcaagagac tataaaaaga
      721 cttgaagggg cttatgctat tttattaatt acaaaaaaag atccagaaaa aatatttttc
      781 tataaacttg gaagtccaat gattgttgga catggtatag aaaaagatga agtacttttt
      841 gcatcttcag attcagcact tattggactt gcaaatgatg ttgtatatct tgatgataaa
      901 attgggggag tagcttcaag agatgggatc gagtttttta gtaaaaatat tgtttggtca
      961 aaacttccaa catcaaaaca gtttgctcaa aaagatggtt ttagatactt tatggaaaaa
     1021 gagatttatg agcaaagtgt tgttgtaagt gattgtatgt taggtcgtgt aaaagatagt
     1081 gagataaatt ttgatgagat tgattcaaaa atattagatg gaataaatga gattaaaatt
     1141 tgtgcatgtg gaacttcata tcatgcaggg cttgcatctt catatctgtt tgaaagatta
     1201 tcaaaaataa aatgtagcgt tgaagttgca agtgagttta gatataaaga gccactattg
     1261 acaaaagata cactatttgt tgtaatctct caaagtggag aaacagctga tacattagaa
     1321 gctttaaaaa tggcaaaaag tgcaggtctt aaaactttgg taatttgtaa tgttgataac
     1381 tcttctatga caagagttgc agattttaca attttaacaa gagctggaat tgaaaaagga
     1441 gttgcttcta caaaagcatt ttctactcaa acagttgttt tatggatgtt aagtttatat
     1501 tttgcacaaa ttaaagaaac tatttctaag gaaaaacttg aaaatgaggt taatacttta
     1561 agagaggttc caaaagcttt aaaagttcat gaaaatattc atgaaaaaac aaaaagatta
     1621 tcaaaaagat atttacacgg tcatggattt ttctttatag gaagagatgt attttatccc
     1681 cttgctttag aaggtgcttt aaaa
//
LOCUS       DAOQAN010000290         1702 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_108564_length_1702_cov_2.534305, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000290 DAOQAN010000000
VERSION     DAOQAN010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108564_length_1702_cov_2.534305"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="PKO10_05065"
     CDS             <1..391
                     /locus_tag="PKO10_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014769178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HNW66549.1"
                     /translation="NIFFDGQIYDAYSFVNDLIKLANSEIVLIDNYIDDTVFTLFSKY
                     PNINFIIYTNNISKQLKLDFEKYSKQYKNIALKTFKSSHDRFLIIDKKDIYHLGASLK
                     DLGKKWFAFSKMSLDSLNLDEIFNRLK"
     gene            501..698
                     /locus_tag="PKO10_05070"
     CDS             501..698
                     /locus_tag="PKO10_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66550.1"
                     /translation="MLITIENADKKLLDVIKSIIKLSPNANLKIEKEPTNELLEAIEE
                     VKNGKVESFNDFETYKKAMEL"
     gene            698..973
                     /locus_tag="PKO10_05075"
     CDS             698..973
                     /locus_tag="PKO10_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018712586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HNW66551.1"
                     /translation="MLFEIITTLKFKKQRKKLKQDDKDLVDNIVFILANNQILDKKYK
                     DHQLKGNLKEFRECHVKPDLLLIYKKENNILVFTCIAVGSHSDLFKK"
     gene            1061..1258
                     /locus_tag="PKO10_05080"
     CDS             1061..1258
                     /locus_tag="PKO10_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66552.1"
                     /translation="MHTIKLKVHDDIYNHIIFLLNSLDKKKIEIVEEKPLIINKNKKR
                     LNAIKIDTTNFKFDRQEANER"
     gene            1248..1652
                     /locus_tag="PKO10_05085"
     CDS             1248..1652
                     /locus_tag="PKO10_05085"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNW66553.1"
                     /translation="MKDKIFIDTNILLYAYSTEKNKQEIAQNIINTNNNIYISKQVIN
                     ETINILIKKFKLNIKDIINVVKELEKEFIILDFDIQTQLNALKLKQNYNLQFYDALIV
                     STALKNSCTILYSEDMQDKLVIEKKLKIINPF"
BASE COUNT          758 a          148 c          197 g          599 t
ORIGIN      
        1 aaacatcttt tttgatggtc aaatttatga tgcttacagt tttgtaaatg acttaataaa
       61 actagcaaat agtgaaatag ttttgataga taattatata gatgatacag tttttacttt
      121 gtttagtaag tatccaaata taaattttat aatttatacg aacaatatct caaagcaatt
      181 aaaattagat tttgaaaaat attcaaaaca atataaaaat attgctctaa aaacttttaa
      241 atctagccat gatagatttt tgattataga taaaaaagat atttatcatt tgggagctag
      301 cttaaaagat ttgggtaaaa aatggtttgc cttttcaaag atgagtttgg actctttaaa
      361 tttggatgag atttttaata gattaaaata atcttgtgtc acgatttttt gttaattatg
      421 taaaaaaagt aaatataaac tttttgcctt tttacttcct aatttgatat aatcgtttaa
      481 aaaatttata aaggaataaa atgcttataa ctatagaaaa tgcagataaa aaacttttag
      541 atgttattaa aagtattata aaactttcac caaatgctaa tttaaaaatt gaaaaagaac
      601 caacaaatga gttattagaa gccattgaag aagttaaaaa tggaaaagtt gaatctttta
      661 atgattttga aacttataaa aaagcaatgg aactttaata ttgtttgaga taataaccac
      721 tctaaaattc aagaaacaaa gaaaaaaact aaaacaagat gataaagatt tagtggataa
      781 tatagttttt attttggcaa ataatcaaat tttagataaa aaatataaag accaccaact
      841 aaaaggaaat ttaaaagagt ttagagagtg tcatgtaaaa cctgatttac ttttaatata
      901 taaaaaagaa aataatattt tagtttttac ttgcatagct gttggttctc atagcgattt
      961 atttaaaaaa taataatacc taaaaaagta aatataaact ttttgccttt ttacttccta
     1021 atttgatata atcgtttaaa aaatttataa aggaaaatat atgcatacaa ttaagctaaa
     1081 agttcacgat gatatttata accatataat atttttattg aatagtttag ataaaaaaaa
     1141 gattgaaata gttgaagaga agcctttgat aataaataaa aataaaaaaa gattaaatgc
     1201 aataaaaata gacacaacta attttaagtt tgatagacaa gaagcaaatg aaagataaaa
     1261 tttttattga tacaaatata cttttatatg cttattcaac tgaaaaaaat aaacaagaaa
     1321 tagcacaaaa tattataaat acaaataata atatttatat ttcaaaacaa gttataaatg
     1381 aaacaattaa tattctaata aaaaaattta aattaaatat aaaagatatt ataaatgtag
     1441 taaaagaact tgaaaaagag ttcattattt tagattttga tatacaaaca caattaaatg
     1501 ctttaaaact taaacaaaat tataacttac aattttatga tgctttgatt gtatcaacag
     1561 ctttaaaaaa ttcttgtact attttatata gtgaagatat gcaagataaa ttggtaattg
     1621 aaaaaaaatt aaaaattatt aatccttttt aatattaaaa tattgacagc aaggttgggc
     1681 ttttgctcaa tctttttttt tg
//
LOCUS       DAOQAN010000291         1701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_108656_length_1701_cov_2.818348, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000291 DAOQAN010000000
VERSION     DAOQAN010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108656_length_1701_cov_2.818348"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(33..764)
                     /gene="fliP"
                     /locus_tag="PKO10_05090"
     CDS             complement(33..764)
                     /gene="fliP"
                     /locus_tag="PKO10_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012711.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HNW66554.1"
                     /translation="MKIIFSLVFSSLFLFAAQDPLPMINLSVAAVDQPIQFVKTINIA
                     VILALMVLAPSLLLMVTSFTRIIIVLALLRQALGLQQSPPTQIIISLALIMTIFIMEP
                     YGKKAWDESIVPYMDEKISYQEAFTKGVAPFKDFMIKNTRESDLALFYRIKKEPNPKN
                     IEDVSLILLMPAFIVSELRTAFEIGFLIFLPFLIIDIIVASILMSLGMMMLPPVMISI
                     PFKILLFVLVDGWGLVIGSLVKSFG"
     gene            complement(817..>1701)
                     /locus_tag="PKO10_05095"
     CDS             complement(817..>1701)
                     /locus_tag="PKO10_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNW66555.1"
                     /translation="HPFTNKIINGLYPPGSVIKMGVALSFLENGISDKFTVQCSGSLP
                     IGNRNFRCWKTTGHGHIGFRSSIEQSCDDFYYKGSLKIGIGKMSATLAKLGIGEKTGI
                     DLHNEFIGINPNKEWKEQKLKKPWYIGETVISSIGQGNMLTTPLQIARFTAFFASGKL
                     PKPHLTKDAYEEPKELDFKKEHIKLMQEGMFDVANSPSGTAKRYINSKVKIAAKTGTA
                     QVVSIPQSEKVRMKESEMEYFQRSHAWITTYGPYKNPKYVVTIIQEHGGGGGSATGGI
                     ASKIYDKLYELGYITELE"
BASE COUNT          570 a          296 c          213 g          622 t
ORIGIN      
        1 aatttcgcaa ttacctttct taaacaaatt tattatccaa aacttttgac aagggagcct
       61 ataaccagcc cccaaccatc cacgaggaca aaaagcagaa ttttgaacgg gatagatatc
      121 ataacaggtg gcaacatcat catacccaaa ctcatcaaaa ttgaagcaac tataatatct
      181 ataattaaaa atggtaagaa tatcaaaaat cctatctcaa atgcagttct tagttcactt
      241 actataaatg caggcataag taaaataagt gaaacatctt ctatattttt tggattaggt
      301 tcttttttta ttctataaaa tagtgctaaa tctgattctc tagtattttt tatcataaaa
      361 tctttaaaag gagccacacc ttttgtaaaa gcttcttgat atgatatttt ttcatccata
      421 taaggcacaa tactctcatc ccaagctttt ttcccataag gttccataat aaaaattgtc
      481 ataataagtg caagagaaat tattatttgt gtaggtgggg attgttgtaa acccaaagct
      541 tgtcttaaaa gtgctaaaac aattataatt cttgtaaaag atgtcaccat aagtaaaagc
      601 gatggagcaa gaaccataag agctaaaata acagctatat ttatagtttt tacaaattgt
      661 attggttgat ctaccgctgc aactgaaaga tttatcattg gtaatggatc ttgagccgca
      721 aataaaaata gtgatgaaaa aactagtgaa aaaattatct tcaaatataa atcctaaatt
      781 aaatggataa tattttatct aagatatgat taaatattac tctaattctg ttatatatcc
      841 caattcatat aatttatcat aaattttact agcaattcca cctgtggcac ttccaccacc
      901 accaccatgt tcttgtatta ttgttactac atattttgga tttttatatg gtccataagt
      961 agtaatccaa gcatgagatc tttgaaaata ctccatttca ctctctttca ttctcacttt
     1021 ttcactttgt ggaatagata caacttgagc agttcctgtt tttgctgcta tttttacttt
     1081 tgagtttata tatctttttg cagttcctga tggagagttt gcaacatcaa acattccttc
     1141 ttgcataagt tttatatgct cttttttaaa atctaactct tttggttctt cataagcatc
     1201 ttttgttagg tgtggttttg gaagttttcc acttgcaaaa aatgctgtaa atcttgctat
     1261 ttgtaaagga gttgtcagca tatttccttg accaatagaa ctaataacag tttcaccaat
     1321 ataccaaggt ttttttaatt tttgttcttt ccactcttta tttggattaa tacctataaa
     1381 ttcattgtgt aaatctatac cagttttttc tccaattcct aattttgcca aagttgcaga
     1441 catctttcca attcctattt ttaaactccc tttataataa aaatcatcac aactttgttc
     1501 aattgaactt ctaaatccaa tatgcccatg acctgttgtt ttccaacacc taaaatttct
     1561 atttccaatt ggtaaagaac cactacattg aactgtaaat ttatcactta ttccattttc
     1621 taaaaatgat agtgcaactc ccatttttat aacagaacca ggaggatata atccatttat
     1681 aatcttattt gtaaaaggat g
//
LOCUS       DAOQAN010000292         1701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_108688_length_1701_cov_2.244228, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000292 DAOQAN010000000
VERSION     DAOQAN010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108688_length_1701_cov_2.244228"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /locus_tag="PKO10_05100"
     CDS             complement(<1..714)
                     /locus_tag="PKO10_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNW66556.1"
                     /translation="MKIANRMEKLAPSLTLSITALANELKAAGKDILSFSAGEPDFDT
                     PQVVKDAAIKAINEGKTKYTAVEGIKETKQAIINKLKKDHNLDYTLDKIVISNGAKHS
                     LFNLAQALIDEGDEVIIPSPYWVTYPELVVYSGGVPVFIETDESTEFKITAKQLKEKI
                     TPKTKILMLNSPSNPTGSIYSRDELLAIGEVLKGTDIVVLSDEMYEKLIYKGKKFTAT
                     AEVSEDMYNRTVTINGLSKS"
     gene            797..1390
                     /locus_tag="PKO10_05105"
     CDS             797..1390
                     /locus_tag="PKO10_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF45 domain-containing protein"
                     /protein_id="HNW66557.1"
                     /translation="MNFEINLNNSVISVELHNKKHIKHCYLRILRKDLLQIKANRYFT
                     IYDAKDLIDRKKDWILENIKRVESKTLEDGYFLYLGEKKLLSDFAIKNLDSFYKKEID
                     SFISTFIEKYSNLMQLFPTKISYRKNKRTWGSCNYKNELNFNILLMKFPLYIMEYIVI
                     HELAHIKHKNHSKKFWELVEKYSPNYKEIEKVFKTLL"
     gene            complement(1392..>1701)
                     /locus_tag="PKO10_05110"
     CDS             complement(1392..>1701)
                     /locus_tag="PKO10_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HNW66558.1"
                     /translation="YIKNQTMHFVLTHQLFKVEFEHNKDTLKALLKMADIKDVSNFVF
                     FVSNSVDNLIKKNSENIDKKYIEKSVGIFENRAKDEKIFKKFESIREIIKNSTNQSSI
                     "
BASE COUNT          606 a          220 c          200 g          675 t
ORIGIN      
        1 agatttgctc aaaccattta ttgtaactgt tctattgtac atatcttcac taacttcagc
       61 agttgcagtg aattttttac ctttataaat aagtttttca tacatttcat ctgataaaac
      121 tacaatatct gtacctttta aaacttcacc aatagctaat aactcatctc ttgagtaaat
      181 tgaacctgtt ggatttgaag gagagtttaa cattaaaatt tttgtttttg gtgtaatttt
      241 ctcttttaat tgttttgcag taattttgaa ttctgtactc tcatctgttt ctataaatac
      301 aggaactcct ccactataaa caactaattc tggataagta acccaatatg gactaggaat
      361 tataacttca tcaccttcat caattagtgc ttgagctaga ttaaataaag agtgtttagc
      421 tccattactt attacaattt tatccaatgt ataatctaaa ttgtggtctt tctttaactt
      481 attaataatt gcttgtttag tttctttaat accctcaact gctgtatatt tagtttttcc
      541 ttcatttatt gctttaatag ctgcatcttt tacaacttgt ggcgtatcaa aatcaggttc
      601 acctgcacta aaacttagta tatctttacc agcagctttt aattcgtttg ctaaagctgt
      661 tatagataaa gtaagtgaag gagctaattt ctccattcta tttgcaattt tcataatagt
      721 tttcctcaat taaattatca ttacacttat ggtataatcg ggcggatttt atctaaaatt
      781 tacttaaagg ttttttttga attttgaaat taatttgaat aatagtgtaa taagtgttga
      841 attacataat aaaaagcata taaaacattg ctatttaaga attttaagaa aagatttatt
      901 gcaaataaaa gcaaatagat attttacaat ttatgatgca aaagatttga tagatagaaa
      961 aaaagattgg atattagaaa atataaaaag agtagaaagt aaaactttag aagatggtta
     1021 ttttttatat ttaggagaaa aaaagctttt aagcgatttt gctataaaaa atcttgatag
     1081 tttttataaa aaagagatag acagttttat ctctactttt attgaaaaat attcaaattt
     1141 aatgcaacta tttccaacaa aaatttcata tagaaaaaac aaaagaactt ggggttcttg
     1201 taactataaa aatgagctaa attttaatat attgcttatg aaatttccac tttatattat
     1261 ggaatatatt gttattcacg aactagcaca tataaaacat aaaaaccatt caaaaaagtt
     1321 ttgggaatta gtagaaaagt actctccaaa ctataaagag atagaaaaag tatttaaaac
     1381 tcttttataa tttaaatact actttgatta gttgaatttt ttataatctc tcttatactt
     1441 tcaaattttt taaatatttt ttcatctttt gccctatttt caaaaattcc aacacttttt
     1501 tctatatatt ttttatctat attttcactg ttttttttga ttaaattatc tacactattt
     1561 gatacaaaaa aaacaaaatt tgaaacatct tttatatctg ccatttttaa aagagctttt
     1621 agtgtatctt tgttatgctc aaactcaact ttgaaaagtt ggtgagttag aacaaaatgc
     1681 atggtttgat ttttaatata t
//
LOCUS       DAOQAN010000293         1697 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_109085_length_1697_cov_2.027406, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000293 DAOQAN010000000
VERSION     DAOQAN010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109085_length_1697_cov_2.027406"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="PKO10_05115"
     CDS             complement(<1..549)
                     /locus_tag="PKO10_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012148049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNW66559.1"
                     /translation="MIDKKEFLLQSIIKAYIEHLEPIGSKELKSMYELDYSPATIRGY
                     FKKLGDEGFLAQEHISSGRTPTNEALKQYWIGKLNFSISGVNIKAIEFLANKIGVTVF
                     LKKEKSDILQNIINVENRYIILEFTTFVVNIKFNPALYKNPETDAPIQYSIAKSALIH
                     LTKELAVRFADKNIQVNAISYGG"
     gene            complement(666..1316)
                     /locus_tag="PKO10_05120"
     CDS             complement(666..1316)
                     /locus_tag="PKO10_05120"
                     /inference="COORDINATES: protein motif:HMM:NF017815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like cysteine peptidase"
                     /protein_id="HNW66560.1"
                     /translation="MRKLIFLTILSSLFFTNSFAYQFALNSKDKNLINNSKQKSAILK
                     RLEKYSEFKNETKNLDINKKLGKVNFFINKTLPEFDTQSVGIDDYWMTPKEFFIKGFG
                     DCEDYAIAKYFTLLELGVKKESLYLAVVNVKASAGTHMVLLYVENKNSSPLVLDNLSF
                     RVLPFSKRDDLTPVVAFNELNAYEFTKDKFTQLVTIDWQNNNKWDRVLNRVYKLNE"
     gene            complement(1313..>1697)
                     /locus_tag="PKO10_05125"
     CDS             complement(1313..>1697)
                     /locus_tag="PKO10_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012148051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HNW66561.1"
                     /translation="SKPTTPFNAKFVTYIKNIDEELLKSAIKEFIGVYDFKYFHKTGS
                     EKDNTKREIFDTKFYKYKDIYVFKFCANSYLRTQIRLMVGFLIAINDKKLTIEDLKKQ
                     LNCEKNIFKTPIAPNGLYLARIFYK"
BASE COUNT          576 a          250 c          171 g          700 t
ORIGIN      
        1 ccctccgtaa gaaatagcgt ttacttgaat atttttatct gcaaatctga ctgcaagctc
       61 ttttgttaaa tgaattaaag cagattttgc aatactgtat tgtatcggag catctgtttc
      121 gggattttta tataaagccg gattaaactt tatatttaca acaaaagttg taaactctaa
      181 aattatatac ctgttttcta catttattat attttgtaaa atatcacttt tctctttttt
      241 taaaaaaact gttactccaa ttttattagc caaaaactca atagctttta tattcacacc
      301 agaaatagag aaatttaatt ttcctatcca atactgtttt aaagcttcat ttgtgggtgt
      361 tcttccacta cttatatgct cttgagctaa aaaaccttca tctcctagtt ttttaaaata
      421 tccacgaatt gttgcaggag aataatctaa ctcatacata gatttaagct cttttgaacc
      481 tattggttct aaatgttcta tatatgcttt aataatagat tgtaataaaa actctttttt
      541 atctatcata aaatcctgct ttttctaaac tttagcactc tatcatataa actgctaaag
      601 aattatatct tattgagtca atgtttgtca agtatttatt ctacaaatta taaatataga
      661 tattttcact catttaattt ataaactcta tttaatactc tatcccattt attgttattt
      721 tgccaatcaa tagttacaag ttgtgtaaat ttatcttttg taaattcata tgcatttaac
      781 tcattgaaag ctacaacagg agttaaatca tctctttttg aaaaaggaag aactctaaaa
      841 ctaagattat ctaaaactag tggggaagag tttttatttt caacatataa taaaaccata
      901 tgtgttccag cactagcttt cacatttaca acagctaaat acaaactctc tttttttact
      961 ccaagttcca aaagtgtaaa atattttgct attgcataat cttcacaatc tccaaaccct
     1021 ttaataaaga actcttttgg tgtcatccaa taatcatcta ttccaacact ttgagtatca
     1081 aattcaggca aagttttatt tataaaaaaa ttgactttcc ctaatttttt attaatatca
     1141 agattttttg tctcattttt aaattcacta tatttttcta atctttttaa aatagcactt
     1201 ttttgttttg agttatttat taaattttta tcttttgaat ttagtgcaaa ttgatatgca
     1261 aagctatttg taaaaaatag agagcttaat atagttaaaa aaatcaattt tctcatttat
     1321 aaaaaatcct agctagatat aaaccatttg gtgcaattgg tgttttaaat atattttttt
     1381 cacaatttaa ctgttttttt aaatcttcta ttgttagctt tttatcatta attgctatta
     1441 aaaatcctac cataagtcgt atttgagttc ttaaataaga gtttgcacaa aatttaaaaa
     1501 cataaatatc tttatattta tagaattttg tatcaaaaat ctctcttttg gtattatctt
     1561 tttcacttcc tgttttatga aaatatttaa aatcataaac tccaataaac tcttttatgg
     1621 cactttttaa aagttcttca tctatatttt ttatataagt tacaaattta gcattaaaag
     1681 gagttgttgg ttttgaa
//
LOCUS       DAOQAN010000294         1696 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_109167_length_1696_cov_2.411335, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000294 DAOQAN010000000
VERSION     DAOQAN010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109167_length_1696_cov_2.411335"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="PKO10_05130"
     CDS             <1..594
                     /locus_tag="PKO10_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014473349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNW66562.1"
                     /translation="KKNLKDKKYSFLFDKPLENEYVCFDCETTGLDPQIDDIISIGAV
                     IIKNNTIVSSKKFVRYIKPKNCSLDEKSIKIHHIRECDLENGCEIEDFILEFLEFIGN
                     RTLVGYFLDFDKNMINKYLKPLLGITLPNRSIEVSEIYHDFKIELIPQSFIDLKFKTI
                     VNDLDIPEFGNHDSYNDAIMTAMIFLKLKNHPNVKIK"
     gene            595..1536
                     /locus_tag="PKO10_05135"
     CDS             595..1536
                     /locus_tag="PKO10_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HNW66563.1"
                     /translation="MNLSKFKSEKEILKLSIFTTIFLAILGLIFGLLASSATIIFDSI
                     YGMIDALMTVLALVVAKLITSSTSKDIVSNRLEKHFTMGFWHLEPIVLGVNGILLIGA
                     SIYAFINAIDSILLGGREIIFSYAIIITAISIVVEITLGVFIRKANKDINSEFLKLDS
                     ISWLISASMSFAYLIAFGFGYFVKDGELSWITPFIDPFILIFVSILVIPMPFKTVKQA
                     LADILLVTPSSLKNHVDVVAKNIVNKYKFDSFRAYVARVGRGRQIELYFIVPKSWPAK
                     RLEEWDMLRDEIEKDLGKEDPNLWLTIVFTTDFEWAE"
BASE COUNT          633 a          182 c          260 g          621 t
ORIGIN      
        1 aaaaaaaatt taaaagataa aaaatatagt ttcttatttg ataaaccttt agaaaatgag
       61 tatgtttgtt ttgattgtga aacaacagga ttagatccac aaattgatga tattatctct
      121 attggtgctg tgattataaa aaataataca atagtttcaa gtaaaaagtt tgtacgatat
      181 attaaaccta aaaactgctc tttagatgaa aaatcaataa aaatacacca tatacgagag
      241 tgtgatttag aaaatggatg tgaaatagag gattttattt tagagttttt agagtttata
      301 ggaaatagaa ctttagttgg atatttttta gattttgaca aaaatatgat aaataaatat
      361 ctaaaaccac tattgggaat aactctacca aatagaagta tagaggtgtc tgagatttat
      421 cacgatttta aaattgagtt aattccgcaa agctttatag atttaaaatt taaaactata
      481 gtaaatgatt tagatattcc agaatttgga aatcacgact cttataatga tgcaataatg
      541 actgcaatga tatttttaaa actaaaaaat catccaaatg taaaaataaa atagatgaat
      601 ttatcaaaat ttaaatcaga aaaagagata ctaaaactct ctatatttac cacaattttt
      661 ttagcaattc ttggtttgat ttttggtctg ctagctagtt ctgcaactat tatttttgat
      721 agtatttatg gaatgataga tgctttaatg actgttttag cacttgttgt tgcaaaatta
      781 attacatctt caacttcaaa agatattgtc tcaaatagac ttgaaaaaca ttttacaatg
      841 ggcttttggc accttgaacc aattgtttta ggagtaaatg gaatactttt aataggtgct
      901 tcaatttatg cttttataaa tgcaattgat agtattttac ttggaggaag agagataatc
      961 ttttcatatg caattattat aactgctatt tctattgtag ttgagattac tttgggagtt
     1021 tttataagaa aagcaaataa agatattaat tctgaatttt taaaattaga ttctattagt
     1081 tggttgattt cagcttccat gtcttttgct tatcttatag cttttggttt tggatacttt
     1141 gtaaaagatg gagagctttc ttggattaca ccatttattg acccatttat actaattttt
     1201 gtatcaattt tagtaattcc aatgcctttt aaaactgtta aacaagcact tgcagatatt
     1261 ttgcttgtaa ctccttcttc tttaaaaaat catgttgacg ttgttgcaaa aaatattgta
     1321 aataaatata aatttgactc ttttagagcc tatgttgcaa gagttggaag aggaagacag
     1381 attgaacttt attttatagt tccaaaaagc tggccagcaa aaagacttga agagtgggat
     1441 atgcttcgtg atgagataga gaaagattta ggaaaagagg atccaaatct ttggcttaca
     1501 atagttttca caactgattt tgagtgggca gaataaaaaa attatttaag ttttaattag
     1561 atattatttc aaaaagttaa ttataaattt taatataaat ttaagaaaaa ggatagaaat
     1621 gttgattttc gttttaaatg caggttcttc ttcactaaaa tatcaactaa tagatataaa
     1681 atcaaaagag ctaaaa
//
LOCUS       DAOQAN010000295         1694 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_109330_length_1694_cov_3.998170, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000295 DAOQAN010000000
VERSION     DAOQAN010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109330_length_1694_cov_3.998170"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..930
                     /gene="rpoD"
                     /locus_tag="PKO10_05140"
     CDS             <1..930
                     /gene="rpoD"
                     /locus_tag="PKO10_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HNW66564.1"
                     /translation="HQKILDNILNLSKAQITAMVPEATMVSTYMEIKKLFQTAEASKD
                     GFDLTPEELKAVLEQIKRGKKITDTSKDRMAKSNLRLVVSIAKRYTNRGLAFLDLIQE
                     GNIGLMKAVDKFEYKKGYKFSTYATWWIRQAISRAIADQARTIRIPIHMIETINRINK
                     IIRKGIQENGKEPDVEEIAKEVGLPVDKVKQVIKITKEPVSLEAPIGSDDDGKFGDFV
                     PDEKAPTPIDNIMKEDLQGQIDQILGQLNEREQAVIRMRFGLMDDASDRTLEEIGKEL
                     SVTRERVRQIESSAIKKLKHPKVGKNLKNYVES"
     gene            complement(971..>1694)
                     /locus_tag="PKO10_05145"
     CDS             complement(971..>1694)
                     /locus_tag="PKO10_05145"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HNW66565.1"
                     /translation="ENSELPSSIFSSIVAMSNKIDNLMALFSAGKIPTGSKDPFALRR
                     AALGVVKIAIEHKLDIDFKAIFEDLKENYKNLDLKVLNEFFIERLYKIFDSVNPTVLK
                     AVISSGERNIYKIYQKVEALNPFVESDNFKDYSATFKRVANIVKDIEINSSLNIEETL
                     FEQDEERDLYNSFKKIKTSNFVTFDEELEALFSLKPQLDSFFEKVFVNHEDEKIKTNR
                     KNLIAQVYLGFRNIADIKEITI"
BASE COUNT          662 a          226 c          237 g          569 t
ORIGIN      
        1 caccaaaaga ttctagataa tatattaaat ttatcaaaag ctcaaataac tgctatggtt
       61 cctgaagcta caatggtttc aacatatatg gagataaaaa aacttttcca aacagcagaa
      121 gctagtaaag atggttttga tttaacacct gaagagctaa aagctgtttt agagcagatt
      181 aaaagaggta aaaaaatcac tgatacttca aaagatagaa tggcaaaatc aaaccttaga
      241 cttgttgtat ctattgcaaa aagatataca aatagaggat tagctttctt ggatttaatt
      301 caagaaggaa acattggtct tatgaaagca gttgataagt ttgaatataa aaaaggttat
      361 aaattctcta cttatgctac atggtggatt agacaagcaa tctctagagc aattgcagat
      421 caagcaagaa ctattagaat tcctattcat atgattgaaa ctattaatag aatcaataaa
      481 attattagaa aaggtattca agaaaatggt aaagagccag atgttgaaga gattgcaaaa
      541 gaggttggat tacctgttga taaagtaaaa caagttataa aaatcacaaa agagcctgta
      601 tctttagaag ctccaatagg aagtgatgat gatggtaaat ttggagattt tgtaccagat
      661 gaaaaagctc caacaccaat tgataacatt atgaaagagg atttacaagg tcaaattgac
      721 caaattcttg gacaattaaa cgaaagagaa caagcagtta taagaatgag atttggtctt
      781 atggatgatg ctagtgatag aactcttgaa gagattggaa aagaactttc tgttacaaga
      841 gagagagtta gacagattga atcaagtgct attaaaaaat taaaacaccc aaaagttggt
      901 aaaaatctta aaaattatgt agagagttaa tctctacata aaataaaact atctaaaaaa
      961 tataaaaaaa ctaaattgtt atctctttaa tatctgcaat attcctaaat cctaaataaa
     1021 cttgtgcaat aagatttttt ctatttgttt taattttttc atcttcatga tttacaaaaa
     1081 ctttttcaaa aaagctatca agttgtggtt ttaaagaaaa tagagcttca agttcctcat
     1141 caaaagttac aaagtttgaa gtttttattt ttttgaaact attatataaa tctctctctt
     1201 catcttgttc aaatagtgtt tcttcaatat ttaaagaact attaatctca atatctttta
     1261 caatatttgc aactctttta aatgtagcac tataatcttt aaaattatca ctttcaacaa
     1321 atggatttaa agcttctact ttttgataaa ttttataaat atttctctct ccactactta
     1381 ttacagcttt taaaacagtt ggatttacac tatcaaaaat tttataaagt ctttctataa
     1441 aaaattcatt taaaactttt aaatcaagat ttttataatt ttcttttaaa tcttcaaaaa
     1501 tagctttaaa atctatatcc aatttatgtt ctattgcaat ttttacaact cctaaagctg
     1561 ctcttcttaa tgcaaatgga tcttttgaac ctgttgggat ttttccagca ctgaaaagtg
     1621 ccataagatt atctatttta tttgacatag caactattga agagaaaatt gaactaggaa
     1681 gttcactatt ctca
//
LOCUS       DAOQAN010000296         1694 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_109378_length_1694_cov_2.601586, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000296 DAOQAN010000000
VERSION     DAOQAN010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109378_length_1694_cov_2.601586"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="PKO10_05150"
     CDS             <1..499
                     /locus_tag="PKO10_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HNW66566.1"
                     /translation="RDLLNNALENKDIDIAKYVRPIIMVPENTSISTILTRMNKSRIH
                     LALVVDEYGGTSGLITLDDIIEEIIGETTDEHDPKNETIKKIDENKYELDGMVNIEKV
                     EEILDIRFEETEISVTIGGRIFNLIGRLPVAGETIEDKYCTYKILEVGSNRIKRILCE
                     KIVEE"
     gene            complement(515..>1694)
                     /locus_tag="PKO10_05155"
     CDS             complement(515..>1694)
                     /locus_tag="PKO10_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HNW66567.1"
                     /translation="SNFKSINAVLDDPQSVKNDISTFAGRMLEFSKLFEVKNAIVGVD
                     EIELGTDSDEAASLFKVIIEDLIKNDIKIIITTHHKRLAALMASNPNVELIAALYDEE
                     NQKPTYQFLQGTIGRSYAFETALRYKIPAGVVKRAKEVYGEDKDRLNELIERSSELER
                     EYRQKIANLDSEIENYKRLSNNLKEQKESLDLHIYSEKSKLHKEYQDARDEAKKAIKA
                     KLVSQSHQHLNISHKIAKEIEVEKVQEKIELKVGDRVKYRNTKGTIVSIKGSKAFIEN
                     DMGMKVQVQLNDLSRSGNPPPKIPTKKAVVTIQKPETGNIKLDLHGLRAEEAIENLDK
                     FLSDALLAGFEEVLVYHGIGSGKLAFAVKEFLKKHPRVKGFEDAHPSSGGFGAKVIKL
                     "
BASE COUNT          560 a          267 c          215 g          652 t
ORIGIN      
        1 tagagattta ttaaataacg ctcttgaaaa taaagatata gatattgcaa aatatgtaag
       61 accaattatt atggtgcctg aaaatacatc aatctctact attttaacaa gaatgaataa
      121 aagtagaatt catttagcat tagttgttga tgagtatggt ggaacttctg gtcttattac
      181 tttagatgat attattgaag agattattgg tgaaacgaca gatgaacatg atccaaaaaa
      241 tgaaactatt aaaaaaatag atgaaaataa atatgaacta gatggtatgg taaatattga
      301 aaaagttgaa gagattttag atataagatt tgaagagact gaaatatctg ttactattgg
      361 tggaagaata tttaatttaa taggtagatt accagtagct ggagaaacta ttgaagataa
      421 gtattgtact tataaaattt tagaagtagg aagtaatcgt ataaaaagaa tattatgcga
      481 aaaaatagta gaagaataat atcttaaaat agagctaaag ttttataact ttagccccaa
      541 atccaccact acttggatgt gcatcttcaa aaccttttac tcttggatgt tttttcaaaa
      601 actcttttac agcaaaagct aattttccag aaccaatccc gtgatatact aaaacttctt
      661 caaatccagc caaaagagca tcacttaaaa acttatcaag attctcaata gcctcttctg
      721 ctctaagacc atgtaaatct aactttatat ttcctgtttc tggcttttga atagttacaa
      781 cagctttttt tgtaggaatt tttggtggag gatttccact tctacttaaa tcattaagtt
      841 gaacttgaac tttcattccc atatcatttt caataaatgc cttagaacct ttaattgaaa
      901 caatagttcc ttttgtattt ctatatttaa ctctatctcc aacttttagc tcaatttttt
      961 cttgtacttt ttcaacttct atctcttttg ctattttgtg tgaaatattt aaatgttgat
     1021 gtgattggct aacaagtttt gcttttatag ccttttttgc ttcatctcgt gcatcttgat
     1081 actctttatg aagctttgat ttttcactat aaatatgtaa atctaaactc tctttttgct
     1141 cttttaaatt atttgataat cttttataat tttctatttc actatctaaa tttgctattt
     1201 tttgcctata ctcacgctca agctcactac ttctttctat caactcattt aatctatctt
     1261 tatcttctcc ataaacctct tttgcccttt ttacaactcc cgctggaatt ttatatctca
     1321 atgctgtttc aaaagcatag gatcttccaa tagttccttg taaaaactga tatgttggtt
     1381 tttgattctc ttcatcataa agtgctgcta ttaactcaac atttgggttt gaagccatca
     1441 aagcagccaa tcttttatga tgagttgtaa ttattatttt tatatcattt ttaattaaat
     1501 cttcaattat tactttgaat aagcttgctg cttcatctga atcagtacct aattctatct
     1561 catcaactcc aactattgca ttttttactt caaaaagctt tgaaaattct aacattctac
     1621 cagcaaatgt ggaaatatca tttttaacac tttgaggatc atctaaaaca gcatttatag
     1681 atttaaaatt actt
//
LOCUS       DAOQAN010000297         1692 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_109566_length_1692_cov_3.286500, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000297 DAOQAN010000000
VERSION     DAOQAN010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109566_length_1692_cov_3.286500"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            21..389
                     /gene="rpsM"
                     /locus_tag="PKO10_05160"
     CDS             21..389
                     /gene="rpsM"
                     /locus_tag="PKO10_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009494839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HNW66568.1"
                     /translation="MARIAGVDLPNKKRMEYALTYIFGIGLHNSRLILNATGIDYNKR
                     AFELTEDEAALIRKEIQENYLVEGDLRKKVAMDIKSLMDLGSYRGLRHRKGLPCRGQK
                     TKTNARTRKGKKKTIGAASK"
     gene            399..791
                     /gene="rpsK"
                     /locus_tag="PKO10_05165"
     CDS             399..791
                     /gene="rpsK"
                     /locus_tag="PKO10_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002344962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HNW66569.1"
                     /translation="MAKRKVTRKKIVRKNIADGIVHIAASFNNTMVTVTDNMGNAIAW
                     SSAGNLGFKGSKKSTPFAAQAAVEDAMAKAMEHGIKNVGIKIQGPGSGRDTAVKSVGA
                     ISGIRVTWLKDVTPLAHNGCRPPKRRRV"
     gene            802..1428
                     /gene="rpsD"
                     /locus_tag="PKO10_05170"
     CDS             802..1428
                     /gene="rpsD"
                     /locus_tag="PKO10_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002856574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HNW66570.1"
                     /translation="MARYRGPVEKIERRLEADLGLKGERRLSGKSALEKRPFAPGQHG
                     QRRTKISEYGLQLREKQKVKFMYGISEKQFSNYFKEAVRREGNTGSILITLIEQRLDN
                     VVYRMGFATTRAFARQITTHGHILVDGKKVDIPSYIVKPGQKIEIREKSKNNPQIKRA
                     IELTNQTGMVDWVNVDKDKVFGIFTRIPAREEIVIPVEERLIVELYSK"
     gene            1447..1692
                     /locus_tag="PKO10_05175"
                     /pseudo
     CDS             1447..1692
                     /locus_tag="PKO10_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014474479.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
BASE COUNT          685 a          207 c          345 g          455 t
ORIGIN      
        1 caaagacaag gataagacac atggcaagaa tcgcgggtgt tgatttacca aacaaaaaaa
       61 gaatggagta tgctttaaca tacatctttg gaattggatt acataattca agactaatct
      121 taaatgcaac tggaattgat tacaataaaa gagcattcga attaacagaa gatgaagctg
      181 ctttaattag aaaagagatt caagaaaact acctagtaga aggggatctt agaaaaaaag
      241 ttgctatgga tattaagtct ttaatggatt taggttcata tagaggatta agacacagaa
      301 aaggtttacc ttgtagaggg caaaagacta aaactaatgc aagaacaaga aaaggtaaaa
      361 agaaaactat tggtgcagca agtaaataag gatagataat ggcaaaaaga aaagttacta
      421 gaaaaaaaat tgtaagaaaa aatattgctg acggtatcgt tcatatagct gcaagtttta
      481 acaatacaat ggttacagtt acagataaca tgggaaatgc aatagcatgg tcaagtgctg
      541 gaaacttagg gttcaagggt tctaaaaaat caactccatt tgctgctcaa gctgcagttg
      601 aagatgctat ggcaaaagca atggaacatg gaattaaaaa tgtaggaatt aaaattcaag
      661 gtcctggttc tggaagagat actgctgtta aatcagttgg agctatcagt ggaattagag
      721 ttacttggtt aaaagatgtt acaccattag cacataatgg ttgtagacct cctaaaagaa
      781 gaagagtgta aggagtaaaa aatggcaaga tatagaggac cagtagaaaa aatcgaaaga
      841 agacttgaag cagaccttgg tttaaaaggt gagagaagac tttcaggtaa aagtgcttta
      901 gaaaaaagac catttgctcc tggacaacat ggacaaagaa gaactaagat ttctgagtat
      961 ggattacaat taagagaaaa acaaaaagtt aaatttatgt atggtatttc tgaaaaacaa
     1021 ttcagtaact atttcaaaga agcagttaga agagaaggaa atacaggatc aattttaatt
     1081 actctaattg agcaaagatt agataatgtt gtttacagaa tgggatttgc tacaactaga
     1141 gcatttgcta gacaaataac aactcacgga catatcttag tagatggtaa aaaagttgat
     1201 attccatcat acatagtaaa acctggacaa aaaattgaga ttagagaaaa atctaaaaat
     1261 aatccacaaa ttaaaagagc tatcgagctt acaaatcaaa caggaatggt tgattgggta
     1321 aatgtggata aagataaagt atttggaatt ttcacaagaa tacctgctag agaagagata
     1381 gttattccag ttgaagagag attaattgtt gagttatact ctaaataaaa aataaaaagg
     1441 tagccaatga aaaagtttgc agaaactccg tttttaccaa ctgaagttga aattgaggct
     1501 attagtgcta atgaagctaa aattacagct tatccatttg aagatggatt tgctataact
     1561 ttagctcatc cgttaagaag attactttta agttcatctg ttggttatgc accaattgct
     1621 gttaaaattg aaagcgttac tcatgagttg actcattaag aggaatgctt gaagatgttg
     1681 ctatgtttgt ta
//
LOCUS       DAOQAN010000298         1691 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_109628_length_1691_cov_14.543399, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000298 DAOQAN010000000
VERSION     DAOQAN010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109628_length_1691_cov_14.543399"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="PKO10_05180"
     CDS             <1..506
                     /locus_tag="PKO10_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66571.1"
                     /translation="SKYQKLSEEFIKEFSDKVNWDSISAYQKLSESFIKEYKLTIPKT
                     NWQYKIKEEKLSYLKENCNGIYEIVDDNYIVAYKSTRVDGYSTFNFQYQYKENEVYES
                     HCDCNINDENSFGLSAWTKEKALEHYSKGKLFKVHINIEDIGAIVHDNKKIRCSKLTV
                     ISEESFE"
     gene            518..1492
                     /locus_tag="PKO10_05185"
     CDS             518..1492
                     /locus_tag="PKO10_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66572.1"
                     /translation="MNNQIFEEMKSIAKDYDIVFLDESDIEKCKQEFVDKVDWEYISI
                     YQKLSEGFIREFADKVYWEHISIRQKLSEGLIREFADKVNWCYISMYQKLSEGLIREF
                     ADKVDWGYISRYQPLSEGLIREFADKVDWGYISRYQPLSEGFIREFADNVYWDYISYS
                     QKLSEEFIREFADKVNWYCISEYQKLSEEFVKEHQLTILEDNWLYKTKEDKLSYLKEN
                     CNGIYEIVDDNYIVAYKSTRVDGYSTFNFQYQYKENEVYESHCDCNINDENSFGLSAW
                     TKEKALEHYSKGKLFKVHINIEDIGAIVHDNKKIRCSKLTVISEESFE"
     gene            1504..>1691
                     /locus_tag="PKO10_05190"
     CDS             1504..>1691
                     /locus_tag="PKO10_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66573.1"
                     /translation="MNNQIFEEMKSIAKDYDIVFLDESDIEKCKQEFVDKVDWEYILE
                     HQKLSEGFIREFADKVNW"
BASE COUNT          703 a          147 c          285 g          556 t
ORIGIN      
        1 tttcaaaata tcaaaaacta tcagaagaat ttattaaaga attttctgat aaagtgaatt
       61 gggatagtat ttcagcttac caaaaactat cagaatcttt tattaaagaa tacaaattaa
      121 caataccaaa aactaattgg caatataaaa taaaggaaga aaagctatct tatttgaaag
      181 aaaattgtaa tggtatttat gaaattgtag atgataatta tatagtagca tataaatcaa
      241 caagagttga tggatattct acattcaatt ttcaatatca atataaagaa aacgaggtat
      301 atgaatctca ttgtgattgt aatataaatg atgagaattc atttggattg tcagcttgga
      361 caaaagaaaa agcattggaa cattatagta aaggtaaact attcaaagtc catattaata
      421 tagaagatat tggagcaatt gttcatgata ataagaaaat tcgttgcagt aaactaactg
      481 ttatttctga agaatctttt gaataggaag aaatataatg aacaatcaaa ttttcgaaga
      541 aatgaaatca attgcaaaag attatgatat tgtttttttg gatgaaagtg atattgaaaa
      601 atgtaaacaa gaatttgttg ataaagtaga ttgggaatat atttcaatat atcaaaaatt
      661 gtcagaagga tttattcgag aatttgctga taaagtatat tgggaacata tttcaattcg
      721 tcaaaaacta tcagaaggat tgattcgaga atttgctgat aaagtaaatt ggtgttatat
      781 ttcaatgtat caaaaactat cagaaggatt gattcgagaa tttgctgata aagtagattg
      841 gggttatatt tcaagatatc aaccattatc agaaggattg attcgagaat ttgctgataa
      901 agtagattgg ggttatattt caagatatca accattatca gaaggattta ttcgagaatt
      961 tgctgataat gtatattggg attatatttc atattctcaa aaactatcag aagaatttat
     1021 tagagaattt gctgataaag taaattggta ttgtatttca gaatatcaaa aactatcaga
     1081 agaatttgtt aaagaacatc aattaacaat tctagaagat aattggttgt ataaaacaaa
     1141 agaagataag ctatcttatt tgaaagaaaa ttgtaatggt atttatgaga tagtagatga
     1201 taattatata gtagcatata aatcaacaag agttgatgga tattctacat tcaattttca
     1261 atatcaatat aaagaaaacg aggtatatga atctcattgt gattgtaata taaatgatga
     1321 gaattcattt ggattgtcag cttggacaaa agaaaaagca ttggaacatt atagtaaagg
     1381 taaactattc aaagtccata ttaatataga agatattgga gcaattgttc atgataataa
     1441 gaaaattcgt tgcagtaaac taactgttat ttctgaagaa tcttttgaat aggaagaaat
     1501 ataatgaaca atcaaatttt cgaagaaatg aaatcaattg caaaagatta tgatattgtt
     1561 tttttggatg aaagtgatat tgaaaaatgt aaacaagaat ttgttgataa agtagattgg
     1621 gaatatattt tagaacatca aaaactatca gaaggattta ttcgagaatt tgctgataaa
     1681 gtaaattggc a
//
LOCUS       DAOQAN010000299         1691 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_109660_length_1691_cov_3.254890, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000299 DAOQAN010000000
VERSION     DAOQAN010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109660_length_1691_cov_3.254890"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /gene="flgB"
                     /locus_tag="PKO10_05195"
     CDS             <1..342
                     /gene="flgB"
                     /locus_tag="PKO10_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HNW66574.1"
                     /translation="ERQKVISSNIANVNTPEYKTKDLVFSEELKEQNILKLKRTTNNH
                     MHNLDYRASSNPRLIEVPNLIEQNDGNNVNLDSQMSEQSKNKVLFDAIQSSIKKDSRL
                     FRSVIDASGKS"
     gene            358..>1691
                     /gene="fliF"
                     /locus_tag="PKO10_05200"
     CDS             358..>1691
                     /gene="fliF"
                     /locus_tag="PKO10_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013136296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HNW66575.1"
                     /translation="MDQLLKFINNLNKAQRIAIVGGFAFLLLLIIGFLIYSNIKAEDK
                     KLSYTIASNLTQADVMRATEELESAGVPFIVTGTGSNLTLKTSKEFINIAKIKLVTSE
                     ASTNKHVGWEIFEKSSIGTTNFENKVKYLRALEGELSRSLESLSGVLKADVKIAIPKE
                     TIFTEKKSDTTASAVLTLKQGIFLTQKQLDGIKNFIASAVPDLKQENIQLIDQDGNLL
                     EVSAEDMNTQRSTVQTKFKDKIEEDYEQKIISLLEPVVGAGRVMAKVNVILDFTKKDI
                     EEEIYSPEGSIRSQQVIENTSNASGLGNNTGGIAGADNNIQPPQQLNDGSKLSSNSES
                     SNTVTNYEISRKLISQKDGNFTNIRRISASVTFDSGVLKDHPDKAEFLASLESLSADA
                     IGYDKGRGDTITVKDFKFVGVKSFNEKGELVDEFGNVVGGSGYLQGMSIKSIL"
BASE COUNT          675 a          190 c          298 g          528 t
ORIGIN      
        1 gaaagacaaa aagtaatatc tagcaatatt gcaaatgtta atactccaga atacaaaaca
       61 aaagatttag ttttttcaga agagttaaaa gagcaaaata ttttaaaatt aaaaagaact
      121 acaaataacc atatgcacaa tcttgattat agggctagca gtaacccaag attaattgag
      181 gttccaaatt taatagaaca aaatgatgga aacaatgtaa atttagatag tcaaatgagt
      241 gagcaatcaa aaaataaagt tctttttgat gcaatacaat catctataaa aaaagattca
      301 agacttttta gatctgttat tgatgcttca ggtaagagtt aataggatga ttttttaatg
      361 gatcaacttc taaaatttat aaataactta aataaagcac aaagaatagc aattgttggt
      421 ggttttgctt ttttgctgtt attaattatt ggatttttga tatattcaaa tatcaaagct
      481 gaagataaaa aattaagtta cacaattgct tcaaatctta ctcaagctga tgttatgaga
      541 gctactgagg agcttgaaag tgctggtgtt ccatttatag taacaggaac tggaagtaat
      601 ttaactctta aaacttcaaa agagtttata aatattgcaa aaattaaact tgtaactagt
      661 gaagcttcaa caaataaaca tgttggatgg gaaatttttg aaaaatcatc tataggtaca
      721 acaaattttg aaaataaagt aaagtatcta agagctttag agggagaact aagtcgtagt
      781 ttagagtctt taagtggtgt tttaaaagca gatgtaaaaa tagcaattcc aaaagagaca
      841 atatttactg agaaaaagag tgatacaact gcctctgctg ttttgacact aaaacagggt
      901 atttttttga ctcaaaaaca actagatggt attaaaaact ttatagcttc tgctgttcct
      961 gatttaaagc aagaaaatat acagttaata gatcaagatg gaaatttact tgaagtatca
     1021 gctgaagata tgaatactca aagatctaca gttcaaacga aatttaaaga taaaatagaa
     1081 gaggattatg agcaaaaaat tatttcactt ttagagccag ttgttggagc tggaagggtt
     1141 atggcaaaag taaatgttat tttggatttt actaaaaaag atattgaaga ggaaatttat
     1201 agtccagaag gaagtattag atctcaacaa gttattgaaa atacatcaaa tgcttcaggg
     1261 cttggaaata atacaggtgg aattgcagga gctgataata atattcaacc accacaacag
     1321 ctaaatgatg gaagtaaact atcttcaaat agtgaaagtt ctaatactgt tacaaactat
     1381 gaaatttcaa gaaaactaat atctcaaaaa gatgggaatt ttacaaatat tcgaagaatt
     1441 tcagcttctg ttacttttga ttcaggtgtt ttaaaagatc atccagataa agctgaattt
     1501 ttagcatcct tagaatcatt atctgctgat gctattggat atgataaagg aagaggcgat
     1561 acaattactg ttaaagattt taaatttgta ggagttaaat cttttaatga aaaaggtgag
     1621 cttgtagatg agtttggaaa tgtagttggt ggaagtggtt atttacaagg aatgagtata
     1681 aaaagtattc t
//
LOCUS       DAOQAN010000300         1687 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_110098_length_1687_cov_2.286765, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000300 DAOQAN010000000
VERSION     DAOQAN010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110098_length_1687_cov_2.286765"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(13..1602)
                     /gene="argS"
                     /locus_tag="PKO10_05205"
     CDS             complement(13..1602)
                     /gene="argS"
                     /locus_tag="PKO10_05205"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNW66576.1"
                     /translation="MQNIVKEFIEKKLEKSIVLEKPKDSSLGHFATPVAFSLAKELKK
                     SPMILADELVLKLENSELFEKIEAVKGFINFTLSKSFIEKLTNEALEKKDDFAKGDKK
                     DEKILLEYVSANPTGPLHIGHARGAVFGDTLYKVGIYLGYDITTEYYINDAGAQMQLL
                     GISVSLAARDFIFKEKVKYPESYYRGEYLIEIAEKIIEKHGKDIIYDESRFEEMAIFA
                     KDIVMQIIIKDLGDLGINFQNFVSEKSLYSSWENTKSVLEKNGSLYTKDEKVYLSSTK
                     FGDDSDRVVVRENGIPTYLAGDIIYHKNKFDREFDKYINIWGADHHGYITRVKAAIEF
                     LGNDSSKLEVILSQMVQLLKGGQPYKMSKRAGNVILMSDITSEIGSDALRFIFLTKKS
                     DTHLEFDIDMLKNQDSSNPIFYINYAHARINQVFVKSNLSFEDIKNESFEALNSEALN
                     LVYESLLLKSVLEDSFSKRDMQKITEYLYNLASSVHKFYNEHKIIGSSEEKIYLKVLS
                     MAKLSIKTGLKLLGIEAKEIM"
BASE COUNT          536 a          283 c          169 g          699 t
ORIGIN      
        1 aaacaaaaga gattacataa tctcttttgc ttcaattcca agaagtttaa gccctgtttt
       61 tatacttaat tttgccatac ttaaaacttt taagtaaatc ttttcttcac tacttccaat
      121 aattttatgt tcattataaa atttatgtac gctagaagct agattgtata aatattctgt
      181 tattttttgc atatctcttt tagaaaatga atcttctaaa acagatttta aaagaagtga
      241 ttcataaact aaatttaaag cttcactatt taaagcttca aagctctcat tttttatatc
      301 ttcaaaactt aaatttgatt ttacaaaaac ttgatttatt cttgcatgtg catagtttat
      361 ataaaaaatt ggattacttg agtcttgatt ttttaacata tcaatatcaa actccaagtg
      421 agtatcactt tttttagtta agaatataaa tcttaatgca tcacttccaa tctcagaagt
      481 tatatcagac attaatataa cattcccagc tcgtttactc attttataag gttgtccacc
      541 tttaagtagc tgaaccattt gagataaaat aacttcaagt ttagaagagt catttcctaa
      601 aaactcaata gctgctttta ctcttgtgat atatccgtgg tgatcagcac cccaaatatt
      661 tatatatttg tcaaactctc tatcaaattt atttttatga tagattatat ctcctgctag
      721 atatgttgga attccatttt ctcttacaac aactctatcg ctatcatcac caaatttagt
      781 tgagcttaga taaacttttt catcttttgt gtaaagtgaa ccattttttt ctaaaacaga
      841 ttttgtattc tcccatgagc tgtataaaga tttttcagat acaaaatttt ggaaatttat
      901 tcctaaatca cctaaatctt ttatgattat ttgcattact atatcttttg caaaaattgc
      961 catctcttca aatctactct catcatagat aatatctttt ccatgttttt ctatgatttt
     1021 ttcggcaatc tctattaaat attcgcctct gtaataactc tctggatatt ttactttctc
     1081 tttaaatata aaatctctag cagctaaact aacgcttatt cctaaaagtt gcatctgtgc
     1141 accagcatca tttatataat actcagttgt aatatcatat cctagataaa ttccaacttt
     1201 atataaagta tctccaaaaa cagcacctct tgcatgtcca atatgaagag gacctgttgg
     1261 atttgcactt acatactcta gaagtatttt ttcatctttt ttatcacctt ttgcaaaatc
     1321 atcttttttt tctaaggctt catttgtaag tttttctata aaactttttg ataaagtaaa
     1381 gtttataaaa ccttttacag cttctatttt ctcaaataat tcactatttt caagttttaa
     1441 aactaattca tctgctaaaa tcataggaga cttttttagc tcttttgcca aagaaaaagc
     1501 aactggtgtt gcaaaatgac caagtgaact atcttttggc ttttctaaaa caatactttt
     1561 ttctaatttt ttctctataa attctttaac tatattttgc aagttcttct cttattattt
     1621 attataaaat tcatatatta tgaatttttt gtctcttctt tttgtgtttg tatttcagct
     1681 tttttat
//
LOCUS       DAOQAN010000301         1687 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_110115_length_1687_cov_1.835784, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000301 DAOQAN010000000
VERSION     DAOQAN010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110115_length_1687_cov_1.835784"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="PKO10_05210"
     CDS             <1..201
                     /locus_tag="PKO10_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HNW66577.1"
                     /translation="FDFRERMEDIYDKSFRMIENIENSNIFKIIIEHESKLEVPKILG
                     KFSLEKTRKFGKSSLSYFSYKA"
     gene            201..1001
                     /locus_tag="PKO10_05215"
     CDS             201..1001
                     /locus_tag="PKO10_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014473842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNW66578.1"
                     /translation="MFRFSLILLIVVALGMFIAPIFYTVSPYELNPNKILLSPSLEHI
                     MGTDRLGRDVFARILQGGQTSLIIGFLAASFSSFLGLIIGITAGYFKGNIDKTITIVI
                     DLFLTFPTFFLLLALVSYIEANSLVLILVISITGWMGMSRMIRSESFALSNKPFIKIL
                     KIANVSKTKIILKYFAPLLAPIFLISFSFGVAGAILAESGLSFLGLGVNPPQMSWGSL
                     LSDGKAVIDIAWHLSFFPGLMIFIITFCLIQISDYLQNIANKKDLLKN"
     gene            1087..>1687
                     /locus_tag="PKO10_05220"
     CDS             1087..>1687
                     /locus_tag="PKO10_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HNW66579.1"
                     /translation="MSKKDIKKVVLAYSGGLDTSIILKWLQDEYDAEVITFTADLGQG
                     EEVEPARAKAIACGIKPENVYILDVKEEFVKDYVFPMFRANAIYEGEYLLGTSIARPL
                     IAKKLVEIANEKGAQAVSHGATGKGNDQVRFELGALALNPDLKVIAPWREWELNSRES
                     LLEYAKKHGIEISQKHVDENGNPKISPYSMDANLLHISYE"
BASE COUNT          611 a          207 c          285 g          584 t
ORIGIN      
        1 tttgatttta gagaaagaat ggaagatatt tatgataaat cttttagaat gattgaaaat
       61 attgaaaata gtaatatttt caaaataata atagaacatg agtcaaaact tgaagttcca
      121 aaaattttag gaaaatttag tttagaaaaa actagaaagt ttggtaaaag ttcactatct
      181 tactttagtt ataaagctta atgtttagat tttctttaat tttattaata gttgtagctt
      241 taggtatgtt tatagctcca attttttata cggtttctcc atatgaatta aatccaaata
      301 aaatccttct atctccatct ttggaacata tcatgggcac agatagatta ggaagagatg
      361 tttttgctag gattttgcaa ggtggtcaaa cttcacttat aataggattt ttagcggcat
      421 ctttttcatc ttttttagga cttattattg gaataactgc aggttatttt aaaggaaata
      481 ttgataaaac tattactatt gtaattgacc tatttttaac ttttccaaca ttttttcttc
      541 ttttagcttt agtttcatac atcgaagcaa atagtcttgt tttgatactt gtaatatcaa
      601 taactggttg gatgggaatg tcaagaatga taagaagtga aagttttgca cttagcaata
      661 agccatttat aaaaatatta aaaatagcaa atgtaagtaa aacaaaaatt attttaaaat
      721 attttgctcc acttttagct cctattttcc ttatctcttt ttcatttgga gttgcaggtg
      781 cgatattggc agaatctggt ctttcatttt tgggtcttgg agtaaatcca ccacaaatga
      841 gctggggaag ccttttaagt gatggaaaag ctgtaattga tattgcttgg catttaagct
      901 tttttccagg acttatgata tttataataa ctttttgcct aatccaaata agtgattatt
      961 tacaaaatat agcaaataaa aaagatttac ttaaaaatta ataaatacca aaatattgtt
     1021 taaatggatt tacaacaaaa ttttggtatt atccacacca taaattacca aaaataggaa
     1081 aaaattatga gtaaaaaaga tattaaaaaa gttgtacttg cttatagcgg tgggcttgat
     1141 acatctatta ttttaaaatg gcttcaagat gaatatgatg cagaggttat aacttttaca
     1201 gctgatttag gacaaggtga agaggttgaa cctgcacgtg cgaaagctat tgcttgtgga
     1261 attaaaccag aaaatgtata tattttagat gttaaagaag agtttgtaaa agattatgtt
     1321 ttccctatgt ttagagcaaa tgctatttat gagggtgagt atcttttagg aacaagtata
     1381 gcaagaccac tgattgcaaa aaaacttgta gaaattgcaa atgaaaaagg tgcacaagct
     1441 gtatctcacg gagcaactgg aaaaggaaat gaccaagtta gatttgaact tggagcctta
     1501 gctttaaatc cagatttaaa agtaattgca ccttggagag agtgggagtt aaactcaaga
     1561 gaatcactac ttgaatatgc aaaaaaacat ggaattgaaa tttctcaaaa gcatgttgat
     1621 gaaaatggaa acccaaaaat aagcccatat tcgatggatg caaacctttt acatatctct
     1681 tatgaag
//
LOCUS       DAOQAN010000302         1686 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_110226_length_1686_cov_2.112814, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000302 DAOQAN010000000
VERSION     DAOQAN010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110226_length_1686_cov_2.112814"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            17..430
                     /locus_tag="PKO10_05225"
     CDS             17..430
                     /locus_tag="PKO10_05225"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNW66580.1"
                     /translation="MKKAKVIINQYLKISALKVTFYSMLFCTITILIHSFFDSSLNIM
                     PLVNFNMWFYTIFLALVPTIISLLFLIKAIQLIGSTSASILGALEPLTAVLIGVYVFN
                     EKITFWLVIGIVFILFGVILIVLKDYIDKLFKIKN"
     gene            543..1199
                     /locus_tag="PKO10_05230"
     CDS             543..1199
                     /locus_tag="PKO10_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014474753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNW66581.1"
                     /translation="MKPLRYKYQTIEFGDNDIHLRTLKDKQQFSDKDKKAEDLGISSA
                     TWSLFGVVWPSSEVLANFIFDYDFKNKRILEVGCGIGLSSLILNRLNADITATDYHPE
                     AEKFLDINTELNKDDEIPFVRTCWSNEYQEELGKFDLIIGSDLLYERDHAVLLSNFIN
                     AHANDKAMVILVNPNRGYQSKFTKQMETFGFSFSFIEPKNTDFLEEPYKGKIFKYSRE
                     "
     gene            complement(1233..1430)
                     /locus_tag="PKO10_05235"
     CDS             complement(1233..1430)
                     /locus_tag="PKO10_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66582.1"
                     /translation="MAKNKGEPSLEKIDDYNGNESKQKRNTVRLIVILLLALGAFFAY
                     MKQPSNQTAIEIQEKTSIPAR"
     gene            complement(1446..>1686)
                     /locus_tag="PKO10_05240"
     CDS             complement(1446..>1686)
                     /locus_tag="PKO10_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HNW66583.1"
                     /translation="GVKKAQMALKDEAQKVTGYLLGGISPLGQKKLLRTVLDESINDF
                     KTIFISGGKRGLDIELNPKDLEVILKAKVGRIVAK"
BASE COUNT          605 a          215 c          226 g          640 t
ORIGIN      
        1 gatgattttg taaaaaatga agaaagcaaa agttattata aatcaatatc taaaaatatc
       61 tgcactaaag gttacttttt actctatgct attttgcaca ataactattt taatacactc
      121 attttttgat agcagtttaa atatcatgcc tttagtaaat ttcaatatgt ggttttatac
      181 aatattttta gctctcgttc ctacaatcat atctttactt tttttaataa aagcaattca
      241 gctaattggt tcaacaagtg catctatttt aggagcatta gagcctttaa ccgctgtact
      301 aattggagtt tatgttttta atgaaaaaat aactttttgg ttagttatcg gaatagtgtt
      361 tattttattt ggagttatac taatagtttt aaaagattat attgataaat tatttaaaat
      421 aaaaaactaa aatttgcttt tattttacta ttgcgatagt atgaattaga caattaaaat
      481 aaaagaggaa ttaatatgat aaaaaaaaat ttaaaaaagt tactaataat tagctcaata
      541 ttatgaaacc tcttcgttat aaatatcaaa caattgagtt tggtgataat gatatacact
      601 taagaacttt aaaagacaaa caacaattct ctgataaaga taaaaaagca gaagatttag
      661 gaatatcaag tgcaacttgg tctttatttg gagttgtttg gccatctagt gaagttttag
      721 caaattttat atttgattat gattttaaaa ataaaagaat tttagaagtt ggatgtggaa
      781 ttggtctatc aagccttatt ttaaatagat taaatgctga tattacagca actgactatc
      841 atcctgaagc tgaaaagttt ttagatatta acacagaatt gaataaagat gatgaaattc
      901 catttgtaag aacttgttgg agtaatgaat atcaagaaga acttggtaaa tttgatttaa
      961 ttattggaag cgatttatta tatgaaagag atcatgcagt tcttctttca aattttataa
     1021 atgctcatgc aaatgataaa gcaatggtta ttttagtaaa tccaaataga gggtatcaat
     1081 caaaatttac aaaacagatg gaaacttttg gtttttcatt tagctttata gaacctaaaa
     1141 atacagattt tttggaagag ccttacaaag gtaaaatatt taaatattca agagagtagt
     1201 agatacaaaa ttatgataaa agctaaaagc ttttatctag ctggaattga agtcttctct
     1261 tgaatttcaa tagctgtttg gtttgaaggt tgtttcatat atgcaaaaaa tgcacctaaa
     1321 gcaagaagta aaataacaat caatcttaca gtatttcttt tttgttttga ttcatttcca
     1381 ttataatcat caattttttc taaactcggt tcacctttgt tttttgccat ggtaactcct
     1441 tttaattatt tagcaactat tctacccact tttgctttta aaatcacttc taaatctttt
     1501 ggatttaatt caatatctaa tcctcttttt ccgccactta taaatatagt tttaaaatca
     1561 tttatactct catccaaaac cgttcttaaa agcttttttt gccctaaagg tgaaattcct
     1621 ccaagcaaat atccagttac tttttgtgct tcatccttta gagccatttg tgcttttttt
     1681 actcca
//
LOCUS       DAOQAN010000303         1681 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_110725_length_1681_cov_2.255843, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000303 DAOQAN010000000
VERSION     DAOQAN010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110725_length_1681_cov_2.255843"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /locus_tag="PKO10_05245"
     CDS             complement(<1..652)
                     /locus_tag="PKO10_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012148031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNW66584.1"
                     /translation="MKYANNVTELVGNTPLVKLQDASNVSGATVLGKCEFMNPSSSVK
                     DRIGTNMIKVALKEGLINKESTLIEPTSGNTGIALASVSAALGLKLVLVMPASMSIER
                     RRLLQALGAKLVLTPAEKGMKGAIEKANEIKDETPNSIILQQFQNASNPAIHRETTAQ
                     EILKDTDGKIDIFIAGVGTGGTISGVGKYLKEQNPNIKVVAVEPEGSPVISKNKKGS"
     gene            complement(737..1150)
                     /locus_tag="PKO10_05250"
     CDS             complement(737..1150)
                     /locus_tag="PKO10_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNW66585.1"
                     /translation="MPLISTKGVYGLAAIYELSKHKDDTPMQIKDISANASIPQNYLE
                     QLLSKLRRADLLISTRGARGGYLLAKSPKDIKIVDILIALEDDIKIVDSKVDNPILNL
                     FFEESKEKTKRIFDLTLADLDKYEGKYNEFLHYNI"
     gene            complement(1157..>1681)
                     /locus_tag="PKO10_05255"
     CDS             complement(1157..>1681)
                     /locus_tag="PKO10_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012148030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HNW66586.1"
                     /translation="TDVPLPNFCLRARLSLLRDIGATPAPFNSWLLIQSLETLSLRVE
                     KHSNNALKVAQFLQSHPKVKKVSYPGLKGDKYYDKAQKYFKNGLASGLISFEVSSFEE
                     AKKVIDSAKLFSIVVNIGDSKSLIVHPASTTHSQMNEEDLLKAGVTPVTVRLSIGLED
                     SADLIEDLNQALNK"
BASE COUNT          507 a          312 c          218 g          644 t
ORIGIN      
        1 gagatccttt tttattttta gatataactg gtgaaccttc aggttcaact gcaactactt
       61 ttatatttgg attttgctct ttaagatatt tacctactcc gcttatagtt ccgcctgttc
      121 caacaccagc tataaaaata tcaatttttc catctgtatc ttttaaaatc tcttgcgctg
      181 ttgtctctct atgtattgct gggttcgaag cattttgaaa ctgttgtaag ataattgaat
      241 ttggagtttc atcttttatc tcattagctt tttcaatagc acctttcatc cctttttcag
      301 caggagtaag aactaatttt gcacctaaag cttgaagaag tcttcttctt tcaatactca
      361 tagatgctgg cattacaagt acaagtttta atcctaaagc tgcactaacg cttgcaagtg
      421 caattccagt gtttccactt gttggctcta ttaaagttga ttctttattt ataagaccct
      481 ctttaagagc aacttttatc atatttgttc caattctatc ttttacactt gagcttggat
      541 tcataaattc acatttacct aaaactgtag ctccacttac attacttgca tcttgtagtt
      601 ttactaatgg agtatttcct actaattctg ttacattatt tgcatatttc atattattta
      661 cctttcaaaa ttattatatt aaactattaa attctctatg agaacgacct aaattgcttt
      721 tagaacaaag ccttgcttaa atattatagt gtaaaaactc attatatttt ccctcatatt
      781 tatctaaatc agctaaagtc aaatcaaaaa ttctttttgt tttctctttt gactcttcaa
      841 aaaatagatt taatatcggg ttatcaacct ttgaatcaac aatttttata tcatcttcaa
      901 gggcgattaa aatatcaact atctttatat cttttggact ttttgccaaa agatagccac
      961 ctctagctcc tcttgtactt attagtaaat cagcacgtct aagtttactt aaaagctgct
     1021 ccaagtagtt ttgaggaatt gaagcatttg ctgatatatc ttttatttgc atgggagtat
     1081 catctttatg tttgcttaac tcataaatag cagccaaacc atacaccccc tttgttgaaa
     1141 tcagtggcat atcaaactac ttgtttaatg cttgattcaa atcttcaatc aaatcagcac
     1201 tatcttcaag cccaatagat agtctaactg ttacaggagt aactccagct tttagtaaat
     1261 cctcttcatt catttgagag tgagttgtag aagctgggtg aacaattaaa gatttactat
     1321 ctccaatgtt tacaactatg ctaaacagtt ttgcactatc aataactttt ttagcctctt
     1381 caaaactact tacctcaaaa gatataagac cacttgcaag tccattttta aaatattttt
     1441 gtgctttatc ataatactta tcacctttta atcctgggta actaactttt tttacttttg
     1501 gatgtgattg taaaaactgc gcaactttta aagcattatt agagtgtttt tcaactctta
     1561 aacttaaagt ctctaaactt tgaattaaaa gccatgagtt aaatggtgca ggagttgctc
     1621 caatatctct taaaagtgat aacctagctc ttaaacaaaa gtttggaagt ggaacatctg
     1681 t
//
LOCUS       DAOQAN010000304         1666 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_112286_length_1666_cov_2.094351, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000304 DAOQAN010000000
VERSION     DAOQAN010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112286_length_1666_cov_2.094351"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="PKO10_05260"
     CDS             complement(<1..239)
                     /locus_tag="PKO10_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014473544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HNW66587.1"
                     /translation="MRELFRKIQPYTGKIFAVGLITFLSFLFYNLYNAPVDGFEEKFI
                     YLLKQYGYVILFVWSMLEGEMGLIMAGLMAHDGSM"
     gene            complement(248..1474)
                     /gene="trpB"
                     /locus_tag="PKO10_05265"
     CDS             complement(248..1474)
                     /gene="trpB"
                     /locus_tag="PKO10_05265"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HNW66588.1"
                     /translation="MSNYIPKKSIFDPNSNGEFGVDGEFGIFGGQYVPETLMPILKEL
                     EATYSKYRFDKEFWAEVNYYLKDYVGRENPLYFAKNISDELGAKIYLKREDLNHTGAH
                     KVNNVIAQGLLAKKMGKTKVIAETGAGQHGVATATIAALLGLECTVFMGAKDVQRQEL
                     NVFRMKLLGAKVVAVESGSKTLKDAMNDAIRYWVTNARDTFYIIGTVAGPHPYPMMVR
                     DFQAIIGYESRKQILEKENRLPDYVVACIGGGSNSIGMFSHFLEDKSVTCVGIEAGGL
                     GLDTPKHGSCLAKGTPGILHGQCSYLLQDEDGQVLEAHSISAGLDYPGVGPEHSFHKD
                     NKTVTYDNITDKEALDAFVWLSQKEGIIPAFESAHAIAYLKKAKDKLKDKLVIVCLSG
                     RGDKDMIQAKSLLNFD"
     gene            complement(1471..>1666)
                     /locus_tag="PKO10_05270"
     CDS             complement(1471..>1666)
                     /locus_tag="PKO10_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005021649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HNW66589.1"
                     /translation="DIKKPKVLLMDDLVVSGGTAVAAANLIEKLDASLIEACFILNFS
                     ILPGKAKLEEKTPVYAVLEI"
BASE COUNT          528 a          352 c          188 g          598 t
ORIGIN      
        1 ttcatagagc catcatgagc cataagacca gccataatta aacccatctc accttctagc
       61 atactccata caaaaagtat cacataacca tactgtttta aaagataaat aaatttctct
      121 tcaaacccat caacaggagc attgtataga ttataaaaaa gaaaagataa aaatgttatc
      181 aatccgactg caaaaatctt tcccgtatat ggctgaattt tcctaaaaag ttctctcatt
      241 tttatcctta gtcaaaattt agtaggcttt ttgcctgtat catatcttta tctcctctac
      301 ctgataaaca cactattact aatttatctt ttaatttatc ttttgctttt tttaaatatg
      361 caattgcatg agctgattca aatgctggaa taattccctc tttttgactt aaccaaacaa
      421 aagcatcaag tgcttcttta tcagttatat tatcatatgt aactgtttta ttatctttgt
      481 gaaaactatg ttctggtcca actcctggat aatcaagacc tgcactaata ctatgggctt
      541 ctaaaacttg tccatcttca tcttgaagta aatatgaaca ctgtccatgt aaaattccag
      601 gagttccttt tgctaaacag cttccatgtt taggcgtatc aagtcctaat ccccctgctt
      661 caattccaac acaagtaaca cttttatctt ctaaaaaatg agaaaacata ccaattgagt
      721 ttgatcctcc accaatacat gcaactacat aatcaggaag tctattctct ttttctaaaa
      781 tctgttttct tgattcataa ccaataattg cttgaaaatc tcgcaccatc ataggatatg
      841 gatgaggacc tgcaactgtt ccaattatat aaaaagtatc tcttgcattt gtaacccaat
      901 atctaatcgc atcattcatt gcatctttta aagttttgct tccactttct actgcaacaa
      961 cttttgcacc taaaagtttc attctaaata catttaactc ttgtctttgt acatcttttg
     1021 cacccataaa aacagtacac tcaagtccaa gcaaagctgc aattgttgca gttgcaactc
     1081 catgttgccc agctcctgtt tcagctatta cttttgtttt tcccattttt tttgctagta
     1141 atccttgagc tattacatta ttaactttat gagctcctgt gtgatttaaa tcttctcttt
     1201 ttaaatagat ttttgcccca agttcatcac ttatattttt tgcaaaatat agtggatttt
     1261 ctcttccaac ataatctttt aagtagtagt ttacctcagc ccaaaactct ttatcaaatc
     1321 tatatttact ataggtagct tcaagctcct ttaaaatagg catcaatgtt tcaggaacat
     1381 attgtcctcc aaaaattcca aactcaccat caacaccaaa ctctccattt gaatttggat
     1441 caaatatact tttttttgga atatagttac tcatatttct agcaccgcat aaacaggagt
     1501 tttctcttca agttttgctt ttccaggcaa tatagaaaaa ttcagaataa aacaagcttc
     1561 aattaaagag gcatctaatt tctcaattaa attagctgct gcaactgctg taccaccact
     1621 tacaactaaa tcatccatta aaagaacttt tggttttttt atatct
//
LOCUS       DAOQAN010000305         1655 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_113445_length_1655_cov_3.063750, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000305 DAOQAN010000000
VERSION     DAOQAN010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113445_length_1655_cov_3.063750"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1226)
                     /gene="gdhA"
                     /locus_tag="PKO10_05275"
     CDS             complement(<1..1226)
                     /gene="gdhA"
                     /locus_tag="PKO10_05275"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014667426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HNW66590.1"
                     /translation="MANINELLEYLKRTSPGQDEFHQAAEEVLHSLEPLFEKYPKYKE
                     NKVLERLVEPERQIMFRVTWVDDKGEIQINKGYRIQFSSTLGPYKGGLRFHPSVNTGI
                     IKFLGFEQIFKNALTGLQIGGGKGGSDFDPKGKSDNEIMAFCQAFMIELYRHIGATTD
                     VPAGDIGVGGREIGYMFGMYKKLANTYDGTLTGKSLKWGGSLARTEATGYGCVYFAKN
                     MLESRGESLKGKKCTVSGSGNVSIYTIEKLYHLGALPITCSDSSGMILDEEGIDLDLL
                     KELKENQRARLTEYVKYRKNAKYIPVSDYPKGRNAVWSVPCFAAFPSATQNELNLEDA
                     KELIKNGCVCVSEGANMPSTNEAVDFFVAQKIAYGPGKAANAGGVATSQLEMAQNASM
                     VSWTFEEVDAKLEKIM"
     gene            complement(1364..>1655)
                     /locus_tag="PKO10_05280"
     CDS             complement(1364..>1655)
                     /locus_tag="PKO10_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HNW66591.1"
                     /translation="TGSKMDEVIFEEFKGTGNSEVVLSRNASNKRVYPAIDIVKSGTR
                     KEELLLTPDILQKTWILRNAMSEMDEVDVLKFLYPKMQKTKNNDDFFASMNE"
BASE COUNT          495 a          314 c          200 g          646 t
ORIGIN      
        1 ttcataattt tttctagttt tgcatcaacc tcttcaaaag tccaagaaac catagaagca
       61 ttttgtgcca tctctagttg acttgtagca actccaccag cgtttgctgc ttttcctggt
      121 ccatatgcaa ttttttgtgc aacaaagaaa tcaacagctt catttgttga aggcatattt
      181 gcaccttcgc ttacacaaac acaaccattt ttaattaatt ctttagcatc ttctaaattt
      241 aattcatttt gtgttgcact tgggaatgct gcgaaacatg gaactgacca aactgcattt
      301 ctaccttttg gataatctga aactggaata tattttgcat tttttctata ttttacatac
      361 tcagttaatc tagctctttg gttctctttt aactctttta aaaggtctaa atcaatacct
      421 tcttcatcta aaatcattcc agatgaatcg ctacaagtaa ttggtaatgc acctaagtga
      481 tatagttttt caatagtata aatagataca tttccagaac cagaaactgt acatttttta
      541 ccttttaaac tctctcctct agattcaagc atattttttg caaaatatac acaaccataa
      601 ccagttgctt cagttcttgc aagtgatcca ccccatttta gagattttcc tgttaaagtt
      661 ccatcataag tgtttgcaag ttttttatac ataccaaaca tatatccaat ttctcttcca
      721 ccaacaccaa tatctccagc tggaacatct gtagttgctc caatgtgtct gtaaagttca
      781 atcatgaaag cttgacaaaa cgccattatt tcattatctg atttaccttt tggatcaaag
      841 tcacttccac cttttcctcc accaatttga agacctgtta aagcattttt gaatatttgt
      901 tcaaacccta aaaatttaat aattccagtg tttacacttg ggtgaaatct taatccacct
      961 ttgtatggcc ctaaagtcga actaaattgt attctataac ctttgttgat ttggatttct
     1021 cctttatcat caacccaagt aactctaaac attatttgtc tttcaggttc aactaatctt
     1081 tctaaaactt tattctcttt atattttgga tatttttcga atagtggttc tagtgaatgt
     1141 aaaacctctt cagcagcttg atgaaactca tcttgtccag gacttgttct ttttagatat
     1201 tctaaaagtt cgtttatatt tgccattttc cttcctttta ttcaaatttc gggaaaggag
     1261 tttatcagaa gaattatttg attttgttta aatttaggta aaatctttac aaatttataa
     1321 aaattttacc ttttttctca tttttaagaa gatattaagt aatttattcg ttcattgaag
     1381 caaaaaagtc atcattattt tttgtttttt gcatttttgg atataagaat tttaaaacat
     1441 ctacttcatc catttcactc atagcatttc ttaaaatcca agttttttgt aaaatgtcag
     1501 gagttaaaag aagctcttct tttctagttc ctgattttac aatatcaata gcaggataaa
     1561 ctcttttatt tgatgcattt ctactaagaa ctacttcaga gtttccagtt cctttgaact
     1621 cttcaaaaat aacttcgtcc atttttgaac cagta
//
LOCUS       DAOQAN010000306         1645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_114532_length_1645_cov_3.432075, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000306 DAOQAN010000000
VERSION     DAOQAN010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114532_length_1645_cov_3.432075"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="PKO10_05285"
     CDS             complement(<1..216)
                     /locus_tag="PKO10_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66592.1"
                     /translation="MAQVNLGRIGFVNKGSWNNVANYKLNDVVKYGGSIYAAKRPNTG
                     ITPPTNGTDTDDWFYFVSNSNSDYVNKT"
     gene            complement(231..617)
                     /locus_tag="PKO10_05290"
     CDS             complement(231..617)
                     /locus_tag="PKO10_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66593.1"
                     /translation="MINKITQDISPITALPDLLSENRPAAVNLLVEVELPRNSIEMKN
                     LVGQLNTFKTEVNATGDEVKANANEVKANANEVKANMEQIDRLAYSLTQMPITMSAVI
                     DGELIMYYNDPIQSLSLVDGDLIVNY"
     gene            complement(610..>1645)
                     /locus_tag="PKO10_05295"
     CDS             complement(610..>1645)
                     /locus_tag="PKO10_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66594.1"
                     /translation="KIYKARQTISLPTYYVYNKEEKALYIPSKDKFAETATFNPTSEF
                     ENRFIFLDDTDTLYKYKGSTEITLDPTTINFEDTLLWENQGKQLNGYLVELSYPDVSP
                     LYWEDMGYVNTRRAFDESSSSQTISDLNEDLVFTFQTGNVDRIALFGVSAKEIKIKVK
                     LGIQPESPENTIEQIIPLYTQTGEHFYEILTSMIVFKKTYWLKIPTSLAQTITLTFVN
                     ETYTRVRVGDITLGRGKILGATLDSVNYDIKDNSTYGSDTGRSDSYIEGGYRKINDFT
                     VSYLTRDTSTVLKSLGSLRGKITVFNLSNEENRDFLRIKGFMRTRPLNYISNSRKSKI
                     NIKVEGRLND"
BASE COUNT          436 a          258 c          228 g          723 t
ORIGIN      
        1 tgttttattc acatagtcac tattactatt gcttacaaaa taaaaccaat catctgtatc
       61 tgttccgttt gttggtggag ttatacctgt atttggtctt ttagctgcat aaatactgcc
      121 accatattta actacatcat ttagcttgta atttgctaca ttattccagc ttcctttatt
      181 tacaaatcct attctaccta aatttacttg tgccatatat tttccttttt ttaataattt
      241 actatcaaat cgccatctac tagagatagt gattgaattg ggtcgttgta atacattata
      301 agctcaccat ctattactgc tgacattgtg attggcattt gagttaagct atatgctaat
      361 ctatctattt gttccatatt tgcttttact tcatttgcat ttgcttttac ttcatttgca
      421 tttgctttta cttcatcgcc tgttgcattt acttctgttt taaaagtatt tagctgtcca
      481 actagatttt tcatctctat tgaatttctt ggtagctcta cctcaacaag tagatttact
      541 gctgctggtc tattttcaga taataaatct ggtaatgctg ttattggact tatgtcttgt
      601 gttattttat taatcattta atcttccttc tactttgata tttattttag attttcttga
      661 atttgatata tagtttaaag gtcttgttct cataaatcct tttattctaa gaaaatctct
      721 attttcttca ttacttagat tgaaaactgt tatttttcct cttaaagagc ctagtgattt
      781 aagtacagtt gatgtatctc ttgtaagata tgaaactgta aaatcgttta tttttctata
      841 tccaccttca atatatgaat ctgaacgacc tgtatctgaa ccataagttg agttatcttt
      901 tatatcataa tttacagagt ctagtgttgc acctagtatc tttcctcttc caagtgtaat
      961 atctcctact cttactcttg tatatgtttc atttacaaat gttagagtga ttgtttgtgc
     1021 taaagatgta gggattttta accaatatgt ttttttaaat acaatcatag atgttagaat
     1081 ttcgtaaaag tgttctcctg tttgagtata tagaggtatt atttgctcta ttgtattttc
     1141 tggactttct ggttgaattc ctagttttac ttttattttt atctcttttg ctgatactcc
     1201 aaaaagtgct attctgtcta catttccagt ttgaaaagta aatactaaat cttcatttaa
     1261 gtctgaaata gtttgacttg aagatgattc atcaaaagct cttcttgtgt ttacatatcc
     1321 catatcttcc cagtatagtg gagatacatc tggataagat aattctacta aatatccatt
     1381 tagttgtttt ccttggtttt cccatagtag agtatcttca aaattaattg ttgttgggtc
     1441 tagtgttatt tctgttgaac ctttgtattt gtatagagta tctgtatcat ctaaaaagat
     1501 aaatctattt tcaaattcac ttgttggatt aaatgttgct gtttctgcga atttatcttt
     1561 agatgggata tatagtgctt tttcttcttt attgtacaca taatatgttg gcagagatat
     1621 tgtttgtctt gctttgtata tctta
//
LOCUS       DAOQAN010000307         1641 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_115042_length_1641_cov_1.911097, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000307 DAOQAN010000000
VERSION     DAOQAN010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115042_length_1641_cov_1.911097"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..1200
                     /locus_tag="PKO10_05300"
                     /pseudo
     CDS             1..1200
                     /locus_tag="PKO10_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014475195.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
     gene            1197..>1641
                     /locus_tag="PKO10_05305"
     CDS             1197..>1641
                     /locus_tag="PKO10_05305"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014469253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNW66595.1"
                     /translation="MSKVVVITGGGTGGHLKIADVFISEFKKRGFEVVFIGSSYGQDK
                     FWFENDDRLKEKIFLNTSGVVNKRGIAKIFSLLNIFYKAIFCLKILKKYRVQKVVSVG
                     GFSAAAASLATIFKKDCNLYLHEQNSKIGALNKITLKYTKKAYSSF"
BASE COUNT          532 a          194 c          282 g          633 t
ORIGIN      
        1 ttgtttattt tggtttctat gctcattatt gtaagcatag ttttttcata ttcgcttact
       61 atttatactg ttgagttttt aggttatgga cagtttcact atattttaag acaaggttta
      121 gttgggatat tttgtatata cctaatgtgg tggatggcaa gattaaatcc taataaaata
      181 atgcatagaa catgaatgac actatttggt gttggacttt tcttaatggt cataatgcct
      241 tttctaccag cttctttagt tactgcatca ggtggtgcaa atagatggat taggcttcct
      301 ggaatatctt tatctcctgt tgagatattt aaaataggtt ttatatattt tttatcttgg
      361 tcatttttta gaaaagttat tcatcaacca aagaaaggtc tctttgggga tttattacta
      421 ctttctccat actttttagt atttattttt attgtttttt tgattgctgt tttacaaaaa
      481 gatttgggac aagttgcact actaactatt attatgctta ctttaattat atttgcaaat
      541 agatctttaa aaattatctt tgcattaatt cttatagcta ttgtaggtat gatagttcta
      601 atagcagcag cacctcatag aataaacaga attcactctt ggtggtcaat ggttcaagat
      661 ggtattttat cagttcttcc atcaacattt gagcagtatt tgagaataaa aaacttacca
      721 gaaccatatc aagtatcaca ctctttaaat gctattcaca atggtggttt ttttggacaa
      781 ggtatctctt tgggagattt aaaagttgga tttttaagtg aggttcacac agacttcgtt
      841 attgctggaa taactgaaga gataggttgg cttggaatat ttgttataac tacaatttta
      901 atgctagtta ttttaagaat tttagcaatt agtagaaaag ttgatagtaa aatttttcat
      961 ctttttacga caggaattgc tcttatgatt attgttgcat ttttaataaa tggtgctgga
     1021 atttcaggaa ttattccaat taaaggtatt gccgttccat ttttatcata tggtggttca
     1081 tctttaatta cgagctcatt tgctatagga ttagttcttt caattagtag aactgtaaaa
     1141 aagtatgaac cacaaaaaag tgttataaaa gaaaaaccta aaaggataat tattagatga
     1201 gtaaagttgt tgtaataaca ggtggaggaa ctggaggtca tctaaaaatt gctgatgttt
     1261 ttatatctga atttaaaaaa agaggttttg aagttgtttt tataggttca agctatgggc
     1321 aagataagtt ttggtttgaa aatgatgata gattaaaaga gaagattttt ttaaatacaa
     1381 gtggagttgt gaataaaaga ggaatagcta agattttttc acttttaaat atattttaca
     1441 aagctatctt ttgtcttaaa attttaaaaa aatatagagt acaaaaggtt gtatctgttg
     1501 gtggatttag tgctgcagca gcttcacttg caactatatt taaaaaagat tgcaatctat
     1561 atttgcatga acaaaactca aaaattgggg ctttaaataa aatcactcta aaatatacaa
     1621 aaaaggccta ctcatctttt c
//
LOCUS       DAOQAN010000308         1640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_115168_length_1640_cov_1.476972, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000308 DAOQAN010000000
VERSION     DAOQAN010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115168_length_1640_cov_1.476972"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1640)
                     /locus_tag="PKO10_05310"
     CDS             complement(<1..>1640)
                     /locus_tag="PKO10_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66596.1"
                     /translation="FLNKFIEIINKIYTTQTTKANALADLNSALMEAIQLNTQIDIGM
                     KVKQDSYSQYNAFGLGTKLNNTNEWLKDGQEGIVNKISNFATSDKVRDKAEYASNFIE
                     NISNWRHFQFIRDSRIISDTVTDIVEDTTKESTSWFYEAVRKIKNNRDKDKVDFAAVV
                     RNKLGDALKHFEKEEREAMTFMIQGDWKALNVSIDEYKDLLQDNDLVDEKINELKTKI
                     GLNEYNNQAALLGYYIVTGEAKGSALMKNAYQIYHRFHTGKQTSPLKNSMSDDENISA
                     IDELASLYAIKYTENHIKENIVNAINRDSESVKMASDIHYGYKAKENSGQLGRFGKFA
                     DKGYVRKSGKVKMKFDIIPESKLIDKSWFNFLKHNIVRERPDIQELMGSSEKYYMVID
                     RNYDTARTQGALDDISIIDHGSDLGSFTAGKDLDFDARNALRKKRFFKDKTYLNFAQD
                     DSIEAMRARENNSVAEFNIYGEIDSYSEPISEADLINHGRVINDVADVIANTTSHIES
                     KEKALINNQRFIDLLIEDSEKNIGAPGYVFLSPDSSEK"
BASE COUNT          473 a          306 c          192 g          669 t
ORIGIN      
        1 tctttttcgc tagaatctgg agataaaaat acatatcctg gagcacctat atttttttcg
       61 ctatcttcta tcaataaatc tataaatctt tgattgttta ttaaagcttt ctctttactc
      121 tctatatgag atgtagtatt tgctataaca tcagcaacat catttattac tctaccatga
      181 tttattaaat cagcttcgct tattggttca ctataagaat ctatttctcc atagatgttg
      241 aattcagcaa cactgttatt ctctctggct ctcatagctt ctatgctatc atcttgagca
      301 aaattcagat atgtcttgtc tttgaaaaat cttttttttc ttagtgcatt tcttgcatca
      361 aaatccaaat ctttcccagc agtaaaactt cccaaatcag aaccatggtc tataatagaa
      421 atatcatcaa gtgctccctg agttcttgct gtatcgtagt ttctatctat aaccatatag
      481 tatttttcgc tactacccat tagctcttga atgtcaggtc tttctctaac gatattatgt
      541 tttaagaaat tgaaccaact tttgtctatc aatttgcttt ctggaataat gtcaaatttc
      601 attttaactt taccagattt tctaacatat cctttgtctg caaatttacc aaatcttcct
      661 agctgaccac tattctcttt cgctttgtat ccataatgaa tatcgctagc catcttaaca
      721 ctttcagaat ctctatttat agcatttact atgttttctt ttatatgatt ttcagtgtat
      781 tttattgcat ataaagaagc aagctcatct attgcagata tattttcatc gtcactcata
      841 gagtttttca aaggagaagt ttgctttcca gtatgaaatc tgtgatatat ttgatatgca
      901 tttttcatca acgcagaacc ttttgcttct ccagtaacta tatagtaacc aagtaaagca
      961 gcttgattgt tatattcatt tagtcctatt tttgtcttca attcgtttat tttctcatca
     1021 accaaatcat tatcttgaag taaatcttta tactcatcta tagaaacatt taaagccttc
     1081 caatctcctt gaatcataaa agtcatagct tctctttctt ctttttcaaa gtgtttcaat
     1141 gcatcaccaa gtttgtttct aacaactgct gcaaaatcaa ctttatcttt atctctattg
     1201 tttttaatct ttcttacagc ttcgtagaac caagaagttg actcttttgt tgtatcttca
     1261 acaatatcag taacagtatc tgaaataatc ctagaatctc tgatgaactg aaaatgtctc
     1321 caatttgata tattttcaat aaagttagat gcatattctg ccttatctct aactttgtct
     1381 gatgtcgcaa aatttgatat tttatttact ataccttctt gcccatcttt tagccactca
     1441 tttgtgttat ttaatttagt tcctagtcca aatgcattgt attgagaata agaatcttgt
     1501 tttactttca ttcctatatc tatttgagta tttaattgaa tagcttccat cagagcagag
     1561 ttcaaatcag ctaatgcgtt tgccttagtt gtttgagttg tatatatttt attgataatc
     1621 tcaataaatt tatttaaaaa
//
LOCUS       DAOQAN010000309         1635 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_115710_length_1635_cov_2.195570, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000309 DAOQAN010000000
VERSION     DAOQAN010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115710_length_1635_cov_2.195570"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(55..>1635)
                     /locus_tag="PKO10_05315"
     CDS             complement(55..>1635)
                     /locus_tag="PKO10_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HNW66597.1"
                     /translation="KYLEVIGKKLAIDTLDKIKSTLPLEAIVVKDGKKETKALNLVEV
                     GDFIELKIGEKVPVDGKIISGNASFDESSLTGESIPVYKKTGDNIFSGTVILDSTILF
                     EVVKDFKNSTFSSIVTLLEDSLNSKPKIQTLANKLSRGFSLIILSIAFITFLVWYYFG
                     LDLGFYFEGVNQFERSFITAISVVVIACPCALALATPMASLVGISELAKKSLLFKEAK
                     FIETIANATTVVFDKTGTLTKGELEVGFVEFFNKDEKSMNLLYSLLDSSNHPVSIAVK
                     RYIKENFEVSNLILEDVKNIEAKGLSARYENIEILGGNEALLKEFDVNINIDLNSKFT
                     QYLFCIDKKIVANFELKDELKDDAKELIEYLKEQNIESIMLTGDNNFVASSIAKELEI
                     SNYKANLTPKDKADFIKDLKNSGKTVVMVGDGVNDSVALASSDVAIAMGNSADVSMMV
                     SDVVMLNSKLKSLKDAFIISKKTYKHIKQNLAFSLIYNTITIPIAAAGFIIPLFAALS
                     MSLSSLVVVLNSLRIKLK"
BASE COUNT          581 a          278 c          173 g          603 t
ORIGIN      
        1 ccaactacta acataaaaaa tagtgtatca tcaatcataa tataaatttt tcctttattt
       61 gagttttatt ctaagagagt tcaaaacaac tactaatgaa cttaaactca tagataatgc
      121 agcaaaaagc ggaattataa atccagctgc tgcgattgga attgtaattg tgttataaat
      181 taaagagaaa gctaaatttt gttttatatg tttataggtt tttttagaga ttataaaagc
      241 atcttttaat gattttagtt ttgaatttag cattactaca tcagaaacca tcatagaaac
      301 atcagcagaa tttcccatag caattgcaac atcactagaa gctagtgcaa cactatcatt
      361 tacaccatct ccaaccataa caacagtttt accactattt tttaaatcct ttataaaatc
      421 tgctttatct tttggagtta gatttgcttt ataatttgat atttccaact cttttgcaat
      481 acttgaagct acaaaattat tatctcctgt taacattata gattctatat tttgttcttt
      541 taaatactct ataagctctt tcgcatcatc ttttaactca tcttttaact caaaatttgc
      601 aactattttt ttatctatac aaaatagata ttgagtaaat tttgagttta aatcaatgtt
      661 tatatttaca tcaaactctt ttaaaagagc ctcatttcca cctaaaattt caatattttc
      721 atatcttgca cttaatcctt ttgcttctat atttttaaca tcttctaaaa ttagattaga
      781 gacttcaaag ttctctttaa tatatctttt tacagcaata ctaacaggat gattcgagct
      841 atcaagcaaa gagtaaagaa gattcatact tttttcatct ttattaaaaa actctacaaa
      901 accaacttct aattcacctt ttgtcaaagt tcctgtttta tcaaaaacaa cagttgtagc
      961 atttgcaata gtttctataa attttgcctc tttaaatagt aaggattttt ttgccaactc
     1021 actaattcca actaaacttg ccattggtgt tgcaagtgct aaggcacaag gacaagcaat
     1081 tacaacaact gaaatagcag ttataaatga cctttcaaac tgatttacac cctcaaaata
     1141 aaaacctaaa tcaagcccaa aatagtacca aactaaaaat gtgataaatg caatacttaa
     1201 aataattaaa ctaaatcctc ttgagagctt atttgccaaa gtttggattt taggtttaga
     1261 atttagtgaa tcttcaagca aagttacaat tgatgaaaaa gttgagtttt tgaaatcttt
     1321 tacaacttca aaaagtattg tagagtctaa aataacagtt ccactaaaaa tattatctcc
     1381 tgttttttta taaacaggta ttgactcacc agttaagctt gattcatcaa aagatgcatt
     1441 tccgcttata atttttccat caactggaac tttctctcca atcttaagct caataaaatc
     1501 accaacttcc actaaattta aagcttttgt ctcttttttc ccatctttta caacaatagc
     1561 ttctaaagga agagtagatt ttattttatc taaagtatca atagctaatt ttttaccaat
     1621 tacttcaaga tattt
//
LOCUS       DAOQAN010000310         1632 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_116082_length_1632_cov_2.018389, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000310 DAOQAN010000000
VERSION     DAOQAN010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116082_length_1632_cov_2.018389"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(129..1109)
                     /locus_tag="PKO10_05320"
     CDS             complement(129..1109)
                     /locus_tag="PKO10_05320"
                     /EC_number="1.4.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005870674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047850 - diaminopimelate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate dehydrogenase"
                     /protein_id="HNW66598.1"
                     /translation="MNNQIKVAIVGYGNLGRGVELSISKNPDMSLVAVFSRRDPKSVT
                     TINTPVFSLENILDFKEKIDVLILCGGSKDDLPIQGPKFAEHFHIVDSYDNHAEIPAY
                     FASVDKACQKNKKIGMISVGWDPGMFSINRIYGEALLPDGDTYTFWGKGLSQGHSDAI
                     RRVEGVKNGVQYTIPSNSAIEKVRSGARPTLTTKEKHSRECFVVLKDGADAKKVENEI
                     KTMPNYFEPYDTTVNFISQEEFDKNHNTMPHGGFVIRSGKSSEGVNQVIEYALKLDSN
                     PEFTASVLVAYARATYKMSLKGDFGAKTVFDVAPILLSSKSSADLVKELL"
     gene            1256..>1632
                     /locus_tag="PKO10_05325"
     CDS             1256..>1632
                     /locus_tag="PKO10_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNW66599.1"
                     /translation="MHIIEFENIDVGYDEKVVLKDINLKIKSGEHFAILGANGSGKST
                     LMKLIQSQIHPRQTKNFKKEIFGKSRYSIFELKKELGVITNDLHNYFEKEGGYLSGFE
                     VVLSGYYSSIGIFTHQDFTKEQI"
BASE COUNT          567 a          249 c          219 g          597 t
ORIGIN      
        1 aatatagcat tttgttgagc attgttatac caatatactc attatattta tttttataca
       61 attcttttta tttatatttt gtttgtatca tattatcatc tgttaaaggt tcaaaattta
      121 aaataatatt ataaaagttc ttttactaaa tcagcagatg attttgaaga tagtaaaatt
      181 ggtgctacat caaatactgt tttagctcca aaatcacctt ttaaagacat tttatatgta
      241 gctctagcat atgctacaag tacacttgca gtaaactctg gattgctatc aagttttaaa
      301 gcatattcaa ttacttgatt tacaccttcg cttgattttc cacttcttat tacaaatcca
      361 ccatgaggca tagtattgtg atttttatca aactcttctt ggcttataaa atttactgtt
      421 gtatcataag gctcaaagta gtttggcatt gtttttatct cattttctac ttttttagca
      481 tctgcaccat cctttaaaac cacaaaacac tctctagaat gtttttcttt tgtagttaaa
      541 gtaggtcttg caccacttct tactttttca atagcactat ttgaaggaat tgtatattga
      601 actccatttt ttacaccctc aactcttcta atagcatctg agtgtccttg acttaatcct
      661 tttccccaaa atgtataagt atctccatct ggaagtaaag cttctccata tattctattt
      721 attgaaaaca ttccaggatc ccaaccaaca gaaatcattc ctattttttt atttttttgg
      781 caagctttat caacacttgc aaaataagct ggaatttcag catgattatc ataactatct
      841 actatatgaa aatgctccgc aaattttggt ccttgaatag gtaaatcatc ttttgaacca
      901 ccacataaaa ttaaaacatc gatcttttct ttgaaatcta aaatattttc taaggaaaat
      961 acaggagtgt ttattgtagt tacacttttt gggtctcttc ttgaaaaaac cgctactaaa
     1021 ctcatatcag gattttttga aatagaaagc tcaacacctc ttcctaaatt cccatatcca
     1081 acaattgcaa ctttaatctg attattcatg ctaaacgctc cttttgaagt agtaagtttt
     1141 tttggtatgt aagatataac aaaaaaattt cggcaatatt ttactattaa aaaacttaat
     1201 aaaatagtta gtagtgttgt tgtagaatga cgattgtcca attttaggag taaaaatgca
     1261 tataatagag ttcgaaaata tagatgttgg atatgatgaa aaagttgttt taaaagatat
     1321 aaatttgaaa attaaaagtg gtgaacactt tgcaatttta ggtgcaaatg gaagtggaaa
     1381 atcaacttta atgaaactaa tacagtctca aatccatcca agacagacaa aaaatttcaa
     1441 aaaagagatt tttgggaaaa gtagatactc catttttgaa ttaaaaaaag agttaggagt
     1501 aataacaaat gacttacaca actattttga aaaagaaggt ggatatttaa gtggttttga
     1561 ggtagtttta agtggatatt atagctcaat aggaatattt actcatcaag attttacaaa
     1621 agagcagata aa
//
LOCUS       DAOQAN010000311         1622 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_117235_length_1622_cov_2.064454, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000311 DAOQAN010000000
VERSION     DAOQAN010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117235_length_1622_cov_2.064454"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..907)
                     /locus_tag="PKO10_05330"
     CDS             complement(<1..907)
                     /locus_tag="PKO10_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66600.1"
                     /translation="MLISNGSALNILLANNNRVLNDALKEADNKTLNNLLKQDSSNSS
                     SSTNTAASSILKEVLNSIKDGTKSNSSLENILKNSTAFKDLGNLSSNLSSLLESIKDD
                     ESLQKFKPLLENFLKNIKDVTADNLKEQIKNSGIFLENKLSSNPNAKLENLLQNISNL
                     LKTIDTPEAKNSSQNIDNILKNIPKDGSLKGSELLNNLKTLVSSLQNLSSSLNSNQTQ
                     TLNNLANELKNFIQNGSMIESKTENLVQKTLTQTQTSETENINSANNNIKSLINNQVK
                     ELLNQIKQDLTQNQNIIQNKNILPLI"
     gene            complement(950..>1622)
                     /locus_tag="PKO10_05335"
     CDS             complement(950..>1622)
                     /locus_tag="PKO10_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoid-structuring protein H-NS"
                     /protein_id="HNW66601.1"
                     /translation="RSLKEELRPKNESVVDMIRIATYIHQIPAAIELIEDAHAKGYET
                     TVNIMAISKVSEKELDEVLEALSKTNVDVIYIADSFGSFYPEQMRDLAGKYLGFAEKS
                     GKKVGIHAHNNLQLAYANTLEAMIYGASFLDVTVSELGRGAGNCPLELLLGFLKNPKY
                     KQMPVLEFIENYIVDLEKKLDWGYSIPYMITGQLNEHPRAAMKARDEGDTKYREFFKN
                     ISFME"
BASE COUNT          487 a          226 c          204 g          705 t
ORIGIN      
        1 caatcaaagg caaaatattt ttattttgta ttatattttg attttgtgtt aaatcttgtt
       61 ttatttgatt taaaagctct tttacttggt tattaatcaa tgatttaata ttattattgg
      121 cactatttat attttcagtt tctgaggttt gtgtttgcgt taatgttttt tgaactaaat
      181 tttcagtttt tgattctatc attgacccat tttgtatgaa attttttaat tcatttgcaa
      241 gattatttaa agtttgtgtt tgattagaat ttaaagagct acttagattt tgtaaggaac
      301 ttactaatgt tttaagatta tttagtaact cactaccttt taaagagcca tcttttggta
      361 tattttttaa aatgttatca atattttgac tgctattttt tgcctcaggc gtatctatag
      421 tttttaaaag atttgagata ttttgcaaaa gattttctag ttttgcattt ggatttgaag
      481 ataacttatt ttctagaaaa ataccactat tttttatctg ttcttttaaa ttatcagcag
      541 ttacatcttt tatatttttt aaaaaatttt ctaaaagtgg tttaaatttt tgtaaagatt
      601 catcatcttt aatactttct aataaagatg ataaatttga agatagattt cctaaatctt
      661 taaaagcagt tgagtttttt aagatatttt ccaaagagct atttgatttt gttccatctt
      721 taattgagtt taaaacctct tttaaaatag aactagcagc tgtatttgta gagcttgaag
      781 agtttgaact atcttgtttt aaaagattat ttagagtttt attatctgcc tcttttaaag
      841 catcatttaa aactctatta ttgtttgcta atagtatatt tagtgctgat ccatttgaaa
      901 ttagcatttt agaccataat attatatgtt tttttagagt agattttatc tactccataa
      961 aagagatatt tttaaaaaac tctctatatt ttgtatcacc ttcatctctt gctttcatag
     1021 cagctcttgg gtgttcattt aattgacctg ttatcatata aggaatacta tatccccagt
     1081 caagtttttt ctctaaatca acaatataat tttctataaa ctctaaaaca ggcatctgtt
     1141 tatattttgg attttttaag aaccctaaaa gaagctctag cggacaattt ccagctcccc
     1201 ttccaagttc tgaaactgta acatctaaaa agctagctcc ataaatcata gcttctaaag
     1261 tgtttgcata tgcaagttga agattattgt gtgcatgaat tccaactttt ttacctgatt
     1321 tttctgcaaa acctaggtat tttccagcta aatctctcat ttgttctgga taaaatgatc
     1381 caaaactatc agctatataa attacatcaa catttgtttt acttaaagct tctaaaactt
     1441 catctaattc tttttctgat acttttgaaa ttgccattat atttacagtt gtttcgtaac
     1501 cttttgcatg tgcatcttct attagttcaa ttgctgctgg gatttggtga atatatgttg
     1561 cgattctaat catatcaaca acactctcat tttttggtct aagctcctct tttaaacttc
     1621 tt
//
LOCUS       DAOQAN010000312         1618 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_117695_length_1618_cov_2.529750, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000312 DAOQAN010000000
VERSION     DAOQAN010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117695_length_1618_cov_2.529750"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            21..686
                     /gene="uppS"
                     /locus_tag="PKO10_05340"
     CDS             21..686
                     /gene="uppS"
                     /locus_tag="PKO10_05340"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012856247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HNW66602.1"
                     /translation="MTIETSEILKNAGTKISKAQKIIWNNLSDEEKDKKRKHCAKIGV
                     KYLTLYAFSTENWTRPKLEVEYLMKLLEKYLKNELETFMQNSIRFKAIGDLSKFSKSL
                     QKTIKDIEEKTSKNLRLTQVLALNYGSKDEIIRAIKKLNEKNLEINEKNFESCLDTAG
                     FGDVDLLIRTSGEIRLSNYLLWQNAYAEMFFTQTLWPDFSVKELDDIIENFNKRQRRF
                     GGV"
     gene            676..1182
                     /locus_tag="PKO10_05345"
     CDS             676..1182
                     /locus_tag="PKO10_05345"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HNW66603.1"
                     /translation="MEFSIFQNVLFTLFLINLVVLSLYDFKYKAVPDYLLLFAFFSSF
                     FITKFDIFEALQSAFIISGAFVILNFLVTFYIQNIKSRFLKDESLKTQTALGEGDIPL
                     IASFGVILGVYNTFIAIFLSAIVAMFYAIYLKNRKNEIEIPFIPFLVFGFLLEYFFNL
                     SNIFKDFY"
     gene            1179..>1618
                     /locus_tag="PKO10_05350"
     CDS             1179..>1618
                     /locus_tag="PKO10_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013136705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNW66604.1"
                     /translation="MRLSNYLHSQLAISFFPIFLGLFFITSVVFLVKIASLTSVITMT
                     SFELLKLYIYSIPQVIFYTLPISFFISLTICLSKLSSEYEMVVITSYGLNPLNIIKIF
                     LPLTTILTIFLLVISLGLIPKTKYLTKTFSEIKKKEANFNIKES"
BASE COUNT          598 a          178 c          232 g          610 t
ORIGIN      
        1 aaaagtcttg gtgtaatggg ttgacaatag aaacgtctga gatattaaaa aatgccggaa
       61 caaaaatatc aaaagctcaa aagataatat ggaacaacct atcagatgaa gaaaaagata
      121 aaaaaagaaa acattgtgca aaaatagggg ttaaatacct tactttatat gctttttcta
      181 ctgaaaactg gacaagacca aaacttgaag ttgaatatct tatgaaactt ttagaaaaat
      241 atttaaaaaa tgagctggaa acatttatgc aaaatagtat taggtttaaa gctattggtg
      301 atttaagtaa gttttcaaag agtttgcaaa aaactataaa agatattgaa gaaaaaacat
      361 caaaaaactt aagacttact caagttttag cactgaatta tggttcaaaa gatgagataa
      421 ttagagctat aaaaaaatta aatgagaaaa atcttgaaat aaatgaaaaa aactttgaat
      481 catgtcttga tacagcagga tttggagatg ttgatttgct catacgaaca agtggagaga
      541 ttagactttc aaactattta ttgtggcaaa atgcatatgc cgagatgttt tttacacaaa
      601 cactctggcc agattttagt gttaaagagc tagatgatat aatagaaaat tttaataaaa
      661 ggcaaagaag atttggtgga gtttagtatt tttcaaaatg ttctctttac actatttttg
      721 ataaatttag tggttttatc actgtatgat tttaaatata aagctgttcc tgattatctt
      781 ctgttatttg catttttttc atcttttttt attacaaagt ttgatatttt tgaagcattg
      841 caaagtgcat ttataatttc tggagctttt gtaatattaa attttttggt tactttttat
      901 atacaaaata taaaatcaag atttttaaaa gatgagagtt taaagactca aacagcacta
      961 ggagagggag atattccttt gattgcatct tttggtgtta ttttaggagt ttataataca
     1021 tttattgcaa tatttttaag tgcaattgta gctatgtttt atgcaatcta tttaaaaaat
     1081 agaaaaaatg aaattgaaat accttttatt ccatttctag tttttggttt tttattggag
     1141 tactttttta atttatcaaa tatttttaag gatttttatt gagattaagt aactatttac
     1201 actcacaatt agctattagc ttttttccta tatttttagg actttttttt ataacctctg
     1261 ttgttttttt ggttaaaatt gcaagcctta catctgtgat aacaatgaca tcttttgagc
     1321 ttttaaagct ttatatctat tcaattccac aagttatatt ttatacatta ccaatatctt
     1381 tttttatatc attaacaatt tgtttgtcaa agttatcaag tgaatatgag atggttgtta
     1441 ttacatctta tggtttaaac ccactaaata taattaaaat tttcttgcct ttaacaacta
     1501 tattaactat ttttttactc gtaatatctt taggtcttat tcctaaaaca aaatatttaa
     1561 caaaaacttt ctctgaaatt aagaaaaaag aggcaaattt taatataaaa gagagtga
//
LOCUS       DAOQAN010000313         1613 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_118248_length_1613_cov_2.853659, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000313 DAOQAN010000000
VERSION     DAOQAN010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118248_length_1613_cov_2.853659"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(135..701)
                     /locus_tag="PKO10_05355"
     CDS             complement(135..701)
                     /locus_tag="PKO10_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013135941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNW66605.1"
                     /translation="MSNRTLMRFTGVGGQGVLLAGEIFAAAKISNGGFGLKTATYTSQ
                     VRGGPTVVDITLQDEEIIYPYANDGEIDFMLSVAQISFDLFKNGVKDGATIVIEPNLV
                     HPSEEDKKRWNIIEIPIITIAKDEVGNVITQSILALAIANYFTGETIPKEVLRSTMLS
                     KIPPKLHELNNKAYDLGYKYAKEADNRK"
     gene            complement(711..1544)
                     /locus_tag="PKO10_05360"
     CDS             complement(711..1544)
                     /locus_tag="PKO10_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HNW66606.1"
                     /translation="MAFNYDEYLRTDKMPTLWCWGCGDGVILKALIRAIDKLGWNMDD
                     VCVVSGIGCSGRFSSYINCNTVHTTHGRTLPYATGIKMANPNKKVIVVGGDGDGLAIG
                     GNHTIHAARRNIDLTYILINNFIYGLTNSQTSPTTPKGMWTATMERGNIDPTFDSCKL
                     VEAAGASFVARETMIDPKKLERTLVKALEHKGFSYLEVFSNCHVNLGRKNKMASATSN
                     LEWIDSISLAKTKFDMLEESEKIGKYPTGVLKQDENALEYCEAYEKVKEAHKNKTMVE
                     L"
BASE COUNT          495 a          298 c          205 g          615 t
ORIGIN      
        1 aaagatgttt agaaaatata gattttagta agataaaatc tactgaagat ttgatagctt
       61 ttagtaaatc caataatatt ttaaaaattc ctaaagaaaa gaactaagaa gtaaaaactt
      121 cttagtttaa actcttattt tctattatca gcttcttttg catacttata acctaaatca
      181 taagctttgt tgttaagttc atgaagtttt ggaggaattt ttgagagcat tgtacttctt
      241 aaaacctctt taggaatagt ttctcctgtg aaatagtttg ctattgctaa tgctaaaata
      301 gattgagtaa taacatttcc tacttcatct tttgcaattg taataattgg aatttcaata
      361 atattccatc tttttttatc ttcttcacta ggatgtacca agtttggctc aataactatt
      421 gttgcaccat cttttacacc atttttgaat agatcaaaag atatttgagc aacagaaagc
      481 ataaaatcaa tctctccatc atttgcgtat ggatagataa tctcttcatc ttgaagtgta
      541 atatcaacaa cagttggccc accacgtacc tgtgaagtat aagtagctgt ttttaatcca
      601 aatccaccat tacttatttt tgcagctgca aaaatttcac ctgctagtaa aacaccttgt
      661 cccccaactc ctgtaaatct cattaaagtt ctatttgaca tattatttcc ttaaagctct
      721 accatagttt tatttttgtg tgcttctttt actttttcat aagcttcaca atattctagt
      781 gcattttcat cctgttttaa aactccagtt ggatatttac caattttttc actctcttct
      841 agcatatcaa attttgtttt tgctaaagat attgagtcaa tccactctaa atttgatgtt
      901 gcacttgcca ttttattttt tcttcctaaa tttacatggc aatttgagaa tacttctaaa
      961 tatgagaacc ctttatgctc aagagcttta actaaagttc tttcaagttt ttttggatca
     1021 atcatagttt ctcttgcaac aaaactcgca cccgctgctt ctacaagttt gcaagagtca
     1081 aaagttgggt ctatatttcc tctttccatc gtagctgtcc acattccttt tggagttgta
     1141 gggcttgttt gagagttcgt aagtccataa ataaagttat ttattaaaat ataagttaaa
     1201 tcaatatttc ttctagcagc atgaattgta tgatttccac caattgcaag accatctcca
     1261 tctccaccta caactataac ttttttattt ggattcgcca tttttattcc agttgcataa
     1321 ggaagtgttc ttccatgagt tgtatgaaca gtattacagt ttatgtatga gctaaatctt
     1381 ccagagcaac caattcctga aactacacaa acatcatcca tattccaacc aagtttatca
     1441 attgctctta ttaaagcttt taatataact ccatcaccac atccccaaca ccaaagtgtt
     1501 ggcattttat ctgttcttaa atattcatcg tagttaaaag ccatattaca ttccttttac
     1561 tttttctatt atttcaagtg gagaaagtgg tcttccattt gcttttaaaa gag
//
LOCUS       DAOQAN010000314         1611 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_118465_length_1611_cov_3.151671, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000314 DAOQAN010000000
VERSION     DAOQAN010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118465_length_1611_cov_3.151671"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..935
                     /locus_tag="PKO10_05365"
     CDS             <1..935
                     /locus_tag="PKO10_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNW66607.1"
                     /translation="EAISRYNISKNTILETLSIGVGAEQLTLFLDNLERYPVSLRYES
                     KEREDIRALENLQIKTNLGFKPLKTFASLSYEESPSIIQSEKALKVAYVYISPKSDFS
                     IQQYKDDANKLLKDLKLPDGYYFEWSGQSEFLQQAMSKLSFIIPIVLMFIFILIYLAL
                     KNLKYTLIIFCTLPFALSGGVFYIEYLGFNISIAVIVGFMALLGVAAETSIVMMLYLD
                     EAYKNIKEKRDYAVKEAIYHGAVLRLRPKLMTLFAILGGLIPIMYINSIGSEVMQRVA
                     APMIGGMLSSTILTLIIIPAIFYLVILKKDKIID"
     gene            995..1558
                     /locus_tag="PKO10_05370"
     CDS             995..1558
                     /locus_tag="PKO10_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNW66608.1"
                     /translation="MKILSKVASAMILSTGLLMASSYSVDNAHSNVGFTVKHMMVTNV
                     HGKFTSYDAKVEFDDATKTFKKLVANIDTNSIDTGIEKRDNHLRSDDFFAVEKFPKMT
                     FEMTSYKADGDDGKMTGNLTIRGITKPVVLEVEDIAILGDKVGFSLEGKINRQDFDLK
                     WNKAIELGGVAVSDEVKIKVDIEAKKQ"
BASE COUNT          619 a          168 c          271 g          553 t
ORIGIN      
        1 atgaagcaat aagtagatat aacataagta aaaacactat tttagaaact ttaagtatag
       61 gagttggtgc tgagcaatta actctctttt tagataattt agagagatat ccagtaagct
      121 taaggtatga atcaaaagag agagaagata taagagcatt agaaaatcta caaataaaaa
      181 ctaatctagg atttaaacca cttaaaacat ttgcctcttt gtcttatgaa gagagtcctt
      241 caataattca atcggaaaaa gcacttaaag ttgcttatgt ttatattagc ccaaaaagtg
      301 atttttctat acaacaatat aaagatgatg caaataaact tttaaaagat ttaaaacttc
      361 cagatggtta ctattttgag tggtcaggac aaagtgagtt tttacaacaa gcaatgtcaa
      421 aattaagctt tattattcca atagttttaa tgtttatatt cattttgatt tatttagcac
      481 taaagaattt aaaatataca ttgataatat tttgtacact tccttttgct ttaagtggtg
      541 gagtatttta tatagaatat ttaggattta atatctctat tgctgttatt gttggattta
      601 tggcactttt aggagttgct gctgaaactt ctatagttat gatgttatat ttagatgaag
      661 catataaaaa tattaaagaa aaaagagatt atgcagtaaa agaggcgata tatcatggag
      721 ctgttttaag acttcgacca aagctgatga cactttttgc aatacttgga ggacttatcc
      781 ctattatgta tataaactct attggaagtg aagttatgca aagagttgct gctcctatga
      841 ttggtggaat gttgagttca actatattaa ctttaattat aattcctgct attttttatt
      901 tagtaatatt aaaaaaagat aaaataattg attaataatt gattaattat agaaatatag
      961 aattaaactg ttaataaaaa ataaaggaga tagaatgaaa atattatcaa aagttgcgag
     1021 tgctatgatt ttatcaacag gtttactaat ggcttcttca tatagtgtag ataatgctca
     1081 ctcaaatgta ggatttacag taaaacatat gatggttaca aatgttcatg gtaaatttac
     1141 atcttatgat gcaaaagttg aatttgatga tgctactaaa acatttaaaa aattagttgc
     1201 aaatattgat actaattcaa ttgatacagg aattgaaaaa agagataatc acttaagaag
     1261 tgatgatttt tttgctgttg aaaagtttcc taaaatgaca tttgagatga catcttataa
     1321 agcagatggt gatgatggaa aaatgacagg aaatttaact attagaggaa ttacaaaacc
     1381 agtagtttta gaagttgaag atatagctat tttgggagat aaagttggtt tttctcttga
     1441 agggaaaatt aatcgtcaag attttgatct aaaatggaac aaagctatag aactaggtgg
     1501 tgtagcagtt tcagacgaag taaaaataaa agttgatatt gaagctaaaa aacaataaat
     1561 aaatacaaag taggattaac cctactttgt attttaatct cttggatagt t
//
LOCUS       DAOQAN010000315         1611 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_118476_length_1611_cov_2.820051, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000315 DAOQAN010000000
VERSION     DAOQAN010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118476_length_1611_cov_2.820051"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /locus_tag="PKO10_05375"
     CDS             complement(<1..777)
                     /locus_tag="PKO10_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HNW66609.1"
                     /translation="MLKNIFTKIENKIFNFSQDEKNIIEDYIKDEIIVKNGEDFEINS
                     KYKIGVLKIEKNFAILEDLVNPIKNIKLELDALNGAFNNDLILAKRVFNPRSKIKAKV
                     IKVLEGKKAEILVYVEDGSFFTLKENIELPSKNTKTYENGDVLIIDNKEFKEIKFIGN
                     LNDAKIDEFISLYIYEELYRDEEKIEIDEFVEDESKKETRVDLRDLPFCTIDPNSAKD
                     HDDAIFFDKENSTLFVAIADVSHFVQEGSKLDILAFKKSST"
     gene            885..>1611
                     /gene="ilvC"
                     /locus_tag="PKO10_05380"
     CDS             885..>1611
                     /gene="ilvC"
                     /locus_tag="PKO10_05380"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HNW66610.1"
                     /translation="MAINVFYDKDCNIELIKSKKVAMIGFGSQGHAHAENLRDSGVEV
                     VVGLRKDGSSWKKAEAKGFKVLTVAEATKIADVVMILLPDESQSEIYEKEIKPNLKDG
                     AYLAFGHGFNIHYKRIIPCKKMNVMMVAPKAPGHTVRSEFTKGGGIPDLIAVHQDASG
                     DTKQVALAYASAIGGGRTGIIETTFKDETETDLFGEQAVLCGGATALVQAGFEVLTEA
                     GYEPEMAYFECLHELKLIVDLMYE"
BASE COUNT          541 a          226 c          228 g          616 t
ORIGIN      
        1 tgttgaagat tttttaaaag ccaaaatatc aagtttgctt ccctcttgta caaaatgaga
       61 aacatctgca attgctacaa aaagagtgct attttctttg tcaaagaaaa tagcatcatc
      121 atggtctttt gcactatttg gatcaatcgt acaaaatggt aaatctctta gatcaactct
      181 tgtctctttt ttagactcat cttcaacaaa ctcatctatt tcaattttct cttcatccct
      241 ataaagctct tcataaatat acaaacttat aaattcatct atttttgcat cattcaaatt
      301 tccaataaac ttaatctctt taaactcttt gttatctatg attaaaacat ctccattttc
      361 ataagttttt gtatttttac taggtaattc tatattctct tttaaggtaa aaaaagaacc
      421 atcttcaaca tatactaaga tttcggcttt ttttccttca agtactttta taacttttgc
      481 tttaattttg cttcttggat taaaaactct ttttgctaaa attaaatcat tattaaatgc
      541 tccatttaat gcatctaatt ctaatttaat attttttatt gggtttacta aatcttctaa
      601 aatagcaaaa ttcttctcta tttttaaaac tccgatttta tattttgaat ttatctcaaa
      661 atcttcacca ttttttacta ttatctcatc ttttatataa tcttctatta tatttttttc
      721 atcttgcgaa aaattaaata tcttgttttc aatttttgta aatatatttt ttagcaattc
      781 ttatcctttt atttgcacta ttatatagtt tttcttttaa atctaatatt tatctatatt
      841 tttgtataat cttgctaaat ttttaaaaaa tagagggtag ataaatggca ataaatgtat
      901 tctatgacaa agattgtaat atagagttaa taaaatcaaa aaaagttgca atgattggat
      961 ttggttcaca aggacatgca cacgctgaaa atttaagaga tagcggtgta gaagttgttg
     1021 ttggattaag aaaagatggt agttcttgga aaaaagctga agctaaaggt ttcaaagttt
     1081 taacagttgc tgaagctaca aaaattgctg atgttgttat gatactttta ccagatgaga
     1141 gtcaatctga aatttatgaa aaagagatta aaccaaatct aaaagatggt gcttatttag
     1201 catttggaca tggatttaat attcactata aaagaattat tccttgtaaa aaaatgaatg
     1261 ttatgatggt tgctccaaaa gctccaggtc atacagttag aagcgaattt acaaaaggtg
     1321 gaggaattcc agatttaatt gcagttcacc aagatgcatc tggtgataca aaacaagttg
     1381 ctcttgctta tgctagcgca attggtggag gaagaactgg aattattgaa actactttca
     1441 aagatgagac tgaaactgac ctatttggtg agcaagctgt tctttgtgga ggagcaactg
     1501 ctttagttca agctggattt gaagtattaa cagaagctgg ttatgaacca gaaatggcat
     1561 actttgagtg tttacatgag ttaaaactaa ttgttgattt aatgtatgaa g
//
LOCUS       DAOQAN010000316         1608 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_118801_length_1608_cov_2.943979, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000316 DAOQAN010000000
VERSION     DAOQAN010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118801_length_1608_cov_2.943979"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1162
                     /locus_tag="PKO10_05385"
     CDS             <1..1162
                     /locus_tag="PKO10_05385"
                     /inference="COORDINATES: protein motif:HMM:NF018168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar basal body rod C-terminal
                     domain-containing protein"
                     /protein_id="HNW66611.1"
                     /translation="HIRQNADGSSTVFDSLKYNSDFTAKALYDADDTITYKLNNEFSV
                     SVKIGETITGNWDGDPNTPDTTMTVDNDNLTRAFMVKINSDPNMSKLVTAHNGEYTLD
                     ASGNKIPMYPNSDNYLKIESNLPGVDNEFTGRFSIERKTGTNVDGRETIYKNDKESKI
                     GQTDTVLTILDQDLNLKSGTMRAQVENLSTSNPNNKFQKYLDQLDQFAQTLADVNSSY
                     IRVGENDYVYGRDGSEAHNTAPFPPNGGDIVNMNLFSGSSIKTLKFDKNAVNDLNQQN
                     LDYLATLQWKDNLSFKGGAQNSNDVNSTSFVEFYRNLKVGVSTDKEEANYGFEVQDAI
                     ANNLGTAYNEVVKVNSDDEMIDLMKFQAAFSANAQVISAVDEMIQTLLGMKR"
     gene            1186..>1608
                     /locus_tag="PKO10_05390"
     CDS             1186..>1608
                     /locus_tag="PKO10_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar cap protein FliD N-terminal
                     domain-containing protein"
                     /protein_id="HNW66612.1"
                     /translation="MAEGILGLGQGQAASLNSDMLEKLKAVDRKATVEPIEKKLEKFE
                     SEKKVISDVTTKVNELFDAVKVFSLNQSTGTNAFQQKSANVSGDGVVFDSDDLSALKT
                     GSLRVEVKELAQKDVWQSRQFDGTTVTKDSKINMGNLTI"
BASE COUNT          635 a          180 c          306 g          487 t
ORIGIN      
        1 tcatattaga caaaatgctg acggttctag tacagttttt gattctttaa aatataattc
       61 agattttaca gcaaaagcac tttatgatgc tgatgatact ataacttata agttaaataa
      121 tgaattttct gtgagtgtta agattggtga gactataact ggaaattggg atggtgatcc
      181 aaatactcct gatacaacta tgactgttga taatgataat ttaacaagag catttatggt
      241 taaaataaat agtgatccaa atatgagtaa actagttact gctcacaatg gagaatatac
      301 tcttgatgca agtggtaaca aaattcctat gtatccaaat agtgataact atttaaaaat
      361 tgagtcaaat ttaccaggag ttgataacga gttcactgga agatttagta ttgaaagaaa
      421 aactggtacg aatgtagatg gaagagaaac tatatataaa aatgataaag agagtaaaat
      481 tggacaaact gatacagttt taactatttt ggatcaagat ttaaatctga aaagtggaac
      541 tatgagagct caagttgaga atttatctac atcaaatcca aacaataagt ttcaaaaata
      601 tttagatcag ttagatcaat ttgctcaaac tttagcagat gtaaatagct catacataag
      661 agttggtgaa aatgattatg tttatggaag agatggatca gaagcacata atacagcacc
      721 atttccacca aatggtggag atattgtaaa tatgaatctg tttagtggtt ctagtattaa
      781 aacattaaaa tttgataaaa atgcagtaaa tgatttgaat caacaaaact tggattattt
      841 agcaactctt cagtggaaag ataacttatc atttaaaggt ggagcccaaa attcaaacga
      901 tgtaaactct acttcttttg tagagtttta taggaactta aaagtaggag tttctactga
      961 taaagaagag gcaaactatg ggtttgaagt acaagatgca atagctaata atttgggaac
     1021 agcttataat gaagttgtaa aagtaaattc agatgatgag atgatagatt tgatgaaatt
     1081 tcaagcagca tttagtgcaa atgctcaagt gatttcagca gttgatgaaa tgatacaaac
     1141 tcttcttggg atgaagagat aaaacttact aataaggagt agcaaatggc agagggaatt
     1201 ttaggactag gacaaggtca agcagcttca ctaaatagtg atatgttaga aaaattaaaa
     1261 gctgttgata gaaaagctac agttgaacca atagaaaaaa aacttgaaaa atttgaaagt
     1321 gaaaagaaag ttatttcaga tgtaacaaca aaagtaaatg aactttttga tgctgttaaa
     1381 gttttctctt taaatcaaag tactggtaca aatgcttttc aacaaaaaag tgcaaatgtt
     1441 tctggagatg gagttgtttt tgattcagat gatttaagtg ctttaaaaac tggaagcttg
     1501 agagttgaag taaaggaatt agcacaaaaa gatgtttggc aatcaaggca atttgatgga
     1561 acaactgtta caaaagatag taagattaat atgggaaatt taactatc
//
LOCUS       DAOQAN010000317         1603 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_119370_length_1603_cov_2.616925, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000317 DAOQAN010000000
VERSION     DAOQAN010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119370_length_1603_cov_2.616925"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..889)
                     /locus_tag="PKO10_05395"
     CDS             complement(<1..889)
                     /locus_tag="PKO10_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013134177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HNW66613.1"
                     /translation="MKKLVLITIFLGIELLANNISKEDLGKALFFDVNLSKNRTQSCA
                     TCHNPEAAFIDDRDNGVSKMASLGDDLKSLGDRQAPTASYAKFSPDFHFNAKKGFYVG
                     GQFWDGREATLEGQAGGPPLNPIEMGIKSKKEVVDRLKENSFYVESFKSLFGKDIFKN
                     DENAYIAMTQAIASFERRDEFSPFDSKYDRYLKGEYDLTPLEDLGMSIFFSNNNNSCA
                     SCHVLKGEGKAGETFTNYEFHNIGVPVNNELREKNSPSYITDQLHELAIEKGGNAVIE
                     SIRTPGEIESLRKKGDFFLL"
     gene            complement(834..>1603)
                     /locus_tag="PKO10_05400"
     CDS             complement(834..>1603)
                     /locus_tag="PKO10_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014474948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNW66614.1"
                     /translation="NLVNIFLLVPFILSAKNVALFTNKFLYQNFGFIEFNSFSYFQIT
                     IFYTIAIFVFSDFTRYWLHRFLHTIPFLWEFHKVHHSAKVLTPITFYRVHFVENFLFG
                     LRYALSVGFVTGIFIYLFGAKINLYMIFGVNALVFISSIFGSNLRHSHVPFSYFSFIE
                     KWFLSPKQHQIHHDKKHFNKNYGGYIAIWDRVFGTLALSKDVNVLKFGLRKNQMSEYI
                     SIKDLYFRPFINLLKKGGFYEKISFNNNIFRNRVTSK"
BASE COUNT          587 a          259 c          179 g          578 t
ORIGIN      
        1 ccaataagaa gaaatcacct tttttacgta aagattcaat ttctccagga gttctgatac
       61 tttcaattac agcatttcca cctttttcaa tagctagttc gtgaagttga tcggtaatgt
      121 atgaaggtga atttttctct cttaattcat tatttactgg aacaccaata ttatgaaatt
      181 cgtagtttgt aaatgtttca ccagcttttc cttctccttt taaaacatga catgaggcac
      241 aagagttatt gttgtttgag aagaatattg acattcctaa atcttcaaga ggagttaaat
      301 catactctcc ttttaaatat ctatcatact ttgaatcaaa tggtgaaaat tcatcacgtc
      361 tttcaaaact agcaatcgct tgtgtcattg ctatataagc attttcatca tttttaaaaa
      421 tatcttttcc aaatagagat ttaaaactct ctacataaaa actattctct tttaatctat
      481 caactacctc ttttttactc tttattccca tttcaatagg atttagtggt ggaccacctg
      541 cttgtccttc caaagttgcc tctcttccat cccaaaattg accacctaca taaaatcctt
      601 tttttgcatt aaaatgaaaa tctggagaaa attttgcata agaagctgtt ggagcttgac
      661 gatcacctaa agattttaaa tcatcaccta aagatgccat ttttgaaaca ccattatccc
      721 tatcatctat aaatgctgct tctggattgt gacaagttgc acaactttga gttctatttt
      781 ttgataaatt aacatcaaaa aaaagtgctt tacctaaatc ctcttttgat atattatttg
      841 ctagtaactc tattcctaaa aatattgtta ttaaaactaa ttttttcata aaatcctcct
      901 ttttttaata aatttataaa tggtctaaaa tataaatctt ttatgcttat atattcactc
      961 atctgatttt ttctaagtcc gaattttagg acatttacat cttttgaaag agctagcgtt
     1021 ccaaaaactc tatcccaaat tgcaatataa ccaccataat ttttattaaa atgcttttta
     1081 tcatgatgta tttgatgttg ttttggagat aaaaaccatt tttctataaa tgaaaaatat
     1141 gaaaatggaa catgtgagtg tcttaaattt gaaccaaaaa ttgaagatat aaatactaaa
     1201 gcatttactc caaaaatcat atataaattt atttttgcac caaataaata tataaaaatt
     1261 cctgttacaa aaccaacact tagtgcatat ctaagcccaa aaagaaaatt ttcaacaaaa
     1321 tgaactctat aaaatgtaat aggtgttaaa acttttgcac tatgatgaac tttatgaaac
     1381 tcccataaaa atggaatggt atgtaaaaat ctatgtaacc aatatctagt aaaatcacta
     1441 aaaacaaaaa tagctattgt ataaaatatt gtaatttgaa aatatgaaaa agagttaaac
     1501 tctataaatc caaaattttg atataaaaat ttatttgtaa aaagtgccac attttttgca
     1561 cttaaaataa aaggaacaag caaaaaaata tttactaaat ttg
//
LOCUS       DAOQAN010000318         1600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_119770_length_1600_cov_1.726861, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000318 DAOQAN010000000
VERSION     DAOQAN010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119770_length_1600_cov_1.726861"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..595
                     /locus_tag="PKO10_05405"
     CDS             98..595
                     /locus_tag="PKO10_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66615.1"
                     /translation="MKKILPILTVVLIIAIIVIIFISMAKEQKMVVIKDGNVSQTPLE
                     IILGKYQDSDCGMIINDLTFASQVILQDGKTWFFHDHGGMANWLNNKDFKNSVKIFVM
                     TKDTNRYIDGKTAFYSTNEITPMRYGFGAYENNQDGFIDFEEMSLRVLRKQTLANPQH
                     KEHKH"
     gene            595..1305
                     /locus_tag="PKO10_05410"
     CDS             595..1305
                     /locus_tag="PKO10_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNW66616.1"
                     /translation="METFSLISIISIALLGSFGHCIGMCGGIVVAYSSTKIKSEYSKT
                     IQSVAHLLYSFGRVTTYTILGAIFGFIGGVVTFSNTTSGVFLLITGFLMVIVGFSLLG
                     KIKFLTILEHSCSKSPLYQKTFKNLLTSNSLFSFYLLGMLNGLLPCGFVYAFAITAAS
                     TGSAFWGAVVMFIFGLSTIPALFFLGFFVGFFKQSNLRDIFIKIASILVIIFGIYIAY
                     KGYEYIIDPTKSILNCHI"
BASE COUNT          578 a          176 c          246 g          600 t
ORIGIN      
        1 tatataatat aggctttgca gtttttccct ctgcaaagct tttttttatg ccaaaaaaga
       61 tacaatcacc cctaaattta ttatattttg gagacatatg aagaaaattt taccaatatt
      121 aacagttgtt ttaataatcg caataattgt aataatattt atatctatgg caaaagagca
      181 aaaaatggta gtaataaaag atggtaatgt ttctcaaact ccacttgaaa taattttagg
      241 taagtatcaa gatagtgatt gtggaatgat tattaatgat ttaacttttg cttctcaagt
      301 aattttacaa gatggaaaaa cttggttttt tcatgatcat ggtggaatgg caaattggtt
      361 aaataataaa gattttaaaa atagtgttaa aatttttgtt atgaccaaag atacaaatag
      421 atatatagat gggaaaactg ctttttatag tacaaatgaa ataactccaa tgagatatgg
      481 ttttggagct tatgaaaata atcaagatgg ttttatagat tttgaagaga tgagtttaag
      541 agttttaaga aaacagactt tagcaaatcc gcaacataaa gaacataagc attaatggaa
      601 acttttagcc ttatttcaat tatatcaata gctcttctag gctcttttgg gcattgtatt
      661 ggaatgtgtg gaggaatagt tgtagcgtat tctagtacaa aaataaaaag tgaatattca
      721 aaaactattc aaagtgttgc tcatctttta tactcttttg gaagagttac aacatataca
      781 attttaggag ctatttttgg ttttattggt ggtgttgtaa ctttttcaaa tactacaagt
      841 ggagtttttc tactaataac tggttttttg atggtaatag ttggattttc acttttagga
      901 aaaataaaat ttttaactat tttggagcat agttgttcaa aatcaccact ttatcaaaaa
      961 acattcaaaa atcttttaac atctaactct ttgtttagtt tctatctttt aggtatgtta
     1021 aatggtcttt taccttgtgg cttcgtgtat gcttttgcaa ttacagctgc aagtacagga
     1081 agtgcttttt ggggtgcagt tgttatgttt atttttggac ttagtactat tccagcacta
     1141 tttttcttag gattttttgt tggatttttt aaacaatcaa atttaagaga tatttttata
     1201 aaaatagctt ctattttagt aattattttt ggaatatata tagcgtacaa aggttatgaa
     1261 tatattattg acccaacaaa atcaatacta aattgtcata tataaaatta ttaggcttca
     1321 ttattgaagc tctttagaga tttgctactt taataatttt tttaaaatta tgtttaaaag
     1381 tagacaaata tgaaaggttc cctttttaaa ttggggaaca aaatttaaaa aggaggaaaa
     1441 aatgaaaaga tattttagct tttttggtat tttaatggca acatcaaatg caaaaatcaa
     1501 gatgttttgt gcaagatatg ataactatct tgaaaaatta ccaaatataa aaaatagttt
     1561 aataaaaact gaactaaaac caatcaaaca atattcaaca
//
LOCUS       DAOQAN010000319         1599 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_119882_length_1599_cov_1.805052, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000319 DAOQAN010000000
VERSION     DAOQAN010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119882_length_1599_cov_1.805052"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="PKO10_05415"
     CDS             complement(<1..261)
                     /locus_tag="PKO10_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HNW66617.1"
                     /translation="MLNNYEEIIEELKKIVKQKGLKYTEQREIVLSILLHANGHLTAE
                     EVYNQIKKDYPNSNVGIATVYRALSFLEEVELIASLNFGVDGK"
     gene            405..623
                     /locus_tag="PKO10_05420"
     CDS             405..623
                     /locus_tag="PKO10_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA domain-containing protein"
                     /protein_id="HNW66618.1"
                     /translation="MSLNDLDLQKKANIKAINCDDVLKKRLYSFGIIVGTEICVTAKS
                     LAKKTIEIKINQSKVALRHSEAINIKVG"
     gene            630..>1599
                     /locus_tag="PKO10_05425"
     CDS             630..>1599
                     /locus_tag="PKO10_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008244958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HNW66619.1"
                     /translation="MNKTIKIALVGQPNVGKSMLINSISNSRLKVGNFSGVTVEKKDV
                     IFKYKDYNIKIIDLPGSYSLENYSLEEKVVKNFLNQNEYDIILNVIDSTNLQRNLLLT
                     SELLDLNKKMVIALNMSDEAKKENILINNEKLSSLLNTPCIKTSATKKDGITELLEQI
                     IKTFKEDKTTFKHSFTTHILNHDEILAKRFNFIKNLIQQCVKIEEIKEKTTTEKIDSI
                     LMNKFIGLPIFLFLMWGLFQLTFTLGEIPMDYIDTFFSSFIDVTKNIIGDNQLSSLLG
                     DGVIAGVSAVVMFLPNILILFLGISLLEGTGYMSRVAFLLDGTFHKF"
BASE COUNT          626 a          196 c          196 g          581 t
ORIGIN      
        1 ctttccatct acaccaaaat taagtgaagc aataagctca acttcctcta aaaaacttaa
       61 agctctataa acagttgcaa ttccaacatt tgagtttgga taatcttttt ttatttgatt
      121 gtaaacctct tctgcagtta aatgaccatt cgcatgaagt aatatactta aaacaatctc
      181 tctttgctca gtatatttaa gacctttttg cttaacaatc ttttttaact cttctataat
      241 ctcttcataa ttattcaaca taaaaacacc tttaaaaaat aaaaatctag tatatctaaa
      301 gtttaaaaaa aattcaaata tttcttatat tttattgata tatgttaata caatttaatc
      361 tattttacat tataataatc attattaata tttaaaggta taaaatgtct ctaaatgact
      421 tagatttaca aaaaaaggct aatataaaag ctataaattg tgatgatgtt ttaaaaaaaa
      481 gactctattc ttttgggata attgttggaa ctgaaatttg tgtaacagcg aaatctttag
      541 ctaaaaaaac tattgagata aaaataaatc aatcaaaagt tgcattaaga catagcgaag
      601 ctatcaatat taaagttgga taaaataaaa tgaataaaac tataaaaata gctcttgtag
      661 gtcaacctaa tgttggtaag tcaatgctta ttaactcaat ttcaaattca agattgaagg
      721 taggtaattt ttcaggtgtt actgttgaaa aaaaagatgt aatttttaaa tataaagatt
      781 ataatattaa aattatcgat ttacctggtt catattcact tgaaaactac tcattagagg
      841 aaaaagttgt aaaaaacttt ttaaatcaaa atgagtatga tattatctta aatgttattg
      901 attcaacaaa tcttcaaaga aatcttctac ttaccagtga attattagat ttaaataaaa
      961 aaatggttat tgcactaaat atgagtgatg aagcaaaaaa agagaatatt ttaattaata
     1021 atgaaaaact atcatctctt ttaaatacac cttgtataaa aacaagtgct acgaaaaaag
     1081 atggaattac agagcttcta gaacaaatta taaaaacttt caaagaagat aaaactactt
     1141 ttaaacactc ttttaccaca catattttaa accatgatga gattttggct aaaaggttta
     1201 attttataaa aaaccttatt caacaatgcg taaaaattga agagattaaa gaaaaaacta
     1261 ctactgaaaa aattgactca attttgatga ataaatttat aggtcttcct atatttttat
     1321 ttttaatgtg gggattattt cagctaactt ttactttagg agaaattcct atggactata
     1381 ttgacacttt tttttcttca tttatagatg ttactaaaaa tataattgga gataatcaac
     1441 tctcttctct tttaggagat ggtgtaatag ctggagttag tgctgttgtt atgtttttac
     1501 caaatatttt gattttattt ttaggaattt ccctacttga aggaactggc tatatgagta
     1561 gagttgcttt tttactagat ggaacttttc ataaatttg
//
LOCUS       DAOQAN010000320         1598 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_119992_length_1598_cov_2.173040, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000320 DAOQAN010000000
VERSION     DAOQAN010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119992_length_1598_cov_2.173040"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1167)
                     /locus_tag="PKO10_05430"
     CDS             complement(<1..1167)
                     /locus_tag="PKO10_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNW66620.1"
                     /translation="MSYLSYNAKDVQIDASAETLLLEDDEDLKFFRESFKKYENSNFL
                     IVTFSPKKNLLDDETLNTIKNISNDFEKVENIAKVDSILTVPLLQSPIRPISDLVAGV
                     DSLSTKEFDKSLVENEFLNSPLYKNALVSADFKTTALILHLKDDKKYFEFIEKRENLL
                     KKQDVQALTKEDIEELERNNLEFKEYRETSRVKDSKNIEDIRNIIKNYEGDAKIFLGG
                     VSMIANDAVHFVKNDLLIYGISLIFIFIFVLYYIFRSFRWVFIVLFICFISILSTSGI
                     LGIFSWEVTVISSNFVALQLIITMSMVVHLVERYKELYFKYKNASQYKLTINTVLSKL
                     IPSFFAIITTVVGFSSLVLSNIEPVINLGLMMSVGILVSLVLTFIYFPIFLILIG"
     gene            complement(1243..>1598)
                     /locus_tag="PKO10_05435"
     CDS             complement(1243..>1598)
                     /locus_tag="PKO10_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014474639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNW66621.1"
                     /translation="KDAWNSIDEAKQKEFSEVFEDKIKKSYSDKLELYNDQKVKILTL
                     EPYNNTRLQLKTELIGKEGNYSINYNFYEKNGEWFIYDIDLVGVSIIQTYRQQFAGLL
                     KEKSFDEMFKQFKNQ"
BASE COUNT          579 a          233 c          173 g          613 t
ORIGIN      
        1 tccaataagt attaaaaaga ttggaaaata tataaaagtt aaaactaaag ataccaaaat
       61 accaacactc atcataagcc ctagatttat aacaggttca atatttgata aaactaaaga
      121 tgaaaatcca actactgttg taatgattgc aaaaaaagat ggaattagtt ttgataaaac
      181 agtgtttatt gttagtttat attgacttgc atttttgtat ttaaagtaca actctttata
      241 tctttcgact aagtgaacta ccattgacat tgttattata agttgtaagg caacaaaatt
      301 tgaagatatt actgtaactt cccaagaaaa tattcccaaa atcccagatg tacttaaaat
      361 tgagataaaa cagataaata gaactataaa aacccatcta aagcttctaa aaatgtagta
      421 taaaacaaag ataaaaataa aaatgagact aattccataa atcaacaaat catttttgac
      481 aaaatgaaca gcatcattag ctatcatact aactccgcct aagaatatct ttgcatcacc
      541 ttcataattt ttgataatat ttctaatatc ttcaatattt ttgctatctt ttactcttga
      601 agtttctcta tactctttaa actctaagtt atttctttct agttcttcta tatcttcttt
      661 agttaaagct tgaacatctt gtttttttaa aagattctct cttttttcaa taaattcaaa
      721 atatttttta tcatctttta ggtgaaggat taaagcagtt gttttaaaat ctgcacttac
      781 aagagcattt ttatataaag gtgagtttaa aaactcattt tctactaaag acttatcaaa
      841 ctcttttgta cttaaactat caactcctgc aactaagtct gaaatagggc gaattggaga
      901 ttgaagaagt ggaactgtaa gaattgaatc aacttttgca atattttcaa ctttttcaaa
      961 atcattagag atatttttta tagtatttaa agtttcatca tctaaaagat ttttttttgg
     1021 actaaaagta actattaaaa agtttgagtt ttcatatttc ttaaaactct ctctaaaaaa
     1081 ttttaaatct tcatcatctt caagaagcag tgtttctgca cttgcatcaa tttgaacatc
     1141 ttttgcatta taagaaaggt agctcacaaa aattgtggct actattaaaa ataaaaaagg
     1201 atacttaaaa attaaagtat catatatttt tttatacata aactattgat ttttaaactg
     1261 tttaaacatt tcatcaaaag atttctcttt tagaagtcca gcaaattgtt gtctataagt
     1321 ttgtataata ctaacaccta ctaaatcaat atcatagata aaccattcac catttttttc
     1381 ataaaaattg tagtttatac tataatttcc ctcttttcct ataagttcag tttttagttg
     1441 aagtcttgta ttgttatatg gttcaagagt taaaattttt actttttggt cattgtaaag
     1501 ttctagttta tcgctataag atttttttat cttatcttcg aatacttcac taaactcttt
     1561 ttgttttgcc tcatctatac tattccaagc atcttttc
//
LOCUS       DAOQAN010000321         1596 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_120194_length_1596_cov_2.530824, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000321 DAOQAN010000000
VERSION     DAOQAN010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120194_length_1596_cov_2.530824"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="PKO10_05440"
     CDS             <1..549
                     /locus_tag="PKO10_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPP family protein"
                     /protein_id="HNW66622.1"
                     /translation="IDKSNMFWAWIGSFLGIIAISYFHMDILDDKDLTLVVGSFGASA
                     VLIYGVPNSPLSQPRNLIGGHLLSAIVGVISYKLFSSNLFLATAIAVSTSILIMQLTL
                     TLHPPGGATSLIAVIGGEQIHELEFFYILIPVFSGALILFLIAFIVNNIPKNRAYPES
                     FKNYLKKKYQRYKRRSLKRDKR"
     gene            complement(552..1457)
                     /locus_tag="PKO10_05445"
     CDS             complement(552..1457)
                     /locus_tag="PKO10_05445"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HNW66623.1"
                     /translation="MDNPISLNSTKDSSLNKDKKNREQEEKEFESVKPEAVNIPIVSM
                     LPENIEILGLLPYDNQNIEKNEKNPETKIEYELKSEIDFGNQSSNDILSQEDEEELLQ
                     TSLNEFDEQIKKVSKNSQTFTKSYEKKVSKSNLPKEQRRNLNNYDELIIRVDSKTNIM
                     ELFAKNGENEEKIKSYIVSTGKDSIKKPLGVGRISQISLNPVWYPTADTKKSFAKKGI
                     ILPNVVPPNHKYNYMGMAKLNLTHSVDGNTTYRIHGTLNEKTLGSNESAGCIRMRNND
                     VVELAILLEEFANIKTLNKIKVVLI"
BASE COUNT          458 a          220 c          202 g          716 t
ORIGIN      
        1 attgataaat caaatatgtt ttgggcttgg ataggttctt ttttagggat tattgcaatt
       61 tcatattttc acatggatat tttagatgat aaagatttaa ctttagttgt tggttctttt
      121 ggagcaagtg ctgtattgat ttacggagtt cctaattctc ctttatctca gcctagaaat
      181 ttaataggag gacatttatt atctgctatt gtaggagtta tctcatataa gcttttctct
      241 tcaaatctat ttttagcaac agcgattgct gtttcaacat ctatattaat tatgcaatta
      301 actctaactt tacatcctcc aggaggtgca acttcattaa tagcagttat tggaggtgaa
      361 caaatacatg agcttgaatt tttttatatt ttaattccag tttttagtgg agcattaatt
      421 ctatttttaa ttgcatttat agtaaataat attcccaaaa atagggcata tccagagagt
      481 tttaaaaatt atttaaaaaa gaagtatcaa agatataaaa gaagaagttt gaaaagagac
      541 aaaaggtagt tttagattaa aactactttt attttattta aagttttaat attggcaaac
      601 tcttctagta aaattgctag ttctacaaca tcgttgtttc tcattctaat acatccagca
      661 ctttcgttgc ttcctagagt tttttcattt aaagttccat gaatcctata tgttgtattt
      721 ccatcaacac tatgagttag atttaatttt gccattccca tatagttgta tttatgattt
      781 ggtggtacaa catttggtaa aattatccct ttttttgcaa agcttttttt agtatcagca
      841 gttggatacc aaactggatt tagtgaaatt tgtgaaattc ttccaactcc taatggtttt
      901 tttatactat cttttcctgt tgaaacaatg tagcttttta ttttttcttc attttcacca
      961 ttttttgcaa aaagttccat aatatttgtt tttgaatcta ctcttattat taactcatcg
     1021 tagttattta gatttcttct ttgttctttt ggtaaatttg attttgaaac ctttttttca
     1081 taagattttg taaaagtttg actgtttttt gagacttttt ttatttgttc atcaaactca
     1141 tttaggcttg tttgtaaaag ttcttcttca tcctcttggc ttaaaatatc atttgaagat
     1201 tgatttccaa aatctatctc actttttagt tcatactcta ttttagtttc aggatttttt
     1261 tcattttttt ctatattttg attatcataa ggcaaaagtc ccaaaatttc aatattttca
     1321 ggcaacattg aaactattgg aatatttaca gcttcaggtt ttacactttc aaactctttt
     1381 tcttcttgtt ctctattttt tttatctttg tttaaagagc tatcttttgt actatttaaa
     1441 ctaattggat tatccatatc tataaaaagt tttcttttat caatttgatt tacaattttt
     1501 atatcaatct cttttatata aggtttttga tcttttactt atttagaagc attttgtatt
     1561 gtaacttttt atatctcttt ttcatcatat ttattt
//
LOCUS       DAOQAN010000322         1592 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_120634_length_1592_cov_3.588159, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000322 DAOQAN010000000
VERSION     DAOQAN010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120634_length_1592_cov_3.588159"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..171
                     /locus_tag="PKO10_05450"
     CDS             <1..171
                     /locus_tag="PKO10_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HNW66624.1"
                     /translation="VLIGTAISFMMAVVALSLPEAMILKRILSIKLIAIFFSIVGFSI
                     LIVGYLLNYLVG"
     gene            176..412
                     /locus_tag="PKO10_05455"
     CDS             176..412
                     /locus_tag="PKO10_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011138668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNW66625.1"
                     /translation="MKIEILGTGCTKCKALEEAVKKAVAQIGGFHEVKKVEDIVEIMN
                     YGVMSTPALVVDGIVKSSGKLLSIDEIINLLKGK"
     gene            412..723
                     /locus_tag="PKO10_05460"
     CDS             412..723
                     /locus_tag="PKO10_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNW66626.1"
                     /translation="MDNFLQTIGAINDETRVKILNFINIHGEVCVCDIENSFSMIQSR
                     VSRHLKILKDAGFLKVDRKGRWAYYSIRAPLDIFRESILKEISYLQLDIPILKKECDI
                     C"
     gene            717..>1592
                     /locus_tag="PKO10_05465"
     CDS             717..>1592
                     /locus_tag="PKO10_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HNW66627.1"
                     /translation="MLIASLIFIITLTFVIVQPKNIQIGTSAIFGAFIALIFGVVTFS
                     DVLDVTNIVWDATLAFIGIIILSLVLDEIGFFEWAALKMAKFSNGSGLKMFIYSILLG
                     AFVSALFANDGAALILTPILLAKMRILQLNMKTIVAFLLAGGFISDSASLPFVFSNLT
                     NIVTANYFSIGFAQYFFDMIIPFIVSVIASTIFLWLILRKDIPKKVDITLLKEPKSVI
                     KNMKLFYFSWVFLAFLLCAYFLGDAYDLPISIFALGGATIFLIIATISKSVEPLKIIK
                     EAPWQVVWFSIGLYIV"
BASE COUNT          535 a          154 c          281 g          622 t
ORIGIN      
        1 gttttaatag gaacagctat atcttttatg atggcagttg ttgctctaag tcttcctgaa
       61 gctatgattt taaaaagaat attatctata aaattaatag caatattttt tagtatcgtt
      121 ggtttttcaa tacttattgt tggatattta ttaaattatt tagtaggata aaaaaatgaa
      181 aatagagatt ttaggaacag gttgtacaaa atgtaaggct ttagaagaag ctgtaaaaaa
      241 agcagttgct caaattggtg gatttcacga agttaaaaaa gtagaagata tagttgagat
      301 tatgaactat ggtgttatga gtactccagc tttggttgtt gatggtattg ttaaaagtag
      361 tggaaaatta ttaagtattg atgaaattat taatttatta aaaggaaaat agtggataat
      421 tttttacaaa caataggtgc tataaatgat gaaactagag ttaagatact gaatttcata
      481 aatattcatg gtgaagtttg tgtttgtgat attgaaaact ctttttcaat gatacaatca
      541 agggtatcaa gacatcttaa aattttaaaa gatgcagggt ttttaaaagt agatagaaaa
      601 ggtcgatggg cttattatag cattagagca cctttggata tttttagaga atctatttta
      661 aaagagataa gttatttaca acttgacatt ccaattttaa aaaaagagtg tgatatatgc
      721 taattgctag tttaatattt attataactt taactttcgt gattgttcag ccaaaaaata
      781 ttcaaattgg aacaagtgct atttttggag cttttattgc acttatattt ggagttgtaa
      841 cttttagtga tgttttggat gttacaaata tagtttggga tgcaacttta gcatttattg
      901 gaattattat tttatcttta gttttggatg agattggttt ttttgagtgg gcagctttaa
      961 agatggcaaa gttttcaaat ggtagtggat taaaaatgtt tatctactct attttgcttg
     1021 gagcgttcgt ttcagctctt tttgcaaatg atggagcggc tttgatatta actccaattt
     1081 tattagcaaa aatgagaata ttgcaactaa atatgaaaac aatagttgct tttttactag
     1141 caggtggatt tataagtgat agtgcatcac taccttttgt attttcaaat cttacaaata
     1201 tagtaactgc aaactatttt agtataggtt ttgcacaata tttctttgat atgataattc
     1261 catttattgt aagtgttata gcttctacaa tttttttatg gttgatttta agaaaagata
     1321 ttccaaaaaa agttgatata actttattaa aagagccaaa aagtgtaata aaaaatatga
     1381 aactcttcta tttttcatgg gtatttttag catttctttt atgtgcttat tttttaggag
     1441 atgcttatga tttacctatt tcaatctttg cattaggtgg agcaactata tttttaatta
     1501 ttgcaactat ttcaaaatct gttgaacctc tgaaaattat aaaagaagcg ccttggcaag
     1561 ttgtgtggtt tagtattgga ctttatatag tt
//
LOCUS       DAOQAN010000323         1591 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_120783_length_1591_cov_2.391276, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000323 DAOQAN010000000
VERSION     DAOQAN010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120783_length_1591_cov_2.391276"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            119..1351
                     /locus_tag="PKO10_05470"
     CDS             119..1351
                     /locus_tag="PKO10_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNW66628.1"
                     /translation="MKKLLLVLLILSLFINHLNANEKDLNIIQKDKKEYRLLDKESIQ
                     TRQQSQKYDWISPIDLSGNIGRNHSFSDENSRNLNNKNTKSASIGFTQSIFESGGIGF
                     KMDYADNRFKYEMLSWENQNSQLIYTIYNTLLDIKKLKLEINQNDYRIVNKDIELIIK
                     KIQYEAGKTDIIELNNAIMSKNLIQKEEIALKNSLKEKEQELAKYTDLKYDEIEILDF
                     QETNKDIFLDKNYSILEADAKVGMLNSEYKIKKSQYLPKLSLSAKASYSNQDEKFNSM
                     INDTNKDDATSSGSLILSMPLYDYTKSSKIQESKIEVLKQKALVNDLKNETAFEYEQI
                     FTKIDTYLKQNETINKNIKLYEELIGANQISSEAGMTSKYDLEILENTKTINEFDILI
                     NKINILQQYAKLYFKIKN"
     gene            1365..>1591
                     /locus_tag="PKO10_05475"
     CDS             1365..>1591
                     /locus_tag="PKO10_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNW66629.1"
                     /translation="MSNLKEELNNYKDKKSSYKYIGLLIIVFVIAILIYIFFPKSDSQ
                     KVEYITSKVNSGDLKVVVSASGNIKPTNSVE"
BASE COUNT          713 a          125 c          229 g          524 t
ORIGIN      
        1 agaattttat tacaatattg ataataatta tcttaatttt gtaataaaat taatttttgt
       61 taaaatagta ttaaaatata tcatatataa ttttgcataa actttaaagg aaagagaatt
      121 gaaaaaacta ctattagttt tattaatttt gagtctattt ataaatcatt taaatgcaaa
      181 tgagaaagat ttaaatatta ttcaaaaaga taaaaaagag tatagacttt tggataaaga
      241 gtctatacaa acaagacaac aaagtcaaaa atatgattgg ataagtccta ttgatttaag
      301 tggaaatatt ggaagaaatc actctttttc tgatgaaaat agtcgtaatt taaataataa
      361 aaatacaaaa agtgcatcta ttggatttac tcaatctatt tttgaatcag gtggaatagg
      421 ttttaaaatg gactatgctg ataatagatt taaatatgag atgttgtctt gggagaatca
      481 aaattcacaa cttatttata caatttataa tactttatta gatattaaaa aattgaagtt
      541 agagataaat caaaatgatt atagaattgt aaataaagat attgagttaa taattaaaaa
      601 aattcaatat gaagctggaa aaactgatat tatagaactc aataatgcaa ttatgagtaa
      661 aaatttaata caaaaagagg agatagcttt aaaaaatagc ttaaaagaga aagagcaaga
      721 gttagcaaaa tatacagatt tgaagtatga tgagattgaa attttagatt ttcaagaaac
      781 taacaaagat atttttttgg ataaaaacta ctcaatttta gaagcagatg caaaagttgg
      841 gatgttaaat agtgaatata aaataaaaaa gagtcaatat cttccaaaat taagtttaag
      901 tgcaaaagct agttactcaa atcaagatga gaagtttaat agtatgatta atgatacaaa
      961 taaagatgat gcaacttcta gcggaagttt aattttatct atgcctttat atgattatac
     1021 aaaatcttca aaaattcaag agtcgaaaat agaggtttta aaacaaaaag ctttggtaaa
     1081 tgatttgaaa aatgaaacag catttgagta tgaacagatt tttacaaaaa ttgatacata
     1141 tttaaaacaa aatgagacaa taaataaaaa tataaaactt tacgaagagt taataggtgc
     1201 aaatcaaatt tcaagtgaag ctggaatgac atcaaagtat gatttagaga ttttagaaaa
     1261 tacaaaaact ataaatgagt ttgatatttt gataaacaag ataaatattt tgcaacaata
     1321 tgcaaaacta tattttaaga taaaaaatta aaggttaaaa aattatgagt aatttaaaag
     1381 aagagttaaa taattacaaa gataagaaaa gttcttacaa atatataggt ttattaatta
     1441 ttgtttttgt tattgctatt ttaatttata tctttttccc aaaaagtgat agccaaaaag
     1501 tagagtatat aacttcaaaa gtaaacagtg gtgatttaaa ggttgttgtt agtgctagtg
     1561 gaaatataaa acctactaat agtgttgaaa t
//
LOCUS       DAOQAN010000324         1585 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_121548_length_1585_cov_2.405882, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000324 DAOQAN010000000
VERSION     DAOQAN010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121548_length_1585_cov_2.405882"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /locus_tag="PKO10_05480"
     CDS             <1..819
                     /locus_tag="PKO10_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014472845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal helicase domain-containing protein"
                     /protein_id="HNW66630.1"
                     /translation="IIFCRTKKDVDRLSTFLLSQGFMAKSLHGDMEQKQREEAIKAFK
                     TSKLEILIATDVAARGLDVNDVTHVFNYHLPFDGESYVHRIGRTGRAGKDGMAISIVT
                     PHEFRTLQRIEKTIGAKLESKVVPNISTVKQKKIDELKQSILDQEIKDYAITIVEELK
                     EEFDISTIAFKLASIIASKTYVKGNNHIGKSESDISRLIENMLRNSDRGDDRRGSSRG
                     RGRSGGGQRRDSGSRDDRGGQRRPSTSRSRSSSSNSNKDSSDKPARSSRPRRRD"
     gene            1247..1465
                     /locus_tag="PKO10_05485"
     CDS             1247..1465
                     /locus_tag="PKO10_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HNW66631.1"
                     /translation="MADQNIGTVKWFNSEKGFGFIQIENGNDEFFIHHSEIQSSGYGR
                     VSLDEGQRVSFEIGRNEKGPQAKNVRSI"
BASE COUNT          667 a          177 c          254 g          487 t
ORIGIN      
        1 attatatttt gtagaacaaa aaaagatgtt gatagattat ctacattttt attatctcaa
       61 ggttttatgg ctaaatcatt acatggtgat atggaacaaa aacaaagaga agaagctata
      121 aaagcattta aaacatcaaa acttgaaata ctaattgcaa cagatgttgc agcaagagga
      181 cttgatgtaa atgatgtaac tcacgtattt aattatcatc taccatttga tggtgaatca
      241 tatgttcaca gaattggaag aactggaaga gcaggaaaag atggaatggc tatttcaatt
      301 gtaacacctc atgagtttag aactcttcaa agaattgaaa aaacaattgg tgcaaaatta
      361 gaatcaaaag ttgttccaaa tatttcaact gtgaaacaga aaaaaataga tgaacttaag
      421 caatctatct tagatcaaga gataaaagat tatgcaataa ctattgttga ggagttaaaa
      481 gaagagtttg atatttcaac aattgcattt aaactagctt caattattgc ttctaaaact
      541 tatgtaaaag gaaataatca cataggaaaa agcgaaagtg atataagcag actaatcgaa
      601 aatatgttaa gaaatagtga tagaggtgat gatagaagag gttcttcaag aggaagagga
      661 agaagtggtg gtggacaaag aagagattct ggttcaagag atgatagagg tggacaaaga
      721 agaccatcaa catcaagaag tagaagtagc tcatctaatt caaataaaga ttcatctgac
      781 aaaccagcaa gaagctcaag acctagaaga agagattaat tctaaaaagt tatttcttca
      841 tacttgtttt ctttcacttg ggatttttag aagtatgtaa ctttatgatt atatgaagaa
      901 aactagtaga aataaagata taaaaattta gagaaaatta ttcaatttca tagaatttac
      961 actaaaaaat agttaaattc tatgtatgaa agttttgtaa atatttattt aatcgtataa
     1021 tataaataaa tttaaaaata ttgtaaaata gcttgctttt atatttttat tcagttagaa
     1081 tactcgtctt gaagatgatt tagggttttt gactacatct tttgtttgct tttattgata
     1141 actacaagaa attgatacta acttacttaa tacaaattaa aactccactt taactttata
     1201 gattactttt taattaaagt agaaaaataa attaaaagga aatgccatgg cagatcaaaa
     1261 tattggaaca gtaaaatggt tcaactcaga aaaaggtttt ggatttattc aaatagaaaa
     1321 tggaaatgat gaatttttca ttcaccacag tgaaattcaa agttcaggat atggaagagt
     1381 atctttagat gaaggacaaa gagtatcttt cgaaattgga agaaatgaaa aaggtcctca
     1441 agcaaaaaac gttagatcaa tataactttt ttaaaatcag attttttcta ggctatctag
     1501 cttagaaaaa tctaattcac ctaaaactaa tataactaca acaaattata atcaactata
     1561 taaaatagat aaaaacaaaa aaatt
//
LOCUS       DAOQAN010000325         1576 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_122647_length_1576_cov_3.002630, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000325 DAOQAN010000000
VERSION     DAOQAN010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122647_length_1576_cov_3.002630"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="PKO10_05490"
     CDS             complement(<1..186)
                     /locus_tag="PKO10_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002851936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNW66632.1"
                     /translation="MIREIITYPNKLLRTKSKDVEEFNSELHTLLDDMYETMIAASGV
                     GLAAIQIAVPLNVLIINP"
     gene            complement(195..779)
                     /gene="clpP"
                     /locus_tag="PKO10_05495"
     CDS             complement(195..779)
                     /gene="clpP"
                     /locus_tag="PKO10_05495"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HNW66633.1"
                     /translation="MSYIPYVVEKTGRGERSYDIYSRLLKDRIIMLSGEINDAVASTV
                     VAQLLFLEAEDPEKDIYLYINSPGGVVTSGMSIFDTMNYIKPDVCTICIGQAASMGAF
                     LLSSGTKGKRYSLPNSRIMIHQPLGGARGQATDIQIQAKEIQRLKDTLNSILASQTGQ
                     DVKTIEKDTDRDNFMSAAEACKYGLIDEVIEKHK"
     gene            complement(794..>1576)
                     /gene="tig"
                     /locus_tag="PKO10_05500"
     CDS             complement(794..>1576)
                     /gene="tig"
                     /locus_tag="PKO10_05500"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HNW66634.1"
                     /translation="EGFVDGVAFEGGKAEKYPLQIGSGSFIPGFEEQVIGMKYEEQKD
                     ITVQFPKDYQAKDLAGKDAVFKVTLHEIQERKVGELNDEFAKQMLPNEKDATIDTLRE
                     KIKEQMVATEKATYYREELKPQFLDTLVEKINFALPKTVVEQEINFALNGKIRSMSEA
                     EINELKENPSKIEEIKEELKTDATNSVKATFIIDALAKAESIEVNDQEVTQLLYFEAL
                     QMGQNPQNVIKQYQEAGYLPAIKMSIIEEKVISKLFDEKLEK"
BASE COUNT          471 a          290 c          200 g          615 t
ORIGIN      
        1 aggatttata attaagacat ttagtggaac tgctatttga atagctgcta atcctacacc
       61 agaagctgct atcatagttt catacatatc atccaaaaga gtgtgaagtt cgctattaaa
      121 ctcctcaaca tcttttgatt ttgttcgaag taatttgttt ggatatgtaa taatctctct
      181 aatcatagtt cttcttattt atgtttttca ataacttcgt ctataagacc atatttacaa
      241 gcttcagctg cactcataaa gttatctcta tcagtatctt tttcaatagt ttttacgtct
      301 tgtccagttt gagatgctaa aatactattt aaagtatctt ttagtctttg aatctcttta
      361 gcttgaattt gaatatccgt tgcttgacct ctagcacctc ctaaaggttg atgaatcata
      421 attctagagt ttggaagaga gtatctcttt ccttttgtcc ctgaactcag taaaaaagca
      481 cccatagaag cagcttgtcc aatgcaaata gtacaaacat ctggcttgat ataattcata
      541 gtatcaaaaa tagacattcc agaagttaca actccacctg gagagtttat gtataagtag
      601 atatcttttt caggatcttc agcttctaaa aatagaagtt gagccaccac agttgaagca
      661 actgcatcgt tgatttcacc acttaacatg ataattctat cttttaaaag tcttgaataa
      721 atatcgtaac ttctttctcc tcgacctgtt ttttcaacta cgtatggtat ataactcatt
      781 ttctatccta aaattatttt tctaatttct catcaaaaag tttagagata actttctctt
      841 caataattga cattttaatt gcaggtaaat atccagcttc ttgatattgt ttaataacat
      901 tttgtggatt ttgacccatt tgaagtgctt caaaatatag aagttgagta acttcttggt
      961 cattaacttc aatactctca gcttttgcta aagcatcgat aataaacgta gcttttacag
     1021 agtttgtagc atcagttttt aactcttctt taatctcttc tattttagaa gggttctctt
     1081 ttaactcatt aatttcagct tcactcatag atcttatttt accatttaat gcaaagttaa
     1141 tctcttgctc aactacagtt ttaggtaaag caaaattgat tttttcaact aaagtatcta
     1201 aaaattgtgg ttttaactct tctctataat aagttgcttt ttcagttgct accatttgct
     1261 cttttatttt ttctcttaaa gtatcaatag ttgcatcttt ttcatttgga agcatttgtt
     1321 ttgcaaactc atcatttaac tcacctactt ttctctcttg aatctcatgt aaagtcactt
     1381 taaatacagc atcttttcca gctaaatctt ttgcttgata atcttttggg aattgaacag
     1441 taatatcttt ttgctcttca tatttcatac caataacttg ttcttcaaat cctggaataa
     1501 atgaaccaga acctatttgt agtggatatt tttcagcttt tccaccttca aatgcaacac
     1561 catcaacaaa accttc
//
LOCUS       DAOQAN010000326         1572 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_123138_length_1572_cov_2.787739, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000326 DAOQAN010000000
VERSION     DAOQAN010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123138_length_1572_cov_2.787739"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="PKO10_05505"
     CDS             complement(<1..328)
                     /locus_tag="PKO10_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014472997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HNW66635.1"
                     /translation="MKKIFDEVRSLDKRAIEEFHLTEDILMENASLGLKNYITKKFKK
                     NSSILIVCGSGNNGADGISLARLLQNKFEVSLYLINEPKTEIGKLQLKRAKSINVNFV
                     NQIFQAD"
     gene            complement(352..708)
                     /locus_tag="PKO10_05510"
     CDS             complement(352..708)
                     /locus_tag="PKO10_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014472996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HNW66636.1"
                     /translation="MGIVEKLSLGLGAKLEGNSFDTQKDDKAKVKVEKRISKVVLPKN
                     QHQLVFSLEKRNGKPVTIIGKFQIEDEKIKDILRLLKTKLACGGSIKDEYIEIQGDLK
                     DKVRVILVDNGWKFKN"
     gene            complement(710..>1572)
                     /gene="tsaD"
                     /locus_tag="PKO10_05515"
     CDS             complement(710..>1572)
                     /gene="tsaD"
                     /locus_tag="PKO10_05515"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HNW66637.1"
                     /translation="LAARLHIEALPKILEECKEYLKDLKAVAVTNAPGLSVTLTEGVS
                     MAKAISIALNIPIIAVNHLKGHIYSLFIEKDAIFPMTTLLVSGGHTQIIEANSLNDMK
                     ILGKTLDDSFGESFDKVSKMLKLGYPGGPIVEKYAKNGDENRFSFPIPLSQSPNIEFS
                     YSGLKNAVRLEIERLERTTLEEQDISDICACFQKTAVKHILQKLKKLFKQKTIKNFAI
                     VGGASANLYLRKELEKICLEYKSTLHLSDLMYCSDNAAMIGRVAVEQYKQNDFINLED
                     LDIKSRLKDY"
BASE COUNT          482 a          274 c          166 g          650 t
ORIGIN      
        1 catctgcttg aaaaatttga tttacaaaat ttacatttat tgattttgct ctttttagtt
       61 gtaattttcc aatctctgtt tttggttcat taataagata taaactaact tcaaatttgt
      121 tttgaagcaa tcttgctaag gaaataccat ctgcaccatt atttccactt ccacaaacaa
      181 ttaaaataga actatttttt ttgaattttt ttgttatata attttttaga cctaaagaag
      241 cattttccat caagatatct tctgttaagt gaaattcttc tattgctctt ttatccaaac
      301 ttcttacttc atcaaatatc tttttcactg gaatactcct ttatttagtt gttagttttt
      361 aaatttccat ccattatcaa ccaaaataac tcttacttta tcttttaaat caccttgaat
      421 ttcaatatat tcatctttta ttgaacctcc acaagcaagt ttcgtcttaa gtaatcttaa
      481 aatatctttt attttttcat cttctatttg gaattttcca ataatagtaa ctggttttcc
      541 atttcttttt tcaagagaaa aaactaattg atgttgattt tttggtaaaa caacttttga
      601 aattctcttt tctactttta ctttagcttt atcatctttt tgtgtatcaa aactatttcc
      661 ttcaagtttt gcaccaagac ctaaagacaa tttttctact atacccattc tagtaatcct
      721 ttaatctact ttttatatct aaatcttcta gatttataaa atcattttgt ttatactgct
      781 caaccgcaac tcttcctatc atagcagcat tgtctgaaca atacattaaa tcacttaaat
      841 gcaaagttga tttatattcc aagcagattt tttcaagctc ttttcgtaaa tataaatttg
      901 cacttgcacc tccaactatc gcaaaatttt tgatagtttt ttgtttaaaa agttttttta
      961 acttttgcaa gatatgtttt acggctgttt tttgaaaaca agcacatata tcacttatat
     1021 cttgctcttc taaagtggtg cgttcaagtc tttctatttc gagtctaact gcatttttaa
     1081 gccctgaata actaaactct atatttggac tttgagaaag tggaattgga aaagaaaatc
     1141 tattttcatc tccatttttt gcatactttt caactattgg accaccagga taacccaatt
     1201 ttaacatttt tgaaacttta tcaaaactct caccaaaact atcatctaat gtttttccta
     1261 gaattttcat atcatttaga ctatttgctt caattatttg agtatgacct cctgaaacta
     1321 gaagagttgt cataggaaat attgcatctt tttctataaa aagtgagtaa atatgccctt
     1381 ttaaatggtt tactgcaatt attggaatat ttaaagctat acttattgcc tttgccatag
     1441 aaactccctc tgtaagagta acactaagac ctggtgcatt tgtaacagca acagctttta
     1501 aatcttttaa atactcttta cactcttcaa gaatttttgg taatgcttct atatgaagtc
     1561 tagctgcaag tt
//
LOCUS       DAOQAN010000327         1568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_123580_length_1568_cov_3.417052, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000327 DAOQAN010000000
VERSION     DAOQAN010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123580_length_1568_cov_3.417052"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /gene="mobB"
                     /locus_tag="PKO10_05520"
     CDS             complement(<1..358)
                     /gene="mobB"
                     /locus_tag="PKO10_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014474256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HNW66638.1"
                     /translation="MNKKRLVVAFSGPSNSGKTTAIVKVASILQDSDFKVCIIKHDPK
                     DKAMFDREGKDSFKFSQTGADVAVVSPNKTTIFKKDSSTIDELISIFNDFDYLLVEGL
                     KTLELPRISIFRNKLDD"
     gene            461..940
                     /locus_tag="PKO10_05525"
     CDS             461..940
                     /locus_tag="PKO10_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66639.1"
                     /translation="MKKGLFLVAISILFVACTPKNSAFRYFDKGDIETNAVRNTKKAD
                     IIKNDAIEVTFMATYLNKFDPKISPKEENFLIYIYFPNLEEQIDFTKNGYELLLNGKT
                     PISIEKLDRDDKKYEDMMLRNFWGSYYLVKFSNQELVQNLNITLKPKSSDATLNFEK"
     gene            complement(909..>1568)
                     /locus_tag="PKO10_05530"
     CDS             complement(909..>1568)
                     /locus_tag="PKO10_05530"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNW66640.1"
                     /translation="WLEVTEDSKKNLDDAKMEKYSNLFTQKSMEPHLAFILERYNRFK
                     NQYFITPYEDLLNNYGIYKKVLIYSIAKQESRFIPSSISFSSAMGIMQIMPFLSKDIA
                     SKLGDDYNIYDQFLPEKNIQYASFHLDSLIKQFDSNPLFIAYAYNGGAGYARSQLQKG
                     LFKDKSEFEPFLSMEMISYNETREYGKKVLANFYIYNNYLNSENKISLSSIFQNLVWH
                     H"
BASE COUNT          572 a          206 c          200 g          590 t
ORIGIN      
        1 tatcatctag cttatttcta aaaatagaga ttctaggaag ttctaaagtt ttaagtcctt
       61 caactagcag ataatcaaaa tcattaaaaa tagatattaa ttcatcaata gttgaactat
      121 cttttttaaa tatagttgtt ttattagggc ttacaactgc aacatctgct cctgtttgtg
      181 agaatttaaa agagtctttt ccctctctat caaacatagc tttatctttt gggtcgtgct
      241 ttattatgca cactttaaaa tcactatcct gcaaaatact tgcaacttta acaatagctg
      301 ttgttttacc actatttgat ggccctgaaa atgccactac taatcttttt ttattcatgg
      361 ttaggatttt atccaaagaa ttgttaaact ctatttatat aaaaatatat tttaaagtgt
      421 agtttttgta taatcgctac cttatatatg aggagtttta atgaaaaaag gtttattctt
      481 agttgcaatt tcaattttat ttgtagcttg tacaccaaaa aatagtgcat ttagatattt
      541 tgataaaggt gatattgaaa caaatgcagt tagaaataca aaaaaagctg atattataaa
      601 aaatgatgca atagaagtta catttatggc aacatattta aataaatttg atccaaaaat
      661 aagcccaaaa gaagagaatt ttttgattta tatctatttc ccaaatttag aagaacagat
      721 agattttact aaaaatggat atgaacttct tttaaatgga aaaactccaa tatctattga
      781 aaaattagat agagatgata aaaagtatga agatatgatg cttagaaact tttggggtag
      841 ctactattta gtaaaattta gtaatcaaga gttagtacag aatttaaata taactttaaa
      901 accaaaatct agtgatgcca cactaaattt tgaaaaatag aagatagtga gattttattc
      961 tcactattta aatagttgtt gtatatataa aaattagcta aaactttttt tccatactct
     1021 cttgtttcat tatatgaaat catctccata cttaaaaatg gttcaaactc tgatttatct
     1081 ttaaacaaac ctttttgcaa ttgacttcta gcatatccag ctccaccatt gtaagcatag
     1141 gcgataaaaa gaggattact atcgaattgt ttaattaatg agtcaagatg aaaacttgca
     1201 tattgtatat ttttttcagg taaaaattga tcataaatat tgtaatcatc acctaactta
     1261 cttgctatat cttttgataa aaatggcata atttgcatta ttcccatggc acttgaaaat
     1321 gagattgaag atggaataaa tctactttct tgttttgcaa tagagtagat taaaactttt
     1381 ttatatattc cataattgtt taataaatcc tcataaggag ttataaaata ttgattttta
     1441 aatctattat atctttcaag aataaaagct aaatgtggtt ccatactttt ttgagtaaaa
     1501 agatttgaat atttttccat ctttgcatca tctaaatttt ttttgctatc ttctgtaact
     1561 tctaacca
//
LOCUS       DAOQAN010000328         1568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_123670_length_1568_cov_1.878387, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000328 DAOQAN010000000
VERSION     DAOQAN010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123670_length_1568_cov_1.878387"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1297
                     /locus_tag="PKO10_05535"
     CDS             <1..1297
                     /locus_tag="PKO10_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008341139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HNW66641.1"
                     /translation="KQDEVYILPAATQFETAGSVTATNRSGQWRYEVVKPMYESKPDQ
                     EIMFELAKRLGFYEQLTAGMQKGKDFVWPEDATNEIARIIKTIGLTGWRAERLKKHTD
                     NWHMFDEISLAGYGDMAGEYYGLPWPCWTEKHSGSPNLYDISKPVKFGGMGFRQNFGL
                     EHEGQTLLTPKGSAPVGGVQDGGYPEITKENIESVLKITLTEDEKAKMGANWKVDKSN
                     LIVEKCLEAGIAPYGNAKARAKVWQWADPIPKHREPLHTPRTDLIKDYPSFADKKDHW
                     RVDTRYISEQTKQDWAKDFPTNLVTGRLVNLNGAGVENRASKYLARLTPEMFCDINPD
                     LAGRHGIRDGSMMWIHSPNGTKIKVKAKYSYSVTPDRVFLPFHFAGIMEGVDLSHRYR
                     TGTLPYAIGESANTVTNYGYDIVTQIPETKGGLCRVERA"
     gene            1308..>1568
                     /locus_tag="PKO10_05540"
     CDS             1308..>1568
                     /locus_tag="PKO10_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNW66642.1"
                     /translation="MSNNKINFARMKFYCDENRCIHCDGCSVACASAHELPVEISRRK
                     VVTVNEGKQGMEFSLSVACMHCADAPCEQVCPVDCFYIREDGI"
BASE COUNT          572 a          209 c          335 g          452 t
ORIGIN      
        1 taaacaagac gaagtttata ttttaccagc agcaactcag tttgaaacag caggttctgt
       61 aactgcaaca aatagaagtg gtcaatggag atatgaagtt gtaaaaccta tgtatgaatc
      121 aaaacctgat caagaaatca tgtttgaatt agcaaaaaga ttaggatttt acgagcaatt
      181 aactgctgga atgcaaaaag gtaaagattt tgtttggcca gaagatgcaa caaatgagat
      241 agctagaatt attaaaacta ttggattaac tggatggaga gctgaaagac ttaaaaaaca
      301 tacagataat tggcatatgt ttgatgagat ttcactagct ggttatggag atatggctgg
      361 agaatactat ggtttaccat ggccttgttg gacagaaaaa cactctggaa gcccaaatct
      421 ttatgatatt tcaaaaccag ttaaatttgg tggtatggga tttagacaaa actttggttt
      481 agaacatgaa ggacaaacac ttttaactcc aaaaggttca gcaccagttg gtggtgttca
      541 agatggtgga tatccagaga ttacaaaaga gaatattgaa tcagttttaa aaattacttt
      601 aactgaagat gaaaaagcta aaatgggtgc aaactggaaa gttgataaat ctaacttgat
      661 agttgaaaaa tgcctcgaag ctggaattgc tccttatgga aatgcaaaag caagagcaaa
      721 agtttggcaa tgggcagatc ctattccaaa acatagagag cctttacata ctccaagaac
      781 ggatttaatt aaagattatc caagttttgc agataaaaaa gatcactgga gagttgatac
      841 tagatatatt agtgaacaga caaaacaaga ttgggcaaaa gatttcccaa caaaccttgt
      901 aactggaaga ttagtaaatc taaatggtgc tggagttgaa aatagagcta gtaaatattt
      961 agctagatta acacctgaaa tgttctgtga tattaatcca gatttagcag gaagacatgg
     1021 tattagagat ggttctatga tgtggattca ttcaccaaat ggaacgaaaa ttaaagttaa
     1081 agcaaaatac tcttactctg ttacacctga tagagtattc ctaccattcc attttgctgg
     1141 aattatggaa ggtgttgatt taagccatag atatcgaact ggaacactac cttatgcaat
     1201 aggtgagagt gcaaatacag ttactaacta cggttatgac atagttacac aaatccctga
     1261 aacaaaaggt ggtttatgta gagtagaaag agcttaggag aagtaacatg agtaataata
     1321 agataaattt tgcaagaatg aagttctatt gtgatgaaaa tagatgtatt cattgtgatg
     1381 gttgttcagt tgcttgtgcc tcagcacatg agttaccagt tgagattagt agaagaaaag
     1441 ttgtaactgt aaatgagggt aaacaaggaa tggaattttc attgtctgtt gcttgtatgc
     1501 attgtgcaga tgcaccttgt gagcaggttt gtccagttga ttgtttctat atcagagaag
     1561 atggtatt
//
LOCUS       DAOQAN010000329         1568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_123671_length_1568_cov_1.876404, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000329 DAOQAN010000000
VERSION     DAOQAN010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123671_length_1568_cov_1.876404"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="PKO10_05545"
     CDS             <1..382
                     /locus_tag="PKO10_05545"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HNW66643.1"
                     /translation="IKGAYLKGCDDYIKKPFNIQELLFKLAKFKKNSNIFKFDDETLF
                     DMETKKFIYNNSEIELTKNEKNFLHLLICNQGKCVNYTLIEDVVYNGEFKSLDAIRSL
                     IKRLRKKLPKDIIFNNLDEGYFIK"
     gene            complement(387..656)
                     /locus_tag="PKO10_05550"
     CDS             complement(387..656)
                     /locus_tag="PKO10_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66644.1"
                     /translation="MERRDWSIKLLKELIYIDSLDSYEKANNLVSWYTEYFSKSSIND
                     LDLELSDLKILEELFYRNINFLKEQQKQAGEELKNIKKMKSFLKY"
     gene            complement(632..988)
                     /gene="fliS"
                     /locus_tag="PKO10_05555"
     CDS             complement(632..988)
                     /gene="fliS"
                     /locus_tag="PKO10_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014473063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HNW66645.1"
                     /translation="MGIELYNQQNAVSDDPYALVLKLYEGIIKYLSFAKSAINNGDVE
                     NKFTYINKSIAIFDELRNVLDFDGGEVAYYLDGLYLYQIETLFSAGVDDNINSINQVI
                     KVTQGLIDAWKEETGL"
     gene            complement(1077..>1568)
                     /gene="fliD"
                     /locus_tag="PKO10_05560"
     CDS             complement(1077..>1568)
                     /gene="fliD"
                     /locus_tag="PKO10_05560"
                     /inference="COORDINATES: protein motif:HMM:NF018852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HNW66646.1"
                     /translation="NEIYSADASVDNKGALRDMLATVKNELFGTGSGKQSIFDFGFSL
                     DEKSGDIIFNKKDFEASIKNGTADFEALFAGTADKKGIATSMDELISTNGVTKSLLDY
                     EISMLEREERLKKDKETAETTLDNKYSLMAQQFASYGVMINQMESSFSGLKMLILQSQ
                     ASR"
BASE COUNT          554 a          245 c          176 g          593 t
ORIGIN      
        1 cataaaaggt gcttatctaa aaggatgtga tgattatatt aaaaagcctt tcaatattca
       61 agaattactt ttcaagcttg caaaatttaa gaaaaatagt aatattttca aatttgatga
      121 tgagacacta tttgatatgg aaacaaaaaa attcatatac aataatagtg agattgaact
      181 cacaaaaaat gaaaagaatt ttcttcacct tctaatatgt aatcaaggaa aatgtgtaaa
      241 ctacacttta atagaagatg tagtatataa tggtgaattt aaaagcttag atgctattag
      301 aagtttgata aaaagactta gaaaaaagct tccaaaagat attatattta acaatcttga
      361 tgaggggtat tttataaaat agttttttaa tattttaaaa aactcttcat tttttttata
      421 ttttttaact cttcgcctgc ttgtttttgt tgctctttta aaaagtttat atttctataa
      481 aataactctt ctaatatttt taagtcactt aattccaaat ctaaatcatt tatactagat
      541 tttgaaaaat actctgtgta ccaagaaacc aaattatttg ctttctcgta agaatctaaa
      601 gagtcgatat aaatcaactc ttttaaaagt tttatagacc agtctcttct ttccatgcgt
      661 cgattaatcc ttgtgtaact ttaataactt gatttattga attaatatta tcatcaactc
      721 cagcactaaa aagagtctct atttgataca aatataaacc atctaaatag tatgcaactt
      781 ctccaccatc aaaatcaagt acatttctaa gttcatcaaa tattgcaatt gacttattta
      841 tatatgtaaa tttattttca acatcaccat tatttattgc actttttgca aatgatagat
      901 actttatgat accttcataa agttttaaaa ccaaagcata agggtcgtct gatactgcat
      961 tttgttggtt atacagctca atccccataa aatttaactc caaaaacaat ttattttagt
     1021 atattatata tttacttaaa tgcaagatgg cttaaaagcc acctttttaa agactcctat
     1081 ctagaagctt gtgattgtaa aataagcatt tttaaaccag aaaatgatga ttccatttgg
     1141 tttatcataa ctccatatga agcaaattgt tgtgccatta aagagtattt attatcaaga
     1201 gttgtttctg cagtttcttt gtcttttttt aatctctctt ctctttctag catacttatc
     1261 tcataatcta gaagactttt tgtaacccca tttgtactaa ttaactcatc catagatgta
     1321 gcaatacctt ttttatcggc tgttcctgca aataaagctt caaagtcggc tgttccattt
     1381 tttattgaag cttcaaaatc ttttttatta aaaattatat caccactttt ttcatctagt
     1441 gaaaatccaa aatcaaatat agattgcttt ccagaacctg ttccaaaaag ttcatttttg
     1501 acagttgcta acatatctct taatgcacct ttgttatcaa cactagcatc agcactataa
     1561 atctcatt
//
LOCUS       DAOQAN010000330         1562 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_124331_length_1562_cov_3.156603, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000330 DAOQAN010000000
VERSION     DAOQAN010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124331_length_1562_cov_3.156603"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="PKO10_05565"
     CDS             complement(<1..452)
                     /locus_tag="PKO10_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008336363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HNW66647.1"
                     /translation="MNIFDAIWLGIIEGVTEFIPVSSTGHLIVLSEFLGIEQNNVNKA
                     FEIIIQFAAIMALVFIYPSKFTFKHINLWMKIALAFLPIGIVGFIFSSQIKALFSIEI
                     VAWMFIIGGVIFLIVEKFYDENKKHITDVEDVSYKQALFIGIAQIAAL"
     gene            complement(449..1114)
                     /locus_tag="PKO10_05570"
     CDS             complement(449..1114)
                     /locus_tag="PKO10_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014469251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66648.1"
                     /translation="MIFSKIDFINLLPFHIYIKKNIKSNQLKSSIEYKKSYPSKITNS
                     FKKRKTHSAFISSIGSRNEKFLDFGIIARDYVDSVLILPNRKNKDDFQSKTSNALAKV
                     LELDGEVIIGDKALKFYHDSKDEKFIDLAKQWQNRYNLPFVFSVLCYNKYGKRLKKLT
                     KNFDKRKIKIPQYILEQYSKRSGISKKNILDYLTKIDYDIGYKEKRALKLFLKLTKQK
                     GII"
     gene            1185..1406
                     /locus_tag="PKO10_05575"
     CDS             1185..1406
                     /locus_tag="PKO10_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002781225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNW66649.1"
                     /translation="MVKVEFLGPINKESIDLDIKNLSELSNILKNDEKISSWLETCAV
                     AVNDTLVFSKNFELKSGDKISLLPPVCGG"
BASE COUNT          562 a          207 c          198 g          595 t
ORIGIN      
        1 attagtgcag ctatttgagc aataccaata aataaagctt gtttgtaact cacatcttca
       61 acatctgtta tatgtttttt attctcatca taaaacttct caactattaa aaatataact
      121 ccaccaataa taaacatcca agctacaatc tctatagaaa agagagcttt tatttggctt
      181 gagaaaataa atccaacaat tcctataggt aaaaatgcta gtgcaatttt catccataaa
      241 tttatatgtt taaatgtaaa tttagatgga tatataaaaa ccaaagccat aatagcagca
      301 aattgaataa taatctcaaa agctttattt acattgtttt gttcaattcc caaaaactca
      361 cttaaaacta ttaagtgtcc cgttgatgat actggtataa actcagtaac tccctctata
      421 attcctaacc aaattgcatc aaatatattc aaataatccc cttttgtttt gtaagtttta
      481 aaaatagttt taaagctcgt ttctctttat acccaatatc ataatcaatt tttgtgagat
      541 aatccaaaat atttttcttt gaaataccac ttctttttga gtactgctct aaaatatatt
      601 gaggaatttt gatttttctt ttatcaaaat tttttgtaag tttttttaat cttttcccat
      661 atttattgta acacaaaact gaaaatacaa aaggaagatt gtatctattt tgccactgtt
      721 tagctaaatc tataaatttt tcatctttac tgtcatgata aaactttaaa gccttatctc
      781 caatgattac ttcgccatct agttctaaaa cttttgctag tgcatttgaa gtttttgatt
      841 gaaaatcatc tttatttttt ctatttggta agattaaaac agaatccaca taatcccttg
      901 caattattcc aaaatcaaga aatttctcat ttcttgaacc aattgaagag ataaaagcac
      961 tgtgagtttt tcgtttttta aaactatttg taatttttga tggataagat tttttgtatt
     1021 caatacttga cttaagttga tttgatttta tatttttttt gatataaatg tgaaaaggta
     1081 ataggtttat aaaatcaatt tttgaaaata tcataaaatt gttgccttta gtctttaaaa
     1141 ttaaagttga tataatagta taaaaagaat tagaaggtat taaaatggta aaagtagagt
     1201 tcttaggacc tataaataaa gagagtatag atttagatat aaaaaattta agtgagttgt
     1261 caaatatttt gaaaaatgat gaaaaaatat cttcttggct tgaaacttgt gcagttgctg
     1321 tgaatgatac tttagttttt tcaaaaaact ttgagctaaa gtctggtgat aaaatttcac
     1381 ttcttcctcc tgtttgtggt ggttgaaatg tttacgaaag atttacaaat acacaaaggt
     1441 aatttaccaa tagaaaaaat tcacaactct tggtatgaaa agtataaaaa tttaaattat
     1501 ggtgcgttta taacttttgt tggcatagta agagatgaaa atggcataga tggtctatct
     1561 tt
//
LOCUS       DAOQAN010000331         1557 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_124995_length_1557_cov_2.591212, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000331 DAOQAN010000000
VERSION     DAOQAN010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124995_length_1557_cov_2.591212"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(127..1155)
                     /gene="pyrC"
                     /locus_tag="PKO10_05580"
     CDS             complement(127..1155)
                     /gene="pyrC"
                     /locus_tag="PKO10_05580"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011980083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HNW66650.1"
                     /translation="MEKTFILNEPLDMHLHLRDGDMLRLVAPLTSNSFSGALVMPNLV
                     PPVTTKDALLSYKKRVIEACKGDDFTPYMTLFFQNNYSFEFLEDIKDEIIGIKLYPAG
                     ITTNSETGVSSMDIEVLRPTLENMSRLGIPLCIHGETNGFVMDREKEFMPIYESLAIA
                     FPNLKIVMEHITTKDAVELLDKYSNLYATVTLHHLLITLDDVAGGMLDPHLFCKPIAK
                     RPVDRNALLNAALKAHPKLMFGSDSAPHPKHKKECCGCAAGVFTSPIALQVLVELFEK
                     HDSLENLNDFVSNNAKKIYGLNLEPKTINLVKKDFIVPAIYEYKDEKVVPMYAGKTIS
                     WSIESIQK"
     gene            complement(1156..1494)
                     /locus_tag="PKO10_05585"
     CDS             complement(1156..1494)
                     /locus_tag="PKO10_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HNW66651.1"
                     /translation="MKKIEAIIKPFKLEDVKDALTQAGISGMTVLDVKGYGRQQGHSE
                     LYRGAEYVVDFLPKIKLELIVADEDVDKIITVITEAAKTGNIGDGKIFVSSIEKIIRI
                     RTGEQDEEAI"
BASE COUNT          540 a          259 c          190 g          568 t
ORIGIN      
        1 actcttgaaa ctttaagatt gatgagagaa gagagagaac agaaggcaaa agagaatgct
       61 gaaaatatta aaaaattaat aaatgaaaga agaaattcta atttttctta ataggctaaa
      121 tttagcctat ttttgaatac tctctatact ccaagagata gttttacctg catacattgg
      181 tacaactttt tcatctttat actcataaat agcaggaact ataaaatctt tttttactag
      241 atttatagtt tttggctcta aatttaatcc ataaattttt tttgcattat ttgatacaaa
      301 atcatttaaa ttttctaaac tatcatgttt ttcaaaaagt tcaactaaaa cttgtaaagc
      361 aattggtgat gtgaaaactc ctgctgcaca accacaacac tcttttttat gttttggatg
      421 aggagcacta tcacttccaa acataagttt tggatgagct tttaaagctg catttaaaag
      481 tgcatttcta tcaactggtc tttttgctat tggtttacaa aaaagatgag gatctagcat
      541 tccacctgct acatcatcta aagttattaa caaatgatgt aaagtaactg ttgcataaag
      601 atttgaatac ttatcaagca attcaactgc atcttttgtt gtaatatgct ccattacgat
      661 ttttaaatta ggaaaagcaa tagccaaact ttcataaatt ggcataaact ctttttctct
      721 atccattaca aaaccatttg tttcaccatg aatacaaaga ggaattccaa ggcggctcat
      781 attttctaaa gtaggtctta aaacttcaat atccattgaa gaaactcctg tttcagagtt
      841 tgtagtaatt cctgctggat aaagtttaat tccaataatc tcatctttaa tatcttctaa
      901 aaactcaaaa ctatagttat tttgaaaaaa aagtgtcata tatggagtaa aatcatcacc
      961 tttacaagct tctataactc tttttttata agataataaa gcatctttag tagtaacagg
     1021 agggactaaa tttggcataa ccaaagcacc actaaaacta tttgatgtaa gaggggcaac
     1081 aagcctaagc atgtcgccat ctcttaaatg taagtgcata tccaaaggtt catttaaaat
     1141 aaatgttttt tccatttaaa tagcttcctc atcttgttca cctgttctaa ttcttatgat
     1201 tttttcaata gatgaaacaa atattttacc atcaccaata tttccagtct ttgcagcttc
     1261 tgtgattact gttattattt tatcaacatc ttcatcagca actattaact ctaatttaat
     1321 ctttggtaaa aaatcaacaa catattcagc acctctatat agttcgctgt gcccttgttg
     1381 tcttccataa cctttaacat ctaatacagt catacctgaa attccagctt gagttaatgc
     1441 atctttaaca tcttcaagtt taaatggttt aattatagct tctatttttt tcaatattaa
     1501 tcctttcttt aattcaatta ttttaacaaa attttattaa actaatatta ttagaat
//
LOCUS       DAOQAN010000332         1556 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_125141_length_1556_cov_2.033311, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000332 DAOQAN010000000
VERSION     DAOQAN010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125141_length_1556_cov_2.033311"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..834
                     /locus_tag="PKO10_05590"
     CDS             <1..834
                     /locus_tag="PKO10_05590"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNW66652.1"
                     /translation="DKIANPSRLLKVIQSVNSLGDTLATQAKNSLENGSSLETNSKTL
                     KNSIENLTSKITKQIESLEETTLAVEKISEITNNNSKNTTSMSKLAEIVKQAVEDGYN
                     LSNKTTKSMDDINEKVIAINEAITIIDQIAFQTNILSLNAAVEAATAGEAGKGFAVVA
                     GEVRNLANKSAEAANEIKKLVEIANQKAHEGKDISNEMQNGYKNLHTHITETLQIIQN
                     VSDAANEQMVGINQVSQTIVSLDQISKDNQKETNLINDISNVVSSMAIEVLEDAKNKK
                     F"
     gene            866..1267
                     /locus_tag="PKO10_05595"
     CDS             866..1267
                     /locus_tag="PKO10_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNW66653.1"
                     /translation="MKEEETILDDYAFLVSETDEKGIIRFANRDFCNIAEYNLEELIG
                     KPHNIVRHKDMPKVAFKDLWETVAKGNIWTGYVKNATKSGGYYWVFATVYPFSSCDGT
                     KGYLSCRRRASKDEIEKISKLYEVWINEEKK"
     gene            1340..>1556
                     /locus_tag="PKO10_05600"
     CDS             1340..>1556
                     /locus_tag="PKO10_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66654.1"
                     /translation="MSELINCPSCNNKILSRMGTICPNCKYTVGYFNGEKRRKGYGRL
                     FALTIFAPFFSFFTLVFAQINFYSFILA"
BASE COUNT          657 a          181 c          261 g          457 t
ORIGIN      
        1 gataaaattg caaatccatc aagactttta aaagttattc aaagtgtaaa ctctttaggt
       61 gatactttag caacacaagc aaaaaatagt ttagaaaatg gtagttcttt ggagactaac
      121 tctaaaactt taaaaaactc tattgaaaat cttacttcaa agattacaaa acagattgaa
      181 agtcttgaag agacaacctt agctgtagaa aaaatatcag aaataacaaa taacaactca
      241 aaaaacacta cttctatgtc aaaattagca gaaattgtaa aacaagctgt tgaagatggt
      301 tacaatctat caaacaaaac aactaaatca atggatgata taaatgaaaa agttatagct
      361 ataaatgaag ctataactat aatagaccaa atagcatttc aaacaaatat tctttcatta
      421 aatgcagcgg tagaagcagc aactgctggt gaggctggaa aaggttttgc tgttgttgca
      481 ggagaagtta gaaatctagc aaacaaaagt gcagaagctg caaatgagat aaaaaaatta
      541 gtagaaatag caaatcaaaa agcgcatgaa ggtaaagata tatcaaacga gatgcaaaat
      601 ggttacaaaa atcttcacac tcatattact gaaactttac aaattatcca aaatgtaagt
      661 gatgcagcaa atgagcagat ggttggaata aatcaagtaa gtcaaactat agtaagctta
      721 gaccaaatat caaaagataa tcaaaaagag acaaatttga taaatgatat ttcaaatgta
      781 gtatcatcta tggctataga agttttagaa gacgcaaaaa ataaaaaatt ttaaaatata
      841 attaaaatat aggggtattt atattatgaa agaagaagag acaattttag atgattatgc
      901 ttttttggtt agcgaaactg atgaaaaagg tattataagg tttgcaaata gagatttttg
      961 taatatcgct gaatataatt tagaagagct aatagggaaa cctcacaata ttgtaagaca
     1021 taaagatatg ccaaaagttg ctttcaaaga tttgtgggaa actgttgcaa aaggtaatat
     1081 ttggactgga tatgtaaaaa atgcaacaaa aagtggtgga tattactggg tttttgcaac
     1141 agtttaccct ttttcatctt gtgatggaac aaagggatac ttatcttgta ggagaagagc
     1201 atcaaaagat gagattgaaa aaatatcaaa gctatatgaa gtttggataa atgaagaaaa
     1261 aaaataaaaa ttttattctt taaacttttg ttgattaaaa aacttatgga tataatgcaa
     1321 caaaaaatta ggaatatata tgtctgaact tattaattgc ccaagttgta ataacaaaat
     1381 tctatcaaga atgggtacaa tatgtccaaa ttgtaaatat acggttggat atttcaatgg
     1441 agagaaaaga agaaagggtt atggaagact ttttgcactt acaatatttg caccattttt
     1501 ctcattcttt acactagttt ttgctcaaat taatttttat agttttattt tagcag
//
LOCUS       DAOQAN010000333         1555 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_125230_length_1555_cov_2.755333, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000333 DAOQAN010000000
VERSION     DAOQAN010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125230_length_1555_cov_2.755333"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            133..486
                     /gene="acpS"
                     /locus_tag="PKO10_05605"
     CDS             133..486
                     /gene="acpS"
                     /locus_tag="PKO10_05605"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HNW66655.1"
                     /translation="MIGIDIVSIDRIKKMYEKFGDKFYDRFLCEDEKEFIKSPQNAAG
                     FWAAKEAASKAIGVGIGEICSFYDIKIKKDKNNAPKIKYSKKLKKRFKIKKSSISITH
                     DGGFAIAVVVNSLKK"
     gene            647..1234
                     /locus_tag="PKO10_05610"
     CDS             647..1234
                     /locus_tag="PKO10_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013134357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66656.1"
                     /translation="MNIDFFQDVETIILKDKLGRFLGVDDDFVYTFQDVVKLSGHSCP
                     TVAGAYLMTIKALKELYKDELPVRGEIRVELRDSKSSGTTGVLANVASFITGAKEEDG
                     FKGLQGQYFRNNLLKYEAPIKGDMRFTRVDNQEKIEVLYDLSNISLSGFDGNLMQKGL
                     QKIATKEELEIFGSAWQKRVSEILLKYKDEVIKFI"
     gene            1245..>1555
                     /locus_tag="PKO10_05615"
     CDS             1245..>1555
                     /locus_tag="PKO10_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014770162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HNW66657.1"
                     /translation="MTLFILSTKPYDGSDNIYGALRLVKKLVEMKQEVKIFCISDAVD
                     LVRAIAKKPDSYDIDLQAMIKEILTLGVQIKACGTSLKRSGDYKNEPYLNEDIRGSLD
                     L"
BASE COUNT          622 a          142 c          291 g          500 t
ORIGIN      
        1 caaaaaatga gttaggagct aaatttatag caataaaaga tattaggcta ataataaaca
       61 gcataatgat aagatataat agttttttta taaatatatt catagttaag attatacaaa
      121 agataggatt aaatgatagg tatagatata gttagcatag atagaataaa aaagatgtat
      181 gaaaagtttg gtgataaatt ttatgataga tttttgtgtg aagatgaaaa agagtttata
      241 aaatctcctc aaaatgcagc tggtttttgg gctgcaaaag aggctgctag taaagctatt
      301 ggggttggaa ttggagagat ttgttcattt tatgatataa aaattaaaaa agataagaac
      361 aatgctccaa agattaaata tagtaaaaaa ttaaaaaaaa gatttaaaat taaaaaatca
      421 agtataagta tcacacatga tggtggtttt gcaatagcag ttgtagtaaa tagtttaaaa
      481 aaataaagtt tgatataaat caacaaaaga gtctaaataa tatattagaa tttctttact
      541 ctaacataga gcttgtacga gtggacttgc ccctaaatct ctcctaataa gtccaccgta
      601 caattttgtt ctatcaaatt ataaaaatca aaataaggta aattatatga atatagattt
      661 ttttcaagat gttgaaacaa ttattttaaa agataaatta ggaagatttc ttggtgttga
      721 tgatgatttt gtatatactt ttcaagatgt tgtaaaacta agcggtcatt cgtgtccaac
      781 agttgctgga gcttatctta tgactataaa agctttaaaa gagttataca aagatgagtt
      841 accagttcgt ggtgaaataa gagttgagct aagagattca aaaagttctg gaacaactgg
      901 agttttagca aatgtagctt catttataac aggagcaaaa gaggaagatg gatttaaagg
      961 acttcaaggg caatacttta gaaataatct acttaaatat gaagcaccaa taaaaggtga
     1021 tatgagattt acaagagttg ataatcaaga aaaaattgaa gttctctatg atttatcaaa
     1081 tatctctttg agtggttttg atggaaattt gatgcaaaaa ggtttacaaa aaattgctac
     1141 aaaagaggag cttgaaattt ttggctctgc ttggcaaaaa agagttagtg agattttatt
     1201 aaaatataaa gatgaagtta taaaatttat ataaaggagc ttttatgacg ctatttattt
     1261 taagtacaaa accatatgat ggaagcgata acatttatgg tgctttgaga ttagttaaaa
     1321 agttagtcga gatgaagcaa gaagtaaaaa ttttttgtat tagtgatgct gttgatttgg
     1381 tgcgtgcaat tgcaaaaaaa cctgatagtt atgatataga tttacaagct atgataaaag
     1441 agattttgac tttaggtgtg cagataaaag cttgtggaac aagtcttaaa aggtctggtg
     1501 attataaaaa tgaaccatat ttaaatgaag atataagagg aagcttggac ttgtt
//
LOCUS       DAOQAN010000334         1553 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_125515_length_1553_cov_2.132844, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000334 DAOQAN010000000
VERSION     DAOQAN010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125515_length_1553_cov_2.132844"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1167
                     /locus_tag="PKO10_05620"
     CDS             <1..1167
                     /locus_tag="PKO10_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013136380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNW66658.1"
                     /translation="VAALAFKIMTDPFVGQLTFTRIYRGILESGTYVLNSTKMKKERI
                     GRLLKMHANSREEVKELYAGEIGAVVGLKDTITGDTLASEKDPVILERMDFPEPVISV
                     AVEPKTKADQEKMGIALAKLAEEDPSFRVWTDEETGQTIIAGMGELHLEILVDRMKRE
                     FKVEAEVGAPQVAYRETIRNAVKQEYKYAKQSGGKGQYGHVYLEIKPMPAGSEPTFKF
                     NNEIKGGVVPKEYIPAVEKGAFEAMQGGILAGYPMVDIEVTLYDGSYHEVDSSEMAFK
                     LAASMGFKQGCRSAAAGAVLLEPIMKVEIETPEDYMGDVIGDCNKRRGQVQSMDDRAG
                     IKLVTAMIPLSEMFGYSTDLRSMSQGRATYSMIFDSYQEVPRNVSEEIMKKRNG"
     gene            1291..>1553
                     /locus_tag="PKO10_05625"
     CDS             1291..>1553
                     /locus_tag="PKO10_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HNW66659.1"
                     /translation="MRLDRSYKLLKKIENIGIILKPNSPELKDAYLKIKNLFENKNIN
                     IFLEEQSAKMIDASGLSFEDLCSKVDFLVSVGGDGTLLGVVRRA"
BASE COUNT          579 a          179 c          318 g          477 t
ORIGIN      
        1 gtagcagctc ttgcatttaa aattatgact gacccgtttg ttgggcaatt aacatttaca
       61 agaatttata gaggaatttt agagtctgga acttatgtat taaactctac aaaaatgaaa
      121 aaagagagaa tcggaagatt acttaaaatg catgcaaact caagagaaga ggttaaagaa
      181 ctttatgctg gagaaattgg tgcagttgtt ggtcttaaag atacaattac aggtgatact
      241 ttagcttctg aaaaagatcc agttattcta gaaagaatgg attttccaga accagttatc
      301 agcgttgctg ttgagccaaa aacaaaagct gaccaagaaa aaatgggtat tgctttagca
      361 aaacttgctg aagaagatcc atcatttaga gtatggactg atgaagaaac tggacaaact
      421 attattgctg gaatgggtga gttacacctt gaaattcttg tagatagaat gaaaagagag
      481 tttaaagttg aagctgaagt aggtgctcca caagttgcat atagagaaac tattagaaat
      541 gctgttaagc aagagtataa atatgcaaaa caatctggag gtaaaggtca atatggacat
      601 gtttacttag agattaagcc aatgccagct ggaagtgagc caacatttaa atttaataat
      661 gaaattaaag gtggggttgt accaaaagag tacattccag ctgttgaaaa aggtgcattt
      721 gaagctatgc aaggtggtat tcttgcaggt tatccaatgg ttgatattga agttactctt
      781 tatgatggaa gttatcacga agtggattca tctgagatgg cgtttaaatt agctgcttca
      841 atgggattca aacaaggttg tagaagtgca gctgcaggtg cagttttact tgagccaatt
      901 atgaaagttg aaatagagac tcctgaagat tatatgggag atgttattgg agattgtaat
      961 aaaagaagag gacaagttca atctatggat gatagagctg gtatcaaact tgttacggca
     1021 atgattccat tatctgaaat gttcggttac tctacagatt taagatctat gtctcaagga
     1081 agagcaacat attctatgat atttgactct taccaagaag ttcctagaaa tgtatctgaa
     1141 gagataatga aaaaaagaaa cggataatta tattttataa gtttcaaaaa aagggatgtg
     1201 cttttgcata tccctttttt attagtttta tatgagtaat ttactacaat tgcaaattat
     1261 ttatataaaa ttaaaaataa aagatttaaa ttgagattag atagaagcta taaattacta
     1321 aaaaagattg aaaatattgg aattatttta aaaccaaata gtccagaatt aaaagatgca
     1381 tatttaaaaa taaaaaatct ttttgaaaat aaaaatataa atatttttct tgaagaacaa
     1441 tcagcaaaaa tgatagatgc tagtggctta tcttttgaag atttgtgttc aaaagtcgat
     1501 tttttagtaa gtgtaggtgg agatggtact cttttaggag ttgtaagaag agc
//
LOCUS       DAOQAN010000335         1549 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_125956_length_1549_cov_3.073628, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000335 DAOQAN010000000
VERSION     DAOQAN010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125956_length_1549_cov_3.073628"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="PKO10_05630"
     CDS             complement(<1..378)
                     /locus_tag="PKO10_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66660.1"
                     /translation="MANLVDIFTQSSGLKGETTVLSTSEDLPKDKPSLFDSLLKTSIE
                     DIENSSAQNTNSKIVSQNITSNLEKSPLDAEVIEVNTKLIDEGLDSQAINLDENLNSE
                     EVIKDLSKNITNELNKDNTKKDEI"
     gene            complement(389..856)
                     /gene="flgC"
                     /locus_tag="PKO10_05635"
     CDS             complement(389..856)
                     /gene="flgC"
                     /locus_tag="PKO10_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014469276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HNW66661.1"
                     /translation="MGFFDGYNVSTSGMSAQRTRINVVSANIANAKTTHTQEGGPYKK
                     QQVVFEDVLLANKTRRTNSNDIEVNNSPQSDLALRAVGVKKIIHTDAQPVLRYDPTHP
                     DANEKGYVAYPDINPVIEMVDLIEAMRSYEANVTAFNTHRGIDTKTLDILAGN"
     gene            complement(872..1192)
                     /gene="fliE"
                     /locus_tag="PKO10_05640"
     CDS             complement(872..1192)
                     /gene="fliE"
                     /locus_tag="PKO10_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HNW66662.1"
                     /translation="MSIVGAINYLNPLQGIDSANVKQSETLSESQYADKSFKDLLNGA
                     INEVNDSQIEGYNSMKEIATGKVANLQEAVQKIEEAELSLKLGLEVKNKAINAYREIM
                     RMQI"
     gene            complement(1209..>1549)
                     /locus_tag="PKO10_05645"
     CDS             complement(1209..>1549)
                     /locus_tag="PKO10_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66663.1"
                     /translation="NTGIFKIYIAIGFFATIIFFTFNSELFSPLQMLFGAILVTVTLK
                     GFSNLMLSFIVNNFSLDQKRMEFDNRYNEDKINLLLNQLVVKDIKEDKENDEQSNENS
                     TQDKKEEAVS"
BASE COUNT          474 a          259 c          166 g          650 t
ORIGIN      
        1 tatctcatct ttttttgtat tatctttatt taactcattt gttatgtttt ttgataaatc
       61 ttttataact tcttcactat ttaaattttc atctaaatta atagcttgag aatctaaacc
      121 ttcatcaatc aattttgtat taacctctat aacttctgca tccaaaggac tcttttctaa
      181 atttgaagta atattttgag atactatctt tgaatttgta ttttgtgctg aactgttttc
      241 aatatcttca attgaagttt ttagaagaga atcaaataaa gatggcttat cttttggcaa
      301 atcttcactt gtgcttaaaa ctgttgtttc accttttaaa ccacttgatt gtgtaaaaat
      361 atcaactaga tttgccattt tttagtcctt aatttccagc taatatatct agagtttttg
      421 tatcaattcc cctatgagta ttaaatgcag taacatttgc ttcataagat ctcatagcct
      481 ctattaaatc aaccatttcg ataacaggat ttatatcagg atatgctaca taaccttttt
      541 cattcgcatc tggatgagta ggatcatatc ttaatacagg ttgcgcatct gtatgaataa
      601 tcttttttac tccaacagct ctaagagcta aatcagattg tggagaatta tttacttcaa
      661 tatcatttga atttgttctt ctagttttat ttgctaatag aacatcttca aaaacaactt
      721 gttgcttttt atatggtcca ccttcttgag tatgagttgt ttttgcattt gcaatattag
      781 cacttacaac atttattctt gttctttgtg cactcattcc tgaagttgat acgttatatc
      841 catcaaaaaa acccataatt tgctcctttt actaaatttg cattctcata atttctctat
      901 aagcatttat agctttattt ttaacttcta atcccaattt aagacttaat tctgcttctt
      961 caattttttg aacagcttct tgaagatttg caacttttcc tgttgcaatc tctttcatag
     1021 agttatatcc ttctatttga gaatcattta cctcatttat agccccattt aacaaatctt
     1081 taaaagattt atcagcatat tgactttcac ttaaagtttc actttgtttt acatttgcac
     1141 tatcaatacc ttgcaatgga tttaaatagt ttatagcacc tactatgctc attttttaat
     1201 ctcctatttt agcttacagc ttcttctttt ttatcttgag ttgaattttc atttgattgc
     1261 tcatcatttt ctttatcttc ttttatatct tttacaacaa gttgatttaa aagcaagttg
     1321 attttatctt cattatatct attatcaaac tccattctct tttgatctag actaaaatta
     1381 ttaactataa aagataacat taaattacta aaacctttta aagtaacagt aaccaaaatt
     1441 gctccaaaaa gcatttgaag aggtgaaaaa agttctgagt tgaatgtaaa aaatattatt
     1501 gtagcaaaaa aaccaattgc tatatatatc ttaaatattc cagtattta
//
LOCUS       DAOQAN010000336         1546 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_126384_length_1546_cov_2.406439, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000336 DAOQAN010000000
VERSION     DAOQAN010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126384_length_1546_cov_2.406439"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="PKO10_05650"
     CDS             complement(<1..418)
                     /locus_tag="PKO10_05650"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNW66664.1"
                     /translation="MKLFLLLLVSTFLYSSSLEKVSIQFNWKYQFEVAGFIAAKEKGF
                     YENVGLDVELKEYNPEVDILFDVLNNKVTYGISSSNIVLENKKIASIVLLVTYLQKSP
                     LVFITKPDIKTPSQFLGKTIMGNKDELKNSSLALFLS"
     gene            complement(455..1219)
                     /locus_tag="PKO10_05655"
     CDS             complement(455..1219)
                     /locus_tag="PKO10_05655"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNW66665.1"
                     /translation="MKRFYYLIVLFFIFNIYLFAQSDRSFVVIDANTNKVLIEEKSNK
                     KHKIASLTKIWTALIVLENSNLDDEVVVSKRATLQQGSSIYLKENQICTIKDLVHGML
                     LRSGNDASVALAEHIAGSEEKFVKLMNKRAKEFGIKNTKFVDVTGLGNNISTAKDVAI
                     MFELALKNSKFKDISGQSSYKNSLDGQIWKNKHKLVVENSKAFAGKTGYTKQSGRTLA
                     TAFYDEKSSKSFIVVTLNEKDDWKVHKSLAQKVFMK"
BASE COUNT          548 a          237 c          142 g          619 t
ORIGIN      
        1 gtgaaagaaa aagtgctaaa gagctatttt ttaactcatc tttatttccc ataatagttt
       61 ttcccaaaaa ttgtgaaggg gtttttatat ctggttttgt tataaaaact aaaggtgatt
      121 tttgtaaata agttaccaaa agaactattg aagctatctt cttattttct aaaactatat
      181 ttgaacttga tattccatat gtaactttat tatttaaaac atcaaaaagt atatctactt
      241 ctggattata ctctttaagt tcaacatcca aaccaacatt ctcataaaat cctttctctt
      301 ttgcagcaat aaatccagca acttcaaact gatatttcca attaaattga atagatactt
      361 tttctaaact tgatgaatac aaaaatgtgc ttactaacag taacaaaaat aatttcataa
      421 acaccctata atttatatac atttaaaact agttttattt cataaaaact ttttgagcca
      481 aagatttatg cactttccaa tcatcttttt catttaaagt aactactata aatgatttag
      541 aacttttttc atcataaaaa gcagtagcta aagtccttcc actttgtttt gtatatccag
      601 ttttacctgc aaaagcctta ctgttttcta caactagttt atgcttattt ttccaaattt
      661 gaccatctaa actattttta tatgaagatt gacctgatat atctttaaat ttgctatttt
      721 ttaaagccaa ttcaaacata attgctacat cttttgcagt tgatatattg tttccaagtc
      781 ctgtaacatc tacaaatttt gtatttttta taccaaactc ttttgctctt ttattcataa
      841 gttttacaaa tttttcttca cttccagcaa tatgttctgc caaagcaact gaagcatcat
      901 taccagagcg aagcaacatt ccatgaacca aatcttttat agtacaaatt tgattctctt
      961 ttaaatatat agatgaacct tgctgtaaag ttgctctctt tgaaactact acttcatcat
     1021 ctaagttact attttctaaa acaattaaag cagtccaaat ttttgtaaga cttgctattt
     1081 tatgtttttt gtttgatttt tcttctatta aaactttatt tgtattagca tctattacta
     1141 caaatgatct atcactttgt gcaaataaat aaatattaaa aataaaaaac aatactatta
     1201 aatagtaaaa tcttttcatc taataccttt tttattctaa aattatggat tctaacctag
     1261 tatataaaat cacttttttt attatatcta aattatttga attttatttt actagaaaaa
     1321 ataattttat ttatgttgaa aaataattat tatatggcta aaaagagttt taaaaaatag
     1381 tttatttaga gtaagtttaa taattttttg attttaaata ataaaaaaag agagtaaaaa
     1441 ttactctctt ttaaaaacga ttttagtttt ttaatttata aaaactaaat tattacatca
     1501 ttcctggcat tccgcccata ccacccattc cgctcatatc aggcat
//
LOCUS       DAOQAN010000337         1542 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_126833_length_1542_cov_3.075319, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000337 DAOQAN010000000
VERSION     DAOQAN010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126833_length_1542_cov_3.075319"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="PKO10_05660"
     CDS             <1..298
                     /locus_tag="PKO10_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66666.1"
                     /translation="ISSKEQIDLAFEEVINSKDSSYRLLFDTLGNNQKIALKIVGKYK
                     SGIFSQSVLKEFNIKKQTLQSSIEALFKSELIDKNDDRYFIPDRTFELWVERLV"
     gene            563..835
                     /locus_tag="PKO10_05665"
     CDS             563..835
                     /locus_tag="PKO10_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012083889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HNW66667.1"
                     /translation="MTTNLNKIRTNVYLDATTKEKAQEIFKQYGLGLSEAFNIFLTQS
                     VLQRGIPFEIKIPNDETLEAIKDARAKKNMSKVNLEDLKEDIKCLN"
     gene            823..1098
                     /locus_tag="PKO10_05670"
     CDS             823..1098
                     /locus_tag="PKO10_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HNW66668.1"
                     /translation="MLELEVHKIFTKDLIKAKLNPTNSAKLFLYISKLLNNEELPKEA
                     KNHFLGGEWSDTQEFHISGDLLVIYMISESSLQLLRIGTHSQLFKKF"
     gene            complement(1110..>1542)
                     /gene="murB"
                     /locus_tag="PKO10_05675"
     CDS             complement(1110..>1542)
                     /gene="murB"
                     /locus_tag="PKO10_05675"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014475400.1"
                     /note="catalyzes the reduction of UDP-N-acetylglucosamine
                     enolpyruvate to form UDP-N-acetylmuramate in peptidoglycan
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HNW66669.1"
                     /translation="EIFNYIHSIKTKDGYIKKENVDFSYRQTKIDTIVYEVVFHKTKG
                     FSKDMQNEFTKMRDNQPQIASAGSCFKNPKGDFAGRLIEAVGLKGYRIGDMEFSNTHA
                     NFLVNHENGTFDEAITLINLAKQKVKEQFDIELETEIIIFE"
BASE COUNT          568 a          205 c          206 g          563 t
ORIGIN      
        1 tatctcttca aaagaacaga tagatttggc ttttgaagag gtaataaact caaaagattc
       61 tagctataga ctactttttg atactttagg aaataatcaa aaaattgctt taaaaatagt
      121 tggaaaatat aaaagtggaa ttttctctca aagtgttctt aaagagttta atataaaaaa
      181 acagacttta caatctagta ttgaagcact ttttaaaagt gagttaatag ataaaaatga
      241 tgacagatat tttatccctg atagaacctt tgagctttgg gttgaaagat tggtttaaat
      301 cttatttttt gtttcatcat ttaaattttt atatttttgt agttgagttt tgatatgttc
      361 gattgtacta tttaagatct ttcaaaatta attctgataa aaaatagtta tactttatca
      421 tttcccccat ctacagcttg gaataaatac tttagtctaa actaaaatcc tgctcaaaaa
      481 atctagcaaa tcattttttt attcttaaag tattgacaaa aacacacaat gtgtgttaga
      541 atttattttt aaggaagtga aaatgactac aaatctaaat aaaataagaa caaatgtata
      601 tcttgatgca acaacaaaag agaaagctca agagattttt aaacaatatg gtttaggatt
      661 gagtgaagct tttaatattt ttttaactca aagtgttttg caaagaggta ttccatttga
      721 gataaaaatt ccaaatgatg aaacattaga agctataaaa gatgcaagag caaagaaaaa
      781 tatgtctaaa gttaaccttg aagatttaaa agaagatata aaatgcttga attagaagtt
      841 cataaaattt ttacaaaaga tctaatcaag gcaaaattaa atcccacaaa tagtgcaaaa
      901 ctatttttat atatttcaaa acttttaaat aatgaagagt taccaaaaga ggcaaaaaat
      961 cattttttgg gtggagagtg gagtgataca caagagtttc atatcagtgg agatttactt
     1021 gtaatttata tgataagcga aagtagtttg caacttctta ggattggaac tcactctcag
     1081 ctttttaaga aattttaaaa attataattt tactcaaaaa ttataatctc tgtttccaac
     1141 tctatatcaa actgctcttt tactttttgt tttgctagat ttatcaaagt tatcgcttca
     1201 tcaaatgtac cattttcgtg atttaccaaa aaatttgcat gagtatttga aaactccata
     1261 tctccaattc tatacccttt tagaccaact gcttctataa gtcgtcctgc aaaatcacct
     1321 tttggatttt taaaacagct tccagcacta gcaatttgtg gttggttatc tctcattttt
     1381 gtaaactcat tttgcatatc ttttgaaaaa cctttagttt tatgaaaaac cacttcataa
     1441 actatagtgt ctatttttgt ctgcctatat gaaaaatcta cattctcttt ttttatatat
     1501 ccatctttag tttttattga gtgaatatag ttaaaaatct cc
//
LOCUS       DAOQAN010000338         1542 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_126859_length_1542_cov_2.707465, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000338 DAOQAN010000000
VERSION     DAOQAN010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126859_length_1542_cov_2.707465"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="PKO10_05680"
     CDS             complement(<1..247)
                     /locus_tag="PKO10_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002849128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein"
                     /protein_id="HNW66670.1"
                     /translation="MKKNLVFKNKKAFHDFTILDSLEAGIMLEGSEVKAIREGRVNLK
                     DSFVRIIKGEVFLLNAHISHLSTTHSTYRPDERRSRKL"
     gene            complement(272..1039)
                     /locus_tag="PKO10_05685"
     CDS             complement(272..1039)
                     /locus_tag="PKO10_05685"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HNW66671.1"
                     /translation="MTKKSYAKVNIFLKIVGIRENYHLIASRFVRVKNLFDTISFIKK
                     DVDNFSIEGNFSCILEKNTVYKAYKELEKFDEVKEFFKKNIVKIDKNIPEFAGLGGGS
                     SNCATFLNMVNIACNLNLNKEELAKIGSNIGADVPFFVYEFDSANVSGIGEIVEEFKE
                     ELINIEVITPKIACDTGKIYKNFREKFYKEENMQKVKDEVKELFATNSKDILEKFSIN
                     EANDLYFPALDLNSDLKKFEKDNWFFSGSGSSFFKIL"
     gene            complement(1032..>1542)
                     /gene="truB"
                     /locus_tag="PKO10_05690"
     CDS             complement(1032..>1542)
                     /gene="truB"
                     /locus_tag="PKO10_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014475283.1"
                     /note="catalyzes isomerization of specific uridines in RNA
                     to pseudouridine; responsible for residues in T loops of
                     many tRNAs; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HNW66672.1"
                     /translation="EYIPPKFSAKRVNGQKAYEIAREGLEFELKSSIMKVFDIKFIRY
                     NHPFISFEVKVSEGSYIRSLAQIFLKNIACVGTLSYLERVSEGKFYFENHKELNPLDF
                     IDLPKNNYTGTKEWLEVGKRISKDYLSIKENGKYIIELDTFFSIIEIIDGEVKYLLNK
                     VSKIEAKND"
BASE COUNT          498 a          258 c          128 g          658 t
ORIGIN      
        1 gcagttttct tgatcttctt tcatctggtc tgtatgtact atgagttgta cttaaatgtg
       61 atatatgtgc atttaaaaga aaaacttccc ctttaattat tcgtacaaaa ctatctttta
      121 agtttactct tccttcacgt atagctttta cttcgcttcc ttcaagcata attccagctt
      181 caaggctatc taaaattgta aaatcatgaa atgctttttt atttttaaat actaaatttt
      241 tttttatctc tttttttgcc acataatttc cttataaaat tttgaaaaat gagcttccac
      301 ttccactaaa aaaccaatta tctttttcaa atttctttaa atcgctattt aaatccaaag
      361 ctggaaaata taaatcattt gcttcattta tactaaattt ttctaaaata tcttttgaat
      421 ttgtagcaaa aagctcttta acttcatctt tgactttttg catattttct tctttataaa
      481 atttttctct aaagttttta tatatttttc ctgtatcaca agcaattttt ggtgtaataa
      541 cttcaatatt aattaactct tctttaaact cttcaacaat ttcaccaatt ccactaacat
      601 ttgcactatc aaattcatat acaaaaaatg gtacatctgc tccaatattt gagccaattt
      661 ttgctaattc ttctttattt aaattcaaat tgcatgcaat atttaccata tttaagaaag
      721 tagcacaatt tgaacttcca ccaccaagtc ctgcaaattc tggaatattt ttatctattt
      781 ttacaatatt ttttttaaaa aactctttta cttcatcaaa tttttcaagt tctttataag
      841 ctttataaac tgtattcttt tctaaaatac atgagaaatt tccctctatt gaaaaattat
      901 caacatcttt ttttataaaa ctaatagtat caaaaagatt ttttactctt acaaatcttg
      961 aagctattag gtggtaattt tctcttattc caacaatttt taaaaaaata tttacctttg
     1021 catatgattt tttagtcatt ttttgcctct atttttgaaa ctttatttaa taaatattta
     1081 acttcaccat ctattatttc tataatagaa aaaaaggtat caagctctat gatatatttt
     1141 ccattctctt ttatactcaa gtaatctttt gaaattcttt taccaacttc taaccactct
     1201 tttgttccag tatagttatt ttttggtaaa tctataaaat caagtggatt taactctttg
     1261 tgattttcaa aataaaattt tccttcacta actctttcaa gataactcaa agttccaacg
     1321 caagctatat tttttaaaaa tatttgtgct aaagaacgaa tgtatgaacc ttcactaact
     1381 tttacttcaa aacttataaa aggatgattg tatctaataa attttatgtc aaataccttc
     1441 ataattgagc tttttagctc aaattctaat ccttctcttg caatttcata agctttttgt
     1501 ccatttactc ttttagctga aaattttgga ggtatatact ct
//
LOCUS       DAOQAN010000339         1542 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_126890_length_1542_cov_2.251513, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000339 DAOQAN010000000
VERSION     DAOQAN010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126890_length_1542_cov_2.251513"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="PKO10_05695"
     CDS             <1..511
                     /locus_tag="PKO10_05695"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNW66673.1"
                     /translation="EKANDSLSYLYNNMEYELKKEFENIDLEDFYLLDALKSSLDKFE
                     DIKKDTIIELFVPNNVVLHSDYNGFIIVLDNLISNAIKYNSKENPYIKISFENKVLSI
                     FNKGVGIDTKNIMLVFDRYFQENSQNIGYGIGLAVVKEFCDKNGIGINIESFEDGNRI
                     NLNMKSILN"
     gene            complement(503..1015)
                     /locus_tag="PKO10_05700"
     CDS             complement(503..1015)
                     /locus_tag="PKO10_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014473827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H"
                     /protein_id="HNW66674.1"
                     /translation="MQKIELFTDSSVNPKAKVGFAAFLVKNDKDIILESLNKSIKIKK
                     FENTSSTRIELQSLLWAINEIEKEKNDLSNVLIEVYTDCQNIVNLKNRKSKLVENDYQ
                     SGTGKLLKNHDLYKDFFEKESRLNLNIIKVKGHKKTILKDNIDHIFNLVDKASRSALR
                     DEFKEKESLI"
     gene            1151..1540
                     /locus_tag="PKO10_05705"
     CDS             1151..1540
                     /locus_tag="PKO10_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008335429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66675.1"
                     /translation="MAIFALQYLAGGFLDEDLQHFNKKFDDWCVSFDNYEDALTLAKT
                     LPNFENIDIVEITPLSYPKYFFSELQGTIYATRQIEDNIICVVEPFIGSSFRIAVCNL
                     KTKKVRFLKTHYKTIPSIEVAFANFKE"
BASE COUNT          554 a          161 c          204 g          623 t
ORIGIN      
        1 agaaaaagca aacgatagtc taagctactt gtataataat atggagtatg aattaaaaaa
       61 agagtttgaa aatatagatt tagaagattt ttatttgctt gatgcgttaa agagctcttt
      121 agataaattt gaagatataa aaaaagatac aattattgaa ctttttgttc caaataatgt
      181 agttttacat agtgattaca atggttttat aatagtttta gataatctta tttctaatgc
      241 tattaaatat aatagtaaag agaatccata tatcaaaata tcatttgaaa ataaggtatt
      301 atcaattttt aataaaggtg tgggaattga cacgaaaaat attatgttag tttttgatag
      361 gtattttcag gaaaattcac aaaacatagg atatggaata ggacttgctg ttgtaaaaga
      421 gttttgtgat aaaaatggta taggaataaa tatcgaaagc tttgaagatg gtaataggat
      481 aaatctaaat atgaagagta ttttaaatta aactctcttt ctctttaaat tcatctctta
      541 atgcacttct tgaagcttta tcaacaagat tgaaaatatg atcaatatta tcttttagaa
      601 tggttttttt gtgacctttt actttaataa tattaagatt taatctactc tctttttcaa
      661 aaaaatcttt atataaatca tgatttttta atagttttcc agttcctgat tgatagtcgt
      721 tttctactaa cttagatttt ctatttttta aattaactat attttgacaa tctgtataaa
      781 cttcaattaa aacatttgat aaatcatttt tctctttttc tatctcattt attgcccata
      841 ataaagattg aagttctatt cttgtagagc tggtattttc aaattttttt atttttatac
      901 ttttatttaa tgattctaag ataatatctt tatcgttttt aactaaaaat gcagcaaaac
      961 caacttttgc ttttggattt acgcttgaat ctgtaaaaag ttctattttt tgcaattttt
     1021 catctttata atttattatt tttaaagaga caagactaac atatttttgc tattaaaaag
     1081 ctcattactt gttatagttc attatgttag aataaacttt taaaattgta tataattaaa
     1141 aggtgtttat gtggctattt ttgctttaca atatttggcg ggtggatttt tagatgaaga
     1201 tttacaacat tttaataaaa aatttgatga ttggtgcgta tcttttgata attatgaaga
     1261 tgctttaact cttgccaaaa ctcttccaaa ctttgaaaat atagatattg tagagataac
     1321 tccattgagt tatccaaaat attttttctc tgagcttcaa ggtactattt atgcaacaag
     1381 acaaattgaa gataatatta tttgtgtagt agaaccattt attggttcaa gttttagaat
     1441 tgcagtatgt aatcttaaaa caaaaaaagt aagatttctt aaaactcatt ataaaactat
     1501 accaagtatt gaagttgctt ttgctaactt taaagagtaa at
//
LOCUS       DAOQAN010000340         1539 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_127276_length_1539_cov_1.989892, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000340 DAOQAN010000000
VERSION     DAOQAN010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127276_length_1539_cov_1.989892"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="PKO10_05710"
     CDS             complement(<1..394)
                     /locus_tag="PKO10_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66676.1"
                     /translation="MDKYANKYITFTINDDNGIKQIKLRKKPFFYTLYTFIGLFSIFV
                     AAFIFINLSLIQMDNDRLDIELELVEFKRINEELNNLVSQTQLELHEKNEEISEATDY
                     FNKMENIIGIPTENELPLKQRVDIARLNT"
     gene            complement(423..866)
                     /locus_tag="PKO10_05715"
     CDS             complement(423..866)
                     /locus_tag="PKO10_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HNW66677.1"
                     /translation="MSLKEKLNEDLKQAMRDKEVVKRDSIRAINTMIKQIEVDERRVL
                     DDAEVIKLVQRGIKQREEAISQYRAASRDDLVQKEQEQVDVFMLYLPKQLSNEELENG
                     MKEIISEVGATSLKDMGKVMGVASKKFAGVADGKRINEMVKKLLS"
     gene            complement(921..>1539)
                     /locus_tag="PKO10_05720"
     CDS             complement(921..>1539)
                     /locus_tag="PKO10_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNW66678.1"
                     /translation="KEYLDKKVGVLSGGEKNRVALALLFTKRVDCMILDEPTNDLDIP
                     TINILEEYLQNFQGALIFVSHDRYFVDKIAKKLFVFTGNGHIMESFQPYSEYLQIEKE
                     LKELDNLEVELEKEKTSVKVVNEPKKQTKLSYKDQREYDSLPNELEELEAKIEELNSC
                     LADPKCYEKIGIVARSKELEEKKAIYEEKVERFLTLEELVESFNS"
BASE COUNT          452 a          277 c          149 g          661 t
ORIGIN      
        1 ctgtattaag tcttgcaata tctactcttt gttttaatgg aagttcattt tcagttggaa
       61 ttcctataat gttttccatt ttattgaaat aatcagtagc ttcacttatc tcttcatttt
      121 tttcatgaag ttctagttga gtttgactga ctaaattatt tagctcttcg tttattcttt
      181 taaactctac taattcaagc tcaatatcta gcctatcatt atccatttga attaacgaaa
      241 gatttataaa aataaatgct gctacaaata tagaaaatag tccaataaat gtatataaag
      301 tataaaagaa aggctttttt cttagtttta tctgttttat accattatca tcatttattg
      361 taaatgttat atatttgttt gcatatttat ccattaatcc tcttaaactt taggtaatag
      421 tattatgata ataatttttt caccatctca tttattcttt ttccatcggc aacaccagca
      481 aactttttag aagcaactcc cataacttta cccatatctt ttaagctagt tgctccaact
      541 tcacttatta tctctttcat tccattttca agctcttcat tgcttaattg tttaggaaga
      601 taaagcataa aaacatcaac ttgttcttgc tctttttgaa ctaaatcatc tcttgaagca
      661 gctctatatt gagaaattgc ctcttctctt tgttttattc ctctttgaac aagttttata
      721 acttcagcat catctaaaac tcttctttca tctacttcta tttgtttaat cattgtattt
      781 atggctcgta tagagtctct ttttacaacc tctttatctc tcattgcttg ttttaaatct
      841 tcgtttaatt tctcttttaa gctcattttt aaactcctct tgtttagata taaatttttt
      901 tgaaactcga ttatactttt ttaagaatta aaactctcaa ctagttcttc aagagttaag
      961 aatctctcaa ctttctcttc ataaatagct tttttctctt caagctcttt tgaacgtgct
     1021 acaattccaa ttttttcata acattttgga tctgctaaac agctgtttaa ctcttctatt
     1081 ttagcttcaa gctcttcaag ctcatttggt aaactatcat actctctttg atctttatat
     1141 gataattttg tctgtttttt aggttcattt acaactttta cagatgtttt ctctttttct
     1201 aattcaactt ctaaattatc cagctctttt aactcttttt caatttgtaa atattcacta
     1261 tatggttgga aactctccat aatatgacca tttccagtaa atacaaaaag ttttttagca
     1321 attttatcta caaaatatct atcatgagat acaaatatta aagcaccttg aaagttttgt
     1381 aagtactctt ctaaaatatt aattgtagga atatctaaat cgtttgtagg ttcatccaaa
     1441 atcatacaat caactctttt tgtaaataga agtgctagtg caactctgtt tttctctcca
     1501 ccacttaaaa caccaacttt tttatctaaa tactctttt
//
LOCUS       DAOQAN010000341         1539 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_127285_length_1539_cov_1.861860, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000341 DAOQAN010000000
VERSION     DAOQAN010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127285_length_1539_cov_1.861860"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(26..883)
                     /locus_tag="PKO10_05725"
     CDS             complement(26..883)
                     /locus_tag="PKO10_05725"
                     /inference="COORDINATES: protein motif:HMM:NF023240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3333 domain-containing protein"
                     /protein_id="HNW66679.1"
                     /translation="MIKRKKKNKEHNPFYDPTLKKRHLSAKRFKKFTLTSLIFSVAFL
                     AFFLFDMIGKGIPAFNIAYIKVDVTFNEKTLEDTRFAVPTEYRNLVSRAALRDLPKLL
                     EENPTYMNSTQNIWILASSQVDQYIKNHNHNLKDKYIAKVDELYAQGLIDKKFNAIFF
                     ENGDSKIPEYAGIFSAVIGSILTLIITMAVAFPIGVMTAIYLEEFALDNKFTRFIEIN
                     INNLAAIPSILFGLLGLAIFINLFGMPRSSPLVGGLTLALMTLPIIIVSSRAALRQFQ
                     IVLDKLDMV"
     gene            complement(880..>1539)
                     /gene="pstC"
                     /locus_tag="PKO10_05730"
     CDS             complement(880..>1539)
                     /gene="pstC"
                     /locus_tag="PKO10_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HNW66680.1"
                     /translation="AIPIGLGSAIYMSEYASPKLRDYLKPILEILAGIPTVVYGFFAA
                     ITVAPLVVKVAAFFGLEATFNSALASGIVMGIMIIPLISSLSDDVIRAVPDSQRKAAF
                     ALGMTHGETIKNIVIPSAMPGIISASLLALSRALGETMIVVMAAGLRPNLSWNPLEDM
                     TTVTVTIVNSLVGDFEFNSPETLSAFALGLMLFIVTLILNMISLSMIRKFKEKYKVNT
                     L"
BASE COUNT          545 a          254 c          210 g          530 t
ORIGIN      
        1 ttagttactt gaattttatt taatcctaaa ccatatccag cttgtctaat actatctgga
       61 actgccttaa agctgctctt gaacttacaa ttataattgg taaagtcata agagcaagag
      121 ttaatccacc aaccaaaggt gaacttcttg gcattccaaa tagattaata aatattgcaa
      181 gtcctaaaag tccaaataaa attgaaggaa ttgctgcaag attatttatg tttatttcaa
      241 taaatcttgt aaatttatta tctagcgcaa actcttcaag gtaaatagct gtcattactc
      301 caattggaaa tgcaactgcc attgttataa ttaaagtcaa aatagaacct ataacagctg
      361 aaaagatacc agcatattca ggaatttttg aatcaccatt ctcaaagaat atagcattaa
      421 actttttatc tattaaacct tgagcataaa gttcatcaac ttttgctata tatttatctt
      481 ttagattatg attgtgattt tttatatatt ggtcaacttg actacttgct aaaatccaaa
      541 tattttgagt tgaattcata tatgttggat tttcttctaa taattttggt aaatccctaa
      601 gtgctgctct tgaaactaaa tttctatatt cagttggaac tgcaaatctt gtatcttcta
      661 aagttttttc attaaaagtt acatctactt ttatgtaagc tatattaaat gcaggaatac
      721 cttttcctat catatcaaaa aggaaaaatg ccaaaaatgc tactgaaaaa attaaagaag
      781 ttagagtaaa ctttttaaat ctttttgcac ttaaatgcct tttttttaga gttgggtcat
      841 aaaatggatt gtgttctttg tttttctttt ttctttttat cataatgtat tcactttata
      901 tttttcttta aattttctta tcattgacaa cgagatcata tttaaaatca aagttacgat
      961 aaacaacatc aatcctaaag caaaagcaga aagagtttca ggagagttaa attcaaaatc
     1021 tccaactaaa ctatttacta ttgtaactgt aaccgttgtc atatcttcaa gtggattcca
     1081 agagagattt ggtcttaaac ctgctgccat aacaacaatc attgtttctc ctaaagcacg
     1141 tgaaagtgca agaagtgacg cagagataat tccaggcatt gcacttggga tcactatatt
     1201 ttttatagtt tctccatgag tcattcctag agcaaatgct gcttttcttt gactatcagg
     1261 aacagctctt ataacgtcat cacttaaaga tgaaattaaa ggaataatca taattcccat
     1321 aacaattcct gatgctaaag cactgttaaa agtagcttca agtccaaaaa aagcagcaac
     1381 tttcacaaca agtggagcaa ctgtaatggc agcaaagaaa ccataaacaa cagttggaat
     1441 accagcaaga atttctaaaa taggttttag ataatctctt aatttaggac ttgcatattc
     1501 actcatataa atagcacttc caagaccaat aggaatagc
//
LOCUS       DAOQAN010000342         1536 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_127610_length_1536_cov_2.703579, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000342 DAOQAN010000000
VERSION     DAOQAN010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127610_length_1536_cov_2.703579"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="PKO10_05735"
     CDS             <1..184
                     /locus_tag="PKO10_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014469117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HNW66681.1"
                     /translation="GAQAVSYTTGVPAMIGSKLLYKGIWKNKGVFNIEEFDAKPFMDE
                     LMTQGLPWKILELDVK"
     gene            187..1335
                     /gene="nspC"
                     /locus_tag="PKO10_05740"
     CDS             187..1335
                     /gene="nspC"
                     /locus_tag="PKO10_05740"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014469116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0008216 - spermidine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HNW66682.1"
                     /translation="MNKNYEIIDSFEKLPSPAYVCEEELLEKNLKLLKRVQDETGVKI
                     LLALKGFAMYSTFDLCKKYLKGCCASGLHEALLAKEEFGGEVHTYSPAFKDEEIDEII
                     SISNHLVFNSFNQLKRYKDKAFKKVSLGVRLNPEYSSVEVDLYNPCAPNSRLGITKAN
                     FDESQLQYLEGFHFHALCEQNVDALQGALANFEKNFSQYFSQLKWVNFGGGHHITRAD
                     YDVEGLINLLKDFKSRYPHLEVYMEPGEAVGWKTGYLVATVLDIVNNGMDLAILDTSA
                     EAHMPDTLAMPYRAMIRNSAVAFEKKYTYRLGGNTCLAGDIIGDYSFDKPLKVGDKII
                     LEDMIHYTMVKTTTFNGIKLPSIVIKTKDNNYKIIKNFGYNDYKSRLS"
BASE COUNT          586 a          164 c          284 g          502 t
ORIGIN      
        1 tggagcacaa gcagtttctt atacaacagg agttccagct atgattggtt caaaactttt
       61 atataaaggt atttggaaaa acaaaggtgt atttaatata gaagagtttg atgcaaaacc
      121 atttatggat gagctaatga ctcaaggtct tccttggaaa atacttgaac ttgatgtgaa
      181 atagttatga ataaaaatta cgagattatt gatagttttg aaaagcttcc aagccctgca
      241 tatgtttgtg aagaggaact tttagaaaaa aatttaaaac ttttaaaaag agttcaagat
      301 gagacgggag ttaaaattct tttagcttta aaaggttttg ctatgtattc aacttttgat
      361 ttatgtaaaa aatatcttaa aggttgttgt gcttctggtc ttcatgaagc acttttagca
      421 aaagaggagt ttggaggaga agttcataca tatagtccag cttttaaaga tgaagagata
      481 gatgaaatta tatctatctc aaatcattta gtatttaact catttaatca actaaaaaga
      541 tataaagata aggcttttaa aaaagtatct ttaggagtta gattaaatcc tgagtattca
      601 agtgttgaag tagatttata taatccttgt gctccaaatt caagacttgg tattacaaaa
      661 gcaaattttg atgaatcaca actacaatat ctagaaggtt tccattttca cgcactttgc
      721 gaacaaaatg tagatgctct acagggtgct ttagcaaatt ttgaaaaaaa tttcagccaa
      781 tatttttctc aattaaaatg ggtaaatttt ggaggaggtc atcatattac aagagctgat
      841 tatgatgttg agggattgat taatctttta aaagatttta aatcaagata tcctcatctt
      901 gaagtttata tggaaccagg tgaagcagtt ggttggaaaa ctggttattt agtagcaact
      961 gtgcttgata ttgttaataa tggaatggat ttagcaattc ttgatactag tgcagaagct
     1021 catatgcctg atactttagc tatgccatat cgtgcaatga ttagaaatag tgcagtagca
     1081 tttgagaaaa aatatacata tagattaggt ggaaatactt gtttggctgg agatattatt
     1141 ggagattact cttttgataa acctttaaaa gtaggggata aaataatttt agaagatatg
     1201 attcattata ctatggttaa aactacaaca tttaacggta taaaattacc atcaattgtt
     1261 ataaaaacta aagataataa ttataaaatt ataaaaaact ttggatataa tgattacaaa
     1321 tcaagattat cttgataaaa aaattatcta ggagaaagta tggggagaga tagaagttta
     1381 ataagaaatg catttgaaga gggtgcagtt attgatacag aagaagatat aactcatggt
     1441 ttagataaaa ctagacctag aaaagagtta gaaaaaaaag ttgaaaatgg taaaacaaaa
     1501 gttcaaatat gggttaagaa agatacttta gagtat
//
LOCUS       DAOQAN010000343         1531 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_128285_length_1531_cov_2.394986, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000343 DAOQAN010000000
VERSION     DAOQAN010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128285_length_1531_cov_2.394986"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="PKO10_05745"
     CDS             complement(<1..599)
                     /locus_tag="PKO10_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66683.1"
                     /translation="MKSSFYIKLFGAFTLFILILLFLINIAFNSFYSMYSNKIEVKKI
                     EYILDTQALKFEDYIKNYSDKLLLIEPILSKMNNQNEIKSFVNDSLFKDLNILTFRVV
                     DFDSKEILKLVNRNKQDNYFSQRLKSIFMEPYFKELQSLNKFDIFSYCEDEENNFLNF
                     AIRGENKFYILKVDLKTILDEISNSYNKKILIKDINGNF"
     gene            complement(596..>1055)
                     /locus_tag="PKO10_05750"
     CDS             complement(596..>1055)
                     /locus_tag="PKO10_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66684.1"
                     /translation="FSSRQNMSSNLFDIVKSISNKFDNSLLEIIYDFDENIKINFAHN
                     DLKNIIINILSTVVEFAKKYSLERVIVNISLQIEENSDEIVLKIEDNIKDIRKKEFFE
                     QFLNFEDENKFDSKIYLAKLLADKNQAIFLCNILENSTSYYIKFKKAKIF"
     assembly_gap    1056..1065
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1066..>1531)
                     /locus_tag="PKO10_05755"
     CDS             complement(<1066..>1531)
                     /locus_tag="PKO10_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNW66685.1"
                     /translation="DMSQNFYKNLWKELRAKRIWQGEVKNIDKFGNTYWIKTSLFPKY
                     NDKKQHIGYSSIRTDITATKQLEKTNRLLKEDLSNRLNDLKIQDETALNSLKVALMSK
                     ILDSFSHQWKTPISKIYFELLKLENIKKDLDILKIEDVKNNIESELRELSDM"
BASE COUNT          542 a          197 c          120 g          662 t
ORIGIN      
        1 aagaaattgc catttatatc tttaattaat atttttttat tataagagtt tgaaatttca
       61 tctaaaatag tttttaaatc aacctttaag atataaaact tattttcacc tctaattgca
      121 aaatttaaaa aattattctc ttcatcttca caataactaa aaatgtcaaa cttatttaaa
      181 gattgcaact ctttaaaata tggctccata aatatagatt ttaatctttg agaaaagtag
      241 ttatcctgtt tatttctatt aactaacttt aaaatctctt ttgaatcaaa atcaacaact
      301 ctaaaagtta aaatattcaa atctttaaat aaactatcat ttacaaaact ctttatctca
      361 ttttgattat tcatttttga taaaattggc tctattaata gaagtttatc actgtaattt
      421 tttatgtaat cttcaaattt tagagcttgt gtatctaaaa tatactcaat tttttttacc
      481 tcaattttat tactatacat tgaataaaaa ctattaaaag caatatttat aagaaataga
      541 agtattaaaa taaaaagagt aaatgcacca aaaagtttta tataaaatga actcttcaaa
      601 atatttttgc ctttttaaat tttatataat aacttgtact attttctaat atattacata
      661 aaaatatagc ttgattttta tctgccaaaa gttttgctaa atatattttt gaatcaaact
      721 tattctcatc ttcaaaattc aaaaattgtt caaaaaactc tttttttctt atatctttta
      781 tattatcttc tatttttaaa actatttcat ctgaattttc ttctatttgc agtgaaatat
      841 ttacaataac tctctctaaa gagtattttt ttgcaaactc tacgacagta gaaagaatat
      901 ttataataat atttttcaaa tcattatgtg caaaattaat tttaatattt tcatcaaaat
      961 catatattat ctcaagtaat gaattatcaa atttatttga tatacttttt acaatatcaa
     1021 atagatttga actcatattt tgtcttgaag aaaatnnnnn nnnnnaacat atctgataac
     1081 tctcttaact cactctctat attatttttt acatcctcta tttttaagat gtctaaatct
     1141 tttttaatat tttctagttt taataactca aaatatattt ttgatattgg tgttttccat
     1201 tggtgtgaaa agctatccaa aatttttgac ataagtgcaa cctttaaaga gttcaaagct
     1261 gtttcatctt ggatttttaa atcatttaat ctatttgata aatcttcttt taaaagtcta
     1321 tttgtttttt caagttgttt tgttgcagtt atatctgttc taatagatga atatccaata
     1381 tgctgctttt tatcattata ttttggaaat aaagatgttt taatccaata tgtatttcca
     1441 aatttatcta tattttttac ttcaccttgc catattcttt ttgctcttaa ctctttccaa
     1501 agatttttat aaaagttttg tgacatatca g
//
LOCUS       DAOQAN010000344         1526 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_128901_length_1526_cov_3.104691, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000344 DAOQAN010000000
VERSION     DAOQAN010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128901_length_1526_cov_3.104691"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..720)
                     /gene="lgt"
                     /locus_tag="PKO10_05760"
     CDS             complement(<1..720)
                     /gene="lgt"
                     /locus_tag="PKO10_05760"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005023581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNW66686.1"
                     /translation="MEFWQNIYSHFNPVAFSIGDIAVHWYGIMYALALLSAIFIAKWF
                     IKYDNINISQDIFDSYIWWAEIGVILGARLGYILFYDSNTTYYLTNPWQIFNPYVNGT
                     YVGIAGMSYHGAFIGFIIASILFCKRKNINFWFITDIAVLGVSAAYVFGRIGNFFNQE
                     LVGRVTDVSWGIYVDGILRHPSQLYEAFLEGIVVFLILVYFRNRKKFDGQLALMYGIF
                     YTIARIIAEFYREPDPQIGFIA"
     gene            complement(724..>1526)
                     /locus_tag="PKO10_05765"
     CDS             complement(724..>1526)
                     /locus_tag="PKO10_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNW66687.1"
                     /translation="KSVNLIISRKNIDDIKKNRVAIFIGCMSNYTYTNTGDSLVKILK
                     KLELDIFIPKKQLCCGAPAYFTGAFDTVDYLTKKNIEYFESWIDSVDAVIIPEATCSA
                     MINKDWEHYLHDQPEWKERAIKLSNKIFLATKWLENNTKLKELLAKSGKKFDQMVTYH
                     DPCHAKKMQNVWKEPRELLKQNYVLKEMSDSNRCCGFGGVTMQTEKYEFSKAAGAPKA
                     AMIRDSKAQIVSAECSACRMQITNSLYLADVDVVFKNPIELIAEALED"
BASE COUNT          519 a          280 c          177 g          550 t
ORIGIN      
        1 agctataaat ccaatttgag gatcgggctc tctataaaac tctgcaatta ttcttgcaat
       61 tgtataaaaa ataccgtaca ttaaagctaa ttgtccatcg aatttttttc tatttctaaa
      121 gtaaacaagt attaaaaata ctacaattcc ctctaaaaat gcctcgtata gttgagaagg
      181 gtgtcttaaa attccatcaa cataaattcc ccagctaaca tctgtaactc ttcctacaag
      241 ctcttgatta aagaagtttc caattcttcc aaaaacataa gcagcactaa cacctaaaac
      301 tgcaatatca gttataaacc aaaaattaat attttttctt ttgcaaaata gaattgaagc
      361 aataataaat ccaataaaag ctccatgata actcattcca gctattccaa cataagttcc
      421 atttacataa ggattaaaga tttgccaagg atttgttaaa tagtaagttg tatttgaatc
      481 ataaaaaaga atatatccta gtcttgctcc taaaataact ccaatttcag cccaccagat
      541 atatgagtca aaaatatctt gacttatatt gatattgtca tatttgataa accattttgc
      601 aataaaaata gcacttaaaa gtgccaatgc gtacataatt ccataccaat gaactgcaat
      661 atcaccaata ctaaaagcta ctggattaaa atgagagtat atattttgcc aaaattccat
      721 tttttaatcc tctaaagctt cagctattaa ttctataggg tttttaaata caacatcaac
      781 atcagctaga taaagagaat ttgttatttg cattctacaa gcactacatt cagcacttac
      841 aatttgagct ttgctatctc ttatcatagc agcttttgga gctccagcag cttttgaaaa
      901 ctcatatttt tctgtttgca tagtaactcc accaaatcca caacatctat ttgaatcact
      961 catctctttt aaaacatagt tttgttttag aagttctctt ggttccttcc aaacattttg
     1021 catctttttt gcatggcaag ggtcatgata agttaccatt tgatcaaatt ttttaccgct
     1081 ttttgctaat aattctttta attttgtatt gttttcaagc cattttgttg ctaaaaatat
     1141 cttatttgat agtttaatag ctctctcttt ccattctggt tggtcatgta agtagtgctc
     1201 ccagtcttta tttatcattg cactacaagt agcttctgga ataataacag catcaacgct
     1261 atctatccaa gattcaaaat attcaatatt ttttttagtt aaataatcaa ctgtatcaaa
     1321 agctcctgta aaataggcag gtgccccaca acataactgt ttttttggaa taaaaatatc
     1381 aagctctaat ttttttaata tttttactaa actatctcct gtatttgtgt atgtgtagtt
     1441 actcatacat ccaataaata tagcaactct gtttttctta atatcatcaa tatttttcct
     1501 ggaaataatc aagttgacgc ttttat
//
LOCUS       DAOQAN010000345         1526 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_128919_length_1526_cov_2.740993, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000345 DAOQAN010000000
VERSION     DAOQAN010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128919_length_1526_cov_2.740993"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..107)
                     /locus_tag="PKO10_05770"
     CDS             complement(<1..107)
                     /locus_tag="PKO10_05770"
                     /inference="COORDINATES: protein motif:HMM:NF013522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HNW66688.1"
                     /translation="MPVINVKMTHEDGGATVEQKEELVKGINELFSKIF"
     gene            complement(150..941)
                     /gene="trpC"
                     /locus_tag="PKO10_05775"
     CDS             complement(150..941)
                     /gene="trpC"
                     /locus_tag="PKO10_05775"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HNW66689.1"
                     /translation="MILEKINNKTLEDVKKREEKISLDELENIIADGNFPTKDVKKFL
                     KSSIDEPIRIIAEVKKASPSKGVIKEDFDHLQIAKEYNDFGANAISILTEPHFFLGNL
                     DYLKEIKNITNIPLLRKDFILTKYQIAEALIYGADFILLIAKSLSQDELNELYSYARG
                     LNLEVLVEIHDFEDLTKALKSKADIIGINHRNLKTFEMDMNLCEELIPLIPEGKIIVA
                     ESGVSDIEVIKRLHNLGADAFLIGEHFMRVPSIKEELKKFKNALN"
     gene            complement(919..>1526)
                     /locus_tag="PKO10_05780"
     CDS             complement(919..>1526)
                     /locus_tag="PKO10_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66690.1"
                     /translation="EMFLYYKNSEDALQLNKTANLMFQNFEANDIIIFLEGNNLEDDF
                     LLELYKRTKQPEKAYNLLKKLYTNSSNPDYLAQQAIIEFELAEDKNSVLASVIEKFNI
                     SLKTSSNPIYQNYLAYLLIDYDIDIPKGLVLVKKALEQDPTNIAFIDTLAWGDYKSKN
                     CKDAYKNMKLIVDEIGLNDEEIKMHWEKIKECKENDTRKNK"
BASE COUNT          490 a          253 c          138 g          645 t
ORIGIN      
        1 ttaaatattt tactaaaaag ttcatttata ccttttacta actcctcttt ttgctcaact
       61 gttgctccgc catcctcatg cgtcattttt acatttatta ctggcatttt tactcctttt
      121 ttaaattaaa tttaataata taaaatagat taatttagag catttttaaa ttttttcaat
      181 tcctctttta tacttggaac tctcataaaa tgctctccta ttaaaaaagc atcagctcca
      241 agattatgga gtctttttat aacttcaata tcactaactc cagattcagc aacaattatt
      301 ttaccttcag gaataagtgg aattagctcc tcacataaat tcatatccat ctcaaaagtt
      361 tttaaatttc tatggtttat tcctataata tctgctttac tttttaaagc tttagtcaaa
      421 tcctcaaaat cgtgaatttc aactaaaact tctaaattca aacctctagc ataactatat
      481 aattcattta actcatcttg acttagtgat tttgcaatta atagtataaa atctgctcca
      541 tatatcaaag cctctgcaat ttgatatttt gtaagaataa aatcttttct taaaagtgga
      601 atatttgtaa tattttttat ctcttttaaa taatccaaat tccccaagaa aaagtgtggt
      661 tctgttaata tagaaattgc atttgcacca aaatcattgt actcttttgc tatttgaaga
      721 tgatcaaaat cttcttttat aaccccttta ctaggacttg ctttttttac ttcagcaatt
      781 attcgaatag gttcatcgat tgaagatttt aaaaatttct ttacatcttt tgtggggaaa
      841 ttaccatctg ctattatatt ttcaagctca tctaaagata ttttctcttc tctttttttt
      901 acatcttcta aagttttatt atttattttt tctagtatca ttctctttac actccttaat
      961 tttttcccaa tgcatcttta tctcttcatc atttaaacct atttcatcta caatcaattt
     1021 catattttta tatgcatctt tacaattttt actcttataa tcgccccatg ctagagtatc
     1081 tataaaagca atatttgtag gatcttgctc caatgctttt tttaccaaaa ccaaaccttt
     1141 tggtatatca atatcataat caattaataa atatgccaaa taattttgat aaataggatt
     1201 tgaagatgtt tttaaagaga tattaaattt ttcaataaca cttgctaaaa cactattttt
     1261 atcttctgct aattcaaatt caataatagc ttgctgtgct aaataatcag gatttgaact
     1321 atttgtatat aattttttga gtaaattata tgctttttct ggctgttttg ttcgtttata
     1381 aagctctaaa agaaaatcat cttcaagatt atttccttct aaaaatatta taatatcatt
     1441 tgcttcaaaa ttttgaaaca ttaaatttgc tgttttattt aattgtaaag catcttcact
     1501 atttttatag tataaaaaca tctctt
//
LOCUS       DAOQAN010000346         1522 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_129437_length_1522_cov_3.447171, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000346 DAOQAN010000000
VERSION     DAOQAN010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129437_length_1522_cov_3.447171"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            162..596
                     /locus_tag="PKO10_05785"
     CDS             162..596
                     /locus_tag="PKO10_05785"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HNW66691.1"
                     /translation="MFKKSNFFLTILILILGLALNSFSSTKGLETASNNQVLKNTKWE
                     LKSLNKKDIKKVEKVAIINFEKENKVFGNLGCNSFFGKVDIKPNNINISKVGSTMMMC
                     SDMSVERDYMEVLNTVTTYKIDGNSLIFFDKNNKEIARFIKR"
     gene            complement(626..1444)
                     /gene="panC"
                     /locus_tag="PKO10_05790"
     CDS             complement(626..1444)
                     /gene="panC"
                     /locus_tag="PKO10_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012139951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HNW66692.1"
                     /translation="MQIVKTIEELQNIRKNLNGNIGFVPTMGALHDGHISLIRKAKDE
                     NEVVIVSIFVNPTQFLKGEDLNKYPRKEEADIKICQMCKVDYLFIPQINSMYEKDEVL
                     IKAPINSYVLEGFTRPGHFDGVLQVVLKLFNLTQPKNAYFGKKDAQQLALIQQMVKNL
                     FLPINIIACDIVRESSGLALSSRNVYLTPEQKEEALKISKSIYMAGNLIAKGERDSKI
                     VKDKIYEILENLDVEYVRVVNKRFDEIEKIEPSNTIILVVVRFGNVRLLDNIWM"
BASE COUNT          544 a          198 c          171 g          609 t
ORIGIN      
        1 ccatatgtag ttttttcttt ggtttttctt cactaaattt catctaatat tatccatcat
       61 ttataaattt ttgaaagatt ttagctaact tttgcattta tataaattaa taagtacaaa
      121 aatagtatta taaaactcta aataaaattt aggagaaaat tatgtttaag aaatcaaatt
      181 tctttttaac tattttgata cttattttag gattagcttt aaactctttt agttcaacta
      241 aaggattaga gacagcaagc aataatcaag tcttaaaaaa tacaaagtgg gaacttaaaa
      301 gcttaaataa aaaagatata aaaaaagttg aaaaagttgc tattataaat tttgaaaaag
      361 agaataaagt ttttggaaat ttaggttgta atagcttttt tggaaaagtt gacataaaac
      421 caaacaatat taatatctct aaagttggta gcactatgat gatgtgttct gatatgagtg
      481 tagaacgtga ttatatggaa gttttaaata cagtaacaac ttataaaata gatggaaaca
      541 gtttaatatt ttttgataag aacaataaag agatagcaag atttataaaa agatagttta
      601 aatactatct ttttatctta ttaatttaca tccaaatatt atcaagaagt cttacatttc
      661 caaatctaac aacaactaaa attattgtgt ttgatggttc tattttttct atttcatcaa
      721 atcttttatt tacaactctc acatactcta catcaagatt ttctaaaatt tcatagattt
      781 tatcttttac tatttttgaa tctcgctccc cttttgctat tagatttcca gccatataaa
      841 tagattttga aattttaaga gcttcctctt tttgttcagg agttaaatat acatttctag
      901 aactaagagc taatccgcta ctttctctta caatatcaca agcgataata tttataggta
      961 aaaatagatt ttttaccatt tgttgtataa gtgctagttg ttgggcatct tttttaccaa
     1021 agtaagcatt ttttggttgt gttaaattaa acaattttaa aacaacttgc aatactccat
     1081 caaaatgtcc tggtcttgta aatccttcaa gtacataact attaatagga gcttttatta
     1141 aaacttcatc tttttcatac atactgttta tctggggtat aaaaagataa tcaactttac
     1201 acatttgaca aattttaata tctgcttcct cttttcttgg atatttattt aaatcttcac
     1261 cttttaaaaa ttgtgtagga tttacaaaaa tagatactat tacaacttca ttttcatctt
     1321 ttgcttttct gattagtgaa atatgtccat catgtaaagc tcccattgtt ggaacaaatc
     1381 caatatttcc attcaaattt tttctaatat tttgtaactc ttctatagtt ttaactattt
     1441 gcaatatata ttcctttaaa agttttaatt atttttcatt gtagtttgga tttaatttta
     1501 ataaaactaa atctgcaaac at
//
LOCUS       DAOQAN010000347         1517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_130156_length_1517_cov_2.985636, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000347 DAOQAN010000000
VERSION     DAOQAN010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130156_length_1517_cov_2.985636"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="PKO10_05795"
     CDS             <1..369
                     /locus_tag="PKO10_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HNW66693.1"
                     /translation="GLKNAGLTDYLAIILKDLALRGETIAVLGTGFIAAFLSAIMNNM
                     PTIMIMDIALNDIQNQAMIYANIVGCNLGPKMTPFGSLATLLWLHVLAKKGVKISFAQ
                     YSKFGLIITPPVLFIVLLSL"
     gene            448..918
                     /locus_tag="PKO10_05800"
     CDS             448..918
                     /locus_tag="PKO10_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNW66694.1"
                     /translation="MCNNTLLGQRLNLSSSSDIFDNLIKKNEWETLIRLFSLNILDIN
                     HRDCKGRNILYFAILNKKYEYIKILFDLRVSSRVNFSLNAINFAVCLDDIKSLEILLK
                     CGLSVDTLDEIGTSPLIYAILYNKKKSAEFLIKSGADLELEDFMGNCAKKMLQN"
     gene            complement(935..>1517)
                     /locus_tag="PKO10_05805"
     CDS             complement(935..>1517)
                     /locus_tag="PKO10_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein-PII uridylyltransferase"
                     /protein_id="HNW66695.1"
                     /translation="SSNQIFLRLKSKDILNIAIKAKDVQNYSFDIINDDFLKIRIIRK
                     IPLNLGYLLGRLQYMDMNSMNIFKLYDEKKNFEICFSKKALDEELYLIEEIIHESFDM
                     SKKIRINKPIIKKDDIKIDFNHSEYLASMKINTKDQKGLFAYIAKVFDDFNIEVESAK
                     LITSKGYAKDLILIEKNDNFYKNIKKILDLICV"
BASE COUNT          542 a          167 c          196 g          612 t
ORIGIN      
        1 ggtctaaaaa atgcaggact tacagattat ttagcaataa ttttaaaaga tttggctctt
       61 agaggtgaga caattgcagt tttaggaaca ggttttatag ccgcattttt aagtgctatt
      121 atgaataata tgccaacaat tatgattatg gatatagctt taaatgatat acaaaatcaa
      181 gctatgattt atgcaaatat tgttggatgt aatcttggac caaaaatgac accatttggt
      241 agccttgcaa ctctattatg gctacatgtt ttagcaaaaa aaggtgtgaa aatttcattt
      301 gcacaataca gtaaatttgg tttgatcatc acaccacctg ttttatttat tgtgttgttg
      361 agtttgtagg ataattatcc ttttttaaat aaactttaaa gttttatata ttatgatatt
      421 cattatcatt ttaaaaaagg aaatattttg tgtaacaata ctcttttagg gcaaaggcta
      481 aatttaagta gttctagtga tatttttgat aatttaatta aaaaaaatga gtgggaaact
      541 ctgattagat tatttagttt aaatatcttg gatataaatc atagagattg caaaggtaga
      601 aatattttat attttgcaat attaaataaa aaatatgagt atataaaaat actttttgat
      661 ttaagagtta gttctagagt aaatttttct ctaaatgcaa tcaattttgc tgtttgttta
      721 gatgatataa aatctttaga gatactttta aaatgtggtt taagtgtaga tactttagat
      781 gagataggaa cttcaccttt aatatatgca attttatata ataaaaaaaa gagtgcagaa
      841 tttttgatta aatctggtgc tgatcttgag ttagaagatt ttatgggaaa ttgtgctaaa
      901 aaaatgttac aaaattaata gaaaatagta gattttaaac acaaattaga tctaatattt
      961 tctttatatt tttatagaag ttatcatttt tttctattaa aatcagatcc ttcgcatatc
     1021 cttttgaagt aataagtttt gcactttcaa cttctatgtt aaaatcatca aaaacttttg
     1081 caatatatgc aaaaagtcct ttttgatctt ttgtatttat cttcatagat gctaagtatt
     1141 cactatgatt aaaatctatt tttatgtcat ctttttttat aattggttta tttattctta
     1201 tttttttact catatcaaaa gattcatgaa taatctcttc gattaaataa agctcttcat
     1261 ctaaagcttt tttagaaaaa catatttcaa aattcttttt ctcatcatat agtttgaaaa
     1321 tattcatact attcatatcc atatattgaa gtcttcccaa caaatatccc aaatttaaag
     1381 gaatttttct aataatccta attttgagaa aatcatcatt tataatatca aaactatagt
     1441 tttgtacatc ctttgctttt attgcaatat ttaaaatatc tttacttttt agtcttaaaa
     1501 atatctgatt tgaagag
//
LOCUS       DAOQAN010000348         1515 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_130480_length_1515_cov_2.662329, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000348 DAOQAN010000000
VERSION     DAOQAN010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130480_length_1515_cov_2.662329"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /gene="metX"
                     /locus_tag="PKO10_05810"
     CDS             complement(<1..707)
                     /gene="metX"
                     /locus_tag="PKO10_05810"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004414 - homoserine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HNW66696.1"
                     /translation="MLEIETKKEKFNEPLYLESGRLLEEFEIVYETYGRLNEDKSNVI
                     VICHALSGSHHAAGRYENEAKAGWWDKFIGDKKAIDTEKYFVICSNNIGSSYGSTSPL
                     SINPSTKKEYRLKFPVLTISDIVNAQMRLYKKLGIKNAVAVIGGSMGGMQALCYAIEH
                     PTFAKHYIPMATTAYTRPWAIALNKIAIEAIRHDPNFQNGNYEKDDLKARGLVGLAVG
                     RMAGLIAYLSPNLFINK"
     gene            874..1134
                     /locus_tag="PKO10_05815"
     CDS             874..1134
                     /locus_tag="PKO10_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66697.1"
                     /translation="MNKIEELKKLLEKEVLPELKAEIEALEKLLSKKNDKEIKEELNY
                     FLEVKKFYDEALKLINENKLTQEEAENILLDLEDMEDDEEEI"
     gene            1134..>1515
                     /locus_tag="PKO10_05820"
     CDS             1134..>1515
                     /locus_tag="PKO10_05820"
                     /inference="COORDINATES: protein motif:HMM:NF016064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HNW66698.1"
                     /translation="MFDLEIYEIVFIAFFAIAFVFGFFAQQKQFCFSGSLKDYIQIKS
                     TKRAASVVMAMIVAIISTQIFASIFDIDLSSSSYFKSNINYFAIIIGGALFGAGMMIA
                     DGCSSRSIVKFSQGDANALVTLLFI"
BASE COUNT          522 a          199 c          215 g          579 t
ORIGIN      
        1 aatttgttta taaatagatt tggacttaaa tatgcaatta atccagccat tcttccaact
       61 gcaagaccaa ctaaccctct tgcttttaaa tcatcttttt catagtttcc attttgaaaa
      121 tttggatcat gtcttattgc ttcaatagct attttattta aagctattgc ccaaggtctt
      181 gtatatgctg ttgttgccat tggaatatag tgttttgcaa aagttggatg ctcaattgca
      241 taacaaagag cttgcattcc acccatactt cctccaataa ccgcaactgc atttttaatt
      301 cccaattttt tataaagtct catttgagca tttacaatat ctgaaattgt taaaacagga
      361 aattttaatc tatactcttt ttttgtagat ggattaatac ttaaaggact tgtggaacca
      421 tatgaacttc caatattatt tgaacaaatt acaaaatatt tttctgtatc aatagctttt
      481 ttatctccta taaacttatc ccaccaacca gcttttgcct cattttcata acgaccagct
      541 gcgtgatgac ttccagatag agcatgacaa ataactataa catttgattt atcttcattt
      601 aatcttccat aagtttcata aactatctca aactcttcaa gaagccttcc actttcaaga
      661 taaagtggtt cgttaaattt ctcttttttt gtctctattt ctaaaatata ttccaaattt
      721 ttctattttt ttagattaaa gtctatctaa actaaaataa aaaagccttt tagtgacata
      781 tttttaaaaa aattaaagat ataaaacttt ttaatcttat attataagta attttttata
      841 taatcatagt ttaaaattat aaaaaggatt tttgtgaata aaattgaaga gttaaaaaaa
      901 cttttagaaa aagaggtttt accagaactt aaagctgaaa tagaggcttt agaaaagtta
      961 ctttcaaaga aaaatgataa agagataaaa gaggaattaa actacttttt agaggtaaaa
     1021 aagttttatg atgaagcttt gaaacttata aatgaaaata aattaactca agaagaggcg
     1081 gaaaatattt tactagattt agaggatatg gaagatgatg aggaggaaat ttagtgtttg
     1141 atttagagat ttatgaaatt gtttttatag cgttttttgc tattgctttt gtatttggat
     1201 tttttgctca acaaaaacag ttctgtttta gtggaagttt aaaagattat atacaaatta
     1261 aatcaacaaa aagagcagct agtgttgtta tggctatgat tgtagctatt atttctactc
     1321 aaatatttgc atctattttt gatattgatt taagttctag tagctatttt aaaagtaata
     1381 taaactactt tgctattatt attggtggtg ctttatttgg tgctggaatg atgatagctg
     1441 atggttgtag tagtagaagc atagtaaaat tttctcaagg tgatgcaaat gctttagtaa
     1501 cgctactttt tattg
//
LOCUS       DAOQAN010000349         1506 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_131775_length_1506_cov_2.501034, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000349 DAOQAN010000000
VERSION     DAOQAN010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131775_length_1506_cov_2.501034"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1039)
                     /locus_tag="PKO10_05825"
     CDS             complement(<1..1039)
                     /locus_tag="PKO10_05825"
                     /inference="COORDINATES: protein motif:HMM:NF024818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNW66699.1"
                     /translation="MSNHAKNKFDIICNTVDNGIIVLNKDLKVFFWNRWLETRTGIEA
                     NSIIDKNILDFYSNIDEKKLKRKIITALKLNSPTFYTPQTDDFLINIEINNISDRVFN
                     QMQQSITITPLDLEEGLVILYIYDITFLSEINYKLEIAKKSLSEKNEELRLILDTTME
                     AIIIFKDNSVVDCNKIAIELFNKQMKYELINKSFNDLIHNKNRDILNEKEPFETNLIR
                     EDGSEFNAIINIKDTSLNNQIFKIVTIVDIEDLKRKENLMAEQTKLAAMGEMLGNIAH
                     QWRQPLNIISMSSSNLKLKNDIGELCSSTLSESLSLILRTTNHLSDTIDTFNDFLKTD
                     KEKSFFNVNENI"
     gene            complement(1036..>1506)
                     /locus_tag="PKO10_05830"
     CDS             complement(1036..>1506)
                     /locus_tag="PKO10_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014474062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HNW66700.1"
                     /translation="AVGLKDYLAKELNFKDEHFVASQQINGPLSGENMFIIDKKSATN
                     MSIKFSFSDDQISNEDISDITLEITNILSSSTISKLAENMETSVSFSAPTIKTINSNN
                     QLNNIFISNYEKVIIISTKLEFDDLNIHGELFILTTDNSILFIKDKLNKILDEL"
BASE COUNT          505 a          199 c          147 g          655 t
ORIGIN      
        1 tgatattttc atttacatta aaaaagcttt tctctttatc tgtttttaaa aaatcattaa
       61 atgtgtcgat agtatcagac aaatgatttg ttgttcttaa aataagagat aatgactcac
      121 ttaatgtaga actgcataac tctccaatat cattttttaa tttaagattt gaagatgaca
      181 tagagataat atttaaaggt tgtctccatt gatgagcaat atttccaagc atttcaccca
      241 tagcagctag ttttgtctgt tctgccataa gattctcttt tctttttaaa tcttctatat
      301 ctacaatagt tacaatttta aatatttggt tgtttaaact tgtatcttta atgtttataa
      361 tagcattaaa ttcacttcca tcttctctta ttaaattagt ttcaaatggc tctttttcat
      421 ttagaatatc tctgttttta ttatgtatta aatcattaaa acttttattt attaactcat
      481 atttcatctg tttattaaat agttcaattg ctattttatt acaatcaaca acactattat
      541 ctttaaaaat aattatagct tccatagtag tatctaaaat aagtcttaat tcttcattct
      601 tttcacttaa actttttttt gcaatttcaa gtttatagtt tatttcactt aaaaaagtta
      661 tatcgtatat atataaaata actaaacctt cttccaagtc taatggagtt atagtaatgc
      721 tttgttgcat ttgattaaat actctatcag agatgttatt tatctcaata tttattaaaa
      781 aatcatctgt ttgtggagta taaaatgttg gagagtttag ttttaaagct gttattatct
      841 ttctttttaa ttttttttca tcaatatttg aataaaaatc taaaatattt ttgtcaataa
      901 tactatttgc ttctatgcca gttctagtct ctaaccatct attccagaaa aatactttta
      961 aatctttgtt taatacaata atcccattat ctacagtatt acaaataata tcaaatttat
     1021 tttttgcatg attactcata gttcatccaa tatcttattt aatttatctt ttataaataa
     1081 aattgaattg tcagttgtta aaataaatag ttcaccatga atatttaagt catcaaactc
     1141 caattttgta gatattatta taactttttc atagttactt ataaatatat tgtttaattg
     1201 attgtttgaa tttatagttt ttattgttgg agcagaaaaa gagacgctag tttccatatt
     1261 ttctgctaat ttacttatag ttgaagatga taaaatattt gtaatttcta aagttatatc
     1321 gctaatatct tcatttgaaa tttgatcatc actaaaacta aatttaatag acatatttgt
     1381 agcagatttt ttatctatta taaacatatt ttcaccactt aaaggaccat ttatttgctg
     1441 tgatgctaca aagtgttcat ctttaaaatt taactctttt gctaaataat cttttaaacc
     1501 tacagc
//
LOCUS       DAOQAN010000350         1498 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_132856_length_1498_cov_2.851005, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000350 DAOQAN010000000
VERSION     DAOQAN010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1498
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132856_length_1498_cov_2.851005"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..839)
                     /locus_tag="PKO10_05835"
     CDS             complement(<1..839)
                     /locus_tag="PKO10_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002953065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNW66701.1"
                     /translation="MKINYCDALVIGGGLAGLRAAVAAQKKGLSTIVLSLVPVKRSHS
                     AAAQGGMQASLGNSKMSDGDNEDLHFADTVKGSDWGCDQDVARMFVHTAPKAIRELAS
                     WGVPWSRVEKGTREAVINAKKTTITEDEDRHGLITSRDFGGTKKWRTCYTADATGHTM
                     LFGVANEALKHNVDIRDRKEAISLIHEDGRCYGAVVRDLITGELEAYVAKGTCIATGG
                     YGRVFKQTTNAVICEGTGAAIALETGIATLGNMEAVQFHPTPIVPSGILLTEGCRGDG
                     GIL"
     gene            complement(839..>1498)
                     /locus_tag="PKO10_05840"
     CDS             complement(839..>1498)
                     /locus_tag="PKO10_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase cytochrome b subunit"
                     /protein_id="HNW66702.1"
                     /translation="SILVSNDFMYQVTKLLEGSFIFEDGNPLLVSIAALVIFVIFIVH
                     AALGMRKLPGNFKQYQVIKAHSKSMGHDDTKLWFTQAFTGFAMFFLGSVHLYVIMTHP
                     DQIGPYESSARVWDEYMWPLYILLLLAVEFHGTIGLYRLCVKWGWFDGENPKATRKAL
                     KKLKWALTVFFLVLGFASLAAYMKIGYENSKNNIPRDSFKPSAHIMNYDLKTKSVGGI
                     A"
BASE COUNT          473 a          317 c          198 g          510 t
ORIGIN      
        1 cttagaattc caccatctcc tctacaacct tctgttaata aaataccaga aggaacgatt
       61 ggtgttgggt ggaactgaac agcttccata tttcctaaag ttgcaattcc agtttcaaga
      121 gcaattgctg cacctgtacc ttcacaaatt acagcatttg tagtttgttt aaatactctt
      181 ccatatccac ctgttgcaat acaagtacct tttgcaacat aagcttctaa ttcacctgta
      241 attaaatctc taacaacagc tccataacat cttccatctt catgaattaa actaattgct
      301 tcttttctat ctctaatatc aacattatgt tttaaagctt catttgcaac accaaataac
      361 attgtgtgtc cagttgcatc agctgtataa catgttctcc attttttagt accaccaaaa
      421 tctcttgaag tgataagtcc gtgtctatct tcatcttctg ttatagttgt ttttttagca
      481 ttaataacag cttctctagt acctttttca actcttgacc aaggaacccc ccagcttgct
      541 aactctctaa tagcttttgg tgcagtgtgt acaaacattc ttgcaacgtc ttggtcacat
      601 ccccagtcac taccttttac agtatctgca aagtgtaaat cttcattatc tccatcagac
      661 attttagagt ttcctaaaga tgcttgcata ccaccttgag cagcagcact atgagatctt
      721 ttaactggaa ctaaagataa aacaattgtg cttaatccct ttttttgtgc agcaacagca
      781 gctctaagac ctgcaagtcc tccacctatt actaatgcat cacagtaatt aattttcatt
      841 atgcgattcc tccaacactt ttagttttta agtcataatt cattatatgt gcagatggtt
      901 taaatgaatc tctaggaata ttatttttag agttttcgta accaattttc atataagcag
      961 caagtgatgc aaatcctaaa actaagaaaa atactgttaa cgcccattta agttttttaa
     1021 gtgcttttct tgttgctttt ggattttcac catcaaacca tccccatttt acacaaagtc
     1081 tataaagacc aattgttcca tggaattcaa cagctaatag taaaagaata tatagtggcc
     1141 acatatattc atcccaaact ctagcactac tttcgtaagg acctatttga tcagggtgag
     1201 tcataattac atataaatgc actgatccta ggaagaacat tgcaaatcct gtaaatgcct
     1261 gtgtaaacca taatttcgta tcatcatgtc ccatactttt tgaatgagcc ttaattactt
     1321 ggtactgttt aaagttacct ggtaattttc tcattcctaa agcagcatga acaataaaaa
     1381 taacaaaaat aacaagagcg gcaatagata ctaaaagtgg atttccatct tcaaatataa
     1441 agctaccttc taataatttt gtaacttggt acataaaatc atttgacacc aatattga
//
LOCUS       DAOQAN010000351         1497 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_133000_length_1497_cov_2.703190, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000351 DAOQAN010000000
VERSION     DAOQAN010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1497
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133000_length_1497_cov_2.703190"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="PKO10_05845"
     CDS             <1..453
                     /locus_tag="PKO10_05845"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNW66703.1"
                     /translation="IKIVFIKTKDNLIPIISTNTNLSDIEIINTYKKRWNIEQGYKDL
                     REYFQFGKEENRIYEALIARITLSFLAYNITSYINRINNEPQTLGNLFRDLECQLETL
                     AISMELFLKILEQLIQSAQIVKRNKDLEQIIEILRVHTKKQLGFMCES"
     gene            complement(480..>1497)
                     /locus_tag="PKO10_05850"
     CDS             complement(480..>1497)
                     /locus_tag="PKO10_05850"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNW66704.1"
                     /translation="DPDVRYDMNQAEVTNIVNSWNNVKFQNSIDYTIVWILTFVVFFS
                     IIAFIHRTVTLNILNKKLKYTVEEKTKELKYLNENLQLAIDKKTKELLEKEAILNQKA
                     KMAAMGEMIENIAHQWRQPLSVISTISSSLKIKKEMNILDDKEFYEALNNINKTSEHL
                     SNTIDDFRNFFSPNKEMNKFYVSQLIKKSKDLIKSRFDKFNIKVIEHIDDIEILSYQN
                     ELFQVILNLFSNSIDVLSSSQIENKIIYIKIYHDENNLYIEFLDNGGGIKDEFINRVF
                     EPYFTTKHKSQGTGIGLYMSLQIVTKHLNGEISVKNDTFIENNTAYFGAKFTILLPIH
                     LQKN"
BASE COUNT          527 a          199 c          177 g          594 t
ORIGIN      
        1 ataaaaatag tttttataaa aactaaagat aatttaattc ctattatttc aacaaatact
       61 aatttatctg atatagagat tatcaatact tacaaaaaaa gatggaatat tgaacaaggt
      121 tataaagatt tacgagaata tttccagttt ggtaaagaag agaacagaat ttatgaagct
      181 ctaattgcaa gaattacact ttcattttta gcttataaca taacaagcta tattaatcgt
      241 atcaataatg aaccccaaac attaggtaat ctttttagag atttagaatg ccaacttgaa
      301 accttagcta tttcaatgga gctattttta aaaatcctag aacaattgat acaatctgca
      361 caaatagtca agagaaataa agatttagag caaatcatcg agatactaag agttcacact
      421 aaaaaacagc tcggttttat gtgcgaaagt tgagtaagat aatatttaca aaagaagctt
      481 tagttctttt gtagatgaat aggtagcaat atagtaaatt ttgcaccaaa ataagcggta
      541 ttgttttcga taaaagtatc attttttaca cttatttcac catttagatg ttttgttacg
      601 atttgtagcg acatataaag accaattcct gttccttggc ttttatgttt tgttgtaaaa
      661 tatggttcaa aaactctatt tataaactca tcttttatgc ctccgccatt atctaagaat
      721 tcaatgtata aattattctc atcatgatat atttttatat atattatttt attctctatt
      781 tggcttgaag ataaaacatc aattgaattt gaaaaaaggt taagaattac ctgaaataat
      841 tcattttgat aggataaaat ctctatatca tctatgtgtt caataacttt tatattaaac
      901 ttatcaaatc tactttttat taaatcttta gattttttta ttagttgtga tacataaaat
      961 ttattcatct ctttatttgg agagaaaaaa tttctaaaat catcaatagt atttgataga
     1021 tgttcagatg ttttatttat attgtttaaa gcttcataaa actctttatc atctaaaata
     1081 ttcatctctt tttttatttt taaagagctt gatattgtag atattacaga taatggttgt
     1141 ctccattgat gagcaatatt ttcaatcatc tctcccattg cagccatttt agctttttga
     1201 tttaaaattg cctctttttc tagtaactct tttgtttttt tatctatagc taattgtaga
     1261 ttttcattta aatattttaa ctctttagtt ttttcctcta ctgtatattt taattttttg
     1321 tttaaaatat ttaaagttac tgttctgtga ataaatgcga taatgctaaa aaatactaca
     1381 aaagttaaaa tccaaactat tgtgtagtct attgaatttt gaaattttac attattccaa
     1441 ctattaacga tatttgtaac ttcagcctga ttcatatcat acctcacatc cgggtcc
//
LOCUS       DAOQAN010000352         1493 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_133625_length_1493_cov_2.251043, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000352 DAOQAN010000000
VERSION     DAOQAN010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1493
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133625_length_1493_cov_2.251043"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /gene="pgeF"
                     /locus_tag="PKO10_05855"
     CDS             <1..574
                     /gene="pgeF"
                     /locus_tag="PKO10_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014474140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HNW66705.1"
                     /translation="ALKLGYNYEDLIYMNQTHSSNIIVVDENSPKLVDNCDSIITRSK
                     NLPLMVMVADCIPILMFDDKKGIIAAIHAGRNSTFLEISKKTAEVFIEKFSSNPEDIN
                     AVFGASIQKCCYEVSEDLSKIVENSFGKEFVENNYIDLQGINKKQLNDLGVKNIEISN
                     ICTKCGDKPYFSYRKDKKTGRFAGIIILKD"
     gene            630..1475
                     /gene="purU"
                     /locus_tag="PKO10_05860"
     CDS             630..1475
                     /gene="purU"
                     /locus_tag="PKO10_05860"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014474142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HNW66706.1"
                     /translation="MEEYILKISTKDEKGLIYNISKVLFANNLNIDQNAEYVDEETNE
                     FFMRSLISGKVMETILQKELKEVLPKGAKITLNKNTKKDVVLLVTKESHVLGDLLIRY
                     SSNELNANIKAVIGNHEDLRNLVEKFDIPFFCISATNLSRDEHERAVSQKIDEFNPEI
                     IVLAKYMRILSSQFVEKYEGKVLNIHHSFLPAFIGANPYKQAFERGVKIIGATAHYVT
                     NDLDEGPIIFQDVVRVDHSYSWEDMRNAGRNVEKIVLSNAFELLLNDKVFIHKNKTVI
                     LERLH"
BASE COUNT          596 a          138 c          252 g          507 t
ORIGIN      
        1 ggctttaaag ctaggttata attatgaaga tttaatctat atgaatcaaa ctcatagttc
       61 aaatattatt gttgtagatg aaaatagtcc taaattagta gataattgtg actcaataat
      121 cacaagaagt aaaaatcttc cacttatggt tatggttgct gattgtattc ctattttgat
      181 gtttgatgat aaaaaaggaa taattgctgc tattcatgct ggaagaaact ctactttttt
      241 ggaaatttca aaaaaaacag ctgaagtttt tattgaaaaa ttttcttcaa atccagaaga
      301 tataaatgca gtttttggag cttctattca aaagtgttgt tatgaggtta gtgaagattt
      361 atcaaaaatt gtagagaact cttttggaaa agagttcgtt gaaaataatt acatagattt
      421 gcaaggaata aataaaaaac agttaaatga tttaggagta aaaaatatag agatatcaaa
      481 tatttgtaca aaatgtggag ataaaccata tttttcttat agaaaagata aaaaaactgg
      541 aagatttgct ggaataatta ttttgaaaga ttaactataa ttaataaaat ggtaaaatct
      601 aggttttaag ttttaaagat tggataaaaa tggaagaata tatactaaaa atttctacaa
      661 aagatgaaaa agggcttatt tataatattt ctaaagttct ttttgcaaat aatttaaata
      721 ttgaccaaaa tgctgagtat gtagatgaag agacaaatga gttttttatg agaagtttaa
      781 ttagtggaaa agttatggaa actattttgc aaaaagagct aaaagaggtt ttacctaagg
      841 gtgcaaaaat aactttaaat aaaaatacaa aaaaagatgt tgttcttctt gttacaaaag
      901 agagccatgt tttaggagat ttacttataa gatatagttc aaatgagcta aatgcaaaca
      961 tcaaagcagt tattggaaat catgaagatt taagaaattt agttgagaaa tttgatattc
     1021 catttttttg tataagtgct acaaatttga gtagagatga gcatgaaaga gcagtttctc
     1081 aaaaaattga tgagtttaat ccagagatta tagttttagc aaaatatatg agaatactat
     1141 cttcacagtt tgtagaaaaa tatgaaggta aagttttaaa tattcatcac tcttttttac
     1201 ctgcttttat tggagcaaat ccatataaac aagcatttga aagaggtgtt aaaattattg
     1261 gagcaactgc acattatgta acaaatgatt tagatgaagg tccaatcatt ttccaagatg
     1321 ttgtaagagt tgatcattca tatagttggg aagatatgcg aaatgctggt agaaatgttg
     1381 aaaaaattgt actttcaaat gcttttgaac tacttttaaa tgacaaagta tttattcata
     1441 aaaataaaac ggtgattttg gaaaggttac attaaacatt gaaaatttct taa
//
LOCUS       DAOQAN010000353         1487 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_134354_length_1487_cov_4.723464, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000353 DAOQAN010000000
VERSION     DAOQAN010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1487
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134354_length_1487_cov_4.723464"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..993
                     /locus_tag="PKO10_05865"
     CDS             <1..993
                     /locus_tag="PKO10_05865"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNW66707.1"
                     /translation="VSITNTPKHREEIARAFIAKSVYNIQTTRDLIDRLHSDRTLRIL
                     CGWRYKNDIPSESKFSRVFKELSALKIAQKTHEQFVKEYLRDTLFFYNASDATKIPLR
                     EKPVKVEKEKFKPKRRGRPKKGETREPKTPSILQQQEDMKTTKQMLSLVSTQCGVGRK
                     QNSKGNFETWIGGKLHISVVDGDIPITAIYSGANVHDSSVALPLINETSKKVSYLYDL
                     QDAGYDSNIIRDFSKKLNHRPLIDINPKNSKELKEKIQLIKDEKKKFEILNLTQSLDT
                     HHYNQRSMVERVNKYLKEDFGCSNIYYKGATKVASVLAFGILSVCIHQSLKMVT"
     gene            1097..1240
                     /locus_tag="PKO10_05870"
     CDS             1097..1240
                     /locus_tag="PKO10_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66708.1"
                     /translation="MIKSLKDLLDKFSRFTNSLVYDGGICKRLNLSKIKEWQTKQHEC
                     DYN"
     gene            complement(1273..>1487)
                     /locus_tag="PKO10_05875"
     CDS             complement(1273..>1487)
                     /locus_tag="PKO10_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011373196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNW66709.1"
                     /translation="RVIVQGLAEGSINATRVDIKPDGKVTGSIESEEFVIEAKGIFEG
                     NSLVKTSLIEKLKEKKEDFIEKEDIQ"
BASE COUNT          563 a          234 c          242 g          448 t
ORIGIN      
        1 gtatctatta caaacactcc aaagcataga gaagagattg cacgagcatt tattgcaaag
       61 agtgtttaca atatccaaac aaccagagac cttatcgata gacttcatag tgatagaacg
      121 ctgaggatct tgtgtgggtg gagatataaa aacgatattc caagtgagtc taaatttagt
      181 agagtattta aagagttaag tgctcttaaa attgcacaaa agacgcatga gcagtttgtg
      241 aaggagtatc taagggatac actctttttt tataatgcaa gtgatgcaac aaaaatacca
      301 ctgagagaaa aacctgtaaa agttgaaaaa gaaaagttta aaccaaaaag aagaggacga
      361 cctaaaaaag gtgaaacaag agagcctaaa acacccagta tcttgcagca acaagaagat
      421 atgaaaacca caaagcagat gctatctttg gtatcaacgc agtgtggagt tggaagaaaa
      481 cagaattcca aaggcaactt tgaaacatgg ataggaggga aactccatat cagtgtagta
      541 gatggagata tccctattac tgctatttat tcaggggcaa atgttcatga tagctcagta
      601 gcacttcctc ttataaacga gacaagcaaa aaagtatcat atctttatga cttacaagat
      661 gcaggatacg acagcaatat tatcagagat ttttccaaaa aactaaatca tagaccgctt
      721 attgatatca atccgaagaa ctccaaagag ttaaaagaaa aaattcaact tataaaagat
      781 gaaaaaaaga aatttgaaat tctaaacctt actcaaagtt tagataccca tcactataac
      841 caaagaagta tggttgagcg tgtaaataaa tacctcaaag aagactttgg atgcagtaat
      901 atttattata aaggagctac aaaagtagct tctgttttag catttggtat tttatcagtt
      961 tgtattcatc agagtttgaa aatggtaaca taacttttag ctaaaaacac taaaaattag
     1021 ccatttttaa aactcaacca tcaagcacgc acccaataat agttttttat ctaaaattga
     1081 cccataaagc ggtaaaatga ttaaaagttt aaaagatctg cttgataagt ttagtaggtt
     1141 tacaaattca ctagtttatg atggtggaat ttgcaagcgg ctcaatttat ctaaaataaa
     1201 agagtggcaa accaaacagc atgagtgtga ttataactaa aaatgtataa aagataattt
     1261 tatatgtttt tattattgta tatcttcttt ttcaataaaa tcttcttttt tctcttttaa
     1321 tttttcgatt aaacttgtct ttacaagact atttccttca aatatacctt ttgcttcaat
     1381 tacaaactct tctgattcaa ttgagcctgt aactttccca tctggtttta tatctactct
     1441 tgttgcattt attgagcctt ctgctaatcc ttgaacaata actctta
//
LOCUS       DAOQAN010000354         1487 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_134383_length_1487_cov_3.222765, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000354 DAOQAN010000000
VERSION     DAOQAN010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1487
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134383_length_1487_cov_3.222765"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..746
                     /locus_tag="PKO10_05880"
     CDS             <1..746
                     /locus_tag="PKO10_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HNW66710.1"
                     /translation="LFIEGGKGGLGNVHFKNSRNQRPTYFQPGLPGITKNIRLELKLI
                     ADVGLVGYPNVGKSTLISVTSNATPEIANYEFTTLTPKLGVVNVGDYSSFVMADIPGI
                     IDGASEGKGLGLEFLKHIERTKTLLFTIDVANHRTMIDQYNVLKEELFKFSQELSQRD
                     FAIVLSKIDAYYGEDLQNDIEEFLKALNLETSKSNEYKFDTNLPYYVQDLIFTKKDSS
                     KPYFVLPISSLDKTNINPLKYALYTMLGK"
     gene            747..>1487
                     /gene="proB"
                     /locus_tag="PKO10_05885"
     CDS             747..>1487
                     /gene="proB"
                     /locus_tag="PKO10_05885"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014474790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HNW66711.1"
                     /translation="MKRLVIKVGTAVLTQGEELALQRMKNLVNLISTLKNDKNLEVIL
                     VSSGAVGAGYTELKLDKTVLANKQTLAAIGQPKLMKTYQEMFQEFGITVAQMLFIADD
                     FDSRKRSKNAKNVMEILLQNKVIPIINENDVIATEELIGDNDQLAAYITHYFKADMLV
                     ILTDIDGYYDKNPREFSDAKIQKIINEISQDELDKKPSANSKFATGGIVTKLKAADFL
                     MQKNIPMYLTSGFDLTNAYDFLVDNNHKS"
BASE COUNT          597 a          170 c          243 g          477 t
ORIGIN      
        1 ttttatttat tgaaggtgga aaaggtggac ttggaaatgt tcattttaaa aactcaagaa
       61 atcaaagacc tacatatttt caaccaggat tgccaggaat tacaaaaaat ataagattag
      121 agcttaaatt aattgcagat gttgggcttg ttggttatcc aaatgttgga aaatctactt
      181 taatatctgt aacatcaaat gcaactccag aaatagcaaa ctatgagttt acaacattaa
      241 ctccaaaatt aggagttgta aacgttggag attatagctc atttgtaatg gctgatattc
      301 ctggaattat tgatggtgca agtgaaggaa aagggcttgg attagagttt ttaaaacata
      361 ttgaaagaac aaaaactctt ttatttacaa tagatgttgc aaatcataga acgatgattg
      421 atcaatataa tgtattaaaa gaagaacttt ttaagttttc acaagagctt tcacaaagag
      481 attttgcaat agttttaagt aaaatagatg cttattatgg tgaagattta caaaatgata
      541 tagaagaatt tttaaaagct ttaaatcttg aaacttctaa atcaaatgag tataaatttg
      601 atacaaattt accatactat gttcaagatt taatttttac aaaaaaagat agtagtaaac
      661 catattttgt tttaccaata tcatctttag acaaaacaaa tattaatcca ttaaaatatg
      721 ctttatatac aatgctagga aaatagatga aacgattagt tatcaaggtt ggaactgctg
      781 ttttaactca aggtgaagag ttagctttgc aaagaatgaa aaatctagtt aatctaattt
      841 caacactaaa aaatgataag aatttagagg taatactagt aagctcagga gcagttggag
      901 caggatatac agagctaaaa cttgataaaa cagttctagc aaataaacaa actttagctg
      961 ctattggtca accaaaatta atgaaaactt atcaagagat gtttcaagaa tttggtatta
     1021 ctgttgctca aatgcttttt attgcagatg attttgattc aagaaaaagg tcaaaaaatg
     1081 caaaaaatgt tatggagata ttgcttcaaa ataaggtaat tcctataata aatgaaaatg
     1141 atgttattgc aactgaagag ctaattggag ataacgacca gttagcagct tatataacac
     1201 actattttaa agctgatatg ttagttattt tgacagatat agatggatat tatgataaaa
     1261 atccaagaga gtttagtgat gcaaaaattc aaaaaataat aaatgaaata tcacaagatg
     1321 agcttgataa aaaacctagt gcaaactcaa aatttgcaac aggtggaatt gttaccaaac
     1381 taaaagctgc tgatttttta atgcaaaaaa atattccaat gtatttaact agtggttttg
     1441 atttgacaaa tgcttatgat tttttagttg ataataatca taaaagt
//
LOCUS       DAOQAN010000355         1487 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_134453_length_1487_cov_2.192039, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000355 DAOQAN010000000
VERSION     DAOQAN010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1487
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134453_length_1487_cov_2.192039"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="PKO10_05890"
     CDS             <1..332
                     /locus_tag="PKO10_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014473902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNW66712.1"
                     /translation="SIDDIAGVVFKQTILEKRVKKKKKTLLKDIIVPVHKVPENISVS
                     TLFDMFIRMKMHLFIVQDEYGQTSGVVTLEDALETLLGIEIVDEMDQVTDMQEFAKDE
                     NKRLQRL"
     gene            347..1414
                     /locus_tag="PKO10_05895"
     CDS             347..1414
                     /locus_tag="PKO10_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNW66713.1"
                     /translation="MNSLNTEINFARAFYFFAFMFLVIYSFNSLSNILTPIAISFFIW
                     FLINGLANEIKKIPYINKRVLDFVATPLSLIIILYSMVKIGTFVTSSMIELGSVVAQL
                     DSKINVVIDKISTLTSFDLKEPLQKFFQEFNMATLLNRIFVAFSSILSNTMQIFLYVL
                     FLLIDQRFFDLKLNALFQNENSKKKAKDILTSISKGVRTYILITTIVSLITGFLTFLI
                     CQYFGLQGAVLWGFIAFILNFIPTIGTIIAVLIPTVFAIIQLTDLSSILYLFIFLVLI
                     QFVIGNVIYPKLMGKSLNISQFVVILSLVVWGAMWGTIGMFLAVPIMMILLIILSQFE
                     STKNLAVLISEDGNILRNNDN"
BASE COUNT          529 a          149 c          227 g          582 t
ORIGIN      
        1 aatcaataga tgatatagca ggagttgttt ttaaacaaac tattttggaa aaaagagtaa
       61 aaaagaagaa aaagactctt ttaaaagata ttattgttcc tgttcataaa gttccagaaa
      121 atatatctgt atcaactctg tttgatatgt ttattagaat gaaaatgcat ctttttattg
      181 ttcaagatga gtatggtcaa acaagtggag ttgtaactct tgaagatgct ttagagacac
      241 ttttaggaat tgaaattgtt gatgagatgg atcaagttac agatatgcag gagtttgcaa
      301 aagatgagaa taaaagattg caaagattat aaaaaaagga gtgtatatga atagcttaaa
      361 tacagagata aattttgcta gagcatttta tttttttgct tttatgtttt tagtgattta
      421 ctcttttaat agtttaagca atattctaac gccaattgca atatcttttt ttatttggtt
      481 tttgataaac ggtttagcaa atgagataaa aaaaatacca tatataaata agagagtttt
      541 agattttgtt gcaactccat tatctcttat aatcatattg tattctatgg tgaaaatagg
      601 aacttttgtg acttcaagta tgatagagtt aggttctgta gtagctcaac ttgactcaaa
      661 aataaatgta gtaatagata aaatatcaac tttgacatct tttgatctta aagaaccttt
      721 gcaaaaattc tttcaagagt ttaatatggc tacattatta aatagaattt ttgttgcttt
      781 tagttcaatt ttgagtaata ctatgcagat atttttatat gttctttttt tattaattga
      841 ccagagattt tttgatttaa aattaaatgc actatttcaa aatgaaaata gcaaaaaaaa
      901 agctaaagat atattaacat ctatttcaaa aggagtaaga acttatattt taattacaac
      961 aatagttagt ttaattacag gttttttaac tttcttaata tgtcaatatt ttggtttaca
     1021 aggtgcagtt ttatggggat ttatagcatt tattttaaat tttattccaa caattggaac
     1081 tataatagct gttttaattc caacagtttt tgccattatc caactaacag atcttagctc
     1141 aattttgtat ctatttatat ttttagtatt aattcaattt gtgattggta atgtaattta
     1201 tccaaaactt atgggaaaga gcctaaatat atctcaattt gtagtaattt tatctttggt
     1261 tgtttgggga gctatgtggg gaactattgg tatgtttttg gctgttccta ttatgatgat
     1321 tttattgatt attttatctc aatttgaaag tacaaaaaat ctagcagttt taatttccga
     1381 agatggaaat attttaagaa acaatgataa ttagagttgt agcaatactt ttcttactaa
     1441 tatttgcatc ttttgaagca aaaaaattta ttagtaaaag taaaaat
//
LOCUS       DAOQAN010000356         1487 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_134472_length_1487_cov_1.985335, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000356 DAOQAN010000000
VERSION     DAOQAN010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1487
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134472_length_1487_cov_1.985335"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="PKO10_05900"
     CDS             complement(<1..521)
                     /locus_tag="PKO10_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012013164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HNW66714.1"
                     /translation="MMLLKSIPKVDKFITNKAFEGLSKTLITKITKEVLENLRVEILN
                     KKIDSFDEQTLISNVLRKYENLTQSSLKPLINATGIIVHTNLGRSLLDESLFKNAITI
                     ATSYNNLEYSLEEGKRGERYEHITKSLQALTSCEDALVVNNNASAVFLILNTFCKNKE
                     AVLSRGELVEIGG"
     gene            complement(692..>1487)
                     /locus_tag="PKO10_05905"
     CDS             complement(692..>1487)
                     /locus_tag="PKO10_05905"
                     /inference="COORDINATES: protein motif:HMM:NF014324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HNW66715.1"
                     /translation="EPKKEFFEHNIDKSKDKETNDNLEQLVTKDFGIYPYKKNYFLPA
                     TYTFNNISNRDNFETSFQISLEKPISNDFFGLNETISIAYTQKSFWQTASSSAPFRET
                     NYEPEIFMQIPNDDKYLKLYKTSFLHTSNGKGGDDSRSLNRLYLQTFFQFDNLFVSPK
                     IWYKIPQNSKDDDMKDFYKYYGYGDISFLYAYGKQTFELLLRDNLRLNSSNKGAALFN
                     YTFPLPDFISSKNSYGIFQVFHGYGQSLIDYDREVTNIGIGLTFSR"
BASE COUNT          491 a          220 c          201 g          575 t
ORIGIN      
        1 cttccaccaa tttcaacaag ctctccacgg ctaagaacag cctctttatt tttacaaaat
       61 gtattaagga ttagaaaaac agcacttgca ttgttgttta caaccaaagc atcttcacaa
      121 gaagtgagtg cttgtagaga ttttgtgatg tgttcgtatc tctctcctct ttttccctct
      181 tctaaactat attcaagatt attataagat gttgctattg ttattgcatt tttaaatagg
      241 ctctcatcaa gcaaacttct tcctaagttt gtatgcacaa taattccagt tgcgtttata
      301 agtggtttta gtgaagattg tgtaagattt tcgtactttc ttaaaacatt tgaaataaga
      361 gtttgttcat caaaactatc tatcttttta tttaatattt caactctcag attttctaaa
      421 acttcttttg taatttttgt tattaaagtt tttgataatc cttcaaaagc tttgtttgtg
      481 ataaacttat caacctttgg aatggatttt agtaacatta tagacctttt atactatttt
      541 aaagagtatt attatactat tttaatctta tggagagttt gtgtatctgg tgagcaccac
      601 aggcttcaac cctgattgac ggtttttgtg taaacgccgt gagaggttcg attcctctac
      661 tctctcgcca taagaaagta taaaataagt tttatctaga gaaagtaagt ccaataccaa
      721 tatttgttac ttctctatca taatctatca aactttgacc atatccatgg aaaacttgaa
      781 aaataccata actatttttt gaagatataa aatctggaag aggaaaagtg tagttaaaaa
      841 gagctgcccc cttgtttgaa ctatttaatc tcaaattatc tcttaagagt agttcaaaag
      901 tttgtttacc ataagcatac aaaaatgaaa tatctccata tccataatat ttataaaaat
      961 ctttcatatc atcatcttta ctattttggg gaattttata ccaaattttt ggagacacaa
     1021 aaagattatc aaattgaaaa aatgtttgta aatataatct atttaaagat cttgaatcat
     1081 cgccaccttt tccatttgaa gtgtgtaaaa atgatgtttt ataaagcttt aagtatttat
     1141 catcattagg aatttgcata aaaatttctg gttcatagtt tgtttcacga aatggagcag
     1201 aactagaagc agtttgccaa aatgattttt gagtatatgc aatacttatt gtctcgttta
     1261 aaccaaaaaa atcattagaa attggttttt ctaaactaat ttgaaaagaa gtttcaaaat
     1321 tatctctatt tgaaatattg ttaaaagtat atgttgctgg taaaaaatag ttctttttat
     1381 aaggatatat accaaaatct tttgtaacta gttgctctaa attatcattt gtctctttat
     1441 ctttagattt atctatatta tgttcaaaaa actctttttt tggctct
//
LOCUS       DAOQAN010000357         1485 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_134703_length_1485_cov_2.891608, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000357 DAOQAN010000000
VERSION     DAOQAN010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134703_length_1485_cov_2.891608"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            8..715
                     /locus_tag="PKO10_05910"
     CDS             8..715
                     /locus_tag="PKO10_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013136667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HNW66716.1"
                     /translation="MKNILFLFIIYLLFTSCGALTEPKIDFTKPPQQVTKEPPIVRKN
                     KGSLYSVQGTSLFADKKDLQVGDIIQVVISEGITQKSDNKRELTSDRKNEFGGGMFAS
                     MGGNPLNSTVGRVTDKANSVFGVNFNTESSDSDKGKVKTQVNENFDTKISAIIDETYQ
                     NGNYFIKGNKEVIIDGQKQEIVITGVIRPYDITSDNSINSSQIANLKMLYKKDGAEAD
                     LLQVPWGTKILRAIWPF"
     gene            770..>1485
                     /locus_tag="PKO10_05915"
     CDS             770..>1485
                     /locus_tag="PKO10_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HNW66717.1"
                     /translation="MFSTLSVSQTGLNTSKYAIENVSNNQANRNTPGYKKRVADLSEI
                     RLNGVHITGQGVNFGGISRVTSQYMYDKFMQESTKANYLDKSSNMLGGIEKIFAETDS
                     SGFSVDLNRYFQSVESLRTNPSSEVNRSYMKTQGAVIVESLQNLYSSIEKQAQIEKVE
                     LKTDVKKVNQILKEIADVNVKIEKYDPSVNDLLDKRDLLELELSKFVDVDINRDAGFY
                     EIKIGGVVAVSNNIFHKEIE"
BASE COUNT          620 a          150 c          257 g          458 t
ORIGIN      
        1 aattaggatg aaaaatattt tatttttatt tataatctat ttgttattta catcttgtgg
       61 agcattaact gaaccaaaaa tcgattttac aaaaccacca caacaagtaa caaaagaacc
      121 tcctattgtt agaaaaaata aaggttcttt gtattctgtt caaggaacat ctctttttgc
      181 tgataaaaaa gatttacaag ttggagatat tattcaagtt gtaataagtg aaggaattac
      241 acaaaaaagt gataataaaa gagagttaac tagcgataga aagaatgaat ttggtggagg
      301 aatgtttgct tctatgggag gaaatccact aaatagtaca gttggaagag taacagataa
      361 agcaaattct gtatttggag taaattttaa tacagaaagt agcgatagtg ataaaggaaa
      421 agtaaaaact caagtaaatg agaattttga tacaaaaatt tcagctatta tagatgaaac
      481 ttatcaaaat ggtaactatt tcataaaagg aaataaagag gtaataattg atggtcaaaa
      541 acaagagatt gtaattacag gtgttataag accatacgat ataacatctg ataattcaat
      601 aaactcttca caaattgcaa atttaaaaat gctttataaa aaagatggag cagaagctga
      661 tttactgcaa gttccatggg gaacaaaaat cttaagagca atatggccat tttaaactaa
      721 aattaagaaa tgtaatgcaa taattttaca tattataaaa ggagtaaata tgttttctac
      781 attgagtgtt tctcaaacag ggcttaacac atcaaagtat gctattgaga atgttagtaa
      841 caatcaagca aatagaaata ctcctggata taaaaaaaga gtagcggatt taagcgaaat
      901 tagattaaat ggtgtacata taacaggaca aggtgttaat tttggtggaa tatcaagagt
      961 tacatctcaa tatatgtatg acaagtttat gcaagagagt acaaaagcta attatttaga
     1021 taaatcttca aatatgttag gtggtattga aaaaattttt gcagaaactg atagtagtgg
     1081 tttttcagtt gatttaaata gatattttca atctgtagaa agtctaagaa caaatcctag
     1141 ttctgaagta aatagaagtt atatgaaaac tcaaggtgct gtaattgttg aatctttaca
     1201 aaacttatat tcaagtatag aaaaacaagc acaaatagaa aaagttgagt taaaaactga
     1261 tgttaaaaaa gtaaatcaga ttttaaaaga gattgcagat gtaaatgtaa aaattgaaaa
     1321 atatgaccca tctgtaaatg atttactaga taaaagagat ttgctagaac ttgaactatc
     1381 aaaatttgtt gatgttgata taaatagaga tgctggtttt tatgagataa aaatcggtgg
     1441 agttgttgct gtttcaaata atatttttca taaagagata gaaat
//
LOCUS       DAOQAN010000358         1484 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_134831_length_1484_cov_3.362491, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000358 DAOQAN010000000
VERSION     DAOQAN010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1484
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134831_length_1484_cov_3.362491"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            148..723
                     /gene="grpE"
                     /locus_tag="PKO10_05920"
     CDS             148..723
                     /gene="grpE"
                     /locus_tag="PKO10_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HNW66718.1"
                     /translation="MSELKQDEVLNEEIEKTESCCESSKECCEGKEEQKEESLEDVIA
                     RLEDELKQSEDKFLRVHADFENIKKRLEREKYQAIDYASEKFAKDLLTPLDTLEMALN
                     SANANLSAEELLPKLKEGIELTIKSFITTFEKHNITKVDTNCEFDPNVHNAVMQVDSP
                     DHETGQIVSELQKGYMLKDRLLRPSMVSIAN"
     gene            805..>1484
                     /locus_tag="PKO10_05925"
     CDS             805..>1484
                     /locus_tag="PKO10_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HNW66719.1"
                     /translation="MSKVIGIDLGTTNSCVAVYENGEAKIIPNKEGKNTTPSIVAFTD
                     KGEVLVGDPAKRQAITNPEKTIYSIKRIMGLMMNEPNAKEAQSKVGYKIVDRNGAAAV
                     EIAGKVYTPQEISAKILGKLKADAEEYLGSSVTDAVITVPAYFNDAQRKATQEAGTIA
                     GLNVLRIINEPTAASLAYGLDKKGEEKVLVYDLGGGTFDVTVLEIGDGTFEVLSTDGN
                     AFLGGDDF"
BASE COUNT          602 a          150 c          296 g          436 t
ORIGIN      
        1 tttgatggtt ttttaccaca aagttatata ggagtttgta aattctgcaa aatcaataac
       61 gaagattata aaatgtttgt tgttggtgaa ttatcaaaag attatgaata tttttttgaa
      121 caaattataa atttttaagg agataaaatg agtgaattaa aacaagatga agttttaaat
      181 gaagagatag aaaaaacgga aagttgttgc gaaagttcaa aagagtgttg tgaaggaaaa
      241 gaggaacaaa aagaggagag tttagaagat gttattgcta gacttgaaga tgaattaaaa
      301 cagagtgaag ataaattttt aagagttcat gctgattttg aaaatattaa aaaaagatta
      361 gagagagaaa aatatcaagc aatagattat gcaagtgaga agtttgcaaa agatctatta
      421 actcctcttg atactttgga aatggcttta aattcagcaa atgcgaattt gagtgctgaa
      481 gagttacttc caaaactaaa agagggaata gagcttacaa ttaaaagttt tattacaaca
      541 tttgaaaaac acaatatcac aaaagttgat acaaattgtg agtttgatcc aaatgttcat
      601 aatgcagtta tgcaagttga tagtcctgat catgaaactg gacaaattgt aagtgagtta
      661 caaaaagggt atatgttaaa agatagattg cttagacctt caatggttag catagcaaac
      721 taaaatttga ccgaaatgtc attaaactat agaatttaag attatttaca aattaaaaag
      781 aaaatttaat aaaaggaaga aaaaatgagt aaagttattg gtatagattt aggaacaaca
      841 aactcttgtg ttgcagttta cgaaaatggt gaagcaaaaa ttattccaaa taaagaaggg
      901 aaaaatacaa ctccttctat tgttgctttt acggataaag gtgaagtact tgtaggagat
      961 cctgcaaaaa gacaagctat tacaaatcca gaaaaaacta tatattctat aaaaagaatt
     1021 atgggactta tgatgaatga gccaaatgct aaagaggctc aaagtaaagt tggatataaa
     1081 atagttgata gaaatggtgc agctgctgtt gagattgctg gaaaagttta tactcctcaa
     1141 gaaatttcag caaaaatttt aggaaaatta aaagcagatg ctgaagagta tttaggaagt
     1201 agtgttacag atgctgttat tacagttcct gcatatttta acgatgcaca aagaaaagca
     1261 actcaagaag ctggaacaat tgcaggtctt aatgttttaa gaattataaa tgagccaaca
     1321 gcagcatcac tagcatacgg acttgataaa aaaggtgaag agaaagttct agtttatgat
     1381 ttaggtggag gaacatttga cgttacagtt cttgaaattg gagatggaac ttttgaagtt
     1441 ttaagtacag atggaaatgc tttcttaggt ggagatgatt ttga
//
LOCUS       DAOQAN010000359         1482 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_135113_length_1482_cov_3.280308, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000359 DAOQAN010000000
VERSION     DAOQAN010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135113_length_1482_cov_3.280308"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(182..394)
                     /gene="rpsU"
                     /locus_tag="PKO10_05930"
     CDS             complement(182..394)
                     /gene="rpsU"
                     /locus_tag="PKO10_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002343807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HNW66720.1"
                     /translation="MPGIKVRDNESFDEAYRRFKKQCDRNLIVTETRARRYFEPMTEI
                     RKKQKISARKKMLKKLYMLRRYESRL"
     gene            509..979
                     /gene="moaC"
                     /locus_tag="PKO10_05935"
     CDS             509..979
                     /gene="moaC"
                     /locus_tag="PKO10_05935"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012148102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HNW66721.1"
                     /translation="MNLTHIDDNNRPKMVDVSGKNETHRIAVASGKITMSKEAYDAII
                     GNVVKKGPVLQTAVIAAIMGTKKTSELIPMCHPLLLSGINCDIEELPQTNSFKLFVTA
                     KLNGQTGVEMEALTGVSVGLLTIYDMVKAIDKSMIISDIQLESKSGGKSGDFKR"
     gene            976..1239
                     /locus_tag="PKO10_05940"
     CDS             976..1239
                     /locus_tag="PKO10_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 domain-containing protein"
                     /protein_id="HNW66722.1"
                     /translation="MIDLSDKKLELNYPCSWEYKLVVLETCNIKKCVKEVIFEREHNL
                     KPSKTSSKGKFKSYSLEMLVHNDDDRTEIFRLLGEHSDIKMVL"
     gene            complement(1244..>1482)
                     /locus_tag="PKO10_05945"
     CDS             complement(1244..>1482)
                     /locus_tag="PKO10_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013136833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66723.1"
                     /translation="HITKLVGTLKNEDELQEVLKRKFTKREYKTFIAFEEGKNIDEIK
                     TLLKEEDTKEVEKIYQTAIKKLNQEIFKRELVDL"
BASE COUNT          507 a          183 c          231 g          561 t
ORIGIN      
        1 ttatatctct tttgatgaac ttttaccagc ttcaagagtc tatggaaatg ctcatactac
       61 aatagttggt atctctttgg gaatgtttgt aatggctatt agcttagtgt tgtttaagtt
      121 aatctaaata aaaaaagccc aattcctaag aactgggctt ttacttggaa aataataaat
      181 cttataatct agattcgtat cttctaagca tatataattt tttaagcatt ttctttctag
      241 cagaaatttt ttgttttttt ctgatttcag tcattggttc aaaatatctt ctagctcttg
      301 tttctgtaac tataaggttt ctatcacatt gctttttaaa tcttctatac gcttcgtcaa
      361 aagattcatt atctctaact ttaatgcctg gcactaaaat cacccacttt ctttttaaat
      421 ttgagcttgg attataatta aataaactta attcctattt aatctatatt agaatagaat
      481 aattttttta aatattaagg aatatattat gaatttaaca catattgatg ataataatag
      541 accaaaaatg gtagatgttt caggaaaaaa tgagacacat agaattgcag ttgcaagtgg
      601 aaagattact atgagtaaag aggcttatga tgcaattatt ggaaatgttg ttaaaaaagg
      661 tccagtttta caaacagccg taatagctgc aattatgggg acaaaaaaga caagcgaatt
      721 aatacctatg tgccatcctt tacttttgag tggaattaac tgtgatatag aagagttacc
      781 acaaacaaat agttttaaac tttttgttac agcaaaatta aatggacaaa caggtgttga
      841 gatggaagct ttaacaggtg ttagtgttgg acttcttaca atttatgata tggtaaaggc
      901 tattgataaa agtatgataa tttcagatat tcagcttgaa agtaaaagcg gtggaaaaag
      961 tggagatttt aaaagatgat agatttaagt gataaaaaat tagagttaaa ctacccttgt
     1021 agttgggaat acaaacttgt tgttttggaa acttgtaata ttaaaaaatg tgtaaaagag
     1081 gttatttttg aacgagaaca taacttaaaa ccatcaaaaa ctagttcaaa aggtaaattc
     1141 aaaagttata gtttggagat gctagtacac aatgatgatg atagaactga gatttttaga
     1201 ctattaggtg agcacagtga tataaaaatg gtgttgtaga tttttataaa tctactaact
     1261 cccttttaaa aatctcttga tttaattttt taatagcagt ttggtagatt ttttctacct
     1321 cttttgtatc ttcttctttt aaaagagttt ttatttcatc tatattttta ccctcttcaa
     1381 aagctatgaa agttttatac tctctttttg taaattttct ttttaatact tcttgaagtt
     1441 catcttcatt ttttagagtt cctacaagtt ttgttatatg tt
//
LOCUS       DAOQAN010000360         1482 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_135142_length_1482_cov_2.763139, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000360 DAOQAN010000000
VERSION     DAOQAN010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135142_length_1482_cov_2.763139"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..786)
                     /locus_tag="PKO10_05950"
     CDS             complement(<1..786)
                     /locus_tag="PKO10_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HNW66724.1"
                     /translation="MKDITLVVLCAGNSTRFDNLCKKQWIRIDNEPLWLNVTNRLTSF
                     SNFSKTIVVSHKDELSYMKNFSDDFIYVEGGDTRQNSIKNALEFVDTPYVMISDVARA
                     CISKNIISDLIQNKNKASCIVPILDVSDTVIFEKNTINREDVKLIQTPQLSNSDILRK
                     AISKDIEFTDESSAIKNIGEDIFYIKGDIASKKLTFFEDISQIPCLKAPAKTQFVGIG
                     YDIHSFEDKKEMFLGGVKLPYDYGFKAHSDGDVLIHSLIDSLLG"
     gene            complement(845..>1482)
                     /locus_tag="PKO10_05955"
     CDS             complement(845..>1482)
                     /locus_tag="PKO10_05955"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNW66725.1"
                     /translation="LNKPKNIHNFILITSLEFVCALATSLSFCRNIKTNLFSYAATFD
                     DFLYSNTLSMLIVEMWLKKSDKKLKDELLIPAFLQDLAKPFISQAISENKLTEQFLNE
                     IKNSNMSKAEEKFIGFNSQRISSTILKNWGLSHNIIFPILFSKDLKNCPDEFRLKALI
                     LNIVSIVSNLREPLLESNIKKAINEIELFGLNSDTFLSTISNINDNIKSNS"
BASE COUNT          530 a          212 c          168 g          572 t
ORIGIN      
        1 acctaaaagg gaatcaatta aagagtggat taaaacatcg ccatcactgt gggctttaaa
       61 tccataatca tatggcaatt ttacaccacc taaaaacatc tcttttttat cttcaaagct
      121 atgaatatca tatccaattc caacaaattg tgttttagct ggagctttta aacatggaat
      181 ttgtgaaata tcttcaaaaa aagttagttt tttactagcg atatcacctt ttatataaaa
      241 tatatcttca cctatatttt ttatagcact actttcatct gtaaattcaa tatcttttga
      301 aattgctttt cttagaatat cgctatttga taactgagga gtttgaataa gttttacatc
      361 ttccctatta atagtgtttt tctcaaatat aacagtatca cttacatcta aaattggaac
      421 tatacaagag gctttgtttt tattttggat taaatcagaa ataatatttt tgctaataca
      481 agcacgtgca acatcgctta tcattacata aggtgtatca acaaattcta aagcattttt
      541 tatagaattt tgtcttgtat ctccaccttc aacataaata aaatcatcac taaagttttt
      601 catgtagctt aactcatctt tatgtgaaac gacaatagtt tttgaaaaat tagaaaaaga
      661 agttaatcta tttgtaacat ttaaccacag aggttcattg tcaatcctaa tccactgttt
      721 tttacataaa ttatcaaatc ttgtagagtt tccagcacat aaaacaacta aagtaatatc
      781 tttcaattat aaacctttga gtaaaaaagt tacatatagt agctaaatgt tacttataat
      841 acttttaaga attgctcttt atattatcat ttatgttact tattgtactt aaaaatgtat
      901 cgctatttaa cccaaataac tcaatttcat taattgcttt ttttatattt gattctaata
      961 gaggctctct taaatttgaa acaatgctaa caatattcaa gataagagct tttagtctaa
     1021 attcatctgg acaatttttt aaatcttttg aaaataaaat tggaaatata atattgtgac
     1081 ttaaacccca atttttaagt atagtagagc ttattctttg agaattaaat cctataaatt
     1141 tctcttcagc tttactcata tttgagtttt ttatttcatt taagaactgt tcggttagtt
     1201 tattttcact tattgcttga gagataaaag gttttgccag atcttgtaaa aatgctggaa
     1261 taagaagttc atcttttaac tttttatcac ttttttttaa ccacatttca acaattagca
     1321 ttgaaagagt atttgaatat aaaaaatcat caaaagtggc agcatatgaa aatagatttg
     1381 tttttatatt tctacaaaaa gataaagatg ttgctaatgc acaaacaaac tctaaactag
     1441 taattaaaat aaaattatgt atattttttg gtttattcaa aa
//
LOCUS       DAOQAN010000361         1482 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_135160_length_1482_cov_2.556412, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000361 DAOQAN010000000
VERSION     DAOQAN010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135160_length_1482_cov_2.556412"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..1308)
                     /locus_tag="PKO10_05960"
     CDS             complement(115..1308)
                     /locus_tag="PKO10_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNW66726.1"
                     /translation="MLVLKSSGIDLSKSETRTIIGFSLIYSILVLVILGVITFLYYQF
                     KKDLMLQDVRQTLQNYSNTQIANLKELHINIDKSDIYPRDERFNSAIYDSSKKKIFST
                     LLMGDVKLDEVIYLKDGYIHLIKEPESYYLGSKYVIVEIEDDNIWFANIKYKMLFWFL
                     FSFILLLFVGYFIAKLFLKPMRESIQMLDRFIKDTTHELNTPIAAILSNIQMINKDNI
                     DEKLAKKINRIEIGAKTISNIYEDLTFVSLNNQIISNNEKLNFSQILNQRVDFFKSIA
                     NSKQIEFILDIKDNVFIVCDIKKLSKLIDNILSNAIKYNKFQGFIKVTLKDKILIIED
                     SGKGMSKDNLSNLFTRYKRFDKSVGGFGIGLNIVSLIAKEYDLKIDVISKIDVGTRIK
                     IRWQD"
     gene            complement(1265..>1482)
                     /locus_tag="PKO10_05965"
     CDS             complement(1265..>1482)
                     /locus_tag="PKO10_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNW66727.1"
                     /translation="KLNLKELKLLKFFLQNPNELLSHDRIYDFVWDYDEEYSDNSLRT
                     YIKNLRKILGKDKIVSFKKLGYRFIQE"
BASE COUNT          554 a          210 c          128 g          590 t
ORIGIN      
        1 aaaagtattt atctatagta acaggaattc tattgtgaca agtcacacaa tttctttcat
       61 aaatattttt tggattagaa aaagcaaaaa tagatgttaa acataaaaaa atcactaatc
      121 ttgccatctt atttttatcc ttgttcctac atcaattttt gaaataacat caattttcaa
      181 atcatactct tttgctataa gagatacaat attaagccct attccaaagc cacctacact
      241 tttatcaaat cttttatatc ttgtaaaaag atttgataaa ttatctttac tcataccttt
      301 tccactatct tcaattatca aaattttatc ttttaaagta acttttataa atccttgaaa
      361 cttattatat tttattgcat tagaaagaat attatctatt agttttgata gtttttttat
      421 atcacaaact ataaaaacat tatcttttat atctaaaata aactcaattt gtttactatt
      481 agcaattgat ttaaaaaaat caactctttg atttaaaatt tgagaaaaat tcaacttttc
      541 attatttgag attatctgat tatttaaaga gacaaaagtt aaatcttcgt atatatttga
      601 aattgttttt gcacctattt caattctatt tatctttttt gctaactttt catcaatatt
      661 atctttattt atcatctgta tatttgataa aattgcagca attggagtat ttaactcatg
      721 agtagtatct tttataaatc tatcaagcat ttgtatagac tctctcattg gttttaaaaa
      781 tagttttgca ataaaatatc caacaaatag aagcaaaata aatgaaaata gaaaccaaaa
      841 aagcatttta tatttaatat ttgcaaacca aatattatca tcttctatct caacaattac
      901 atatttacta cctaaatagt aagattctgg ctctttgatt aaatgaatat acccatcttt
      961 taaatatata acttcatcta gttttacatc acccataagt aaagttgaaa aaattttctt
     1021 ttttgagcta tcataaatag ccgaattaaa tctttcatct cttggatata tatctgattt
     1081 atcaatatta atatgtaact cttttaaatt tgcaatttga gtatttgagt agttttgaag
     1141 agtttgtcta acatcttgaa gcattaaatc ttttttaaat tgataatata aaaaagtaat
     1201 aacacccaaa ataaccaaaa ctaatattga gtaaattaaa ctaaacccaa taatagttct
     1261 agtttcactc ttggataaat ctatacccga gctttttaaa actaacaatt ttatcttttc
     1321 ccaaaatctt tctaaggttt tttatataag ttcttaaaga gttatcacta tactcttcat
     1381 cataatccca aacaaaatca taaattctat cgtgagagag tagttcattt ggattttgaa
     1441 gaaaaaattt caaaagcttt aactctttta aatttaattt ta
//
LOCUS       DAOQAN010000362         1482 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_135190_length_1482_cov_2.128942, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000362 DAOQAN010000000
VERSION     DAOQAN010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135190_length_1482_cov_2.128942"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="PKO10_05970"
     CDS             complement(<1..315)
                     /locus_tag="PKO10_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66728.1"
                     /translation="MGHIENELDKLYSNIFNKDELRKNFSTAEISKISAPFLLNIDEE
                     KYLNSKTKILFIGKETNKWWGKLKHFIEFDNSIEIMKQRYKAEFEGGDVIASDGIGDL
                     DGV"
     gene            complement(472..>1482)
                     /locus_tag="PKO10_05975"
     CDS             complement(472..>1482)
                     /locus_tag="PKO10_05975"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNW66729.1"
                     /translation="IFFDDLSHRARYLESILKEELDIFKDKLVQLEERSGLFDSSTDI
                     QLTPKAIALLLQSKDKNKKQEFKAQFTKHIKFNSLKKEIFLDERVARDINQLKDVCSS
                     KNFNKIVKDLKKANLPSGIVSIFYGFAGTGKTASVYEIAKLTKRDVLQVDISSIQSKW
                     VGESEKNTKAIFQEYYRACEILKSKPILLFNEADAIISKRLDVNDAVSQMNNTMQNIL
                     LEELENFDGIFMATTNLIDNMDDAFSRRFLNKIKFDRPTAKTREHIWKSKLPELEDKI
                     YEKLSIYDLSGGQIENVTRKYLINKILNQKEFDFDEILEYIKEEISFKNDDNSLKVGF
                     LK"
BASE COUNT          486 a          240 c          129 g          627 t
ORIGIN      
        1 tacaccatct aaatccccta tcccatcaga agctattaca tccccacctt caaattcagc
       61 tttatatctt tgtttcataa tttcaataga attatcaaat tctataaaat gcttaagttt
      121 tccccaccac ttattagtct ctttaccgat aaaaagtatt tttgtcttag aatttaaata
      181 tttttcttca tctatattta gtaaaaatgg agccgaaatt ttagatattt cagccgtact
      241 gaaatttttt cttaattcat ctttattaaa aatattagaa tacaatttat caagttcatt
      301 ttctatgtgt cccattttta aattttcctt tatactagta tattattacg ttaattctat
      361 ttgattaatg tttttttaaa aattatactc tagccaaaaa gtggttaaaa atttattaaa
      421 aaatcacaat ttaaattatg atttaaataa tcacttaact ataggataat ttcatttcaa
      481 aaaccctact ttcaaactat tatcatcatt tttaaatgat atctcttctt ttatatactc
      541 tagtatttca tcaaaatcaa actctttttg atttagaatt ttatttatta gatattttct
      601 tgttacattc tctatttgtc caccacttaa atcataaatt gatagttttt catagatttt
      661 atcttcaagt tctggtaatt ttgatttcca aatatgctct cttgttttag ctgttggtct
      721 atcaaatttt attttattta aaaatcttct tgaaaaagca tcatccatat tatctattag
      781 atttgttgta gccataaaaa taccatcaaa gttttctaac tcttcaagta aaatattttg
      841 catagtatta ttcatttgag aaactgcatc atttacatct aatcttttag aaatgattgc
      901 atcagcttca ttaaatagca atattggttt tgattttaat atttcacaag ctctataata
      961 ttcttgaaat attgccttag tatttttttc actctcccct acccactttg attgaataga
     1021 gctaatatca acttgcaata catctctttt agtaagtttt gctatctcat aaacagatgc
     1081 tgttttacct gttccagcaa atccataaaa aatagagaca ataccagatg gcaaatttgc
     1141 tttttttaaa tcttttacaa ttttattaaa attctttgag ctacaaacat cttttaattg
     1201 attaatatca cgagcaactc tttcatccaa aaatatctct tttttcaaac tattaaattt
     1261 tatatgtttt gtaaactgtg ctttaaactc ttgtttttta tttttatctt ttgattgaag
     1321 aagcaaagca attgcttttg gtgttaattg tatatcagtt gatgaatcaa aaagaccact
     1381 tctctcttca agttgtacca atttatcttt aaaaatatcc aactcttctt ttaatatact
     1441 ctctaaatat cttgctctat gtgacaaatc atcaaaaaat at
//
LOCUS       DAOQAN010000363         1481 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_135278_length_1481_cov_3.103086, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000363 DAOQAN010000000
VERSION     DAOQAN010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1481
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135278_length_1481_cov_3.103086"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..949
                     /locus_tag="PKO10_05980"
     CDS             <1..949
                     /locus_tag="PKO10_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66730.1"
                     /translation="ILHFKLLYWNTKQYLLYKKSDAYKALIKSNGQISIMTLPLTYTM
                     SINVGFVLGAVFVPNLWSFVEYLFPFALIGFAINGYFALKIFFDYFSRLIINGDFDFT
                     KNNNLTQMISIFALAMVAVGFAAPGAMSSNIAVNAVGIFGAILFSSLAIMLIFIKIIL
                     GFKNMFEHGVTVETAPSLWIMIPILTLLGITFIRLNFGLEHNLNAISDKSSLFVLTST
                     ILSLQIVFGILGLVIMKKLGYFEKFIKSNEKSALSFALICPGVAFFVFGMFFINLGLT
                     YNEIITKYSVVYYLLMVPFIYVQIKTIVLFFRLYKKFSF"
     gene            965..1393
                     /locus_tag="PKO10_05985"
     CDS             965..1393
                     /locus_tag="PKO10_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HNW66731.1"
                     /translation="MKKILLSLSAFALISTTVVASENMAKGLNVMITSEDAQTQMMGM
                     VLATMSLKKGKEVNMVLCSSAGNLALKDSESPKLKPQDKSPKMMLEDLIKNGAKVEVC
                     PLFLPNAEKTEADLIQNVTVAKPPVVADKLLNKDYQNLSY"
BASE COUNT          525 a          185 c          214 g          557 t
ORIGIN      
        1 aattcttcac tttaaacttc tttattggaa tacaaaacaa tacctactat ataaaaaaag
       61 tgatgcttac aaggctttga ttaaatcaaa tggtcaaatt agcattatga cactgccatt
      121 aacatataca atgagtataa atgttggttt tgttttaggt gcagtttttg taccaaattt
      181 atggagtttt gttgagtatc tgtttccatt tgcacttatt ggatttgcta ttaatggata
      241 ttttgctcta aaaatatttt ttgactattt ttcaagattg attattaatg gtgattttga
      301 ttttacaaaa aataacaacc taacacaaat gatatctatt tttgcacttg caatggtagc
      361 agttggattt gcagcacctg gtgctatgag ttcaaatatt gctgtaaatg cagttggaat
      421 atttggagca atacttttct cttcacttgc aattatgttg atatttataa aaataatctt
      481 aggttttaaa aatatgtttg aacatggtgt tacagttgaa acagctccat ctttatggat
      541 tatgattcca atcttaacac ttcttggaat tacatttata agattaaatt ttggacttga
      601 acacaattta aatgcaataa gcgataaatc atcactattt gttttaactt caacaatact
      661 atctttacaa atagtatttg ggattttagg tttggttatt atgaaaaagc ttggatattt
      721 tgaaaagttt ataaaatcaa atgaaaaatc tgcactatct tttgcactaa tttgcccagg
      781 tgttgcattt tttgtatttg gaatgttttt tataaactta ggtcttacat acaatgaaat
      841 tattacaaag tattcagttg tttactattt acttatggtt ccatttatat atgttcaaat
      901 aaaaactata gttctatttt ttagactata caaaaaattc tcattttaaa ataaaggaaa
      961 aaatatgaaa aaaattttat taagtttaag tgcttttgca cttatttcaa caactgttgt
     1021 tgctagtgaa aatatggcaa aaggtttaaa tgtaatgatt acatcagaag atgctcaaac
     1081 tcagatgatg ggtatggttt tagcaactat gagtttgaaa aaaggtaaag aggtaaatat
     1141 ggttttatgt tcaagtgctg gaaatcttgc tttaaaagat agcgaaagcc caaaacttaa
     1201 acctcaagac aaatcaccaa aaatgatgct tgaagattta atcaaaaatg gtgcaaaagt
     1261 tgaagtttgt ccactatttt taccaaatgc tgaaaaaaca gaagctgatt taatccaaaa
     1321 tgttactgtt gctaagccac cagttgttgc tgataaactt ttaaataaag attatcaaaa
     1381 tttaagttat taaaaaccat aaagagattt cattctcttt atgatactaa gtttataaaa
     1441 tcttttatct atttttaact ttaaaactca tattttaaag c
//
LOCUS       DAOQAN010000364         1481 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_135358_length_1481_cov_1.975456, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000364 DAOQAN010000000
VERSION     DAOQAN010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1481
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135358_length_1481_cov_1.975456"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="PKO10_05990"
     CDS             complement(<1..275)
                     /locus_tag="PKO10_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013134560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HNW66732.1"
                     /translation="MDNFILKNENAIYYECNFSCDNVIFLSLGSEKFFITDARYTIEA
                     KEYAKNCEVIESANLIDSAKEILKKNSIKSITFDPNDFTFFEYKNMV"
     gene            353..850
                     /gene="aroQ"
                     /locus_tag="PKO10_05995"
     CDS             353..850
                     /gene="aroQ"
                     /locus_tag="PKO10_05995"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HNW66733.1"
                     /translation="MKIAVIQGPNLNMLGVREQHIYGGVTLEQIHAQLQNAADQNGVE
                     IEFFQSNFEGEIVDRIQECLGTVDGIMINPAAYSHTSIAIRDALAAVNMPVVEVHISN
                     IYKREEFRQKSITAGSSTGVITGFGGFGYHLGLISLIQMLNELKALNDARNAQIQAQT
                     QEENK"
     gene            857..>1481
                     /locus_tag="PKO10_06000"
     CDS             857..>1481
                     /locus_tag="PKO10_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014473343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNW66734.1"
                     /translation="MKILTASYILTFDENFTILKDGCIVFDEKIIELASYDITIKKYP
                     NIEILSLGENSVLMPGLINSHIHLEFSANKTTLKYGSFYSWLNSVIRHREKLIDKAKI
                     SLITQELKKLLKTGTTTIGAISSYSFDLEACLKSPINKIFFCEVIGSKADMIDTLFTD
                     FKARLKDAQKHNSKNFKTGIAIHSPYSVHPFLVREVLNIAKDQNLPVT"
BASE COUNT          557 a          190 c          206 g          528 t
ORIGIN      
        1 tttaccatgt ttttgtactc aaaaaaagta aaatcatttg gatcaaaagt tatagatttg
       61 atactatttt ttttcaaaat ctctttagca ctatcaatta gattagcact ttcaataact
      121 tcacaatttt ttgcatactc ttttgcttca attgtatatc tagcatctgt aataaaaaat
      181 ttctcacttc ctaaacttaa aaaaattaca ttatcacaag aaaagttaca ttcataatat
      241 attgcattct cattttttaa aataaagtta tccactaaga ttgccttttt ttgggttttt
      301 taacaaaaat ttaaatataa tcttacctaa attttataaa aggaatataa taatgaaaat
      361 agcagtaata caaggaccaa acttaaatat gttaggagtt agagagcaac atatttacgg
      421 aggagtaact ttagagcaaa ttcatgctca acttcaaaat gcagctgatc aaaatggagt
      481 tgaaattgag ttttttcaat caaattttga aggagaaata gttgatagaa ttcaagagtg
      541 cttagggact gttgatggaa ttatgataaa tccagctgca tattcgcaca cttcaatagc
      601 aattagagat gctttagcag ctgttaatat gcctgttgtt gaggttcata tttcaaatat
      661 ttataaaaga gaagagttta gacaaaaatc tattacagca ggttctagta caggagtaat
      721 tactggattt ggtgggtttg gttatcattt gggacttatc tctttaattc aaatgttaaa
      781 tgaattaaaa gctttaaatg atgcaagaaa tgctcaaata caagcacaaa cacaagaaga
      841 gaataaataa gcttttatga aaatattaac tgcttcttat atccttacat ttgatgaaaa
      901 ctttactatt ttaaaagatg gttgtattgt ttttgatgaa aaaattattg agcttgcaag
      961 ttatgatatt actataaaaa aatatccaaa tattgaaatt ttaagtcttg gagaaaactc
     1021 tgttttaatg ccaggactta taaactcgca tattcatctt gaatttagtg caaataaaac
     1081 aactttaaaa tatggtagtt tttactcttg gttaaattct gttataagac atagagaaaa
     1141 acttattgat aaagctaaaa tatctttgat aactcaagag ttaaaaaaac ttttaaaaac
     1201 aggaactaca actataggtg ctatatcatc ttactctttt gatttagaag cttgtttaaa
     1261 atccccaatt aataaaatct ttttttgtga agtaattggt tcaaaagcag atatgattga
     1321 tactttattt actgatttta aagcaagatt aaaagatgct caaaagcata atagtaaaaa
     1381 ttttaaaaca ggtattgcaa tacattcgcc atactctgtt catccatttt tagttcgtga
     1441 agttttaaat attgctaaag atcaaaatct tccagttact t
//
LOCUS       DAOQAN010000365         1480 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_135491_length_1480_cov_2.144561, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000365 DAOQAN010000000
VERSION     DAOQAN010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135491_length_1480_cov_2.144561"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..944)
                     /locus_tag="PKO10_06005"
     CDS             complement(<1..944)
                     /locus_tag="PKO10_06005"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HNW66735.1"
                     /translation="MFSYENLKKILFIFEPEKAHNIAESGLKLLGKCKLLKNYYEKKN
                     FISDERLHQKIFNVKFENPVGLAAGFDKNATMVKAMKSMGFGFTEIGTTTPMPQDGNP
                     KPRMFRYPEYKSVQNAMGFNNLGAHAVLKNLKKVYPFSIPIGVNIGKNKTTPEEYALN
                     DYKTLIKKLESYGDYIVINISSPNTPNLRDLQNEKFINELFSMAKTLTNKPILLKIAP
                     DMEVQTAIELCKTAINSGAAGIIATNTTIDYSLVPNCKDFGGLSGACLSEKSSLLFKE
                     IAGELYGKTILISVGGISNANQAYERIKNGASLVQAYSG"
     gene            complement(990..>1480)
                     /locus_tag="PKO10_06010"
     CDS             complement(990..>1480)
                     /locus_tag="PKO10_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNW66736.1"
                     /translation="NCGAVLQETFIFNDTIAANVSYGEELDDLKVIEALKQAHAYEFV
                     LSMKKGINTVLDEFGTNLSGGQRQRIAIARALYKNPKILILDEATSALDNKSESLISQ
                     VIEEVSKDRITFVIAHRLSTIRNASKIALFKNGEIVDIGTQEELLKNSTEYQRLYNSA
                     NI"
BASE COUNT          468 a          246 c          165 g          601 t
ORIGIN      
        1 cctgaatacg cttgaactaa actcgctcca ttttttattc tttcataggc ttgatttgca
       61 tttgaaattc caccaacact tattaatata gtttttccat aaagctcacc agcaatctct
      121 ttaaataaaa gtgaagattt ttcactaagg caagcaccac ttaatcctcc aaaatcttta
      181 caatttggaa ctaaactata atcaatagtt gtgtttgttg caataattcc agctgctcca
      241 ctatttatag cagttttaca taactctatt gctgtttgta cttccatatc aggtgcaatt
      301 tttaataaaa ttggtttatt cgtaagagtt tttgccatag agaaaagttc atttataaac
      361 ttctcatttt gtaaatctct taaatttggt gtatttggac ttgatatatt tattacaata
      421 taatcaccat aagattctaa ctttttaatt aaagttttat agtcatttaa agcatactct
      481 tctggagttg ttttattttt tccaatattt accccaatag gtatagaaaa agggtaaact
      541 ttttttagat tttttaaaac tgcgtgagct cctaaattat tgaatcccat tgcattttga
      601 acagatttat attcaggata tctaaacatt ctaggttttg gatttccatc ttgtggcata
      661 ggagtagttg ttccaatctc tgtaaatcca aatcccatac ttttcatagc tttaaccatt
      721 gttgcatttt tatcaaatcc agctgcaaga cctacaggat tttcaaattt tacattaaaa
      781 attttttgat gaagtctttc atcacttata aaattctttt tttcataata gttttttagt
      841 aacttacatt ttccaagaag ttttaaacca gattcagcaa tattatgagc tttttcaggt
      901 tcaaatataa atagtatttt ttttagattt tcataactaa acaaagactt tccttttttt
      961 aaaaattcaa atatattagc aaaaaataat caaatatttg ctgaattata caatctttgg
     1021 tactctgtgc tattttttaa aagctcctct tgagttccaa tatctacaat ttccccattt
     1081 ttaaagagag caatttttga agcatttctt atagtgctta atctgtgagc tattacaaaa
     1141 gtaattctat ctttactaac ttcttctatt acttgactta ttaaagattc acttttgtta
     1201 tctaatgcag atgttgcttc atctaaaatt aatatttttg gatttttata taaagctctt
     1261 gctattgcaa ttctttgtct ttgaccaccg cttaaatttg ttccaaattc atctaaaact
     1321 gtgttaatac cttttttcat acttaaaaca aactcataag catgtgcttg ttttaaagct
     1381 tctataactt ttaaatcatc aagttcttca ccataagata catttgctgc aattgtatca
     1441 ttaaaaataa acgtttcttg taatactgct ccacaattca
//
LOCUS       DAOQAN010000366         1478 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_135763_length_1478_cov_2.268447, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000366 DAOQAN010000000
VERSION     DAOQAN010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1478
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135763_length_1478_cov_2.268447"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="PKO10_06015"
     CDS             complement(<1..218)
                     /locus_tag="PKO10_06015"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HNW66737.1"
                     /translation="MSFDEYKKKFDLIQDEIKNGNSYLLNLTAQTKIDTNLTLDNIYE
                     ASSSLLKLRVKLDDLDFVCFSPEKFIDI"
     gene            complement(408..1292)
                     /locus_tag="PKO10_06020"
     CDS             complement(408..1292)
                     /locus_tag="PKO10_06020"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HNW66738.1"
                     /translation="MRHLIRTSDFTKEEILKIFDDAREFKNNKFSKLLEGKIIVTLFF
                     ENSTRTRSSFEIAAKRLGAEVVNLDVGTSSQKKGETMYDTVSNINAMNPDAIIIRHSV
                     HGLPESLIGYVDCPIINAGDGRHSHPTQAFLDLFTINEYFGGKTEGKKIAIVGDVRNS
                     RVAGSNRRLLPRFGIDVNLVAPDCFKYEGDEFKQFDTIQEVIDDMDIIMSLRSQLERH
                     NITYFESLQEYAKDFCITSELMERKEFLLLHPGPVNRNIDITDEVLKHPRCKVLEQVT
                     NGVAIRAAILKKLILEDR"
BASE COUNT          479 a          233 c          171 g          595 t
ORIGIN      
        1 tttatatcta taaatttttc tgggctaaag cagacaaaat ctaaatcatc caactttact
       61 cttaatttta ataaagagct tgatgcttca taaatattat ctaaagttag gtttgtatct
      121 atttttgttt gcgcagttaa atttagtaaa tagctattcc cattttttat ctcatcttga
      181 attaaatcaa actttttttt gtactcatca aaactaattg gatatttttt taaactgtaa
      241 tcttttatat tttttttatt tttactatct atttcaaatt ttatattttg tgagagattt
      301 tgaagttttt ctatatagat tttttctaaa tcataagaga ttaaaaataa aaaaggctct
      361 tttaaagagc caaatttatt tagaatatct tgaaagttag taactattta tctatcctct
      421 aggattaatt tttttaatat agcagctcta atagcaactc cgtttgttac ttgctctaaa
      481 actttacatc ttggatgttt taaaacttca tcagtaatat caatatttct attaactgga
      541 cctggatgaa gaagtaaaaa ctcttttctt tccatcaact cacttgtaat gcaaaaatct
      601 tttgcatact cttgaagtga ctcaaaatat gtgatattgt gacgttcaag ttgacttctt
      661 aaactcataa taatatccat atcatctatt acttcttgaa ttgtatcaaa ctgtttaaac
      721 tcatcacctt catatttaaa acaatcagga gctactaaat ttacatctat tccaaatcta
      781 ggaagtaatc ttctattact tccagcaacc cttgaatttc taacatctcc aacaattgct
      841 atttttttac cttcagtttt tccaccaaaa tattcattaa ttgtaaaaag gtctaaaaaa
      901 gcttgagttg gatgtgagtg tcttccatca cctgcattta ttatagggca atcaacataa
      961 cctatcaaac tctctggaag accgtgaaca ctatgtctaa taattatagc atcagggttc
     1021 atagcattta tatttgaaac agtatcgtac attgtttcac cttttttttg agaagaggta
     1081 ccaacatcaa gatttacaac ttcagctcct aatctttttg cagctatttc aaaagagctt
     1141 cttgttcttg ttgagttttc aaaaaatagt gtaacaatga ttttaccctc taagagtttg
     1201 ctaaatttat tattcttaaa ctctctagca tcatcaaaaa ttttgagtat ctcttctttt
     1261 gtaaagtccg aagtacgaat taggtgtctc atttttttcc tttgatttta gtttcgaatt
     1321 ctagcaaaaa gcactttaaa aataaacttc ttgtataatc taagaatttt taaggtaaca
     1381 aggaaaattt tatgaagctt aaactcttta tagtattaat tttttcattt ttattttttg
     1441 gatgttcaag taaaccactt gatccagtaa gttttgat
//
LOCUS       DAOQAN010000367         1474 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_136267_length_1474_cov_2.999295, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000367 DAOQAN010000000
VERSION     DAOQAN010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1474
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136267_length_1474_cov_2.999295"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..788)
                     /locus_tag="PKO10_06025"
     CDS             complement(<1..788)
                     /locus_tag="PKO10_06025"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNW66739.1"
                     /translation="MQEQKKFISNIHPFNNLNTFELDDLVEDLDIVYFKANSIVQAQD
                     SNPEFLYFVLKGLIQEINDDEVLSVYSKGEIFDSVSLIKNHSKNSFVAIEESICYALK
                     KERFMQILSSNQQLENYFFQSISDKLNNNILNEKNKDMANIMIAKVKDAKVHKAVVVD
                     TNKTIYEAAKIIKQEKIPTLLLKDEDGEMYIVTDSDFRQKVILNRMDYDDFVVKIASK
                     GLIYINEDDFLFNAQLQMAKHGLKRVVVKNDHDEIVGILDQISLS"
     gene            complement(855..>1474)
                     /gene="actP"
                     /locus_tag="PKO10_06030"
     CDS             complement(855..>1474)
                     /gene="actP"
                     /locus_tag="PKO10_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012308.1"
                     /note="member of the sodium:solute symporter family;
                     cotranscribed with the acs gene which encodes acetyl
                     coenzyme A synthase; mutations affect acetate uptake;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HNW66740.1"
                     /translation="VHLSHMLGGNAFLGFISAVAFATILAVVSGLTLAGASAISHDIY
                     ANVINPNASDEKVVKISKITVIIVGIVGVTLGIAFESQNIAYMVGLAFGIAASANFPI
                     LFLSIYWSKLTTRGAFIGGFMGLITAVSLVILGPNVWVQILGNEKAIFPYAHPALFSV
                     TVAFVSIWFFSIIDNSKRATTERAMFRAQNVRANTGIGSAEAVSH"
BASE COUNT          512 a          250 c          184 g          528 t
ORIGIN      
        1 gaaagagata tttgatcaag aattcctaca atctcatcat ggtcattttt tacaactact
       61 ctttttaatc catgttttgc catttgaagt tgagcattaa ataaaaaatc atcttcattt
      121 atataaatta agcccttaga agcaattttt acaacgaaat catcataatc cattctattt
      181 aaaataactt tttgtctaaa atctgaatca gttacaatat acatttcacc atcttcatct
      241 tttaataata gggttggaat tttttcttgt tttataatct ttgctgcttc ataaattgtt
      301 ttgtttgtat caacaactac agctttatga acttttgcat ctttaacttt ggcaatcata
      361 atatttgcca tatctttatt tttttcattt aagatattat tgtttaactt atctgaaatg
      421 gattgaaaaa aatagttttc tagttgttga tttgagctta aaatctgcat aaatctctct
      481 tttttaagag cgtaacaaat actctcttcg atagccacaa aactattttt actatgattt
      541 tttatcaaag atacagaatc aaatatctca ccttttgaat atacacttaa aacttcatca
      601 tcgtttatct cttgaattaa accttttaaa acaaaatata aaaactctgg attactatct
      661 tgtgcttgaa ctattgagtt tgctttaaaa tagacaatat ctaaatcttc gactaaatcg
      721 tccaactcaa atgtatttaa attattaaat gggtgaatat ttgaaataaa ttttttttgc
      781 tcttgcatac tcatctctcc tatatttaaa gtagtgcaaa atcaaaaaga gaagagattc
      841 tctttttgat atttttagtg tgaaactgct tctgctgaac caattccagt gttagctcta
      901 acattttgag ctctaaacat agctctctct gtagtagctc tttttgagtt atcaattata
      961 gagaagaacc aaattgaaac aaacgcaact gtaacagaga atagcgcagg gtgtgcataa
     1021 gggaaaattg ctttttcatt tcctaaaatt tgaacccaaa catttgggcc taaaattact
     1081 agagaaactg ctgtaattaa tcccataaat ccaccaataa atgcacctct tgttgttaat
     1141 tttgaccaat aaattgataa aaacaaaatt gggaagttag cacttgcagc gattccaaat
     1201 gcaagtccaa ccatatatgc aatattttga gactcaaatg caattccaag tgtaactcca
     1261 acaataccta caattataac agttattttt gaaattttta caactttttc atcacttgca
     1321 tttggattta taacattcgc ataaatatcg tgtgaaattg cactagcacc tgctagagtt
     1381 agtccagaaa caacagccaa aattgtagca aatgcaacag ccgaaataaa tcctaagaaa
     1441 gcatttccac caagcatatg agataggtga actg
//
LOCUS       DAOQAN010000368         1469 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_136994_length_1469_cov_2.410891, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000368 DAOQAN010000000
VERSION     DAOQAN010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1469
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136994_length_1469_cov_2.410891"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="PKO10_06035"
     CDS             <1..203
                     /locus_tag="PKO10_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014475546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HNW66741.1"
                     /translation="AVVKNGEKKSGVTIHYVNENYDEGEKILIKELFLDENETAETLE
                     NRIKELEKIAIVEAFKKVLEER"
     gene            complement(221..682)
                     /locus_tag="PKO10_06040"
     CDS             complement(221..682)
                     /locus_tag="PKO10_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012148153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66742.1"
                     /translation="MLKKILLASLLALGLNAAPLAVDSVVDNLKIKDQNEVEKVIDTN
                     VKTILFASDKSTSDMLRDYLLPLSEKENILEKNSAIYVADISGMPSLISKFIALPKMK
                     KYPFSVLLLDDTNKDNFVKEDGKIIVYSLENGKVVKIDKISTKEELAKFIK"
     gene            complement(736..1353)
                     /locus_tag="PKO10_06045"
     CDS             complement(736..1353)
                     /locus_tag="PKO10_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HNW66743.1"
                     /translation="MSALEIADIIGIISFALSGFLIAVHCKLDILGVFISAFLTAFGG
                     GMTRDVLADRTPYVFTSNLPLSLVIATVLIAMLFKLHKITNLEGKWAFVISDAIGLSS
                     FAITGAIIAIESDFNFLSAVMLAFITAVGGGTIRDVLINRMPFILVSEFYATVALIIG
                     SVVYILDAFELRNIFSLIIVFIFGVVLRVLAYYKKWHLPTLSKEN"
     gene            complement(1350..1451)
                     /locus_tag="PKO10_06050"
     CDS             complement(1350..1451)
                     /locus_tag="PKO10_06050"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HNW66744.1"
                     /translation="MPKQLDNYFKLRLDEHAQWEIRQTAIAMQELLR"
BASE COUNT          559 a          226 c          190 g          494 t
ORIGIN      
        1 aagctgtagt aaaaaatggt gagaaaaaat caggtgttac aattcattat gtaaatgaga
       61 attatgatga gggtgagaaa attttaataa aagagctatt tttggatgaa aatgaaacgg
      121 cagaaacttt ggaaaataga ataaaagagc tagaaaaaat tgctattgta gaagcattta
      181 agaaggtttt agaggagaga taaaacttct cctctagtgt ttatttaata aatttagcta
      241 actcttcttt tgtagagatt ttatctattt ttacaacttt tccattttct aaactatata
      301 caataatttt tccatcttct tttacaaagt tatctttgtt tgtatcatct aataataaaa
      361 ctgaaaatgg gtattttttc attttaggta aagctataaa tttagatatt aaacttggca
      421 ttcctgaaat atcagcaaca taaatagcac tatttttttc taaaatattc tctttttcac
      481 ttagaggtaa aagataatct cttaacatat cgcttgtact tttatcactt gcaaaaagta
      541 tagtttttac atttgtatcg ataacttttt caacttcatt ttgatcttta atttttaagt
      601 tatcaacaac actatcaaca gcaagtggag ctgcatttaa tcctaaagct aataaagaag
      661 ctaaaagtat tttttttaac atatttttcc ttttaaaaat tttcaagatt ctagctttaa
      721 aaagtaaaca gaagattaat tttcttttga taaagttggt aaatgccact ttttatagta
      781 tgcaagaact cttaaaacaa ctccaaatat aaatacaata atcaaagaga atatatttct
      841 aagttcaaat gcatctaaaa tataaacaac acttcctata attaaagcaa cagttgcata
      901 aaactctgaa actaaaataa aaggcattct atttattaaa acatctctta tagttcctcc
      961 accaacagct gttataaatg caagcattac agcacttaag aagttaaaat cgctctcaat
     1021 tgcaattatt gcacctgtta ttgcaaatga tgaaagtcca attgcatcag agattacaaa
     1081 agcccattta ccctctaaat tcgtaatttt atgaagttta aaaagcattg caattaaaac
     1141 agttgcaatt actaaactaa gagggaggtt tgaagtaaaa acgtaaggag ttctatctgc
     1201 taaaacatct cttgtcatcc caccaccaaa agcagttaaa aacgcagata taaaaactcc
     1261 tagaatatca agcttacagt gaactgctat taaaaaacca cttagggcaa aagagataat
     1321 accaataata tcagcaatct ctaaagcact catcttaaaa gctcttgcat tgcaattgca
     1381 gtttgtctaa tctcccattg agcatgttca tcaagtctta acttgaaata attatcaagc
     1441 tgttttggca taaaggcacc ccaaatttg
//
LOCUS       DAOQAN010000369         1469 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_137016_length_1469_cov_2.173267, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000369 DAOQAN010000000
VERSION     DAOQAN010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1469
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137016_length_1469_cov_2.173267"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="PKO10_06055"
     CDS             <1..352
                     /locus_tag="PKO10_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013327828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNW66745.1"
                     /translation="YDNFGFKYGSYIPDINKALENCKNSPTVLLAHQPKFINSISNTS
                     NIDLILCGHTHGGQIFPFNFLVKLDQPYVKGLNIHNESTQVYVNKGTGFWGPPMRLGA
                     SSEITILNLKEAKA"
     gene            complement(364..780)
                     /locus_tag="PKO10_06060"
     CDS             complement(364..780)
                     /locus_tag="PKO10_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNW66746.1"
                     /translation="MLLTKFDPFKQLKEIEKNLYTQVGNNEGVTAFVPTVNTREGEFA
                     YHVDVDLPGVKKEDIKVDLNKGVLTISGERKTKEEVKQEDYYKIETYFGKFSRSFTLP
                     DNVDIENIEAKSDNGVLEIVIPKLKDDVSKKSIEIK"
     gene            complement(883..1326)
                     /locus_tag="PKO10_06065"
     CDS             complement(883..1326)
                     /locus_tag="PKO10_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin family protein"
                     /protein_id="HNW66747.1"
                     /translation="MLIDKNNLPIVDMDFMNETHYEDVDLINDLYKNIELYNQDSSLE
                     NFENLKMKYKEWIEHTVNHFATEEEEMVKRGFFAYPFHKGEHDANIEEIKAVWNNFEK
                     SKDILKLKNYIEYDVINWLINHIKSMDTVTARFFKTGMMGGCGMM"
BASE COUNT          463 a          252 c          147 g          607 t
ORIGIN      
        1 ttatgataac tttggtttta aatatggctc atatattcca gatattaata aagctttaga
       61 gaattgtaaa aactctccaa cagttctttt agctcatcaa ccaaaattta ttaactcgat
      121 ttccaataca tcaaatattg atctgatttt atgtggacac actcatggtg gtcagatttt
      181 tccatttaat ttcttagtaa aacttgatca accttatgta aaaggtttaa atatacacaa
      241 tgaatcaact caagtatatg taaacaaagg aacaggattt tggggaccac caatgagact
      301 tggtgctagt agtgagataa ctattttaaa tctaaaagag gcaaaagcct aagcttttac
      361 ctcttattta atctctattg attttttcga tacatcatct ttaagtttag gaataactat
      421 ttctaaaact ccattatcac tttttgcttc tatattttca atatctacat tatctggaag
      481 tgtgaaactt cttgaaaatt taccaaaata tgtctctatt ttgtaataat cttcttgttt
      541 tacctcttct ttagtttttc tttcaccact tatagttaag acacctttgt ttaaatcaac
      601 ttttatatct tcttttttta cacctggcaa atcgacatca acatgatatg caaactcacc
      661 ttctcttgta tttacagttg gtacaaaagc tgtaacacct tcattatttc caacttgtgt
      721 atataaattt ttctctatct cttttaactg tttaaatggg tcaaattttg ttaataacat
      781 tgtgaactcc tattaaaata ttaagcttga tagtacttct atcaaactgc tgaaatttta
      841 tcaaattttt tggcacttgt caagatagag tgctaattta tattacatca taccacaacc
      901 acccatcatt ccagttttga aaaatcttgc agttactgta tccatacttt ttatatgatt
      961 tataagccaa tttattacat catattcaat atagttttta agtttcaata tatctttgct
     1021 tttctcaaaa ttattccaaa ctgcttttat ctcctctata tttgcatcat gttcaccttt
     1081 atgaaatgga tatgcaaaaa aacctctttt taccatctcc tcttcttccg ttgcaaaatg
     1141 gtttacagta tgttcaatcc actctttata tttcattttt agattttcaa aattttctaa
     1201 agaactatct tgattataaa gttctatatt tttataaaga tcatttatta gatcaacatc
     1261 ctcatagtga gtctcattca taaaatccat atcaacaatt ggtaagttat ttttatctat
     1321 taacatttta aatcctaaaa aatttttgtg atagtaacta tttacatatt attttatgtt
     1381 gattatagtc aattatattc cagctccatc ttcaaaactt cttaattttc ttgttctaat
     1441 tcctactata tcaccttttt ttttttttt
//
LOCUS       DAOQAN010000370         1458 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_138562_length_1458_cov_3.053457, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000370 DAOQAN010000000
VERSION     DAOQAN010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1458
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138562_length_1458_cov_3.053457"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="PKO10_06070"
     CDS             <1..199
                     /locus_tag="PKO10_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HNW66748.1"
                     /translation="IFVLETVSVMLQVGSYKLRQKRVFLMAPIHHHFEQKGWKENKII
                     VRFWIISFMANLVALLSLKLR"
     gene            202..1362
                     /gene="murD"
                     /locus_tag="PKO10_06075"
     CDS             202..1362
                     /gene="murD"
                     /locus_tag="PKO10_06075"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008337836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HNW66749.1"
                     /translation="MQNLRILGKGKTALALKKRFPDATLYDDNDLEYFDKNSNDLTIV
                     SPGMPPHNKLVLSSKNIISDYDLFYDEMPFTIWISGTNGKTTTTQMCQYLLEKFDSCY
                     GGNIGVPLSGLNKDKKIWILETSSFTLHYTNKAKPNIYILLPITEDHITWHGSFDEYK
                     KAKLKPLELMNETDIAIIPALYKDFKTSAYTIYYNSSDDLCNHFKINKSKIKFNEPFL
                     LDAIMAMVSRKIIFGDVDYDLINSYKIDKHKVEEFRDIKNRLWIDDSKATNYDATINA
                     LVPYKDKNIHIIIGGDDKGASLKPLFENIKNLDIVIYAIGSNTQRVLNYCEEFKISCV
                     KCEYLEEAVKNIDKNLKENSIGILSPAAASLDQFKSYAHRGDEFKKFVRNLS"
BASE COUNT          571 a          162 c          230 g          495 t
ORIGIN      
        1 tatctttgtt ttagagacgg tttcagttat gttacaagtt ggttcatata aattaagaca
       61 aaaaagagtt tttttaatgg ctcctattca tcaccatttt gaacaaaaag gttggaaaga
      121 gaataaaata attgtgcgat tttggataat atcttttatg gcaaatcttg ttgcactatt
      181 aagtttaaag ttgagataaa aatgcaaaat ttaagaatct taggaaaagg taaaacagca
      241 cttgctttga aaaaaagatt tccagatgct actttatatg atgataatga tttagaatat
      301 tttgataaaa attcaaatga tttaactatt gtaagccctg gaatgcctcc acataataaa
      361 cttgttcttt cttcaaaaaa tattattagt gattatgatc tattttatga tgaaatgcct
      421 tttactattt ggattagtgg aacaaatgga aaaactacaa caacacaaat gtgtcaatat
      481 ctgcttgaga aatttgattc ttgttatggt ggaaatattg gtgttccttt gagtggatta
      541 aataaagata agaaaatttg gatacttgaa acatcatcat ttacacttca ctatacaaat
      601 aaagcaaaac caaatatcta tattctttta ccaataacag aagatcatat aacttggcat
      661 ggaagttttg atgaatataa aaaagcaaaa ttaaaacctt tagagcttat gaatgaaaca
      721 gatattgcta ttatcccagc tttatataaa gatttcaaaa catctgcata tacaatttat
      781 tataattcaa gtgatgattt atgtaatcat tttaaaatca ataagagtaa aataaagttc
      841 aatgaaccat tcttacttga tgctataatg gcaatggttt caagaaagat aatttttggt
      901 gatgtagatt atgatttaat aaactcttat aagattgata aacacaaagt tgaagagttt
      961 agagatatta aaaatagatt atggattgat gatagtaaag ctacaaatta tgatgcaaca
     1021 ataaatgcat tagttccata taaagataaa aacatacata ttattattgg tggtgatgat
     1081 aagggtgcaa gcttaaaacc tctttttgaa aatataaaaa atttagatat tgtgatttat
     1141 gcaataggta gtaatactca aagagtttta aattactgcg aagagtttaa aatatcttgt
     1201 gttaagtgtg aatatttaga agaagcagtt aaaaatatag ataaaaatct aaaagaaaat
     1261 agtataggaa tactttctcc agctgcagca tctcttgatc aatttaaatc ttatgctcat
     1321 agaggagatg aatttaaaaa gtttgtgaga aatttaagtt aatattaata aattcaactg
     1381 tataatctca ctccaatttg aagtggctcg atagctcagt cggtagagca aaggattgaa
     1441 aatccttgtg tcgacagt
//
LOCUS       DAOQAN010000371         1454 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_139207_length_1454_cov_2.443888, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000371 DAOQAN010000000
VERSION     DAOQAN010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139207_length_1454_cov_2.443888"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="PKO10_06080"
     CDS             <1..396
                     /locus_tag="PKO10_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HNW66750.1"
                     /translation="KNIDVVVLNRSKEKLGFFKNNGIKAFSFEDFDFKNEKFDLVVNS
                     TSAGLKDDFLPAPKEILENILSKSSFAFDCIYGKITPFLALAKNLNNKIKDGEDMLLF
                     QGVLAFELFTKTKADERLVEAMRRGLKGE"
     gene            complement(418..1014)
                     /locus_tag="PKO10_06085"
     CDS             complement(418..1014)
                     /locus_tag="PKO10_06085"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNW66751.1"
                     /translation="MKKISLYTNLKRDYEKMKNSLIDYDLKNIENREELLTLKENLII
                     INTSVNLDKEEETIEILLENRNKILILESTPKLINAQNWLLLGVHGYGNSMMNDIYLK
                     SAIQTINNGLTWLIPDITMEILSKLNSDSLNQKDVLNQLSKAEQQVAILLKDGLSNTK
                     ISEELKLSINTVKSHIKNIYLKLDVKDRFSFINLLNKK"
     gene            complement(1041..>1454)
                     /locus_tag="PKO10_06090"
     CDS             complement(1041..>1454)
                     /locus_tag="PKO10_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNW66752.1"
                     /translation="HINTVGGSIKPAQDLLEIVPTDSNLIVEVKILPKDIAFIYQGQK
                     AIVKFSAFDFSIFGGLDGHVINISPDTIVEKDEKTFYIVRIETEKNYIGDEKNQKHII
                     PGMIADVDILTGKKTVLDYILKPILKTKTYTFTER"
BASE COUNT          501 a          189 c          165 g          599 t
ORIGIN      
        1 aaaaatattg atgtagtagt tctaaataga agcaaagaaa aactaggatt ttttaaaaat
       61 aatggtataa aagcttttag ttttgaagat tttgatttta aaaatgaaaa gtttgattta
      121 gttgtaaact caacaagtgc tggtttaaaa gatgattttt taccagctcc taaagagata
      181 cttgaaaata ttttaagtaa gtcatctttt gcttttgatt gtatttatgg gaaaataact
      241 ccatttttag ccttggcaaa aaatttaaat aataaaataa aagatggcga agatatgctt
      301 ttatttcaag gtgtccttgc ctttgaactt tttaccaaaa caaaagccga tgagagatta
      361 gttgaagcta tgagaagagg tttaaaaggt gaataaaaag tctataaaat agacttttta
      421 ctttttattt aaaagattta taaatgaaaa tctatctttt acatcaagct ttaaatagat
      481 attttttata tgcgatttta ctgtatttat agataatttt aactcttcac taatttttgt
      541 atttgatagc ccatctttta ataatattgc tacttgctgc tcagctttag agagttgatt
      601 taaaacatct ttttgattta aactatcact atttaattta cttaaaattt ccattgttat
      661 atctggaatt aaccaagtta aaccattgtt tattgtttga attgctgatt ttaaataaat
      721 atcattcatc atactatttc cataaccatg aacacctaaa agtaaccaat tttgtgcatt
      781 tataagtttt ggagtactct ctagtattaa aattttattt ctattttcta acaatatttc
      841 aatagtttct tcttctttat caagatttac agaagtattt ataataataa gattctcttt
      901 taaagtaagt aactcttccc tattttctat attttttaaa tcataatcaa taagactatt
      961 tttcattttt tcatagtctc tttttagatt tgtatataaa gaaatttttt tcatactcaa
     1021 atacccccta ttttttaatg ttatctctct gtaaatgtat atgttttcgt ttttaagatt
     1081 ggttttaaaa tataatcaag aactgttttt ttaccagtta aaatatcaac atctgctatc
     1141 atacctggaa taatatgttt ttgatttttc tcatcaccta tatagttctt ctctgtttca
     1201 attcttacaa tataaaaagt tttttcatct ttttcaacta ttgtatctgg gcttatattt
     1261 ataacatgac catcaagtcc accaaaaata gagaaatcaa atgctgaaaa tttaacaata
     1321 gctttttgcc cttgatatat aaatgctata tcttttggta aaatctttac ttcaactatt
     1381 aaattactat cagtaggaac tatttctaat aaatcttgag caggtttaat acttccacca
     1441 acagtattta tatg
//
LOCUS       DAOQAN010000372         1452 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_139521_length_1452_cov_2.400143, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000372 DAOQAN010000000
VERSION     DAOQAN010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1452
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139521_length_1452_cov_2.400143"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1039
                     /locus_tag="PKO10_06095"
     CDS             <1..1039
                     /locus_tag="PKO10_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HNW66753.1"
                     /translation="NINSPKQLGVILFEKLGLVSSKKTKTGYSTDESVLESLKNEHKV
                     VPLLLKYREAFKLHSTYIEPLLDLGLKDSENRVFSSFLHTGTTTGRLSSKNPNLQNIP
                     VGTFSDIQIRRAFIAQEGYKLVGVDYSQIELRLLAHFSQDKALVEAFRNDLDIHLQTA
                     IKIFGEDEAKDKRAIAKTINFGLLYGMGSKKLSDTLGISTKEAKTYIDSYFEAFKSVK
                     DYFKSIEDEAIEKGYVKTLLNRKRFFDFQGATPMLKAAYLREAVNTLFQGSAADLIKL
                     SMINIHLKYKDNPNVKMLLQIHDELIFEVKDEYVDEVIEDIKEIMESIFVLNVPLKVS
                     VGVANSWQDLK"
     gene            1082..1435
                     /locus_tag="PKO10_06100"
     CDS             1082..1435
                     /locus_tag="PKO10_06100"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66754.1"
                     /translation="MIFYLLAVVLGFALLVWSADRFVDGAASTAKHLGMPSLLIGILI
                     VGFGTSAPEMVVSAIAAYEGNPGLALGNAIGSNIVNIALILGVTAIVSPIMVNSKIVK
                     KEIPLFLLIVLITKY"
BASE COUNT          528 a          168 c          258 g          498 t
ORIGIN      
        1 taatataaac tctccaaagc agttgggtgt tattttgttt gaaaaactag gacttgtatc
       61 ttcaaagaag acaaaaacag gttatagtac agatgagtct gttttggaga gcttaaaaaa
      121 tgagcataaa gtagttcctc ttttattaaa atatagagag gcttttaaac ttcactcaac
      181 atatattgaa ccacttttag atcttggatt aaaagatagt gaaaatagag ttttctcttc
      241 atttttacat acaggtacta caacaggaag attaagctca aaaaatccaa atttacaaaa
      301 tattccagtt gggactttta gtgatattca aattagaaga gcttttatag cacaagaggg
      361 ttataagctt gttggagttg attattcaca aattgagctt aggcttcttg cgcatttctc
      421 acaagataaa gctttagttg aagcttttag aaatgattta gatattcact tacaaactgc
      481 tataaaaatt tttggtgaag atgaagcaaa agataaaaga gctattgcaa aaactataaa
      541 ctttgggctt ttatatggaa tgggaagtaa aaaactatca gatactttag gaatttctac
      601 aaaagaggca aaaacatata ttgattcata ctttgaagca tttaaaagtg taaaagatta
      661 ttttaaatct attgaagatg aggctattga aaagggttat gtaaaaacac tattaaatag
      721 aaaaaggttt tttgattttc aaggtgcaac tcctatgtta aaagccgcat atttaagaga
      781 ggctgtaaat acgctatttc aaggaagtgc agctgattta ataaaactat ctatgataaa
      841 tatccatcta aaatataaag ataatccaaa tgtaaaaatg cttttacaaa tacacgatga
      901 gcttattttt gaagttaaag atgagtatgt tgatgaagtt atagaagata taaaagagat
      961 tatggagagt atttttgtat taaatgttcc tttaaaagta tctgttggtg ttgctaactc
     1021 ttggcaagat ttaaaataat taaaagtata cttccgaaaa tttattaagg aagatatatt
     1081 tatgatattt tatctactag cagtagtatt aggatttgct cttttagttt ggagtgcaga
     1141 taggtttgta gatggtgctg catcaacagc aaagcatctt ggaatgccaa gtttattaat
     1201 aggtatttta attgttggat ttggaacaag tgctcctgag atggttgttt ctgcaatagc
     1261 tgcttatgaa ggaaatccag ggcttgcact tggaaatgct attggatcaa atattgtaaa
     1321 tattgcattg attttaggag ttacagctat tgtctctcca attatggtta attcaaaaat
     1381 agttaaaaaa gagattccac tttttcttct aatcgtattg attacaaaat attaacatat
     1441 ggataatatt tt
//
LOCUS       DAOQAN010000373         1445 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_140685_length_1445_cov_1.803597, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000373 DAOQAN010000000
VERSION     DAOQAN010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140685_length_1445_cov_1.803597"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..898)
                     /gene="hslU"
                     /locus_tag="PKO10_06105"
     CDS             complement(<1..898)
                     /gene="hslU"
                     /locus_tag="PKO10_06105"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HNW66755.1"
                     /translation="MDMTPRQIVAYLDDYIIGQKDAKKTIALALRNRYRRMRVEPNLQ
                     EEIMPKNILMIGSTGVGKTEIARRMAKMMGLPFVKVEASKYTEVGFVGRDVESMIRDL
                     LYESINLVTKEYEEKIKDKIDDEVNKKIIEKLVPPLPDSASENAKESFIKTYNLMEKK
                     LLNGDLDDKKIEIELPKKAHVEIIDSNLPFDMSSMQESLNKMLGSLNKEKIKKEVSIK
                     DAKILLRGVASEGLLDTEAIKIEALKRAENGGIIFIDEIDKIASGKKNQGQDPSKEGV
                     QRDLLPIVEGSSVQTKFGQIKTD"
     gene            complement(906..1442)
                     /gene="hslV"
                     /locus_tag="PKO10_06110"
     CDS             complement(906..1442)
                     /gene="hslV"
                     /locus_tag="PKO10_06110"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014473935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HNW66756.1"
                     /translation="MFHATTILAYKGKNKSVIGGDGQVSFGNTVLKGNATKIRTLYKG
                     QILAGFAGSTADAFNLFDMFEAHLETCKGDLLKSVIAFSKEWRKDKYLRRLEAMMIVL
                     NKEKIFILSGNGDVVEPEDGEIASIGSGGNFAISAARALAKHSQLDEEELVRESLMIA
                     GELCIYTNQNIKILKIEE"
BASE COUNT          421 a          296 c          144 g          584 t
ORIGIN      
        1 gatcagtttt tatttgtcca aattttgttt gaactgaact accttcaact attggaagta
       61 aatctctttg aacgccctct tttgatggat cttgtccttg atttttcttt ccacttgcaa
      121 ttttatctat ctcatctata aaaataattc cgccattttc agctcttttt aaagcctcta
      181 tttttatagc ttcagtatct agtaaacctt cactagcaac acctcttaaa agaatttttg
      241 catcttttat gcttacttct ttttttattt tttctttatt taagcttcct aacattttgt
      301 taagactctc ttgcatagaa ctcatatcaa atggtaaatt agaatcaatt atctctacat
      361 gagctttttt aggtagttct atctctattt ttttatcatc taaatcacca tttaataact
      421 tcttttccat tagattataa gtttttataa acgactcttt tgcattttca cttgcactat
      481 caggaagtgg aggtactaat ttttcaatga tctttttatt tacttcatca tcaattttat
      541 ctttaatttt ctcttcatac tcttttgtaa caagatttat actttcataa agaaggtctc
      601 taatcataga ttcaacatct cttcctacaa acccaacttc tgtatattta ctagcttcta
      661 cttttacaaa aggaagtccc atcatttttg ccattcttct tgcaatttca gttttaccaa
      721 ctcctgtgct tccaatcatt agaatatttt ttggcataat ctcttcttgc agatttggct
      781 caactctcat tcttctataa cgatttctta aagctagagc tatggtcttc tttgcatctt
      841 tttgaccaat aatataatca tctaaataag caacaatctg cctaggtgtc atatccatac
      901 tgtttttatt cctctatttt taatattttt atattttgat ttgtataaat acaaagctca
      961 ccagcaatca tcaaagactc tcgaactaat tcttcttcat caagctgtga atgttttgct
     1021 aatgctctag cagcagagat tgcaaaattt ccaccacttc caatacttgc tatctcgcca
     1081 tcttcaggct caacaacatc tccatttccg cttaaaataa atattttttc tttatttaaa
     1141 actatcatca tagcttcaag tcgtcttaaa tacttatctt ttctccactc ctttgaaaat
     1201 gcaataacag actttaataa atcaccttta caagtttcaa gatgagcttc aaacatatca
     1261 aaaagattaa aagcatctgc tgtacttcct gcaaaacctg ctaaaatttg acctttatac
     1321 aaagttctta tcttagtagc atttcctttt aaaacagtgt ttccaaaaga tacctgtcca
     1381 tctccaccaa taacagattt atttttacct ttataggcta aaattgtagt tgcgtgaaac
     1441 ataat
//
LOCUS       DAOQAN010000374         1441 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_141321_length_1441_cov_2.074315, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000374 DAOQAN010000000
VERSION     DAOQAN010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1441
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141321_length_1441_cov_2.074315"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..877
                     /locus_tag="PKO10_06115"
     CDS             <1..877
                     /locus_tag="PKO10_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HNW66757.1"
                     /translation="DEKVKVYDIFENNKTIARIYIDLEARDDKRGGAWMNNWHSYYKD
                     SNNNINLPTAYIVGNFPQSTKDIPSLLRHSDVVTLFHEMGHALHHLLSKVEEPTVSGI
                     SGVAWDVVEFPSQFLEYFSYDRDILKLFAKHYQTGEVLDDEAISRLIKAKNFQSSMAT
                     VRQIEFALFDFKLHQKLYKTEFEIQELLNSIRDKFSVIKTPEYNKFQNSFSHIFSGGY
                     SAGYYSYKWAEVLSADAFYMFLDSKNVLNKELGLKYKNCVLSCGGSENMDKLFFDFAQ
                     REPKIDSLLKIDGII"
     gene            940..>1441
                     /locus_tag="PKO10_06120"
     CDS             940..>1441
                     /locus_tag="PKO10_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66758.1"
                     /translation="MTNAETILKLNEALTSLIKAYEELQGENGVLKQRVNELEDEVLN
                     LELIKEDLEKSVNELKSNTEEDKNTIYSMLGQIEGILNKTTSSIATVEETKYEEIKKV
                     DDNKAFNPVISQNLLDNSFDHKNETELELDKKEEENIFAYEQNSINILNEENSLYKKE
                     DDNKIDL"
BASE COUNT          584 a          150 c          250 g          457 t
ORIGIN      
        1 ggatgaaaaa gtaaaagttt atgatatttt tgaaaacaat aaaacaatag ctaggatata
       61 tatagattta gaagcaagag atgataaaag aggtggtgct tggatgaaca actggcactc
      121 atattacaaa gattcaaata acaatataaa tcttccaaca gcttatatag taggaaattt
      181 tcctcaatca acaaaagata ttccatcttt attaagacat agtgatgtag taacgctttt
      241 tcatgaaatg ggacatgcac ttcatcatct tttgagtaaa gttgaagaac caactgttag
      301 tggaatatct ggagttgctt gggatgttgt agagtttcca tctcagtttt tagagtattt
      361 ttcttacgat agagatattt taaaactttt tgcaaagcat tatcaaacag gtgaagttct
      421 tgatgatgag gcaatatcta gattaataaa agcaaaaaat ttccaatcat caatggcaac
      481 agttagacaa atagagtttg ctttgtttga ctttaaactt catcaaaaac tatacaaaac
      541 tgaatttgag attcaagagt tgttaaatag tattagggat aaattttctg tcataaaaac
      601 accagagtat aataagtttc aaaatagttt ttctcatatc ttttcaggtg gatatagtgc
      661 aggatattac tcttataagt gggctgaagt acttagtgca gatgcttttt atatgttttt
      721 ggattcaaaa aatgtattaa ataaagagtt gggattgaaa tataaaaatt gtgtacttag
      781 ttgtggtgga agcgaaaata tggataaatt gtttttcgat tttgcacaaa gagaacctaa
      841 aattgactct cttttgaaaa tagatggaat tatttagcta aattttgtaa taataccatt
      901 tcataaaatt tttatataag taataaagga ttaaagaata tgacaaatgc agaaactatt
      961 ttaaaactaa acgaagcttt aacaagctta ataaaggcat atgaggagct tcaaggagag
     1021 aatggagttc ttaagcaaag agtaaatgag ttagaagatg aagttttaaa tctagagttg
     1081 ataaaagagg atttagagaa aagtgttaat gagcttaaga gtaatacaga ggaagataaa
     1141 aatactattt atagtatgct tggtcaaatt gaaggaatat taaataaaac tacttcttct
     1201 atagctacag tagaagaaac aaaatatgaa gaaataaaaa aagtagatga taacaaagct
     1261 tttaatccag ttatttctca aaatctttta gacaactctt ttgatcataa aaatgaaaca
     1321 gagttagaac ttgataaaaa agaggaagaa aatatctttg cttatgagca aaacagcata
     1381 aatattttaa atgaagagaa tagtttatat aaaaaagaag atgacaacaa aatagattta
     1441 t
//
LOCUS       DAOQAN010000375         1440 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_141383_length_1440_cov_3.577617, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000375 DAOQAN010000000
VERSION     DAOQAN010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141383_length_1440_cov_3.577617"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1440)
                     /locus_tag="PKO10_06125"
     CDS             complement(<1..>1440)
                     /locus_tag="PKO10_06125"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNW66759.1"
                     /translation="SLGLKINAGHDLNLENLNKIKEKSKIDLNSLSLNGEPILIACCK
                     KDLYNSCLWLLENKIDLELENNLKESAIFYTIYSKDSKLLETLLEFGANLNHLNNKNR
                     TVLQESIHNANKKIVRFLIQKTNSFTNCDNNGNNVLFDAVSNGNMNIIKKIVSLEKID
                     LNHKNKIGDTILHLDNCYKNLDLAMYLLNQGADPTIPNNKSISYLFFAATKGLEAFSF
                     IKRAAELGFNLNIKNHENKNILMKAVEHFLEIQNREEKDSQSELIKALVHQNINVQAM
                     DNKNETIFFNITRSLDRDLIHYLLNNLEKLNLNRQNLEGLTVLSILILNGISNMDLIK
                     LYIEKGANLEFKNRDNVCVVETLINIILHLENEQNLDLIYKENLNQNAQYKDILEALT
                     KSYNLDFNRLNSKNKPLFFDSLLNFNFSLFKILRTKNLQINSTDINGNNIIFHLLELD
                     LEKRVENRRVLLNTIKNLIQTYNLSRDHER"
BASE COUNT          484 a          169 c          156 g          631 t
ORIGIN      
        1 tctttcgtgg tcacgtgaaa gattataggt ttgaattaaa ttttttatag tgtttaataa
       61 aacccttcta ttttcaactc ttttttccaa atctagctct agaagatgaa aaataatatt
      121 attaccattt atatcagttg aatttatttg caagtttttt gttcttaaaa ttttaaataa
      181 ggagaagttg aaatttaata agctatcaaa aaatagtggt ttgtttttag agtttaatct
      241 gttgaaatca agattatagc tttttgttaa agcctctaaa atatctttat attgagcatt
      301 ctgatttaga ttttctttat aaattaaatc aagattttgt tcattttcta aatgtaaaat
      361 aatatttata agagtttcaa ctacacaaac attatctcta tttttgaact ctaaatttgc
      421 acctttttca atatatagtt taattaaatc catattagat ataccattta atattaaaat
      481 tgataaaaca gtaagacctt ctaaattttg tctatttaaa tttaattttt caagattatt
      541 taataaatag tgaattaaat ctctatctaa acttcttgtt atattaaaaa atattgtctc
      601 atttttatta tccattgctt gaacatttat attttgatgt actaaagctt tgattaattc
      661 agattgacta tctttttctt ctctattttg aatttctaaa aagtgttcaa ctgctttcat
      721 taagatattt ttattttcat gattttttat atttaagtta aaacctagtt cagctgctct
      781 ttttatgaaa gaaaaagctt ctaaaccttt tgttgcagca aaaaacagat agctaattga
      841 tttgttattt gggattgttg gatctgcccc ttgatttaat aaatacatag ctaaatcaag
      901 atttttgtaa caattatcaa gatgtaaaat cgtatctcca attttatttt tatggtttaa
      961 atcaattttt tctaaagata ctattttttt tatgatattc atatttccat ttgatacagc
     1021 atcaaaaaga acattatttc cattattatc acaatttgta aaagagtttg ttttttgtat
     1081 taaaaatcta actatttttt tatttgcatt atgaattgat tcttgaagaa ctgttctgtt
     1141 tttgttattt aaatggttta aattagcacc aaattctaat aaagtttcaa gaagtttact
     1201 atcttttgag tatattgtat aaaatattgc actctctttt aggttgtttt caagctctaa
     1261 atcaattttg ttttcaagta accataaaca agagttgtat aaatcttttt tacaacaagc
     1321 tattaaaata ggttcaccat ttaaagataa agagtttaaa tctatctttg atttctcttt
     1381 tattttgttt aaattttcaa gatttaaatc atggcctgcg ttaattttaa gtcctaatga
//
LOCUS       DAOQAN010000376         1436 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_142004_length_1436_cov_2.658219, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000376 DAOQAN010000000
VERSION     DAOQAN010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1436
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142004_length_1436_cov_2.658219"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..830)
                     /locus_tag="PKO10_06130"
     CDS             complement(<1..830)
                     /locus_tag="PKO10_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HNW66760.1"
                     /translation="MKTNNKQIINTFYGVILVSFFAFIATIISEFDIFKNLGISPLII
                     GIVLGIFYANSLKSKFYKIFQDGITFSTKYILRLGIILYGFRLTFQNLQEVGLNGILV
                     AFCILFFTFIFGYIIGTKVLKMDKELAILCSAGSSICGAAAVMATQSVLKNEAYKSAV
                     AVSFVVIFGTIGMFLYPFLDKLGVFLFTSSQMGIYIGATLHEVAHVVGASNALGEIVS
                     TNAIIEKMIRVIFLVPFLILLSLWLIKTNFHTKKEKSKIVIPWFAIFFIMVVGFNSLV
                     "
     gene            930..>1436
                     /locus_tag="PKO10_06135"
     CDS             930..>1436
                     /locus_tag="PKO10_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNW66761.1"
                     /translation="MTLKELNFFYKLCENPQVTQVASELNISQSAISLAIKSLENSLN
                     EQLFDRIGKKLILNEKGKYFKEKTLPSYLALMDASTIFQENKLAGNIKIAASKTISNY
                     IMPNIYYDFLSKYKDVKLDILTINSSSIIDKILKNELDIGLIEVDTQNSSLIKEKLAD
                     DELIVVSSD"
BASE COUNT          607 a          199 c          144 g          486 t
ORIGIN      
        1 aaaactaaag aattaaatcc aactaccatt ataaaaaata tcgcaaacca aggaattact
       61 atttttgatt tctctttttt tgtatgaaaa tttgttttaa tcaaccataa agataaaaga
      121 atcaaaaatg gaactaaaaa aataacccta atcatttttt caataattgc atttgttgat
      181 actatttcac ctaaagcatt acttgcacca acaacatgag ctacttcatg aagtgttgct
      241 cctatataaa tacccatttg agaacttgta aaaagaaata ctcctaactt atctaaaaat
      301 ggatataaaa acattccaat tgttccaaaa attactacaa aagatacagc aactgcactt
      361 ttataagctt cattttttaa tacactttgt gttgccataa ctgcagctgc tccacaaata
      421 gaacttcccg cactacataa aatagctaac tctttatcca tttttaaaac ttttgttcca
      481 ataatataac caaaaataaa agtaaaaaag agtatacaaa aagccactaa aattccattt
      541 aaccctactt cttgaagatt ttgaaaagta agtctaaatc catataaaat aattcctaat
      601 cttaaaatat attttgtaga aaaagtaata ccatcttgaa atattttata aaattttgat
      661 ttcaaactat ttgcataaaa aatacctaaa actattccaa ttataagtgg actaattcct
      721 aagtttttaa aaatatcaaa ttctgaaata attgttgcaa taaatgcaaa aaaagataca
      781 agaattactc cataaaaagt gtttataatc tgtttattat tagttttcat aataaacctc
      841 cttttgtttt gaaagtataa cttctatcat ttaatttgta aaatatatta tttttaatat
      901 aatcattaat atttttaata aggaaaaata tgactttaaa agaattaaac tttttttata
      961 aattatgtga aaatccacaa gttacacaag ttgcaagtga actaaatatt agtcaatcag
     1021 ctatttcatt agctataaaa tcacttgaaa atagtctaaa tgaacaactt tttgatagaa
     1081 tcggcaaaaa actaatacta aatgaaaaag gaaaatattt taaagaaaaa actcttcctt
     1141 cgtatttagc acttatggat gcatcaacaa tttttcaaga gaataaacta gctggaaata
     1201 taaaaattgc tgctagtaaa actatttcaa actacataat gccaaatatt tattatgatt
     1261 ttttgtcaaa atataaagat gtaaaacttg atattttaac tataaattca agtagtataa
     1321 tagataaaat actcaaaaat gaactagata ttggtcttat tgaggtagat actcaaaata
     1381 gcagtctaat aaaagaaaaa ttagcagatg atgaattgat tgttgtatca agtgat
//
LOCUS       DAOQAN010000377         1436 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_142043_length_1436_cov_2.147719, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000377 DAOQAN010000000
VERSION     DAOQAN010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1436
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142043_length_1436_cov_2.147719"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="PKO10_06140"
     CDS             complement(<1..470)
                     /locus_tag="PKO10_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNW66762.1"
                     /translation="MSLSRRDFLKSSAAASAAAAIGMSVPTDLKAQANTAESGWRWDK
                     AACRFCGTGCGIMLATKEGKIVAVKGDPAAPVNRGLNCIKGYFNAKIMYGADRLKTPL
                     LRVNKKGEFDKKGDFAPVSWKRAFDEMEKHIKIALKEKGPEGVAVFASGQHTIM"
     gene            complement(594..>1436)
                     /locus_tag="PKO10_06145"
     CDS             complement(594..>1436)
                     /locus_tag="PKO10_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014473210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66763.1"
                     /translation="TQNISKNIFYLYSNPKSSQNELDTSIEEFIYNLESLKGGNSLGK
                     LKEAPNVQIDRQMLQIEYLWSIFYQNIVKFKELIQNNTNQKELQNIVNIIYETNPELL
                     YEVDALVSLHTINSEQKIRFLKNSQYFFAILILFLIIYSFIELKTMEKNALKFIEESK
                     KVMEQNLEEPLKPIKIEAEGELIEASNIFNRFLNKINSAIIDSNSALEQSKNASYKLE
                     EISNEFDEIINELQNKSEISKQLNKSEDIAIQTQEQLLHSSKRLNELKNELEKIILFA
                     EKKS"
BASE COUNT          426 a          231 c          177 g          602 t
ORIGIN      
        1 tccataattg tgtgttgtcc acttgcaaat acagcaactc cttcaggacc tttctctttt
       61 agtgcaattt tgatatgttt ttccatctca tcaaatgctc ttttccaaga aacaggagcg
      121 aaatcacctt ttttatcaaa ttcacctttt ttatttactc ttaaaagtgg agttttaagt
      181 ctatcagctc catacataat ttttgcatta aagtaacctt ttatacagtt aagccctcta
      241 ttaactggag ctgctggatc tcccttaaca gcaacaattt tcccctcttt tgttgcaagc
      301 ataattccac aacctgttcc acaaaatcta caggctgctt tatcccaacg ccaaccactc
      361 tcagcagtat ttgcttgtgc ttttaagtct gttggtacac tcataccaat agctgctgct
      421 gcacttgccg ccgctgaact ttttagaaaa tctcttcgtg aaagtgacat tcctactcct
      481 tttgaaataa atttataaac aataagatat tactaggaag tagatataaa aagtttgacc
      541 gatgtcaaac tttttgagta tttaagtaaa gattaaaatt ttttaattaa gttttagctc
      601 tttttttctg caaaaagtat aattttttca agttcatttt taagttcatt taatctttta
      661 cttgagtgaa gaagctgttc ttgagtttgt attgcaatat cttcactttt atttagctgt
      721 tttgatattt cacttttatt ttgaagttca ttaataattt catcaaactc atttgagatc
      781 tcttctagtt tatatgaagc attttttgat tgttctaaag cactatttga gtcaattatt
      841 gcactattta ttttatttaa aaatctattg aatatatttg aagcctctat tagttcacct
      901 tcagcttcaa tttttatagg ttttaaaggc tcttctaaat tttgttccat aacttttttt
      961 gactcttcta taaattttag agcatttttt tccatagttt ttaattcaat aaaactataa
     1021 ataattaaaa atagaattaa aattgcaaaa aagtattggc tatttttcag aaatcttatt
     1081 ttttgttcac tgtttatcgt gtgaagacta actagtgcat ctacttcata aagaagttct
     1141 ggatttgttt catatattat atttacaata ttttgcaact ctttttggtt ggtattattt
     1201 tggattaact ctttgaattt aactatattt tgatagaaaa ttgaccataa atattctatt
     1261 tgtaacattt gtctatctat ttgaacattt ggagcttctt ttaattttcc taaactattt
     1321 ccacctttta aactttcaag attatatata aactcttcaa tactagtgtc tagctcattt
     1381 tgtgaagatt ttggatttga atacaagtaa aagatatttt tagagatatt ttgtgt
//
LOCUS       DAOQAN010000378         1435 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_142131_length_1435_cov_2.806522, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000378 DAOQAN010000000
VERSION     DAOQAN010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1435
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142131_length_1435_cov_2.806522"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="PKO10_06150"
     CDS             <1..576
                     /locus_tag="PKO10_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HNW66764.1"
                     /translation="ELAEALDLTQANFATMKNRGKIPYTNILNFCAKRKISINWLLYN
                     QNPNSLIDSTDRYWIKYYPTVNVSAGGGAYDSEDYCESLELPDCFLTMLGGKENLKNI
                     DAINVVGDSMEPTLNSNNIIFVDKTKNDISRDGIYAFTTNHGLFVKRVQKRVDGLLDI
                     ISDNKDYPVQILKKDELNILGKVISSFGKIY"
     gene            complement(573..>1435)
                     /locus_tag="PKO10_06155"
     CDS             complement(573..>1435)
                     /locus_tag="PKO10_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan synthetase"
                     /protein_id="HNW66765.1"
                     /translation="LKIALQNGAYAVIFEKDFEVIDNEIAFIKVKNLELALLKIVRYK
                     LSTLKIKSYFCTDETFDMLKLYQNNHTKPIFLISKNIEKVFKFIDDIKDGDILISKNK
                     KLLESIYPDSKEFEKKLDENSIKNLIKHSLFELSFSYKDIYFSKLRLSKIYLNSFLNI
                     YDFFKGNIDISKLKLYSNFKAIFIDKDFQPIESGKSDSFIICQTNKNLIPIEITYLKN
                     EFRYAKTIFVSKYKISFLDEKEQIIINNIEDLKNILKNLKFNCVYLIGFTNQESFEFL
                     QNSQKLQALF"
BASE COUNT          544 a          148 c          168 g          575 t
ORIGIN      
        1 gagttagctg aggctttgga tttaactcaa gcaaattttg caacaatgaa aaatagagga
       61 aaaattccat atacaaatat tttaaatttt tgtgcaaaga gaaaaatttc aataaattgg
      121 cttttgtata atcaaaatcc aaactcttta atagatagca ctgatagata ctggataaaa
      181 tattatccaa ctgtaaatgt aagtgctgga ggtggagctt atgatagtga agattattgt
      241 gaaagtttgg aacttcctga ttgtttttta actatgttag gaggaaaaga aaatcttaaa
      301 aatatagatg ctatcaatgt tgttggtgat tcaatggaac ctacactaaa ctcaaataat
      361 ataatttttg ttgataaaac taaaaatgat ataagtagag atggaatcta tgcctttaca
      421 acaaatcatg gtctttttgt aaaaagagtc caaaaaagag tagatggact tttagatatt
      481 atttctgaca ataaagatta tcctgttcaa attttaaaaa aagatgaact aaatatttta
      541 ggaaaagtta ttagttcatt tggaaaaata tattaaaata gagcttgtaa tttttgagag
      601 ttttgtagaa attcaaaact ctcttggttt gtaaaaccta ttaaataaac acagttaaat
      661 ttcaaatttt ttaaaatatt ttttaaatct tctatattat ttataattat ctgttctttt
      721 tcatctaaaa aagatatttt atattttgaa acaaatatag tttttgcata tctaaattca
      781 ttttttagat atgtaatttc tataggaatt aaatttttat ttgtttggca aattataaaa
      841 ctatcacttt tcccactctc tattggttga aaatctttat caataaatat tgctttaaag
      901 tttgaatata gttttagttt tgaaatatca atatttcctt taaaaaaatc ataaatattc
      961 aaaaaactat tcaaatatat tttagataat cttaattttg aaaagtatat atccttgtat
     1021 gaaaaactaa gttcaaataa agagtgtttt attagatttt ttatactatt ttcatctaat
     1081 tttttctcaa actctttgct atctggatag atgctctcta aaagtttttt attttttgat
     1141 attaaaatat caccatcttt aatatcatca ataaatttaa aaactttttc tatattttta
     1201 gagattagaa aaataggttt tgtatgattg ttttgatata actttaacat atcaaaagtt
     1261 tcatcagtac aaaaatagga tttaattttt agagtagata atttatatct tactattttt
     1321 aataaagcta attctagatt ttttactttt ataaaagcaa tttcattatc aattacttca
     1381 aaatcttttt caaatataac tgcataagct ccattttgta aagctatttt taaat
//
LOCUS       DAOQAN010000379         1433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_142399_length_1433_cov_3.831640, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000379 DAOQAN010000000
VERSION     DAOQAN010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142399_length_1433_cov_3.831640"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(81..1421)
                     /gene="rimO"
                     /locus_tag="PKO10_06160"
     CDS             complement(81..1421)
                     /gene="rimO"
                     /locus_tag="PKO10_06160"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013136661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HNW66766.1"
                     /translation="MKFSEEKPKKKLHMVSLGCTKNLVDSEVMLGKLKDYELTDDESE
                     ADLIIVNTCGFIDSAKEESINTTLNLHNERKKDSVLVMAGCLSERYKDDLQKELTEID
                     IFTGVGDYDKIDRLVHEKRSSFSNEVFLASEENDRVITGSNYHAYVKLSEGCNQSCSF
                     CAIPSFKGKLHSRTLESLVKEVTNLVKKGYKDFSFVSQDSSSYLRDLGHNDGLEQLID
                     AVEKIDGVKTARILYLYPSTTTHNLIDKIADSKVFVNYFDMPLQHISQNMLKIMKRGK
                     GAEKLIELMNYMRSKPNSFVRTTFIAGHPGESEDDFEELCSFVEEFGFDRGNVFSYSD
                     EEGTSAETRDDKIEQELIDERAEILGDIISQTTIKSLENDIGKSFEVYIDGESEEHEY
                     LLSARKTIWAPQIDGEIYINDNEDNIPIVFGEIYEVEATQLAGDKLLAKIIRKV"
BASE COUNT          438 a          267 c          143 g          585 t
ORIGIN      
        1 actactatca actccacctg aaaaggctag aagatttttg ctatttttta tcctactaaa
       61 atcaaaaatc ataatctatt ttatactttt cttataatct ttgctagaag tttatcacca
      121 gcgagttgcg ttgcttctac ttcataaatc tcaccaaaaa ctattggtat attatcttca
      181 ttatcattta tatatatttc tccatcaatt tgtggagccc aaatagtttt tctagcactt
      241 aaaagatatt catgctcttc actttctcca tcaatataaa cttcaaaact ttttccaata
      301 tcattttcta aagatttaat agttgtttga gaaattatat cacctaaaat ttcagctctt
      361 tcatctatta actcttgctc aattttatca tctctagttt ctgctgatgt tccttcttca
      421 tcagaatatg aaaaaacatt tcctctatca aatccaaact cttctacaaa agagcaaagt
      481 tcttcaaaat catcttcact ctctcctgga tgtccagcta taaaagttgt tcttacaaaa
      541 gagtttggtt ttgatctcat atagttcatg agttcaatta atttttcagc acctttacct
      601 cttttcataa tttttaacat attttgagat atatgttgaa gtggcatatc aaagtagttt
      661 acaaaaactt ttgagtctgc aattttatct atcaaattgt gtgtagttgt actaggatat
      721 agatataaaa ttctggctgt ttttactcca tctatttttt caacagcatc aattaattgt
      781 tctaatccat cattgtgtcc taaatctctt aaataagatg atgaatcttg tgatacaaaa
      841 gagaaatctt tatacccttt ttttactagg tttgtaacct cttttacaag tgattcaagt
      901 gttcttgagt gaagttttcc tttaaatgat ggaatagcac aaaatgaaca actttgatta
      961 catccttcac ttaattttac ataagcatga tagtttgaac ctgttattac tctatcgttt
     1021 tcttcacttg ctagaaaaac ctcatttgaa aaactacttc ttttttcatg aaccaatcta
     1081 tctattttgt cataatctcc aacacctgtg aaaatatcta tttcagtaag ctctttttgt
     1141 aaatcatctt tatatctttc acttaaacat ccagccataa ctaaaacaga atcttttttt
     1201 ctttcattgt gtaaatttaa agttgtattt atactctctt cttttgcact atctataaat
     1261 ccacaagtgt ttactataat taaatcagct tcactctcat catctgttag ttcataatct
     1321 tttagttttc ctaacattac ttcactatca actaaatttt ttgtacatcc aaggcttacc
     1381 atatgtagtt ttttctttgg tttttcttca ctaaatttca tctaatatta tcc
//
LOCUS       DAOQAN010000380         1431 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_142723_length_1431_cov_3.208576, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000380 DAOQAN010000000
VERSION     DAOQAN010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1431
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142723_length_1431_cov_3.208576"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            455..826
                     /gene="dksA"
                     /locus_tag="PKO10_06165"
     CDS             455..826
                     /gene="dksA"
                     /locus_tag="PKO10_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HNW66767.1"
                     /translation="MANKSQTDELKAILLERRETILSNVRNSRESIDQLKDQDLNDDL
                     DYADFVSDSFKEGMIANHQLDELNQIEEALKKIKASTYGICDMCGVNIPIGRLKAKPF
                     AKFCTECRTVYEQEQQKRVAY"
     gene            823..>1431
                     /gene="truD"
                     /locus_tag="PKO10_06170"
     CDS             823..>1431
                     /gene="truD"
                     /locus_tag="PKO10_06170"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HNW66768.1"
                     /translation="MIKRVYPANDKVLNFKFVQNDVDFIVEEQPIKFSSYGNFLILKI
                     KKQNCDTWELIDRLSKFLGVYSSDIGYAGLKDKRATTIQYISIPKKYQKEIKNFKSKK
                     IEILDTFLHNKKLNIGDLKGNRFKINLHELELEELFHIEKLLKFVSKNGFPNYFGYQR
                     FGKDVKENLEKAKDLLFGDAIIKDRKVAKMLLSAYQSTFFNAW"
BASE COUNT          611 a          147 c          210 g          463 t
ORIGIN      
        1 ataaaaataa ctgttgataa taaaaatatt gttaaagttg ctaagataaa aatagatata
       61 tattttgttt tttgtttatg aagtaacaga aaaataagat ttataaaaac gctactcttc
      121 ataaacaaat ccaaaattag ttacttttgg gttcaaataa attacattat taaaactatt
      181 tccataatct ttaaatctat agtccttgtt tgagtatgca agatttgata atttagtaat
      241 atctaaaata tgataatttc tactattttt aaaaatagta tttgaatata agaataaaaa
      301 aaagaaaata aatgataata aaaatatagt tatcaaataa actttgctta gtgttgtaat
      361 gccctactcc tcgtactgat gaaatattct accaatttta agttaaatat ctgttaataa
      421 tagatataat tgcgatttta cacaggagag gataatggca aataaaagtc aaactgatga
      481 attaaaggcg atattacttg aaagaagaga gacaatttta agtaatgtta gaaatagcag
      541 agagagtatt gaccaactaa aagatcaaga tttaaacgat gatttagatt atgctgattt
      601 tgttagtgac tcatttaaag agggaatgat tgcaaatcat caactagatg agttaaatca
      661 aattgaagaa gctttaaaaa aaataaaagc ttcaacttat ggaatttgtg atatgtgcgg
      721 agtaaatatt ccaattggaa gattaaaagc aaaacctttt gcaaagtttt gtacagagtg
      781 cagaacagtt tatgaacaag aacaacaaaa aagagttgcg tattgataaa aagagtttat
      841 ccagcaaatg ataaagttct aaatttcaaa tttgtccaaa acgatgttga ttttatagtt
      901 gaagaacaac ctataaaatt ctcatcgtac gggaacttct taattttaaa aatcaaaaaa
      961 caaaattgtg atacttggga gttaatagat agattatcaa aattcttggg tgtttattca
     1021 agtgatattg gatatgctgg actcaaagat aaacgagcaa ctacaattca atacatatct
     1081 attccaaaaa aatatcaaaa agagataaaa aattttaaat caaaaaaaat agagatactt
     1141 gatacttttt tacacaataa aaaactaaat attggtgatt taaaaggaaa tagatttaaa
     1201 ataaatcttc atgagttaga gttagaagaa cttttccata ttgaaaaact tctaaaattt
     1261 gtatccaaaa atggttttcc aaactatttt ggttatcaaa gatttggaaa agatgtaaaa
     1321 gagaatttag aaaaagcaaa agatttacta tttggtgatg caataataaa agatagaaaa
     1381 gtagcaaaaa tgcttcttag tgcctatcaa agtacttttt ttaatgcttg g
//
LOCUS       DAOQAN010000381         1431 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_142782_length_1431_cov_2.248547, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000381 DAOQAN010000000
VERSION     DAOQAN010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1431
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142782_length_1431_cov_2.248547"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..1035)
                     /locus_tag="PKO10_06175"
     CDS             complement(52..1035)
                     /locus_tag="PKO10_06175"
                     /inference="COORDINATES: protein motif:HMM:NF024706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HNW66769.1"
                     /translation="MSEMIIYQDDSGTTNLEVELENETLWLSQKQLSELFDVTTATIN
                     EHIKNIYYEKELEKDSTIRKFLIVQQEGTRNVKRDIEHYNLDMIISVGYRVKSNIATR
                     FRQWATARIKEYIIKGFVMDDERLKQAKNSYFDELLERIRDIRSSEKLFWQKILDIYA
                     TSIDYDPSDEASQEFFKTIQNKMHFATHGHTAAEIIYQRVDSTKPFIGMTNFSGCKPT
                     KKEASIAKNYLNNEELDTLNRIVTTYLEFAELQAKKRKTMKMRDWIKKLDDFLSLSDF
                     DVLQTKGKISHTQALKKANEEYEKYKVQMINEKSKVQIDFEKVVASLGDKK"
     gene            complement(1032..>1431)
                     /locus_tag="PKO10_06180"
     CDS             complement(1032..>1431)
                     /locus_tag="PKO10_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009496266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HNW66770.1"
                     /translation="VIGLPANLFFGTSIPACILIFKKNRKDKDIIFIDASKEFEKGKN
                     QNNLTDEHIAKIFDTYKNRSEIKKYSHVATLEEIKENDYNLNIPRYVDTFEEEEIIDL
                     EATKTNIANIENELVEIKSKMSGYLRELGL"
BASE COUNT          396 a          246 c          158 g          631 t
ORIGIN      
        1 gctttatctc ttcaagtgtt tgatggagtt gtagttctaa atcttttttc attatttttt
       61 atcccctaaa gatgcaacaa ctttttcaaa gtcaatttgc actttacttt tctcatttat
      121 catttgaact ttatactttt catactcttc attggctttt tttaaggctt gtgtgtgtga
      181 tattttacct tttgtttgaa gcacatcaaa atcagataaa cttaaaaaat catcaagttt
      241 ttttatccaa tctctcattt tcatagtttt tctttttttt gcttgtaact ctgcaaactc
      301 aagatatgtg gttactattc tatttaaagt atccagttct tcattattga gataattttt
      361 agctattgat gcctcttttt ttgtaggttt acatccacta aaatttgtca ttcctataaa
      421 tggttttgta ctatctactc tttgataaat tatttcagca gctgtatgcc catgtgtagc
      481 aaaatgcatt ttattttgta tcgttttaaa aaactcttga gatgcttcat cgcttggatc
      541 ataatctata ctagtagcat atatatcaag aattttttgc caaaatagtt tctcactact
      601 tcttatatct ctaattcgtt caagaagttc atcaaaataa ctatttttag cttgttttaa
      661 tctttcatca tccattacaa aacctttgat gatgtactct ttaattcttg ctgtagccca
      721 ctgtctaaat cttgtagcaa tatttgattt tactctataa cctacagaaa taatcatatc
      781 aagattatag tgttctatat ccctttttac atttcttgtt ccctcttgtt gaactattag
      841 gaatttccta atagttgaat ctttttcaag ctctttttca tagtatatat ttttaatatg
      901 ctcatttata gtagctgttg ttacatcaaa taactcagat aattgttttt gagataacca
      961 taaagtttca ttttcaagtt ctacttcaag atttgtagtg ccactatcat cttgatatat
     1021 tatcatttca ctcatagccc aagctcccta agatacccac tcatttttga ttttatctcc
     1081 actaactcat tttcaatatt tgctatattt gtttttgtag cttcaaggtc gataatctct
     1141 tcttcttcaa aagtatctac atatcttgga atatttaaat tataatcgtt ttcttttatc
     1201 tcttctagtg tcgctacatg tgagtacttc tttatctcac ttctgttttt ataagtatca
     1261 aatatttttg ctatatgctc atctgtaagg ttattttgat tttttccttt ttcaaactcc
     1321 ttacttgcat caataaatat aatgtcttta tcttttctat tttttttgaa aatcaatata
     1381 caagctggta ttgaagttcc aaaaaataag tttgcaggta agccaataac t
//
LOCUS       DAOQAN010000382         1429 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_143133_length_1429_cov_1.908297, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000382 DAOQAN010000000
VERSION     DAOQAN010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1429
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143133_length_1429_cov_1.908297"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            182..>1164
                     /locus_tag="PKO10_06185"
     CDS             182..>1164
                     /locus_tag="PKO10_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002952114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HNW66771.1"
                     /translation="MVAERLVTNNSLKAAITSVASVPLLIDGTKELFNDGLTSHVLEA
                     AAVAISIYRKDYLAANSTNAMLELGEYIEETTVHKSDDLLKELARPNIEEAWVERKVD
                     GKVVEVLVKTAELKVGDIVVVGAGSTIAIDGHIIDGAGTINQVSMTGEAEPIAKYRGD
                     RVISGTIVEEGRFRIWAEQVGANTATQRIKHYIENSLNEKSSAQLKANRLADKLVPVT
                     LGLATAAYIFTKDFQRVASVLQADYSCALKLATPVAFKSTISKAGHSGIMIKGAKSIE
                     ALSSADTFVFDKTGTLTSGELEVISVDSYDKNWSEEKLLNLTASTEEHYFHP"
     assembly_gap    1165..1174
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1175..>1429
                     /locus_tag="PKO10_06190"
     CDS             <1175..>1429
                     /locus_tag="PKO10_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNW66772.1"
                     /translation="EAVVKAAKERGFVHVHHEEVQFIVAHGVKTEIKGKNVVIGSRHF
                     LEDDEKIDFSSYKKEIENSLKKSDTLLYVGYDGKLLGTIGL"
BASE COUNT          524 a          195 c          276 g          424 t
ORIGIN      
        1 gcacagacct tgttgtattt gtccccatta aaatatcttt caagatatag aaaagagatt
       61 aaaaactatt actttagaaa atctcttaaa atctgcatca aaaaatgaag ctagtgtttg
      121 tgtatcttgt acatcaaaca aagagccatc tataaaagga actctacttg caactactgc
      181 tttggttgcc gaaagattgg ttacaaataa tagtttaaaa gctgctatta caagcgttgc
      241 ttcagttcct cttctaattg atggtacaaa agagcttttt aatgatggtt taacttctca
      301 tgtacttgaa gcagcagctg tagctatttc tatttataga aaagattatc ttgcagcaaa
      361 ctcaacaaat gctatgttag agcttggtga atatatagaa gaaactactg ttcataaaag
      421 tgatgattta ctaaaagagc tagctcgtcc aaatattgaa gaagcttggg ttgaaagaaa
      481 agtagatgga aaagtagtag aagttttagt aaaaacagct gaactaaaag taggtgatat
      541 tgtagtagtt ggagcaggaa gtactattgc aatagatggt catataatag atggagcagg
      601 tacaattaac caagtatcaa tgactggaga ggctgaacca atagcaaaat atagaggaga
      661 tagagtaata tctggaacta ttgtcgaaga aggaagattt agaatttggg ctgagcaagt
      721 tggtgcaaat acggcaactc aaagaataaa acattacata gaaaactctt taaatgaaaa
      781 gtcatctgct cagctaaaag caaatcgact tgcagacaag ctagttcctg ttaccctagg
      841 attagcaacc gctgcttata tttttacaaa agattttcaa agagttgcat ctgtacttca
      901 agctgattac tcttgtgctt taaaacttgc aactcctgtt gcttttaaat ctacaatttc
      961 aaaagctggt catagtggaa ttatgataaa aggtgcaaaa tctattgaag cactaagtag
     1021 tgctgatacc tttgtttttg ataagacagg aactcttaca agtggtgaat tagaagttat
     1081 aagtgttgat tcctatgata aaaattggag tgaagagaag cttttaaatc ttacagcttc
     1141 aacagaagag cactattttc atccnnnnnn nnnngaagct gttgtaaaag cagcaaaaga
     1201 gagaggtttt gtacatgtgc atcacgaaga ggttcaattt atagtagccc atggtgtaaa
     1261 aacagaaata aaaggtaaaa atgtagtaat aggaagcaga cattttttag aagatgatga
     1321 aaaaatagat ttttcttcat acaaaaaaga gattgaaaac tctttaaaaa agagtgatac
     1381 tttactatat gttggatatg atggtaaact tttaggaact attggactt
//
LOCUS       DAOQAN010000383         1424 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_143814_length_1424_cov_3.357925, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000383 DAOQAN010000000
VERSION     DAOQAN010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143814_length_1424_cov_3.357925"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="PKO10_06195"
     CDS             complement(<1..514)
                     /locus_tag="PKO10_06195"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNW66773.1"
                     /translation="MKINKKVISLSLVISQSILFADTSILDEIKIEENKEFKTNSINI
                     DLEKVEQSQANSVTEVFKNNSSIEVGGGAINVQRIYLRGIESSNLNISLDGAKQGKNM
                     FQHRSNELGINPDLLKVVDVNTSPDASKAGALGGSIEMSTKDAQDFVRGNKKYGAIFK
                     TGYNTNAYMKH"
     gene            672..>1424
                     /locus_tag="PKO10_06200"
     CDS             672..>1424
                     /locus_tag="PKO10_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HNW66774.1"
                     /translation="MIDIKLLQNDFEKTSISLQKKGVSIEVLENLQTLAQNTKQKRQI
                     MEEVTAEQNILSKEFPRYKKENLDVADLQQKINNLKNRKQELEDEVRVLEEELSSIVL
                     GIPNLPDENVPFGADEDENVVLEVIGTKPTFDFKPKEHWELECDWLDFQRGVKLAKSR
                     FTALKGDGARLERALINYMLDFNRARGFNEWYVPFMANSNSLLGTGQLPKFEDDLFKI
                     EGEDLYLIPTAEVSLTNLYNDEILDKNELPLLL"
BASE COUNT          513 a          190 c          211 g          510 t
ORIGIN      
        1 catgtttcat ataagcattt gtgttatatc ctgttttaaa gattgcacca tattttttat
       61 tccctctaac aaaatcttgt gcatcttttg tactcatttc aatgcttcct cctagcgctc
      121 ctgcttttga agcatcaggt gaagtgttta catcgacaac ttttagtaaa tctggattaa
      181 tacctagttc attacttcta tgctggaaca tatttttacc ctgttttgca ccatctaaag
      241 atatatttaa atttgaactt tcaattcctc ttaaatatat tctttgcaca tttattgctc
      301 ctcctccaac ttcaatagaa gagttatttt taaatacttc tgtaacagaa tttgcttggc
      361 tttgttcaac tttttctaaa tcaatattaa tagaatttgt tttaaactct ttgttttctt
      421 caatttttat ctcatctagt atagatgtat ctgcaaaaag tatagattgc gatattacta
      481 atgataaaga gataactttt ttattaattt tcattataat ccttatataa aattttcaaa
      541 tgcaacttag ttgcaaaaag caaattctat tttaatgcaa cttagttgca tcttaattaa
      601 ataattatat ttcagtttat atggtatctt ttttagatat aattgcccaa aaattacaaa
      661 aaagagttga tatgattgat ataaaactat tgcaaaatga ttttgagaaa actagtattt
      721 ctttacaaaa aaaaggtgtc tcaattgaag ttttagaaaa cctacaaact ttagcacaaa
      781 atacaaaaca aaaaagacaa attatggaag aggtaacagc tgagcaaaat attctttcaa
      841 aagagtttcc tagatataaa aaagagaatc ttgatgttgc agatttacaa caaaaaatca
      901 ataatttaaa aaatagaaaa caagagttag aagatgaggt tagagtttta gaggaagagc
      961 tatcttcgat tgtattagga attccaaatc ttccagatga aaatgtacca tttggagcag
     1021 atgaagatga aaatgtagtt ttagaagtta ttggaactaa gccaactttt gattttaaac
     1081 caaaagagca ttgggaatta gagtgtgatt ggttagattt tcaaagaggt gtaaaacttg
     1141 caaaatcaag atttacagca cttaaaggtg atggagcaag attagagaga gctttgataa
     1201 actatatgct agattttaat agagcaagag gatttaatga gtggtatgta ccttttatgg
     1261 caaattcaaa ctctctttta ggaacaggac agcttccaaa atttgaagat gatttgttta
     1321 aaatagaagg agaagatcta tatcttattc caacagctga agttagtctt acaaatttat
     1381 ataatgatga gattttagat aaaaatgaat tacctctttt gctt
//
LOCUS       DAOQAN010000384         1412 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_145836_length_1412_cov_2.000737, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000384 DAOQAN010000000
VERSION     DAOQAN010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1412
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145836_length_1412_cov_2.000737"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="PKO10_06205"
     CDS             <1..240
                     /locus_tag="PKO10_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013135757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNW66775.1"
                     /translation="KNAKKLVEILPLIPKEKLLIETDAPYLTPHPYRGVRNEPYYTIY
                     VAQKMAEILNISDEEIQSITTNNAKNLFKEFSNIS"
     gene            276..>1412
                     /locus_tag="PKO10_06210"
     CDS             276..>1412
                     /locus_tag="PKO10_06210"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNW66776.1"
                     /translation="MKKILVTITILISSIFANNFEQNMVILEELNLPKEFLNEQSFKS
                     KYKELSTTKKIQYYDNLIKKSSLNAKIVKEELELKNLPKVLFFLPLIESSFKNLVNKN
                     GPSGLWQIMPLTASNLKLKRNEFVDERLDLIKSTDAATNYLRRYYKKFGKWYLSVLAY
                     NAGEGRIIHGVARATLDKYLDEHPTMYHDKVIKIYNLYIADYTKNKKGIDNLYTIYKD
                     LGLKSGHFDYLYLLKHNSKRDYLPKTSVNYINMLVSFAILENSDRFKNLDKKTKYDLE
                     KVNLNKGLKLKTIADELSMSYEELKTINKHLLKEMVPADKNSYNFYIPHTKVELFNNK
                     ISNMKELAVTENKATLEKVKNDANNKSSFDANKNSKDLNTNKIIH"
BASE COUNT          613 a          153 c          193 g          453 t
ORIGIN      
        1 aaaaatgcaa aaaagttagt agaaattttg cctttaattc ctaaagagaa acttttaata
       61 gaaacagatg caccatattt aacaccgcat ccttatagag gagtaagaaa tgagccatat
      121 tatacaatat atgtagctca aaaaatggcg gagattttaa atattagtga tgaggagatt
      181 caaagtatta ctacaaataa tgccaaaaat ctttttaaag agttttctaa tatttcttag
      241 gtataatcaa agaattctaa aataaagagg aaaccttgaa aaaaatttta gtaacaataa
      301 ccattttaat ctcttctatt tttgcaaata attttgaaca aaatatggtt atattagaag
      361 agttaaatct ccccaaagag tttttaaatg aacaatcttt taaatcaaaa tataaagaac
      421 tatcaactac aaagaaaatt cagtattatg ataacttaat aaaaaaatca tcattaaatg
      481 caaaaattgt aaaagaggag ctggagctaa aaaatcttcc aaaggtactt tttttcttgc
      541 ctttaataga gtcaagtttt aaaaatttag ttaataaaaa tggtccttct ggactttggc
      601 aaattatgcc tttaactgct agtaatctta aattaaagag aaatgagttt gttgatgaga
      661 gattagattt aataaaatca acagatgcgg ccacaaacta tttaagaaga tattataaaa
      721 aatttggaaa atggtatttg tctgttttag cttataatgc aggtgaggga agaattattc
      781 atggagtagc aagagctact ttagataaat atttagatga acacccaact atgtatcatg
      841 ataaggtgat aaaaatttat aatttatata ttgcggatta tacaaaaaat aaaaaaggta
      901 tagacaattt atacactatt tacaaagatt tgggtttaaa atctggacat tttgattatc
      961 tttatctttt aaaacataat tcaaaaagag attatttacc aaaaactagt gtaaattata
     1021 tcaatatgtt agtatctttt gcaattcttg aaaatagtga tagatttaaa aacttagata
     1081 aaaaaacaaa gtatgattta gaaaaagtta acttaaataa aggattaaag ttaaaaacta
     1141 ttgctgatga gctttctatg agttatgaag agttaaaaac tataaataaa catcttttaa
     1201 aagagatggt accagcagac aaaaatagtt ataacttcta tattcctcat acaaaagttg
     1261 agttatttaa caacaaaatt tcaaatatga aagagttagc tgtaacagaa aataaagcaa
     1321 ctttagaaaa agtaaaaaat gatgcaaata acaaatcttc ttttgatgca aataaaaata
     1381 gtaaagattt aaatacaaat aaaattattc ac
//
LOCUS       DAOQAN010000385         1411 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_145928_length_1411_cov_2.820059, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000385 DAOQAN010000000
VERSION     DAOQAN010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1411
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145928_length_1411_cov_2.820059"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            362..1273
                     /locus_tag="PKO10_06215"
     CDS             362..1273
                     /locus_tag="PKO10_06215"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011371854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNW66777.1"
                     /translation="MEFKKPTILVVDDMTTTLLLLNDLLKDTYEVKIAKSGTKALEIL
                     KSPNDIDLILLDIEMPDINGYDVCKRIKNNETIKNIPIIFITGRTSQEDEEYGLNLGA
                     IDYITKPFNKAIVKLRIKNYLDLKIKNDMLEKLSMYDGLTNIRNRRFFDETFEKTFSE
                     IKRDKKSLAVLMIDIDFFKPYNDNYGHGQGDETLRKVAKALEKTIKRASDFVARYGGE
                     EFVILLKDINKDGVEAVANNLLNAVRELKITHEFSKIENYVTISIGASFYNSNSDVTK
                     LELLLKADETLYNVKNSGRNNFAILEV"
BASE COUNT          563 a          146 c          199 g          503 t
ORIGIN      
        1 ttaaatagtt taacttatca atagcatcgc ttgagtttga tacaagctct cttataaaaa
       61 tctctttatt tgagtataaa gagtgtgtca ttaggtgaag tagttgccct acttctgttt
      121 gaaattgatg ttttgccata aaaaaatctc ctttttttat ttttattata aatttttaat
      181 ttttcaaaaa ggaattttat caaaccttta ttaaacttgg ctaaagtttt ttattttttt
      241 agcactcaac ataatcaact gctaaaaaat gcacaattat aaaatttata ctatatttat
      301 aattataaat atataatcac agcattgttt acaaatttaa aaaaagttct tgggagagat
      361 tatggaattt aaaaaaccaa ctattttagt tgttgatgat atgacaacaa cactactttt
      421 acttaacgat ttactaaaag atacttatga agtaaaaatt gcaaaaagcg gcacaaaagc
      481 tcttgaaatt ttaaaatcac caaatgatat agatttgatt cttttagata ttgagatgcc
      541 tgatattaat ggttatgatg tttgtaaaag aataaaaaac aatgaaacta taaaaaatat
      601 tccaataatt tttataacag gaagaacaag ccaagaagat gaagagtatg gattaaactt
      661 aggagctatt gactatatta caaaaccgtt taacaaagca attgtaaaac taagaataaa
      721 aaactatctt gatttaaaaa ttaaaaatga tatgcttgaa aaattatcta tgtatgatgg
      781 tttaacaaat attagaaata gaagattttt tgatgaaact tttgaaaaaa cattcagtga
      841 aataaaaaga gataaaaaat ctttagctgt tttgatgata gatatagatt ttttcaaacc
      901 ttataacgat aactatggtc atggacaagg agatgagact ttaagaaaag ttgcaaaagc
      961 tcttgaaaaa actataaaaa gagcatctga ttttgttgct cgttatggtg gtgaagagtt
     1021 tgtaatttta ttaaaagata taaacaaaga tggtgttgaa gcagttgcaa ataatctttt
     1081 aaatgctgtt cgagagttaa aaatcactca tgaattttca aaaatagaaa actatgtaac
     1141 aattagtatt ggagcatctt tttacaactc aaactcagat gttacaaaac tagagttact
     1201 gctaaaagca gatgaaactt tatataatgt taaaaatagt ggtagaaata attttgcaat
     1261 cttagaggtt tagttatgaa aaatgactct ttaaagtata agtttttagg tttaataatc
     1321 tttttattta tttgttcatt tagctctttg gtttatattt tttatgattt tcaaaaacaa
     1381 gaattaaaga ttttaaaaga tgagcaaatt t
//
LOCUS       DAOQAN010000386         1409 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_146241_length_1409_cov_3.002954, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000386 DAOQAN010000000
VERSION     DAOQAN010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146241_length_1409_cov_3.002954"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1334)
                     /locus_tag="PKO10_06220"
     CDS             complement(<1..1334)
                     /locus_tag="PKO10_06220"
                     /inference="COORDINATES: protein motif:HMM:NF019874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNW66778.1"
                     /translation="MELQNVKKYINKRKLLILSIATIVLISIIIAIDSIYSLKSFSSE
                     TIEKYKEEAYAKKEEELKNYVSLAMKTVEAYHQRTSVEKVKIEVEEELKKQTMFLFSI
                     LEAEYEKNKNILSKDALQERLKDIVNATRYSKTGYFWINDLDAVVLIHPINPKLNNKD
                     MHEYKDPNGKQIFKEFADIAKKDKEGFIDYVWPKPGFDQPQLKVSFVKLFAPYNWVIG
                     TGEYVENATAKIQAEALKTISEMRYANNDYFWINDSHPKMINHPTNSKLNGTDLSNYA
                     DPYGTKLFVEMARVTNEKKEGGLVKYYWDKPNKKNDPKEKFSYVQKFEPWDWIIGTGA
                     YVDDIEAEVALMQENTNAKIETIIASIALFSFISVIIAILIYNYFVNKAIIRPLDELD
                     IAIKDIASGNEQADRINKKSDDEIGKIVDSFNQYISQLKKGYIEDGQVIENV"
BASE COUNT          436 a          234 c          151 g          588 t
ORIGIN      
        1 tctacatttt ctataacttg accatcttca atataacctt ttttaagttg agatatatat
       61 tgattaaaac tatcaacaat ttttcctatc tcatcatctg attttttatt tattctatct
      121 gcttgctcat ttccacttgc tatatctttt atagcaatat caagctcatc aagtggtctt
      181 atgatagctt tatttacaaa atagttataa attaaaattg ctataattac tgaaataaaa
      241 gagaatagag caatacttgc tattattgtc tctatttttg catttgtatt ctcttgcatc
      301 aaggcaactt ctgcttcaat atcatcaaca taagcacctg ttcctattat ccaatcccaa
      361 ggctcaaatt tttgtacata agagaacttc tctttaggat catttttctt atttggttta
      421 tcccaataat attttacaag tccaccctct tttttctcat ttgtaactct tgccatctct
      481 acaaagagtt ttgtaccata aggatcagca taattactta aatctgttcc atttaattta
      541 ctatttgtag ggtggttaat cattttaggg tgagaatcat ttatccaaaa atagtcatta
      601 tttgcatatc tcatctctga aatagttttt aaagcttctg cttgaatttt agctgtagca
      661 ttttcaacat attcaccagt tccaataacc cagttatatg gagcaaataa ctttacaaaa
      721 gatactttaa gttgtggttg gtcaaaacct ggttttggcc aaacataatc aataaaacct
      781 tctttatctt tttttgcaat atctgcaaac tctttgaata tttgtttccc atttggatct
      841 ttgtattcat gcatatcttt attatttaac ttaggattaa ttgggtgaat taaaacaacc
      901 gcatctaaat catttatcca aaaatatcct gttttactat atctagtagc atttacaata
      961 tcttttaatc tctcttgtaa agcatcttta gataggatat ttttattttt ttcatactct
     1021 gcttctaaaa tagaaaaaag aaacatagtc tgttttttaa gctcttcttc aacctcaatt
     1081 ttaacttttt ctacagaagt tctttgatga taagcttcaa ctgttttcat agctaacgaa
     1141 acatagtttt taagctcttc ctcttttttt gcataagctt cttctttgta cttctctatt
     1201 gtttcacttg aaaaagattt aagtgaataa atcgagtcaa tagctataat tattgatatt
     1261 aagacaattg tcgcaattga taatattaat aattttcttt tattgatata ttttttgaca
     1321 ttttgtagct ccaatgcaat taaatgccat tattatatca ttatttaaaa aaaatctata
     1381 ttttttagct ctttaataaa ttttttaca
//
LOCUS       DAOQAN010000387         1408 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_146426_length_1408_cov_2.512195, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000387 DAOQAN010000000
VERSION     DAOQAN010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1408
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146426_length_1408_cov_2.512195"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(111..812)
                     /locus_tag="PKO10_06225"
     CDS             complement(111..812)
                     /locus_tag="PKO10_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNW66779.1"
                     /translation="MLELKNFNNFILKDISFILEKNENLLILGENGAGKSTLAKVLSN
                     LLPSSNLFFESKNIKDLSSYDRAKYINYIPSRFEIFDEYLNVLEYLKLSIIEEKNIED
                     IEKIISLLKLEDLKLNSCVDLSSGEQQLLLLASALLHNAKITIFDELTANLDLNRVKD
                     VFDILNSSLLSQKIVITHNLDFAYALKDFKVLFLQKGSLKFFGSHSEFFIQENLNRFY
                     GENLKLLDSHLVLDL"
     gene            complement(790..>1408)
                     /locus_tag="PKO10_06230"
     CDS             complement(790..>1408)
                     /locus_tag="PKO10_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNW66780.1"
                     /translation="KIELELCEDKQDYDKKLNQSLKDLNFKTLVYKTNSLEDIKFTIK
                     DLGEVFNKKEEAKYLNNQIENSLESLKNIIENQKILIVISPQSTLSNQIFVVGNFIYF
                     EDIIKASNNINAYQSSLKSQPSINSEKLITLNPDIIILLAPYLKDDKEKDDMLNLWKK
                     LPVNASKKENIYIIDDEYSGISSHRVKYLIDDFKGILEDVRAKKF"
BASE COUNT          519 a          174 c          120 g          595 t
ORIGIN      
        1 agtagataaa ctattattaa aattgaaaat atctttaata tttattgtaa tttctcctaa
       61 aaaaggagat aaaaatataa tcaaaatagc aaaaatatat aatatttttt tcataaatca
      121 agcaccaaat gtgagtctaa aagttttaaa ttttcaccat aaaatctatt taaattctct
      181 tgtataaaaa attcactatg agagccaaaa aatttcaaag aacctttttg taaaaacagt
      241 actttgaaat cttttaaagc ataagcaaaa tcaagattat gtgtaattac tattttttga
      301 gaaagtaagc ttgaatttaa aatatcaaaa acatctttta ctctatttaa atctaaattt
      361 gcagttaatt catcaaaaat tgtaatcttt gcattgtgaa gtaaagcact agcaagaagc
      421 aaaagttgtt gctctcctga acttaaatct acacaagagt ttaattttag atcttcaagt
      481 tttagtaaag atataatttt ttctatatct tctatatttt tttcttctat aattgatagt
      541 tttaaatact ctaaaacatt caaatattca tcaaatattt caaaacgact aggtatataa
      601 tttatatatt ttgctctatc atatgagctt aaatctttta tatttttact ttcaaaaaaa
      661 agattagaag atggtaaaag atttgataaa acttttgcta aagttgattt acctgctcca
      721 ttttcaccta aaataagtaa attttcattt ttttctaaaa taaaagatat atcttttaaa
      781 ataaaattat taaaattttt tagctctaac atcttctaaa atacctttaa aatcatctat
      841 taaatatttt actctgtgac ttgagattcc tgagtattca tcatctatta tataaatatt
      901 ctctttttta cttgcattta ctggtaactt tttccaaaga tttaacatat catctttttc
      961 tttatcatct tttaaatatg gcgctaataa tataataata tctggattta aagtaatcaa
     1021 tttttcacta tttattgaag gttgtgattt taaacttgat tgataggcat ttatattgtt
     1081 actagctttt ataatatctt caaaatatat aaaatttcct actacaaaaa tctgatttga
     1141 tagagtactt tgtggactta ttactataag tattttttga ttttctataa tattttttaa
     1201 agattccaaa ctattttcta tctgattatt taaatatttt gcttcctctt ttttattgaa
     1261 aacttcacct aaatctttta ttgtaaactt aatatcttct aaactatttg ttttataaac
     1321 caaagtttta aaatttaaat cttttaaact ttggtttaat tttttatcat aatcttgttt
     1381 atcttcacat aattctaatt caatttta
//
LOCUS       DAOQAN010000388         1408 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_146459_length_1408_cov_2.014043, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000388 DAOQAN010000000
VERSION     DAOQAN010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1408
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146459_length_1408_cov_2.014043"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="PKO10_06235"
     CDS             <1..437
                     /locus_tag="PKO10_06235"
                     /inference="COORDINATES: protein motif:HMM:NF024509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AsmA-like C-terminal domain-containing protein"
                     /protein_id="HNW66781.1"
                     /translation="AIPAVFGLNKLGIYKIKEGVVEFEYDNDKNLIDIKNLHTVGNGM
                     DFEGKGVLDLNKKEVDATLNLVFLKTYSSVFEYIPIVNYILLGDEKRVETQIQLTGSL
                     SDPVLTTNLFKDGFSAPLNIFKRVFTTPSNMIDNFNKFNQKD"
     gene            525..>1408
                     /locus_tag="PKO10_06240"
     CDS             525..>1408
                     /locus_tag="PKO10_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HNW66782.1"
                     /translation="MENLIKKLDLNEYITNFSKLFAREKSIILEGDINIHYKIIGELS
                     KYNINQPLSVQNLDSQLMHLQKQGVLRVYEIYEFIKIINYFLYLKRFNFEGKLFEWMD
                     KIIIPNDILKICEYFDDKANLKEGVDEDFDNIKQAIYKNKEEIKQSLYKTINSSKIKP
                     YLVDMQVHFINEQECLLVRGGFNHILSGSVIDRSNSGFFYVIPHSVSDLKEKQSDLKN
                     KQEEILFKLCKEISHIFEKNLLFLKFINKEFDKFDHYQARIFFSKIGDKNFILPSKKD
                     ENRLVDFCHPALSSPKPIS"
BASE COUNT          570 a          136 c          205 g          497 t
ORIGIN      
        1 tggctattcc tgctgttttt ggcttaaata aattaggaat ttacaaaata aaagagggtg
       61 ttgttgaatt tgaatatgat aacgataaaa atttaattga tataaaaaat ttacacacag
      121 ttggaaatgg tatggatttt gaaggaaaag gagttttaga tttaaataaa aaagaggtag
      181 atgcaactct taatttggta tttctaaaaa catattcatc tgtatttgaa tatattccaa
      241 ttgtaaatta tattttattg ggagatgaaa agagagttga aacccaaatt caattaacag
      301 gaagcttaag tgatccagtt cttactacaa atttatttaa agatggattt tctgcccctt
      361 taaatatatt taaaagagta tttacaacac cttcaaatat gattgataat tttaataaat
      421 ttaatcaaaa agattaaagg tattactgtt tttactatag ttttaatata aaaatataaa
      481 atttttcata agaaccttat tgctattttg atacaattca aactatggaa aatttaataa
      541 aaaaactaga cttaaacgaa tatatcacaa acttttcaaa actttttgca agagaaaaat
      601 ctataatatt agaaggtgat ataaatattc actataaaat aataggtgaa ctttcaaaat
      661 ataatataaa ccaaccttta agtgtacaaa atcttgattc acagttaatg catcttcaga
      721 aacaaggtgt tttgagggtt tatgaaattt atgagtttat aaaaataata aactattttt
      781 tatatctaaa aagatttaat tttgaaggaa aactttttga gtggatggat aaaatcatta
      841 ttccaaatga tattttaaaa atttgtgaat attttgatga taaagcaaat ctaaaagagg
      901 gtgtggatga agattttgac aatataaaac aagctattta taaaaataaa gaagagataa
      961 aacagagcct ttataaaact ataaactcat caaaaataaa gccttattta gtagatatgc
     1021 aggtacattt tataaatgag caggagtgtt tattagttcg tggaggattt aatcatatac
     1081 ttagtggaag tgttatagat aggtctaatt caggattctt ttatgtaatt ccacatagtg
     1141 ttagtgattt aaaagagaaa cagagtgatt taaaaaacaa acaagaggag attttattta
     1201 aactttgtaa agaaatttct catatttttg aaaaaaatct acttttttta aagtttataa
     1261 ataaagagtt tgataagttt gatcactatc aagctaggat atttttctca aaaatagggg
     1321 ataaaaattt tattttacct tctaaaaaag atgagaacag acttgttgat ttttgtcatc
     1381 cagctttaag tagtccaaag cctatttc
//
LOCUS       DAOQAN010000389         1406 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_146714_length_1406_cov_3.075500, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000389 DAOQAN010000000
VERSION     DAOQAN010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1406
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146714_length_1406_cov_3.075500"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="PKO10_06245"
     CDS             <1..507
                     /locus_tag="PKO10_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014469056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNW66783.1"
                     /translation="ITREEFEAVKKAMGIKTPQGTAKTTVESKEKLVDATTSNISSVE
                     LKTADDKIKFMADAKKDYDAKIYNSAIPKFEKLIEINYKPAENNYYLGDMLFKRKKYD
                     QAIVHFKKSAMLNDKASYMPTLLLNSAISFENTKDKENAKNFYSTLIELYPNSQEAKT
                     AKTNLNKL"
     gene            519..1112
                     /locus_tag="PKO10_06250"
     CDS             519..1112
                     /locus_tag="PKO10_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002934149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNW66784.1"
                     /translation="MSNKVIGIEYTLKDAKTGEQLDTNVGQAPLEFVSGKGQIIKGLE
                     DKLVNMSANEEADVLVEPKDGYGEYNDEAVQTLPKEQFAGIELSEGMSLYGQGEHGET
                     IQVVVKSFDDTSVTIDYNHPMAGRTLMFSVAILSLRDATEEEIQSGVVGGFAAMGGGC
                     CGGGGCGTGHDHDDHDHGHSHGGGCGTGGGHGGCGCH"
     gene            1198..>1406
                     /locus_tag="PKO10_06255"
     CDS             1198..>1406
                     /locus_tag="PKO10_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HNW66785.1"
                     /translation="MKKVAFIFPGQGSQTIGMGRDFFENSDTAKEMIKKASERLNIDF
                     EKLLFEENENLGKTEFTQPAILLVSS"
BASE COUNT          550 a          160 c          283 g          413 t
ORIGIN      
        1 attactagag aagagtttga agcagttaaa aaagctatgg gaattaaaac tccacaaggt
       61 actgctaaaa caacagttga atcaaaagaa aaattagttg atgcaactac ttcaaatatt
      121 agttctgttg agcttaagac tgcagatgat aaaataaaat ttatggcaga tgccaaaaaa
      181 gattatgatg caaaaattta caatagtgct attccaaagt ttgaaaaact aattgaaata
      241 aactataagc ctgctgaaaa taactactat ttaggtgata tgttatttaa aagaaaaaaa
      301 tatgatcaag ctatcgttca ctttaaaaag tctgcaatgc ttaatgataa agcttcttat
      361 atgccaacat tattgctaaa tagtgctatt tcatttgaaa atactaaaga taaagaaaat
      421 gctaaaaact tttatagtac tttaatagaa ctttatccaa atagtcaaga agctaaaacc
      481 gctaaaacaa atttaaataa attataaaag gaattaaaat gtcaaataaa gtaattggaa
      541 tagaatatac actaaaagat gcaaaaactg gtgaacaact agatacaaat gtaggacaag
      601 cgccgttaga gtttgtttca ggaaaaggtc aaataataaa aggtttagaa gataaacttg
      661 taaatatgtc tgcaaatgaa gaggctgatg ttttagttga gcctaaagat ggttatggtg
      721 agtataatga tgaagcagtt caaactttac caaaagagca atttgctgga attgagttaa
      781 gtgaaggaat gagtctttat ggtcaaggtg agcatggaga gactattcaa gtagttgtaa
      841 aatcttttga tgatacaagt gtaacaattg attataatca tccaatggct ggaagaactt
      901 taatgttttc agttgcaata ttaagcttaa gagatgcaac agaagaagag attcaatcag
      961 gtgttgttgg tggatttgct gctatgggtg gtggatgctg tggaggaggt ggttgtggaa
     1021 ctggacatga ccatgatgat cacgatcacg gacatagcca tggtggtggt tgtggaactg
     1081 gtggtggaca cggtggatgt ggttgccact aatttatttt tataaataaa taaactataa
     1141 aattgcccaa gtgtagtgat acatttgggc aatttaattt taaaacaaga ggttaaaatg
     1201 aaaaaagttg cttttatatt ccctggacaa ggaagtcaaa cgattggtat gggaagagat
     1261 ttttttgaaa atagtgatac tgcaaaagag atgataaaaa aagcaagtga aagattaaat
     1321 atagattttg aaaaacttct atttgaagag aacgaaaatc taggaaaaac tgagtttact
     1381 caacctgcta ttttactagt tagttc
//
LOCUS       DAOQAN010000390         1406 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_146757_length_1406_cov_2.274611, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000390 DAOQAN010000000
VERSION     DAOQAN010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1406
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146757_length_1406_cov_2.274611"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="PKO10_06260"
     CDS             <1..604
                     /locus_tag="PKO10_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66786.1"
                     /translation="ETVTKSNKSDDDIVKEWSSSKISEGVFHFPAENDTKVVSQLSQV
                     ANDSEEKVSQIFDVLSLTLDNNSDLRRRVKEYEEFILSQNKLLSSLSNKFPKIELFKQ
                     QLEKVKSFENSLKDLKTSIDEEDMHIFQGMELMQFNDINRQKIERVMSVIRKLSIYLN
                     NLFEDDNPRDLPMAKHIHGDKDTQDLVGDDLDKLIAEFNK"
     gene            614..1333
                     /locus_tag="PKO10_06265"
     CDS             614..1333
                     /locus_tag="PKO10_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body protein"
                     /protein_id="HNW66787.1"
                     /translation="MNQGVYPLTASMVNQINRLDQISNNLANADTFGFKQEGTSETTF
                     NWYLKRMQEEQQNPFVESITINNIPKIDTRYTDPLMGPMKMTGNPLDFALNAPDTFFK
                     IQNENGDIVYTRDGAFKNLDGFLVDGNGNNVLNADNEAIVVEDGFELQIGVAKTSFKN
                     LEKVGDNTYKALVIDDVENFENNDNKILNGTVEQSNVNRVSTMVELIDAHRRFDQSQR
                     AIKTIDELNAGLIEKIGGNTR"
BASE COUNT          581 a          150 c          253 g          422 t
ORIGIN      
        1 agaaaccgtt acaaaatcta ataaatcaga tgatgatatt gtaaaagagt ggtcatcttc
       61 aaaaataagt gaaggagttt ttcatttccc agctgaaaat gatacaaaag tagtaagcca
      121 acttagtcaa gtagcaaatg attcagaaga gaaagtttca caaatttttg atgtattaag
      181 tttaacttta gacaataata gtgatttaag acgaagagta aaagagtatg aagaattcat
      241 tttatctcaa aataaacttt taagttcttt aagtaataaa tttccaaaaa tagagctatt
      301 taaacaacag ctagagaaag taaaaagttt tgaaaactca ttaaaagatt taaaaacttc
      361 gatagatgaa gaggatatgc atattttcca aggaatggag cttatgcaat ttaatgatat
      421 aaataggcaa aaaattgaaa gagttatgtc agttattaga aaactatcta tttatttaaa
      481 taatcttttt gaagatgata atccaagaga tcttccaatg gctaaacata ttcacggaga
      541 taaagatacg caagatttag taggtgatga tttagataag cttatagctg aatttaacaa
      601 gtaaggaaat ataatgaacc aaggtgttta tccactaaca gcttcaatgg taaatcaaat
      661 aaatagactt gatcaaataa gtaataacct tgctaatgca gatacttttg gttttaagca
      721 agagggaact tctgagacta catttaactg gtatttaaaa agaatgcaag aggagcaaca
      781 aaatccattt gttgagtcaa taacaataaa taatattcca aaaattgata caagatatac
      841 agatcctcta atgggaccta tgaaaatgac tggaaatcct cttgattttg ctttaaatgc
      901 accagatact ttttttaaga ttcaaaatga aaatggtgat atagtatata caagagatgg
      961 agcatttaaa aatttagatg gatttttagt agatggaaat gggaacaatg ttttaaatgc
     1021 agataatgaa gctatagttg tggaagatgg ctttgagtta caaattggag ttgctaaaac
     1081 atcttttaaa aatcttgaaa aagtaggaga taatacttac aaggctttgg ttattgatga
     1141 tgttgaaaat tttgaaaata atgataataa aattttaaat ggaacagttg aacaatcaaa
     1201 tgtaaataga gtaagtacta tggttgagct aatagatgcg catagaagat ttgatcaatc
     1261 tcaaagggcg attaagacaa tagatgagtt aaacgctggt ttaatagaga aaattggagg
     1321 aaatactaga taatgcatgc aagtgaagta tcagggaaac tttttgaaca actaaatttt
     1381 agaggcgaaa gacaaaaagt aatatc
//
LOCUS       DAOQAN010000391         1403 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_147221_length_1403_cov_3.136499, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000391 DAOQAN010000000
VERSION     DAOQAN010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1403
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147221_length_1403_cov_3.136499"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(216..1232)
                     /locus_tag="PKO10_06270"
     CDS             complement(216..1232)
                     /locus_tag="PKO10_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008337390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNW66788.1"
                     /translation="MMYKKDEKNKNQEENFWVSYADLMAGLLFVFILVIASIVIKYLY
                     TQHSLADSEDAKSKLFYELAKTKNMYEKSLEDLKDANQKIVLSNKEVEKLKALLLEYD
                     INIKDEKSKNDSLSLALNDSSNQIALKDEELKLLADKLLVQTQIHQKMVEEFDIAKLK
                     IKTLTGIKLNVIAKLKEKLGNSIHVDEKSGAIKFSSNILFDQNSYILKEEAKKELSSV
                     LKNYLSLLLDDKEISKYIENITIEGHTNSDGTYLANLLLSQQRALAVMQFLYESNIVD
                     KKLLSTFVNSSGRSSADLILDKKGVEDKDASRRIEIKFNIKNEEAIKEIQNYLDKQNE
                     NRVQ"
BASE COUNT          438 a          218 c          143 g          604 t
ORIGIN      
        1 tttaaatatg gttattttgc gagggaagat gttgttctat ataaaacatt agagaaaaaa
       61 atattgaaat atctttttaa aagagttaaa tttttttctt cccaaataaa aacttaaata
      121 tcttttgaaa gatatttagg tttttctcta caatcttttg ctttatctct tttgaaagtt
      181 cttccaaatg agtaggcgta tatagtggtt ttttatcact gaactctatt ttcattttgt
      241 ttatccaaat agttttgtat ctcttttata gcttcttcgt ttttaatatt aaatttgatt
      301 tcaattcttc ttgaagcatc tttatcttca acaccttttt tatctaaaat caaatcagca
      361 gaacttcttc cgcttgagtt tacaaaagta cttaaaagct ttttatctac aatatttgat
      421 tcatataaaa actgcataac tgcaagtgct ctttgttgag ataataaaag atttgccaaa
      481 tatgttccat cactatttgt atgaccttca atagttatat tttctatata ttttgatatc
      541 tctttgtcat ctaataaaag tgaaagatag tttttcaaaa cactacttaa ctctttttta
      601 gcttcctctt ttaaaatata agagttttga tcaaataata tatttgatga aaattttata
      661 gctccacttt tctcatctac atgtatagag ttacctagct tctcttttaa tttagctata
      721 acatttagct ttattcctgt taaagttttt atctttaact ttgcaatatc aaactcttct
      781 accatttttt ggtgaatttg agtttgaacc aaaagtttat ctgctaaaag ttttaactcc
      841 tcatctttta aagcaatttg atttgaacta tcatttaaag ctaaagataa actatcattt
      901 ttactctttt catcttttat attaatatca tactcaagca aaagagcttt tagtttttca
      961 acctctttat tgcttaaaac tattttttgg tttgcatctt ttaaatcttc taaactcttt
     1021 tcatacatat tttttgtttt tgctaactca taaaatagct ttgattttgc atcttcacta
     1081 tctgcaaggc tgtgttgagt atataaatat tttataacta ttgatgcaat taccaaaata
     1141 aatacaaaaa gcaatccagc catcaaatca gcataagaaa cccaaaagtt ctcctcttga
     1201 tttttgtttt tttcatcttt tttatacatc atttactact ttgataaatc tttaaaaata
     1261 gtcaaatctt ttactatatt tgctgtttgt aaatctattt gtcgtaaaga ctcttttaat
     1321 tcctcttcaa atttccactc tattttttcc aaccgacaat tccgatagtt tcagaataag
     1381 ctttgagttt tctaagattg gaa
//
LOCUS       DAOQAN010000392         1402 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_147397_length_1402_cov_2.966592, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000392 DAOQAN010000000
VERSION     DAOQAN010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147397_length_1402_cov_2.966592"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1249)
                     /locus_tag="PKO10_06275"
     CDS             complement(<1..1249)
                     /locus_tag="PKO10_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HNW66789.1"
                     /translation="MNFYSKTLFLDQFTPVSIYEKTKALYPKELSFLFESSISSSNDG
                     NFSYIIIGARERIWYKNNECFFKNEIGTIEKVDNNPLLFLKKYYKNFEKNIYKEKSKE
                     LGIGLIDGFIGNVGYDMGKEFEPRLKASMNSLQDELNIPDLDLIRPKIILAFSHKTSK
                     LVILTSMNELKNEIENIEKELLKPYIYTPLKKAKLIDNGKFNFTKEEFFSIVEKSKEM
                     IRAGDIFQILISNRFTQKAVVDSLSFYRALRSKNPSPYLFLLEFEDFSIAGSSPEVMI
                     RLVDGHLLLRPIAGTRKRGSTLEKDLEMENELINDKKERAEHLMLVDLGRNDVGRVAR
                     AGSVKVTELMRIERYSHVMHIVSDVEAVLDEKYDMFDLFMATFTAGTMTGAPKIRAME
                     LIANFEKIKRNFYSGSIAYFGFDG"
BASE COUNT          444 a          244 c          146 g          568 t
ORIGIN      
        1 taccatcaaa accgaagtac gctatacttc cagagtaaaa atttcttttg attttttcaa
       61 aatttgctat taattccata gctcttattt ttggagctcc agtcattgtt ccagctgtaa
      121 atgttgccat aaataggtca aacatatcat atttttcatc taaaactgct tcaacatctg
      181 aaactatatg cataacgtga gagtatcttt ctattctcat aagttctgtt acttttacac
      241 taccagccct tgcaactctt ccaacatcat ttcttcctaa atcaacaagc ataagatgtt
      301 ctgctctctc ttttttatcg ttgattagtt cgttttccat ctctaaatct ttttctaaag
      361 tagaacctct ttttcttgtt cctgctattg gtcttaaaag taggtgacca tctactaatc
      421 taatcataac ctctgggcta cttcctgcaa tactaaaatc ttcaaattct aataaaaata
      481 ggtatggact aggattttta cttcttaatg ctctataaaa agataagcta tcaactactg
      541 ctttttgagt aaatctattt gagattaaaa tctggaatat atctccagct cttatcatct
      601 cttttgattt ttcaactata gaaaagaact cttctttagt aaagttaaat tttccattat
      661 ctatcaattt tgcttttttt agtggagtat aaatatatgg ttttaaaagc tctttttcta
      721 tattttcaat ctcatttttc aactcattca ttgaagtaag aataactaac tttgaagttt
      781 tatgagaaaa tgctaatatt atctttggtc ttattaagtc taagtcagga atatttaact
      841 catcttgtaa agagttcatc gaagctttta gtcttggttc aaactctttt cccatatcat
      901 aaccaacatt tccaataaac ccatcaatta gcccaattcc tagctctttt gatttctctt
      961 tatatatatt tttttcaaaa ttcttataat atttttttaa aaataataga ggattattat
     1021 ctactttttc tattgttcca atttcatttt taaaaaaaca ttcattgttt ttataccaaa
     1081 ttctctctct tgctccaatt attatatatg aaaaatttcc atcattactt gagcttatac
     1141 tactttcaaa caaaaaacta agctcttttg gataaagagc ttttgttttt tcataaatag
     1201 aaactggtgt aaattgatct aaaaatagag ttttactata aaaattcata aatatctact
     1261 aataatttac tataaaagta ttttgaatat ttaacttatt tttaacagct gatacatcat
     1321 ttgatgcagc tgctctacta ctataaggcc caataaggag tttgttaaat gtgctacctt
     1381 ttacatcttc ttgaataact tt
//
LOCUS       DAOQAN010000393         1400 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_147815_length_1400_cov_1.895911, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000393 DAOQAN010000000
VERSION     DAOQAN010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1400
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147815_length_1400_cov_1.895911"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1263)
                     /locus_tag="PKO10_06280"
     CDS             complement(<1..1263)
                     /locus_tag="PKO10_06280"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNW66790.1"
                     /translation="MSSKFFTNQGTNTLENRLKDILTHYDIKNLEFLIGYFRISGFKK
                     IAKLLDNVDKVRILVGINIDKLTHDAQARGKKFNLFDYEKFNQEFIENQKECLTNEEY
                     TQEVDESVNQFIKLIAQNRLEIRISQDKNIHSKIYILRENEIKRHNDTTEYRGSVITG
                     SSNLTENGLSKNYEFNVELKYSEDIEFALNEFNDLWIKSVEITEKDIDTIKQNSYLKE
                     ITPYELYLKFLIEHFEDRVDYDASVAEDLPKGYMKLAYQIDAVNEGLSKIKKHSGFFL
                     SDVVGLGKTITSAMIVKRLCYQTKGQILIIAPPSIEKEWNETFKEFQIGSIRKFDFKS
                     YGALEKIKDTEDYEIVIIDESHKFKNFLTSRYKELERICKEDVKYKKKVILISATPLN
                     NKPQDIANQLYLFQDKRNSTIDSHPNLET"
BASE COUNT          462 a          214 c          126 g          598 t
ORIGIN      
        1 agtctctaaa tttggatgag aatctattgt agaatttctt ttgtcttgaa aaagatacaa
       61 ttgatttgct atatcttgtg gcttattgtt taaaggtgtt gccgaaatta atataacttt
      121 tttcttatat ttaacatctt ctttacaaat tctttccaac tctttatatc ttgaagttaa
      181 aaaattttta aatttatgag attcatctat tatcactatc tcataatctt ctgtatcttt
      241 tattttttct aaagctccat aagatttaaa atcaaacttt ctaatacttc ctatttgaaa
      301 ctctttaaaa gtttcattcc actctttttc aatagatggt ggagcaataa taagtatttg
      361 tccttttgtt tgataacaca atcttttgac tatcatagca cttgtaattg tttttccaag
      421 accaacaaca tctgatagaa aaaaaccact atgttttttt atttttgata atccctcatt
      481 tacagcatct atttgataag ctaatttcat ataacctttt ggcaaatctt ctgcaacaga
      541 agcatcataa tcaactctat cttcaaaatg ttctattaaa aattttaaat atagttcata
      601 tggagttatc tcttttagat aactattttg ttttatggta tctatatctt tttctgtaat
      661 ctcaaccgat tttatccata aatcattaaa ttcatttaat gcaaactcta tatcttcact
      721 atattttaat tctacattaa actcataatt ctttgatagc ccattttctg ttagatttga
      781 acttcctgtt ataactgaac ctctatactc agtagtatca ttatgtcttt ttatctcatt
      841 ttctctaagt atgtatattt ttgaatgaat atttttatct tgagaaattc taatttccaa
      901 tctattttgt gcaattaatt ttataaactg atttacactc tcatcaactt cttgagtata
      961 ctcttcattt gtgagacact ccttttgatt ttctataaat tcttgattaa atttttcata
     1021 atcaaataga ttgaactttt tacctcttgc ttgagcatca tgagttaatt tatcaatatt
     1081 tattcctact aatatcctaa ctttatcaac attatcaagc agtttagcta tttttttaaa
     1141 tccactaatt ctaaaataac caattaaaaa ttctaaattt ttaatatcat agtgagtaag
     1201 tatatctttt aatctatttt ctaatgtatt tgtaccttga ttggtaaaaa attttgaact
     1261 catattataa tctatccatg tgaaatttat tttataaaag caagattgta ccctaaagag
     1321 ttttaaacca aaaagtaatt aaaaatatac ttactttata aaatatgtat gaaaaatatc
     1381 tgccaaaata tataacacta
//
LOCUS       DAOQAN010000394         1399 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_147997_length_1399_cov_1.893601, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000394 DAOQAN010000000
VERSION     DAOQAN010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1399
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147997_length_1399_cov_1.893601"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..912)
                     /locus_tag="PKO10_06285"
     CDS             complement(<1..912)
                     /locus_tag="PKO10_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNW66791.1"
                     /translation="MNDNIFEKMTNQLRETIDSSVALALHNKNQEVQIVHLIWALLTN
                     TNSVLNQLLNKMNVNKAAIELEAKSNASKLPSVSSVTKESIKLSRELYESFQKAQGQM
                     ASNGDKFIAIDTWLISNFDNKVIKDILGKYIDLKEAKKELETIRAGKQIDSQSGDDNL
                     EALNKYGIDLNKKAIDGELDPVIGRDEQINRMMQILIRKTKNNPILIGEPGTGKTAIA
                     EGLAQRIVNKDVPTSLLNKRVVTLDMSALIAGAKYRGEFEDRLKSVIDEVKQAGNIIL
                     FIDEIHTIIGAGASEGSMDAANILKPSL"
     gene            1034..>1399
                     /locus_tag="PKO10_06290"
     CDS             1034..>1399
                     /locus_tag="PKO10_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HNW66792.1"
                     /translation="MKNLCGIDEAGRGPLAGPLVVAGVILEKDILGLNDSKVLSEKKR
                     EKLFDEIKEKSKYHIVFKSAKEIDDFGISFCLKSSILEIMEKLQEFSDNFLMDGNTNF
                     GIENLQKEIKADAKYASVSA"
BASE COUNT          444 a          211 c          181 g          563 t
ORIGIN      
        1 caaactaggt tttaaaatat ttgcagcatc catagatcct tcactggcac ctgccccaat
       61 tattgtatga atttcatcta taaataagat tatatttcca gcttgtttaa cttcatctat
      121 tacagatttt aatctatctt caaactctcc tctatatttt gcacctgcta ttaaagcact
      181 catatcaaga gttacaactc ttttatttaa taaacttgta ggtacatctt tatttacaat
      241 tctttgagct aaaccttctg caattgctgt ttttccagtt cctggttctc ctattaaaat
      301 aggattgttt tttgtttttc taattaagat ttgcatcatt ctgtttatct gctcatctct
      361 tccaataaca ggatcaagct ctccatcaat agctttttta tttaaatcta tcccatattt
      421 atttaaagct tcaagattat catctccact ttggctatct atttgttttc ctgctcttat
      481 agtttctagc tctttttttg cttcttttaa atctatatat ttacctaaaa tatcttttat
      541 tactttatta tcaaaatttg aaataagcca agtatcaata gctataaact tatctccatt
      601 gcttgccatt tgcccttgtg ctttttgaaa actctcataa agttcacgtg aaagttttat
      661 actctctttt gtaacactgc ttacacttgg aagtttactt gcatttgatt ttgcttcaag
      721 ctcaattgca gctttattta cattcatttt atttaataat tggtttaaaa ctgaattagt
      781 atttgtaagc aatgcccaaa tcaagtgaac tatctgcacc tcttgatttt tattatgtaa
      841 agctaaagct acactactat ctattgtctc tcttaattga tttgtcattt tttcaaaaat
      901 attgtcattc atattagcac tcctagtatt taattgctaa tgttattata taactttagt
      961 caagtcttgt caagttttat ataaaatata ttttttatta taatactttt ctaagaaaaa
     1021 taaaaggttt tttgtgaaaa atttatgtgg aatagatgaa gcaggacgtg gaccgcttgc
     1081 tggtcctttg gtagttgctg gtgttatttt ggaaaaagat attttaggat taaatgattc
     1141 aaaagtattg agtgaaaaaa aaagagagaa actttttgat gaaataaaag agaaatcaaa
     1201 atatcatata gttttcaaaa gtgcaaaaga gatagatgat tttggtatta gtttttgtct
     1261 taaatcttca attttagaaa taatggaaaa attacaagaa tttagcgata attttttgat
     1321 ggatggaaat acaaattttg gtatagaaaa tctacaaaaa gagataaaag cagatgcaaa
     1381 atatgctagt gttagtgct
//
LOCUS       DAOQAN010000395         1398 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_148151_length_1398_cov_2.099777, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000395 DAOQAN010000000
VERSION     DAOQAN010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1398
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_148151_length_1398_cov_2.099777"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            147..1307
                     /locus_tag="PKO10_06295"
     CDS             147..1307
                     /locus_tag="PKO10_06295"
                     /inference="COORDINATES: protein motif:HMM:NF019421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HNW66793.1"
                     /translation="MQNNSKNGLKVFVNKNITGALNFENDTYIFNYKDDAKDIVSLTM
                     PIRSASWNSKKLHPIFQMNMPEGALKETIKNHFSKIETMTDINMLKLIGPYMLGRVKF
                     EEIKDVQDNLNLDDILNSSKQNLFDELLLKFAIKSGISGVQPKLLLKAFDKTTMKFEN
                     YIVKSWENNYPNLALNEYFCMKACSYAKLPTPEFYISDDFKMFVMKRFDILEDGTYLG
                     FEDMCVLTARGVENKYDGSYEECARVIKDIISPQYKKESLKIFFKSLVMNHFLKNGDA
                     HLKNYGILYKNDFNNSFLAPIYDVITTKVYIKNDIPALRLSDGKLWWKEKTYKNFAKL
                     SCNLTNVEYETIIDECSEAIRLTKNEILEFIKKYPKQKIFLDSLIECWSENL"
BASE COUNT          568 a          136 c          218 g          476 t
ORIGIN      
        1 aacaatctca aaaatagaaa atggctttat agatgaagtt ggaattaaaa aagttgaaaa
       61 tattttggat ttacttggag ttgaatttgc cattagacca aaaggaagac caaaaactct
      121 tgaagagtta caagaggagc tagaacttgc aaaataactc taaaaatggt ttgaaagttt
      181 ttgtaaataa aaatattaca ggagctttaa attttgaaaa tgatacatat atttttaatt
      241 ataaagatga tgcaaaagat attgtaagcc ttacaatgcc aataagaagt gctagctgga
      301 atagtaaaaa acttcatcca atatttcaaa tgaatatgcc agaaggtgct ttaaaagaga
      361 ctattaaaaa tcacttctca aaaatagaga ctatgactga tataaatatg ttaaaactaa
      421 ttggtccata tatgctaggt cgtgttaaat ttgaagagat taaagatgta caagataatc
      481 taaatttaga tgatatttta aactcttcaa aacaaaattt atttgatgaa cttcttctta
      541 aatttgctat aaaatctgga atttcaggtg ttcagccaaa gttactttta aaagcttttg
      601 ataaaacaac aatgaaattt gaaaattata tagttaaatc atgggaaaat aattacccaa
      661 atttagcttt aaatgaatat ttttgtatga aagcttgctc ttatgcaaaa cttccaactc
      721 cagagtttta tataagtgat gattttaaaa tgtttgttat gaaaagattt gatattttag
      781 aagatggaac atatcttgga tttgaggata tgtgtgtatt aactgcaaga ggagttgaaa
      841 ataaatatga tggaagctat gaagagtgtg ctagagttat aaaagatata ataagccccc
      901 aatataaaaa agagtctcta aaaatatttt ttaaatcttt agttatgaat cattttctta
      961 aaaatggtga tgctcattta aaaaattatg ggattttata taaaaatgat tttaataatt
     1021 catttttagc tccaatttat gatgttataa ctacaaaagt ttatattaaa aatgatattc
     1081 cagctttaag acttagtgat ggaaaacttt ggtggaaaga aaaaacttat aaaaactttg
     1141 caaagcttag ttgtaatctt acaaatgttg agtatgaaac tataatagat gagtgcagtg
     1201 aagcaataag attaacaaaa aatgaaatac tagagtttat aaaaaaatat ccaaagcaga
     1261 agatttttct agatagtttg attgagtgtt ggagtgaaaa tttataataa aattataggt
     1321 ttagcattaa ttttaatgga caaaaatgtg tctttattga ctgttataat tctttaatca
     1381 tttctttgaa cttaaaat
//
LOCUS       DAOQAN010000396         1394 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_148746_length_1394_cov_3.099328, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000396 DAOQAN010000000
VERSION     DAOQAN010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_148746_length_1394_cov_3.099328"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="PKO10_06300"
     CDS             <1..486
                     /locus_tag="PKO10_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014474991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HNW66794.1"
                     /translation="NGKSNAYNKDAFCEALKLVMHDMAMLMVADGEGAKKVAAFEVRN
                     AATKEDAIKAAKALSNSLLVKTALFGEDPNFGRIASTIGASQIACDDEKLVISYNDVV
                     VFNKGEICFDSIIEQKAADVLKKDQYKIICDIGLGDESFTAYGCDLGYKYVEINADYR
                     S"
     gene            541..756
                     /locus_tag="PKO10_06305"
     CDS             541..756
                     /locus_tag="PKO10_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HNW66795.1"
                     /translation="MLHEHRDAIAELRQKDAHFAKVFDKHNDLDHEITNLENSHADQF
                     TIETKKKEKLKLKDEIYSMIVKYKAEK"
     gene            901..>1394
                     /locus_tag="PKO10_06310"
     CDS             901..>1394
                     /locus_tag="PKO10_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014469167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNW66796.1"
                     /translation="MENLFENQDIVDINIEDSVKTSYLDYSMSVIIGRALPDAKDGLK
                     PVHRRILYAMHDLNLTSKVAYKKSARIVGDVIGKYHPHGDISVYDALVRMAQSFSMRA
                     PLVDGQGNFGSVDGDSAAAMRYTEARMTRIAEEVLRDLDKDTVNFVPNYDDTMKEPAV
                     LPTRV"
BASE COUNT          530 a          159 c          252 g          453 t
ORIGIN      
        1 aatggtaaat caaatgctta taataaagat gcattttgtg aagctctaaa attggtaatg
       61 catgatatgg ctatgttaat ggttgcagat ggtgaaggtg caaaaaaagt tgcagctttt
      121 gaagtaagaa atgcagcaac taaagaagat gcaattaaag cagcaaaagc tttatcaaat
      181 tctcttttag ttaaaacagc actttttggg gaagatccaa attttggaag aattgcttca
      241 acaattggag cttcacaaat agcttgtgat gatgaaaaat tagtaatttc ttataatgat
      301 gttgtagtat ttaataaagg tgaaatctgt tttgactcta taattgagca aaaagctgca
      361 gatgttctaa aaaaagatca atacaagatt atttgtgata ttggtttagg cgatgagagt
      421 tttacagcat atggttgtga tttgggctac aaatatgtag aaatcaatgc agattataga
      481 agttaatatt aagaactttt tatatataat ctcgtttaaa attttaaaca aggacggata
      541 atgcttcacg aacacagaga tgcaatcgca gaactaagac aaaaagatgc acattttgct
      601 aaagtttttg ataaacataa tgatttagat catgaaatca caaatttaga aaattcacat
      661 gctgaccaat ttactattga gacaaagaaa aaagagaaat tgaaacttaa agatgaaatt
      721 tactctatga tagtaaaata caaagctgaa aaatagtttt ataattatta atagttgtaa
      781 aaggaagtag agtttttctc tacttccttt ttttttacat ataaaactat tactaagtat
      841 aaattagata gaataaaaac aattttttta ataaaaactt tatattaaag gccttatttt
      901 atggaaaacc tttttgagaa ccaagatatt gtagatataa atattgagga tagtgtaaaa
      961 acttcatatt tagattactc tatgagtgtt attattggac gagcattacc agatgcaaaa
     1021 gatggattaa aacctgttca tagaagaatt ttatatgcaa tgcatgattt gaatttaact
     1081 tcaaaagttg cttataaaaa atctgcaaga attgtcggag atgttatagg aaagtaccat
     1141 ccacatggag atatttctgt atatgatgca cttgtaagaa tggctcaaag tttctctatg
     1201 agagcacctt tggttgatgg acaaggaaac tttggttctg ttgatggcga tagtgcagct
     1261 gctatgagat atacagaagc tagaatgaca agaattgctg aagaagtttt aagagatttg
     1321 gataaagata ctgtaaattt tgttcctaac tatgatgaca ctatgaaaga acctgctgtt
     1381 cttcctacaa gagt
//
LOCUS       DAOQAN010000397         1392 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_149101_length_1392_cov_2.575168, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000397 DAOQAN010000000
VERSION     DAOQAN010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149101_length_1392_cov_2.575168"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="PKO10_06315"
     CDS             <1..368
                     /locus_tag="PKO10_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HNW66797.1"
                     /translation="FDIYAKTRDEECEKILPIKRYTDSFLIHPNDVLKADKTPYKVFT
                     AFYNSLEFLHSSNNIKEFETPKNLKKSKFDYSFIPTLNDLGLKKQDLPDFLYKSADEL
                     IDIFSKKIEIIKRIETIFI"
     gene            515..1069
                     /locus_tag="PKO10_06320"
     CDS             515..1069
                     /locus_tag="PKO10_06320"
                     /inference="COORDINATES: protein motif:HMM:NF015406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding domain-containing protein"
                     /protein_id="HNW66798.1"
                     /translation="MLYHFPKSQFENLNSIKVNWNQNEDDFKNWCEGNTGVPIIDASM
                     RYLNQTGLMHNRLRMVTSSYLTKNLLIDWRKGEEYFALKLLDYEASSNIGSWQWAAST
                     GVDAVPYFRVFNPYLQSKKFDKDAIFIKTVLKELKDIDAKVIHTENGVQEDIFLNYPR
                     QIVGINYSRNRAIMEFKRANSFNK"
     gene            complement(1103..>1392)
                     /locus_tag="PKO10_06325"
     CDS             complement(1103..>1392)
                     /locus_tag="PKO10_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66799.1"
                     /translation="EKFGYQKFGIHKNYYADELFIKIVENNDEYLENGFLNPMRCNNT
                     NSIEVSIYENNTDLVIISRLDNLGKGASGAAIQCMNIMLGLEERKGLEIKL"
BASE COUNT          524 a          163 c          205 g          500 t
ORIGIN      
        1 atttcgatat ttatgcaaaa acaagagatg aagagtgtga gaaaatattg ccaataaaaa
       61 gatatacaga ctctttttta atccatccaa atgatgtatt aaaagctgat aaaacaccat
      121 acaaggtatt tacagctttt tataactctt tggaattttt gcatagttca aacaatataa
      181 aagagtttga aactcctaaa aatttaaaaa aatcaaagtt tgattatagt tttattccta
      241 ctttaaatga tttaggatta aaaaagcaag atttaccaga ttttttatat aaaagtgcag
      301 atgaattaat agatattttt tcaaaaaaaa ttgaaattat caagagaata gagactattt
      361 ttatttagat gctagctcaa atttaagtgt tcatttaaga tttggtctta tttctcctag
      421 aacactattt aataaaataa aaaggctaaa tgcaccaaaa aagcagatag atttttttat
      481 aagagagcta ttttggagag agttttataa ctatatttta taccatttcc caaaatcaca
      541 atttgaaaat ctaaattcaa ttaaagtaaa ttggaatcaa aatgaagatg attttaaaaa
      601 ttggtgtgaa ggaaatacgg gtgttccaat aattgatgct agtatgagat atttaaatca
      661 aacaggactt atgcataatc gtttaagaat ggttacctct tcatatttga caaaaaattt
      721 attaatagat tggagaaaag gtgaagagta ttttgctttg aaacttttgg attatgaagc
      781 aagttcaaat ataggctctt ggcagtgggc tgctagtact ggagttgatg cagttccata
      841 ttttagagtt tttaatccat atttacaatc aaaaaaattt gataaagatg ctatttttat
      901 aaaaactgtt ttaaaagagc taaaagatat tgatgctaaa gtaatacata cagaaaatgg
      961 tgttcaagaa gatatttttc taaattatcc aagacaaatt gtaggtatta actattcaag
     1021 aaatagggct attatggagt ttaaaagagc gaatagtttt aataaataat aaatcttagc
     1081 tagtagggtg aggataagaa gtttagagtt ttatctccaa accttttctc tcttcaagtc
     1141 ctagcatgat attcatacat tgaatagctg ctccacttgc accttttcct agattatcaa
     1201 gtcttgatat gattactaaa tctgtgttgt tttcataaat tgatacttct atactatttg
     1261 tattgttaca tctcattgga tttagaaaac cattttctag gtattcatca ttgttttcaa
     1321 ctatttttat aaaaagttca tcagcataat aatttttatg aataccaaat ttttggtaac
     1381 caaatttttc gt
//
LOCUS       DAOQAN010000398         1392 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_149185_length_1392_cov_1.540015, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000398 DAOQAN010000000
VERSION     DAOQAN010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149185_length_1392_cov_1.540015"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..499)
                     /locus_tag="PKO10_06330"
     CDS             complement(20..499)
                     /locus_tag="PKO10_06330"
                     /inference="COORDINATES: protein motif:HMM:NF027551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4885 family protein"
                     /protein_id="HNW66800.1"
                     /translation="MNINTNIQSFSSQSGIKVTQNGNTNQSIDKSKNSEAVNINIDSK
                     TLDTLNLLSSLEEETFIKSYNNFISDERRAIRNRLEEVYSKATKENKKFANPEQHIRD
                     KYFSQASPYYIEGLSRMEREIAYNHEMDYLKNNKLTKINYIDPNLKDLTSIHGCPYL"
     gene            615..1241
                     /locus_tag="PKO10_06335"
     CDS             615..1241
                     /locus_tag="PKO10_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66801.1"
                     /translation="MEIGKATSVYQTQEVKGTKETYYKEAQKVDYSKYSADDLMQIPF
                     EEAKLNQESIDKRYQELADDGNIKPRHAVGLLALKGALNFSGNSSIDKAYYQTLQSSP
                     KENLGLVTYEFQMNFQGFAQGEDISPTFMKDGRSGNGSYLLNKNQATSVDFDAFINSA
                     IASFEKNLTKAKSSNAENSVQNQYQGIVDFYKTFQDNFNKATKEPYYA"
BASE COUNT          528 a          168 c          197 g          499 t
ORIGIN      
        1 gacggtatca accaagagtt taaaggtaag ggcaaccatg aattgaagtt aaatctttta
       61 gatttgggtc tatgtaatta attttagtta gtttattatt ttttaaataa tccatttcgt
      121 ggttgtatgc aatttctctt tccattctac ttaaaccttc tatataatat ggtgaagctt
      181 gtgagaaata tttatctcta atatgttgct ctggatttgc aaatttttta ttctcttttg
      241 ttgcttttga gtaaacttct tctagtctat ttctaatagc tcttctctca tcacttataa
      301 agttattgta gcttttaata aaagtttctt cctccaaaga tgataataga tttaaggtat
      361 ctaatgtttt tgagtctata tttatattta cagcttcact atttttactt ttatctatac
      421 tctgattagt atttccattt tgtgttactt ttattccact ttgtgaacta aacgattgta
      481 tattagtatt tatattcata agaatcccct tatttattac taacttttct taatattagt
      541 ttaattataa tcttttaaac tttatttaac tttaatacta ataaaataaa acattattat
      601 acaaggggta aaaaatggaa ataggtaaag caacaagtgt atatcaaaca caagaggtaa
      661 aaggaacaaa agagacttat tataaagaag cacaaaaagt tgattatagt aaatattcag
      721 cagatgattt aatgcaaata ccttttgaag aggcaaaatt aaatcaagaa tctatagata
      781 aaagatacca agagttagct gatgatggaa acataaaacc tagacatgct gttggacttt
      841 tagctttaaa aggagcttta aattttagtg gaaacagtag tattgacaaa gcatactatc
      901 aaactttaca aagttctcca aaagaaaatc ttggtcttgt aacatatgaa tttcaaatga
      961 attttcaagg atttgcacaa ggagaagata taagcccaac ttttatgaaa gatggaagat
     1021 ctggaaatgg aagttattta ctaaataaaa atcaagctac aagtgtggat tttgatgcat
     1081 ttataaatag tgcaattgca agttttgaga agaacttaac aaaagcaaaa tcttcaaatg
     1141 cagaaaatag tgtacaaaat caatatcaag gaatagttga tttttataaa acttttcaag
     1201 ataattttaa taaagctaca aaagagccat attatgcttg aatttaaaat caagtagttt
     1261 ttaataaata tttaaattag gggaaaatat atgcaaatag gaaataacca aactcaaagc
     1321 tatggatata atcaaaacgt aaatgtagcg aataaaaatg atagttcaaa ctcttttaat
     1381 aattatcttg aa
//
LOCUS       DAOQAN010000399         1389 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_149646_length_1389_cov_2.395802, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000399 DAOQAN010000000
VERSION     DAOQAN010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149646_length_1389_cov_2.395802"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1389
                     /locus_tag="PKO10_06340"
     CDS             <1..>1389
                     /locus_tag="PKO10_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HNW66802.1"
                     /translation="IQLPVILLLSNENSCILDSFNEDRTKAKIIFAGIDDPLEEWVEV
                     QKLEDEYLGYAFMLKKVYEYEHDNGKKTLAIDNQKHWFWSTLGVSKKIYIDCIIASIL
                     INLFVLATPLFTMNVYDRVIPNNAQETLLVFTIGIVVVFILDAILKFIRTYFLEMAGK
                     KSDIIMSSIIFEKVLDMKLSEHPKSVGSFANNLKSFDSIRGFLTSSTLTVLIDFPFAI
                     LFLIVIGYLGGFLVIVPIFIIMLIIIYAKIIKDPLKKSIEATYEASAKKNGILIESLN
                     NIETIKAQGMAGNVQYSWEESTGEIANKGLKSKLISASIPTVTGLLTGLNTVLIVVFG
                     VYLIQDFQLTMGGLIATMILSGRAIAPMGQIVSLITNYEDTKQSFKMLDDIINKPQER
                     PFSKEFVKKTNLSGNIEFRNVSFRYPNSESYALRNVSFTIKEGEKVAFIGRIGSGKST
                     IAKLILKLYEPE"
BASE COUNT          500 a          150 c          237 g          502 t
ORIGIN      
        1 atatacaact tcctgtaata ctacttttat caaatgaaaa tagttgtatt ttggactctt
       61 tcaatgaaga tagaacaaaa gcaaagatta tttttgctgg aattgatgat cctcttgaag
      121 agtgggttga ggtgcaaaag cttgaagatg aatatttagg ttatgctttt atgctcaaaa
      181 aagtttatga atatgagcat gataatggta aaaagacttt agcaattgat aatcaaaaac
      241 attggttttg gagtacttta ggagtttcaa aaaagattta tattgattgt ataatagctt
      301 cgattttaat aaaccttttt gtactcgcaa ctccactttt tactatgaat gtttatgata
      361 gggttattcc aaataatgca caagagacac ttcttgtttt tactattggt attgttgttg
      421 tatttattct tgatgccatt ttaaagttta ttagaaccta ttttctagaa atggctggga
      481 aaaagagtga tattataatg tcttcaatta tttttgaaaa agttttagat atgaagttaa
      541 gtgaacaccc aaaatctgtt gggagttttg caaataatct taagagcttt gatagtataa
      601 gagggttttt aacaagttct actttgactg tattaattga ttttccattt gctattttat
      661 ttttaatagt aattggatat ttaggtggtt ttttggtgat agttccaatt tttattatta
      721 tgttaatcat aatttatgca aaaataataa aagacccact taaaaaaagt attgaagcaa
      781 cttatgaagc aagtgcaaag aaaaatggta ttttaataga gagcttaaat aatattgaaa
      841 caataaaagc tcaaggaatg gctggaaatg ttcagtactc ttgggaagaa tcaactggtg
      901 aaatagcaaa taaaggtctt aaatcaaaac taatatctgc ttctattcca actgttacag
      961 gtcttttaac tggtttaaat acagttttaa ttgttgtttt tggagtttat ttaattcaag
     1021 attttcaact tacaatggga ggtttaattg ctactatgat tttatcaggt cgtgcaattg
     1081 cacctatggg gcaaatagtt tcactaatta caaactatga agatactaaa caatctttta
     1141 aaatgttgga tgatattata aataaaccac aagagagacc attttctaaa gagtttgtta
     1201 aaaaaacaaa tttaagtgga aatattgagt ttagaaatgt aagttttaga tacccaaata
     1261 gtgaatcata tgctttaaga aatgtaagtt ttactataaa agagggtgaa aaagttgctt
     1321 ttattggaag aattggaagc ggaaaatcaa ctattgcaaa acttatttta aaactatatg
     1381 aaccagaag
//
LOCUS       DAOQAN010000400         1388 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_149847_length_1388_cov_2.042761, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000400 DAOQAN010000000
VERSION     DAOQAN010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1388
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149847_length_1388_cov_2.042761"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..>324)
                     /locus_tag="PKO10_06345"
     CDS             complement(133..>324)
                     /locus_tag="PKO10_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HNW66803.1"
                     /translation="RPYNKKLKVAQLLILIGTLIFSISLYLLTILNLPILGAITPIGG
                     TLQIIAYVLLAYAIFKDDK"
     assembly_gap    325..334
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<335..525)
                     /locus_tag="PKO10_06350"
     CDS             complement(<335..525)
                     /locus_tag="PKO10_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HNW66804.1"
                     /translation="MIFDRRARIFLAITALLMASAIAFGAFGAHGLKNILTPEMLKVF
                     HTGVEYQFYNTFGLFMATV"
     gene            complement(528..>834)
                     /locus_tag="PKO10_06355"
     CDS             complement(528..>834)
                     /locus_tag="PKO10_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HNW66805.1"
                     /translation="VFTINSISYDRKNQKDSIEIFNIPDLKSYKKVLIIDDIVDSGKT
                     MIEVFKILNEKFPNTEFKLATLFYKKTALIKPDFYIKETDMWIEFFWEVDIQIKEER"
     assembly_gap    835..844
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<845..976)
                     /locus_tag="PKO10_06360"
     CDS             complement(<845..976)
                     /locus_tag="PKO10_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002869334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HNW66806.1"
                     /translation="MCKEDTQKLVDISKSFKADAFLSIARGGLTLSHLMSQALNQRNV
                     "
     gene            complement(1117..>1388)
                     /locus_tag="PKO10_06365"
     CDS             complement(1117..>1388)
                     /locus_tag="PKO10_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitate hydratase B"
                     /protein_id="HNW66807.1"
                     /translation="NQAQVGEGSTVFSTSTRNFDNRLGKNSKVYLGSAEVAAVAALLG
                     RLPSVQEYLDIVAKKINESNKDGVYKYLNFHQVTSDHLTTLLTSR"
BASE COUNT          482 a          203 c          161 g          522 t
ORIGIN      
        1 tttattttct ccttttttaa tagttcctat taaaaaagga accgtttctt ttatttctac
       61 tttttatagc gtggatttaa aatcccctaa aaagtagcaa aaacctaaag tgctttgaga
      121 acaaagcatt acttatttat catctttaaa tattgcataa gccaaaagaa catatgcaat
      181 aatttgtaaa gttccaccta ttggtgtaat tgcacctaaa attggaagat ttaaaattgt
      241 aagaagatat aaagatatac taaaaataag tgttccaatt aaaattaata gttgagcaac
      301 ttttaatttt ttattgtatg gtctnnnnnn nnnnaaaaca gttgccataa acaatccaaa
      361 agtgttataa aactgatatt caacgcctgt atgaaatact ttcaacatct ctggagttaa
      421 aatatttttt aatccatgag ctccaaaagc tccaaaagct attgcacttg ccataagtaa
      481 agctgtaatt gctaaaaaaa ttctagctcg tctatcaaaa atcatatcta tctctcttct
      541 ttaatttgta tatcaacttc ccaaaaaaac tcaatccaca tatccgtctc ttttatataa
      601 aaatctggtt ttatcaaagc tgttttttta taaaaaagtg tagccaattt aaactctgta
      661 tttggaaatt tttcatttaa tattttaaaa acttctatca tagttttacc actatcaact
      721 atatcatcaa taatcaaaac ttttttataa gattttaagt ctggaatatt aaaaatctct
      781 atactatctt tttgattttt tctatcataa gatattgaat ttatagtaaa aacannnnnn
      841 nnnnaacatt tctttgatta agtgcttgtg acataagatg agatagagtt aatccacctc
      901 ttgcaatact taaaaatgca tccgctttga aacttttaga aatatctact agtttttgag
      961 tatcttcttt acacatttca taactataat ataatttatt caaaaaaata tcctttattt
     1021 aaaaaagata tattaacaat ttattgctta ttttgtggtt ttgtgagttt aatttatata
     1081 aaaagaggaa ccctcttttt atatatttat attttcttat cttgaagtta aaagtgtagt
     1141 taagtgatca gatgtaactt ggtggaagtt taagtatttg taaactccat ctttgttgct
     1201 ttcattgatt tttttagcaa cgatatctaa atactcttga acagatggta atcttcctaa
     1261 aagtgcagca acagcagcaa cttcagcaga acctaaataa actttagagt ttttaccaag
     1321 tctgttatca aagtttcttg tagatgtaga gaatactgtt gaaccttctc caacttgtgc
     1381 ttggttac
//
LOCUS       DAOQAN010000401         1387 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_149947_length_1387_cov_3.302553, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000401 DAOQAN010000000
VERSION     DAOQAN010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149947_length_1387_cov_3.302553"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="PKO10_06370"
     CDS             <1..286
                     /locus_tag="PKO10_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014473053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HNW66808.1"
                     /translation="RNNFTIRIDKSGLGEVDWNNPSINKGVDIGDNVKIADKPVIDIN
                     NAMINTKKEPTVSDLVRSMKVMKVPMNEIIDTLRMIKEMGAIDVDIELRG"
     gene            293..>1387
                     /gene="fliM"
                     /locus_tag="PKO10_06375"
     CDS             293..>1387
                     /gene="fliM"
                     /locus_tag="PKO10_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014473052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HNW66809.1"
                     /translation="MAEFLSQDEIDALLDIAEQGDDIDGTNPLDKFTAKEKNYSIYDF
                     KKPNRVTLDQLKALTTMHDKMLREFTNDLSSMLRKVVDVKLTSIEQMTYGEFILSIPQ
                     VTSLSTLSMKPLDGRIVIECNPTISHKVIADLLGSGAVNTMDNLDRELTEIEIKVLEH
                     FYKMFIKILYKTWSDVSSLNFKIESSDTNANAIQIVSDHDIVLLVVFEITIDEDSGFL
                     SICYPISYIEPLLNKIVDKIFSEGKNKKLSRKQDIKTLISGARMKIEPIMAETEMTTL
                     EILNLKEGDVIVFNKNAASSDSIVYVNKKEKFSAVCGISNNRKAIEIRSNLDKEKQET
                     LETLRLMREEREQKAKENAENIKKLINERRN"
BASE COUNT          570 a          150 c          240 g          427 t
ORIGIN      
        1 aagaaataac ttcacaataa gaattgataa atcaggatta ggagaagtag attggaataa
       61 tccatctata aacaaaggtg ttgatattgg tgataatgta aaaattgctg ataaaccagt
      121 aattgatata aacaatgcca tgataaatac aaaaaaagag ccaactgtat ctgatttagt
      181 tcgttctatg aaggtaatga aagttcctat gaatgagatt atagataccc ttagaatgat
      241 taaagagatg ggtgcgattg atgttgatat tgaattaaga ggataatgaa ctatggcaga
      301 atttttaagt caagatgaga tagatgctct tttagatata gctgagcaag gtgatgatat
      361 tgatggtaca aatccacttg ataaatttac agctaaagag aaaaattact ctatatatga
      421 cttcaaaaaa ccaaatagag taacactaga ccaactaaaa gctcttacaa caatgcatga
      481 taagatgtta agagagttta caaatgattt aagttcgatg cttagaaaag ttgtagatgt
      541 aaaacttaca tcaattgaac agatgactta tggtgagttt attttatcta ttcctcaagt
      601 tacatcactt agtacactat ctatgaaacc tcttgatgga agaatagtaa tcgagtgtaa
      661 ccctacaatt tcacataaag taatagctga tttgctagga agtggtgctg taaacactat
      721 ggataatctt gatagagaat taacagaaat agagataaaa gttcttgaac atttttataa
      781 aatgtttatt aaaattttat ataaaacttg gagtgatgtt tctagtttaa attttaaaat
      841 agaatcaagt gatacaaatg caaatgcgat tcaaatagtt tcagaccacg atatagtttt
      901 acttgttgtt tttgaaataa ctattgatga ggactcagga tttttatcta tttgttaccc
      961 tatttcttat attgaacctt tactaaataa aattgttgat aaaattttta gtgaaggtaa
     1021 aaataaaaaa ctaagccgta aacaagatat aaaaactcta atttcaggtg ccagaatgaa
     1081 aatagaacca attatggcag agacagaaat gactacttta gaaattttaa atctaaaaga
     1141 gggagatgta attgtattta ataaaaatgc tgcttcatct gatagtatag tttatgtaaa
     1201 taaaaaagag aaattttctg ctgtttgtgg tatttcaaac aatagaaaag caattgagat
     1261 tagatcaaat cttgataaag agaaacaaga aactcttgaa actttaagat tgatgagaga
     1321 agagagagaa caaaaggcaa aagagaatgc tgaaaatatt aaaaaattaa taaatgaaag
     1381 aagaaat
//
LOCUS       DAOQAN010000402         1387 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_149966_length_1387_cov_2.791291, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000402 DAOQAN010000000
VERSION     DAOQAN010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149966_length_1387_cov_2.791291"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1387
                     /locus_tag="PKO10_06380"
     CDS             <1..>1387
                     /locus_tag="PKO10_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014469361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNW66810.1"
                     /translation="EENISVKLHTTKNIENIKFLNLDFEFIEKEIKPFWNKVCTKERF
                     IQITDFLKNLEQNLELLEKYDEIVYKLNFVLFSQNFNLKLKDIYKIFLQKLAKISLDD
                     AHSGKITVMGLLETRLIDFDAIIICDFNNSFIPKISLKDKFLSTKVKYLANLPTKIDR
                     ENLQKYYYKRLIDSSKNIFISYVESKEDDISKFAYELFSDIKISTNDDEYKDILYKSR
                     KLEYKDEEIFLNIDLTKQTWSASGLKMFLECKRKWYLNYILKLKEHTISKLPKSFELG
                     NIVHSILEEYYKNKNSNIDEIFLKYKSSNPFLILDLEVFKHNIKKLLEDDKKRLETRE
                     IVEIEKKFSVNFNEFELIGVIDRVDKFEDSYELIDYKTSRSLKIDTINTYSKSCDFQL
                     EFYFLAIKNLYKTTNIKAFYYDIFENSLKQEVVLNEKLELLQNIFDDLKNQSKSKIDF
                     CKTEDKAICTYC"
BASE COUNT          605 a          114 c          183 g          485 t
ORIGIN      
        1 agaagaaaat atttcagtaa aattacatac tacaaaaaat atagaaaata taaagttttt
       61 gaatttggat tttgaattta tagaaaaaga gattaaacct ttttggaata aagtttgtac
      121 aaaagagaga tttatacaaa taacagattt tttaaaaaat cttgagcaaa atttagagct
      181 actagaaaaa tatgatgaaa tagtatataa attaaatttt gtattatttt cacaaaattt
      241 taatctaaaa ctaaaagata tttataaaat ctttcttcaa aaattagcaa aaatatcttt
      301 agatgatgca cattcaggaa aaataacagt tatgggactt cttgaaacac ggcttataga
      361 ttttgatgct attattattt gtgattttaa taactcattt attccaaaaa tttcattaaa
      421 agataaattt ttatctacaa aagtaaaata tttagcaaat ttaccaacaa aaattgatag
      481 agagaacctt caaaaatatt attataaaag attgatagac tcttcaaaga atatatttat
      541 ctcttatgtt gaatcaaaag aagatgatat ttcaaagttt gcttatgagt tattttctga
      601 tataaaaatc tcaacaaatg atgatgaata caaagatatt ttatacaaga gcagaaagct
      661 tgaatataaa gatgaagaga tttttttaaa tattgatttg acaaaacaaa cttggtcagc
      721 aagtggctta aaaatgtttt tagagtgcaa aagaaagtgg tatttaaact atattttaaa
      781 actcaaagaa catacaattt ctaaacttcc gaagagtttt gaacttggta atatagtaca
      841 tagtattctt gaagagtact ataaaaataa gaattcaaat atagatgaaa tttttttaaa
      901 atataaaagt agcaatccat ttttaattct tgatttagaa gtttttaaac ataacattaa
      961 aaaactttta gaagatgata aaaagagatt agaaactaga gaaatagttg agattgaaaa
     1021 gaaattttct gtaaatttta atgaatttga attaattgga gttatagata gagttgataa
     1081 atttgaagat agttatgagc taatagatta taaaacttca agaagcttga aaatagatac
     1141 aataaataca tatagtaaat cttgtgattt tcaattggag ttttattttt tagcaataaa
     1201 aaatttgtat aaaacgacaa atataaaagc cttttactat gatatttttg aaaatagttt
     1261 gaagcaagaa gttgttttaa atgaaaaatt ggaactttta caaaatattt ttgatgattt
     1321 aaaaaatcaa agcaaaagta aaattgattt ttgtaaaact gaagataaag ctatttgtac
     1381 ttattgt
//
LOCUS       DAOQAN010000403         1386 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_150162_length_1386_cov_2.522164, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000403 DAOQAN010000000
VERSION     DAOQAN010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1386
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150162_length_1386_cov_2.522164"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="PKO10_06385"
     CDS             <1..229
                     /locus_tag="PKO10_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HNW66811.1"
                     /translation="GSMFGFFFCEKEPKNFKEVGLCNFERFATFHREMLKRGFYFACS
                     QYEAGFICTKITNKMIDDCIKAADEVMKGLK"
     gene            229..543
                     /locus_tag="PKO10_06390"
     CDS             229..543
                     /locus_tag="PKO10_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HNW66812.1"
                     /translation="MESFKNVELIKKANIYFDGNVTSRTFIDIDGSKKSLGIMMIGEY
                     LFGTAEAEIMEIIDGHVEVRLKGESEWKSYKSGSSFEVPANSSFDIKVLTISDYCCSY
                     IK"
     gene            555..872
                     /locus_tag="PKO10_06395"
     CDS             555..872
                     /locus_tag="PKO10_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006655856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HNW66813.1"
                     /translation="MEDIKEKEQKPKHRDKIEALDSLSVGISMVAAIAIGVGIGLALK
                     HFTGYTWTLWLGIFWGIAAAGLNVYKAYKRAQKVYEGMENDPRYSYRAKHGDKSFDDD
                     EDK"
     gene            835..>1386
                     /locus_tag="PKO10_06400"
     CDS             835..>1386
                     /locus_tag="PKO10_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66814.1"
                     /translation="MVINHLMMMKINSEILNFAKVFLFLNLCLSLYAIFFENTIWLLN
                     LQIAFFASLLVTLASFLSYKKNIQNRLENLDKNHISSSEERDKIDEIDDPFDLYSEYK
                     EVPESELTPEKIKEIIDEEKSRVKQNVVKNTLFSATGFLSIYRIFGYGFLIFGFFALN
                     NNKILIPLAFIIGLSIVPIGVLFT"
BASE COUNT          516 a          122 c          265 g          483 t
ORIGIN      
        1 aggaagtatg tttggatttt tcttttgtga aaaagagcca aaaaacttca aagaagtagg
       61 gctttgtaat tttgaaagat tcgcaacttt tcatagagag atgttaaaaa gaggttttta
      121 ttttgcttgt agtcaatatg aagctggatt tatttgtaca aaaattacaa ataagatgat
      181 tgatgattgt ataaaagcag cagatgaagt tatgaaaggt ttaaaataat ggaaagtttt
      241 aaaaatgtag agcttatcaa aaaagcaaat atctattttg atggaaatgt aacaagtaga
      301 acttttatag atatagatgg aagtaaaaaa tctttaggaa ttatgatgat aggagagtat
      361 ctttttggta ctgctgaagc tgaaattatg gaaattatag atggtcatgt tgaagtaaga
      421 ttaaaaggtg aaagtgagtg gaaaagttat aaaagtggta gctcttttga agttccagcg
      481 aactcaagtt ttgatataaa agtattaaca ataagtgatt attgttgtag ttacataaaa
      541 tagattggaa ataaatggaa gatataaaag agaaagagca aaaaccaaaa catagagata
      601 agatagaggc acttgatagt ttatctgttg gtatttctat ggttgctgct attgcaattg
      661 gagttggaat aggtttagcc ctaaaacact ttacaggata tacatggaca ctttggcttg
      721 gtattttttg gggtattgca gcagctggtt taaatgttta caaagcttat aaaagagctc
      781 aaaaagtgta tgaaggtatg gaaaatgatc caaggtattc atatagagca aaacatggtg
      841 ataaatcatt tgatgatgat gaagataaat agtgaaattt taaactttgc aaaggttttt
      901 ttatttttaa atctttgtct tagtttatat gcaatatttt ttgaaaatac aatttggctt
      961 ttaaatctcc aaatagcctt ttttgcttcg cttcttgtaa ctcttgcttc atttttatct
     1021 tataaaaaaa atattcaaaa tagattagaa aatcttgata aaaatcatat ttcaagtagt
     1081 gaagagagag ataaaattga tgaaatagat gacccttttg atttgtatag tgagtataaa
     1141 gaggttcctg aaagtgaact aacaccagaa aaaataaaag agattataga tgaagaaaaa
     1201 agtagggtaa agcaaaatgt agtaaaaaat acactgttta gtgcaacagg ttttttatct
     1261 atttatagga tttttggtta tggattttta atttttggat tttttgcatt aaataataat
     1321 aaaattctta ttcctcttgc ttttataatt ggtcttagta ttgtaccaat tggagtttta
     1381 tttacg
//
LOCUS       DAOQAN010000404         1382 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_150857_length_1382_cov_2.032404, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000404 DAOQAN010000000
VERSION     DAOQAN010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150857_length_1382_cov_2.032404"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="PKO10_06405"
     CDS             <1..609
                     /locus_tag="PKO10_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008334937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNW66815.1"
                     /translation="WQRSAAWVTGVAAMLLIWLTFDSMSQIKMGSKEDLSSGITKRVP
                     SPTVINYKVSYEFSEKRGHEVPVIGEKEKFFGRDDYSEEEAMALLNLGKLASQTKNCM
                     NCHTLLGNGAYYAPDLTKAWLDPMWDSYMLRMGKASKEEAIAEFLQHPSDNPSNERMM
                     PNLGITADEAKGLVAFLKHMSSIDTNGFPRNFGKIEGGIHGR"
     gene            599..>1382
                     /locus_tag="PKO10_06410"
     CDS             599..>1382
                     /locus_tag="PKO10_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013554499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNW66816.1"
                     /translation="MVDNMKSQSKQLGKMYFTVAAILFGAQILFGLIAGTQFVYSGFL
                     FELLDFNVARMVHINALVVWLLYAMIGSVYYLLPDETKIETVGIGLGKLGFYVLTLAV
                     TVVVLVYILVQVGPANELSIWLIHEGREYIEAPRWADIGIVVVVLIFLANVYLTAIKG
                     ERTGIITVLMADLLALAGLYLAGMFYTNNISIDQYWWWWVIHLWVEATWEVFVASVIG
                     WALIRIIGANRQIVEMWLWIEVAMLFGSGILGIGHHYFWIGTP"
BASE COUNT          437 a          170 c          299 g          476 t
ORIGIN      
        1 tggcaaagat cagctgcttg ggttactggt gtagctgcca tgttactaat ttggctaact
       61 tttgacagta tgagtcaaat taaaatgggt tcgaaagagg atcttagtag tggaataaca
      121 aaaagagttc catctccaac tgttataaac tataaagttt cttatgaatt tagcgaaaaa
      181 agaggtcatg aagtacctgt aattggagaa aaagagaaat tctttggaag agatgattac
      241 agcgaggaag aagctatggc tttattaaat ttgggaaaat tagcttctca aactaaaaac
      301 tgtatgaatt gtcatacttt acttggaaat ggtgcatact atgctcctga tttaactaaa
      361 gcttggcttg atcctatgtg ggattcatat atgcttagaa tgggaaaagc ttcaaaagaa
      421 gaggctatag ctgaattttt acaacatcca tctgataatc catcaaatga gagaatgatg
      481 ccaaatcttg gtatcactgc agatgaagca aaaggtttag tggcattttt aaaacatatg
      541 tcatcaatag atacaaatgg tttccctaga aactttggga aaatcgaagg aggaattcat
      601 ggtagataat atgaaatctc aatctaaaca gttaggaaaa atgtatttta cagttgcagc
      661 aatacttttt ggtgcacaaa tactatttgg tctaattgct gggactcagt ttgtatatag
      721 tggattttta tttgaactat tggatttcaa tgttgctaga atggtacaca taaatgcttt
      781 agttgtttgg cttttatatg caatgattgg ttctgtttac tatttacttc ctgatgagac
      841 aaaaatagaa acagttggaa ttggtttggg aaaacttgga ttttatgtat taactttagc
      901 tgttacagtt gtagtattgg tatatatttt agtacaagta ggacctgcaa atgagctatc
      961 tatttggcta attcacgaag gaagagagta tattgaagct cctagatggg ctgatattgg
     1021 tatagttgtt gttgttttaa tctttttagc aaatgtttat ttaactgcaa taaaaggtga
     1081 aagaacagga attattacag ttttaatggc tgacttatta gcacttgctg gattatattt
     1141 agctggaatg ttttatacaa ataatatctc tattgatcaa tactggtggt ggtgggttat
     1201 tcacctttgg gttgaagcta cttgggaagt atttgttgcc tctgttattg gttgggcgtt
     1261 gattagaata attggtgcaa atagacaaat tgttgaaatg tggttatgga ttgaagtagc
     1321 aatgctattt ggttctggaa ttttgggaat cggtcaccac tatttctgga ttggaacacc
     1381 tg
//
LOCUS       DAOQAN010000405         1381 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_150957_length_1381_cov_2.541478, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000405 DAOQAN010000000
VERSION     DAOQAN010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1381
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150957_length_1381_cov_2.541478"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="PKO10_06415"
     CDS             complement(<1..184)
                     /locus_tag="PKO10_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family peptidase"
                     /protein_id="HNW66817.1"
                     /translation="MRNRVLFLLLLSLNLFANENLAKRLILAYPLFLEKYEDNFIYFK
                     DGSKLQFSTNNKDLSYE"
     gene            complement(174..1250)
                     /gene="aroC"
                     /locus_tag="PKO10_06420"
     CDS             complement(174..1250)
                     /gene="aroC"
                     /locus_tag="PKO10_06420"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014469065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HNW66818.1"
                     /translation="MNNFGHRFRFTTFGESHGKALGCIVDGVPAGIKIDEEFIQAEMN
                     RRKPGQNEFATKRSEGDVVEILSGVFEGYSTGTSIAMIIFNENQKSSDYSNIKDLFRP
                     GHADFTYFNKYGIRDYRGGGRSSARETAARVAAGAIAKLLLNELNIDIKSGICEIDGI
                     KADNFNFENTRNSEIFALDSNVEDAQKNAILKAKNSHNSVGGVALINVKNCPVGLGEP
                     IYHKLDAQIANAMMSINAVKAVEIGDGILASRVLGFDNNDQIRKSGFKTNHSGGILGG
                     ISNGDDINVKVYFKATPSIFIEQETVDIYGNEVECKLKGRHDPCVAIRGSVVAESMIS
                     LVIADMLLLNMSSKVENIKKVYAK"
     gene            complement(1247..>1381)
                     /locus_tag="PKO10_06425"
     CDS             complement(1247..>1381)
                     /locus_tag="PKO10_06425"
                     /inference="COORDINATES: protein motif:HMM:NF012265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative dsRNA-binding protein"
                     /protein_id="HNW66819.1"
                     /translation="HKKEFEVSIWIDGKNFGKASGKSKKLAQQAAAKIAIAKLSEEEN
                     "
BASE COUNT          434 a          264 c          148 g          535 t
ORIGIN      
        1 cttcatagct taaatcttta ttatttgtag aaaattgtag ttttgaacca tctttgaaat
       61 atataaaatt atcttcatat ttttctaaaa aaagaggata agcaagaatt aacctttttg
      121 caagattttc atttgcaaag aggtttaaag atagtaaaag tagaaataaa actctatttc
      181 gcataaactt ttttgatatt ttctactttt gatgacatat ttaataaaag catatctgca
      241 attacaagag agatcataga ttcagcaaca acacttcctc ttatagcaac acaaggatca
      301 tgtcttcctt ttagtttaca ctctacttca tttccataaa tatcaactgt ctcttgctct
      361 ataaaaatag atggagttgc tttaaagtat acttttacat ttatatcatc accatttgag
      421 attcctccca aaattccacc actatggttt gttttaaatc cagattttct tatttggtca
      481 ttgttatcaa atcccaaaac tctagatgct aaaataccat caccaatttc aacagctttt
      541 acagcattta tactcatcat tgcatttgct atttgtgcat ctaatttatg ataaattggt
      601 tcaccaagtc caactggaca attttttaca ttaataagtg caaccccacc aacactatta
      661 tgagagtttt tggcttttaa aatagcattt ttttgagcat cttcaacatt tgaatcaagt
      721 gcaaaaattt ctgaatttct agtattttca aagttaaaat tatctgcttt tataccatct
      781 atctcacaaa ttccactttt tatatcaata tttaattcat ttaatagaag ttttgcaata
      841 gctccagctg caactctagc tgctgtttca cgtgctgagc ttcttcctcc acctctataa
      901 tctctaatcc catatttatt aaaatatgta aaatcagcat gtcctggtct gaatagatct
      961 tttatatttg agtagtcact tgatttttga ttttcattaa aaataatcat agcaatgctt
     1021 gttcctgttg aataaccttc aaaaactcca cttaaaatct caactacatc accttcggat
     1081 ctttttgttg caaactcatt ttgtccaggt tttcttctat tcatttcagc ttgaataaac
     1141 tcttcatcaa tttttattcc agctggaaca ccatcaacta tacatcctag tgcttttcca
     1201 tgtgattcac caaatgttgt aaatctaaat ctatgtccaa aattattcaa ttctcttcct
     1261 cacttagttt agcaatagca atttttgcag ctgcttgttg agctaatttt ttactttttc
     1321 cactagcttt tccaaaattt ttaccatcaa tccaaataga tacttcaaac tcttttttgt
     1381 g
//
LOCUS       DAOQAN010000406         1379 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_151243_length_1379_cov_3.603474, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000406 DAOQAN010000000
VERSION     DAOQAN010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1379
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151243_length_1379_cov_3.603474"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1160)
                     /locus_tag="PKO10_06430"
     CDS             complement(<1..1160)
                     /locus_tag="PKO10_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008351251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HNW66820.1"
                     /translation="METMSDMSYIIDTLYVLFAMTLVVFMYPGFAMLEAGIVRTKNVT
                     AVLTINILIFAIASLAFVLVGYSLAFGEGLNDENINYATVLFQMAFVGKAMNVMSGGI
                     SERARVIPIAIFTVIMSSVLYPLVVNLTWGSNFLKDTVLDISSMHDLAGSTIIHSTGG
                     WALLAALLIVGARTGRYTKEGGVRVIPASNIPLVTLGALLLWIGWFGFNGGSVGSIAS
                     KEDAHLVSLVILNTNTAGFAGAIAVAIMMYIMYKKFDITMILNGGLGGLVSITAAADV
                     VGVYSPILIGAIGGILVVLGVFMFDKLRIDDPVGALSVHLINGIWGTLAVGIFASNGD
                     DITFLGQLKGVVFVGVLVFISSYVLLYILHKIMPVRAKRDQEMQGLDVEECG"
BASE COUNT          519 a          271 c          156 g          433 t
ORIGIN      
        1 aaaccgcact cttcaacatc taaaccttgc atttcttgat ctcgttttgc tcttactggc
       61 attatcttat gtaagatata caacaataca tacgaactta taaaaacaag tactccaaca
      121 aacacaaccc cttttaattg tcctaagaat gtgatatcat caccatttga agcaaaaatt
      181 cctactgcta atgttcccca aattccattt attaaatgta ctgataaagc tccaacagga
      241 tcatctattc ttaatttatc aaacataaat acaccaagaa caaccagtat tccaccaatt
      301 gctcctatta aaataggaga gtaaacacca actacatcag ctgctgctgt aattgaaaca
      361 agacctccta aaccaccatt taaaatcata gtgatatcaa attttttgta cataatatac
      421 atcataattg ccactgcaat tgcccctgca aaaccagctg tatttgtatt taaaattact
      481 aatgaaacaa gatgtgcatc ctcttttgaa gcaatacttc ctacacttcc accattaaat
      541 ccaaaccaac caatccataa aagtaaagct cccaaagtaa ccaaagggat atttgaagca
      601 ggaataactc taactccacc ctcttttgta tatcttccag ttctagctcc tactattagt
      661 aaagcagcta ataaagccca accaccagta ctgtgtataa ttgtagaacc tgctaagtcg
      721 tgcattgatg atatatctaa gactgtatct tttaggaaat ttgaacccca agttaaattt
      781 accactaaag gatataaaac agaactcata attactgtaa atattgctat tggtattact
      841 ctagctcttt cactaatacc accactcata acattcattg cctttccaac gaaagccatc
      901 tgaaataaaa ctgttgcata atttatattt tcatcgttta gtccttctcc aaatgctaaa
      961 gaataaccaa ctaaaacgaa tgctaaagat gcaattgcaa aaataagaat attgatagtt
     1021 aagacagcag taacattttt tgttctaaca attccagctt ccaacatagc aaagccagga
     1081 tacataaaaa caactaaagt cattgcaaac agtacgtaga gtgtgtcaat aatatatgac
     1141 atatcagaca tagtttccat aaagtagtcc ttttagagag taaagtctcc agcgctggac
     1201 gagcagaatt ttatcaaata aaaaattaaa taaagataaa aatatcttac ttttagctta
     1261 aaatttaaat atttatatat tttatatatt ttatgtatag tttcgtaaca tatttcaaaa
     1321 tatttttttg attaatattt aaattacttt gtttgttata taataaaaaa aatttagga
//
LOCUS       DAOQAN010000407         1378 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_151513_length_1378_cov_1.693878, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000407 DAOQAN010000000
VERSION     DAOQAN010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1378
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151513_length_1378_cov_1.693878"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            266..>1378
                     /locus_tag="PKO10_06435"
     CDS             266..>1378
                     /locus_tag="PKO10_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNW66821.1"
                     /translation="MSEKYEPSKIEDSFYKIWEDRGYFEIDGNRSIQEPNKNFAIMMP
                     PPNVTGSLHIGHALTFTLQDIITRYKRMDGYKTLWQPGTDHAGIATQNVVEKQLLKEG
                     TTKEALGREKFLERVWKWKEFSGGTIVHQMRKLGVSPAWSRERFTMDEGLKEAVKEAF
                     VKLYDEGMITQNNYMVNWCTHDGALSDIEVEHEEVNGKFYHMNYHFADGSGFVTVATT
                     RPETYFGDTAIMVHPDDERYTNIVGREVVLPLTNRKIKVITDSHVDMSFGTGVVKVTP
                     AHDTNDYEVGLRHNLEFIKCFDEKGILNDYCGEFAGLERLEARPIIVKKLQDEGFIVK
                     IEDHIHQVGHCYRCKNIVEPYISKQWFVKKEVAKKSI"
BASE COUNT          520 a          170 c          269 g          419 t
ORIGIN      
        1 ctcctttggt ttagcttctc tttttatagt tgttttttta ccattttcta aagttacttc
       61 aactccagaa ctttgtaaat cagcacttag tagagaagtg gctacaagta aacttcctaa
      121 aattatttta aacataaact ctccttgttt taaaattgta tggagtttga ttttatcaaa
      181 aacttttaaa aatgacaact atcagggact taagtgaaat ttagataaaa tctatgccat
      241 tttagtaaca caattaggat tatttatgag tgaaaagtac gaaccatcaa agatagaaga
      301 tagtttttat aaaatttggg aagatagagg ttattttgag attgatggaa acagatcaat
      361 tcaagagcca aataaaaatt ttgcaattat gatgccacct ccaaatgtaa cgggaagctt
      421 acatattgga catgcactta catttacact gcaagatatt attacaagat ataaaagaat
      481 ggatgggtat aaaactcttt ggcaaccagg aactgaccac gctggaattg caactcaaaa
      541 tgttgttgaa aaacaacttt taaaagaggg cactacaaaa gaggctttag gaagagaaaa
      601 attcttagaa agagtttgga agtggaaaga gtttagtggt ggaacaatag ttcatcaaat
      661 gagaaaacta ggagttagtc ctgcttggtc aagagagcga tttactatgg atgagggttt
      721 aaaagaggct gtaaaagagg cttttgttaa actttatgat gagggtatga ttactcaaaa
      781 caactatatg gtaaattggt gtacacatga tggagcatta agtgatattg aagttgaaca
      841 cgaagaggta aatggtaaat tctatcatat gaactatcat tttgcagatg gaagtggctt
      901 tgtaacagtt gcaacaacaa gacctgaaac atactttggt gatacagcta taatggttca
      961 tccagatgat gaaagataca caaatattgt aggacgtgag gtagttttac ctttaacaaa
     1021 tagaaaaata aaagtaatta cagattctca tgttgatatg agttttggaa caggtgtagt
     1081 taaagttact ccagctcacg atacaaatga ctatgaagta gggcttagac acaatctaga
     1141 gtttataaaa tgctttgatg aaaaaggtat tttaaatgat tattgtggag aatttgctgg
     1201 acttgaaaga ttagaagcaa gaccaataat tgttaaaaaa ctacaagatg aaggatttat
     1261 tgtaaaaata gaggaccata ttcatcaagt agggcattgc tatagatgta aaaacatagt
     1321 tgaaccatat atttcaaaac agtggtttgt aaaaaaagag gttgctaaaa aatcaatt
//
LOCUS       DAOQAN010000408         1377 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_151600_length_1377_cov_2.590772, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000408 DAOQAN010000000
VERSION     DAOQAN010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1377
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151600_length_1377_cov_2.590772"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(134..>1377)
                     /locus_tag="PKO10_06440"
     CDS             complement(134..>1377)
                     /locus_tag="PKO10_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNW66822.1"
                     /translation="ENISRTKNLIEQDRVLIDDVKRVVNEVKEGHLDRRIEKSTVNPS
                     LEELKNSFNYMIEITKQNVCKDINRLLLLLEDFEKLDFRGRISGDDGKIVVGINKLAD
                     IINHMLVENKSNGLTLEESSKMLLFNVNKLNISSNEAAASLEETAAALEEITSNIRNN
                     TESIAKMSQLSNGVTKAVNEGQAMANQTTTAMDEINTQVNLVNEAIGVIDNIAFQTNI
                     LSLNAAVEAATAGEAGKGFAVVAQEVRNLATRSAEAAKEIKAIVERATVKANEGKEIA
                     TNMIEGYKNLNSNISSTMNLISDIENASKEQLLGIEQINDAVNQLDQQTQQNAMVASQ
                     SHDIAQSTDEIAKLIVQDANQKEFEGKNEVKAKDVGIKKERKENIITSSPKKIIKPSK
                     PNINSTKEIKSNSNDDEWESF"
BASE COUNT          352 a          249 c          159 g          617 t
ORIGIN      
        1 ctaaactctt ctcttgcata tttaaccttg tttccttgtg caataaagct agttttaatg
       61 cttaaaaact agcttactta tatcttttat tttgaaatat tacgcatagc tttttactat
      121 gcgtaattaa attttaaaaa ctctcccact cgtcatcatt agaattactt tttatctctt
      181 ttgtagagtt aatatttggt tttgaaggtt tgattatttt tttaggtgat gaagttatta
      241 tattctcttt tctttctttt tttatcccta catcttttgc ttttacttca ttttttcctt
      301 caaactcttt ttggtttgca tcttgcacta ttagttttgc tatctcatct gtgctttgtg
      361 caatatcatg tgattgagat gctaccattg cattttgttg tgtttgttga tctagttgat
      421 ttactgcatc atttatctgt tcaattccta gaagttgttc tttagatgca ttttcaatat
      481 cagatattag attcattgtt gaagatatat tactatttag gtttttatac ccttcaatca
      541 tatttgtagc aatctcttta ccttcatttg cttttactgt tgctctttct actattgctt
      601 ttatctcttt tgcagcttct gcacttcttg ttgctagatt tcttacctct tgagcaacaa
      661 cagcaaatcc ttttccagct tcaccagcag ttgcagcttc aacagcagca tttaatgaaa
      721 gaatatttgt ttgaaatgca atattatcaa ttactccaat agcttcattt actaaattta
      781 cttgagtatt tatctcatcc attgcagttg ttgtttgatt agccatagct tgaccttcat
      841 ttactgcttt tgttacacca tttgaaagtt gagacatctt tgcaatactc tctgtattat
      901 ttctaatatt acttgttatc tcttctagtg ctgctgctgt ctcttcaagt gaagctgctg
      961 cttcatttga gcttatattt agtttgttta cattaaaaag aagcattttt gaactctctt
     1021 ctagtgtaag accatttgat ttgttttcaa ctaacatatg atttataata tctgctagtt
     1081 tattgatacc aacaactatt ttaccatcat caccgcttat tctacctcta aaatctaatt
     1141 tttcaaaatc ttctaataaa agtagcaatc tatttatatc tttacaaaca ttttgtttcg
     1201 ttatctctat catatagttg aagctatttt ttaactcttc caaagaagga ttaacagtgc
     1261 ttttttctat tcttctatct aaatgcccct cttttacctc atttacaact ctttttacat
     1321 catcaattaa aactctatct tgttcaatta gatttttagt tctagagata ttttcat
//
LOCUS       DAOQAN010000409         1374 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_152194_length_1374_cov_1.868082, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000409 DAOQAN010000000
VERSION     DAOQAN010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152194_length_1374_cov_1.868082"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            73..864
                     /locus_tag="PKO10_06445"
     CDS             73..864
                     /locus_tag="PKO10_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66823.1"
                     /translation="MDIGSINSTSSIQNLNNLNNNSNIALNKAQLKYSIDDFKNNSSE
                     ALAVSRSSILKSEFSQDIQSINDGIAKSQISQASMDKLQDFMKNIQNKLENSQNYENK
                     NDLKQEINQELRNFNQQAFDTKYKEENLIASRYNDEKESIQISSSNNLFSIDKPNVAN
                     FTNDIFDVVNNNDLNNPQNVENALNKVTNTSKQLQELSGQFVDFSKQLQADAKNKIIE
                     QNYNQTIDFGRESSNFSKANININAGYLAASQANVIQEQSVRLLS"
     gene            complement(872..1090)
                     /locus_tag="PKO10_06450"
     CDS             complement(872..1090)
                     /locus_tag="PKO10_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66824.1"
                     /translation="MDIKDLEDSLKLLKQKTIEELTEMVGKGRIEYLEIGTYIYRLFF
                     DALEKENSIVFDDGLREGVAINYALSKK"
     gene            complement(1131..>1374)
                     /locus_tag="PKO10_06455"
     CDS             complement(1131..>1374)
                     /locus_tag="PKO10_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66825.1"
                     /translation="CGHLPEMNSFNMYNVCKDLFAQIPTVILNSLFIDEVKKRKSNTQ
                     IIKSFPIELRYVCLSMNISQNRYRLLENKLENKIRI"
BASE COUNT          555 a          194 c          149 g          476 t
ORIGIN      
        1 taattgattc taaaatattt tattattttt ctgatataat aatatacaaa atctttttta
       61 aggagattac atatggatat aggttcaata aatagtacaa gcagtattca gaatttaaat
      121 aatttaaaca ataattctaa cattgcttta aataaagcac aactaaaata tagtattgat
      181 gattttaaaa acaattcatc agaagcttta gctgtaagta gaagttctat tttaaaaagt
      241 gaattctctc aagatattca atcaataaat gatggtattg caaaatctca aatctcacaa
      301 gcttcaatgg ataagcttca agattttatg aaaaatattc aaaataaact cgaaaatagt
      361 caaaattatg aaaataaaaa tgatttaaag caagagataa atcaagagtt aagaaacttc
      421 aatcaacaag cttttgatac aaaatacaaa gaagaaaatc ttattgcaag tagatataat
      481 gacgaaaaag agagtataca aataagctct agcaataatc tattttcaat cgataaacca
      541 aatgttgcaa attttacaaa tgatattttt gatgttgtca acaataacga cttaaacaat
      601 ccacaaaatg ttgaaaatgc tttaaataaa gtaacaaata cttcaaaaca acttcaagaa
      661 ttatctggtc aatttgtaga tttttcaaaa cagctacaag ctgatgcaaa aaataagatt
      721 atagaacaaa actataatca aactattgat tttggaagag aatcaagtaa cttctcaaaa
      781 gcaaatataa atataaatgc tggatattta gctgcttcac aagctaatgt aattcaagaa
      841 caaagtgtaa gactcctttc ttaacggagc ttcacttttt gcttaaagcg taatttatag
      901 caacaccctc tcttaatccg tcatcaaata caatagagtt ttctttttcc aaagcatcaa
      961 aaaataatct ataaatatat gttccaattt ctaaatattc aattctacct tttcctacca
     1021 tttcagttaa ctcttcaatt gttttttgtt ttaaaagttt taaactatct tctaaatctt
     1081 ttatatccaa aatagttcca tttactatat ttttatcata attaaaatag ctaaatccta
     1141 attttatttt ctaatttgtt ttctaataac ctatatctgt tctgagaaat attcattgaa
     1201 aggcatacat acctcaattc aattggaaaa cttttaataa tttgagtatt tgattttctt
     1261 ttttttacct catcaataaa aagactattc aatattactg taggaatttg agcgaataag
     1321 tctttgcaaa cattgtacat attgaaacta ttcatttctg gcaaatggcc acag
//
LOCUS       DAOQAN010000410         1371 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_152684_length_1371_cov_2.306231, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000410 DAOQAN010000000
VERSION     DAOQAN010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1371
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152684_length_1371_cov_2.306231"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="PKO10_06460"
     CDS             complement(<1..318)
                     /locus_tag="PKO10_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNW66826.1"
                     /translation="MNDISNLKNITVLYVEDEKDLREVTSSILQSFTKNQYIATNGQE
                     GYELFLKHDSDIDLIISDINMPILNGLEMIKKIKDINKNVPIIVTTAFSNKEYLLEAI
                     DIGV"
     gene            401..1321
                     /locus_tag="PKO10_06465"
     CDS             401..1321
                     /locus_tag="PKO10_06465"
                     /inference="COORDINATES: protein motif:HMM:NF023578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase
                     metal-binding domain-containing protein"
                     /protein_id="HNW66827.1"
                     /translation="MIKIECNHCHLSFDEKIMIKENDLNFCCGGCQSVYHILKSENLD
                     SFYEKLGNKTIKAPLQVSNDDLAKFDSENFLNSYTTITKDGFVQIDLILEGIHCAACV
                     WLNEKVLYDTKGVVEANINFTTNKARVVFNSDILKLSDIIKKIRSIGYNAYAYDSSIA
                     DKEASKAKQDYFVRMMVAVVCTMNIMMLSVAKYTGFFTGMSLEVKNMIHLAEFILTTP
                     VLFFSGFVFYKGAYFGLKNRIVNMDLLVSSGATMTYVYSLFVLFGAKGESYFDSVAMI
                     ITFVLVGKYLEVIGKKSAIDTLDKIKSTLP"
BASE COUNT          505 a          145 c          209 g          512 t
ORIGIN      
        1 aacaccaata tcaatcgctt ctagtagata ctctttgttt gaaaaagcag ttgttactat
       61 aattggaaca tttttattta tatctttaat ttttttaatc atctctaaac catttaaaat
      121 tggcatattt atatcagaga taattaaatc aatatcgcta tcatgtttta aaaagagttc
      181 atacccttct tgaccatttg ttgcaatata ttgatttttt gtaaatgatt gtaaaataga
      241 agatgtaact tctcttaaat ctttttcatc ttcaacatac agcactgtaa tatttttaag
      301 atttgatata tcgttcatta aaccactctt tgaaattaat aagatataat acacaaaaat
      361 cttaatataa agtttaattt taaaaaaagg ggatgttttt ttgataaaaa tagagtgtaa
      421 tcattgtcat ttatcatttg atgaaaaaat aatgataaaa gagaatgatt taaatttttg
      481 ttgcggtggt tgccaaagtg tttatcatat tttaaaaagt gaaaatttag actcttttta
      541 tgaaaaactt ggaaataaaa ctataaaagc tcctttacaa gtatcaaatg atgatttagc
      601 aaaatttgac tcagaaaact ttttaaatag ttacacaaca atcacaaaag atggctttgt
      661 acaaattgat ttaatacttg aaggaattca ttgtgcagct tgtgtttggc taaatgaaaa
      721 ggttttgtat gatacaaaag gtgtagttga agcaaatata aatttcacaa caaacaaagc
      781 aagagttgtt tttaatagtg atattttaaa acttagtgat attataaaaa agataagaag
      841 tattggttac aatgcttatg cttatgattc aagtatagca gacaaagaag ctagcaaagc
      901 aaaacaagac tattttgtaa gaatgatggt tgcagttgtt tgtactatga atattatgat
      961 gttaagtgtt gcaaaatata caggcttttt tacaggaatg agtcttgaag taaaaaatat
     1021 gatacatcta gcagagttta ttctaacaac tccagttcta ttttttagtg gatttgtatt
     1081 ttataaaggt gcatattttg gtttaaaaaa tcgtattgta aatatggatt tgcttgtaag
     1141 ttctggtgca acaatgactt atgtttactc tttatttgtt ttatttggtg caaaaggtga
     1201 gagctatttt gattctgttg ctatgattat aacttttgtt ttagttggaa aatatcttga
     1261 agtaattggt aaaaaatcag ctattgatac tttagataaa ataaaatcta ctcttcctta
     1321 gaagctattg ttgtaaaaga tgggaaaaaa gagacaaaag ctttaaattt a
//
LOCUS       DAOQAN010000411         1370 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_152789_length_1370_cov_3.447148, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000411 DAOQAN010000000
VERSION     DAOQAN010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152789_length_1370_cov_3.447148"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1286)
                     /locus_tag="PKO10_06470"
     CDS             complement(<1..1286)
                     /locus_tag="PKO10_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66828.1"
                     /translation="MGKTHSMSNKIFEDVDATMEESQIRRQELIDQYNMAEDNPNAKH
                     KVYQLRLKEGFDQNGNPIYSYKTGIAETSAAERYKNQYIKDGYEILSEKGFAGAEDWE
                     NRWHGLKANLADRTYDEGINSQGQKIKDLSSIGDGYSEVYNTQAFDFGKTDQEIAQNM
                     ANSQNLSDIRAERLAEGYGRGSDSIIDSIQAGGLKTLADTGDIILDFVTPGDNTLLND
                     AKDQTKIDKYVGYNRKTADRAIGEATEYFKNGNYAKAAWEIYKEPQIIAESIPAMAEM
                     LLGFGKFTKAGKLAMGVDAANKAGDVAAASKLTKELAENVSNSQKAMYRVAQNAGLLT
                     FAASQTNNQIEERVKNNQQAGLTGGDSIGEVATVFASNLALYGLDRGLFTKITGIGGG
                     KAALSDAFGFANIQTKKNIMRTVAEKMLAAGTAGAA"
BASE COUNT          341 a          290 c          203 g          536 t
ORIGIN      
        1 tctgctgcac cagctgtacc agctgcaagc attttttccg ctacagttct cattatattt
       61 tttttagttt gaatattagc aaatccaaac gcatcagaca gagcggcttt tcctccacca
      121 ataccagtga ttttagtgaa aagtcctctg tctaatccat atagagccag attcgatgca
      181 aatacagtag caacttctcc aatgctatct ccgccagtaa gtccagcttg ttgattgttt
      241 ttaactcttt cttctatttg attatttgtt tgactagcag caaaagttaa aagaccagca
      301 ttttgtgcaa ctctgtacat tgctttctga gaattgctaa cattctctgc taattctttt
      361 gttaattttg aagcagcagc aacatcacct gctttatttg cagcatctac gcccatagct
      421 agtttaccag cttttgtgaa ctttccaaaa cctaaaagca tttctgccat agctggaata
      481 ctctctgcta tgatttgagg ttctttgtaa atttcccaag cagctttagc atagtttcca
      541 ttcttgaaat actcagtagc ttcacctatg gctctatctg cagtttttct gttatatcca
      601 acatatttat ctatctttgt ttgatctttt gcatcattca ataaagtatt gtcacctggc
      661 gtaacaaaat caagaataat atctccagta tctgctagag tttttaatcc tcctgcttga
      721 atagaatcta taatactgtc agaacctctt ccatatcctt ctgctaatct ttctgctcta
      781 atgtctgaaa ggttttgact attagccata ttttgagcaa tttcttggtc tgtttttcca
      841 aagtcaaatg cctgagtatt gtatacttct gagtatccat ctccgatgct acttaagtct
      901 tttatttttt gaccttgaga gtttattcct tcgtcgtaag ttctgtcagc aagatttgct
      961 ttcaatccat gccatctgtt ttcccagtcc tctgcaccag caaaaccttt ttcgcttaat
     1021 atttcatatc catcttttat gtattggttt ttgtatcttt ctgcagcact tgtttctgct
     1081 ataccagttt tatacgagta gattggattt ccattttggt caaatccttc tttcaatcta
     1141 agttgataaa ccttgtgctt tgcatttgga ttgtcttctg ccatgttgta ttggtctatt
     1201 aattcttgtc ttcttatttg actttcttcc attgtagcat caacatcttc aaaaatctta
     1261 ttgctcatag aatgagtttt acccatatag ttagctttta aatcttctcc atcccatagg
     1321 tcattgacag tattgtaaag agattttgct ttaagggtga agttgctatc
//
LOCUS       DAOQAN010000412         1368 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_153182_length_1368_cov_3.226961, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000412 DAOQAN010000000
VERSION     DAOQAN010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153182_length_1368_cov_3.226961"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="PKO10_06475"
     CDS             complement(<1..253)
                     /locus_tag="PKO10_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66829.1"
                     /translation="MKSILLSIVAIALFTACSGKKYYEPEETSSNIELNKKSMSSSIK
                     SMNRVGATLEDNEVITKQGISSFKLPEGFEFLNFTDDGKV"
     gene            complement(264..842)
                     /locus_tag="PKO10_06480"
     CDS             complement(264..842)
                     /locus_tag="PKO10_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66830.1"
                     /translation="MSIKDDVNYIKNELSSEEKFLESFVKTERFFKKYKKLIVVLIIT
                     VIVGSIAFLVKTKLDEKNLYEANIALSSFLENGNQNSLDELKEKNRDLYEIALYLDAK
                     NEFKNADINLKYLKELLDFQVALLNSNQSDLDAVSKKADFLLKDYAIFNQALILVNNQ
                     KYAEAREILGKISQDSRAFELATLLKHYLVTK"
     gene            956..>1368
                     /locus_tag="PKO10_06485"
     CDS             956..>1368
                     /locus_tag="PKO10_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014469387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNW66831.1"
                     /translation="MNKLLIATSIAFVLSQNIFAKEAPVENEQTRFESLSKLTKVIGT
                     VEKYYVDDIKLQEIVDKALKGLMQELDAHSSYLDKKASKEMSIATSGEFGGLGITVGM
                     RDGALTVISPIDDTPAFKAGVKSGDIILKINETST"
BASE COUNT          481 a          193 c          170 g          524 t
ORIGIN      
        1 taacttttcc atcatccgta aaatttaaaa actcaaatcc ttctggaagt ttaaagctag
       61 aaattccttg tttcgtaata acttcattat cttctaaagt tgcacctact ctattcatag
      121 attttattga agatgacata gactttttgt ttagctcaat attactactt gtctcttctg
      181 gttcataata ttttttacca gaacaagctg taaaaagtgc aattgcaaca attgacaata
      241 gtatagattt cattttttat tccttatttt gtaactaaat agtgttttaa aagtgttgct
      301 agttcaaaag ctcttgaatc ttgagatatt ttacccaaaa tttctcttgc ttcagcatat
      361 ttttgattat taactagtat taaagcttga ttaaaaatag cataatcttt aagtaaaaaa
      421 tctgcttttt ttgaaactgc atccaaatct gattgattag aatttaaaag tgcaacttgg
      481 aaatctagta actcttttaa atatttaaga ttaatatctg catttttaaa ctcattttta
      541 gcatctaaat acaaagctat ctcgtataaa tctctatttt tctcttttag ttcatctaaa
      601 gagttttgat ttccattttc aaggaaacta cttagagcaa tattcgcttc ataaagattt
      661 ttttcatcca atttagtttt tactaaaaat gctattgaac caactattac agtaataatt
      721 aatacaacta ttaatttttt atatttttta aaaaatctct cagtttttac aaaactttca
      781 aggaatttct cttcactact taactcattt ttgatgtagt ttacatcatc ttttatactc
      841 atatacctag cctttttaaa aagtagttga tatattacaa aatttttcat aaaatattac
      901 ttttataaac ttttttgtat aattttagcc tataaattta aaaggttatc gcaaaatgaa
      961 taaactatta atagcaacat ctattgcatt tgtattatct caaaatatat ttgcaaaaga
     1021 agcgcctgtt gaaaacgaac aaacaagatt tgaatcttta tcgaaactaa caaaagttat
     1081 tggaactgtt gagaagtatt atgttgatga tattaaactt caagagattg ttgacaaagc
     1141 actaaaaggt ttaatgcaag aattagatgc ccattcaagc tatttggaca aaaaagctag
     1201 taaagagatg agtattgcaa catctggaga atttggtggg cttggtatta ctgttggtat
     1261 gagagatggt gcattaacag taatatcacc aatagatgat actcctgcat ttaaagctgg
     1321 tgtaaaatct ggtgatataa ttttaaaaat aaatgagact tcaactat
//
LOCUS       DAOQAN010000413         1367 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_153433_length_1367_cov_2.086128, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000413 DAOQAN010000000
VERSION     DAOQAN010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1367
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153433_length_1367_cov_2.086128"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(143..>585)
                     /locus_tag="PKO10_06490"
     CDS             complement(143..>585)
                     /locus_tag="PKO10_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014473635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNW66832.1"
                     /translation="TIKDEIILPALLIDIGKYIIADMILNEQKEKEFKAQIEEGILSI
                     EEVEKEFLGFSSSYVTAQIFKHWKLSPNLINSIEFVDDINSVSKEFERKAKILSVIKT
                     VANIKEPLSEKSIKEGIIKAKEYGFDTKILAQTIENLKTKIDLY"
     assembly_gap    586..595
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<596..916)
                     /locus_tag="PKO10_06495"
     CDS             complement(<596..916)
                     /locus_tag="PKO10_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014473635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNW66833.1"
                     /translation="MQIDDLLRRVESLPPLPRTIILIEEYRRKSEKEISELHDIISKD
                     ALIVTNLLKIANSAMFGFRSKVETPLRAISLLGINFTISVAISASSQKLLVTLQVQFI
                     VEIIF"
     gene            complement(937..>1367)
                     /locus_tag="PKO10_06500"
     CDS             complement(937..>1367)
                     /locus_tag="PKO10_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HNW66834.1"
                     /translation="VKEHASNVGADVIILCAKIEEELVGLEDDEARAFLDDLGVAESG
                     LEQIIQKAFDKLGLQSYFTAGKIEVRAWTIRKNTKAPQAAAVIHNDFEKGFIKAEVIS
                     YEDFVKFGGEAKCKDAGKLRLEGKDYIVQDGDIMHFRFNV"
BASE COUNT          446 a          235 c          141 g          535 t
ORIGIN      
        1 gttcacaaaa gttgcataag aggaaaagtt tttgattatt taaaaataaa agagcttttt
       61 tttacaaaaa aaactataaa tctaaatgat gatgaagtag aaaatctttt tgaaatccta
      121 cttcttgggc tttgaaatag agttaatata aatctatttt tgttttaaga ttttctatgg
      181 tttgagctaa tatttttgta tcaaatccat actcttttgc ttttataatt ccttctttaa
      241 ttgatttttc gcttaaaggt tcttttatat ttgcaacagt ttttattaca cttaaaattt
      301 ttgcttttct ttcaaactct tttgaaacac tatttatatc atctacaaat tctatactat
      361 ttatcaaatt tggactaagt ttccagtgtt taaaaatttg tgctgttaca tatgaactag
      421 aaaatcctaa aaactctttt tctacctctt caatagataa aattccttct tctatttgtg
      481 ctttaaactc tttttctttt tgctcattta gaatcatatc agcaataata tatttaccaa
      541 tatcaatcaa aagtgcaggt aaaataatct catcttttat agtttnnnnn nnnnnaaata
      601 tgatttccac tataaattgc acctgaagtg taactaatag tttttgacta ctagctgaaa
      661 tagcaaccga tattgtaaag tttattccaa gtaaagatat cgctctaagt ggagtttcaa
      721 ctttacttcg aaaaccaaac atagctgaat ttgctatttt taaaagattt gttacaatca
      781 aagcatcttt acttataata tcatgaagtt cacttatctc tttttcactt tttcttctat
      841 actcttctat taaaataata gttctaggaa gaggtggaag ggattcaact cttcttaata
      901 aatcatcaat ttgcaaaata tccccttgaa ttttatctaa acattaaatc taaaatgcat
      961 tatatcacca tcttgaacaa tataatcctt accttctagt cttaattttc cagcatcttt
     1021 gcatttagct tctccaccaa atttaacaaa atcttcataa gatataactt ctgcttttat
     1081 aaaacctttt tcaaagtcat tgtgaataac agcagctgct tgtggagctt ttgtattttt
     1141 tctaattgtc caagcacgaa cttctatttt tccagctgta aaatagcttt gaagacctaa
     1201 tttatcaaaa gctttttgaa taatttgctc taaacctgat tcagcaactc ctaaatcatc
     1261 caaaaatgct cttgcttcat catcttctaa acctacaagc tcctcttcaa tttttgcaca
     1321 taaaataata acatccgcac ccacatttga tgcatgctct tttacca
//
LOCUS       DAOQAN010000414         1364 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_154002_length_1364_cov_1.945760, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000414 DAOQAN010000000
VERSION     DAOQAN010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_154002_length_1364_cov_1.945760"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1364
                     /locus_tag="PKO10_06505"
     CDS             <1..>1364
                     /locus_tag="PKO10_06505"
                     /inference="COORDINATES: protein motif:HMM:NF014075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HNW66835.1"
                     /translation="NRKPKKKNEELIKKATFDTKNSIFQMEPYFTIDDKIEVDFKKRF
                     ELEKLNDLNNLTLRDVQEYTNFTKDYLKTKDKKKFKKIDKFIFLGTLLGRHIPKIAEK
                     IDASSYLVLERNLEIFRLSLFTVDYTILAKKGAIFSIMDNIEEEKKIYTFLDVYKIDN
                     YFLKLSITNINISQYIDTILNLLLTLNPTAYDYNRRLYIHTNRTTRRIKEGYKFLDFK
                     KIKDNSNFFENRPILYIAAGPSLDDNLEWVKKNQNNFFIVCIGRALKKLVDNEIRVDV
                     VATLDEQEFLADTQFDDETIRKLGKNTLLFAGSITNENVLNKFDKKNIFIFEVFYCLY
                     KGNSAYGGFSIGEIALHLLLEFKPKEIFILGLDLALNQKTGATHSTGNTFGTSQINLD
                     EEQDRSNFDIRSSLVKVKGNFIKEVYTTPIFYGSIKMLEDIVRGKDKSIKIYNLSKNG
                     ARFGG"
BASE COUNT          592 a          126 c          205 g          441 t
ORIGIN      
        1 aatagaaaac caaaaaagaa gaatgaagaa ttaataaaaa aagcaacatt tgatacaaaa
       61 aactctattt ttcaaatgga accctacttt accatagatg ataaaataga agtagatttt
      121 aaaaagagat ttgaactaga aaaattaaat gatcttaata atttaacatt aagagatgtt
      181 caagaatata caaattttac aaaagattat ctaaaaacaa aagataaaaa aaagtttaaa
      241 aaaatagata aatttatatt tttaggtaca cttttaggga gacatatacc taaaattgca
      301 gagaaaatag atgcaagttc atatttagtt ttagaaagaa atttagaaat atttagattg
      361 tctcttttta cagtagatta tacgatatta gcaaaaaaag gtgccatctt ttctataatg
      421 gataatatcg aagaagaaaa gaaaatatat acatttttgg atgtatataa aatagataac
      481 tactttttaa aattatctat aacaaatata aatattagtc aatatattga tactatacta
      541 aatctacttt tgactctaaa tccaacagca tatgattata atagaagact atatattcat
      601 acaaacagga ctacaagaag aattaaagaa ggatataaat ttttagattt taagaagata
      661 aaagataatt caaatttctt tgaaaataga ccaatacttt atatagctgc aggaccatct
      721 ttggatgata atttagaatg ggtaaagaaa aaccagaata atttttttat agtttgtata
      781 ggaagagctt taaaaaaact tgtagataat gaaataagag ttgatgtagt agctactctt
      841 gatgagcaag agtttttagc ggatacgcag tttgatgatg agacaatccg taaattaggc
      901 aaaaatactc tgctatttgc tggttctata acaaatgaaa atgttttaaa taaatttgat
      961 aaaaaaaata tatttatttt tgaagttttt tattgtttat acaaaggcaa tagtgcttat
     1021 ggtggattta gtataggaga aattgcttta catttacttc ttgaatttaa accaaaagag
     1081 atatttatct taggtttaga tttagcatta aatcaaaaaa caggtgctac tcactctact
     1141 ggaaatacat ttggaactag tcaaataaat ttagatgaag agcaagatag aagtaatttt
     1201 gatataagaa gtagtttagt gaaagtaaaa ggtaatttta taaaagaggt atatacgaca
     1261 cctatatttt atggctcaat aaaaatgcta gaagatatag ttagaggaaa agataagagt
     1321 attaaaatct ataatttatc taaaaatggt gcaagatttg gagg
//
LOCUS       DAOQAN010000415         1359 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_154897_length_1359_cov_2.212423, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000415 DAOQAN010000000
VERSION     DAOQAN010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1359
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_154897_length_1359_cov_2.212423"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="PKO10_06510"
     CDS             complement(<1..197)
                     /locus_tag="PKO10_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNW66836.1"
                     /translation="MEVLEKVKNQIRDFVEVCHYPKALDLLEKLATGKMLRSKLILKI
                     AKESDESDEIIRLCAVVEMIHA"
     gene            complement(198..500)
                     /locus_tag="PKO10_06515"
     CDS             complement(198..500)
                     /locus_tag="PKO10_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2018 family protein"
                     /protein_id="HNW66837.1"
                     /translation="MSKYKDWFTEDDDDIFFGSPKSKFFDILEQTHRDLVEDEIDKVI
                     EKLAILELIISQDKDEDFDINSYLEEFKKMNLEDVNSMKKGLYMEFSGEIISRLDS"
     gene            complement(493..>1359)
                     /gene="hisD"
                     /locus_tag="PKO10_06520"
     CDS             complement(493..>1359)
                     /gene="hisD"
                     /locus_tag="PKO10_06520"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HNW66838.1"
                     /translation="AIPAKVAGVKEIVVCTPTINNEVNELLLAACHLCGVSKVYKVGG
                     ASAIAAMAYGTQTIPKVDVITGPGNIFVATAKKMVFGEVNIDMIAGPSEIGILADDSA
                     KANYIAIDLLSQAEHDEMASSILITTCENLAKNTSKEVDNFLANLSRYEIAKKSIDER
                     GVIIVASNMEEAIELMNEIAPEHLEVMTKNPFELLPFIKHAGAIFLGENTPEPIGDYI
                     AGPNHTLPTGGTAKFYSPLNVENFLKKSSIISFSKKAINDLGESCALLADTEGLTAHA
                     KSVRVRLEEKGE"
BASE COUNT          418 a          294 c          149 g          498 t
ORIGIN      
        1 gcgtgaatca tctcaacaac agcacaaagc cttataatct catcactctc atcactctct
       61 tttgcaattt taagtatcaa tttacttctt agcatctttc cagttgccaa tttttcaagc
      121 aaatccaaag cctttggata atggcaaacc tctacaaaat ctctaatttg attttttact
      181 ttttctaaaa cttccactta actatccaac ctactaataa tctctccaga aaattccata
      241 taaagccctt ttttcataga gtttacatct tctagattca tctttttaaa ctcttctaaa
      301 tatgagttta tatcaaaatc ctcatcttta tcttgactta taataagctc caaaattgct
      361 aatttttcaa taactttatc aatctcatct tcaactaaat ctctatgagt ctgctctaaa
      421 atatcaaaaa actttgactt tggacttcca aaaaatatat catcgtcatc ttctgtaaac
      481 caatctttat atttactcac ctttttcttc caatcgcact cttacgcttt ttgcatgtgc
      541 tgttaatcct tcagtatctg ctagaagtgc acaagactct cccaaatcat ttatagcttt
      601 ttttgaaaaa ctaattattg agcttttttt aagaaaattc tctacattta aaggactata
      661 aaatttagca gttccacctg ttggtaaagt atgatttggt cctgctatat aatcacctat
      721 tggctcagga gtattctcac ctaaaaatat agctccagca tgttttataa aaggaagtag
      781 ttcaaatgga ttttttgtca taacttcaag atgctcaggt gctatttcat tcataagttc
      841 aattgcttct tccatatttg aagcaacaat tataacaccc ctctcatcta ttgatttttt
      901 agcaatctca tatctgctta aatttgctaa aaaattatca acctctttgc ttgtattttt
      961 cgccaaattt tcacaagtag ttatcaaaat tgaactagcc atctcatcat gttcagcttg
     1021 agagagtaaa tcaattgcta tataatttgc ctttgcacta tcatctgcta aaatccctat
     1081 ttcactgggt cccgcaatca tatcaatatt tacttcacca aaaaccattt ttttagcagt
     1141 tgctacaaag atatttccag gtcccgttat cacatctact ttaggaattg tttgtgttcc
     1201 atatgccata gcagcaattg cacttgctcc tccaacttta taaacctttg aaacaccaca
     1261 aaggtgacaa gcggcaagaa gtagttcatt tacctcatta ttaatagttg gtgtacaaac
     1321 aactatctct ttaactcctg caacctttgc tggaattgc
//
LOCUS       DAOQAN010000416         1359 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_154899_length_1359_cov_2.197853, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000416 DAOQAN010000000
VERSION     DAOQAN010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1359
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_154899_length_1359_cov_2.197853"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            126..845
                     /locus_tag="PKO10_06525"
     CDS             126..845
                     /locus_tag="PKO10_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNW66839.1"
                     /translation="MAIKNVIVDGKNFDLSYELINPTKKEDILFLHGWGSNKDIMKSS
                     FSSYLQDYRHIYLDMSGFGKSSNNYVLTTNDYSKITKEFLKSINSNCEVIFGHSFGGK
                     VATLLNPKNLVLLSSAGILEEKPIKVKIKIFFAKLLNKLGLKNFTKVFRSKDVDKMSE
                     NMYSTFKNVVDEDFSSYFSNFQNNAFIFWGEDDSATSLKSGEKIASLIKKSIFTSYSG
                     DHYFFLKHAKNICERVENGIS"
     gene            832..>1359
                     /locus_tag="PKO10_06530"
     CDS             832..>1359
                     /locus_tag="PKO10_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HNW66840.1"
                     /translation="MEYLSIVTKIVLVLSLGWYLITNLQWYNYKLSRVIFKHHKWQWH
                     LTFFISPIVLFFIIPSPYFDIYFFVLYFVSFILWNRKLDRSLVLTSRVKRFLTILLIT
                     IFVQISLCLDDDFCSKVTLFLTLIVTLILSNIIEKILFLLFKSRAKKRLLENKDLKIV
                     AITASYGKTSIKNFLH"
BASE COUNT          512 a          137 c          184 g          526 t
ORIGIN      
        1 aactatctat tttctgattt ccaagattta tttacaaaag ttgcttcaaa tcttccaact
       61 aaaaaagata tacctataaa ttttgagtat gtaaataaaa tccaatttgc aaaaggaaaa
      121 tagatttggc tattaaaaat gtcatagttg atggtaaaaa ttttgattta tcttatgagc
      181 taattaatcc tacaaaaaaa gaagatattt tgtttttaca tggttggggt tcaaataaag
      241 atattatgaa aagttcattt tcttcttatt tgcaagatta tagacatatt tatcttgata
      301 tgtcaggttt tggtaaaagt tctaacaact acgttttgac tacaaatgat tactcaaaga
      361 ttacaaaaga gtttttaaaa tctataaatt caaattgtga agttattttt ggtcactctt
      421 ttggtgggaa agtagctacc ttattaaatc caaaaaattt agttttgcta agtagtgcag
      481 gaattttgga agagaagcca ataaaggtaa aaataaaaat attttttgca aaacttttaa
      541 ataaattagg tttaaaaaat tttacaaaag tttttagaag taaagatgtt gataaaatga
      601 gtgaaaatat gtattcaact tttaaaaatg tagtagatga agatttctct tcatattttt
      661 caaattttca aaataatgct tttatttttt ggggagaaga tgatagtgct acatctttaa
      721 aatctggaga aaagatagca agtttaatca aaaaatcaat ttttacttca tatagtggtg
      781 accactattt tttcttaaaa cacgcaaaaa atatatgtga aagagtagaa aatggaatat
      841 cttagtatag ttaccaaaat tgttttagtt ttaagtttgg gttggtattt gattactaac
      901 ttacagtggt ataactataa actaagtaga gttatattta aacaccataa atggcagtgg
      961 catttaacat tttttatctc tccaatagtt ctatttttta taattcctag cccatatttt
     1021 gatatatact tttttgtttt atattttgta agttttattt tatggaatag aaaacttgat
     1081 agaagtttag tgcttacaag cagagtaaaa agatttttga ctattttact aataactatt
     1141 tttgttcaaa tttcattatg tttagatgat gatttttgtt caaaagtaac tcttttttta
     1201 actcttattg tgactttaat tctttcaaat attatagaaa aaatactttt tttactattt
     1261 aaaagtagag caaaaaaaag attattagaa aataaagatt taaaaattgt tgcaattaca
     1321 gcatcttatg gaaaaacatc tataaaaaac tttttacac
//
LOCUS       DAOQAN010000417         1357 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_155233_length_1357_cov_2.211214, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000417 DAOQAN010000000
VERSION     DAOQAN010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_155233_length_1357_cov_2.211214"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(208..>1357)
                     /locus_tag="PKO10_06535"
     CDS             complement(208..>1357)
                     /locus_tag="PKO10_06535"
                     /inference="COORDINATES: protein motif:HMM:NF016158.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cryptochrome/photolyase family protein"
                     /protein_id="HNW66841.1"
                     /translation="YELFDNYLEKKVYKNFSNITTIKNPNFINPKDKNKFLHKFYINR
                     RKELNIFMQNGKPLFDKYSFDEDNRKKLPKDIKIPPTLAFENEFVKEAKDYCKKFKSV
                     GVCEYFYYPTTFEEASLQLNYFLKEKFENFGFYQDAITKDTKQSFLFHSNISSSLNIG
                     LIDLNELIEKIVNSKAPYNAKEGFIRQIIGWREFMLRVYEDDGILLRNSNFFEFKNPI
                     PKKIIEAKSGIQILDDTIKKLELTAYNHHIERLMILGNIFLLLEIKPNEVYEFFMKNY
                     IDAYDWVMVGNVYGMSGFSDGGSITTKPYISSSNYLLKMSDYSKNESWCEILDALYWR
                     FLYKYSFKFDKNPRMKMQIALLNKMPKYKLERHLFVAEKYLSELFIAN"
BASE COUNT          447 a          211 c          153 g          546 t
ORIGIN      
        1 acacatcttc catctggtgt gaaagtgtgg caaattccac ttgtgtgagt tgctccaagt
       61 tccccttttt tgtatgaagt ttcaactccg cttgaagagc aagatacatc atattttgca
      121 ctatttgcta aaatttccat tttttcataa atatcatttt tcattttgta attttacttt
      181 aaataaattt ttttattcaa aaatatttca atttgcaata aaaagctctg ataaatattt
      241 ctctgcaaca aaaagatgtc tttcaagttt atattttggc attttattta aaagtgctat
      301 ttgcatcttc attcgtggat ttttgtcaaa cttaaaagag tatttatata aaaatctcca
      361 gtaaagtgca tctaaaattt cacaccaact ctcattttta ctataatcac tcatttttaa
      421 taaatagttt gaacttgata tatatggttt tgtagttata cttccaccat cactaaaacc
      481 actcattcca taaacatttc caaccataac ccaatcatag gcatcaatgt agtttttcat
      541 aaaaaactcg taaacctcat ttggtttgat ttcaagtagt aaaaaaatat ttcctaaaat
      601 cataagcctt tctatatggt gattataagc tgtgagttct agttttttga tggtatcatc
      661 aagaatttgt attccacttt ttgcttcaat tattttttta ggaattggat ttttaaactc
      721 aaaaaagttt gagtttcgca agagtattcc atcatcttca taaactcgaa gcataaattc
      781 acgccaacca ataatttgtc ttataaaacc ctcttttgca ttatagggtg ctttagaatt
      841 tactattttt tctatcaact catttaaatc aatcaaacca atatttaaac tacttgagat
      901 atttgagtga aataaaaaag attgctttgt atcttttgtg attgcatctt gatagaagcc
      961 aaaattctca aacttctctt ttaaaaaata gtttagttgc aaagaggctt cttcaaaagt
     1021 tgtaggatag taaaaatatt cacaaactcc aacactctta aactttttgc aataatcttt
     1081 tgcctctttt acaaactcat tttcaaaagc caaagtaggt ggaattttta tatcttttgg
     1141 aagttttttt cgattatcct catcaaaact atatttatca aaaagaggtt ttccattttg
     1201 cataaatata tttaactctt ttcttctatt tatataaaac ttatgtaaaa acttgttttt
     1261 atcttttggg tttataaagt ttggattttt tattgtagtg atatttgaaa agtttttata
     1321 aacttttttt tccaaataat tatcaaaaag ttcatac
//
LOCUS       DAOQAN010000418         1357 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_155248_length_1357_cov_2.062212, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000418 DAOQAN010000000
VERSION     DAOQAN010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_155248_length_1357_cov_2.062212"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1033)
                     /locus_tag="PKO10_06540"
     CDS             complement(<1..1033)
                     /locus_tag="PKO10_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014469161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNW66842.1"
                     /translation="MKPAYFLMLLAFVLFYFLIELFDPFLKSISVAALLTIATNSMFL
                     RINNKVKNRAFSTTIFTLAMTALFFLPILYCIISFATFFNQVDQQHLIQNLTEIKTMI
                     IGFLGEFSFLNDFINKISSSVDIGKTVQQLVSFSASLGKNSAKFMIDMILILIFFFFF
                     TLFSNQIATYLKNITPINNEDANILFNESSSVMSVVFYSILVTAIFQGFLFGAFVSSF
                     GYDGLLLGVLYGFASLVPVIGGVIMWLPVALYEASTGTISNAIFIAVYSIIVISIIAD
                     TFIKPLIISYINKKIVKTPTKINSLIIFFAIVAGLGTFGFWGMIIGPAMVSLFVSTMQ
                     LLRKYIKEVE"
     gene            complement(1030..>1357)
                     /locus_tag="PKO10_06545"
     CDS             complement(1030..>1357)
                     /locus_tag="PKO10_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Holliday junction DNA helicase RuvB C-terminal
                     domain-containing protein"
                     /protein_id="HNW66843.1"
                     /translation="NEKNIHLKRCKYALDELGVNESGFDEMDINLLELLISNRGKPMG
                     LSTMAAALSEDEGTIEDAIEPYLLANGYIERTARGRVASVKTYEMFRLTPPQNKLDEN
                     NQGSLF"
BASE COUNT          500 a          206 c          174 g          477 t
ORIGIN      
        1 tttcaacctc ttttatatat tttcttaaaa gttgcattgt agatacaaat aagctaacca
       61 ttgcaggtcc aattatcatt ccccaaaatc caaaagtacc aagtcctgca actattgcaa
      121 aaaagattat taatgagttt atttttgtag gtgtttttac tattttttta tttatgtagc
      181 ttattattag cggttttata aaagtatctg caattattga aataactatt atagagtaaa
      241 ctgctataaa aattgcattt gatatagttc cagtacttgc ttcatataaa gctaccggaa
      301 gccacataat aactccacca ataacaggaa ctaaagatgc aaaaccatat aaaactccaa
      361 gtaaaagccc atcataacca aaactactca caaaagctcc aaaaagaaaa ccttgaaata
      421 ttgcagttac taaaatagag taaaaaacta cactcattac acttgatgat tcattaaata
      481 atatatttgc atcttcgtta ttgataggag ttatattttt tagataagtt gcaatttggt
      541 ttgaaaatag ggtaaaaaag aagaaaaaga ttaaaatcaa aatcatatca atcataaatt
      601 ttgcactatt tttacctaaa gatgcactaa aagatacaag ctgttgtact gtttttccta
      661 tatccacaga tgatgatatt ttatttataa aatcatttaa gaagctaaat tcacctaaaa
      721 agccaataat catagttttt atctctgtta aattttgtat taggtgttgt tggtccactt
      781 gattaaaaaa tgttgcaaat gagattatac aatataaaat aggtaagaaa aataatgctg
      841 tcatagcaag agtaaaaatt gttgtagaaa aggctctatt ttttacttta ttatttattc
      901 ttaaaaacat tgagtttgta gcaatagtaa gcaaagctgc tactgaaatt gattttaaaa
      961 atgggtcaaa aagttctatt agaaaataga atagaacaaa tgctaaaagc attaaaaaat
     1021 atgctggttt caaaataggc ttccttgatt attctcatct aatttatttt gaggaggagt
     1081 taatctaaac atctcatatg ttttaacact agcaactctt ccacgtgcag ttctttctat
     1141 atatccatta gcaagtaaat atggctcaat agcatcttca atagttcctt catcttcact
     1201 taatgctgct gccattgtag aaagtcccat aggttttcct ctatttgata ttagtagttc
     1261 taaaagattt atgtccattt catcaaagcc actctcattt acacctaatt catctaaagc
     1321 atacttgcat ctttttagat gaatgttttt ttcattt
//
LOCUS       DAOQAN010000419         1355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_155596_length_1355_cov_2.103846, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000419 DAOQAN010000000
VERSION     DAOQAN010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_155596_length_1355_cov_2.103846"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..532)
                     /locus_tag="PKO10_06550"
     CDS             complement(50..532)
                     /locus_tag="PKO10_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding protein"
                     /protein_id="HNW66844.1"
                     /translation="MNKKYIDEFYSRVAKDKKHEVFALSLPMFLANKHIYNESESFYK
                     TSYDLLHSDIDVLAALYFSNENHSLSPTELYDATIFSSGGMTKVLKKLEDREFIKRDV
                     SKEDKRKTFVTLTKKGVVLIESCICEASKRLENHFKVLSQKEKNDLKKILSKLIYSMI
                     "
     gene            634..>1355
                     /locus_tag="PKO10_06555"
     CDS             634..>1355
                     /locus_tag="PKO10_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014473768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNW66845.1"
                     /translation="MKKIYFTFLVPIFLYSQNLEELVNLTIENRLVESSKQNLDALKD
                     EYKSVQRGYLPKLDAGASYSINEHEYPNNPKKRANAYGSLNYLLYDGGKKYDIYDGYE
                     TNIKSGEKSLDALKNNLSLTVIQYYFDYLSLEAKKDAKQKEIEQLTAQEDRIGRFYNA
                     GTTTEDELQKIVSRLQNAIVELQEIELNIITITHNLEYITGTQVSITDGSKLQDINNL
                     IQKNPRFDIQALDLSTQSKQSIA"
BASE COUNT          539 a          179 c          165 g          472 t
ORIGIN      
        1 tggctttgaa gatgatcatg ctcttccttt atctttaatt tttatttatt taaatcatag
       61 agtaaattag ttttgataaa atttttttca aatcattttt ctctttttga cttaatactt
      121 taaaatgatt ttctaatctc tttgaagctt cacaaataca agattctatt agaacgacac
      181 ctttttttgt aagcgttaca aaagtttttc ttttatcctc ttttgaaaca tctcttttta
      241 taaactctct atcttcaagt ttttttagaa cttttgtcat accaccactt gaaaagattg
      301 tagcatcata aagttctgtt ggagaaagtg aatgattttc attactaaaa taaagtgctg
      361 ctaaaacatc aatatctgaa tgtaacaaat cataactagt tttataaaaa ctctctgatt
      421 cgttataaat atgcttattt gctaaaaaca ttggtaaaga taaagcaaaa acttcatgct
      481 tcttatcttt tgcaactcta ctataaaatt catctatata ttttttattc atagcgaaaa
      541 ttgtatctaa tttaaaatta aaattatctt tctagcaaga caaaattaag taaaaaactg
      601 atagaatttt attttatttc taaaaggagc aaaatgaaaa aaatatattt tactttttta
      661 gtaccaatat tcttatatag tcaaaattta gaagagttag taaatttaac aatagaaaat
      721 agattggttg aatcttctaa acaaaattta gatgcactaa aagatgagta taaaagtgta
      781 caaagaggtt atttaccaaa attagatgct ggtgcttcat actcaataaa tgaacatgaa
      841 tatcctaata atccaaaaaa aagagcaaat gcttatggta gtctgaacta cctattatat
      901 gatggtggga aaaaatatga tatttatgat ggatatgaaa caaatataaa aagtggtgaa
      961 aaatcacttg atgctttaaa aaataatcta tcactaactg ttattcaata ctactttgat
     1021 tatctatctc ttgaagcaaa aaaagatgca aaacaaaaag agatagaaca attaacagca
     1081 caagaagata gaattggaag attttacaat gcaggaacta caacagaaga tgagcttcaa
     1141 aaaatagttt caagacttca aaatgctatt gttgagcttc aagaaattga attaaatatt
     1201 attacaatta cacataatct tgaatacatt acaggcacac aagtatcgat aacagatggt
     1261 tcaaaacttc aagatattaa caatttgata caaaaaaacc ctagatttga tattcaagct
     1321 ttagatttaa gtactcaatc aaaacaaagt attgc
//
LOCUS       DAOQAN010000420         1355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_155603_length_1355_cov_2.039231, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000420 DAOQAN010000000
VERSION     DAOQAN010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_155603_length_1355_cov_2.039231"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /gene="galU"
                     /locus_tag="PKO10_06560"
     CDS             complement(<1..842)
                     /gene="galU"
                     /locus_tag="PKO10_06560"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008336907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HNW66846.1"
                     /translation="MNNTNNPIKKCLFPAAGYGTRFLPATKATPKEMLPVLTKPLIQY
                     GVEEALAAGIENMSIVTGRGKRAIEDHFDISYELEHQIKGTSKEHYLTEIRSVITKCT
                     FSYTRQIEMKGLGHAILCGENLIGNQPFAVLLADDLCDAPDKGVLSQMVELYKKYNCS
                     IVAIEEIPKEDTNKYGVIAGNEIEPGIFMVKDMVEKPEPEVAPSNLAIIGRYILTPDI
                     FDIIRETKPGKGGEIQITDALLTQAKNGMVLAYKFKGERFDCGSIDGFVKATNYFYDK
                     SIKN"
     gene            complement(844..>1355)
                     /locus_tag="PKO10_06565"
     CDS             complement(844..>1355)
                     /locus_tag="PKO10_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HNW66847.1"
                     /translation="INSYGKAIMYKTGHSNLKVKIKETNADFAAEVSGHLFFNDRYFG
                     YDDAIYATFRALELIDAGFDFDKEYEALPQVYSTPEINITVTEDTKFKIIEDLKVALQ
                     NPPSDFPKIKDIITVDGVRVIFEKGWGLVRASNTTPKLVTRFEANNQENAKVYEDALI
                     NLFNKLQGK"
BASE COUNT          401 a          239 c          172 g          543 t
ORIGIN      
        1 tcatttttta tagatttatc ataaaaatag tttgtagctt ttacaaatcc atctatactt
       61 ccacagtcaa atctttcacc tttaaattta taagctaaaa ccataccatt ttttgcttga
      121 gttaaaagtg catcagtaat ttgaatctct ccacctttac ctggttttgt ctctcttata
      181 atatcaaaaa tatctggagt taaaatatat cttccaataa ttgctaaatt tgatggtgca
      241 acttctggtt caggtttttc aaccatatct tttaccatga aaatcccagg ttctatctca
      301 tttccagcaa ttactccata tttatttgta tcctcttttg gtatctcttc aatagcaaca
      361 attgagcagt tatatttttt atatagttca accatttgtg ataaaacacc tttatctggt
      421 gcatcacata agtcatctgc tagaagtaca gcaaatggtt gatttccaat taagttttca
      481 ccacataata ttgcgtgacc taaacctttc atctctattt gtcttgtgta tgaaaaagta
      541 cattttgtaa taacacttct aatctctgtt aaatagtgct ctttactagt ccctttaatt
      601 tggtgttcaa gttcataaga tatatcaaag tgatcttcaa ttgctctttt tcctcttcct
      661 gttacaatag acatattttc aataccagca gctaatgcct cttcaactcc atattgaata
      721 agtggttttg taagtactgg taacatctct tttggagttg cttttgttgc tggtaaaaat
      781 cttgtaccat atccagcagc tggaaataga cattttttga ttggattatt tgtattgttc
      841 atattatttt ccttgtaatt tattaaatag atttattaat gcatcttcat atacttttgc
      901 attttcttga ttattggctt caaatcttgt tactaatttt ggagttgtgt tactagctcg
      961 aactaatccc caaccttttt caaaaataac tcttacacca tcaacagtga ttatatcctt
     1021 gatttttgga aaatctgaag gtggattttg taaagcaact tttaaatctt caatgatttt
     1081 aaattttgta tcttctgtta cagttatatt tatttcaggt gttgagtaaa cttgtggtag
     1141 agcttcatac tctttatcaa aatcaaaacc agcatctatt aactctaaag ctctaaatgt
     1201 tgcataaatt gcatcatcgt atccaaaata tctatcattg aagaatagat gccctgatac
     1261 ttcagccgca aaatctgcat ttgtctcttt aattttaact tttaaatttg aatgcccagt
     1321 tttgtacatg atagctttac cataagagtt tatag
//
LOCUS       DAOQAN010000421         1354 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_155686_length_1354_cov_3.207852, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000421 DAOQAN010000000
VERSION     DAOQAN010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_155686_length_1354_cov_3.207852"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(91..1215)
                     /locus_tag="PKO10_06570"
     CDS             complement(91..1215)
                     /locus_tag="PKO10_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014474191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HNW66848.1"
                     /translation="MIFEFESTNFFLVVAIVLILFATILFFVVEKYKSKIKSLELEKE
                     FLEASIAQNRENQALLKAEIENITSKIFEDNAKKSNQNINQILEPFKAQLNIFGNRVN
                     EVFTEETKQRSSLLTEIKNLKELNNQISNDALNLTKALKGQNKIQGDWGEMILESILN
                     QSGLRRDIEYKVQNSFRTSEGNLVRPDVIVHLPTNKDIIIDSKLSLNSYVDYFNSEDE
                     IEKKESISRLLNSIKNHIKDLSSKKYENIDSLRSLDFVLMFIPIEGAFSIALSQNSNI
                     FEMALKSNIVIVSPTTLYSSLKVIENLWQDNRQNENAKEISKQAADLYDKFVGFLNDF
                     EDIGKSIDKSKESYDKAINKLSSGRGNLISRAEKFFFPIT"
     gene            complement(1212..>1354)
                     /locus_tag="PKO10_06575"
     CDS             complement(1212..>1354)
                     /locus_tag="PKO10_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M48 family peptidase"
                     /protein_id="HNW66849.1"
                     /translation="ALLKLANENKSFPYSHKLYIFFYFSHPPLIERFKELGYDVEKEK
                     FI"
BASE COUNT          438 a          211 c          167 g          538 t
ORIGIN      
        1 tcacctcgga gaatccgggg gatccaggca ggatatcccc ccgtatcttc ccggtaatcc
       61 cgggagagca ggtttggtgg tacaagggac tcaggtgatt gggaaaaaaa atttttctgc
      121 tcttgaaata agatttcctc ttccactact taatttattt attgctttat cataagactc
      181 tttactttta tctatacttt ttccaatatc ttcaaaatca tttaagaatc ctacaaactt
      241 atcatataaa tctgctgctt gttttgaaat ctcttttgca ttctcgtttt gtctattgtc
      301 ttgccaaaga ttttcaataa cttttagact tgaatacaaa gttgttggag aaactataac
      361 aatatttgat tttagagcca tttcaaaaat attactattt tgtgataatg ctatagaaaa
      421 agctccttct ataggaataa acattaaaac aaaatccaag cttcttagtg aatcaatatt
      481 ttcatatttt tttgaactta aatctttaat atgatttttt atagagttta aaagcctaga
      541 aattgactct tttttctcta tttcatcttc actattgaaa taatcaacat aagagtttaa
      601 agataatttt gagtcaatta taatatcttt atttgtaggt agatgaacaa taacatcagg
      661 tcttacaaga tttccttcgc tagttctaaa tgagttctga actttatatt caatatctct
      721 tctaagtcca gattgattta aaatagattc taaaatcatc tctccccaat caccttgaat
      781 cttattttga ccttttagtg cttttgtaag atttaaagca tcattagaga tttgattatt
      841 taactctttt aaatttttta tctctgttaa aagtgaactt ctttgttttg tctcttctgt
      901 aaaaacttca tttaccctat ttccaaatat atttaattga gctttaaatg gttctaaaat
      961 ttgatttata ttttggtttg atttttttgc attatcttca aaaattttag aagtaatatt
     1021 ttctatctca gcttttaata aagcttgatt ttctctattt tgagcgatag aagcttctaa
     1081 aaactctttt tctagctcta aactctttat ttttgattta tacttctcaa ctacaaaaaa
     1141 taaaatagtt gcaaatagta taagaactat tgcaactact agaaagaaat ttgtactttc
     1201 aaattcaaaa atcatataaa tttctctttt tcaacatcat atcccagctc tttaaatctc
     1261 tctatcaaag ggggatgaga gaagtaaaaa aatatgtaaa gcttatgaga atatggaaaa
     1321 gatttattct catttgctag ttttagcaaa gctt
//
LOCUS       DAOQAN010000422         1354 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_155694_length_1354_cov_3.059276, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000422 DAOQAN010000000
VERSION     DAOQAN010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_155694_length_1354_cov_3.059276"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1118
                     /locus_tag="PKO10_06580"
     CDS             <1..1118
                     /locus_tag="PKO10_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012148060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNW66850.1"
                     /translation="QNFHRAPIYTGQIQGSGPRYCPSIEDKVKRFSERERHQLFLEPQ
                     TAKCTEYYINGMSSSLPIEIQKKMIASVKGLENARVIRYGYAIEYDYVDPTELKHTLE
                     TKKIKNLYHAGQINATTGYEEAAAQGLMASINASLAIDNKEPFILRRDEAYIGVLIDD
                     LVTKGTHEPYRMFTSRAEYRLLLREENADLRLSHYGYELGLISKEQIDKVEEKRKTLQ
                     QAVEFMANTWITAKKETLELLESIGEDKINDRVLLIDLIGRNTIDKEKFDVLVPSLKE
                     LDDYFKEQIIIEAKYYRYIQKQQKQIEKMKKMLKLNIPENFSYRGLPGLSNEVVEKLE
                     KHRPPTLYNASLISGITPAALDIIHLNLNMFVISNKK"
BASE COUNT          562 a          152 c          241 g          399 t
ORIGIN      
        1 cacaaaattt tcatagagct ccaatttata caggacaaat tcaaggttca ggtccaagat
       61 attgtcctag tattgaagat aaagttaaaa gatttagtga aagagagaga catcaactat
      121 ttttagagcc acaaactgct aagtgtacag agtattacat aaatggaatg tcaagttcac
      181 ttcctattga aatacaaaaa aagatgatag ctagtgtaaa aggtttagag aatgcaagag
      241 ttattagata tggatatgct attgagtatg attatgttga tccaacagag ctaaaacaca
      301 cacttgaaac aaaaaagata aaaaatcttt atcatgctgg acagataaat gcaacaacag
      361 gatatgaaga agcagcagct caaggtttga tggcaagtat aaatgcatct ttggcaatag
      421 ataataaaga gccatttatt ttaagacgag atgaagcata tattggtgtt ttaattgatg
      481 atttagttac aaaaggaaca catgagccat atagaatgtt tacttcaaga gctgagtata
      541 ggcttctttt aagagaagag aatgcagatt tgagattaag tcattatgga tatgaattag
      601 gattaatctc taaagagcaa atagacaagg tagaagagaa aagaaaaact cttcaacagg
      661 ctgtagagtt tatggctaat acatggataa ctgcaaagaa agagactttg gaacttcttg
      721 aaagtattgg tgaagataaa ataaatgata gagttttatt aatagattta ataggaagaa
      781 acactattga taaagagaaa tttgatgttt tagtacctag tttaaaagag cttgatgatt
      841 atttcaaaga gcaaattatt atagaagcaa aatattatag atatattcaa aagcaacaaa
      901 aacagataga aaaaatgaag aaaatgctaa aactaaatat acctgaaaac ttctcttata
      961 gaggacttcc agggctttca aatgaggttg ttgagaagct tgaaaagcat agaccaccaa
     1021 ctttatataa tgcaagttta atatcgggaa ttacacctgc tgcacttgat attatacatt
     1081 taaatttgaa tatgtttgtc ataagcaata aaaaatgata atggtcaaat ataaattgat
     1141 aatatgtaaa ataaaaaatg ataatgatag gataatttaa tcatgtcaac tagaaaaagt
     1201 atcattccag agtttatgaa gaataatcct gatgatactt ttgaattttt atttcccctt
     1261 aaaatagcac ttgatgaaga taaatatggt aatagtcact atgaagttgt ggttaaaaat
     1321 ataactaaaa atgaagtatt taaatatcca gtat
//
LOCUS       DAOQAN010000423         1352 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_156045_length_1352_cov_2.701619, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000423 DAOQAN010000000
VERSION     DAOQAN010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_156045_length_1352_cov_2.701619"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..143)
                     /locus_tag="PKO10_06585"
     CDS             complement(<1..143)
                     /locus_tag="PKO10_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009294281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HNW66851.1"
                     /translation="MVKIGKYEIKHPIIQGGMGVGISWDQLAGHVSLEGGLGVISAVG
                     TGY"
     gene            complement(153..>1352)
                     /gene="tyrS"
                     /locus_tag="PKO10_06590"
     CDS             complement(153..>1352)
                     /gene="tyrS"
                     /locus_tag="PKO10_06590"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HNW66852.1"
                     /translation="KIKEAIDEIQRGTAEIIDIEAITKLIKKYFETGENFYVKAGFDP
                     TAPDIHLGHTVLIQKLATFQKFGGVVQFLIGDFTATIGDPTGKSETRKVLSRDDVLAN
                     AETYKEQVFKILDPNKTQVVFNSTWLNELGTAGLINLASNLTVARMLERDDFSKRYSS
                     NTPIAVNEFLYPLLQGYDSIALNSDVELGGTDQKFNLLMGRTLQKAYNCKKQQAVLMM
                     PILEGLDGVQKMSKSLGNYIGVTDLPFDMFGKVLSISDELMWRYFELLSSKSLKEIEE
                     IKKGVENGTMHPKKVKENLASEIVDRFHGVGAGALAKAEFEKVFAKKDIPTDIPEFIF
                     EGEIWICQAMLDSKLVDSTSQARRDINSNAVSINQEKISDDKLNLTSGEYILQKGKKS
                     FAKIIIK"
BASE COUNT          406 a          260 c          158 g          528 t
ORIGIN      
        1 tagtatcctg taccaactgc tgaaattact cctaaaccac cttcaagact aacatgtcca
       61 gctaattgat cccaacttat tccaacaccc ataccacctt gaattattgg atgttttatc
      121 tcatattttc ctattttcac aattagcctc ttctatttta ttattatttt tgcaaaactt
      181 tttttgcctt tttgcaaaat gtattcgcca gatgttaagt ttaacttatc atcgcttatt
      241 ttctcttgat taatcgatac cgcatttgaa tttatatctc ttcttgcttg tgaagttgaa
      301 tcaactaatt ttgaatctaa catagcttga caaatccaaa tctcaccttc aaaaataaat
      361 tcaggtatat cagttggtat atcttttttg gcaaaaactt tttcaaattc agcttttgct
      421 aaagctccag caccaactcc atgaaatcta tcaactatct cagatgctaa attctctttt
      481 acttttttag ggtgcattgt tccattttct acaccttttt ttatctcttc aatctctttt
      541 aaacttttac ttgaaagaag ctcaaagtat ctccacataa gttcatctga aattgataaa
      601 acttttccaa acatatcaaa aggaagatct gtaacaccaa tatagttacc taatgatttt
      661 gacatctttt gaacaccatc aagaccttca agaattggca tcattaaaac cgcttgttgt
      721 tttttacagt tatatgcttt ttgcaaagtt ctacccataa gtagattaaa tttttgatct
      781 gttccaccaa gttctacatc gctatttaaa gcaattgagt cataaccttg aagtagggga
      841 tataaaaatt catttacagc aattggagtg tttgaactat atctttttga aaaatcatca
      901 cgttctaaca ttcttgcaac tgtcaagttt gaagctaaat ttataagtcc agctgttcct
      961 agttcattta accaagtact attaaaaaca acttgcgttt tatttgggtc taagatttta
     1021 aaaacttgct ctttgtaagt ctcagcgttt gctaatacat catctctact taaaactttt
     1081 cttgtttcac tttttccagt tgggtctcca atagttgcag taaaatctcc aattagaaat
     1141 tgaactactc ctccaaattt ttgaaaagtt gctaattttt gtattaaaac tgtatgtcct
     1201 agatgaatat caggagctgt tggatcaaat cctgctttta catagaaatt ttcacctgtt
     1261 tcaaaatatt tttttattaa ttttgtaata gcttcaatat caataatctc agcagttcct
     1321 ctttgaattt cgtctatcgc ttcttttatt tt
//
LOCUS       DAOQAN010000424         1351 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_156205_length_1351_cov_2.834877, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000424 DAOQAN010000000
VERSION     DAOQAN010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1351
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_156205_length_1351_cov_2.834877"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..826)
                     /locus_tag="PKO10_06595"
     CDS             complement(<1..826)
                     /locus_tag="PKO10_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HNW66853.1"
                     /translation="MNKIKEFLNSPEIIRLKEIFKLFNNNDNLKLPKTPLNKNDYEYM
                     KSLSAAVVFSSSKKLHWVLISFCITIFLFITWAAFAEIDEIARGSGKVVPNGQNQIVQ
                     NLEGGIVQEILVKEGDIVEKDQVLIRISNEKGTSTAMSNEIKSYYLQAQIKRLEAELK
                     RAPFEYTKGENQELNEFLDNENELYLTNQKQLESKISILKEQIKQKENDLKDAKQTIE
                     HMKFSVGAISKEVTMTKPMVERGIRSQVDFLKLQREESDAKNKLQSVTLSVDKINSE"
     gene            complement(823..>1351)
                     /locus_tag="PKO10_06600"
     CDS             complement(823..>1351)
                     /locus_tag="PKO10_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014474429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNW66854.1"
                     /translation="LIDGIDIAQIDPADLRKGISYVPQDIHLFGGTIKQNILGIHKFI
                     DDEWMLECSKISGTDEFVRMHPAGYDMPLGERGAGLSGGQRQSVGIARALINNSDIWM
                     FDEPTNAMDQTSESIVLNNLMSKVEGKTLLLVTQKMSMVDLVDRIIVMNFGSKVLDGP
                     KDEIINKLGNSGEKQ"
BASE COUNT          410 a          270 c          132 g          539 t
ORIGIN      
        1 tctctgaatt aatcttatca acagataaag taacactttg aagtttattt ttagcatcgc
       61 tctcttctct ttgtaacttt aaaaaatcaa cttgagatct aattcctctt tcaaccattg
      121 gtttagtcat agttacctct tttgaaatag ctccaacact aaatttcata tgctcaatag
      181 tctgttttgc atcttttaag tcattctctt tttgttttat ctgctctttt aatatagata
      241 tttttgactc taactgcttt tgatttgtta aataaagctc attttcatta tctaaaaact
      301 catttaattc ttgattttca cctttagtgt actcaaaagg agctcgcttt aactctgctt
      361 ctaatctttt aatttgagct tgcagataat aagactttat ctcatttgac atagccgtac
      421 ttgtaccttt ttcgtttgaa attctaatca atacttggtc tttttcaact atatcgccct
      481 cttttactaa aatctcctga actattccac cttcaagatt ttgaactatc tgattttgac
      541 catttggaac aacctttcca cttccccttg ctatttcatc aatttcagca aaagcagccc
      601 aagtaataaa taaaaatatt gtaatacaaa aagatattaa aacccagtga agtttttttg
      661 atgagctaaa aacaacagca gcacttaaac tcttcatata ctcataatca tttttgttta
      721 gtggagtttt tggaagtttt aggttatcgt tattattaaa tagtttaaaa atctctttta
      781 atctaattat ttctggacta tttaaaaact cttttatctt attcattgtt tttcaccact
      841 atttccaagt ttatttataa tttcatcttt tggaccatct aaaactttag acccaaaatt
      901 cataactatt attctatcaa ctaaatctac catactcatt ttttgagtaa ctaaaagtag
      961 tgtttttcct tctactttac tcattaaatt atttaaaact atactttcac ttgtctgatc
     1021 catagcattt gtaggctcat caaacatcca aatatccgaa ttatttatca aagctcttgc
     1081 aattcctaca ctttgtcttt gccctccact aagccctgca cctctttcac ctaaaggcat
     1141 atcataacca gcaggatgca ttcttacaaa ctcatctgtt ccacttattt ttgagcactc
     1201 taacatccac tcatcatcta taaatttatg aattcctaaa atattttgtt ttattgttcc
     1261 cccaaaaaga tgaatatctt gcggaacata actaatacct tttcttaaat cagctggatc
     1321 aatttgagct atatctatac catctattaa a
//
LOCUS       DAOQAN010000425         1350 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_156474_length_1350_cov_1.945946, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000425 DAOQAN010000000
VERSION     DAOQAN010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_156474_length_1350_cov_1.945946"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..970)
                     /locus_tag="PKO10_06605"
     CDS             complement(56..970)
                     /locus_tag="PKO10_06605"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNW66855.1"
                     /translation="MRPVLLRHQCPVQPDFCTLQDIKLEFESKIYLYSYTILILENRL
                     KLLNEYQKNLNRLEELYTKLYSYDKVSLNEILNTQISKYDLQMKINELQTTKDNLYLS
                     LEQISYEKIDKIDDSLVLKDINRQQIEEQLIFHPKIQTLQTTSQKYKDTAQLEDAKKF
                     SSITLALEYMQNKEQDYANITMSMPLPIYNTENINKLKANLNTNETNNKLDSQIHNLR
                     LQTKIYLNNLEQYKTNYKILQEKIVPIKQKIQKVLEEFVGFDKESLKENLTSLNELID
                     YEMKASEQLEKYFENYSELIYYSNKGVK"
     gene            1121..>1350
                     /locus_tag="PKO10_06610"
     CDS             1121..>1350
                     /locus_tag="PKO10_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66856.1"
                     /translation="MEKIAEIIRLHFKLDLSVRQTSSALNVSRSSVGDYCQKFKYLNL
                     DVDDFLKLSIEKQEELLFPKTPNISQLDNKKVL"
BASE COUNT          449 a          158 c          179 g          564 t
ORIGIN      
        1 attggattgg aattaagagt tatagctata agtattatag ttaagaatat ttttttcatt
       61 ttacaccttt atttgagtaa tatataagtt cactgtagtt ctcaaaatat ttttcaagtt
      121 gttcacttgc tttcatttca taatcaatca attcatttaa actagttaga ttctctttta
      181 aactctcttt atcaaatcct acaaactctt ctaaaacctt ttgtattttt tgttttatag
      241 gtacaatctt ttcttgaagg attttataat ttgttttata ttgctctaaa ttatttagat
      301 atatttttgt ttgtaatctt aaattatgaa tttgactatc tagtttatta ttagtttcat
      361 ttgtatttaa atttgctttt aatttattaa tattttctgt attataaatt ggtagaggca
      421 tagacattgt aatatttgca taatcttgct ctttattttg catatattct aaagctaaag
      481 ttatacttga aaactttttt gcgtcttcaa gttgtgccgt atctttatat ttttggcttg
      541 ttgtttgaag tgtttgtatc tttggatgaa atattaattg ttcttcaatt tgttgtctgt
      601 ttatatcttt taatactaag ctatcatcaa ttttatcaat cttctcataa cttatttgct
      661 ctaaacttag atataaatta tcttttgttg tttgaagttc atttattttc atttgtaaat
      721 catattttga aatttgtgta tttaatatct catttaaaga tactttatca tatgaatata
      781 gttttgtata aagctcttct agccgattta aatttttttg atattcattt agcagtttta
      841 atctattttc taaaattaaa atagtgtatg aatatagata aatttttgat tcaaattcta
      901 gctttatatc ctgtagcgtg caaaaatcgg gctgaaccgg acactgatgt cggagtaaga
      961 ccggacgcaa aaacggagtt caaccggaca gtaaaacggt cttaagccgg acactaaaag
     1021 gtaaaaaccg ataattaaga ttaaattcag caaatgagat tttctaaatt tatatacttc
     1081 aaaaatagaa ttttttggag tagaaatgag gaaaataaaa atggaaaaga ttgcagaaat
     1141 tattagactg cattttaaat tagatttgtc agtaagacaa acatcaagtg cattaaatgt
     1201 atccagaagt agtgttggtg attattgtca aaagtttaaa tatctaaatt tagatgttga
     1261 tgattttcta aaactaagta ttgaaaaaca agaagaatta ctctttccaa agactccaaa
     1321 tatctctcaa ttagataata aaaaagtact
//
LOCUS       DAOQAN010000426         1347 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_157031_length_1347_cov_2.272446, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000426 DAOQAN010000000
VERSION     DAOQAN010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1347
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157031_length_1347_cov_2.272446"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..595
                     /locus_tag="PKO10_06615"
     CDS             23..595
                     /locus_tag="PKO10_06615"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HNW66857.1"
                     /translation="MKKLFLIIGAPGSGKTTDAELIAKRHENITHYSTGDMLRAEMAK
                     NSDIGKEIAKYVNAGNIVPIKIAIQTIVNAIKNAPTPTIIIDGYPRSLEQMSALDEYL
                     KNEKELELLSCIEVVVSEEVAKDRVLGRNRGDDDKVEVFNNRMSVYIEPLKAIQDFYE
                     SKNILKKIDGERTIEEIVDEMESFILSKIV"
     gene            611..>1347
                     /locus_tag="PKO10_06620"
     CDS             611..>1347
                     /locus_tag="PKO10_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HNW66858.1"
                     /translation="MKKRVNFYSVIIGTELLNGRRKDAHFAFLNQELLKRGWEHKASF
                     VIEDDKELMLKIFNLIKSDEHSVMFCFGGIGATPDDFTRQIAADAFTNSQMEFHEEAK
                     KRIENRFKDEAYPHRVNMAYLPINAKLLKNVVNDVAGFYLEDRFFFTPGFPSMSQSMV
                     LEALDRHYTKSNIKKYRKTVTIVSSENDLIEVMKKIPTHLEFSSLPKIIGEERRVVIS
                     VAGYNQDEVNFAFKLFLDFCLENKKEYV"
BASE COUNT          543 a          139 c          269 g          396 t
ORIGIN      
        1 cttgaaaaat aaaaggaaat gaatgaaaaa actattttta attattggag cacctggaag
       61 tggaaagaca acagatgctg aacttatagc aaaaagacat gaaaatataa ctcactatag
      121 tacaggcgat atgctaagag ctgagatggc aaaaaatagt gatataggaa aagagatagc
      181 aaaatatgta aatgctggaa atatagtacc aattaaaata gctatacaaa ctatagtaaa
      241 tgctataaaa aatgctccaa cacctacaat aataatagat ggatatccta gaagtttaga
      301 gcagatgagt gctttggatg agtatttgaa aaatgaaaaa gagttagagc tactatcttg
      361 tatagaggta gttgtaagcg aagaagttgc aaaagataga gtgcttggaa gaaatagggg
      421 agatgatgat aaggttgaag tatttaacaa cagaatgagt gtgtatatag agcctttaaa
      481 agctattcaa gatttttatg aatcaaaaaa tattttgaaa aaaatagatg gtgagcgaac
      541 tatagaagag attgtagatg agatggaaag tttcattttg tcaaaaatag tttaaaaaag
      601 attttaaaca gtgaaaaaga gagtaaattt ttatagcgta attattggaa ctgaactttt
      661 aaatggcaga agaaaagatg ctcactttgc atttttaaat caggagcttt taaagcgtgg
      721 ttgggagcat aaagcatctt ttgtgattga agatgataaa gagctgatgc taaaaatatt
      781 taatcttata aaatctgatg agcatagtgt gatgttttgt tttggtggaa ttggagcaac
      841 tccagatgat tttacaaggc aaatagctgc agatgctttt acaaactcac aaatggagtt
      901 tcacgaagag gctaaaaaaa gaatagaaaa tagatttaaa gatgaagcct atcctcacag
      961 agtaaatatg gcgtatcttc caataaatgc aaaacttcta aaaaatgttg taaatgatgt
     1021 agctggattt tatttagaag atagattctt ttttacacca ggatttccat ctatgagtca
     1081 aagtatggtt ttggaggctt tggatagaca ctatacaaaa tcaaatatca aaaaatatag
     1141 aaaaacagta acaattgtaa gtagtgaaaa tgacttaata gaagttatga aaaaaattcc
     1201 aacacacctt gagttttcat ctttgccaaa gattattggt gaagaaagaa gagttgtaat
     1261 atctgttgct ggatataatc aagatgaggt aaattttgct tttaaactct ttttggattt
     1321 ttgtcttgaa aataaaaaag agtatgt
//
LOCUS       DAOQAN010000427         1346 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_157185_length_1346_cov_2.443842, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000427 DAOQAN010000000
VERSION     DAOQAN010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157185_length_1346_cov_2.443842"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1346
                     /locus_tag="PKO10_06625"
     CDS             <1..>1346
                     /locus_tag="PKO10_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002811340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNW66859.1"
                     /translation="LRDLDKDTVNFVPNYDDTMKEPAVLPTRVPTLLLNGSEGIAVGM
                     ATKIPPHNLGELLDAILHLIDNPSAEAIDLMEFIQGPDFPTGGTIFGRRGIVDAYNTG
                     RGRVRIRAKHHIESKAKKDVIVIDELPYQVNKARLIELIANLAKDKQIDGIAEVRDES
                     DREGIRVVIELKKEAMAEIVLNNLYKSTPMETTFGIILLAVYNKEPKVFNLPEILNIF
                     LSHRKTVIIRRTIFDLEKAKARAHILEGLKIALDNIDEVVKIIRASANDTEAKDNLSS
                     RFGLSAIQSQAILDMRLGRLTGLQRDKLEAEYAELMLLIAELESILRSEEKLNEIIKE
                     ELNEIKEKFSTPRKTEIEDSYDEIDVEDLIPNEPMVVTITHNGYVKRVPIKSYERQKR
                     GGKGKVAVTTHDDDFIERFFVSNTHDTLMFVTNMGQLYWLKVYKIPEGSRTAKGKA"
BASE COUNT          540 a          153 c          259 g          394 t
ORIGIN      
        1 ttttaagaga tttagataaa gatactgtaa attttgttcc aaactatgat gacactatga
       61 aagaaccagc tgttcttcct acaagggttc caactcttct attaaatgga agtgaaggaa
      121 ttgcagttgg aatggcaaca aaaattccac ctcataatct aggggagctt ttagatgcaa
      181 ttttacattt aattgataat ccaagtgctg aagcaataga tttaatggag tttattcaag
      241 gtccagattt tccaactggt ggaacaattt ttggaagaag aggaattgtt gatgcatata
      301 atactggacg tggaagagtt agaattagag caaaacatca tatagaatca aaagcaaaaa
      361 aagatgttat tgttattgat gagttaccat accaagtaaa taaagcaaga ttaattgaac
      421 ttattgcaaa cctagcaaaa gataagcaaa ttgatggaat tgcagaagtt agagatgagt
      481 ctgatagaga aggaattaga gttgtaattg agctaaaaaa agaggcaatg gctgaaatag
      541 ttcttaataa tctttataaa tcaactccta tggagacaac atttggaatt atattgcttg
      601 cagtttacaa taaagagcca aaagtattta atcttccaga aattttaaat attttcttat
      661 cacatagaaa aactgtaatt attagaagaa caatatttga tttagagaaa gcaaaagcaa
      721 gagcacatat tttagaaggt ttaaaaattg ctctagataa tattgatgaa gttgtgaaaa
      781 ttattagagc tagtgcaaat gatacagaag caaaagataa tttatcttca agatttggtt
      841 taagtgcaat tcagtctcaa gcaatattgg atatgagatt gggaagatta acaggtcttc
      901 aaagagataa acttgaagct gagtatgctg agcttatgtt attaatagca gaattagagt
      961 caattttaag aagtgaagag aaactaaatg agattataaa agaggagtta aatgagataa
     1021 aagaaaaatt ctcaactcca agaaaaacag aaattgaaga ctcttatgat gaaattgatg
     1081 ttgaagattt aattccaaat gagccaatgg ttgtaacaat aactcataat ggatacgtaa
     1141 aaagagttcc tattaagtct tatgagagac aaaaaagagg tggaaaaggt aaagttgcag
     1201 taacaactca tgatgatgac tttattgaga gattctttgt aagtaatact cacgatacat
     1261 tgatgtttgt tacaaatatg ggacaacttt actggttaaa agtttacaaa attccagaag
     1321 gaagtagaac tgcaaaagga aaagct
//
LOCUS       DAOQAN010000428         1342 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_157822_length_1342_cov_3.248640, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000428 DAOQAN010000000
VERSION     DAOQAN010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157822_length_1342_cov_3.248640"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1342)
                     /locus_tag="PKO10_06630"
     CDS             complement(<1..>1342)
                     /locus_tag="PKO10_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HNW66860.1"
                     /translation="IKHHYSIYLKMQRKGITIEEVLDLFAIRILVPSDIDCYKALGHI
                     HLEFKPLVSRFKDYIATPKENGYKTIHTTVFYNSKIYEIQIRSFDMNSVAEYGIAAHW
                     AYKNGEKNSSSSSNLNWLKSLEFSNDNIEEFYNETKENLFNDEIIVYSPKGEVFILPI
                     GSTAYDYAFAVHTNVGKKAISCYINKIKKPLLTVLKSTDIIQIELGNEPIIRCSWIDM
                     VKTSRAKKQLKFLCMQRQREIDELAGKNIINTIFSRYYNDILEHYPVKGLYKVPTNLP
                     FLKNAKQIVEKKVLKEKGIMGRFKIFTSKIKEFKFDNMLIYSNFNINSVSFDHCCHPK
                     FGDDIIAFRNSHNAIIHHKMCDKAYSKIKENEQMLYCEWAKNSVFQYKMLISIPNTKG
                     ELAKVLAYLSKSDFYILACNFGRQKHSYIQYCDIEFEINNSNVDEVRQIVEKNIK"
BASE COUNT          440 a          196 c          152 g          554 t
ORIGIN      
        1 ttttatattt ttctcaacaa tttgtctaac ttcatctaca tttgaattat ttatctcaaa
       61 ctctatatca caatattgaa tatatgagtg tttttgtcgc ccaaaattac aagctaagat
      121 ataaaaatca cttttagaaa gatatgctaa aacttttgct aattcacctt ttgtatttgg
      181 aatacttatt agcattttgt attgaaaaac agagtttttt gcccattcac aatacaacat
      241 ttgttcattt tcttttattt tagaataagc tttatcacac attttatggt gaattattgc
      301 attgtgagag tttctaaatg caataatatc atctccaaat tttggatgac aacagtggtc
      361 aaatgataca gaatttatat taaagttaga ataaataagc atattgtcaa atttaaactc
      421 ttttatcttg cttgtgaaga ttttaaatct tcccataatt cctttctctt ttaatacttt
      481 tttctctaca atttgttttg cattttttaa aaaaggtaaa tttgttggta ccttatatag
      541 accttttact ggataatgtt ctaaaatatc attataatat ctagaaaata tagtatttat
      601 aatatttttt ccagcaagtt catctatctc tctttgtctt tgcatacata aaaattttaa
      661 ctgttttttt gctcttgaag tttttaccat atctatccaa gaacatctta tgattggttc
      721 atttcctagc tcaatttgta taatatctgt actttttaaa acagttaaaa gaggtttttt
      781 aattttattt atataacaag aaattgcttt ttttccaacg tttgtatgaa ccgcaaaagc
      841 ataatcataa gcagttgaac caataggcag gataaaaact tcaccttttg gtgaataaac
      901 tataatttca tcgttaaaca aattttcttt tgtttcatta tagaactctt ctatgttgtc
      961 attagagaac tccaaagatt ttaaccaatt aagatttgaa cttgaagagc tatttttttc
     1021 tccattttta tatgcccagt gagctgctat tccatactct gcaacactat tcatatcaaa
     1081 tgaacgaatc tgaatctcat atatttttga attataaaaa actgttgtat gaatagtttt
     1141 gtaaccattt tctttaggtg ttgctatata atctttaaat cttgaaacta aaggtttaaa
     1201 ttctaaatga atatgtccta atgccttata gcaatctata tcgcttggaa ctaagattct
     1261 aattgcaaaa agatcaagta cctcttctat agtaatacct tttctttgca tttttaaata
     1321 tattgaatag tgatgtttaa tt
//
LOCUS       DAOQAN010000429         1342 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_157831_length_1342_cov_3.121212, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000429 DAOQAN010000000
VERSION     DAOQAN010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157831_length_1342_cov_3.121212"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(77..556)
                     /locus_tag="PKO10_06635"
     CDS             complement(77..556)
                     /locus_tag="PKO10_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014473547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNW66861.1"
                     /translation="MNLLEFIDKGGIIVYILIFLNIIGFTIIIWKFTTLPQVNKTIAK
                     IASRLDFNHSINAQIEYEVKKLESGLVIIKNIAIISPLLGLLGTVVGIYSSFEEITIK
                     GLGDPTIFSGGIAVALITTIAGIIVSIPHQIAYNHFISLVDKIEIKAKKELVKEENR"
     gene            617..>1342
                     /locus_tag="PKO10_06640"
     CDS             617..>1342
                     /locus_tag="PKO10_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014473228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-aminocyclopropane-1-carboxylate deaminase"
                     /protein_id="HNW66862.1"
                     /translation="MKFTNSKTSEIFLNNQRYFVKRDDLLHSDFSGNKARKFYYFLKN
                     DLQGIEKIISHGSNQSNAMYSLSVLCKLKNLKFDYYVSHLPDILSKNPNGNYKEALKN
                     GMNLIIGEIPQHFKENELFISEGGAVKEASFGIEILANEIKSWAKEQNIEDKRLKVFL
                     PSGTGTTALYLQKFLPFEVFTCSCVGDDEYLKKQFLALEEKNHPKILKKEKKYHFGKL
                     YIEFYLKHLELLKQTNIEFDLLYD"
BASE COUNT          519 a          173 c          177 g          473 t
ORIGIN      
        1 actataaaaa atattaaaag aataaaaaca acatcaataa ttggtgttaa atcaagtccc
       61 aaatgctctc ttcgtttcat ctattctctt cttttactaa ctctttttta gcttttatct
      121 ctattttatc aacaaggcta atgaagtgat tataagctat ttgatgagga attgatacaa
      181 taatcccagc aattgttgta attagtgcaa ctgcaattcc acctgaaaat attgtaggat
      241 ctcctaaacc ttttatagtt atctcttcaa atgagctata aattccaaca acagttccta
      301 aaagtcccaa aagtggagag attattgcaa tattttttat aattacaagc cctgattcta
      361 atttttttac ttcatactca atttgtgcat ttattgagtg gttaaaatcc aatcttgaag
      421 ctatttttgc tattgtttta tttacttgtg gaagagttgt aaatttccaa ataattattg
      481 taaatccaat aatatttaaa aaaattaaaa tatagactat aattccaccc ttgtctataa
      541 attctaataa gttcatatga aagccttttg aaatttttgg atattatatt gaaaaattat
      601 ttacaaatag gtatttatga aatttacaaa ctcaaaaaca tctgaaattt ttttaaacaa
      661 tcaaagatat tttgtaaaaa gagatgattt acttcatagt gattttagtg gaaataaagc
      721 tagaaaattt tactactttt taaaaaatga tttacaaggt attgaaaaaa ttatatctca
      781 tggctcaaat caatcaaatg caatgtactc actatctgtt ttatgtaaat taaaaaatct
      841 aaaatttgac tactatgtat cccatttacc agatatttta tctaaaaatc caaatggaaa
      901 ctacaaagaa gcacttaaaa atggtatgaa tttaattatt ggtgaaattc cacaacattt
      961 taaagaaaat gagttattca taagtgaagg aggagctgtt aaagaggcta gctttggaat
     1021 agaaatatta gcaaatgaga taaaatcttg ggcaaaagag caaaatattg aagataaaag
     1081 attaaaagta tttttaccaa gtggaacagg aacaactgct ttatatttac aaaagtttct
     1141 accttttgaa gtctttactt gctcttgcgt aggagatgat gagtatctaa aaaagcagtt
     1201 tttagctttg gaagaaaaaa atcatccaaa aattttaaaa aaagagaaaa aataccattt
     1261 tgggaaactc tatattgagt tttatttgaa acatttagag cttttaaaac agacaaatat
     1321 agagtttgac ctgctttatg at
//
LOCUS       DAOQAN010000430         1340 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_158281_length_1340_cov_2.373541, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000430 DAOQAN010000000
VERSION     DAOQAN010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1340
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_158281_length_1340_cov_2.373541"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="PKO10_06645"
     CDS             complement(<1..194)
                     /locus_tag="PKO10_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66863.1"
                     /translation="MINENRVLNEANSLFTQRKFDKALFLYSLLISNFPNNEEYPIYA
                     LLCDISIEDENKAIALFDYF"
     gene            complement(201..476)
                     /locus_tag="PKO10_06650"
     CDS             complement(201..476)
                     /locus_tag="PKO10_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HNW66864.1"
                     /translation="MFPFTDEDLQEPVNNIIEKKISPMLARDGGAIELLDIKNAKVYI
                     QLKGACVGCSASGSTLKYVVEKELKSAIHPDLKIINVPIGKENYLED"
     gene            complement(593..1162)
                     /locus_tag="PKO10_06655"
     CDS             complement(593..1162)
                     /locus_tag="PKO10_06655"
                     /inference="COORDINATES: protein motif:HMM:NF019869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66865.1"
                     /translation="MYLDVCKDRLYCDDKNDIHRTASQTAMAMITKKLISTLACILTY
                     TMDELLDYAPKIVKGDAKDIFDFEKYNLPDVKSEIKDELFVEAKEKFSEIKDSLSKEK
                     IIKSTLELEIVTDNKEFLALDEVESSDWFLVSKLSKITSSKELLGSFKLEDIEFKVYK
                     ASGHKCPRCWKYTSTKEEALCSRCEEVVK"
BASE COUNT          425 a          258 c          163 g          494 t
ORIGIN      
        1 aaaaagtaat caaacaaagc tattgccttg ttttcatctt caattgaaat atcacacaaa
       61 agtgcatata taggatactc ttcattattt ggaaaatttg aaatcagcaa tgagtataaa
      121 aaaagcgctt tatcaaattt tctttgcgta aaaagtgaat ttgcttcatt taaaactcta
      181 ttttcattta tcatttatat ttaatcctct aaatagtttt cttttcctat tggaacatta
      241 attattttta aatctggatg tatggcactt tttaactctt tctcaacaac atattttagt
      301 gtacttccac ttgcgctaca tccaacacaa gcacctttta actgaatata aacttttgca
      361 ttttttatat ccaacaactc aattgctcca ccatcacgtg caagcatagg agaaattttt
      421 ttttctataa tattgtttac aggttcttgt aaatcttcat ctgtaaatgg aaacattttt
      481 taatccttct ttttttcata cctttttaca aaaaatattc caatagcact aagagctaaa
      541 ataactccta tagtttggaa tataaatgta agagtaactt cattttcaaa cattatttaa
      601 caacttcttc acatctagaa catagtgctt cttcttttgt tgaagtatat ttccaacatc
      661 tagggcattt atgtccactt gccttataaa ctttaaactc gatatcttca agtttgaaac
      721 ttcctaatag ttctttactt gaagttatct ttgataattt actaactaaa aaccaatcac
      781 ttgattctac ctcatctaag gctaaaaact ctttattatc tgtaactatc tctaactcta
      841 aagtagattt tataattttt tctttactta aagaatcttt tatttcagaa aacttctctt
      901 ttgcttctac aaacaactca tcttttattt cagattttac atctggaaga ttatattttt
      961 caaaatcaaa aatatctttt gcatcacctt ttactatttt aggagcataa tctaaaagtt
     1021 cgtccatagt ataagttaaa atacatgcca atgttgaaat tagttttttt gtaatcatag
     1081 ccatagctgt ttgagatgca gttctatgaa tatcattttt atcatcacaa tatagtctat
     1141 ctttgcaaac atcaagataa attcctgata aatcaaagta ttaacgtgca gcaccggtgg
     1201 cacgcatcgt gcaattggag gatgccggag ccaagctccg ctcaaacaat aacccggagg
     1261 agaaaccgat gcgcactgtc ctgtccctgg ccgccgcctt gaccctgacc gtcccgctgg
     1321 ccgcccatgc cgttgatgtg
//
LOCUS       DAOQAN010000431         1339 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_158547_length_1339_cov_1.898754, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000431 DAOQAN010000000
VERSION     DAOQAN010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1339
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_158547_length_1339_cov_1.898754"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(189..1265)
                     /locus_tag="PKO10_06660"
     CDS             complement(189..1265)
                     /locus_tag="PKO10_06660"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNW66866.1"
                     /translation="MRFFDELSYIKYKLGIGKILITIILIYILVIVLDSFLVHFTIKS
                     ISNDAALINSSGKIRGGIQRAVKLTLQDRYSNENILIIDKIFKDFSSSYEKSTSKDIM
                     KDFYIKLKELEYSWSDLKILLSAYDKNKTFDLKEKILDKSEEAWALSIETLDIAQKLY
                     DEKIGSFLSIFIIFTINLFFGFIIIYIINKSVRLNLEVISAIDTLTNIGNRNRYNEAI
                     DIYLNLFKKYNKKIAYILLDIDFFKSINDNFGHDKGDDVLIKSTKVILTNIKKDDLFC
                     RVGGEEFVIVSENINTKDELEKFANKIRTSVENFDFELERKVTISLGATFIEKDDTKH
                     SLFKRADEALYISKNSGRNRVTIL"
BASE COUNT          487 a          189 c          100 g          563 t
ORIGIN      
        1 ttttcttttg ggtagatatt taagacttcg tcttttgttt caaaaatata ctcttttttt
       61 aactctttat atgacaaata gttaaataaa atatttatca taaaaacaaa aagtagaaaa
      121 agagtaaaaa atagactatt ttttgatatt tgtaaatttt gaataatcat aatagattat
      181 acaaaatttt aaagaattgt caccctattt cttccactat tttttgatat atataaagct
      241 tcatcggctc ttttaaacag gctatgtttt gtatcatctt tttcaataaa agtagctcca
      301 agacttattg taacttttct ttcaagctca aaatcaaagt tttcaacact tgttcttatt
      361 ttatttgcaa atttttctag ttcatctttt gtatttatat tttctgaaac tattacaaac
      421 tcttctccac caactctaca aaataaatca tcttttttaa tatttgttaa tatcaccttt
      481 gtagatttaa ttaaaacatc atcgccttta tcatgtccaa agttatcatt tatgctttta
      541 aaaaaatcta tgtcaagtaa tatatatgct atctttttat tatacttttt aaacaaattt
      601 agataaatat caatagcttc gttatatcta tttctatttc ctatatttgt aagagtatca
      661 attgccgaga taacttccaa atttaatcta acacttttat ttataatata aataattata
      721 aaaccaaaaa ataaatttat tgtaaaaatt ataaatatag ataaaaaaga gcctattttt
      781 tcatcatata atttttgagc aatatctaaa gtttcaattg aaagagccca agcttcttca
      841 cttttatcta aaattttctc tttcaaatca aaagttttat ttttatcata tgctgataat
      901 aaaatcttta aatcactcca agagtactcc aactctttta gttttatata aaaatctttc
      961 ataatatctt ttgaagtaga tttttcataa gagcttgaaa aatctttaaa tatcttatct
     1021 attattaaaa tattctcatt actatatcta tcttgtagag ttaatttaac agctctttga
     1081 attccacctc ttattttacc actactattt atcaaagcag catcattact tatacttttt
     1141 attgtgaaat gtacaagaaa agagtctaaa acaattacta aaatatatat taaaattatt
     1201 gttattaata tttttccaat tcctaattta tattttatat agctcaattc atcaaaaaat
     1261 ctcatcttta cccttagcga tattttttat taacctaaat taaatttaaa gccaaatatt
     1321 atcatttttt atattaaga
//
LOCUS       DAOQAN010000432         1337 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_158805_length_1337_cov_3.099064, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000432 DAOQAN010000000
VERSION     DAOQAN010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_158805_length_1337_cov_3.099064"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="PKO10_06665"
     CDS             complement(<1..216)
                     /locus_tag="PKO10_06665"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HNW66867.1"
                     /translation="MNKELEFENSIKNILNYIDEDVTREGLLDTPKRVRKAYEFMFSG
                     YKLDPKEIIQKALFTSTNDEMVVVKDIE"
     gene            complement(241..1191)
                     /gene="corA"
                     /locus_tag="PKO10_06670"
     CDS             complement(241..1191)
                     /gene="corA"
                     /locus_tag="PKO10_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014474854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HNW66868.1"
                     /translation="MINCYVKKQHRLTVVEGVEYLNDIEDRKSVIWIDMLFPTIEEVK
                     AIEGIFNIEFPTKQESEEIELSSRYWEEGNRIEINSYFLINDKKEPVNETVSFILQDE
                     LLISVRYKKLASFDASIKKLLASPREYKTGYSIFSQIIDIRIDADADIIENLGRDIAA
                     IRKQAFNDDVENEDLLEQMSAFENLNMKIRENLTDKQRILNSLLKSQKISDDKSELPI
                     MLKDIRSLIDHTNFNFERLDYLQNIFVGLLSVEQNKVIKIFTIVNVIFLPPTLVASIY
                     GMNFEIMPELSWDYGYLFSVGIMILSAITPLIIFRKKGWI"
BASE COUNT          466 a          239 c          132 g          500 t
ORIGIN      
        1 ctcaatatct tttacaacaa ccatttcatc atttgtagat gtaaaaagtg ctttttgaat
       61 aatctctttt gggtcaagtt tatatccact aaacataaac tcataagctt ttcgaactcg
      121 ctttggagta tccaaaagtc cttctcttgt aacatcttca tcaatataat ttaaaatatt
      181 ttttattgaa ttttcaaact ctaactcttt attcataata tattattcct tttaaaaatt
      241 tcatatccaa cctttctttc taaagattat caaaggtgta atagctgata aaatcataat
      301 accaactgaa aataaatatc cataatccca gctaagctca ggcataatct caaagttcat
      361 accataaata ctagcaacta gcgtaggagg taggaaaata acatttacga tagtaaagat
      421 ttttataacc ttattttgct caacacttag aagtcccaca aaaatatttt gcaaataatc
      481 aagtctctca aaattaaaat tagtgtgatc aatcaaagac ctaatatcct ttaacataat
      541 aggaagttca cttttatcat ctgaaatctt ttgtgatttt aacaaagagt ttagaattct
      601 ttgtttatct gtaagatttt ccctaatttt catatttaag ttttcaaatg ctgacatctg
      661 ttctaataaa tcttcatttt ctacatcatc attaaatgcc tgttttctaa tagctgcaat
      721 atctcttcct aaattttcta taatatcagc atctgcatca attctaatat ctataatttg
      781 tgaaaatata gagtatcctg ttttatactc tcttgggctt gctagaagtt ttttaattga
      841 agcatcaaaa cttgctagtt ttttatatcg aacagatatt aaaagttcat cttgtaaaat
      901 aaaagaaacc gtctcattta caggctcttt tttatcattt ataagaaaat aactatttat
      961 ctctattcta tttccttctt cccaatacct agaactaagc tcaatctctt cactctcttg
     1021 ttttgtagga aactcaatat taaaaatacc ttcaatagct tttacctctt caatagtagg
     1081 gaaaagcata tcaatccaaa taacactttt tctatcctct atatcattta gatattcaac
     1141 accttcaact acagttaatc tgtgttgttt ttttacataa caattaatca aggttcctcc
     1201 tttatctaat catacttccc atatcccaaa tcggcgtaaa tattgcaaga actataaaaa
     1261 gagttaagct tgccattaat agaagaaata tgggctgtat caataaaatt ataaaattta
     1321 tactatcatc aaatcta
//
LOCUS       DAOQAN010000433         1337 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_158870_length_1337_cov_2.216849, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000433 DAOQAN010000000
VERSION     DAOQAN010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_158870_length_1337_cov_2.216849"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1337)
                     /locus_tag="PKO10_06675"
     CDS             complement(<1..>1337)
                     /locus_tag="PKO10_06675"
                     /inference="COORDINATES: protein motif:HMM:NF012973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HNW66869.1"
                     /translation="EFVVGGNMVIGVVIFIILVLINFMVVTKGATRVAEVTARFTLDS
                     MPGKQMAIDADLNAGFIDDKEAQERRKALITEANFYGAMDGSSKFVKGDAIAGIIITV
                     VNIVGGLLVGIFQHDLPASEAASIYTILTIGDGLVAQIPALLTSTATAIIITRSNTDE
                     ERFATRAVNQLLKDTKSLGLVGIGLMLFGLVPGFPTGILMTMGLMISALGYTIIMIDK
                     GEDNIVTRFFKPQVPKKIEKPEDLKEKKRAAAVPDESQSIETVMKLEVLELKLGIRLL
                     QLVQGNSELLDKIKAIRKNIASELGFIIPQIRISDDTTLGANEYQFYLKRIPIVKGRI
                     EVDKLLAMGGLSSEHLDGIKVKEPVFNLDAVWISKDLKEDALMKGFTVVDAPTIISTH
                     ISEIIRKYAEDIITRQDVVDIVERLKKDFPIVVEEAMKVASYGSLLKVCKDLL"
BASE COUNT          433 a          271 c          154 g          479 t
ORIGIN      
        1 aagcaaatct ttacaaactt ttaacaaact tccatatgaa gcaactttca tagcttcttc
       61 aacaacaata gggaaatctt ttttcaatct ctctacaata tcaacaacat cttgtcttgt
      121 aataatatct tctgcatatt ttctaataat ttctgatata tgagttgata taattgttgg
      181 agcatctaca actgtaaacc ctttcatcaa agcatcttct tttaaatctt ttgaaatcca
      241 aacagcatct agattaaata caggctcttt tacttttatt ccatcaagat gctcacttga
      301 taatccaccc atagcaagaa gtttatctac ttctattctt ccttttacta ttggaattct
      361 ttttagataa aattgatact catttgctcc taaagttgta tcatcagaaa ttcttatttg
      421 aggtattata aatcctaact cagaagctat attttttctt atcgctttta ttttatcaag
      481 aagttctgag ttaccttgaa ccagttgtaa aagtctaatt ccaagtttta actccaaaac
      541 ttcaagtttc attacagttt ctatactttg actctcgtca ggaacagctg ctgctctttt
      601 cttctctttt aaatcttctg gtttttcaat ctttttagga acttgtggtt taaaaaatct
      661 tgtaacaata ttatcttcac ccttatcaat cataataata gtgtaaccta aagctgatat
      721 cataagtccc atagtcatca aaattcctgt agggaaacct ggaactagtc caaaaagcat
      781 tagaccaata cctactaaac caagagattt tgtatctttt aaaagttgat ttacagctct
      841 tgttgcaaat ctctcttcat ctgtatttga tcttgttatt ataatagcag ttgcagttga
      901 tgttaaaaga gctggaattt gtgcaacaag tccatctcca atagttaaaa ttgtgtatat
      961 acttgcagct tcactagcag gtaaatcgtg ttgaaaaata ccaaccaaaa gtccaccaac
     1021 aatatttaca actgtaataa taataccagc aattgcatca ccttttacaa atttagatga
     1081 cccatccatt gctccataga agtttgcttc tgttataagt gcttttcttc tctcttgcgc
     1141 ctctttatca tcaataaatc cagcatttaa atctgcatca atagccattt gtttaccagg
     1201 cattgaatca agtgtaaatc tagcagttac ctcagcaacc cttgtagcac cttttgttac
     1261 aaccataaag tttatcaaaa ctaaaattat gaaaataaca accccaataa ccatatttcc
     1321 gccaacaaca aactctc
//
LOCUS       DAOQAN010000434         1333 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_159567_length_1333_cov_2.506260, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000434 DAOQAN010000000
VERSION     DAOQAN010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1333
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_159567_length_1333_cov_2.506260"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1333
                     /locus_tag="PKO10_06680"
     CDS             <1..>1333
                     /locus_tag="PKO10_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014472873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNW66870.1"
                     /translation="DVHSVIKNVKAKLHSLNIDDVEIVEVYDRTSLIDAAIDTLKRTL
                     IEESIIVMLIVAIFLFHFRSALIIIITLPLTVMISFLLMKLFNIGSNIMSLGGIAIAI
                     GAMVDATIVMVENAHKHLQGKENITKEERIEIILSSAKQVGRPIFFALLLVVVSFLPI
                     FALSGQEGKLFSPLAFTKTFAMLCGAMLSITLVPILMIYFIKGKIIQEDKNILNSFFI
                     KIYSPILRLSLKLKYLVVTIFFIIIIFALYTYSKQKWEFMPTLNEATFMYMPVTPYGI
                     GADLAKQLAQKTNMVIKSFPEVQNTFAKAGRAATATDPAPLAMIETIITFKPQSQWRE
                     GMTYKKLIDEMDKKLQIPGLINTWTYPIKGRIDMLLTGIRTPLGIKLYGKDLEVLQEE
                     SSKIENILKKYEGSMSVSADKINQGYYLNIKIDDEEISRYNISKNTILETLS"
BASE COUNT          508 a          159 c          214 g          452 t
ORIGIN      
        1 tgatgttcat agtgtaatta aaaatgtaaa agcgaaatta cattctttaa atattgatga
       61 tgttgaaata gttgaggttt atgatagaac ctctttaatt gatgctgcaa ttgatacttt
      121 aaaaagaact ttgattgaag agtctattat tgttatgtta atagttgcaa tattcttgtt
      181 tcattttaga agtgctttaa ttataattat tacccttcct ttaactgtaa tgattagttt
      241 tttacttatg aagctattta atattggctc aaatattatg agtttaggtg gaattgcaat
      301 tgcaattgga gctatggttg atgcaacaat tgtaatggtt gaaaatgctc ataaacatct
      361 tcaaggaaaa gaaaatataa caaaagagga gaggatagag ataatattat cttctgcaaa
      421 acaagttgga cgacctattt tctttgccct acttttggtt gtagtatcat ttttaccaat
      481 ttttgcacta agtggacaag aaggaaaact attttcacca cttgcattta caaaaacatt
      541 tgctatgctt tgtggggcga tgctttcaat taccctagtt cctattttga tgatatattt
      601 tataaaaggt aaaatcattc aagaggataa aaatatctta aatagttttt ttataaaaat
      661 ttattcacca atattaagat taagtttaaa attaaaatac ttggtggtta ctatattttt
      721 tataattata atttttgcct tatatacata ttcaaaacaa aaatgggaat ttatgcctac
      781 attaaatgag gcaacattta tgtatatgcc tgtaactcct tatggaattg gagctgattt
      841 agcaaagcag ttggcacaaa aaaccaatat ggttataaaa tcttttccag aagtgcaaaa
      901 tacttttgca aaagctggac gagcagcaac agcaactgat ccagcaccac ttgctatgat
      961 tgaaacaatt attactttta aaccacaatc acagtggaga gaaggaatga cttataaaaa
     1021 attaattgat gagatggata aaaaacttca aattccagga cttataaata catggacata
     1081 tccaattaaa ggtagaattg atatgttact tacaggaatt agaacacctt tgggaataaa
     1141 actttatggt aaagatttag aagttttaca agaagagagc tcaaaaatag agaacatatt
     1201 aaaaaaatat gaaggctcta tgagtgttag tgcagataag ataaatcaag gatattattt
     1261 aaatataaaa atagatgatg aagaaataag tagatataac ataagcaaaa acactatttt
     1321 agaaacttta agt
//
LOCUS       DAOQAN010000435         1330 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_160087_length_1330_cov_3.364706, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000435 DAOQAN010000000
VERSION     DAOQAN010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_160087_length_1330_cov_3.364706"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            124..>1330
                     /locus_tag="PKO10_06685"
     CDS             124..>1330
                     /locus_tag="PKO10_06685"
                     /inference="COORDINATES: protein motif:HMM:TIGR03648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VC_2705 family sodium/solute symporter"
                     /protein_id="HNW66871.1"
                     /translation="MELQSLIYLFVGVSFTIYFGLALWTKASSTKDFYVANKSFNPVF
                     NGASIAVDFISAATFISLCGAFLSFGYDSFAYIIGITSGFVLLTILIVPYLRKMECLS
                     IPLFFEKRFNSKLLKITTIIIVVLVSFIYISAQLKGVGIIFSRIFQLDLSTALLIGMC
                     ITLFYAFIGGMKNIAYTQIAQYIIILFSFMTPIFFLTIEFTNSYLPQLAIFSKSAIYE
                     ERYLIEVFEQTLNDYGFKSFSEFNVLNSLLIAISLSLGVASLPHILIKFFSTPTVNDA
                     RRSSFWALVFISVIYTSISSLFILSTLNLVKKTNSVEYDAFINNEIEGNEGKWLKTWE
                     KTGLVKFEDFNKNNKIDLKYENNISELSINPDALSLLTPEIANLPNWVISLVLAGALA
                     ATLSTITGLI"
BASE COUNT          453 a          161 c          171 g          545 t
ORIGIN      
        1 ttttgagtta aattatcata tttaatatct gaacaattta aaatttacct atttatttta
       61 ttaatattta tatatcaaaa acaggtttta aaattagaaa aaaaatatga gaacacctat
      121 taaatggaac ttcaatcttt aatatacctt tttgtaggtg tatcatttac aatatacttt
      181 ggtttagctc tttggacaaa agctagttct acaaaagatt tttatgttgc aaacaaatca
      241 tttaatccag tatttaatgg ggcttctatt gctgttgatt ttattagtgc tgcaacattt
      301 atatctttat gtggagcttt tttaagtttt ggatatgata gttttgctta tattattgga
      361 attacatctg gttttgtttt gcttacaatt ttaatagtac cttatctaag aaaaatggag
      421 tgtttatcta ttccactttt ttttgaaaaa agatttaact ctaagctact caagataact
      481 acaataataa ttgttgtttt ggtctcattt atatatattt cagcccaact aaaaggtgta
      541 ggaataatat tttcaagaat tttccaactt gatttatcaa ctgctctttt aataggaatg
      601 tgtataactc tattttatgc ttttattggt ggtatgaaaa atattgctta tactcaaatt
      661 gctcaatata ttattattct tttttcattt atgacaccta ttttcttctt aactattgag
      721 tttacgaact cttatcttcc tcaattagct attttttcta aatcagcaat atatgaagag
      781 agatacttaa ttgaagtttt tgaacaaact ttaaatgatt atggttttaa atctttttct
      841 gaatttaatg ttttaaatag tttattaata gctatatctt tgagtttagg agttgcttct
      901 ttaccacata ttttaatcaa atttttctca actcccacag taaatgacgc aagaagatca
      961 tcattttggg cattagtttt tatatctgtt atttatactt ctatatcatc tttatttatt
     1021 ctttctactt taaatttagt aaaaaagaca aatagtgtag agtatgatgc ttttataaat
     1081 aatgaaatag aaggaaatga aggtaaatgg ctaaaaactt gggaaaaaac tggtttagta
     1141 aagtttgaag attttaataa aaataataaa atagatttaa aatatgaaaa taatataagc
     1201 gaattatcaa taaatccaga tgcgctatcg cttttaactc cagaaattgc aaacttacca
     1261 aattgggtta tatctttagt tttagcaggc gcattagcag caacattatc tacaataact
     1321 ggtttgattt
//
LOCUS       DAOQAN010000436         1329 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_160264_length_1329_cov_3.337520, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000436 DAOQAN010000000
VERSION     DAOQAN010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1329
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_160264_length_1329_cov_3.337520"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            48..704
                     /locus_tag="PKO10_06690"
                     /pseudo
     CDS             48..704
                     /locus_tag="PKO10_06690"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
     gene            723..1199
                     /gene="folK"
                     /locus_tag="PKO10_06695"
     CDS             723..1199
                     /gene="folK"
                     /locus_tag="PKO10_06695"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HNW66872.1"
                     /translation="MKKKLSPNLTLFYTPNFPKIFNISSNKKYSVTIGVGGNIGDTKK
                     IFDKLILCLKKDSRFTLLKTSPLLKNPPFGFLEQSDFLNGIIRLQTDLCAFEFLQAMQ
                     RYEKKFGRKRSFQDAPRTLDIDIIFFDKKKINSKNLIIPHKNWANRESVIIPLKYT"
     gene            1222..>1329
                     /locus_tag="PKO10_06700"
     CDS             1222..>1329
                     /locus_tag="PKO10_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008339531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HNW66873.1"
                     /translation="MNKNKKIVVGMSGGVDSSVTALLLKQQGYDVVGLFM"
BASE COUNT          550 a          139 c          210 g          430 t
ORIGIN      
        1 accattcaga aagaaaatgt tgagatgata gccttcttaa aaaagcatca tttttaggaa
       61 aagatggttt taaagagttt gcaaaatata ttaaaaatag tggatttaaa aagagtgaaa
      121 aagagctata ttttaatgct tacaaattta tgagtaaaaa aggagagctt gaaacctctt
      181 ttaatccaat tgttgcaata aatgaaaacg cagcaaaacc tcacgcactt cctacaaata
      241 aaaaattaaa aaaagatgat ttgattttag ttgatgcagg agttaaatat aaaagatatt
      301 gttctgatag aacttgtact agtgttgctg attttgataa ctttaatttt gaaagaaagc
      361 aaacttttaa aaataaaaag catcaaaaaa tttatgacat tgtattaaaa gctcaagaaa
      421 aatctataaa agaggcaaga gctggaatga aagcttgtga tgttgataag attgctagag
      481 attttatctc tcaagctggt tatggaaaat attttgtgca tagcactggg catggtgttg
      541 gacttgatat tcatgaattt ccaaatataa atagcaaaaa tgagcttata attgaagata
      601 atatggtatt tacagtagaa cctggaattt atattccaaa tgagtttggt gtaagaattg
      661 aagatacagt ttatataaaa aatagtaaag ctgaaatttt ataaatagaa aaagagagaa
      721 atttgaaaaa aaaattatca cctaatttaa cactttttta tacaccaaat tttccaaaaa
      781 tttttaatat tagttcaaac aaaaaatata gtgtaactat tggagttggg ggaaatattg
      841 gagatacaaa aaaaatattt gataaattaa tcttatgttt aaaaaaagat tcaagattta
      901 ctttacttaa aacttcccct cttttaaaaa atcctccatt tggtttttta gaacaaagtg
      961 attttttaaa tggtataatt cgtcttcaaa cagatctttg tgcatttgag tttttacaag
     1021 ctatgcaaag atatgaaaaa aaatttggaa gaaagcgatc ctttcaagat gcgcctagaa
     1081 ccttagatat agatattata ttttttgata aaaaaaagat aaactcaaaa aatttaatta
     1141 tacctcacaa aaattgggca aatagagaat ctgtgattat tcctttaaaa tacacataaa
     1201 aaacttttta aaggtaaaaa aatgaataaa aataagaaaa tagttgtagg aatgtcaggt
     1261 ggagttgatt catctgttac agccttattg ttaaaacaac aaggatatga tgttgtcgga
     1321 ttgtttatg
//
LOCUS       DAOQAN010000437         1329 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_160320_length_1329_cov_2.345369, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000437 DAOQAN010000000
VERSION     DAOQAN010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1329
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_160320_length_1329_cov_2.345369"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            39..512
                     /locus_tag="PKO10_06705"
     CDS             39..512
                     /locus_tag="PKO10_06705"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNW66874.1"
                     /translation="MAIFDTSKGIVKIELKPKIAPKAVENFIGLAKKGYYDGQIFHRV
                     IKGFMIQGGDPTGTGAGGESIWNKSFEDEFAPNAVFDKPFILAMANRGKNTNGSQFFI
                     TTAPTYWLNGMHTIFGYVISGKDVVKDIENVKTNGRSGGDKPLEDVKINKITIEE"
     gene            complement(538..1014)
                     /locus_tag="PKO10_06710"
     CDS             complement(538..1014)
                     /locus_tag="PKO10_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNW66875.1"
                     /translation="MEHNHEKSATPNITEFMTFELGKMKYAIELPKIKEILTYPDNIT
                     ILPNTSDWVKGLINSRGEVVPILDIRIKFNTGPALYDVNTSIIIVITEDKRMVGIVVD
                     LVDDTQKIDTSMLASVSEMGSGIPARYLKGYVRVEDNKMLVIMDIEKVVCKEELED"
     gene            complement(1057..>1329)
                     /locus_tag="PKO10_06715"
     CDS             complement(1057..>1329)
                     /locus_tag="PKO10_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HNW66876.1"
                     /translation="VESQIKLLKEQFFQTLNQKFQIASNILESLKSNYLLNNPENRQK
                     DGLVELSKNKKLINLDLVKVDDIVELQSIDIIAECKIISLSKQKVK"
BASE COUNT          483 a          192 c          172 g          482 t
ORIGIN      
        1 tttttttatt tgcaaatgaa gatacaaaaa aagatcctat tgctattttt gatacatcta
       61 aaggtattgt aaaaatagaa ttaaaaccaa aaatagctcc aaaagctgtt gaaaatttta
      121 ttggtttagc aaaaaaaggc tactatgatg ggcaaatttt tcacagagtt ataaaaggat
      181 ttatgattca aggtggtgat cctactggaa ctggtgctgg tggagagtct atttggaaca
      241 aaagttttga agatgagttt gcaccaaatg cagtttttga taaaccattt attttagcta
      301 tggcaaatag aggtaaaaat acaaatggaa gtcaattttt tataacaaca gctccaacat
      361 actggttaaa tggaatgcat acaatttttg gatatgtaat tagtggtaaa gatgttgtaa
      421 aagatattga aaatgtaaaa acaaatggaa gaagtggtgg tgataaacct ttagaagatg
      481 ttaaaataaa taaaattaca atagaagagt aaaaaataaa aatagaggat tataaaatta
      541 atcctctagc tcttctttac aaacaacttt ttcgatatcc ataataacta gcattttatt
      601 atcttctact cttacataac cttttaaata cctagcagga attccagaac ccatctcaga
      661 aactgaagct aacattgaag tatctatttt ttgtgtatca tcaactaaat caactacaat
      721 tccaaccatt cttttatctt ctgtaattac tataataata gaagtattaa catcataaag
      781 agctggacct gtattaaact taattctaat atctaaaata ggaacaactt ctcctcttga
      841 attaatcaaa ccctttaccc aatcagaagt atttggtaaa atagtaatat tatctggata
      901 agtcaatatc tcttttattt ttggtaactc aatagcatat ttcattttcc ccaattcgaa
      961 tgtcataaac tcagttatat ttggagtagc gcttttttca tgattatgtt ccattttaat
     1021 ccccttattt caatattttc gtaagcaaaa ttgctgttat tttacctttt gtttacttaa
     1081 actaattatt ttacactctg caattatatc aatggattgt agctctacaa tatcatctac
     1141 ttttactaaa tccaaattta taagcttctt atttttactc aattcaacta aaccatcttt
     1201 ttgtctattt tctggattat ttaaaagata atttgatttt aaactctcca aaatattaga
     1261 agcaatttga aacttctgat ttaaagtttg aaaaaactgc tcttttaaaa gttttatttg
     1321 tgactctac
//
LOCUS       DAOQAN010000438         1328 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_160450_length_1328_cov_3.245090, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000438 DAOQAN010000000
VERSION     DAOQAN010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_160450_length_1328_cov_3.245090"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            71..544
                     /locus_tag="PKO10_06720"
     CDS             71..544
                     /locus_tag="PKO10_06720"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013135402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HNW66877.1"
                     /translation="MNEEIRREKSITMTMLMTPDKANFSGKNVHGGEILKMLDQVAYA
                     CAARYSGHYAVTLSVDMVLFKNPVKIGSLVTFHASVNYTGRTSMEIGIKVISEDIKDH
                     TMKNTNVCYFTMIAVDEDGKPVPVPKLELITEDDKRRYNDALKRRDFRMSSRHAK"
     gene            complement(572..>1328)
                     /locus_tag="PKO10_06725"
     CDS             complement(572..>1328)
                     /locus_tag="PKO10_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNW66878.1"
                     /translation="AKQATSRQKQLDKLDIQAIEVSSRRDPSIIFRQKREVGKELLTV
                     KNISKSYGDKVVLKDISFTLEKGDKIALIGPNGVGKTTLCEILVCNLQADSGEVLWGA
                     TIQNSYFPQNTTDIINGDMTLYDWLRGFDRDADIGEIRNCLGRMLFNGQEQEKKVDSC
                     SGGEKHRMMLSKIMLEQGNFLLLDEPTNHLDLEAIIALGEGLHQYAGSVICVSHDREL
                     LDAYANRIIEIQNDGTIIDFKGSYEEFMESKSA"
BASE COUNT          471 a          216 c          180 g          461 t
ORIGIN      
        1 ataaaataaa actgttgaat aagattaatt caataaaata caatatcaaa aatattttaa
       61 ggtataaaag atgaatgaag agataagaag agaaaaatct attacaatga ctatgctaat
      121 gactccagat aaagcaaatt tttctggaaa aaatgtacat ggtggtgaaa tattaaaaat
      181 gcttgatcaa gtagcttatg cttgtgcagc tagatattct ggtcactatg ctgttacgct
      241 ttctgttgat atggtgctat ttaaaaatcc agttaaaatt ggctctttgg taacttttca
      301 tgcttcagta aactacacag gacgaacatc tatggaaata ggtataaaag ttatatcaga
      361 agatataaaa gatcacacta tgaaaaatac aaatgtttgc tattttacaa tgattgcagt
      421 tgatgaagat ggaaaacctg tacctgttcc aaaattagaa cttataacag aagatgataa
      481 aagaagatat aatgatgctt taaaaagaag agattttaga atgtcatcaa gacatgcaaa
      541 atgataaaaa aggctaaaaa agcctttttt attaagctga tttagactcc ataaactctt
      601 cataacttcc tttaaagtca ataatagttc catcattttg aatttcaata attctatttg
      661 cataagcatc aagtaactct ctatcatgag atacacaaat aactgaacca gcatattgat
      721 gtaacccctc tcctaaagca ataatagctt caaggtcaag gtggtttgta ggctcatcta
      781 gtaaaaggaa atttccttgt tctaacataa tttttgatag catcattcta tgtttttctc
      841 ccccactaca agaatctact tttttctctt gctcttgacc gttaaataac attcttccaa
      901 gacaatttct aatctctcca atatctgcat ctctatcaaa acctctcaac caatcatata
      961 aagtcatatc accatttata atatcagttg tattttgtgg gaaataagag ttttgaatag
     1021 ttgctcccca taaaacctct ccactatcag cttgtaaatt acatactaat atttcacaaa
     1081 gtgttgtttt tccaactcca tttggtccaa ttaatgcaat tttatcacct ttttcaagag
     1141 taaatgaaat atcttttaat actactttat ctccataaga ttttgagata tttttaacag
     1201 ttaaaagctc ttttccaacc tctctttttt gtctaaatat aatagatgga tctcttctgc
     1261 ttgatacttc aatagcttga atatcgagtt tatcaagttg tttttgtctt gaagttgctt
     1321 gttttgct
//
LOCUS       DAOQAN010000439         1326 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_160865_length_1326_cov_2.851298, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000439 DAOQAN010000000
VERSION     DAOQAN010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1326
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_160865_length_1326_cov_2.851298"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..766)
                     /gene="surE"
                     /locus_tag="PKO10_06730"
     CDS             complement(<1..766)
                     /gene="surE"
                     /locus_tag="PKO10_06730"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HNW66879.1"
                     /translation="MMKILLTNDDGYDAVGLKALIKALTPIAKVMVVAPAKNKSACGH
                     SLTLDKPLRLISVDDEYYKIDDATPTDCIYLSLGHLFKNGYRPDLVISGINIGANMGE
                     DITYSGTASAAMEAVIHGVPAIAISQVCKDMCRSIVVDWDFELACKVVLELVSKIKNG
                     SFPLAERKFLNVNIPPLKVKDCKGVKITKAGYREYGNDAHRHTNPRGEEYYWIGLHPL
                     RWKPSLDSSCDFEAIKNGYVSISPIMLDMTSHSDIKN"
     gene            complement(741..>1326)
                     /locus_tag="PKO10_06735"
     CDS             complement(741..>1326)
                     /locus_tag="PKO10_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014475448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNW66880.1"
                     /translation="KDKIEKINYTLSSQIDEIADGSQILIVGKIETISEKISKKGSKF
                     GIVSILDFHGTVEFMLFEDKLKELQENFDLNEPIAFKVRISKNDQFTRMNVLKIETIF
                     DAQKEKIKIKAKEIEEPPLTVALPFSEDENSIYSLFDLIINNQGKRELKIIIKSKLAD
                     LELESGIKVSNAVEKLIHNIKGAYIIDDENSINK"
BASE COUNT          401 a          233 c          163 g          529 t
ORIGIN      
        1 aatttttgat atcgctatga gatgtcatat ctagcattat tggactgatt gatacatagc
       61 cattttttat agcttcaaag tcacaagaac tatcaagact aggtttccat cttaatggat
      121 gaagtcctat ccaataatac tcttcacctc ttggatttgt atgtctatgt gcatcatttc
      181 catactctct atatccagct tttgtgattt ttacaccttt gcaatctttt acttttaaag
      241 gtggaatatt aacatttaaa aactttcttt cagctagtgg gaaagagcca ttttttattt
      301 tagaaacaag ctctaaaact accttacaag ctagttcaaa atcccaatca acaacaatac
      361 ttctgcacat atctttacaa acttgagata tggcaattgc tggaactcca tgaattacag
      421 cctccatagc agcacttgct gttccactat atgtgatatc ttctcccata tttgcaccaa
      481 tatttatacc acttattact aaatcaggtc ggtaaccatt tttaaaaagg tgacctaaag
      541 ataagtatat acaatctgtt ggtgttgcat catctatttt atagtattca tcatcaacgc
      601 tgataagtcg taatggctta tctaaagtta aagagtgacc acatgcagat ttattttttg
      661 caggtgctac taccataact tttgctattg gtgttaaagc ttttattaaa gctttaagtc
      721 ctacagcatc atatccatca tcatttgtta atagaatttt catcatctat tatatatgct
      781 ccttttatat tgtgaattag tttttcaaca gcatttgata cttttatacc actttcaagc
      841 tctaggtctg ctaatttgga ttttattatt atctttagct ctctttttcc ttgattattt
      901 attattaaat caaataaact ataaatagag ttttcatctt ctgaaaatgg aagagctaca
      961 gtcaaaggtg gctcttctat ctcttttgcc ttaattttta ttttctcttt ttgggcatca
     1021 aaaatagtct ctatttttaa aacattcatt cttgtaaatt ggtcattttt tgagattcta
     1081 actttaaaag ctattggttc atttaaatca aaattctctt gaagttcttt taatttatct
     1141 tcaaaaagca taaattcaac agttccatga aaatccaaaa ttgatacaat tccaaattta
     1201 gaaccttttt ttgatatttt ctcagatatt gtttcaattt ttccaacaat taaaatctgg
     1261 ctaccatctg ctatttcatc tatttgtgaa gataaagtgt agtttatctt ttctatttta
     1321 tctttg
//
LOCUS       DAOQAN010000440         1325 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_161089_length_1325_cov_2.566929, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000440 DAOQAN010000000
VERSION     DAOQAN010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_161089_length_1325_cov_2.566929"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="PKO10_06740"
     CDS             complement(<1..288)
                     /locus_tag="PKO10_06740"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNW66881.1"
                     /translation="MKKEEILNKLKEIKPLYEKEGLEILGVFGSYAKDTQNEFSDIDI
                     AYRLDYNKFSQKYVDGFSKLLRIEEMKDELKKIFKKNIDFVPDKNKKILQGI"
     gene            complement(354..1070)
                     /locus_tag="PKO10_06745"
     CDS             complement(354..1070)
                     /locus_tag="PKO10_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66882.1"
                     /translation="MEIGSITNSYQTLEAKSNSQKDYGSSFSSFAELMRVNSVSSINQ
                     TKETKSINTSSNIEKTQKDYSGYTTFKELKEIPYEEAKENYEAIEKRALELDDKHRSE
                     GIEEGTAGLASRAMFEQLHKVNISNNDELNKALYETLRGIKDPLDSHVMNSELQFNLE
                     NYYHGNETRASFVYDPKTYGKNYELTKEQLSSINVDDFFSKMLNTFTKDYEAAPREAK
                     EQYKKIVDGYSIFSQVYNKL"
     gene            complement(1098..>1325)
                     /locus_tag="PKO10_06750"
     CDS             complement(1098..>1325)
                     /locus_tag="PKO10_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66883.1"
                     /translation="KYKDFENPERLMADYNNWQINDYEGFIKNLKNEYKLLSEDTIIS
                     SESRVLYKRYSQYMSDLETNYNKVINEPKYA"
BASE COUNT          386 a          213 c          125 g          601 t
ORIGIN      
        1 tattccttgt aagattttct tatttttatc tggaacaaaa tctatatttt ttttaaatat
       61 ttttttaagt tcatctttca tctcttctat tcttaaaagt tttgaaaaac catcaacata
      121 tttttgtgaa aatttattat aatcaagtct atatgcaata tctatgtcac taaactcatt
      181 ttgagtatct tttgcataac ttccaaaaac tcctaaaatt tctaaaccct ctttctcata
      241 aagtggtttt atctctttta atttatttaa aatctcttct tttttcatac taaactcttt
      301 ttgtacaaat ttcatttgta gattttataa catttggtat aaattttgac tttttagagt
      361 ttattataaa cctgcgaaaa tatgctatat ccatctacaa tttttttata ttgctctttt
      421 gcctctcttg gagcagcttc ataatctttt gtaaatgtat ttaacatttt agagaaaaaa
      481 tcatcaacat ttatgctact taactgttct tttgtcaatt catagttttt tccataagtt
      541 tttggatcat atacaaaact tgctcttgtt tcatttccat gatagtaatt ttcaaggtta
      601 aattgtaatt cagagttcat aacatgagaa tctaaagggt ctttgattcc ccttaaagtt
      661 tcataaagag ctttatttaa ttcatcatta tttgatatat taactttatg aagttgttcg
      721 aacatagccc tagaagctaa tcctgctgtt ccttcctcaa taccttcact tctatgctta
      781 tcgtctaact ccaatgctct tttctctata gcttcataat tctcttttgc ttcttcataa
      841 ggtatctctt ttaactcttt aaaagtagta tatccactat aatctttttg agttttttca
      901 atatttgaac tagtatttat actttttgtt tctttagttt gatttatact agatactgaa
      961 tttactctca taagctctgc aaaactgcta aaagaacttc cataatcttt ttgactatta
     1021 cttttagctt ctaacgtttg atatgaattt gttattgacc ctatttccat tttttatttc
     1081 cttatttatt tttaaatcta agcatactta ggttcattta ttactttatt atagtttgtt
     1141 tccaaatcac tcatatattg agaatatctt ttatataaaa ctcttgattc tgatgagatg
     1201 attgtatctt ctgagagtaa tttatactca ttctttaagt tttttataaa tccttcataa
     1261 tcatttattt gccaattatt ataatcagcc ataagtctct caggattttc aaaatcttta
     1321 tattt
//
LOCUS       DAOQAN010000441         1324 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_161289_length_1324_cov_2.394011, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000441 DAOQAN010000000
VERSION     DAOQAN010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_161289_length_1324_cov_2.394011"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..780)
                     /locus_tag="PKO10_06755"
     CDS             complement(<1..780)
                     /locus_tag="PKO10_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008341480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNW66884.1"
                     /translation="MEKIDDIKAYLKEHENKQLLRFITCGNVDDGKSTLIGRLLHDSK
                     GIFEDQLENIKKDSKKNNSTDNEFDLSLLVDGLQSEREQGITIDVAYRYFTTPKRKFI
                     IADTPGHEQYTRNMATGASTANLAIILIDARYGVQTQTKRHSFINKLLGIKHIVVAVN
                     KMDLMDFREDVFNKIKEDYLKFAKELGLSSNITLIPLSALNGDNVVERSSKSPWYNGE
                     TLIEHLENVQIDSDRDLTHFRFPVQYVNRANLDFRGFCGTVA"
     gene            complement(782..>1324)
                     /gene="cysD"
                     /locus_tag="PKO10_06760"
     CDS             complement(782..>1324)
                     /gene="cysD"
                     /locus_tag="PKO10_06760"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012148033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HNW66885.1"
                     /translation="LNKYKFDAVFGGARRDEEKSRAKERIYSFRDKNHRWGPKNQRPE
                     LWNIYNARVHKGESIRVFPLSNWTELDIWQYIYLEQIPIVPLYFAAKRPVVEKDGVKI
                     MVDDERMPIGPDDVIKEEMVRFRTLGCYPLTGAVNSTATTLEEIIKEMLLSRTSERQG
                     RVIDNDSAGSMEKKKIEGYF"
BASE COUNT          403 a          260 c          144 g          517 t
ORIGIN      
        1 agcaactgtt ccacaaaaac ctctaaaatc aagattagct ctatttacat attgaactgg
       61 aaatctaaag tgtgttaaat ctctatcact atcaatttgt acattttcta aatgttctat
      121 taatgtttca ccattatacc aaggagattt agagcttctt tcaactacat tatctccatt
      181 taaagcagat aatggaatta aagttatatt actagaaaga cctaactctt ttgcaaattt
      241 aagataatcc tctttaattt tattaaaaac atcttctcta aaatccatta agtccatttt
      301 attaacagct acaactatat gtttaattcc taataactta tttataaaag agtgtctttt
      361 cgtttgagtt tgaactccat atctagcatc tattaaaata attgctaagt ttgctgtact
      421 agctcccgtt gccatatttc ttgtatattg ctcatgacct ggagtatctg caataataaa
      481 ctttcttttt ggagttgtaa agtatctata tgcaacatct atagtgatac cttgctctct
      541 ctcactttgt aaaccatcaa ctaaaagtga taaatcaaac tcattgtctg tactattatt
      601 ttttttacta tcttttttta tattttcaag ctgatcttca aaaatccctt tactatcatg
      661 aagtaatctt ccaattaaag tagattttcc atcatcaaca tttccacaag ttataaatct
      721 aagaagttgt ttattctcat gctcttttaa ataagctttt atatcatcaa ttttttccat
      781 tttaaaaata cccttctatt tttttcttct ccatagaccc agcactatca ttgtctataa
      841 ctcttccttg tctctcactt gtacggctta gaagcatctc tttgattatc tcttcaagag
      901 ttgttgcagt tgaatttaca gcacctgtta aaggataaca tcctaaagtt ctgaatctaa
      961 ccatctcttc ttttattaca tcatctggac ctattggcat tctttcatca tcaaccatga
     1021 tttttacacc atctttttca acaactggtc ttttagccgc aaaatataat ggaactattg
     1081 gaatttgctc caaatagata tattgccaaa tatcaagctc cgtccagttt gaaagtggga
     1141 aaactctaat actttcacct ttgtgaactc ttgcattgta gatattccaa agttctggtc
     1201 tttgattttt tggaccccat ctatgatttt tatctctaaa agagtagatt ctctcttttg
     1261 cacgagattt ttcttcatcc cttctagctc ctccaaaaac agcatcaaac ttatatttat
     1321 ttaa
//
LOCUS       DAOQAN010000442         1323 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_161499_length_1323_cov_2.058360, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000442 DAOQAN010000000
VERSION     DAOQAN010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_161499_length_1323_cov_2.058360"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /gene="carB"
                     /locus_tag="PKO10_06765"
     CDS             complement(<1..640)
                     /gene="carB"
                     /locus_tag="PKO10_06765"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012013034.1"
                     /note="four CarB-CarA dimers form the carbamoyl phosphate
                     synthetase holoenzyme that catalyzes the production of
                     carbamoyl phosphate; CarB is responsible for the
                     amidotransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HNW66886.1"
                     /translation="MPKREDIKNILLIGSGPIVIGQACEFDYSGTQATKTLKELGYRV
                     VLVNSNPATIMTDPEFADKTYIEPITEEVVLNIIKKENIDAILPTMGGQTALNIATSM
                     YSKGLLEGITFLGVHPEAIKKGEDRHLFTESMKKIGLDLPRSENAYTLEEAMKLAKDI
                     GFPVISRASFTLAGAGSGVAYNMEEFKALAQAGLDASPINEIEILESVLGWKE"
     gene            complement(731..937)
                     /gene="xseB"
                     /locus_tag="PKO10_06770"
     CDS             complement(731..937)
                     /gene="xseB"
                     /locus_tag="PKO10_06770"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HNW66887.1"
                     /translation="MEDIKKQEDINFEDKITKAKELLEKLSNPQITINESLKLYNEGL
                     KELELAQKLLEEAKLVFVTQSNNS"
     gene            complement(940..>1323)
                     /gene="metX"
                     /locus_tag="PKO10_06775"
     CDS             complement(940..>1323)
                     /gene="metX"
                     /locus_tag="PKO10_06775"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509608.1"
                     /note="Catalyzes the conversion of acetyl-CoA and
                     L-homoserine to CoA and O-acetyl-L-homoserine; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HNW66888.1"
                     /translation="ETDGLYELFGRFEIEKYLEHNSYSFPKFFDPLSYLYICKTINIF
                     DAGRNKDKLEDSFLKIEGNLHLIAFKDDMLFFPNEMEEIRDIMIKIGKKDQVTFKLVD
                     SSSGHDSFLVEVEKFEEHIKDILKD"
BASE COUNT          412 a          240 c          150 g          521 t
ORIGIN      
        1 actctttcca acctaaaaca gactctaaaa tctcaatttc attgattggt gaagcatcaa
       61 gtcctgcttg agctagtgct ttaaactctt ccatattata agcaacacca cttccagcac
      121 ctgcaagtgt aaatgaagct cttgagataa ctggaaaacc aatatctttt gcaagcttca
      181 tagcctcttc aagagtataa gcattttcac ttcttggtaa atctaatccg atttttttca
      241 tactttcagt aaaaagatgt ctatcttcac cttttttgat agcttctgga tgaactccta
      301 aaaaagtaat tccttctaaa agtccttttg aatacattga agtcgctatg tttagtgcag
      361 tttgtccacc cattgtaggc aaaatagcat ctatattctc ttttttaatt atatttaaaa
      421 caacctcttc tgtaattggt tctatataag ttttatcagc aaattctgga tctgtcatga
      481 tagttgctgg gtttgagtta acaaggacaa ctctatatcc tagctctttc aaagtttttg
      541 ttgcttgagt accagaataa tcgaactcac acgcctgtcc tataacaatc ggtcctgaac
      601 caatcaacaa aatgtttttt atatcttctc tttttggcat aactttcctt tcactgattt
      661 aaaaatttgg caagtttacc caaaaagtac ttaatgtttt gttataaaag cactatcata
      721 taaagtttta tcaagagtta ttgctttgag taacaaaaac taattttgct tcttctaata
      781 acttttgagc tagctctaac tcttttaaac cttcgttata taactttaat gactcattta
      841 ttgtaatttg tggatttgaa agtttttcta aaagctcttt tgcttttgta attttatctt
      901 caaaattaat atcttcttgt tttttaatat cttccatttt taatccttta aaatatcttt
      961 tatatgttct tcaaattttt caacttctac taaaaaagag tcatgaccac tagaactatc
     1021 tactaattta aatgtcactt gatctttttt acctattttt atcataatat ctctaatctc
     1081 ttccatctca tttgggaaaa atagcatatc atctttaaaa gcaattaaat gaaggtttcc
     1141 ctctattttt aaaaatgaat cctctaattt atctttattt cttcccgcat caaaaatatt
     1201 tattgtttta caaatatata aatatgatag tggatcaaaa aattttggaa aactatatga
     1261 gttgtgctca agatatttct ctatctcaaa tcttccaaat agctcatata atccatctgt
     1321 ttc
//
LOCUS       DAOQAN010000443         1320 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_162019_length_1320_cov_2.364427, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000443 DAOQAN010000000
VERSION     DAOQAN010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1320
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_162019_length_1320_cov_2.364427"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(106..987)
                     /gene="glyQ"
                     /locus_tag="PKO10_06780"
     CDS             complement(106..987)
                     /gene="glyQ"
                     /locus_tag="PKO10_06780"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011373471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HNW66889.1"
                     /translation="MLTFSQMLLKLQEFWAKEGCNIVQPYDIPAGAGTFHPATLLRSL
                     DSTPWSVAYVAPSRRPTDGRYGENPNRLGAYYQFQVLIKPSLENIQDLYLKSLEYLGL
                     DLSRHDIRFVEDNWESPTLGAWGLGWEVWLDGMEVTQFTYFQQVGGLPCDPVAVEITY
                     GTERLAMYLQGVDSVFDIVWNENKHGITTYADVHKEGEYQFSKYNFEVANTTKLFRDF
                     EEAFNECKSCLEAQLPLPAYDQCMIASHAFNTLDARKAISVTERQNYILKVRELAQAC
                     AVMYKEQEKMRQERVRG"
     gene            complement(989..1219)
                     /locus_tag="PKO10_06785"
     CDS             complement(989..1219)
                     /locus_tag="PKO10_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014475118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HNW66890.1"
                     /translation="MKPIALFILPNCKWCKEAIIYLKSRKLKFNQIDLTKNKKALYDC
                     QKRCQGAPVFLVGDVWICGFDKEKLNKELGIK"
     gene            complement(1220..>1320)
                     /locus_tag="PKO10_06790"
     CDS             complement(1220..>1320)
                     /locus_tag="PKO10_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002869827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HNW66891.1"
                     /translation="ISDKDLAIKLKEDRIVKAKAVESDSKDIEVIL"
BASE COUNT          398 a          269 c          171 g          482 t
ORIGIN      
        1 aatctagctc atttcattcg ctacgattga tacacttcgt tagttcgcac cagccttttg
       61 tgaaagttct taactttcta caaaaggcaa aaaatgctaa aagagttagc ccctaactct
      121 ttcttgacgc attttttcct gctctttata cataacagca caagcttgtg caagttctct
      181 cacctttaaa atatagtttt gtctctctgt aacacttatt gcttttcttg catctaaagt
      241 attaaaagcg tgacttgcta tcatacattg atcataggct ggaagaggaa gttgtgcttc
      301 taaacaagat ttgcactcat taaatgcctc ttcaaaatct ctaaatagtt ttgttgtatt
      361 tgctacttca aaattatatt ttgaaaattg atactctccc tctttatgaa catctgcata
      421 agttgtaatt ccatgcttat tttcattcca aacaatatca aaaacagaat caactccttg
      481 aagatacata gcaagtctct ctgttccata agtaatctca acggcaactg gatcacaagg
      541 aagtcctcca acttgttgaa aatatgtaaa ttgagtaact tccataccat caagccaaac
      601 ttcccatcca agtccccatg ctcctagagt tggtgattcc cagttatctt ctacaaatct
      661 aatatcgtgc ctgcttaaat caagtcctaa atactctaaa gattttaaat ataaatcttg
      721 aatattttct aagcttggtt ttattaatac ttgaaattga tagtatgcac ctaatctatt
      781 tggattttca ccatatcttc catctgttgg tcttcttgat ggtgcaacat aagcaacact
      841 ccaaggagtt gaatcaagac ttctaagcaa agttgctgga tgaaatgttc ctgctcctgc
      901 tggtatatca tacggttgta caatattaca cccctctttt gcccagaact cttgaagctt
      961 taataacatt tgtgaaaaag taagcatctt attttattcc taactcttta tttaatttct
     1021 ctttgtcaaa accacatatc caaacatctc caactaaaaa aactggagca ccttgacatc
     1081 tcttttgaca atcatataag gcttttttat tttttgttaa gtctatttga ttaaatttca
     1141 gttttcgact ttttaagtag ataatagcct ctttgcacca tttacagttt ggtaaaataa
     1201 acagtgcaat tggcttcaat tataaaatca cttcaatatc ttttgaatca ctctcaacgg
     1261 cttttgcttt tacaattctg tcttctttta gttttattgc taaatcttta tcgctaattg
//
LOCUS       DAOQAN010000444         1317 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_162599_length_1317_cov_2.322504, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000444 DAOQAN010000000
VERSION     DAOQAN010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1317
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_162599_length_1317_cov_2.322504"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..238
                     /locus_tag="PKO10_06795"
                     /pseudo
     CDS             1..238
                     /locus_tag="PKO10_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011368646.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
     gene            579..857
                     /locus_tag="PKO10_06800"
     CDS             579..857
                     /locus_tag="PKO10_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014769437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNW66892.1"
                     /translation="MNKSEFIDAVAEKSGLTKKDSKIAVDAVLDTLTEALAKKDSVAF
                     LGFGTFATASREARTARVPGTDKTVNVPATTVAKFKVGKALKEAVANA"
     gene            complement(1002..>1317)
                     /locus_tag="PKO10_06805"
     CDS             complement(1002..>1317)
                     /locus_tag="PKO10_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HNW66893.1"
                     /translation="TTFYFKIRYEYGIKNSQSITENRLFNEKFLIDLAKFMIKKLDTR
                     LNYKIHYIAISASNFASDHNIKTFCLIDYKKDLKYKALNENLLKVRDKYGIDIVKYAS
                     EA"
BASE COUNT          529 a          163 c          163 g          462 t
ORIGIN      
        1 aaaagaagtt cttggaacaa ctatatctat tccaggaagt ccactaaaac gagatagtga
       61 ataccaaaag caaaacaaaa gagagaaatt caaaagaaga gccgccatag aaccaataat
      121 tggtcattta aaatcagatt tcagattagc aaaaaattat ctcaaaggat ttataggaga
      181 tcaaataaat ttacttctag cagcaactgc ttggaatcta aaaaagtgga taaatatcta
      241 tttaaaataa aatttaagtt cacatatact ttgctaatca aactatccag caatgtataa
      301 tttgcatttc aaaatcaaat tagctacaat caaaaagctt gagttagaat ataactacaa
      361 cccctattgc agaaaaatct agctcaagtg ctttaaaaat tataatgtat caaattcaat
      421 aaatttattc ccaatatttt ttataattca aaaaatttat aaaaaaatta cttaaatgcc
      481 ataaaataag ctttatatga atttttttgt agtaaatatt aaaatttcta taaaaattta
      541 gataaaatcg gaatcaaaat ttaacaagga gattttaaat gaataaaagt gaatttattg
      601 atgcagtagc agaaaaaagt ggtttaacaa aaaaagattc taaaatagca gttgatgctg
      661 tattagatac tttaacagaa gcattagcaa aaaaagattc agttgctttt ctaggatttg
      721 gaacatttgc aacagcttca agagaagcta gaacagctag agttccagga actgataaaa
      781 ctgttaatgt accagctaca actgttgcta aatttaaagt gggaaaagct ttaaaagaag
      841 ctgttgctaa cgcttaagta aaaatttata gatataaata tctaaaaact ttagacttct
      901 aaattatatt ggggtatata ttactctgat ataatcttag taattattga atacttttat
      961 acaattttga atattaccat taattaaaaa gtaatttcaa ctcatgcctc actagcatac
     1021 ttcacaatat caataccata tttatctctt acttttaata gattttcatt tagtgcttta
     1081 tattttagat cttttttata atctattaaa caaaaggttt taatattatg atctgatgca
     1141 aagtttgatg cacttattgc tatatagtgt attttatagt ttaatcttgt atctagtttt
     1201 tttatcatga attttgctaa atcaattaga aatttttcat taaatagtct attttctgta
     1261 attgattgag agttttttat tccatattca tatctaattt taaaataaaa tgttgtt
//
LOCUS       DAOQAN010000445         1317 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_162605_length_1317_cov_2.265452, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000445 DAOQAN010000000
VERSION     DAOQAN010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1317
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_162605_length_1317_cov_2.265452"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /locus_tag="PKO10_06810"
     CDS             complement(<1..743)
                     /locus_tag="PKO10_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66894.1"
                     /translation="MGKASFSVGKLTQHSQSHINRENGLNYAVVENSRANSFNKYYEY
                     DEFLQKAQDRYKEKVKQNMQNSAIVNIFQEAIIAIDGHHTSEDILDLFFELKQKYGGH
                     ELISLTIHKDEGYFTKNNQNFYPHKNILKKEDNNWYITQDGLNGKKPEDFSQKVDISE
                     FSTVLTPHAHAIFSMFDFSLGRNARMQKKDMMERLRFVATLLKMDYSLQKINKISNSD
                     TNLDLKDSEEIDTNSQMTIKNSYLIEINT"
     gene            complement(869..>1317)
                     /locus_tag="PKO10_06815"
     CDS             complement(869..>1317)
                     /locus_tag="PKO10_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014474282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HNW66895.1"
                     /translation="SKAFSKGGADLIGIGLTSIGNGVDYYAQNFKDLEPWENALDNGD
                     LPVFKGYRLSADDQLRQFVIMELMSNFSLNIKKVEEEFKIDFKDYFSDALESLKEFED
                     AELLKIYADRIEVSQTGTMLIRNICMPFDAYLNKIPEDKRRFSKTI"
BASE COUNT          426 a          199 c          146 g          546 t
ORIGIN      
        1 tgagtattta tctctattaa ataagagttt ttaattgtca tttggctatt tgtatcaatt
       61 tcttcactat cttttaaatc aagatttgta tcactatttg aaattttatt aattttttgt
      121 aaagagtaat ccatttttaa caaagttgct acaaacctta atctctccat catatctttt
      181 ttttgcattc ttgcatttct tccaagagag aagtcaaaca ttgaaaaaat agcgtgtgca
      241 tgaggagtta aaactgtaga aaattccgaa atatctactt tttgtgaaaa atcttctggt
      301 tttttaccat ttaaaccatc ttgagttata taccagttgt tatcttcttt ttttaatatg
      361 tttttgtgtg gataaaaatt ttgattattt tttgtaaaat agccttcatc tttatgaatt
      421 gtaagagata ttaactcgtg tccaccatat ttttgtttta attcaaaaaa tagatctaaa
      481 atatcttctg atgtatgatg tccatctatt gcaataatag cctcttgaaa tatattgaca
      541 atagcactat tttgcatatt ttgttttact ttttctttat atctatcttg agctttttgt
      601 aaaaattcat catattcata atatttatta aaactattag ctcttgaatt ttcaactaca
      661 gcatagttta aaccattttc tctatttatg tgcgattgtg agtgttgtgt taattttcct
      721 acactaaaag acgctttccc catattttcc cctatatgtt atggtgctat aagcaccatt
      781 gaaattgtgt agtaagatta aacttaattt ttattaaaag tttgcttaaa aaaatggaca
      841 aaaaagccca ttaatttatt aaaataattt aaattgtttt agaaaatctt cttttatctt
      901 ctggaatttt atttaagtaa gcatcaaaag gcatacatat atttctaatt aacatagttc
      961 ctgtttgaga aacttcaatt ctatcagcat atattttaag aagttctgca tcttcaaact
     1021 cttttaaaga ttctagtgca tcgctaaaat aatctttaaa atctatttta aactcctctt
     1081 caactttttt tatatttaaa gagaaattac tcataagttc cataattaca aattgtctta
     1141 attggtcatc agcacttaat ctataacctt tgaatactgg caaatctcca ttatccaaag
     1201 cattttccca aggttctaaa tctttgaaat tttgtgcata ataatcaaca ccatttccta
     1261 tagatgtaag tcctattcct attaaatcag ctccaccttt tgaaaatgct ttggata
//
LOCUS       DAOQAN010000446         1315 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_162941_length_1315_cov_2.514286, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000446 DAOQAN010000000
VERSION     DAOQAN010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1315
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_162941_length_1315_cov_2.514286"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="PKO10_06820"
     CDS             complement(<1..692)
                     /locus_tag="PKO10_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HNW66896.1"
                     /translation="MNKKTVGIIGVGNVGSNLAFCLATSNLCKSILLKDIREEFTQAM
                     ALDISQAAKSKNSATKVKACINNSDFKNCDIIVITAGIARKPNMSREDLIFTNKKILE
                     SIFDEVLENNKNAIFIIVSNPLDAMVYVALNKSKLPRNKVFGMAGVLDSARFKHYIEE
                     KLDFDYTNIEAMVIGAHSNTMVPLINHAKVDGKKVVDILKSEDIEYILENTKNGGARV
                     VDLLKTGSAYFAP"
     gene            complement(786..>1315)
                     /locus_tag="PKO10_06825"
     CDS             complement(786..>1315)
                     /locus_tag="PKO10_06825"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HNW66897.1"
                     /translation="KMLSIVPLMNGGGLFETGAGGSAPKHVQQLQEENYLRWDSLGEF
                     MALAASFDHLANTQNNKKAAVLAKTLDKATGTFLINDKSPARKVGSIDNRGSHFYLAM
                     YWADELAKQNDDAELKAEFTPIAKAMNENEAQIVKELTEVQGKPANTGGYYLFDDELT
                     SKVMRPSATLNKIIG"
BASE COUNT          418 a          225 c          163 g          509 t
ORIGIN      
        1 ggtgcaaaat atgcagaacc tgtttttaat aaatcaacaa ctctagctcc accattttta
       61 gtattttcta aaatatattc aatatcttct gattttaata tatcaacgac ttttttacca
      121 tcaacttttg catgatttat caaaggaacc attgtatttg aatgtgctcc tataaccata
      181 gcttctatat tggtgtaatc aaaatctaat ttttcttcta tatagtgttt aaatcttgca
      241 ctatctaaaa caccagccat accaaaaact ttatttctag gtaactttga cttatttaat
      301 gctacataaa ccattgcatc taaaggattt gaaactataa taaaaatagc atttttattg
      361 ttttctaaca cttcatcaaa aatagattct aatatttttt tatttgtaaa tattaaatct
      421 tctctactca tatttggttt tcttgcaatt ccagctgtga ttactataat atcacagttt
      481 ttaaaatcac tattatttat acaagcttta acttttgtag cgctattttt agattttgct
      541 gcttgagata tatctaaagc catagcttgt gtaaattctt ctctaatatc ttttagtaaa
      601 atagatttac aaagattgct tgtagctagg caaaaagcta agtttgaacc aacatttcca
      661 acaccaataa ttccaacagt ttttttattc acatcgagcc tttttaaatt tgaagagcaa
      721 attaagagtt ttcataagaa agattttaaa tttagactaa aaagaggaaa tcctcttttt
      781 agtagttatc caataatttt gtttaaagtt gcagaaggtc tcataacttt tgaagttaat
      841 tcatcatcaa ataggtagta accacctgta tttgctggtt ttccttgaac ttcagtaagt
      901 tcttttacaa tttgagcttc attttcattc attgcttttg caattggagt aaattcagct
      961 tttaattcag catcatcatt ctgttttgct aactcatctg cccagtacat tgcaagatag
     1021 aaatgacttc ctctattatc aatgcttcca acttttctag ctggtgattt gtcattaatt
     1081 aggaatgttc cagtagcttt atctaaagtt tttgctaaaa cagctgcttt tttattattt
     1141 tgagtatttg ctaaatggtc aaacgaagca gcaagagcca taaattcacc taaactatcc
     1201 catcttaaat agttctcttc ttgaagttgt tgaacatgtt taggagcaga tcctccagca
     1261 cctgtttcaa acagtcctcc accattcata agtggaacaa ttgataacat ttttg
//
LOCUS       DAOQAN010000447         1314 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_163168_length_1314_cov_2.455123, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000447 DAOQAN010000000
VERSION     DAOQAN010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_163168_length_1314_cov_2.455123"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="PKO10_06830"
     CDS             complement(<1..250)
                     /locus_tag="PKO10_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNW66898.1"
                     /translation="MEIKNLKLLVAPILGLLISLPILILIIYFLFNGSFNKEFLESSF
                     LLEYSLNTIYLIIGTAIFVIIFGVVTSYLSARFEYFGSK"
     gene            complement(241..1251)
                     /locus_tag="PKO10_06835"
     CDS             complement(241..1251)
                     /locus_tag="PKO10_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) ABC transporter substrate-binding
                     protein"
                     /protein_id="HNW66899.1"
                     /translation="MVKKLALCSLLFANILFASGSEVNVYSQRHYDSDKALFKKFEEK
                     TGIKVNLITAKAEELVSRLSIEGANSPSDILITADIGNLYEAKERDLLQGVSSKVLEK
                     NIPSHLRDEDGKWFAITKRARLFVYNPKTINPAHLDDYFSLTKPEFKGKVITRSSTNA
                     YNKSLLASIIAHNGEEKSLEFVKGLVNNFARDPKGADKDQIRAVASGEADIAIVNSYY
                     LGVMANSGDKVDEEIAKEVKVFFPAQKTTGAHINISGAGITKFAPNKENAIKLIEFLT
                     SEEAQGELAQGNYEYPVNPKVKPAGIVASWGEFKEDTIPLNEVGKQTRKAVEIATKGN
                     WK"
BASE COUNT          402 a          234 c          174 g          504 t
ORIGIN      
        1 acttactacc aaagtactca aatctagcac ttaagtagga agttactact ccaaatatta
       61 ttacaaatat tgcagtacct attattaaat atattgtatt taatgaatat tctaataaaa
      121 aactactctc taaaaactct ttattaaaag agccattgaa tagaaaatat attattagta
      181 ttaaaattgg tagtgatata agtagcccta agataggggc tactaaaagc tttagatttt
      241 ttatttccaa ttcccttttg tcgctatttc aacagctttt cgtgtctgtt ttccaacttc
      301 atttaaaggt attgtatcct ctttgaactc tccccaagaa gcaactattc cagctggttt
      361 aacttttgga ttaacaggat attcatagtt tccttgtgcc aactcacctt gtgcttcttc
      421 gcttgttaaa aactctatta gttttatagc attttcttta tttggagcaa attttgtaat
      481 accagcacct gagatattta tatgagcacc tgtagttttt tgtgcaggaa aaaatacttt
      541 tacctctttt gctatttcct catcaacttt atcaccacta tttgccataa ctcctaaata
      601 atatgagttt acaatagcaa tatcagcttc accagaagct actgctctta tttggtcttt
      661 atcagcacct tttggatctc ttgcaaagtt atttactaaa ccttttacga actctaaaga
      721 tttttcttca ccgttatgtg cgataataga agcaagaagt gacttgttgt aagcatttgt
      781 tgaacttcta gttatcactt ttcctttaaa ttcaggtttt gtaagtgaaa aatagtcatc
      841 taagtgtgct ggatttatag tttttggatt ataaacgaaa agtctagctc tttttgtaat
      901 agcaaaccat tttccatctt catctcttag gtgagaggga atattttttt ccaatacttt
      961 tgagctaact ccttgaagca aatctctctc ttttgcttca tataaatttc caatatcagc
     1021 tgttataaga atatctgaag ggctatttgc accttctatt gacagtcttg aaacaagctc
     1081 ttcagctttt gcagttataa ggtttacttt aatacctgtt ttttcctcaa attttttaaa
     1141 cagtgcctta tctgagtcat agtgtctttg tgaatataca tttacttcac taccacttgc
     1201 aaacaaaata tttgcaaata gtagtgagca aagagccaat tttttaacca tctaactatc
     1261 cttttttatt ttataatcca accggaatat ttgaaagaac aatttttata agaa
//
LOCUS       DAOQAN010000448         1314 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_163213_length_1314_cov_2.110405, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000448 DAOQAN010000000
VERSION     DAOQAN010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_163213_length_1314_cov_2.110405"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /gene="xseA"
                     /locus_tag="PKO10_06840"
     CDS             complement(<1..558)
                     /gene="xseA"
                     /locus_tag="PKO10_06840"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HNW66900.1"
                     /translation="MNPISVTTLNTQIKSLLETTFMQVFVSGEISNLVIHSSGHIYFS
                     IKDDNSTISCVMFKGNTKYLKFELENGQKVQITANITVFVPRGNYQLLCTKIEPDGIG
                     SLALAYEQLKTKLQAKGYFEQSIKKPLPKYPKKIAIVTSATGAAIEDMKKVASARWNL
                     VELILIPTLVQGNGSIEDIAKNIKFA"
     gene            complement(559..1269)
                     /gene="ubiE"
                     /locus_tag="PKO10_06845"
     CDS             complement(559..1269)
                     /gene="ubiE"
                     /locus_tag="PKO10_06845"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HNW66901.1"
                     /translation="MEKQEKIVSMFNNIAPTYDKANRVLSMGIDKSWRDKACNKTFEL
                     YGKKEIEKIVDVACGTGDMIIFWKQNANKKGITLKNIVGIDPSVGMMEVGKKKLPEVQ
                     FIEAFATSMPLENESVDIVSISYGIRNVVQREEAFVEFARVLKKGGLVVISEFTKNQK
                     ETPIDYLTAFYMDKILPVVGGIISKNKEAYRYLPDSIDEFLTTSNLQKELKNAGLEPI
                     YTKAFSMKISTLIIAKKI"
BASE COUNT          410 a          216 c          142 g          546 t
ORIGIN      
        1 agcaaatttt atattttttg caatatcttc aatacttcca tttccttgaa caagagttgg
       61 aataagtatt aactcaacta aattccatct agcacttgca acttttttca tatcttctat
      121 agcagcacct gtagcacttg ttacaattgc aatttttttt ggatattttg gaagaggctt
      181 ttttatactt tgttcaaaat aaccttttgc ttgaagtttt gttttaagtt gctcatatgc
      241 taaagccaaa cttcctattc catctggctc aattttagta caaagaagtt gataatttcc
      301 tcttggaaca aaaacagtta tatttgcagt tatttgtact ttttgtccat tttctaactc
      361 aaatttcaga tattttgtat tacctttaaa cattacacaa gatattgttg aattatcatc
      421 tttaattgaa aaatatatgt gcccagagct gtgaataact agatttgaaa tctctccaga
      481 aacaaaaact tgcataaaag ttgtctctaa aagagatttt atttgtgtat ttaaagtagt
      541 tacactaatt ggattcatct atattttttt tgcaattatt agtgtagaaa ttttcataga
      601 aaaggctttt gtgtatattg gttctaatcc agcattttta agctcttttt gtaaatttga
      661 tgttgttaaa aactcatcaa tagagtctgg taaatatcta taagcctctt tattttttga
      721 tattattccg ccaacaactg gtaaaatttt atccatataa aatgctgtta aataatcaat
      781 tggtgtctct ttttgatttt ttgtaaattc actaattaca actaaaccac cttttttaag
      841 aactcttgca aactctacaa aagcctcttc tctttgtact acatttctaa ttccataaga
      901 gattgatact atatcaacac tctcattttc aagtggcata gatgttgcaa aagcttctat
      961 aaattgaact tctggaagtt ttttctttcc aacttccatc ataccaacac ttggatctat
     1021 tccaacaata ttttttaggg taattccttt tttatttgca ttttgtttcc aaaaaattat
     1081 catatcacca gttccacaag caacatcaac aattttttct atctcttttt taccatataa
     1141 ttcaaaagtt ttattacaag ctttatctct ccaactttta tctattccca tagataaaac
     1201 tctatttgct ttatcataag ttggtgcaat attattaaac attgatacta ttttttcttg
     1261 tttttccata taaatttttc ctattttata aatgtttttt ctaataaatt ataa
//
LOCUS       DAOQAN010000449         1313 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_163313_length_1313_cov_3.408585, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000449 DAOQAN010000000
VERSION     DAOQAN010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_163313_length_1313_cov_3.408585"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1313
                     /locus_tag="PKO10_06850"
     CDS             <1..>1313
                     /locus_tag="PKO10_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fatty acid--CoA ligase family protein"
                     /protein_id="HNW66902.1"
                     /translation="IFALNQIFNNTSTTPNHYNSKSGEPAYLVYTSGTTGYPKGVLHS
                     HRSLIGRKPATDFWFDFKENDRIMHSGKFNWTYVLGSALMDPLFNGHTVIAYEGANDA
                     STWINLIKKHQCTIFIGVPTIYRQIIQKTDFTLDDCPSLRYCMSAGEHLSDEMLGLWR
                     DRFKQDIYEAIGMSECSYYISHSKNNPIRPGSAGFPQPGHIVKLLDPETLEEVGVEEE
                     GMICIGEDDPGLFLEYWQLEEETLKSRHDGYFFTGDYARKDKDGYIWFIGRKDDIINT
                     FGFRVSPHEIERVVKTHADVADCVAFGLDIEKEKTIVAIAVVGHSTISKEKEQEILKF
                     AQNNLAKYKAPKEIFIMSDYPRTKNGKVLRKQLVKDLHEQYFAITKGEEIVEYKARRS
                     MLLIPSYNKHNVEKAKTVLADTVIFDLEAILEDQREPGRETLKEV"
BASE COUNT          506 a          164 c          248 g          395 t
ORIGIN      
        1 aaatatttgc tctaaatcaa atattcaata atacaagtac aacaccaaat cactacaact
       61 caaaatcagg tgaaccagca tatttagttt atacatctgg aacaactggt tatccaaaag
      121 gtgttttaca ttcacataga tcactaatag gaagaaaacc agcaacagat ttctggtttg
      181 attttaaaga gaatgataga attatgcact ctgggaaatt taactggact tatgttttag
      241 ggtcagcact tatggatcca ttatttaatg gtcatacagt aattgcatat gagggtgcaa
      301 atgatgcatc aacatggatt aatttaataa aaaaacatca atgtacaata tttattggag
      361 ttccaacaat ctatagacaa attattcaga aaacagattt cacacttgat gattgcccaa
      421 gtttgagata ttgtatgagt gcgggagagc atttaagtga tgagatgctt ggactttgga
      481 gagatagatt taaacaagat atttatgaag ctattggaat gagtgagtgt tcatactata
      541 tttctcactc aaaaaataat cctataagac caggaagtgc aggtttccca caaccaggac
      601 atattgtaaa acttttagat cctgaaactt tagaagaagt tggagttgaa gaggaaggaa
      661 tgatttgtat tggagaagat gatccaggat tatttttaga gtattggcaa ttagaagaag
      721 agactttaaa atcaagacat gatggatact tctttacagg tgattatgca agaaaagata
      781 aagatggata tatctggttt attggtagaa aagatgatat tatcaatact tttggattta
      841 gagtaagtcc acatgagatt gaaagagttg taaaaactca cgcagatgta gctgattgtg
      901 ttgcttttgg tcttgatatt gaaaaagaga aaactattgt tgctattgct gttgttggtc
      961 attcaactat aagtaaagag aaagagcaag agattttaaa atttgctcaa aataatcttg
     1021 caaaatataa agctccaaaa gagattttta taatgtctga ttatccaaga acaaaaaatg
     1081 gaaaagttct tagaaaacaa ttagtaaaag atctacacga acaatatttt gcaataacaa
     1141 aaggtgaaga gatagttgag tacaaagcaa gaagatcaat gcttttaatt ccttcatata
     1201 ataaacacaa tgtagaaaaa gctaaaactg ttttagcaga tactgttatt tttgatttag
     1261 aagctatctt agaggaccaa agagaaccag gaagagagac tttgaaagaa gtt
//
LOCUS       DAOQAN010000450         1313 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_163375_length_1313_cov_2.368045, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000450 DAOQAN010000000
VERSION     DAOQAN010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_163375_length_1313_cov_2.368045"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1158
                     /gene="bioA"
                     /locus_tag="PKO10_06855"
     CDS             <1..1158
                     /gene="bioA"
                     /locus_tag="PKO10_06855"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HNW66903.1"
                     /translation="NGEILIDGMSSWWSAILGYNNKKIYKSLKKQAKIMPHIMFGGLT
                     HSPASILAKKLVEITGLNSVFLCDSGSVSVEVALKTAILYQKAKGKEKFKFLALKNAY
                     HGDTLGAMSVCDPENSMHSLYGDYLSSNIFVEAPELGFEADFSKALEDLEKTLKINHE
                     KIAGFILEPVVQGAGGMRIYNPKYLEKVRELCTKYDILMILDEIATGFGHTGEMFAFL
                     HTNIKPDILTIGKGLTGGTLTMAAMITSKDISDVISNSDIKVLMHGPTFMANPLACSV
                     ANAAIDELLNYDWRKKVLKIEKIFKKDLEKVKDSFLVNDIRNIGAIGVIELKDDIYAS
                     QLQDYCVKNGVWLRPFGKLFYSIVAYTISKKELKKITKTMIEAIKYLEELS"
BASE COUNT          496 a          161 c          228 g          428 t
ORIGIN      
        1 aacggtgaga ttttaattga tggaatgagc tcatggtgga gtgcaatttt aggttataac
       61 aataaaaaga tatataaatc tttgaaaaag caagcaaaaa ttatgcctca tattatgttt
      121 ggaggtttaa ctcattctcc agcttcaatc ctagccaaaa agcttgtaga aataactggt
      181 ttaaactctg tatttttatg tgatagtggc tctgtttcag tagaagttgc attaaaaaca
      241 gcaattttgt atcaaaaagc aaaaggcaaa gagaagttta aatttttagc tttaaaaaat
      301 gcttatcacg gagatacatt aggagctatg agtgtttgtg atcctgaaaa ttcaatgcac
      361 tctttatatg gtgactattt aagttctaat atttttgtag aggctccaga attgggattt
      421 gaagcagatt ttagcaaagc tttagaagat ttagagaaaa ctttaaaaat aaatcatgaa
      481 aaaattgcag gatttattct tgaaccagtt gtgcaaggtg caggtggaat gaggatatat
      541 aatcccaaat atttagaaaa agtaagagaa ctttgtacaa aatatgatat tttgatgatt
      601 ttagatgaga ttgcaactgg ttttggacat acaggagaga tgtttgcttt tttacataca
      661 aatataaaac cagatatttt aactattgga aaaggtctta ctggtggaac tttgacaatg
      721 gccgcaatga ttacatctaa agatataagt gatgttattt caaatagtga tattaaagtt
      781 ttaatgcatg gaccaacatt tatggcaaat ccactagcat gtagtgttgc aaatgcagct
      841 attgatgagc ttttaaatta tgattggaga aaaaaagttt taaaaattga gaaaattttt
      901 aaaaaagatt tagaaaaagt aaaagatagc tttttagtaa atgatataag aaatattgga
      961 gcaataggag taatagagct aaaagatgat atttatgctt cacaacttca agattattgt
     1021 gtaaaaaatg gagtttggct tcgtcctttt ggaaaacttt tttactctat cgttgcatat
     1081 acaatatcaa aaaaagagct aaaaaaaatc acaaaaacca tgatagaagc aataaaatat
     1141 ctagaagaac tctcttaaat agttaaaaca gaaaaagctt catcttttaa ggcgtttgtt
     1201 aaaaattcac cagtatgagt tatttcaata ccaagttttt ctaattctgc tcttgcacca
     1261 taatcttcta cacaaactat acaagcactc attgttacac ctgtgcttaa aat
//
LOCUS       DAOQAN010000451         1309 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_164107_length_1309_cov_3.688995, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000451 DAOQAN010000000
VERSION     DAOQAN010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_164107_length_1309_cov_3.688995"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="PKO10_06860"
     CDS             complement(<1..345)
                     /locus_tag="PKO10_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66904.1"
                     /translation="MYTKEEQLKKVKKKKVSTFGKKNILKKKSQNLSKDEKDFLNWLQ
                     TRENTVCFVCGKNDFYDKIEWHHIKRSSSDTKNHFRLIPLCGLKHQRNGELSPHGGAR
                     RFRETFTYEEQLF"
     gene            complement(542..772)
                     /locus_tag="PKO10_06865"
     CDS             complement(542..772)
                     /locus_tag="PKO10_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66905.1"
                     /translation="MLNDNIKRAIKAVNSVKDFDDLLYTFQMSDANQALKDEAIRELK
                     AVKSAFFINPLYTPKKVYEDIKASEADISDIN"
     gene            complement(777..935)
                     /locus_tag="PKO10_06870"
     CDS             complement(777..935)
                     /locus_tag="PKO10_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66906.1"
                     /translation="MIDLLKKIALFVFMVLLCFAFSWMLLYAADEEYHIKEQKEKAYL
                     ENIENSRS"
     gene            complement(932..>1309)
                     /locus_tag="PKO10_06875"
     CDS             complement(932..>1309)
                     /locus_tag="PKO10_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66907.1"
                     /translation="KEMVKMLIKKYNCNYIKVVPGLDISKAGSVSNLEAKIIFDIKND
                     KDGRHLLIKDTITKDNIYLYYEFLEKEDIKTSFQSCENCLYKHKKIYKDYDTIKCELD
                     NFSNLDLKNQIGDKFCNKWEYKK"
BASE COUNT          438 a          212 c          106 g          553 t
ORIGIN      
        1 aaacaactgc tcttcataag tgaaagtttc tctaaacctt ctagctcctc catgaggact
       61 taactctcca tttctctgat gttttaaacc acataaagga ataagtctaa aatgattttt
      121 tgtatcactt gaacttcttt ttatatgatg ccattcaatt ttatcataaa aatcattttt
      181 accacaaaca aagcaaactg tattttctct agtttgtagc caatttaaaa aatctttttc
      241 atctttagat aaattttgag attttttttt caaaatattt tttttcccaa atgttgaaac
      301 cttttttttc ttaacttttt tcaactgctc ttctttagtg tacataaaac tcctttttta
      361 aaatctagta aaaatccaaa aaaattggtg gggactccta ctatgccttt ttagtaagca
      421 taccaattta taaacgcttc ataatccata caaaaatctt ttttacctaa atttgatttt
      481 tgaatttttg aacctctttt ttttaaatat attttaaaaa catttttcat aatttacctt
      541 tttagtttat atcgcttata tctgcttcac ttgcttttat atcttcatat actttttttg
      601 gagtatataa gggatttata aaaaaagcac ttttgaccgc ttttaattct cttatagctt
      661 catcttttaa agcttgattt gcatcgctca tttgaaaagt atataaaaga tcatcaaaat
      721 ctttaacact atttacagct ttaatagctc ttttaatatt atcatttaac attttattaa
      781 ctccttgaat tttcaatatt ctctaaatag gctttttctt tttgctcctt aatatgatat
      841 tcttcatcag cagcatacaa aagcatccag gagaaagcaa aacataaaag caccataaaa
      901 acaaacaaag ctattttttt taataaatca atcatttttt atactcccat ttattacaaa
      961 atttatctcc aatttgattt tttaaatcaa gatttgaaaa attatccaac tcacatttta
     1021 tagtatcata atctttatat atttttttat gtttatataa acaattttcg caactttgaa
     1081 aagaagtttt tatatcttct ttttctaaaa actcataata taaatatata ttatcttttg
     1141 taatagtatc ttttataagc aagtgcctac cgtctttatc atttttaata tcaaatataa
     1201 ttttagcttc aagatttgaa actgaaccag cctttgaaat atctaaacca ggcacaactt
     1261 taatataatt acaattatat tttttaatta gcatttttac catttcttt
//
LOCUS       DAOQAN010000452         1308 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_164299_length_1308_cov_4.053472, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000452 DAOQAN010000000
VERSION     DAOQAN010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1308
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_164299_length_1308_cov_4.053472"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /locus_tag="PKO10_06880"
     CDS             complement(<1..674)
                     /locus_tag="PKO10_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasminogen-binding N-terminal domain-containing
                     protein"
                     /protein_id="HNW66908.1"
                     /translation="MKKMFTKLAFIGAILFATNSLAVETVCYKNGLDTPSQIETAKLE
                     GGICGGKTTVGEMLKNGWQILDINVTTENGKLSYSYYFYQNAKQGLSSGTYASKLDYG
                     KKEFSISPMAFKIDNLENNRTTIPKGNLLVGQSGIVVHIYDNDRRLIVSNAKVVSSNE
                     KSSVIEYFAFDDLKQDAIPNTNRVVEKGDILLLNYMYDQSLLIAPDFNNYKAVKDDFA
                     QNNFIHP"
     gene            complement(683..1192)
                     /gene="bcp"
                     /locus_tag="PKO10_06885"
     CDS             complement(683..1192)
                     /gene="bcp"
                     /locus_tag="PKO10_06885"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013136100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HNW66909.1"
                     /translation="MLKVGDKAPAFCAFNQDDTEICLRDIVGNWIVLYFYPKDMTPGC
                     TTQACDFTANLYKFDNLKATVIGVSPDDSAKHRKFIEKYDLAITLLADEDKKMAQDYG
                     VWQLKKFMGKEFMGVIRTTFIINPKGEIAYIWDKVSVRKTKSVKGEKIEILHVDEVRE
                     KLEELQSKY"
BASE COUNT          415 a          233 c          136 g          524 t
ORIGIN      
        1 ggatgtataa aattattttg tgcaaaatca tcttttactg ctttatagtt attaaaatca
       61 ggagctataa gtaaagattg gtcatacata tagtttaaaa gtaaaatatc acctttttca
      121 actactctat ttgtattagg aatcgcatct tgttttaaat catcaaaagc aaaatactca
      181 ataacagaac ttttttcatt tgagcttact acttttgcat ttgaaactat taatcttcta
      241 tcattgtcat aaatgtgtac aacaattcca ctttgaccta caagtagatt tccttttggt
      301 attgttgttc tattattttc taaattatca attttaaatg ccataggtga aatagagaac
      361 tctttttttc cataatccaa ttttgatgca taagttccac tacttaaacc ctgttttgca
      421 ttttgataaa agtagtatga ataacttaac tttccatttt cagttgttac atttatatct
      481 aaaatttgcc aaccattttt taacatttca ccaacagtag ttttaccacc acaaattcca
      541 ccttcaagct ttgcagtttc tatttgagat ggtgtatcta aaccattttt ataacaaaca
      601 gtttcaactg ctaaactatt tgtagcaaat aaaatagcac caataaaagc taactttgta
      661 aacatttttt tcatcttttt tcctaatatt ttgattgtaa ttcttctaat ttttctctaa
      721 cttcatccac atgtaaaatt tcgatttttt ctccttttac actctttgtt tttctcacac
      781 ttactttatc ccaaatatat gctatctctc cttttggatt tattataaaa gtagttctaa
      841 taactcccat aaactctttt cccataaact tttttaattg ccaaacacca taatcttgag
      901 ccattttttt atcttcatct gctaaaagag taattgctaa atcatacttt tctataaatt
      961 ttctatgttt tgcactatca tctgggctta caccaataac tgttgctttt agattgtcaa
     1021 acttatataa gtttgcagta aaatcacaag cttgtgtagt acatcctggt gtcatatctt
     1081 ttggataaaa gtataaaact atccaatttc caacaatatc tcttagacaa atctctgtat
     1141 catcttgatt aaaagcacaa aatgctggtg ctttgtcgcc tacttttaac atatattctc
     1201 ctttcaaatt ggttccctaa tttgaaaggg aaccgttatt atttaacttc ttttaaaaga
     1261 agcaaactct aaagggcttt aagaataaag ccttttacta tttaattg
//
LOCUS       DAOQAN010000453         1303 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_165311_length_1303_cov_3.076122, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000453 DAOQAN010000000
VERSION     DAOQAN010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1303
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_165311_length_1303_cov_3.076122"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..774)
                     /gene="ribD"
                     /locus_tag="PKO10_06890"
     CDS             complement(<1..774)
                     /gene="ribD"
                     /locus_tag="PKO10_06890"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HNW66910.1"
                     /translation="MKLAIDEAWKYQLLTYPNPAVGCVVVKDGKLLAIEAHKEVGMPH
                     AEVNALKTALLSKESNSLLRALTKSSEIHEYLIKNHNNFFNDCEIYTTLEPCNHEGKT
                     PSCAKLLSILKPKRVIIGSIDTNKIASGGIKTLEDENINVTTKVLEKECENLLIPFKS
                     WQNKTSIFFKMAQTLNGSIDGQISSQRAKLYVHTLRDKIDLLLIGGNTIRTDKPTLDT
                     RYIKGREPDIFIYSKNKVFSQNIPLFKVPNRKVIISDDLF"
     gene            complement(812..1240)
                     /gene="rimP"
                     /locus_tag="PKO10_06895"
     CDS             complement(812..1240)
                     /gene="rimP"
                     /locus_tag="PKO10_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HNW66911.1"
                     /translation="MNLEEQIKLIVENSGLKLYDIVTTKEHERNIFRVIVTSKDGVNL
                     DKCAEISRLISPILDIDEPMGGKYNLEVSSPGIERKLKKPEHFIASVGELVKVKNIET
                     DLFKGELLNADNEKITIKTQFADEEITYDNILSAATYFEW"
BASE COUNT          401 a          212 c          159 g          531 t
ORIGIN      
        1 aaatagatca tcactgatga ttacttttct atttggtact ttaaaaagtg gtatgttttg
       61 agaaaaaacc ttatttttac tataaataaa aatatctggt tctcttcctt ttatatatct
      121 tgtatctaaa gttggtttat cagttcttat agtgtttcca ccaattaaaa gaaggtctat
      181 tttgtctctt aatgtgtgaa catatagctt tgctctttga gagcttattt gtccatctat
      241 agagccattt aaagtttgtg ccattttgaa aaaaatagat gttttatttt gccaagattt
      301 aaaaggaatt aaaagatttt cacactcttt ttctaaaacc tttgttgtca catttatatt
      361 ttcatcttct aatgttttta ttccaccact tgcaatttta tttgtatcaa tagagccaat
      421 aattactctt ttgggtttta aaatagataa aagttttgca caagatggag ttttcccttc
      481 atgattacaa ggttctaaag ttgtatatat ttcacaatca ttaaaaaagt tattatgatt
      541 tttaattaaa tactcgtgta tttcagaact ttttgttaag gctcttaata aagagttaga
      601 ttctttgcta agaagtgctg tttttaaagc atttacttca gcatggggca ttcctacttc
      661 tttgtgcgct tcaattgcta aaagctttcc atcttttaca acaacacatc caacagcagg
      721 attgggataa gttaaaagtt gatatttcca agcctcatca atggctaatt tcatatagaa
      781 attatcatca atcttcacaa aaaactactt tttaccactc aaaatatgtt gcggcactta
      841 agatattatc ataagtaatc tcttcatctg cgaactgcgt ttttatagta attttttcat
      901 tatcagcatt caaaagttca cctttgaaaa gatctgtttc aatatttttt acttttacaa
      961 gctcaccaac acttgctata aaatgttcag gcttttttaa ttttctctca attccaggag
     1021 agcttacttc aagattatac tttccaccca ttggttcatc aatatctaaa atcggagata
     1081 ttaatcttga gatttcagca catttatcta aatttacacc atcttttgat gttacaataa
     1141 ctctaaaaat atttctctca tgctctttag ttgtaacaat atcataaagt tttaaaccac
     1201 tattttcaac tattagttta atttgttctt ctaaattcat tcttcttcac tttttgtttt
     1261 atcttttttg gaaacgaggg tttcaagttt ttcaatctct aag
//
LOCUS       DAOQAN010000454         1299 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_166091_length_1299_cov_3.110932, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000454 DAOQAN010000000
VERSION     DAOQAN010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_166091_length_1299_cov_3.110932"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1299)
                     /locus_tag="PKO10_06900"
     CDS             complement(<1..>1299)
                     /locus_tag="PKO10_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HNW66912.1"
                     /translation="KLKLFYIGKEKIDGQITPLVYQNKDLLTHAAIIGMTGSGKTGLG
                     ITLLEEAAIDAIPSIIIDPKGDMTNLLLNFPELNPSDFEPWLDDSEVSNSGGTKEELA
                     IKVSNSYKEGIQRDFQDLSRVKKLKDSADFTIYTPGSSAGVQVSILSSFKAPTIEILE
                     DMELFSNIINSTVHSILSLIDNKSDETSKESILLASIFTNYFKEQKDLTLEELITLIV
                     TPPFSKIGVFDLETFFPQSDRLKFALKINTIIASPSFSSWLEGESLDISKMLYSQSGK
                     AKVNIFSIAHLNDSQRMFFVTILLNQILAWMRRQEGTTSLKALLYMDEIFGYFPPQAN
                     PPSKQPMLTLLKQARSFGIGVILSTQNPVDIDYKGLSNIGTWFIGRLQTKQDIEKVID
                     GLSSASENGLNKQELSLALGTLAKRNFIMKNINEESIKTFE"
BASE COUNT          406 a          205 c          169 g          519 t
ORIGIN      
        1 ttcaaaagtt tttatactct cttcatttat atttttcata ataaagtttc ttttagcaag
       61 tgttcccaaa gctaaagata actcttgttt atttaatcca ttttcactag cactacttaa
      121 accatcaatt actttttcta tatcttgttt agtttgaagt ctacctataa accaagttcc
      181 tatatttgat aatcctttat aatcaatatc tacaggattt tgagttgaaa gaataactcc
      241 tattccaaaa cttcttgctt gttttaaaag tgttagcatt ggttgttttg atggaggatt
      301 tgcttgagga ggaaaatatc caaaaatctc atccatataa agtagggctt ttaaagaggt
      361 agttccctct tgtcttctca tccaagctaa aatttgattt agtaaaattg ttacaaaaaa
      421 cattctttgt gagtcattta gatgtgcaat tgaaaaaata tttactttag cttttccact
      481 ttggctatat aacatttttg atatatctaa actctcacct tctaaccaac tactaaaaga
      541 tggacttgca ataattgtat ttatttttag tgcaaatttt agtctatcac tttgaggaaa
      601 aaaagtctcc aaatcaaaaa ctccaatttt tgaaaaaggt ggagttacta ttaaagttat
      661 taactcttct agagttaaat ctttttgttc tttaaaataa tttgtaaaaa tagatgctaa
      721 aagaattgac tcttttgatg tttcatcgct tttattatct attaaagata agattgagtg
      781 tactgttgag tttataatat ttgaaaacaa ctccatatct tctaaaatct ctattgtagg
      841 tgctttaaaa gatgaaagaa ttgatacttg aactccagcg ctgcttcctg gagtgtatat
      901 tgtgaaatca gcactatctt ttagtttttt tactctactt aaatcttgaa aatctctttg
      961 tattccttct ttgtagctat ttgaaacttt tatagctaac tcttcttttg ttcctccact
     1021 atttgatact tcactatcat ctaaccaagg ttcaaaatca cttggattta attctggaaa
     1081 atttaacaga agatttgtca tatctccttt ggggtcaatt ataattgatg gtatggcatc
     1141 aatagcagct tcttctaaaa gcgttattcc aagtcctgtt ttaccacttc ctgtcattcc
     1201 tataattgct gcatgagtta ataaatcttt gttttgataa actaaaggtg tgatttgacc
     1261 gtcaattttt tcttttccaa tataaaaaag ttttaactt
//
LOCUS       DAOQAN010000455         1296 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_166717_length_1296_cov_2.691378, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000455 DAOQAN010000000
VERSION     DAOQAN010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_166717_length_1296_cov_2.691378"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..972
                     /locus_tag="PKO10_06905"
     CDS             <1..972
                     /locus_tag="PKO10_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013136330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNW66913.1"
                     /translation="DKNIVKIEENSDFDDFENEFISKNDDKQYWFSQDKMELITKIHD
                     YLDNIPEVGKVQSLATLLKIGKTLNDNKDLDGITLALIYNQLPEDYKKLILSPFVNIE
                     ANEARISMRIIDSNDELRRNELIKKINRDLEDIITNKDTTFRLTNLMILYNNMLQSLF
                     ESQISTAGASIFILGIMFLILFKNIKMVLIALITNLIPISLVFGIMGWLKIPLDIMTI
                     TIAAIALGIAVDDTIHFMHRFDFEYKQSHGNYKVSINKAINTVGHPMYHTTIIVVIGF
                     SILMLSNLVPTIYFGLLTAVVMISVLIANLILLPRLLFIFKPFKTKN"
     gene            985..>1296
                     /locus_tag="PKO10_06910"
     CDS             985..>1296
                     /locus_tag="PKO10_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014474641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HNW66914.1"
                     /translation="MNVAIYCGSAFGNSKIYEEKTIELAEKLYKNSINIVYGGSKQGL
                     MGIVSNKSLSLGNKVTGVITFDLVGKELENTNISKIYKVDSMRERKAKMEELSDAFIA
                     LP"
BASE COUNT          514 a          142 c          190 g          450 t
ORIGIN      
        1 gataaaaata tagtaaaaat agaagaaaat agtgattttg atgattttga aaacgaattt
       61 atatcaaaaa atgatgataa acaatactgg ttttctcaag ataaaatgga gttaataaca
      121 aaaatacatg actatcttga taatattcct gaagttggaa aagttcaatc cttagcaact
      181 ttacttaaga ttggaaaaac tttaaatgat aataaagatt tagatggaat aactctagca
      241 cttatttaca atcaacttcc agaagattat aaaaaactta ttttaagccc ttttgtaaat
      301 attgaagcaa atgaagctag aattagtatg agaataattg actcaaatga tgagttaaga
      361 agaaatgaac ttataaaaaa gattaataga gatttagaag atattatcac aaacaaagat
      421 acaactttta gacttacaaa ccttatgatt ttatacaata atatgcttca atcactattt
      481 gaatctcaaa tttcaactgc tggagcttct atttttattt taggtattat gtttttaata
      541 ctttttaaaa atataaaaat ggttttaata gctttaatta caaatctaat tcctatctct
      601 cttgtttttg ggattatggg ttggcttaaa ataccactag atataatgac aattacaatt
      661 gcagctattg ctttaggaat tgctgttgat gatactattc attttatgca tagatttgat
      721 tttgagtaca aacaaagtca tggaaactat aaagtatcaa taaataaagc aataaataca
      781 gttggtcatc ctatgtatca tacaacaatt attgttgtta ttggattctc aatcttaatg
      841 ctttcaaatc ttgttccaac tatatatttt gggcttttaa cagcagttgt aatgattagt
      901 gttttaatag caaatcttat tttacttcca agattgctat ttatatttaa accatttaaa
      961 acaaagaatt aggagaatta atttatgaat gtagcgattt attgtggttc tgcttttgga
     1021 aatagtaaaa tatatgaaga aaaaactata gaacttgcag aaaaattata taaaaacagt
     1081 ataaatattg tttatggcgg ttcaaaacaa ggattaatgg gaattgtctc aaataaatct
     1141 ttaagtttag gaaataaagt cacaggtgta atcacttttg atttagttgg aaaagagctt
     1201 gaaaatacaa atatttcaaa aatatataaa gtagatagta tgagagagag aaaagcaaaa
     1261 atggaggagc taagcgatgc ttttatagct cttcca
//
LOCUS       DAOQAN010000456         1294 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_167247_length_1294_cov_1.655367, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000456 DAOQAN010000000
VERSION     DAOQAN010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_167247_length_1294_cov_1.655367"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /gene="prs"
                     /locus_tag="PKO10_06915"
     CDS             <1..395
                     /gene="prs"
                     /locus_tag="PKO10_06915"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014473337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HNW66915.1"
                     /translation="LKNYANYDFDDKKREKANESQVMNIIGDVKGKDVILVDDMVDTA
                     GTLVKAAEVLKEKGANSVMACCTHGVLSGPAYERVENGVLDELVISDTIPTKKNAKKI
                     TVLTASSIIGEAIRRIHNNESVNSIFNN"
     gene            468..>1294
                     /locus_tag="PKO10_06920"
     CDS             468..>1294
                     /locus_tag="PKO10_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNW66916.1"
                     /translation="MKKILLSSLACASLVLAANSDYKYEITPLIGGGLGEGNHSLERN
                     YANAGLALGFNQSEDSLIDQFELGFLRTVQDVDGKNAVRNQDTSITRVFGNLVKDYGL
                     TTDLSLYALAGLGVEFFDHELTKHQKDGLFGNYGVGLKYQLTDAMALKFDLRHLISAQ
                     NGDSTLLYNFGLAIPFGEKAAKVAPVAAPAPVAAKAAPKDSDGDGVIDELDKCPDTMR
                     GSKVDTVGCMTLVNLNINFDTAKADIKDSYNSGIAEFAKVMNTDKNLKANIEAHTDSV
                     "
BASE COUNT          489 a          152 c          239 g          414 t
ORIGIN      
        1 ttcttaaaaa ctatgcaaat tacgattttg atgataaaaa aagagagaaa gcaaatgagt
       61 ctcaagttat gaacataatt ggagatgtaa aaggtaaaga tgttatttta gtagatgata
      121 tggttgatac tgctggaact ttagtaaaag ctgctgaagt tttaaaagaa aaaggagcta
      181 acagtgttat ggcatgttgt acacatggag ttttaagtgg gccagcttat gaaagagttg
      241 aaaatggtgt tttagatgaa ttggttatct cagatacaat tccaactaaa aaaaatgcaa
      301 aaaaaataac tgtgctaaca gcttcttcta taataggtga agcaataaga agaatacata
      361 ataacgaatc agtaaattct atttttaata attaaacaaa cgttaataaa aaattaataa
      421 attactgata taattcgcgt attgtaattt aaaattaagg aaaagaaatg aaaaaaatat
      481 tactatcttc attagcttgt gcatctttag tacttgctgc aaatagtgac tacaaatatg
      541 aaatcactcc attaatcggt ggaggattag gtgaaggtaa tcatagttta gaaagaaact
      601 atgcaaatgc tggattagct ttaggtttta atcaatcaga ggattcatta attgatcaat
      661 ttgaattagg ttttttaaga acagttcaag atgttgatgg aaaaaatgct gtaagaaatc
      721 aagatacttc aattactaga gtatttggta acttagttaa agattacggt ttgactactg
      781 atttatcttt atacgcatta gcaggacttg gagttgagtt ttttgatcat gagttaacta
      841 aacaccaaaa agatggtcta tttggaaact atggagttgg tttaaaatat caattaacag
      901 atgcaatggc tttaaaattt gatttaagac atttaatatc tgcacaaaat ggagacagta
      961 ctttattata taactttggt ttagctattc catttggtga aaaagctgca aaagttgctc
     1021 ctgtagctgc tcctgctcct gtagctgcaa aagctgctcc aaaagatagc gatggagatg
     1081 gtgttattga tgaacttgac aaatgtccag atacaatgag aggttcaaaa gttgatactg
     1141 ttggatgtat gactttagtt aatttaaata ttaactttga tactgctaaa gcagatatta
     1201 aagactctta caactcagga attgctgaat ttgcaaaagt tatgaataca gataaaaact
     1261 taaaagcaaa tatcgaagct catactgatt cagt
//
LOCUS       DAOQAN010000457         1293 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_167435_length_1293_cov_1.848142, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000457 DAOQAN010000000
VERSION     DAOQAN010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_167435_length_1293_cov_1.848142"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /gene="hemC"
                     /locus_tag="PKO10_06925"
     CDS             <1..484
                     /gene="hemC"
                     /locus_tag="PKO10_06925"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468102.1"
                     /note="transformation of porphobilinogen to
                     hydroxymethylbilane in porphyrin biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HNW66917.1"
                     /translation="RIAKLNAGEYDAIILAATGIQKLGIENEVKYFTPISTDVMIPSM
                     GQATLGIETTNDPKIIEIVKVLNDKNAQIESTIERGFVDTLQGGCQVPIGVKATIQDE
                     KSVKISAIVGLPDGSEYIKDEKIIDINSFESAGRDFANEFIKKGAVELLKRAEAMAFK
                     "
     gene            601..1143
                     /locus_tag="PKO10_06930"
     CDS             601..1143
                     /locus_tag="PKO10_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HNW66918.1"
                     /translation="MRIKIEDTLFCLVDVQEKLFPHIGNKDELLRTLPILVKGMKTLD
                     VPIIVNEQYKKGIGETIEPLKELVTEYTAYEKTTFSGCQNSDILNAFKKSGKKNIVVA
                     GIETHVCVLQTCIDLLENRFNVILVTNCCGSRTKLDHKMAIKRLIQSGVVPTTYESIL
                     FELTLDSKNPNFKEISSLIK"
BASE COUNT          505 a          154 c          204 g          430 t
ORIGIN      
        1 tagaattgct aagttaaatg caggtgaata tgatgcaatt attcttgcag ctacaggaat
       61 tcagaaactt ggaattgaaa atgaagttaa atactttact cctatttcaa cagatgttat
      121 gattccatct atgggacaag caactttagg aattgagaca acaaatgatc caaaaataat
      181 tgagatagta aaagttttaa atgataaaaa tgcacaaatt gaatcaacaa ttgagagagg
      241 ttttgtagat actttacaag gtggttgtca agttccaatt ggtgtaaaag caacaattca
      301 agatgaaaaa agtgtaaaaa tatctgcaat tgtaggactt cctgatggaa gtgaatatat
      361 aaaagatgaa aaaattatag atataaactc ttttgagagt gctggaagag attttgcaaa
      421 tgagtttata aaaaaaggtg ctgttgagct tctaaaaaga gctgaagcta tggcttttaa
      481 ataataattt ctatgattta aaagctagtt tctagctaca caattacgat atatttcttt
      541 ttcaagactt tattaaatga acggttcctt taaaatgtga accatttaaa aggagaaaat
      601 atgagaataa aaatagaaga tacacttttt tgtttagttg atgttcaaga aaagcttttc
      661 ccacatattg gaaacaaaga tgagctttta agaactcttc caatacttgt aaagggtatg
      721 aaaactttag atgttcctat tattgtaaat gaacagtata aaaaaggaat tggtgaaaca
      781 atagagcctt taaaagagct tgtaactgaa tacactgctt atgaaaaaac taccttttca
      841 ggatgtcaaa atagtgatat tttaaatgct tttaaaaaat ctggtaaaaa aaatattgtt
      901 gttgctggaa tagagactca tgtttgtgtt cttcaaactt gtattgattt acttgaaaat
      961 agatttaatg ttattttagt aacaaactgt tgtggaagta gaacaaagct tgaccacaaa
     1021 atggcaatta aaagattaat tcaatcaggt gtagttccta caacttatga atcaatttta
     1081 tttgagctaa ctttagattc taaaaatcca aactttaaag aaatatcttc actaataaaa
     1141 tagtttaaaa ctgggttaaa taaccctttt ttaaactact ccctattaca aatagtttta
     1201 taagtacaat aagtacaaat agctttatct tcagttttac aaaaatcaat tttactttta
     1261 cattgatatt ttaaatcatc aaagacagtt tgt
//
LOCUS       DAOQAN010000458         1290 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_168041_length_1290_cov_1.578947, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000458 DAOQAN010000000
VERSION     DAOQAN010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1290
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_168041_length_1290_cov_1.578947"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            71..>1290
                     /locus_tag="PKO10_06935"
     CDS             71..>1290
                     /locus_tag="PKO10_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3971 domain-containing protein"
                     /protein_id="HNW66919.1"
                     /translation="MSYNGVFIAEKSNISISGFDFETNGAKVILENDLLYINDASFIY
                     QDIVDAKANLKLDLNSLKIEGTSQINKILVKDSKNSKILNITNANSDIFMDFSKNVNI
                     ELKDLKTVIKYDKFLTINLNDISKIYNYSDILKQNSINSGNISLKIINKDNIDFNGFF
                     SGFTIPIKKDNKEINSLDFYGNINKESVQISSSDNSIRLNFKDNIDLFLDGYDIFYDE
                     NISKNSKLEFDTNIDLKNFKLFFDNQEYKIKSANFNIKKDLITFNASLFDLDLPIKKD
                     NQDLKELDILGKYKNDILDINSKKDDIFLRVRDNQTTIKLDGYDIFYDTKYNDIHEKI
                     GNLGIIGTKSNIVINNKNKILADFYELNINKDRKFFYLEFEDSKISFTNQNDKIDIYA
                     AKINEKFINTFANS"
BASE COUNT          579 a           87 c          163 g          461 t
ORIGIN      
        1 tttgctcttg ctcatttagt attcgggaat tgggttactt ttatcttttc cttatgcaga
       61 agataagcct atgagttata atggagtttt tatagcagaa aaatcaaaca tttctatatc
      121 tggttttgat tttgaaacaa atggtgcaaa agttatttta gaaaatgatt tgttatatat
      181 aaatgatgct agttttattt atcaagatat tgtagatgca aaagcaaatt taaaactaga
      241 tttaaatagt ttaaaaatag aaggaacttc acaaataaat aagattttag taaaagatag
      301 caaaaattct aaaattttaa atataacaaa cgcaaatagt gatattttta tggattttag
      361 caaaaatgta aatattgaac taaaagattt aaaaacagtt ataaaatacg ataagttttt
      421 aactataaat ctaaatgata tttcaaagat atataattat tcagatattt taaaacaaaa
      481 tagtataaat agtggaaata tatctttaaa aattataaat aaagataata tagatttcaa
      541 tggatttttt agtggattta ctattcctat aaaaaaagat aacaaagaga taaatagttt
      601 agatttttat ggaaatatta ataaagagag tgtacaaatc tcttctagtg ataactcaat
      661 aaggttaaat tttaaagata atatagatct ttttttagat ggatatgata ttttttatga
      721 tgagaatatc agtaaaaata gtaaattaga atttgataca aatatagact taaaaaattt
      781 taaactattt tttgataatc aagagtataa aataaaaagt gctaatttta atataaaaaa
      841 agatttaata acttttaatg catctttatt tgatttggat ttacctataa aaaaagataa
      901 tcaagattta aaagagcttg atattttagg aaagtacaaa aatgatattt tagatataaa
      961 ctcaaaaaaa gatgatattt ttttaagagt aagagataat caaacaacta taaaattaga
     1021 tggatatgat atcttttatg atacaaaata taatgatatt catgaaaaaa taggtaattt
     1081 aggtattatt ggaacaaaat caaatatagt tattaataat aaaaataaga tattagcaga
     1141 tttttatgag ttaaatataa ataaagatag aaaatttttc tatttagaat ttgaagatag
     1201 taaaataagt tttacaaatc aaaacgataa aatagatata tatgcagcaa agataaatga
     1261 aaaatttata aatacttttg caaatagtaa
//
LOCUS       DAOQAN010000459         1289 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_168150_length_1289_cov_2.478120, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000459 DAOQAN010000000
VERSION     DAOQAN010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_168150_length_1289_cov_2.478120"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1289)
                     /locus_tag="PKO10_06940"
     CDS             complement(<1..>1289)
                     /locus_tag="PKO10_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HNW66920.1"
                     /translation="KFTSNTLYAILTGFLSTSVVQSSTIITLIVVSFLSAELLTLVQA
                     VGIVFGSNVGSTTTAWIVSSLGVDVKISTYAFPMLVFGVVLRFFKSNGVKGSGNVLLG
                     LGFIFLGISYMKDGFDIMKNSIDLASYAMEGYLGIIVYILIGILITVVIQSSAATLAI
                     VITALNADSITYVNAIALAIGANVGTTLTTILASFASNENGKRVALIHFLFNLISATF
                     ITILIYQFIDLTDFIAPFLGVSDNNYGMKLALFHTIFSVTGVILLTPAISLLVKLSEK
                     LIQKKVSSASKPKYLNQSVLSNPDASLAALKKEIINLYENCQKAMLHALNIHTTGLTR
                     ETLKVQLAKELTIIDTDIDEIYQKNLKALYSEIVKFSSFAQENMFDFQHKKVDELKRA
                     AVLLVEVLRDTRDIQKNVNFYLKSKNEYIKEEYNILR"
BASE COUNT          478 a          192 c          160 g          459 t
ORIGIN      
        1 tttcttagaa tattatactc ctcttttata tactcatttt tactttttaa gtaaaaattt
       61 acattttttt gaatatctct tgtatctctt aacacctcta caagtaaaac agcggcacgt
      121 tttagttcat caactttttt gtgttgaaaa tcaaacatat tttcttgtgc aaaagatgaa
      181 aattttacaa tttcactata aagtgcttta agattttttt gataaatctc atcaatatct
      241 gtatctataa tagtcaactc ttttgctaat tgaactttta gtgtctctct tgtaagtcct
      301 gttgtatgta tatttaaagc atgtaacata gctttttgac aattttcata tagatttata
      361 atctcttttt taagagctgc taaagaggca tcaggatttg ataaaacaga ttgattaaga
      421 tattttggtt ttgaagcaga agataccttt ttttgtatca atttttctga taattttact
      481 aataaagata ttgctggagt tagtaaaata actccagtta cagaaaaaat agtatggaaa
      541 agtgctaatt tcataccata attattatcg ctaactccta aaaatggtgc tataaaatct
      601 gttaaatcta taaattgata gattaaaata gttataaatg ttgctgaaat tagattaaat
      661 aagaagtgaa taagagctac tctttttcca ttttcatttg aagcaaatga tgctaaaatt
      721 gttgtaagtg ttgtaccaac atttgctcca attgctagtg ctattgcatt tacataagta
      781 atactatctg catttaaagc tgtaataact atagctaaag ttgcagcact tgattgtata
      841 acaacagtaa ttaaaattcc aattaagata taaacaataa ttcctaaata tccttccata
      901 gcgtaagatg ctaaatcgat agaatttttc ataatatcaa aaccatcttt catatatgaa
      961 ataccaagga aaataaatcc taaacctaaa agaacatttc cgctcccttt tactccattt
     1021 gatttaaaaa atcttaaaac aactccaaaa acaagcatag gaaaagcata agttgaaatt
     1081 tttacatcaa ctcctaagct tgaaactatc caagcagttg ttgtgcttcc tacatttgaa
     1141 ccaaaaacaa taccaactgc ttgaactaaa gtaagaagtt cggcacttaa aaaagataca
     1201 acaattaaag taataattgt tgaactttga accactgaag ttgataaaaa tccagtcaaa
     1261 atagcataaa gagtattgct agtaaactt
//
LOCUS       DAOQAN010000460         1287 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_168600_length_1287_cov_2.396916, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000460 DAOQAN010000000
VERSION     DAOQAN010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_168600_length_1287_cov_2.396916"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="PKO10_06945"
     CDS             complement(<1..623)
                     /locus_tag="PKO10_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNW66921.1"
                     /translation="MTKKVSLDLKKRLSKEDALDLIKNASLLDLAKMASAKKDELHKE
                     KLTTFIVDRNINYTNVCWVDCKFCAFFRHKKDNDSYVLKFDEIDEKIEELLAIGGTQI
                     LFQGGVHPNLKIDYYEELVNHIHTKYPQITIHGFSSIEIDFIAKVSKISILEVLKRLQ
                     AKGLSSIPGAGAEILSDRVRDIIAPKKMDTQEGLEVHRLAHSIGMKTT"
     gene            complement(623..>1287)
                     /locus_tag="PKO10_06950"
     CDS             complement(623..>1287)
                     /locus_tag="PKO10_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HNW66922.1"
                     /translation="LNTYNAQSQTFKDKNKLFQDQDYTNSSITPYINYDSTNDFYFPT
                     AGIKAGASVEYAGVGGDSKYIKPGVNFRYFYSLEDLTELDWILRFKTQAKMLIDEGQI
                     NQGDSLYLGGPKTLRGYKSYAFPNNESGYYQAPYEKMWSNQAEISFPLVPSAKMRWGL
                     FYDYGMIGQNSFSEIKRSGTGALLEWISPMGPLQLIFAKPIGDKPGDDTSSFEFSFGT
                     SF"
BASE COUNT          400 a          219 c          166 g          502 t
ORIGIN      
        1 gttgttttca ttccaataga gtgtgctagt ctatgaactt ctaacccctc ttgtgtatcc
       61 attttttttg gagcaattat atctcttact ctatcgctta aaatctcagc tccagctcct
      121 ggaattgagc ttaaaccttt tgcttgaagt ctttttaaaa cttctagtat tgagattttt
      181 gaaacttttg ctataaaatc aatttcaata gatgaaaaac cgtgaattgt aatttgtgga
      241 tattttgtat gaatgtggtt tactaactct tcataataat caattttcaa atttggatga
      301 actccacctt gaaaaagtat ttgagttcca ccaattgcta aaagttcttc tattttttca
      361 tcaatttcat caaattttaa aacataacta tcattatctt ttttgtgtct aaaaaaagca
      421 caaaacttac aatcaaccca acatacattt gtatagttta tatttctatc tactataaaa
      481 gtagttagtt tttctttatg aagctcatct ttttttgcac ttgccatctt cgctaaatct
      541 aacaaagatg cattttttat taaatcaagt gcatcttctt tacttaatct tttttttaaa
      601 tcaagcgata ctttttttgt cattagaaac ttgttccaaa agagaactca aaacttgatg
      661 tatcgtcacc tggtttatca ccaattggtt tagcaaatat taattgtaaa ggacccattg
      721 gagaaatcca ctctagtaaa gctcctgttc ctgatctttt tatctcacta aagctatttt
      781 gacctatcat accataatca taaaatagtc cccatctcat ttttgcagat ggaactagag
      841 ggaaactaat ttctgcttga tttgaccaca ttttttcata aggggcttga taatatccag
      901 attcattatt tgggaaagca taagatttat atcctcttaa agtttttgga cctccaagat
      961 ataaagagtc tccttgattt atttgacctt catctattaa catttttgct tgagttttaa
     1021 atcttaaaat ccagtcaagc tctgttaaat cttctaaaga gtagaaatat ctaaaattaa
     1081 cccctggttt aatatatttt gaatctccac caactcctgc atattcaaca gaagccccag
     1141 cttttatacc agctgttgga aaatagaaat catttgtact atcatagttt atgtaaggtg
     1201 taatagagct atttgtatag tcttgatctt gaaataattt atttttatct ttaaatgttt
     1261 gagattgtgc attgtaagta tttaaga
//
LOCUS       DAOQAN010000461         1287 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_168640_length_1287_cov_2.017045, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000461 DAOQAN010000000
VERSION     DAOQAN010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_168640_length_1287_cov_2.017045"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="PKO10_06955"
     CDS             <1..501
                     /locus_tag="PKO10_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66923.1"
                     /translation="RYAARVQDAVTLKYNNNFLDSSLITSKYYIDILRAFGVFSRVDF
                     NIDGNNNPSYLVTKAYSDLYLSKPLNSIETLKRLKDEFGYENRFTMYLSTASFLESLR
                     PEEASIQISLIKAFYKDKDTDFLTAIQLLQNMNISSAKQFLVNKYNNPYIDFRIVGFE
                     EFMLSL"
     gene            558..872
                     /locus_tag="PKO10_06960"
     CDS             558..872
                     /locus_tag="PKO10_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HNW66924.1"
                     /translation="MEIGRVAQIDNAKENYEIKTKQVSSVEEKVKTIQEDEYKKNSSK
                     EPTSELNEVILDNIKFGYNRESKDFFVKITRGDIENKYPTEDMMKIKAFLIEELEKSI
                     KN"
     gene            889..>1287
                     /locus_tag="PKO10_06965"
     CDS             889..>1287
                     /locus_tag="PKO10_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014475092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliY"
                     /protein_id="HNW66925.1"
                     /translation="MASDLSNVFKNELSNTLEQLLGKKAKISETKKLDDIFDSSSFIE
                     ADVKFDIKGLSSAIVFYIPTISATKFEYLMLGGMGDLKEDIDDETTDAVNEIVSNICG
                     SFCTASNAQGMPDIVGMKSEIKGTKKVEKSA"
BASE COUNT          531 a          118 c          215 g          423 t
ORIGIN      
        1 agatacgcag caagagttca agatgcagta actttgaaat ataataataa ttttttagat
       61 agctctttaa ttacttcaaa atattatatt gatattttaa gagcatttgg tgttttttca
      121 agagtagatt ttaatataga tggaaataat aacccaagtt atttggtaac aaaagcttat
      181 agtgatttat atttgagtaa accacttaat tctatagaga cattaaagag actaaaagat
      241 gagtttggat atgaaaatag atttacaatg tatctttcaa ctgcatcttt tttagaatct
      301 ttaagacctg aagaggcatc tattcaaata tctttaataa aagcatttta taaagataaa
      361 gatacagatt ttttaactgc aattcaactt ttacaaaata tgaacatttc aagtgcaaag
      421 caatttttag taaataagta taataaccca tatatagatt ttagaattgt tggttttgaa
      481 gagtttatgc tttctttata atactattta attttttttt gttataatgt tttaaaattc
      541 ttacaaaggg gtctttagtg gaaattggaa gagtagcaca aattgataac gcaaaagaaa
      601 actatgagat aaagacaaaa caagtaagca gtgttgaaga aaaagttaaa acaatacaag
      661 aagatgagta taaaaagaac tcttcaaaag aaccaacaag tgagttgaat gaggttattt
      721 tggataatat taagtttgga tacaacagag agtcaaaaga tttttttgta aaaataacaa
      781 gaggcgatat tgaaaataaa tatccaacag aagatatgat gaaaataaaa gcttttttaa
      841 ttgaagagct agaaaaaagt attaaaaatt aaaaaaaggt aagatatttt ggcatcagat
      901 ttatcaaatg tatttaaaaa cgagctatca aatacactag agcagttatt agggaagaaa
      961 gcaaaaattt ctgaaacaaa aaaattagat gatatttttg attcttcaag ttttatagaa
     1021 gcagatgtaa aatttgatat aaaaggttta agttcagcta tagtttttta tattccaact
     1081 attagtgcta caaagtttga atatttaatg ttaggtggaa tgggtgattt aaaagaagat
     1141 attgatgatg aaacaacaga tgctgtaaat gagatagttt caaatatttg tggaagtttt
     1201 tgtactgctt caaatgctca aggtatgcca gatattgtag gtatgaaatc agagattaaa
     1261 ggaacaaaaa aggtagaaaa aagtgca
//
LOCUS       DAOQAN010000462         1280 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_170064_length_1280_cov_2.231837, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000462 DAOQAN010000000
VERSION     DAOQAN010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_170064_length_1280_cov_2.231837"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..883)
                     /locus_tag="PKO10_06970"
     CDS             complement(56..883)
                     /locus_tag="PKO10_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012011949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNW66926.1"
                     /translation="MHKFLLALVLGSSLTYASTINGIAITVNDEPITQYDIEKTMSVN
                     KISKNEAVSYLIDKVLYDQLVKKNNITADIFETNEYIEKLANSNGMDVYAFKSIVKQE
                     YPNYEVFENEAKNAVIRQKLISSLVRGQLAIANDEDMQLYYEKNINKYLTSKYFDVVQ
                     YSSTNRNALVDTIQNPSKVASGEVTKVPLKLESNKIHAQLQYILNGTKPNTFTPIFTA
                     DKQYVTLFLKQAEGKVAIPFEVAKPRVFNDIMEQREQTFLKDHFEKQKVKADIKVLR"
     gene            complement(963..>1280)
                     /locus_tag="PKO10_06975"
     CDS             complement(963..>1280)
                     /locus_tag="PKO10_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014467927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNW66927.1"
                     /translation="EPITIHTTCSSRKMGLEDKFKKLANLCSTNVIVPADVKCCGFAG
                     DRGFNFPELNDSALRYLKEQTNGAKMAFSTSKTCEIGLSEKSGLDYNSIFYLINRVTK
                     ERS"
BASE COUNT          401 a          203 c          153 g          523 t
ORIGIN      
        1 acatgaactt ctaaaccaat tactacttca aacatacttg acatatttta aatccttatc
       61 ttaatacttt tatatctgct tttacttttt gtttttcaaa atgatctttc aaaaatgtct
      121 gttctctttg ctccataata tcattaaaca ctctaggttt tgcaacttca aaaggaattg
      181 caactttccc ttctgcttgt tttaaaaata gagttacata ctgcttatct gctgtaaata
      241 ttggagtaaa tgtatttggt tttgtaccat ttaaaatata ttgtagctga gcatgaattt
      301 tattactctc taattttaat ggcacttttg taacttcacc tgaagccact tttgatggat
      361 tttgaattgt atcaacaagt gcatttctat ttgtagagct atattgaaca acatcaaaat
      421 attttgaagt taaatattta tttatatttt tttcataata tagttgcata tcttcatcat
      481 ttgcaattgc taattgccca cgaaccaaac tactaataag tttttgtcta ataacagcat
      541 ttttcgcctc attttcgaaa acttcataat ttggatactc ttgtttaact attgatttaa
      601 atgcatatac atccattcca tttgagtttg caagtttttc aatatactca tttgtttcaa
      661 aaatatcagc agtgatatta tttttcttaa ctagttgatc atataaaacc ttatcaatta
      721 agtaactaac tgcctcattt tttgagattt tatttacaga cattgttttt tcaatatcat
      781 actgtgtgat tggttcatca ttaacagtta ttgctatacc gtttattgtt gaagcgtatg
      841 ttaagcttga gcctaatact aaagctagta gaaatttgtg cattttgaga atcctttctt
      901 tgataaaaga gatattttac caaaaaaaat gtaaatatca agtttgaaag gaataaagat
      961 ttttaactcc tttcttttgt aactctattt attagataaa aaatagagtt ataatcaagc
     1021 ccagattttt cactcaatcc tatttcacaa gttttacttg tagaaaatgc cattttagca
     1081 ccatttgttt gctcttttaa atatctaagc gctgaatcat ttaattctgg gaaattaaat
     1141 cctctatcgc ctgcaaatcc acaacatttt acatcagcag gaactattac atttgttgaa
     1201 cataaatttg ctaatttttt gaatttgtct tcaagtccca tttttcttga gctacaagtt
     1261 gtatgaattg ttattggttc
//
LOCUS       DAOQAN010000463         1277 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_170656_length_1277_cov_2.605565, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000463 DAOQAN010000000
VERSION     DAOQAN010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_170656_length_1277_cov_2.605565"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            32..784
                     /locus_tag="PKO10_06980"
                     /pseudo
     CDS             32..784
                     /locus_tag="PKO10_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014475492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
     gene            777..>1277
                     /locus_tag="PKO10_06985"
     CDS             777..>1277
                     /locus_tag="PKO10_06985"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNW66928.1"
                     /translation="MIRKSISLVVLSMLFSSCVSMAPKLDINSDEVVAKSFKNYQIAE
                     DNSNISLNSFLIDENLKTLVNLVLENNKDIKIALLRVEESKSLYRIEESNLYPKIDAN
                     GSFSRDKKEEIIKNNYKASVGTVFELDLFGKNRSLNDAAKNSFLATQYALSSTKLSLI
                     SQTINSY"
BASE COUNT          458 a          145 c          219 g          455 t
ORIGIN      
        1 tttatagcgt ttctctatga aatcctgtaa gaaagtaaga aatagtagtg gattaatggt
       61 tccttttagt gaatttagct cattttcttg ggtttatgca cctgaagagt taacaagatt
      121 taatggattt atctcttatg aaattcaagg aagtgcagca gctggagtta gctctggtgt
      181 tgctatgaat gagatggata aaattgcaaa tgaaaatgca aatggaacaa tgcatacata
      241 tagtggagct tcatatcaag aaaggttagc tagtaatcaa actttgattt tatatggaat
      301 ttctcttttg gttatatttt tatgtttagc tgcactttat gagagttgga gtgttccttt
      361 ctctgtttta ttggttgttc ctttaggagt ttttggagct gttttaagta tatattttag
      421 agacctaagc aacgatgtat attttcaagt agcactttta gcagttatgg gacttgcaag
      481 taaaaatgca attttaattg ttgaatttat aaatagtgca tataaaaatg gtatgccttt
      541 aatcgaagct gctataaaag gtgcaaaact aagactaaga ccaattatca tgacatcttt
      601 agcttttatt gctggaatta ttccacttgc aatatcaagt ggagctggag caaatagtag
      661 aattgcaata ggaactgcaa ttttaggtgg aacaataagt gcaacaattt tggcaatatt
      721 ttttgtacca ctattttttg taataattac aaaactattt tcaaaaggag ttaaaaatga
      781 ttagaaaatc tataagcctt gtagttttat caatgctttt tagctcttgt gtatcaatgg
      841 ctccaaaatt agatataaat agcgatgagg tagttgctaa aagttttaaa aactatcaaa
      901 tagctgaaga taattcaaat attagtttaa atagtttttt aatagatgaa aatttaaaga
      961 ctcttgtaaa tcttgtttta gaaaataata aagatataaa aattgcactt ttaagagtag
     1021 aagagtcaaa atcactttat agaatagaag agtcaaatct ttatccaaaa atagatgcaa
     1081 atggtagttt ttctagagat aaaaaagaag aaatcataaa aaacaactat aaagcatctg
     1141 ttggaactgt ttttgaacta gatttatttg gtaaaaatag aagtttaaat gatgctgcta
     1201 aaaatagttt tttagcaact caatatgctc ttagttcaac aaaattatct ttaatttcac
     1261 aaactataaa ttcatat
//
LOCUS       DAOQAN010000464         1276 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_170948_length_1276_cov_1.886159, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000464 DAOQAN010000000
VERSION     DAOQAN010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_170948_length_1276_cov_1.886159"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="PKO10_06990"
     CDS             complement(<1..374)
                     /locus_tag="PKO10_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012013126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HNW66929.1"
                     /translation="MNYEIKNSFIKAKIKSFGAELNSLQKIENDFEYIWQANSQYWAR
                     HSPVLFPIVGRLKNDSYFYKDKKYSLSQHGFARDKEFELIKKEDDFIEFSLKNDEETL
                     KNYPFLFELNISYKLEKTKLIIS"
     gene            complement(371..1132)
                     /gene="blaOXA"
                     /locus_tag="PKO10_06995"
     CDS             complement(371..1132)
                     /gene="blaOXA"
                     /locus_tag="PKO10_06995"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020848173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class D beta-lactamase"
                     /protein_id="HNW66930.1"
                     /translation="MYFKIKMILLAIFSINLFANDLEIEKIFKNKEVEGTMVIESLNQ
                     NKSYIYNKQRAETFLSPASSFKIPHTLIALNEGLVTVDSVINWDKNIGFVESCNANQT
                     LKSSFKNSCIWVYQVFASTIDSSKYKNYLEQMDYGNKVVGNDVKNFWLDESLKINAFE
                     QINFLKKLHKNNLPFKQNDIDALKNIMIDEKTSNYTIRSKTGWEGRYGWYVGYVETKD
                     DVWFFAMNIDTKSKDDLPKRKTITLETLRLKGIIN"
BASE COUNT          433 a          199 c          122 g          522 t
ORIGIN      
        1 gaaattatta gttttgtttt ctctaattta taagagatat ttaactcaaa taaaaatgga
       61 taatttttta aagtttcttc atcatttttt aaactaaatt ctataaaatc atcctctttt
      121 tttataagtt caaactcttt atctcttgca aaaccgtgtt gagataaact atatttttta
      181 tctttataaa aatagctatc attttttaat cttcctacta taggaaaaag cacaggtgag
      241 tgtcttgccc agtattgtga atttgcttgc caaatatatt caaaatcatt ctctattttt
      301 tgtaaactat ttaattcagc tccaaaagat ttgatttttg cttttataaa actatttttt
      361 atctcataat tcaattaata attcctttta atctcaatgt ttccaaagtt atagtttttc
      421 tttttggtaa atcatctttt gattttgtat caatattcat agcaaaaaac caaacatcat
      481 cttttgtctc aacatatcca acataccaac catatcttcc ttcccaacca gtttttgatc
      541 taattgtata attagatgtt ttttcatcta tcataatatt tttaagagca tctatatcat
      601 tttgcttaaa aggtaaattg tttttatgta gttttttcaa gaagtttatt tgttcaaaag
      661 cattgatttt caagctttca tcaagccaaa agttttttac atcatttcca acaactttat
      721 ttccataatc catttgttct aaatagttct tatattttga agaatctatt gttgaagcaa
      781 acacttgata aacccaaata caagagtttt taaaagagga ttttaaagtt tgattagcgt
      841 tacaagattc aacaaaacct atatttttat cccaattaat aactgaatct acagtaacta
      901 aaccctcatt taaagctatt aaagtatgtg gtattttaaa agatgaagca ggagacaaga
      961 aagtttctgc tctttgttta ttgtaaatat aactcttatt ttgatttaaa gattcaatta
     1021 ccattgttcc ttcaacttct ttatttttaa aaatcttttc aatctctaaa tcatttgcaa
     1081 aaagatttat tgaaaaaatc gccagtagaa tcatttttat tttaaagtac atatatcttc
     1141 cttattgttt tttttgtttt atttttttta aaattctcat agagttaaat atagcaagta
     1201 aagccacacc aacatctgca aaaatagcct ctttcatccc aataactgca cctgctccca
     1261 aaaataaaaa gccaat
//
LOCUS       DAOQAN010000465         1275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_171154_length_1275_cov_1.970492, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000465 DAOQAN010000000
VERSION     DAOQAN010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_171154_length_1275_cov_1.970492"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            248..937
                     /locus_tag="PKO10_07000"
     CDS             248..937
                     /locus_tag="PKO10_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014469068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02117 family protein"
                     /protein_id="HNW66931.1"
                     /translation="MFRKILKTLFFIILSIFLFVVVYLLSETILSKITTNYSKNENLD
                     YEIFIKSNGVHTDVVLPIKSDVINWFDFFNYEDTLSKRDDFLYISIGWGDKGFYLDTP
                     TWAELKLSTALIAGFGLGNAALHITYYDEIVEDDLTYKIKISKKQYSLIIDTIKNSLQ
                     YKDEKPINIKTTSQYGQNDAFYEAIGSYSIFHTCNTWTNNLLKKASLPASFWLAFDDG
                     ILYQYNKKSNP"
     gene            complement(979..>1275)
                     /locus_tag="PKO10_07005"
     CDS             complement(979..>1275)
                     /locus_tag="PKO10_07005"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNW66932.1"
                     /translation="CRVVEKDLRENDTFARIGGEEFLILLPNTNIEDAFYIAQRIRQN
                     IEQHKFKDIPKLTISLGVAEANEPILSTELLKKVDLALYKAKKEGRNKVIAFKN"
BASE COUNT          437 a          164 c          169 g          505 t
ORIGIN      
        1 tattaaatca atttgatgat tttccaaagc caatttttga gccatttgag ttgctttttc
       61 tataccttct ttagttaatt cagtatcata atcatattcg ttttgtgtct ctttttgttt
      121 tgttgtgtgt cttactataa aaagtttttt catttttccc tactttaaat aaaaatgcca
      181 gattttaact atatttttat aaattaataa taaaataatc aaaaatatct ataaggaaat
      241 attgttaatg tttagaaaaa ttttgaaaac tctatttttt attatcttat caatattttt
      301 atttgtagtt gtatatttat taagtgaaac tattctttca aaaattacaa caaattattc
      361 aaaaaatgaa aatctagatt atgaaatatt tataaaatca aatggagttc atacagatgt
      421 agttttacct ataaaaagtg atgtgataaa ctggtttgat tttttcaatt atgaagatac
      481 tttaagtaaa agagatgatt ttttgtatat tagtattggt tggggcgata aaggttttta
      541 tttagatacg ccaacttggg cagagttaaa actaagcact gctttaattg caggatttgg
      601 tcttggaaat gctgctttgc atattacata ttatgatgag attgttgaag atgatttgac
      661 gtacaaaata aaaatctcta aaaagcagta tagcttgatc attgatacaa taaaaaactc
      721 tttacaatac aaagatgaaa aaccaataaa tataaaaaca acatctcaat atgggcaaaa
      781 tgatgctttt tatgaggcaa tagggagtta tagtattttt catacttgta acacttggac
      841 aaataatttg ttgaaaaaag catctttacc agcctctttt tggctagcat ttgatgatgg
      901 aattttgtat cagtataata aaaaaagcaa cccttaacca aagagttgct ttaagatgaa
      961 attttaacca aatcaatctt aatttttaaa ggcgataact ttatttcttc cctctttttt
     1021 tgctttatat aaagcaagat ccactttttt tagaagttct gtacttaaga ttggttcatt
     1081 tgcctctgct acacccaaac ttattgttaa ttttggaata tctttgaact tatgttgttc
     1141 aatattttgt cttattcttt gagctatgta aaaggcatct tctatatttg tatttggtag
     1201 tagtattaaa aactcttccc caccaattct agcaaaagta tcattttctc ttaagtcttt
     1261 ttcaacaact ctaca
//
LOCUS       DAOQAN010000466         1273 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_171583_length_1273_cov_1.847291, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000466 DAOQAN010000000
VERSION     DAOQAN010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1273
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_171583_length_1273_cov_1.847291"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1273
                     /locus_tag="PKO10_07010"
     CDS             <1..>1273
                     /locus_tag="PKO10_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HNW66933.1"
                     /translation="HQHIMKIHRDIEDGNIKFAWVNVCNPYQDTANATHWVKAAREMD
                     NFIVTSDGYPGISAKVSDLILPSAMIYEKWGGYGNAERRTQLWRQQVVPVGDAMSDTW
                     QWVELSKRFTVKDVWGEQALLSTNNETKLPSLIEAAKAMGYNENTTMYEILFANDRAK
                     SYKADQKDEIQAGYDNTEAFGDSRNVLGSDGKPWKGYGFFLQKYLFEEYADFGRGHAH
                     DLADFDTYHRVRGLKWPVVDGKETSWRFNTKYDPYAKMANPNSDFAFYGTLAKELAQG
                     DLLGVKDQTKKSLKNKAKIFARPYMDPPEMPDEQYPVWLSTGRVLEHWHSGTMTMRVP
                     ELYRAVPEALCYMHPEDAIKYWVKQGELAWVESRRGKVKARVETRGRNRPSRGLVFVP
                     WFDEKVFINKVCADYTCPQSGQTDFKKCAVKI"
BASE COUNT          476 a          177 c          269 g          351 t
ORIGIN      
        1 catcaacata tcatgaaaat ccatagagat attgaagatg gaaatataaa atttgcttgg
       61 gtaaatgttt gtaatccata tcaagataca gccaatgcaa cacactgggt aaaagcagca
      121 agagaaatgg ataactttat cgtaacatct gacggatacc ctggaatttc tgcaaaagta
      181 tctgacctta ttctaccatc tgctatgatt tatgaaaaat ggggtggata tggaaatgct
      241 gaaagaagaa ctcaactttg gagacagcaa gttgttccag ttggagatgc tatgagtgat
      301 acatggcaat gggttgagct ttcaaaaaga tttacagtaa aagatgtttg gggagaacaa
      361 gctcttttaa gtacaaacaa tgaaactaaa cttcctagcc ttatagaagc agcaaaagct
      421 atgggataca atgaaaatac aactatgtat gagattttat ttgcaaatga tagagcaaaa
      481 tcatataaag cagaccaaaa agatgaaatt caagctggat atgataatac tgaagcattt
      541 ggtgatagta gaaatgtttt aggaagtgat ggaaaaccat ggaaaggtta tggattcttt
      601 ttacaaaaat atctatttga agagtatgca gattttggaa gaggacatgc ccacgattta
      661 gctgattttg acacttatca tagagttaga ggtcttaaat ggccagttgt agatgggaaa
      721 gagacatcat ggagatttaa tacaaaatat gatccatatg caaaaatggc aaatccaaat
      781 agtgattttg ctttctatgg aacattagca aaagagttgg ctcaaggtga cctgcttgga
      841 gttaaagatc aaactaaaaa atctctaaaa aataaagcta aaattttcgc acgtccatat
      901 atggatccac ctgaaatgcc agatgagcaa tatccagttt ggttaagtac tggaagagta
      961 ctagagcact ggcatagtgg aactatgact atgagagttc ctgaactata tcgtgcagtt
     1021 cctgaagcac tttgttatat gcaccctgaa gatgctataa aatactgggt aaaacaaggg
     1081 gaactagctt gggttgaatc aagaagagga aaagtaaaag caagagttga aacaagagga
     1141 agaaatagac cttcaagagg attagtattc gttccttggt ttgatgaaaa agtatttatt
     1201 aataaagttt gtgcagatta tacttgtcca caatctggtc aaactgactt taaaaaatgt
     1261 gcggtaaaaa ttt
//
LOCUS       DAOQAN010000467         1264 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_173473_length_1264_cov_1.994210, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000467 DAOQAN010000000
VERSION     DAOQAN010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_173473_length_1264_cov_1.994210"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            235..741
                     /locus_tag="PKO10_07015"
     CDS             235..741
                     /locus_tag="PKO10_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66934.1"
                     /translation="MILNISKIKTEALLLFCRDLINTYKDVKPKTKSDLDLYYEFETI
                     NSDILKQLSNILYEPKYYIDNQKNFRVKAILKCYNFISKELEKNLKQNEEFNPSLLYF
                     SILALWFKELNKESTSKEFIFFTLYPYSFIYDKFLIKMSDVDYKIMNIKMIELSEIIV
                     SKYDRLTL"
     gene            complement(774..1094)
                     /locus_tag="PKO10_07020"
     CDS             complement(774..1094)
                     /locus_tag="PKO10_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HNW66935.1"
                     /translation="MKLLKILAASTLALAIASTSSFADVQKGQKAFIKFLKEPCGMDG
                     AKFALKHTQEEWRKIKADGTAEAEIIKICPKVKAGDIKESLLEHVMDFSIEFASDSGN
                     VPSC"
BASE COUNT          490 a          169 c          144 g          461 t
ORIGIN      
        1 gtcttaaaat ctctagagac tcttttgagt cgtcagcttt tataatatct ccttcgatat
       61 ttaaagcttc agcagtctga aggtcataaa tttgaccatc atgtaataaa ccaatttgtt
      121 ccaatatttt cctttttttt caaagtctta taaaatgcaa ttattttatc ttaaaaaaac
      181 ttaaacaata taaatcatat agttatttta gaaatatttg gttacaattt atctatgatt
      241 ctaaatattt ctaaaataaa aacagaagca ttgttactct tttgtagaga cttaataaat
      301 acatacaaag atgtaaaacc aaaaacaaaa tctgatttgg atctttatta tgagtttgaa
      361 actattaata gtgatatatt aaaacagctt tcaaatattt tgtatgaacc aaaatattat
      421 atagataatc aaaagaactt tagagtaaaa gctattttaa aatgttataa ctttatatca
      481 aaagagcttg aaaaaaacct caagcaaaat gaagagttta atccatcact tctatatttt
      541 tcaatattag ctctttggtt taaagagtta aataaagagt caactagtaa agagtttatc
      601 tttttcacac tatatccata ttcatttatt tatgataagt ttttaataaa aatgagtgat
      661 gttgattata aaattatgaa tataaaaatg atagagttat ctgaaataat agtttcaaaa
      721 tatgatagat taacgttata aagatagatt taactatctt tataacttaa aaattagcaa
      781 gaaggaacat ttcctgaatc acttgcaaac tctatagaga aatccataac atgctcaagc
      841 agactctctt ttatatctcc agcttttact tttggacaaa tttttataat ctcagcttca
      901 gctgttccat ctgcttttat ttttctccac tcctcttgag tatgttttaa agcaaatttt
      961 gctccatcca tcccgcaagg ctcttttaag aatttaataa atgctttttg tcctttttga
     1021 acatctgcaa aagatgaagt tgatgcaata gccaaagcta aagttgaagc tgctaatatc
     1081 tttaatagtt tcatactatt ctcctaaatt aaatttagct acaattataa cctttagata
     1141 taaaaagtgt ctgaaattta gaaaaaaata taaataaaag agtaaaaatt gcaatacaat
     1201 attttaaaaa aaattgttga ttatcttagt aagaatgccc aaaatataaa gtttataaaa
     1261 agag
//
LOCUS       DAOQAN010000468         1256 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_175170_length_1256_cov_2.293089, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000468 DAOQAN010000000
VERSION     DAOQAN010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1256
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_175170_length_1256_cov_2.293089"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="PKO10_07025"
     CDS             <1..182
                     /locus_tag="PKO10_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66936.1"
                     /translation="FFIIENILNIKVKDKNNRQLSSKTIELKGASSNNFDDAKINLIQ
                     KLKKYEEQNSILPFE"
     gene            246..>1256
                     /locus_tag="PKO10_07030"
     CDS             246..>1256
                     /locus_tag="PKO10_07030"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3,4-dihydroxy-2-butanone
                     4-phosphate synthase/GTP cyclohydrolase II"
                     /protein_id="HNW66937.1"
                     /translation="MNAITRVKEAIEEIKKGNMVIMLDDEDRENEGDLVYSAALSTPE
                     LVNFMVTHAKGLVCVSVSSETAKRLELFPMVSNNTSSYETAFTVSVDDVNAATGISAI
                     ERDNTIKILANPIAKAVELVRPGHIFPLIAKDGGVLVRTGHTEGSVDLCKLAGLKGEA
                     VICEILKEDGTMARRDDLDIFAAKHNLKQIYISDLVEYRLANETLVCEVSSSNIEFFG
                     KEAIKKEFKDHLENIHTVILFGKEEDVNHVKFHTIRPDIKIFLHDNKLNSMLKTINFL
                     QSKGGVLIFLNNSNKNQELEKNYGIGAQILNSLKIKKIKLMTSGGKHSFVGLQGFGLE
                     ILE"
BASE COUNT          521 a          128 c          217 g          390 t
ORIGIN      
        1 gtttttttat tattgaaaat attttaaata ttaaagtaaa agataaaaat aatagacaat
       61 tatctagtaa aactattgaa ttaaaaggtg catcatcaaa taactttgat gatgcaaaga
      121 ttaatctaat tcaaaaacta aaaaaatatg aagagcaaaa tagtattttg ccatttgaat
      181 aagtagtcca tatacaaggg cttttttgat ataatccaca aaaaatataa tttaaaagag
      241 aaaatatgaa tgcaataaca agagtaaaag aagcaataga agagataaaa aaaggcaata
      301 tggtaataat gctagatgat gaagatagag aaaatgaggg agatttagta tattcggcag
      361 cattgagtac tccagagctt gtaaatttta tggtaactca tgccaaaggt ttggtttgtg
      421 tgagtgtatc aagtgaaaca gcaaaaaggc ttgaactttt tcctatggtt tcaaataata
      481 catcatcgta tgaaacagct tttacagttt cggttgatga tgtaaatgca gctactggaa
      541 ttagtgcaat tgaaagagat aatactatta agattttagc aaatccaata gcaaaagctg
      601 tagaacttgt aagaccagga catatatttc cacttattgc aaaagatggt ggagtattag
      661 ttcgaacagg tcacacagaa ggaagtgttg atttatgtaa gttagctgga ctaaaaggtg
      721 aagctgttat ttgtgaaatt ctaaaagaag atggaacaat ggcaagaaga gatgatttag
      781 atatttttgc tgctaaacac aatttaaaac agatttatat atcagattta gttgaatata
      841 gacttgcaaa tgaaacttta gtttgtgaag tttctagctc aaatatcgaa ttttttggta
      901 aagaagctat aaaaaaagag tttaaagatc atcttgaaaa tattcataca gttattcttt
      961 ttggaaaaga agaagatgta aatcatgtaa aatttcatac aataagacct gatattaaaa
     1021 tatttttaca tgataataaa ctaaactcta tgttaaaaac tataaacttt ttgcaaagta
     1081 aaggtggagt tttaatattt ttaaataact caaataaaaa tcaagaatta gaaaaaaact
     1141 atggaatagg ggctcaaata ctaaactctt taaaaattaa aaaaattaag ctaatgacaa
     1201 gtggaggaaa acactctttt gtaggacttc aaggttttgg tttagagatt ttagaa
//
LOCUS       DAOQAN010000469         1251 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_176272_length_1251_cov_2.312709, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000469 DAOQAN010000000
VERSION     DAOQAN010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_176272_length_1251_cov_2.312709"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /gene="rplE"
                     /locus_tag="PKO10_07035"
     CDS             complement(<1..224)
                     /gene="rplE"
                     /locus_tag="PKO10_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HNW66938.1"
                     /translation="MASRLLEKYKAEIKPVLEAEFPKNKTLTAKIDKVVISVGAGEAM
                     KDTKLIQNMQDTISLIAGQRAVKVIAKKSVA"
     gene            complement(228..464)
                     /locus_tag="PKO10_07040"
     CDS             complement(228..464)
                     /locus_tag="PKO10_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002847984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HNW66939.1"
                     /translation="MAIKLKIKKGDTVRIIAGDDKGKTGEVLQVLPKVNKVIVKDCKV
                     AKKTVKPDQEKNPDGGFINKEMPIDISNVAKVEG"
     gene            complement(464..832)
                     /gene="rplN"
                     /locus_tag="PKO10_07045"
     CDS             complement(464..832)
                     /gene="rplN"
                     /locus_tag="PKO10_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HNW66940.1"
                     /translation="MIQSFTRLNVADNTGAKEIMCIKVLGGSKRRYATVGDVIVASVK
                     KALPTGKIKKGQVVKAVIVRTHKEVQRENGSLIRFDDNAAVILDAKREPVGTRIFGPV
                     AREVRYSGFMKIVSLAPEVL"
     gene            complement(832..1083)
                     /gene="rpsQ"
                     /locus_tag="PKO10_07050"
     CDS             complement(832..1083)
                     /gene="rpsQ"
                     /locus_tag="PKO10_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HNW66941.1"
                     /translation="MTHKREIQGVVVKRSGDKTASVLVTRSVLHPKYHKTVKRFKKYL
                     IHDEKNELNVGDSVIAIECRPLSKTKSFRLKTILATGVK"
     gene            complement(1094..>1251)
                     /gene="rpmC"
                     /locus_tag="PKO10_07055"
     CDS             complement(1094..>1251)
                     /gene="rpmC"
                     /locus_tag="PKO10_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HNW66942.1"
                     /translation="ELQGLLKEKKVLLFELKAKLKTMQLTNTSELRVVKKDIAKIQTA
                     MTAAKAN"
BASE COUNT          364 a          231 c          144 g          512 t
ORIGIN      
        1 gctacagatt ttttagcaat aactttaact gctctttgac cagcaattaa agagattgta
       61 tcttgcatat tttggattaa tttagtatct ttcatagctt caccagcacc aacagagata
      121 acaactttat caattttagc tgttaaagtt ttattttttg gaaactctgc ttcaagaact
      181 ggcttgattt cagctttata tttttctaat aatcttgatg ccatctttta cccttccact
      241 tttgccacat ttgaaatatc aattggcatc tctttattta taaatccacc atctggattt
      301 ttttcttgat caggtttaac agttttttta gcaactttac aatcttttac gattactttg
      361 tttacttttg gtaatacttg taaaacttct ccagtttttc ctttatcatc accagcaatg
      421 attctaactg tatcaccttt ttttattttt aatttaatag ccattatagt acctccggtg
      481 caagtgaaac gattttcata aatcctgaat atctaacttc tctagcaact ggtccaaaaa
      541 ttcttgttcc aactggctct cttttagcat ctaaaataac agcagcattg tcatcaaatc
      601 tgattaatga accattttct ctttgaactt ctttatgagt tcttacaatt acagctttaa
      661 caacttgacc ttttttaatt tttccagttg gaagagcttt tttaacagaa gcaacaataa
      721 catcaccaac agttgcatat cttcttttag aaccacctaa aactttgata cacataatct
      781 ctttagcacc tgtgttatct gctacattta atcttgtaaa actttgaatc attatttaac
      841 tcctgtagca aggatcgttt ttaatctaaa agatttagtt ttagatagtg gtctacattc
      901 aatagcaata acactatcac caacatttaa ttcatttttt tcatcatgaa ttaaatattt
      961 tttaaatctt ttaactgttt tatgatactt tgggtgtaaa actgatcttg taactaaaac
     1021 agaagctgtt ttatcacctg atctttttac tactacacct tgaatctctc ttttatgtgt
     1081 catacttaga tccttagtta gcttttgcag cagtcatagc tgtttgaatt ttagcgatgt
     1141 cttttttaac aactcttaat tcagaagtat tagttaactg cattgttttt agcttagctt
     1201 ttaattcaaa aagaagcacc tttttctctt ttaataatcc ttgaagttca t
//
LOCUS       DAOQAN010000470         1243 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_178057_length_1243_cov_1.773569, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000470 DAOQAN010000000
VERSION     DAOQAN010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_178057_length_1243_cov_1.773569"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..113)
                     /locus_tag="PKO10_07060"
     CDS             complement(<1..113)
                     /locus_tag="PKO10_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HNW66943.1"
                     /translation="MGQKVNPIGLRLGINRNWESRWFPKFETMPANVAEDD"
     gene            complement(113..445)
                     /gene="rplV"
                     /locus_tag="PKO10_07065"
     CDS             complement(113..445)
                     /gene="rplV"
                     /locus_tag="PKO10_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HNW66944.1"
                     /translation="MARAILKFIRVSPIKARLIAREVQGMNAEYAIASLQFTPNKAAG
                     IISKVIASAVANSGLDSADAVITSAREDKGPVLKRFTPRARGSASPKHKPTAHIMIEV
                     AASTKGDK"
     gene            complement(448..723)
                     /gene="rpsS"
                     /locus_tag="PKO10_07070"
     CDS             complement(448..723)
                     /gene="rpsS"
                     /locus_tag="PKO10_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HNW66945.1"
                     /translation="MARSIKKGPFVDSHLLKKVVAANSSKDKKPIKTWSRRSTILPDM
                     IGLTFNVHNGRNFVPVNVTENHVGYKLGEFAPTRTFKGHKGSVQRKA"
     gene            complement(733..>1243)
                     /gene="rplB"
                     /locus_tag="PKO10_07075"
     CDS             complement(733..>1243)
                     /gene="rplB"
                     /locus_tag="PKO10_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014473689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HNW66946.1"
                     /translation="PAGLKVGDKVQSAISGLDILPGNAMQLVSIPVGTMVHNIELKPG
                     KGAQIARSAGGYAQIMGREDKYVILRLPSGEMRKILGVCMATIGVVGNGDYINMVIGK
                     AGRTRHLGIRPQTRGSAMNPIDHPHGGGEGKTNSGRHPVTPWGMPTKGYKTRKKKASD
                     KLIISRKKK"
BASE COUNT          340 a          262 c          175 g          466 t
ORIGIN      
        1 ttgtcatctt cagctacatt tgcaggcatt gtttcaaatt ttggaaacca tctagactcc
       61 caatttctat taatacctaa tcttaaacct attggattaa ctttttgacc cattacttgt
      121 ctccttttgt agatgcagct acttcaatca taatatgtgc agttggttta tgctttggtg
      181 aagctgaacc tcttgctctt ggagtaaatc ttttaagaac tggtcctttg tcttctctag
      241 cacttgtaat tactgcatca gctgaatcca atccagagtt tgcaacagca gaagctatta
      301 ctttagatat aattccagca gctttgtttg gagtaaattg taaagatgca attgcatact
      361 ctgcattcat cccttgaact tctctagcaa ttaatcttgc tttaatagga gaaactctaa
      421 taaattttaa tatcgctctt gccattatta cgcctttctt tgaacagaac ctttatggcc
      481 cttaaatgtt ctagttggtg caaactcacc taatttatac ccaacatggt tctctgtaac
      541 atttacagga acaaagtttc taccattatg tacattaaat gttaatccaa tcatatctgg
      601 aagaattgta gatcttcttg accatgtttt aattggtttt ttatcttttg aactattagc
      661 agccacaact ttttttagta agtgtgaatc tacaaaaggt ccttttttta ttgatcttgc
      721 catcttaaac ccttacttct tttttcttga aatgattagt ttatcactag cttttttctt
      781 tctagtttta taacctttag ttggcatacc ccatggagta acaggatgtc ttccagaatt
      841 cgttttacct tctcctcccc cgtgtgggtg atcaataggg ttcatcgcag atcctcttgt
      901 ttgaggtcta attcctaagt gtcttgttct accagcttta ccaattacca tatttatata
      961 atccccgttt ccaactacac caatagtagc catacaaact ccaaggattt ttctcatttc
     1021 accagatggt aatcttaaga taacatattt atcttctctt cccataattt gtgcatatcc
     1081 accagcactt ctagcaattt gagcaccttt acctggtttt aactcaatat tgtgaaccat
     1141 tgttccaaca ggaatactta ctagttgcat agcatttcct ggtaaaatat caagtccaga
     1201 aatagcagat tgaactttat ctcctacttt taaaccagca ggt
//
LOCUS       DAOQAN010000471         1239 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_178826_length_1239_cov_3.048142, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000471 DAOQAN010000000
VERSION     DAOQAN010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1239
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_178826_length_1239_cov_3.048142"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="PKO10_07080"
     CDS             <1..576
                     /locus_tag="PKO10_07080"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014475507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HNW66947.1"
                     /translation="QVLTDMIFKVNKNSRVFTLDTGRLHSETYRVLDATNLKYGVKIE
                     VFFPKFEDVENLYKTQGVNGHFESIDKRKNCCNIRKIEPLKRALKSVDIWITGLRASQ
                     SITRETMPIVEWDENFKVVKLNPLINWSEDDVWNYIKENKVPYNKLHDNGFPSIGCEP
                     CTRAIKDGEDIRAGRWWWENPEHKECGLHKK"
     gene            900..>1239
                     /locus_tag="PKO10_07085"
     CDS             900..>1239
                     /locus_tag="PKO10_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012148033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HNW66948.1"
                     /translation="MLDTKQLTHLKQLEAESIHIIREVVAEFDNPVMMYSVGKDSAVM
                     LHLALKAFAPAKLPFPLLHVDTLWKFKEMIAFRDKRAKEEGFELIVHTNPDGIAQGIG
                     PFTHGSAVHTD"
BASE COUNT          476 a          151 c          236 g          376 t
ORIGIN      
        1 caagttttaa cagatatgat atttaaagta aataaaaata gtagagtatt tactcttgat
       61 actggaagat tacatagcga aacttacaga gttttggatg caacaaattt aaaatatggt
      121 gtaaaaatag aagttttttt cccaaaattt gaagatgttg aaaacttata taaaactcaa
      181 ggtgtaaatg gtcactttga aagtattgat aagagaaaaa attgctgtaa tattagaaaa
      241 atagagcctt taaaaagagc tcttaagagt gttgatattt ggattacagg acttagagca
      301 tctcaaagta ttacaagaga aactatgcca atagttgagt gggatgaaaa ttttaaagtt
      361 gtaaaactaa atccacttat aaactggagc gaagatgatg tttggaatta tataaaagag
      421 aataaagttc catacaataa actacacgat aatggctttc caagtattgg ttgtgaacct
      481 tgtacaagag ctattaaaga tggtgaggat ataagagctg gaagatggtg gtgggagaat
      541 ccagagcata aagagtgcgg gcttcataag aaatagtaga agtttttcca aaaaggtatt
      601 ttatatactc cttttgaaaa tcaaaatata ttgccgtaag gcaattccac tgctttagca
      661 cgggacactt tgttttttta ggaaaaagat tttttatata aatttcgtta agaaaatgaa
      721 actgtttgaa gcaaacttta gtttgcaagt ttttcatttt tagaaattta ttgaaaaaat
      781 ccctaaaaag gcaaccgtgt tccgattttg catacttttt taaaaagtat gagaagaaaa
      841 aaaagagctg aggctaaagc ctcagttcca aagtacacaa attaaagagg agaaaataga
      901 tgttagatac aaaacaacta acacatttaa aacaactaga agctgaatca attcatataa
      961 ttagagaagt tgttgcagag tttgataatc ctgttatgat gtactctgtt ggaaaagatt
     1021 cggcagttat gttacattta gcactaaagg catttgctcc tgcaaaatta ccatttccac
     1081 ttttacatgt ggatacttta tggaaattta aagagatgat tgcttttaga gataaaagag
     1141 caaaagaaga aggttttgaa ctaatagtgc atacaaatcc ggatggaatt gctcaaggaa
     1201 ttggaccttt tactcacgga agtgcagtgc acactgata
//
LOCUS       DAOQAN010000472         1237 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_179267_length_1237_cov_3.404399, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000472 DAOQAN010000000
VERSION     DAOQAN010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_179267_length_1237_cov_3.404399"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..146)
                     /locus_tag="PKO10_07090"
     tRNA            complement(72..146)
                     /locus_tag="PKO10_07090"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(112..114),aa:Asn,seq:gtt)
     gene            complement(273..>1237)
                     /gene="hemE"
                     /locus_tag="PKO10_07095"
     CDS             complement(273..>1237)
                     /gene="hemE"
                     /locus_tag="PKO10_07095"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014474986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HNW66949.1"
                     /translation="GRYLKEYMEVRSKAGNFLNLCHNPKLAAEVTIQPLDIVGVDAAI
                     LFSDILVIPNEMGMHLEFLQGEGPVFKDPIKTEADLDKLIGGVEAANKLTYVYETIKL
                     LKQQLPEDKALIGFTGAPWTLATYMIEGQGTKTYNLCKKIMYSNPEFLHKILRKVTDV
                     VKFYLEKQIEAGADVVQIFDSWAAAIEPGRYDEFSWKYMVEIAEYIKERYPHIPVIMF
                     PKGVAAFIERGLVYGNFDVFGVDWGTPMALAKEKLGSKYVLQGNMEPCRLYSKEETTK
                     CVEAIQNVMKGEGHIFNLGHGILPDVPVENAIHFVKECQRVSKK"
BASE COUNT          405 a          233 c          148 g          451 t
ORIGIN      
        1 tagtttacaa agattactta taaacttcat acatatttta aagaactcta atttttaata
       61 aaattataaa atggctccgg cacctgggat cgaaccaggg accaaatgat taacagtcat
      121 ctactctacc gctgagctat gccggaatct ttctttatcg ttaatggggt ggaattatag
      181 taaaaataaa ttttattgtc aagagttttt gcaataaatt tataaattta ataaatttat
      241 tgcaaagcaa gtttaaaatt agtaatcatt tactactttt tacttactct ttgacactct
      301 tttacaaagt gaatagcatt ttcaactgga acatctggta atataccatg acctaagtta
      361 aatatatgcc cctcaccttt cataacattt tgaattgctt caacacattt tgtagtctct
      421 tctttagagt acaatctaca tggctccata tttccttgaa gtacatattt acttccaagt
      481 ttctctttag caagtgccat tggtgttccc caatctactc caaatacatc aaagtttcca
      541 taaacaagcc ctctttctat aaaagcagca actccttttg gaaacataat tacaggaata
      601 tgtggatatc tctcttttat atactctgca atttcaacca tatatttcca agagaactca
      661 tcataacgac caggttcaat tgcagctgcc caagaatcaa atatttgaac aacatctgcc
      721 cctgcttcta tttgtttttc taaataaaat ttaacaacat ctgttacttt tcttaaaatt
      781 ttatgtaaaa actctggatt tgaatacatt atttttttac aaagattata tgtttttgtt
      841 ccttgccctt caatcatata agttgctaat gtccaaggtg ctcctgtaaa accaattaaa
      901 gctttatctt caggcagttg ttgttttaaa agttttatag tttcataaac ataagttaat
      961 ttattagcag cttcaactcc tccaattaat ttatccaaat cagcttcagt tttaattgga
     1021 tctttaaata caggaccttc tccttgtaga aattcaagat gcattcccat ctcatttgga
     1081 ataactaaaa tatcactaaa taaaattgca gcatcaactc ctactatatc aagtggttga
     1141 atagtaactt cagcagcaag ttttggatta tgacaaagat ttaaaaaatt tccagctttt
     1201 gatcttactt ccatatactc ttttagatat ctccctg
//
LOCUS       DAOQAN010000473         1235 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_179747_length_1235_cov_2.820339, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000473 DAOQAN010000000
VERSION     DAOQAN010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_179747_length_1235_cov_2.820339"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..725
                     /locus_tag="PKO10_07100"
     CDS             <1..725
                     /locus_tag="PKO10_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HNW66950.1"
                     /translation="VYKEGAKFGESERVLRVNNLGSEDLKKDLALAKEIELDGILFSK
                     IDTKEDVLQAAKLIEEVNPNLTLMIMIETPLSVLNIQEICAASPKVEVVVVGSNKLAN
                     RLQIDIKRGSKAIVTYLAQIALAAKAYGKTVIDGPHFDVKDEFACEDSTKDAFNLGFD
                     GKSLVHPIQIEYINDIFTPKQSEVEDYEDMINKYEEANKHGKEVILHNNRLVDSSRIA
                     WARKMIKLYETYKALGQNLFGK"
     gene            735..>1235
                     /locus_tag="PKO10_07105"
     CDS             735..>1235
                     /locus_tag="PKO10_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013136790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HNW66951.1"
                     /translation="MSKISNKINFGNYFEDFSIGQKIVHPLPRTISEGDVSLYIAFTG
                     SRFSLHSSDVVAKEFGYEKRPIDDILMFHLTFGKSVQDISLNAIANLGYAEISFPNPV
                     FTGDTVSMTSKVIGLKENSNGKSGVVYVHSIGVNQNGNEVLNFKRWVMVHKKDHENLS
                     GINDVPT"
BASE COUNT          482 a          137 c          221 g          395 t
ORIGIN      
        1 aagtttacaa agaaggtgct aaatttggtg aaagtgaaag agtattaaga gtaaataatc
       61 taggaagcga agatttgaaa aaagatttag ctttggcaaa agagattgaa cttgatggta
      121 tcttattctc taaaattgat acaaaagagg atgtattaca agctgctaaa ttaattgaag
      181 aggtaaatcc aaatcttaca cttatgatta tgattgaaac tccattatct gttttaaata
      241 ttcaagaaat ttgtgcagca agtccaaaag ttgaagttgt tgttgttggt tcaaacaaat
      301 tagcaaatag acttcaaata gatataaaaa gaggttcaaa agctattgtt acatatttag
      361 ctcaaattgc acttgcagca aaagcttatg gaaaaactgt aatagatggt ccacattttg
      421 atgtaaaaga tgagtttgct tgtgaagatt ctacaaaaga tgcatttaat ttaggatttg
      481 atggaaaatc tcttgtacat ccaatacaaa ttgagtatat aaatgatata ttcactccaa
      541 aacaaagtga agttgaagat tatgaagata tgatcaacaa atatgaagag gcaaacaaac
      601 acggtaaaga ggttatttta cataacaata gattagttga ttcttcaaga attgcatggg
      661 ctagaaagat gattaaattg tatgaaactt acaaagcttt aggtcaaaat ctatttggaa
      721 aataaggaga taaattgtca aaaattagca ataaaataaa ctttggtaac tattttgaag
      781 attttagtat tggtcaaaaa atagttcatc cacttcctag aactataagt gagggtgatg
      841 tatctttata catcgctttt acaggaagta gattttcttt gcactcaagt gatgttgtag
      901 caaaagaatt tggatatgaa aaaagaccaa tcgatgatat tttaatgttt catttaacat
      961 ttggaaaatc tgttcaagat atctctttaa atgcaattgc aaatttagga tatgctgaaa
     1021 tatcttttcc taatccagtt tttacaggtg atactgtatc aatgacttca aaagttattg
     1081 gattaaaaga gaactcaaat ggtaaaagtg gagttgtata tgttcactct attggagtaa
     1141 atcaaaatgg aaatgaagtt ttaaatttta aaagatgggt tatggttcat aaaaaagacc
     1201 acgaaaactt aagtggaata aatgatgttc caact
//
LOCUS       DAOQAN010000474         1235 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_179773_length_1235_cov_2.533898, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000474 DAOQAN010000000
VERSION     DAOQAN010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_179773_length_1235_cov_2.533898"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="PKO10_07110"
     CDS             complement(<1..634)
                     /locus_tag="PKO10_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014474983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016730 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-thioredoxin reductase catalytic
                     domain-containing protein"
                     /protein_id="HNW66952.1"
                     /translation="MIKKIDINSLAFQNELENTKEFTKDVLKKYNFVFNPDDEVNLSV
                     QMGLARNMLIYGKRYCPCFMVVEDENENRLCPCVPALSNEIPKNGSCHCGIYCTKEKA
                     HELLLNIDTKEAIDTHFRGLTKKECEDLLSNDEINSIELEALLEARDEGAVNFCLVDT
                     REWMEWVNIRIKGTDFLVPTTSFYNSLEQINDKKDIPIILYCHSGSRSAYC"
     gene            complement(717..1082)
                     /locus_tag="PKO10_07115"
     CDS             complement(717..1082)
                     /locus_tag="PKO10_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66953.1"
                     /translation="MELGNIPKIDQFKDNKAVEMQQVKSTSNLAKTDEQRELKEIQKK
                     SIDQAKENSDVQKAQNESALKSKYEVVLSNTNFGFNSSSQDFFVRVERGNSENQYPTE
                     DMMRVKAQMMALQKADTES"
BASE COUNT          394 a          211 c          126 g          504 t
ORIGIN      
        1 gacaataagc acttctactt ccactatgac aatacaatat tataggtata tcttttttat
       61 catttatctg ctctaaagag ttataaaaac tagttgttgg aactaaaaaa tcagttcctt
      121 ttattctaat attaacccac tccatccact ctcttgtatc tactaaacaa aaatttacag
      181 caccctcatc tcttgcttct aaaagtgcct ctagttctat agaatttatc tcatcattgc
      241 taagtaaatc ttcacactct ttttttgtca aacctctaaa atgagtatct atagcctctt
      301 ttgtatcaat atttaaaagc aattcatgag ctttctcttt tgtacaataa attccacagt
      361 gacaacttcc attttttggt atctcattac tcaaagctgg tacacatgga caaagtctat
      421 tttcattttc atcttcaact accataaaac aagggcaata tctttttcca taaataagca
      481 tatttctagc taatcccatt tgtacagata aattaacttc atcatcagga ttaaaaacaa
      541 aattatattt ttttaataca tcttttgtaa actcttttgt attttcaagc tcattttgaa
      601 aagctaaaga gttaatatca atttttttta tcatttataa accttttaaa ataaggtaaa
      661 attataatga gtttttaagt agatttggct taaaaatatt ttttaagcca aaatttctaa
      721 gattcagtat ctgctttttg tagtgccatc atctgagctt ttactcgcat catatcttct
      781 gttggatatt gattttcaga atttcctctc tcaactctta caaaaaaatc ttgagagcta
      841 ctattaaatc caaaatttgt atttgaaagt accacttcat atttactctt taaagctgat
      901 tcattttgag ctttttgaac atcagagttt tcttttgctt gatctatact cttcttttgt
      961 atctctttta actctctttg ttcatcagtt ttagctaaat ttgaagtaga ttttacctgc
     1021 tgcatctcta cagctttatt atccttgaac tgatcaattt tcgggatatt tccaagttcc
     1081 ataaatactc cttttcttaa atttagctaa ttatactata tttttttaga aaaagcattt
     1141 tattttgtta taatatttca aactttatta gggatattta tgaatagcac ttttgattat
     1201 gaaaagttaa aactttttta tattggaaaa gaaaa
//
LOCUS       DAOQAN010000475         1234 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_179933_length_1234_cov_4.318066, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000475 DAOQAN010000000
VERSION     DAOQAN010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1234
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_179933_length_1234_cov_4.318066"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..924)
                     /gene="istA"
                     /locus_tag="PKO10_07120"
     CDS             complement(<1..924)
                     /gene="istA"
                     /locus_tag="PKO10_07120"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HNW66954.1"
                     /translation="MRKIVEVLRLHLKLNLSLRQSATLLKISLGTASNYVNRFKELNI
                     DIDKFISLNEYEQEKLFYPKNVCDIKPQTSKIMPDYIYIHNELKRKKQTKVTLSLLYE
                     EYKEANPNNYYGSTQFREYYSKFLNKINPSMKQVHISGEKVFVDYSGLTIPIVNQRTG
                     EITKAQIFVAVLGASGYTFVHATYSQKQRDFILSHTLAYDFFGGTPLIVVPDNLKSAV
                     IKNSKKDGIVLNESYAALAKHYNMAVSPARPYKPKDKSKVEQGVQGIQRWILAVLRHQ
                     TFFSVDELNDAISLLLDKYNAKIVKRFNKSRL"
BASE COUNT          411 a          182 c          162 g          479 t
ORIGIN      
        1 taatctactt ttattaaatc tttttacaat ttttgcgtta tatttatcta ataaaagtga
       61 tattgcatca tttagttcat caacactgaa gaaggtttga tgtcttaaaa cagctaatat
      121 ccatctttga attccttgaa caccttgttc tactttactc ttatctttgg gtttatatgg
      181 acgtgcagga cttactgcca tattataatg ctttgcaagt gcagcgtaac tttcattaag
      241 tacaattcca tctttttttg agttttttat aacagcactt ttaagattat caggtactac
      301 aattaatgga gtacctccaa agaaatcata agctaaagta tgagataaaa taaagtctct
      361 ttgtttttga gagtatgttg catgtacaaa tgtataacca cttgctccaa gaactgctac
      421 gaatatttga gctttagtaa tttcaccagt tctttgattt acaattggaa tagttaaacc
      481 actgtaatca acaaagactt tttcaccaga aatatgaact tgtttcattg atgggttaat
      541 tttattcaaa aactttgagt aatactctct aaattgtgtt gatccatagt agttattagg
      601 attagcttct ttatactctt cataaagtaa ggacaatgtt actttagtct gttttttcct
      661 ttttaattca ttatgaatat agatgtaatc tggcattatt tttgaagttt gtggttttat
      721 atcacaaaca tttttaggat aaaaaagttt ctcttgttca tactcattta atgaaataaa
      781 cttatctata tcaatattta actctttaaa gcgattaaca taattacttg cagttccaag
      841 ggatattttt aaaagagttg cactttgcct aagagacaga tttaacttta aatgaagtct
      901 taaaacttca actatttttc tcattgatac tttcctcaat tctactccta aaattttatt
      961 ttttagaagt atatttaatc tagaaaatat ccttatctta aataaaactg atttttcggt
     1021 tttatttaaa agtgttcaaa taatcactgt aaatagtgtt caaataaaag tgtaaacact
     1081 gttcaagtat ttctggaaac tgtgttcaag tgtttccgat ttttgcatat ttatagtaaa
     1141 aaggattttt catgataatc ggatatattc gagtcagcac agagcaacaa tcaacagcaa
     1201 atcaaaaaca taaaatccta gaatttgcac aatc
//
LOCUS       DAOQAN010000476         1230 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_180949_length_1230_cov_2.767660, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000476 DAOQAN010000000
VERSION     DAOQAN010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1230
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_180949_length_1230_cov_2.767660"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="PKO10_07125"
     CDS             <1..332
                     /locus_tag="PKO10_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014473724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HNW66955.1"
                     /translation="KYFDKGLSVSGFPCNQFLSQEPGTEDEIKEFCSLTYGVEFEMFS
                     KIDVNGENAHPLYKFLKESSKGVLGTEAIKWNFTKFLVDKNGKVVKRYAPSTKPSEIE
                     NDILNLL"
     gene            833..>1230
                     /locus_tag="PKO10_07130"
     CDS             833..>1230
                     /locus_tag="PKO10_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HNW66956.1"
                     /translation="MNDNIERVREVFTQKSTRVDTNKGEEFYNSLFEKSKARLKSLRE
                     QKPLRDIDMMEVIETEGVNRRDFMKWVSATTATLMLPPMFAPLVAEATELMNRVPVIW
                     IELQDCAGNTEALLRSSAPTVDDLLFDVLS"
BASE COUNT          493 a          129 c          185 g          423 t
ORIGIN      
        1 aaaaatattt tgataaagga ttatcagttt caggttttcc ttgtaatcag tttttatcac
       61 aagaaccagg aacagaagat gagattaaag agttttgctc attaacttat ggtgtagagt
      121 ttgaaatgtt tagtaaaatt gatgtaaatg gagagaatgc tcacccttta tataaatttt
      181 taaaagagag ttcaaaagga gttttaggaa ctgaggctat aaaatggaat tttacaaaat
      241 ttttagttga taaaaatggt aaagtggtaa aaagatatgc tcctagtaca aaaccaagtg
      301 agatagaaaa tgatatttta aacttacttt agaaatctaa aaaacagact caaataagcc
      361 catttttatt aagggcttat tttataaaga ttaaactttt acttttatac agaagtaaaa
      421 aaacttataa aagcaaataa acttcctatt agtagaaatg attcactcat ttatattctc
      481 caaattttaa atatttcaaa taatatcaat aaaatttaaa aatatctaaa aaaattgcaa
      541 tttgtaatat tttttgtctt attatatttt tataagcgaa ataatataaa gaaaaataat
      601 ctaaaaatag tgtaaaaaag taacaaattt aaataaaact gaaatagcca ttcacttata
      661 tgtttttaga acaaattaaa ctagaagagt gccttaaaat ggtaaatatt tttttacatt
      721 ttttaaaaaa gcaactagaa attaggcttt tactttataa ctatttatcc aaatataagt
      781 tttattacaa tattttgtta ttaatattta cttttaacta ggaggagtca atatgaatga
      841 taacattgag cgagtaagag aagtttttac tcaaaaatca actagagttg atacaaataa
      901 gggagaagag ttttataact cattatttga aaaatcaaaa gctagattaa aatctttaag
      961 agaacaaaaa cctcttagag atatagatat gatggaggtt attgaaactg aaggagtaaa
     1021 cagaagagat tttatgaagt gggttagtgc aacaactgca acacttatgc ttccacctat
     1081 gtttgctcct ttagttgcag aggctactga acttatgaat agggttccag taatttggat
     1141 agagttacaa gattgtgctg gaaatacaga agctctttta agaagttctg ctccaactgt
     1201 tgatgattta ttatttgatg ttttaagttt
//
LOCUS       DAOQAN010000477         1227 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_181776_length_1227_cov_1.786689, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000477 DAOQAN010000000
VERSION     DAOQAN010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1227
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_181776_length_1227_cov_1.786689"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            38..238
                     /locus_tag="PKO10_07135"
                     /pseudo
     CDS             38..238
                     /locus_tag="PKO10_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009295244.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
     gene            complement(299..>1227)
                     /locus_tag="PKO10_07140"
     CDS             complement(299..>1227)
                     /locus_tag="PKO10_07140"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNW66957.1"
                     /translation="SYFKYYPHFNWIIAAGVYIDDIENELNLKKDELKEEIRKQIIQN
                     IILFVIFLIIAIITLALISNKIYKILKNYKKQVIENEKELKVLNKSLEEMISNIAHQW
                     RQPLTELSSILMFIKLKFDTKTLDSKIMENKIEDATQVLEYMSHTIDDFRGFFSPKKE
                     KERFYLHELIENILLISSNMFKNNNIEIVCDIDKNIVLNSYLNELQQVILNILKNAKD
                     ALVENNIKSPYIKIEAKVDEKNIYLSITDNAGGIKTEPINKIFEAYFTTKPQGQGTGI
                     GLYMSKMILEKSMKGKLFVENIEDKARFTIKL"
BASE COUNT          400 a          163 c          136 g          528 t
ORIGIN      
        1 cttgattcta ttataaaaat gttgagaaac tcaggtggtt gtaaatatgg aaataggtat
       61 gttaacacca cctgttggac taaatctatt tgtcgcaagt ggaataactg gattatccat
      121 gggacaagtt ataaaagctt ctattcctat gactttagtt ttactttttg gattggtttt
      181 aattacttat ataccagcta tatctctatg gcttccaaat ttgatgtttg gttactaaaa
      241 agtttagaaa atattaaaaa ttgaaaatag aaaactgaaa ataaattttt cagttttctt
      301 ataattttat tgtaaaccta gctttatctt ctatattttc tacaaataat ttacctttca
      361 tacttttttc taaaatcatt tttgacatat aaagcccaat tccagtacct tggccttgag
      421 gtttagtggt aaaatatgct tcaaatattt tatttattgg ttctgtttta attccacctg
      481 cattatcagt tatagataaa tatatatttt tttcatcaac ttttgcttca attttgatat
      541 atggactctt tatattgttt tctactaaag catctttcgc attttttaaa atatttaaaa
      601 ttacttgttg aagttcattc aaatagctgt ttaaaactat atttttatca atatcacaca
      661 ctatttctat attattattt ttaaacatat ttgaactaat taataatata ttttctataa
      721 gttcatgcag ataaaatctc tctttctctt tttttggtga aaaaaatcct ctaaaatcat
      781 caatagtatg agacatgtat tctaaaactt gtgttgcatc ctctatttta ttctccataa
      841 ttttactatc tagagttttt gtatcgaatt ttaattttat aaacattagg atagaagata
      901 actctgttaa tggttgtctc cattgatgag ctatatttga aatcatctct tcaagagatt
      961 tatttaaaac ttttaactct ttttcgtttt caataacttg ttttttgtag ttttttaaaa
     1021 ttttataaat tttatttgaa ataagagcta gagttataat ggcaataatc aaaaaaatca
     1081 caaacagaat aatattttga ataatttgtt ttcttatctc ctcttttaac tcatcttttt
     1141 ttaagtttaa ctcattttct atatcatcta tataaactcc agctgctatt atccagttga
     1201 aatgtgggta atatttaaaa tatgata
//
LOCUS       DAOQAN010000478         1225 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_182273_length_1225_cov_1.694872, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000478 DAOQAN010000000
VERSION     DAOQAN010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1225
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_182273_length_1225_cov_1.694872"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            47..937
                     /gene="rfbA"
                     /locus_tag="PKO10_07145"
     CDS             47..937
                     /gene="rfbA"
                     /locus_tag="PKO10_07145"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014469198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HNW66958.1"
                     /translation="MKGIILAGGSGTRLYPITKGVSKQLVPIYDKPMIYYPLSVLMLA
                     GIKEVLIITTPQDQPSFINLLGDGKDLGMRFEYIIQPSPDGLAQAFILGEEFLDGDDA
                     CLVLGDNIFYGHGLTELLAKSVKNIKDENKATVFGYYVSDPERYGVAEFDESGNVISL
                     EEKPNNPKSNYAVVGLYFYPNDVVKKAKEVKPSDRGELEITTLNQAYLNENRLKVELM
                     GRRYAWLDTGTHESLLEASTFIQTIEHRQSLKVACLEEIAYEMGYISKEKLLELAEPL
                     KKNQYGQYLISRANQPRRMK"
     gene            940..>1225
                     /locus_tag="PKO10_07150"
     CDS             940..>1225
                     /locus_tag="PKO10_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014469197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HNW66959.1"
                     /translation="MTFTRTAIPDVVIIEPKVHGDSRGYFVETFRQDKLEEFLGYQIN
                     FCQDNESKSSKGVLRGLHYQLPPHAQTKLVRVISGRVLDVAVDIRKNSPTF"
BASE COUNT          474 a          160 c          230 g          361 t
ORIGIN      
        1 aaaaatgcaa ttaatgcaac atttaataaa gaaaaggagt aatgcaatga aaggaataat
       61 cctagcagga gggagtggga caagattgta cccgataaca aagggtgttt caaaacaatt
      121 agttccaatt tatgataaac ctatgatata ttatcctttg tctgttttaa tgctagcagg
      181 tataaaagaa gtattaatta ttacaactcc tcaagaccaa cctagtttta ttaatctttt
      241 aggtgatgga aaagatttgg gtatgagatt tgaatatata attcaaccaa gcccagatgg
      301 actagcacaa gcatttattt taggtgaaga gtttttggat ggtgatgatg catgtttagt
      361 ccttggtgat aatatttttt atggtcatgg attaacagaa cttttagcaa aaagtgtaaa
      421 aaatataaaa gatgaaaata aagcaactgt ttttggatat tatgtaagtg accctgagag
      481 atatggagtt gcagagtttg atgaatctgg aaatgtaatc tctttagaag aaaaaccaaa
      541 taacccaaaa tcaaattatg ccgttgtagg attgtatttt tatccaaatg atgtagtaaa
      601 aaaagcaaaa gaagtaaaac caagtgatcg tggtgaactt gaaatcacaa ctttaaatca
      661 agcttatcta aatgaaaata gattaaaagt tgaacttatg ggaagaagat acgcttggct
      721 tgatacagga acacatgaat cactgcttga agcaagtact tttatccaaa ctatagaaca
      781 tagacaaagt ctaaaagtag cctgtcttga agagatagca tatgaaatgg gatatataag
      841 taaagaaaaa cttcttgaac tagcagagcc tttaaagaaa aaccaatatg gtcaatattt
      901 aatctctaga gcaaatcaac caagaaggat gaaatagtta tgacatttac aagaacagct
      961 attcctgatg ttgtaattat tgaaccaaaa gttcatggag actcaagagg ttactttgtt
     1021 gaaactttta ggcaagacaa gctagaagag tttttaggat atcaaataaa tttttgtcaa
     1081 gataatgaat caaaaagctc taaaggagtt cttcgaggac ttcattatca acttccacct
     1141 catgcacaaa caaagctagt gcgagtaatt tctggaagag ttttagatgt tgcagttgat
     1201 ataagaaaaa attctcctac atttg
//
LOCUS       DAOQAN010000479         1224 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_182336_length_1224_cov_4.405475, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000479 DAOQAN010000000
VERSION     DAOQAN010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1224
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_182336_length_1224_cov_4.405475"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="PKO10_07155"
     CDS             <1..466
                     /locus_tag="PKO10_07155"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA dimethylallyltransferase"
                     /protein_id="HNW66960.1"
                     /translation="YMQKIEPNDKYRVEKAYSIYKQSGLTPSQYFLKNPKIALSPNLP
                     IFEILWEKDELINRISLRTKQMIKSGLIDETIYLEKKYTRAPNCMSSIGIIETLEYLD
                     GKIDKKSLEDKIIQNTLKLAKRQNTFNKGQFTNRVSNIIPSLNSEIIKYFSI"
     gene            547..750
                     /gene="rpmE"
                     /locus_tag="PKO10_07160"
     CDS             547..750
                     /gene="rpmE"
                     /locus_tag="PKO10_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014472884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HNW66961.1"
                     /translation="MKKDIHPDYKVCQVSCACGNSFESKSVTETLRVDICSNCHPFFT
                     GEQKLVDAAGRVEKFKAKYNMAK"
     gene            760..>1224
                     /gene="rsmI"
                     /locus_tag="PKO10_07165"
     CDS             760..>1224
                     /gene="rsmI"
                     /locus_tag="PKO10_07165"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HNW66962.1"
                     /translation="MLCLVPTPIGNLEDISKRSLEALLEAELIFCEDTRVTKKLLNLL
                     AEKYSLDFSQKEFKSFHSHNEKEILKSLTKDTFTKNVVYCSDAGMPCISDPGASLVDW
                     CIQNEVPYDVIPGANAILTAFAMSGFSSTEFTFFGFLDHKGTSRASKLEEVLN"
BASE COUNT          461 a          157 c          205 g          401 t
ORIGIN      
        1 ttatatgcaa aaaattgaac caaatgataa atatagagta gaaaaagcat attctattta
       61 taaacaaagc ggactaactc caagtcaata ttttttgaaa aacccaaaga ttgctctctc
      121 tccaaacctt ccaatttttg aaattttatg ggaaaaagat gagttaataa atagaatttc
      181 tttacgaaca aaacagatga taaaatctgg gcttatagat gagactatat atttagagaa
      241 aaaatataca agagctccaa attgtatgag tagcattgga ataatagaaa ctttagagta
      301 tcttgatgga aaaattgata aaaaaagttt agaagataaa attattcaaa ataccttaaa
      361 acttgcaaaa agacaaaata cttttaataa gggtcaattt accaatagag tttcaaatat
      421 aattcctagc ttaaattcag agattattaa gtatttttcg atataatcgc cgttcaaaag
      481 tctattaaag gacctgcaaa gacttgctct ttttttagac gtttaatatt ttaaaaggaa
      541 attggaatga aaaaagatat tcatccagat tacaaagtat gtcaagtttc ttgtgcatgc
      601 ggaaatagct ttgagtcaaa atcagttact gaaactttaa gagttgatat ttgttcaaat
      661 tgtcacccat tctttacagg agagcaaaaa ttagttgatg ctgctggaag agttgagaaa
      721 ttcaaagcta aatataatat ggcaaaatag tttattgcta tgttgtgctt agttccaaca
      781 ccaataggaa accttgaaga tatttcaaaa aggtctttag aggctctatt ggaggcggaa
      841 ctaatttttt gtgaagatac aagagtcaca aaaaaacttc taaatctttt ggctgaaaaa
      901 tattctttag atttttcaca aaaagagttc aaatcttttc actctcacaa tgaaaaagag
      961 atattaaaaa gtttaacaaa agatactttt actaaaaatg ttgtttattg tagtgatgct
     1021 ggtatgcctt gtattagtga ccctggtgca tctttggttg attggtgcat acaaaatgaa
     1081 gttccttatg atgtaatccc tggggcaaat gcaattttaa cagcttttgc tatgagtggt
     1141 tttagtagta cagagtttac attttttggt tttttagatc acaagggtac aagtcgtgct
     1201 tcaaaactag aagaggtttt aaat
//
LOCUS       DAOQAN010000480         1221 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_183126_length_1221_cov_2.628645, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000480 DAOQAN010000000
VERSION     DAOQAN010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_183126_length_1221_cov_2.628645"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="PKO10_07170"
     CDS             <1..439
                     /locus_tag="PKO10_07170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HNW66963.1"
                     /translation="TIKRLSMQISARNQIIGTIEKISLGAVNSEIQMRLKSGKSIVSI
                     ITNSSVENLGLAINDEVVAVIKSSNVLLSTQTNLKLSARNSLNGNIEEINIGSVNAEV
                     VVNIGNEDKIVAIVTINSIENMGLKVGASVDVIIKASDIMIGK"
     gene            450..1196
                     /gene="modA"
                     /locus_tag="PKO10_07175"
     CDS             450..1196
                     /gene="modA"
                     /locus_tag="PKO10_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012011931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNW66964.1"
                     /translation="MKKIVLALTFLCSSIFAGTINIAVAANVSYAINELIAQFNKTNP
                     DTKVEVVLGASGKFTTQIQNGAPFDIFLSADMKFPETLEKENLTATKPVIYAQGSVAM
                     LSAKPLDFSKGIALLANKDIEKIAIANPKTAPYGTAAIEALKNANILDKVESKFVYAE
                     SISQAVTYAMTAADVGFIAKSSLYDEKMSQYKENINWISIDPKLYTPIDQGIVILNRA
                     KDNKEAKAFYDFILSDNAKKIFIDFGYLVK"
BASE COUNT          468 a          139 c          215 g          399 t
ORIGIN      
        1 aactataaag aggctatcaa tgcaaataag tgcaagaaac caaataattg ggacgattga
       61 gaaaatttca ttgggtgcag ttaattctga aattcaaatg agactaaaaa gtggaaaatc
      121 tattgtctct attataacaa atagctctgt tgagaactta ggattagcta taaatgatga
      181 agttgttgct gttattaaat ctagcaatgt tttgttatca actcaaacta atttgaaatt
      241 aagtgctaga aatagcctaa atggaaatat tgaagagata aatattggtt cagttaatgc
      301 tgaagttgtt gtaaatattg gaaatgaaga caaaattgtt gctattgtga ctataaattc
      361 tatagaaaat atgggtttaa aagttggagc aagtgttgat gttattataa aagcatctga
      421 tattatgatt ggaaaataag gagaaaatta tgaaaaaaat tgttttggca cttacatttt
      481 tatgttcaag catatttgct ggtactataa atatagctgt tgcagcaaat gtttcttatg
      541 caataaatga gttaattgcc cagtttaata aaacaaatcc agatacaaaa gttgaagttg
      601 ttttaggggc tagtggaaag tttactacac aaattcaaaa tggagcgcca tttgatattt
      661 tcctaagtgc tgatatgaaa tttcctgaaa ctttagaaaa agagaactta actgctacaa
      721 aaccagttat ttatgctcaa ggaagtgttg caatgttaag tgcaaaacca ttagatttct
      781 caaaaggtat tgctttgctt gctaataaag atattgaaaa aattgctatt gcaaacccaa
      841 aaactgctcc ttatggaaca gctgctattg aagcacttaa aaatgcaaat attttagata
      901 aagttgaaag taaatttgtg tatgctgaat ctatatctca agcagtaact tatgctatga
      961 ctgctgctga tgttggattt atagcaaaat catctttata tgatgaaaag atgtctcaat
     1021 acaaagagaa tattaactgg ataagtatag atccaaaact ttacactcca attgatcaag
     1081 gaattgttat tttaaataga gcaaaagata acaaagaagc aaaagcattt tatgacttta
     1141 ttttgagtga taatgcaaaa aaaatcttta ttgattttgg ttatttagta aaataaagag
     1201 tctattatga ttgcaagagt a
//
LOCUS       DAOQAN010000481         1219 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_183573_length_1219_cov_2.829897, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000481 DAOQAN010000000
VERSION     DAOQAN010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1219
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_183573_length_1219_cov_2.829897"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..>1219)
                     /locus_tag="PKO10_07180"
     CDS             complement(110..>1219)
                     /locus_tag="PKO10_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014469300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RecN"
                     /protein_id="HNW66965.1"
                     /translation="KNIKIDFDNKYKEFIDIEKRLSKIKDDEKNLEDLKEFAKFEIAK
                     IEEINPKENEYEELNSIKKALAKKEKIEVASKKASIIFENARAVNELLEILEVDSSFF
                     DEAINEFTNILEKANDTLSELEDINIEETLNRIEKISSLLKRFGTIKECLEYKEQKQK
                     ELENYNNISYEKDELEQKYNQLNEIILNLAYKISNYREKSIGILEKSVNYYLDFLYLN
                     SAKITLTNKKLDYSGVDFVEFTLNGVAISTISSGEYNRLRLALLSSISEFDIADNGVL
                     FLDEIDANLSGKESDAISKVLIKLSKNYQIFAISHQIQLASQANQHFLVEKIDGNSVV
                     TILDSTQRAKELARMISGENVTNEALDFVKNLLKS"
BASE COUNT          374 a          199 c           98 g          548 t
ORIGIN      
        1 ttgataatat taataatttt gcttttattg atatattttt tgacattttg tagctccaat
       61 gcaatgaaat gccattattg tatcattatt taaaaaaaat ctatattttt tagctcttta
      121 ataaattttt tacaaaatct aaagcctcat ttgtaacatt ttctccactt atcattcttg
      181 ctagctcttt tgctctttga gtactatcta aaattgtaac aactgagtta ccatctatct
      241 tttctaccaa aaagtgttga tttgcctgtg aagccaactg tatttgatga gaaattgcaa
      301 aaatttgata atttttactt agttttataa ggacttttga tattgcatca ctctcttttc
      361 cacttaagtt tgcatctatc tcatctaaaa aaagtactcc attatctgct atatcaaact
      421 cactaattga acttaaaagt gctaatctta acctattata ttcacctgag ctaatagttg
      481 atatagcaac cccatttaat gtaaactcta caaaatcaac accactataa tctaattttt
      541 tatttgttag agtaattttt gcactattta aatataaaaa atctaaataa tagttaacac
      601 ttttttctaa aattcctata cttttttctc tatagtttga tattttatat gctaagttta
      661 atattatctc atttagctga ttatactttt gctcaagctc atctttttca taactaatat
      721 tattgtaatt ttcaagctct ttttgtttct gctctttata ttctaaacac tcttttatgg
      781 taccaaatct ttttaataaa gaagatattt tctctattct atttaaagtc tcttctatat
      841 ttatatcttc aagttcacta agtgtatcat ttgctttttc taaaatattt gtaaattcat
      901 ttattgcttc atcaaaaaaa cttgaatcca cttctaaaat ctcaagtaac tcattaacag
      961 ctcttgcatt ttcaaaaatg attgatgctt ttttactagc aacttcaatt ttctcttttt
     1021 ttgccaaagc tttttttata ctatttaact cttcatactc attctctttt ggattaatct
     1081 cttctatttt tgctatttcg aattttgcaa actcttttaa atcttcaaga tttttttcat
     1141 catcttttat cttagataat cttttttcaa tatctataaa ttctttatat ttattatcaa
     1201 aatctatctt gatattttt
//
LOCUS       DAOQAN010000482         1217 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_184125_length_1217_cov_2.648881, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000482 DAOQAN010000000
VERSION     DAOQAN010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1217
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_184125_length_1217_cov_2.648881"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1217)
                     /locus_tag="PKO10_07185"
     CDS             complement(<1..>1217)
                     /locus_tag="PKO10_07185"
                     /inference="COORDINATES: protein motif:HMM:NF019874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HNW66966.1"
                     /translation="LKKFETTITKEKESLIQHQFEVAQTLIKTIIEKEQNIELAKQKV
                     IELLTGVRYLDDKSGYFFAYEQRGDDYYFAFHPANPKLNNTKTNIKSPDAKGYAFRED
                     LIKFSKEQKYITYHYENPSTKEVVLKMASSIFIPEFNWVLVTGIYADDIEKDINELQE
                     EMNRDINILFWIAICVTVLLNIVLISIIVPSINKIILKPLNIFQTTLESFFKYLNNES
                     NKVDRIKNYSKDEIGHMSKILDQNIEIARKEIDDNNLFIETTIDILEKFQKGDLSQRL
                     NIEVDDINLSKLKSVMNQMAQELEKNIVNVLKVIDEYSKYDYVNRVDTTKISNHILKL
                     ANGVNILGDSITKMLIENKKNGETLSESSNILLSNVEKLNESSTSTAANLEETAASLE
                     EMTSNLRSSTQNV"
BASE COUNT          396 a          172 c          116 g          533 t
ORIGIN      
        1 tacattttga gtactacttc ttaaatttga agtcatctct tctaaagaag cagctgtttc
       61 ttcaagattt gcagctgtac tagtagaact ttcatttaat ttttcaacat tactcaaaag
      121 tatatttgaa ctttcagata aagtttctcc atttttttta ttttcaatta acatttttgt
      181 tattgaatct cccaaaatat taacaccatt tgcaagtttt aatatatgat ttgaaatttt
      241 tgttgtatca actctattta cataatcata tttactatat tcatctatta cttttaaaac
      301 attcacaata ttcttttcta attcttgtgc catttgattc ataacagatt ttaattttga
      361 tagattaata tcatcaactt ctatatttaa tctttgagat aaatctcctt tttggaattt
      421 ttctaaaata tctattgtag tttcaataaa aagattgtta tcatctatct cttttctagc
      481 aatctcaata ttttgatcaa gtatttttga catatgacct atttcatctt ttgaataatt
      541 cttgattcta tcaactttat tgctttcatt atttaaatat ttaaaaaatg actctaaagt
      601 tgtttgaaat atatttagtg gttttaaaat aattttattt atagatggaa caattataga
      661 aatcaaaaca atatttaata aaactgtaac acaaatagct atccaaaaaa gtatatttat
      721 atctctattc atctcttctt gtaattcatt tatatctttt tcaatatcat ctgcatatat
      781 tccagttact aaaacccagt taaattctgg aataaaaata gatgaagcca tttttaacac
      841 aacctctttt gttgatggat tttcataatg ataagttata tatttttgct cttttgaaaa
      901 tttaattaaa tcttctctaa aagcataacc ttttgcatca ggagacttta tatttgtttt
      961 tgtattattt aattttggat ttgctggatg aaaagcaaaa tagtaatcat cacctctttg
     1021 ctcataagca aaaaaatatc cacttttatc atctaaatat ctaacgcctg ttaaaagctc
     1081 tataactttt tgttttgcta actctatatt ttgttctttt tcaataattg tttttataag
     1141 tgtttgcgca acttcaaatt gatgttgtat taaggactct ttctcttttg taatagtagt
     1201 ttcaaacttt tttaatg
//
LOCUS       DAOQAN010000483         1213 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_185052_length_1213_cov_2.537997, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000483 DAOQAN010000000
VERSION     DAOQAN010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_185052_length_1213_cov_2.537997"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            238..>1213
                     /gene="eno"
                     /locus_tag="PKO10_07190"
     CDS             238..>1213
                     /gene="eno"
                     /locus_tag="PKO10_07190"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012148084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNW66967.1"
                     /translation="MVFIDNVYADEVMDSRGNPTVRATVILSDGTKASAIVPSGASTG
                     KREALELRDADSRFLGKGVLKAVENVNTRIADELIGQSPFNQAEVDAIMKELDGTHNY
                     SNLGANAVLGVSMAVARAAATSLNMPLYRYLGGANAMTMPVPMFNIINGGEHANNSVD
                     FQEYMIMPTGFENFNEGLRAVAEIYQHLKKIIDAMGESTAVGDEGGFAPNLKSNEEPI
                     SVIMSAIEKAGYKAGEQISIALDVAASELIDEKTKKYVLKGENRELTSAQLVDYYADL
                     CSKYPIVSIEDGLSEDDWAGWKILTEKLGSKVQLVGDDLFVTNASIVAE"
BASE COUNT          425 a          149 c          233 g          406 t
ORIGIN      
        1 aggtaagctt ttttatttta cctcttgttt atacaagctt ttttatatct acattctaca
       61 cttaatattc aggttttaca tttattttct agtaaaagtt attttttata ttaagttaat
      121 tgaaaataat catattatct cttttttcat ttttaataaa ctatattata ataaaagcta
      181 aagccaaatt ttttttagat ataatcttgc taaaatattc aaaactagga gacccaagtg
      241 gtatttattg ataatgttta tgctgatgaa gtgatggatt caagaggaaa tccaactgtt
      301 agagcaactg taattttaag tgatggtacg aaagcgagtg ctatagttcc aagtggtgca
      361 agcacaggaa aaagagaagc tttggaatta agagatgcag atagcagatt tttaggtaaa
      421 ggtgttttaa aagctgttga aaatgtaaat actagaattg ctgatgagct aattggacaa
      481 agtccattta atcaagcaga agttgatgca attatgaaag aactagatgg aacacataac
      541 tactctaatc ttggagcaaa tgctgtttta ggtgtttcta tggcagtggc acgagcagct
      601 gcaacttcac ttaatatgcc actttataga taccttggtg gagcaaatgc tatgactatg
      661 cctgttccta tgtttaatat tataaatggt ggagagcatg caaataactc tgtagatttc
      721 caagagtata tgattatgcc aacaggtttt gaaaacttca acgaaggttt aagagcagtt
      781 gcagagattt atcaacacct taaaaaaatt attgatgcaa tgggtgaaag tacagcagtt
      841 ggtgatgagg gtggatttgc tccaaattta aaatcaaatg aagaaccaat ttcagttatt
      901 atgagtgcaa ttgaaaaagc tggatataaa gctggagagc aaatctctat tgctcttgat
      961 gttgcagctt ctgaattaat cgatgaaaaa acaaaaaaat atgttttaaa aggtgaaaat
     1021 agagaattaa cttcagcaca gcttgttgat tattatgctg atttatgttc taaatatcca
     1081 attgtatcaa ttgaagatgg tttaagtgaa gatgattggg caggatggaa aatcctaact
     1141 gaaaaattag gttcaaaagt tcaattagtt ggagatgatt tatttgttac aaatgcttca
     1201 atagttgctg agg
//
LOCUS       DAOQAN010000484         1213 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_185143_length_1213_cov_1.856649, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000484 DAOQAN010000000
VERSION     DAOQAN010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_185143_length_1213_cov_1.856649"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1213
                     /gene="bamA"
                     /locus_tag="PKO10_07195"
     CDS             <1..>1213
                     /gene="bamA"
                     /locus_tag="PKO10_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HNW66968.1"
                     /translation="EETLGMRTGDSLDSNKLNEALIKFYSYGYFDDIVIDNDNGNIKI
                     IFKEKPSIANVDIKGYKTRSEDTDAIKKVLKLNKGSMYSEKRVKEAKEQLLGMLSSEG
                     FINSVVEVETEKLNDSSIKLTFNVNKGDEIIIREAKYHGASELDGSDFKKVTANKEKE
                     FASWWFGQSDGEMKIDQLKYDARRINDLYFEKGYLDADVKEPFLDIDFASNQAKLDFF
                     VKEGEKYTTNDIKIFLDSSIVDPEEIYSDLKLKVDRTFNIKKLRDDQEYIRTLVADKG
                     YAYAEVKFDLKKNEAEHRVDVIFSVVPGQQVYINDVKISGNARTLDRVVRRDVYLAPG
                     DMYNLTDLKDAKSKLKRSSFFEDVQIEEKRISEDKMDLSVKVTEAPTGSIMLGGGYGS
                     YDKLMINGSVSD"
BASE COUNT          497 a          106 c          239 g          371 t
ORIGIN      
        1 agaagaaact ttgggaatga gaactggtga tagtttagat agtaacaagc taaatgaagc
       61 attaataaaa ttttatagct atggttactt tgatgatata gtaatagata atgataatgg
      121 aaatatcaaa attatcttta aagagaaacc ttcaattgca aatgttgata taaaaggtta
      181 taaaactaga agtgaagata cagatgctat taaaaaagta ttaaagctaa acaaaggttc
      241 tatgtatagc gaaaaaagag ttaaagaagc aaaagaacaa cttttaggaa tgttatctag
      301 tgaagggttt ataaactctg ttgttgaagt tgaaacagag aaattaaatg attcttctat
      361 aaaacttact tttaatgtaa ataaaggtga tgagattatc ataagagaag caaaatatca
      421 cggtgctagt gaactagatg gaagtgactt taaaaaagtt actgcaaata aagagaaaga
      481 gtttgcttct tggtggtttg gtcaaagtga tggtgaaatg aaaattgatc aactaaaata
      541 tgatgcaaga agaataaatg acctatattt tgaaaaaggt tacttagatg cagatgtaaa
      601 agagccattt ttagatatag attttgcttc aaatcaagca aaacttgact tctttgttaa
      661 agagggtgaa aaatatacta caaatgatat aaaaatcttt ttggactctt caattgtaga
      721 tcctgaagag atttattcag atttaaaact aaaagttgat agaacattta atattaaaaa
      781 attaagagat gatcaagagt atattagaac tttggttgct gataagggtt atgcctatgc
      841 agaagttaaa tttgatttaa agaaaaatga agctgagcat agagttgatg ttatttttag
      901 tgttgttcca gggcaacaag tatatataaa tgatgtaaaa atatctggaa atgcaagaac
      961 tcttgataga gttgtaagaa gagatgtata tttagcacct ggtgatatgt ataatcttac
     1021 agatttaaaa gatgcaaaaa gtaagttaaa aagatctagc ttctttgaag atgttcaaat
     1081 tgaagagaaa agaataagtg aagataaaat ggatttatct gtaaaagtaa cagaagcacc
     1141 aactggaagt attatgctag gtgggggata tggttcttat gataaattaa tgataaacgg
     1201 atctgtaagt gat
//
LOCUS       DAOQAN010000485         1209 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_186001_length_1209_cov_2.341421, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000485 DAOQAN010000000
VERSION     DAOQAN010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1209
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_186001_length_1209_cov_2.341421"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..>1209)
                     /locus_tag="PKO10_07200"
     CDS             complement(113..>1209)
                     /locus_tag="PKO10_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNW66969.1"
                     /translation="RLAEDYLYNLGKISNIHQEELEDKTKAKDIIISTLSNAYNQPSK
                     NICLTPSGMNAMYCVLKGIKNIQAKNGRTILVQLGWLYLDTMNIVNHYFEESKIFYDI
                     TNLDNLENFLKNNGSKVSAIITEIPTNPLVQTVDLEKLKNLCDSYNIPLVIDSTFATP
                     YNLNLNSYADIYVESLTKFACGNADVLMGAIILNKNSKISHISQEFFKHCDEPYIKDI
                     QRLALEIQGYKTRVKKISSNTKKLVEYFQKSSYIDEIFYCLSPKYKENYEKLVIDENS
                     LCGVVSITFKKDFQKVYDNLNFPKGPSLGTEFTLLMPYTYLAHYDLIVSKEGKEFLEK
                     INLPINLLRISVGVENIDEIKKEFEKLNTI"
BASE COUNT          408 a          160 c          136 g          505 t
ORIGIN      
        1 tcattttcac cagttaaagt atttgcaaca ctgtgaattg catcataaat aagtattgaa
       61 atattattaa tattgttttt atatgcttgt tttatcatct attttttaaa ccttaaatag
      121 tatttaattt ctcaaactct ttttttatct catctatatt ttctactcca acagatattc
      181 taagaagatt tataggaaga tttatttttt ctaaaaactc tttaccctct ttacttacta
      241 ttaaatcata gtgtgctaaa tatgtataag gcattaaaag tgtaaattca gttccaaggc
      301 ttggaccttt tggaaagttt aaattatcat aaactttttg aaaatctttt ttaaaggtga
      361 ttgatactac tccacataga ctattttcat caatcacaag tttttcataa ttctctttat
      421 attttggact taaacagtaa aaaatttcat ctatataaga tgatttttga aaatattcga
      481 ctaatttttt tgtatttgaa gatatttttt taactctagt tttataacct tgtatctcta
      541 aagccaatct ttgaatatct tttatatatg gttcatcaca gtgtttaaaa aactcttgtg
      601 aaatatgtga gatttttgag tttttattta aaataattgc acccattaaa acatctgcat
      661 ttccacaagc aaattttgtc aatgattcaa cataaatatc agcataagag tttaaattta
      721 gattatatgg tgttgcaaaa gtagagtcta taaccaaagg aatattataa ctatcacaaa
      781 gatttttaag tttttctaaa tcaacagtct gtacaagtgg atttgtagga atttctgtaa
      841 tgattgctga aacttttgat ccattatttt ttaaaaaatt ctccaagtta tcaagatttg
      901 taatatcata aaatatttta ctctcttcaa aatagtgatt tacaatattc atagtatcaa
      961 gatataacca tcctagttga actaaaatag ttcttccatt ttttgcttga atatttttaa
     1021 ttccttttaa aacacaatac attgcattca ttcctgaagg agttaggcaa atattttttg
     1081 atggttgatt ataggcattt gataaagttg aaattataat atcttttgct tttgttttat
     1141 cttcaagctc ttcttgatga atatttgaaa tttttccaag attatataaa taatcttcag
     1201 ctaatcttg
//
LOCUS       DAOQAN010000486         1209 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_186028_length_1209_cov_2.164645, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000486 DAOQAN010000000
VERSION     DAOQAN010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1209
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_186028_length_1209_cov_2.164645"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            162..>1209
                     /locus_tag="PKO10_07205"
     CDS             162..>1209
                     /locus_tag="PKO10_07205"
                     /inference="COORDINATES: protein motif:HMM:NF033923.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0046930 - pore complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Opr family porin"
                     /protein_id="HNW66970.1"
                     /translation="MKKLSLVVSTIMLSSTMFAASAFDKEVDAPLKKDNIENANSIDE
                     AFKNGKIEGSIALFGLTQDNKDQSIKRDFAFGNGNLTLGYNTASFYGFSLGTQVKGNI
                     KLGEKHKDDRKNEGPFENNALITQAYLQYSLNEMLLIKAGRYEGELEWLTDYQQGAIA
                     EITAIPDTTVSLAFSNRKAESGIDTSEDFHKPNDLNKGIYVVDIKNESLEGLEINPYY
                     YQIPDAVKFYGLKTSYNLEHFGAIAQYAKSSLTNEYKTNNSLENGYVAHFELNGNFED
                     LTSAVGLIKTSKKGGADIITSYGDSISPFEDGDKVYTLDARTIYGSLGYSIYDFDFGA
                     LYGITRYDASSSGLK"
BASE COUNT          473 a          126 c          203 g          407 t
ORIGIN      
        1 tttgattttt ctatttaaaa tagtagcaaa aatctactta ataaaaattt gttaattatt
       61 aataaaagat taataaagta ttaataaaaa aactgtatta ttccaaaatc taaaaataat
      121 gataacaagt atcatttaga ttattaatta aaggagtatt tatgaaaaaa ttaagtttag
      181 ttgtttcaac tattatgtta tcaagtacaa tgtttgctgc ttcagctttt gacaaagaag
      241 ttgatgctcc tttaaagaaa gataatatag aaaatgcaaa ctctatagat gaagctttca
      301 agaatggtaa aatcgaggga agcatagcac tatttggatt aactcaagat aataaagatc
      361 aatctattaa aagagatttt gcatttggta atggtaattt aactttagga tataacactg
      421 ctagttttta tggttttagt ttaggaactc aagtaaaagg aaatattaaa cttggtgaaa
      481 aacacaaaga tgatagaaaa aacgaaggac cttttgaaaa caatgcttta ataactcaag
      541 catatttaca atactcttta aatgagatgt tattaattaa agcaggtaga tatgaaggtg
      601 aattagagtg gcttacagat tatcaacaag gagctattgc cgagattaca gcaattcctg
      661 atactactgt atctttagca ttttcaaata gaaaagctga atcaggaatt gatacaagtg
      721 aagactttca taaaccaaat gatttaaata aaggtattta tgtagttgat attaaaaatg
      781 agagtttaga gggattggaa ataaatcctt actactacca aattccagat gcagtaaaat
      841 tctatggttt aaaaacttct tacaatttag aacattttgg agctattgca caatatgcaa
      901 agagtagctt aactaatgaa tataaaacta ataatagttt agaaaatggt tatgtagcac
      961 attttgagtt aaatggtaat tttgaagatt taacttcagc agttggttta ataaaaacaa
     1021 gtaaaaaagg tggagctgat attataactt cttatggaga tagtatttca ccatttgaag
     1081 atggagataa agtgtatact ttagatgcta gaactattta tggctctttg ggttattcaa
     1141 tttatgattt tgactttggt gctttgtatg gaattacaag atatgatgcg tcaagctctg
     1201 gattgaaag
//
LOCUS       DAOQAN010000487         1209 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_186035_length_1209_cov_2.110919, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000487 DAOQAN010000000
VERSION     DAOQAN010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1209
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_186035_length_1209_cov_2.110919"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1209
                     /locus_tag="PKO10_07210"
     CDS             <1..>1209
                     /locus_tag="PKO10_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNW66971.1"
                     /translation="VGHCYRCKNIVEPYISKQWFVKKEVAKKSIEKTYAGEAKFHPSH
                     WLNSYRAWMDELRDWCISRQLWWGQRIPVFYCDSCNHQWATKEDEPKMCPKCGKEHIH
                     QDPDVLDTWFSSALWAFSPLGWGNNGQMQKTFGANDLKDFYPNSLLITGFDIMFFWVA
                     RMMMMGEHFMGQLPFKDIYMHTLVRDEHGAKMSKSKGNVIDPLDMVETHSADIIRFTL
                     AYLAIQGRDIKLGEKNLEQFRNFTNKLYNASNFLTLNVDTFPDLKDIEIKTPLGLYMQ
                     SKLSHAVDELRNSLDSYKFNEAASTLYRFVWMEFCDWGIEYSKASKESIVELGSLFKE
                     TLKMVSPFMPFISDYLYHKLSGTTLENGDSLMIMNFPKDIKKDENIENMFSVIEEAII
                     AIRRAKTLID"
BASE COUNT          443 a          155 c          229 g          382 t
ORIGIN      
        1 aagtaggaca ctgttataga tgtaaaaata ttgttgagcc atatatttca aaacaatggt
       61 ttgtaaaaaa agaggttgct aaaaaatcaa ttgaaaaaac ttatgcaggt gaagcaaaat
      121 ttcatccaag tcactggtta aactcatata gagcttggat ggatgagtta agagattggt
      181 gtatttcaag acaactttgg tggggacaga gaataccagt attctattgt gatagctgta
      241 atcaccaatg ggctacaaaa gaggatgagc caaagatgtg tccaaaatgc ggtaaagagc
      301 atattcatca agacccagat gttttagata cttggtttag tagtgcttta tgggcatttt
      361 caccacttgg ttggggaaat aatggtcaaa tgcaaaaaac atttggtgca aacgatttaa
      421 aagattttta tccaaactca cttttaataa ctggatttga tattatgttc ttctgggttg
      481 ctagaatgat gatgatgggt gaacacttta tgggacaact tccatttaaa gatatatata
      541 tgcacacatt agttcgagat gagcatggtg caaaaatgag taaatcaaaa ggaaatgtaa
      601 tagatcctct tgatatggtt gaaactcata gtgctgatat tattagattt actttagcat
      661 atcttgcaat tcaaggaaga gatataaaac ttggtgaaaa aaatcttgag cagtttagaa
      721 actttacaaa taaactttac aatgcttcaa actttttaac actaaatgta gatacattcc
      781 ctgatttaaa agatattgaa atcaaaacac cacttggact ttatatgcaa agcaaactta
      841 gccatgctgt tgatgagctt agaaattcac ttgatagtta taagtttaat gaagcagcaa
      901 gtacacttta tagatttgtt tggatggagt tttgtgattg gggaattgag tactcaaaag
      961 cttctaaaga gtctattgta gaactaggta gcttatttaa agagacatta aaaatggtta
     1021 gtccatttat gcctttcatt tcagactatt tatatcataa attaagtgga acaacacttg
     1081 aaaatggtga ttcattaatg attatgaact tcccaaaaga tattaaaaaa gatgaaaata
     1141 ttgaaaatat gttctctgta attgaagagg cgattattgc tatacgaaga gcaaaaactc
     1201 taattgata
//
LOCUS       DAOQAN010000488         1207 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_186418_length_1207_cov_3.108507, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000488 DAOQAN010000000
VERSION     DAOQAN010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_186418_length_1207_cov_3.108507"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1207)
                     /locus_tag="PKO10_07215"
     CDS             complement(<1..>1207)
                     /locus_tag="PKO10_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014474456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNW66972.1"
                     /translation="LLNKPDFFVNNINEFFGSGNAFYYVAIVTAVVICLRAFHFIFGV
                     INTKIFTKISKIVIFDIRVKLLNHLKKVNMNEYESLGSGKISANLITDVNTLDSFIVN
                     VSSKLITSSLTLLAVGFVIIKIDFVLGLMILLTQPTIAIISRRIAKKTGELKKEENAT
                     IEAFQNNINETLDLFSQIKASNKEENFFDTSIKKAEDIKRASNEFNYKSVAYEKFSYT
                     IFLFAFEIFRATGLLLVAYSDLSIGLMFAMFGYIWFIMTPVQEILSIQYSLASAKAAI
                     GRINKVLELKTESDGNIELKDKKIDITLKNLTFSYTEDKTTLKNISFSIKSGEKVAII
                     GASGSGKTTISQLIAGFYAKNSGDILYNDISIDNISKKSLREHIFLVLQMPILFNNSL
                     RFNLTMGEDI"
BASE COUNT          427 a          178 c          132 g          470 t
ORIGIN      
        1 tatatcttca cccattgtaa ggttaaatcg taaagaatta ttaaataaaa taggcatttg
       61 aagaaccaaa aatatatgct ctcttaaact cttttttgaa atattatcaa tacttatatc
      121 gttgtataaa atatcaccac tattttttgc ataaaatcca gctataagtt gtgaaatagt
      181 agtttttcca cttccactag ctccaattat tgcaactttt tcaccacttt ttatagaaaa
      241 actaatattt tttaatgttg ttttatcttc tgtataagaa aaagtaagat ttttaagagt
      301 tatatctatc ttcttatctt ttaactctat atttccatca ctttcagttt ttagttctaa
      361 aactttgtta attcttccaa tagctgcttt tgcactagca agagagtatt gaatagataa
      421 aatctcttga acaggagtca taataaacca aatgtatcca aacatagcaa acataagtcc
      481 aattgataaa tcactataag ctactaataa aagtcctgtt gctctaaata tttcaaaagc
      541 aaaaaggaaa attgtatatg aaaacttttc ataggctaca cttttgtagt tgaactcatt
      601 tgaagctctt tttatatctt cagctttttt tatagatgta tcaaaaaaat tctcttcttt
      661 attacttgct tttatttgag aaaataggtc taaagtctca tttatattat tttggaaagc
      721 ttcaatagtg gcattttcct cttttttaag ttcacctgtt tttttagcaa ttcttcttga
      781 tattattgca attgttggtt gagttaaaag tatcattaat cctaaaacaa aatctatttt
      841 tataattaca aaaccaacag ctaaaagagt caaagaagaa gttataagtt tgcttgaaac
      901 atttacaata aaactatcaa gagtgtttac atctgttatt aagtttgcag atatctttcc
      961 acttcctaaa ctttcatatt cattcatatt tacttttttt agatgattta ggagttttac
     1021 tctaatgtca aatattacta tttttgatat ttttgtaaat atttttgtat ttataactcc
     1081 aaaaataaag tgaaaagctc gtaaacaaat cacaacagca gtaacaattg caacataata
     1141 aaaagcattt ccgctaccaa aaaactcatt tatattgttt acaaaaaagt ctggtttatt
     1201 tagtaat
//
LOCUS       DAOQAN010000489         1206 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_186718_length_1206_cov_2.374457, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000489 DAOQAN010000000
VERSION     DAOQAN010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_186718_length_1206_cov_2.374457"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..81)
                     /locus_tag="PKO10_07220"
     CDS             complement(<1..81)
                     /locus_tag="PKO10_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase"
                     /protein_id="HNW66973.1"
                     /translation="MSKITGKVWNFGANIDTDVIIAARYLN"
     gene            247..>1206
                     /locus_tag="PKO10_07225"
     CDS             247..>1206
                     /locus_tag="PKO10_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 factor domain-containing protein"
                     /protein_id="HNW66974.1"
                     /translation="MSTKDINKTIEQLIKEYKDSILTYEKIIKIFPKAPTGATIKKIL
                     ALVQLYNVKVISSQEQAKLLNDEEAKKRKEQREKLIEAEDDEFDLLKNKELLEWSRSD
                     SPVRMYLREMGQIPLLTKDEEIEISKRIEMGEDIILDAICYVPYLIDFILEYKEPLVN
                     RERKVKELFKNFDDDTEEEEEEEEEEIEDYEDDLSADVEDDEKKLSGAKQKKLDKRAQ
                     TIIEAFKNLEKQKKEWLKFQAKEVPKSDDEVDLMTFDLAVAFKKRLLKDALLDLGPTS
                     KLITEIVKAMETSLKSDVGFDSELKRLEYKLPLFNETLQKNHQK"
BASE COUNT          515 a          145 c          186 g          360 t
ORIGIN      
        1 atttaaatat cttgcagcta taataacatc cgtatcaata tttgcaccaa aattccaaac
       61 tttacctgtg attttactca tacctaattc cttattttta ttaaccatta aatattaagt
      121 aaaaatttta tcctaacttt tagtaaactt tgtttaaaga ctcttctaca tagttcaata
      181 taatcatatc tttaaataat ttcggtataa tgtcttccta tttaaaaagg aggatttcct
      241 aaaacaatga gtactaaaga tataaataaa actatcgagc agttaatcaa agagtataaa
      301 gactcaatat taacttatga gaaaattata aaaatttttc caaaagctcc aacaggtgca
      361 acaattaaaa aaattcttgc tcttgttcag ctatataatg taaaagttat aagttctcaa
      421 gaacaagcaa aacttctaaa tgatgaagag gctaaaaaaa gaaaagagca aagagaaaaa
      481 ctaattgaag cagaagatga tgagtttgat ttactaaaaa acaaagagct tttagagtgg
      541 tcaagatctg attctcctgt aagaatgtat ttaagagaga tgggacaaat accactttta
      601 acaaaagatg aagagattga aatatcaaaa agaatcgaga tgggagagga tattattctt
      661 gatgctattt gttatgttcc ttatctaata gactttattt tagaatataa agaacctctt
      721 gtaaatagag agcgaaaagt aaaagagcta ttcaaaaact tcgatgatga tactgaggaa
      781 gaagaggaag aagaagagga agaaattgaa gattatgaag atgatttatc tgctgatgta
      841 gaagatgatg agaaaaaatt atctggtgca aaacagaaaa aactagataa aagagctcaa
      901 acaattatag aagcttttaa aaatcttgaa aaacagaaaa aagagtggct aaaatttcaa
      961 gcaaaagaag tacctaaatc tgatgatgaa gtagatctta tgacttttga tttagcagtt
     1021 gcatttaaaa agagattatt aaaagatgct ttacttgatt taggacctac ttcaaaatta
     1081 ataactgaaa tagtaaaagc aatggaaaca tctttaaaat ctgatgttgg ttttgatagt
     1141 gaattaaaaa gattagagta caaactacca ctttttaatg aaacattaca aaaaaatcac
     1201 caaaag
//
LOCUS       DAOQAN010000490         1205 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_187021_length_1205_cov_1.909565, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000490 DAOQAN010000000
VERSION     DAOQAN010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1205
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_187021_length_1205_cov_1.909565"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="PKO10_07230"
     CDS             <1..397
                     /locus_tag="PKO10_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HNW66975.1"
                     /translation="EAGYDEALLRDDQGYIAEASGACFFIVKDGKLISPPNDNSLESI
                     TQATAIDLAKDMGIEVVRRRITREEIYVADEAFFTGTAAEITPIREVDARVIGAGCRG
                     EITEKIQSAYFDVVTGKNPKYTKYLTYIN"
     gene            441..>1205
                     /locus_tag="PKO10_07235"
     CDS             441..>1205
                     /locus_tag="PKO10_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HNW66976.1"
                     /translation="MPIDNDYFKNRQQGNKPSGGGGGNFQPPFETPEFFKNFGKKAGM
                     LYLVIILIGALFLFKPFVIIESGQVGIKATTGKYDKEPLNPGFHFYIPVIQRVIVVDT
                     KVRLLTYMNTQSIGSFDQSIKNNPAINVLDSRGLPISIELTVQYNIIASGVPETIATW
                     GPSWEDKIVNQVVGEVARSVLGGYNAEVLPMKRNEVAESLDRLIKEKVNERSKGAVIV
                     ESVQLKEIVLPEKIKEQIEKVQIANQEAERVRYEVQR"
BASE COUNT          463 a          146 c          237 g          359 t
ORIGIN      
        1 agaagctgga tatgatgaag cactattaag agatgaccaa ggttatattg cagaagctag
       61 tggtgcatgt ttctttattg ttaaagatgg aaaattaata tctccaccaa atgacaactc
      121 acttgaatct attactcaag caactgcaat tgatttagca aaagatatgg ggattgaggt
      181 tgttagaaga agaataacta gagaagagat ttatgtagct gatgaagctt tctttacagg
      241 aacagcagca gagattactc caattagaga agttgatgcg agagttattg gagctggatg
      301 tagaggagag attacagaaa agattcaatc tgcatatttt gatgtagtaa ctggaaaaaa
      361 tccaaaatat acgaagtatt taacatatat taactaattt taaatagtat attagattaa
      421 aaaaaaagat aaggaagatt atgccaatag ataacgacta ttttaagaac agacagcaag
      481 ggaataaacc aagtggtggt ggaggtggaa attttcaacc accatttgaa actccagaat
      541 ttttcaaaaa ttttggtaaa aaagcaggta tgctctacct tgtaattata ctaattggtg
      601 cactattttt gtttaaacca tttgttatta ttgaatcagg acaagttggt attaaagcaa
      661 caacaggaaa atatgataaa gagcctttaa atccaggatt ccatttttat attcctgtaa
      721 ttcaaagagt tattgttgtt gatacaaaag ttagactttt aacatatatg aacactcaaa
      781 gtattggttc ttttgatcaa agtatcaaaa ataaccctgc aataaatgtt ttagattcaa
      841 gaggtttacc aatctctatt gaattaacgg ttcaatacaa tattattgca agtggagttc
      901 ctgaaacaat tgcaacttgg ggaccatctt gggaagataa gattgtaaat caagttgttg
      961 gagaagttgc aagaagtgtt cttggtggat acaatgctga agttcttcct atgaaaagaa
     1021 atgaagttgc tgaaagtctt gatagattaa tcaaagaaaa agtaaatgaa agatctaaag
     1081 gtgcagttat tgttgaatct gttcagctaa aagagattgt tcttcctgaa aagattaaag
     1141 agcaaataga aaaagttcaa attgcaaatc aagaagcaga aagagtaaga tacgaagttc
     1201 aaaga
//
LOCUS       DAOQAN010000491         1202 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_187596_length_1202_cov_3.081081, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000491 DAOQAN010000000
VERSION     DAOQAN010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1202
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_187596_length_1202_cov_3.081081"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..775
                     /gene="prmC"
                     /locus_tag="PKO10_07240"
     CDS             <1..775
                     /gene="prmC"
                     /locus_tag="PKO10_07240"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HNW66977.1"
                     /translation="KEVEILIMHLLDKNVIWLHLNYDKAFTKELELEKLVKKRATNFP
                     LEYIIKKASFYGEQFLVDVDVLIPRPETELLVENAFEILKEVKNQEKSIKVLEIGTGS
                     GIISVMLATLLKDKNIDFIAVDINPKAIELARKNAKKFEVLERIDFRLSNLFENVKED
                     DIFMLISNPPYIANDYKLPKNVEFEPSNALFGGVVGDELLKNIIDGANSRKIPYVLCE
                     MGFDQKKSLEEYFKEFNVEFYSFYKDYEKFDRGFTLKFK"
     gene            802..>1202
                     /locus_tag="PKO10_07245"
     CDS             802..>1202
                     /locus_tag="PKO10_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013135536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family peptidase"
                     /protein_id="HNW66978.1"
                     /translation="MFKEFHIENFENSSKLLEELLNNSRVEVEKLLEIKNKTYKNFVL
                     PFAEIGEKINEFITPIFHLDSVKNSKITSKVYEECLPLISKYESEISQNENIYTSFKN
                     IQSNEKSTLNNIQFKVLENEIRDFELSGCQL"
BASE COUNT          504 a          103 c          196 g          399 t
ORIGIN      
        1 aaaagaggta gagatattaa tcatgcattt actggataaa aatgtgattt ggttacatct
       61 taattatgat aaagctttta caaaagagct agaattagaa aaattggtaa aaaaaagagc
      121 tacaaatttt cctttagagt atattataaa aaaagcctct ttttatggtg aacaattttt
      181 agtagatgtg gatgttttaa ttccaagacc agaaactgag cttttagttg aaaatgcttt
      241 tgagatttta aaagaggtaa aaaatcaaga aaaatcaatc aaggttcttg aaattggaac
      301 aggaagtgga ataatttctg ttatgctagc aacactttta aaagataaaa atatagattt
      361 tatagctgtt gatataaatc caaaagctat agaattagca agaaaaaatg caaaaaaatt
      421 tgaagtttta gaaagaatag attttagact tagtaattta tttgaaaatg taaaagagga
      481 tgatattttt atgcttattt caaatccacc gtacattgca aatgattata aattaccaaa
      541 aaatgtagag tttgagccat ctaatgcact ttttggtgga gttgtaggag atgagctttt
      601 gaaaaatatt atagatggtg caaacagtag aaagattcct tatgtgcttt gtgaaatggg
      661 atttgatcaa aaaaagagtt tagaagagta ttttaaagag tttaatgtag aattctactc
      721 tttttataaa gattatgaaa aatttgatag aggatttact ctaaaattta aataaaatga
      781 ataataataa aggataaaaa tatgtttaaa gagtttcata tagagaattt tgagaattca
      841 agtaagcttt tagaagagct tttaaataat agtagagttg aagttgaaaa acttttagaa
      901 attaaaaata aaacttataa aaattttgta ttaccttttg ctgaaatagg ggagaagata
      961 aatgaattta taactccaat attccattta gattctgtaa aaaattcaaa aataacttct
     1021 aaagtatatg aagagtgcct tcctcttatc tcaaaatatg agagtgagat aagtcaaaat
     1081 gagaatattt atacatcttt taaaaatata cagtctaatg aaaaaagtac tttaaataat
     1141 atacaattta aagtattaga aaacgagata agagattttg aattatcagg ttgccaatta
     1201 ga
//
LOCUS       DAOQAN010000492         1201 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_188002_length_1201_cov_1.795812, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000492 DAOQAN010000000
VERSION     DAOQAN010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1201
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_188002_length_1201_cov_1.795812"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..>1201)
                     /locus_tag="PKO10_07250"
     CDS             complement(4..>1201)
                     /locus_tag="PKO10_07250"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HNW66979.1"
                     /translation="SDEDIFLEIKKEREEIGYYSLPHSDITNLKKELDSLNFKQKNIA
                     IVGIGGSTLGTYAIYNFLKYHKQKNKSLKKEIFFFESTDPVNLNGTISQFDLEDTLFI
                     VISKSGTTIETISIFKYLSSIIKMDKSNLLVITENDSKLNSFAKVNDIKTFDIPKNVG
                     GRFSVLSNVGLVPLYLAGFDIDELLNGARKISTSFFEQYELFTHLIKKARTYYEYKDV
                     YNINAVFSYSQLLEGFNKWYIQLWGESLGKIDANNTNQGLTPIGLLGPVDQHSFLQLI
                     VEGKRDKTVTFIKIKDFKDDTKIAPISLSGLEELDYINNLDFKELINLQADATIASVK
                     EYKKDIPIDLIELEEISEYEIGKLLFYYELLTSIVGKFLRINTYDQPGVEGGKIILKE
                     MLKNKN"
BASE COUNT          403 a          178 c          132 g          488 t
ORIGIN      
        1 tttttaattt ttgtttttta acatctcttt taatataatt tttccacctt caactcctgg
       61 ttgatcataa gtgtttattc ttaggaattt tcctacaatt gaagttaaaa gctcatagta
      121 aaatagaagt tttcctattt catattcgct tatctcttct aactctatta aatcaatagg
      181 aatatctttt ttatactctt ttactgatgc tattgtagca tctgcttgaa gatttattaa
      241 ctctttaaaa tcaagattat ttatataatc aagctcttct aatccactta aactaatagg
      301 agcaattttt gtatcatctt taaaatcttt aatttttata aatgttacag ttttatctct
      361 tttaccttct acaattaact gtaaaaatga gtgttgatca acaggtccta aaagcccaat
      421 tggagttaaa ccttgatttg tgttatttgc atcaattttt ccaaggcttt ctccccaaag
      481 ttgaatatac cacttattaa aaccttcaag aagttgagag tatgagaaaa ctgcatttat
      541 gttgtaaaca tctttgtatt catagtaagt tctagctttt tttattaaat gagtaaaaag
      601 ttcatactgt tcaaaaaatg aagtagatat ttttctagca ccatttaaaa gctcatcaat
      661 atcaaaacca gctaaatata aaggcactaa tccaacattt gataaaacag aaaatcttcc
      721 accaacattt tttggaatat caaaagtttt aatatcattt acttttgcaa aactatttag
      781 tttgctatcg ttttcagtta ttactaaaag atttgattta tccattttta taatagaact
      841 taagtattta aaaatcgaaa tagtttcaat agttgtacca gattttgaaa taactataaa
      901 taaagtatct tctaaatcaa attgagagat tgttccattt aagtttacag ggtctgtact
      961 ttcaaagaaa aaaatctctt tttttaaaga tttatttttt tgtttgtggt attttaagaa
     1021 attgtatatt gcataagtac ctaaagtact tccaccaatt ccaactattg caatattttt
     1081 ttgtttaaaa ttaagagaat ctaactcttt ttttaagttt gttatatcac tatgaggcaa
     1141 agagtagtaa cctatctctt cacgctcttt ttttatctct aagaatatat cttcatcact
     1201 t
//
LOCUS       DAOQAN010000493         1196 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_189145_length_1196_cov_3.226994, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000493 DAOQAN010000000
VERSION     DAOQAN010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_189145_length_1196_cov_3.226994"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="PKO10_07255"
     CDS             <1..214
                     /locus_tag="PKO10_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011139024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HNW66980.1"
                     /translation="DTTKSILFVSGRLSSEMVTKAVMHKIPIVVSRTAPTYLGVQTAH
                     KHGLTLIGFARGKKMNLYTHSGRIDV"
     gene            207..632
                     /locus_tag="PKO10_07260"
     CDS             207..632
                     /locus_tag="PKO10_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ModE family transcriptional regulator"
                     /protein_id="HNW66981.1"
                     /translation="MSSIDDKLDFNLSDEQKRIVFENLDENKKLSCLKAFKVAKKIKV
                     EAKDMSAITKFLGIKITDCELGVFGSLDFLAKDEDIYNRLKNIYKEQKIPCENLWQEA
                     KNSSLKIVGSTVKNSDIEVSYCQLGCFREKKGLKSGNQS"
     gene            616..978
                     /locus_tag="PKO10_07265"
     CDS             616..978
                     /locus_tag="PKO10_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNW66982.1"
                     /translation="MEIKVKIWIEDNKQNLIFGSGKTEILKEIEKPGSISDASKNLNM
                     NYKKTWSHIKILEEFIEDSLVVTKKGRAVDSGSQLTTKAKELIELYEILDKDIKEYSK
                     KRFNELFLSENLIKIKDK"
     gene            981..>1196
                     /locus_tag="PKO10_07270"
     CDS             981..>1196
                     /locus_tag="PKO10_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HNW66983.1"
                     /translation="MYKLNFLQYKNMQNLHIKDSLSLNILSNNEDYSSLEDSFKKEFA
                     FESLNYFSFCKSGFLSLLLQLNKKGKIA"
BASE COUNT          498 a          111 c          209 g          378 t
ORIGIN      
        1 tgatactaca aaatcaattt tgtttgtaag tggaagatta agttcagaga tggttacaaa
       61 agctgtgatg cacaaaatcc caatagttgt atcaagaact gcaccaactt atttgggtgt
      121 tcaaacagca cataaacacg gtcttacact tattggattt gcaagaggta aaaagatgaa
      181 tctttatact catagtggaa gaattgatgt ctagtattga tgataaatta gattttaatt
      241 tgagtgatga gcaaaaaaga atagtttttg aaaatcttga tgagaataaa aaactatctt
      301 gtttaaaggc atttaaagta gctaaaaaga taaaagttga agctaaagat atgagtgcga
      361 ttacaaaatt tttggggatt aaaataacag attgtgagct tggagtattt ggctctttag
      421 atttcttagc taaagatgaa gatatttata atagattaaa aaacatttat aaagagcaaa
      481 aaattccttg tgaaaatttg tggcaagaag caaaaaactc atctttaaaa atagttggat
      541 caactgttaa aaactctgat attgaagtaa gttattgcca actgggatgt tttagagaga
      601 aaaaagggct taaaagtgga aatcaaagtt aaaatttgga ttgaagataa taagcaaaat
      661 cttatttttg gaagtggaaa aacagagatt ttaaaagaga ttgaaaaacc aggttcaatt
      721 agtgatgctt caaaaaattt aaatatgaac tataaaaaaa cttggagtca tataaaaatc
      781 cttgaagagt ttattgaaga tagtttagtt gttacaaaaa aaggaagagc tgttgatagt
      841 ggatctcaac taactactaa agcaaaagag ctgatagaac tttatgagat tttagacaaa
      901 gatataaaag agtattcaaa aaagagattt aatgaacttt ttttaagtga aaacttaata
      961 aaaataaagg ataaataaaa gtgtataaac taaacttttt gcagtataaa aatatgcaaa
     1021 acttacatat taaagatagt ttatctttga atattttatc aaataatgaa gattatagta
     1081 gtttagaaga tagttttaaa aaagagtttg catttgaaag tttaaactac ttctcttttt
     1141 gtaagagtgg ctttttatct ctattgcttc aactaaataa aaaaggaaaa attgca
//
LOCUS       DAOQAN010000494         1196 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_189284_length_1196_cov_1.822962, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000494 DAOQAN010000000
VERSION     DAOQAN010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_189284_length_1196_cov_1.822962"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..177)
                     /gene="rpmC"
                     /locus_tag="PKO10_07275"
     CDS             complement(<1..177)
                     /gene="rpmC"
                     /locus_tag="PKO10_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HNW66984.1"
                     /translation="MNYTDIKDKSLSELQALLKEKKVLLFELKAKLKTMQLTNTSELR
                     VAKKDIAKIQTAITA"
     gene            complement(164..589)
                     /gene="rplP"
                     /locus_tag="PKO10_07280"
     CDS             complement(164..589)
                     /gene="rplP"
                     /locus_tag="PKO10_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006565271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HNW66985.1"
                     /translation="MLMPKRTKFRKMMKGRNRGEAHRGNSLAYGDFGIKAVEHGRVDS
                     RQIEASRVAMTRKVKRQAKVWIMVFPDKPLTAKPLETRMGKGKGSVDKWVMNIKPGRV
                     CFEMAGVDESLAREALALAMHKLPFKTKFITRDSENELY"
     gene            complement(592..>1196)
                     /gene="rpsC"
                     /locus_tag="PKO10_07285"
     CDS             complement(592..>1196)
                     /gene="rpsC"
                     /locus_tag="PKO10_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009649492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HNW66986.1"
                     /translation="EDDKIRKFVKKELYYAGIAQTVIERTAKKVRVTVVAARPGIIIG
                     KKGADVEKLKDSLSKLVGKEIAVNIKEERKPQTSALLSAENVAQQLERRVAFRRAMKR
                     VMQNALKGGAKGIKVSVSGRLGGAEMARTEWYLEGRVPLHTLRARIDYGFAEAHTAYG
                     CIGIKVWIFKGEVLAKGIPTEKSETPESKPKRRPAKKRGK"
BASE COUNT          317 a          253 c          144 g          482 t
ORIGIN      
        1 agctgtaata gcagtctgaa ttttagcaat atcttttttt gccactctta attcagatgt
       61 atttgttaac tgcatagttt ttagcttagc ttttaattca aaaagaagca cctttttttc
      121 ttttaataac gcttgtaatt cgctcaagct tttatcttta atatcagtat agttcatttt
      181 cgctatctct tgttataaat ttagttttaa atggtagttt atgcatagct aaagctaaag
      241 cttctcttgc taaactttca tctactccag ccatttcgaa acaaactctt cctggcttaa
      301 tgttcattac ccatttatca acactacctt tacctttacc cattcttgtt tcaagtggtt
      361 tagcagttag tggtttatca gggaatacca taatccaaac tttcgcttgt ctttttactt
      421 ttcttgtcat ggcaacccta gaagcttcaa tttgtcttga atctactcgt ccatgctcaa
      481 cagctttaat tccgaaatct ccataagcta aagagttacc tctgtgagct tcacctcgat
      541 ttcttccttt catcattttt ctaaattttg ttcttttagg cattaacata attatttacc
      601 tcttttcttt gctggtcttc tttttggttt agactcagga gtttcagatt tttcagttgg
      661 aattcctttt gcaagaactt cacctttaaa tatccaaact ttaatcccaa tacaaccata
      721 agctgtatga gcttctgcaa aaccataatc aattctagct cttaatgtat gtaaaggaac
      781 tcttccttct aaataccact cagttcttgc catttcagct ccaccaagtc ttccagaaac
      841 agatacttta ataccttttg ctccaccttt aagtgcattt tgcataactc ttttcatagc
      901 tcttctaaat gcaactcttc tttctaattg ttgagcaaca ttctcagctg aaagtagtgc
      961 agaagtttga ggttttctct cttctttaat atttacagct atctcttttc ctacaagttt
     1021 agataaagag tcttttagtt tttcaacatc tgcacctttt ttaccaatga taattccagg
     1081 tctagcagca acaactgtaa ctcttacttt ttttgcagtt ctttcaataa cagtttgagc
     1141 aattccagca taatataact cttttttaac aaattttctt attttgtcat cttcag
//
LOCUS       DAOQAN010000495         1194 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_189769_length_1194_cov_2.060579, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000495 DAOQAN010000000
VERSION     DAOQAN010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_189769_length_1194_cov_2.060579"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="PKO10_07290"
     CDS             <1..250
                     /locus_tag="PKO10_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016646093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNW66987.1"
                     /translation="RLELKFLDSGGGIDEALISRVFEPYFTTKHKSQGTGLGLPIVDK
                     IVRERHNASIEIYNEDFIYNEKKYRGFSFKIIFERLNH"
     gene            complement(294..>1194)
                     /locus_tag="PKO10_07295"
     CDS             complement(294..>1194)
                     /locus_tag="PKO10_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNW66988.1"
                     /translation="IKKAMNAEYAGFFDIGMVDRNEENAIVVHGTMVMVRLSAMMEVG
                     GWGTDTIVEDSELGLRLFEAGYIAHYTNRRYGFGLLPDTVEAFKTQRHRWAYGAIQIL
                     KKHWREFKPSSKRLSPPQKNKFVTGWFFWLSDAMGPIMAVMNIIWVPVIIFVGVTIPT
                     IPLTIPIITAFIVNILHTFILYRTKVKATFKEILLSSIASMSLQLIIFKAVFDGFIKD
                     GLPFKRTEKGGKAKKSANPIKYETILCVLLLTAFVSLIYTNKSGIIEIYVFAATIFIQ
                     TIPYISAIIMRILELYSLKNQKA"
BASE COUNT          427 a          184 c          160 g          423 t
ORIGIN      
        1 tagacttgaa ttaaagtttt tagatagtgg tggaggaatt gatgaagcac ttatatctag
       61 agtttttgag ccatatttta caacaaaaca taaatctcaa ggaacaggac ttgggcttcc
      121 gatagttgat aaaattgtaa gagaaagaca taatgctagt attgaaattt ataatgaaga
      181 ttttatttat aatgaaaaaa agtatagagg ttttagcttt aaaattattt ttgaaagatt
      241 aaatcattaa ttttttgtaa atatacttat attttatgaa aatcaaaaag ctcttaagct
      301 ttttgatttt ttaaagagta taactctagt attctcataa taattgcaga gatataaggg
      361 atagtttgaa taaatattgt tgctgcaaat acataaatct ctataattcc agatttattt
      421 gtgtatatta aagatacaaa ggctgttaaa agtaaaacac ataagatagt ttcgtatttt
      481 atagggtttg cactcttttt tgcttttcca cctttttctg ttcttttaaa tggtaatcca
      541 tcttttataa atccatcaaa tacagcttta aatattatta attgcaaact catagaggca
      601 atagagctta gtaaaatttc tttaaaagtt gcttttactt ttgttctata aagtataaat
      661 gtatgcaaaa tatttactat aaatgcagta ataataggaa tagtaagagg aattgtagga
      721 attgtaacac ctacaaagat aattacagga acccaaataa tattcataac agccataatt
      781 ggtcccatag catcacttaa ccagaaaaac caacctgtaa caaatttatt tttttgtggc
      841 ggacttaatc tttttgaact aggtttaaac tctctccagt gctttttaag aatttgaatt
      901 gcaccataag cccatctatg tctttgagtt ttaaatgctt caacagtatc tggtaaaagc
      961 ccaaacccat atcttctatt tgtatagtgc gcaatataac cagcttcaaa tagtctaagc
     1021 cctaattcac tatcttcaac aatagtatca gttccccaac caccaacttc catcatagca
     1081 ctaagtctta ccataaccat tgttccatga acaacgattg cattttcttc atttctatca
     1141 accataccaa tatcaaagaa acctgcatac tcagcattca tagctttttt gata
//
LOCUS       DAOQAN010000496         1188 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_191154_length_1188_cov_4.113857, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000496 DAOQAN010000000
VERSION     DAOQAN010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_191154_length_1188_cov_4.113857"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(104..724)
                     /locus_tag="PKO10_07300"
     CDS             complement(104..724)
                     /locus_tag="PKO10_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66989.1"
                     /translation="MTKKDLLLIHSFIDSKNPKEVLNFAHLKDDGIYATDTKKCIMFS
                     IPMLDLDLFLEKKILKGFIAATHKDSIISFDGLGYMKNGTAKLNCDTFDYSDAVGVDF
                     DYILNQKFSNQFFLESIDDLQFELAQRNCFIDEEHINPIIAYAECSGFNIFYNEQKEE
                     LSNGIKVKNSGVAKIIGRYSTEDESNIVKFTAVIMGRTFESKAIEE"
     gene            complement(735..995)
                     /locus_tag="PKO10_07305"
     CDS             complement(735..995)
                     /locus_tag="PKO10_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66990.1"
                     /translation="MKKLIMLVLLASSLFAVDCSYWLKKTYEDITLLHLVVADEEYSE
                     VNSIYRRFLESSRNTIAICDSEEITNTMFILRKDALELLNKK"
BASE COUNT          448 a          201 c          101 g          438 t
ORIGIN      
        1 gtattctatg cttttcactc atattagctt gactaaaatc atattgctct acaaaggcaa
       61 tattatcacc aaatatattc tcttttttat ttattagttc catttattcc tctatcgctt
      121 ttgattcaaa agttcttccc ataataacag cagtaaactt tactatattt gactcatctt
      181 ctgtagaata tctaccaatg atttttgcaa caccactatt ttttacttta attccatttg
      241 ataactcttc tttttgctca ttataaaaaa tattaaatcc actacattca gcataagcta
      301 taataggatt tatatgttct tcatctatga agcaatttct ttgagccaac tcaaactgta
      361 aatcatctat tgattcaaga aaaaattggt tagaaaattt ttgatttaaa atataatcaa
      421 aatcaacacc aacagcatca ctataatcaa aagtatcgca atttaattta gcagtaccat
      481 ttttcatata tcctaaacca tcaaaagata tgatactatc tttatgtgta gcagcaataa
      541 aaccttttaa tatctttttt tctaaaaata aatccaaatc caacatagga atagaaaaca
      601 taatacactt ttttgtatca gtagcataaa taccatcatc ttttaagtga gcaaaattca
      661 aaacctcttt aggatttttg ctatcaataa aagaatggat caataataaa tcttttttag
      721 tcataataaa ttccttattt tttatttaga agctctaaag catcttttct aagtataaac
      781 atagtatttg taatctcttc actatcgcaa atagctatag tatttcttga actttcaaga
      841 aatctacgat aaatactatt aacctctgaa tactcttcat cagcaacaac aagatgtaga
      901 agagttatat cttcataagt tttctttagc caatagctac aatcaacagc aaataaacta
      961 ctggctaaca aaaccaacat aatcaacttt ttcatatcaa tcctttataa taagataaag
     1021 aaataatact ataacaaaat aaattaaagc ttaaaattat gcttttaaaa taaatttttc
     1081 acaaattaat tcataaagtt ttttagtagc ttcaatatct gataaagcat tatgagcttc
     1141 tatttcaata tcaaaataat tacacatagt ttcaagctta tcattttc
//
LOCUS       DAOQAN010000497         1187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_191603_length_1187_cov_1.749117, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000497 DAOQAN010000000
VERSION     DAOQAN010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_191603_length_1187_cov_1.749117"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..923
                     /locus_tag="PKO10_07310"
     CDS             <1..923
                     /locus_tag="PKO10_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66991.1"
                     /translation="RFISVLIFMSLPGVLSASLLVNTAIIVTFFTLLYIYFYQKHKKH
                     LYYLLPFLLLVDNSFAILFLALFLFSLKTKDRKLLYISSILFVVSLLIYGISTDGKPR
                     GFLIDTVGIYAAIFSPILFLYFLYVIYRLIVIKQTDLTTYISATALILSILVSFRQKI
                     YIEDFAPYVVIAIPSMVKMFLHSYRVRLKEFRTNYNIAAILIVVMLGINVVFTFVNKP
                     LYLIIQNPQKHFVYQYHIAKELSNELKNRAINNIILDDKDLLLRLKFYEIEDGNDYYL
                     STKEFYNYDDKISIYYYEKELFTIYIKKLI"
     gene            920..>1187
                     /locus_tag="PKO10_07315"
     CDS             920..>1187
                     /locus_tag="PKO10_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNW66992.1"
                     /translation="MKKTFLILELVFVIVILGILYTVFTPKLPNYKLDEVTNRVLIYL
                     NYVRYKALIDDKFETEVSEWFKGRWTMKFMRCREDKGGGIYFTIY"
BASE COUNT          434 a          124 c          156 g          473 t
ORIGIN      
        1 atagatttat atctgttttg atttttatgt ctcttcctgg agttttaagt gcttcattgc
       61 ttgtaaatac agctattatt gtcactttct ttactctttt gtatatctat ttttatcaaa
      121 aacataaaaa acatttatac tatttattac catttttact acttgttgat aactcttttg
      181 caatactatt tttagcacta tttctttttt cattaaaaac aaaagataga aagttgttgt
      241 atatctcttc aattttattt gtagtttcac tgcttattta tggaatatca acagatggaa
      301 agccaagagg ttttttaata gatacagttg gaatttatgc agctatattc tctccaatac
      361 tatttttata ttttttatat gttatttata gattaatagt aataaaacag acggatttaa
      421 caacttatat ctctgcaact gcacttattt tatccatatt agtatctttt agacaaaaaa
      481 tatatattga agattttgcc ccttatgttg ttattgctat accatctatg gtaaaaatgt
      541 ttttacactc atatagagta agattaaaag agtttagaac aaattacaat atagcagcaa
      601 ttttaattgt ggtaatgttg ggaataaatg ttgtttttac atttgtaaac aaacctcttt
      661 atttaataat tcaaaatcct caaaaacact ttgtttatca atatcatatt gctaaagagt
      721 tatcaaatga gcttaaaaat agagctataa ataatataat attagatgat aaagatttgt
      781 tgttgagatt aaaattttat gaaatagaag atggaaatga ttactattta tcaacaaaag
      841 agttttataa ctatgatgat aaaatatcta tatactatta tgaaaaagag ttatttacaa
      901 tttacataaa aaaattaata tgaaaaaaac atttttaatc ctagaattgg tttttgtaat
      961 tgtaatttta ggtattttat atacagtttt tacaccaaag ttaccaaatt ataagcttga
     1021 tgaggtcaca aatagagttt taatatattt aaattatgta agatacaaag ctttaataga
     1081 tgataagttt gaaactgaag taagcgaatg gtttaaggga agatggacaa tgaagtttat
     1141 gagatgtaga gaagataaag gtggtggtat ctattttact atttata
//
LOCUS       DAOQAN010000498         1186 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_191694_length_1186_cov_3.727675, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000498 DAOQAN010000000
VERSION     DAOQAN010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_191694_length_1186_cov_3.727675"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="PKO10_07320"
     CDS             complement(<1..520)
                     /locus_tag="PKO10_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNW66993.1"
                     /translation="MIFLTFIITIFHDIISIGFKKAFKKSKISTQRRNFFKKTFDIGA
                     TSLILATNLKAIDNARHIELEVVDVEIKNLKKPYKIIQLSDIHIGGLIDKKFIEDLVK
                     KVNILNGDLVVITGDLVDTKLEFAKPALDELKNIKSKYGTYFIVGNHEYFHGVNPIID
                     YLNNLGIKVLENE"
     gene            complement(791..1069)
                     /locus_tag="PKO10_07325"
     CDS             complement(791..1069)
                     /locus_tag="PKO10_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNW66994.1"
                     /translation="MNEYLDYQSKEFLDLISMNVVKIRKSKGFSQLRLAVEMGYSSAS
                     YIGRMEIRKNGEHFNLIQLFKIAKILDVSIKDIFEGMDEIIKTKDTNY"
BASE COUNT          435 a          158 c          127 g          466 t
ORIGIN      
        1 attcattttc taaaacttta attccaagat tatttagata atcaattata ggattaaccc
       61 cgtgaaaata ctcgtgattt ccaactataa agtatgttcc atattttgat tttatatttt
      121 ttaattcatc taaggccggt tttgcaaatt ctaatttagt atccacaagg tcaccagtta
      181 taacaacaag gtcaccattt aaaatattta ctttttttac taaatcttct ataaattttt
      241 tatctattaa tccaccaata tgaatatcac ttagttgtat tattttatat ggttttttta
      301 aatttttaat ctctacatct acaacttcaa gttcaatatg tcttgcattg tcaatcgctt
      361 ttagatttgt tgcaagaatt agtgaagttg caccaatatc aaaagttttt ttgaaaaaat
      421 ttcttctttg tgttgatatt tttgattttt tgaaagcttt tttaaatcca attgaaatga
      481 tatcgtgaaa aatagtgata ataaatgtta aaaaaatcac cccaattggt agggaaagga
      541 gaaaatagag ccagttaggc actagtggaa agtatctagc cattgggtac aataaagcac
      601 caaaaaatgt taaatataat gcaaagccaa gatacttcct agttttaaga ctaaaattta
      661 gtttatttat aaatcttttt tttatagtat aggtctgaag aaaaaatgca acaaaataaa
      721 ctaaaaagaa aatcataaaa ctcataaagg aattataaat atttagagtt aatagttttt
      781 aaatagataa ttaatagttt gtatcttttg tttttattat ctcatccatt ccttcaaaaa
      841 tatcttttat agatacatct aaaatttttg ctattttgaa aagttgaatt aaattaaaat
      901 gttcaccgtt ttttctaatc tccattcttc caatatatga agcactagaa tatcccatct
      961 caactgctaa tcttaattgg ctaaatcctt ttgattttct tattttaaca acattcatag
     1021 aaattaaatc taaaaactct tttgattgat aatctaaata ttcattcaat tttaacacct
     1081 tatatagtgt taatcttatg aagtttagca aatttaatgc aaccatatat atagtgttgc
     1141 atttatacac aacaattatt ttttatatta aaatctatgt aaaaaa
//
LOCUS       DAOQAN010000499         1186 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_191802_length_1186_cov_2.249337, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000499 DAOQAN010000000
VERSION     DAOQAN010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_191802_length_1186_cov_2.249337"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            14..124
                     /locus_tag="PKO10_07330"
     CDS             14..124
                     /locus_tag="PKO10_07330"
                     /inference="COORDINATES: protein motif:HMM:NF019819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HNW66995.1"
                     /translation="MLSRDKTISDEVLNKLLEKISNMGFDKSKLIYTIQE"
     gene            128..424
                     /locus_tag="PKO10_07335"
     CDS             128..424
                     /locus_tag="PKO10_07335"
                     /inference="COORDINATES: protein motif:HMM:TIGR03643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03643 family protein"
                     /protein_id="HNW66996.1"
                     /translation="MYFQKDSSREFNANKKKGAKPSNLSFTESDKNRLIEMAWQDRVS
                     FDTIKELYGFTENEVKNMMRDLLKRSSFKMWRKRVQGRATKHKLKVSYKTTRFQ"
     gene            448..>1186
                     /locus_tag="PKO10_07340"
     CDS             448..>1186
                     /locus_tag="PKO10_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HNW66997.1"
                     /translation="MKTIAISGASGFVGQSLVDFFSKQNYKVVQIKRDILNNSLKLEE
                     LINSSDVVINLSGANIINRWSESYKKLLISSRLDTTKKLVESLNRVEKKDKLFISTSA
                     VGIYDNKVKYDENGSFSNDFLSTLCQNWEKEAQKAKSESTKVAIFRFGIVLGKNGGAF
                     QKMITPFKLGLGGIIGSGKQHFSYIHIEDLIEVYKFVIENSLDGVFNCTAPTPTTNYE
                     FTKTLGRVLNRPTIFPIPEFVLKLIFSE"
BASE COUNT          491 a          116 c          211 g          368 t
ORIGIN      
        1 agagtattta tggatacttt cacgagataa aactattagt gatgaggtat taaataaact
       61 attagaaaaa atttcaaata tgggatttga taagtcaaaa cttatttata caatacagga
      121 gtaagatatg tattttcaaa aagatagctc aagagagttt aatgcaaata aaaagaaggg
      181 tgcaaaacca agtaatttat cttttactga aagtgataaa aatagactta ttgaaatggc
      241 atggcaagat agagtctctt ttgatactat aaaagagctt tatggtttta ctgaaaatga
      301 agttaaaaat atgatgagag atcttttaaa aagaagtagt tttaagatgt ggagaaaaag
      361 agttcaagga agagctacaa aacataaatt aaaagtaagc tataaaacga caagatttca
      421 gtaaaaataa ttatacaagg ataaaatatg aaaactattg caatatcagg tgctagtggg
      481 tttgttgggc aaagtttggt ggattttttt tcaaaacaaa attataaggt agttcaaatc
      541 aaaagagata ttttaaataa ctctttaaaa ctagaggaac ttataaattc aagtgatgtt
      601 gtaataaatc taagtggtgc aaatataata aatagatgga gtgaaagtta taaaaaactt
      661 ttgatttcaa gtagacttga tactacaaaa aaacttgttg aatctctaaa tagagttgaa
      721 aagaaagata aactatttat atcaacatca gctgttggaa tttatgataa caaagttaaa
      781 tatgatgaaa atggctcttt ttcaaatgat ttcttatcaa ctctttgcca aaattgggaa
      841 aaagaggcac aaaaagcaaa aagtgagagt acaaaagttg ctatttttag attcggaata
      901 gttttaggaa aaaatggtgg agcatttcaa aagatgataa ctccttttaa attaggtctt
      961 ggtggaatta taggaagtgg taaacaacac ttttcatata ttcacataga agatttgata
     1021 gaagtttata agtttgtaat agaaaatagt ttagatggag tatttaattg tacagcgcca
     1081 actcctacaa caaactatga gtttacaaag acactaggaa gagttttaaa tagaccaaca
     1141 atttttccta tcccagagtt tgttttaaaa ctgatattta gtgagg
//
LOCUS       DAOQAN010000500         1183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_192438_length_1183_cov_4.267730, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000500 DAOQAN010000000
VERSION     DAOQAN010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_192438_length_1183_cov_4.267730"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(147..923)
                     /locus_tag="PKO10_07345"
     CDS             complement(147..923)
                     /locus_tag="PKO10_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HNW66998.1"
                     /translation="MKKIRNLTLSIVLASTLFSGCATTELQTNVRMSQSVFIDPVKKE
                     LRTVFISSKNTSGQPINLEQSLSNELQAKGYKIVDDPDEATYILMVNVLYCDKKQENN
                     VAGAALGAGAVGAGVSAYNNGGAGQAIGVGLGAAVIGGLIGKATEDTIYQMQVDILIK
                     ERTKGSVLTQNSTVAGQASVRDGKKSGFVNSFGGDVRNTNATGQINSNIANDKTQYYE
                     EERKEYKTVMLAEATKMNLTLQEATPILEKQISNQISGLF"
     gene            complement(981..>1183)
                     /locus_tag="PKO10_07350"
     CDS             complement(981..>1183)
                     /locus_tag="PKO10_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014472988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW66999.1"
                     /translation="SCHVIVQNSKKDLPQNIIEKAASLCAKFSVDFGGTYEVDFTQRR
                     NVKIQHGANVLYNPYSTIVVKV"
BASE COUNT          357 a          209 c          134 g          483 t
ORIGIN      
        1 tgttgataaa tttccaagta ttttaaacat aaagcttacc ttaaactaaa attacaatat
       61 tttactaaaa tttcgctttt aaatcgattg tactattata ttttgagagt ttataaatgc
      121 actaaaattt agtgcatttt agttttttag aataatcctg agatttgatt tgaaatctgt
      181 ttttctaaaa ttggtgttgc ttcttgtaaa gttaaattca tttttgtagc ttctgctaac
      241 attactgttt tatactcttt tcgctcttct tcgtaatatt gagttttatc attagcaata
      301 tttgaattaa tctgtccagt agcatttgta tttcgaacat ctccaccaaa actatttaca
      361 aatcctgatt ttttaccatc tcttacacta gcttgtccag ctactgttga attttgtgtt
      421 aaaacagaac cttttgttct ctcttttatt aatatatcaa cttgcatttg ataaattgta
      481 tcttctgttg ctttaccaat aagtccgcca attacagcag ctcccaatcc aactccaata
      541 gcttgaccag ctcctccatt attatatgca gaaacacctg ctccaacagc tcctgctccc
      601 aaagcagctc ctgctacatt attttcttgt tttttatcac aataaagtac atttaccatc
      661 aaaatatatg tagcttcatc tgggtcatca acaattttat agccttttgc ttgaagttca
      721 tttgataaac tttgctcaag atttattggc tgtccgctag tatttttact agatataaat
      781 actgttctta actctttttt aactgggtca ataaatacac tttgagacat tcttacatta
      841 gtttgtagct ctgttgttgc acatccacta aatagtgttg aagctaaaac aatacttagt
      901 gttaaatttc taattttttt cattatattc tcctaaaaaa tattcaatgg attattatat
      961 ttaaatttta ttaactatta ttaaacttta acaacaatag tactgtatgg attataaagc
     1021 acatttgctc catgttgaat tttgacattt cttctttgtg taaaatcaac ttcatatgta
     1081 ccaccaaaat ctacactaaa ctttgcacat aaacttgctg ctttttctat aatattttgt
     1141 ggtaaatctt ttttactatt ttgtacaatc acatggcaag atg
//
LOCUS       DAOQAN010000501         1182 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_192799_length_1182_cov_2.474712, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000501 DAOQAN010000000
VERSION     DAOQAN010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1182
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_192799_length_1182_cov_2.474712"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="PKO10_07355"
     CDS             <1..275
                     /locus_tag="PKO10_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014469417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Bcr/CflA family drug resistance efflux
                     transporter"
                     /protein_id="HNW67000.1"
                     /translation="KDMNLILTVIIIASYMSMMAFIFGNCMSLAIEHFSKNAGVASGV
                     IGVLQFGLGALISSVALNFEDNGFFVIAFSITFLSIISFLIIRSYK"
     gene            complement(298..>1182)
                     /locus_tag="PKO10_07360"
     CDS             complement(298..>1182)
                     /locus_tag="PKO10_07360"
                     /inference="COORDINATES: protein motif:HMM:NF013259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase"
                     /protein_id="HNW67001.1"
                     /translation="ELNLEQLESILDILETFSLLKISFKDGQKIEFDSLNLRDIPEIF
                     NILNKVGLSSFFESSHSIKKVLTCPVNGLDTTQIFDVEDLAKKLNESFVENKNFFNLP
                     NKLQFVISGYREGCDAGFTPDVSFNAFKNNKDKIVFDLKILDKVVAQIGSSQIIKTAR
                     VIAAVYRDFGNREKNSNFKEFIKEFTLESFCDILSSNLDIKIDNNQDIKILKEPKKPR
                     MGINKSSKDGYSFIGLKSIKKEFTKDELKNIIENMKKYSTTKLKITHKSNIIILDVPT
                     KNSDNLVNSLKNSGLVLE"
BASE COUNT          374 a          167 c          126 g          515 t
ORIGIN      
        1 acaaagatat gaacttgatt ttgacagtta ttataatagc ttcatatatg agtatgatgg
       61 cattcatttt tggaaattgt atgtctttag caattgagca tttttcaaaa aatgctggag
      121 ttgctagtgg ggttattggt gttttacagt ttggattggg tgctttaata tcttctgttg
      181 ctctaaattt tgaagacaat ggattttttg tgatagcttt tagcatcact tttctatcaa
      241 ttatcagctt tttgattatt agaagttata aatagtttat atcttcaact atttatatta
      301 ttctaaaact agtccactat ttttaagaga gtttactaga ttatcactat ttttagttgg
      361 tacatctaag attataatat ttgatttatg agtaattttt aactttgttg tactatattt
      421 tttcatattt tctattatat tttttaactc atctttagtg aactcttttt ttatgctttt
      481 tagtcctata aaagagtaac catcttttga acttttattt attcccattc taggtttttt
      541 tggttctttt aatattttta tatcttgatt attatctatt ttaatatcca aatttgaact
      601 taaaatatca caaaaactct caagtgtaaa ctcttttata aactctttaa aattactatt
      661 tttttctcta tttccaaaat ctctatatac agcagctatt actcttgctg tttttattat
      721 ctgagaacta cctatttgag ctacaacctt atccaaaatt tttaaatcaa aaactatttt
      781 atctttgtta tttttaaaag cattaaaact aacatctggt gtaaatccag catcacaacc
      841 ttcacgataa ccacttatca caaattgcaa tttatttgga agattaaaga aatttttatt
      901 ttcaacaaaa ctctcattta atttttttgc taaatcttct acatcaaaaa tttgtgtagt
      961 atctagtcca tttacagggc atgtcaaaac tttttttata ctatgacttg actcaaaaaa
     1021 acttgatagc cctactttat ttaatatatt aaaaatctct ggaatatctc ttaaatttaa
     1081 actatcaaac tctatttttt gaccatcttt aaaagatatt tttaatagtg aaaaagtctc
     1141 taaaatatct aatattgact ctagctgttc taaatttagc tc
//
LOCUS       DAOQAN010000502         1182 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_192919_length_1182_cov_1.592724, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000502 DAOQAN010000000
VERSION     DAOQAN010000502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1182
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_192919_length_1182_cov_1.592724"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>941)
                     /locus_tag="PKO10_07365"
     CDS             complement(<1..>941)
                     /locus_tag="PKO10_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNW67002.1"
                     /translation="NLGLCTQILRAVEEEGYTKATPIQTKAIPVILSKKDVLAAAQTG
                     TGKTAGFTLPLLELLNKNYLKDRKSAVKALILTPTRELATQVGESVEVYGKYLPFKSA
                     VIFGGVGINPQKAILKKGVDIITATPGRLLDLVSQDCLDLSKVEFLVLDEADRMLDMG
                     FIHDIKKILSILPKHRQNLLFSATFSPEIKKLADSLLKTPVLIEVSKANSTSHKVEQT
                     VHHVDKERKKELLIHLLNKNSWKQVLVFTRTKHGANKLSEALIKDKITSAAIHGNKSQ
                     GARTKALDDFKAGTVRVLVATDIAARGIDIDQLPHVI"
     assembly_gap    942..951
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          384 a          188 c          156 g          444 t
ORIGIN      
        1 tattacatga ggaagttggt ctatatcaat tccccttgca gcaatatcag ttgcaactaa
       61 aactcttaca gttccagctt taaaatcatc caaagctttt gttcttgcac cttgagattt
      121 atttccatga attgcagctg aagttatttt atcttttatt aaagcttcac ttagtttatt
      181 tgcaccatgt tttgttcttg taaaaactaa aacttgtttc caactatttt tatttaaaag
      241 atgaattaaa agctcttttt ttctctcttt atcaacatga tgaacagttt gttcaacttt
      301 atgtgaagta ctatttgctt ttgaaacctc tattaaaaca ggagttttta gcaaactatc
      361 tgctaatttt ttaatctctg gtgaaaaagt agctgaaaaa agtagatttt gtctatgttt
      421 tggcaaaatt gataatatct ttttaatatc atgaataaat cccatatcaa gcattctatc
      481 agcttcatct aaaaccaaaa actctacttt tgataaatct aaacaatctt gagaaaccaa
      541 atcaagcaat cttccaggag ttgctgtaat aatatcaaca ccttttttca aaattgcttt
      601 ttgaggattt attccaactc ctccaaaaat aacagctgat ttaaatggaa gatattttcc
      661 ataaacttca acactttctc ctacttgtgt tgctaactct cttgttggag ttaaaataag
      721 tgcttttaca gcactttttc tatcttttaa atagttttta tttaataatt ccaaaagtgg
      781 aagtgtaaaa ccagctgttt ttccagttcc tgtttgagca gctgctaaaa catctttttt
      841 tgacaatata actggaatag cctttgtttg aataggagtt gcttttgtat agccctcttc
      901 ttctactgca cgaagaattt gcgtgcataa tcctaaattt annnnnnnnn nataagtacc
      961 ttttgtttta tgaacctaca aaagttatta gcttaagtta cgatctaggt tgaataattt
     1021 gagtttaaat gagttgtaga aaatctacat aaacgaagtc aggcatagac cgaagtatgc
     1081 cgaaattgtg agattatatc acaatttctt gattttgatt cataaattga aaagaatatg
     1141 tttttataaa aattaatttg ctacaatcaa caaaaatata ta
//
LOCUS       DAOQAN010000503         1179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_193630_length_1179_cov_2.072064, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000503 DAOQAN010000000
VERSION     DAOQAN010000503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_193630_length_1179_cov_2.072064"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1179)
                     /locus_tag="PKO10_07370"
     CDS             complement(<1..>1179)
                     /locus_tag="PKO10_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014469021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNW67003.1"
                     /translation="RDLNLEQNLIENLESLGYKTLTPIQENSLKYSLEKKDIIARAKT
                     GSGKTLAFCLPIINNLDKKKYRVQSLILAPTRELATQIAQTIREVLRSTPNIKVLTLC
                     GGVPYKPQVVSLSHEAHIVVGTAGRVLKHINEGNLKLENINTFVLDEADKMLDMGFFN
                     DISKVSSLLPKQRQTLLFSATFSNEIKNLALEFLNNPVNIEVEDEEQNIIKQVFYEVN
                     SRDLKDNLIEKLIKKYQANSTIIFCNQRVTCEELADSLFERDIDAITLHSDLDQKERD
                     ETLTIFSNKSYPVLIASDVASRGIDINNIDLVINYDLALNYKIHTHRIGRTARAGKGG
                     VAITFVLPNEAKSALEIKEHFPDIVFEDEKEIVFDNSFEIDSIFRSIFINGGKKQKLR
                     "
BASE COUNT          376 a          195 c          127 g          481 t
ORIGIN      
        1 ttctcaattt ttgttttttc ccaccattta taaagattga tctaaaaata gaatctatct
       61 caaaactatt gtcaaaaaca atctcttttt catcttcaaa tacaatatca ggaaaatgct
      121 cttttatctc taaggcactt ttggcttcat ttggtaaaac aaaagtaata gcaactccac
      181 ctttcccagc ccttgcagtt cttccaatac gatgagtatg tattttgtag tttaaagcta
      241 aatcatagtt tattactaaa tctatattat ttatatcaat tcccctactt gcaacatcac
      301 ttgctattaa aactggataa cttttatttg aaaatatagt aagagtttca tccctctctt
      361 tttggtctaa atcactatga agtgtaattg catctatatc tctttcaaaa agtgaatctg
      421 ctaactcttc acaagttact ctttgattac aaaaaattat tgtgctattt gcttgatact
      481 tttttatcaa tttttctatt aaattatctt ttaaatctct tgaatttact tcataaaaaa
      541 cttgttttat aatattttgt tcttcatctt caacttcaat attcactggg ttatttaaaa
      601 actctaaagc taaattttta atctcatttg aaaatgttgc agaaaaaaga agagtttgtc
      661 tttgttttgg taaaagtgaa gatacttttg aaatatcatt aaaaaatccc atatctaaca
      721 ttttatctgc ttcatccaaa acaaaagtat ttatattttc tagttttaaa tttccttcat
      781 ttatatgctt caatactctt ccagcagttc ctacaacaat atgagcttca tgactcaagg
      841 aaacaacttg tggtttataa ggaactcctc cacacagtgt taatactttt atatttggtg
      901 tagatcttag aacttctcta atagtttgag ctatttgagt tgctaactct cttgttggag
      961 ctaaaatcaa agattgaact ctatattttt tcttatctaa gttatttata attggtaaac
     1021 aaaaagctag agtttttcca cttcctgttt ttgctcgagc gataatatct tttttttcta
     1081 agctatattt taaactattt tcttgaatag gagttaatgt tttataaccc aaactttcaa
     1141 ggttttctat taaattctgt tctaaattta aatctctaa
//
LOCUS       DAOQAN010000504         1179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_193664_length_1179_cov_1.846085, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000504 DAOQAN010000000
VERSION     DAOQAN010000504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_193664_length_1179_cov_1.846085"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1067)
                     /locus_tag="PKO10_07375"
     CDS             complement(<1..1067)
                     /locus_tag="PKO10_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HNW67004.1"
                     /translation="MAKKHLVIDPITRIEGHLRIEAIIDENNVVVDAFSSSTMFRGIE
                     EILKGRDPRDCGLLAMRICGVCTGTHYQRSIEAVENAFSVTIPKNARLVRNLIQGALY
                     LHDHVVHFYHLHALDWVDITKALEADPKATVAEAQKWAGLSGQRAWNASEDVYIQVKE
                     RVQKYIKQGRLGIFGNAYWGSEGFKLTPEQNLIGLSHYLDALELQRDLAKMMAIFGGK
                     NPHPQSFVVGGVTCVQDIKNPARIAEFKQLLKRGRKFIKEAYLPDVYMAGTMYGEEAL
                     NGTGGGLGNYMSYGGFNLDDLPFYKSKKLFPAGIVKNKDLSKVYEVDEAKITEDVTHA
                     WYKGNTNLHPLLHLLKQHFPDL"
BASE COUNT          372 a          241 c          153 g          413 t
ORIGIN      
        1 aggtcgggaa aatgttgttt cagaagatgc agcaatggat gaagatttgt atttccttta
       61 taccaagcat gagtaacatc ttcagtaatt tttgcttcat ctacttcata aactttactt
      121 aaatctttgt tttttacaat tccagcaggg aataattttt tagatttata aaatggtaaa
      181 tcatctaaat taaatccacc ataactcata tagtttccta atccaccacc tgttccattt
      241 aatgcctctt caccatacat tgttccagcc atataaacat ctggtaaata agcctcttta
      301 ataaactttc ttcctctttt aagaagttgt ttaaattcag caattcttgc aggatttttt
      361 atatcttgaa cacaagtaac tccaccaaca acaaaagatt gaggatgagg attttttcca
      421 ccaaaaatag ccatcatttt tgctaaatct ctttgtaatt caagagcatc aagatagtga
      481 gaaagaccaa ttaagttttg ttcaggagtt aatttaaaac cttcactacc ccaataagca
      541 tttccaaaaa taccaagtct tccttgtttt atatattttt gaactctctc ttttacttga
      601 atatatacat cttcagaagc attccaagct ctttgtcctg aaagtcctgc ccatttttga
      661 gcttctgcaa cagtagcttt tgggtctgcc tcaagtgctt ttgtaatatc aacccaatca
      721 agtgcatgta gatgatagaa gtgaacaacg tggtcatgaa gatacaaagc accttgaata
      781 agatttctta ccaatctcgc atttttagga atagttacac taaaagcatt ttcaacagct
      841 tcaatacttc tttgatagtg agttcctgta caaactccac aaattctcat tgcaagcagt
      901 ccacaatctc taggatctct tccttttaaa atctcttcaa ttcctctaaa catcgttgaa
      961 gaagagaaag catcaactac aacattattt tcatctataa ttgcctcaat tctaagatgt
     1021 ccttcaattc ttgtaattgg atctattact aaatgttttt ttgccattat ttatcttccc
     1081 cttcatttga ttttttacct gcaacggctg aagctattgc gtgaattcct ataccaacag
     1141 tagcagctgt taataatcca agaccaaatt catcaacag
//
LOCUS       DAOQAN010000505         1176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_194311_length_1176_cov_3.005352, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000505 DAOQAN010000000
VERSION     DAOQAN010000505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_194311_length_1176_cov_3.005352"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            41..1162
                     /gene="tgt"
                     /locus_tag="PKO10_07380"
     CDS             41..1162
                     /gene="tgt"
                     /locus_tag="PKO10_07380"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HNW67005.1"
                     /translation="MEFQINATSQGARACTIKTAHSTILTPVFMPVGTQGTVKALDAN
                     DMLEMGAKIILGNTYHLYLRPGSKLIKKFGGLHGFSKFPNSFLTDSGGFQAFSLSDNS
                     KPDENGIMFKSHIDGSKHYFTPKSVLDTQYDLGSDIMMILDDLVALPNTKERIAKSIE
                     RTTKWAKEAIEYHMEQKSKGIGVNQNIFAIIQGGTDKEFRKLSAEQLCAMSDYDGFAI
                     GGLSVGEPNADMYETVEWTTQFMPKDKPRYLMGVGTPEDLIENIERGVDMFDCVMPTR
                     NARNGTLFTSFGRLNIKKAEFKEDIKPIDETCSCYTCKNFTRAYLNHLLRAGEITYFR
                     LASIHNIYYYLDLMKQAREAILKDNWIEFKKDFYAKRGK"
BASE COUNT          444 a          143 c          214 g          375 t
ORIGIN      
        1 atagaatata atcttaatta aaaaaaggtt tataatattt atggaatttc aaattaacgc
       61 aacttcacaa ggggctagag catgtacaat taaaacagct cacagcacta ttttaactcc
      121 tgtttttatg cctgttggta ctcaaggaac tgtaaaagct cttgatgcaa acgatatgtt
      181 agaaatgggt gcaaagatta ttttaggaaa tacttatcac ttatatttaa gacctggaag
      241 caaactaata aaaaagtttg gtggacttca tggtttttca aaatttccaa actcattttt
      301 aacagatagt ggagggtttc aagcattttc actaagtgat aatagtaaac cagatgaaaa
      361 cgggattatg tttaaatctc atatagatgg aagcaaacac tactttacac caaaaagtgt
      421 tttagatact caatatgatt tgggtagtga tattatgatg attttagatg atttagttgc
      481 cttgccaaat acaaaagaga gaattgctaa atcaattgaa agaactacaa aatgggctaa
      541 agaagctatt gaatatcaca tggaacaaaa atcaaaaggt attggggtaa atcaaaatat
      601 ctttgcaatt atacaaggtg gaacggataa agagtttaga aaactaagtg ccgaacaact
      661 ttgtgcaatg agtgattatg atggttttgc aattggtgga ttaagtgttg gtgaaccaaa
      721 tgctgatatg tacgaaactg tagagtggac tacacaattt atgccaaaag ataaacctag
      781 atatttaatg ggtgttggaa cacctgaaga tttaatagaa aatattgaac gtggtgttga
      841 tatgtttgat tgtgtaatgc ctacaagaaa tgcaagaaat ggaacacttt ttacatcttt
      901 tggaagattg aatatcaaaa aagccgaatt taaagaagat attaaaccaa tagatgaaac
      961 ttgctcttgt tatacttgta aaaatttcac acgagcttac ttaaatcatc ttttaagagc
     1021 tggtgagata acttatttta gattggcttc tatacacaat atctattact atttagattt
     1081 aatgaaacaa gctagagagg ctattttaaa agataattgg attgagttta aaaaagattt
     1141 ttatgctaaa agaggcaaat aaatcttttt tttgat
//
LOCUS       DAOQAN010000506         1175 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_194575_length_1175_cov_3.029464, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000506 DAOQAN010000000
VERSION     DAOQAN010000506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_194575_length_1175_cov_3.029464"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="PKO10_07385"
     CDS             complement(<1..291)
                     /locus_tag="PKO10_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014467936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNW67006.1"
                     /translation="MTFQDFKFKENLQKAIDEAGFKEPSPIQIDAIPHILLGKDIVGQ
                     AHTGTGKTAAFGLPVLNQLTGKNAEAVVIVPTRELAMQVSDELFKFGKFLGIN"
     gene            complement(511..>1175)
                     /locus_tag="PKO10_07390"
     CDS             complement(511..>1175)
                     /locus_tag="PKO10_07390"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014467935.1"
                     /note="an AccC homodimer forms the biotin carboxylase
                     subunit of the acetyl CoA carboxylase, an enzyme that
                     catalyzes the formation of malonyl-CoA, which in turn
                     controls the rate of fatty acid metabolism; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HNW67007.1"
                     /translation="CSLQRRHQKVIEESPAILLNDETRAKLHEVAVKATKYLKYEGAG
                     TFEFLADDKQNFYFMEMNTRLQVEHPVSEMVSGLDIIEWMIKVAEGEKLPSQDTIKFR
                     GHAIECRITAEDPNTFLPCPGKITQWMVPGGRNVRVDSHIYTNYIVPPHYDSMIGKLI
                     VWGRDREKAINIMKRALSEFEVEGIKTNIPFHKKMMENKDFISNNYDTKYLENYKGLD
                     SI"
BASE COUNT          370 a          190 c          146 g          469 t
ORIGIN      
        1 gttaattcct agaaattttc caaatttgaa taattcatcg cttacttgca ttgctaattc
       61 tcttgtagga actattacaa ctgcttctgc attttttcct gttaattgat ttaaaactgg
      121 taaaccaaat gctgctgttt taccagtacc tgtatgtgct tgaccaacta tatcttttcc
      181 taaaagtata tgtggaattg catctatttg tattggactt ggttctttaa aaccagcttc
      241 atcgattgct ttttgtaaat tttctttaaa tttaaagtct tgaaaagtca ctttttatcc
      301 ttaagtatat attgtgaata tacactctta tattaaaaaa ccttcctata atggataatc
      361 aaatataaaa aatatgtaag tttttagtat aaaattacta aatcttaaaa aaagataggc
      421 atataaatgt gtataaatct gcggagttta tctaaaatat tattagattt agctttaaga
      481 aagatttctc tttcttaaaa gaccaaatat ttagatacta tcaagacctt tgtagttttc
      541 aagatatttt gtatcgtaat tattgcttat gaaatctttg ttttccatca tttttttatg
      601 aaatggaata ttagttttaa ttccttcaac ctcaaattca cttaaagctc ttttcattat
      661 attaatagct ttttctctat ctcttcccca aactattagt tttccaatca ttgagtcata
      721 atgtggtgga actatatagt ttgtatagat atgtgaatct actcttacat ttcttcctcc
      781 tggaaccatc cattgagtta tttttccagg acatggtaaa aatgtatttg gatcttcagc
      841 tgtaattcta cactctattg catgacctct aaatttgatt gtatcttgag aaggaagttt
      901 ctcaccttcg gcaactttaa tcatccactc aataatatca agacctgaaa ccatttcaga
      961 aactggatgc tcaacttgaa gtcttgtatt catctccata aaataaaagt tttgtttatc
     1021 atcagctaag aattcaaaag ttccagcacc ttcatattta agatatttag ttgcttttac
     1081 agcaacttca tgaagttttg ctctagtttc atcatttaat aaaattgcag gtgattcttc
     1141 aataactttt tgatgtcttc tttgtaaaga acaat
//
LOCUS       DAOQAN010000507         1174 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_194872_length_1174_cov_2.647006, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000507 DAOQAN010000000
VERSION     DAOQAN010000507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_194872_length_1174_cov_2.647006"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..841)
                     /locus_tag="PKO10_07395"
     CDS             complement(<1..841)
                     /locus_tag="PKO10_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNW67008.1"
                     /translation="MNLLITRHDKIGDFVVTLPLFKAIKEQYPNTKITALVSKINYKF
                     AKDIEFIDDVILYNTNDFPRTLQDIKSKNFDASISAYIDTQLGKILYKSGIKKRVAPA
                     TKLAQFYFNKRIRQRRSKALKTEWQYNLELAKAIFPEIKLDFSKPLLNIKEKKEKRVI
                     FHTGFGGSSDGNLRIDDYINLARSIKNSTYDIVFSFGPDDEKAKEYIEANLDFEAYIY
                     ESKVTLMDFARYIASSSLFVSTSTGPMHLAGASNSQTLSFFGDSLFASSKRWATVSDE
                     KYQN"
     gene            complement(838..>1174)
                     /locus_tag="PKO10_07400"
     CDS             complement(838..>1174)
                     /locus_tag="PKO10_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014469180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNW67009.1"
                     /translation="NSNDVHEDIVTDGLNIEELKGNVEHYSYNTISEFIIKADRYSTL
                     FASNNAGKKSSSPVKAFFNASYSFFRTYILKQGFRDGYVGLVIAFSHMVTNFYKYIKL
                     YQLNKELKK"
BASE COUNT          391 a          183 c          132 g          468 t
ORIGIN      
        1 tattttgata tttttcatca cttactgttg cccatctttt actacttgca aataagctat
       61 ctccaaaaaa agagagagtt tgagaatttg aagcacctgc aagatgcata ggtcctgttg
      121 aagtactcac aaaaagagaa ctacttgcta tatatcttgc aaaatccatt aaagtaactt
      181 ttgactcata aatatatgct tcaaaatcta aattagcctc aatatactct tttgctttct
      241 catcatcagg accaaaagag aaaactatat cataagtgct atttttgata cttctagcca
      301 agtttatata atcatcaatt cttaagtttc catcactact accaccaaat ccagtatgaa
      361 aaataactct tttctctttt ttctctttaa tatttaaaag tggttttgaa aaatcaagct
      421 taatttctgg aaaaattgct tttgctaatt caagattgta ttgccactct gtttttaaag
      481 ctttacttct tctttgtctt atacgtttgt tgaagtaaaa ttgagctaat tttgttgctg
      541 gtgcaactct ttttttaatt ccgcttttat aaagtatttt tcctaattgt gtatctatat
      601 atgcactaat acttgcatca aaatttttag attttatatc ttgtaaagtt ctaggaaaat
      661 catttgtatt atataaaata acatcatcta taaactcaat atcttttgca aatttataat
      721 ttatttttga aaccaaagct gtaatttttg tattaggata ctgctctttt atagctttaa
      781 aaagtggtaa agtaactaca aaatccccta ttttatcgtg tcttgttata agtaaattca
      841 tttttttaac tctttattta attggtataa ttttatatat ttataaaaat ttgtaaccat
      901 atgagaaaag gcaattacaa gtcctacata accatctcta aaaccttgtt ttaagatata
      961 tgttctaaaa aatgaataac ttgcattaaa aaaagctttt actggacttg aagatttttt
     1021 tcccgcatta ttagatgcaa aaagtgttga gtatctgtct gctttaatta taaattctga
     1081 gattgtatta taactataat gctcaacatt tccttttaac tcttctatat ttaaaccatc
     1141 agttacaata tcctcatgaa catcatttga gtta
//
LOCUS       DAOQAN010000508         1174 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_194959_length_1174_cov_1.937444, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000508 DAOQAN010000000
VERSION     DAOQAN010000508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_194959_length_1174_cov_1.937444"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="PKO10_07405"
     CDS             complement(<1..439)
                     /locus_tag="PKO10_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNW67010.1"
                     /translation="MFKKSIKAYKEACEVIPGGVDSPVRAFKSVGGTPPFIKKGKGAY
                     LYDIDGNKYLDFVQSWGPLIFGHCDKDIEKAVIKTAKLGLSFGAPTNLETKLASEIVE
                     MYENIDKVRFVSSGTEATMSAIRLARGVTNKNDIIKFEGCYHGH"
     gene            565..>1174
                     /locus_tag="PKO10_07410"
     CDS             565..>1174
                     /locus_tag="PKO10_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW67011.1"
                     /translation="MKKIVLAFVVSLLIHILLFLNYSFKESAKEETKKDNEKSEVKFV
                     KLVQEKVLQEQTAKSVEPKIDEPKIVEQKQQNIKTPKIVEKEQKASSELKANNSKKQL
                     DIKEAKKVQKEILKESSNLQDNTLEKFLSQKTPINQEVLNELQKLYGKEYDNFTNVQK
                     AYLEKNLNNFQVITQKVLNRMGYPKLAAKLGIGGINTIEFIFH"
BASE COUNT          476 a          166 c          140 g          392 t
ORIGIN      
        1 agtgtccatg ataacaaccc tcaaatttta taatatcatt tttatttgta acacctcgtg
       61 ccaatcttat tgcactcata gtagcttctg ttccagagct tacaaatctt actttatcaa
      121 tattttcata catttcaaca atctcagaag caagttttgt ttcaagattc gttggagctc
      181 caaaactcaa acctaacttt gcagttttta taactgcttt ttcaatatct ttatcacaat
      241 gtccaaaaat aagtggtccc caactttgta caaaatctaa atatttgtta ccatcaatat
      301 catataaata ggcaccttta ccttttttaa taaatggtgg tgttccacct acgcttttaa
      361 atgctcgaac aggagaatca acacctcctg gaattacttc acaagcttct ttgtaggctt
      421 ttatagattt tttaaacata tttttttcct aaattataat taagttttta ttctactata
      481 atatttataa gcaaaaggtt atttatatat ttatcaaatt taaaaataaa tacaattttt
      541 aaattttttt actataattc gcgcatgaaa aaaatagtac ttgcatttgt cgtttcttta
      601 ttgattcata ttttactttt tttaaattat agctttaaag agtctgctaa agaagagaca
      661 aaaaaagata atgaaaaaag tgaagtaaaa tttgtaaaat tagtacaaga aaaagtattg
      721 caagaacaaa cagcaaaaag tgtcgagcct aaaatagatg aacccaaaat tgttgaacaa
      781 aaacaacaaa atataaaaac tccaaaaata gttgaaaaag agcaaaaagc aagtagtgaa
      841 ttaaaagcta acaatagtaa aaaacaacta gatataaaag aggctaaaaa agtccaaaaa
      901 gagattttaa aagagagtag taatcttcaa gataatactt tagaaaaatt tttatctcaa
      961 aaaactccta taaatcaaga agttttaaat gaacttcaaa aattatatgg aaaagagtat
     1021 gataatttta caaatgtaca aaaagcctat ttagaaaaaa atctaaataa ttttcaagta
     1081 attactcaaa aagttttaaa tcgtatgggt tatccaaaat tagctgcaaa gcttggaatt
     1141 ggtggaataa atactataga gtttatattt catc
//
LOCUS       DAOQAN010000509         1173 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_195247_length_1173_cov_1.641324, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000509 DAOQAN010000000
VERSION     DAOQAN010000509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_195247_length_1173_cov_1.641324"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(162..785)
                     /locus_tag="PKO10_07415"
     CDS             complement(162..785)
                     /locus_tag="PKO10_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013135530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HNW67012.1"
                     /translation="MEIVKNSSIVIVNSIEDSLNKFLAVYPTHQTRVIKNEEKDEFQI
                     EQANKTLKEAYIASNETKYLFLCGATFRVEAQNALLKILEEPPKNIVFILLVSSKNSL
                     LPTIYSRLPYKNLKKVVEKDDIELNIKKLDLKDIYTFIKNNQKITKDEAINIVETILI
                     KANKENVKLNQKELDIFFKSIKLLELNSKPTTVLTYLLLSILEKEAK"
     gene            complement(788..>1173)
                     /locus_tag="PKO10_07420"
     CDS             complement(788..>1173)
                     /locus_tag="PKO10_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HobA family DNA replication regulator"
                     /protein_id="HNW67013.1"
                     /translation="WFLNYILSNINSSKLNRPFLPFYDFSSFYKNLDQIKSEDDISNI
                     KDMLKISFPNGYCFWYIGKSQDNRSTLAKITKSSFLWIFDEERQGSINLKSNDDGLDM
                     KLLQMFRLYNKTISAALFANINVES"
BASE COUNT          390 a          146 c          145 g          492 t
ORIGIN      
        1 taaagcatct tgctttaata tattagcagc acctacatgc aaatctttta taaaaagtgt
       61 atgaattttt gctttttttg acataatagc aacaccactt ttatcacatt ttagagtgct
      121 taaaaatgtt tttgtctcat ttaaagatag tttatatagt ttcattttgc ctctttttct
      181 aaaatagata gaagaagata agttaaaact gtagttggtt ttgagtttag ttccaaaagt
      241 tttatagatt taaaaaatat atccaactct ttttggttaa gtttgacatt ttctttattt
      301 gcttttatta agatagtttc aacaatattt atagcttcat cttttgtaat tttttgattg
      361 ttttttataa aagtgtatat atcttttaaa tctaattttt ttatatttag ttcaatatca
      421 tctttttcca caactttttt aagattttta tatggtaatc ttgaataaat tgtaggaagt
      481 agtgaatttt tagaagagac taaaagaata aaaactatat tctttggtgg ttcttctaaa
      541 atttttaaaa gagcattttg tgcttcaact ctaaatgtag caccacacaa aaatagatat
      601 tttgtctcat ttgatgcaat atatgcctct tttagagttt tatttgcttg ttctatttgg
      661 aactcatctt tctcttcatt tttaataact ctagtttgat gagtagggta aacagctaga
      721 aatttattta agctatcttc aatgctattt acaataacaa ttgaagagtt tttaactatc
      781 tccaagatta gctctctaca ttaatatttg caaaaagtgc ggcacttatt gttttgttat
      841 ataatctaaa catttgaaga agcttcatat caagtccatc atcatttgat tttaagttta
      901 tagaaccttg tctctcctca tcaaaaatcc ataaaaaaga gctttttgtg atttttgcaa
      961 gagtagatct gttatcttga ctttttccaa tataccaaaa acagtatcca tttggaaaag
     1021 atatttttag catatcttta atatttgaaa tatcatcctc tgattttatt tgatcaagat
     1081 ttttataaaa tgaactaaaa tcataaaatg gtaaaaaagg tctatttagt tttgaagagt
     1141 ttatgttaga taaaatatag tttaaaaacc att
//
LOCUS       DAOQAN010000510         1172 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_195430_length_1172_cov_2.332140, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000510 DAOQAN010000000
VERSION     DAOQAN010000510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1172
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_195430_length_1172_cov_2.332140"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /gene="mnmE"
                     /locus_tag="PKO10_07425"
     CDS             complement(<1..858)
                     /gene="mnmE"
                     /locus_tag="PKO10_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008335110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HNW67014.1"
                     /translation="MYNDDTIVAIATASGIGSISIVRVSGAKALEIALKISQKTTINP
                     RIATLSYLYDKKQNIIDEAIVLYFKSPNSFTGEDIVEFQIHGGVAIASLVLDTVLEYG
                     ARMATAGEFSKRAFLNNKIDLSKAEAISKIIEARSSDAVKLLARQLKGELKDFVEDIR
                     EDLLFMLAYTEVTIDYAEEDLPSDIFSKIEEKISKIEQKLENTLEASKRREGMIDGFK
                     VAIIGKPNVGKSSLLNKLLNYDRAIISDIAGTTRDTIEESVKIGTHIIKIVDTAGIRR
                     KAKVKEDLEF"
     gene            complement(861..>1172)
                     /locus_tag="PKO10_07430"
     CDS             complement(861..>1172)
                     /locus_tag="PKO10_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HNW67015.1"
                     /translation="GYRYKALSYILFNWINDKFGLMLRLEVAEFLKNQEAAICLYLEP
                     VIEIIKEKGSFKTKPLDGILVHIALKRLREEFPLKYVAVKTNVKGEKYVLVNEYKQKE
                     D"
BASE COUNT          366 a          220 c          128 g          458 t
ORIGIN      
        1 aaattctaaa tcttctttta cttttgcttt acgtctaatt ccagctgtat ctacaatttt
       61 tataatatgt gtacctattt ttacactctc ttcaattgta tctcttgtag ttcctgcaat
      121 atcagaaata atagctctat catagttaag taatttattt aaaagactag attttcctac
      181 atttggtttt ccaataattg caactttaaa cccatcaatc ataccttctc ttcttttact
      241 agcttctaga gtattttcta gtttttgttc aatttttgat atcttttctt ctatttttga
      301 aaagatatca ctaggtagat cttcttcagc ataatcaatc gtaacctctg tatatgcaag
      361 cataaatagc aaatcttctc taatatcttc tacaaaatct ttaagttcac cctttaactg
      421 tctagccaat agttttacag catcactact tcttgcttca attatttttg atattgcttc
      481 tgctttactc aaatctattt tattattcaa aaaagctctt tttgaaaact ctccagcagt
      541 tgccattcta gcgccatact ctaaaactgt atccaatact agtgaagcaa tagcaactcc
      601 accatgaatt tgaaactcaa ctatatcttc tcctgtaaaa gagtttggag atttaaaata
      661 taagacaatt gcttcatcaa taatattttg ttttttatca tacaaataag atagagttgc
      721 aattcttgga tttatagtag ttttttgtga gattttaaga gcaatttcaa gagcttttgc
      781 tcctgaaact ctaactattg aaatagaacc tattccagat gctgtagcaa ttgctacaat
      841 agtatcatcg ttatacaata ttaatcttct ttttgtttgt actcatttac aagtacatat
      901 ttttcacctt ttacatttgt tttaacagca acatatttca aaggaaactc ttctcttaat
      961 cttttaagag caatatgcac taaaatacca tcaagaggct ttgttttaaa actccccttc
     1021 tctttgatta tctcaattac aggctctaag tatagacaaa tagcagcttc ttgatttttt
     1081 aaaaattcag caacctcaag gcgaagcatc aatccaaatt tatcatttat ccagttaaat
     1141 aagatatacg ataaagcctt gtatctgtag cc
//
LOCUS       DAOQAN010000511         1172 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_195440_length_1172_cov_2.256938, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000511 DAOQAN010000000
VERSION     DAOQAN010000511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1172
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_195440_length_1172_cov_2.256938"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="PKO10_07435"
     CDS             <1..200
                     /locus_tag="PKO10_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW67016.1"
                     /translation="GILSIIKFYKALNSIGIEYNVPLMQLVAKGYIVGIVLVPLFGLG
                     YLILFVAFIAKIFAYLKIEKQ"
     gene            210..617
                     /locus_tag="PKO10_07440"
     CDS             210..617
                     /locus_tag="PKO10_07440"
                     /inference="COORDINATES: protein motif:HMM:NF024276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HNW67017.1"
                     /translation="MKNIFKLIFITIIGLLVLAGCSDNSPKGATLAFLNAMKDGNVED
                     FRKYSTQSTQQVMAFALAMQCKGAIKEDSALSTCLKSVTKDVSKYKVVDVQENGKTSA
                     IVTAEIYGKDGNINKESYNLEKFADEWKINISK"
     gene            617..>1172
                     /locus_tag="PKO10_07445"
     CDS             617..>1172
                     /locus_tag="PKO10_07445"
                     /inference="COORDINATES: protein motif:HMM:NF017519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="HNW67018.1"
                     /translation="MKNKLFLSLIVASLLIYLASNLFEKKISKKENQQSFDLNIIKSG
                     DIIFRKERNFLSNMFSNIDKSKYSHIAIALKVGENLKIYHIESNSEKDDLKIDTFEKF
                     TKFASKIAVYRYNKEIDSEKIIQILEEYEKNKIEFDIDFELENDKLYCTELINDIYLK
                     LFNENIYSYLYQFYGKQGITINGIL"
BASE COUNT          500 a          101 c          174 g          397 t
ORIGIN      
        1 ttggaattct ttctattata aaattttata aagctttaaa ctctattgga attgaatata
       61 atgtaccttt aatgcaacta gttgcaaaag gttatattgt tggtattgtt ttagttccac
      121 tatttggttt aggatattta atactttttg tggcatttat tgcaaaaata tttgcttatt
      181 taaaaataga gaaacaataa ggaaaaaaaa tgaaaaatat atttaaatta atatttataa
      241 caattattgg attgttagtt ttagcaggat gcagtgataa tagtccaaaa ggtgcaacat
      301 tagcattctt aaatgctatg aaagatggaa atgttgaaga ttttagaaaa tattcaactc
      361 aatctactca acaagttatg gcatttgctc tagctatgca atgtaaaggt gctataaaag
      421 aagatagtgc attatcaact tgtctaaaaa gtgtaactaa agatgtttca aaatataaag
      481 ttgttgatgt tcaagaaaat ggaaaaactt cagctatcgt aacagcagaa atttatggaa
      541 aagatggaaa tataaataaa gagagttaca atttagaaaa atttgcagat gagtggaaga
      601 taaatataag taaatagtga aaaataaact atttttatct cttatagttg ctagcttatt
      661 aatatattta gctagtaact tatttgaaaa aaagattagt aaaaaagaaa atcaacaatc
      721 tttcgactta aatattataa aaagtggaga tataatattt agaaaagaga gaaattttct
      781 aagtaatatg ttttccaata ttgataagtc aaaatattct catattgcaa ttgcacttaa
      841 agttggtgaa aatctaaaaa tatatcatat tgaatcaaat agtgaaaaag atgatttaaa
      901 aatagatact tttgaaaaat ttacaaaatt tgcttcaaaa atagcagttt atagatataa
      961 caaagaaatt gattcagaaa aaataataca aattcttgaa gagtatgaaa aaaacaaaat
     1021 agagtttgat attgactttg agttagaaaa tgataaatta tattgcacag agctaatcaa
     1081 tgatatatat ttaaaactgt ttaatgaaaa tatatatagc tatttgtatc agttttatgg
     1141 aaaacaggga attacaataa atggaatact ta
//
LOCUS       DAOQAN010000512         1166 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_196903_length_1166_cov_3.070207, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000512 DAOQAN010000000
VERSION     DAOQAN010000512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_196903_length_1166_cov_3.070207"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="PKO10_07450"
     CDS             complement(<1..273)
                     /locus_tag="PKO10_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014473359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNW67019.1"
                     /translation="MKILLLEDELMLNSAISEYLKNIGHMVESFFDGAEVLKSIENGY
                     DLLILDVNVPNVDGFSILNELNNRKIHIPTIFISAQIDIEDITKAYN"
     gene            complement(254..>1166)
                     /locus_tag="PKO10_07455"
     CDS             complement(254..>1166)
                     /locus_tag="PKO10_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNW67020.1"
                     /translation="VQHNEDVTQEQIVNDVKEIGKYFKESEERNEPINQNRITLGEFK
                     IIANYANDTMTNIKLKSSMLEELNKNLEIKVNEKTKELSDLVESQKQFLKNSVHEINT
                     PLAIIRTNIDLLKMKIPENKYITNIESGSKTIQYIYDDLSYLIKKDRVLYEKEYLEFS
                     EILKNRLEFFEEIVKSNNLYFIKNIEDDVYLKFNSVELQRIIDNNISNAVKYSFSKSA
                     IHIKLYYINDNEIEFSVTTNSKIIEDTNKIFEDFYRENKARGGFGLGLKIVKEICDKN
                     LVTIEILSDKKETKFSYRFKINEDTTT"
BASE COUNT          380 a          164 c           99 g          523 t
ORIGIN      
        1 attataagct tttgtaatat cttcaatatc aatttgtgct gatataaaaa ttgtaggaat
       61 atgaattttt ctattattta gttcatttaa aatagaaaaa ccatctacat ttggtacatt
      121 aacatcaaga attagtaaat cataaccatt ttcaatactt tttaaaactt cggcaccatc
      181 aaaaaaactt tcaaccatat gaccaatatt ttttaaatat tcgctaatgg cactatttaa
      241 catcaattca tcttcaagta gtagtatctt catttatttt aaacctataa ctaaattttg
      301 tctctttttt atctgataat atttcaattg taactaaatt tttatcacaa atctctttta
      361 caatttttaa tcctaatcca aatccaccac gagctttgtt ctctctataa aaatcttcaa
      421 agattttatt tgtatcttct attatttttg agtttgttgt tacactaaac tctatttcat
      481 tgtcatttat ataatataac tttatatgaa ttgctgattt actaaatgaa tattttacag
      541 catttgaaat attattatct ataattcttt gaagttctac agagttaaat tttaaataga
      601 catcatcttc aatatttttt ataaaatata agttatttga ttttacaatc tcttcaaaaa
      661 attctaatct attttttaga atttctgaaa actctaaata ctctttttca tataaaactc
      721 tatctttttt tataagatat gataaatcat cataaatata ttggatagtt tttgaaccag
      781 attcaatatt tgtaatatat ttattttcag ggattttcat ctttagcaaa tcaatatttg
      841 ttcttataat tgctaaagga gtattaattt catgcacaga attttttaaa aattgttttt
      901 gagattctac taagtctgat agctcttttg ttttttcatt tacttttatt tctaaatttt
      961 tatttagctc ttcaagcata gagctcttta attttatgtt tgtcatagta tcatttgcat
     1021 aatttgcaat aatcttaaac tctcctaagg taatcctatt ttgattgatt ggctcatttc
     1081 tttcttcaga ttctttaaaa tatttaccta tctcttttac atcatttact atttgttctt
     1141 gtgttacatc ttcattatgt tgaact
//
LOCUS       DAOQAN010000513         1165 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_197221_length_1165_cov_2.640541, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000513 DAOQAN010000000
VERSION     DAOQAN010000513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_197221_length_1165_cov_2.640541"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            152..>1165
                     /locus_tag="PKO10_07460"
     CDS             152..>1165
                     /locus_tag="PKO10_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012011955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HNW67021.1"
                     /translation="MDIKTIKTIESATEKSISSFAKLSLSLLFLLAVFLWTYSSHGKV
                     PDNTFLIIGAIFGAYMALNIGANDVANNVGPSVGAKALTLTGAIIIAGIFESAGAIIA
                     GGDVVNTIKSGIIDPNLIADKNSFIWAMTAGLLAGAVWLNFATSIGAPVSTTHAIVGG
                     VMGAGIASAGFSILNWGSLAEIASSWIISPLLGGIVAAGFLYFIKKQIVYKENMLEQA
                     QKFVPILIAIMTWAFTTYLILKGLKQLINIGFYSASGISILFAIVAYYLTKKSIEKNL
                     SNLTNDRSSVNKLFTPALIFGAALLSFAHGANDVSNAIGPLAAINDAIMHGGTSVQAS
                     VPFW"
BASE COUNT          390 a          156 c          200 g          419 t
ORIGIN      
        1 ctgaatctaa aaaaggaaaa ttaaatctta atattattta atgttaaggt ataatttatc
       61 ttcttttcat ttttacttat aactaaactt taaataagat taattaaact ataatgactt
      121 cattacaatt tagttacata aggaaaataa tatggatatt aagaccataa aaaccataga
      181 aagtgccacc gaaaaatcta tctcaagctt tgcaaaatta tctctctctt tgctattttt
      241 attagctgta tttctttgga catattcatc tcacggtaaa gtacccgaca atactttttt
      301 aataattggt gctatttttg gcgcttatat ggctttaaat attggagcaa atgatgttgc
      361 aaataatgta ggaccatcag ttggagcaaa agctttaacc ttaacaggtg caattataat
      421 agcaggaata tttgaaagtg ctggagcaat tatagcaggg ggtgatgttg taaatacaat
      481 caaaagtgga attatagacc caaatttaat tgcagataaa aattcattta tttgggctat
      541 gacagctgga cttcttgctg gtgctgtttg gttaaatttt gcaacatcta ttggagcacc
      601 agtttcaaca acacatgcaa tagttggtgg agtaatggga gctggaattg cgagtgctgg
      661 tttttctatt ttaaattggg gtagtttagc agaaattgca tcttcgtgga ttatatctcc
      721 tttactaggt ggtattgttg cagctggttt cttatatttc ataaaaaaac agattgttta
      781 caaagagaat atgcttgaac aagcgcaaaa atttgttcct attttaattg caataatgac
      841 ttgggcattt acaacttatc ttattctaaa aggtttaaaa cagttaataa atataggatt
      901 ttatagtgct tctggaataa gtattttatt tgctattgta gcttattatc ttactaaaaa
      961 aagtattgaa aagaatttat caaatttaac aaatgataga tcaagtgtta ataaactttt
     1021 tacacctgct ttaatatttg gtgcagcact attatcattt gcacatggtg ctaatgatgt
     1081 ttcaaatgct attggaccgc ttgctgctat aaatgatgca attatgcatg gaggtacaag
     1141 tgtacaagca tctgtaccat tttgg
//
LOCUS       DAOQAN010000514         1164 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_197414_length_1164_cov_4.435528, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000514 DAOQAN010000000
VERSION     DAOQAN010000514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1164
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_197414_length_1164_cov_4.435528"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(165..935)
                     /gene="pstB"
                     /locus_tag="PKO10_07465"
     CDS             complement(165..935)
                     /gene="pstB"
                     /locus_tag="PKO10_07465"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HNW67022.1"
                     /translation="MNKDIKTKIDVKNLNLFYGSNQALFDIDVNLYQNKITALIGPSG
                     CGKSTFLRCINRMNDLIPIVKIDGKIIIDKKNIYDKDVDEVSVRKRVGMVFQQPNPFP
                     KSIYDNVAYAPLKHGMVKKGKECDELVETSLIKAGLWNEVKDKLTTPGTSLSGGQQQR
                     LCIARTIAIKPEIILMDEPTSALDPISTEKIEALMLELKQDYTIITVTHNMQQAARVA
                     DYTAFFHLGKLIEYDVTETIFVNPTNKKTEDYITGRFG"
     gene            complement(950..>1164)
                     /locus_tag="PKO10_07470"
     CDS             complement(950..>1164)
                     /locus_tag="PKO10_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014467968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate ABC transporter, permease protein
                     PstA"
                     /protein_id="HNW67023.1"
                     /translation="IIGMIAFIPDAPSMVTGAATVMPAQLFTWAGMPEGMYIEKTAAG
                     ILVLLTILISLNAIAIILRKKLEVKW"
BASE COUNT          351 a          200 c          131 g          482 t
ORIGIN      
        1 ttttactaga aatttttttt cactaacttc aacatttttt agattctcta tttttctctc
       61 atttaaagct tttaaacaaa cctctaaagc ctcaacaata tctaatccta gtttttctac
      121 ttcacttttt ataagtttta atttctcttc atatggtttt aacattatcc aaatctccct
      181 gtaatataat cttctgtttt tttatttgtt ggattaacaa agatagtctc tgttacatca
      241 tactctatta gttttcctaa gtggaaaaat gctgtataat ctgcaactcg tgctgcttgt
      301 tgcatattat gagttaccgt aattattgta taatcttgtt ttaactcaag cataagtgct
      361 tcaatttttt ctgtagaaat tggatctagt gctgaagttg gctcatccat taaaataatt
      421 tctggtttta tagcaattgt tctagctata caaagtcttt gttgctgacc acctgaaagt
      481 gatgttccag gagttgttaa tttatctttt acttcattcc aaagtccagc ttttattaaa
      541 gatgtttcaa ccaactcatc acactctttt ccttttttta ccataccatg ttttaaaggt
      601 gcataagcaa cattatcata aattgatttt gggaaaggat ttggttgttg aaataccatt
      661 ccaactcttt tcctaacact tacttcatct acatctttat catagatatt tttcttatca
      721 attataattt ttccatcaat tttaacaata ggaattaaat cattcattct atttatacat
      781 cttaaaaatg tagattttcc acaacctgat ggtccaatta atgctgtaat tttattttga
      841 tataaattaa catctatatc aaacaaagct tgatttgagc cataaaaaag atttagattt
      901 ttaacatcta tttttgtttt tatatcttta ttcatttaat attttccttt taccatttta
      961 cttcaagttt ttttcgcaag attatagcta ttgcatttag tgaaattaat attgttaata
     1021 aaactaaaat tcctgctgct gttttttcta tatacatacc ttcaggcatt ccagcccaag
     1081 taaatagttg tgcaggcata actgttgccg ccccagtaac cattgaagga gcatcaggaa
     1141 taaatgcaat cattcctata ataa
//
LOCUS       DAOQAN010000515         1163 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_197712_length_1163_cov_3.249097, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000515 DAOQAN010000000
VERSION     DAOQAN010000515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1163
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_197712_length_1163_cov_3.249097"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="PKO10_07475"
     CDS             complement(<1..581)
                     /locus_tag="PKO10_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014469023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HNW67024.1"
                     /translation="MKKTVLLSSILLTSIFAFDLKSIATEVGKNIPSTTNQSQNKSNL
                     DNSTISSGLKEALKSGVTFATTQLGKKDGYLNNKDVRIPLPPNLANAETLIRKAGGDK
                     MADDLIKSMNSAASQAAPKTADIFMDAISKMSLTDAQKILNSGENGATDYFKKNTTDS
                     LKKMIKPIIQSSMKDNNVAQYYDMANSFYQSSA"
     gene            695..>1163
                     /locus_tag="PKO10_07480"
     CDS             695..>1163
                     /locus_tag="PKO10_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012013178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HNW67025.1"
                     /translation="MEQFIQDWGYIALFLYSFGGGFLALAIAGAFSFDGTLNIYITVI
                     VAAVANIVGSQFLFFMAKNNKNYAQNMMNNHKRKVALVQLLFRKYGFFVIIIQKYIYG
                     IKTLVPLVMGLSDYSSFKFTVINSIASILWACVIGYASYIAGSFLLEYADNFKY"
BASE COUNT          368 a          140 c          171 g          484 t
ORIGIN      
        1 tttgcacttg attgataaaa actgtttgcc atatcgtaat attgtgcaac attgttatct
       61 ttcatactag attgaataat tggtttaatc atttttttca aagagtctgt tgtatttttt
      121 ttgaaataat ctgttgctcc attttctcca ctatttagaa ttttttgtgc atcagttaaa
      181 ctcatttttg aaatagcatc cataaaaata tcagcagttt ttggagcagc ttgcgatgca
      241 gcactgttca tagatttaat taaatcatca gccattttat ctccaccagc ttttcttata
      301 agcgtttcag catttgctag attaggagga agaggaattc ttacatcttt attgtttaaa
      361 taaccatctt tttttccaag ttgagttgta gcaaatgtta caccactttt taaagcctct
      421 tttaaaccgc tagaaattgt actattgtct aagtttgatt tattttgtga ttgattagta
      481 gttgaaggta tattttttcc aacttctgtt gcaatactct ttaaatcaaa agcaaaaata
      541 gatgttaata agattgatga tagcaaaact gtttttttca taaagtctcc taaataaatt
      601 tttttattat ataaaattta tactaaattt agattatagc tataatcaaa caagatttag
      661 ataaaataaa aaattagtaa aacataggaa agttatggaa cagtttattc aagattgggg
      721 ctatatagct ctttttttat attcatttgg tggtggtttt ttggctcttg caattgctgg
      781 agctttttca tttgatggta ctttaaatat ttatattaca gttattgttg ctgcagttgc
      841 aaatattgtt ggtagtcagt ttctgttttt tatggcaaaa aataataaaa attatgcaca
      901 aaatatgatg aataatcata aaagaaaagt tgcacttgta caacttttat ttagaaaata
      961 tggctttttt gtaataatta ttcaaaaata tatttatgga attaaaactt tagttcctct
     1021 tgttatgggt ttgtctgatt atagttcatt taaatttact gttattaact caatagcttc
     1081 tattttatgg gcttgtgtta taggatatgc tagttatatt gcaggttcat ttttactaga
     1141 atatgctgat aattttaaat atg
//
LOCUS       DAOQAN010000516         1163 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_197750_length_1163_cov_2.789711, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000516 DAOQAN010000000
VERSION     DAOQAN010000516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1163
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_197750_length_1163_cov_2.789711"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..749
                     /locus_tag="PKO10_07485"
     CDS             <1..749
                     /locus_tag="PKO10_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNW67026.1"
                     /translation="NSLALISDAIHMFTHSFALIISLFAIIIASKTAPISKTFGYYRA
                     EVLAAFINGITIVLSIIWIIYEAIERFLNPQIIDIKTAMIVAIIGLFVNIITGVILMQ
                     GDKDNINLKSAFIHMLSDALSSVAIIIGYIVIYFTSWYFVDIILALIVSLVILKWAID
                     ILKNSTNTLLESSPVDVKEVQEYIEKNEKVLELHDIHIWEITQDMYNMTAHIKIDKKD
                     LENYEEILHQINHNLKEKFKIVHTTFQFEW"
     gene            complement(778..>1163)
                     /locus_tag="PKO10_07490"
     CDS             complement(778..>1163)
                     /locus_tag="PKO10_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter"
                     /protein_id="HNW67027.1"
                     /translation="NPFAAKFMPTMFILIAPPAIGFISYIKLTGNLDFFAQILFSLAL
                     FFTILVAFMYKNFVKIKFFISWWAFTFPLAAVTLSSILMYELTKKDFYMFLSYTLAMI
                     TTLIVILVAVATIKHMAKKEICIME"
BASE COUNT          447 a          139 c          151 g          426 t
ORIGIN      
        1 caaactctct tgctcttatt tcagatgcaa ttcatatgtt tactcactct tttgctttga
       61 ttatttcact atttgcaata ataattgcaa gtaaaacagc accaatttct aaaacatttg
      121 gttattatag agctgaggta ttagctgcat ttataaatgg aattactatc gtattatcaa
      181 ttatttggat tatttatgaa gctattgaaa gatttttaaa tccacaaatt attgacataa
      241 aaacagcaat gatagttgca attattggtc tttttgtaaa tattatcact ggtgttattt
      301 tgatgcaagg tgataaagat aatataaatc taaaatctgc atttattcat atgctaagcg
      361 atgccttatc ttcagttgca attatcatag gttatatagt tatctatttt acttcatggt
      421 attttgtaga tattatttta gctcttatag tctctcttgt aattttaaaa tgggcaattg
      481 atattttgaa aaattcaaca aatactttac ttgaaagctc tcctgttgat gtaaaagaag
      541 ttcaagagta tattgaaaaa aacgaaaaag ttttggaatt acatgatata cacatttggg
      601 agattaccca agatatgtat aatatgactg ctcatataaa aatagataaa aaagatttag
      661 aaaattatga agagatttta catcaaataa atcataattt aaaagaaaaa tttaaaatag
      721 ttcatacaac ttttcagttt gagtggtaag tttttataaa taatctcttg tataaatcta
      781 ttccattatg catatctctt tttttgccat atgttttatc gtagcaactg caaccaaaat
      841 tacaattaaa gttgtaatca ttgctagagt ataagataaa aacatataaa aatctttttt
      901 tgttaattca tacattaaaa tagaacttaa agttacagct gctaaaggaa aagtaaaagc
      961 ccaccatgag ataaaaaatt ttatttttac aaaattttta tacataaatg caactaaaat
     1021 agtaaaaaat agagctaaac taaataaaat ttgggcaaaa aaatctaaat ttccagttaa
     1081 ttttatatat gagataaatc ctattgcagg aggtgctatt aaaataaaca ttgttggcat
     1141 aaatttagca gcaaatggat tat
//
LOCUS       DAOQAN010000517         1162 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_198152_length_1162_cov_1.792231, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000517 DAOQAN010000000
VERSION     DAOQAN010000517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_198152_length_1162_cov_1.792231"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(62..>1162)
                     /locus_tag="PKO10_07495"
     CDS             complement(62..>1162)
                     /locus_tag="PKO10_07495"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HNW67028.1"
                     /translation="LAEGPLLARIGFLRESFEYPELQNDSKLKERASSPLELFFDLML
                     VAILYLGVAYHSEFGVWSFAYDKLMQISILDYNTKVWLFLGFLVAFAIKIPLFPLHTW
                     IMETYKNAPTGAVFLLSSIMAKLGVYAVLRFMIPLFPEVYIEFSTWFVIIGLFGLVYF
                     GIAALMQDDIKRMFAYSSASHLSFIAAGIFSLNSYGINGALYLIIAHAIATGALFLLV
                     GLMQEQTGFKTIKDLGGIAKKAPIFTFVFAVMLFANVGLPGTNGFVSELLIIFGVFNF
                     NIYLGVIAALSVIIGASYMLWMFQRAILQDRPEGSAELNMRDLKIKEIVGLVPWVVLV
                     FIMGFYPEVFIDKFEPTVTHYLQDILQIGATR"
BASE COUNT          459 a          204 c          157 g          342 t
ORIGIN      
        1 aacagaagag caatagcccc aaataaaacc agtaaagttg gaattaaata aataaaatta
       61 ctcatcttgt tgctccaatt tgtaaaatat cttgtaagta gtgtgtaact gttggttcaa
      121 atttatctat aaaaacttct ggataaaatc ccataataaa cactaaaaca acccatggaa
      181 caagtccaac aatctctttg atttttaaat ctctcatatt aagttcagct gaaccttctg
      241 gtctatcttg caaaattgct ctttgaaaca tccaaagcat ataagaagct ccaataataa
      301 ctgataaagc agcaataaca cctagataaa tattaaagtt aaatacacca aaaattataa
      361 gtaattcaga aacaaatcca tttgtacctg gaagtccaac atttgcaaaa agcataacag
      421 caaaaacgaa tgtaaatata ggagcttttt ttgcaattcc acctaaatct tttatagttt
      481 taaatcctgt ttgttcttgc attaatccta caagtaaaaa tagggctcct gtagcaattg
      541 cgtgagcaat aattaaatat aaagctccat ttataccata agagtttaac gaaaaaatac
      601 cagcagctat aaaacttaag tgtgaagctg aagagtatgc aaacattctt ttaatatcat
      661 cttgcataag tgcagctatt ccaaagtaca caagaccaaa tagaccaata attacaaacc
      721 aagttgaaaa ctcaatataa acctctggaa atagtggtat cataaatctt aaaacagcat
      781 aaactcctag ttttgccata atcgatgata gtaaaaatac agcaccagtt ggagcatttt
      841 tatatgtttc cataatccaa gtatgaagag gaaatagtgg aatttttatt gcaaatgcta
      901 ctaaaaatcc taaaaataac caaacttttg tattgtaatc aagtattgat atttgcatta
      961 atttatcata agcaaaagac caaacaccaa actcactatg ataagcaact cctagatata
     1021 aaattgctac aagcattaaa tcaaagaata gttcaagtgg tgacgaggct ctctctttca
     1081 atttactatc attttgaagt tctggatatt caaagctttc tcttaagaaa ccgatcctag
     1141 caagaagggg gccttcggct aa
//
LOCUS       DAOQAN010000518         1160 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_198568_length_1160_cov_2.711312, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000518 DAOQAN010000000
VERSION     DAOQAN010000518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_198568_length_1160_cov_2.711312"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="PKO10_07500"
     CDS             complement(<1..623)
                     /locus_tag="PKO10_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014473346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HNW67029.1"
                     /translation="MGLIDNIKEKAKKNLRTIVLPESEDERVLKATEIVLKDKTAKII
                     LIGNEETIKADAAKYGANISGATIIDPSKCTNLDKYVDELVELRKSKNLSKDEAKKLM
                     LTEPRFFGCMMVRLGDADGLVAGSNSPTSDVLRAAIQVIKTAPGINTVSSAFIMETAD
                     GKFGDNGLILFADCAVIPDPNAEQLADIASATAATAATAPAAPVAPVG"
     gene            complement(806..>1160)
                     /locus_tag="PKO10_07505"
     CDS             complement(806..>1160)
                     /locus_tag="PKO10_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HNW67030.1"
                     /translation="DLREVIKAANDGDKRSKIAITMLCDRIKKYLCSYAGLMHGVDAI
                     CFTAGIGENSDLIREKVCEGLEFMGIEIDKDKNSKREKGNREINTKNSKTKIFVIPTN
                     EELVIATDTYNLLKK"
BASE COUNT          372 a          208 c          147 g          433 t
ORIGIN      
        1 ccgaccggcg caaccggcgc agcaggagca gtagcagcag tagcagcagt agcactagca
       61 atatctgcta attgctcagc atttggatct ggaataacag cacaatctgc aaataatatt
      121 agaccattat ctccaaattt accatcagct gtttccataa taaatgcaga tgaaactgta
      181 tttataccag gggctgtttt tattacttgg atagcagctc ttaaaacatc agaagttgga
      241 gagtttgaac cagcaactaa tccatctgca tctcctagtc ttaccatcat acaaccaaaa
      301 aatctaggtt ctgtaagcat tagtttttta gcttcatctt ttgaaagatt tttacttttt
      361 ctaagttcaa ctaattcatc aacatattta tcaagatttg tgcattttga aggatcaata
      421 atagtcgccc cagaaatatt tgcaccatat tttgcagcat cagcttttat tgtttcttca
      481 tttccaatta ggataatttt tgcagtttta tcttttaaaa ctatttcagt tgcctttaaa
      541 actctctcat cttcagactc tggaagaaca atagttctta aatttttttt agctttttct
      601 ttgatattat caattaaacc catgatgtaa ccaatccttt cttttttcaa atataacgaa
      661 aagtataatg aaaaaaaata gcattaaaat agcaataaaa gcgatatttg agggctttgt
      721 aagtgtttaa aaaagtgaaa aataaaataa aaagagcact tttgagtaaa ttctacaagt
      781 ttgctctttt tatttttata aatttttact tttttagtaa attataagta tctgttgcta
      841 ttactaattc ttcatttgta ggaattacaa agatttttgt tttagagttt tttgtattta
      901 tctctctatt tcctttctct ctttttgagt ttttatcttt atcaatctca attcccataa
      961 actctaaacc ttcacaaact ttttctctaa ttaaatcaga gttttctcca attccagctg
     1021 taaaacaaat agcatcaaca ccatgcataa gtccagcata agagcaaaga tattttttga
     1081 ttctatcaca cagcatagta attgctattt tagatctttt atctccatca ttagcagctt
     1141 ttataacctc tcttaaatct
//
LOCUS       DAOQAN010000519         1160 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_198613_length_1160_cov_2.268778, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000519 DAOQAN010000000
VERSION     DAOQAN010000519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_198613_length_1160_cov_2.268778"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="PKO10_07510"
     CDS             complement(<1..510)
                     /locus_tag="PKO10_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012013141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdenumtransferase MoeA"
                     /protein_id="HNW67031.1"
                     /translation="MFEKLNYLDFNEAIKKSFELTNTTLSSEFVSINFAINRVVSEDI
                     LCVKNLPAFDNSAMDGFAFKASDAGQTLKIKRAIFAGDKDKSSDELLEKNECYKIMTG
                     AKVPKDADTIIAIENCINVTEDSVTIPVDIKKGSNLRLEAEELKISDILIKKGEKINS
                     SHIALLASQG"
     gene            complement(514..>1160)
                     /locus_tag="PKO10_07515"
     CDS             complement(514..>1160)
                     /locus_tag="PKO10_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HNW67032.1"
                     /translation="ILIFYVFLGSIDTFYKNSLENVKKLTDAKIISNASASFDKNSDV
                     IYFDSYKLTGFSTKSIILFNQNLFEEQAIFEKDGISLNNIFQSLKNQKFENSMKEIFK
                     EKLENSLKDNLYYFIDNKNSLEYTLHFALKGIKARELIRTLALDEILISNGEGCSLGL
                     SKPSRVIQAMGYDELTSRNAITLSFEKSYSISEIDKIVNLISKRYLQIKVLNKG"
BASE COUNT          398 a          176 c          108 g          478 t
ORIGIN      
        1 tccttgacta gcaagtaaag caatatgaga agagtttatt ttctcccctt ttttaattaa
       61 aatatcacta atttttaact cttcagcttc taatcttaag tttgaacctt ttttaatatc
      121 aacaggtata gttacactat cttctgtaac atttatacag ttttcaatag caataatagt
      181 atcagcatct tttggtacct tcgcacctgt cattattttg taacactcat ttttttcaag
      241 taactcatca ctacttttat ctttatcacc agcaaaaata gctcttttta tttttaaagt
      301 ttgtcctgca tcacttgctt taaaagcaaa tccatccata gcagagttat caaaagctgg
      361 aagatttttt acacataaaa tatcttcact tacaactcta tttatggcaa aattgataga
      421 aacaaattca ctacttaaag tagtatttgt caattcaaaa ctctttttaa ttgcttcatt
      481 aaaatctaaa tagtttaatt tttcaaacat tttttatcct ttatttaata cttttatctg
      541 taaatatctt tttgaaataa gattaacaat tttatctatt tcacttattg aatagctttt
      601 ttcaaaacta agtgttatag cgtttctgct agtaagttca tcataaccca tagcttgaat
      661 aactcttgaa ggttttgata aacctaaact acacccttct ccatttgaaa ttaaaatttc
      721 atctaaagct aaagttctta ttaactctct tgcttttata cctttaagag caaaatgaag
      781 tgtatattct aagctatttt tattgtcgat aaaatagtaa agattatctt ttaaactatt
      841 ttctaatttt tctttaaaaa tctctttcat actattttca aatttttgat ttttcaaaga
      901 ttgaaaaata ttatttagtg aaattccatc tttttcaaaa atagcttgtt cttcaaataa
      961 attttgatta aaaagtatta ttgattttgt tgaaaaacca gttaatttat atgaatcaaa
     1021 atatataaca tcactatttt tatcgaaaga ggcacttgca tttgagataa tttttgcatc
     1081 tgttagtttt tttacatttt ctaaactatt tttataaaaa gtatcaatac tgccaagaaa
     1141 aacataaaag attaaaattt
//
LOCUS       DAOQAN010000520         1160 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_198625_length_1160_cov_2.205430, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000520 DAOQAN010000000
VERSION     DAOQAN010000520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_198625_length_1160_cov_2.205430"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(90..725)
                     /locus_tag="PKO10_07520"
     CDS             complement(90..725)
                     /locus_tag="PKO10_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005871400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNW67033.1"
                     /translation="MSCSLNLKNISYVKEEKLLFKDINIDLGHKEKIAIVGANGVGKS
                     TLLKIMAGLIEPSSGEIEIFHNLIKNKRDFEKYRDDIGYLPQDVSSFFLCITVIEDIM
                     FSLQTLGIGKKEAYDKSKEILKNLNILHLENRVIYELSGGEQKIVALASILVKEPKIL
                     LLDEPTNALDEESENRILDILSKIDKSMIIVSHHKSFIEKIVSKIYNLSSL"
     gene            complement(722..>1160)
                     /locus_tag="PKO10_07525"
     CDS             complement(722..>1160)
                     /locus_tag="PKO10_07525"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HNW67034.1"
                     /translation="FNQAFNLFFKINLIILFNLLLFSSSNGFDIVKGFMMLRFPQKFI
                     SALYFTVKLIFELNLELKNIKLSLKARNFKPKTDIFTYKTYGNIFGILFLKMILKSNS
                     LKETLLLRGFKNQIFLNYDSKIYLYDIVLLFLIFFIFVLKVFL"
BASE COUNT          441 a          153 c          108 g          458 t
ORIGIN      
        1 tctatgtcca aatttccagc tactaatttt ggttatgtat taaaaaagta ggaaattttg
       61 tatttattta ctgaatttga gtaatttaat tataaagaac ttaaattata aatttttgag
      121 actatttttt caatgaaaga tttgtgatga gaaacaatta tcatagactt atctattttg
      181 cttaaaatat ctaaaattct attttcactc tcttcatcta aagcatttgt tggttcatct
      241 aagagcaaga tttttggctc ttttactaaa attgaagcaa gtgctacaat tttttgttca
      301 ccaccactaa gctcataaat aaccctgttt tctaaatgta atatattaag atttttcaat
      361 atctctttac ttttatcgta tgcttctttt tttccaattc ctaaagtttg caaagaaaac
      421 attatatctt caatcacagt aatacaaaga aaaaaactac tcacatcttg tggtaaatat
      481 cctatatcat ctctatattt ttcaaaatct cttttgtttt ttatcaaatt atgaaatatt
      541 tcaatttcac ctgatgaggg ttcaattaat cctgccataa ttttaagaag tgttgatttt
      601 ccaactccat ttgctccaac aatcgcaatt ttttctttat gtcctaaatc aatatttata
      661 tctttaaata atagtttctc ttcttttaca taagaaatat tttttaaatt tagtgagcaa
      721 ctcataaaaa aacctttaag acaaaaataa agaagattaa aaatagtaaa acaatatcat
      781 ataaatatat tttagaatca taatttaaaa atatttgatt tttaaaacct cgaagcaaaa
      841 gagtctcttt taaactattt gattttaaaa tcatcttcaa aaatagtatt ccaaatatat
      901 ttccatatgt tttataagta aatatatctg tttttggttt aaaatttcta gctttcaaag
      961 ataatttaat attctttaat tctaaattta gttcaaaaat aagttttact gtaaaatata
     1021 aagcggatat aaatttttgt ggaaatctta gcatcataaa tccctttaca atatcaaaac
     1081 catttgaaga agagaacaat aaaagattaa ataaaataat caaatttatc ttaaaaaata
     1141 gattaaaagc ttgattaaaa
//
LOCUS       DAOQAN010000521         1159 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_198894_length_1159_cov_2.145833, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000521 DAOQAN010000000
VERSION     DAOQAN010000521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_198894_length_1159_cov_2.145833"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>604
                     /gene="argH"
                     /locus_tag="PKO10_07530"
     CDS             <1..>604
                     /gene="argH"
                     /locus_tag="PKO10_07530"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HNW67035.1"
                     /translation="KTKSIQNQIKELISTFVEIASKHTTTIMPGMTHLQHAQPINFGF
                     HMMAYANMFKRDFERFESSYIRNNYSPLGSAAMAGTPHNIDRFKTAELLGFLAPTQNA
                     MDSVSNRDFALELLFNISTTMMHVSRISEELILWSSYEFRFIQMSDEYATTSSIMPQK
                     KNPDVPELLRGKTGRVYGNLISLLTIMKSLPLAYNKDTQE"
     assembly_gap    605..614
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <615..1038
                     /locus_tag="PKO10_07535"
     CDS             <615..1038
                     /locus_tag="PKO10_07535"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468335.1"
                     /note="catalyzes the formation of arginine from
                     (N-L-arginino)succinate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HNW67036.1"
                     /translation="DKEGVFDSVETIEISLNILNEVIKTMIVKEENMKSACKIGHLTA
                     TDLADYLVAKQNMPFRTAYYITKEVVQKANSLNKDISELNISEIRSSTKELENIDEEI
                     VSYLSLENSMNSRNSFGGTSTKQTEIQIAYFKDWLSNS"
BASE COUNT          440 a          150 c          180 g          379 t
ORIGIN      
        1 aaaaaacaaa atctattcaa aatcaaataa aagagttaat atctactttt gttgaaattg
       61 cttcaaaaca tacaacaaca ataatgccag gaatgacaca tttgcaacat gcacaaccaa
      121 ttaattttgg ttttcatatg atggcatatg caaatatgtt taaaagagat tttgaaagat
      181 ttgaaagctc atatataaga aataactact cacctcttgg aagtgctgca atggcaggaa
      241 ctccacacaa tattgataga tttaaaacag cagaactttt aggatttttg gctccaacac
      301 aaaatgctat ggatagtgtt tctaatagag attttgcttt ggaattgcta tttaatatct
      361 ctacaacaat gatgcatgtt agtcgtattt cagaagagct tattttatgg agttcttatg
      421 agtttagatt tattcaaatg agtgatgagt atgcaacaac aagttctatt atgccacaaa
      481 agaaaaaccc agatgtacca gaacttttaa gaggtaaaac tggtcgtgta tatggaaatt
      541 taatttcact tttaacaatt atgaaatcac ttccactagc ttataacaaa gatacacaag
      601 aggannnnnn nnnnggataa agaaggagtt tttgattctg ttgaaactat tgaaatctcg
      661 cttaatattt taaatgaagt tataaaaact atgattgtaa aagaagaaaa tatgaagagt
      721 gcttgtaaaa taggacattt gacagctact gatttggctg attatttggt tgcaaaacaa
      781 aatatgccat ttagaacagc ttactatatt acaaaagagg ttgttcaaaa agcaaatagt
      841 ttaaataaag atataagtga gttaaatata tctgagatta gaagttcaac aaaagagctt
      901 gaaaatatag atgaagagat agtttcatat ttgagtttag aaaactctat gaatagtaga
      961 aactcatttg gtggaacatc tacaaaacag actgaaattc aaattgctta ttttaaagat
     1021 tggttatcaa attcttgata ataaaaatgt agttaatact acatttttat tagttatatc
     1081 tctttaattt tagcaatctc atctctaagt cgtatagcct cttcaaagtt taaatcactt
     1141 gcagcttttt tcatctgtt
//
LOCUS       DAOQAN010000522         1158 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_199125_length_1158_cov_2.549411, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000522 DAOQAN010000000
VERSION     DAOQAN010000522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_199125_length_1158_cov_2.549411"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..>1158
                     /locus_tag="PKO10_07540"
     CDS             2..>1158
                     /locus_tag="PKO10_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNW67037.1"
                     /translation="MGGSLISILNKDKDLVLFEKDKNLGGTASTFKRFGNYFNSGATT
                     FVGYEENHILKDIFDIANFTPDLIESSYAYRTIIDGKIIDRKCDFEEFLDSLNTVFYH
                     KNNRYFWQTLKDIDERFWRLKDIYFAKYSLNSYLKTLKTVEILFKEYKFLMFKSAKSF
                     IKEVLGDISKEYEDFIDAQLQITLQSSSKDIPLLSFAIALSYPFHKIFYANGGMGKLF
                     DDMLKDIDVRKSEEIKYIKKERDFYRLISTKNEYLSSKVVLNAPVFECSEIFLDEDIK
                     NYYKKFEFYDQSAFVIYLKIDSQKEFLNHYQIILKDTIPNSVSKSFFVSFSHKDDEKL
                     SKNGYSVTISCHTKALFWSNLSKDEYETKKELTKNFILDEFANMPALKDVEA"
BASE COUNT          453 a          116 c          178 g          411 t
ORIGIN      
        1 aataggtgga agcttaatct caattttaaa taaagataaa gatttagttc tatttgaaaa
       61 agataaaaac ctaggtggaa ctgcatctac atttaaaaga tttggaaact attttaatag
      121 tggggctaca acttttgttg gttatgaaga gaatcacatt ttaaaagata tatttgatat
      181 tgcaaatttt acccctgatt taatagagag ttcttatgca tatagaacta ttatagatgg
      241 aaaaattatt gatagaaaat gtgattttga agagttctta gatagtttaa acacggtttt
      301 ttatcataaa aacaatagat atttttggca aactttaaaa gatattgatg agagattttg
      361 gagattaaaa gatatatatt ttgcaaaata tagtttaaac tcttacttaa aaactctaaa
      421 aacagttgag atacttttta aagagtataa atttttgatg tttaaaagtg ccaaaagttt
      481 tataaaagag gttttaggtg atatttcaaa agagtatgaa gattttatag atgctcaact
      541 tcaaataact cttcaatcaa gttcaaaaga tattccactt ctatcttttg caattgcttt
      601 atcataccct tttcataaga ttttttatgc aaatggtgga atgggaaaac tgtttgatga
      661 tatgctaaaa gatatagatg taagaaaaag tgaagagata aaatatataa aaaaagagag
      721 agatttttat agattaatct ctacaaaaaa tgagtatcta tcttcaaaag ttgttttaaa
      781 tgctcctgtt tttgagtgta gtgagatttt tttagatgaa gatattaaaa attactataa
      841 aaaatttgag ttttatgatc aaagtgcttt tgtaatttat ttaaagattg attcacaaaa
      901 agagttttta aatcactatc aaattatttt aaaagatacc attccaaata gtgtatcaaa
      961 aagttttttt gtatctttta gccacaaaga tgatgaaaaa ctatcaaaaa atggctactc
     1021 tgttacaatt tcatgtcata caaaggctct attttggagt aatctttcaa aagatgagta
     1081 tgagacaaaa aaagagctta caaaaaactt tatattagat gagttcgcta atatgccagc
     1141 tcttaaagat gttgaagc
//
LOCUS       DAOQAN010000523         1157 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_199452_length_1157_cov_2.072595, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000523 DAOQAN010000000
VERSION     DAOQAN010000523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_199452_length_1157_cov_2.072595"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            173..685
                     /locus_tag="PKO10_07545"
     CDS             173..685
                     /locus_tag="PKO10_07545"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011373089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNW67038.1"
                     /translation="MSTNRFGKELKEYNYTKEELAKFNYAKITTKNGEILIKLFNQET
                     PNTVANFVTLANDGFYDGLNFHRVIAGFMAQGGCPQKSGMGGPEWAIKCEVNAPKQNH
                     KRGSLSMAHAGRDTGGSQFFICFVPCPHLDGNHTVFGEIEVTQNSSFKTLDAIKQGDE
                     IIKIEILESI"
     gene            complement(727..>1157)
                     /locus_tag="PKO10_07550"
     CDS             complement(727..>1157)
                     /locus_tag="PKO10_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methylamine utilization protein MauG"
                     /protein_id="HNW67039.1"
                     /translation="GETFTNYEFHNIGVPVNNELREKNGINEKDLGLAANPNIKNKDE
                     NLGKYKTPTLRNVAVTAPYMHNGVFKDLRTVVEFYDKYNNKERTLNPETKKPWDEPEH
                     KDTISLKELKANAQTDRKIDALVAFMKLLTDKRYEHLIKD"
BASE COUNT          405 a          164 c          178 g          410 t
ORIGIN      
        1 actcatatac tattggagtt acagtaataa acccaaaccc aataagacca cactattatg
       61 cagcacaatc agcagttcca gtttttaaag agattattca aaatcttata aaactaaact
      121 acttatcccc taaagaggat ataaatttag aaaattaaaa aaaaggatat ttatgagtac
      181 aaatagattt ggaaaagagt taaaagagta taattataca aaagaggagc ttgcaaaatt
      241 taattatgca aaaatcacta caaaaaatgg agagatttta ataaaacttt ttaaccaaga
      301 gacaccaaat acagttgcaa attttgtaac tttggcaaat gatgggtttt atgatggatt
      361 aaattttcat agagtaatag ctggttttat ggcacaaggt ggatgtccac aaaaatcagg
      421 tatgggtggt ccagagtggg ctataaaatg tgaagtgaat gctcctaaac aaaatcataa
      481 aagaggaagc ctttcaatgg cacatgcagg aagagataca ggtggtagtc aatttttcat
      541 atgttttgta ccttgtccac atttagatgg taaccacact gtttttggag aaattgaagt
      601 aactcaaaat agtagtttta aaactttaga tgctataaaa caaggtgatg agataatcaa
      661 aattgagatt ttagaatcta tataatattg acaatttaaa aaggtgaggg ctaactcacc
      721 tttttttcaa tcttttatta gatgttcata tcttttatct gttaaaagtt tcataaatgc
      781 tactaaagca tctatttttc tatcagtttg agcatttgct tttagctctt ttaatgaaat
      841 tgtatcttta tgttcaggct catcccaagg tttttttgtt tcaggattta gagttctctc
      901 tttgttattg tatttatcat aaaattcaac aaccgttctt aaatctttaa atacaccatt
      961 atgcatatac ggagcagtaa ctgcaacatt tcttagagta ggagttttgt attttcctaa
     1021 attttcatct ttgtttttaa tatttgggtt tgctgctaaa cctaaatctt tttcatttat
     1081 accatttttc tctcttaatt cattatttac tggaactcca atattatgaa actcatagtt
     1141 tgtaaatgtt tctccag
//
LOCUS       DAOQAN010000524         1157 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_199484_length_1157_cov_1.860254, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000524 DAOQAN010000000
VERSION     DAOQAN010000524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_199484_length_1157_cov_1.860254"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            18..1154
                     /gene="trmA"
                     /locus_tag="PKO10_07555"
     CDS             18..1154
                     /gene="trmA"
                     /locus_tag="PKO10_07555"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014469455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - S-adenosylmethionine-dependent
                     tRNA (m5U54) methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HNW67040.1"
                     /translation="MTCKYFGLCASCTLYDKNYDEQLNYKIQREKNRFSNLTTCDFDI
                     IKSTPKNFRNRAEFRVWWEKDEQNNKDILTYAMNDFKKEILKIDSCEIVNEDIKNLMP
                     KLLTELEKSMILSFRLFAIEFLVSSTSDMLVTLIYHKKLEDEWINLAREIEQKLNIKI
                     IGRSRKQKIVLSSDYINESLNINNQEFKFAYEENGFTQPNTSVNIKMIEWVLNNIEKS
                     NKDLCELYCGGGNFTIPLSKKFSKVLATEISKTSIKSALRNCALNNIENIDFIRMSSE
                     EFVEALNEVRVFNRLKNIDLKSYDFDTIFMDPPRSGLDDTTRTLAKKFENIIYISCNP
                     ETLHRDLEELLKTHKIVRFALFDQFAYSEHIECGVILEKNANTI"
BASE COUNT          472 a          121 c          184 g          380 t
ORIGIN      
        1 aaaaagggat atattttatg acatgcaaat attttgggct ttgtgcttct tgtacacttt
       61 atgataaaaa ttatgatgaa caactaaact acaaaatcca aagagaaaaa aatagatttt
      121 caaatcttac gacttgcgat tttgatatta taaaaagtac tccaaaaaac tttagaaata
      181 gagctgaatt tagagtttgg tgggaaaaag atgagcagaa caacaaagat attttaactt
      241 acgccatgaa tgatttcaag aaagagattt taaaaataga ttcatgtgag atagtaaacg
      301 aagatataaa aaatcttatg ccaaaactat taactgagct tgaaaaatct atgattttgt
      361 cttttaggct ttttgctatt gagtttttag ttagttcaac ttctgatatg cttgtaactt
      421 tgatttatca caaaaaactt gaagatgagt ggataaatct tgcacgtgaa attgagcaaa
      481 aattaaatat aaaaataatt ggaagaagca gaaaacaaaa aattgtttta agtagcgatt
      541 atataaatga gagcttaaat ataaacaatc aagagttcaa atttgcctat gaagagaatg
      601 gttttactca accaaatact tctgtaaata taaaaatgat tgagtgggtt ttaaataata
      661 tagaaaagtc aaataaagat ttatgtgagc tttattgtgg tggtggaaat tttacaattc
      721 ctctttcaaa aaaattcagt aaagtattag caacagagat ttcaaaaaca tctataaaat
      781 cagctttaag aaattgtgct ttaaataata ttgaaaatat agattttata agaatgagct
      841 cagaagagtt tgttgaagct ttaaatgaag ttagagtttt taatagacta aaaaatatag
      901 atttaaaatc ttatgatttt gatactattt ttatggatcc accaagaagt ggacttgatg
      961 atacaacaag aactttagca aaaaaatttg aaaatattat ttatatctct tgcaatcctg
     1021 aaactttaca tagagactta gaagagcttt taaaaactca taaaatagta agatttgccc
     1081 tatttgacca atttgcatat agtgagcata tagagtgtgg tgtgattttg gaaaaaaatg
     1141 caaatacgat ataattt
//
LOCUS       DAOQAN010000525         1156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_199597_length_1156_cov_3.524977, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000525 DAOQAN010000000
VERSION     DAOQAN010000525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_199597_length_1156_cov_3.524977"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="PKO10_07560"
     CDS             complement(<1..448)
                     /locus_tag="PKO10_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW67041.1"
                     /translation="MVDNSLQNNNYGLGLYSNNSLQNSTQTAGKTTSGEKELEQAKDN
                     KVKDNEAVSLSKNVEKAVDTKSISKSNEFENFQNKINSFTTDNKTIKDIQEDMKLLSS
                     ADGEVDTKKVNDRINQNIDKLQNLSQDEKDKLKENLNGVDNIVNNNI"
     gene            584..>1156
                     /locus_tag="PKO10_07565"
     CDS             584..>1156
                     /locus_tag="PKO10_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase dimerization
                     domain-containing protein"
                     /protein_id="HNW67042.1"
                     /translation="MKLTLIGNGIMAQSLAIGLVKEHEVEMIGRELNKLKLIKEKIPE
                     LQIKELSDKEDISGKNIIFCVKPYALESVSIRLVGTANSLISILAGTKLDYLRKQISA
                     KHYARTMPNIAASVQNSMTTATGDIEIKDLTLEAFGSIGEAIWVNNENQLDIASAVTA
                     SGPAFLALVAEAIADGAVKVGLERHLAQHLV"
BASE COUNT          382 a          151 c          167 g          456 t
ORIGIN      
        1 ttatattgtt atttacaata ttatcaacac catttagatt ctcttttaac ttatcttttt
       61 catcttgtga caagttttgt agcttatcga tattttgatt tattctatca tttacttttt
      121 ttgtatctac ttcaccatct gctgaagata aaagtttcat atcttcttgt atatctttta
      181 ttgttttatt atctgttgta aaagagttta ttttattttg aaaattttca aactcatttg
      241 attttgaaat actttttgta tctactgctt tttcaacatt tttagataaa gataccgctt
      301 cattatcttt taccttatta tcttttgctt gttctaactc tttttcccca gaagttgttt
      361 ttccagcagt ttgcgtagag ttttgtaaac tgttattgct ataaagtccc agtccgtaat
      421 tgttattttg taaactatta tctaccattt tgaactcctt gttatctgtt ttgattttac
      481 ttgataaaaa cttaagattt catatatttt ttatttttaa aattaaacat agatttatat
      541 cttaaatttg ttataatctt aaaaaataat tttaaggcag agtatgaaat taacacttat
      601 tggaaatgga attatggctc aatctttagc tattgggctt gtaaaagagc atgaagttga
      661 gatgatagga agagaactaa ataaattaaa actaataaaa gagaaaatcc ctgaactaca
      721 aataaaagag ttaagtgaca aagaagatat tagtggaaaa aatataattt tttgtgtaaa
      781 accctatgca ttagagagtg tttcaataag actagtaggt actgcaaatt cattaatatc
      841 tattttagct ggtacaaaat tagattattt aagaaaacaa attagtgcaa aacactatgc
      901 aagaactatg ccaaatattg ctgcaagtgt acaaaactct atgacaacag ctacaggaga
      961 tattgagata aaagatttaa ctcttgaagc ttttggatct attggtgaag ctatttgggt
     1021 aaataatgaa aatcaactag atattgctag tgcagttaca gcttctggac ctgctttttt
     1081 agctttagtt gctgaagcta tagcagatgg agctgttaaa gttggacttg aaagacattt
     1141 agctcaacac ttagtt
//
LOCUS       DAOQAN010000526         1156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_199784_length_1156_cov_1.622162, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000526 DAOQAN010000000
VERSION     DAOQAN010000526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_199784_length_1156_cov_1.622162"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..802
                     /locus_tag="PKO10_07570"
     CDS             <1..802
                     /locus_tag="PKO10_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HNW67043.1"
                     /translation="ELIIAKYTREAGVRNLRRVFSKIFRKVVKKILEDETIAKVTIGI
                     KDLKEYLDNPIFEIEPADKVDVVGVSNGLAWTAVGGDILKIEAIKLKGKGDLKVTGNL
                     GDVMKESSIISYSVVKHLIDNKILKIDEKDIPKTFKEKDEKELVDCSEIYKRYDIHLH
                     IPEGATPKDGPSAGITMALALASVLSNRKIKADVAMTGELTLSGKVLPIGGLKEKLIA
                     AYKAKIKKVLIPKKNYERDLDDIPQEVKESLEIKVVEKIEDVLKEALI"
     gene            802..>1156
                     /locus_tag="PKO10_07575"
     CDS             802..>1156
                     /locus_tag="PKO10_07575"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNW67044.1"
                     /translation="MSFVSKKDLSATNVIIFITLVAYIIQINIQNGSLLMGLNLYFLV
                     GGFYWQPLTSIFSHGGIAHLGMNMFVLWQFGNLIEKARGKKRFIILYLFTGILTSLLS
                     FLYIFYIDIQVNLVGA"
BASE COUNT          459 a          110 c          209 g          378 t
ORIGIN      
        1 tgagctaata attgcaaaat atacaagaga agcaggggtt agaaatttaa gaagagtctt
       61 ctcaaagata tttagaaaag ttgtaaaaaa gatattagaa gatgagacta ttgcaaaagt
      121 aacaattgga ataaaagatt taaaagagta tttagataat ccaatttttg agatagaacc
      181 agcagataaa gtagatgttg ttggagtttc aaatggtttg gcttggacag ctgttggtgg
      241 ggatatatta aaaatagagg ctataaaact aaaaggaaaa ggtgatttaa aagtaactgg
      301 taatttaggc gatgttatga aagagtcatc aattatctct tattcagttg taaaacactt
      361 aatagataat aaaattctaa aaatagatga aaaagatatt ccaaaaactt ttaaagaaaa
      421 agatgaaaaa gagctagttg attgtagtga aatttataaa agatatgata ttcatctaca
      481 tattcctgaa ggggcaactc caaaagatgg accaagtgca ggaataacaa tggctttagc
      541 tttggcttca gttttaagta atagaaaaat aaaagcagat gtagcaatga caggtgagct
      601 tactttaagt ggaaaagttc ttccaattgg tggtttaaaa gagaaattaa tagctgctta
      661 taaagcaaaa attaaaaaag ttctaattcc taaaaagaat tatgaaagag atttagatga
      721 tattccacaa gaggtaaaag agtctttaga aataaaagta gttgaaaaaa tagaagatgt
      781 tttaaaagag gctttaattt aatgagtttt gtatctaaaa aagatttgag tgctacaaat
      841 gtgataattt ttatcactct tgtagcttat attattcaaa taaatataca aaatggctca
      901 cttcttatgg gattaaacct atatttttta gttggtggat tttattggca accacttaca
      961 tcaatatttt ctcatggtgg aattgctcac cttggaatga atatgtttgt tttatggcaa
     1021 tttggaaatc ttatagaaaa agctagaggt aaaaaaagat ttataatact atatttattt
     1081 acaggtattt taacttctct tttatcgttt ttatatattt tttatattga tattcaagtc
     1141 aatttagtag gagcaa
//
LOCUS       DAOQAN010000527         1153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_200500_length_1153_cov_1.979053, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000527 DAOQAN010000000
VERSION     DAOQAN010000527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_200500_length_1153_cov_1.979053"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(143..829)
                     /locus_tag="PKO10_07580"
     CDS             complement(143..829)
                     /locus_tag="PKO10_07580"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNW67045.1"
                     /translation="MRENLKNFTLLYAEDDKAIQKEMLEYFSSYFKEVFTANDGKEAL
                     EIYKQNRPDVMILDIYMPHLTGIELTKILRENDYKTKIVLITAHSNDTLLLEAINIDV
                     NYYLIKPATLKKVKDMLDKISIDLLRSSEKIVRFDENIYFNQTSKKLYNKNIELKLSK
                     KELSLLELFIINRNKDITIEDIMSHCWSDIFIEVSLDSVKSLVSNLRKKLPKDTILNV
                     YGVGYILKNS"
     gene            complement(826..>1153)
                     /locus_tag="PKO10_07585"
     CDS             complement(826..>1153)
                     /locus_tag="PKO10_07585"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNW67046.1"
                     /translation="QVIISILSNSIDIFKARDIEKKYINIDIYEDDIHYFISIKDSAG
                     GIKEDIIDKIFEPYFSTKHKSGGTGMGLYISKLIIQSSFKGDIKVKAVDDGVKFILEI
                     PKGKEF"
BASE COUNT          394 a          154 c          112 g          493 t
ORIGIN      
        1 caatctcaat gttttgatag ttatgattta tataattaat taacatgtaa tatcctataa
       61 attgtttcct agaagtgaat tataaaaaaa gagtgtgatg aagtgtgaag tttttaataa
      121 aaaaatagtt aaatttaaga ttttaactat tttttaaaat gtaaccaaca ccataaacat
      181 ttaaaatagt atcttttgga agtttttttc ttaagttact aaccaaagat tttacactat
      241 caagtgatac ttcaataaaa atatcactcc agcaatgaga cataatatct tcaatagtta
      301 tatctttatt tctatttatt ataaaaagtt ctagcaaaga gagctctttt ttactaagtt
      361 ttagctcaat atttttatta tagagttttt tactagtttg attaaaatat atattttcat
      421 caaatcttac tattttttca gagcttctta ataaatcaat agagatttta tcaagcatat
      481 cttttacttt tttcaaagtt gctggtttta ttaaatagta atttacatct atgtttatgg
      541 cttccaaaag taaagtgtcg tttgagtgtg cagttattaa aactattttt gttttatagt
      601 cattttctct taaaattttt gttaattcaa ttcctgttaa atgaggcata taaatatcaa
      661 gtatcataac atctggtcta ttttgtttat atatttctaa agcttcttta ccatcatttg
      721 cagtaaaaac ctctttaaaa tagctagaaa aatactccaa catctctttt tgaatagctt
      781 tatcatcttc agcataaagc aatgtaaaat tttttaaatt ttctctcaaa actcttttcc
      841 ttttggtatt tctaatataa attttactcc atcatctaca gcttttactt ttatatcgcc
      901 tttaaaacta ctttgtataa taagtttaga gatatataag cccattccag tacctccact
      961 tttatgtttt gttgaaaaat atggttcaaa tattttatct ataatatcct cttttatacc
     1021 accagcacta tcttttatag atataaaata gtgaatatca tcttcgtaaa tatctatatt
     1081 tatatatttt ttttctatat ctcttgcttt aaatatatct attgaatttg ataatataga
     1141 tattataact tgc
//
LOCUS       DAOQAN010000528         1146 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_202384_length_1146_cov_2.384051, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000528 DAOQAN010000000
VERSION     DAOQAN010000528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_202384_length_1146_cov_2.384051"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..981)
                     /gene="prpF"
                     /locus_tag="PKO10_07590"
     CDS             complement(<1..981)
                     /gene="prpF"
                     /locus_tag="PKO10_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014473135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylaconitate cis-trans isomerase PrpF"
                     /protein_id="HNW67047.1"
                     /translation="MSSYRPQIRVKATYMRGGTSKGTFFNIADLPKEAQEDKVKRDKL
                     LQRIVGSPDIYKQQMDGMGGATSSTSKAILVGKSTVPNHDVDYYFGQVAIDKDFMDWS
                     GNCGNLSSAVGPFAIHEGLVDNVPQNGVCCVRIWQANIKKTILCYVTMVDGQVKEMGD
                     YYIDGVAFPAQEILLEFAEPVDPSEELFPTGNLVDDLEVPGIGTFKATMITAGIPTIF
                     LNAADIGYKGTELQADINSDAQALARFEKIRSYGALKMGLISDLSEAETRQHTPKIAF
                     VAPKSDFTTSSGKEVKASEIDLHVRALSMQKLHHAMMGTASVAIGVAACIE"
BASE COUNT          353 a          223 c          147 g          423 t
ORIGIN      
        1 ttcaatacaa gctgcaactc caatagctac tgaagctgtt cccatcatag catgatgaag
       61 tttttgcata gataaagcac gtacgtgtaa atcaatttcg ctagctttta cctctttacc
      121 acttgaagtt gtaaaatcag attttggtgc aacaaaagca atttttggag tgtgttgtct
      181 agtttctgct tcacttaaat cacttatgag acccattttt aaagcaccat aacttcttat
      241 tttttcaaat cttgctaagg cttgggcgtc actattaata tcagcttgaa gttctgttcc
      301 tttatatcca atatcagcag catttaaaaa tattgtagga attcctgctg ttatcatagt
      361 tgctttaaat gttccaattc caggaacttc taaatcatct actaaatttc ccgtaggaaa
      421 taattcttca cttggatcaa ctggttctgc aaattctaat aaaatctctt gggcagggaa
      481 agcaacacca tcaatatagt aatcacccat ctcttttact tgtccatcaa ccattgttac
      541 ataacaaaga attgtttttt tgatatttgc ttgccaaatt cttacacaac aaacaccatt
      601 ttgtggtaca ttatcaacta aaccttcatg aatagcaaaa ggtccaacag ctgaacttaa
      661 atttccacaa tttccactcc agtccataaa atctttgtca attgcaactt gtccaaaata
      721 gtaatcaaca tcgtggtttg gaacagttga ttttccaaca agtattgctt tagaagtgct
      781 tgaagttgct cctcccatac catccatttg ttgtttataa atatcaggac ttcctactat
      841 tctttgaaga agtttatctc tttttacttt atcttcttgt gcttcttttg gtaagtctgc
      901 tatattaaaa aatgtacctt ttgaagttcc acctctcata tatgttgctt taactcttat
      961 ttgtggtcta taactactca tattttctcc taaaatttaa gcttttaaaa ctttttaaaa
     1021 tgtataaatt atatcacttt aaaaaatttt aaaatttagg aatagggcaa agccccaatc
     1081 ctaattattt attttcagca ataacatctt ttgcgaattt ttgtaaaatt ccaccagctt
     1141 tgtaaa
//
LOCUS       DAOQAN010000529         1144 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_202851_length_1144_cov_3.374656, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000529 DAOQAN010000000
VERSION     DAOQAN010000529.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_202851_length_1144_cov_3.374656"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="PKO10_07595"
     CDS             <1..529
                     /locus_tag="PKO10_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008335270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNW67048.1"
                     /translation="FTTPPPRFKEIINNENKNLEPEYIHPSMDKKRRELPFSLEGRTH
                     YDEGYLTPEQVNFIFKFLPVDITYVDENDRVVFYNRGDDRVFPRSAGIIGREVKFCHP
                     PKSVDQVLKILEEFKAGRQDIADFWINFKGKFVHIRYFAVRDEQKNYKGVIEMSQDVT
                     DIRALQGEKRLLDWE"
     gene            complement(690..>1144)
                     /locus_tag="PKO10_07600"
     CDS             complement(690..>1144)
                     /locus_tag="PKO10_07600"
                     /inference="COORDINATES: protein motif:HMM:NF039206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5718 family protein"
                     /protein_id="HNW67049.1"
                     /translation="CDFVYENEKVIDIVPKYFSAFNDCSLRFQDGSKLSTKKNWGEKT
                     KGISQDIIPIDDFSEKGILGKYHISSFIKRDGIVYDYGTTSSVKSYSYFFEKLKDWMI
                     DILNCQEDCGPLEELGQFLKYAHNSKGIVIAAGATAYTDFGKKNFLKR"
BASE COUNT          407 a          170 c          164 g          403 t
ORIGIN      
        1 gtttacaact cctcccccta gatttaaaga gattataaat aatgaaaata aaaatttaga
       61 accagagtat attcatccaa gtatggataa gaaaagaaga gaattaccat tctctcttga
      121 gggacgaact cattatgatg aaggttattt aacacctgaa caagtaaact ttatatttaa
      181 gttcttacct gtagatatta cttatgtaga tgaaaatgat agagttgttt tttataatcg
      241 tggagatgat agagttttcc caagaagtgc tggaataatt ggaagagagg ttaaattttg
      301 ccaccctcca aagagtgttg atcaggttct aaaaattctt gaagagttta aagcaggacg
      361 tcaagatatt gctgattttt ggattaattt taaaggaaag tttgtacata taagatattt
      421 tgctgtaaga gatgagcaaa aaaattataa aggtgtaatt gaaatgtcgc aagatgttac
      481 agatataaga gcattacaag gagagaaacg acttttagat tgggagtagc agattttaaa
      541 atctgctact tattatttgt aataaaatct attttactat ttggtgtagc tttgaacatt
      601 gtcctaaata tatatccatt ccacacatat cattcataat atcttgaaaa ctatgagcat
      661 gtgcattata aacatataca aaaatctcat caccttttca agaagttttt cttaccaaaa
      721 tctgtatatg ctgttgcacc tgcagctatt acaatacctt ttgaattgtg tgcatatttt
      781 aaaaactgtc ctaactcttc aagaggtcca caatcttctt ggcaatttaa gatatcaatc
      841 atccaatctt ttaacttttc aaaaaaatag ctatatgatt taactgaact agttgttcca
      901 taatcatata ctatcccatc tctttttata aaagaagaga tatgatattt tcctaaaatt
      961 cctttttcac taaaatcatc tataggaatt atatcttgag aaataccttt agttttttct
     1021 ccccaatttt ttttggtgct aagtttgctt ccatcttgaa atcttaatga acaatcatta
     1081 aaagcactaa aatattttgg aacaatatct attacctttt cattttcata tacgaaatca
     1141 caaa
//
LOCUS       DAOQAN010000530         1141 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_203856_length_1141_cov_2.118785, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000530 DAOQAN010000000
VERSION     DAOQAN010000530.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_203856_length_1141_cov_2.118785"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(229..>1141)
                     /gene="groEL"
                     /locus_tag="PKO10_07605"
     CDS             complement(229..>1141)
                     /gene="groEL"
                     /locus_tag="PKO10_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002344612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HNW67050.1"
                     /translation="GRPLVIISEDVEGEALATLVVNTMRKVLRAVAVKAPGFGDRRKA
                     MLEDIAVLTGGTVVSEEMGMKLETTDLSALGTASKIVIDKDNTTIVDGSGSKDAVLAR
                     VNQIKAEIANTTSDYDREKLQERLAKLSGGVAVIKVGAATETEMKEKKDRVDDALSAT
                     RAAVEEGIVIGGGAALIRAAAKVKLDLCGDEQIGAAIVLRAIKAPLKQIALNAGYDAG
                     VVANEVEKSSNENLGFNAATGEYVDMFEAGIVDPAKVERVAMQNAVSVASLLLTTEAT
                     VTDIKEDRPSMPDMSGMGGMGGMPGMM"
BASE COUNT          352 a          254 c          143 g          392 t
ORIGIN      
        1 aattgatgtc ttaattttgt tattttcatg aataaaattt tatcaaataa attattataa
       61 agttattaaa aaatttataa agtttacatt cctacaacta atttggacaa atctagacaa
      121 acttagtcat agctagacaa ctgtggttaa gaaaaagtta ggaaaagtgg acatcgtacc
      181 ccaaagaggt acgataaata gacatagtta gacaaatctg gacaattgtt acatcattcc
      241 tggcattccg cccataccac ccattccgct catatcaggc atactaggtc tatcttcttt
      301 aatatctgta acagttgctt ctgttgttaa aagtagtgat gctactgaaa cagcattttg
      361 cattgcaact ctttcaactt ttgcaggatc tacaattcca gcctcaaaca tatctacata
      421 ctcaccagtt gcagcattaa atcctaagtt ttcatttgaa gatttttcaa cctcatttgc
      481 aacaactcca gcatcatatc cagcatttaa agcaatctgt tttaatggtg ctttaatagc
      541 tcttaaaaca atagcagctc cgatttgctc atcaccacaa agatcaagtt ttactttagc
      601 agcagctcta attaatgcag ctcctccacc aattacaatt ccctcttcaa cagcagctct
      661 tgttgcactt aaagcatcat caactctatc ttttttctct ttcatttcag tctctgttgc
      721 agctccaact ttaattacag caactccacc acttaatttt gcaagtctct cttgtaattt
      781 ttctctatca taatcgcttg ttgtatttgc aatttctgct ttaatttggt ttactcttgc
      841 aagaactgca tctttacttc cacttccatc aacaatagtt gtattatctt tatcaattac
      901 aatttttgaa gcagttccaa gtgcgcttaa atctgtagtt tcaagtttca ttcccatctc
      961 ttcagataca acagttccac cagttaatac agcaatatct tcaagcatag cttttcttct
     1021 gtcaccaaat cctggagctt taacagcaac agctcttaaa acttttctca tagtgttaac
     1081 aacaagagtt gcaagagctt caccttcaac atcttcagaa atgatcacaa gaggacgtcc
     1141 t
//
LOCUS       DAOQAN010000531         1135 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_205380_length_1135_cov_3.398148, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000531 DAOQAN010000000
VERSION     DAOQAN010000531.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_205380_length_1135_cov_3.398148"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..840)
                     /locus_tag="PKO10_07610"
     CDS             complement(<1..840)
                     /locus_tag="PKO10_07610"
                     /inference="COORDINATES: protein motif:HMM:NF013698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNW67051.1"
                     /translation="MYYVGVDIAKEKHYVCILDDAKELACKPFWIYSDILGLRELLKR
                     LAELSLDMNDFIIGIESTGAFSENFYSYITDADYKVILLNSYQTSKYRDFSTLKKIKN
                     DSIDAYVIAELLASGKYKASYISNEDYHNLKVLNRLKKSLDDKIKTIKREISTVVATV
                     NPEIEKVFPNIFTKSALAIIKSYPTAMDISKATPEKLTKIFRHIKGNSFNLEKAKYLI
                     ELAKSSIYTGRANESRALMIKTNIKILELYIQEKDEVEKQILDLIDNQLDDFPARLPD
                     KRVF"
BASE COUNT          394 a          168 c          152 g          421 t
ORIGIN      
        1 gaaaacccgt ttatctggaa gacgcgcggg gaaatcatca agttgattgt caattaaatc
       61 tagaatttgt ttttcaactt catctttttc ttgaatataa agctctaata tcttaatatt
      121 agtcttgatc attaaagctc tagattcatt tgcacgacct gtataaatag aagacttagc
      181 aagttctata agatattttg ctttttcaag attaaagcta ttacctttga tatgtcgaaa
      241 aatcttagtt agcttttctg gcgtagcttt tgaaatatcc attgcggttg ggtaagattt
      301 aatgatcgct aaagcagatt ttgtaaagat atttggaaat actttttcaa tctctggatt
      361 aacagtagca acaacagttg atatctctct tttaatagtt tttattttat catctaatga
      421 tttttttaat cgatttaaaa cttttaaatt gtgataatct tcattgctaa tataactagc
      481 tttgtattta ccactagcaa gtaattcagc aataacataa gcatcaattg aatcattttt
      541 aatcttttta agagtagaga aatctctata cttagaagtt tgataagaat ttaaaagtat
      601 cactttataa tcagcatcag taatataact atagaaattt tcactaaatg ctccagttga
      661 ttctatacca ataataaagt cattcatatc taatgatagt tcagctaatc gtttcagtag
      721 ttctcttaat cccaatatat cactataaat ccaaaaaggt ttgcaagcta actcttttgc
      781 atcatccaaa atacaaacat agtgtttttc ttttgcaata tcaactccaa cataatacat
      841 aataatcctc cataatgtga tagtttaaaa tctcatagct tcgtgttata acaggtagaa
      901 aatatttttc tcccgagcat cctatagatg atttaataaa gctaacacaa gacgttttat
      961 atgtaggtat ttaaatccta aaaggaaaaa aacttgttta ttattagcct ttgtgagagc
     1021 tatgagtttc atagaacttc aatcaatatt ttaaactttg attgataaat gtattatacg
     1081 aaggaaaaaa atggactggg gtaaggtaac ctatattttc ttaacattaa tgtct
//
LOCUS       DAOQAN010000532         1133 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_206115_length_1133_cov_1.827458, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000532 DAOQAN010000000
VERSION     DAOQAN010000532.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_206115_length_1133_cov_1.827458"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="PKO10_07615"
     CDS             <1..262
                     /locus_tag="PKO10_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW67052.1"
                     /translation="YEQNLPFVDGGLYAMNLLYSLHYNGLATIPLTMGHKWRVIKKIK
                     QEMNIPGNEIPVLLIGVGTYKDDFKVAVSHRYSYKQYVKFNQ"
     gene            complement(423..>1133)
                     /locus_tag="PKO10_07620"
     CDS             complement(423..>1133)
                     /locus_tag="PKO10_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4010 domain-containing protein"
                     /protein_id="HNW67053.1"
                     /translation="WLTVVVISTISYISYLVQKYIFPSKGVLLTGILGGAYSSTATTV
                     VLSKKAQAVQDTNMFTSSIISATFMMYLRLLIIAAIFNIEVLKVIATPYIVFGLITLI
                     ISFVYYKKATNETSKIETEDRNPLELGTAFVFAILFIVTMFITNFVVNNYGTTGLNIL
                     SFIIGMTDIDPFILALLTGKYNIDAVAVASAMLIATGSNNILKALYAVWFGKKKAVSS
                     AFWLIILGFLTIGFGIYI"
BASE COUNT          440 a          169 c          147 g          377 t
ORIGIN      
        1 ttatgaacaa aatctacctt ttgttgatgg aggactttat gcgatgaatc ttctttactc
       61 ccttcattat aatggattag ctaccatacc attgactatg ggacataaat ggagagtcat
      121 aaaaaagata aagcaagaaa tgaatattcc cggaaatgag attccagttc ttttgatagg
      181 tgttggaacg tataaggatg actttaaagt tgcagtttcg catcgctatt catataaaca
      241 gtatgtaaaa tttaatcaat aagtgatgag tatagtgttt tagttaaact tctgctgtaa
      301 gttttatact catcaattat caatcgaaaa tatggtggat tataacaaaa gtttatttta
      361 tttacattta ttttaaaaac ttatttaaaa atttaaacaa aatgtaacaa taagtaaaaa
      421 tattaaatat atataccaaa acctattgtc aaaaatccta aaataattaa ccaaaaagca
      481 cttgaaactg cttttttctt accaaaccaa actgcatata gtgcttttaa tatattgtta
      541 cttccagtag caattaacat cgcacttgca accgcaactg catcaatatt atattttcca
      601 gtcaataaag ccaaaataaa agggtctata tctgtcattc ctataataaa agataagata
      661 tttaatcctg ttgttccata attatttact acaaaatttg taataaacat agtaacaata
      721 aataaaattg caaatacaaa agcagttccc aactccaaag gatttctatc ttctgtctca
      781 atttttgaag tttcatttgt tgctttttta taataaacaa aagatataat caaagtaata
      841 agcccaaaaa ctatataagg tgtagcaata actttaagca cctcaatatt aaaaatcgca
      901 gctattataa gaagtcgtaa atacatcata aaagttgcac ttattataga agatgtaaac
      961 atatttgtat cttgtactgc ttgtgctttt tttgataata caactgtagt agcagttgaa
     1021 gagtaagcac cacctaaaat accagtcaat aaaacacctt ttgatgggaa aatatatttt
     1081 tgaaccaaat agcttatata tgagattgta gagattacaa caaccgtaag cca
//
LOCUS       DAOQAN010000533         1130 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_207045_length_1130_cov_1.509767, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000533 DAOQAN010000000
VERSION     DAOQAN010000533.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_207045_length_1130_cov_1.509767"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="PKO10_07625"
     CDS             <1..574
                     /locus_tag="PKO10_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014469066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HNW67054.1"
                     /translation="EEIIQNSSLENQMSMKYIKIDENKNYIRAKNEDAGRFRNEEFFK
                     KIYGKNRQEIEKNLVKIKWLEKSQNKTLWVTKINGIDKKLEKISSELENLPKKFKKYI
                     VNPDGVYNFRKISGTNRLSTHSFAIAIDLNKEYSNYWLWDKKGKNIEYKNKIPLEIVK
                     IFEKYGFIWGGRWYHYDTMHFEYRPELLLD"
     gene            complement(566..865)
                     /locus_tag="PKO10_07630"
                     /pseudo
     CDS             complement(566..865)
                     /locus_tag="PKO10_07630"
                     /inference="COORDINATES: protein motif:HMM:NF015909.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
     gene            complement(871..>1130)
                     /locus_tag="PKO10_07635"
     CDS             complement(871..>1130)
                     /locus_tag="PKO10_07635"
                     /inference="COORDINATES: protein motif:HMM:NF015422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helicase associated domain-containing protein"
                     /protein_id="HNW67055.1"
                     /translation="EALSLFYSEEGINRLNEVGLIWNPIESLWDKNFTELCRFKGING
                     HCNVPQNYPENSSLAGWVCSNRNLYQKKSKQEDLKREKKIL"
BASE COUNT          434 a          160 c          159 g          377 t
ORIGIN      
        1 tgaagaaata attcaaaata gctctttaga aaatcaaatg agtatgaaat atataaaaat
       61 agatgaaaat aaaaactata tccgagcaaa aaatgaagat gctggaagat ttagaaatga
      121 agagtttttc aaaaagatat atggtaaaaa tagacaagaa atagaaaaaa atcttgtaaa
      181 aataaagtgg ctagaaaaaa gtcaaaataa aactttatgg gttacaaaaa taaacggtat
      241 agataaaaaa ctagaaaaaa taagtagtga attagaaaat ctaccaaaaa agtttaaaaa
      301 atatatagta aatccagatg gagtttataa ttttagaaaa attagtggta caaataggct
      361 tagcacccat agttttgcaa tagctattga tttaaataaa gagtattcaa actattggct
      421 ttgggataaa aaaggaaaaa atattgagta taaaaacaaa attcctcttg aaattgtaaa
      481 aatatttgag aaatatggtt ttatttgggg agggcgatgg tatcattatg ataccatgca
      541 ctttgagtat agacctgagt tacttttaga ttaaaccttg agtgattcct gaatttttct
      601 tataatccat aatttcaagc tctttttcag cagtaatcac aatatcacta tcaactgaga
      661 aatctaacct ttcgtctctt ccttcataag gaattgagtt taaaataact tttatagagt
      721 taagtctagc caaaaattta ctatcacttc ttataattgt ccaaggtgat tttggagtat
      781 ttgtgtactt taacatatca tatttttttt gggtaaactc ttcccaccta ctttgcattt
      841 gcatatcaat ttcacttagt ttccactgtt ttagaggatt ttcttctctc tcttcaaatc
      901 ttcttgcttg ctcttctttt gatacaaatt acgattagaa caaacccacc cagcaagaga
      961 cgaattttcg ggataattct gaggtacatt gcaatgtcca tttattcctt taaacctaca
     1021 taactcagta aagtttttat cccataagct ttctataggg ttccaaatca atccaacttc
     1081 atttaatcta ttaatgcctt cttcactata aaacaatgat aatgcttcaa
//
LOCUS       DAOQAN010000534         1129 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_207162_length_1129_cov_2.733706, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000534 DAOQAN010000000
VERSION     DAOQAN010000534.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1129
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_207162_length_1129_cov_2.733706"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="PKO10_07640"
     CDS             <1..445
                     /locus_tag="PKO10_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dehypoxanthine futalosine cyclase"
                     /protein_id="HNW67056.1"
                     /translation="MMFGTVETDEEIIEHWDLIRKLQDETGGFRAFIMWSFQGENTKL
                     MEEHPLIKPQSSNRYLRLLAVSRLYLDNFKNLQSSWVTQGSYIGQMALKFGANDLGST
                     MMEENVVKAAGAANRMNQDEMIRLIKDIGEIPAKRDTAYNILERF"
     gene            501..>1129
                     /locus_tag="PKO10_07645"
     CDS             501..>1129
                     /locus_tag="PKO10_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014474250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HNW67057.1"
                     /translation="MSATIKHININNVDIPVIFEEQKSLPILNLQLIFKNSGYIKDNQ
                     NLGLASLSARVLNEGTKKLGSVKFSEILDDNAITIHSAVGFETLTIELSSLKEKSNLA
                     IKSLNELLQSPNLSENSLEKVKTLAIGSLKRKENDFDDVASKGLNALLYSGTALANPA
                     SGTIESISKIELKNIEQFLKNSLTLNNLTIVAGGDISFIELENALKPLL"
BASE COUNT          452 a          128 c          185 g          364 t
ORIGIN      
        1 aatgatgttt ggaactgttg aaacagatga agagattatt gaacattggg atttgattag
       61 aaaattacaa gatgagactg gtggatttag agcatttatt atgtggagtt ttcaaggtga
      121 aaatacaaaa ttaatggaag aacatcctct tataaaacca caatcttcaa atagatattt
      181 aagactctta gcagtttcaa gattatattt agacaatttt aaaaatcttc aaagctcttg
      241 ggttactcaa ggctcataca ttgggcaaat ggctcttaag tttggagcaa atgatttagg
      301 tagcactatg atggaagaga atgttgtaaa agcagctggt gcagcaaata gaatgaatca
      361 agatgaaatg ataagattaa taaaagatat aggcgaaatt cctgcaaaaa gagatactgc
      421 ttataatata ttagagagat tctaacaaaa tgaaaacaag atatatttta tatatttttt
      481 tactattaca aggaagttta atgagtgcaa caataaaaca tataaatata aacaatgttg
      541 atattccagt gatttttgaa gaacaaaaat cgcttccaat tttaaattta caactaattt
      601 ttaaaaattc tggatatata aaagataatc aaaatttagg acttgctagc ttatcagcta
      661 gagttttaaa tgaaggaaca aaaaaattag gaagtgttaa attttcagag attttagatg
      721 acaatgctat tactattcat agtgctgttg gttttgaaac tcttacaata gaactttcaa
      781 gcttaaaaga gaaatcaaat cttgcaatta aatcattaaa tgaactttta caatctccaa
      841 atttatcaga aaattcatta gaaaaagtaa aaactttggc tattggtagt ttaaaaagaa
      901 aagagaacga ctttgatgat gtagcaagta agggtttaaa tgctcttctt tatagtggaa
      961 ctgctttggc aaatcctgct agtggaacaa tagaatctat ttcaaaaatt gaactaaaaa
     1021 atatagaaca atttttaaaa aactctttaa ctttaaataa tttaactatt gttgcaggtg
     1081 gagatattag cttcattgag ctagaaaatg ctttaaaacc tcttttaaa
//
LOCUS       DAOQAN010000535         1127 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_207880_length_1127_cov_1.733209, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000535 DAOQAN010000000
VERSION     DAOQAN010000535.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_207880_length_1127_cov_1.733209"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="PKO10_07650"
     CDS             <1..687
                     /locus_tag="PKO10_07650"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNW67058.1"
                     /translation="SKNIYKFILSKSTKTPSSMEDSMVGHLRVYGNKDLKNEEIESAE
                     LIYIYSDNKDKLKLNLFYSIYKNGIDSREFDTNKYKFINKSDDENNYGLELEYSKLFE
                     NRSKLFLNSSYNIFEYKNSYYNFDINTPVVSKLTTTLGFIYPLSSKFTISPLLRYYGD
                     KNLLNGTEIGDVLLTDLTLSYRFSKDSKLSFGAKNLFDQNYYYYGNKTKDEKMLREGR
                     TWFTSFSYDF"
     gene            677..>1127
                     /locus_tag="PKO10_07655"
     CDS             677..>1127
                     /locus_tag="PKO10_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW67059.1"
                     /translation="MISKKIFFIIFLQFSLFANSVYNINFLDFQAKVFPKIILLDNNF
                     EDKLLENSVVLTILYEEIDYRVVEILKDKIEKEYKSLKDYNLKVNSLKYSEFNRNKLS
                     TAYILLLGKDEKILEISKILSSNNRLTFAYDNSYLDLGVIFGLAITSK"
BASE COUNT          469 a          103 c          149 g          406 t
ORIGIN      
        1 agtaaaaata tatataaatt tattctctca aaatctacga aaacaccttc ttctatggaa
       61 gattcaatgg ttgggcatct aagagtttat ggtaataaag atttaaaaaa tgaagagata
      121 gaaagtgctg aattaatata tatttacagt gataacaaag ataaacttaa attaaatcta
      181 ttttattcta tctataaaaa tggtattgat tcaagagagt ttgatacaaa taaatataaa
      241 tttattaata aaagtgatga tgaaaataat tatggtttag agttagagta ttcaaaacta
      301 tttgaaaatc gttcaaaact atttttaaat agtagctata atatttttga atataaaaat
      361 agttattata attttgatat taatacacct gttgtatcta aacttacaac tactttagga
      421 tttatttatc ctttaagttc aaaatttacg ataagtccac ttttaaggta ttatggagat
      481 aaaaatctat taaatggaac agagattggt gatgttttat taacagattt aactctatca
      541 tataggttct caaaagactc taaactatct tttggagcta aaaatctatt tgaccaaaac
      601 tactattact acggaaataa aactaaagat gagaagatgc taagagaagg aagaacttgg
      661 tttacaagtt ttagttatga tttctaaaaa aatatttttc ataatattct tacaattttc
      721 actctttgca aatagtgtat ataatataaa ttttttagat tttcaagcaa aagtatttcc
      781 aaaaattata ttactagaca ataattttga agataagcta ttagaaaata gtgtagttct
      841 tacaatttta tatgaagaga tagattacag ggtagtagaa attttaaaag ataagataga
      901 aaaagagtac aaatctttaa aagattataa tcttaaagta aatagtttaa aatatagtga
      961 atttaataga aataaattaa gtacagcata tattttatta ttaggaaaag atgaaaaaat
     1021 attagaaata tctaaaatct tatcttctaa taataggctt acttttgctt atgataatag
     1081 ctatttggat ttaggagtta tttttggact agccataact tcaaaag
//
LOCUS       DAOQAN010000536         1126 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_207983_length_1126_cov_3.154062, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000536 DAOQAN010000000
VERSION     DAOQAN010000536.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_207983_length_1126_cov_3.154062"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="PKO10_07660"
     CDS             complement(<1..446)
                     /locus_tag="PKO10_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014467910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNW67060.1"
                     /translation="MAISSNLTLELLNQPFLLEKRIELLKAIKQTGSINKAAALVPMS
                     YKSAWEAVEAMNNLSISPIVTRETGGAGGGGTTLTQYGENLLTTYSLLKEEQRKFIEN
                     LNRITDLNSGTLKTIKRLSMQISARNQIIGTIEKISLGAVNAEIQM"
     gene            547..>1126
                     /locus_tag="PKO10_07665"
     CDS             547..>1126
                     /locus_tag="PKO10_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW67061.1"
                     /translation="MPKLNIKNITLIFYDILKQGTNMQIHELLLHILPVVIFSFIIGL
                     EVRAYLTKFHENDARVFFGTTRTYAFLGILGFILYEIEPKNFSLFIVGFLSITFLYSI
                     LYKKMLDTDKNSILLYIVSIIVYSFGPLIGIFPLWISALIFVLVIFLLNSKNRILTFH
                     PKINIYELETFGKIILLSAVVLPLLPTDNNIPY"
BASE COUNT          363 a          156 c          150 g          457 t
ORIGIN      
        1 ttcatttgaa tttcagcatt aactgcaccc aatgaaattt tctcaatagt cccaattatt
       61 tggtttcttg cacttatttg cattgatagc ctctttatag tttttagtgt tccactattt
      121 aaatcagtaa ttcggtttag attttcaata aacttccttt gctcctcttt taataaacta
      181 taagttgtca aaagattttc accatattgc gtaagagtag ttccaccacc accagctcca
      241 cctgtttctc ttgtaactat tggtgaaatt gatagattat tcatagcttc aacagcttcc
      301 caagcacttt tgtaactcat aggaacaaga gctgctgctt tgtttattga gcctgtttgt
      361 ttaatagctt ttagaagttc aattcttttt tcaagtaaaa aaggttgatt taaaagctct
      421 aaagtcaaat tagatgatat tgccacattt agtccttatg ttatatttta agatatataa
      481 cgaatgggaa tattagcttt atatacctta agaaataatg attaattttt taaaaattat
      541 tgtattatgc caaaattaaa tattaaaaat ataactctta tattttatga tattttaaaa
      601 cagggaacaa atatgcagat acacgagctt ttattacaca ttcttccagt agtgattttt
      661 agttttataa taggcttgga agtaagagcc tatttgacaa aatttcatga aaatgatgct
      721 agagtttttt ttggaactac aagaacttat gcttttttgg gaattttagg atttatttta
      781 tatgagatag agccaaagaa tttttcactt tttatagttg gatttttgtc tattactttt
      841 ttatactcaa ttttatataa aaaaatgcta gatacagata aaaatagcat acttttatat
      901 atagtttcaa taattgtata tagtttcgga cctcttatag gaatatttcc tctttggata
      961 agtgctttga tatttgtatt ggttatattt ttgttaaatt caaaaaatag aattttaact
     1021 ttccatccaa aaataaatat ctatgagtta gagacttttg gtaaaattat tttgctttca
     1081 gcagttgttt tgccacttct tccaactgat aacaatattc catatt
//
LOCUS       DAOQAN010000537         1121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_209360_length_1121_cov_13.469043, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000537 DAOQAN010000000
VERSION     DAOQAN010000537.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_209360_length_1121_cov_13.469043"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="PKO10_07670"
     CDS             <1..595
                     /locus_tag="PKO10_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW67062.1"
                     /translation="KVNWHNISIYQKLSEEFIKEFSDKVNWYNISIYQKLSEEFIKEF
                     SDKVNWYYISEYQKLSESFIKEYNLTIPKDNWLYKTKEDKLSYLKENCNGIYEIVDDN
                     YIIAYKSTRFDGHSTFNFQYQYKENETYETHCDCNIDNEISFGLSAWTKEKALAHYSK
                     GKLFKVYINIEDIGAIIHDNNKIRCSKLIIVSEESFE"
     gene            607..>1121
                     /locus_tag="PKO10_07675"
     CDS             607..>1121
                     /locus_tag="PKO10_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW67063.1"
                     /translation="MNSQVFEEMKLIAKDYNIDFSDENDIKICKKIKWDCISKYQKLS
                     EKFIREFSDRVGWHYISAYQKLSEGFIREFADKVNWINISIYQKLSEGLIREFADKVN
                     WINISQYQKLSEEFIREFSKKVDWYHISINQKLSEEFIREFADKVNWYCISKYQKLSK
                     EFIKEYNLTIS"
BASE COUNT          492 a           93 c          156 g          380 t
ORIGIN      
        1 taaagtaaat tggcataata tttcaatata tcaaaaacta tcagaagaat ttattaaaga
       61 attttctgat aaagtaaatt ggtataatat ttcaatatat caaaaactat cagaagaatt
      121 tattaaagaa ttttctgata aagtaaattg gtattatatt tcagaatatc aaaaactatc
      181 agaatcattt attaaagaat acaatttaac aataccaaaa gataattggt tatacaaaac
      241 aaaagaagat aaattatctt atttgaaaga aaattgcaat ggtatttatg aaattgtaga
      301 tgataattat attattgcat ataagtcaac aagatttgat ggacattcta cattcaattt
      361 tcaatatcaa tataaggaaa atgaaacata tgaaactcat tgtgattgta atatagataa
      421 tgagatttca tttggattat cagcatggac aaaagaaaag gcattggcac attatagtaa
      481 agggaaacta ttcaaagtat atattaatat agaggatatt ggagcaatta ttcatgataa
      541 caataaaatt cgttgtagta aattgataat tgtttctgaa gaatcttttg aatagaaaga
      601 aatataatga atagtcaagt ttttgaagaa atgaaactaa ttgcaaaaga ttataatatt
      661 gatttttcgg atgaaaatga tattaaaata tgtaagaaaa taaaatggga ttgtatttca
      721 aaatatcaaa aactatcaga aaaatttatt cgagaatttt ctgatagagt aggttggcat
      781 tatatttcag cttatcaaaa actatcagaa ggatttattc gagaatttgc tgataaagta
      841 aattggatta atatttcaat ttatcaaaaa ttatcagaag gattgattcg agaatttgct
      901 gataaagtaa attggattaa tatttcacaa tatcaaaaac tatcagaaga atttattcga
      961 gaattttcta agaaagtaga ttggtatcat atttcaatta atcaaaagtt atcagaagaa
     1021 tttattagag aatttgctga taaagtaaat tggtattgta tttcaaaata tcaaaaacta
     1081 tcaaaagaat ttattaaaga atacaattta acaatttcaa g
//
LOCUS       DAOQAN010000538         1119 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_210158_length_1119_cov_2.233083, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000538 DAOQAN010000000
VERSION     DAOQAN010000538.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1119
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_210158_length_1119_cov_2.233083"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            78..>1119
                     /locus_tag="PKO10_07680"
     CDS             78..>1119
                     /locus_tag="PKO10_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW67064.1"
                     /translation="MSNNHFIWDSYSDQPQVIKDRAFKKATRRKELKDNLKLLFTSIF
                     ILPISIIIMKFFKGNFKTSNIDFIGLGVNLDKDDGKNTQQDLVQELGVKNLIIRLPLS
                     DIKNIDLYFEFANSFNKNERKNILINVIQDRLNIENQELFKKNIDLIFQKFENISNEF
                     QIGTTINRLKWGFFSTEEFMNFYMVASKIKEDKYPNIKLLGPSVIDFEYYYNARAMFN
                     LKKIKYEITSSLLYVDRRGAPQNSQYGIFDLKNKIDMLFSLVKMSPKTLSDDIYITEV
                     NWPISNTAPYAPTSEKECVSCDDYTKYMLDYFKIAQDSRKIKRVYWHQLIASGYGLVD
                     NRDGKILKYPQFY"
BASE COUNT          470 a          104 c          163 g          382 t
ORIGIN      
        1 aaataatttt atgataaata aaaattatac aaaagagcaa tatttgcaaa tagaaaatag
       61 gttaaaagag attttggatg tcaaataacc attttatttg ggatagttat tcagatcaac
      121 ctcaagttat caaggataga gcttttaaaa aagctacaag aagaaaggag ttaaaagata
      181 atttaaaatt actttttact tctattttta ttcttcctat ttcaataatt attatgaaat
      241 tttttaaagg aaattttaaa acttcaaata tagatttcat aggtcttgga gtaaatctag
      301 ataaagatga tggtaaaaat acccaacaag atttggttca agagttgggt gtaaagaatt
      361 taattattag attacctctt agtgatatta aaaatattga tttatatttt gagtttgcaa
      421 atagttttaa taaaaatgag agaaaaaata ttttaataaa tgtaattcaa gatagattaa
      481 atattgaaaa tcaagaactt tttaaaaaaa atattgattt gatttttcaa aagtttgaaa
      541 atatatcaaa tgagtttcaa attggaacta caataaatag attaaaatgg ggatttttta
      601 gtacagaaga atttatgaat ttttatatgg tagcttccaa aataaaagag gataaatatc
      661 caaatattaa acttttagga ccatcagtta tagattttga gtactactac aatgcaagag
      721 ctatgtttaa tttaaaaaaa ataaaatatg agataacttc atctttactt tacgtagata
      781 gaagaggtgc tccacaaaat agtcaatatg gaatatttga tttaaaaaat aagatagata
      841 tgcttttttc acttgtaaaa atgagtccaa aaactttaag tgatgatatt tatattacag
      901 aagttaattg gccaatatct aatacagctc catacgcacc tacaagtgaa aaagagtgtg
      961 ttagctgcga tgactacaca aaatatatgt tggattattt taaaatagca caagattcaa
     1021 gaaaaataaa aagagtttat tggcatcaac taattgctag cggatatgga ttagttgata
     1081 atagagatgg aaaaatttta aaatatcctc aattttatg
//
LOCUS       DAOQAN010000539         1117 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_210757_length_1117_cov_2.327684, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000539 DAOQAN010000000
VERSION     DAOQAN010000539.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_210757_length_1117_cov_2.327684"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1066)
                     /locus_tag="PKO10_07685"
     CDS             complement(<1..1066)
                     /locus_tag="PKO10_07685"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNW67065.1"
                     /translation="MVSKSVVELISLYELNPIKAFFTVYLPLSYPAIFAGTILAIMET
                     LSDYGTVLYFGIETFSVGVFKSWFGYGDLGGAINIAIILLVFVFFILLVEYNIRKKLK
                     FSSSTNSSEKPSKIKLKGKYNFFAFLISFIIALFTLFIPTGVLIYWTLLDINTLDFTA
                     FEYLFNSLKLNIISSSFIISLAFFIVYFLRFFSTKVSSFTHKVSMLGYSIPGAVVAVG
                     LLLIANYLDRGLGFMFFSGSFIILIFAYTTRYFAASIGSVENGFSKINSSLDDTSRIF
                     CKNEKESIFKIYLPILKPYLLSGFLILYIDIAKELPATLILRPFNFDTLAVRIYELAS
                     NEMLYKTGFPSLLLVITTASA"
BASE COUNT          468 a          170 c          139 g          340 t
ORIGIN      
        1 cagcactagc agttgtaata accaaaagaa gtgatggaaa accagtttta taaagcatct
       61 cattacttgc taattcataa attcgaaccg ctagtgtatc aaaattaaac ggtcttaaaa
      121 tcaaagttgc tggaagctct tttgcaatat cgatatataa aattaaaaaa ccactaagca
      181 aatatggttt taaaatagga agatatattt taaagataga ctctttttca tttttgcaaa
      241 atattcggct tgtatcatct agtgagctat ttatcttact aaaaccgttt tcaacacttc
      301 ctatacttgc tgcaaaatat cttgtagtat atgcaaaaat taagattata aatgaaccac
      361 taaaaaacat aaaacccaat ccacggtcta aataatttgc tatcaaaagt agtccaacag
      421 ctacaacagc accaggaata gagtatccta gcattgaaac tttgtgagta aagcttgata
      481 cttttgtaga aaaaaatctc aaaaaatata caataaaaaa agccaaagat attataaaac
      541 tactagaaat tatatttaat tttaaactat taaatagata ctcaaatgcc gtaaaatcaa
      601 gagtgtttat atccaaaaga gtccaataaa tcaaaacccc agtaggtata aaaagtgtaa
      661 aaagagcaat tataaaagat attaaaaatg caaaaaagtt atattttcct tttaatttta
      721 ttttagaagg cttctcgcta ctgtttgttg agctagaaaa cttgagtttt tttctaatat
      781 tatattctac taatagtata aaaaatacaa aaacaagtaa aataattgca atatttatag
      841 ctccacctaa atcaccataa ccaaaccaag atttaaaaac tccaacacta aaagtctcta
      901 ttccaaagta taaaacagtt ccataatcgc ttaaagtctc cataatagct agtattgttc
      961 ctgcaaaaat tgctggatat gataaaggaa gataaacagt gaaaaatgct tttatcggat
     1021 ttaattcata taaagatata agttctacaa ctgattttga taccatagaa aaagatacac
     1081 gagctagtat aaaaacatat ggaaacattg caattgt
//
LOCUS       DAOQAN010000540         1115 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_211289_length_1115_cov_2.952830, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000540 DAOQAN010000000
VERSION     DAOQAN010000540.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_211289_length_1115_cov_2.952830"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="PKO10_07690"
     CDS             complement(<1..623)
                     /locus_tag="PKO10_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin fold domain-containing protein"
                     /protein_id="HNW67066.1"
                     /translation="MFRILKNVIFLSLLLVLNLEAKEKELTNKQMLEFESLELFKKAG
                     IKVEKAYDIGSLYLLSIVVQGNKDEIFLTKDKNYLISGAVINTTTGMQIKAPVNLAIL
                     KDKEAFTYGNGKDEYTLFTDPECSYCKKFESFLPQIKDKVKIKVFFFPLDFHENAKDL
                     SIYIMSKKTRDEKINAMFEFNIGDNLSKVKNAKYSKNELEKLEKKLE"
     gene            complement(681..1061)
                     /locus_tag="PKO10_07695"
                     /pseudo
     CDS             complement(681..1061)
                     /locus_tag="PKO10_07695"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
BASE COUNT          353 a          182 c          147 g          433 t
ORIGIN      
        1 tcttcaagct ttttctctag tttttctaac tcatttttag agtattttgc attttttact
       61 ttacttaaat tatcgccaat attaaattca aacattgcat taattttttc atctcttgtt
      121 tttttactca taatgtatat acttaaatct tttgcatttt catgaaagtc taaagggaag
      181 aaaaacactt ttatttttac tttatctttt atttgtggta aaaatgactc aaactttttg
      241 caatatgaac actctggatc tgtaaaaaga gtatactcat cctttccatt tccataagta
      301 aaagcctctt tatcttttaa aatagctaag tttacaggag cttttatttg cataccagtt
      361 gttgtattta taacagcacc tgatataaga tagtttttat cttttgttaa gaaaatttca
      421 tctttatttc cttgaacaac tattgaaaga agatataagc ttcctatatc gtaagctttt
      481 tcaactttta taccagcttt tttaaacagt tctaagcttt caaactctag catctgtttg
      541 tttgttagct ctttttcttt tgcttctagg tttaaaacta gtaacaaaga tagaaatatt
      601 acatttttta atattctaaa cattcttttc cttaaaattt ttttgcaatt cttactaaaa
      661 tttggttaat agttgatgaa ttacattatt aagattttaa ctagatatgt tgtaacaaat
      721 gatattaaaa ttccataacc aactattgca gagcttagtc ttggagcaag tccagccatt
      781 gctgctattg cacctgctgt tatcattgga gccatagcag cttctagtat tgatacagtt
      841 cctgcaagat tattccatcc aaaaatttta caaacaataa ttgcaataat tggagcaaaa
      901 ataagtttaa caaataaagc tacactaaaa ggttttatat cctcttttgg aagtctaagt
      961 tgaagttgaa gtccagctgc tacaagtgca actggaacag ttgttagggc aaaagcctct
     1021 aatacttttg taattgtagg attaaactcc actccaacta aaaatagagc aaaaactagc
     1081 gtttttcagc cggaggctgg tgcccgccgg tttat
//
LOCUS       DAOQAN010000541         1115 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_211471_length_1115_cov_1.556604, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000541 DAOQAN010000000
VERSION     DAOQAN010000541.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_211471_length_1115_cov_1.556604"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /gene="actP"
                     /locus_tag="PKO10_07700"
     CDS             <1..298
                     /gene="actP"
                     /locus_tag="PKO10_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012309.1"
                     /note="member of the sodium:solute symporter family;
                     cotranscribed with the acs gene which encodes acetyl
                     coenzyme A synthase; mutations affect acetate uptake;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HNW67067.1"
                     /translation="YWSKLTTRGAFIGGFMGLLTAVILVVLSKTVWVDIFGFKDAIFP
                     WTHPALFSVSAAFIAIWFFSITDSSARAEEDKSGFEAQKIRGETGIGSDGAVSH"
     gene            526..828
                     /locus_tag="PKO10_07705"
     CDS             526..828
                     /locus_tag="PKO10_07705"
                     /inference="COORDINATES: protein motif:HMM:NF016244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HNW67068.1"
                     /translation="MDEKLVEKIEANPKYKELVSKRNSLALKLGIFVLIMFYSFITVV
                     AFNKDLFAIKVAEGYITTIAFPIALAILVISFLTTLVYVRRANGEFDDLQMILKKM"
     gene            839..>1115
                     /gene="actP"
                     /locus_tag="PKO10_07710"
     CDS             839..>1115
                     /gene="actP"
                     /locus_tag="PKO10_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012308.1"
                     /note="member of the sodium:solute symporter family;
                     cotranscribed with the acs gene which encodes acetyl
                     coenzyme A synthase; mutations affect acetate uptake;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HNW67069.1"
                     /translation="MIRIITLLFLASLALFAAGDATFEGKRELNVAAIAMFLVFIVGT
                     LGITYWAAKRTKSASDFYTAGGGITGFQNGLAIAGDYMSAAAFLGVSG"
BASE COUNT          382 a          133 c          194 g          406 t
ORIGIN      
        1 ttattggtca aaattaacaa caagaggtgc atttattggt ggatttatgg gattactaac
       61 agctgttatt ctagttgtac taagtaaaac tgtatgggtt gatatttttg gtttcaaaga
      121 tgctatattc ccatggacac atccagcact attctctgta tctgcagcgt ttattgctat
      181 ttggttcttc tctataactg atagttcagc tagagcagaa gaagataaat caggatttga
      241 agctcaaaaa attagaggtg aaactggtat tggttctgac ggagctgtat cacactaata
      301 aaattactaa agagagaaat ctctttagta ataacttaaa aaatacttaa taatcataat
      361 taattgtaat aattaatata ttgttttttg ttactattct acatttattt taaaatttct
      421 atataatttt tatcaacaaa tcaagcaaaa tataaattta tttgtataat cgattacttt
      481 atctataatt tagataaaaa taaaaaagtc gcaggagaat aaggtatgga tgagaaattg
      541 gttgagaaaa tagaagccaa tcctaaatat aaagagctag tttcaaaaag aaactcttta
      601 gctttaaaac taggaatttt tgtgttgata atgttttatt catttatcac agtagttgca
      661 tttaacaaag atctatttgc aataaaagta gcagaaggtt atataacaac tattgcattt
      721 cctattgcat tagcaatttt agttattagc tttttaacaa cactagttta tgtaagacgt
      781 gcaaatggtg agtttgatga tctacaaatg atattaaaaa agatgtaagg gagtttttat
      841 gattagaata ataactctac tttttttagc ttcacttgct ctttttgctg caggtgatgc
      901 aacttttgaa ggtaaaagag aattaaatgt agctgctatt gctatgtttt tagtttttat
      961 tgttggaaca ctaggaatta catattgggc cgctaaaaga acaaaatctg ctagtgattt
     1021 ttatacagct ggtggtggaa taactggttt tcaaaatggt ctagcaattg ctggtgacta
     1081 tatgagtgca gctgcatttt taggtgtttc tggac
//
LOCUS       DAOQAN010000542         1113 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_211886_length_1113_cov_3.142722, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000542 DAOQAN010000000
VERSION     DAOQAN010000542.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_211886_length_1113_cov_3.142722"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="PKO10_07715"
     CDS             complement(<1..480)
                     /locus_tag="PKO10_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012139940.1"
                     /note="catalyzes the formation of
                     2-methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at
                     position 37 in tRNAs that read codons beginning with
                     uridine from N6-(dimethylallyl)adenosine (i(6)A); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HNW67070.1"
                     /translation="MSKKLFIQTLGCQMNDTDSKHIQAELEKHKGYSATQNIEDADLI
                     IINTCSVREKPVQKLFSEIGQFNKKKKDGAKIGVCGCTASHLGEDIIKRVPYVDFVLG
                     ARNISKIKDVVDKKGSVEISIDNDDSQYEFAIANNNNFRTSVNISVGCDKECTYCIVP
                     "
     gene            complement(502..744)
                     /locus_tag="PKO10_07720"
     CDS             complement(502..744)
                     /locus_tag="PKO10_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002851103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HP0268 family nuclease"
                     /protein_id="HNW67071.1"
                     /translation="MELLIARNELNEKPKKVQLEKIKDELKKEGQKIFYFDRDNSHKD
                     MMALVEALEKEGFNIYFREIKFGLADDEYMYEVHAL"
     gene            897..>1113
                     /locus_tag="PKO10_07725"
     CDS             897..>1113
                     /locus_tag="PKO10_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NusA N-terminal domain-containing protein"
                     /protein_id="HNW67072.1"
                     /translation="MDKIIDILDSIAYEKGLKITDVENALKEALIKTAQKMVDYTLIF
                     DAKVDRAAKKLTLFQKIEVVANDDKRLF"
BASE COUNT          381 a          176 c          125 g          431 t
ORIGIN      
        1 aggaactatg caatacgtac actctttatc acaaccaaca gaaatattta cacttgttct
       61 aaaattgttg ttatttgcta ttgcaaactc atattgtgag tcgtcattat caatacttat
      121 ttcaacagag ccttttttat caacaacatc ttttattttt gaaatatttc tagctcccag
      181 tacaaaatca acataaggaa ctctttttat aatatcttca cctaaatgag atgctgtgca
      241 tccacaaaca ccaatttttg caccatcttt ctttttctta ttaaattgtc caatttcaga
      301 aaatagtttt tgaactggtt tttctcgaac agaacaagtg tttataataa ttaaatcagc
      361 atcttcaata ttttgtgtag cactataacc tttgtgtttt tcaagttctg cttgaatatg
      421 tttactatcg gtgtcattca tttgacaacc taaagtttgt ataaatagtt ttttactcat
      481 attttttatc actataaaat attaaagtgc atgaacctca tacatatact catcatcagc
      541 taatccaaat ttaatctctc taaaatagat attaaatccc tctttttcta aagcttcaac
      601 tagagccatc atatctttgt gagagttatc tctatcaaag taaaatattt tttgaccctc
      661 tttttttagc tcatctttaa tcttttccaa ctgtactttt tttggctttt catttaattc
      721 atttctagca attaataatt ccatttcatt aaaccttttt tttcaaataa tcgtatattt
      781 tagtcaaaaa ttgctttaat ttctataaaa ggtagttttg atatactatt gtcctaaaac
      841 ttgcattgaa aatcaaactt accatttcaa tgttaaaaaa actataggaa ataattatgg
      901 ataaaataat agatatttta gactcaatag cttatgaaaa gggtcttaaa attactgatg
      961 tagaaaatgc tctaaaagag gctttgatta agactgctca aaaaatggtt gattacactt
     1021 tgatttttga tgcaaaagta gatagagcag ctaaaaaatt aacacttttt caaaagatag
     1081 aagttgtagc aaatgatgat aaaagattat ttg
//
LOCUS       DAOQAN010000543         1113 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_212015_length_1113_cov_2.165406, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000543 DAOQAN010000000
VERSION     DAOQAN010000543.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_212015_length_1113_cov_2.165406"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1113
                     /locus_tag="PKO10_07730"
     CDS             <1..>1113
                     /locus_tag="PKO10_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNW67073.1"
                     /translation="VIISGQVPTTIIGTDGFQEIDAVGISRPCTKHNYLVNKIEDLPR
                     IIKEAFHLASTGRPGPVHVDIPKDITAQIGEFIYPSEVNMPTYKPTLNYNKKQLKRAM
                     EAISNAKKPLLYIGGGAILSNCGYEIRDLAKKLNIPAVETLMARGVMGDENPLFFGML
                     GMHGEYAANMAAHETDLLISLGARFDDRVTGRLDEFASKAKVIHIDIDPTSIAKLVVA
                     DYPIVGDLKLTVQAMIEDAENYEINDFSNWVELLKDYREKEPLRYIDTDDLIKPQWPI
                     QRVGKILGDNAIISTDVGQHQMWTSQFFPFSYPRQWITSGGLGTMGFGLPAALGAARA
                     FKGTNRVVVNFTGDGSILMNIQELMTCSEYELPVINI"
BASE COUNT          411 a          145 c          221 g          336 t
ORIGIN      
        1 gtaataattt cggggcaagt tccaacaaca attattggaa cggatggttt tcaagaaata
       61 gatgctgttg gaatttcaag accatgtaca aagcataact atttggtaaa taaaatagaa
      121 gatttaccaa gaataataaa agaggcattt catctagcaa gtactggaag accaggacct
      181 gttcatgttg atattccaaa agatattaca gcacaaattg gagaatttat ctatcctagt
      241 gaagtaaata tgccaacata taaacctaca ttaaactaca ataaaaagca gttaaaaagg
      301 gctatggaag ctatttcaaa tgctaaaaaa cctcttttat atattggagg aggagctatt
      361 ttatcaaatt gtggatatga aattagagat ttagcaaaaa aactaaatat tccagctgtt
      421 gaaactttaa tggcaagagg agttatgggg gatgaaaatc cacttttctt tggaatgcta
      481 ggaatgcatg gagagtatgc tgcaaatatg gcagctcatg aaacagattt attaatctct
      541 ttaggtgcta gatttgatga tagagttaca ggaaggcttg atgaatttgc atctaaagca
      601 aaagttattc atatagatat tgacccaaca agtattgcaa agcttgtggt tgctgattat
      661 ccaattgttg gagatttaaa acttacagtt caagcaatga ttgaagatgc tgaaaattat
      721 gagataaatg atttctcaaa ttgggttgaa ttacttaaag attatagaga aaaagagcct
      781 ttaagatata ttgatacaga tgatttgata aaacctcaat ggccaattca aagggttgga
      841 aaaatattag gagataatgc aattatttca acagatgttg ggcaacacca aatgtggact
      901 tctcaatttt tcccattttc ataccctaga caatggataa caagtggtgg tttaggaact
      961 atgggatttg gattaccagc agcacttgga gctgctagag cattcaaagg tacaaataga
     1021 gtagttgtta atttcacagg agatggatca attttaatga atattcaaga gcttatgact
     1081 tgtagtgaat atgagttacc tgtaataaat att
//
LOCUS       DAOQAN010000544         1109 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_213069_length_1109_cov_3.850095, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000544 DAOQAN010000000
VERSION     DAOQAN010000544.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_213069_length_1109_cov_3.850095"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="PKO10_07735"
     CDS             <1..231
                     /locus_tag="PKO10_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014473378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNW67074.1"
                     /translation="DIDFIKIDGSLIKNIDQSKDLEIIVKTISNIAKEFDIKTVAEFV
                     ANEHIYNKVKELNIDYSQGYFFDKPLKYEDIV"
     gene            complement(228..851)
                     /gene="gmk"
                     /locus_tag="PKO10_07740"
     CDS             complement(228..851)
                     /gene="gmk"
                     /locus_tag="PKO10_07740"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014473377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HNW67075.1"
                     /translation="MIKELKGAILVISGPSGCGKSTLLKEIYKNIPNYYFSISTTTRA
                     LRGEEEDGVDYHFVSQEEFEKNIKDDKFLEYAKVHNNYYGTMLEPITKALNDGKLVIF
                     DIDVQGHRILRKKLDKLITSVFITTPTLKILEQRLIARDTDSAEMIQKRVQNAKVEIE
                     SFTDYDYLIVNDNIHKASKEILAIANIARAKTKIFDKKSIVENWINS"
     gene            complement(853..>1109)
                     /locus_tag="PKO10_07745"
     CDS             complement(853..>1109)
                     /locus_tag="PKO10_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW67076.1"
                     /translation="NTILKDDKKQINDEADWKDISEIIDEALDEVKDYEFSLDNNKYH
                     LVLNRFKIDELRPLLTKLDQNIIDRLVSGESVDINISIKE"
BASE COUNT          373 a          164 c          117 g          455 t
ORIGIN      
        1 gatatagatt ttataaaaat agatggctct ttaattaaaa atattgacca atcaaaagat
       61 ttagagataa ttgtaaaaac aatatcaaat attgcaaaag aatttgatat aaaaactgtt
      121 gcagaatttg ttgcaaatga gcatatttat aacaaagtaa aagagttaaa tatagattac
      181 tctcaaggat atttttttga taaaccttta aaatatgaag atatagttta actgtttatc
      241 cagttttcaa ctatactttt tttatcaaaa atttttgttt ttgctcttgc tatatttgca
      301 atagctaaaa tctctttaga agctttgtga atattatcat ttacaattaa gtagtcataa
      361 tcagtaaatg attcaatctc tactttagca ttttgaactc ttttttgaat catctcagcg
      421 ctatcagtat ctctagctat taatctttgc tctaatattt ttaaagttgg tgttgtaata
      481 aaaacagaag ttattagctt gtccaatttt tttcttaaaa ttctatgccc ttgaacatct
      541 atatcaaaaa ttacaagttt tccatcattt aaagctttgg taattggttc tagcatagtt
      601 ccataatagt tattatgaac ttttgcatac tccaaaaatt tatcatcttt tatatttttt
      661 tcaaactctt cttgacttac aaaatgataa tcaactccat cttcttcttc acctcttaaa
      721 gctcttgtag ttgttgaaat agagaaatag tagtttggta tatttttata tatctctttt
      781 aaaagagtag attttccgca tccgctaggt cctgaaataa ctagaattgc accttttaac
      841 tcttttatca ttttattcct ttatcgatat atttatatca acagactcac cacttactaa
      901 tctgtctata atattttgat ctagttttgt aagaagtggt cttagctcat caattttaaa
      961 tctattcaaa accaaatgat acttattatt atctaaagag aattcataat ctttaacttc
     1021 atctaaagct tcatctataa tttcacttat atctttccaa tcagcttcgt catttatctg
     1081 ttttttatca tcttttaaaa tagtattaa
//
LOCUS       DAOQAN010000545         1108 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_213427_length_1108_cov_2.953466, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000545 DAOQAN010000000
VERSION     DAOQAN010000545.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_213427_length_1108_cov_2.953466"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="PKO10_07750"
     CDS             complement(<1..405)
                     /locus_tag="PKO10_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNW67077.1"
                     /translation="MIKILLLEDDYLYKISIKEFLEELDFVVDDFDNGEDALNSIFEK
                     RYDLLLLDIRVPKMDGFELVKHVREASIDTPIIILTSLTDIKDLSRGYTLGCNDYIRK
                     PFDMIELKFRIEALIKNHFNSSDDCIELELGFK"
     gene            complement(402..>1108)
                     /locus_tag="PKO10_07755"
     CDS             complement(402..>1108)
                     /locus_tag="PKO10_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HNW67078.1"
                     /translation="KNNIELKKELLKLLNIIETYTKNSIDVQIHTKDLEVFTRSWEDK
                     DFGLKLDSFREGLVKVKNQKEPYVSSELGKRFNIKAIVPIYNDKNIFIGSLEVIVDFK
                     SLVNRLKTLGIDSIVMLEKDYLKIATYHQNNQKFKDYAILESSFTKGLLDILEKNPQF
                     LKKDKFYYELDNRIFTQIPLGEFENSSVGVLFISFDKNINNFRYLPKYDYFIDVEIKD
                     EKNDNKDILKKEIIIR"
BASE COUNT          384 a          181 c           90 g          453 t
ORIGIN      
        1 tttaaatcct aactcaagct caatgcaatc atcacttgag ttaaaatgat tttttataag
       61 tgcctctatt ctaaatttta gctcaatcat atcaaaaggt tttctaatat aatcattgca
      121 accaagagtg tatccacggc ttaaatcttt tatatctgta agtgaagtta aaattataat
      181 tggagtatca atagaggctt ctcttacatg ctttacaagc tcaaatccat ccatctttgg
      241 aactcttata tcaagtaaca gcagatcata ccttttttca aaaattgagt ttaaagcatc
      301 ctctccatta tcaaaatcat caactacaaa atcaagctct tctaaaaact ccttaattga
      361 aattttatat aaatagtcat cttctagcaa caatattttt atcatctaat tattatctct
      421 ttttttaaaa tatctttatt atcatttttt tcatctttta tttcaacatc tataaagtaa
      481 tcatattttg gtaaatatct aaagttgttt atatttttat caaaactaat aaacaaaact
      541 ccaacactac tattttcaaa ctctcctaaa ggaatttgag taaaaattct attatctagt
      601 tcataataaa acttatcttt ttttaaaaat tgtggatttt tttctaaaat atctaataat
      661 ccttttgtaa aactactctc caaaatagca taatctttaa acttttgatt attttgatga
      721 taagttgcaa tttttaaata atctttctct agcataacta tagagtctat ccctaaagtt
      781 ttaagcctat ttacaagaga tttaaaatca actattactt caagagaacc tataaatata
      841 tttttatcat tataaattgg tacaatcgct tttatgttaa atcgttttcc taattcactt
      901 gaaacataag gttccttttg gtttttaacc tttacaagac cctctctaaa actatcaagt
      961 tttaatccaa aatctttatc ttcccaactt cttgtaaata cctctaaatc ttttgtatga
     1021 atttgaacat ctatgctatt tttagtatat gtttctataa tatttaaaag ttttaaaagc
     1081 tcttttttta actctatatt atttttat
//
LOCUS       DAOQAN010000546         1107 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_213793_length_1107_cov_2.647338, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000546 DAOQAN010000000
VERSION     DAOQAN010000546.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_213793_length_1107_cov_2.647338"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1107)
                     /gene="dnaG"
                     /locus_tag="PKO10_07760"
     CDS             complement(<1..>1107)
                     /gene="dnaG"
                     /locus_tag="PKO10_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014469063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HNW67079.1"
                     /translation="EKLSYPETIEKLASMYNYTLEYDTNSVKKSDNKVLEDINAFYQK
                     QFFLDENIQNYVKDRGVFQLSIEKFEIGYASSSNSTIDFLKSNHYNLNDAIEQGVISQ
                     GENGLYARFIDRLIFPIYSINGKLVGFGGRTLTNHGAKYVNSPQTSIFNKSKLLYGYH
                     LAKEKIYKTKEIIVCEGYLDVIMLHQAGFNNAVATLGTALTKDHLPLLRRGEPRVILS
                     YDGDKAGQNAAFKASVMLSQNGFDGGVVIFENGADPADMVKDRKIEELEELFSNPTPF
                     ANFAISYIVSSYNINNPVEKQKALSEANEYLSSLSQLYQEEYKRFLAQKLNIKESLIK
                     TSNTIHRKNSVELSKVDIAELCIIKSILDDPRRL"
BASE COUNT          353 a          195 c          123 g          436 t
ORIGIN      
        1 tccaatcttc taggatcatc caatattgat tttatgatac aaagctctgc aatatcaact
       61 tttgaaagtt ctacactatt ttttctgtgt atagtatttg acgtttttat caaactctct
      121 tttatattta atttttgagc taaaaatctt ttatactctt cttgataaag ttggcttaaa
      181 ctacttaaat attcgtttgc ttcacttaat gctttttgct tctcaacagg attgtttata
      241 ttataacttg aaacaatgta actaattgca aaatttgcaa aaggagttgg atttgaaaat
      301 agctcttcta actcttcaat ttttctatcc tttaccatat cagctggatc cgctccattt
      361 tcaaaaataa caactcctcc atcaaaacca ttttgactaa gcataacaga agccttaaaa
      421 gctgcatttt gtccagcttt atctccatca taagataaaa taactctagg ttctcctctt
      481 cttaaaagtg gtaaatgatc ttttgtaagt gctgttccca aagttgcaac tgcattatta
      541 aacccagctt gatgaagcat tataacatct aagtaacctt cacaaactat tatctctttt
      601 gttttatata tcttctcttt tgctaagtga taaccataca aaagcttgga tttattaaaa
      661 attgaagttt gtggtgagtt tacatatttt gctccatgat ttgtaagagt tcttccccca
      721 aaaccaacaa gttttccatt tattgaataa attggaaata tcaatctatc aataaatctt
      781 gcatataatc cattttctcc ttggctaata actccttgct ctattgcatc gttcagatta
      841 taatgatttg actttaaaaa atctattgtg ctattagaac tacttgcata acctatctca
      901 aatttttcta ttgaaagttg aaaaactcct ctatctttta cataattttg tatattttca
      961 tctaaaaaaa actgcttttg ataaaaagca tttatatctt caagaacttt attatcagat
     1021 ttttttacac tatttgtatc atactccaaa gtgtaattat acattgaagc taatttttca
     1081 atagtttcag gatatgaaag tttctca
//
LOCUS       DAOQAN010000547         1107 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_213819_length_1107_cov_2.444867, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000547 DAOQAN010000000
VERSION     DAOQAN010000547.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_213819_length_1107_cov_2.444867"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..129)
                     /locus_tag="PKO10_07765"
     CDS             complement(<1..129)
                     /locus_tag="PKO10_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004275007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HNW67080.1"
                     /translation="MNKLLWGVIAIIGAACFGYLALQQGETVSAMYLVVAAVCIYMI"
     gene            complement(335..976)
                     /locus_tag="PKO10_07770"
     CDS             complement(335..976)
                     /locus_tag="PKO10_07770"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HNW67081.1"
                     /translation="MNLMLFGAPGAGKGTQAKFLIEKYNIPQISTGDILRAAIAEKTD
                     MGMEAKKFMDEGKLVPDSTIIGIIKDRLVVADCKNGFILDGFPRTLAQAEALSELMQN
                     MKIKLDKVISLNVPDSLIVGRITGRRVCSGCGASFHVEFNPSKKDGVCDYCGGELTIR
                     KDDNAETVKSRLEAYHSQTAPLIDFYKKMGVFMELDGTKDVSEVTKDMINALS"
BASE COUNT          381 a          190 c          129 g          407 t
ORIGIN      
        1 aatcatatag atacaaacag ctgctacaac taaatacata gctgaaacag tttctccttg
       61 ctgtaaagct aaataaccaa agcatgccgc tccaattatt gctattacac cccataagag
      121 tttattcata gcgaatcctt ttttaaataa aatcttttaa gtttactctt aaaaaaataa
      181 aacattagtt aaataattta tataattaat actatttacg actactttat aaatatttac
      241 acaatttata cacaataatt tagaatagga ataaaaatta aaaaagagct atttgctctt
      301 ttttaaaaat agttagtttt gatagaagaa ttatttatga aagagcgttt atcatatctt
      361 ttgtaacttc tgatacatct tttgtaccat caagttccat aaacacaccc atttttttat
      421 aaaaatcaat tagtggtgct gtttgagagt ggtatgcttc aagtctgctt tttacagttt
      481 cggcattatc atcttttcta atagttaatt ctccaccaca atagtcacaa acaccatctt
      541 tttttgaagg attaaactca acatggaaag atgctccaca accagaacaa actcttcttc
      601 ctgtaattct tcctacaatt aagctatcag gaacatttaa agagataact ttatcaagtt
      661 taattttcat attttgcata agttcactca aagcttcagc ttgagccaaa gttcttggga
      721 aaccatcaag aataaatcca tttttacaat cagcaactac aagtctatct ttgataattc
      781 caataattgt tgaatctgga accaatttcc cctcatccat aaatttttta gcttccattc
      841 ccatatctgt tttttctgca attgctgctc ttaagatatc tcctgttgag atttgtggga
      901 tattgtactt ctcaattaga aatttagctt gagttccttt tccagctcct ggtgctccaa
      961 atagcattaa attcatattt tatccttaaa attttttcgc ttattgtagt aaaaaaggat
     1021 ttaatagtag ataagatata aatagtagat taataattta aatgattttt gatacaatga
     1081 ttgcttaaaa ctcaaggctt cattctt
//
LOCUS       DAOQAN010000548         1107 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_213871_length_1107_cov_2.123574, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000548 DAOQAN010000000
VERSION     DAOQAN010000548.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_213871_length_1107_cov_2.123574"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /locus_tag="PKO10_07775"
     CDS             <1..726
                     /locus_tag="PKO10_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNW67082.1"
                     /translation="SLDPYNGITILSEDLPKDILEFEQNLKDLIQNVKDNKNLIWIYI
                     DIKKSDFIPIATKFGFTFHSCNSDYILLVKVLKQNAIVPNLANHTLGVGAVVINSKNE
                     ILLIKEIIRNEYYKLPGGHIDDAEMISQALSREVFEETGVVVDFERIISIGHFYPHQF
                     HKSNLYVLCLAKPKSLKIDVKDKEEISEAIWLDLDEMFKRDDIHDYTKTIVKSALSGQ
                     GLYKSETPILSHLKNEFELFFVK"
     gene            788..1063
                     /locus_tag="PKO10_07780"
     CDS             788..1063
                     /locus_tag="PKO10_07780"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HNW67083.1"
                     /translation="MVAYKRDEIVSVSDISRSFSTILNSIVDKSKEKFAISKNNKLEA
                     VILDIEEYERLKNAYDILEQKEIETLLNSRTKEEKEASHSKIISLDL"
BASE COUNT          451 a          101 c          179 g          376 t
ORIGIN      
        1 agtcttgatc catacaatgg aattacaatt ttaagtgaag atttaccaaa agatattttg
       61 gaatttgaac agaatttaaa agatttaata caaaatgtaa aagataataa aaatttaatt
      121 tggatttata tagatataaa aaaatcagat tttatcccaa ttgctacaaa gtttggtttt
      181 acttttcact cttgcaatag tgattatata ctacttgtga aagttttaaa acaaaatgca
      241 atagttccaa atttggcaaa tcatacttta ggagtaggtg cagttgttat aaatagtaaa
      301 aatgagattt tgctaattaa agagataatt agaaatgagt attataagct tccaggtgga
      361 catattgatg atgctgagat gatatctcaa gctttgagtc gtgaagtttt tgaagaaact
      421 ggtgttgttg tagattttga aagaattata tctataggac atttttatcc acatcaattt
      481 cataaatcaa atctatatgt tttatgtctt gcaaagccaa aaagtttaaa aatagatgta
      541 aaagataaag aggagattag tgaagctatt tggttggatc ttgatgagat gtttaaaaga
      601 gatgatattc atgattacac aaaaactatt gtaaaatcag cattgagtgg tcaaggatta
      661 tataaaagtg aaactccaat tttaagtcat cttaaaaatg agtttgagtt attttttgtt
      721 aagtagtctt gatttaacaa taaatattat gtataatttt acataatatt ttataaagga
      781 aaataaaatg gtagcttata aaagagatga aatagtatct gttagcgata tttcgagaag
      841 tttttcaaca attttaaata gtattgttga taaatcaaaa gagaagtttg ccatctcaaa
      901 aaacaataaa cttgaagctg taattttaga tattgaagag tatgaaagac taaaaaatgc
      961 ttatgatatt ttggaacaaa aagagataga aactttgcta aatagtagaa caaaagagga
     1021 aaaagaagct tcgcattcaa agattattag tttagatttg taagatgaaa aaaaatttca
     1081 aaattaccta tcttaaaaaa tctcaaa
//
LOCUS       DAOQAN010000549         1105 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_214445_length_1105_cov_2.305714, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000549 DAOQAN010000000
VERSION     DAOQAN010000549.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_214445_length_1105_cov_2.305714"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="PKO10_07785"
     CDS             complement(<1..541)
                     /locus_tag="PKO10_07785"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HNW67084.1"
                     /translation="MTQTINTNEVAYKKPQWLRKKLTPHTQVEMENLLKDVGGLHTIC
                     QEAKCPNISECFSNKNATFLILGNICTRRCTYCNVTTGMPKKVDESEIKKVTTSVLTL
                     GLKFVVITSPARDDLVDGGAEQFYKVTRDIIEKSPDTKVEILIPDFKGNDESLKRVIE
                     SGATIIGHNVETVPSLYRIR"
     gene            complement(541..>1105)
                     /locus_tag="PKO10_07790"
     CDS             complement(541..>1105)
                     /locus_tag="PKO10_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014474388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HNW67085.1"
                     /translation="AGQSIDDYKKLQSDYKNNVSKRLTGGGVLFHGHDISYTILLNPN
                     TIENRDVKETYFFICQFLLKFYEDLGLKPKFAKDEESIVLTKSPFCQVGFEAYDIIID
                     GKKIGGNAQKRAKNCILQHGSIPLFTKEDNEVFGSSLQDFGINLNFEEAKDGLIKAFK
                     EVFEVEIFEDSLNEEEKNILEKIEEEK"
BASE COUNT          330 a          202 c          114 g          459 t
ORIGIN      
        1 ttcttatacg atataaagaa ggaactgtct ctacattgtg cccaataatt gtagcacctg
       61 actctataac tctttttaag ctctcatcat ttcctttaaa atcagggatt agtatctcaa
      121 cttttgtatc tggagatttt tcaataatat ctcttgtaac tttataaaac tgctctgctc
      181 caccgtcaac taaatcatct cttgctgggc ttgtaattac tacaaatttt aagcccaaag
      241 ttaaaaccga agtagttact ttttttattt cactctcatc tacttttttt ggcattcctg
      301 ttgttacatt gcaatatgta catcttcttg tacaaatatt tcctaaaatc aaaaatgttg
      361 catttttatt tgaaaaacat tcacttatat ttggacattt tgcctcttgg caaattgtat
      421 gaagtccacc aacatcttta agtaaatttt ccatctcaac ttgtgtatgt ggagttagtt
      481 tttttctcaa ccactgtggc tttttataag ccacttcatt tgtatttatt gtttgtgtca
      541 ctacttttcc tcttctattt tttctaaaat atttttctct tcttcattta aactatcttc
      601 aaatatttca acttcaaaaa cttctttaaa agcttttatt aaaccatctt ttgcctcttc
      661 aaaatttaag tttattccaa aatcttgtaa agaacttcca aaaacttcat tatcttcttt
      721 tgtaaaaagt ggtattgagc catgttgaag aatacaattt ttagctcttt tttgtgcatt
      781 cccacctatt tttttaccat caattataat atcgtatgct tcaaatccaa cttgacaaaa
      841 aggactttta gttaaaacta tactttcttc atcttttgca aattttggtt ttaatcccaa
      901 atcttcataa aattttaata aaaattgaca aataaaaaaa tatgtctctt ttacatcacg
      961 attttcaatt gtatttggat ttaataaaat tgtatatgaa atatcgtgtc catgaaaaag
     1021 tactcctccc ccagttaatc gttttgaaac attatttttg taatcgcttt gtaatttttt
     1081 gtaatcatca atactttgcc ctgct
//
LOCUS       DAOQAN010000550         1104 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_214744_length_1104_cov_2.743565, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000550 DAOQAN010000000
VERSION     DAOQAN010000550.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_214744_length_1104_cov_2.743565"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..763)
                     /locus_tag="PKO10_07795"
     CDS             complement(<1..763)
                     /locus_tag="PKO10_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HNW67086.1"
                     /translation="MNILIIENEVYLAQKVVSRLLDDGHNCDFVENVNLENLTKEYDI
                     VLLSTSISSSIVKGVIKKYGQNSIILLLVSYISDETVTNPIKDGAKDYIMKPFLMDEL
                     VRKIYHYKECRAIRRELKVLKDYFEFTMSDIDIKDVLVPFSFPLLIETNFQSYADKLV
                     FEIAKKVDLPIKFISLSSANWQKQIANQFERTIIYLTDYHTLKRNVKDQLIKQILDKK
                     CVICSLESDDEFTHKKVVFNSKNKSLDHSQIMSIND"
     gene            complement(765..>1104)
                     /gene="ispF"
                     /locus_tag="PKO10_07800"
     CDS             complement(765..>1104)
                     /gene="ispF"
                     /locus_tag="PKO10_07800"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HNW67087.1"
                     /translation="GAIGAGDIGEFFPDTDDKFKGIDSKILLQKIISFVNNVGYELVN
                     IDITIIAQKPKINPHKNEIKSSLSKLLNLPKQFINIKASTAEKLGAVGREEGVVVQAM
                     TNLRYYDWTK"
BASE COUNT          366 a          164 c          122 g          452 t
ORIGIN      
        1 aatcgtttat tgacataatt tgactatgat ctaaagattt atttttacta ttaaaaacta
       61 cttttttgtg agtaaattca tcatcgcttt ctaaagaaca aattacacat tttttatcta
      121 aaatctgttt tattagttga tcttttacat ttctttttaa agtatgataa tcagtaaggt
      181 aaataatagt tctttcaaat tgatttgcta tctgtttttg ccaatttgct gaagataagg
      241 agataaattt tattggtaaa tctacttttt ttgcgatttc aaaaacaagt ttatcagcat
      301 aactttgaaa atttgtctct attagtaagg ggaacgaaaa gggaactaat acatctttta
      361 tatcaatatc actcatagta aattcgaaat aatcttttaa tactttaagc tctcttctta
      421 tagctctaca ctctttatag tgataaatct ttctaactaa ttcatccatt aaaaatggct
      481 tcataatata atcttttgct ccatctttaa taggatttgt tacagtttca tctgaaatat
      541 aagatactaa aagaagtatt attgaatttt gaccatattt tttaataaca ccttttacta
      601 tagaacttga gattgatgtt gaaagaagta caatatcata ctcttttgtt aaattttcga
      661 gatttacatt ttctacaaaa tcacagttat gaccatcatc aagaagtctt gaaacaactt
      721 tctgtgctag ataaacttca ttttcaatta ttagtatatt cattttattt agtccaatcg
      781 taatatctta aatttgtcat agcttgtaca actactcctt cttctcttcc aacagcacct
      841 aacttttcag ctgttgatgc tttgatattt ataaactgct ttggaagatt taaaagtttt
      901 gataaactac tttttatctc atttttatga ggatttattt ttggtttttg agcaattatt
      961 gtaatatcaa tgtttacaag ctcatatcca acattattta caaaacttat aattttttgt
     1021 aaaagtattt ttgagtcgat tcctttaaat ttatcatcag tatctggaaa aaactctcca
     1081 atatcaccag caccaatagc acct
//
LOCUS       DAOQAN010000551         1104 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_214871_length_1104_cov_1.817922, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000551 DAOQAN010000000
VERSION     DAOQAN010000551.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_214871_length_1104_cov_1.817922"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..721
                     /locus_tag="PKO10_07805"
     CDS             <1..721
                     /locus_tag="PKO10_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HNW67088.1"
                     /translation="KLIFDHNYRALEIENPSYADFLSIVKPTKLPPIKNFKTKEGKKY
                     LVHTILHIEYSAVDLALDAALRYQNMPFEFYRDWLEVANDEIRHFLMLEKLLKDLNGF
                     YGEFEVHKNLFEAMQQTPDLLSRMACIPRYLEANGLDQNPKIMEKLNSNKDEFNVKLI
                     EALKIILEEEISHVKKGDFWFKYECDKVGCNPEVAYFDAIEKVFPGSTKRKMDLNFNA
                     RKEAGFSCDELKFLSKKDDCI"
     gene            793..981
                     /locus_tag="PKO10_07810"
     CDS             793..981
                     /locus_tag="PKO10_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW67089.1"
                     /translation="MIDVVIYSVFILALIAFSLSPAIYLTNKLSNKFIFIENNSTKIS
                     ILFAILFSSIATFFIFWF"
BASE COUNT          424 a          109 c          162 g          409 t
ORIGIN      
        1 taagcttatt tttgatcata attacagagc tttagagata gaaaatccat cttatgctga
       61 ttttttatct atagtaaaac caaccaaact accgcctata aaaaacttta aaacgaaaga
      121 gggtaaaaaa tatttagtac acactatttt gcatattgag tatagtgctg ttgatttagc
      181 tttagatgct gctttaagat accaaaatat gccttttgaa ttttatagag attggctaga
      241 ggttgctaat gatgaaatta gacatttttt aatgttagaa aaattattaa aagatttaaa
      301 tggtttttat ggagagtttg aagttcataa aaatctattt gaagctatgc aacaaactcc
      361 cgatttatta agccgtatgg cttgtattcc tagatattta gaagcaaatg gtcttgatca
      421 aaatccaaaa attatggaaa aattaaactc aaataaagat gaatttaatg ttaagttaat
      481 agaggcttta aaaataattc tagaagaaga gataagtcat gttaaaaaag gtgatttttg
      541 gtttaaatat gagtgtgaca aagtagggtg caatccagaa gttgcttatt ttgatgctat
      601 agagaaggtt tttccaggta gcactaaaag aaaaatggat ttaaatttta atgctagaaa
      661 agaggctgga ttttcttgtg atgaattaaa atttttatca aaaaaagatg attgtattta
      721 acttgatata aattaataga ttatattttt aattttgtta tattctaaat gatatttcaa
      781 taaaggatat ttatgataga tgtggttatc tactctgttt ttattttagc actaattgca
      841 ttttcacttt cacctgctat ttatctaaca aataaattgt caaataagtt tatttttata
      901 gaaaataact ctacaaaaat atcaatactt tttgcaattt tattttcaag tattgcaaca
      961 ttttttatat tttggttttg acaaatgata agctcaaact acgatatttt actttttaaa
     1021 acaaatgtaa ataataattt ggaaaaaaat atagataata tattttcaaa tagtgttgaa
     1081 gacatattaa ataaagataa aaga
//
LOCUS       DAOQAN010000552         1101 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_215738_length_1101_cov_2.476099, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000552 DAOQAN010000000
VERSION     DAOQAN010000552.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_215738_length_1101_cov_2.476099"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="PKO10_07815"
     CDS             complement(<1..678)
                     /locus_tag="PKO10_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrodipicolinate N-succinyltransferase
                     N-terminal domain-containing protein"
                     /protein_id="HNW67090.1"
                     /translation="MINTKEDFKALVESIEKESWYKQPLGFGIARVSRGVLNPKLILE
                     ATFPVVNWNENHKSAAVFLAALKQSGQNVDCTKSEAVFDMGDGFLGYCIEAFKPFYEE
                     KELHKNLQVISTLATLPIDSGLSADDFKVVFIFKDEKPQSVEATYLKLYAKYSKKIDE
                     PNLDGIEDLLTNCAWIDAIPVELDWLRQNEILLKVGAKYPKVDYVGKYSRFLRHIIPS
                     NDETSCKA"
     gene            complement(717..>1101)
                     /locus_tag="PKO10_07820"
     CDS             complement(717..>1101)
                     /locus_tag="PKO10_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012013056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HNW67091.1"
                     /translation="NGFGEVVIETKNEEFFKYYLEESNYKEFLKSELEFRKDLYPPFV
                     KLARVVLSSTNGLKIKEELNEIVKDLKNNKDIEVVGFGECAIFKIANKYRYEVIIRSK
                     EVKAILNALHYLKSSNISIDMDTIY"
BASE COUNT          355 a          201 c          102 g          443 t
ORIGIN      
        1 tgccttgcaa gaagtttcat catttgatgg aataatatgt cttaaaaatc ttgaatattt
       61 acctacataa tcaacttttg gatattttgc tccaactttt agcaaaatct cattttgtct
      121 taaccaatca agctctacag gaatagcatc tatccatgca caatttgtaa gtaaatcttc
      181 tattccatca agatttggct catctatttt tttgctatat tttgcataca attttaaata
      241 tgtagcttca acactttgtg gtttttcatc tttgaaaata aatacaactt taaaatcatc
      301 agcacttagt ccactatcta ttggtaatgt tgctaatgta gaaattactt gaagattttt
      361 atgaagctct ttttcttcgt aaaatggttt aaatgcttct atacaatatc ctaaaaatcc
      421 atcacccata tcaaatacag cttcactttt tgtacaatca acattttgcc cactttgttt
      481 taaagctgct aaaaatactg cagcactttt gtgattctca ttccagttta caactggaaa
      541 tgtagcttct aaaattaatt ttgggtttaa aactcctctg ctaactctag caatcccaaa
      601 tcccaaaggt tgtttgtacc aactctcttt ttcgatactt tcaacaagtg ccttaaaatc
      661 ttcttttgta tttatcatat tgctctccta attttaatat taattttact caaattctaa
      721 taaattgtat ccatatcaat agaaatatta cttgatttta aatagtgcaa agcatttaaa
      781 attgctttaa cttcttttga gcgaataata acctcatatc tatatttatt tgcaatttta
      841 aaaattgcac actcaccaaa tcctacaact tcaatatctt tattgttttt taaatctttt
      901 acaatctcat ttaattcttc ttttattttt aaaccatttg ttgaagatag aactactctt
      961 gctaatttaa caaaaggtgg atacaaatct tttctaaatt ctaactcact ttttaaaaac
     1021 tctttataat ttgactcttc taaataatat ttaaaaaact cttcattttt tgtctcaatt
     1081 acaacttctc caaagccatt t
//
LOCUS       DAOQAN010000553         1095 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_217816_length_1095_cov_1.892308, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000553 DAOQAN010000000
VERSION     DAOQAN010000553.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_217816_length_1095_cov_1.892308"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /locus_tag="PKO10_07825"
     CDS             complement(<1..686)
                     /locus_tag="PKO10_07825"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009495209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNW67092.1"
                     /translation="MQIDFANLKIQHNLYKEEIEEAILKVARDCNFIMGSQIDELERN
                     LEKFTGSKYAISCSSGTDALLLAMMALDIQPDDEIITTPFTFFATAETIAFLKAKPVF
                     VDIDEKTYNIDPKKIEEKITPKTKAIIPVSLYGQPADMDEINQIAKKYNLKVIVDGAQ
                     SFGSTYKGISDSNLGDISCTSFFPAKPLGCYGDGGAVFTNDENLYKAMKEIREHGSES
                     RYYHTRLGING"
     gene            complement(750..>1095)
                     /locus_tag="PKO10_07830"
     CDS             complement(750..>1095)
                     /locus_tag="PKO10_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW67093.1"
                     /translation="GIIVSFAAPKLMDTKDSALVSTLKRDVNTAINSIQSYYLLNQKI
                     EDIKDAINIGDTNWDIEKLKMSDKNSCIKLEIKKNQNIVSIDLQVDSAKDSTICKKIR
                     DSGLVSKVYEVY"
BASE COUNT          339 a          180 c          158 g          418 t
ORIGIN      
        1 ccattgatac ccaagcgagt gtggtagtag cggctttcag agccgtgttc gcgaatctct
       61 ttcatagctt tataaagatt ttcgtcgtta gtgaaaacag ctccgccatc accataacaa
      121 cctagaggtt ttgcagggaa gaaagaggta caagatatat ccccaaggtt tgagtcgctt
      181 atacctttat aagtagatcc aaagctttga gctccatcaa caattacttt aaggttatat
      241 tttttagcta tttgatttat ttcatccata tcagcaggtt gtccataaag agatacagga
      301 ataatagctt tagtttttgg tgtaatcttt tcttctattt tttttgggtc aatattataa
      361 gttttttcat caatatcaac gaaaactggt ttagctttaa gaaaagctat tgtttctgct
      421 gttgcaaaga atgtaaaagg agttgtaata atttcatcat ctggttgtat atctaaagcc
      481 atcatagcaa gtaaaagagc atctgttcca cttgagcaag atattgcgta ttttgaacct
      541 gtaaacttct ctaggtttct ttctagttca tctatttgag aacccattat aaagttacaa
      601 tctcgtgcta cttttaaaat agcctcttct atctcttctt tataaagatt gtgttggatt
      661 tttaagttgg caaaatctat ttgcatttgt ttccttaagt aaaaaaataa cgcgaattgt
      721 acttaaaaat cacttaatat ttgttatgct tagtaaactt cataaacctt tgaaaccaat
      781 ccactatctc ttattttttt acaaatagta ctatcttttg cactatctac ttgaagatca
      841 atagaaacaa tattttgatt ttttttaatt tccaatttga tacaagagtt tttatcactc
      901 atttttagtt tttctatatc ccaatttgta tcaccaatat ttatagcatc tttaatatct
      961 tcaattttct gatttaataa atagtaactt tgaatagagt ttatagcagt atttacatct
     1021 cttttcaaag ttgaaacaag agcactatct tttgtatcca taagttttgg agcagcaaaa
     1081 gagacaatta tccct
//
LOCUS       DAOQAN010000554         1094 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_218066_length_1094_cov_2.121270, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000554 DAOQAN010000000
VERSION     DAOQAN010000554.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_218066_length_1094_cov_2.121270"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="PKO10_07835"
     CDS             <1..385
                     /locus_tag="PKO10_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HNW67094.1"
                     /translation="IYPNFYFEKVVVHTLAIGYFVTVLIGFGTRVVLGHSGRKIETKL
                     FALFIFISVQILAVVRIFASISVNFGLNYIFFIELSAILLILALIIWSFKYVAILLEN
                     DKKPEIVVQKVDENKKEEFKPKWKV"
     gene            complement(428..>1094)
                     /locus_tag="PKO10_07840"
     CDS             complement(428..>1094)
                     /locus_tag="PKO10_07840"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNW67095.1"
                     /translation="MFKIILGSLLVATSLLSADLQSSGVEVTLENGKKTTIKREAKPK
                     ECETVAFDPKDIFGGKHEAAPTVDENCKRSFVTYFGKIAPIKASDKIETYGELEVLEF
                     IEKSKTDKNLLLIDSRTENWYNHETIATAINVPYVYTKKSQYPDEFAEALETFGVVEK
                     NGKYDFTNAKTLLMFCNGIWCGQSPEAMKELIAIGYPQEKMKWYRGGMQSWLSVGLST
                     IKP"
BASE COUNT          338 a          172 c          154 g          430 t
ORIGIN      
        1 tatttatcca aatttctatt ttgaaaaagt tgtcgttcat actttagcta ttggatattt
       61 tgttacagtt ttaattggtt ttggtactag agtagttctt ggtcactctg gaagaaaaat
      121 agagacaaaa cttttcgcac tttttatatt tataagtgtt cagattttag ctgtagttag
      181 aatatttgca tctataagtg taaattttgg attaaactat atctttttta tagagctaag
      241 tgctattttg ttgatattag cactaattat ttggtctttt aaatatgttg ctattttatt
      301 agaaaatgat aaaaaaccag aaatagtagt tcaaaaagta gatgaaaata aaaaagagga
      361 atttaagcca aaatggaaag tataaatatt ttagttttca aggcttttgc cttgaaaaca
      421 atatttttta tggtttaata gttgataatc caacactaag ccacgactgc attccacctc
      481 tgtaccattt cattttttct tgaggataac caatagctat taactctttc atagcttcag
      541 gagattgtcc acaccaaatt ccattacaaa acatcaaaag agtttttgca tttgtaaagt
      601 catattttcc atttttttca actactccaa aagtctctaa agcctcagca aactcatctg
      661 ggtattgaga tttttttgta taaacatatg gaacatttat cgccgttgca atagtttcat
      721 ggttgtacca gttttcagtt ctgctatcaa ttaaaagaag gtttttatct gtttttgatt
      781 tctctataaa ctctaaaact tctaactccc cataagtctc tattttatct gatgctttga
      841 ttggtgcaat ttttccaaaa tatgttacaa agcttctttt gcaattttca tctacagttg
      901 gagctgcttc gtgtttccca ccaaaaatat cttttgggtc aaatgctaca gtttcacact
      961 cctttggttt agcttccctt tttatagttg tttttttacc attttctaat gttacttcaa
     1021 ctccagaact ttgtaaatcg gcacttagta gagaagtggc tacaagtaaa cttcctaaaa
     1081 ttattttaaa cata
//
LOCUS       DAOQAN010000555         1093 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_218284_length_1093_cov_2.827553, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000555 DAOQAN010000000
VERSION     DAOQAN010000555.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1093
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_218284_length_1093_cov_2.827553"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            18..611
                     /locus_tag="PKO10_07845"
     CDS             18..611
                     /locus_tag="PKO10_07845"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNW67096.1"
                     /translation="MRYDVIIIGAVAAGFGCALTLGSANDKFDWAKDKKYLILDDNKS
                     DLNVGKYFNVAGVDSGINGVDLLLKMKKQLEIYKDVELKDEKATKIENLGEYFKITTE
                     NSTYEASIVVLATGLHKFDIECEAVNVKDNIFVPKPNNIYLENSNNLISKNLYVAGLA
                     SGVPTMFSCASGDGAKVACDIFKLWSGKIAVVHDVKA"
     gene            complement(641..>1093)
                     /locus_tag="PKO10_07850"
     CDS             complement(641..>1093)
                     /locus_tag="PKO10_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002949647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-4 component G"
                     /protein_id="HNW67097.1"
                     /translation="ILFEMNAKAMAAGNSVGQAGLNASKDQQSVLDFLSGKGKINDMS
                     LEDIGYKGKPIMDLSPQEATDLVGENGFFGITQTSDRVSNFVFSFAGDDLEKLQKGRD
                     GIVKGFEEANKMFGGNLPEISYKTQERTLALIDAKIESLKNPQSKVEE"
BASE COUNT          376 a          153 c          170 g          394 t
ORIGIN      
        1 aatgaaagga aaatattatg agatatgatg taataattat tggtgctgta gctgctggtt
       61 ttggttgtgc tttaacttta ggaagtgcaa atgataaatt tgattgggca aaagataaaa
      121 aatacttaat tttagatgat aataaaagtg atttaaatgt tggaaaatat ttcaatgttg
      181 ctggagttga tagtggaatc aatggagttg atttacttct taaaatgaaa aaacagctag
      241 aaatatataa agatgttgaa ctaaaagatg aaaaagctac taaaattgag aatttaggcg
      301 aatattttaa aataacaact gaaaattcta cttatgaagc ttcaattgta gtattagcaa
      361 ctggtcttca taaatttgat atagagtgtg aagctgttaa tgtaaaagat aatatttttg
      421 ttccaaaacc aaataatatc tatttggaaa attcaaacaa tttaatctct aaaaatctat
      481 atgtagctgg acttgcaagt ggtgttccta caatgttttc atgtgcaagt ggagatggag
      541 ctaaagttgc ttgtgatatt tttaaacttt ggagtggaaa aatagcagtt gttcacgatg
      601 taaaagcata aaaatagaag cttttgcttc tatttttata ttactcttct acttttgatt
      661 gtggattctt caaagattca atttttgcat ctattagagc taaagttctc tcttgagttt
      721 tgtatgaaat ttctggtaaa tttccaccaa acattttatt tgcttcctca aaacctttaa
      781 caattccatc tcttcctttt tgaagttttt ctaaatcatc tccagcaaaa ctaaaaacaa
      841 agtttgaaac tctatctgaa gtttgagtta ttccaaaaaa accattttca cctacaagat
      901 ctgttgcctc ttgtggcgat aaatccataa ttggttttcc tttatagcca atatcctcaa
      961 gactcatatc atttatttta cctttgccgc ttaaaaaatc caaaacactt tgttgatctt
     1021 ttgaagcatt tagacctgct tgtcctactg aattaccagc agccatagct tttgcattca
     1081 tctcaaataa aat
//
LOCUS       DAOQAN010000556         1089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_219606_length_1089_cov_2.872340, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000556 DAOQAN010000000
VERSION     DAOQAN010000556.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_219606_length_1089_cov_2.872340"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..138)
                     /locus_tag="PKO10_07855"
     CDS             complement(<1..138)
                     /locus_tag="PKO10_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HNW67098.1"
                     /translation="MLTVMTGPCVLEDMDTVLKIAEKLRPLSLDKRVDFYFKASFDKA
                     NR"
     gene            complement(286..>1089)
                     /locus_tag="PKO10_07860"
     CDS             complement(286..>1089)
                     /locus_tag="PKO10_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNW67099.1"
                     /translation="AKELSDKLSTIEIVFFRNVFGVFFILFSIYKSPLKQLGGKFWLL
                     IFRGVAGFSALLFFFYNIANIPLGEAMTFSKTSTIFTALLAYFFLKEQIGIKGWIGVI
                     VGFIGILFIAEFDGSSLEKTDYLGILSGVGAALAYTSIRELRRFYDSRAIVLSFMAVG
                     TIFPLIFMIVSEFYTNPHLDFMLGTFVMPEARDWIFIALLGVFSTYAQIYMTKSYSLA
                     KAGIVSTVSYSNIAFSIVLGLFMGDAFPSFIVLLGIILIVISGIMVSKK"
BASE COUNT          422 a          200 c          122 g          345 t
ORIGIN      
        1 tctgtttgct ttgtcaaaac tagctttaaa ataaaaatca actcttttat caagactaag
       61 aggtcttaat ttttctgcta tttttaaaac tgtatccata tcttctaaaa cacaaggtcc
      121 tgtcataact gtcaacataa tatattttcc ttttatttaa ttggttccca attaaataaa
      181 aggaaccata ctatattaaa ctacttttta acggaattta taaaaaattc ctaaaagtag
      241 caaaaactct aaagggcttc gagaacgaag ccttgaacta atttattatt tcttactaac
      301 cattattcca ctaattacaa tcaaaattat acccaaaagc actataaaag atggaaaagc
      361 atcacccata aaaagcccca aaactattga aaaggctata ttactataag aaaccgtgct
      421 tacaattcca gcttttgcaa gagagtatga ttttgtcata taaatttgtg cataagtaga
      481 aaaaactcct aataaagcta taaaaatcca atctcttgcc tctggcatta caaaagttcc
      541 tagcataaaa tcaagatgtg ggtttgtgta aaactcactt actatcataa aaatcaaagg
      601 aaaaattgta ccaactgcca taaaagataa aactatggct ctgctgtcat aaaatcttct
      661 aagttctcgt attgaagtat atgcaagtgc tgctccaaca cctgataaaa ttcctaaata
      721 atctgttttt tctaagctac ttccatcaaa ctcggctata aataaaattc caataaatcc
      781 tacaattact ccaatccaac ctttaattcc aatttgctct tttaagaaaa aatatgccaa
      841 aagtgctgta aatattgtag aagtttttga aaaagtcatt gcctcaccca aaggaatatt
      901 tgctatatta taaaagaaaa atagtagtgc cgaaaaccca gcaactcctc taaaaataag
      961 taaccaaaat tttccaccta gctgttttag cggacttttg tatatagaaa atagtataaa
     1021 aaatactcca aaaacatttc taaaaaacac tatttcaata gtgcttaatt tatcactaag
     1081 ttcttttgc
//
LOCUS       DAOQAN010000557         1088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_220033_length_1088_cov_2.184898, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000557 DAOQAN010000000
VERSION     DAOQAN010000557.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_220033_length_1088_cov_2.184898"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1078)
                     /locus_tag="PKO10_07865"
     CDS             complement(<1..1078)
                     /locus_tag="PKO10_07865"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HNW67100.1"
                     /translation="MLDAFSPQSLFCVGDYDQSIYAFNGSDIGIISSFSKRYPDANVF
                     TLRKNYRSTKPILDLATKVIEHNERIYPKKLEVVREQNIHLPKLLAFDELFSQYEYIS
                     ELISKSSTEHSEIAIIYRNNSSADGIEANLREFGIPAKRKGGMSFFDSVEVKFILDIL
                     VLQVISNDMMAFIHIVEHGKGIGKAIAKDIFDALIKLGSGNLLNGLFYPNKDINNPYE
                     TKRKSQQLGLFDDFIELGTVSKFKDCNFEEAFLGNPILKHPKLGVDGAKYIYDLYLLL
                     KQLKRTKNPETMVTSISSSMIYSKLKDMLSTKRATQKDGTVNSIQKTKSLAKINRKAM
                     LLKNLSRNFSDLSKFINSMILGGSE"
BASE COUNT          359 a          171 c          127 g          431 t
ORIGIN      
        1 gctcactacc acctaaaatc attgagttta taaattttga taaatcagaa aaattccttg
       61 ataaattttt aagcaacata gcttttctat ttatttttgc caaagatttt gttttttgta
      121 tagaatttac tgtaccatct ttttgtgttg ctctttttgt tgataacata tcttttaatt
      181 tagagtaaat cattgaagag ctaattgatg taaccattgt ttctggattt tttgttcttt
      241 ttaattgttt taaaagaaga tataaatcat aaatatattt agctccatca actcctagtt
      301 ttggatgttt taatattgga tttcctaaaa atgcttcttc aaaattacaa tctttaaatt
      361 tagaaacagt tcctagttct ataaaatcat caaaaagacc tagttgttgg ctttttcttt
      421 ttgtttcata aggattattt atatctttat ttgggtagaa aagcccattt aataaattcc
      481 cactcccaag ttttattaaa gcatcaaaaa tgtcttttgc aatggctttt cctatccctt
      541 ttccatgttc aactatatga ataaaagcca tcatatcgtt gcttataact tgtaaaacca
      601 aaatatctaa aataaattta acttctacag aatcaaaaaa gctcattcca ccttttcttt
      661 ttgcaggaat tccaaactct cttaaatttg cctcaatccc atctgcacta gagttatttc
      721 tataaattat tgcaatttca ctgtgttctg tactactttt tgagataagt tctgaaatat
      781 attcatactg tgaaaaaagc tcatcaaaag ctagaagttt tggaagatga atattttgtt
      841 ctctaacaac ttctaatttt tttggataaa ttctttcatt gtgctcaata acttttgttg
      901 ctaaatctaa aataggtttt gtagatctat aattttttct taaagtaaaa acatttgcat
      961 ctggatatct tttactaaaa ctagagataa taccaatatc agaaccattg aaagcataaa
     1021 tgctttgatc ataatctcct acacaaaata gtgattgtgg agaaaaagca tcaagcaacc
     1081 ttccttgt
//
LOCUS       DAOQAN010000558         1086 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_220595_length_1086_cov_2.555771, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000558 DAOQAN010000000
VERSION     DAOQAN010000558.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1086
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_220595_length_1086_cov_2.555771"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..912)
                     /locus_tag="PKO10_07870"
     CDS             complement(<1..912)
                     /locus_tag="PKO10_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014467923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdH/YhfP family quinone oxidoreductase"
                     /protein_id="HNW67101.1"
                     /translation="MKAFVVEKNHNDEIISGVKDVLKPICGENEVIIKATYSSLNYKD
                     ALSSVGNPGVTRVFPHITGIDVAGEIVETKSNIFKVGQKVIVTGYDLGMNTNGGHSQY
                     VKVPASWVVAIPSSLSDKEAMIYGTAGLTAALSINELISNNITQESGDILVTGATGGV
                     GSLAVAILNKLGFNVVALSGKEDKISYLKEIGASEVVLREEFLKDSSKALLKPRFAGV
                     IDTTGGDILSTALKQTNYDGVVTCCGLTASSSLNTNIFPFILRGVRLIGIDSVECILD
                     KKQDAWEKLAGKYSIKNLNEITNEISLD"
BASE COUNT          371 a          218 c          113 g          384 t
ORIGIN      
        1 atccaagctt atttcatttg taatttcatt taagttttta atactatatt tacctgctaa
       61 tttttcccaa gcatcttgtt ttttatctaa aatacactca acactatcaa tcccaattaa
      121 tcttacacct cttagaataa atggaaaaat atttgtattt aaactacttg aagctgtaag
      181 tccacaacaa gtaacaactc catcataatt tgtctgtttt aaagctgttg ataaaatatc
      241 tccacctgtt gtatcaatta ctcctgcaaa tcttggtttt aaaagagctt ttgagctatc
      301 ttttaaaaac tcttctctta aaaccacttc actcgcacca atttctttca aatagctaat
      361 tttatcttcc tttccagaaa gagctactac attaaaacct aatttattca aaattgcaac
      421 tgccaaagag ccaactccac ctgttgcacc agtaactaaa atatcaccac tttcctgtgt
      481 tatattatta cttataagtt catttatact caatgctgct gtaagtcctg ctgttccata
      541 aatcatagcc tctttatcgc ttaaagagct aggaattgct accacccaag aagcaggaac
      601 tttaacatat tgactatgcc caccatttgt attcatacct aaatcatatc cagtaacaat
      661 aactttttgc cctactttaa agatattaga ttttgtctca acaatctctc ctgcaacatc
      721 aatgccagta atatgtggaa aaactctagt aactccagga tttccaactg aactaagagc
      781 atctttgtaa tttaaagatg aatatgtagc cttaataata acttcatttt caccacaaat
      841 tggtttaagc acatctttta caccagaaat aatttcatca ttatgatttt tttcaactac
      901 aaaagctttc atcttcttcc ttttaacttt tttatttaaa actttagcta aaatgtttta
      961 caatagcaag aactatcttt taagaaatgt acttactaaa aagatacttt tggagtgaaa
     1021 tatgactaaa agaataaacc aagataatat aaatactgag tttaaatgcc ctgtagaaac
     1081 ggctct
//
LOCUS       DAOQAN010000559         1085 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_220884_length_1085_cov_2.777670, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000559 DAOQAN010000000
VERSION     DAOQAN010000559.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_220884_length_1085_cov_2.777670"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="PKO10_07875"
     CDS             complement(<1..524)
                     /locus_tag="PKO10_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HNW67102.1"
                     /translation="MSKFFKIIDLIVGTINQTIAVYGMVLGVLLAFVNVVLRYVFDMS
                     LPWAAELTNYLFIWSALFGAAYGFKQGAHISITLLIEKFSPSVMKMFLLFANLLSIIY
                     LLLISYFGYKLILMLMDFGEINVDLQVPLWIPQLVIPIAFFLAAYRVAEKLVELYKTD
                     AQNIKLFSEHEAII"
     gene            complement(538..>1085)
                     /locus_tag="PKO10_07880"
     CDS             complement(538..>1085)
                     /locus_tag="PKO10_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DctP family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HNW67103.1"
                     /translation="SSKKAIINPEDAKGQKFRIQSSKVLEAQFKAVGGNPQILPFSEV
                     YSALQQGVVDATENPLSNFYTKKFNEVQSSLTLSSHGYLGYLVVMNQQFWDKLPKDLQ
                     DKVSQAMKEATEFERKATAEDDAKIMADLKKYAAESKKLEIFELNDAQKAEWRKVMEG
                     IYPQFYDVIGEDLIKKAIDTK"
BASE COUNT          380 a          184 c          133 g          388 t
ORIGIN      
        1 tctattatag cttcatgttc actaaatagt ttgatattct gagcatctgt tttataaagt
       61 tctacaagtt tttcagcaac tctatatgca gctaaaaaaa atgcaatagg aataactaat
      121 tgaggtatcc aaagtggaac ttgtaaatct acgttgattt ccccaaaatc cattaacatc
      181 aaaattaatt tatatccaaa atatgaaatc agaagaagat aaataataga aagtaaattt
      241 gcaaaaagca aaaacatttt cataacagaa ggtgaaaact tctctattaa caaagtaata
      301 gagatgtgag caccttgctt aaacccatag gcagcaccaa aaagtgccga ccatataaat
      361 aaatagtttg taagttcagc tgcccaagga agactcatat caaagacata tcttagaaca
      421 acattaacaa atgccaataa aacacccaat accattccat agactgctat tgtttgattt
      481 attgtaccaa ctattaaatc aataatttta aaaaatttac tcatttttcc gctttactta
      541 ttttgtatca attgcttttt tgattaaatc ttcaccaatt acatcataaa attgtggata
      601 aataccttcc ataacttttc tccattcagc tttttgtgca tcatttaatt caaaaatctc
      661 aagttttttt gactcagctg catatttttt taaatcagcc ataatttttg catcatcttc
      721 tgcagttgct tttctttcaa actctgttgc ttctttcata gcttgtgata ctttatcttg
      781 taaatctttt ggtaatttat cccagaattg ttgattcatt acaactaaat aacctaaata
      841 tccatggcta cttaaagtaa gactagattg aacttcatta aatttttttg tataaaaatt
      901 tgatagagga ttttcagttg catcaacaac accttgttga agtgctgaat aaacttcaga
      961 gaatggtaaa atttgtggat ttccaccaac agctttaaac tgagcttcta aaacttttga
     1021 agattgaatt ctaaattttt gacctttagc atcttcagga tttataatag cttttttgct
     1081 acttg
//
LOCUS       DAOQAN010000560         1083 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_221585_length_1083_cov_2.709144, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000560 DAOQAN010000000
VERSION     DAOQAN010000560.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1083
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_221585_length_1083_cov_2.709144"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            175..642
                     /locus_tag="PKO10_07885"
     CDS             175..642
                     /locus_tag="PKO10_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW67104.1"
                     /translation="MEISNNTNLILNSNLNTNSSVSEEQKDRFDIFLENQEKDKAEKK
                     ERSELLGDLEFVSKTGFTKEELERIKELLEELQKQRAESGQAPLSPEEYIQALKQDLQ
                     TAIYEVTGKKVDLDNETIQNIMNLQKSGGNETFVKPTTDEELRLVDEFKKASK"
     gene            658..>1083
                     /locus_tag="PKO10_07890"
     CDS             658..>1083
                     /locus_tag="PKO10_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014475049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNW67105.1"
                     /translation="MRLDLYLSKNFDIQSRNKALELILSNKVKVDGKIVSKASFLVDE
                     KMKIELLEEDFYVSRAAYKLKYFLDELKNKNEINLKDKIALDIGSSTGGFTQILLEFD
                     IQKVVCVDVGSNQLHEKIKKDNKIEFFENCDIRDFKSDIS"
BASE COUNT          487 a           99 c          182 g          315 t
ORIGIN      
        1 aacatatatt gcaaatgcta cgtatagaat agtaataaaa gttgaagaca atattactaa
       61 aattgtagaa gttgttgctg ttgggaaaag agagcataaa gagatttata aagaagctta
      121 taaaagaata agcataaaca aatctaaata aaaaattaag aaaagaggga ttttatggaa
      181 atttcaaata atacaaattt aattttaaat tcaaatctaa atacaaatag ttcagtaagt
      241 gaagaacaaa aagataggtt tgatattttt ttagaaaatc aagaaaaaga taaagctgaa
      301 aaaaaagaga ggagtgaact tttaggtgac ttagagtttg tttctaaaac tggatttaca
      361 aaagaagagt tagagagaat taaagagctt ttagaagagt tacaaaaaca aagagccgaa
      421 tcaggacaag cacctcttag ccctgaagag tatattcaag ctttaaaaca agatttacaa
      481 actgctattt atgaagttac aggtaaaaaa gtagatttag ataatgaaac tattcaaaat
      541 ataatgaatt tacaaaaaag tggtggtaat gaaacttttg taaaacctac aacagacgaa
      601 gagctaagac ttgtagatga atttaaaaaa gcatctaaat aagatactta taaatatatg
      661 agattagatt tatacttaag caaaaacttt gatattcaaa gtagaaataa agcacttgaa
      721 ctaattcttt caaataaagt aaaagttgat ggcaaaattg tatcaaaagc ctcattttta
      781 gttgatgaaa agatgaaaat agaacttctt gaagaggatt tttatgtaag tagagctgca
      841 tacaaactaa aatatttttt agacgagcta aaaaataaaa atgagattaa cttaaaagat
      901 aaaatagctc ttgatattgg aagtagcaca ggtggtttta cacaaatttt attagaattt
      961 gatattcaaa aagtagtttg tgttgatgtt ggaagcaatc aacttcatga aaagattaaa
     1021 aaagataaca aaatagagtt ttttgaaaac tgcgatatta gagattttaa aagtgatatt
     1081 agc
//
LOCUS       DAOQAN010000561         1082 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_221849_length_1082_cov_3.001947, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000561 DAOQAN010000000
VERSION     DAOQAN010000561.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_221849_length_1082_cov_3.001947"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(30..752)
                     /gene="kdsB"
                     /locus_tag="PKO10_07895"
     CDS             complement(30..752)
                     /gene="kdsB"
                     /locus_tag="PKO10_07895"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014474308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HNW67106.1"
                     /translation="MIIIPARLNSSRFANKILVDIFGLPMVIRTAKQVSSLDKVVIAT
                     DTKEVYDLAKEHGFEAVMTSVEHNSGTDRINEAVNSLNLSDDEIIVNVQADEPFIEID
                     VVKAVINRVKEVKDCQNTLITSCYKEINSQSADDPNLVKVVINNLEEAIYFSRAKIPY
                     HRDGDENSTYFGHLGIYGFTKKSLNKFCSLNSSKLENIEKLEQLRAIDNGFKIAMVKV
                     NSKSFGIDTKEDLERALKIFSK"
     gene            complement(811..>1082)
                     /locus_tag="PKO10_07900"
     CDS             complement(811..>1082)
                     /locus_tag="PKO10_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW67107.1"
                     /translation="QHGQYWSEKIQIPIVAKTTSGKIVAGFCKYSDNKVKKSEFNKFL
                     EDLKSEDISADIIVIFSKNGCSNELKNLKSDSLRLFNTKSLKAII"
BASE COUNT          330 a          172 c           97 g          483 t
ORIGIN      
        1 taatttttaa taaaatcttc tttttttagt tacttactaa atatttttaa agctctttct
       61 aaatcctctt ttgtatctat tccaaaagat tttgaattta cttttaccat agcaatttta
      121 aacccattat caatagctct taactgttct agtttttcta tattttcaag ttttgaagag
      181 tttaaactac aaaatttatt tagagatttt tttgtaaatc catagattcc taaatgtcca
      241 aaataagtag aattttcatc accatctcta tgatatggaa tttttgctct tgaaaaatat
      301 attgcttctt caagattatt tattacaact tttactaaat ttggatcatc tgcactttga
      361 ctatttatct ctttgtagca agaagttatt aaagtatttt gacaatcttt tacctctttt
      421 actctattta ttacagcttt tacaacatca atttcaataa aaggttcatc tgcttgaaca
      481 tttacaataa tttcatcatc acttaggttt agactattta cagcttcatt tattctatct
      541 gttccactgt tatgttcaac acttgtcata actgcttcaa agccatgctc ttttgctaaa
      601 tcataaacct cttttgtatc tgttgcaatt actactttat ccaatgaact aacctgttta
      661 gcagttctta taaccatagg aagtccaaaa atatctacta aaattttatt tgcaaatcta
      721 cttgaattta atcttgctgg aattattatc ataacagttt ctctttcatt atttttttat
      781 tttattatat agttttatat ctttaaaaat ttatataatt gcttttaaac tttttgtatt
      841 aaatagtctt agtgaatcac tttttaggtt ttttagctca tttgaacagc cattttttga
      901 aaatataaca ataatatcag cagatatatc ttcacttttt aaatcttcta aaaatttatt
      961 aaattcactc ttttttactt tattgtcact atatttacaa aatcctgcaa ctatttttcc
     1021 tgaagttgtt tttgctacta ttggaatttg gattttttca ctccaatact gtccatgttg
     1081 tt
//
LOCUS       DAOQAN010000562         1081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_222186_length_1081_cov_2.795322, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000562 DAOQAN010000000
VERSION     DAOQAN010000562.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_222186_length_1081_cov_2.795322"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="PKO10_07905"
     CDS             <1..508
                     /locus_tag="PKO10_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HNW67108.1"
                     /translation="EPIFAEFEKMIKKIEEVSQKVTTIEQDAIIKGKEFDTQVIKAIK
                     DLKQCATFFEQAAFGFESKLLKTSISIAQKIINIEVGENSSKIAKQTINQLLLKLKNA
                     TKVKIHLNPKDYYILKQELELEPFIELLEDPNVVAGGVVIASNIGNFDGSIEAKVSSM
                     LESLDLVI"
     gene            557..844
                     /locus_tag="PKO10_07910"
     CDS             557..844
                     /locus_tag="PKO10_07910"
                     /inference="COORDINATES: protein motif:HMM:NF013236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HNW67109.1"
                     /translation="MEISERVYDLLVDTEIVVDVMLGSTNISVGEFLKLTEGDIISLH
                     KPAGSGGEIYVNSRIIGTGDIIVMEEKLAVRVQDAMDSDNVVQYFFEEHMI"
     gene            866..>1081
                     /locus_tag="PKO10_07915"
     CDS             866..>1081
                     /locus_tag="PKO10_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HNW67110.1"
                     /translation="MAEVNSANSVTGTDSFGNKYTQSVSNDSLTTNDFLKLMIEELKL
                     QDPTKPMDSSKMLSTQMQMSTLNANMEM"
BASE COUNT          431 a          118 c          192 g          340 t
ORIGIN      
        1 tgagcctatt tttgctgaat ttgagaaaat gataaaaaaa attgaagaag tgtcacaaaa
       61 agttacaact attgaacaag atgcaataat caaaggcaaa gagtttgata ctcaagttat
      121 aaaagcaata aaagatttaa agcagtgtgc aacatttttt gaacaagctg cttttggttt
      181 tgagagtaaa ttattaaaaa cttctatctc tattgctcaa aaaattataa atattgaagt
      241 aggagaaaac tcttctaaaa tagctaaaca gactataaat cagcttttat taaaactaaa
      301 aaatgcaaca aaagtaaaaa ttcatttaaa tccaaaagat tattatattt taaaacaaga
      361 gttggaatta gaacctttta ttgagctttt agaggatcca aatgtagttg ctggtggtgt
      421 tgtaatagct agtaatatag gaaattttga tggaagtatt gaagcaaaag tctcttcaat
      481 gcttgaatct ttagatttag tgatataaaa tctaatttat gatacaatca ttaaataaca
      541 ttaaaaagta ttaaatatgg aaataagtga aagagtttac gatttattag tcgataccga
      601 aattgttgtt gatgttatgc ttggaagcac taatatatct gttggtgaat tcttaaaact
      661 aacagaagga gatattatct ctttgcacaa accagctggt agtggtggag agatttatgt
      721 aaactctaga attattggaa caggtgatat aattgtaatg gaagaaaaac ttgcagttag
      781 agttcaagat gctatggatt cagataatgt tgttcaatat ttctttgaag aacatatgat
      841 ttaattttaa acaaggagtt taagtatggc agaagtaaat agtgcaaata gtgtaacagg
      901 aacagatagt tttgggaaca aatatacaca aagtgtaagt aacgactctt tgacaacaaa
      961 tgatttttta aagctaatga ttgaggagtt gaagcttcaa gatccaacaa aaccgatgga
     1021 ttcatcaaaa atgctatcta ctcaaatgca aatgagtact ttaaatgcaa acatggagat
     1081 g
//
LOCUS       DAOQAN010000563         1079 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_222840_length_1079_cov_2.692383, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000563 DAOQAN010000000
VERSION     DAOQAN010000563.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1079
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_222840_length_1079_cov_2.692383"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>267)
                     /locus_tag="PKO10_07920"
     CDS             complement(<1..>267)
                     /locus_tag="PKO10_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014474282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HNW67111.1"
                     /translation="LEMLKDTIDFFTSNGYEMVGMDHFAKPEDELFKAIKKGELHRNF
                     QGYTTKGGADLIGIGVTSIGNGVDYYAQNFKDLNEWEEAIDNGNL"
     assembly_gap    268..277
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<278..1058)
                     /gene="hemN"
                     /locus_tag="PKO10_07925"
     CDS             complement(<278..1058)
                     /gene="hemN"
                     /locus_tag="PKO10_07925"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013135464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HNW67112.1"
                     /translation="MIDYEKFVKYSKPGPRYTSYPTAPEFSENFKEEDLKDFYKKQDK
                     NRALSLYIHLPFCRSACYFCGCNTIFTSKEDKKTRYIEYLKKELNILKNHLDTSRVVT
                     QMHFGGGTPTYFSPQQLEDVIKSIKTIFPNFSSDAEISCEVDPRYFTKEHMDVLKAGG
                     FNRLSFGVQDLDETVQKTIHRIQPFELTLNVINIARDAGIKSINTDLIYGLPHQTKES
                     FKKTLEKMLTLNVDRFAVFNYAHVPWLMKTMRKFDETTFPTP"
BASE COUNT          333 a          174 c          132 g          430 t
ORIGIN      
        1 agatttccat tatcaatagc ttcttcccat tcgtttaaat ctttaaaatt ttgtgcataa
       61 taatcaacac catttcctat agacgtaaca ccaattccta ttaaatctgc tccacctttt
      121 gttgtataac cttggaaatt tctatgtaat tcaccttttt ttatcgcttt aaaaagttca
      181 tcttctggtt ttgcaaagtg atccattcca accatttcgt agccatttga tgtaaaaaag
      241 tctattgtat cttttagcat ctcaagtnnn nnnnnnnttg gagttggaaa ggttgtttca
      301 tcaaattttc tcatagtttt cataagccaa ggaacatgtg catagttgaa tactgcaaat
      361 ctatctacat ttagtgttag cattttctct aaagtttttt tgaaactctc ttttgtttgg
      421 tgaggcaaac catagattaa atctgtattt atagatttaa taccagcatc tcttgctata
      481 tttattacat ttaaagttag ttcaaaaggt tgaattctgt gaatcgtttt ttgaacagtt
      541 tcatctaaat cttgaactcc aaaactaaga cgattaaaac caccagcttt taatacatcc
      601 atatgctctt ttgtaaagta tctaggatca acttcgcaag atatttcagc atcactgcta
      661 aaatttggaa aaatagtttt tatagatttt ataacatctt ctagttgttg cggtgaaaaa
      721 tatgtaggag ttcctccacc aaagtgcatt tgtgttacaa ctctagatgt atctaagtga
      781 ttttttaata tatttaactc tttttttaaa tattcaatat atcttgtttt tttatcttct
      841 tttgaagtaa aaatagtatt acaaccacaa aaataacatg cacttctaca aaaaggaaga
      901 tgaatatata aagacaaagc tctattttta tcttgtttct tataaaaatc ttttaaatct
      961 tcttctttaa aattttcact aaactcaggt gctgttggat atgaagtata tctaggacct
     1021 ggttttgagt attttacaaa tttttcataa tctatcatct atttctcatc tcttttttt
//
LOCUS       DAOQAN010000564         1079 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_222871_length_1079_cov_2.436523, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000564 DAOQAN010000000
VERSION     DAOQAN010000564.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1079
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_222871_length_1079_cov_2.436523"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..878
                     /locus_tag="PKO10_07930"
     CDS             <1..878
                     /locus_tag="PKO10_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNW67113.1"
                     /translation="VQAIVNVGDVAVVPDPTYPIHSYAFMLNGAAIHKFELVFDNEFK
                     VDEELFFKNLQKTIDESIPKVKYVVVNFPHNPSCATVTPEFYTKLVEMAKRERFYIIS
                     DIAYADITFDGYKTPSIFQAEGALDVAVECFTLSKSYNMAGWRVGFIVGNEKLVGALK
                     RIKSWLDYGMFAPIQIAATVALDGPQDCVDEIVSKYEKRRDVMLQAFAEAGWVMDKPN
                     ASMFIWAKIPEKARHLGSLEFSKQLLREAQVAVSPGIGFGHYGDQYVRIALIENEKRI
                     RQAAKNIKRYLKTLE"
     gene            891..>1079
                     /locus_tag="PKO10_07935"
     CDS             891..>1079
                     /locus_tag="PKO10_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HNW67114.1"
                     /translation="MLKVGIIGVGTVGTSVVNILRDNKNIITARAGVEIVPTIGVVSN
                     LNKKRDVQIELTDDVSKVL"
BASE COUNT          387 a          126 c          213 g          353 t
ORIGIN      
        1 tagttcaagc tattgtaaat gttggagatg tagcagttgt tccagatcct acttatccaa
       61 ttcactcata cgcatttatg ctaaatggtg cagctattca taaatttgag ttagtatttg
      121 ataatgagtt taaagtagat gaagagttgt tttttaaaaa tcttcaaaaa acaattgatg
      181 agtcaattcc aaaagtaaaa tatgtagttg taaacttccc tcacaatcca agttgtgcaa
      241 ctgtaactcc agagttttat acaaaacttg tagagatggc aaaacgagag agattttata
      301 taatctctga tattgcttat gctgatatta catttgatgg atataaaact ccttcaattt
      361 ttcaagcaga gggtgcttta gatgttgctg ttgagtgctt tacattaagt aaatcatata
      421 atatggctgg atggagagtt ggttttattg ttggaaatga aaaacttgta ggtgctttaa
      481 aaagaataaa atcatggctt gattatggaa tgttcgcacc tattcaaatt gctgcaactg
      541 ttgcacttga tggtccacaa gattgtgtag atgagatagt ttcaaaatat gaaaaaagaa
      601 gagatgttat gcttcaagca tttgctgaag ctggttgggt tatggataaa ccaaatgcat
      661 ctatgtttat ttgggcaaaa atacctgaaa aagctagaca tttaggaagt ttagagtttt
      721 ctaaacaact tttaagagaa gctcaagttg cagttagtcc tggaattggt tttggacatt
      781 atggtgacca gtatgtaaga attgctttga ttgaaaatga aaaaagaatt agacaagctg
      841 caaaaaatat aaaaagatat ttaaaaacat tagaatagga aaaaataacc atgcttaaag
      901 ttggaattat tggagtagga acagttggaa caagtgttgt aaatatttta cgagacaata
      961 aaaatattat aactgctcga gctggagttg aaattgttcc tactattgga gtagtttcaa
     1021 atttaaataa aaaaagagat gtgcaaatag agcttactga tgatgtttca aaagtttta
//
LOCUS       DAOQAN010000565         1078 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_223197_length_1078_cov_2.525904, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000565 DAOQAN010000000
VERSION     DAOQAN010000565.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_223197_length_1078_cov_2.525904"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(214..>1078)
                     /locus_tag="PKO10_07940"
     CDS             complement(214..>1078)
                     /locus_tag="PKO10_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="imelysin family protein"
                     /protein_id="HNW67115.1"
                     /translation="KNVALKDTQSAINSAKKLQKELNDENFTKFLKDWKRVEANYLAG
                     EINSDYLDTPRYIDVFNNLKEDLNSQMQRVIDGNLDIKKALFKNSFKTINALEYLLYS
                     SKELNDRKKEIGREILNSIIKNLEDIKTVYETYLKNPIIKDDDNAKLINTLVASSYRL
                     KEWRIGNPAGFSTKYKNDAKNSRAEYFLSQNSFEAIDSILDAQKEMIANEGYINLLNL
                     AKDKNATNELEAVIAKIDEAKKELKSLPKDDFTNAKKLFDLTSQIHDLYYITIIDKLG
                     LKAEILDADGD"
BASE COUNT          345 a          156 c          123 g          454 t
ORIGIN      
        1 cctgtgtaat tatactaaat ttgctaaaaa aaagtaaata taatcaagtt ttgcactagg
       61 atgtaaccac aattttgaag ataatataac tctactattt ttttttgaaa aatagtagta
      121 gataaatgcc aaaaaaattg aagtcagcaa atagagccaa tagactcttt tgtttggatt
      181 tgttaaaaaa tcaaaggcaa aatagtccaa ggtttaatct ccatctgcat ctaaaatttc
      241 agctttaagt cccaatttat caataatcgt aatatagtat aaatcatgaa tttgtgaagt
      301 caaatcaaaa agtttttttg catttgtaaa atcatcttta ggaagagatt ttaactcttt
      361 ttttgcttca tctatttttg caataacagc ttctagctca tttgttgcat ttttatcttt
      421 tgctaaatta agaagattta tataaccttc atttgcaatc atctcttttt gagcatctaa
      481 aattgaatct atagcttcaa aagagttttg gcttaagaaa tactcagctc tactattttt
      541 tgcatcattt ttatattttg ttgaaaatcc tgctggattt ccaattctcc actcttttag
      601 tctataagaa gatgcaacaa gtgtatttat aagttttgca ttgtcatcat cttttattat
      661 tggatttttt agataagttt cataaacagt ttttatatct tcaagatttt ttataattga
      721 gtttaaaatc tctcttccta tctctttttt tctatcattt aactcttttg aagagtaaag
      781 cagatattcc aaagcattta tagttttaaa tgagttttta aatagtgctt tttttatatc
      841 caaattgcca tctataactc tttgcatttg agaatttaaa tcctctttta aattgttaaa
      901 aacatcaata tatcttggag tatctaaata atcgctattt atttccccag caagatagtt
      961 tgcttcaact cttttccaat cttttaaaaa ctttgtaaag ttttcatcat ttaactcttt
     1021 ttgtaatttt tttgcactat ttatagcact ttgtgtatct tttagtgcta catttttt
//
LOCUS       DAOQAN010000566         1077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_223496_length_1077_cov_2.878669, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000566 DAOQAN010000000
VERSION     DAOQAN010000566.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_223496_length_1077_cov_2.878669"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="PKO10_07945"
     CDS             complement(<1..294)
                     /locus_tag="PKO10_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014474681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate- and nitrite sensing domain-containing
                     protein"
                     /protein_id="HNW67116.1"
                     /translation="MISKLSIKQKLIAIMLIPLVIVILLAAKLAYDSYRSLENLKSLD
                     KVVILSTKIGALVHETQKERGMTAGFIGSKGVKFKDELPRQREEVNKQITFLNE"
     gene            423..>1077
                     /locus_tag="PKO10_07950"
     CDS             423..>1077
                     /locus_tag="PKO10_07950"
                     /inference="COORDINATES: protein motif:HMM:NF012304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase-like protein"
                     /protein_id="HNW67117.1"
                     /translation="MKIDKEFLNKIYYNGDLNKKQLDILNIDFSSEKNYENKILGQEI
                     SKNDSNLLMLLRGIDDLKSQEQIVSNYHLVLEHNNIKANVYKNEDNNISTSNNILKIY
                     CDGACSGNPGNAGSGIAIYFDDKNPVLLYGDFIESGTNNIAELNALYKALQIADESKI
                     NSTISIFTDSKYAIDCISTWAYSWKKNGWSKKGGEIKNLPLIIKSHELFEKLKSKINL
                     "
BASE COUNT          429 a          136 c          142 g          370 t
ORIGIN      
        1 ttcatttaaa aaagttattt gtttgtttac ctcttctctt tgtcttggta gctcatcttt
       61 aaacttaaca cctttacttc caataaatcc agctgtcata cctctctctt tttgagtttc
      121 atgaaccagt gcaccaattt ttgtagataa aataacaact ttatctaatg atttaaggtt
      181 ttccaagctt ctgtatgagt cataagctaa ttttgctgct agcaaaatta caataaccaa
      241 tggaataagc ataattgcaa ttagcttttg ttttatagat agttttgaaa tcattcataa
      301 atcctaattt tttgtgtaac tctaccataa atttggctat ttttggttaa tttacaacaa
      361 caataaagcc ctatttttgt acaatcttca attgaaaaaa attaattata agtaaaagta
      421 atatgaaaat agacaaagaa tttttaaaca aaatttatta caatggtgat ttaaataaaa
      481 aacagcttga tatattaaat atagatttta gctctgaaaa aaattatgaa aataaaattt
      541 taggacaaga aatttcaaaa aatgactcaa atttactaat gcttctaaga ggcattgatg
      601 atttaaaatc tcaagagcaa atagtttcaa actaccattt agtattagaa cacaacaata
      661 taaaagcaaa tgtatataaa aatgaagata acaatatctc aacatcaaat aatattttaa
      721 aaatatattg tgatggagct tgtagcggaa atcctggaaa tgctggaagt ggaatagcca
      781 tttattttga tgacaaaaat ccagttttat tatatggtga ttttattgag agtggaacaa
      841 acaatattgc tgaattaaat gctttataca aagctttaca aatagcggat gaatcaaaaa
      901 ttaatagtac tatatctatt tttacagact caaaatatgc aatagattgt atatcaacat
      961 gggcatattc atggaaaaaa aatggttgga gtaaaaaagg tggagagata aaaaatcttc
     1021 ctttgataat aaaatctcat gagctttttg aaaagttaaa atcaaaaata aacttag
//
LOCUS       DAOQAN010000567         1075 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_224249_length_1075_cov_2.396078, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000567 DAOQAN010000000
VERSION     DAOQAN010000567.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_224249_length_1075_cov_2.396078"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1057)
                     /locus_tag="PKO10_07955"
     CDS             complement(<1..1057)
                     /locus_tag="PKO10_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014474740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNW67118.1"
                     /translation="MKKEINESINEPVVYLAKERKSNKISPVWILPLIILGILAFIAY
                     DTYSKKGTNIVVYFKSAEGLKENVTTLEYKGLALGKVTKISMHDDLKNVAVNILVNSD
                     VAEYVANEGSKFWIKKPTVSLTKISGLSTLISGYKIELSPNFKIENAKPQWYFTGLDS
                     APDDEFEENGYYISLLMNDKDNVDVGTPIFYNKYQIGEIVSKEFKNEQLYASAYIYDK
                     YNYLVNKSSKFIMNEALKVNYGASGLNIEVSSLYSALVGGITVVTSIKDDSKIEKNEI
                     YTLFSSKDDLRKKVHFTIDFNEGKIDENTSILYKGIEIGKVSNVKIEDDIIATKAYIY
                     EEYKYLLTKNSRFIIEEP"
BASE COUNT          353 a          175 c          104 g          443 t
ORIGIN      
        1 ttggttcttc aataataaaa cgactatttt ttgtaagaag atatttgtat tcttcataaa
       61 tataagcttt tgtagctatt atatcatctt ctatttttac atttgatact ttacctattt
      121 caataccttt gtatagtatt gaagtatttt catctatttt tccttcatta aaatcaatag
      181 taaaatgaac tttttttctt aaatcatctt ttgaagaaaa aagagtataa atctcatttt
      241 tttcaatttt cgaatcatct tttatacttg ttacaactgt aattccacca actaaggctg
      301 aatatagaga gctaacttca atatttaaac cactagcacc ataatttact tttaatgcct
      361 cattcattat aaattttgaa cttttattta ctaagtagtt atacttatca taaatatatg
      421 cacttgcata aagttgttca tttttaaact cttttgaaac aatttcacct atttggtatt
      481 tgttataaaa aattggagtt ccaacatcta cattatcttt atcattcatt aataaagata
      541 tataataacc attctcttca aattcatcat ctggagcaga atcaagacct gtaaaatacc
      601 attgtggttt tgcattttct attttaaaat ttggagataa ctctatttta taaccgctta
      661 ttagtgtgct aagtcctgaa atttttgtca aagatacagt tggttttttt atccaaaatt
      721 ttgaaccttc atttgcaaca tattctgcaa catcactatt tactaaaata ttaacagcaa
      781 cattttttaa atcatcatgc atagatattt ttgtaacttt tcctaaggct aaacctttgt
      841 actccaaagt tgtaacattc tcttttaaac cttcagcact tttgaaatat acaacaatat
      901 ttgtcccttt tttagagtat gtatcataag caataaatgc aagaattccc aaaattataa
      961 gtggaagtat ccaaacagga gatattttat tactttttct ctcttttgct aaatacacaa
     1021 ctggctcatt tatactttcg ttaatctctt ttttcatccc aaattatcct tgtat
//
LOCUS       DAOQAN010000568         1075 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_224302_length_1075_cov_2.089216, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000568 DAOQAN010000000
VERSION     DAOQAN010000568.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_224302_length_1075_cov_2.089216"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /locus_tag="PKO10_07960"
     CDS             complement(<1..665)
                     /locus_tag="PKO10_07960"
                     /inference="COORDINATES: protein motif:HMM:NF019884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HNW67119.1"
                     /translation="MEHDKLATRLSLIIYKLNQGERLTIESLANEFGVSKRTIERDIA
                     RFSYFDIKKEGKEFFLDELAVGKLNFDDIKNFAIFSGIKSLYPSLTNQFLKDILNEKI
                     NRAYMVQNSGFEDIQEKQQLFENLSTAIIEEKTISFFYNDKKRVSFFKDSCAKVVETQ
                     CIKELFFLDSINANGSISKDIYILKKKNLLWTVWLMKKNRVIFDDLSLRKLLNDTIFY
                     FNST"
     gene            complement(691..>1075)
                     /gene="folE"
                     /locus_tag="PKO10_07965"
     CDS             complement(691..>1075)
                     /gene="folE"
                     /locus_tag="PKO10_07965"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HNW67120.1"
                     /translation="EMVVVKDIEFYSFCEHHMLPIIGKAHVAYIPNGKVVGLSKIPRV
                     VDVFARRLQIQEQLTEQICDALNEHLKPKGVAVIVDARHMCMEMRGVQKICSTTITSS
                     LRGLFKSDKKTKDEFLSIVSASFQK"
BASE COUNT          357 a          180 c          118 g          420 t
ORIGIN      
        1 gttgaattaa aataaaatat ggtgtcgtta agtaattttc ttagtgataa atcatcgaaa
       61 atgactctgt tttttttcat taaccaaact gtccacaata agtttttttt cttcaaaata
      121 taaatatctt ttgaaataga tccatttgca ttaattgaat ctaaaaaaaa taattctttt
      181 atgcattgag tctcgacaac cttagcacat gagtccttaa aaaaactcac tcttttttta
      241 tcattgtaaa aaaagctaat agttttttct tctattattg cagtgcttaa gttttcaaat
      301 agttgttgtt tttcttgtat atcttcaaag ccactatttt gaaccatata tgctctattt
      361 attttttcat ttagaatatc ttttaaaaac tgatttgtta aagatggata caaagattta
      421 ataccactaa aaattgcgaa gtttttaata tcatcaaagt ttagttttcc aacagcaagt
      481 tcatccaaaa agaactcttt gccctctttt tttatatcaa aataggaaaa tctagcaata
      541 tctctttcta ttgttctttt tgatacgcca aactcatttg ctaaagattc aatagttaat
      601 ctctctcctt gatttaattt ataaatgata agagataatc tagtggctaa tttatcgtgt
      661 tccaatttgt gtttccttct atttcaaaat ctacttttga aaagaagctg aaactatact
      721 caaaaactca tcttttgttt ttttatcact tttaaaaagt cctcttaaag aagaagttat
      781 agttgtagaa catatttttt gcactcctcg catctccata cacatatgtc ttgcatctac
      841 aataactgca actccttttg gttttaaatg ctcatttaat gcatcacaaa tttgctcagt
      901 aagttgctct tgaatttgta atcttcttgc aaaaacatcc acaactctag gaatttttga
      961 aagtccaaca acttttccat ttggaatata tgcaacatgt gcttttccaa taattggcaa
     1021 catatgatgc tcacaaaatg agtaaaactc aatatctttt acaacaacca tttca
//
LOCUS       DAOQAN010000569         1071 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_225606_length_1071_cov_2.179134, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000569 DAOQAN010000000
VERSION     DAOQAN010000569.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_225606_length_1071_cov_2.179134"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..>1071
                     /locus_tag="PKO10_07970"
     CDS             98..>1071
                     /locus_tag="PKO10_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012013118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNW67121.1"
                     /translation="MNKYILPLYNRLDIAFIKGKKSLLFDENKKDYIDFASGVGVNSL
                     GYANKKVAKIIEKQAKKILHSSNIYRIKPQEECAKKIVELSTYDMQCFFCNSGAEANE
                     TAIKLARKYGNVTFKTPKYKIITIKNSFHGRTIATLKATAQEDKHKYFAPYPDGFVYA
                     NDINEAICMIDDTTVGVMLELIQGEGGIFMQDIFQVQRLERILKEKKLLLIIDEVQTG
                     VYRSGNFLISQYFGIKPDIVTLAKGLASGIPIGVMMTSLKDIFTFGDHGSTFGGNHLS
                     TTVCKAVLKILEKYSNSGKLSENIKYFNSCLQYFTDKYPNIFLQKNGWG"
BASE COUNT          432 a          104 c          184 g          351 t
ORIGIN      
        1 tttggggcat caatttaaaa actctttaac aaaaagatat gagctaggag atgaggataa
       61 tcaatttagc ttttatcata cataaaaaag gaaagttatg aataaatata tattaccttt
      121 atataatcga ttagatattg catttataaa aggtaaaaaa tctctattat ttgatgagaa
      181 taaaaaagat tatatagatt ttgcttcagg agttggagta aatagtttag gttatgcaaa
      241 taaaaaagta gcaaaaataa tagaaaaaca agcaaaaaaa attttacaca gttcaaatat
      301 atatcgaata aaaccgcaag aagagtgtgc taaaaaaata gttgaattaa gcacttatga
      361 tatgcagtgt tttttttgta atagtggagc agaagcaaat gaaacagcta taaaacttgc
      421 tagaaaatat ggaaatgtga catttaaaac acctaaatat aagattatta ctataaaaaa
      481 ctcttttcat ggaagaacaa tagcaacttt aaaagcaaca gcacaagaag ataaacataa
      541 atattttgct ccatatcctg atggatttgt ttatgcaaat gatataaatg aagctatttg
      601 catgattgat gatacaactg ttggtgttat gttagagttg attcaaggtg agggtggtat
      661 ttttatgcaa gatatttttc aagtacaaag acttgaaaga attttaaaag agaagaagtt
      721 acttttgata atagatgaag ttcaaacagg agtttataga agtgggaatt ttttgatttc
      781 tcaatatttt ggaataaaac ctgatattgt aactttagca aaaggtttag caagtggtat
      841 tccaataggt gttatgatga caagtttgaa agatatcttt acttttggtg atcatggttc
      901 aacttttggt ggaaatcatc tttcaacaac agtttgtaag gctgttctaa agattttaga
      961 gaaatattca aatagtggaa aacttagtga aaatataaaa tattttaaca gttgtttaca
     1021 atattttaca gataaatatc caaatatctt tttacaaaaa aatggttggg g
//
LOCUS       DAOQAN010000570         1069 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_226245_length_1069_cov_2.318540, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000570 DAOQAN010000000
VERSION     DAOQAN010000570.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_226245_length_1069_cov_2.318540"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="PKO10_07975"
     CDS             complement(<1..379)
                     /locus_tag="PKO10_07975"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNW67122.1"
                     /translation="MKKVVFTSLIFASYLFGTSIENIVQKSLQNNFDIKSLENSIEIA
                     NFQIKQAKNWENPMISFKANDIMLKKNYLNNQKEYGIELSQAIPIGKKLELEESIAKK
                     DKLLKEQTLQDIKLEFESKIYLYS"
     gene            complement(381..764)
                     /locus_tag="PKO10_07980"
     CDS             complement(381..764)
                     /locus_tag="PKO10_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012011972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HNW67123.1"
                     /translation="MKKVLKAFVVMLLMATILNASSLKESLNVDGYNLELTSKRDLSA
                     GSNEFFVKITKDGKEVNDAKIKAKFFMPEMPGMPYMEHEGEGKFENGIYSFVINFCMD
                     GTWQYNIRFKTADDKVHSVKSSVSF"
     gene            complement(839..>1069)
                     /locus_tag="PKO10_07985"
     CDS             complement(839..>1069)
                     /locus_tag="PKO10_07985"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNW67124.1"
                     /translation="NIDIILQNRELIIKDSGIGISKDKIKDIFNRYYRATNQSGGFGL
                     GLNIVNMICKTYNIKIDVQSLENIGSTFTLKL"
BASE COUNT          331 a          171 c          109 g          458 t
ORIGIN      
        1 atgaatatag ataaattttt gattcaaatt ctagctttat atcctgtaga gtttgttctt
       61 tcaataattt atcttttttt gcaatactct cttcaagttc aagtttttta ccaataggta
      121 tggcttgaga taactcaatt ccatactctt tttgattatt taaatagttt ttcttcaaca
      181 taatatcatt tgctttaaag cttatcattg gattttccca atttttagcc tgttttattt
      241 ggaaattagc aatttcaata gagttttcaa ggcttttaat atcaaaatta ttttgtaaac
      301 tcttttgtac tatattttca atcgaagttc caaataaata actagcaaaa attagtgaag
      361 taaaaactac ttttttcatt ttagaagctc acgctacttt ttacagagtg aactttatca
      421 tcagctgttt taaatctgat attatattgc caagttccat ccatacagaa attaattaca
      481 aaactataaa tgccattttc aaattttcct tcaccttcat gctccatata tggcattcct
      541 ggcatctctg gcataaaaaa cttagctttt atttttgcat catttacttc ttttccatct
      601 tttgtgattt tcacaaaaaa ctcattactt cctgcactta aatctctttt gcttgtaagc
      661 tcaagattgt atccatcaac atttaaactc tcttttaatg agcttgcatt taaaatagta
      721 gccattagaa gcataactac aaaagctttt aatacttttt tcattttcat taatccttta
      781 ttttgattta tgaaaattta ccaaaagctt tgtgtagttt gtgtgtagat ttatattttt
      841 atagttttaa agtaaaagtt gaacctatat tttccaaaga ttgaacatca attttgatat
      901 tgtaagtttt gcaaatcata tttacaatat ttagtcctaa accaaagcct ccactttgat
      961 ttgttgctct ataatatctg ttgaaaatat cttttatttt atcttttgat attccaatac
     1021 cgctatcttt tattattaac tctctatttt gcaagataat atctatatt
//
LOCUS       DAOQAN010000571         1068 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_226570_length_1068_cov_2.557749, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000571 DAOQAN010000000
VERSION     DAOQAN010000571.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_226570_length_1068_cov_2.557749"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1068)
                     /locus_tag="PKO10_07990"
     CDS             complement(<1..>1068)
                     /locus_tag="PKO10_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510159.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HNW67125.1"
                     /translation="MEAIREFQLLHSEVGTTLGLSNISFGLAQNARIYLNSIYLDHCV
                     KAGLTTAIVNVKHIIPLNKISPEDKKACDDLIFNNQENGDPLFKFIEHFSNVESQDEQ
                     SDEEYQKMEPIEKVKKLLLDGDKERMIPLVLELKDTIAPELIVNEWLIDGMKIIGELF
                     GSGQMQLPFVLQSAETMKATVDALNPYLPKQEKSTETTVVIGTVKGDVHDVGKNLVDI
                     ILSNNGFKVVNIGIKADLNQFLEAAKEHNADAIGMSGLLVKSTAVMKENLEEMQRLGI
                     KIPVMLGGAALTKNFVDDYCRPIYDGAIFYCRDAFDGVVSMQRIEKGGELDTKLAADL
                     IEKIDTSDRVEKEEAVIPPYE"
BASE COUNT          335 a          205 c          126 g          402 t
ORIGIN      
        1 tcttcataag gaggaattac agcctcctct ttttcaactc tatcacttgt atcaattttt
       61 tcaattaaat cagcagctag ttttgtatca agctctccac ctttttcaat tctttgcata
      121 gatacaacac catcaaaagc atctctacaa taaaaaatag ctccatcata aattggtcta
      181 caataatcat caacaaagtt tttagttaaa gcagcaccac caagcataac tggaattttt
      241 attccaagtc tttgcatctc ttcaagattc tctttcatta cagctgtact ttttacaagt
      301 aatccactca ttccaatagc atcggcgttg tgctcttttg cagcttctaa aaattggttt
      361 aaatcagctt ttattccaat atttactact ttaaaaccat tgttacttaa aataatatca
      421 actaaatttt taccaacatc gtgaacatca cctttaactg taccaattac aacagtagtt
      481 tcagtacttt tttcttgttt tggtaaatat ggatttaaag catcaacagt agctttcata
      541 gtttctgcac tttgaagtac aaatggaagt tgcatttgac cacttccaaa tagctctcca
      601 attattttca ttccatcaat tagccactca tttacaatta attcaggagc tatagtatct
      661 tttagctcta aaactaaagg aatcattctc tctttgtcac catctaataa aagtttttta
      721 accttttcaa taggttccat tttttgatac tcttcatcac tttgttcatc ttgagattca
      781 acatttgaga agtgttctat aaatttaaat agtgggtcac cattttcttg attgttaaat
      841 atcaaatcat cacaagcttt tttatcttca gggcttattt tatttagtgg aataatatgc
      901 ttaacattta caatagcagt tgtaagtcct gcttttacac agtgatctaa atatattgag
      961 tttaaataaa ttcttgcatt ttgagctaag ccaaatgaga tatttgaaag tcctaaagtt
     1021 gttccaactt cgctatgaag aagttggaat tctctaatag cttccata
//
LOCUS       DAOQAN010000572         1068 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_226607_length_1068_cov_2.267522, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000572 DAOQAN010000000
VERSION     DAOQAN010000572.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_226607_length_1068_cov_2.267522"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..927
                     /locus_tag="PKO10_07995"
     CDS             <1..927
                     /locus_tag="PKO10_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HNW67126.1"
                     /translation="IKNFLHHVLKNHFKTYKTPRSVNTIVGLVLDVNRDLPKDTQIYI
                     AEAGARVKGDIKTIANFLEPQIAVVGSVGEQHIEYFKTLENIKNIKKELLLSPRLQKA
                     FVHSSANVVPSEKIEEFPNNLHIVKSNLDGLWFDMELNGKVEHFYAPILGSFNAINLA
                     AVVLVATHLGMSVLEINEALTTLPQVEHRLQKIEANGKIIIDDSFNGNLEGMLEAINI
                     SSNYSGRKVIITPGLVESTDSANILLAKEIDKVFDFVIITGTLNANILKANINEDKVF
                     VLKDKTMLETTLARTTKSGDLILFANDAPNFI"
BASE COUNT          426 a          117 c          189 g          336 t
ORIGIN      
        1 ataaaaaact ttttacacca tgttttaaaa aatcatttta aaacatataa aacaccaaga
       61 agtgtaaata ctattgtagg tcttgttctt gatgtaaata gagatttacc aaaagataca
      121 caaatatata tagcagaagc aggggcaaga gtaaaaggtg atataaaaac aatagcaaac
      181 tttttggagc cacaaattgc tgttgttgga agtgttggag agcaacatat tgagtatttt
      241 aagactttag aaaatattaa aaatataaaa aaagagcttt tactatctcc tagattacaa
      301 aaagcatttg ttcatagtag tgcaaatgta gttcctagtg aaaagataga ggagtttcca
      361 aataatcttc atatagtaaa aagtaatctt gatggtcttt ggtttgacat ggagttaaat
      421 ggaaaagttg aacactttta tgcaccaatt ttaggaagtt ttaatgctat aaacttagca
      481 gcagttgttt tagtagcaac tcatttagga atgagtgttt tagagataaa tgaagcttta
      541 acaactttac cacaagtaga acacagactt caaaaaattg aagcaaacgg aaaaattata
      601 atagatgact cttttaatgg aaatcttgaa ggaatgttag aagctattaa tatctcttca
      661 aactatagtg gaagaaaagt gataatcact ccagggcttg tagaatctac tgatagtgca
      721 aatattcttt tggcaaaaga gatagataaa gtttttgatt ttgtaataat aactggaact
      781 ttaaatgcaa atattttaaa agccaatata aatgaagata aagtttttgt tttaaaagat
      841 aagacaatgc ttgaaacaac tttagcaaga actacgaaaa gtggtgattt aatactgttt
      901 gcaaatgatg caccaaattt tatatagggt aaaaaatgga acatatttat gcaccttggc
      961 gatataacta tgtaaccgat gaaaaggtaa aagggtgtgt tttttgtcat attagtgaaa
     1021 acttagaaga tgaaaaatta caagtgatat ttagtgatga atattgtt
//
LOCUS       DAOQAN010000573         1065 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_227685_length_1065_cov_2.010891, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000573 DAOQAN010000000
VERSION     DAOQAN010000573.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_227685_length_1065_cov_2.010891"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="PKO10_08000"
     CDS             complement(<1..202)
                     /locus_tag="PKO10_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW67127.1"
                     /translation="MMQVIIVISIFLILLTVIIYKINDKFEKKEFLFLVAITIFISTI
                     VIFYEKYQNNYLPNMFKAKYENE"
     gene            524..>1065
                     /locus_tag="PKO10_08005"
     CDS             524..>1065
                     /locus_tag="PKO10_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HNW67128.1"
                     /translation="MTVVGFIYQDFKQMQEIQTKKIVSIQLADELRQSSDDLTRLARL
                     FSVTGDSKYEKMYWDVIKIRNGEIARPEDYHRIYWDLVLEYGQKPKPDGKKVVLLEAL
                     KEAGITQKELALLDEASKNSDKLVGIETTAMNAAKGLFADSNGKYTIKREPDLDYAAK
                     LMHSQEYMNEKAKIVKPIDD"
BASE COUNT          401 a          111 c          173 g          380 t
ORIGIN      
        1 tctcattttc atactttgct ttaaacatat ttggaagata gttattttga tatttttcgt
       61 aaaaaataac aatagttgat ataaaaatag tgatagctac caaaaaaaga aactcttttt
      121 tctcaaattt atcattgatt ttatatataa taaccgtgag taaaattaga aatattgaaa
      181 ttacaataat aacttgcatc aaaatactcc attgtttttc cgcgattata tcaaagcttt
      241 ctaaaattat ttaaaatatc aaaactgtca aaattttggt acataaattt tacataagtt
      301 tttgtttagt tttaatatat taaagtttag gaagaatttt gtttgttact tttaaataat
      361 ttatttaaac taatattatt ttaaaataat atttttgaca ataaatatct gtgatataat
      421 aaagttcgtt attttattaa aggtgggggg gggaatgaat atgtccttta aaagcttctt
      481 tttatacctt aaactgattg gtattttttc agtaattggt attgtgacag ttgttggttt
      541 tatctatcaa gattttaaac aaatgcaaga gattcaaaca aaaaagattg tatctattca
      601 acttgcagat gagttaagac aaagttctga tgatttgact agattagcaa gattgttctc
      661 tgtaactggt gatagcaaat atgaaaaaat gtattgggat gttataaaaa taagaaatgg
      721 tgagatagca agacctgaag attatcacag aatttattgg gatttggtac ttgaatatgg
      781 tcaaaaacca aagccagatg ggaagaaagt tgttttattg gaggctttaa aagaggctgg
      841 gattacacaa aaagagttag ccttattaga tgaagcttca aaaaattctg acaaactagt
      901 tggaattgaa actactgcta tgaatgctgc aaaaggtttg tttgctgatt caaatggtaa
      961 atatactatc aaaagagagc ctgatttaga ttatgcagca aaacttatgc actctcaaga
     1021 gtatatgaat gaaaaggcaa agattgtaaa accaatagat gattt
//
LOCUS       DAOQAN010000574         1064 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_228070_length_1064_cov_1.836472, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000574 DAOQAN010000000
VERSION     DAOQAN010000574.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_228070_length_1064_cov_1.836472"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..802
                     /locus_tag="PKO10_08010"
     CDS             <1..802
                     /locus_tag="PKO10_08010"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNW67129.1"
                     /translation="TSAPVAALFAIMTKVGIYSIIRVHGTIFGQNANELAFYYTPWVL
                     YIGLITLVLATFGVLSSKELKTQISYLVLLSVSIILIAIGINSKEALSGAIFYMIHST
                     FVAGGFFLLADVILKARNSTSLRPKMPIFKNAILIGSIFFIFAIAIAGLPPFSGFFGK
                     IMILKSSINHTQTALIFTTVLLSSLLVIVSLARSGTNIFYDTRAKEEESNYKLSKSTL
                     SSIVYLFSFTIILVLFANNITHITENIASNLVDTNSYISTVLNPNGEL"
     gene            804..>1064
                     /locus_tag="PKO10_08015"
     CDS             804..>1064
                     /locus_tag="PKO10_08015"
                     /inference="COORDINATES: protein motif:HMM:NF014012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HNW67130.1"
                     /translation="MKDTNSFLPHPILSFMLVIIWLLLNNTVAAGHIVLGIVLAILIP
                     WFTSSFWQRKVYIGSFFTLFKFVIIVAYDIIVANLTVARQVMD"
BASE COUNT          350 a          133 c          155 g          426 t
ORIGIN      
        1 aacaagcgct ccagttgctg ctttatttgc tattatgaca aaagttggaa tttattcaat
       61 cataagagtt catggaacta tttttggaca aaatgcaaat gaattagcat tttactacac
      121 tccttgggtt ttatatattg gacttataac tttagtttta gcaacttttg gagttttaag
      181 ttcaaaagag cttaaaactc aaatatctta tttagtttta ttatctgtta gtattatttt
      241 aatagctatt ggaataaatt caaaagaggc attaagtgga gcaatatttt atatgattca
      301 ctcaactttt gttgcaggtg gatttttctt attagctgat gttattttaa aagcaagaaa
      361 tagcacatct ttaagaccca aaatgcctat ttttaaaaat gcaatactaa taggaagtat
      421 attttttata tttgctattg ccatagctgg acttccacct tttagtggct tttttgggaa
      481 aattatgatt ctaaaatctt caataaatca tacacaaaca gctttaatat ttactactgt
      541 tttattaagt agcctattag ttattgtatc tttagcaaga agtgggacaa atatatttta
      601 tgatacaaga gcaaaggaag aagagtcaaa ttataaatta tcaaaatcaa ctctatcatc
      661 tattgtttat ctattttcat ttactattat tttggtactt tttgcaaata atattacaca
      721 cataacagaa aatatagctt caaatttagt tgatacaaat agttatatat cgactgtttt
      781 aaatccaaat ggggagcttt aatatgaaag atacaaatag ttttttgcct catccaattt
      841 tatcttttat gcttgttatt atttggttac tactaaataa tacagtagct gctgggcata
      901 ttgttttggg aattgtatta gcaattttaa taccttggtt tacatcaagt ttttggcaaa
      961 gaaaggttta tataggctct ttttttactc tttttaaatt tgtgattatt gtagcttatg
     1021 atattatagt tgctaattta actgttgcta gacaagttat ggat
//
LOCUS       DAOQAN010000575         1063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_228248_length_1063_cov_2.886905, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000575 DAOQAN010000000
VERSION     DAOQAN010000575.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_228248_length_1063_cov_2.886905"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1057)
                     /locus_tag="PKO10_08020"
     CDS             complement(<1..1057)
                     /locus_tag="PKO10_08020"
                     /inference="COORDINATES: protein motif:HMM:NF019974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate- and nitrite sensing domain-containing
                     protein"
                     /protein_id="HNW67131.1"
                     /translation="MTAGFIGSKGVKFKDELPRQREEVNKQITFLNEFLSDFDKSKYS
                     DDFVKNLNSGLEKLKDLQGVRNSVNSLNIAAPIAIAYYTDTNTLLLNTLGTIVKFSNS
                     PNVTKELGSYMNFLLSKERTGIERAVGTNTFAQNKFTEGLKARFYTLIAEQTAYLDIF
                     SKIASKDIVDFYLKTVVGKDIEEVEKMRKIALFSNIEENFGVDPNYWFETITNKINLL
                     KQVEDHISTHLLNEINLEKDEATFHLILFSLLSIFGIGITMILARTIAFTILIDVASV
                     RAGIEEFFAFINFEKDDIKLIGVDSTDELGMMSKIINKNIANTKANIQRDRELIADTI
                     RVANSINKGHLDQKIMVN"
BASE COUNT          349 a          183 c          101 g          430 t
ORIGIN      
        1 aattaaccat tattttttgg tctaaatgcc ctttattaat actatttgca actcttattg
       61 tatctgctat taattctcta tctctttgaa tatttgcttt agtatttgca atatttttat
      121 ttataatttt agacatcatt cctaactcat ctgttgaatc aacaccaatt aatttgatat
      181 catctttttc aaaattaata aaagcaaaaa actcttcaat tccagctctt acactagcaa
      241 catctattaa aattgtaaat gcaattgttc ttgccaaaat catagtaata ccaataccaa
      301 atatacttaa tagtgaaaaa agtattagat gaaaagttgc ttcatctttt tctaaattaa
      361 tctcatttag aagatgagtc gaaatatgat cttcaacttg cttcaaaaga tttatcttgt
      421 ttgtaattgt ttcaaaccag taatttggat ctactccaaa attctcttca atattgctaa
      481 aaagtgctat ttttcgcatc ttttcaacct cttctatatc ttttccaaca actgttttca
      541 aataaaaatc aactatatct tttgaagcaa tttttgaaaa aatatccaaa tatgcagttt
      601 gttcagctat aagagtataa aatctagctt ttaatccttc tgtaaactta ttttgtgcaa
      661 aagtatttgt accaactgct ctttctattc ctgttcgctc ttttgaaagt aaaaagttca
      721 tataacttcc caactctttt gtaacatttg gactatttga aaactttaca atagttccta
      781 aagtattaag caaaagagtg tttgtgtccg tatagtaagc tattgctata ggtgctgcaa
      841 tatttaaact atttacacta tttcttacac cttgtaaatc ttttaatttt tctaaaccac
      901 tatttagatt ttttacaaaa tcatcactat attttgattt atcaaaatct gataaaaatt
      961 catttaaaaa agttatttgt ttgtttacct cttctctttg tcttggtaac tcatctttaa
     1021 acttaacacc tttacttcca ataaatccag ctgtcatacc tct
//
LOCUS       DAOQAN010000576         1063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_228334_length_1063_cov_2.331349, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000576 DAOQAN010000000
VERSION     DAOQAN010000576.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_228334_length_1063_cov_2.331349"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="PKO10_08025"
     CDS             <1..197
                     /locus_tag="PKO10_08025"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014469051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HNW67132.1"
                     /translation="KNVVLTGAMLPISINKIEATLNFSQAIGFLNSNCENGIYISMHG
                     SVKNYKKLIKNRELGKFLEK"
     gene            complement(221..640)
                     /locus_tag="PKO10_08030"
     CDS             complement(221..640)
                     /locus_tag="PKO10_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014475466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family metal-binding protein"
                     /protein_id="HNW67133.1"
                     /translation="MKLSVNSICPCGSLKKYKKCCKIFHDDIKKPSNALELMKSRFSA
                     FAFCKSDYIIKTTHQKNCDFSLNTSSWIADIEKFCKNTSFENLEILDFIDSEIESFVT
                     FKATLFQNKKDISFIEKSRFLKENNIWLYVDGEFLNS"
     gene            787..>1063
                     /locus_tag="PKO10_08035"
     CDS             787..>1063
                     /locus_tag="PKO10_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014474761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HNW67134.1"
                     /translation="MALYTCGHIIPDSDSVCSAISLAYLLNKIGRAATPARQGELNPE
                     TKFILDKFGFEAPVLKTSFAGDELFITDYSDIAQAPQDLDKTTVVGIV"
BASE COUNT          409 a          141 c          129 g          384 t
ORIGIN      
        1 ataaaaatgt agttttaaca ggagctatgc ttccaatttc tataaacaaa atagaggcaa
       61 ctttgaattt ttcacaagca attggatttc ttaactcaaa ttgtgaaaat ggtatctata
      121 tctcaatgca tggaagtgta aaaaactata aaaaacttat aaaaaataga gagctaggaa
      181 aatttttaga gaaataaata tattaaaaat ttctctaatt ttagctattt aaaaactctc
      241 catctacata aagccaaata ttattctctt ttaaaaatct acttttttct ataaaagaga
      301 tatctttttt attttgaaac aaagtagctt taaatgttac aaagctttca atttcactat
      361 ctataaaatc taaaatctct agattttcaa aactagtatt tttacaaaat ttctcaatat
      421 cagctatcca agatgaggta tttaaagaaa aatcgcagtt tttttgatga gttgttttta
      481 taatataatc acttttacaa aaagcaaaag cactaaatct tgatttcata agctctaaag
      541 cgtttgaagg tttttttata tcatcatgaa aaattttaca acatttttta tattttttta
      601 aacttccgca aggacaaata gagtttacac taagtttcaa aataatcctt tttaatcttt
      661 atataagagt aatatttaaa caaagctgtt taaatttatt aagaatctaa ttttaaaaga
      721 aatttacctc tttttggata aaatcaatgc ttaaaaatta aaaaatataa aaaaaggata
      781 caacctatgg cattatatac atgtggacat attattccag attcagactc tgtttgttca
      841 gctatctctt tagcatatct attaaataaa attggaagag ctgcaactcc tgcacgacaa
      901 ggagagttaa atccagagac aaaatttatt ttagataagt ttggttttga agcgccagtt
      961 ttaaaaactt catttgcagg tgatgagctt tttattacag attactctga tattgctcaa
     1021 gctccacaag atttagataa aacaacagtt gttggaattg ttg
//
LOCUS       DAOQAN010000577         1060 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_229375_length_1060_cov_2.313433, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000577 DAOQAN010000000
VERSION     DAOQAN010000577.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_229375_length_1060_cov_2.313433"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            158..>1060
                     /locus_tag="PKO10_08040"
     CDS             158..>1060
                     /locus_tag="PKO10_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW67135.1"
                     /translation="MKLIYKIEYNSTSFYFKRLIDELIKNSNIDANTKQYFGFILIFI
                     DDELENIEKFFLNLETNLPYSMFLKKSYLIDEFNEEIKELEDKNIKENFEILTNRKVK
                     DILENSNFDFLSQIVNLNRSFVVKFEDLELFLPNKNLKENFEKDNYEVRLLVTNSQIL
                     TDLFIIEEPEINLLCSIERPLVKLKLKNIDENISNSGYIFTRLVNSSKEVELSKALKE
                     QNIDYILYVTKKDELKACSFENLNLIISDDKTLYPKYDYKKDLIFNSSSEYLNSFSNI
                     YNAVLHEHNLLDKTSIGVYFSLNSK"
BASE COUNT          433 a           96 c          143 g          388 t
ORIGIN      
        1 ccatttttta tagaaacttc tataaaagag atagagagct taggttttaa atttacaaaa
       61 aaatctgata ttgctgttcc tgatattgta aaaagtttaa ttggaagcta caatggctct
      121 catttaaata gaatcccaaa tgaagaggat tttactcatg aagttaatct ataaaataga
      181 gtacaactca acttcttttt attttaaaag attaatagat gagttgataa aaaactcaaa
      241 tatagatgca aatacaaaac aatattttgg atttatttta atatttattg atgatgagct
      301 tgaaaatatt gaaaagtttt ttttaaattt agaaacaaat cttccttact ctatgttttt
      361 gaaaaaatca tatttaatag atgaatttaa tgaagagatt aaagagttag aagataaaaa
      421 tataaaagag aattttgaga ttttgacaaa tagaaaagtt aaagatattt tagaaaattc
      481 taattttgat tttttaagtc aaattgttaa tttaaacaga tcttttgttg ttaaatttga
      541 agatttagaa ctctttttac caaataaaaa tttaaaagaa aactttgaaa aagataatta
      601 tgaagttagg ttgttagtta caaatagtca gattttaaca gatcttttta taattgaaga
      661 gcctgaaata aatcttttat gctcaataga aagacccttg gttaaattga aattaaaaaa
      721 tatagatgaa aatatttcaa atagtggata tatttttaca agacttgtaa actcttcaaa
      781 agaggttgag ctatcaaaag cgctaaaaga acaaaatatt gattatattt tatatgtgac
      841 taaaaaagat gagttaaaag cttgtagttt tgaaaatcta aatcttatta ttagtgatga
      901 taaaactttg tatccaaaat atgattataa aaaagatttg atatttaatt caagtagtga
      961 gtatttaaac tctttttcga atatttataa tgcagtttta cacgagcata accttttaga
     1021 taaaacctct attggagtct atttttcact gaattctaag
//
LOCUS       DAOQAN010000578         1058 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_230220_length_1058_cov_1.511466, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000578 DAOQAN010000000
VERSION     DAOQAN010000578.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_230220_length_1058_cov_1.511466"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="PKO10_08045"
     CDS             complement(<1..233)
                     /locus_tag="PKO10_08045"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HNW67136.1"
                     /translation="MLKCDLLVDSKDLRATLLKLKKDDDFTILLDMTAVDYSKFPDST
                     PSRFAVVYVLRSSDFKKETTVKSFVSDETLSVDT"
     gene            complement(226..>665)
                     /gene="nuoB"
                     /locus_tag="PKO10_08050"
     CDS             complement(226..>665)
                     /gene="nuoB"
                     /locus_tag="PKO10_08050"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HNW67137.1"
                     /translation="SYSLWPMAFGTACCGIEFMAVAAARYDVSRFGAEVVRFSPRQAD
                     LLIVAGTISYKQAPILKKIYEQMCEPKWVISMGACACSGGFYDNYATVQGIDEIIPVD
                     EYVAGCPPRPEAVLDAIMRIQEKAKNESIIKDRVKEYKGFLDA"
     assembly_gap    666..675
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<676..749)
                     /locus_tag="PKO10_08055"
     CDS             complement(<676..749)
                     /locus_tag="PKO10_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HNW67138.1"
                     /translation="MGLGVESSLGDSILTTKLDAAINWG"
     gene            complement(740..1051)
                     /locus_tag="PKO10_08060"
     CDS             complement(740..1051)
                     /locus_tag="PKO10_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002848151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HNW67139.1"
                     /translation="MLAVVFMLTKFIGPQNKDSVLKNNVYESGVTNPVGTTNNRFSVK
                     FYLVAISFLLFDVEVIFMFPWAVNVVDLGVAGLFKMFIFMGLLFIGLIYIYKKKALSW
                     D"
BASE COUNT          382 a          212 c          122 g          332 t
ORIGIN      
        1 gtatcaacac ttaaagtttc atcacttaca aatgatttta ccgtagtctc ttttttaaaa
       61 tcactacttc ttaaaacata aacaactgca aatcttgatg gagtagagtc tggaaatttt
      121 gaataatcaa ctgctgtcat atctaaaaga attgtaaaat catcatcttt ttttaatttt
      181 aaaagagttg ctcttaaatc ttttgaatca actaataaat cacatttaag catctaaaaa
      241 tcctttatac tctttaactc tatctttaat aattgactca tttttagctt tttcttgaat
      301 tctcatgatt gcatcaagaa cagcttctgg tcttggtgga caaccagcaa catactcatc
      361 aactggaata atttcatcaa taccttgaac tgttgcataa ttgtcataaa aaccaccact
      421 acaagcacat gcacccatag atattaccca ttttggttca cacatttgct cataaatttt
      481 tttcaaaata ggagcttgtt tatatgaaat agttccagca actattaata aatctgcttg
      541 tcttggagag aatcttacaa cttcagctcc aaatcttgaa acatcatatc tagcagcagc
      601 aactgccata aactcaatac cacaacaagc tgttccaaat gccataggcc aaagagaata
      661 tgaccnnnnn nnnnncccca gttaatagct gcatctaact ttgtagtaag tatgctatct
      721 cctaaactcg attcaactcc taatcccatg ataaagcctt tttcttatag atataaatta
      781 atccaataaa taacagtccc ataaagataa acatcttaaa tagtccagca actccaagat
      841 caacaacatt tacagcccaa ggaaacataa aaatcacctc tacatcaaaa agtaaaaatg
      901 agatagcaac caaatagaat tttacagaga atctattgtt tgttgttcca acaggatttg
      961 taactccact ttcatagaca ttgtttttaa gaacagagtc tttattttga ggaccaataa
     1021 atttggtaag cataaaaaca actgcaagaa ttacccca
//
LOCUS       DAOQAN010000579         1057 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_230505_length_1057_cov_1.786427, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000579 DAOQAN010000000
VERSION     DAOQAN010000579.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1057
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_230505_length_1057_cov_1.786427"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1039
                     /locus_tag="PKO10_08065"
     CDS             <1..1039
                     /locus_tag="PKO10_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013136869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HNW67140.1"
                     /translation="RFSALNGICKINFKAFLKFCITIGLLLDIKENGKVYPLSNEAKS
                     VTNLFSLALEELNVNIFCEHFVQKVEKKDDKFIIYANDKEFKSYDKVLISSGLAAAPQ
                     LNATEIGLEIASSFGHTFNPTYPSLVGLQTKDTYKGKLQGVKKECSVSLYINGNSEQE
                     IFGDVLFTSYGVSGFAILDISQRAVLALSKFYDVELRVNFFPKTSINDLANQIQTLFK
                     NLPKQKAVDILTGLISNKIAPILLEICKIDINTKADDINTKQIKSLAHQLNSWRLKVV
                     DTQGFSHAEASGGGVRTCEVDNKNYESKLIKNLFFAGEVLDIVGNRGGFNLHFAWASG
                     YLVGKNLITDK"
BASE COUNT          396 a          119 c          185 g          357 t
ORIGIN      
        1 aagattttca gctcttaatg gtatatgtaa aattaatttt aaagcatttc ttaagttttg
       61 tataactatt ggacttttac tagatattaa agaaaatggt aaggtttatc cactatcaaa
      121 tgaagcaaaa tctgtaacaa atcttttttc tttggcttta gaagagttaa atgtaaatat
      181 attttgtgaa cactttgtac aaaaagtaga aaaaaaagat gataaattta taatttatgc
      241 aaatgataaa gagtttaaat cctatgataa agttttaatt tcaagtggat tagctgctgc
      301 tcctcaacta aatgcaactg aaattggtct tgaaattgca tcaagttttg gacacacttt
      361 taatcctaca tatccaagcc ttgttggact tcaaacaaaa gatacataca aaggaaaact
      421 tcaaggagtt aaaaaagagt gtagtgtgag tttgtacata aatggaaatt ctgagcaaga
      481 gatttttgga gatgttctgt ttacatctta tggtgtgtct ggatttgcta ttttagatat
      541 ctctcaaaga gctgttttgg ctttgagtaa attttatgat gttgaactta gagttaattt
      601 ttttcctaaa acttctataa atgatttggc aaatcaaata caaactttgt ttaagaattt
      661 accaaagcaa aaagcagttg atattttaac tggacttata tcaaataaaa tagctccaat
      721 acttttagag atttgtaaaa ttgatataaa cacaaaagca gatgatataa atacaaaaca
      781 gataaaatca cttgctcatc agctaaactc atggagatta aaagtagtag atactcaagg
      841 attttctcat gcggaagcaa gtggtggagg agtgcgaact tgtgaagtag ataataaaaa
      901 ttatgagagt aaattaataa aaaacctatt ttttgctgga gaggttttgg acatagttgg
      961 aaatagaggt ggatttaatc ttcactttgc atgggctagt ggttatttgg tggggaaaaa
     1021 tttaatcaca gataaataaa ttaaaggaaa attataa
//
LOCUS       DAOQAN010000580         1057 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_230541_length_1057_cov_1.533932, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000580 DAOQAN010000000
VERSION     DAOQAN010000580.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1057
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_230541_length_1057_cov_1.533932"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..948)
                     /locus_tag="PKO10_08070"
     CDS             complement(<1..948)
                     /locus_tag="PKO10_08070"
                     /inference="COORDINATES: protein motif:HMM:NF012307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase/maturase family protein"
                     /protein_id="HNW67141.1"
                     /translation="MCGLSVIVGGNWNNAAISGVFAMNLNNARSNSNDNIGGRDFNSL
                     PDTTMVDTGVIGVCCPAISEIKKDTSLSSRLNLESQGVSQKSKRVGFLCEKAFTREAL
                     YEAYLVARKGKRKKRATLSFEKRLGAELDSLYNELQNGTYKPKPYVTFYVYEPKKREI
                     KAPAFRDLVVQHAIYKVIYPIFDRKFIHTSYACRKGGGTHKASAYTQKEIRKYSGNDY
                     FAKLDIKKFFYSIMRFILEKMFYKVIKDKRFIAIMMMFAEIPTLFGIPIGNLLSQLYA
                     LIFLNPLDHFVKRVLKVKSYVRYVDDFVAIGLNLEKAKEF"
BASE COUNT          349 a          209 c          120 g          379 t
ORIGIN      
        1 aaactctttt gctttttcta aatttaagcc aatcgctaca aaatcatcta catatctaac
       61 atagctttta acttttaaaa ctctttttac aaagtgatcc aaaggattta aaaatataag
      121 tgcatataat tgactaagta gatttccaat aggtattcca aacaaagttg gtatctctgc
      181 aaacatcatc atgattgcaa taaatctttt atctttgata accttataaa acatcttttc
      241 taaaataaat ctcataatag agtaaaagaa ttttttaata tcaagttttg cgaaataatc
      301 attaccgcta tattttctta tctctttttg agtgtaagca cttgctttgt gagtacctcc
      361 accttttcta caagcataac ttgtatgtat aaattttcta tcaaaaatag gatagataac
      421 tttatatatt gcatgttgaa caactaaatc tctaaatgca ggagctttaa tttctctttt
      481 ttttggctca taaacataaa aggttacata gggctttggt ttataagttc cattttgtag
      541 ctcattatat aaactatcca gttcagcacc taatcttttc tcaaagctta gtgtagctct
      601 cttttttctt ttgccttttc tagctactaa ataggcttca tataaagctt ctcttgtaaa
      661 agctttttca cataaaaatc caactctttt agatttttgc gatactccct gactttcgag
      721 atttaaccta ctagaaagag atgtatcttt tttgatttcg cttatagcag gacaacatac
      781 ccctataact ccagtatcta ccattgtagt atcaggtaaa gagttaaagt cacgaccacc
      841 aatattgtca ttcgaattcg accgagcatt attcaaattc atagcgaaca cacccgaaat
      901 agcagcatta ttccaattac cgccaacaat aaccgacaag ccacacaccg catattgtta
      961 cattgcccta aaattaaatc tctcacgaga aaagtccttg cccttttaat ttatttatcc
     1021 aagcacctat taatcttcca atttcatcaa taaactt
//
LOCUS       DAOQAN010000581         1056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_230583_length_1056_cov_15.820180, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000581 DAOQAN010000000
VERSION     DAOQAN010000581.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_230583_length_1056_cov_15.820180"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="PKO10_08075"
     CDS             complement(<1..331)
                     /locus_tag="PKO10_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW67142.1"
                     /translation="MNNQVFEEMKSIAKDYDIEFSDENDIEKCKQEFTDKVSWYYISK
                     YQKLSEGLIREFADKVDWYYISEYQKLSEGFIQEFTDKVSWYYISKYQKLSEGFIREF
                     ADKVNWHN"
     gene            complement(347..>1056)
                     /locus_tag="PKO10_08080"
     CDS             complement(347..>1056)
                     /locus_tag="PKO10_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW67143.1"
                     /translation="SKYQKLSEEFIKEFSDKVDWFHISINQKLSEEFIREFSDKIYWE
                     YISEYQKLSEEFIREFANKVDWYYISKYQKLSEEFIKEFSDKVNWDSISAYQKLSESF
                     IKEYKLTIPKTNWQYKIKEEKLSYLKENCNGIYEIVDDNYIVAYKSTRVDGYSTYNFQ
                     YQYKVNEVYESHCDCNTDDENSFGLSAWTKEKALEYYNEGKLFKVHINIEDIGAIVHD
                     NKKIRCSKLTVISEESF"
BASE COUNT          345 a          161 c           94 g          456 t
ORIGIN      
        1 tattatgcca atttacttta tcagcaaatt ctcgaataaa tccttctgat agtttctgat
       61 attttgaaat ataataccaa cttactttat cagtaaattc ttgaataaat ccttctgata
      121 gtttttgata ttctgaaata taataccaat ctactttatc agcaaattct cgaatcaatc
      181 cttctgatag tttctgatat tttgaaatat aataccaact tactttatca gtaaattctt
      241 gtttacattt ttcaatatca ttttcatctg aaaactcaat atcataatct tttgcaattg
      301 atttcatttc ttcgaaaact tgattgttca ttatatttct tcttattcaa aaagattctt
      361 cagaaataac tgttagttta ctgcatcgaa ttttcttgtt atcatgaaca attgctccaa
      421 tatcttctat attgatatga actttgaata gttttccttc attataatat tccaaggctt
      481 tttcttttgt ccatgctgat aatccaaatg aattctcatc atctgtattg caatcacaat
      541 gagattcata tacctcattt accttatatt gatattgaaa attatatgta gaatatccat
      601 caactcttgt tgatttatat gctactatat aattatcatc tacaatttca taaataccat
      661 tacaattttc tttcaaataa gatagctttt cttcctttat tttatattgc caattagttt
      721 ttggtattgt taatttgtat tctttaataa aagattctga tagtttttgg taagctgaaa
      781 tactatccca attcacttta tcagaaaatt ctttaataaa ttcttctgat agtttctgat
      841 attttgaaat ataataccaa tctactttat tagcaaattc tcgaataaat tcttctgata
      901 gtttttgata ttctgaaata tattcccaat atattttatc agaaaattct cgaataaatt
      961 cttctgataa cttttgatta attgaaatat gaaaccaatc tactttatca gaaaattctt
     1021 taataaattc ttctgatagt ttttgatatt ttgaaa
//
LOCUS       DAOQAN010000582         1056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_230747_length_1056_cov_2.337662, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000582 DAOQAN010000000
VERSION     DAOQAN010000582.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_230747_length_1056_cov_2.337662"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="PKO10_08085"
     CDS             <1..299
                     /locus_tag="PKO10_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014474802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HNW67144.1"
                     /translation="TAEIFASELGYNKTIMLNEVLYMAFVNELIETITYTYDTVDTML
                     LVGHNPSLTALAVTLLGFKEKFEEGAVMQIEFNCNSWIDIDKNNAKLISYTIPK"
     gene            complement(318..728)
                     /locus_tag="PKO10_08090"
     CDS             complement(318..728)
                     /locus_tag="PKO10_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW67145.1"
                     /translation="MKRVLIFVLLFLSANVYADKWDDFLKDSGFPKKVIDDGSLVSGK
                     ILDATVSYQIKNNDGIKGNFENSDSAKVIIKNKKGQTLSTEKFLNVSELRVWGRDNFI
                     EIFSFVIGDRKLDSKTLNELTNLATVALMNKNIK"
     gene            complement(730..>1056)
                     /locus_tag="PKO10_08095"
     CDS             complement(730..>1056)
                     /locus_tag="PKO10_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HNW67146.1"
                     /translation="RTFEKELLSEFIELSTKESINLLIKIIEKILDENSGTDELTLVS
                     KRQINSIENTLQNIKMAKNPLYSGELEFFAHHITEALHEISNITRPYENDEMLDVMFG
                     EFCLGK"
BASE COUNT          365 a          172 c          108 g          411 t
ORIGIN      
        1 atacagcgga aatttttgca agtgagttag gatataacaa aactattatg ctaaatgaag
       61 ttttatatat ggcatttgta aatgagctaa tagaaactat cacatatact tatgatacag
      121 ttgatactat gttgctagta ggtcataatc catctttaac tgctttagca gttacacttt
      181 taggatttaa agagaaattt gaagagggtg cagttatgca aatagagttt aattgtaata
      241 gctggattga tattgacaaa aataatgcaa aactaataag ctatactatt cctaaatagt
      301 ttagcttaac tattaattta ctttatattt ttattcatca aagcaacagt tgcaagattt
      361 gtaagctcat ttaatgtttt cgaatctaat tttctatcac ctataacaaa actaaaaatc
      421 tcaataaaat tatctcttcc ccaaactcta agctcagaaa cattcaaaaa cttttcagta
      481 cttaaagttt gacctttctt atttttaatt ataacttttg cactatcact attttcaaag
      541 tttcctttta ttccatcatt atttttaatt tgatagctta cagttgcatc taaaatctta
      601 ccacttacca aacttccatc atctataact ttttttggaa aaccactatc ttttaaaaaa
      661 tcatcccatt tatccgcata aacatttgca gataaaaaga gtaaaacaaa aatcaaaact
      721 cttttcatac tattttccta aacaaaattc accaaacata acatctaaca tctcatcatt
      781 ttcatatggc cttgtaatat ttgatatttc atgtaaagct tctgttatat ggtgtgcaaa
      841 aaactctagt tctccactat acaatggatt ttttgccatt tttatatttt gtagtgtatt
      901 ttctatacta tttatttgtc tttttgaaac aagtgtaagc tcatctgttc cactattttc
      961 atctaatatt ttttctataa ttttaataag taaattaata ctctcttttg ttgaaagctc
     1021 tataaattca ctcaataact ctttttcaaa agttct
//
LOCUS       DAOQAN010000583         1055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_231020_length_1055_cov_3.197000, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000583 DAOQAN010000000
VERSION     DAOQAN010000583.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_231020_length_1055_cov_3.197000"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="PKO10_08100"
     CDS             <1..309
                     /locus_tag="PKO10_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013135054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase-related domain-containing protein"
                     /protein_id="HNW67147.1"
                     /translation="EPTKAFYEPNSFDGPVEDKSYLEPDLAVGDIAKRYDHRVGNDDF
                     SQPRALFLLMSNSQKEQLFSNIKDAMAGVPRDIVDRQIALFEKVHPDYAAGVKKALGI
                     "
     gene            318..800
                     /locus_tag="PKO10_08105"
     CDS             318..800
                     /locus_tag="PKO10_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014474738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNW67148.1"
                     /translation="MEVSQEELKRYEELQVLALDFARVGKTQDLKAMLEAGMSVNLTD
                     HKGNTLLMLASYNGNFETTKMLLEYKAEVDRKNDRGQTPLAGVCFKGYFDIVKILVEA
                     GANIHENSGMGTTAITFASMFGHTKIVEYLNKQNKNRSFKSKIYLLLSKFISLFKKNK
                     "
BASE COUNT          409 a          115 c          188 g          343 t
ORIGIN      
        1 gagcctacaa aggcatttta tgagccaaat agctttgatg gaccagttga agataaaagt
       61 tatttagagc cagatttagc tgttggagat attgcaaaaa gatatgacca tagagttgga
      121 aatgatgatt tttctcaacc aagagcacta tttttactta tgagtaatag tcaaaaagag
      181 caactatttt caaatataaa agatgcaatg gctggtgtac caagagatat tgttgataga
      241 caaattgcac tttttgaaaa agtacatcca gattatgcag ctggtgttaa aaaagcttta
      301 ggaatatagg tttttatatg gaagtttcac aagaagagtt aaagcgatat gaagagctac
      361 aagttttagc acttgatttt gcaagagttg gtaaaacgca agatttaaaa gctatgcttg
      421 aggctggaat gagtgttaat ctaacagatc acaaaggcaa tactcttttg atgttagctt
      481 catataatgg aaattttgaa actactaaga tgttattaga atataaagca gaagtagata
      541 gaaaaaatga tagaggacaa acaccacttg ctggtgtttg tttcaaaggc tattttgata
      601 ttgtaaaaat tttagttgaa gctggtgcaa atattcatga aaatagtgga atgggcacaa
      661 ctgctataac atttgcttca atgtttggac atacaaaaat tgttgaatat ctaaacaaac
      721 agaataaaaa tagaagtttt aaatcaaaaa tatatctact tttatcaaaa tttatatcgc
      781 tatttaaaaa gaataaataa tatccatttt ataaatttta ttactaatta gtaattagct
      841 taagaataaa ctagctacaa ttctaaaaat tttttaaagg attaattatg aagagttgtg
      901 aaaataaact atcttgtatg ctaaatgaag atataggaaa actaatttta agagtaagta
      961 tagcaggact tatgctattt catggatttt ttaaattatt taatggaata gatggaatta
     1021 aatacttggt tactcaagct ggattaccag agttt
//
LOCUS       DAOQAN010000584         1053 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_231902_length_1053_cov_2.033066, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000584 DAOQAN010000000
VERSION     DAOQAN010000584.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_231902_length_1053_cov_2.033066"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="PKO10_08110"
     CDS             <1..190
                     /locus_tag="PKO10_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW67149.1"
                     /translation="TTDKKYQYAITTIFNEVNYDDVIKYCEIFNIDKTNLKTVYEYYK
                     DQYNAKEISEYEWLFKQF"
     gene            165..863
                     /locus_tag="PKO10_08115"
     CDS             165..863
                     /locus_tag="PKO10_08115"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HNW67150.1"
                     /translation="MSGYLNNFEQQISTLNSVYYELLAPIEKQSTLKNWWVFGGGTAL
                     AMFHFNHRKSFDIDIFITESQLFDFLNPKWYIDETNLFDSNEYRFDGINHHVQLKTKD
                     DIKVDFLLNESIINNPIENKIIDLDYKLFYESIEDIVAKKIKYRKQDNLTRDIFDIAV
                     AIFFDKKIIENLVFSRFISLDDLDILNSSLDRLDEKKYILELEKIEPQTKKFENIALN
                     AKNIIQKSIKSLEI"
     gene            complement(868..>1053)
                     /locus_tag="PKO10_08120"
     CDS             complement(868..>1053)
                     /locus_tag="PKO10_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011372985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW67151.1"
                     /translation="TQRDMAGYIGVATATIRNWKKDKPKLYEIVMKGFAFEEAVKKAQ
                     ENADELKALEEKFKIKK"
BASE COUNT          408 a          120 c          137 g          388 t
ORIGIN      
        1 aacaacagat aaaaaatatc aatatgcaat aactacaatt tttaatgaag taaattatga
       61 tgatgtaata aaatattgcg aaatttttaa tattgataaa acaaatctaa aaacagtata
      121 tgaatattat aaagaccagt acaatgcaaa ggagatttca gaatatgagt ggctatttaa
      181 acaattttga acaacaaata agtactttaa atagtgttta ttatgagttg ttagctccta
      241 ttgaaaaaca gtcaactttg aaaaattggt gggtttttgg tggtggcact gctttagcaa
      301 tgtttcattt taatcacaga aaatcatttg atatagatat atttatcaca gaatcacaac
      361 tttttgattt tttaaatcca aaatggtaca tagatgagac aaatttattt gatagcaatg
      421 aatacagatt tgatgggata aatcaccatg tacaacttaa aactaaagat gatataaaag
      481 tagatttttt acttaatgaa tcaattataa acaatcctat agagaataaa attatagatt
      541 tagattataa acttttttat gaatcaattg aagatatagt agcaaaaaaa ataaagtata
      601 gaaaacagga taatcttact cgtgatattt ttgatattgc agtggctata ttttttgata
      661 aaaaaattat tgaaaattta gttttttcaa gatttatttc cttggatgac ttagatattt
      721 taaactcatc tttagataga cttgatgaga aaaaatatat tctagagctt gaaaaaattg
      781 aaccacaaac taaaaagttt gaaaatatag ctttaaatgc taaaaatata attcaaaaaa
      841 gtattaaatc tttagaaata taaaatttta tttttttatt ttaaatttct cttccaaagc
      901 ttttagctca tctgcatttt cttgtgcttt ttttacagcc tcttcaaagg cgaagccttt
      961 cattactatt tcataaagtt ttggtttgtc ttttttccag tttcttatag ttgcagttgc
     1021 aactcctata tatccagcca tatctctttg agt
//
LOCUS       DAOQAN010000585         1051 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_232444_length_1051_cov_3.715863, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000585 DAOQAN010000000
VERSION     DAOQAN010000585.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_232444_length_1051_cov_3.715863"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="PKO10_08125"
     CDS             <1..242
                     /locus_tag="PKO10_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW67152.1"
                     /translation="EFYKLICEDLNLDFEEFSKLFEDKSLDKKLQNEFIFGRNLSSSF
                     PSLILVNKKQKVNISIGYSSLEEVISRINKNLKSF"
     gene            complement(266..>1051)
                     /gene="gltS"
                     /locus_tag="PKO10_08130"
     CDS             complement(266..>1051)
                     /gene="gltS"
                     /locus_tag="PKO10_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HNW67153.1"
                     /translation="ILETKYGIEGAMGLGMAAATFGLVMGGIIGGPLAKFLINRYKLS
                     NEKELQQNNSKDEKVIDEFVPFEYPKAVRLITTNSAISTLALFAACLAFADFITNLTK
                     GSAFELPTFVWALAGGVILRNSLENILKIEIFDRAIDVFGNASLSLYLAMALLSLKLW
                     QLSNLAGALTVILISQVLIMILYAYFVTFRVMGKNYDASVLAAGHCGFGLGATPTAVA
                     NMQAITNMYGPSHRAFLIVPLCGAFFVDLINTTVIQTIFKIFE"
BASE COUNT          397 a          160 c          133 g          361 t
ORIGIN      
        1 tagagtttta taaattaatt tgtgaagatt taaatcttga ttttgaagag ttttctaagc
       61 tatttgaaga taaatctttg gataaaaaac ttcaaaatga gtttatattt ggaagaaatt
      121 taagtagttc tttccctagt ttaattcttg taaataaaaa acaaaaagta aatatatcta
      181 ttggatatag tagtttagaa gaggtaattt caagaataaa caaaaaccta aagagcttct
      241 aaaagttctt tagattttta aataattact caaaaatttt aaatatagtt tgaataactg
      301 tagtatttat taaatctaca aaaaatgctc cacataatgg aactattaaa aatgctctat
      361 gcgaaggtcc atacatattt gtaattgctt gcatatttgc aactgctgta ggagttgctc
      421 ctaaaccaaa accacaatgt ccagctgcta aaacacttgc atcataattt tttcccataa
      481 ctctaaatgt aacaaaataa gcatataaaa tcataataag aacttgagaa attaaaatta
      541 cagttaaagc accagctagg tttgatagtt gccaaagttt taaagatagt aaagccattg
      601 caagatataa agataatgat gcatttccaa atacatcaat agctctatca aaaatctcaa
      661 tttttaggat attttctaaa ctatttctta aaattacacc acctgctaat gcccaaacga
      721 aagtaggtag ttcaaatgct gaaccttttg ttaaatttgt tataaaatca gcaaaagcca
      781 aacaagctgc aaaaagagct aaagttgaaa ttgcactatt tgtagttatt aatcttactg
      841 cttttggata ttcaaatgga acaaactcat caattacttt ctcatcttta gagttatttt
      901 gttgtaactc tttttcattt gaaagtttat atctatttat taaaaatttt gctagaggtc
      961 ctccaataat tccacccatt acaagaccaa aagttgcagc tgccattcca agccccatag
     1021 ctccttcaat tccatatttt gtctctaata t
//
LOCUS       DAOQAN010000586         1047 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_233926_length_1047_cov_2.935484, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000586 DAOQAN010000000
VERSION     DAOQAN010000586.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_233926_length_1047_cov_2.935484"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1005)
                     /locus_tag="PKO10_08135"
     CDS             complement(<1..1005)
                     /locus_tag="PKO10_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013134711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNW67154.1"
                     /translation="MNVKKITMLSFVVATALNANEATTLNEITIVEKSNSKLIKDINS
                     EELKSADLAEALTKNSANISVVRRNGIANDIILRGQKKDNINILIDNAKIYGACPNRM
                     DPPTSHVVTNNIKSVKIIEGPYDVENFGTLSGLVKVETKDPKEGFHGELNLNAGSFNY
                     KKASATIEGGNDKVKALISASTEESKAYKDGNGDNFLEQQRKRGVPIATQYRDNNIDA
                     FEKKSVLTKLQFNITDDQDLRLSYTANRSDGILYPAGPMDADYDDSDIYTLGYTIRDL
                     GAFSKQLDLDYYYSKVDHPMSGKLRNGNGTSYMTNHLKTSIWGTTVKNSLEVADSLVT
                     V"
BASE COUNT          317 a          182 c          117 g          431 t
ORIGIN      
        1 aactgttact aaactatctg caacttctaa agagttttta actgttgttc cccaaattga
       61 agtttttaga tggtttgtca tatatgaagt tccatttcca tttcttaact ttccactcat
      121 aggatgatct actttagagt aataataatc taaatctagc tgttttgaaa aagcccccaa
      181 atctcttatt gtatatccta aagtataaat atcactatca tcataatcag catccatagg
      241 accagctgga tataaaatac catcacttct atttgcagta taagatagtc ttaaatcctg
      301 atcatctgta atattaaatt gaagttttgt taaaacactt tttttctcaa atgcatcaat
      361 attattatct ctatattgag ttgcaatagg aactcctctt tttctttgtt gttctaaaaa
      421 gttatcacca tttccgtctt tatatgcttt actctcttct gttgaagcag agattaaggc
      481 tttaacttta tcatttccac cttcaattgt tgcacttgct tttttatagt taaaacttcc
      541 tgcatttaaa ttcaactcac catgaaatcc ctcttttgga tcttttgttt caacttttac
      601 aagaccactt aaagttccaa aattttcaac atcataagga ccttcaataa tctttacact
      661 tttaatatta tttgtaacca catgagaagt tggtggatcc attctatttg gacaagctcc
      721 atatattttt gcattatcta ttaagatatt aatattatct tttttttgtc ctcttaaaat
      781 aatatcattt gcaattccat ttcttcttac aactgagata ttagcagagt ttttagttaa
      841 agcttcagct aaatcggcac tttttagctc ttcactattt atatctttta taagttttga
      901 gttagatttt tctactatag ttatctcgtt taaagttgtt gcttcatttg catttaaagc
      961 tgtagcaact acaaatgata gcatagtaat tttttttaca ttcataattt ttcccttttt
     1021 aaaaatttta gaagtattac aaagatg
//
LOCUS       DAOQAN010000587         1047 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_233942_length_1047_cov_2.745968, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000587 DAOQAN010000000
VERSION     DAOQAN010000587.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_233942_length_1047_cov_2.745968"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            136..885
                     /locus_tag="PKO10_08140"
     CDS             136..885
                     /locus_tag="PKO10_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNW67155.1"
                     /translation="MSEFISILTSTEIILGVITFLTSVIAAVVGIGGGMMLIAILPSF
                     LPLNALIPVHGLTQVSSNLSRAFFGYKDIQYEVIPKFLIGSVLGIGLFAGILSFISLE
                     YVPLFIGVYILLSLWSEKFNEKIKRYENYYLAGFFQTGLSMVVGATGPLTMTLLFKDY
                     QDKDKVVATGAALMSITHFLKVVVFIYFGFVFFDFIWIIIAMIFGAVAGSFVGTKARN
                     LIDGKKFTIILKTLLTILAIKLIVDIIVKII"
BASE COUNT          364 a          101 c          164 g          418 t
ORIGIN      
        1 aaatttgata aaaatatagt aataataaga agtaatttca tattttttcc tttttttaat
       61 ttaaagtata actatatttc aataataaag ggtttatttg atattttaca aaatcttttt
      121 aattgggaaa aatatatgtc agaatttatt tctattttaa cttcaacaga gattatttta
      181 ggagttatta cttttttaac ttcagttatt gcagctgttg ttggaatagg aggtggaatg
      241 atgttaatag caattttacc atcatttttg cctctaaatg cacttattcc agttcatgga
      301 ctcactcaag taagtagcaa cttaagccgt gctttttttg gatacaaaga tattcaatat
      361 gaagtaatac caaagttttt aataggttca gttttaggaa taggtttatt tgctggaatt
      421 ttgagtttta tctctcttga atatgttcct ttatttattg gagtctatat tttactttct
      481 ctttggagtg aaaaatttaa tgaaaagatt aaaagatatg aaaattacta tttagctgga
      541 ttttttcaaa ctggactttc tatggttgtt ggtgctactg gaccacttac tatgacacta
      601 ttatttaaag attatcaaga taaagataaa gttgtagcaa ctggagctgc tttgatgagt
      661 ataactcatt ttctaaaagt tgttgttttt atctattttg gttttgtatt ctttgacttt
      721 atttggatta ttattgctat gatttttgga gctgttgcag gaagttttgt tggaacaaaa
      781 gcgagaaatt taatagatgg taaaaaattt acaattattc taaaaacact acttacaatt
      841 ttagctataa aacttattgt agatattata gtaaaaataa tttagaattt aaaaggttat
      901 aaaatggagt taataagatt aaacagaagt cataaaaatg actttaagaa aataaacgat
      961 attacagaaa aagagttaga aaatccagag ttttttatag gtttttttac tgaagatgag
     1021 attgataata ttttagagaa ctcgata
//
LOCUS       DAOQAN010000588         1047 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_234076_length_1047_cov_1.972782, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000588 DAOQAN010000000
VERSION     DAOQAN010000588.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_234076_length_1047_cov_1.972782"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="PKO10_08145"
     CDS             complement(<1..434)
                     /locus_tag="PKO10_08145"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNW67156.1"
                     /translation="MISASWKGALAISFSAVLWGLDGVVLTPKLHTLDVFFVVLMLHA
                     IPFAVMSIFLPKKFKAILKFSPVELTALFLASLLGGAAGTIFIVKALFIIDFQPFTIV
                     ILLQKLQPVFAITLAAMLLKEKPGSKFLLWAGLAIAAGYFLT"
BASE COUNT          338 a          177 c          211 g          321 t
ORIGIN      
        1 aaggttaaaa agtatccagc agctattgct aatccagccc atagaagaaa tttacttcca
       61 ggcttctctt ttaaaagcat agcagcaagt gtaattgcaa aaacaggctg taatttttgt
      121 agtaggatca caatggtaaa tggctggaaa tctatgataa aaagtgcttt tacaatgaag
      181 atagtaccag ctgccccacc aagcaaagat gcaagaaaca aagcagttaa ctctaccggc
      241 gaaaatttca aaatggcttt gaatttttta ggtaggaaga tactcatcac tgcaaatggg
      301 attgcatgaa gcattaatac aacaaaaaag acatcaagag tatggagttt tggagttagt
      361 actactccat ctagccccca taaaacagct gaaaaactta tagctagagc ccctttccag
      421 cttgcagaaa tcattttgtt taccatcgat aattggattg tagcaaagat aagaaaagga
      481 tgctgtttat tgatgcatat ctatcacggt gttatctatt acaatcatcg ttaggttcta
      541 tcgctatgtt ttgtacctct ccattatctc tccctatctt ctccatattt tgagcgtcta
      601 ataaggtatg taatagattt agcatatgga ctaatatggg ctggctatcg cttaaccgtt
      661 gacgcatacc gggagagtga tggagtttta tccagatgga tttaatttac tgtttgccgc
      721 tttatccctc tcgttatttc tttttttcaa ggagtaaaaa tggacgaaag gaggcttata
      781 ggcagaggta acggagagag agcgaagagg tgatcgttaa tggttgtatt ataagatgtt
      841 gcattttgat gtttagtgac ctcatcaaat agaagaccat aactcaagag cgagttatgg
      901 tctatcatat aagttattgt attttaaata cttctctact ttctggggga aaagaagagc
      961 ttgataataa agtaaagggt tactattgac gctaccgtga aggtggcagt ccccattaaa
     1021 ctatcaggtc tataaatcat taatgta
//
LOCUS       DAOQAN010000589         1046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_234281_length_1046_cov_2.854692, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000589 DAOQAN010000000
VERSION     DAOQAN010000589.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_234281_length_1046_cov_2.854692"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /gene="rnc"
                     /locus_tag="PKO10_08150"
     CDS             complement(<1..420)
                     /gene="rnc"
                     /locus_tag="PKO10_08150"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014469064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HNW67157.1"
                     /translation="MSDYSKLEKCLDYQFKNKNLIIEALTHKSFKKPYNNERLEFLGD
                     AVLNLIVGEYLYTKFPKSNEGELSKIRASLVNETGFTKLANEINLGDYIFISNAEERN
                     KGRTKSSILSDAFEAIMGAIYLESGLETLKPIILNLLE"
     gene            complement(473..967)
                     /locus_tag="PKO10_08155"
     CDS             complement(473..967)
                     /locus_tag="PKO10_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW67158.1"
                     /translation="MIILNDSILSYEKRTELLFEIFKENKIIQRIFATFLIQSNREFF
                     FSNIDMFDCEKLIDILWYQKKEDIDFDKIKNNKFLNELYSAKNYINSVSNSSDFDLNI
                     LILINNHQKDIKTNLTFEFICKSCKHSSPFYDARCPNCHSILSLEVKHHLTKAYELSN
                     QSLQ"
BASE COUNT          350 a          141 c          114 g          441 t
ORIGIN      
        1 ttcaagtaga tttaaaatga taggttttag tgtttctaaa ccactctcta gatatattgc
       61 gcccattatt gcttcaaatg catctgaaag tatagaagat tttgttcgtc ctttgtttct
      121 ctcttcagcg tttgagataa atatataatc tcctaaattt atctcatttg ctaactttgt
      181 aaatccagtt tcatttacca aactagctct tattttggat agttcaccct catttgattt
      241 tggaaatttt gtatataaat actctcctac aattaagttt aaaacagcat cacctagaaa
      301 ctctaatctt tcattgttgt atggtttttt gaaactttta tgtgtaagtg cttcgattat
      361 caggttttta tttttaaact gataatccaa acacttttca agttttgaat aatcgctcaa
      421 tttttatcct ttgttttaaa attataaaag tataacacaa atatatttta ttttattgca
      481 gtgattgatt tgaaagctca taagcttttg ttaggtgatg ctttacctct aaacttaaaa
      541 tagagtgaca atttggacat ctagcatcat aaaaagggct agagtgttta caagatttac
      601 aaataaattc aaatgtaaga tttgttttta tatctttttg atggttgtta attaaaatta
      661 aaatattcaa atcaaaatca ctactatttg aaacactatt tatatagttt tttgcactat
      721 aaagttcatt taagaattta ttatttttaa ttttatcaaa atcaatatct tctttttttt
      781 gataccataa aatatcaatt aatttttcac aatcaaacat atctatattt gagaagaaga
      841 attctctatt tgattgaatt aagaatgtcg caaaaattct ttgaataatt ttattttctt
      901 taaaaatttc aaataaaagc tcagttcttt tttcataaga taaaattgaa tcatttaata
      961 taatcaaagt gtcaaaatat actttttcta tacttacatc aacatttagc tcttctaatg
     1021 cttgtgtaat ctattttgct ttttta
//
LOCUS       DAOQAN010000590         1046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_234363_length_1046_cov_2.253280, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000590 DAOQAN010000000
VERSION     DAOQAN010000590.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_234363_length_1046_cov_2.253280"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="PKO10_08160"
     CDS             <1..182
                     /locus_tag="PKO10_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW67159.1"
                     /translation="YITINIYFGINDGQIAIAFVFCFMMLILLAIDLNDKFKREFNRY
                     KKYLKIKRIRSYKGL"
     gene            complement(652..>1046)
                     /locus_tag="PKO10_08165"
     CDS             complement(652..>1046)
                     /locus_tag="PKO10_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW67160.1"
                     /translation="GVALIKPEQLLGIKIDKVTQVEKGIIYTKEKGGKQGEVLVSLET
                     YAELENYLSLNSKFKINRQDYYNDIKQAALISKEVSEGSHGLRWNFAKRRMFEYGKAD
                     YSYSDSLQEVSYEMKHNRASITEHYLRG"
BASE COUNT          388 a          132 c          113 g          413 t
ORIGIN      
        1 tttatattac aataaacata tattttggaa taaatgatgg acaaatagct atagcttttg
       61 tattttgttt tatgatgtta atacttctag caattgactt aaatgataaa tttaaacgag
      121 agtttaacag atataaaaaa tatctaaaaa ttaaaagaat cagatcatat aaaggactat
      181 aaaatgcaat tagaatttaa tacagtaaca attatcctac ttacaatgtt ggtaatcgga
      241 ataattttag cagttggcaa atttgattat gaaaaagata aaaatttaaa ataaaagtaa
      301 ttttaatatt ttataaaatc cctgtttatt tataagtaat agggatttat tatttttaga
      361 gattaaaaaa ttacttagtg ctttgatttt cgtttttctt ttttacttta aaagatagat
      421 aaagtacaaa aaatgcaaac agtgcgacta atgaaaatct taactcttcc aaattttctc
      481 ctagtttatt tatcaataaa ttttctgtgt taattatagc aaagctatca aatattacaa
      541 aatctttata tcatcaaaca aaaatatctt taaaacaaat tttaaatttt atttgtaaaa
      601 tcaaaggtta atatttttat aatataagaa cttttttaaa gaaaataaaa atcatcctct
      661 caaataatgt tcagtaatac tagctctatt atgcttcatc tcatagctaa cttcttgtag
      721 tgaatcagaa taagagtagt cagcttttcc atactcaaac attcttcttt ttgcaaagtt
      781 ccatctaaga ccatgacttc cttcagaaac ttcttttgaa ataagtgctg cttgttttat
      841 atcgttataa taatcttgtc gattgatttt aaattttgaa tttaatgata gataattttc
      901 taattcagca taagtttcaa gtgatactaa gacttcacct tgctttccac ctttttcctt
      961 tgtatagata attccttttt caacttgtgt tactttatca attttaattc caagaagttg
     1021 ttcaggtttt attaatgcaa ctcctt
//
LOCUS       DAOQAN010000591         1045 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_234728_length_1045_cov_2.163636, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000591 DAOQAN010000000
VERSION     DAOQAN010000591.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_234728_length_1045_cov_2.163636"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..981
                     /locus_tag="PKO10_08170"
     CDS             <1..981
                     /locus_tag="PKO10_08170"
                     /inference="COORDINATES: protein motif:HMM:NF019348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNW67161.1"
                     /translation="HEIKDCYPIFITEKPEVGDIAFVTKRSKFYMVANKDVLKAIKDS
                     GFGATYYTLKYNNRKIEVLKVKRFACGTLDKDISEVPYHVNIEYIDQDLTYDVKKIAD
                     HYNIATTRIIAKEKELIEEIKETLDHPLNIEKSEWLMLVADAGAGKTQISIEYANQKN
                     TDYILQQGHAQLTVDDLLGYKSITDGTYFPSLLRDAVENGKIFILDEIDACNPNTLLA
                     LNSLKNKRFQFPDKIVDIHPDFRLIATANTLEYSDVYNGRSKLDKATITRFKIIKCDL
                     EPHHLAIRYGLDYVKDIQNIDRLTPREIERIVVEKKIAEEKNGKTEDLSA"
BASE COUNT          413 a          144 c          176 g          312 t
ORIGIN      
        1 catgaaatta aagattgcta tccaattttc ataactgaaa agccagaagt tggtgacatt
       61 gcgtttgtta ctaaaagatc aaaattctac atggtagcaa acaaagatgt tcttaaagca
      121 ataaaagata gtggttttgg tgctacatac tatactttga aatataacaa cagaaaaatt
      181 gaagtgttaa aagttaaaag atttgcttgc ggtacattag ataaagatat atcagaagtt
      241 ccttatcatg taaatattga atatattgat caagatttga catatgatgt taaaaaaatt
      301 gcagaccact ataatatagc tacaactaga ataattgcaa aagaaaaaga gcttattgaa
      361 gaaataaaag aaactcttga ccatccgtta aatattgaaa aaagcgaatg gctaatgctt
      421 gtagctgatg ctggagcagg taaaactcaa atatctattg aatatgcaaa tcaaaaaaat
      481 acagattata tcctgcaaca aggtcacgct caattaactg ttgacgacct gctaggttat
      541 aaatctatca cagatggaac atatttccct tctctgctaa gagatgctgt tgaaaatggt
      601 aaaatattca ttcttgatga aattgatgct tgtaatccaa atacacttct ggctctgaat
      661 tctttgaaaa ataaaaggtt tcaattccca gataaaattg ttgatataca tccagatttt
      721 agacttattg caactgcaaa tactcttgaa tattcagatg tttacaatgg taggtctaag
      781 cttgataaag ctacaattac aagatttaag ataattaaat gtgaccttga gcctcatcat
      841 ttagcaataa gatatggtct tgactatgta aaagatattc aaaacataga tagattaact
      901 cctagagaaa tcgagagaat tgttgttgag aaaaaaattg ctgaagaaaa aaatggaaaa
      961 acagaagatt taagtgctta aacacttaag caatctgtat aaatgtgcag agccagagag
     1021 atagctttgg tttctgcact agaaa
//
LOCUS       DAOQAN010000592         1044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_235206_length_1044_cov_1.624874, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000592 DAOQAN010000000
VERSION     DAOQAN010000592.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_235206_length_1044_cov_1.624874"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="PKO10_08175"
     CDS             complement(<1..738)
                     /locus_tag="PKO10_08175"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HNW67162.1"
                     /translation="MSKIIQKQNDSTKYNRINQIYEMLKNNVHGFTIAELANELDVST
                     KTIQRDLYEVLSDLGAIKEGRTWKIDPKLANDDLNTKERLILGILDEMAKSAGNIFYS
                     KAHSLLSQITQQLDHPIFTNVNGEYLEDKTVALFEQIEKAIKDKNEIKFDYEKYNFHV
                     KPLKLAFFDGFWYLLAFHIKKNKEEFKKYHLKTIKKVEVLSTKFEIPSLVEERLKFAN
                     SVWFNLDEQYRVRLFIDKQIRKYFERKP"
     gene            complement(817..>1044)
                     /locus_tag="PKO10_08180"
     CDS             complement(817..>1044)
                     /locus_tag="PKO10_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW67163.1"
                     /translation="DQLLGMIPGLGLKKEDKEVVKDSYFFARYIQILCTTNYYKYYLD
                     NYFHIYASIEDELQSRFWSSFIVYIKKIFKI"
BASE COUNT          347 a          159 c           99 g          439 t
ORIGIN      
        1 aggttttctt tcaaaatatt ttctaatttg tttatctata aacaatctta ctctatattg
       61 ttcatccaaa ttaaaccaaa cactatttgc aaattttaat ctctcttcaa ctaaagaagg
      121 aatttcaaat tttgtactaa gcacttccac tttttttata gtttttaaat gatatttttt
      181 aaattcttct ttattttttt tgatatgaaa tgcaagtaaa taccaaaaac catcaaagaa
      241 agctaattta agaggcttta catgaaaatt atatttttca taatcaaatt tgatttcatt
      301 tttatcttta atagcttttt ctatttgctc aaacaaagca acagttttat cttcaagata
      361 ttcaccattt acatttgtaa atattgggtg atctagttgt tgagtaattt gagataaaag
      421 agagtgagct tttgagtaaa atatatttcc agcacttttt gccatctcat ctaaaatacc
      481 aagaatgagt ctttcttttg tatttaaatc atcatttgca agttttgggt caattttcca
      541 agtccttccc tcttttatag ctcctaaatc gcttaatact tcatataaat ccctttgtat
      601 tgtttttgta ctaacatcta actcatttgc taattcagca attgtaaatc cgtgaacatt
      661 atttttcaac atttcataaa tttgatttat tctattatat tttgtactat cattttgttt
      721 ttgaataatt tttgacatca tttccctttg tgtaaaatag tttttatttt acataacatt
      781 tattaaatcc ttatataatt tagacactat tttgtcctat attttaaaaa tctttttaat
      841 gtaaactata aaagaagacc aaaatctaga ttgcaactca tcttcaatag atgcataaat
      901 atgaaaatag ttatccaaat aatatttata atagtttgta gtacaaagta tttgaatata
      961 tcttgcaaaa aaataactat cttttaccac ttctttatct tcttttttta agcccaatcc
     1021 cggaatcatc cccaagagtt gatc
//
LOCUS       DAOQAN010000593         1042 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_235764_length_1042_cov_3.114488, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000593 DAOQAN010000000
VERSION     DAOQAN010000593.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_235764_length_1042_cov_3.114488"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="PKO10_08185"
     CDS             complement(<1..429)
                     /locus_tag="PKO10_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004318066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HNW67164.1"
                     /translation="MCAIVGIYGNDNAARLASIALFAMQHRGQEATGISSSCDGKIYT
                     KKDRGLVSEVFNEEALTYLKGNMAIGHNRYATAGSDSVLDAQPVYAKYKLGQISIVHN
                     GNLINKDEVRNDLIDKGAIFQTGMDTENLIHLIAKNTKDHL"
     gene            complement(444..>1042)
                     /gene="dapB"
                     /locus_tag="PKO10_08190"
     CDS             complement(444..>1042)
                     /gene="dapB"
                     /locus_tag="PKO10_08190"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HNW67165.1"
                     /translation="DVIIDFSSPVATEELLTAVIEGGKRKALVIATTGLSKHQQNLLL
                     EASKLVPILYATNMSLGVAVLNKLVALASKTLRDFDIEIVEQHHKHKVDAPSGTALTL
                     AEHAASARDLNLDEVRISGRDGQIGARTKDEIAVMALRGGDIVGRHTVGLYNDGEFLE
                     LNHTATARNTFSKGALKVAKWLVKKDANLYSINDALGL"
BASE COUNT          320 a          204 c          130 g          388 t
ORIGIN      
        1 tagatgatct tttgtatttt ttgctattag atgaataaga ttttcagtat ccattcctgt
       61 ttgaaaaata gcacctttat ctatcaaatc atttcttact tcatctttat ttataagatt
      121 accattatga acgattgata tttgacctaa tttatatttt gcataaacag gttgagcatc
      181 taaaacagaa tcacttccag ctgttgcata acgattatga ccaattgcca tatttccttt
      241 taagtaagtt aatgcttctt cattaaatac ttcagaaacc aaacctctat ctttttttgt
      301 atatatttta ccatcacaag atgatgaaat tccagttgcc tcttgacctc tatgttgcat
      361 tgcaaaaaga gctattgaag ctaatcttgc agcattatca tttccataaa ttcctactat
      421 tgcacacatt gttgatttcc ttattataga cctaaagcat cattgattga gtaaagattt
      481 gcatcttttt ttacaagcca ttttgcaact tttaacgcac cttttgaaaa agtatttcta
      541 gcagttgctg tatgatttaa ctctaaaaat tctccatcat tatataaacc aactgtatgc
      601 cttccaacta tatctccacc tcttaaagcc ataactgcaa tttcatcctt agttctagct
      661 ccaatttgac catctcgacc agaaattcta acctcatcta aattcaaatc tcttgcactt
      721 gctgcatgtt cagcaagtgt tagtgcagtt cctgatggag catctacctt atgtttatga
      781 tgttgttcaa caatttcaat atcaaaatct cttaatgttt tagaagcaag agctacaagt
      841 ttatttaaaa ctgcaactcc taaactcata tttgtagcat ataaaatagg tacaagtttg
      901 ctagcttcta gaagtaagtt ttgctggtgt ttacttaatc cagtagttgc aattactaat
      961 gcttttcttt ttccaccttc aataacagca gttaaaagct cttccgttgc aactggactt
     1021 gagaaatcaa taattacatc gc
//
LOCUS       DAOQAN010000594         1039 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_236916_length_1039_cov_2.727642, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000594 DAOQAN010000000
VERSION     DAOQAN010000594.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_236916_length_1039_cov_2.727642"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(96..>1039)
                     /gene="truD"
                     /locus_tag="PKO10_08195"
     CDS             complement(96..>1039)
                     /gene="truD"
                     /locus_tag="PKO10_08195"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014473672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HNW67166.1"
                     /translation="ILKLRKKDLATWDAIEILANYLKCSTREFGYAGLKDKNAMTVQH
                     ISIHRKYEDALKSFSHENIKILETTYHNNKIKIGHLKGNNFFIRLKRVGLVEKQKIEE
                     AFSKIAVFGMPNYFGFQRFGIDGENYKKGKQIVEGTLKERNRNLKQMFINAYQSYLFN
                     SWLSKRIEISKLIEAFEPKEICEKLNLPLDEVKRAKKQPHPFKILTGDLLSHYPFGKI
                     FIIDDLETESQKFFEKDRVPTGLLCGKRVKKSEGLAYGIEKDFEVQMSEDGARRFAFV
                     FPENLQSNYKEDKNHMELSFYLPKGSYATELIAEILH"
BASE COUNT          328 a          185 c          102 g          424 t
ORIGIN      
        1 aacttcgcat aactcagtaa ttagctcttc gtatctcatt tcttatcccc cttgtgtagt
       61 ataaaattga tactagcaaa aaagcactta aaaaattagt gtaaaatctc cgcgattaac
      121 tcagttgcat atgaaccttt aggaagataa aaacttagtt ccatatgatt tttatcctct
      181 ttataattac tttgtaaatt ttctggaaaa acaaaagcaa atcttctagc tccatcttca
      241 ctcatttgta cttcaaaatc tttttcaatc ccatatgcaa gaccttcaga tttttttact
      301 ctttttccac ataaaagacc tgttggcact ctatcttttt caaagaattt ttgactttca
      361 gtttctaaat catctattat aaaaatcttc ccaaatggat aatgacttaa taaatcacca
      421 gttaatattt taaaaggatg tggttgtttt tttgctcttt ttacctcatc aagtggaaga
      481 tttagttttt cacaaatctc ttttggctca aaagcttcaa ttaactttga tatttctatt
      541 ctttttgata accatgaatt aaaaagatag ctttgataag catttataaa catctgcttt
      601 aaatttctat ttctctcttt tagagttcct tcaactatct gcttcccttt tttataattc
      661 tcaccatcta ttccaaatct ttggaaacca aaataatttg gcattccaaa aactgcaatt
      721 tttgaaaatg cctcttctat tttttgtttt tcaacaagtc caactctttt taatcttata
      781 aaaaagttat ttccttttaa atgccctatt tttatcttat tgttatgata agttgtttca
      841 agaattttaa tattttcatg actaaaactt ttcaaagcat cttcatattt tctatgtata
      901 gagatatgtt gaacagtcat tgcattttta tcttttaagc cagcatatcc aaactctcta
      961 gtactacatt ttagataatt tgctaaaatt tcaatagcat cccaagtagc taaatctttt
     1021 tttctaagtt ttaaaataa
//
LOCUS       DAOQAN010000595         1039 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_236926_length_1039_cov_2.632114, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000595 DAOQAN010000000
VERSION     DAOQAN010000595.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_236926_length_1039_cov_2.632114"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            110..295
                     /locus_tag="PKO10_08200"
     CDS             110..295
                     /locus_tag="PKO10_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW67167.1"
                     /translation="MKNYILIILFLIIPSIILFFSNINDSKEAAIFLFIGGLVVSFLN
                     YKKDKDERVMRFLNKWL"
     gene            complement(351..602)
                     /locus_tag="PKO10_08205"
     CDS             complement(351..602)
                     /locus_tag="PKO10_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002778131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNW67168.1"
                     /translation="MEVDFSNSSKEEIEFFYKKISKNIKKYRLEKGLSQEKLALDIGV
                     KSIAFYSNCESNRYQKHFNLEHLYKISKSLNINIEDLFK"
     gene            706..>1039
                     /locus_tag="PKO10_08210"
     CDS             706..>1039
                     /locus_tag="PKO10_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW67169.1"
                     /translation="MKRILLLSSSVALATLLFTGCGIKTTEYNPSADNVQTLRDFKDL
                     KLNVSNFTSTNKGESSVLCRLAETVSTPKGEPFSTYIENALLSELKMAGNYDKNSNIN
                     LSGNINKVY"
BASE COUNT          427 a          102 c          114 g          396 t
ORIGIN      
        1 taataaaata attgttacat ttaaatctaa ttaattaaga aaataatctt taatactaaa
       61 atataacaaa gcttatactt gctataaaaa tcaactttaa ggataaaact tgaaaaatta
      121 tatacttata atactatttt taatcattcc atcaataata ctatttttta gtaatataaa
      181 tgactcaaaa gaagcagcta tatttttatt tattggtgga ttagttgtaa gctttttaaa
      241 ttataaaaaa gataaagatg aaagagtgat gagattttta aataaatggc tttaaagatt
      301 atgttcaagt gcttttataa aaatattttg aaaaattaat aaataataat ttatttaaat
      361 agatcttcaa tatttatatt taaagatttt gatattttgt ataaatgctc taaattaaag
      421 tgtttttgat atctattact ttcacaattt gaataaaatg caattgattt aacaccaata
      481 tcaagagcta atttttcttg agacaatcct ttctccaatc tatatttttt aatattttta
      541 gatatttttt tataaaaaaa ctcaatctct tctttagaac tgttactaaa atctacttcc
      601 atatatattt atcctatagg ttaaaattat tataaacttt attaagatag aatcatttag
      661 tctttatcct atgggataaa attaataaaa taaaaaaggg aaaaaatgaa aagaattcta
      721 ttattaagtt caagtgttgc attagcaact ttactgttta caggttgtgg tataaaaact
      781 acagagtata atccatctgc tgataatgta caaacattaa gagattttaa agatcttaaa
      841 ttaaatgttt caaattttac ttctacaaat aaaggtgaaa gtagtgtttt gtgtagatta
      901 gcagaaacag tatctactcc aaaaggtgaa ccattttcta catatattga aaatgcactt
      961 ttaagtgaac ttaaaatggc tggaaattat gataaaaatt caaatataaa cttaagtggt
     1021 aatataaata aagtatatg
//
LOCUS       DAOQAN010000596         1038 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_237471_length_1038_cov_1.621567, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000596 DAOQAN010000000
VERSION     DAOQAN010000596.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_237471_length_1038_cov_1.621567"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            54..698
                     /locus_tag="PKO10_08215"
     CDS             54..698
                     /locus_tag="PKO10_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008336038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HNW67170.1"
                     /translation="MFIVAKGRKQAKITRENEKVQVYSLFQRVVLLLNIVIMIYLFIT
                     GFSITFGNWTGGGYIPRLMRATHEVVGVAWIPVWFIVTVIAFKDHKYFVRPSSKIWHK
                     FFLRGKYEHMNRINYYMYVAFGFLLVLSGFTIWYMFPDAATHAQTIQIKRFILFIHFM
                     GSAIISFFTFETVYSYFVSVKGYIPGVITGKLPIEYLEQLRPDVLEEDKDLLKR"
     gene            770..>1038
                     /locus_tag="PKO10_08220"
     CDS             770..>1038
                     /locus_tag="PKO10_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008338679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HNW67171.1"
                     /translation="MDNSKYLKTVIIDKLIENEANMVEDVTIEEARLNLYLNGEKAIS
                     MMTIPKDQDAHAIGFLMSENVISSIADIEELTVSADGLRVDVKAK"
BASE COUNT          382 a          119 c          182 g          355 t
ORIGIN      
        1 accacttaca aaactaaatg tttcggcagg tgtaattttg tctgtttaca ataatgttta
       61 ttgttgcaaa aggtagaaag caagctaaaa ttacaagaga aaatgagaaa gttcaagttt
      121 attcactttt tcaaagagtt gttcttttat taaatattgt aattatgatt tatctgttta
      181 taactggttt ttcaataaca tttggaaact ggacaggtgg aggatatatt cctagactta
      241 tgagagctac acatgaagtt gttggtgttg cttggattcc agtttggttt attgttacag
      301 ttattgcatt taaagatcac aaatactttg taagacctag ttctaaaata tggcataaat
      361 tctttttaag aggaaaatat gagcatatga atagaatcaa ctactatatg tatgttgcat
      421 ttggtttttt acttgtatta agtggtttta caatatggta tatgtttcca gatgcagcaa
      481 cgcatgcaca aacaatacag ataaaaagat ttattttatt tatccatttt atgggtagtg
      541 caattatctc tttctttaca tttgaaacag tatattcata ctttgtttca gtaaaaggtt
      601 atattccagg tgtgataact gggaaattac caattgagta cttagaacaa ctaagaccag
      661 atgtattaga agaagataaa gatttattaa aaagatagga aaagctttag ggctttttct
      721 atttaaaaaa agattttaaa tatgcaaatt ttgcatatta aggaaaacta tggataatag
      781 caaatattta aaaacagtaa ttattgacaa acttatagaa aatgaagcca atatggttga
      841 agacgtgaca attgaagaag cacgtctaaa cctatattta aacggagaaa aagctatatc
      901 tatgatgaca ataccaaaag accaagatgc acatgcaatt ggttttttaa tgagtgagaa
      961 tgtaatatct tctattgctg acatagaaga gcttacagtt agtgctgatg gattaagagt
     1021 agatgtaaaa gcaaaaat
//
LOCUS       DAOQAN010000597         1036 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_238054_length_1036_cov_2.548420, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000597 DAOQAN010000000
VERSION     DAOQAN010000597.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_238054_length_1036_cov_2.548420"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..178
                     /locus_tag="PKO10_08225"
     CDS             <1..178
                     /locus_tag="PKO10_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014469246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglycerate mutase
                     (2,3-diphosphoglycerate-independent)"
                     /protein_id="HNW67172.1"
                     /translation="MRDENGNTLTNHTVGDVYCFVIASNITKVKEGSLNNIAPTVLKL
                     MGLDIPKEMDEPLI"
     gene            175..864
                     /locus_tag="PKO10_08230"
     CDS             175..864
                     /locus_tag="PKO10_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNW67173.1"
                     /translation="MIDIKELKISLNNKSLVDIAFTIKSSTALIGESGSGKSLTLKAI
                     LNLLSSSLEVKKNISSDFELSFDTIGFIPQNPFTSLSSMTKIKNQIFCSEKKKEEVFK
                     LLDLPLEILEKYPSQISGGQTQRVVIAIALSRDIKLLLLDEPTTALDFENKTNIVNIV
                     KDLKDKLNINILFVTHDIESIKDICDDIVIIKNGYVVEFGKTQEVLNNPQNEYTKKLL
                     NSSFKNKKFRN"
BASE COUNT          428 a          110 c          160 g          338 t
ORIGIN      
        1 gatgagagat gaaaatggaa atactcttac aaatcataca gttggagatg tttattgttt
       61 tgtgattgct tctaatatta caaaagtaaa agagggaagt ttaaataaca ttgcgccaac
      121 agttttaaaa cttatgggac ttgatatacc aaaagagatg gatgaaccac taatttgata
      181 gatataaaag agttaaaaat tagtttaaat aataaaagct tagttgatat tgcttttact
      241 ataaaaagtt caacagctct aataggagag agtggaagtg gaaaatcact tacactaaaa
      301 gctatcttaa atctcctatc tagttcacta gaagtaaaga aaaatataag tagtgatttt
      361 gaattgagtt ttgatacgat tgggtttatt cctcaaaatc cttttacatc attatcatct
      421 atgacaaaga taaaaaatca gattttctgt agtgaaaaga aaaaagaaga ggtttttaaa
      481 cttttagatt tacctttaga aattttagaa aagtaccctt cacaaataag tggtggacaa
      541 acgcaaagag ttgtaattgc aatagctcta agtcgtgata taaaacttct tttacttgat
      601 gaaccaacaa ctgcacttga ttttgaaaat aaaacaaata ttgtaaatat tgtaaaagat
      661 ttaaaagata agctaaatat taatatactt tttgtaactc acgatattga atcaataaaa
      721 gatatttgtg atgatattgt tattataaaa aatggatatg ttgttgaatt tgggaaaact
      781 caagaagttt taaataaccc acaaaatgaa tatacgaaaa agcttttaaa ctcaagcttt
      841 aaaaacaaaa aatttaggaa ttaaaagatg ttaaaatttt taacaaaagc gataatattt
      901 ttaggagtaa ttgttggagc tattactttt gtatatctat acgatttata tcaagagata
      961 aaagatgata ttgatagtgt tgtaaactac aatccaaaac aaagtacaca attttttgat
     1021 aaaaatggga aacttt
//
LOCUS       DAOQAN010000598         1034 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_238803_length_1034_cov_2.457610, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000598 DAOQAN010000000
VERSION     DAOQAN010000598.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_238803_length_1034_cov_2.457610"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..906
                     /locus_tag="PKO10_08235"
     CDS             <1..906
                     /locus_tag="PKO10_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014475399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HNW67174.1"
                     /translation="EGIGRPSTYAPTISTIQAREYVVKTEDKKLAPTPTGEIVNSFLT
                     DHFSNIIDLGFTARIEEQFDDIAEGKKAWVDVMKSFYGDFKKTIKDKEENISKSDYSQ
                     VRELGIDPKSGKPVSARVGRFGPFVQIGTKDDEEKPKFVAIPEKLNMDTITLEEALFL
                     FNLPRVVGVDDSGNEIKANIGRFGPYLQVKTKYYSLKTDDPYTVDEQRAREIISEIDE
                     AKSKALIKDFEKEKIQILNGQYGAYIKQGRKNFKIPKTKVAEELTLEECLEIIEKDSK
                     GTKKPARKTAIKTTTKKVSAKAKKE"
     gene            907..>1034
                     /locus_tag="PKO10_08240"
     CDS             907..>1034
                     /locus_tag="PKO10_08240"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNW67175.1"
                     /translation="MKIGILSDSHHKNEYLKECIEFLKKENCEYLIHAGDICSKDG"
BASE COUNT          440 a          118 c          198 g          278 t
ORIGIN      
        1 gaaggaattg gacgaccttc tacgtatgct ccaacaattt caacaattca agcacgtgag
       61 tatgtagtaa aaacagaaga taaaaaatta gctccaacac caacaggaga gattgtaaac
      121 agttttttaa ctgatcactt ctcaaatatt attgatttag gatttacagc gcgtattgaa
      181 gagcagtttg atgatatagc agaagggaaa aaagcttggg ttgatgtaat gaaaagcttt
      241 tatggcgatt ttaaaaagac tataaaagat aaagaggaga atattagtaa atctgattat
      301 tctcaagtaa gagagcttgg aattgaccca aaatcaggaa aacctgtaag tgcaagagtt
      361 ggaagatttg gtccatttgt tcaaattggt acaaaagatg atgaagagaa accaaaattt
      421 gtggcaattc ctgaaaaact aaatatggat actattacac ttgaagaagc actattttta
      481 ttcaatcttc caagagttgt tggagttgat gatagtggaa atgagattaa agcaaatatt
      541 ggaagatttg gtccatattt acaagttaaa acaaaatatt actcactaaa aactgatgac
      601 ccttatacag ttgatgagca aagggcaaga gagattataa gtgaaattga tgaagcaaaa
      661 agtaaagcct taataaaaga ttttgaaaaa gagaaaatcc agattttaaa cggacaatat
      721 ggagcttata taaaacaagg tagaaaaaat ttcaaaattc caaaaacgaa ggttgctgaa
      781 gaattaactt tagaagagtg tttggaaata attgaaaaag atagtaaggg tactaaaaaa
      841 cctgctagaa aaacagctat caaaactaca acaaaaaaag tatctgcaaa agcaaaaaaa
      901 gagtagatga agataggaat tttatcagat agtcatcata aaaatgaata tctaaaagag
      961 tgtatagagt ttttaaaaaa agaaaattgt gaatatttaa tacatgctgg agatatttgc
     1021 tcaaaagatg gttt
//
LOCUS       DAOQAN010000599         1034 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_238807_length_1034_cov_2.438202, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000599 DAOQAN010000000
VERSION     DAOQAN010000599.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_238807_length_1034_cov_2.438202"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1034)
                     /locus_tag="PKO10_08245"
     CDS             complement(<1..>1034)
                     /locus_tag="PKO10_08245"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HNW67176.1"
                     /translation="NIYIKGNFEEERVLFDKIDFHINKIYDINFNKKFTLLKKAFFNI
                     SNFSSNFEFENITINSSKDKKDLILNITTKDFFVEHLLYGKGFINSNVDININENSKI
                     YISGFVNPNKLVSSYNIPALNISNDRDIIIVNSGDNEIKKDFFTKNFALDLKIISKEI
                     KYITKNIELKLDADLQIKKEFEEDIRIFGRVSNINGVFSQLGKTYKIENSNLQFRGLE
                     TINPILDIKANTKIDNVEIFIDITGNMENPRLNLKSNPPMNSKDILSYLIFGTKFSNS
                     SMNEQNKEAQASLFILNELSKDYVKELGIDILHFDYDPKTQYIETTVGKKIGEKNQII
                     IKNKATNGEL"
BASE COUNT          350 a          136 c           84 g          464 t
ORIGIN      
        1 taactctcca tttgtagctt tatttttaat tataatttga tttttttcac ctattttttt
       61 accaactgtt gtctctatat attgagtttt tggatcataa tcaaagtgta atatatcaat
      121 tcctaactct tttacataat cttttgataa ctcatttaat ataaataaag aagcttgtgc
      181 ctctttattt tgttcattca tcgaactatt tgaaaatttt gttccaaaaa tcaaataaga
      241 taaaatatct tttgagttca taggtggatt tgattttaag tttagtctag gattttccat
      301 attacctgta atatcaataa aaatctctac attatcaatt tttgtatttg ctttaatatc
      361 caaaatagga tttatagttt ctaaacctct aaattgtaaa tttgaattct ctattttata
      421 tgtttttcca agttgactaa aaactccatt tatatttgaa actcttccaa aaattcttat
      481 atcttcttca aactcttttt tgatttgtaa atcagcatct agttttagtt ctatattttt
      541 tgtaatatat ttaatctctt ttgagataat ttttaaatca agtgcaaaat ttttggtaaa
      601 aaaatctttt tttatctcat tatctccact atttacaata attatatctc tatcattact
      661 aatatttaaa gctggaatat tataagaact tactaattta tttggattta caaagccaga
      721 tatatagatt ttactatttt cgttaatatt aatatccaca tttgaattta taaatccttt
      781 tccatacaaa aggtgctcta caaagaaatc ttttgtagtt atatttaaaa tcaaatcttt
      841 cttatctttt gaactattta tagttatatt ttcaaactca aagtttgaag aaaaatttga
      901 tatattaaaa aaagcctttt ttaaaagtgt aaatttttta ttaaaattta tatcatatat
      961 cttatttata tgaaaatcta tcttatcaaa aagaactctc tcctcttcaa aatttccttt
     1021 tatatatata tttt
//
LOCUS       DAOQAN010000600         1034 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_238813_length_1034_cov_2.398366, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000600 DAOQAN010000000
VERSION     DAOQAN010000600.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_238813_length_1034_cov_2.398366"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="PKO10_08250"
     CDS             complement(<1..213)
                     /locus_tag="PKO10_08250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW67177.1"
                     /translation="MRFSKEELLYENILVEMLAKEEIYSTSENRIALSMLYYCEKKIL
                     DFFNIRKDNKNKKIIDNFEEYLEKKQK"
     gene            complement(253..954)
                     /locus_tag="PKO10_08255"
     CDS             complement(253..954)
                     /locus_tag="PKO10_08255"
                     /inference="COORDINATES: protein motif:HMM:NF025843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNW67178.1"
                     /translation="MMETNEKSFNLSGVLKKVLYKNDETKYTIAVLENNQKICGVYFD
                     ADIEKLVGEEILLTGNWITHSKYGVQFEFNTLQVKEHELFFFLTKIIKGFTKKSATEI
                     LEKYGEEGLIKILDENPNELLNFKGIKEKKLKSILNSWHSFKHLRELGAFLGKFGVTS
                     NLITKIYSTFSEVENLIEKIKQNPYMLINIKGIGFKRADEIAKALGIDVKSPFRIKAC
                     LNYTLREYCDNNGNS"
BASE COUNT          323 a          159 c           86 g          466 t
ORIGIN      
        1 tttttgtttt ttttctaaat actcttcaaa attatctata attttcttat ttttattatc
       61 ttttcttata ttaaaaaaat ctaatatttt cttttcacaa taatataaca ttgaaagtgc
      121 aatacgattt tcacttgtac tataaatctc ctcttttgct aacatttcaa ctaaaatatt
      181 ttcataaagt agctcctctt ttgaaaacct caaactatca tctaaaagtt tataaagatg
      241 aaatttatct atttaggagt ttccgttatt atcacaatac tctcttaaag tataatttaa
      301 acaagctttt attctaaaag gtgattttac atcgattcct aaagcttttg ctatctcatc
      361 tgctctttta aatcctattc cttttatatt tattagcata tatggatttt gttttatctt
      421 ttcaattaag ttttcaactt cactaaaagt tgagtatatt tttgttatta gatttgaagt
      481 aactccaaat ttacccaaaa aagcccctag ttctctaagg tgcttaaaag agtgccaaga
      541 gtttaaaata gattttagtt ttttctcttt aataccttta aaatttaaaa gttcatttgg
      601 attttcatct aaaatcttta ttaaaccttc ttctccatat ttttctaaaa tctctgttgc
      661 tgattttttt gtaaaacctt tgattatttt agtaaggaaa aaaaatagtt catgctcttt
      721 tacttgtaaa gtattaaact caaattgaac tccatattta ctatgagtta tccaatttcc
      781 agttaatagt atctcttctc ctacaagttt ttcaatatct gcatcaaaat agactccaca
      841 tattttttga ttattttcta aaactgcaat agtatatttt gtttcatcat ttttatataa
      901 aacctttttt aaaactccac taagattgaa gcttttttca ttagtttcca tcattatact
      961 tatctttttt atgcttatct tttttatatg aacttttatt tttctctttt tgaagtaaat
     1021 ttgcctcttt taaa
//
LOCUS       DAOQAN010000601         1033 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_239201_length_1033_cov_2.484663, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000601 DAOQAN010000000
VERSION     DAOQAN010000601.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_239201_length_1033_cov_2.484663"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="PKO10_08260"
     CDS             <1..467
                     /locus_tag="PKO10_08260"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HNW67179.1"
                     /translation="KFIGSEYDYLKTVSDLKYIKTFDNLTASFKTKIGTLDRDLPIFK
                     HFFAGGAYSNRGYAYEKVGVKDSDDNPYGGLSMIDSSLEFEYNIYKNIGIVTFFDSTM
                     LSLESNNFNDKFYNSFGFGARYYTPIGPFRVDFGFPKDDGGFVFHIGIGQVF"
     gene            464..>1033
                     /locus_tag="PKO10_08265"
     CDS             464..>1033
                     /locus_tag="PKO10_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW67180.1"
                     /translation="MIILRALKYLFFTIISLCLVTFLYLITSKDVTQKIFNYLSVEFP
                     IKYSKVDGSIYEGIKIYDLNYDDMIKAKTVYLKPNIISLLVKEINISLLKVENITIED
                     KLISFIKESSETQNENSSFEFPFSLVIKNLELSMYDFSYENQKIDEIFLNAKNINSNL
                     KDKISAILFAKIKSNLVNLEANMQIENNIY"
BASE COUNT          408 a          100 c          144 g          381 t
ORIGIN      
        1 caaaatttat aggaagtgag tatgattatt taaaaactgt ttctgattta aaatatatta
       61 aaacttttga taatcttact gcttctttta aaacaaaaat aggtactctt gatagagatt
      121 tgcctatttt taaacacttt tttgctggag gtgcatatag taatagagga tatgcttacg
      181 aaaaagttgg tgtaaaagat agtgatgaca atccatatgg tgggcttagt atgatagata
      241 gtagtttgga atttgagtat aatatttata aaaatattgg tattgttact ttttttgact
      301 ctacaatgct tagtttagaa tcaaataatt ttaatgataa attctacaac tcttttggtt
      361 ttggtgctag atattatact ccaattggtc catttagagt tgattttggt tttccaaaag
      421 atgatggtgg atttgtattt catataggaa tagggcaagt attttgataa ttttaagagc
      481 tttaaaatat ctatttttta caataatatc tttatgttta gtaacatttt tatatcttat
      541 tacatcaaaa gatgtaacac aaaaaatatt taactatctt agtgttgaat ttcctataaa
      601 atatagcaaa gtagatggaa gtatctatga aggtattaaa atatatgatt taaactacga
      661 tgatatgata aaagcaaaaa ctgtatatct aaaaccaaat attatttcgc ttttagtaaa
      721 agagataaat atatctcttt taaaagtaga aaatataaca atagaagata aattaatctc
      781 attcataaaa gagagtagtg aaactcaaaa tgaaaatagt agttttgaat ttcctttttc
      841 tttagtcata aaaaatcttg aactctcaat gtatgatttt tcatacgaaa atcaaaaaat
      901 agatgagata tttttaaatg ctaaaaatat aaactcaaat ctaaaagata aaataagtgc
      961 cattctattt gcaaaaatca aatcaaattt agtaaatctt gaagcaaata tgcaaataga
     1021 aaataatata tat
//
LOCUS       DAOQAN010000602         1033 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_239289_length_1033_cov_2.088957, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000602 DAOQAN010000000
VERSION     DAOQAN010000602.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_239289_length_1033_cov_2.088957"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="PKO10_08270"
     CDS             complement(<1..427)
                     /locus_tag="PKO10_08270"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HNW67181.1"
                     /translation="MKIIYTKDKEFKTEFENILKRAKSDIKGVSKIVENIIDEIVEDG
                     NEALKRHIEKFDKWIVKSDDELLISQKDMKKAYETLDESLKKSLHIAYERIKIYHEKQ
                     LPKSWIDFEENGTILGQKVTAVDRAGLYIPGGKAAYPSSL"
     gene            complement(424..876)
                     /locus_tag="PKO10_08275"
     CDS             complement(424..876)
                     /locus_tag="PKO10_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013134452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HNW67182.1"
                     /translation="MVGINSHFQYVIDTDDLIESMENRAIKKIFAVEGEPYFRNLEKK
                     TALWLENSVENTIISTGGGFYRQENLKNIGTVIYLKSSFDGILKRIKKAPNAKNKLKK
                     RPLLQNKKEAMKLYDTRVKEYERVADIIVDVENRDLKLIVKEILGQIK"
BASE COUNT          283 a          206 c          104 g          440 t
ORIGIN      
        1 ataaagagct tggatatgca gcttttccac ctggtatata aagtccagct ctatcaacag
       61 cagttacttt ttgtcccaaa attgtcccat tttcttcaaa atcaatccaa gattttggaa
      121 gctgtttttc gtggtaaatt tttattcttt catacgctat atgaagtgat ttttttaaac
      181 tctcatcaag agtttcataa gcttttttca tatctttttg agatattaaa agttcatcat
      241 cacttttaac aatccatttg tcaaattttt caatatgtct ttttaaagcc tcatttccat
      301 cttcaacaat ctcatcaata atattttcaa ctatttttga aacacctttt atatcacttt
      361 ttgctctttt taaaatattt tcaaactctg ttttaaactc tttatctttt gtatatatta
      421 ttttcatttt atctgtccta aaatctcttt tactataagt ttcaaatctc tattttcaac
      481 atcaacaata atatctgcaa ctctttcata ctctttaact cttgtatcat aaagtttcat
      541 agcctctttt ttattttgta aaagtggtcg cttttttaac ttattttttg catttggtgc
      601 tttttttatt ctttttaaaa ttccatcaaa tgatgactta agatatatta cagttcctat
      661 atttttaaga ttctcttgtc tataaaatcc accaccagtt gaaataattg tattttctac
      721 actattctca agccaaagag ctgtcttttt ttctaaattt ctaaaatatg gttcaccttc
      781 aacggcaaag atctttttta tagctctatt ttccatactc tcaattaaat catctgtatc
      841 tatcacatac tggaagtggg aatttattcc caccattccc gtttctttgt tttcttcatt
      901 cggaactggg acttcagtcc cagctttatt tttatatggg actaaagtcc catttccgca
      961 aacttcttct tttccaccca ttcggaagtg ggacttcagt cccacctctt ttaccatagc
     1021 tctagcaaca gtt
//
LOCUS       DAOQAN010000603         1030 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_240291_length_1030_cov_3.055385, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000603 DAOQAN010000000
VERSION     DAOQAN010000603.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_240291_length_1030_cov_3.055385"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="PKO10_08280"
     CDS             complement(<1..214)
                     /locus_tag="PKO10_08280"
                     /inference="COORDINATES: protein motif:HMM:NF012274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HNW67183.1"
                     /translation="MSIKIVLNGAGRIGKSILKQFLNDERFEIVAINEINPSVENIVY
                     SINYDSTYGKYEDKFKIVENNYIQNST"
     gene            complement(230..421)
                     /locus_tag="PKO10_08285"
     CDS             complement(230..421)
                     /locus_tag="PKO10_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001014314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YwbE family protein"
                     /protein_id="HNW67184.1"
                     /translation="MDPKKRFDVKVGLKVNIVLKADQRTGKLTQGVVKDILTNSPTHP
                     HGIKVRLQNGDVGRVQQIL"
     gene            complement(478..>1030)
                     /locus_tag="PKO10_08290"
     CDS             complement(478..>1030)
                     /locus_tag="PKO10_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HNW67185.1"
                     /translation="VEAKTGFEMHPHENMEIISYIVDGEITHKDSMGNSETLKRGEVQ
                     YLSAGDGIYHSEKNESDKILRLLQIWVFPPQKGLPRLYGSKRYTKEQRDNKLLHIVSS
                     QDGNCDIKIYQDVNIYVSEFDKPLEYEIKENRQIYFVQIEGSSTVNGVALDFGDAMEI
                     TDEDKIVINPISKSHILFIEMKK"
BASE COUNT          313 a          175 c          104 g          438 t
ORIGIN      
        1 atgttgagtt ttgaatatag ttattttcaa cgattttaaa tttatcttca tattttccat
       61 aagttgaatc atagtttata gagtaaacta tattttcaac agatggattt atctcattta
      121 ttgctacaat ttcaaatctt tcgtcattta aaaactgctt taaaattgat tttccaattc
      181 taccagctcc atttaaaaca atttttatac tcataattta atcttttttt taaagtattt
      241 gctgaacacg accaacatct ccattttgta atcttacttt tattccatgt ggatgagttg
      301 gtgaatttgt taaaatatct tttacaactc cttgagttag tttacctgtt ctttgatctg
      361 cttttaatac aatatttact tttaaaccta cttttacatc aaaccttttt ttcggatcca
      421 tttttacttc tttccatata aaatgcaaaa ttttattgaa gtatagtaaa aaatatatta
      481 ttttttcatt tctataaata aaatatggct ttttgaaata ggatttatta ctattttatc
      541 ttcatctgta atctccatag catcgccaaa atccaatgcg actccattta cagttgaact
      601 tccttcaatt tgtacaaaat agatttgtct attctcttta atctcatatt caagtggttt
      661 atcaaattca ctaacataaa tatttacatc ctgatatatc tttatatcac aatttccatc
      721 ttgacttgaa actatatgta aaagtttatt atctctttgc tcttttgtat atctttttga
      781 accataaagt cgtggaagtc ctttttgtgg tgggaatacc caaatttgta gaagtcttaa
      841 aatcttatca ctctcatttt tttcactatg atatattcca tctccagcgc ttaagtattg
      901 aacttcacct ctttttaaag tttcactatt tcccatagag tctttatgag taatttcacc
      961 atcaacaata tatgagataa tctccatatt ttcatgagga tgcatctcaa agccagtttt
     1021 tgcttcaact
//
LOCUS       DAOQAN010000604         1028 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_241231_length_1028_cov_1.883864, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000604 DAOQAN010000000
VERSION     DAOQAN010000604.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_241231_length_1028_cov_1.883864"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /gene="pseF"
                     /locus_tag="PKO10_08295"
     CDS             complement(<1..667)
                     /gene="pseF"
                     /locus_tag="PKO10_08295"
                     /EC_number="2.7.7.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudaminic acid cytidylyltransferase"
                     /protein_id="HNW67186.1"
                     /translation="MQNSCVAIIPARGGSKRIPKKNIKLFHGKPLIAYSIEAAIKSKL
                     FSKVIVSTDDEEIAKIARSYGADVPFIRPKELSDDFTGTGAVVNHAIEFLKAQGEKID
                     FICTIYATAPFLQEKYLIEAYEKLKNSNARNAFSCTSMPFPIQRTFKITKDERCEMFW
                     PENFSKRSQDLEEAFQDAGQFYWTNLNVKSDDIIFGKDSIPIILPRYLVQDIDTLEDW
                     TRAE"
     gene            complement(657..>1028)
                     /locus_tag="PKO10_08300"
     CDS             complement(657..>1028)
                     /locus_tag="PKO10_08300"
                     /inference="COORDINATES: protein motif:HMM:NF019884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HNW67187.1"
                     /translation="YKCGFIENWGKGTINIVNECLEYGLPRPTFEYEWEAVRTTFYKR
                     VENEIIDVGVNVGVNVGVNVGVNEIYEYIKQNQPIRAKAISNHFSQITQRTIERYIKQ
                     LKDENKIEFRGIPKTGGYFAK"
BASE COUNT          308 a          188 c          115 g          417 t
ORIGIN      
        1 attctgctct agtccaatcc tcaagtgtat caatatcttg aactaaatat ctaggaagta
       61 ttataggaat actatcttta ccaaatataa tatcatctga ttttacattt aaattcgtcc
      121 aataaaactg tccagcatct tggaaagctt cttctaaatc ttgacttcgt ttagaaaaat
      181 tctctggcca aaacatttca catctttcat cttttgttat tttaaaagtt ctttgaatag
      241 gaaatggcat agatgtacaa gaaaaagcat ttcttgcatt tgaatttttt aatttttcgt
      301 aagcttcaat taaatatttc tcttgcaaaa atggagccgt tgcatagatt gtacatatga
      361 aatctatttt ttcaccttga gcttttaaaa attctatagc atgatttaca acagcacctg
      421 ttcctgtaaa atcatcactt aactcttttg gtcttataaa tggaacatct gctccataac
      481 ttctagcaat ttttgctatc tcttcatcat ctgttgaaac aataacttta gaaaaaagtt
      541 ttgatttaat agcagcttca atactataag caataagtgg ttttccatga aataatttta
      601 tatttttttt aggaattctt ttgcttccac ctcttgctgg aattattgct acacaactat
      661 tttgcaaagt atcctcctgt ttttggtatt ccacgaaatt ctattttatt ttcatcttta
      721 agttgtttta tatatctctc tattgttctt tgagttattt gtgagaaatg atttgatatt
      781 gcctttgctc ttataggttg attttgtttt atatactcat atatttcatt tacaccgaca
      841 tttacaccga catttacacc gacatttaca ccgacatcta ttatctcatt ttctactctt
      901 ttataaaaag tagttcttac agcttcccac tcatactcaa atgttggtct tggaagtcca
      961 tattctaaac actcatttac aatatttata gttccctttc cccaattttc tataaatcca
     1021 cacttata
//
LOCUS       DAOQAN010000605         1028 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_241255_length_1028_cov_1.780062, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000605 DAOQAN010000000
VERSION     DAOQAN010000605.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_241255_length_1028_cov_1.780062"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(111..434)
                     /locus_tag="PKO10_08305"
     CDS             complement(111..434)
                     /locus_tag="PKO10_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNW67188.1"
                     /translation="MKRSFIREILEAIDEETISFAGGLPSENLFPTQDLKEATLKAIE
                     NPKVYQYTISNGINELREQIALRYTNEGFATTKENILITTGSQQAMYILAKFFENKDI
                     TIEEP"
     gene            586..>1028
                     /locus_tag="PKO10_08310"
     CDS             586..>1028
                     /locus_tag="PKO10_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNW67189.1"
                     /translation="MKKSTYEKRAKIANDVMNYIYKYIDTNINIDDLSLQLNISKFHL
                     HRVFKEEFGKNIYESIKSIRLEKAANLLITNKFSTITDISNMTGYSSQTSFLRAFKQR
                     FSMTPKEWKNGGYKEYSNKIIEKISISNDLDFSNIEPIIVKMPQM"
BASE COUNT          377 a          137 c          118 g          396 t
ORIGIN      
        1 gtctttttaa aacttttctc aaactcttta atgtttactc catcatcttc tagttttaca
       61 ccttgcattt ttagaaagtt taatctaaag atattcatag ctcctaaata ttatggctct
      121 tcaatagtaa tatctttgtt ttcaaaaaac tttgccaaaa tatacatggc ttgttggctt
      181 cctgttgtaa ttaagatatt ctcttttgtt gtagcaaatc cttcatttgt gtatcttaaa
      241 gcaatttgtt ctcttaactc atttattcca ttgctaattg tgtattgata aacttttgga
      301 ttttctattg cttttaaagt tgcttctttt aaatcttgcg ttggaaatag attttcactt
      361 ggaagtccac ctgcaaatga aattgtctct tcatctattg cttcaagtat ctctctaata
      421 aatgatcttt tcataaaatc tccttttaaa tggtttccca tttaaaaaga aaccattaat
      481 atttttaaaa ttttacatca ataaaaatat ttatacttta caaaaaatgc ttttataatt
      541 atcaattatt gctaaaaata tatacattta ttgatataat actttatgaa aaaatctact
      601 tatgaaaaaa gagcaaaaat tgcaaatgat gtaatgaact acatttataa atatattgat
      661 acaaatataa atatagatga tttaagttta caattaaata ttagtaagtt tcatcttcac
      721 agagttttta aagaggagtt tggaaaaaat atttatgaaa gcattaagtc aatacgactt
      781 gaaaaagctg caaacctatt aattacaaat aagttttcaa caatcactga tatttcaaat
      841 atgactggat atagttcaca aacatctttt ttaagagcat tcaaacaaag attttctatg
      901 acaccaaaag agtggaaaaa tggtggatat aaagagtatt caaataagat tattgaaaaa
      961 atatcaatta gtaatgattt agatttttca aatattgagc ctataattgt taaaatgcca
     1021 cagatgag
//
LOCUS       DAOQAN010000606         1026 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_241919_length_1026_cov_2.269825, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000606 DAOQAN010000000
VERSION     DAOQAN010000606.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_241919_length_1026_cov_2.269825"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="PKO10_08315"
     CDS             <1..184
                     /locus_tag="PKO10_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW67190.1"
                     /translation="TAFYYNSNDGRVLVSFMAILFFLIGIKFYLEDNFKEELYRYKKY
                     LVLKRKRDNKIKVVNK"
     gene            complement(186..>1026)
                     /locus_tag="PKO10_08320"
     CDS             complement(186..>1026)
                     /locus_tag="PKO10_08320"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNW67191.1"
                     /translation="KNQIVDFQDINVHHLSNAYKRRIEGNSGKNIQAINMLSRALDSG
                     IYADYLLVDSWYAKPNFINEVKQKGIDVITRVANSNKIWQFADKCKTLESLYVQANKT
                     KASKLGNYNSIKYSYVSTTATHKTVGRVKIVFIKTKENLIPILSTNTILSDIEIINTY
                     KKRWNIEQGYKDLREYFQFGKEENRIFEALIARITLSFLAYNLTSYINRINNEPQTLG
                     NLFRDLECQLETLAISMEIFLKILENLLQSQQLVKRNKDLEQIIYMLRVYTKKQLGLM
                     CES"
BASE COUNT          321 a          144 c          160 g          401 t
ORIGIN      
        1 aactgcattt tactataatt caaatgatgg aagagtttta gtaagcttta tggcaatact
       61 tttctttctt ataggtatta aattttattt agaggataat tttaaagaag agctttatag
      121 atataaaaaa tatttagtac taaaaagaaa aagagataat aaaattaaag ttgtaaataa
      181 ataactcaac tttcgcacat aagcccaagc tgttttttag tgtaaaccct gagcatgtaa
      241 ataatctgtt ctaaatcttt atttctcttg acaagctgtt gtgattggag caaattttca
      301 aggattttga gaaatatctc cattgatatt gctaaggttt caagttggca ctctaggtct
      361 ctaaatagat tgcccaatgt ttgtggttcg ttgttgattc tgttgatata acttgtaaga
      421 ttgtatgcta aaaatgataa agtaattcta gctataagtg cttcaaagat tctattttct
      481 tctttgccaa attggaagta ttctctaagg tctttatatc cttgctcaat attccaccgt
      541 tttttatagg tattgatgat ttctatgtca gaaagtatag tattcgttga gaggattgga
      601 attaaatttt ctttggtttt aataaatacg atttttactc taccaacagt tttatgtgta
      661 gcagtggttg acacataaga gtatttgata gagttgtagt ttccaagttt tgaagcttta
      721 gttttattgg cttgaacata caagctttca agagttttac atttatctgc aaactgccaa
      781 attttatttg aattagcaac tcttgtaatc acatcaatac ccttttgttt cacttcattt
      841 ataaagtttg gttttgcata ccaactatca acaagcaagt aatcagcata tatccctgaa
      901 tctaaagctc tactcaacat atttattgct tgaatgtttt taccactgtt tccctcaatt
      961 cttcgtttgt aagcgttgct tagatgatga acattgatat cttgaaaatc aacaatttgg
     1021 tttttg
//
LOCUS       DAOQAN010000607         1025 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_242165_length_1025_cov_3.511340, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000607 DAOQAN010000000
VERSION     DAOQAN010000607.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_242165_length_1025_cov_3.511340"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="PKO10_08325"
     CDS             complement(<1..439)
                     /locus_tag="PKO10_08325"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNW67192.1"
                     /translation="MKNFVKLEDKNIKFGDCNNCFAHCCSGIFGSIFSQILKEEFEDV
                     YKNFPILFIFGSLGFVKPVILLSNGFDFCPHLKDFRCKIYENRPKVCRTYPLSPNIDN
                     FIYIDNSCPELNKADNILNFEDEIFENYQEKYINTHFEFEKLKI"
     gene            503..>1025
                     /locus_tag="PKO10_08330"
     CDS             503..>1025
                     /locus_tag="PKO10_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNW67193.1"
                     /translation="MQKKLIQIIKKAGKILKKGYYSNKDVNFKAKKDLVTKYDLAVEN
                     YLKKEFSKYFKEFNLIAEESDNSLVEFGNSIIIDPIDGTTNFVNGVPHTAISVGVYKD
                     KKPYIGVVYNPILDELYFAKHKKGAFLNGKKIKVSLQNDLQKSLLATGFPYSSGENTK
                     DLKDVIKKIENILP"
BASE COUNT          433 a          111 c          132 g          349 t
ORIGIN      
        1 caattttaag tttttcaaat tcaaagtgag tattaatata tttctcttga taattttcaa
       61 atatctcatc ttcaaaattt aagatattat ccgctttgtt taattctgga catgagttgt
      121 caatatagat aaaattatct atatttggac ttaaaggata agttctacac acttttggtc
      181 tattttcata aattttacat ctgaaatctt ttaaatgtgg acaaaaatca aaaccatttg
      241 atagtaaaat tacaggtttt acaaaaccta aagagccaaa aataaataaa ataggaaagt
      301 ttttataaac atcttcaaac tcttctttta gaatttgtga aaaaatagaa ccaaaaattc
      361 cactacaaca atgagcaaag caattattac aatcaccaaa tttaatattt ttatcttcaa
      421 gttttacaaa gtttttcatg ggtgcaattt tacatagatt ttaaaagaat tagataaaat
      481 atcaaaaaat ttaaggaaaa tcatgcaaaa gaaacttatt caaattataa aaaaagctgg
      541 aaaaatttta aaaaaaggtt attattcaaa taaagatgta aattttaaag caaaaaaaga
      601 tttagttaca aaatatgatt tagcagttga aaattatcta aaaaaagagt tttctaaata
      661 tttcaaagag tttaatctta tagctgaaga gtcagataat agtttggtag agtttgggaa
      721 ttcaataata attgatccaa tagatggaac tacaaacttt gtaaatggtg taccacatac
      781 tgcaatttct gttggagttt ataaagataa aaagccatat attggtgtag tatataatcc
      841 tattttagat gagttatatt ttgcaaaaca taaaaaaggt gcatttttaa atggtaaaaa
      901 aataaaagta agtttgcaaa atgatttaca aaaatcactt ctagccacag gttttccata
      961 tagtagtggt gaaaatacaa aagatttaaa agatgtgata aaaaaaattg agaatatctt
     1021 acctg
//
LOCUS       DAOQAN010000608         1025 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_242173_length_1025_cov_3.310309, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000608 DAOQAN010000000
VERSION     DAOQAN010000608.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_242173_length_1025_cov_3.310309"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            87..>1025
                     /locus_tag="PKO10_08335"
     CDS             87..>1025
                     /locus_tag="PKO10_08335"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HNW67194.1"
                     /translation="MKADVIVGIQWGDEGKGKIVDMLAQKYDMVCRSQGGHNAGHTIW
                     VDGVKYVLQLIPSGILNPKAINIIGNGVVVSPSNILKEMSQFGSLVGRLYISDKAHLN
                     LSFHALIDQAKERLKGEKAIGTTGKGIGPTYSEKVSRNGFRAGDLLNPSKLCDDILEY
                     FVQNRAIFDVLDIKTPTKTELLNELEDYSSKLAPYITNTTNMVWKALEENKRVLLEGA
                     QGTLLDIDHGTYPYVTSSSTVSGGACTGLGLNPKDIGDITGIVKAYCTRVGNGPFPTE
                     DFTHYGKTMGEVVKEFGAVTGRKRRCGWFDAVAVRYA"
BASE COUNT          373 a          115 c          221 g          316 t
ORIGIN      
        1 aggtggaatg tacgagactg atggaattag ctctctaggt tttgcgctat acacagataa
       61 tttattaaaa atgttagagg gataaaatga aagcagatgt aatagttgga attcaatggg
      121 gagatgaagg caagggtaag atagtagata tgcttgctca aaagtatgat atggtttgta
      181 gaagtcaagg tggacacaat gctggtcata caatttgggt tgatggtgta aaatatgtac
      241 ttcagttaat tccttcagga attttaaatc caaaagctat aaatattata ggaaatggag
      301 ttgttgtatc tccttcaaat atattaaaag agatgagcca atttggaagt ttagttggaa
      361 gattatatat atcagataag gctcatttaa atctatcatt ccatgcttta attgatcaag
      421 caaaagagag attaaaaggt gaaaaagcta ttggtacaac aggaaaagga ataggaccaa
      481 catactcaga aaaagtaagc cgtaatggtt ttagagcagg agatttatta aatccttcaa
      541 aattatgtga tgatatatta gaatattttg tacaaaatag agcaattttt gatgttttag
      601 atattaaaac tcctacaaaa actgaacttt taaatgagct tgaggattat agctctaaat
      661 tagcaccata tattacaaat actacaaata tggtttggaa agctcttgaa gagaataaaa
      721 gagttttact tgaaggtgct caaggaactc ttcttgatat tgaccatggt acatatcctt
      781 atgttacttc ttcaagtaca gttagtggtg gagcatgtac tggtttggga ttaaatccaa
      841 aagatattgg tgatataact ggaatagtaa aagcttactg tacaagagtt ggaaatggac
      901 catttccaac agaagatttc actcattatg gaaaaactat gggtgaagtt gtaaaagagt
      961 ttggtgcagt aactggaaga aaaagaagat gtggttggtt tgatgctgtt gcagttagat
     1021 atgca
//
LOCUS       DAOQAN010000609         1024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_242645_length_1024_cov_2.113519, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000609 DAOQAN010000000
VERSION     DAOQAN010000609.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_242645_length_1024_cov_2.113519"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..864)
                     /locus_tag="PKO10_08340"
     CDS             complement(<1..864)
                     /locus_tag="PKO10_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNW67195.1"
                     /translation="MVQIVNLKKSFGARVLFAEINLKLDAGKRYGLIGANGAGKTTFL
                     KILSGQEEATEGEVQIQNGKKVGVLSQNQFAFENFTLFDTVLMGNKRLYDAIKEKEEL
                     YLSPEFTDEVNNRLAELEIICCEEDPTYEYDIKVTKILEDLGFPASTQQNLMSTLTGG
                     DKVKILLAQVLYPKPDILFLDEPTNNLDIDTIGWLENQLQHHDGTMVVISHDRHFLNA
                     VCTNILDVDFKKIREFSGTYDDWYIASTLIAKQQQIDVNKKQKEKEELEKFIARFSAN
                     ASKAKQATSRQK"
BASE COUNT          316 a          178 c          117 g          413 t
ORIGIN      
        1 tttttgtcta gaagttgctt gttttgcttt tgaagcattt gcactaaatc tagcaataaa
       61 tttctcaagc tcctctttct ctttttgctt tttatttaca tctatttgtt gctgttttgc
      121 aattaaagtt gaagctatat accaatcatc ataagttcca ctaaactctc ttattttttt
      181 gaaatcaaca tctaaaatat ttgtacaaac agcatttaag aagtgtctat catgagagat
      241 tacaaccatt gttccatcgt ggtgttgaag ttgattttca agccaaccaa tagtatcaat
      301 atctaagtta tttgtaggct catctaaaaa tagaatatct ggttttggat ataaaacttg
      361 tgctaaaagt attttaactt tatcaccacc tgttaaagta ctcattaaat tttgttgagt
      421 tgaagctgga aatcctaaat cttcaagaat ttttgtaact ttaatatcat actcataagt
      481 tgggtcttct tcacaacaaa taatttcaag ctctgctaat ctattattta cttcatctgt
      541 aaattcagga ctcaaataaa gctcctcttt ctcttttata gcatcatata gtcttttatt
      601 tcccattaaa actgtatcaa aaagagtaaa attttcaaaa gcaaattgat tttgagataa
      661 aacccctact tttttaccat tttgaatttg cacttctcct tcagtagcct cttcttgtcc
      721 tgaaagaatt tttagaaatg tagtttttcc agcaccattt gcaccaatta aaccatatct
      781 tttccctgca tctagcttta gatttatctc agcaaaaaga actcttgctc cgaaagattt
      841 ttttaaatta actatttgaa ccatattaca acttctccat ttttatttaa ctattttatt
      901 tttttgtgat tttttaattt taatttggcg attatatcca aaaaataagt tttatggctt
      961 aaaagagctt agagctcttt ttcaaatgcc aatttcataa tatcatcttc gcttataata
     1021 tcgc
//
LOCUS       DAOQAN010000610         1022 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_243318_length_1022_cov_2.440538, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000610 DAOQAN010000000
VERSION     DAOQAN010000610.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_243318_length_1022_cov_2.440538"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="PKO10_08345"
     CDS             <1..347
                     /locus_tag="PKO10_08345"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNW67196.1"
                     /translation="INILNNAKDAIVLNLKDNEYGLIKIKTKKIDNFIEISILDNAKG
                     IEKDILNKIFEPYFTTKHKYQGTGLGLYMTRKIINSSMGGEITVQNKKFVHNQKKYEG
                     AEFKIKIPIKLD"
     gene            419..>1022
                     /locus_tag="PKO10_08350"
     CDS             419..>1022
                     /locus_tag="PKO10_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012857153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HNW67197.1"
                     /translation="MAKSLYETLEVSENASADEIKKAYRKLARKYHPDVNKDPKAEEK
                     FKEINAAYEVLSDPQKKQQYDQYGDSMFGGQNFSDFARNQGGGVDLDEILRQMFGGGA
                     AGFGRSNFGGGFGFDAPDLDTNAQITIPFEVAVLGGKRNISLNNDSFDIKIPEGIEDG
                     QRIRAKGKGKSYQGQRGDLILKINISPSNEYEREFDNLIKY"
BASE COUNT          437 a           98 c          184 g          303 t
ORIGIN      
        1 ttataaatat tttaaacaat gcaaaagatg ctatagtttt aaatttaaaa gacaatgaat
       61 atggtttaat aaaaataaaa acaaaaaaaa ttgataattt tatagaaatt tcgattttag
      121 acaatgcaaa aggtatcgaa aaagatattt taaataaaat atttgaacca tattttacta
      181 caaaacataa atatcaaggt actggtcttg gtctttatat gactagaaaa attataaatt
      241 ctagcatggg cggtgaaata actgttcaaa ataaaaaatt tgtacacaat cagaaaaagt
      301 atgaaggggc agagtttaaa ataaaaattc ctatcaaact tgattgactt tgactcaagg
      361 tttgtgatat aataaagtcc taataattta aaaattttaa taatatagga tttgaaaaat
      421 ggcaaaaagt ttatatgaaa cattagaagt aagtgaaaat gcatcagcag atgagataaa
      481 aaaagcttac agaaaactag caagaaaata tcatccagat gtaaataaag atccaaaagc
      541 tgaagagaaa tttaaagaga taaatgcggc ttatgaagtt ttaagtgatc cacaaaaaaa
      601 acagcaatat gaccaatatg gagactctat gtttggtggg caaaatttta gtgattttgc
      661 tagaaatcaa ggcggtggag ttgatttaga tgagatttta agacagatgt ttggtggtgg
      721 tgccgctgga tttggaagaa gtaattttgg tggcggattt ggatttgatg ctccagattt
      781 agatacaaat gcacaaatta caataccttt tgaagttgca gttttaggtg ggaaaagaaa
      841 tatctcttta aataatgatt catttgatat aaaaattcca gaaggaattg aagatggtca
      901 aagaataaga gcaaaaggaa aaggaaaatc atatcaagga caaagaggag atttaatttt
      961 aaagataaat atctctccta gcaatgaata tgaaagagag tttgataatc ttataaaata
     1021 tt
//
LOCUS       DAOQAN010000611         1021 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_243664_length_1021_cov_2.664596, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000611 DAOQAN010000000
VERSION     DAOQAN010000611.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_243664_length_1021_cov_2.664596"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="PKO10_08355"
     CDS             complement(<1..279)
                     /locus_tag="PKO10_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNW67198.1"
                     /translation="MGTITFLLVLSFLVFFHELGHFLAARFFGVKVEKFSIGFGKAIY
                     SKYWAGTTWQFAMIPLGGYVQMKGQDDSNPTLVETGNDSYNTKKPWQRI"
     gene            complement(288..830)
                     /gene="pgsA"
                     /locus_tag="PKO10_08360"
     CDS             complement(288..830)
                     /gene="pgsA"
                     /locus_tag="PKO10_08360"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HNW67199.1"
                     /translation="MSKNLNLPNILALFRIALAPLMLWFFVDRDNTIFSSWHPSWFDY
                     FAGLIFVIASVTDFFDGYIARTWDQMTKLGGILDPLADKMLVLAGFLGLMVIDRADVW
                     AVFLILSREFFITGLRVVAVAEGKNVASTMAGKVKTVAQMFAIGFLIMNWPYATTLLW
                     FAVALTLYSGFEYTRDYFKA"
     gene            complement(830..>1021)
                     /locus_tag="PKO10_08365"
     CDS             complement(830..>1021)
                     /locus_tag="PKO10_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013463484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNW67200.1"
                     /translation="IDTDALKAFTNYEEVKAKTAEYSPLNRIGQPEDLAQSCYFLCTS
                     DASWITGHTLIVDGGTTFR"
BASE COUNT          378 a          192 c          137 g          314 t
ORIGIN      
        1 aattctttgc caaggttttt ttgtattgta tgagtcattt ccagtttcta ctaaagtagg
       61 gtttgagtca tcttgacctt tcatttgtac atatccacca agaggtatca tagcaaactg
      121 ccaagttgta cctgcccaat atttactata aattgctttt ccaaatccaa ttgaaaactt
      181 ttcaaccttt acaccaaaaa atcttgctgc taaaaagtgt cctaattcat gaaaaaatac
      241 taaaaaagat aaaaccagta aaaaagtaat agtacccaaa aaaatcctta agctttgaag
      301 taatctctag tatattcaaa accagagtat agagttaaag ctacagcaaa ccataaaaga
      361 gttgttgcat atggccaatt cataattaaa aatccaatag caaacatttg agctactgtt
      421 tttacttttc cagccattgt acttgctaca tttttgcctt cagctactgc aacaactcta
      481 agacctgtta taaaaaactc tcttgaaagt attaaaaaaa cagcccatac atcagctcta
      541 tcaataacca ttaaacccaa aaaacctgca agaacaagca ttttatcagc aagtggatct
      601 aaaattccac caagttttgt catttgatcc caagttcttg caatatatcc atcaaaaaaa
      661 tctgtaacag aagcaattac aaatataagt ccagcaaaat agtcaaacca agatggatgc
      721 caagatgaga aaattgtatt gtccctatct acaaaaaacc aaagcattag cggagctaat
      781 gctattctaa atagagctaa aatatttgga agatttagat tttttgacat tatctaaaag
      841 ttgtcccacc gtcaactatt aatgtatgac ctgtaatcca agaagcatca gaagtacata
      901 aaaaataaca agattgtgct aaatcttcag gttgaccaat tctatttaat ggagagtatt
      961 ccgctgtttt tgcttttact tcttcatagt ttgtaaatgc tttaagagca tcagtgtcaa
     1021 t
//
LOCUS       DAOQAN010000612         1020 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_244257_length_1020_cov_1.699482, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000612 DAOQAN010000000
VERSION     DAOQAN010000612.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_244257_length_1020_cov_1.699482"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(170..535)
                     /locus_tag="PKO10_08370"
     CDS             complement(170..535)
                     /locus_tag="PKO10_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013459334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNW67201.1"
                     /translation="MKILVTDDSKMARKMVIKTLQDALNDKNYEVLEAQNGQESVDLY
                     KEHNPNIVFMDLTMPVMDGFEALKQIKEFNENAKVVVISADIQKQAMDKVRELGALNF
                     VKKPIDLKKMEQILNSIIS"
     gene            617..>1020
                     /locus_tag="PKO10_08375"
     CDS             617..>1020
                     /locus_tag="PKO10_08375"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014474065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNW67202.1"
                     /translation="MKKYDYIIIGSGIVGCSLFYFLKKYSNNILLIDKNSDVCLGASG
                     AAGAFLSPLLGIDNSFKTLVANALKFSTKFYEENFPQSFVKSGTIRIPKNKADEDKFN
                     NYIPYMDFPFEKKDNGYYFEIGSSVKPEILCK"
BASE COUNT          342 a          135 c          122 g          421 t
ORIGIN      
        1 ttttaaatct acagcattta ttatttgaat agttggaata ctaagttttg caaaagcgtc
       61 aaatatctca gtaattgcag cagttgcgct tccataagct acattcataa gctcttgaag
      121 gcaatctttt tcatcttctg ttaattctat attactcatt ttttaccttt taagagatta
      181 tactatttaa aatttgttcc attttcttca aatcaattgg tttttttaca aaatttagtg
      241 caccaagctc tctaacttta tccatagctt gtttttgtat atcagcagat attacaacta
      301 cttttgcatt ttcattaaac tcttttattt gttttaaagc ttcaaatcca tccattacag
      361 gcattgttaa atccataaaa actatattcg gattgtgctc tttatataaa tcaacactct
      421 cttgaccatt ttgagcttct aaaacttcat aatttttatc attcaaagca tcttgaagtg
      481 tttttattac catttttcta gccattttag aatcatctgt tactaaaatt ttcataaact
      541 accttttaaa aataattctg tattttatct acttaaattt aaaatatact ttgatttttt
      601 ttgatatact attttcatga aaaaatatga ttatataatt attggaagtg gaatagttgg
      661 atgttcttta ttttattttt taaaaaaata ttctaataac attttactta ttgataaaaa
      721 tagtgatgtt tgtttgggtg caagtggagc tgctggagct tttttatcgc ctcttttagg
      781 tattgataat agttttaaaa ctttagtagc aaatgcttta aagttttcaa caaagtttta
      841 tgaagagaat tttccacaaa gttttgtaaa atctggaaca ataagaattc caaaaaataa
      901 agcagatgaa gataaattta ataattatat accttatatg gattttcctt ttgaaaaaaa
      961 agataatggc tattattttg aaataggaag tagtgtaaaa cctgaaattt tatgtaaaga
//
LOCUS       DAOQAN010000613         1019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_244374_length_1019_cov_4.317427, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000613 DAOQAN010000000
VERSION     DAOQAN010000613.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_244374_length_1019_cov_4.317427"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="PKO10_08380"
     CDS             <1..307
                     /locus_tag="PKO10_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HNW67203.1"
                     /translation="NWANNIGINSLQWVKNEFKIVTHAPNAYRKLNAVLNLAKIYGNK
                     DLDLALEYALDNNVTATASIKSILDKKIYLQKPVNNTTTVTVELFNNHENLRGNIYE"
     gene            338..>1019
                     /locus_tag="PKO10_08385"
     CDS             338..>1019
                     /locus_tag="PKO10_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008338275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNW67204.1"
                     /translation="MTHTSVLNKITELGYKGFKEAYIRQMEDVNYHNLSFEDRLYQLL
                     DAQDAFLNNSRIQMNLKLSKIKNKQALIENIDYDPKREINKAQILSLASMDFIRSRQN
                     IIINGIAGVGKSTMAQAFGIRAIQSGFTVYYTRTATLLEDIKFARIDGSYTNLVKKFA
                     RYKLLILDDFGISPISVDDATNLFEIIETRNQLSSTIITSQLPVKDWYGYLQNNTVAD
                     AILDRVVHS"
BASE COUNT          439 a          125 c          146 g          309 t
ORIGIN      
        1 aaattgggca aataatattg gaataaatag tttgcaatgg gttaaaaatg agtttaaaat
       61 agttactcat gcaccaaatg catatagaaa actaaatgct gttttaaatt tagcaaaaat
      121 atatggtaat aaagatttag atttagcatt agagtatgca ttagataata atgtaacagc
      181 aactgcatca attaaatcta ttttagataa aaaaatctat ttacaaaaac cagttaataa
      241 tactacaaca gttacagtag aactatttaa taatcatgaa aatcttcgtg gaaacatcta
      301 tgaataaaat taaaaaataa aaagcagagg taaaaacatg acacacacat cagttttaaa
      361 taaaatcaca gaactaggtt ataaaggttt taaagaagca tatattagac aaatggaaga
      421 tgtaaactat cacaatctat ctttcgaaga tagattgtat caactcttag atgctcaaga
      481 tgctttttta aataatagta gaattcaaat gaatttaaaa ctctcaaaga ttaaaaacaa
      541 acaagcactt attgagaata ttgattatga tccaaaaaga gaaatcaata aagctcaaat
      601 cttatcttta gcttctatgg actttattag atctaggcag aacataataa taaatggaat
      661 agcaggagta ggaaaaagca caatggcaca agcatttgga attagagcta ttcaaagtgg
      721 atttacagtt tattatacaa gaacagcaac tttacttgaa gatataaagt ttgcaagaat
      781 agatggtagt tatacaaatt tagtaaaaaa atttgcaaga tataaacttc taatacttga
      841 tgactttggt atttcaccaa tatctgttga tgatgcaaca aatctatttg aaataattga
      901 aacaagaaat caattatctt caacaattat aacttcacaa cttccagtta aagattggta
      961 tggatatttg caaaataata ctgttgcaga tgcaatactt gatagagtag tacactcat
//
LOCUS       DAOQAN010000614         1019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_244570_length_1019_cov_2.099585, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000614 DAOQAN010000000
VERSION     DAOQAN010000614.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_244570_length_1019_cov_2.099585"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1019)
                     /locus_tag="PKO10_08390"
     CDS             complement(<1..>1019)
                     /locus_tag="PKO10_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNW67205.1"
                     /translation="ILRGIKERLETHHNVTINDSALVAAAKLSNRYITDRFLPDKAID
                     LIDEAAAELKMQIESEPTALSTIKREIQTLNVEKEALKMEKSQKNEDRIKEIEKELAN
                     KKEEKQNLEARFENEKQTFNATSELKIKIDELKTKANIAKRESKFEEAAKIEYGEIPA
                     LEAKIKENSEKWDRMQKEGTLLRNSVDEESIASIVSKWTGIPVNKMMDSEKQKVLKVE
                     EVLKKDVIGQDEAIKAISRAIKRNKAGLSETSRPIGSFLFLGPTGVGKTESAKTLAKF
                     LFDDPKSLIRFDMSEYMEKHAVSRLVGAAPGYVGYEEGGQLTEAVRRKPYSVILFDEV
                     EKAHP"
BASE COUNT          244 a          204 c          113 g          458 t
ORIGIN      
        1 tggatgtgct ttttcaacct catcaaaaag tattacacta taaggttttc ttctaacagc
       61 ctctgttaat tgaccaccct cttcatatcc aacataacca ggtgctgctc ctacaagtct
      121 tgaaacagcg tgtttctcca tatattcact catatcaaat ctaattagtg attttggatc
      181 atcaaataaa aatttagcta aagtttttgc actctcagtt tttccaactc ctgttggtcc
      241 taagaataag aagcttccta ttggtcttga agtttcactt aatcctgctt tatttctttt
      301 aattgctcta ctaattgctt ttattgcttc atcttgacct attacatctt tttttagaac
      361 ttcctcaact tttagaacct tttgtttttc actatccatc atcttattta caggaattcc
      421 agtccacttt gaaactatag aagcaatact ctcttcatct acactatttc gtaaaagtgt
      481 tccctctttt tgcattctat cccatttttc actattttct tttatttttg cttcaagtgc
      541 aggtatttca ccatactcta tttttgcagc ttcttcaaat tttgactcac gtttagctat
      601 atttgctttc gtttttagtt catcaatttt tatttttaat tcacttgtag cattaaaagt
      661 ttgcttctca ttttcaaatc tagcttctaa attttgtttt tcctcttttt tgtttgctag
      721 ctctttttct atctctttaa ttctatcttc gtttttttgg cttttttcca tttttaaagc
      781 ctctttttct acatttagag tttgaatctc tcttttaata gttgaaagag ctgttggttc
      841 actctctatt tgcattttaa gttcagctgc tgcttcatct attaaatcta ttgctttatc
      901 aggtaagaat ctatcagtta tatatctatt tgatagtttt gctgctgcaa ctaatgcact
      961 atcgtttata gttacattat gatgtgtttc aagtctctct tttatacctc ttaaaattt
//
LOCUS       DAOQAN010000615         1018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_244826_length_1018_cov_3.047767, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000615 DAOQAN010000000
VERSION     DAOQAN010000615.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_244826_length_1018_cov_3.047767"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..523)
                     /locus_tag="PKO10_08395"
     CDS             complement(83..523)
                     /locus_tag="PKO10_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014473410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HNW67206.1"
                     /translation="MFKKLFLILCLFGFALAETKFSDPKPSFESPRKVVYSLYVNDLD
                     TVNHTIGSMYNILKEYPAESLKIVVVAYGKGLRALKKDYDKETLKRIKSLMEYDVEFV
                     ACKNTMETMKWKESDFIDDISFTQAGIVEVIERQVDGYIGITAY"
     gene            complement(523..966)
                     /locus_tag="PKO10_08400"
     CDS             complement(523..966)
                     /locus_tag="PKO10_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014473409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HNW67207.1"
                     /translation="MKILGEVLEVFSATKESSGLPRPKVESLNLIKDYGIEFDKFAEK
                     NLDQTVMIVGLKSYELAKSFNIDLELGSLGENILLDFDPHDFEVGKYFKIDEAIIQIT
                     QICTVCNHLTVFNKDLPKILKGHRGVYCKIIESGKILKNMKVKEV"
BASE COUNT          350 a          181 c           91 g          396 t
ORIGIN      
        1 tttgcaaaaa gataattcac aaataccaat gaagccaaaa ttactaattt tttcatattt
       61 ttattctcct ttaaaaatag ttttaatacg ctgtaatacc aatatatcca tcaacttgtc
      121 gttctattac ttcaacaatt ccagcttgag taaatgaaat atcatctata aaatcactct
      181 ctttccattt catagtttcc atagtatttt tgcaagcaac aaattcaaca tcgtactcca
      241 tcaaagattt tattcttttt aatgtctctt tatcataatc tttttttaga gctctaagac
      301 cttttccata agcaacaact acaattttta gactttcagc tggatactct tttaaaatat
      361 tgtacataga accaatagta tgatttacag tgtctaaatc atttacatat agtgaatata
      421 caacttttct tggtgattca aagctaggtt ttggatcgct aaattttgtc tctgccaagg
      481 caaaaccaaa tagacaaaga attaaaaaga gttttttaaa cattatactt cctttacttt
      541 catatttttt aaaatctttc cagattctat aatcttacaa taaactcccc tatgaccttt
      601 taaaattttt ggtaaatctt tattaaaaac agttaaatga ttgcaaactg tacatatttg
      661 agttatttga attatagctt catctatttt gaaatatttt ccaacttcaa aatcatgagg
      721 atcaaaatct aataaaatat tctcacccaa acttcctaac tctaaatcta tattaaatga
      781 ttttgctaat tcatatgatt ttaaaccaac aatcataaca gtttggtcaa gattcttttc
      841 tgcaaattta tcaaactcta ttccataatc ttttattaag tttaagcttt caacttttgg
      901 tctaggaagt ccacttgact cttttgttgc actaaaaact tctaaaactt cacctaaaat
      961 tttcatttgt tctcttttaa aatatctata aaatcatttt tcttccaagc accaggat
//
LOCUS       DAOQAN010000616         1016 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_245663_length_1016_cov_2.650364, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000616 DAOQAN010000000
VERSION     DAOQAN010000616.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_245663_length_1016_cov_2.650364"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="PKO10_08405"
     CDS             <1..335
                     /locus_tag="PKO10_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW67208.1"
                     /translation="TTIEKEATNYWKKEFLEPKIVDNNISYSLKKIDKIVLSNTLGND
                     KPQIIAECLGIDYLEDESKFKIYLGKILEQKNINNFKDKKDILISELINEKNELIKIL
                     ENIKKSIK"
     gene            complement(366..644)
                     /locus_tag="PKO10_08410"
     CDS             complement(366..644)
                     /locus_tag="PKO10_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNW67209.1"
                     /translation="MKKIVLATTILAATFAFANPYAKCVACHGANGEKVALGKSKIIK
                     DMTKEDFVASLKGYQDGTYGGAQKALMVGQVKGMDEATMKQIADLIIK"
     gene            complement(721..1011)
                     /locus_tag="PKO10_08415"
     CDS             complement(721..1011)
                     /locus_tag="PKO10_08415"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HNW67210.1"
                     /translation="MKQTFEVHNVKCGGCANTLTKSLKDDFGSVEVDLSVNPRKITLD
                     IEDNKIEELKLKLRSLGYPLTNDELSDFEKATTTAKSFVSCAIGKIDVALNK"
BASE COUNT          385 a          138 c          111 g          382 t
ORIGIN      
        1 taacgactat cgaaaaagaa gctactaatt attggaaaaa agagttttta gagccaaaaa
       61 ttgtagataa taatatttct tatagcctaa aaaagataga caaaatagtt ttatctaaca
      121 cattaggtaa tgataaacca caaattattg cagaatgttt aggtattgat tatttagaag
      181 atgaatcaaa gtttaaaata tatttgggaa aaattttaga acaaaaaaat ataaataatt
      241 ttaaagataa aaaagatatt ttgataagtg aacttataaa tgagaaaaat gagcttataa
      301 aaatattaga aaatataaaa aagagtataa agtagctaaa tattttagct actttattaa
      361 aaagattact taattattaa atcagcaatc tgtttcatag ttgcttcatc cataccttta
      421 acttgaccaa ccattagagc tttttgagca ccaccataag ttccatcttg ataacctttt
      481 aaagatgcta caaaatcttc ttttgtcata tctttgatta ttttagattt acctaaagcc
      541 actttttcac catttgcacc atgacatgct acacattttg cataaggatt tgcaaatgca
      601 aaagttgccg ctaatattgt tgttgctaaa actatttttt tcatttaatt tcccttttaa
      661 ataagataaa attattatat gcaatctaag attaaaatat attgatttta gtcaattttt
      721 ttatttattt aatgcaacat caattttacc aatagcacaa gagacaaaac tttttgcagt
      781 tgtagttgct ttttcaaaat cacttaactc atcgtttgtt agtggatatc ctaaacttct
      841 taatttaagt tttaactctt caattttatt atcttcaata tctaatgtta tttttcttgg
      901 atttacagat aaatcaactt caacactacc aaaatcatct ttcaaagatt ttgttaaagt
      961 atttgcacaa ccaccacatt ttacattatg tacttcaaat gtttgtttca tttatt
//
LOCUS       DAOQAN010000617         1016 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_245820_length_1016_cov_1.824142, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000617 DAOQAN010000000
VERSION     DAOQAN010000617.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_245820_length_1016_cov_1.824142"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..963)
                     /locus_tag="PKO10_08420"
     CDS             complement(<1..963)
                     /locus_tag="PKO10_08420"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNW67211.1"
                     /translation="MIKLNNFQKASTILKKIREEEKNSEILNKVLWISAYSSLKQNNF
                     EDAQEILDLINDRKKDFNVNIQMPLEIFFNKDLYTYKDYYKSIMKFDEDRMYDFIYYF
                     APFVFSDSKEIIYDSVKGFIYGKAQSVENLENMVEYNTKFIRLVKQDPIKRVNELKNI
                     IKTDSKAYIYYNLALSYAQINDFTNAYKNFEKAYKLSPGNKLYSVMYLITANKISADI
                     PDKQRAILDKNIKDSGGLYSYFAKELYKLFVNSTYNVVEASQSYEKTVFYKAIDFLKK
                     MNNNENLSNHQLLEDYERDPFIYLLKLVQKNKNEDDYRYAARVQD"
BASE COUNT          337 a          139 c           95 g          445 t
ORIGIN      
        1 atcttgaact cttgctgcgt atctataatc atcttcattt ttattttttt gaacaagttt
       61 taaaagatat ataaaagggt ctctctcata atcttcaaga agttgatgat tacttaaatt
      121 ttcattatta ttcattttct ttagaaaatc tatagcttta taaaaaactg ttttttcata
      181 agattgtgat gcttcaacaa cattgtaagt actatttaca aataatttat ataactcttt
      241 tgcaaagtag ctatataatc ctccactatc ttttatattt ttatctaata tagctctttg
      301 tttatctggt atatcagcag atattttatt tgcagtaatt aggtacataa cagaatacaa
      361 cttattacca ggacttagtt tatatgcttt ttcaaagttt ttataagcat ttgtaaaatc
      421 attgatttgt gcataagata gagctaggtt atagtaaata tatgctttag aatctgtttt
      481 tataatattt tttaattcat ttactcgctt tattggatct tgttttacca aacgaataaa
      541 ttttgtatta tattcaacca tattttctaa attctctaca ctttgtgctt tgccatatat
      601 aaaacctttt acagaatcat atattatctc ttttgaatct gaaaatacaa aaggagcaaa
      661 ataatatata aaatcataca ttctatcttc atcaaatttc ataatacttt tgtagtaatc
      721 tttgtatgta tataaatctt tattaaaaaa tatctctaga ggcatctgta tatttacatt
      781 aaaatctttt tttctatcat taattaaatc aagtatctct tgtgcatctt caaaattatt
      841 ttgtttcaaa ctagaatatg ctgaaatcca caatacttta tttaaaattt cactattttt
      901 ctcttcttct cttatctttt tcaaaattgt tgaagctttt tgaaaattat ttagttttat
      961 caaaatcata gcttttttta agttttacat ttcgacaatc tgattctaaa aattta
//
LOCUS       DAOQAN010000618         1011 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_247767_length_1011_cov_1.927824, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000618 DAOQAN010000000
VERSION     DAOQAN010000618.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_247767_length_1011_cov_1.927824"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..977
                     /locus_tag="PKO10_08425"
     CDS             <1..977
                     /locus_tag="PKO10_08425"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNW67212.1"
                     /translation="TKNKDNLKEIRDICTNIIEYFSLHPILINNNSFYLSISIGVARN
                     CKDSAVQNNLLSKAEYALRIAKKRDISILFLDENIELYNKLKENKKLIEELKNALISN
                     NLLIYGQKLINNISKKEKYEILMRVKLEDGSILTPYSFLKEAKKAKLYLGMTRMLVKK
                     ACEYFKGKDIDFNLNLTLEDIKDEYTMDFIVNAMEKTNTAKQITFEIVESEGIESFTE
                     VSNFIKKAKKLGCKIAIDDFGTGYSNFEYIIKLDVDYIKIDGSLIKNINTDNNLYLTV
                     QTIVGFAKALKIKTVAEFVHNEEVLNCVKNLDIDYSQGFFIDEPKELA"
BASE COUNT          434 a           92 c          149 g          336 t
ORIGIN      
        1 gtacaaaaaa taaagataac ctaaaagaga taagagatat atgtacaaat attattgaat
       61 atttttcttt acaccctatt ttaataaata ataatagctt ttatttaagt atctctattg
      121 gtgttgctag aaattgtaaa gatagtgctg ttcaaaataa tcttttgtca aaagcagaat
      181 atgcactaag aatagctaaa aaaagagata tttcaatact atttttagat gaaaatattg
      241 agctttataa taaattaaaa gagaataaaa aactaattga agagttaaaa aatgctctta
      301 tttcaaataa tcttttaata tatggtcaaa agctaattaa taatatttct aaaaaagaga
      361 aatatgagat attaatgaga gttaaactag aagatggttc tattttaaca ccatatagtt
      421 ttttaaaaga ggcaaaaaaa gcaaaactat atctaggaat gacaagaatg cttgtaaaaa
      481 aagcttgtga atatttcaaa ggtaaagata ttgactttaa cttaaattta actcttgaag
      541 atattaaaga cgagtatact atggatttta ttgtgaatgc aatggaaaag acaaatactg
      601 caaaacagat aacttttgag atagttgaaa gtgaagggat tgaaagtttc actgaagtat
      661 caaattttat aaaaaaagca aaaaaacttg gttgtaaaat tgctattgat gattttggta
      721 ctggatactc aaattttgag tatattataa aattagatgt tgattatata aaaatagatg
      781 gctctttaat aaaaaatata aatacagata acaatctata tcttactgtt caaactattg
      841 ttggatttgc aaaagcactt aaaataaaaa ctgttgcaga gtttgtacat aatgaagagg
      901 ttttaaattg tgtaaaaaat ctagatatag attactctca aggttttttt atagatgagc
      961 caaaagagtt ggcttagtag taaaatagaa tactaactat taacaaactt t
//
LOCUS       DAOQAN010000619         1009 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_248606_length_1009_cov_1.611111, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000619 DAOQAN010000000
VERSION     DAOQAN010000619.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_248606_length_1009_cov_1.611111"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="PKO10_08430"
     CDS             complement(<1..214)
                     /locus_tag="PKO10_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3944 domain-containing protein"
                     /protein_id="HNW67213.1"
                     /translation="MKYQKDKDLEFLKNVKSQDLENLVQILTSTKTEELSKRDIYKEF
                     YPNHSIYWEEIAAELQYFGGNTVVNII"
     gene            462..695
                     /locus_tag="PKO10_08435"
     CDS             462..695
                     /locus_tag="PKO10_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNW67214.1"
                     /translation="MKNLTPDELYKIIGQNVAKYRKEKGLSQLDLSLAMGYKSVSVVS
                     GAEIYYNGKHFNLEHLLKISQILNIEISEFLKP"
     gene            complement(718..>1009)
                     /locus_tag="PKO10_08440"
     CDS             complement(718..>1009)
                     /locus_tag="PKO10_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW67215.1"
                     /translation="SNLDEEIIDETVKQNIKVTIDLLFKANNILSDIVLNLNESLKDK
                     FDYESFDIKEQELIKEAFLLAKFIQKVCINDDLTEDVVVKFNNIISNIEESV"
BASE COUNT          368 a          143 c           93 g          405 t
ORIGIN      
        1 ttataatatt tactactgta ttacctccaa aatattgaag ttcagctgct atttcttccc
       61 aatatatact atgattagga taaaattctt tataaatatc tcttttagat aattcttctg
      121 ttttagtgct agttaaaatt tgtactaagt tctctaaatc ttgagatttt acatttttta
      181 aaaattctaa atctttgtct ttttgatatt tcatcttatc ccctttgtgt gaaacaattt
      241 attatagtca aaactactat taaatatata taaaaagtaa aaaataaaat aagaaaatct
      301 aatttattac ttttctatac gcaaaaacat ctttagtaaa aaaatattta cttttctctt
      361 tttatctctt atagtacatt ttcttcataa agattgtaaa caattctaca taagataaac
      421 ttacaagcaa tttatcacta atagtaggtt gtaaaaatca tatgaaaaac ttaactcctg
      481 atgagcttta taaaattatc ggtcaaaatg ttgccaaata tagaaaagaa aaaggcttat
      541 ctcagcttga tttatctttg gctatgggat acaaatctgt ttcagtagta tctggtgcag
      601 agatatatta caacggcaaa catttcaacc tagaacatct tttaaaaata tcacaaatat
      661 taaatataga gatatcagag tttttaaaac cctgatactt ctatttatat tttattatta
      721 aacactctct tctatatttg aaataatatt gttaaattta acaacaacat cttcagtcaa
      781 atcatcattt atacaaactt tttgaataaa ctttgcaagt aaaaaagcct cttttataag
      841 ctcttgctct ttaatatcaa agctttcata atcaaactta tcttttagag attcatttag
      901 atttaaaact atatcactta ggatattgtt tgctttaaat agtaaatcta tagtcacttt
      961 tatattttgt tttacagttt catctattat ttcttcatct aaatttgaa
//
LOCUS       DAOQAN010000620         1007 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_249249_length_1007_cov_2.097689, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000620 DAOQAN010000000
VERSION     DAOQAN010000620.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_249249_length_1007_cov_2.097689"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(142..627)
                     /locus_tag="PKO10_08445"
     CDS             complement(142..627)
                     /locus_tag="PKO10_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004510370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HNW67216.1"
                     /translation="MSSFKYTPEDEAKFQEYASRYPKIDSCMLPALWLVQEQEGWVSP
                     EAMVYVAEKVQKSPMQVYEVATFYTMFNLKPIGKYHIELCKTVSCMLMGARELKAFIK
                     DTLGLEPGQTSADGLFTFSEVECQGACGDAPMIALNNVYHGKLTKEKLEKIIWECKND
                     N"
     gene            complement(627..>1007)
                     /locus_tag="PKO10_08450"
     CDS             complement(627..>1007)
                     /locus_tag="PKO10_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012012152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HNW67217.1"
                     /translation="IQSIKIIRQAMKNMPDGAINVYAPNLVLPNKKDVYGNIEGLMNQ
                     FKLTFEGTLVPKGEYYSSTEATNGELGFFIISDGSGRPYKVKCRPPCFYSLAAYSKIV
                     EGTMLADAVVTMASLNFIAGEFDR"
BASE COUNT          316 a          196 c          130 g          365 t
ORIGIN      
        1 cgtagccctg atgcagtaac ttcagcagtt acctcttcag gtttcatagt gaaaagttta
       61 tctattgaag agtatcttcc atttgcaaga gcaacttcaa gtttgtgtga atttgggata
      121 tcaaaatttt tacttactat tttagttatc atttttgcac tcccaaatta ttttttctaa
      181 tttctctttt gttaattttc cgtgataaac attgtttaat gcaatcattg gagcatctcc
      241 acaagcacct tgacactcaa cttcactaaa agtaaaaagt ccatcagcac ttgtttgacc
      301 aggttctaat cctaaagtat cttttataaa agctttaagt tcacgtgcac ccattaacat
      361 acaagataca gttttgcata gctcaatatg atattttcca ataggtttta agttaaacat
      421 tgtataaaaa gttgccactt catatacttg cattggactt ttttgaactt tttctgctac
      481 ataaaccata gcttctgggc ttacccaacc ttcttgctct tgtaccaacc aaagagctgg
      541 tagcatacaa gagtcaattt ttggatatct agaagcatac tcttgaaatt ttgcttcatc
      601 ttctggtgta tacttaaaac tactcattat ctatcaaact ccccagcaat aaaattcaag
      661 ctagccattg taacaacagc atcagctaac atagtacctt caactatttt tgaataagca
      721 gccaaagaat aaaaacatgg tggtctacat ttaactttat atggtcttcc gcttccatca
      781 cttataataa agaaacctaa ctctccattt gtagcttctg ttgaactata atattcacct
      841 tttggaacta aagttccttc aaatgtaagc ttaaattgat tcattaaacc ttcaatattt
      901 ccataaacat cttttttatt tggtaataca agatttggag cataaacatt tatagcacca
      961 tctggcatat ttttcatagc ttgtctaatg atttttatag attgaat
//
LOCUS       DAOQAN010000621         1007 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_249320_length_1007_cov_1.860294, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000621 DAOQAN010000000
VERSION     DAOQAN010000621.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_249320_length_1007_cov_1.860294"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1007
                     /locus_tag="PKO10_08455"
     CDS             <1..>1007
                     /locus_tag="PKO10_08455"
                     /inference="COORDINATES: protein motif:HMM:NF019421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HNW67218.1"
                     /translation="PLKLPLSLEGVYTNNDEHYFEGLAGVFHDSLPDKFGTKVIERYF
                     ESKNIPPHELTVIQKLMFVGDKSIGAITYKPVAHKIEEKINELIELQNFYENAKKIIS
                     GDAIEVVDEMLNFMDSAASAGGARAKAIIGYNCKTQEITSGVKRELKKDFDHYLIKFD
                     IQKYDGSSSNYTKLEYLYMQMAKEVGIDVPKIELLSHGNLAHYLIKRFDRVNGEALHL
                     HSVAGLTHTNFNIPMHYSYDELLRLTRYLTGSQQAVNEQLKRMIFNIVGRNQDDHAKN
                     FAFMMDKNGIWNLSPAYDITYSNGTGYTKNHQLSLNGKTNDFTLNDILQLAKKHSIKE
                     N"
BASE COUNT          402 a          137 c          169 g          299 t
ORIGIN      
        1 caccactaaa acttccttta tctcttgaag gtgtatatac aaacaatgat gaacactatt
       61 ttgaaggctt agctggagtt tttcatgata gtttgcctga taaatttggt acaaaagtaa
      121 ttgaaagata ttttgaatca aaaaatattc cgccacatga actaactgtt atccaaaagc
      181 ttatgtttgt aggagataaa agtatagggg ctattacata caaacctgtt gctcataaga
      241 tagaagaaaa aatcaatgaa ctgatagaac tccaaaactt ttatgaaaat gcaaaaaaga
      301 ttataagtgg cgatgctatt gaagtagtgg atgaaatgct taattttatg gatagtgctg
      361 caagtgcagg aggagcaaga gctaaagcaa ttataggata taactgcaaa acacaagaaa
      421 taacaagcgg tgttaaaaga gaattaaaaa aagattttga ccactattta attaaatttg
      481 atattcaaaa atatgatggt tctagcagta attatacaaa actggaatac ctttatatgc
      541 aaatggcaaa agaggttggt atagatgtac caaaaattga gttattaagt catggaaatt
      601 tagcacatta cctcataaaa agatttgata gagtaaatgg tgaagcttta catttacact
      661 ctgtagcagg acttacccat acaaatttta atattcctat gcattactct tatgatgaac
      721 tcttacgact cacaagatac cttacaggaa gccagcaagc agtaaacgaa caacttaaaa
      781 gaatgatttt taatattgtt gggcgaaatc aagatgatca tgctaaaaat tttgctttta
      841 tgatggataa aaatggaatt tggaatttaa gcccagcata tgatataact tattcaaatg
      901 gaactggata tacaaaaaat catcaactct ctttaaatgg aaaaactaat gattttactt
      961 tgaatgatat tttacagtta gcaaaaaaac actccataaa agagaat
//
LOCUS       DAOQAN010000622         1005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_250039_length_1005_cov_2.154737, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000622 DAOQAN010000000
VERSION     DAOQAN010000622.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1005
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_250039_length_1005_cov_2.154737"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1005)
                     /locus_tag="PKO10_08460"
     CDS             complement(<1..>1005)
                     /locus_tag="PKO10_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014474339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C biogenesis protein"
                     /protein_id="HNW67219.1"
                     /translation="LIEDEVNKFKDESRVTADKFGHLVVQSSGGRMKPLATLNRDIVQ
                     KLSGKSSFMGMDANQIVLGMITRPDIWKDVKIIKINTPKLKKFLGVPESEKYISFSEA
                     FGEKNDYLLAKESEKALLTKPIERGTYEKDIIKVDEKLNIIYSVFNGALLNIFPKVYD
                     EQSVDDNFKWYSPIEAMQEFSGQNQAAIGSVVRGLFNSTMDFEWNSANNYIDMIALYQ
                     DKVGTDIKPTASKINAEIVFNKLDIFFNLTLAYVLLGFIMVVLAFVVIFKPEFKPAKT
                     TKIIFAILTILFAIQTFGMGYRWYLSGHAPWSDIYETLIYISWSAIFAGVIFFRNSLL
                     A"
BASE COUNT          348 a          168 c          108 g          381 t
ORIGIN      
        1 agctaataaa gagtttctaa aaaatataac tccagcaaat atagcagacc aagatatata
       61 aattaaagtt tcataaatat cactccaagg tgcatgtcct gataaatacc atctataacc
      121 cataccaaaa gtttgtatag caaaaagtat agttaaaata gcaaatataa tttttgttgt
      181 ttttgcaggt ttaaactctg gtttaaatat aacaacaaaa gctaaaacaa ccataataaa
      241 acctaaaaga acgtaagcta aagttaaatt aaagaaaata tctaatttat taaaaactat
      301 ttcagcattt atttttgaag cagtaggttt tatatctgtt ccaactttgt cttgatataa
      361 agctatcata tcaatataat tatttgcact attccattca aaatccatcg ttgagttaaa
      421 tagtcctctt acaacagatc caatagcagc ttgattttgt cctgaaaatt cttgcattgc
      481 ttcaataggt gagtaccatt tgaagttatc atctacactt tgttcatcat aaacttttgg
      541 aaaaatattt aatagtgcac cattaaatac gctataaata atatttaatt tttcatctac
      601 ttttataata tctttctcat aagttcctct ttcaattggt tttgtaagaa gagctttttc
      661 tgactctttt gcaagtaaat aatcattttt ttctccaaat gcttctgaaa aagagatata
      721 tttttcactt tcaggtactc ctagaaattt tttaagtttt ggagtattta tttttattat
      781 ttttacatct ttccaaatat caggtcttgt aatcatccct aaaacaatct gatttgcatc
      841 cattcccata aatgatgatt ttccacttag tttttgaact atatctctat ttaaagttgc
      901 aagaggtttc attcttccac cgctactttg cactactaaa tgtccaaatt tatctgcagt
      961 tactcttgat tcatctttaa atttatttac ctcatcctca atcaa
//
LOCUS       DAOQAN010000623         1001 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Aliarcobacter cryaerophilus isolate AMR_MDS_1261
            NODE_251539_length_1001_cov_3.650106, whole genome shotgun
            sequence.
ACCESSION   DAOQAN010000623 DAOQAN010000000
VERSION     DAOQAN010000623.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854466
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Aliarcobacter cryaerophilus (wastewater metagenome)
  ORGANISM  Aliarcobacter cryaerophilus
            Bacteria; Campylobacterota; Epsilonproteobacteria;
            Campylobacterales; Arcobacteraceae; Aliarcobacter.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:21:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,672
            Genes (coding)                    :: 1,647
            CDSs (with protein)               :: 1,647
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 10 of 25
            Pseudo Genes (incomplete)         :: 9 of 25
            Pseudo Genes (internal stop)      :: 7 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Aliarcobacter cryaerophilus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_251539_length_1001_cov_3.650106"
                     /isolate="AMR_MDS_1261"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:28198"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /locus_tag="PKO10_08465"
     CDS             <1..814
                     /locus_tag="PKO10_08465"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011372904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HNW67220.1"
                     /translation="DGRINYDKVQEIAKIVQPKIIVCGASAYAREIDFKRFREIADSV
                     GAILFADIAHIAGLVAAGEHQSPFPHAHVVTTTTHKTLRGPRGGMIMTNDEEIAKKIN
                     SAIFPGLQGGPLVHVIAAKAVAFKEILDPKWKDYAKQVKANAKVLGEVLVSRGYDIVS
                     GGTDNHLVLVSFLNKPFSGKDADAALGDAGITVNKNTVPGETRSPFVTSGIRIGSPAL
                     TARGMKEKEFEYIANKICDVLDNIEDKELHKKINKELEELASKFVIYKSSTY"
BASE COUNT          385 a          119 c          204 g          293 t
ORIGIN      
        1 tgatggaaga ataaattatg ataaagtgca agagattgca aaaatagttc aaccaaaaat
       61 tattgtttgt ggtgcaagtg catatgctag agagattgac tttaaaagat ttagagaaat
      121 agctgattca gttggtgcta ttttatttgc agatattgct catattgctg gtcttgttgc
      181 tgcgggagag catcaatctc cattccctca tgctcatgtt gtaacaacaa ctactcataa
      241 gactttaaga ggtccaagag gtggtatgat tatgacaaat gatgaagaga ttgctaaaaa
      301 aattaactct gctattttcc ctggacttca aggaggtcca cttgttcatg taattgctgc
      361 taaagctgtt gcatttaaag agatacttga tcctaaatgg aaagattacg caaaacaagt
      421 aaaagctaat gcaaaagtat taggtgaagt tctagtaagt agaggttatg atattgtaag
      481 tggtggaaca gataatcact tagttttagt atcattctta aataaaccat tctcaggaaa
      541 agatgcagat gcagcacttg gtgatgctgg tataactgta aataaaaaca cagttccagg
      601 tgaaacaaga agcccatttg taacttcagg tattagaata ggaagcccag ctttaacagc
      661 acgtggaatg aaagagaaag agtttgaata tattgcaaat aaaatttgtg atgtattaga
      721 taatattgaa gataaagagt tacacaaaaa aataaacaaa gagcttgaag agttagctag
      781 taagtttgtg atttataaaa gttcgacgta ttaataaagt ttagaagtaa ggagttatta
      841 tgcaaataat tggtgatgat tttgtaaaaa aagttcaact aaaacaagaa aaagaagagc
      901 ttgagaagaa gttaagtgaa cttgatgaaa caaggatatc tatgggaaat ataaatctta
      961 caaacaatct aaataatgcc tataagggta tagataataa t
//
